ubr5 cDNA ORF clone, Danio rerio(zebrafish)

The following ubr5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ubr5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa33864 NM_001164394.1
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149204 XM_021466626.1
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149205 XM_021466627.1
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149206 XM_021466628.1
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149207 XM_021466629.1
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149208 XM_021466630.1
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa149209 XM_021466631.1
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa22029 XM_005158437.4
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa22030 XM_005158438.3
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa22031 XM_005158439.3
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa22032 XM_005158440.4
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa22033 XM_005158441.3
Latest version!
Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa22032 XM_005158440.3 Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa22029 XM_005158437.3 Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa33864
    Clone ID Related Accession (Same CDS sequence) NM_001164394.1
    Accession Version NM_001164394.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8385bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR5
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CR293507.10. On Aug 22, 2009 this sequence version replaced XM_001923253.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA3505370, SAMEA3505380 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGA
    GGAGGATCTT CAGGACGTTC ATCCACAGCA GCCCGTGATT CACGCAGGCA GACCCGCGTC
    ATCCGCACCG GCAGAGATCG AGGCTCTGGC CTGCTGGGAA GCCAACCACA GCCTGTGATT
    CCAGCCTCCG TCATCCCCGA GGAGCTCATC TCTCAGGCTC AGGTGGTTCT TCAGGGGAAA
    TCCAGAAGTG TCATCATTCG AGAGCTGCAG CGCACAAACC TTGACGTGAA CTTGGCTGTA
    AACAACCTGC TGAGCAGAGA TGATGAGGAT GGAGATGATG GCGATGACAC GGCCAGCGAA
    TCCTACTTAC CTGGAGAAGA CTTGATGTCG CTGCTAGATG CAGACATTCA CTCCGCTCAC
    CCCAGCGTGA TCATAGATGC CGATGCCATG TTCTCTGAGG ACATCAGCTA CTTCGGTTAC
    CCCTCTTTTC GTCGCTCCTC CTTGTCTCGT CTTGGCTCCT CACGAGTTCT CCTTCTCCCC
    CTAGAGAGAG ACTCTGAGCT GCTCCGCGAG CGTGAGTCGG TCCTGAGGCT GCGGGAGAGG
    CGGTGGCTGG ACGGGGCCTC CTTCGATGCG GAGCGTGGCT CCACCAGCCG CGAGGGAGAG
    CCCAGCCTTG ACAAGAAGAG TGTCCCGCTG CAGAGCCCAG TCACTCTGGG GGAAGAGCTC
    CAGTGGTGGC CTGATAAGGA CTATGTAACA AAGTTTGTCA GCATTGGTGC TTTGTACTCG
    GAGCTGGTTG CCGTCAGTAC CAAAGGAGAA CTCTACCAGT GGAAGTGGAA TGAACCTGAA
    CCTTACAGAA ACGCACAAAA TCCTTCTATA CATCACCCTC GTGTTCCTTT CCTGGGCTTG
    ACCAATGAAA AGATCACCCA CCTGTCCGCC AACAGTATCC GAGCCACTGT GGCCACCGAG
    AACAACAAGG TGGCAACATG GGTGGATGAA ACTCTGAGCA CTGTTGCTGC TAAACTCGAA
    CACGGAGCAC AAACGTTCCC GGAGCTGCAG GGGGAGCGGA TCGTCTCTCT GCACTGCTGT
    GCCCTGTATA CCTGCGCTCA GCTGGAGAAC AGCCTTTACT GGTGGGGTGT TGTGCCTTTT
    AGTCAACGGA AAAAAATGCT TGAAAAGGCC AGAGCCAAGA ACAAGAAACC AAAGTCCAGT
    GCTGGAATCT CTTCGATACC CAACATCACA GTAGGAACTC AGGTGTGCCT GAGGAATAAT
    CCTCTTTATC ACGCTGGTGC CGTGGCCTTT TCCGTGAACG CCGGGATCCC TAAAGTGGGC
    GTCCTGCTGG AGTCGGTGTG GAACATGAAC GACAGCTGCC GCTTCCAGCT GCGCTCGCCA
    GAGAGCCTGA AAAACATGGA GAAAAACACC AAGACACAGG AGACCAAGAC GGAGAGCAAG
    CCAGAGCTGG TGAAGACGGA AATGGGCCCT CCGCCCTCAC CAGCGTCCAC ATGCAGTGAC
    ACTTCATCCA TCGCTAGCAG CGCTTCACTG CCGTACAAGC GAAGGCGCTC AACACCGGCT
    CCGAAGGAGG AGGAGAAGGT GAATGAGGAG CAGTGGCCTC TGCGAGAGGT TGTTTTCGTG
    GAGGATGTTA AAAACGTCCC CGTGGGAAAG GTGCTAAAAG TCGATGGTGC ATATGTTGCT
    GTGAAATTTC CAGGGACGTC GAGCAGCGTG AGCAGCCAGA GCGCAGCTCC CACTGACTCT
    GACCCGTCCT CACTGCTGCA AGACTGTCGA CTGCTCAGAA TAGATGAGCT ACAGGTTGTT
    AAAACTGGTG GAACACCAAA AGTTCCTGAC TGTTTTCAGC GAACACCTAA AAAACTTTGC
    ATCCCAGAAA AAGCTGAAAT TTTAGCAGTG AATGTTGACT CCAAAGGAGT TCATGCTGTG
    TTGAAAACGG GCAGCTGGGT GAGATACTGT GTCTTTGACT TAGCCACCGG CAAAGCCGAG
    CAAGAGAATC ACTTTCCCAC CAGTAACCTG GCGTTTCTTG GTCAGAGCGA GCGAAATGTG
    GCCATCTTCA CTGCTGGCCA GGAGAGTCCC ATTATTTTGA GGGATGGCAA TGGCACTATT
    TATCCCATGG CTAAAGACTG TATGGGTGGA ATACGTGACC CTGATTGGCT GGATCTGCCG
    CCTATAGCCA GTCTGGGCAT GGGAGTTCAC TCGCTCGCCA ACCTTCCCAG TAACTCCACA
    ATCAAAAAGA AGGCGGCCAT CATCATAATG GCTGTAGAGA AGCAGACTCT GATGCAGCAC
    GTGCTGCGCT GTGATTACGA GGCGTGTAGG CAGTATCTGA TCAGTCTGGA GCAGGCCATG
    CTACTGGAGC AGAACCCTCA CGCTCTGGAC ACTCTGCTAG GACACCGCTG TGACGGAAAC
    CGCAACGTCC TCCATGCCTG TGTGTCCGTC TGCTTCCCCG TCAGCAACAA GGAAACCAAG
    GAGGAAGAAG AAGCAGAGCG GTCTGAAAGA AACACGTTCG CTGAGAGGCT TTCTGCAGTG
    GAGGCCATTG CCAATGCCAT ATCAGTGGTG TCGAGCAACA GCTCCGGGAA CCGGACAGGA
    TCATCCAGCA GCAGGGGCTT GCGTTTGAGA GAGATGATGC GGCGCTCACT CCGTGCTGCA
    GGTTTAGGCC GACATGAATC CGGACCTTCC TCCAGCGACC ATCAAGACCC CGTTTCACCC
    CCGATAGCAC CTCCTAGCTG GGTACCAGAC CCTCCACCGA TGGATCCTGA CGGTGACATT
    GATTTCATCT TGGCCCCTGC AGTGGGCTCC CTCACCACTG CTTCCACAGG CACCAGCCAG
    GGACCCAGCA CTTCCACCAT CCCAGGACCC TCCTCTGAAC CATCAGTAGT GGAATCTAAA
    GACCGTAAGG CGAACGCACA CCTGATACTG AAGCTGATGT GTGACAGCAT CGTCCTGCGG
    CCTCATCTAA GAGAACTGCT CTCTGCCAAG GATGCTCGTG GAATGACTCC ATTCATGTTA
    GCAGTGAGTG GCAGAGCTTA CCCAGCAGCC ATTACTGTCT TAGAAGCAGC ACAGAAAATT
    GCCAAAGGTG AACCAGGTCT TGGAGAGAAA GAGGATACAG CATCTGTGTT CATGGAGATG
    ATCTGTCCTT CAGGAACCAA TCCAGACGAC TCTCCGCTGT ACGTCCTGTG CTGTAACGAC
    ACCTGCAGCT TCACCTGGAC TGGAGCAGAA CACATCAACC AGGACATCTT TGAGTGCAGA
    ACTTGTGGCC TGCTGGAGTC GCTGTGCTGC TGCACTGAAT GTGCTAGAGT CTGTCACAAG
    GGGCACGACT GCAAACTGAA GAGGACGTCA CCAACAGCTT ACTGTGACTG CTGGGAGAAG
    TGCAAATGCA AGACTCTCAT CGCAGGCCAG AAAGCGGCAC GTCTGGATCT GCTCTACAGG
    CTGCTGACCA CCACTAATCT AGTGACCACT CCTAACAGCA GGGGAGAGCA CATCTTGCTG
    TTTCTGGTGC AGACTGTTGC AAGGCAGAGT GTCGAACACT GTCAGTATCG ACCTCCTCGC
    ATTCGAGAGG ACAGGAACCG GAAAGCTGCC AGTGCTGAAG ACTCTGATAT GCCAGATCAT
    GACCTGGAAC CTCCACGCTT TGCTCAGCTG GCTCTGGAGC GAGTGTTGCA GGACTGGAAC
    GCACTGAAGT CAATGATAAT GTTCGGCTCT CAGGAAAATA AAGATCCGTT AAGCGCTAGC
    AGCAGAATCG CTCACCTTTT ACCGGAGGAG CAGATGTACC TCAACCAGCA GAGTGGCACC
    ATCAGACTGG ACTGTTTCAC ACACTGCCTT ATTGTCAAGT GTGCACCAGA CATCACTTTC
    ATTGACACAC TGTTAGGGAC CCTGGTGAAG GAGCTGCAGA ATAAATACAC CCCCGGCCGG
    CGGGAAGAGG CCATCAATGT CACACGGAGG TTCTTGCGCT CTGTGGCCAG AGTGTTTGTC
    ATCCTCAGCG TGGAGATGGC ATCTTCCAAG AAAAAGAACA ACTTCATCCC TCAGCCTATT
    GGGAAGTGCA GGCGTGTGTT CCAGGCACTG TTGCCGTACG CAGTGGAGGA GCTCTGCAAT
    GTGGCCGAGT CTCTGATCGT GCCGGTGCGT ATGGGCATCG CTCGGCCCAC AGCTCCCTTC
    ACTCTGGCCA GTACCAGCAT TGATGCAGTG CAGGGCAGTG AGGAGCTCTT CTCTGTGGAG
    CCTCTGCCAC CTCGACCCTC CCCGGACCAG TCCAGCAGCT CCAGTCAGTC TGCCTCTTCA
    TACATCATCA GGAACCCGCA GCCCCGCCGC AGCAGCCAGT CTCAGACAGC CCGCGGACGG
    GACGAGGAGC AGGATGATAT CGTGTCTGCT GACGTAGAAG AGGTGGAGGT GGTGGAGGGT
    GTTGCAGGTG AGGAGGATCA CCATGATGAC CAGGAGGAGC AGGGAGAGGA GAACGCAGAG
    GCTGAAGGAC AGCATGATGA GCACGATGAG GATGGGAGCG ACATGGAGCT GGATTTATTG
    GCTGCCGCTG AGACCGAGAG TGACAGTGAG AGTAACCATA GCAACCAGGA CAATGCGAGT
    GGCCGCAGGA GTGTTGTGAC GGCAGCCACT GCTGGATCTG AAGCAGGTGC CAGCAGTGTT
    CCTGCTTTCT TCTCCGAGGA CGACTCCCAG TCGAATGACT CCAGCGACTC GGACAGCAGC
    AGCAGCCAGA GTGACGATGT GGATCAGGAG ACGTTTCTGC TGGACGAGCC TCTTGAGAGA
    ACCACCGGCT CAGCACATGC TAACAGTGCA GCCCAGGCCC CACGCTCCAT GCAGTGGGCT
    GTTCGCACTA CACCCAGCCA GCGCTCTGGA GGTGGCGCCC CATCCAGCTC GTCCGCTCCT
    GCTGCGAGCT CCACTGGCCT CATCTACATC GACCCGTCAA ACCTGCGCCG CAGCAGCGCC
    ATCAGCACCA GCGCAGCCGC TGCCGCAGCA GCCCTGGAGG CCAGTAACTC CAGCAGTTAT
    CTGACGTCTG CCAGCAGCCT GGCTCGAGCA TACAGCATCG TCATCCGCCA GATCTCTGAC
    CTCATGAGTC TCATTCCCAA ATACAACCAC CTGGTCTACT CGCAGTACCC CGCTGCTGTC
    AAACTCACCT ATCAGGATGC TGTCAATCTG CAGAACTTTG TGGAGGACAA GTTGATTCCC
    ACCTGGAACT GGATGGTGTC CATCATGGAC TCCACTGAAG CGCAGCTGCG TTACGGCTCG
    GCTCTGTCTT CAGCCGGGGA TCCTGGTCAT CCCAGCCACC CGCTACACGC ATCCCAGCAT
    GCCGGCCGCA GAGAGCGCAT GACCGCTCGA GAGGAGGCCA GTCTGCGCAC TCTAGAAGGA
    CGAAGGCGTG CTGCTACGCT TCTGACGGCA CGTCAGGGCA TGATGTCAGC GCGAGGAGAT
    TTCCTGAACT ACGCTCTGTC TCTGATGCGT TCCCATAATG ACGAGCATTC AGACGTTCTG
    CCTGTGTTAG ATGTCTGTTC GCTCAAACAC GTGGCTTACG TCTTCCAGGC GCTCATCTAC
    TGGATTAAAG CCATGAATCT GCAGACCACG CTCGACACCA CTCAGATTGA CAGGAAGAGG
    AATCGTGAGC TGCTGGAGCT CGGTCTGGAC AATGAAGACT CTGAGCACGA GAACGATGAG
    GACACCAATC AGAGCTCTAC ACTGCAGGAT AAGGACGAGG AGCCTGTGCC TGCAGAGACT
    GGACAACACC ATCCGTTTTT CCGCCGCTCG GACTCCATGA CCTTCCTGGG GTGCATCCCT
    CCAAACCCTT TTGAAGTTCC TCTAGCAGAG GCCATTCCTC TGGCTGACCA GCCGCATCTC
    CTGCAGCCCA ATGCCAGAAA GGAGGATTTG TTTGGACGGC CTAGTCAAGG TCTATATTCG
    TCCTCATATA TGGCCAGTAA AGGCCTCACC GATCTGACTG TGGACATGAA TTGCTTGCAG
    ATTCTACCCA CAAAGATGTC GTATTCGGCG AACATGAAGA ACGTGATGAG TATGGAGTCC
    CGGCAGAGGG GTGGCGAGGA GCAGCCGGTG GCGGAGCAGG AGATGGATGT GTCTAAACCC
    GGCCCTTCAC CACACGATCT GGCGGCCCAG TTAAAGAGCA GCCTGCTGGC AGAGATTGGC
    CTGACTGAGA GTGATGGCCC ACCGCTGCCC ACGTTTATTC CACACTGTAG TTTTATGGGG
    ATGGTGATCT CTCATGACAT GCTGCTGGGC CGCTGGCGGC TTTCTCTGGA GCTGTTTGGC
    CGTGTTTTCA TGGAGGACGT TGGAGCAGAA CCAGGATCTA TCCTGACAGA GCTGGGGGGT
    TTTGAGGTAA AGGAGTCCAA GTTTCGTCGT GAGATGGAGA AGCTGCGTAA CCTTCAGTCG
    CGTGATCTGG CCCTGGAGGT GGACCGAGAC CGTGAGCAGC TCATCCAGCA GACCATGCGG
    CAGCTGAACG CTCACTTCGG CCGCCGCTGT ACCACCACAC CCATGGCCGT GCACCGCGTA
    AAGGTGACAT TCAAGGATGA GCCTGGAGAG GGCAGTGGTG TCGCTCGCAG CTTCTACACC
    GCCATCGCTC AGGCCTTCTT GTCCAACGAC AAGCTGCCCA ATCTGGACTG CGTGCAGAGC
    GTCAGCAAGG GGATGCAGGC TAATCTTATG CAGCGGCTGA GGAACCGAGA TCGTGAGCGT
    GAGAGGAGGA GCGGAGGACT GAGAGCTGCA TCCAGACGAG ATCGTGACAG GGACTCGCGG
    AGGCAGTTGT CCATCGACAC CCGGCCGTTC AGACCTGCAT CAGAAGGAAA CCCCAGTGAT
    GAACCAGAAC CGCTGCCGGC TCACAGACAG GCTCTGGGAG AGAGACTTTA CCCACGAGTT
    CATGCCATGC AACCGGCGTT TGCTAGTAAA ATCACAGGGA TGCTGCTGGA ACTGTCTCCC
    GCTCAGCTGC TGCTACTCCT GGCCAGCGAA GACTCGCTCA GAGCCCGAGT GGAGGAGGCC
    ATGGAGCTGC TCATAACTCA TGGACGGGAA AATGGTGCTG ACAGTATACT AGACCTCGGC
    CTCCTCGACA CTCCTGAGAA AGCACAACAG GAGAACCGAA AGAGGCATGG TTCGACTCGC
    AGTGTGGTGG ACATGGAGCT GGATGACCCT GATGATGGGG ATGATAACGC TCCTCTGTTT
    TACCAGCCTG GAAAGAGGGG TTTCTACTCA CCACGTCCCG GCAAGAACAC TGAGGCCAGG
    CTCAACTGTT TCCGTAACAT CGGCAGGATA CTGGGTTTGT GTCTGCTACA GAATGAGCTT
    TGCCCAATCA CTCTCAACAG ACACGTCATC AAGGTGCTGC TCGGCAGAAA GGTGAACTGG
    CACGACTTTG CGTTCTTCGA CCCGGTAATG TACGAGAGTT TGCGGCAGCT CATCCGTCAC
    TCTCAGACTG AAGAGGCGGA GGCTGTGTTT GCGGCGATGG ACCTGGCCTT CGCTATCGAC
    CTCTGCAAGG AGGAGGGAGC AGGACAGGTG GAGCTGCTGT CCGGTGGAGT CAACATGCCG
    GTGACGCCTC TGAATGTGTA CGAGTACGTG AGGAGATACG CAGAGCACCG GATGCTGGTG
    GTGGCAGAAC AGCCTCTGCA TGCCATGCGG AAAGGTCTTC TGGATGTGCT TCCTAAGAAC
    GCTCTGGAAG ATCTGACAGC GGAGGATTTC AGACTGCTGG TCAACGGCTG TGGAGAAGTC
    AACGTCCAGA TGCTCATTAG TTTCACTTCA TTCAATGATG AGTCAGGTGA GAATGCAGAG
    AAGCTGCTGC AGTTCAAGCG CTGGTTTTGG TCCATCGTTG AGAAGATGAG CATGACGGAA
    AGGCAGGACC TGGTGTACTT CTGGACGTCC AGCCCGTCTC TGCCGGCCAG TGAAGAAGGT
    TTCCAGCCGA TGCCCTCCAT CACCATCCGC CCGCCGGACG ACCAGCACCT GCCCACAGCC
    AACACCTGCA TCTCTCGCCT CTACGTGCCA CTTTACTCTT CCAAACAGAT CCTCAAACAG
    AAACTTTTAC TAGCCATCAA GACCAAGAAC TTCGGTTTTG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001157866.1
    CDS1..8385
    Translation

    Target ORF information:

    RefSeq Version NM_001164394.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001164394.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGA
    GGAGGATCTT CAGGACGTTC ATCCACAGCA GCCCGTGATT CACGCAGGCA GACCCGCGTC
    ATCCGCACCG GCAGAGATCG AGGCTCTGGC CTGCTGGGAA GCCAACCACA GCCTGTGATT
    CCAGCCTCCG TCATCCCCGA GGAGCTCATC TCTCAGGCTC AGGTGGTTCT TCAGGGGAAA
    TCCAGAAGTG TCATCATTCG AGAGCTGCAG CGCACAAACC TTGACGTGAA CTTGGCTGTA
    AACAACCTGC TGAGCAGAGA TGATGAGGAT GGAGATGATG GCGATGACAC GGCCAGCGAA
    TCCTACTTAC CTGGAGAAGA CTTGATGTCG CTGCTAGATG CAGACATTCA CTCCGCTCAC
    CCCAGCGTGA TCATAGATGC CGATGCCATG TTCTCTGAGG ACATCAGCTA CTTCGGTTAC
    CCCTCTTTTC GTCGCTCCTC CTTGTCTCGT CTTGGCTCCT CACGAGTTCT CCTTCTCCCC
    CTAGAGAGAG ACTCTGAGCT GCTCCGCGAG CGTGAGTCGG TCCTGAGGCT GCGGGAGAGG
    CGGTGGCTGG ACGGGGCCTC CTTCGATGCG GAGCGTGGCT CCACCAGCCG CGAGGGAGAG
    CCCAGCCTTG ACAAGAAGAG TGTCCCGCTG CAGAGCCCAG TCACTCTGGG GGAAGAGCTC
    CAGTGGTGGC CTGATAAGGA CTATGTAACA AAGTTTGTCA GCATTGGTGC TTTGTACTCG
    GAGCTGGTTG CCGTCAGTAC CAAAGGAGAA CTCTACCAGT GGAAGTGGAA TGAACCTGAA
    CCTTACAGAA ACGCACAAAA TCCTTCTATA CATCACCCTC GTGTTCCTTT CCTGGGCTTG
    ACCAATGAAA AGATCACCCA CCTGTCCGCC AACAGTATCC GAGCCACTGT GGCCACCGAG
    AACAACAAGG TGGCAACATG GGTGGATGAA ACTCTGAGCA CTGTTGCTGC TAAACTCGAA
    CACGGAGCAC AAACGTTCCC GGAGCTGCAG GGGGAGCGGA TCGTCTCTCT GCACTGCTGT
    GCCCTGTATA CCTGCGCTCA GCTGGAGAAC AGCCTTTACT GGTGGGGTGT TGTGCCTTTT
    AGTCAACGGA AAAAAATGCT TGAAAAGGCC AGAGCCAAGA ACAAGAAACC AAAGTCCAGT
    GCTGGAATCT CTTCGATACC CAACATCACA GTAGGAACTC AGGTGTGCCT GAGGAATAAT
    CCTCTTTATC ACGCTGGTGC CGTGGCCTTT TCCGTGAACG CCGGGATCCC TAAAGTGGGC
    GTCCTGCTGG AGTCGGTGTG GAACATGAAC GACAGCTGCC GCTTCCAGCT GCGCTCGCCA
    GAGAGCCTGA AAAACATGGA GAAAAACACC AAGACACAGG AGACCAAGAC GGAGAGCAAG
    CCAGAGCTGG TGAAGACGGA AATGGGCCCT CCGCCCTCAC CAGCGTCCAC ATGCAGTGAC
    ACTTCATCCA TCGCTAGCAG CGCTTCACTG CCGTACAAGC GAAGGCGCTC AACACCGGCT
    CCGAAGGAGG AGGAGAAGGT GAATGAGGAG CAGTGGCCTC TGCGAGAGGT TGTTTTCGTG
    GAGGATGTTA AAAACGTCCC CGTGGGAAAG GTGCTAAAAG TCGATGGTGC ATATGTTGCT
    GTGAAATTTC CAGGGACGTC GAGCAGCGTG AGCAGCCAGA GCGCAGCTCC CACTGACTCT
    GACCCGTCCT CACTGCTGCA AGACTGTCGA CTGCTCAGAA TAGATGAGCT ACAGGTTGTT
    AAAACTGGTG GAACACCAAA AGTTCCTGAC TGTTTTCAGC GAACACCTAA AAAACTTTGC
    ATCCCAGAAA AAGCTGAAAT TTTAGCAGTG AATGTTGACT CCAAAGGAGT TCATGCTGTG
    TTGAAAACGG GCAGCTGGGT GAGATACTGT GTCTTTGACT TAGCCACCGG CAAAGCCGAG
    CAAGAGAATC ACTTTCCCAC CAGTAACCTG GCGTTTCTTG GTCAGAGCGA GCGAAATGTG
    GCCATCTTCA CTGCTGGCCA GGAGAGTCCC ATTATTTTGA GGGATGGCAA TGGCACTATT
    TATCCCATGG CTAAAGACTG TATGGGTGGA ATACGTGACC CTGATTGGCT GGATCTGCCG
    CCTATAGCCA GTCTGGGCAT GGGAGTTCAC TCGCTCGCCA ACCTTCCCAG TAACTCCACA
    ATCAAAAAGA AGGCGGCCAT CATCATAATG GCTGTAGAGA AGCAGACTCT GATGCAGCAC
    GTGCTGCGCT GTGATTACGA GGCGTGTAGG CAGTATCTGA TCAGTCTGGA GCAGGCCATG
    CTACTGGAGC AGAACCCTCA CGCTCTGGAC ACTCTGCTAG GACACCGCTG TGACGGAAAC
    CGCAACGTCC TCCATGCCTG TGTGTCCGTC TGCTTCCCCG TCAGCAACAA GGAAACCAAG
    GAGGAAGAAG AAGCAGAGCG GTCTGAAAGA AACACGTTCG CTGAGAGGCT TTCTGCAGTG
    GAGGCCATTG CCAATGCCAT ATCAGTGGTG TCGAGCAACA GCTCCGGGAA CCGGACAGGA
    TCATCCAGCA GCAGGGGCTT GCGTTTGAGA GAGATGATGC GGCGCTCACT CCGTGCTGCA
    GGTTTAGGCC GACATGAATC CGGACCTTCC TCCAGCGACC ATCAAGACCC CGTTTCACCC
    CCGATAGCAC CTCCTAGCTG GGTACCAGAC CCTCCACCGA TGGATCCTGA CGGTGACATT
    GATTTCATCT TGGCCCCTGC AGTGGGCTCC CTCACCACTG CTTCCACAGG CACCAGCCAG
    GGACCCAGCA CTTCCACCAT CCCAGGACCC TCCTCTGAAC CATCAGTAGT GGAATCTAAA
    GACCGTAAGG CGAACGCACA CCTGATACTG AAGCTGATGT GTGACAGCAT CGTCCTGCGG
    CCTCATCTAA GAGAACTGCT CTCTGCCAAG GATGCTCGTG GAATGACTCC ATTCATGTTA
    GCAGTGAGTG GCAGAGCTTA CCCAGCAGCC ATTACTGTCT TAGAAGCAGC ACAGAAAATT
    GCCAAAGGTG AACCAGGTCT TGGAGAGAAA GAGGATACAG CATCTGTGTT CATGGAGATG
    ATCTGTCCTT CAGGAACCAA TCCAGACGAC TCTCCGCTGT ACGTCCTGTG CTGTAACGAC
    ACCTGCAGCT TCACCTGGAC TGGAGCAGAA CACATCAACC AGGACATCTT TGAGTGCAGA
    ACTTGTGGCC TGCTGGAGTC GCTGTGCTGC TGCACTGAAT GTGCTAGAGT CTGTCACAAG
    GGGCACGACT GCAAACTGAA GAGGACGTCA CCAACAGCTT ACTGTGACTG CTGGGAGAAG
    TGCAAATGCA AGACTCTCAT CGCAGGCCAG AAAGCGGCAC GTCTGGATCT GCTCTACAGG
    CTGCTGACCA CCACTAATCT AGTGACCACT CCTAACAGCA GGGGAGAGCA CATCTTGCTG
    TTTCTGGTGC AGACTGTTGC AAGGCAGAGT GTCGAACACT GTCAGTATCG ACCTCCTCGC
    ATTCGAGAGG ACAGGAACCG GAAAGCTGCC AGTGCTGAAG ACTCTGATAT GCCAGATCAT
    GACCTGGAAC CTCCACGCTT TGCTCAGCTG GCTCTGGAGC GAGTGTTGCA GGACTGGAAC
    GCACTGAAGT CAATGATAAT GTTCGGCTCT CAGGAAAATA AAGATCCGTT AAGCGCTAGC
    AGCAGAATCG CTCACCTTTT ACCGGAGGAG CAGATGTACC TCAACCAGCA GAGTGGCACC
    ATCAGACTGG ACTGTTTCAC ACACTGCCTT ATTGTCAAGT GTGCACCAGA CATCACTTTC
    ATTGACACAC TGTTAGGGAC CCTGGTGAAG GAGCTGCAGA ATAAATACAC CCCCGGCCGG
    CGGGAAGAGG CCATCAATGT CACACGGAGG TTCTTGCGCT CTGTGGCCAG AGTGTTTGTC
    ATCCTCAGCG TGGAGATGGC ATCTTCCAAG AAAAAGAACA ACTTCATCCC TCAGCCTATT
    GGGAAGTGCA GGCGTGTGTT CCAGGCACTG TTGCCGTACG CAGTGGAGGA GCTCTGCAAT
    GTGGCCGAGT CTCTGATCGT GCCGGTGCGT ATGGGCATCG CTCGGCCCAC AGCTCCCTTC
    ACTCTGGCCA GTACCAGCAT TGATGCAGTG CAGGGCAGTG AGGAGCTCTT CTCTGTGGAG
    CCTCTGCCAC CTCGACCCTC CCCGGACCAG TCCAGCAGCT CCAGTCAGTC TGCCTCTTCA
    TACATCATCA GGAACCCGCA GCCCCGCCGC AGCAGCCAGT CTCAGACAGC CCGCGGACGG
    GACGAGGAGC AGGATGATAT CGTGTCTGCT GACGTAGAAG AGGTGGAGGT GGTGGAGGGT
    GTTGCAGGTG AGGAGGATCA CCATGATGAC CAGGAGGAGC AGGGAGAGGA GAACGCAGAG
    GCTGAAGGAC AGCATGATGA GCACGATGAG GATGGGAGCG ACATGGAGCT GGATTTATTG
    GCTGCCGCTG AGACCGAGAG TGACAGTGAG AGTAACCATA GCAACCAGGA CAATGCGAGT
    GGCCGCAGGA GTGTTGTGAC GGCAGCCACT GCTGGATCTG AAGCAGGTGC CAGCAGTGTT
    CCTGCTTTCT TCTCCGAGGA CGACTCCCAG TCGAATGACT CCAGCGACTC GGACAGCAGC
    AGCAGCCAGA GTGACGATGT GGATCAGGAG ACGTTTCTGC TGGACGAGCC TCTTGAGAGA
    ACCACCGGCT CAGCACATGC TAACAGTGCA GCCCAGGCCC CACGCTCCAT GCAGTGGGCT
    GTTCGCACTA CACCCAGCCA GCGCTCTGGA GGTGGCGCCC CATCCAGCTC GTCCGCTCCT
    GCTGCGAGCT CCACTGGCCT CATCTACATC GACCCGTCAA ACCTGCGCCG CAGCAGCGCC
    ATCAGCACCA GCGCAGCCGC TGCCGCAGCA GCCCTGGAGG CCAGTAACTC CAGCAGTTAT
    CTGACGTCTG CCAGCAGCCT GGCTCGAGCA TACAGCATCG TCATCCGCCA GATCTCTGAC
    CTCATGAGTC TCATTCCCAA ATACAACCAC CTGGTCTACT CGCAGTACCC CGCTGCTGTC
    AAACTCACCT ATCAGGATGC TGTCAATCTG CAGAACTTTG TGGAGGACAA GTTGATTCCC
    ACCTGGAACT GGATGGTGTC CATCATGGAC TCCACTGAAG CGCAGCTGCG TTACGGCTCG
    GCTCTGTCTT CAGCCGGGGA TCCTGGTCAT CCCAGCCACC CGCTACACGC ATCCCAGCAT
    GCCGGCCGCA GAGAGCGCAT GACCGCTCGA GAGGAGGCCA GTCTGCGCAC TCTAGAAGGA
    CGAAGGCGTG CTGCTACGCT TCTGACGGCA CGTCAGGGCA TGATGTCAGC GCGAGGAGAT
    TTCCTGAACT ACGCTCTGTC TCTGATGCGT TCCCATAATG ACGAGCATTC AGACGTTCTG
    CCTGTGTTAG ATGTCTGTTC GCTCAAACAC GTGGCTTACG TCTTCCAGGC GCTCATCTAC
    TGGATTAAAG CCATGAATCT GCAGACCACG CTCGACACCA CTCAGATTGA CAGGAAGAGG
    AATCGTGAGC TGCTGGAGCT CGGTCTGGAC AATGAAGACT CTGAGCACGA GAACGATGAG
    GACACCAATC AGAGCTCTAC ACTGCAGGAT AAGGACGAGG AGCCTGTGCC TGCAGAGACT
    GGACAACACC ATCCGTTTTT CCGCCGCTCG GACTCCATGA CCTTCCTGGG GTGCATCCCT
    CCAAACCCTT TTGAAGTTCC TCTAGCAGAG GCCATTCCTC TGGCTGACCA GCCGCATCTC
    CTGCAGCCCA ATGCCAGAAA GGAGGATTTG TTTGGACGGC CTAGTCAAGG TCTATATTCG
    TCCTCATATA TGGCCAGTAA AGGCCTCACC GATCTGACTG TGGACATGAA TTGCTTGCAG
    ATTCTACCCA CAAAGATGTC GTATTCGGCG AACATGAAGA ACGTGATGAG TATGGAGTCC
    CGGCAGAGGG GTGGCGAGGA GCAGCCGGTG GCGGAGCAGG AGATGGATGT GTCTAAACCC
    GGCCCTTCAC CACACGATCT GGCGGCCCAG TTAAAGAGCA GCCTGCTGGC AGAGATTGGC
    CTGACTGAGA GTGATGGCCC ACCGCTGCCC ACGTTTATTC CACACTGTAG TTTTATGGGG
    ATGGTGATCT CTCATGACAT GCTGCTGGGC CGCTGGCGGC TTTCTCTGGA GCTGTTTGGC
    CGTGTTTTCA TGGAGGACGT TGGAGCAGAA CCAGGATCTA TCCTGACAGA GCTGGGGGGT
    TTTGAGGTAA AGGAGTCCAA GTTTCGTCGT GAGATGGAGA AGCTGCGTAA CCTTCAGTCG
    CGTGATCTGG CCCTGGAGGT GGACCGAGAC CGTGAGCAGC TCATCCAGCA GACCATGCGG
    CAGCTGAACG CTCACTTCGG CCGCCGCTGT ACCACCACAC CCATGGCCGT GCACCGCGTA
    AAGGTGACAT TCAAGGATGA GCCTGGAGAG GGCAGTGGTG TCGCTCGCAG CTTCTACACC
    GCCATCGCTC AGGCCTTCTT GTCCAACGAC AAGCTGCCCA ATCTGGACTG CGTGCAGAGC
    GTCAGCAAGG GGATGCAGGC TAATCTTATG CAGCGGCTGA GGAACCGAGA TCGTGAGCGT
    GAGAGGAGGA GCGGAGGACT GAGAGCTGCA TCCAGACGAG ATCGTGACAG GGACTCGCGG
    AGGCAGTTGT CCATCGACAC CCGGCCGTTC AGACCTGCAT CAGAAGGAAA CCCCAGTGAT
    GAACCAGAAC CGCTGCCGGC TCACAGACAG GCTCTGGGAG AGAGACTTTA CCCACGAGTT
    CATGCCATGC AACCGGCGTT TGCTAGTAAA ATCACAGGGA TGCTGCTGGA ACTGTCTCCC
    GCTCAGCTGC TGCTACTCCT GGCCAGCGAA GACTCGCTCA GAGCCCGAGT GGAGGAGGCC
    ATGGAGCTGC TCATAACTCA TGGACGGGAA AATGGTGCTG ACAGTATACT AGACCTCGGC
    CTCCTCGACA CTCCTGAGAA AGCACAACAG GAGAACCGAA AGAGGCATGG TTCGACTCGC
    AGTGTGGTGG ACATGGAGCT GGATGACCCT GATGATGGGG ATGATAACGC TCCTCTGTTT
    TACCAGCCTG GAAAGAGGGG TTTCTACTCA CCACGTCCCG GCAAGAACAC TGAGGCCAGG
    CTCAACTGTT TCCGTAACAT CGGCAGGATA CTGGGTTTGT GTCTGCTACA GAATGAGCTT
    TGCCCAATCA CTCTCAACAG ACACGTCATC AAGGTGCTGC TCGGCAGAAA GGTGAACTGG
    CACGACTTTG CGTTCTTCGA CCCGGTAATG TACGAGAGTT TGCGGCAGCT CATCCGTCAC
    TCTCAGACTG AAGAGGCGGA GGCTGTGTTT GCGGCGATGG ACCTGGCCTT CGCTATCGAC
    CTCTGCAAGG AGGAGGGAGC AGGACAGGTG GAGCTGCTGT CCGGTGGAGT CAACATGCCG
    GTGACGCCTC TGAATGTGTA CGAGTACGTG AGGAGATACG CAGAGCACCG GATGCTGGTG
    GTGGCAGAAC AGCCTCTGCA TGCCATGCGG AAAGGTCTTC TGGATGTGCT TCCTAAGAAC
    GCTCTGGAAG ATCTGACAGC GGAGGATTTC AGACTGCTGG TCAACGGCTG TGGAGAAGTC
    AACGTCCAGA TGCTCATTAG TTTCACTTCA TTCAATGATG AGTCAGGTGA GAATGCAGAG
    AAGCTGCTGC AGTTCAAGCG CTGGTTTTGG TCCATCGTTG AGAAGATGAG CATGACGGAA
    AGGCAGGACC TGGTGTACTT CTGGACGTCC AGCCCGTCTC TGCCGGCCAG TGAAGAAGGT
    TTCCAGCCGA TGCCCTCCAT CACCATCCGC CCGCCGGACG ACCAGCACCT GCCCACAGCC
    AACACCTGCA TCTCTCGCCT CTACGTGCCA CTTTACTCTT CCAAACAGAT CCTCAAACAG
    AAACTTTTAC TAGCCATCAA GACCAAGAAC TTCGGTTTTG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149204
    Clone ID Related Accession (Same CDS sequence) XM_021466626.1
    Accession Version XM_021466626.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGC
    TCCAGTCAGT CTGCCTCTTC ATACATCATC AGGAACCCGC AGCCCCGCCG CAGCAGCCAG
    TCTCAGACAG CCCGCGGACG GGACGAGGAG CAGGATGATA TCGTGTCTGC TGACGTAGAA
    GAGGTGGAGG TGGTGGAGGG TGTTGCAGGT GAGGAGGATC ACCATGATGA CCAGGAGGAG
    CAGGGAGAGG AGAACGCAGA GGCTGAAGGA CAGCATGATG AGCACGATGA GGATGGGAGC
    GACATGGAGC TGGATTTATT GGCTGCCGCT GAGACCGAGA GTGACAGTGA GAGTAACCAT
    AGCAACCAGG ACAATGCGAG TGGCCGCAGG AGTGTTGTGA CGGCAGCCAC TGCTGGATCT
    GAAGCAGGTG CCAGCAGTGT TCCTGCTTTC TTCTCCGAGG ACGACTCCCA GTCGAATGAC
    TCCAGCGACT CGGACAGCAG CAGCAGCCAG AGTGACGATG TGGATCAGGA GACGTTTCTG
    CTGGACGAGC CTCTTGAGAG AACCACCGGC TCAGCACATG CTAACAGTGC AGCCCAGGCC
    CCACGCTCCA TGCAGTGGGC TGTTCGCACT ACACCCAGCC AGCGCTCTGG AGGTGGCGCC
    CCATCCAGCT CGTCCGCTCC TGCTGCGAGC TCCACTGGCC TCATCTACAT CGACCCGTCA
    AACCTGCGCC GCAGCAGCGC CATCAGCACC AGCGCAGCCG CTGCCGCAGC AGCCCTGGAG
    GCCAGTAACT CCAGCAGTTA TCTGACGTCT GCCAGCAGCC TGGCTCGAGC ATACAGCATC
    GTCATCCGCC AGATCTCTGA CCTCATGAGT CTCATTCCCA AATACAACCA CCTGGTCTAC
    TCGCAGTACC CCGCTGCTGT CAAACTCACC TATCAGGATG CTGTCAATCT GCAGAACTTT
    GTGGAGGACA AGTTGATTCC CACCTGGAAC TGGATGGTGT CCATCATGGA CTCCACTGAA
    GCGCAGCTGC GTTACGGCTC GGCTCTGTCT TCAGCCGGGG ATCCTGGTCA TCCCAGCCAC
    CCGCTACACG CATCCCAGCA TGCCGGCCGC AGAGAGCGCA TGACCGCTCG AGAGGAGGCC
    AGTCTGCGCA CTCTAGAAGG ACGAAGGCGT GCTGCTACGC TTCTGACGGC ACGTCAGGGC
    ATGATGTCAG CGCGAGGAGA TTTCCTGAAC TACGCTCTGT CTCTGATGCG TTCCCATAAT
    GACGAGCATT CAGACGTTCT GCCTGTGTTA GATGTCTGTT CGCTCAAACA CGTGGCTTAC
    GTCTTCCAGG CGCTCATCTA CTGGATTAAA GCCATGAATC TGCAGACCAC GCTCGACACC
    ACTCAGATTG ACAGGAAGAG GAATCGTGAG CTGCTGGAGC TCGGTCTGGA CAATGAAGAC
    TCTGAGCACG AGAACGATGA GGACACCAAT CAGAGCTCTA CACTGCAGGA TAAGGACGAG
    GAGCCTGTGC CTGCAGAGAC TGGACAACAC CATCCGTTTT TCCGCCGCTC GGACTCCATG
    ACCTTCCTGG GGTGCATCCC TCCAAACCCT TTTGAAGTTC CTCTAGCAGA GGCCATTCCT
    CTGGCTGACC AGCCGCATCT CCTGCAGCCC AATGCCAGAA AGGAGGATTT GTTTGGACGG
    CCTAGTCAAG GTCTATATTC GTCCTCATAT ATGGCCAGTA AAGGCCTCAC CGATCTGACT
    GTGGACATGA ATTGCTTGCA GATTCTACCC ACAAAGATGT CGTATTCGGC GAACATGAAG
    AACGTGATGA GTATGGAGTC CCGGCAGAGG GGTGGCGAGG AGCAGCCGGT GGCGGAGCAG
    GAGATGGATG TGTCTAAACC CGGCCCTTCA CCACACGATC TGGCGGCCCA GTTAAAGAGC
    AGCCTGCTGG CAGAGATTGG CCTGACTGAG AGTGATGGCC CACCGCTGCC CACGTTTATT
    CCACACTGTA GTTTTATGGG GATGGTGATC TCTCATGACA TGCTGCTGGG CCGCTGGCGG
    CTTTCTCTGG AGCTGTTTGG CCGTGTTTTC ATGGAGGACG TTGGAGCAGA ACCAGGATCT
    ATCCTGACAG AGCTGGGGGG TTTTGAGGTA AAGGAGTCCA AGTTTCGTCG TGAGATGGAG
    AAGCTGCGTA ACCTTCAGTC GCGTGATCTG GCCCTGGAGG TGGACCGAGA CCGTGAGCAG
    CTCATCCAGC AGACCATGCG GCAGCTGAAC GCTCACTTCG GCCGCCGCTG TACCACCACA
    CCCATGGCCG TGCACCGCGT AAAGGTGACA TTCAAGGATG AGCCTGGAGA GGGCAGTGGT
    GTCGCTCGCA GCTTCTACAC CGCCATCGCT CAGGCCTTCT TGTCCAACGA CAAGCTGCCC
    AATCTGGACT GCGTGCAGAG CGTCAGCAAG GGGATGCAGG CTAGCAATCT TATGCAGCGG
    CTGAGGAACC GAGATCGTGA GCGTGAGAGG AGGAGCGGAG GACTGAGAGC TGCATCCAGA
    CGAGATCGTG ACAGCCTAAA GAGACTTTTT GCTGTGTTTG ACTGCAGGGA CTCGCGGAGG
    CAGTTGTCCA TCGACACCCG GCCGTTCAGA CCTGCATCAG AAGGAAACCC CAGTGATGAA
    CCAGAACCGC TGCCGGCTCA CAGACAGGCT CTGGGAGAGA GACTTTACCC ACGAGTTCAT
    GCCATGCAAC CGGCGTTTGC TAGTAAAATC ACAGGGATGC TGCTGGAACT GTCTCCCGCT
    CAGCTGCTGC TACTCCTGGC CAGCGAAGAC TCGCTCAGAG CCCGAGTGGA GGAGGCCATG
    GAGCTGCTCA TAACTCATGG ACGGGAAAAT GGTGCTGACA GTATACTAGA CCTCGGCCTC
    CTCGACACTC CTGAGAAAGC ACAACAGGAG AACCGAAAGA GGCATGGTTC GACTCGCAGT
    GTGGTGGACA TGGAGCTGGA TGACCCTGAT GATGGGGATG ATAACGCTCC TCTGTTTTAC
    CAGCCTGGAA AGAGGGGTTT CTACTCACCA CGTCCCGGCA AGAACACTGA GGCCAGGCTC
    AACTGTTTCC GTAACATCGG CAGGATACTG GGTTTGTGTC TGCTACAGAA TGAGCTTTGC
    CCAATCACTC TCAACAGACA CGTCATCAAG GTGCTGCTCG GCAGAAAGGT GAACTGGCAC
    GACTTTGCGT TCTTCGACCC GGTAATGTAC GAGAGTTTGC GGCAGCTCAT CCGTCACTCT
    CAGACTGAAG AGGCGGAGGC TGTGTTTGCG GCGATGGACC TGGCCTTCGC TATCGACCTC
    TGCAAGGAGG AGGGAGCAGG ACAGGTGGAG CTGCTGTCCG GTGGAGTCAA CATGCCGGTG
    ACGCCTCTGA ATGTGTACGA GTACGTGAGG AGATACGCAG AGCACCGGAT GCTGGTGGTG
    GCAGAACAGC CTCTGCATGC CATGCGGAAA GGTCTTCTGG ATGTGCTTCC TAAGAACGCT
    CTGGAAGATC TGACAGCGGA GGATTTCAGA CTGCTGGTCA ACGGCTGTGG AGAAGTCAAC
    GTCCAGATGC TCATTAGTTT CACTTCATTC AATGATGAGT CAGGTGAGAA TGCAGAGAAG
    CTGCTGCAGT TCAAGCGCTG GTTTTGGTCC ATCGTTGAGA AGATGAGCAT GACGGAAAGG
    CAGGACCTGG TGTACTTCTG GACGTCCAGC CCGTCTCTGC CGGCCAGTGA AGAAGGTTTC
    CAGCCGATGC CCTCCATCAC CATCCGCCCG CCGGACGACC AGCACCTGCC CACAGCCAAC
    ACCTGCATCT CTCGCCTCTA CGTGCCACTT TACTCTTCCA AACAGATCCT CAAACAGAAA
    CTTTTACTAG CCATCAAGAC CAAGAACTTC GGTTTTGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322301.1
    CDS173..8674
    Translation

    Target ORF information:

    RefSeq Version XM_021466626.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466626.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGC
    TCCAGTCAGT CTGCCTCTTC ATACATCATC AGGAACCCGC AGCCCCGCCG CAGCAGCCAG
    TCTCAGACAG CCCGCGGACG GGACGAGGAG CAGGATGATA TCGTGTCTGC TGACGTAGAA
    GAGGTGGAGG TGGTGGAGGG TGTTGCAGGT GAGGAGGATC ACCATGATGA CCAGGAGGAG
    CAGGGAGAGG AGAACGCAGA GGCTGAAGGA CAGCATGATG AGCACGATGA GGATGGGAGC
    GACATGGAGC TGGATTTATT GGCTGCCGCT GAGACCGAGA GTGACAGTGA GAGTAACCAT
    AGCAACCAGG ACAATGCGAG TGGCCGCAGG AGTGTTGTGA CGGCAGCCAC TGCTGGATCT
    GAAGCAGGTG CCAGCAGTGT TCCTGCTTTC TTCTCCGAGG ACGACTCCCA GTCGAATGAC
    TCCAGCGACT CGGACAGCAG CAGCAGCCAG AGTGACGATG TGGATCAGGA GACGTTTCTG
    CTGGACGAGC CTCTTGAGAG AACCACCGGC TCAGCACATG CTAACAGTGC AGCCCAGGCC
    CCACGCTCCA TGCAGTGGGC TGTTCGCACT ACACCCAGCC AGCGCTCTGG AGGTGGCGCC
    CCATCCAGCT CGTCCGCTCC TGCTGCGAGC TCCACTGGCC TCATCTACAT CGACCCGTCA
    AACCTGCGCC GCAGCAGCGC CATCAGCACC AGCGCAGCCG CTGCCGCAGC AGCCCTGGAG
    GCCAGTAACT CCAGCAGTTA TCTGACGTCT GCCAGCAGCC TGGCTCGAGC ATACAGCATC
    GTCATCCGCC AGATCTCTGA CCTCATGAGT CTCATTCCCA AATACAACCA CCTGGTCTAC
    TCGCAGTACC CCGCTGCTGT CAAACTCACC TATCAGGATG CTGTCAATCT GCAGAACTTT
    GTGGAGGACA AGTTGATTCC CACCTGGAAC TGGATGGTGT CCATCATGGA CTCCACTGAA
    GCGCAGCTGC GTTACGGCTC GGCTCTGTCT TCAGCCGGGG ATCCTGGTCA TCCCAGCCAC
    CCGCTACACG CATCCCAGCA TGCCGGCCGC AGAGAGCGCA TGACCGCTCG AGAGGAGGCC
    AGTCTGCGCA CTCTAGAAGG ACGAAGGCGT GCTGCTACGC TTCTGACGGC ACGTCAGGGC
    ATGATGTCAG CGCGAGGAGA TTTCCTGAAC TACGCTCTGT CTCTGATGCG TTCCCATAAT
    GACGAGCATT CAGACGTTCT GCCTGTGTTA GATGTCTGTT CGCTCAAACA CGTGGCTTAC
    GTCTTCCAGG CGCTCATCTA CTGGATTAAA GCCATGAATC TGCAGACCAC GCTCGACACC
    ACTCAGATTG ACAGGAAGAG GAATCGTGAG CTGCTGGAGC TCGGTCTGGA CAATGAAGAC
    TCTGAGCACG AGAACGATGA GGACACCAAT CAGAGCTCTA CACTGCAGGA TAAGGACGAG
    GAGCCTGTGC CTGCAGAGAC TGGACAACAC CATCCGTTTT TCCGCCGCTC GGACTCCATG
    ACCTTCCTGG GGTGCATCCC TCCAAACCCT TTTGAAGTTC CTCTAGCAGA GGCCATTCCT
    CTGGCTGACC AGCCGCATCT CCTGCAGCCC AATGCCAGAA AGGAGGATTT GTTTGGACGG
    CCTAGTCAAG GTCTATATTC GTCCTCATAT ATGGCCAGTA AAGGCCTCAC CGATCTGACT
    GTGGACATGA ATTGCTTGCA GATTCTACCC ACAAAGATGT CGTATTCGGC GAACATGAAG
    AACGTGATGA GTATGGAGTC CCGGCAGAGG GGTGGCGAGG AGCAGCCGGT GGCGGAGCAG
    GAGATGGATG TGTCTAAACC CGGCCCTTCA CCACACGATC TGGCGGCCCA GTTAAAGAGC
    AGCCTGCTGG CAGAGATTGG CCTGACTGAG AGTGATGGCC CACCGCTGCC CACGTTTATT
    CCACACTGTA GTTTTATGGG GATGGTGATC TCTCATGACA TGCTGCTGGG CCGCTGGCGG
    CTTTCTCTGG AGCTGTTTGG CCGTGTTTTC ATGGAGGACG TTGGAGCAGA ACCAGGATCT
    ATCCTGACAG AGCTGGGGGG TTTTGAGGTA AAGGAGTCCA AGTTTCGTCG TGAGATGGAG
    AAGCTGCGTA ACCTTCAGTC GCGTGATCTG GCCCTGGAGG TGGACCGAGA CCGTGAGCAG
    CTCATCCAGC AGACCATGCG GCAGCTGAAC GCTCACTTCG GCCGCCGCTG TACCACCACA
    CCCATGGCCG TGCACCGCGT AAAGGTGACA TTCAAGGATG AGCCTGGAGA GGGCAGTGGT
    GTCGCTCGCA GCTTCTACAC CGCCATCGCT CAGGCCTTCT TGTCCAACGA CAAGCTGCCC
    AATCTGGACT GCGTGCAGAG CGTCAGCAAG GGGATGCAGG CTAGCAATCT TATGCAGCGG
    CTGAGGAACC GAGATCGTGA GCGTGAGAGG AGGAGCGGAG GACTGAGAGC TGCATCCAGA
    CGAGATCGTG ACAGCCTAAA GAGACTTTTT GCTGTGTTTG ACTGCAGGGA CTCGCGGAGG
    CAGTTGTCCA TCGACACCCG GCCGTTCAGA CCTGCATCAG AAGGAAACCC CAGTGATGAA
    CCAGAACCGC TGCCGGCTCA CAGACAGGCT CTGGGAGAGA GACTTTACCC ACGAGTTCAT
    GCCATGCAAC CGGCGTTTGC TAGTAAAATC ACAGGGATGC TGCTGGAACT GTCTCCCGCT
    CAGCTGCTGC TACTCCTGGC CAGCGAAGAC TCGCTCAGAG CCCGAGTGGA GGAGGCCATG
    GAGCTGCTCA TAACTCATGG ACGGGAAAAT GGTGCTGACA GTATACTAGA CCTCGGCCTC
    CTCGACACTC CTGAGAAAGC ACAACAGGAG AACCGAAAGA GGCATGGTTC GACTCGCAGT
    GTGGTGGACA TGGAGCTGGA TGACCCTGAT GATGGGGATG ATAACGCTCC TCTGTTTTAC
    CAGCCTGGAA AGAGGGGTTT CTACTCACCA CGTCCCGGCA AGAACACTGA GGCCAGGCTC
    AACTGTTTCC GTAACATCGG CAGGATACTG GGTTTGTGTC TGCTACAGAA TGAGCTTTGC
    CCAATCACTC TCAACAGACA CGTCATCAAG GTGCTGCTCG GCAGAAAGGT GAACTGGCAC
    GACTTTGCGT TCTTCGACCC GGTAATGTAC GAGAGTTTGC GGCAGCTCAT CCGTCACTCT
    CAGACTGAAG AGGCGGAGGC TGTGTTTGCG GCGATGGACC TGGCCTTCGC TATCGACCTC
    TGCAAGGAGG AGGGAGCAGG ACAGGTGGAG CTGCTGTCCG GTGGAGTCAA CATGCCGGTG
    ACGCCTCTGA ATGTGTACGA GTACGTGAGG AGATACGCAG AGCACCGGAT GCTGGTGGTG
    GCAGAACAGC CTCTGCATGC CATGCGGAAA GGTCTTCTGG ATGTGCTTCC TAAGAACGCT
    CTGGAAGATC TGACAGCGGA GGATTTCAGA CTGCTGGTCA ACGGCTGTGG AGAAGTCAAC
    GTCCAGATGC TCATTAGTTT CACTTCATTC AATGATGAGT CAGGTGAGAA TGCAGAGAAG
    CTGCTGCAGT TCAAGCGCTG GTTTTGGTCC ATCGTTGAGA AGATGAGCAT GACGGAAAGG
    CAGGACCTGG TGTACTTCTG GACGTCCAGC CCGTCTCTGC CGGCCAGTGA AGAAGGTTTC
    CAGCCGATGC CCTCCATCAC CATCCGCCCG CCGGACGACC AGCACCTGCC CACAGCCAAC
    ACCTGCATCT CTCGCCTCTA CGTGCCACTT TACTCTTCCA AACAGATCCT CAAACAGAAA
    CTTTTACTAG CCATCAAGAC CAAGAACTTC GGTTTTGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149205
    Clone ID Related Accession (Same CDS sequence) XM_021466627.1
    Accession Version XM_021466627.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8499bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GCAGAGCGGT CTGAAAGAAA CACGTTCGCT
    GAGAGGCTTT CTGCAGTGGA GGCCATTGCC AATGCCATAT CAGTGGTGTC GAGCAACAGC
    TCCGGGAACC GGACAGGATC ATCCAGCAGC AGGGGCTTGC GTTTGAGAGA GATGATGCGG
    CGCTCACTCC GTGCTGCAGG TTTAGGCCGA CATGAATCCG GACCTTCCTC CAGCGACCAT
    CAAGACCCCG TTTCACCCCC GATAGCACCT CCTAGCTGGG TACCAGACCC TCCACCGATG
    GATCCTGACG GTGACATTGA TTTCATCTTG GCCCCTGCAG TGGGCTCCCT CACCACTGCT
    TCCACAGGCA CCAGCCAGGG ACCCAGCACT TCCACCATCC CAGGACCCTC CTCTGAACCA
    TCAGTAGTGG AATCTAAAGA CCGTAAGGCG AACGCACACC TGATACTGAA GCTGATGTGT
    GACAGCATCG TCCTGCGGCC TCATCTAAGA GAACTGCTCT CTGCCAAGGA TGCTCGTGGA
    ATGACTCCAT TCATGTTAGC AGTGAGTGGC AGAGCTTACC CAGCAGCCAT TACTGTCTTA
    GAAGCAGCAC AGAAAATTGC CAAAGGTGAA CCAGGTCTTG GAGAGAAAGA GGATACAGCA
    TCTGTGTTCA TGGAGATGAT CTGTCCTTCA GGAACCAATC CAGACGACTC TCCGCTGTAC
    GTCCTGTGCT GTAACGACAC CTGCAGCTTC ACCTGGACTG GAGCAGAACA CATCAACCAG
    GACATCTTTG AGTGCAGAAC TTGTGGCCTG CTGGAGTCGC TGTGCTGCTG CACTGAATGT
    GCTAGAGTCT GTCACAAGGG GCACGACTGC AAACTGAAGA GGACGTCACC AACAGCTTAC
    TGTGACTGCT GGGAGAAGTG CAAATGCAAG ACTCTCATCG CAGGCCAGAA AGCGGCACGT
    CTGGATCTGC TCTACAGGCT GCTGACCACC ACTAATCTAG TGACCACTCC TAACAGCAGG
    GGAGAGCACA TCTTGCTGTT TCTGGTGCAG ACTGTTGCAA GGCAGAGTGT CGAACACTGT
    CAGTATCGAC CTCCTCGCAT TCGAGAGGAC AGGAACCGGA AAGCTGCCAG TGCTGAAGAC
    TCTGATATGC CAGATCATGA CCTGGAACCT CCACGCTTTG CTCAGCTGGC TCTGGAGCGA
    GTGTTGCAGG ACTGGAACGC ACTGAAGTCA ATGATAATGT TCGGCTCTCA GGAAAATAAA
    GATCCGTTAA GCGCTAGCAG CAGAATCGCT CACCTTTTAC CGGAGGAGCA GATGTACCTC
    AACCAGCAGA GTGGCACCAT CAGACTGGAC TGTTTCACAC ACTGCCTTAT TGTCAAGTGT
    GCACCAGACA TCACTTTCAT TGACACACTG TTAGGGACCC TGGTGAAGGA GCTGCAGAAT
    AAATACACCC CCGGCCGGCG GGAAGAGGCC ATCAATGTCA CACGGAGGTT CTTGCGCTCT
    GTGGCCAGAG TGTTTGTCAT CCTCAGCGTG GAGATGGCAT CTTCCAAGAA AAAGAACAAC
    TTCATCCCTC AGCCTATTGG GAAGTGCAGG CGTGTGTTCC AGGCACTGTT GCCGTACGCA
    GTGGAGGAGC TCTGCAATGT GGCCGAGTCT CTGATCGTGC CGGTGCGTAT GGGCATCGCT
    CGGCCCACAG CTCCCTTCAC TCTGGCCAGT ACCAGCATTG ATGCAGTGCA GGGCAGTGAG
    GAGCTCTTCT CTGTGGAGCC TCTGCCACCT CGACCCTCCC CGGACCAGTC CAGCAGCTCC
    AGTCAGTCTG CCTCTTCATA CATCATCAGG AACCCGCAGC CCCGCCGCAG CAGCCAGTCT
    CAGACAGCCC GCGGACGGGA CGAGGAGCAG GATGATATCG TGTCTGCTGA CGTAGAAGAG
    GTGGAGGTGG TGGAGGGTGT TGCAGGTGAG GAGGATCACC ATGATGACCA GGAGGAGCAG
    GGAGAGGAGA ACGCAGAGGC TGAAGGACAG CATGATGAGC ACGATGAGGA TGGGAGCGAC
    ATGGAGCTGG ATTTATTGGC TGCCGCTGAG ACCGAGAGTG ACAGTGAGAG TAACCATAGC
    AACCAGGACA ATGCGAGTGG CCGCAGGAGT GTTGTGACGG CAGCCACTGC TGGATCTGAA
    GCAGGTGCCA GCAGTGTTCC TGCTTTCTTC TCCGAGGACG ACTCCCAGTC GAATGACTCC
    AGCGACTCGG ACAGCAGCAG CAGCCAGAGT GACGATGTGG ATCAGGAGAC GTTTCTGCTG
    GACGAGCCTC TTGAGAGAAC CACCGGCTCA GCACATGCTA ACAGTGCAGC CCAGGCCCCA
    CGCTCCATGC AGTGGGCTGT TCGCACTACA CCCAGCCAGC GCTCTGGAGG TGGCGCCCCA
    TCCAGCTCGT CCGCTCCTGC TGCGAGCTCC ACTGGCCTCA TCTACATCGA CCCGTCAAAC
    CTGCGCCGCA GCAGCGCCAT CAGCACCAGC GCAGCCGCTG CCGCAGCAGC CCTGGAGGCC
    AGTAACTCCA GCAGTTATCT GACGTCTGCC AGCAGCCTGG CTCGAGCATA CAGCATCGTC
    ATCCGCCAGA TCTCTGACCT CATGAGTCTC ATTCCCAAAT ACAACCACCT GGTCTACTCG
    CAGTACCCCG CTGCTGTCAA ACTCACCTAT CAGGATGCTG TCAATCTGCA GAACTTTGTG
    GAGGACAAGT TGATTCCCAC CTGGAACTGG ATGGTGTCCA TCATGGACTC CACTGAAGCG
    CAGCTGCGTT ACGGCTCGGC TCTGTCTTCA GCCGGGGATC CTGGTCATCC CAGCCACCCG
    CTACACGCAT CCCAGCATGC CGGCCGCAGA GAGCGCATGA CCGCTCGAGA GGAGGCCAGT
    CTGCGCACTC TAGAAGGACG AAGGCGTGCT GCTACGCTTC TGACGGCACG TCAGGGCATG
    ATGTCAGCGC GAGGAGATTT CCTGAACTAC GCTCTGTCTC TGATGCGTTC CCATAATGAC
    GAGCATTCAG ACGTTCTGCC TGTGTTAGAT GTCTGTTCGC TCAAACACGT GGCTTACGTC
    TTCCAGGCGC TCATCTACTG GATTAAAGCC ATGAATCTGC AGACCACGCT CGACACCACT
    CAGATTGACA GGAAGAGGAA TCGTGAGCTG CTGGAGCTCG GTCTGGACAA TGAAGACTCT
    GAGCACGAGA ACGATGAGGA CACCAATCAG AGCTCTACAC TGCAGGATAA GGACGAGGAG
    CCTGTGCCTG CAGAGACTGG ACAACACCAT CCGTTTTTCC GCCGCTCGGA CTCCATGACC
    TTCCTGGGGT GCATCCCTCC AAACCCTTTT GAAGTTCCTC TAGCAGAGGC CATTCCTCTG
    GCTGACCAGC CGCATCTCCT GCAGCCCAAT GCCAGAAAGG AGGATTTGTT TGGACGGCCT
    AGTCAAGGTC TATATTCGTC CTCATATATG GCCAGTAAAG GCCTCACCGA TCTGACTGTG
    GACATGAATT GCTTGCAGAT TCTACCCACA AAGATGTCGT ATTCGGCGAA CATGAAGAAC
    GTGATGAGTA TGGAGTCCCG GCAGAGGGGT GGCGAGGAGC AGCCGGTGGC GGAGCAGGAG
    ATGGATGTGT CTAAACCCGG CCCTTCACCA CACGATCTGG CGGCCCAGTT AAAGAGCAGC
    CTGCTGGCAG AGATTGGCCT GACTGAGAGT GATGGCCCAC CGCTGCCCAC GTTTATTCCA
    CACTGTAGTT TTATGGGGAT GGTGATCTCT CATGACATGC TGCTGGGCCG CTGGCGGCTT
    TCTCTGGAGC TGTTTGGCCG TGTTTTCATG GAGGACGTTG GAGCAGAACC AGGATCTATC
    CTGACAGAGC TGGGGGGTTT TGAGGTAAAG GAGTCCAAGT TTCGTCGTGA GATGGAGAAG
    CTGCGTAACC TTCAGTCGCG TGATCTGGCC CTGGAGGTGG ACCGAGACCG TGAGCAGCTC
    ATCCAGCAGA CCATGCGGCA GCTGAACGCT CACTTCGGCC GCCGCTGTAC CACCACACCC
    ATGGCCGTGC ACCGCGTAAA GGTGACATTC AAGGATGAGC CTGGAGAGGG CAGTGGTGTC
    GCTCGCAGCT TCTACACCGC CATCGCTCAG GCCTTCTTGT CCAACGACAA GCTGCCCAAT
    CTGGACTGCG TGCAGAGCGT CAGCAAGGGG ATGCAGGCTA GCAATCTTAT GCAGCGGCTG
    AGGAACCGAG ATCGTGAGCG TGAGAGGAGG AGCGGAGGAC TGAGAGCTGC ATCCAGACGA
    GATCGTGACA GCCTAAAGAG ACTTTTTGCT GTGTTTGACT GCAGGGACTC GCGGAGGCAG
    TTGTCCATCG ACACCCGGCC GTTCAGACCT GCATCAGAAG GAAACCCCAG TGATGAACCA
    GAACCGCTGC CGGCTCACAG ACAGGCTCTG GGAGAGAGAC TTTACCCACG AGTTCATGCC
    ATGCAACCGG CGTTTGCTAG TAAAATCACA GGGATGCTGC TGGAACTGTC TCCCGCTCAG
    CTGCTGCTAC TCCTGGCCAG CGAAGACTCG CTCAGAGCCC GAGTGGAGGA GGCCATGGAG
    CTGCTCATAA CTCATGGACG GGAAAATGGT GCTGACAGTA TACTAGACCT CGGCCTCCTC
    GACACTCCTG AGAAAGCACA ACAGGAGAAC CGAAAGAGGC ATGGTTCGAC TCGCAGTGTG
    GTGGACATGG AGCTGGATGA CCCTGATGAT GGGGATGATA ACGCTCCTCT GTTTTACCAG
    CCTGGAAAGA GGGGTTTCTA CTCACCACGT CCCGGCAAGA ACACTGAGGC CAGGCTCAAC
    TGTTTCCGTA ACATCGGCAG GATACTGGGT TTGTGTCTGC TACAGAATGA GCTTTGCCCA
    ATCACTCTCA ACAGACACGT CATCAAGGTG CTGCTCGGCA GAAAGGTGAA CTGGCACGAC
    TTTGCGTTCT TCGACCCGGT AATGTACGAG AGTTTGCGGC AGCTCATCCG TCACTCTCAG
    ACTGAAGAGG CGGAGGCTGT GTTTGCGGCG ATGGACCTGG CCTTCGCTAT CGACCTCTGC
    AAGGAGGAGG GAGCAGGACA GGTGGAGCTG CTGTCCGGTG GAGTCAACAT GCCGGTGACG
    CCTCTGAATG TGTACGAGTA CGTGAGGAGA TACGCAGAGC ACCGGATGCT GGTGGTGGCA
    GAACAGCCTC TGCATGCCAT GCGGAAAGGT CTTCTGGATG TGCTTCCTAA GAACGCTCTG
    GAAGATCTGA CAGCGGAGGA TTTCAGACTG CTGGTCAACG GCTGTGGAGA AGTCAACGTC
    CAGATGCTCA TTAGTTTCAC TTCATTCAAT GATGAGTCAG GTGAGAATGC AGAGAAGCTG
    CTGCAGTTCA AGCGCTGGTT TTGGTCCATC GTTGAGAAGA TGAGCATGAC GGAAAGGCAG
    GACCTGGTGT ACTTCTGGAC GTCCAGCCCG TCTCTGCCGG CCAGTGAAGA AGGTTTCCAG
    CCGATGCCCT CCATCACCAT CCGCCCGCCG GACGACCAGC ACCTGCCCAC AGCCAACACC
    TGCATCTCTC GCCTCTACGT GCCACTTTAC TCTTCCAAAC AGATCCTCAA ACAGAAACTT
    TTACTAGCCA TCAAGACCAA GAACTTCGGT TTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322302.1
    CDS173..8671
    Translation

    Target ORF information:

    RefSeq Version XM_021466627.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466627.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GCAGAGCGGT CTGAAAGAAA CACGTTCGCT
    GAGAGGCTTT CTGCAGTGGA GGCCATTGCC AATGCCATAT CAGTGGTGTC GAGCAACAGC
    TCCGGGAACC GGACAGGATC ATCCAGCAGC AGGGGCTTGC GTTTGAGAGA GATGATGCGG
    CGCTCACTCC GTGCTGCAGG TTTAGGCCGA CATGAATCCG GACCTTCCTC CAGCGACCAT
    CAAGACCCCG TTTCACCCCC GATAGCACCT CCTAGCTGGG TACCAGACCC TCCACCGATG
    GATCCTGACG GTGACATTGA TTTCATCTTG GCCCCTGCAG TGGGCTCCCT CACCACTGCT
    TCCACAGGCA CCAGCCAGGG ACCCAGCACT TCCACCATCC CAGGACCCTC CTCTGAACCA
    TCAGTAGTGG AATCTAAAGA CCGTAAGGCG AACGCACACC TGATACTGAA GCTGATGTGT
    GACAGCATCG TCCTGCGGCC TCATCTAAGA GAACTGCTCT CTGCCAAGGA TGCTCGTGGA
    ATGACTCCAT TCATGTTAGC AGTGAGTGGC AGAGCTTACC CAGCAGCCAT TACTGTCTTA
    GAAGCAGCAC AGAAAATTGC CAAAGGTGAA CCAGGTCTTG GAGAGAAAGA GGATACAGCA
    TCTGTGTTCA TGGAGATGAT CTGTCCTTCA GGAACCAATC CAGACGACTC TCCGCTGTAC
    GTCCTGTGCT GTAACGACAC CTGCAGCTTC ACCTGGACTG GAGCAGAACA CATCAACCAG
    GACATCTTTG AGTGCAGAAC TTGTGGCCTG CTGGAGTCGC TGTGCTGCTG CACTGAATGT
    GCTAGAGTCT GTCACAAGGG GCACGACTGC AAACTGAAGA GGACGTCACC AACAGCTTAC
    TGTGACTGCT GGGAGAAGTG CAAATGCAAG ACTCTCATCG CAGGCCAGAA AGCGGCACGT
    CTGGATCTGC TCTACAGGCT GCTGACCACC ACTAATCTAG TGACCACTCC TAACAGCAGG
    GGAGAGCACA TCTTGCTGTT TCTGGTGCAG ACTGTTGCAA GGCAGAGTGT CGAACACTGT
    CAGTATCGAC CTCCTCGCAT TCGAGAGGAC AGGAACCGGA AAGCTGCCAG TGCTGAAGAC
    TCTGATATGC CAGATCATGA CCTGGAACCT CCACGCTTTG CTCAGCTGGC TCTGGAGCGA
    GTGTTGCAGG ACTGGAACGC ACTGAAGTCA ATGATAATGT TCGGCTCTCA GGAAAATAAA
    GATCCGTTAA GCGCTAGCAG CAGAATCGCT CACCTTTTAC CGGAGGAGCA GATGTACCTC
    AACCAGCAGA GTGGCACCAT CAGACTGGAC TGTTTCACAC ACTGCCTTAT TGTCAAGTGT
    GCACCAGACA TCACTTTCAT TGACACACTG TTAGGGACCC TGGTGAAGGA GCTGCAGAAT
    AAATACACCC CCGGCCGGCG GGAAGAGGCC ATCAATGTCA CACGGAGGTT CTTGCGCTCT
    GTGGCCAGAG TGTTTGTCAT CCTCAGCGTG GAGATGGCAT CTTCCAAGAA AAAGAACAAC
    TTCATCCCTC AGCCTATTGG GAAGTGCAGG CGTGTGTTCC AGGCACTGTT GCCGTACGCA
    GTGGAGGAGC TCTGCAATGT GGCCGAGTCT CTGATCGTGC CGGTGCGTAT GGGCATCGCT
    CGGCCCACAG CTCCCTTCAC TCTGGCCAGT ACCAGCATTG ATGCAGTGCA GGGCAGTGAG
    GAGCTCTTCT CTGTGGAGCC TCTGCCACCT CGACCCTCCC CGGACCAGTC CAGCAGCTCC
    AGTCAGTCTG CCTCTTCATA CATCATCAGG AACCCGCAGC CCCGCCGCAG CAGCCAGTCT
    CAGACAGCCC GCGGACGGGA CGAGGAGCAG GATGATATCG TGTCTGCTGA CGTAGAAGAG
    GTGGAGGTGG TGGAGGGTGT TGCAGGTGAG GAGGATCACC ATGATGACCA GGAGGAGCAG
    GGAGAGGAGA ACGCAGAGGC TGAAGGACAG CATGATGAGC ACGATGAGGA TGGGAGCGAC
    ATGGAGCTGG ATTTATTGGC TGCCGCTGAG ACCGAGAGTG ACAGTGAGAG TAACCATAGC
    AACCAGGACA ATGCGAGTGG CCGCAGGAGT GTTGTGACGG CAGCCACTGC TGGATCTGAA
    GCAGGTGCCA GCAGTGTTCC TGCTTTCTTC TCCGAGGACG ACTCCCAGTC GAATGACTCC
    AGCGACTCGG ACAGCAGCAG CAGCCAGAGT GACGATGTGG ATCAGGAGAC GTTTCTGCTG
    GACGAGCCTC TTGAGAGAAC CACCGGCTCA GCACATGCTA ACAGTGCAGC CCAGGCCCCA
    CGCTCCATGC AGTGGGCTGT TCGCACTACA CCCAGCCAGC GCTCTGGAGG TGGCGCCCCA
    TCCAGCTCGT CCGCTCCTGC TGCGAGCTCC ACTGGCCTCA TCTACATCGA CCCGTCAAAC
    CTGCGCCGCA GCAGCGCCAT CAGCACCAGC GCAGCCGCTG CCGCAGCAGC CCTGGAGGCC
    AGTAACTCCA GCAGTTATCT GACGTCTGCC AGCAGCCTGG CTCGAGCATA CAGCATCGTC
    ATCCGCCAGA TCTCTGACCT CATGAGTCTC ATTCCCAAAT ACAACCACCT GGTCTACTCG
    CAGTACCCCG CTGCTGTCAA ACTCACCTAT CAGGATGCTG TCAATCTGCA GAACTTTGTG
    GAGGACAAGT TGATTCCCAC CTGGAACTGG ATGGTGTCCA TCATGGACTC CACTGAAGCG
    CAGCTGCGTT ACGGCTCGGC TCTGTCTTCA GCCGGGGATC CTGGTCATCC CAGCCACCCG
    CTACACGCAT CCCAGCATGC CGGCCGCAGA GAGCGCATGA CCGCTCGAGA GGAGGCCAGT
    CTGCGCACTC TAGAAGGACG AAGGCGTGCT GCTACGCTTC TGACGGCACG TCAGGGCATG
    ATGTCAGCGC GAGGAGATTT CCTGAACTAC GCTCTGTCTC TGATGCGTTC CCATAATGAC
    GAGCATTCAG ACGTTCTGCC TGTGTTAGAT GTCTGTTCGC TCAAACACGT GGCTTACGTC
    TTCCAGGCGC TCATCTACTG GATTAAAGCC ATGAATCTGC AGACCACGCT CGACACCACT
    CAGATTGACA GGAAGAGGAA TCGTGAGCTG CTGGAGCTCG GTCTGGACAA TGAAGACTCT
    GAGCACGAGA ACGATGAGGA CACCAATCAG AGCTCTACAC TGCAGGATAA GGACGAGGAG
    CCTGTGCCTG CAGAGACTGG ACAACACCAT CCGTTTTTCC GCCGCTCGGA CTCCATGACC
    TTCCTGGGGT GCATCCCTCC AAACCCTTTT GAAGTTCCTC TAGCAGAGGC CATTCCTCTG
    GCTGACCAGC CGCATCTCCT GCAGCCCAAT GCCAGAAAGG AGGATTTGTT TGGACGGCCT
    AGTCAAGGTC TATATTCGTC CTCATATATG GCCAGTAAAG GCCTCACCGA TCTGACTGTG
    GACATGAATT GCTTGCAGAT TCTACCCACA AAGATGTCGT ATTCGGCGAA CATGAAGAAC
    GTGATGAGTA TGGAGTCCCG GCAGAGGGGT GGCGAGGAGC AGCCGGTGGC GGAGCAGGAG
    ATGGATGTGT CTAAACCCGG CCCTTCACCA CACGATCTGG CGGCCCAGTT AAAGAGCAGC
    CTGCTGGCAG AGATTGGCCT GACTGAGAGT GATGGCCCAC CGCTGCCCAC GTTTATTCCA
    CACTGTAGTT TTATGGGGAT GGTGATCTCT CATGACATGC TGCTGGGCCG CTGGCGGCTT
    TCTCTGGAGC TGTTTGGCCG TGTTTTCATG GAGGACGTTG GAGCAGAACC AGGATCTATC
    CTGACAGAGC TGGGGGGTTT TGAGGTAAAG GAGTCCAAGT TTCGTCGTGA GATGGAGAAG
    CTGCGTAACC TTCAGTCGCG TGATCTGGCC CTGGAGGTGG ACCGAGACCG TGAGCAGCTC
    ATCCAGCAGA CCATGCGGCA GCTGAACGCT CACTTCGGCC GCCGCTGTAC CACCACACCC
    ATGGCCGTGC ACCGCGTAAA GGTGACATTC AAGGATGAGC CTGGAGAGGG CAGTGGTGTC
    GCTCGCAGCT TCTACACCGC CATCGCTCAG GCCTTCTTGT CCAACGACAA GCTGCCCAAT
    CTGGACTGCG TGCAGAGCGT CAGCAAGGGG ATGCAGGCTA GCAATCTTAT GCAGCGGCTG
    AGGAACCGAG ATCGTGAGCG TGAGAGGAGG AGCGGAGGAC TGAGAGCTGC ATCCAGACGA
    GATCGTGACA GCCTAAAGAG ACTTTTTGCT GTGTTTGACT GCAGGGACTC GCGGAGGCAG
    TTGTCCATCG ACACCCGGCC GTTCAGACCT GCATCAGAAG GAAACCCCAG TGATGAACCA
    GAACCGCTGC CGGCTCACAG ACAGGCTCTG GGAGAGAGAC TTTACCCACG AGTTCATGCC
    ATGCAACCGG CGTTTGCTAG TAAAATCACA GGGATGCTGC TGGAACTGTC TCCCGCTCAG
    CTGCTGCTAC TCCTGGCCAG CGAAGACTCG CTCAGAGCCC GAGTGGAGGA GGCCATGGAG
    CTGCTCATAA CTCATGGACG GGAAAATGGT GCTGACAGTA TACTAGACCT CGGCCTCCTC
    GACACTCCTG AGAAAGCACA ACAGGAGAAC CGAAAGAGGC ATGGTTCGAC TCGCAGTGTG
    GTGGACATGG AGCTGGATGA CCCTGATGAT GGGGATGATA ACGCTCCTCT GTTTTACCAG
    CCTGGAAAGA GGGGTTTCTA CTCACCACGT CCCGGCAAGA ACACTGAGGC CAGGCTCAAC
    TGTTTCCGTA ACATCGGCAG GATACTGGGT TTGTGTCTGC TACAGAATGA GCTTTGCCCA
    ATCACTCTCA ACAGACACGT CATCAAGGTG CTGCTCGGCA GAAAGGTGAA CTGGCACGAC
    TTTGCGTTCT TCGACCCGGT AATGTACGAG AGTTTGCGGC AGCTCATCCG TCACTCTCAG
    ACTGAAGAGG CGGAGGCTGT GTTTGCGGCG ATGGACCTGG CCTTCGCTAT CGACCTCTGC
    AAGGAGGAGG GAGCAGGACA GGTGGAGCTG CTGTCCGGTG GAGTCAACAT GCCGGTGACG
    CCTCTGAATG TGTACGAGTA CGTGAGGAGA TACGCAGAGC ACCGGATGCT GGTGGTGGCA
    GAACAGCCTC TGCATGCCAT GCGGAAAGGT CTTCTGGATG TGCTTCCTAA GAACGCTCTG
    GAAGATCTGA CAGCGGAGGA TTTCAGACTG CTGGTCAACG GCTGTGGAGA AGTCAACGTC
    CAGATGCTCA TTAGTTTCAC TTCATTCAAT GATGAGTCAG GTGAGAATGC AGAGAAGCTG
    CTGCAGTTCA AGCGCTGGTT TTGGTCCATC GTTGAGAAGA TGAGCATGAC GGAAAGGCAG
    GACCTGGTGT ACTTCTGGAC GTCCAGCCCG TCTCTGCCGG CCAGTGAAGA AGGTTTCCAG
    CCGATGCCCT CCATCACCAT CCGCCCGCCG GACGACCAGC ACCTGCCCAC AGCCAACACC
    TGCATCTCTC GCCTCTACGT GCCACTTTAC TCTTCCAAAC AGATCCTCAA ACAGAAACTT
    TTACTAGCCA TCAAGACCAA GAACTTCGGT TTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149206
    Clone ID Related Accession (Same CDS sequence) XM_021466628.1
    Accession Version XM_021466628.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGC
    TCCAGTCAGT CTGCCTCTTC ATACATCATC AGGAACCCGC AGCCCCGCCG CAGCAGCCAG
    TCTCAGACAG CCCGCGGACG GGACGAGGAG CAGGATGATA TCGTGTCTGC TGACGTAGAA
    GAGGTGGAGG TGGTGGAGGG TGTTGCAGGT GAGGAGGATC ACCATGATGA CCAGGAGGAG
    CAGGGAGAGG AGAACGCAGA GGCTGAAGGA CAGCATGATG AGCACGATGA GGATGGGAGC
    GACATGGAGC TGGATTTATT GGCTGCCGCT GAGACCGAGA GTGACAGTGA GAGTAACCAT
    AGCAACCAGG ACAATGCGAG TGGCCGCAGG AGTGTTGTGA CGGCAGCCAC TGCTGGATCT
    GAAGCAGGTG CCAGCAGTGT TCCTGCTTTC TTCTCCGAGG ACGACTCCCA GTCGAATGAC
    TCCAGCGACT CGGACAGCAG CAGCAGCCAG AGTGACGATG TGGATCAGGA GACGTTTCTG
    CTGGACGAGC CTCTTGAGAG AACCACCGGC TCAGCACATG CTAACAGTGC AGCCCAGGCC
    CCACGCTCCA TGCAGTGGGC TGTTCGCACT ACACCCAGCC AGCGCTCTGG AGGTGGCGCC
    CCATCCAGCT CGTCCGCTCC TGCTGCGAGC TCCACTGGCC TCATCTACAT CGACCCGTCA
    AACCTGCGCC GCAGCAGCGC CATCAGCACC AGCGCAGCCG CTGCCGCAGC AGCCCTGGAG
    GCCAGTAACT CCAGCAGTTA TCTGACGTCT GCCAGCAGCC TGGCTCGAGC ATACAGCATC
    GTCATCCGCC AGATCTCTGA CCTCATGAGT CTCATTCCCA AATACAACCA CCTGGTCTAC
    TCGCAGTACC CCGCTGCTGT CAAACTCACC TATCAGGATG CTGTCAATCT GCAGAACTTT
    GTGGAGGACA AGTTGATTCC CACCTGGAAC TGGATGGTGT CCATCATGGA CTCCACTGAA
    GCGCAGCTGC GTTACGGCTC GGCTCTGTCT TCAGCCGGGG ATCCTGGTCA TCCCAGCCAC
    CCGCTACACG CATCCCAGCA TGCCGGCCGC AGAGAGCGCA TGACCGCTCG AGAGGAGGCC
    AGTCTGCGCA CTCTAGAAGG ACGAAGGCGT GCTGCTACGC TTCTGACGGC ACGTCAGGGC
    ATGATGTCAG CGCGAGGAGA TTTCCTGAAC TACGCTCTGT CTCTGATGCG TTCCCATAAT
    GACGAGCATT CAGACGTTCT GCCTGTGTTA GATGTCTGTT CGCTCAAACA CGTGGCTTAC
    GTCTTCCAGG CGCTCATCTA CTGGATTAAA GCCATGAATC TGCAGACCAC GCTCGACACC
    ACTCAGATTG ACAGGAAGAG GAATCGTGAG CTGCTGGAGC TCGGTCTGGA CAATGAAGAC
    TCTGAGCACG AGAACGATGA GGACACCAAT CAGAGCTCTA CACTGCAGGA TAAGGACGAG
    GAGCCTGTGC CTGCAGAGAC TGGACAACAC CATCCGTTTT TCCGCCGCTC GGACTCCATG
    ACCTTCCTGG GGTGCATCCC TCCAAACCCT TTTGAAGTTC CTCTAGCAGA GGCCATTCCT
    CTGGCTGACC AGCCGCATCT CCTGCAGCCC AATGCCAGAA AGGAGGATTT GTTTGGACGG
    CCTAGTCAAG GTCTATATTC GTCCTCATAT ATGGCCAGTA AAGGCCTCAC CGATCTGACT
    GTGGACATGA ATTGCTTGCA GATGTCGTAT TCGGCGAACA TGAAGAACGT GATGAGTATG
    GAGTCCCGGC AGAGGGGTGG CGAGGAGCAG CCGGTGGCGG AGCAGGAGAT GGATGTGTCT
    AAACCCGGCC CTTCACCACA CGATCTGGCG GCCCAGTTAA AGAGCAGCCT GCTGGCAGAG
    ATTGGCCTGA CTGAGAGTGA TGGCCCACCG CTGCCCACGT TTATTCCACA CTGTAGTTTT
    ATGGGGATGG TGATCTCTCA TGACATGCTG CTGGGCCGCT GGCGGCTTTC TCTGGAGCTG
    TTTGGCCGTG TTTTCATGGA GGACGTTGGA GCAGAACCAG GATCTATCCT GACAGAGCTG
    GGGGGTTTTG AGGTAAAGGA GTCCAAGTTT CGTCGTGAGA TGGAGAAGCT GCGTAACCTT
    CAGTCGCGTG ATCTGGCCCT GGAGGTGGAC CGAGACCGTG AGCAGCTCAT CCAGCAGACC
    ATGCGGCAGC TGAACGCTCA CTTCGGCCGC CGCTGTACCA CCACACCCAT GGCCGTGCAC
    CGCGTAAAGG TGACATTCAA GGATGAGCCT GGAGAGGGCA GTGGTGTCGC TCGCAGCTTC
    TACACCGCCA TCGCTCAGGC CTTCTTGTCC AACGACAAGC TGCCCAATCT GGACTGCGTG
    CAGAGCGTCA GCAAGGGGAT GCAGGCTAGC AATCTTATGC AGCGGCTGAG GAACCGAGAT
    CGTGAGCGTG AGAGGAGGAG CGGAGGACTG AGAGCTGCAT CCAGACGAGA TCGTGACAGC
    CTAAAGAGAC TTTTTGCTGT GTTTGACTGC AGGGACTCGC GGAGGCAGTT GTCCATCGAC
    ACCCGGCCGT TCAGACCTGC ATCAGAAGGA AACCCCAGTG ATGAACCAGA ACCGCTGCCG
    GCTCACAGAC AGGCTCTGGG AGAGAGACTT TACCCACGAG TTCATGCCAT GCAACCGGCG
    TTTGCTAGTA AAATCACAGG GATGCTGCTG GAACTGTCTC CCGCTCAGCT GCTGCTACTC
    CTGGCCAGCG AAGACTCGCT CAGAGCCCGA GTGGAGGAGG CCATGGAGCT GCTCATAACT
    CATGGACGGG AAAATGGTGC TGACAGTATA CTAGACCTCG GCCTCCTCGA CACTCCTGAG
    AAAGCACAAC AGGAGAACCG AAAGAGGCAT GGTTCGACTC GCAGTGTGGT GGACATGGAG
    CTGGATGACC CTGATGATGG GGATGATAAC GCTCCTCTGT TTTACCAGCC TGGAAAGAGG
    GGTTTCTACT CACCACGTCC CGGCAAGAAC ACTGAGGCCA GGCTCAACTG TTTCCGTAAC
    ATCGGCAGGA TACTGGGTTT GTGTCTGCTA CAGAATGAGC TTTGCCCAAT CACTCTCAAC
    AGACACGTCA TCAAGGTGCT GCTCGGCAGA AAGGTGAACT GGCACGACTT TGCGTTCTTC
    GACCCGGTAA TGTACGAGAG TTTGCGGCAG CTCATCCGTC ACTCTCAGAC TGAAGAGGCG
    GAGGCTGTGT TTGCGGCGAT GGACCTGGCC TTCGCTATCG ACCTCTGCAA GGAGGAGGGA
    GCAGGACAGG TGGAGCTGCT GTCCGGTGGA GTCAACATGC CGGTGACGCC TCTGAATGTG
    TACGAGTACG TGAGGAGATA CGCAGAGCAC CGGATGCTGG TGGTGGCAGA ACAGCCTCTG
    CATGCCATGC GGAAAGGTCT TCTGGATGTG CTTCCTAAGA ACGCTCTGGA AGATCTGACA
    GCGGAGGATT TCAGACTGCT GGTCAACGGC TGTGGAGAAG TCAACGTCCA GATGCTCATT
    AGTTTCACTT CATTCAATGA TGAGTCAGGT GAGAATGCAG AGAAGCTGCT GCAGTTCAAG
    CGCTGGTTTT GGTCCATCGT TGAGAAGATG AGCATGACGG AAAGGCAGGA CCTGGTGTAC
    TTCTGGACGT CCAGCCCGTC TCTGCCGGCC AGTGAAGAAG GTTTCCAGCC GATGCCCTCC
    ATCACCATCC GCCCGCCGGA CGACCAGCAC CTGCCCACAG CCAACACCTG CATCTCTCGC
    CTCTACGTGC CACTTTACTC TTCCAAACAG ATCCTCAAAC AGAAACTTTT ACTAGCCATC
    AAGACCAAGA ACTTCGGTTT TGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322303.1
    CDS173..8659
    Translation

    Target ORF information:

    RefSeq Version XM_021466628.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466628.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGC
    TCCAGTCAGT CTGCCTCTTC ATACATCATC AGGAACCCGC AGCCCCGCCG CAGCAGCCAG
    TCTCAGACAG CCCGCGGACG GGACGAGGAG CAGGATGATA TCGTGTCTGC TGACGTAGAA
    GAGGTGGAGG TGGTGGAGGG TGTTGCAGGT GAGGAGGATC ACCATGATGA CCAGGAGGAG
    CAGGGAGAGG AGAACGCAGA GGCTGAAGGA CAGCATGATG AGCACGATGA GGATGGGAGC
    GACATGGAGC TGGATTTATT GGCTGCCGCT GAGACCGAGA GTGACAGTGA GAGTAACCAT
    AGCAACCAGG ACAATGCGAG TGGCCGCAGG AGTGTTGTGA CGGCAGCCAC TGCTGGATCT
    GAAGCAGGTG CCAGCAGTGT TCCTGCTTTC TTCTCCGAGG ACGACTCCCA GTCGAATGAC
    TCCAGCGACT CGGACAGCAG CAGCAGCCAG AGTGACGATG TGGATCAGGA GACGTTTCTG
    CTGGACGAGC CTCTTGAGAG AACCACCGGC TCAGCACATG CTAACAGTGC AGCCCAGGCC
    CCACGCTCCA TGCAGTGGGC TGTTCGCACT ACACCCAGCC AGCGCTCTGG AGGTGGCGCC
    CCATCCAGCT CGTCCGCTCC TGCTGCGAGC TCCACTGGCC TCATCTACAT CGACCCGTCA
    AACCTGCGCC GCAGCAGCGC CATCAGCACC AGCGCAGCCG CTGCCGCAGC AGCCCTGGAG
    GCCAGTAACT CCAGCAGTTA TCTGACGTCT GCCAGCAGCC TGGCTCGAGC ATACAGCATC
    GTCATCCGCC AGATCTCTGA CCTCATGAGT CTCATTCCCA AATACAACCA CCTGGTCTAC
    TCGCAGTACC CCGCTGCTGT CAAACTCACC TATCAGGATG CTGTCAATCT GCAGAACTTT
    GTGGAGGACA AGTTGATTCC CACCTGGAAC TGGATGGTGT CCATCATGGA CTCCACTGAA
    GCGCAGCTGC GTTACGGCTC GGCTCTGTCT TCAGCCGGGG ATCCTGGTCA TCCCAGCCAC
    CCGCTACACG CATCCCAGCA TGCCGGCCGC AGAGAGCGCA TGACCGCTCG AGAGGAGGCC
    AGTCTGCGCA CTCTAGAAGG ACGAAGGCGT GCTGCTACGC TTCTGACGGC ACGTCAGGGC
    ATGATGTCAG CGCGAGGAGA TTTCCTGAAC TACGCTCTGT CTCTGATGCG TTCCCATAAT
    GACGAGCATT CAGACGTTCT GCCTGTGTTA GATGTCTGTT CGCTCAAACA CGTGGCTTAC
    GTCTTCCAGG CGCTCATCTA CTGGATTAAA GCCATGAATC TGCAGACCAC GCTCGACACC
    ACTCAGATTG ACAGGAAGAG GAATCGTGAG CTGCTGGAGC TCGGTCTGGA CAATGAAGAC
    TCTGAGCACG AGAACGATGA GGACACCAAT CAGAGCTCTA CACTGCAGGA TAAGGACGAG
    GAGCCTGTGC CTGCAGAGAC TGGACAACAC CATCCGTTTT TCCGCCGCTC GGACTCCATG
    ACCTTCCTGG GGTGCATCCC TCCAAACCCT TTTGAAGTTC CTCTAGCAGA GGCCATTCCT
    CTGGCTGACC AGCCGCATCT CCTGCAGCCC AATGCCAGAA AGGAGGATTT GTTTGGACGG
    CCTAGTCAAG GTCTATATTC GTCCTCATAT ATGGCCAGTA AAGGCCTCAC CGATCTGACT
    GTGGACATGA ATTGCTTGCA GATGTCGTAT TCGGCGAACA TGAAGAACGT GATGAGTATG
    GAGTCCCGGC AGAGGGGTGG CGAGGAGCAG CCGGTGGCGG AGCAGGAGAT GGATGTGTCT
    AAACCCGGCC CTTCACCACA CGATCTGGCG GCCCAGTTAA AGAGCAGCCT GCTGGCAGAG
    ATTGGCCTGA CTGAGAGTGA TGGCCCACCG CTGCCCACGT TTATTCCACA CTGTAGTTTT
    ATGGGGATGG TGATCTCTCA TGACATGCTG CTGGGCCGCT GGCGGCTTTC TCTGGAGCTG
    TTTGGCCGTG TTTTCATGGA GGACGTTGGA GCAGAACCAG GATCTATCCT GACAGAGCTG
    GGGGGTTTTG AGGTAAAGGA GTCCAAGTTT CGTCGTGAGA TGGAGAAGCT GCGTAACCTT
    CAGTCGCGTG ATCTGGCCCT GGAGGTGGAC CGAGACCGTG AGCAGCTCAT CCAGCAGACC
    ATGCGGCAGC TGAACGCTCA CTTCGGCCGC CGCTGTACCA CCACACCCAT GGCCGTGCAC
    CGCGTAAAGG TGACATTCAA GGATGAGCCT GGAGAGGGCA GTGGTGTCGC TCGCAGCTTC
    TACACCGCCA TCGCTCAGGC CTTCTTGTCC AACGACAAGC TGCCCAATCT GGACTGCGTG
    CAGAGCGTCA GCAAGGGGAT GCAGGCTAGC AATCTTATGC AGCGGCTGAG GAACCGAGAT
    CGTGAGCGTG AGAGGAGGAG CGGAGGACTG AGAGCTGCAT CCAGACGAGA TCGTGACAGC
    CTAAAGAGAC TTTTTGCTGT GTTTGACTGC AGGGACTCGC GGAGGCAGTT GTCCATCGAC
    ACCCGGCCGT TCAGACCTGC ATCAGAAGGA AACCCCAGTG ATGAACCAGA ACCGCTGCCG
    GCTCACAGAC AGGCTCTGGG AGAGAGACTT TACCCACGAG TTCATGCCAT GCAACCGGCG
    TTTGCTAGTA AAATCACAGG GATGCTGCTG GAACTGTCTC CCGCTCAGCT GCTGCTACTC
    CTGGCCAGCG AAGACTCGCT CAGAGCCCGA GTGGAGGAGG CCATGGAGCT GCTCATAACT
    CATGGACGGG AAAATGGTGC TGACAGTATA CTAGACCTCG GCCTCCTCGA CACTCCTGAG
    AAAGCACAAC AGGAGAACCG AAAGAGGCAT GGTTCGACTC GCAGTGTGGT GGACATGGAG
    CTGGATGACC CTGATGATGG GGATGATAAC GCTCCTCTGT TTTACCAGCC TGGAAAGAGG
    GGTTTCTACT CACCACGTCC CGGCAAGAAC ACTGAGGCCA GGCTCAACTG TTTCCGTAAC
    ATCGGCAGGA TACTGGGTTT GTGTCTGCTA CAGAATGAGC TTTGCCCAAT CACTCTCAAC
    AGACACGTCA TCAAGGTGCT GCTCGGCAGA AAGGTGAACT GGCACGACTT TGCGTTCTTC
    GACCCGGTAA TGTACGAGAG TTTGCGGCAG CTCATCCGTC ACTCTCAGAC TGAAGAGGCG
    GAGGCTGTGT TTGCGGCGAT GGACCTGGCC TTCGCTATCG ACCTCTGCAA GGAGGAGGGA
    GCAGGACAGG TGGAGCTGCT GTCCGGTGGA GTCAACATGC CGGTGACGCC TCTGAATGTG
    TACGAGTACG TGAGGAGATA CGCAGAGCAC CGGATGCTGG TGGTGGCAGA ACAGCCTCTG
    CATGCCATGC GGAAAGGTCT TCTGGATGTG CTTCCTAAGA ACGCTCTGGA AGATCTGACA
    GCGGAGGATT TCAGACTGCT GGTCAACGGC TGTGGAGAAG TCAACGTCCA GATGCTCATT
    AGTTTCACTT CATTCAATGA TGAGTCAGGT GAGAATGCAG AGAAGCTGCT GCAGTTCAAG
    CGCTGGTTTT GGTCCATCGT TGAGAAGATG AGCATGACGG AAAGGCAGGA CCTGGTGTAC
    TTCTGGACGT CCAGCCCGTC TCTGCCGGCC AGTGAAGAAG GTTTCCAGCC GATGCCCTCC
    ATCACCATCC GCCCGCCGGA CGACCAGCAC CTGCCCACAG CCAACACCTG CATCTCTCGC
    CTCTACGTGC CACTTTACTC TTCCAAACAG ATCCTCAAAC AGAAACTTTT ACTAGCCATC
    AAGACCAAGA ACTTCGGTTT TGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149207
    Clone ID Related Accession (Same CDS sequence) XM_021466629.1
    Accession Version XM_021466629.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGAGA GAGACTCTGA GCTGCTCCGC GAGCGTGAGT CGGTCCTGAG GCTGCGGGAG
    AGGCGGTGGC TGGACGGGGC CTCCTTCGAT GCGGAGCGTG GCTCCACCAG CCGCGAGGGA
    GAGCCCAGCC TTGACAAGAA GAGTGTCCCG CTGCAGAGCC CAGTCACTCT GGGGGAAGAG
    CTCCAGTGGT GGCCTGATAA GGACTATGTA ACAAAGTTTG TCAGCATTGG TGCTTTGTAC
    TCGGAGCTGG TTGCCGTCAG TACCAAAGGA GAACTCTACC AGTGGAAGTG GAATGAACCT
    GAACCTTACA GAAACGCACA AAATCCTTCT ATACATCACC CTCGTGTTCC TTTCCTGGGC
    TTGACCAATG AAAAGATCAC CCACCTGTCC GCCAACAGTA TCCGAGCCAC TGTGGCCACC
    GAGAACAACA AGGTGGCAAC ATGGGTGGAT GAAACTCTGA GCACTGTTGC TGCTAAACTC
    GAACACGGAG CACAAACGTT CCCGGAGCTG CAGGGGGAGC GGATCGTCTC TCTGCACTGC
    TGTGCCCTGT ATACCTGCGC TCAGCTGGAG AACAGCCTTT ACTGGTGGGG TGTTGTGCCT
    TTTAGTCAAC GGAAAAAAAT GCTTGAAAAG GCCAGAGCCA AGAACAAGAA ACCAAAGTCC
    AGTGCTGGAA TCTCTTCGAT ACCCAACATC ACAGTAGGAA CTCAGGTGTG CCTGAGGAAT
    AATCCTCTTT ATCACGCTGG TGCCGTGGCC TTTTCCGTGA ACGCCGGGAT CCCTAAAGTG
    GGCGTCCTGC TGGAGTCGGT GTGGAACATG AACGACAGCT GCCGCTTCCA GCTGCGCTCG
    CCAGAGAGCC TGAAAAACAT GGAGAAAAAC ACCAAGACAC AGGAGACCAA GACGGAGAGC
    AAGCCAGAGC TGGTGAAGAC GGAAATGGGC CCTCCGCCCT CACCAGCGTC CACATGCAGT
    GACACTTCAT CCATCGCTAG CAGCGCTTCA CTGCCGTACA AGCGAAGGCG CTCAACACCG
    GCTCCGAAGG AGGAGGAGAA GGTGAATGAG GAGCAGTGGC CTCTGCGAGA GGTTGTTTTC
    GTGGAGGATG TTAAAAACGT CCCCGTGGGA AAGGTGCTAA AAGTCGATGG TGCATATGTT
    GCTGTGAAAT TTCCAGGGAC GTCGAGCAGC GTGAGCAGCC AGAGCGCAGC TCCCACTGAC
    TCTGACCCGT CCTCACTGCT GCAAGACTGT CGACTGCTCA GAATAGATGA GCTACAGGTT
    GTTAAAACTG GTGGAACACC AAAAGTTCCT GACTGTTTTC AGCGAACACC TAAAAAACTT
    TGCATCCCAG AAAAAGCTGA AATTTTAGCA GTGAATGTTG ACTCCAAAGG AGTTCATGCT
    GTGTTGAAAA CGGGCAGCTG GGTGAGATAC TGTGTCTTTG ACTTAGCCAC CGGCAAAGCC
    GAGCAAGAGA ATCACTTTCC CACCAGTAAC CTGGCGTTTC TTGGTCAGAG CGAGCGAAAT
    GTGGCCATCT TCACTGCTGG CCAGGAGAGT CCCATTATTT TGAGGGATGG CAATGGCACT
    ATTTATCCCA TGGCTAAAGA CTGTATGGGT GGAATACGTG ACCCTGATTG GCTGGATCTG
    CCGCCTATAG CCAGTCTGGG CATGGGAGTT CACTCGCTCG CCAACCTTCC CAGTAACTCC
    ACAATCAAAA AGAAGGCGGC CATCATCATA ATGGCTGTAG AGAAGCAGAC TCTGATGCAG
    CACGTGCTGC GCTGTGATTA CGAGGCGTGT AGGCAGTATC TGATCAGTCT GGAGCAGGCC
    ATGCTACTGG AGCAGAACCC TCACGCTCTG GACACTCTGC TAGGACACCG CTGTGACGGA
    AACCGCAACG TCCTCCATGC CTGTGTGTCC GTCTGCTTCC CCGTCAGCAA CAAGGAAACC
    AAGGAGGAAG AAGAAGCAGA GCGGTCTGAA AGAAACACGT TCGCTGAGAG GCTTTCTGCA
    GTGGAGGCCA TTGCCAATGC CATATCAGTG GTGTCGAGCA ACAGCTCCGG GAACCGGACA
    GGATCATCCA GCAGCAGGGG CTTGCGTTTG AGAGAGATGA TGCGGCGCTC ACTCCGTGCT
    GCAGGTTTAG GCCGACATGA ATCCGGACCT TCCTCCAGCG ACCATCAAGA CCCCGTTTCA
    CCCCCGATAG CACCTCCTAG CTGGGTACCA GACCCTCCAC CGATGGATCC TGACGGTGAC
    ATTGATTTCA TCTTGGCCCC TGCAGTGGGC TCCCTCACCA CTGCTTCCAC AGGCACCAGC
    CAGGGACCCA GCACTTCCAC CATCCCAGGA CCCTCCTCTG AACCATCAGT AGTGGAATCT
    AAAGACCGTA AGGCGAACGC ACACCTGATA CTGAAGCTGA TGTGTGACAG CATCGTCCTG
    CGGCCTCATC TAAGAGAACT GCTCTCTGCC AAGGATGCTC GTGGAATGAC TCCATTCATG
    TTAGCAGTGA GTGGCAGAGC TTACCCAGCA GCCATTACTG TCTTAGAAGC AGCACAGAAA
    ATTGCCAAAG GTGAACCAGG TCTTGGAGAG AAAGAGGATA CAGCATCTGT GTTCATGGAG
    ATGATCTGTC CTTCAGGAAC CAATCCAGAC GACTCTCCGC TGTACGTCCT GTGCTGTAAC
    GACACCTGCA GCTTCACCTG GACTGGAGCA GAACACATCA ACCAGGACAT CTTTGAGTGC
    AGAACTTGTG GCCTGCTGGA GTCGCTGTGC TGCTGCACTG AATGTGCTAG AGTCTGTCAC
    AAGGGGCACG ACTGCAAACT GAAGAGGACG TCACCAACAG CTTACTGTGA CTGCTGGGAG
    AAGTGCAAAT GCAAGACTCT CATCGCAGGC CAGAAAGCGG CACGTCTGGA TCTGCTCTAC
    AGGCTGCTGA CCACCACTAA TCTAGTGACC ACTCCTAACA GCAGGGGAGA GCACATCTTG
    CTGTTTCTGG TGCAGACTGT TGCAAGGCAG AGTGTCGAAC ACTGTCAGTA TCGACCTCCT
    CGCATTCGAG AGGACAGGAA CCGGAAAGCT GCCAGTGCTG AAGACTCTGA TATGCCAGAT
    CATGACCTGG AACCTCCACG CTTTGCTCAG CTGGCTCTGG AGCGAGTGTT GCAGGACTGG
    AACGCACTGA AGTCAATGAT AATGTTCGGC TCTCAGGAAA ATAAAGATCC GTTAAGCGCT
    AGCAGCAGAA TCGCTCACCT TTTACCGGAG GAGCAGATGT ACCTCAACCA GCAGAGTGGC
    ACCATCAGAC TGGACTGTTT CACACACTGC CTTATTGTCA AGTGTGCACC AGACATCACT
    TTCATTGACA CACTGTTAGG GACCCTGGTG AAGGAGCTGC AGAATAAATA CACCCCCGGC
    CGGCGGGAAG AGGCCATCAA TGTCACACGG AGGTTCTTGC GCTCTGTGGC CAGAGTGTTT
    GTCATCCTCA GCGTGGAGAT GGCATCTTCC AAGAAAAAGA ACAACTTCAT CCCTCAGCCT
    ATTGGGAAGT GCAGGCGTGT GTTCCAGGCA CTGTTGCCGT ACGCAGTGGA GGAGCTCTGC
    AATGTGGCCG AGTCTCTGAT CGTGCCGGTG CGTATGGGCA TCGCTCGGCC CACAGCTCCC
    TTCACTCTGG CCAGTACCAG CATTGATGCA GTGCAGGGCA GTGAGGAGCT CTTCTCTGTG
    GAGCCTCTGC CACCTCGACC CTCCCCGGAC CAGTCCAGCA GCTCCAGTCA GTCTGCCTCT
    TCATACATCA TCAGGAACCC GCAGCCCCGC CGCAGCAGCC AGTCTCAGAC AGCCCGCGGA
    CGGGACGAGG AGCAGGATGA TATCGTGTCT GCTGACGTAG AAGAGGTGGA GGTGGTGGAG
    GGTGTTGCAG GTGAGGAGGA TCACCATGAT GACCAGGAGG AGCAGGGAGA GGAGAACGCA
    GAGGCTGAAG GACAGCATGA TGAGCACGAT GAGGATGGGA GCGACATGGA GCTGGATTTA
    TTGGCTGCCG CTGAGACCGA GAGTGACAGT GAGAGTAACC ATAGCAACCA GGACAATGCG
    AGTGGCCGCA GGAGTGTTGT GACGGCAGCC ACTGCTGGAT CTGAAGCAGG TGCCAGCAGT
    GTTCCTGCTT TCTTCTCCGA GGACGACTCC CAGTCGAATG ACTCCAGCGA CTCGGACAGC
    AGCAGCAGCC AGAGTGACGA TGTGGATCAG GAGACGTTTC TGCTGGACGA GCCTCTTGAG
    AGAACCACCG GCTCAGCACA TGCTAACAGT GCAGCCCAGG CCCCACGCTC CATGCAGTGG
    GCTGTTCGCA CTACACCCAG CCAGCGCTCT GGAGGTGGCG CCCCATCCAG CTCGTCCGCT
    CCTGCTGCGA GCTCCACTGG CCTCATCTAC ATCGACCCGT CAAACCTGCG CCGCAGCAGC
    GCCATCAGCA CCAGCGCAGC CGCTGCCGCA GCAGCCCTGG AGGCCAGTAA CTCCAGCAGT
    TATCTGACGT CTGCCAGCAG CCTGGCTCGA GCATACAGCA TCGTCATCCG CCAGATCTCT
    GACCTCATGA GTCTCATTCC CAAATACAAC CACCTGGTCT ACTCGCAGTA CCCCGCTGCT
    GTCAAACTCA CCTATCAGGA TGCTGTCAAT CTGCAGAACT TTGTGGAGGA CAAGTTGATT
    CCCACCTGGA ACTGGATGGT GTCCATCATG GACTCCACTG AAGCGCAGCT GCGTTACGGC
    TCGGCTCTGT CTTCAGCCGG GGATCCTGGT CATCCCAGCC ACCCGCTACA CGCATCCCAG
    CATGCCGGCC GCAGAGAGCG CATGACCGCT CGAGAGGAGG CCAGTCTGCG CACTCTAGAA
    GGACGAAGGC GTGCTGCTAC GCTTCTGACG GCACGTCAGG GCATGATGTC AGCGCGAGGA
    GATTTCCTGA ACTACGCTCT GTCTCTGATG CGTTCCCATA ATGACGAGCA TTCAGACGTT
    CTGCCTGTGT TAGATGTCTG TTCGCTCAAA CACGTGGCTT ACGTCTTCCA GGCGCTCATC
    TACTGGATTA AAGCCATGAA TCTGCAGACC ACGCTCGACA CCACTCAGAT TGACAGGAAG
    AGGAATCGTG AGCTGCTGGA GCTCGGTCTG GACAATGAAG ACTCTGAGCA CGAGAACGAT
    GAGGACACCA ATCAGAGCTC TACACTGCAG GATAAGGACG AGGAGCCTGT GCCTGCAGAG
    ACTGGACAAC ACCATCCGTT TTTCCGCCGC TCGGACTCCA TGACCTTCCT GGGGTGCATC
    CCTCCAAACC CTTTTGAAGT TCCTCTAGCA GAGGCCATTC CTCTGGCTGA CCAGCCGCAT
    CTCCTGCAGC CCAATGCCAG AAAGGAGGAT TTGTTTGGAC GGCCTAGTCA AGGTCTATAT
    TCGTCCTCAT ATATGGCCAG TAAAGGCCTC ACCGATCTGA CTGTGGACAT GAATTGCTTG
    CAGATTCTAC CCACAAAGAT GTCGTATTCG GCGAACATGA AGAACGTGAT GAGTATGGAG
    TCCCGGCAGA GGGGTGGCGA GGAGCAGCCG GTGGCGGAGC AGGAGATGGA TGTGTCTAAA
    CCCGGCCCTT CACCACACGA TCTGGCGGCC CAGTTAAAGA GCAGCCTGCT GGCAGAGATT
    GGCCTGACTG AGAGTGATGG CCCACCGCTG CCCACGTTTA TTCCACACTG TAGTTTTATG
    GGGATGGTGA TCTCTCATGA CATGCTGCTG GGCCGCTGGC GGCTTTCTCT GGAGCTGTTT
    GGCCGTGTTT TCATGGAGGA CGTTGGAGCA GAACCAGGAT CTATCCTGAC AGAGCTGGGG
    GGTTTTGAGG TAAAGGAGTC CAAGTTTCGT CGTGAGATGG AGAAGCTGCG TAACCTTCAG
    TCGCGTGATC TGGCCCTGGA GGTGGACCGA GACCGTGAGC AGCTCATCCA GCAGACCATG
    CGGCAGCTGA ACGCTCACTT CGGCCGCCGC TGTACCACCA CACCCATGGC CGTGCACCGC
    GTAAAGGTGA CATTCAAGGA TGAGCCTGGA GAGGGCAGTG GTGTCGCTCG CAGCTTCTAC
    ACCGCCATCG CTCAGGCCTT CTTGTCCAAC GACAAGCTGC CCAATCTGGA CTGCGTGCAG
    AGCGTCAGCA AGGGGATGCA GGCTAGCAAT CTTATGCAGC GGCTGAGGAA CCGAGATCGT
    GAGCGTGAGA GGAGGAGCGG AGGACTGAGA GCTGCATCCA GACGAGATCG TGACAGCCTA
    AAGAGACTTT TTGCTGTGTT TGACTGCAGG GACTCGCGGA GGCAGTTGTC CATCGACACC
    CGGCCGTTCA GACCTGCATC AGAAGGAAAC CCCAGTGATG AACCAGAACC GCTGCCGGCT
    CACAGACAGG CTCTGGGAGA GAGACTTTAC CCACGAGTTC ATGCCATGCA ACCGGCGTTT
    GCTAGTAAAA TCACAGGGAT GCTGCTGGAA CTGTCTCCCG CTCAGCTGCT GCTACTCCTG
    GCCAGCGAAG ACTCGCTCAG AGCCCGAGTG GAGGAGGCCA TGGAGCTGCT CATAACTCAT
    GGACGGGAAA ATGGTGCTGA CAGTATACTA GACCTCGGCC TCCTCGACAC TCCTGAGAAA
    GCACAACAGG AGAACCGAAA GAGGCATGGT TCGACTCGCA GTGTGGTGGA CATGGAGCTG
    GATGACCCTG ATGATGGGGA TGATAACGCT CCTCTGTTTT ACCAGCCTGG AAAGAGGGGT
    TTCTACTCAC CACGTCCCGG CAAGAACACT GAGGCCAGGC TCAACTGTTT CCGTAACATC
    GGCAGGATAC TGGGTTTGTG TCTGCTACAG AATGAGCTTT GCCCAATCAC TCTCAACAGA
    CACGTCATCA AGGTGCTGCT CGGCAGAAAG GTGAACTGGC ACGACTTTGC GTTCTTCGAC
    CCGGTAATGT ACGAGAGTTT GCGGCAGCTC ATCCGTCACT CTCAGACTGA AGAGGCGGAG
    GCTGTGTTTG CGGCGATGGA CCTGGCCTTC GCTATCGACC TCTGCAAGGA GGAGGGAGCA
    GGACAGGTGG AGCTGCTGTC CGGTGGAGTC AACATGCCGG TGACGCCTCT GAATGTGTAC
    GAGTACGTGA GGAGATACGC AGAGCACCGG ATGCTGGTGG TGGCAGAACA GCCTCTGCAT
    GCCATGCGGA AAGGTCTTCT GGATGTGCTT CCTAAGAACG CTCTGGAAGA TCTGACAGCG
    GAGGATTTCA GACTGCTGGT CAACGGCTGT GGAGAAGTCA ACGTCCAGAT GCTCATTAGT
    TTCACTTCAT TCAATGATGA GTCAGGTGAG AATGCAGAGA AGCTGCTGCA GTTCAAGCGC
    TGGTTTTGGT CCATCGTTGA GAAGATGAGC ATGACGGAAA GGCAGGACCT GGTGTACTTC
    TGGACGTCCA GCCCGTCTCT GCCGGCCAGT GAAGAAGGTT TCCAGCCGAT GCCCTCCATC
    ACCATCCGCC CGCCGGACGA CCAGCACCTG CCCACAGCCA ACACCTGCAT CTCTCGCCTC
    TACGTGCCAC TTTACTCTTC CAAACAGATC CTCAAACAGA AACTTTTACT AGCCATCAAG
    ACCAAGAACT TCGGTTTTGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322304.1
    CDS173..8656
    Translation

    Target ORF information:

    RefSeq Version XM_021466629.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466629.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGAGA GAGACTCTGA GCTGCTCCGC GAGCGTGAGT CGGTCCTGAG GCTGCGGGAG
    AGGCGGTGGC TGGACGGGGC CTCCTTCGAT GCGGAGCGTG GCTCCACCAG CCGCGAGGGA
    GAGCCCAGCC TTGACAAGAA GAGTGTCCCG CTGCAGAGCC CAGTCACTCT GGGGGAAGAG
    CTCCAGTGGT GGCCTGATAA GGACTATGTA ACAAAGTTTG TCAGCATTGG TGCTTTGTAC
    TCGGAGCTGG TTGCCGTCAG TACCAAAGGA GAACTCTACC AGTGGAAGTG GAATGAACCT
    GAACCTTACA GAAACGCACA AAATCCTTCT ATACATCACC CTCGTGTTCC TTTCCTGGGC
    TTGACCAATG AAAAGATCAC CCACCTGTCC GCCAACAGTA TCCGAGCCAC TGTGGCCACC
    GAGAACAACA AGGTGGCAAC ATGGGTGGAT GAAACTCTGA GCACTGTTGC TGCTAAACTC
    GAACACGGAG CACAAACGTT CCCGGAGCTG CAGGGGGAGC GGATCGTCTC TCTGCACTGC
    TGTGCCCTGT ATACCTGCGC TCAGCTGGAG AACAGCCTTT ACTGGTGGGG TGTTGTGCCT
    TTTAGTCAAC GGAAAAAAAT GCTTGAAAAG GCCAGAGCCA AGAACAAGAA ACCAAAGTCC
    AGTGCTGGAA TCTCTTCGAT ACCCAACATC ACAGTAGGAA CTCAGGTGTG CCTGAGGAAT
    AATCCTCTTT ATCACGCTGG TGCCGTGGCC TTTTCCGTGA ACGCCGGGAT CCCTAAAGTG
    GGCGTCCTGC TGGAGTCGGT GTGGAACATG AACGACAGCT GCCGCTTCCA GCTGCGCTCG
    CCAGAGAGCC TGAAAAACAT GGAGAAAAAC ACCAAGACAC AGGAGACCAA GACGGAGAGC
    AAGCCAGAGC TGGTGAAGAC GGAAATGGGC CCTCCGCCCT CACCAGCGTC CACATGCAGT
    GACACTTCAT CCATCGCTAG CAGCGCTTCA CTGCCGTACA AGCGAAGGCG CTCAACACCG
    GCTCCGAAGG AGGAGGAGAA GGTGAATGAG GAGCAGTGGC CTCTGCGAGA GGTTGTTTTC
    GTGGAGGATG TTAAAAACGT CCCCGTGGGA AAGGTGCTAA AAGTCGATGG TGCATATGTT
    GCTGTGAAAT TTCCAGGGAC GTCGAGCAGC GTGAGCAGCC AGAGCGCAGC TCCCACTGAC
    TCTGACCCGT CCTCACTGCT GCAAGACTGT CGACTGCTCA GAATAGATGA GCTACAGGTT
    GTTAAAACTG GTGGAACACC AAAAGTTCCT GACTGTTTTC AGCGAACACC TAAAAAACTT
    TGCATCCCAG AAAAAGCTGA AATTTTAGCA GTGAATGTTG ACTCCAAAGG AGTTCATGCT
    GTGTTGAAAA CGGGCAGCTG GGTGAGATAC TGTGTCTTTG ACTTAGCCAC CGGCAAAGCC
    GAGCAAGAGA ATCACTTTCC CACCAGTAAC CTGGCGTTTC TTGGTCAGAG CGAGCGAAAT
    GTGGCCATCT TCACTGCTGG CCAGGAGAGT CCCATTATTT TGAGGGATGG CAATGGCACT
    ATTTATCCCA TGGCTAAAGA CTGTATGGGT GGAATACGTG ACCCTGATTG GCTGGATCTG
    CCGCCTATAG CCAGTCTGGG CATGGGAGTT CACTCGCTCG CCAACCTTCC CAGTAACTCC
    ACAATCAAAA AGAAGGCGGC CATCATCATA ATGGCTGTAG AGAAGCAGAC TCTGATGCAG
    CACGTGCTGC GCTGTGATTA CGAGGCGTGT AGGCAGTATC TGATCAGTCT GGAGCAGGCC
    ATGCTACTGG AGCAGAACCC TCACGCTCTG GACACTCTGC TAGGACACCG CTGTGACGGA
    AACCGCAACG TCCTCCATGC CTGTGTGTCC GTCTGCTTCC CCGTCAGCAA CAAGGAAACC
    AAGGAGGAAG AAGAAGCAGA GCGGTCTGAA AGAAACACGT TCGCTGAGAG GCTTTCTGCA
    GTGGAGGCCA TTGCCAATGC CATATCAGTG GTGTCGAGCA ACAGCTCCGG GAACCGGACA
    GGATCATCCA GCAGCAGGGG CTTGCGTTTG AGAGAGATGA TGCGGCGCTC ACTCCGTGCT
    GCAGGTTTAG GCCGACATGA ATCCGGACCT TCCTCCAGCG ACCATCAAGA CCCCGTTTCA
    CCCCCGATAG CACCTCCTAG CTGGGTACCA GACCCTCCAC CGATGGATCC TGACGGTGAC
    ATTGATTTCA TCTTGGCCCC TGCAGTGGGC TCCCTCACCA CTGCTTCCAC AGGCACCAGC
    CAGGGACCCA GCACTTCCAC CATCCCAGGA CCCTCCTCTG AACCATCAGT AGTGGAATCT
    AAAGACCGTA AGGCGAACGC ACACCTGATA CTGAAGCTGA TGTGTGACAG CATCGTCCTG
    CGGCCTCATC TAAGAGAACT GCTCTCTGCC AAGGATGCTC GTGGAATGAC TCCATTCATG
    TTAGCAGTGA GTGGCAGAGC TTACCCAGCA GCCATTACTG TCTTAGAAGC AGCACAGAAA
    ATTGCCAAAG GTGAACCAGG TCTTGGAGAG AAAGAGGATA CAGCATCTGT GTTCATGGAG
    ATGATCTGTC CTTCAGGAAC CAATCCAGAC GACTCTCCGC TGTACGTCCT GTGCTGTAAC
    GACACCTGCA GCTTCACCTG GACTGGAGCA GAACACATCA ACCAGGACAT CTTTGAGTGC
    AGAACTTGTG GCCTGCTGGA GTCGCTGTGC TGCTGCACTG AATGTGCTAG AGTCTGTCAC
    AAGGGGCACG ACTGCAAACT GAAGAGGACG TCACCAACAG CTTACTGTGA CTGCTGGGAG
    AAGTGCAAAT GCAAGACTCT CATCGCAGGC CAGAAAGCGG CACGTCTGGA TCTGCTCTAC
    AGGCTGCTGA CCACCACTAA TCTAGTGACC ACTCCTAACA GCAGGGGAGA GCACATCTTG
    CTGTTTCTGG TGCAGACTGT TGCAAGGCAG AGTGTCGAAC ACTGTCAGTA TCGACCTCCT
    CGCATTCGAG AGGACAGGAA CCGGAAAGCT GCCAGTGCTG AAGACTCTGA TATGCCAGAT
    CATGACCTGG AACCTCCACG CTTTGCTCAG CTGGCTCTGG AGCGAGTGTT GCAGGACTGG
    AACGCACTGA AGTCAATGAT AATGTTCGGC TCTCAGGAAA ATAAAGATCC GTTAAGCGCT
    AGCAGCAGAA TCGCTCACCT TTTACCGGAG GAGCAGATGT ACCTCAACCA GCAGAGTGGC
    ACCATCAGAC TGGACTGTTT CACACACTGC CTTATTGTCA AGTGTGCACC AGACATCACT
    TTCATTGACA CACTGTTAGG GACCCTGGTG AAGGAGCTGC AGAATAAATA CACCCCCGGC
    CGGCGGGAAG AGGCCATCAA TGTCACACGG AGGTTCTTGC GCTCTGTGGC CAGAGTGTTT
    GTCATCCTCA GCGTGGAGAT GGCATCTTCC AAGAAAAAGA ACAACTTCAT CCCTCAGCCT
    ATTGGGAAGT GCAGGCGTGT GTTCCAGGCA CTGTTGCCGT ACGCAGTGGA GGAGCTCTGC
    AATGTGGCCG AGTCTCTGAT CGTGCCGGTG CGTATGGGCA TCGCTCGGCC CACAGCTCCC
    TTCACTCTGG CCAGTACCAG CATTGATGCA GTGCAGGGCA GTGAGGAGCT CTTCTCTGTG
    GAGCCTCTGC CACCTCGACC CTCCCCGGAC CAGTCCAGCA GCTCCAGTCA GTCTGCCTCT
    TCATACATCA TCAGGAACCC GCAGCCCCGC CGCAGCAGCC AGTCTCAGAC AGCCCGCGGA
    CGGGACGAGG AGCAGGATGA TATCGTGTCT GCTGACGTAG AAGAGGTGGA GGTGGTGGAG
    GGTGTTGCAG GTGAGGAGGA TCACCATGAT GACCAGGAGG AGCAGGGAGA GGAGAACGCA
    GAGGCTGAAG GACAGCATGA TGAGCACGAT GAGGATGGGA GCGACATGGA GCTGGATTTA
    TTGGCTGCCG CTGAGACCGA GAGTGACAGT GAGAGTAACC ATAGCAACCA GGACAATGCG
    AGTGGCCGCA GGAGTGTTGT GACGGCAGCC ACTGCTGGAT CTGAAGCAGG TGCCAGCAGT
    GTTCCTGCTT TCTTCTCCGA GGACGACTCC CAGTCGAATG ACTCCAGCGA CTCGGACAGC
    AGCAGCAGCC AGAGTGACGA TGTGGATCAG GAGACGTTTC TGCTGGACGA GCCTCTTGAG
    AGAACCACCG GCTCAGCACA TGCTAACAGT GCAGCCCAGG CCCCACGCTC CATGCAGTGG
    GCTGTTCGCA CTACACCCAG CCAGCGCTCT GGAGGTGGCG CCCCATCCAG CTCGTCCGCT
    CCTGCTGCGA GCTCCACTGG CCTCATCTAC ATCGACCCGT CAAACCTGCG CCGCAGCAGC
    GCCATCAGCA CCAGCGCAGC CGCTGCCGCA GCAGCCCTGG AGGCCAGTAA CTCCAGCAGT
    TATCTGACGT CTGCCAGCAG CCTGGCTCGA GCATACAGCA TCGTCATCCG CCAGATCTCT
    GACCTCATGA GTCTCATTCC CAAATACAAC CACCTGGTCT ACTCGCAGTA CCCCGCTGCT
    GTCAAACTCA CCTATCAGGA TGCTGTCAAT CTGCAGAACT TTGTGGAGGA CAAGTTGATT
    CCCACCTGGA ACTGGATGGT GTCCATCATG GACTCCACTG AAGCGCAGCT GCGTTACGGC
    TCGGCTCTGT CTTCAGCCGG GGATCCTGGT CATCCCAGCC ACCCGCTACA CGCATCCCAG
    CATGCCGGCC GCAGAGAGCG CATGACCGCT CGAGAGGAGG CCAGTCTGCG CACTCTAGAA
    GGACGAAGGC GTGCTGCTAC GCTTCTGACG GCACGTCAGG GCATGATGTC AGCGCGAGGA
    GATTTCCTGA ACTACGCTCT GTCTCTGATG CGTTCCCATA ATGACGAGCA TTCAGACGTT
    CTGCCTGTGT TAGATGTCTG TTCGCTCAAA CACGTGGCTT ACGTCTTCCA GGCGCTCATC
    TACTGGATTA AAGCCATGAA TCTGCAGACC ACGCTCGACA CCACTCAGAT TGACAGGAAG
    AGGAATCGTG AGCTGCTGGA GCTCGGTCTG GACAATGAAG ACTCTGAGCA CGAGAACGAT
    GAGGACACCA ATCAGAGCTC TACACTGCAG GATAAGGACG AGGAGCCTGT GCCTGCAGAG
    ACTGGACAAC ACCATCCGTT TTTCCGCCGC TCGGACTCCA TGACCTTCCT GGGGTGCATC
    CCTCCAAACC CTTTTGAAGT TCCTCTAGCA GAGGCCATTC CTCTGGCTGA CCAGCCGCAT
    CTCCTGCAGC CCAATGCCAG AAAGGAGGAT TTGTTTGGAC GGCCTAGTCA AGGTCTATAT
    TCGTCCTCAT ATATGGCCAG TAAAGGCCTC ACCGATCTGA CTGTGGACAT GAATTGCTTG
    CAGATTCTAC CCACAAAGAT GTCGTATTCG GCGAACATGA AGAACGTGAT GAGTATGGAG
    TCCCGGCAGA GGGGTGGCGA GGAGCAGCCG GTGGCGGAGC AGGAGATGGA TGTGTCTAAA
    CCCGGCCCTT CACCACACGA TCTGGCGGCC CAGTTAAAGA GCAGCCTGCT GGCAGAGATT
    GGCCTGACTG AGAGTGATGG CCCACCGCTG CCCACGTTTA TTCCACACTG TAGTTTTATG
    GGGATGGTGA TCTCTCATGA CATGCTGCTG GGCCGCTGGC GGCTTTCTCT GGAGCTGTTT
    GGCCGTGTTT TCATGGAGGA CGTTGGAGCA GAACCAGGAT CTATCCTGAC AGAGCTGGGG
    GGTTTTGAGG TAAAGGAGTC CAAGTTTCGT CGTGAGATGG AGAAGCTGCG TAACCTTCAG
    TCGCGTGATC TGGCCCTGGA GGTGGACCGA GACCGTGAGC AGCTCATCCA GCAGACCATG
    CGGCAGCTGA ACGCTCACTT CGGCCGCCGC TGTACCACCA CACCCATGGC CGTGCACCGC
    GTAAAGGTGA CATTCAAGGA TGAGCCTGGA GAGGGCAGTG GTGTCGCTCG CAGCTTCTAC
    ACCGCCATCG CTCAGGCCTT CTTGTCCAAC GACAAGCTGC CCAATCTGGA CTGCGTGCAG
    AGCGTCAGCA AGGGGATGCA GGCTAGCAAT CTTATGCAGC GGCTGAGGAA CCGAGATCGT
    GAGCGTGAGA GGAGGAGCGG AGGACTGAGA GCTGCATCCA GACGAGATCG TGACAGCCTA
    AAGAGACTTT TTGCTGTGTT TGACTGCAGG GACTCGCGGA GGCAGTTGTC CATCGACACC
    CGGCCGTTCA GACCTGCATC AGAAGGAAAC CCCAGTGATG AACCAGAACC GCTGCCGGCT
    CACAGACAGG CTCTGGGAGA GAGACTTTAC CCACGAGTTC ATGCCATGCA ACCGGCGTTT
    GCTAGTAAAA TCACAGGGAT GCTGCTGGAA CTGTCTCCCG CTCAGCTGCT GCTACTCCTG
    GCCAGCGAAG ACTCGCTCAG AGCCCGAGTG GAGGAGGCCA TGGAGCTGCT CATAACTCAT
    GGACGGGAAA ATGGTGCTGA CAGTATACTA GACCTCGGCC TCCTCGACAC TCCTGAGAAA
    GCACAACAGG AGAACCGAAA GAGGCATGGT TCGACTCGCA GTGTGGTGGA CATGGAGCTG
    GATGACCCTG ATGATGGGGA TGATAACGCT CCTCTGTTTT ACCAGCCTGG AAAGAGGGGT
    TTCTACTCAC CACGTCCCGG CAAGAACACT GAGGCCAGGC TCAACTGTTT CCGTAACATC
    GGCAGGATAC TGGGTTTGTG TCTGCTACAG AATGAGCTTT GCCCAATCAC TCTCAACAGA
    CACGTCATCA AGGTGCTGCT CGGCAGAAAG GTGAACTGGC ACGACTTTGC GTTCTTCGAC
    CCGGTAATGT ACGAGAGTTT GCGGCAGCTC ATCCGTCACT CTCAGACTGA AGAGGCGGAG
    GCTGTGTTTG CGGCGATGGA CCTGGCCTTC GCTATCGACC TCTGCAAGGA GGAGGGAGCA
    GGACAGGTGG AGCTGCTGTC CGGTGGAGTC AACATGCCGG TGACGCCTCT GAATGTGTAC
    GAGTACGTGA GGAGATACGC AGAGCACCGG ATGCTGGTGG TGGCAGAACA GCCTCTGCAT
    GCCATGCGGA AAGGTCTTCT GGATGTGCTT CCTAAGAACG CTCTGGAAGA TCTGACAGCG
    GAGGATTTCA GACTGCTGGT CAACGGCTGT GGAGAAGTCA ACGTCCAGAT GCTCATTAGT
    TTCACTTCAT TCAATGATGA GTCAGGTGAG AATGCAGAGA AGCTGCTGCA GTTCAAGCGC
    TGGTTTTGGT CCATCGTTGA GAAGATGAGC ATGACGGAAA GGCAGGACCT GGTGTACTTC
    TGGACGTCCA GCCCGTCTCT GCCGGCCAGT GAAGAAGGTT TCCAGCCGAT GCCCTCCATC
    ACCATCCGCC CGCCGGACGA CCAGCACCTG CCCACAGCCA ACACCTGCAT CTCTCGCCTC
    TACGTGCCAC TTTACTCTTC CAAACAGATC CTCAAACAGA AACTTTTACT AGCCATCAAG
    ACCAAGAACT TCGGTTTTGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149208
    Clone ID Related Accession (Same CDS sequence) XM_021466630.1
    Accession Version XM_021466630.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGG
    AACCCGCAGC CCCGCCGCAG CAGCCAGTCT CAGACAGCCC GCGGACGGGA CGAGGAGCAG
    GATGATATCG TGTCTGCTGA CGTAGAAGAG GTGGAGGTGG TGGAGGGTGT TGCAGGTGAG
    GAGGATCACC ATGATGACCA GGAGGAGCAG GGAGAGGAGA ACGCAGAGGC TGAAGGACAG
    CATGATGAGC ACGATGAGGA TGGGAGCGAC ATGGAGCTGG ATTTATTGGC TGCCGCTGAG
    ACCGAGAGTG ACAGTGAGAG TAACCATAGC AACCAGGACA ATGCGAGTGG CCGCAGGAGT
    GTTGTGACGG CAGCCACTGC TGGATCTGAA GCAGGTGCCA GCAGTGTTCC TGCTTTCTTC
    TCCGAGGACG ACTCCCAGTC GAATGACTCC AGCGACTCGG ACAGCAGCAG CAGCCAGAGT
    GACGATGTGG ATCAGGAGAC GTTTCTGCTG GACGAGCCTC TTGAGAGAAC CACCGGCTCA
    GCACATGCTA ACAGTGCAGC CCAGGCCCCA CGCTCCATGC AGTGGGCTGT TCGCACTACA
    CCCAGCCAGC GCTCTGGAGG TGGCGCCCCA TCCAGCTCGT CCGCTCCTGC TGCGAGCTCC
    ACTGGCCTCA TCTACATCGA CCCGTCAAAC CTGCGCCGCA GCAGCGCCAT CAGCACCAGC
    GCAGCCGCTG CCGCAGCAGC CCTGGAGGCC AGTAACTCCA GCAGTTATCT GACGTCTGCC
    AGCAGCCTGG CTCGAGCATA CAGCATCGTC ATCCGCCAGA TCTCTGACCT CATGAGTCTC
    ATTCCCAAAT ACAACCACCT GGTCTACTCG CAGTACCCCG CTGCTGTCAA ACTCACCTAT
    CAGGATGCTG TCAATCTGCA GAACTTTGTG GAGGACAAGT TGATTCCCAC CTGGAACTGG
    ATGGTGTCCA TCATGGACTC CACTGAAGCG CAGCTGCGTT ACGGCTCGGC TCTGTCTTCA
    GCCGGGGATC CTGGTCATCC CAGCCACCCG CTACACGCAT CCCAGCATGC CGGCCGCAGA
    GAGCGCATGA CCGCTCGAGA GGAGGCCAGT CTGCGCACTC TAGAAGGACG AAGGCGTGCT
    GCTACGCTTC TGACGGCACG TCAGGGCATG ATGTCAGCGC GAGGAGATTT CCTGAACTAC
    GCTCTGTCTC TGATGCGTTC CCATAATGAC GAGCATTCAG ACGTTCTGCC TGTGTTAGAT
    GTCTGTTCGC TCAAACACGT GGCTTACGTC TTCCAGGCGC TCATCTACTG GATTAAAGCC
    ATGAATCTGC AGACCACGCT CGACACCACT CAGATTGACA GGAAGAGGAA TCGTGAGCTG
    CTGGAGCTCG GTCTGGACAA TGAAGACTCT GAGCACGAGA ACGATGAGGA CACCAATCAG
    AGCTCTACAC TGCAGGATAA GGACGAGGAG CCTGTGCCTG CAGAGACTGG ACAACACCAT
    CCGTTTTTCC GCCGCTCGGA CTCCATGACC TTCCTGGGGT GCATCCCTCC AAACCCTTTT
    GAAGTTCCTC TAGCAGAGGC CATTCCTCTG GCTGACCAGC CGCATCTCCT GCAGCCCAAT
    GCCAGAAAGG AGGATTTGTT TGGACGGCCT AGTCAAGGTC TATATTCGTC CTCATATATG
    GCCAGTAAAG GCCTCACCGA TCTGACTGTG GACATGAATT GCTTGCAGAT TCTACCCACA
    AAGATGTCGT ATTCGGCGAA CATGAAGAAC GTGATGAGTA TGGAGTCCCG GCAGAGGGGT
    GGCGAGGAGC AGCCGGTGGC GGAGCAGGAG ATGGATGTGT CTAAACCCGG CCCTTCACCA
    CACGATCTGG CGGCCCAGTT AAAGAGCAGC CTGCTGGCAG AGATTGGCCT GACTGAGAGT
    GATGGCCCAC CGCTGCCCAC GTTTATTCCA CACTGTAGTT TTATGGGGAT GGTGATCTCT
    CATGACATGC TGCTGGGCCG CTGGCGGCTT TCTCTGGAGC TGTTTGGCCG TGTTTTCATG
    GAGGACGTTG GAGCAGAACC AGGATCTATC CTGACAGAGC TGGGGGGTTT TGAGGTAAAG
    GAGTCCAAGT TTCGTCGTGA GATGGAGAAG CTGCGTAACC TTCAGTCGCG TGATCTGGCC
    CTGGAGGTGG ACCGAGACCG TGAGCAGCTC ATCCAGCAGA CCATGCGGCA GCTGAACGCT
    CACTTCGGCC GCCGCTGTAC CACCACACCC ATGGCCGTGC ACCGCGTAAA GGTGACATTC
    AAGGATGAGC CTGGAGAGGG CAGTGGTGTC GCTCGCAGCT TCTACACCGC CATCGCTCAG
    GCCTTCTTGT CCAACGACAA GCTGCCCAAT CTGGACTGCG TGCAGAGCGT CAGCAAGGGG
    ATGCAGGCTA GCAATCTTAT GCAGCGGCTG AGGAACCGAG ATCGTGAGCG TGAGAGGAGG
    AGCGGAGGAC TGAGAGCTGC ATCCAGACGA GATCGTGACA GCCTAAAGAG ACTTTTTGCT
    GTGTTTGACT GCAGGGACTC GCGGAGGCAG TTGTCCATCG ACACCCGGCC GTTCAGACCT
    GCATCAGAAG GAAACCCCAG TGATGAACCA GAACCGCTGC CGGCTCACAG ACAGGCTCTG
    GGAGAGAGAC TTTACCCACG AGTTCATGCC ATGCAACCGG CGTTTGCTAG TAAAATCACA
    GGGATGCTGC TGGAACTGTC TCCCGCTCAG CTGCTGCTAC TCCTGGCCAG CGAAGACTCG
    CTCAGAGCCC GAGTGGAGGA GGCCATGGAG CTGCTCATAA CTCATGGACG GGAAAATGGT
    GCTGACAGTA TACTAGACCT CGGCCTCCTC GACACTCCTG AGAAAGCACA ACAGGAGAAC
    CGAAAGAGGC ATGGTTCGAC TCGCAGTGTG GTGGACATGG AGCTGGATGA CCCTGATGAT
    GGGGATGATA ACGCTCCTCT GTTTTACCAG CCTGGAAAGA GGGGTTTCTA CTCACCACGT
    CCCGGCAAGA ACACTGAGGC CAGGCTCAAC TGTTTCCGTA ACATCGGCAG GATACTGGGT
    TTGTGTCTGC TACAGAATGA GCTTTGCCCA ATCACTCTCA ACAGACACGT CATCAAGGTG
    CTGCTCGGCA GAAAGGTGAA CTGGCACGAC TTTGCGTTCT TCGACCCGGT AATGTACGAG
    AGTTTGCGGC AGCTCATCCG TCACTCTCAG ACTGAAGAGG CGGAGGCTGT GTTTGCGGCG
    ATGGACCTGG CCTTCGCTAT CGACCTCTGC AAGGAGGAGG GAGCAGGACA GGTGGAGCTG
    CTGTCCGGTG GAGTCAACAT GCCGGTGACG CCTCTGAATG TGTACGAGTA CGTGAGGAGA
    TACGCAGAGC ACCGGATGCT GGTGGTGGCA GAACAGCCTC TGCATGCCAT GCGGAAAGGT
    CTTCTGGATG TGCTTCCTAA GAACGCTCTG GAAGATCTGA CAGCGGAGGA TTTCAGACTG
    CTGGTCAACG GCTGTGGAGA AGTCAACGTC CAGATGCTCA TTAGTTTCAC TTCATTCAAT
    GATGAGTCAG GTGAGAATGC AGAGAAGCTG CTGCAGTTCA AGCGCTGGTT TTGGTCCATC
    GTTGAGAAGA TGAGCATGAC GGAAAGGCAG GACCTGGTGT ACTTCTGGAC GTCCAGCCCG
    TCTCTGCCGG CCAGTGAAGA AGGTTTCCAG CCGATGCCCT CCATCACCAT CCGCCCGCCG
    GACGACCAGC ACCTGCCCAC AGCCAACACC TGCATCTCTC GCCTCTACGT GCCACTTTAC
    TCTTCCAAAC AGATCCTCAA ACAGAAACTT TTACTAGCCA TCAAGACCAA GAACTTCGGT
    TTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322305.1
    CDS173..8641
    Translation

    Target ORF information:

    RefSeq Version XM_021466630.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466630.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGG
    AACCCGCAGC CCCGCCGCAG CAGCCAGTCT CAGACAGCCC GCGGACGGGA CGAGGAGCAG
    GATGATATCG TGTCTGCTGA CGTAGAAGAG GTGGAGGTGG TGGAGGGTGT TGCAGGTGAG
    GAGGATCACC ATGATGACCA GGAGGAGCAG GGAGAGGAGA ACGCAGAGGC TGAAGGACAG
    CATGATGAGC ACGATGAGGA TGGGAGCGAC ATGGAGCTGG ATTTATTGGC TGCCGCTGAG
    ACCGAGAGTG ACAGTGAGAG TAACCATAGC AACCAGGACA ATGCGAGTGG CCGCAGGAGT
    GTTGTGACGG CAGCCACTGC TGGATCTGAA GCAGGTGCCA GCAGTGTTCC TGCTTTCTTC
    TCCGAGGACG ACTCCCAGTC GAATGACTCC AGCGACTCGG ACAGCAGCAG CAGCCAGAGT
    GACGATGTGG ATCAGGAGAC GTTTCTGCTG GACGAGCCTC TTGAGAGAAC CACCGGCTCA
    GCACATGCTA ACAGTGCAGC CCAGGCCCCA CGCTCCATGC AGTGGGCTGT TCGCACTACA
    CCCAGCCAGC GCTCTGGAGG TGGCGCCCCA TCCAGCTCGT CCGCTCCTGC TGCGAGCTCC
    ACTGGCCTCA TCTACATCGA CCCGTCAAAC CTGCGCCGCA GCAGCGCCAT CAGCACCAGC
    GCAGCCGCTG CCGCAGCAGC CCTGGAGGCC AGTAACTCCA GCAGTTATCT GACGTCTGCC
    AGCAGCCTGG CTCGAGCATA CAGCATCGTC ATCCGCCAGA TCTCTGACCT CATGAGTCTC
    ATTCCCAAAT ACAACCACCT GGTCTACTCG CAGTACCCCG CTGCTGTCAA ACTCACCTAT
    CAGGATGCTG TCAATCTGCA GAACTTTGTG GAGGACAAGT TGATTCCCAC CTGGAACTGG
    ATGGTGTCCA TCATGGACTC CACTGAAGCG CAGCTGCGTT ACGGCTCGGC TCTGTCTTCA
    GCCGGGGATC CTGGTCATCC CAGCCACCCG CTACACGCAT CCCAGCATGC CGGCCGCAGA
    GAGCGCATGA CCGCTCGAGA GGAGGCCAGT CTGCGCACTC TAGAAGGACG AAGGCGTGCT
    GCTACGCTTC TGACGGCACG TCAGGGCATG ATGTCAGCGC GAGGAGATTT CCTGAACTAC
    GCTCTGTCTC TGATGCGTTC CCATAATGAC GAGCATTCAG ACGTTCTGCC TGTGTTAGAT
    GTCTGTTCGC TCAAACACGT GGCTTACGTC TTCCAGGCGC TCATCTACTG GATTAAAGCC
    ATGAATCTGC AGACCACGCT CGACACCACT CAGATTGACA GGAAGAGGAA TCGTGAGCTG
    CTGGAGCTCG GTCTGGACAA TGAAGACTCT GAGCACGAGA ACGATGAGGA CACCAATCAG
    AGCTCTACAC TGCAGGATAA GGACGAGGAG CCTGTGCCTG CAGAGACTGG ACAACACCAT
    CCGTTTTTCC GCCGCTCGGA CTCCATGACC TTCCTGGGGT GCATCCCTCC AAACCCTTTT
    GAAGTTCCTC TAGCAGAGGC CATTCCTCTG GCTGACCAGC CGCATCTCCT GCAGCCCAAT
    GCCAGAAAGG AGGATTTGTT TGGACGGCCT AGTCAAGGTC TATATTCGTC CTCATATATG
    GCCAGTAAAG GCCTCACCGA TCTGACTGTG GACATGAATT GCTTGCAGAT TCTACCCACA
    AAGATGTCGT ATTCGGCGAA CATGAAGAAC GTGATGAGTA TGGAGTCCCG GCAGAGGGGT
    GGCGAGGAGC AGCCGGTGGC GGAGCAGGAG ATGGATGTGT CTAAACCCGG CCCTTCACCA
    CACGATCTGG CGGCCCAGTT AAAGAGCAGC CTGCTGGCAG AGATTGGCCT GACTGAGAGT
    GATGGCCCAC CGCTGCCCAC GTTTATTCCA CACTGTAGTT TTATGGGGAT GGTGATCTCT
    CATGACATGC TGCTGGGCCG CTGGCGGCTT TCTCTGGAGC TGTTTGGCCG TGTTTTCATG
    GAGGACGTTG GAGCAGAACC AGGATCTATC CTGACAGAGC TGGGGGGTTT TGAGGTAAAG
    GAGTCCAAGT TTCGTCGTGA GATGGAGAAG CTGCGTAACC TTCAGTCGCG TGATCTGGCC
    CTGGAGGTGG ACCGAGACCG TGAGCAGCTC ATCCAGCAGA CCATGCGGCA GCTGAACGCT
    CACTTCGGCC GCCGCTGTAC CACCACACCC ATGGCCGTGC ACCGCGTAAA GGTGACATTC
    AAGGATGAGC CTGGAGAGGG CAGTGGTGTC GCTCGCAGCT TCTACACCGC CATCGCTCAG
    GCCTTCTTGT CCAACGACAA GCTGCCCAAT CTGGACTGCG TGCAGAGCGT CAGCAAGGGG
    ATGCAGGCTA GCAATCTTAT GCAGCGGCTG AGGAACCGAG ATCGTGAGCG TGAGAGGAGG
    AGCGGAGGAC TGAGAGCTGC ATCCAGACGA GATCGTGACA GCCTAAAGAG ACTTTTTGCT
    GTGTTTGACT GCAGGGACTC GCGGAGGCAG TTGTCCATCG ACACCCGGCC GTTCAGACCT
    GCATCAGAAG GAAACCCCAG TGATGAACCA GAACCGCTGC CGGCTCACAG ACAGGCTCTG
    GGAGAGAGAC TTTACCCACG AGTTCATGCC ATGCAACCGG CGTTTGCTAG TAAAATCACA
    GGGATGCTGC TGGAACTGTC TCCCGCTCAG CTGCTGCTAC TCCTGGCCAG CGAAGACTCG
    CTCAGAGCCC GAGTGGAGGA GGCCATGGAG CTGCTCATAA CTCATGGACG GGAAAATGGT
    GCTGACAGTA TACTAGACCT CGGCCTCCTC GACACTCCTG AGAAAGCACA ACAGGAGAAC
    CGAAAGAGGC ATGGTTCGAC TCGCAGTGTG GTGGACATGG AGCTGGATGA CCCTGATGAT
    GGGGATGATA ACGCTCCTCT GTTTTACCAG CCTGGAAAGA GGGGTTTCTA CTCACCACGT
    CCCGGCAAGA ACACTGAGGC CAGGCTCAAC TGTTTCCGTA ACATCGGCAG GATACTGGGT
    TTGTGTCTGC TACAGAATGA GCTTTGCCCA ATCACTCTCA ACAGACACGT CATCAAGGTG
    CTGCTCGGCA GAAAGGTGAA CTGGCACGAC TTTGCGTTCT TCGACCCGGT AATGTACGAG
    AGTTTGCGGC AGCTCATCCG TCACTCTCAG ACTGAAGAGG CGGAGGCTGT GTTTGCGGCG
    ATGGACCTGG CCTTCGCTAT CGACCTCTGC AAGGAGGAGG GAGCAGGACA GGTGGAGCTG
    CTGTCCGGTG GAGTCAACAT GCCGGTGACG CCTCTGAATG TGTACGAGTA CGTGAGGAGA
    TACGCAGAGC ACCGGATGCT GGTGGTGGCA GAACAGCCTC TGCATGCCAT GCGGAAAGGT
    CTTCTGGATG TGCTTCCTAA GAACGCTCTG GAAGATCTGA CAGCGGAGGA TTTCAGACTG
    CTGGTCAACG GCTGTGGAGA AGTCAACGTC CAGATGCTCA TTAGTTTCAC TTCATTCAAT
    GATGAGTCAG GTGAGAATGC AGAGAAGCTG CTGCAGTTCA AGCGCTGGTT TTGGTCCATC
    GTTGAGAAGA TGAGCATGAC GGAAAGGCAG GACCTGGTGT ACTTCTGGAC GTCCAGCCCG
    TCTCTGCCGG CCAGTGAAGA AGGTTTCCAG CCGATGCCCT CCATCACCAT CCGCCCGCCG
    GACGACCAGC ACCTGCCCAC AGCCAACACC TGCATCTCTC GCCTCTACGT GCCACTTTAC
    TCTTCCAAAC AGATCCTCAA ACAGAAACTT TTACTAGCCA TCAAGACCAA GAACTTCGGT
    TTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149209
    Clone ID Related Accession (Same CDS sequence) XM_021466631.1
    Accession Version XM_021466631.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8382bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGC
    TCCAGTCAGT CTGCCTCTTC ATACATCATC AGGAACCCGC AGCCCCGCCG CAGCAGCCAG
    TCTCAGACAG CCCGCGGACG GGACGAGGAG CAGGATGATA TCGTGTCTGC TGACGTAGAA
    GAGGTGGAGG TGGTGGAGGG TGTTGCAGGT GAGGAGGATC ACCATGATGA CCAGGAGGAG
    CAGGGAGAGG AGAACGCAGA GGCTGAAGGA CAGCATGATG AGCACGATGA GGATGGGAGC
    GACATGGAGC TGGATTTATT GGCTGCCGCT GAGACCGAGA GTGACAGTGA GAGTAACCAT
    AGCAACCAGG ACAATGCGAG TGGCCGCAGG AGTGTTGTGA CGGCAGCCAC TGCTGGATCT
    GAAGCAGGTG CCAGCAGTGT TCCTGCTTTC TTCTCCGAGG ACGACTCCCA GTCGAATGAC
    TCCAGCGACT CGGACAGCAG CAGCAGCCAG AGTGACGATG TGGATCAGGA GACGTTTCTG
    CTGGACGAGC CTCTTGAGAG AACCACCGGC TCAGCACATG CTAACAGTGC AGCCCAGGCC
    CCACGCTCCA TGCAGTGGGC TGTTCGCACT ACACCCAGCC AGCGCTCTGG AGGTGGCGCC
    CCATCCAGCT CGTCCGCTCC TGCTGCGAGC TCCACTGGCC TCATCTACAT CGACCCGTCA
    AACCTGCGCC GCAGCAGCGC CATCAGCACC AGCGCAGCCG CTGCCGCAGC AGCCCTGGAG
    GCCAGTAACT CCAGCAGTTA TCTGACGTCT GCCAGCAGCC TGGCTCGAGC ATACAGCATC
    GTCATCCGCC AGATCTCTGA CCTCATGAGT CTCATTCCCA AATACAACCA CCTGGTCTAC
    TCGCAGTACC CCGCTGCTGT CAAACTCACC TATCAGGATG CTGTCAATCT GCAGAACTTT
    GTGGAGGACA AGTTGATTCC CACCTGGAAC TGGATGGTGT CCATCATGGA CTCCACTGAA
    GCGCAGCTGC GTTACGGCTC GGCTCTGTCT TCAGCCGGGG ATCCTGGTCA TCCCAGCCAC
    CCGCTACACG CATCCCAGCA TGCCGGCCGC AGAGAGCGCA TGACCGCTCG AGAGGAGGCC
    AGTCTGCGCA CTCTAGAAGG ACGAAGGCGT GCTGCTACGC TTCTGACGGC ACGTCAGGGC
    ATGATGTCAG CGCGAGGAGA TTTCCTGAAC TACGCTCTGT CTCTGATGCG TTCCCATAAT
    GACGAGCATT CAGACGTTCT GCCTGTGTTA GATGTCTGTT CGCTCAAACA CGTGGCTTAC
    GTCTTCCAGG CGCTCATCTA CTGGATTAAA GCCATGAATC TGCAGACCAC GCTCGACACC
    ACTCAGATTG ACAGGAAGAG GAATCGTGAG CTGCTGGAGC TCGGTCTGGA CAATGAAGAC
    TCTGAGCACG AGAACGATGA GGACACCAAT CAGAGCTCTA CACTGCAGGA TAAGGACGAG
    GAGCCTGTGC CTGCAGAGAC TGGACAACAC CATCCGTTTT TCCGCCGCTC GGACTCCATG
    ACCTTCCTGG GGTGCATCCC TCCAAACCCT TTTGAAGTTC CTCTAGCAGA GGCCATTCCT
    CTGGCTGACC AGCCGCATCT CCTGCAGCCC AATGCCAGAA AGGAGGATTT GTTTGGACGG
    CCTAGTCAAG GTCTATATTC GTCCTCATAT ATGGCCAGTA AAGGCCTCAC CGATCTGACT
    GTGGACATGA ATTGCTTGCA GATTCTACCC ACAAAGATGT CGTATTCGGC GAACATGAAG
    AACGTGATGA GTATGGAGTC CCGGCAGAGG GGTGGCGAGG AGCAGCCGGT GGCGGAGCAG
    GAGATGGATG TGTCTAAACC CGGCCCTTCA CCACACGATC TGGCGGCCCA GTTAAAGAGC
    AGCCTGCTGG CAGAGATTGG CCTGACTGAG AGTGATGGCC CACCGCTGCC CACGTTTATT
    CCACACTGTA GTTTTATGGG GATGGTGATC TCTCATGACA TGCTGCTGGG CCGCTGGCGG
    CTTTCTCTGG AGCTGTTTGG CCGTGTTTTC ATGGAGGACG TTGGAGCAGA ACCAGGATCT
    ATCCTGACAG AGCTGGGGGG TTTTGAGGTA AAGGAGTCCA AGTTTCGTCG TGAGATGGAG
    AAGCTGCGTA ACCTTCAGTC GCGTGATCTG GCCCTGGAGG TGGACCGAGA CCGTGAGCAG
    CTCATCCAGC AGACCATGCG GCAGCTGAAC GCTCACTTCG GCCGCCGCTG TACCACCACA
    CCCATGGCCG TGCACCGCGT AAAGGTGACA TTCAAGGATG AGCCTGGAGA GGGCAGTGGT
    GTCGCTCGCA GCTTCTACAC CGCCATCGCT CAGGCCTTCT TGTCCAACGA CAAGCTGCCC
    AATCTGGACT GCGTGCAGAG CGTCAGCAAG GGGATGCAGG CTAGCAATCT TATGCAGCGG
    CTGAGGAACC GAGATCGTGA GCGTGAGAGG AGGAGCGGAG GACTGAGAGC TGCATCCAGA
    CGAGATCGTG ACAGCCTAAA GAGACTTTTT GCTGTGTTTG ACTGCAGGGA CTCGCGGAGG
    CAGTTGTCCA TCGACACCCG GCCGTTCAGA CCTGCATCAG AAGGAAACCC CAGTGATGAA
    CCAGAACCGC TGCCGGCTCA CAGACAGGCT CTGGGAGAGA GACTTTACCC ACGAGTTCAT
    GCCATGCAAC CGGCGTTTGC TAGTAAAATC ACAGGGATGC TGCTGGAACT GTCTCCCGCT
    CAGCTGCTGC TACTCCTGGC CAGCGAAGAC TCGCTCAGAG CCCGAGTGGA GGAGGCCATG
    GAGCTGCTCA TAACTCATGG ACGGGAAAAT GGTGCTGACA GTATACTAGA CCTCGGCCTC
    CTCGACACTC CTGAGAAAGC ACAACAGGAG AACCGAAAGA GGCATGGTTC GACTCGCAGT
    GTGGTGGACA TGGAGCTGGA TGACCCTGAT GATGGGGATG ATAACGCTCC TCTGTTTTAC
    CAGCCTGGAA AGAGGGGTTT CTACTCACCA CGTCCCGGCA AGAACACTGA GGCCAGGCTC
    AACTGTTTCC GTAACATCGG CAGGATACTG GGTTTGTGTC TGCTACAGAA TGAGCTTTGC
    CCAATCACTC TCAACAGACA CGTCATCAAG GTGCTGCTCG GCAGAAAGGT GAACTGGCAC
    GACTTTGCGT TCTTCGACCC GGTAATGTAC GAGAGTTTGC GGCAGCTCAT CCGTCACTCT
    CAGACTGAAG AGGCGGAGGC TGTGTTTGCG GCGATGGACC TGGCCTTCGC TATCGACCTC
    TGCAAGGAGG AGGGAGCAGG ACAGGTGGAG CTGCTGTCCG GTGGAGTCAA CATGCCGGTG
    ACGCCTCTGA ATGTGTACGA GTACGTGAGG AGATACGCAG AGCACCGGAT GCTGGTGGTG
    GCAGAACAGC CTCTGCATGC CATGCGGAAA GGTCTTCTGG ATGTGCTTCC TAAGAACGCT
    CTGGAAGATC TGACAGCGGA GGATTTCAGA CTGCTGGTCA ACGGCTGTGG AGAAGTCAAC
    GTCCAGATGC TCATTAGTTT CACTTCATTC AATGATGAGT CAGGTGAGAA TGCAGAGAAG
    CTGCTGCAGT TCAAGCGCTG GTTTTGGTCC ATCGTTGAGA AGATGAGCAT GACGGAAAGG
    CAGGACCTGG TGTACTTCTG GACGTCCAGC CCGTCTCTGC CGGCCAGTGA AGAAGGTTTC
    CAGCCGATGC CCTCCATCAC CATCCGCCCG CCGGACGACC AGCACCTGCC CACAGCCAAC
    ACCTGCATCT CTCGCCTCTA CGTGCCACTT TACTCTTCCA AACAGATCCT CAAACAGAAA
    CTTTTACTAG CCATCAAGAC CAAGAACTTC GGTTTTGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322306.1
    CDS173..8554
    Translation

    Target ORF information:

    RefSeq Version XM_021466631.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466631.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGC
    TCCAGTCAGT CTGCCTCTTC ATACATCATC AGGAACCCGC AGCCCCGCCG CAGCAGCCAG
    TCTCAGACAG CCCGCGGACG GGACGAGGAG CAGGATGATA TCGTGTCTGC TGACGTAGAA
    GAGGTGGAGG TGGTGGAGGG TGTTGCAGGT GAGGAGGATC ACCATGATGA CCAGGAGGAG
    CAGGGAGAGG AGAACGCAGA GGCTGAAGGA CAGCATGATG AGCACGATGA GGATGGGAGC
    GACATGGAGC TGGATTTATT GGCTGCCGCT GAGACCGAGA GTGACAGTGA GAGTAACCAT
    AGCAACCAGG ACAATGCGAG TGGCCGCAGG AGTGTTGTGA CGGCAGCCAC TGCTGGATCT
    GAAGCAGGTG CCAGCAGTGT TCCTGCTTTC TTCTCCGAGG ACGACTCCCA GTCGAATGAC
    TCCAGCGACT CGGACAGCAG CAGCAGCCAG AGTGACGATG TGGATCAGGA GACGTTTCTG
    CTGGACGAGC CTCTTGAGAG AACCACCGGC TCAGCACATG CTAACAGTGC AGCCCAGGCC
    CCACGCTCCA TGCAGTGGGC TGTTCGCACT ACACCCAGCC AGCGCTCTGG AGGTGGCGCC
    CCATCCAGCT CGTCCGCTCC TGCTGCGAGC TCCACTGGCC TCATCTACAT CGACCCGTCA
    AACCTGCGCC GCAGCAGCGC CATCAGCACC AGCGCAGCCG CTGCCGCAGC AGCCCTGGAG
    GCCAGTAACT CCAGCAGTTA TCTGACGTCT GCCAGCAGCC TGGCTCGAGC ATACAGCATC
    GTCATCCGCC AGATCTCTGA CCTCATGAGT CTCATTCCCA AATACAACCA CCTGGTCTAC
    TCGCAGTACC CCGCTGCTGT CAAACTCACC TATCAGGATG CTGTCAATCT GCAGAACTTT
    GTGGAGGACA AGTTGATTCC CACCTGGAAC TGGATGGTGT CCATCATGGA CTCCACTGAA
    GCGCAGCTGC GTTACGGCTC GGCTCTGTCT TCAGCCGGGG ATCCTGGTCA TCCCAGCCAC
    CCGCTACACG CATCCCAGCA TGCCGGCCGC AGAGAGCGCA TGACCGCTCG AGAGGAGGCC
    AGTCTGCGCA CTCTAGAAGG ACGAAGGCGT GCTGCTACGC TTCTGACGGC ACGTCAGGGC
    ATGATGTCAG CGCGAGGAGA TTTCCTGAAC TACGCTCTGT CTCTGATGCG TTCCCATAAT
    GACGAGCATT CAGACGTTCT GCCTGTGTTA GATGTCTGTT CGCTCAAACA CGTGGCTTAC
    GTCTTCCAGG CGCTCATCTA CTGGATTAAA GCCATGAATC TGCAGACCAC GCTCGACACC
    ACTCAGATTG ACAGGAAGAG GAATCGTGAG CTGCTGGAGC TCGGTCTGGA CAATGAAGAC
    TCTGAGCACG AGAACGATGA GGACACCAAT CAGAGCTCTA CACTGCAGGA TAAGGACGAG
    GAGCCTGTGC CTGCAGAGAC TGGACAACAC CATCCGTTTT TCCGCCGCTC GGACTCCATG
    ACCTTCCTGG GGTGCATCCC TCCAAACCCT TTTGAAGTTC CTCTAGCAGA GGCCATTCCT
    CTGGCTGACC AGCCGCATCT CCTGCAGCCC AATGCCAGAA AGGAGGATTT GTTTGGACGG
    CCTAGTCAAG GTCTATATTC GTCCTCATAT ATGGCCAGTA AAGGCCTCAC CGATCTGACT
    GTGGACATGA ATTGCTTGCA GATTCTACCC ACAAAGATGT CGTATTCGGC GAACATGAAG
    AACGTGATGA GTATGGAGTC CCGGCAGAGG GGTGGCGAGG AGCAGCCGGT GGCGGAGCAG
    GAGATGGATG TGTCTAAACC CGGCCCTTCA CCACACGATC TGGCGGCCCA GTTAAAGAGC
    AGCCTGCTGG CAGAGATTGG CCTGACTGAG AGTGATGGCC CACCGCTGCC CACGTTTATT
    CCACACTGTA GTTTTATGGG GATGGTGATC TCTCATGACA TGCTGCTGGG CCGCTGGCGG
    CTTTCTCTGG AGCTGTTTGG CCGTGTTTTC ATGGAGGACG TTGGAGCAGA ACCAGGATCT
    ATCCTGACAG AGCTGGGGGG TTTTGAGGTA AAGGAGTCCA AGTTTCGTCG TGAGATGGAG
    AAGCTGCGTA ACCTTCAGTC GCGTGATCTG GCCCTGGAGG TGGACCGAGA CCGTGAGCAG
    CTCATCCAGC AGACCATGCG GCAGCTGAAC GCTCACTTCG GCCGCCGCTG TACCACCACA
    CCCATGGCCG TGCACCGCGT AAAGGTGACA TTCAAGGATG AGCCTGGAGA GGGCAGTGGT
    GTCGCTCGCA GCTTCTACAC CGCCATCGCT CAGGCCTTCT TGTCCAACGA CAAGCTGCCC
    AATCTGGACT GCGTGCAGAG CGTCAGCAAG GGGATGCAGG CTAGCAATCT TATGCAGCGG
    CTGAGGAACC GAGATCGTGA GCGTGAGAGG AGGAGCGGAG GACTGAGAGC TGCATCCAGA
    CGAGATCGTG ACAGCCTAAA GAGACTTTTT GCTGTGTTTG ACTGCAGGGA CTCGCGGAGG
    CAGTTGTCCA TCGACACCCG GCCGTTCAGA CCTGCATCAG AAGGAAACCC CAGTGATGAA
    CCAGAACCGC TGCCGGCTCA CAGACAGGCT CTGGGAGAGA GACTTTACCC ACGAGTTCAT
    GCCATGCAAC CGGCGTTTGC TAGTAAAATC ACAGGGATGC TGCTGGAACT GTCTCCCGCT
    CAGCTGCTGC TACTCCTGGC CAGCGAAGAC TCGCTCAGAG CCCGAGTGGA GGAGGCCATG
    GAGCTGCTCA TAACTCATGG ACGGGAAAAT GGTGCTGACA GTATACTAGA CCTCGGCCTC
    CTCGACACTC CTGAGAAAGC ACAACAGGAG AACCGAAAGA GGCATGGTTC GACTCGCAGT
    GTGGTGGACA TGGAGCTGGA TGACCCTGAT GATGGGGATG ATAACGCTCC TCTGTTTTAC
    CAGCCTGGAA AGAGGGGTTT CTACTCACCA CGTCCCGGCA AGAACACTGA GGCCAGGCTC
    AACTGTTTCC GTAACATCGG CAGGATACTG GGTTTGTGTC TGCTACAGAA TGAGCTTTGC
    CCAATCACTC TCAACAGACA CGTCATCAAG GTGCTGCTCG GCAGAAAGGT GAACTGGCAC
    GACTTTGCGT TCTTCGACCC GGTAATGTAC GAGAGTTTGC GGCAGCTCAT CCGTCACTCT
    CAGACTGAAG AGGCGGAGGC TGTGTTTGCG GCGATGGACC TGGCCTTCGC TATCGACCTC
    TGCAAGGAGG AGGGAGCAGG ACAGGTGGAG CTGCTGTCCG GTGGAGTCAA CATGCCGGTG
    ACGCCTCTGA ATGTGTACGA GTACGTGAGG AGATACGCAG AGCACCGGAT GCTGGTGGTG
    GCAGAACAGC CTCTGCATGC CATGCGGAAA GGTCTTCTGG ATGTGCTTCC TAAGAACGCT
    CTGGAAGATC TGACAGCGGA GGATTTCAGA CTGCTGGTCA ACGGCTGTGG AGAAGTCAAC
    GTCCAGATGC TCATTAGTTT CACTTCATTC AATGATGAGT CAGGTGAGAA TGCAGAGAAG
    CTGCTGCAGT TCAAGCGCTG GTTTTGGTCC ATCGTTGAGA AGATGAGCAT GACGGAAAGG
    CAGGACCTGG TGTACTTCTG GACGTCCAGC CCGTCTCTGC CGGCCAGTGA AGAAGGTTTC
    CAGCCGATGC CCTCCATCAC CATCCGCCCG CCGGACGACC AGCACCTGCC CACAGCCAAC
    ACCTGCATCT CTCGCCTCTA CGTGCCACTT TACTCTTCCA AACAGATCCT CAAACAGAAA
    CTTTTACTAG CCATCAAGAC CAAGAACTTC GGTTTTGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22029
    Clone ID Related Accession (Same CDS sequence) XM_005158437.3 , XM_005158437.4
    Accession Version XM_005158437.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase UBR5 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158437.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGC
    TCCAGTCAGT CTGCCTCTTC ATACATCATC AGGAACCCGC AGCCCCGCCG CAGCAGCCAG
    TCTCAGACAG CCCGCGGACG GGACGAGGAG CAGGATGATA TCGTGTCTGC TGACGTAGAA
    GAGGTGGAGG TGGTGGAGGG TGTTGCAGGT GAGGAGGATC ACCATGATGA CCAGGAGGAG
    CAGGGAGAGG AGAACGCAGA GGCTGAAGGA CAGCATGATG AGCACGATGA GGATGGGAGC
    GACATGGAGC TGGATTTATT GGCTGCCGCT GAGACCGAGA GTGACAGTGA GAGTAACCAT
    AGCAACCAGG ACAATGCGAG TGGCCGCAGG AGTGTTGTGA CGGCAGCCAC TGCTGGATCT
    GAAGCAGGTG CCAGCAGTGT TCCTGCTTTC TTCTCCGAGG ACGACTCCCA GTCGAATGAC
    TCCAGCGACT CGGACAGCAG CAGCAGCCAG AGTGACGATG TGGATCAGGA GACGTTTCTG
    CTGGACGAGC CTCTTGAGAG AACCACCGGC TCAGCACATG CTAACAGTGC AGCCCAGGCC
    CCACGCTCCA TGCAGTGGGC TGTTCGCACT ACACCCAGCC AGCGCTCTGG AGGTGGCGCC
    CCATCCAGCT CGTCCGCTCC TGCTGCGAGC TCCACTGGCC TCATCTACAT CGACCCGTCA
    AACCTGCGCC GCAGCAGCGC CATCAGCACC AGCGCAGCCG CTGCCGCAGC AGCCCTGGAG
    GCCAGTAACT CCAGCAGTTA TCTGACGTCT GCCAGCAGCC TGGCTCGAGC ATACAGCATC
    GTCATCCGCC AGATCTCTGA CCTCATGAGT CTCATTCCCA AATACAACCA CCTGGTCTAC
    TCGCAGTACC CCGCTGCTGT CAAACTCACC TATCAGGATG CTGTCAATCT GCAGAACTTT
    GTGGAGGACA AGTTGATTCC CACCTGGAAC TGGATGGTGT CCATCATGGA CTCCACTGAA
    GCGCAGCTGC GTTACGGCTC GGCTCTGTCT TCAGCCGGGG ATCCTGGTCA TCCCAGCCAC
    CCGCTACACG CATCCCAGCA TGCCGGCCGC AGAGAGCGCA TGACCGCTCG AGAGGAGGCC
    AGTCTGCGCA CTCTAGAAGG ACGAAGGCGT GCTGCTACGC TTCTGACGGC ACGTCAGGGC
    ATGATGTCAG CGCGAGGAGA TTTCCTGAAC TACGCTCTGT CTCTGATGCG TTCCCATAAT
    GACGAGCATT CAGACGTTCT GCCTGTGTTA GATGTCTGTT CGCTCAAACA CGTGGCTTAC
    GTCTTCCAGG CGCTCATCTA CTGGATTAAA GCCATGAATC TGCAGACCAC GCTCGACACC
    ACTCAGATTG ACAGGAAGAG GAATCGTGAG CTGCTGGAGC TCGGTCTGGA CAATGAAGAC
    TCTGAGCACG AGAACGATGA GGACACCAAT CAGAGCTCTA CACTGCAGGA TAAGGACGAG
    GAGCCTGTGC CTGCAGAGAC TGGACAACAC CATCCGTTTT TCCGCCGCTC GGACTCCATG
    ACCTTCCTGG GGTGCATCCC TCCAAACCCT TTTGAAGTTC CTCTAGCAGA GGCCATTCCT
    CTGGCTGACC AGCCGCATCT CCTGCAGCCC AATGCCAGAA AGGAGGATTT GTTTGGACGG
    CCTAGTCAAG GTCTATATTC GTCCTCATAT ATGGCCAGTA AAGGCCTCAC CGATCTGACT
    GTGGACATGA ATTGCTTGCA GATTCTACCC ACAAAGATGT CGTATTCGGC GAACATGAAG
    AACGTGATGA GTATGGAGTC CCGGCAGAGG GGTGGCGAGG AGCAGCCGGT GGCGGAGCAG
    GAGATGGATG TGTCTAAACC CGGCCCTTCA CCACACGATC TGGCGGCCCA GTTAAAGAGC
    AGCCTGCTGG CAGAGATTGG CCTGACTGAG AGTGATGGCC CACCGCTGCC CACGTTTATT
    CCACACTGTA GTTTTATGGG GATGGTGATC TCTCATGACA TGCTGCTGGG CCGCTGGCGG
    CTTTCTCTGG AGCTGTTTGG CCGTGTTTTC ATGGAGGACG TTGGAGCAGA ACCAGGATCT
    ATCCTGACAG AGCTGGGGGG TTTTGAGGTA AAGGAGTCCA AGTTTCGTCG TGAGATGGAG
    AAGCTGCGTA ACCTTCAGTC GCGTGATCTG GCCCTGGAGG TGGACCGAGA CCGTGAGCAG
    CTCATCCAGC AGACCATGCG GCAGCTGAAC GCTCACTTCG GCCGCCGCTG TACCACCACA
    CCCATGGCCG TGCACCGCGT AAAGGTGACA TTCAAGGATG AGCCTGGAGA GGGCAGTGGT
    GTCGCTCGCA GCTTCTACAC CGCCATCGCT CAGGCCTTCT TGTCCAACGA CAAGCTGCCC
    AATCTGGACT GCGTGCAGAG CGTCAGCAAG GGGATGCAGG CTAGCAATCT TATGCAGCGG
    CTGAGGAACC GAGATCGTGA GCGTGAGAGG AGGAGCGGAG GACTGAGAGC TGCATCCAGA
    CGAGATCGTG ACAGGGACTC GCGGAGGCAG TTGTCCATCG ACACCCGGCC GTTCAGACCT
    GCATCAGAAG GAAACCCCAG TGATGAACCA GAACCGCTGC CGGCTCACAG ACAGGCTCTG
    GGAGAGAGAC TTTACCCACG AGTTCATGCC ATGCAACCGG CGTTTGCTAG TAAAATCACA
    GGGATGCTGC TGGAACTGTC TCCCGCTCAG CTGCTGCTAC TCCTGGCCAG CGAAGACTCG
    CTCAGAGCCC GAGTGGAGGA GGCCATGGAG CTGCTCATAA CTCATGGACG GGAAAATGGT
    GCTGACAGTA TACTAGACCT CGGCCTCCTC GACACTCCTG AGAAAGCACA ACAGGAGAAC
    CGAAAGAGGC ATGGTTCGAC TCGCAGTGTG GTGGACATGG AGCTGGATGA CCCTGATGAT
    GGGGATGATA ACGCTCCTCT GTTTTACCAG CCTGGAAAGA GGGGTTTCTA CTCACCACGT
    CCCGGCAAGA ACACTGAGGC CAGGCTCAAC TGTTTCCGTA ACATCGGCAG GATACTGGGT
    TTGTGTCTGC TACAGAATGA GCTTTGCCCA ATCACTCTCA ACAGACACGT CATCAAGGTG
    CTGCTCGGCA GAAAGGTGAA CTGGCACGAC TTTGCGTTCT TCGACCCGGT AATGTACGAG
    AGTTTGCGGC AGCTCATCCG TCACTCTCAG ACTGAAGAGG CGGAGGCTGT GTTTGCGGCG
    ATGGACCTGG CCTTCGCTAT CGACCTCTGC AAGGAGGAGG GAGCAGGACA GGTGGAGCTG
    CTGTCCGGTG GAGTCAACAT GCCGGTGACG CCTCTGAATG TGTACGAGTA CGTGAGGAGA
    TACGCAGAGC ACCGGATGCT GGTGGTGGCA GAACAGCCTC TGCATGCCAT GCGGAAAGGT
    CTTCTGGATG TGCTTCCTAA GAACGCTCTG GAAGATCTGA CAGCGGAGGA TTTCAGACTG
    CTGGTCAACG GCTGTGGAGA AGTCAACGTC CAGATGCTCA TTAGTTTCAC TTCATTCAAT
    GATGAGTCAG GTGAGAATGC AGAGAAGCTG CTGCAGTTCA AGCGCTGGTT TTGGTCCATC
    GTTGAGAAGA TGAGCATGAC GGAAAGGCAG GACCTGGTGT ACTTCTGGAC GTCCAGCCCG
    TCTCTGCCGG CCAGTGAAGA AGGTTTCCAG CCGATGCCCT CCATCACCAT CCGCCCGCCG
    GACGACCAGC ACCTGCCCAC AGCCAACACC TGCATCTCTC GCCTCTACGT GCCACTTTAC
    TCTTCCAAAC AGATCCTCAA ACAGAAACTT TTACTAGCCA TCAAGACCAA GAACTTCGGT
    TTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158494.1
    CDS234..8702
    Translation

    Target ORF information:

    RefSeq Version XM_005158437.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158437.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGC
    TCCAGTCAGT CTGCCTCTTC ATACATCATC AGGAACCCGC AGCCCCGCCG CAGCAGCCAG
    TCTCAGACAG CCCGCGGACG GGACGAGGAG CAGGATGATA TCGTGTCTGC TGACGTAGAA
    GAGGTGGAGG TGGTGGAGGG TGTTGCAGGT GAGGAGGATC ACCATGATGA CCAGGAGGAG
    CAGGGAGAGG AGAACGCAGA GGCTGAAGGA CAGCATGATG AGCACGATGA GGATGGGAGC
    GACATGGAGC TGGATTTATT GGCTGCCGCT GAGACCGAGA GTGACAGTGA GAGTAACCAT
    AGCAACCAGG ACAATGCGAG TGGCCGCAGG AGTGTTGTGA CGGCAGCCAC TGCTGGATCT
    GAAGCAGGTG CCAGCAGTGT TCCTGCTTTC TTCTCCGAGG ACGACTCCCA GTCGAATGAC
    TCCAGCGACT CGGACAGCAG CAGCAGCCAG AGTGACGATG TGGATCAGGA GACGTTTCTG
    CTGGACGAGC CTCTTGAGAG AACCACCGGC TCAGCACATG CTAACAGTGC AGCCCAGGCC
    CCACGCTCCA TGCAGTGGGC TGTTCGCACT ACACCCAGCC AGCGCTCTGG AGGTGGCGCC
    CCATCCAGCT CGTCCGCTCC TGCTGCGAGC TCCACTGGCC TCATCTACAT CGACCCGTCA
    AACCTGCGCC GCAGCAGCGC CATCAGCACC AGCGCAGCCG CTGCCGCAGC AGCCCTGGAG
    GCCAGTAACT CCAGCAGTTA TCTGACGTCT GCCAGCAGCC TGGCTCGAGC ATACAGCATC
    GTCATCCGCC AGATCTCTGA CCTCATGAGT CTCATTCCCA AATACAACCA CCTGGTCTAC
    TCGCAGTACC CCGCTGCTGT CAAACTCACC TATCAGGATG CTGTCAATCT GCAGAACTTT
    GTGGAGGACA AGTTGATTCC CACCTGGAAC TGGATGGTGT CCATCATGGA CTCCACTGAA
    GCGCAGCTGC GTTACGGCTC GGCTCTGTCT TCAGCCGGGG ATCCTGGTCA TCCCAGCCAC
    CCGCTACACG CATCCCAGCA TGCCGGCCGC AGAGAGCGCA TGACCGCTCG AGAGGAGGCC
    AGTCTGCGCA CTCTAGAAGG ACGAAGGCGT GCTGCTACGC TTCTGACGGC ACGTCAGGGC
    ATGATGTCAG CGCGAGGAGA TTTCCTGAAC TACGCTCTGT CTCTGATGCG TTCCCATAAT
    GACGAGCATT CAGACGTTCT GCCTGTGTTA GATGTCTGTT CGCTCAAACA CGTGGCTTAC
    GTCTTCCAGG CGCTCATCTA CTGGATTAAA GCCATGAATC TGCAGACCAC GCTCGACACC
    ACTCAGATTG ACAGGAAGAG GAATCGTGAG CTGCTGGAGC TCGGTCTGGA CAATGAAGAC
    TCTGAGCACG AGAACGATGA GGACACCAAT CAGAGCTCTA CACTGCAGGA TAAGGACGAG
    GAGCCTGTGC CTGCAGAGAC TGGACAACAC CATCCGTTTT TCCGCCGCTC GGACTCCATG
    ACCTTCCTGG GGTGCATCCC TCCAAACCCT TTTGAAGTTC CTCTAGCAGA GGCCATTCCT
    CTGGCTGACC AGCCGCATCT CCTGCAGCCC AATGCCAGAA AGGAGGATTT GTTTGGACGG
    CCTAGTCAAG GTCTATATTC GTCCTCATAT ATGGCCAGTA AAGGCCTCAC CGATCTGACT
    GTGGACATGA ATTGCTTGCA GATTCTACCC ACAAAGATGT CGTATTCGGC GAACATGAAG
    AACGTGATGA GTATGGAGTC CCGGCAGAGG GGTGGCGAGG AGCAGCCGGT GGCGGAGCAG
    GAGATGGATG TGTCTAAACC CGGCCCTTCA CCACACGATC TGGCGGCCCA GTTAAAGAGC
    AGCCTGCTGG CAGAGATTGG CCTGACTGAG AGTGATGGCC CACCGCTGCC CACGTTTATT
    CCACACTGTA GTTTTATGGG GATGGTGATC TCTCATGACA TGCTGCTGGG CCGCTGGCGG
    CTTTCTCTGG AGCTGTTTGG CCGTGTTTTC ATGGAGGACG TTGGAGCAGA ACCAGGATCT
    ATCCTGACAG AGCTGGGGGG TTTTGAGGTA AAGGAGTCCA AGTTTCGTCG TGAGATGGAG
    AAGCTGCGTA ACCTTCAGTC GCGTGATCTG GCCCTGGAGG TGGACCGAGA CCGTGAGCAG
    CTCATCCAGC AGACCATGCG GCAGCTGAAC GCTCACTTCG GCCGCCGCTG TACCACCACA
    CCCATGGCCG TGCACCGCGT AAAGGTGACA TTCAAGGATG AGCCTGGAGA GGGCAGTGGT
    GTCGCTCGCA GCTTCTACAC CGCCATCGCT CAGGCCTTCT TGTCCAACGA CAAGCTGCCC
    AATCTGGACT GCGTGCAGAG CGTCAGCAAG GGGATGCAGG CTAGCAATCT TATGCAGCGG
    CTGAGGAACC GAGATCGTGA GCGTGAGAGG AGGAGCGGAG GACTGAGAGC TGCATCCAGA
    CGAGATCGTG ACAGGGACTC GCGGAGGCAG TTGTCCATCG ACACCCGGCC GTTCAGACCT
    GCATCAGAAG GAAACCCCAG TGATGAACCA GAACCGCTGC CGGCTCACAG ACAGGCTCTG
    GGAGAGAGAC TTTACCCACG AGTTCATGCC ATGCAACCGG CGTTTGCTAG TAAAATCACA
    GGGATGCTGC TGGAACTGTC TCCCGCTCAG CTGCTGCTAC TCCTGGCCAG CGAAGACTCG
    CTCAGAGCCC GAGTGGAGGA GGCCATGGAG CTGCTCATAA CTCATGGACG GGAAAATGGT
    GCTGACAGTA TACTAGACCT CGGCCTCCTC GACACTCCTG AGAAAGCACA ACAGGAGAAC
    CGAAAGAGGC ATGGTTCGAC TCGCAGTGTG GTGGACATGG AGCTGGATGA CCCTGATGAT
    GGGGATGATA ACGCTCCTCT GTTTTACCAG CCTGGAAAGA GGGGTTTCTA CTCACCACGT
    CCCGGCAAGA ACACTGAGGC CAGGCTCAAC TGTTTCCGTA ACATCGGCAG GATACTGGGT
    TTGTGTCTGC TACAGAATGA GCTTTGCCCA ATCACTCTCA ACAGACACGT CATCAAGGTG
    CTGCTCGGCA GAAAGGTGAA CTGGCACGAC TTTGCGTTCT TCGACCCGGT AATGTACGAG
    AGTTTGCGGC AGCTCATCCG TCACTCTCAG ACTGAAGAGG CGGAGGCTGT GTTTGCGGCG
    ATGGACCTGG CCTTCGCTAT CGACCTCTGC AAGGAGGAGG GAGCAGGACA GGTGGAGCTG
    CTGTCCGGTG GAGTCAACAT GCCGGTGACG CCTCTGAATG TGTACGAGTA CGTGAGGAGA
    TACGCAGAGC ACCGGATGCT GGTGGTGGCA GAACAGCCTC TGCATGCCAT GCGGAAAGGT
    CTTCTGGATG TGCTTCCTAA GAACGCTCTG GAAGATCTGA CAGCGGAGGA TTTCAGACTG
    CTGGTCAACG GCTGTGGAGA AGTCAACGTC CAGATGCTCA TTAGTTTCAC TTCATTCAAT
    GATGAGTCAG GTGAGAATGC AGAGAAGCTG CTGCAGTTCA AGCGCTGGTT TTGGTCCATC
    GTTGAGAAGA TGAGCATGAC GGAAAGGCAG GACCTGGTGT ACTTCTGGAC GTCCAGCCCG
    TCTCTGCCGG CCAGTGAAGA AGGTTTCCAG CCGATGCCCT CCATCACCAT CCGCCCGCCG
    GACGACCAGC ACCTGCCCAC AGCCAACACC TGCATCTCTC GCCTCTACGT GCCACTTTAC
    TCTTCCAAAC AGATCCTCAA ACAGAAACTT TTACTAGCCA TCAAGACCAA GAACTTCGGT
    TTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22029
    Clone ID Related Accession (Same CDS sequence) XM_005158437.3 , XM_005158437.4
    Accession Version XM_005158437.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8469bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158437.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGC
    TCCAGTCAGT CTGCCTCTTC ATACATCATC AGGAACCCGC AGCCCCGCCG CAGCAGCCAG
    TCTCAGACAG CCCGCGGACG GGACGAGGAG CAGGATGATA TCGTGTCTGC TGACGTAGAA
    GAGGTGGAGG TGGTGGAGGG TGTTGCAGGT GAGGAGGATC ACCATGATGA CCAGGAGGAG
    CAGGGAGAGG AGAACGCAGA GGCTGAAGGA CAGCATGATG AGCACGATGA GGATGGGAGC
    GACATGGAGC TGGATTTATT GGCTGCCGCT GAGACCGAGA GTGACAGTGA GAGTAACCAT
    AGCAACCAGG ACAATGCGAG TGGCCGCAGG AGTGTTGTGA CGGCAGCCAC TGCTGGATCT
    GAAGCAGGTG CCAGCAGTGT TCCTGCTTTC TTCTCCGAGG ACGACTCCCA GTCGAATGAC
    TCCAGCGACT CGGACAGCAG CAGCAGCCAG AGTGACGATG TGGATCAGGA GACGTTTCTG
    CTGGACGAGC CTCTTGAGAG AACCACCGGC TCAGCACATG CTAACAGTGC AGCCCAGGCC
    CCACGCTCCA TGCAGTGGGC TGTTCGCACT ACACCCAGCC AGCGCTCTGG AGGTGGCGCC
    CCATCCAGCT CGTCCGCTCC TGCTGCGAGC TCCACTGGCC TCATCTACAT CGACCCGTCA
    AACCTGCGCC GCAGCAGCGC CATCAGCACC AGCGCAGCCG CTGCCGCAGC AGCCCTGGAG
    GCCAGTAACT CCAGCAGTTA TCTGACGTCT GCCAGCAGCC TGGCTCGAGC ATACAGCATC
    GTCATCCGCC AGATCTCTGA CCTCATGAGT CTCATTCCCA AATACAACCA CCTGGTCTAC
    TCGCAGTACC CCGCTGCTGT CAAACTCACC TATCAGGATG CTGTCAATCT GCAGAACTTT
    GTGGAGGACA AGTTGATTCC CACCTGGAAC TGGATGGTGT CCATCATGGA CTCCACTGAA
    GCGCAGCTGC GTTACGGCTC GGCTCTGTCT TCAGCCGGGG ATCCTGGTCA TCCCAGCCAC
    CCGCTACACG CATCCCAGCA TGCCGGCCGC AGAGAGCGCA TGACCGCTCG AGAGGAGGCC
    AGTCTGCGCA CTCTAGAAGG ACGAAGGCGT GCTGCTACGC TTCTGACGGC ACGTCAGGGC
    ATGATGTCAG CGCGAGGAGA TTTCCTGAAC TACGCTCTGT CTCTGATGCG TTCCCATAAT
    GACGAGCATT CAGACGTTCT GCCTGTGTTA GATGTCTGTT CGCTCAAACA CGTGGCTTAC
    GTCTTCCAGG CGCTCATCTA CTGGATTAAA GCCATGAATC TGCAGACCAC GCTCGACACC
    ACTCAGATTG ACAGGAAGAG GAATCGTGAG CTGCTGGAGC TCGGTCTGGA CAATGAAGAC
    TCTGAGCACG AGAACGATGA GGACACCAAT CAGAGCTCTA CACTGCAGGA TAAGGACGAG
    GAGCCTGTGC CTGCAGAGAC TGGACAACAC CATCCGTTTT TCCGCCGCTC GGACTCCATG
    ACCTTCCTGG GGTGCATCCC TCCAAACCCT TTTGAAGTTC CTCTAGCAGA GGCCATTCCT
    CTGGCTGACC AGCCGCATCT CCTGCAGCCC AATGCCAGAA AGGAGGATTT GTTTGGACGG
    CCTAGTCAAG GTCTATATTC GTCCTCATAT ATGGCCAGTA AAGGCCTCAC CGATCTGACT
    GTGGACATGA ATTGCTTGCA GATTCTACCC ACAAAGATGT CGTATTCGGC GAACATGAAG
    AACGTGATGA GTATGGAGTC CCGGCAGAGG GGTGGCGAGG AGCAGCCGGT GGCGGAGCAG
    GAGATGGATG TGTCTAAACC CGGCCCTTCA CCACACGATC TGGCGGCCCA GTTAAAGAGC
    AGCCTGCTGG CAGAGATTGG CCTGACTGAG AGTGATGGCC CACCGCTGCC CACGTTTATT
    CCACACTGTA GTTTTATGGG GATGGTGATC TCTCATGACA TGCTGCTGGG CCGCTGGCGG
    CTTTCTCTGG AGCTGTTTGG CCGTGTTTTC ATGGAGGACG TTGGAGCAGA ACCAGGATCT
    ATCCTGACAG AGCTGGGGGG TTTTGAGGTA AAGGAGTCCA AGTTTCGTCG TGAGATGGAG
    AAGCTGCGTA ACCTTCAGTC GCGTGATCTG GCCCTGGAGG TGGACCGAGA CCGTGAGCAG
    CTCATCCAGC AGACCATGCG GCAGCTGAAC GCTCACTTCG GCCGCCGCTG TACCACCACA
    CCCATGGCCG TGCACCGCGT AAAGGTGACA TTCAAGGATG AGCCTGGAGA GGGCAGTGGT
    GTCGCTCGCA GCTTCTACAC CGCCATCGCT CAGGCCTTCT TGTCCAACGA CAAGCTGCCC
    AATCTGGACT GCGTGCAGAG CGTCAGCAAG GGGATGCAGG CTAGCAATCT TATGCAGCGG
    CTGAGGAACC GAGATCGTGA GCGTGAGAGG AGGAGCGGAG GACTGAGAGC TGCATCCAGA
    CGAGATCGTG ACAGGGACTC GCGGAGGCAG TTGTCCATCG ACACCCGGCC GTTCAGACCT
    GCATCAGAAG GAAACCCCAG TGATGAACCA GAACCGCTGC CGGCTCACAG ACAGGCTCTG
    GGAGAGAGAC TTTACCCACG AGTTCATGCC ATGCAACCGG CGTTTGCTAG TAAAATCACA
    GGGATGCTGC TGGAACTGTC TCCCGCTCAG CTGCTGCTAC TCCTGGCCAG CGAAGACTCG
    CTCAGAGCCC GAGTGGAGGA GGCCATGGAG CTGCTCATAA CTCATGGACG GGAAAATGGT
    GCTGACAGTA TACTAGACCT CGGCCTCCTC GACACTCCTG AGAAAGCACA ACAGGAGAAC
    CGAAAGAGGC ATGGTTCGAC TCGCAGTGTG GTGGACATGG AGCTGGATGA CCCTGATGAT
    GGGGATGATA ACGCTCCTCT GTTTTACCAG CCTGGAAAGA GGGGTTTCTA CTCACCACGT
    CCCGGCAAGA ACACTGAGGC CAGGCTCAAC TGTTTCCGTA ACATCGGCAG GATACTGGGT
    TTGTGTCTGC TACAGAATGA GCTTTGCCCA ATCACTCTCA ACAGACACGT CATCAAGGTG
    CTGCTCGGCA GAAAGGTGAA CTGGCACGAC TTTGCGTTCT TCGACCCGGT AATGTACGAG
    AGTTTGCGGC AGCTCATCCG TCACTCTCAG ACTGAAGAGG CGGAGGCTGT GTTTGCGGCG
    ATGGACCTGG CCTTCGCTAT CGACCTCTGC AAGGAGGAGG GAGCAGGACA GGTGGAGCTG
    CTGTCCGGTG GAGTCAACAT GCCGGTGACG CCTCTGAATG TGTACGAGTA CGTGAGGAGA
    TACGCAGAGC ACCGGATGCT GGTGGTGGCA GAACAGCCTC TGCATGCCAT GCGGAAAGGT
    CTTCTGGATG TGCTTCCTAA GAACGCTCTG GAAGATCTGA CAGCGGAGGA TTTCAGACTG
    CTGGTCAACG GCTGTGGAGA AGTCAACGTC CAGATGCTCA TTAGTTTCAC TTCATTCAAT
    GATGAGTCAG GTGAGAATGC AGAGAAGCTG CTGCAGTTCA AGCGCTGGTT TTGGTCCATC
    GTTGAGAAGA TGAGCATGAC GGAAAGGCAG GACCTGGTGT ACTTCTGGAC GTCCAGCCCG
    TCTCTGCCGG CCAGTGAAGA AGGTTTCCAG CCGATGCCCT CCATCACCAT CCGCCCGCCG
    GACGACCAGC ACCTGCCCAC AGCCAACACC TGCATCTCTC GCCTCTACGT GCCACTTTAC
    TCTTCCAAAC AGATCCTCAA ACAGAAACTT TTACTAGCCA TCAAGACCAA GAACTTCGGT
    TTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158494.1
    CDS173..8641
    Translation

    Target ORF information:

    RefSeq Version XM_005158437.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158437.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGC
    TCCAGTCAGT CTGCCTCTTC ATACATCATC AGGAACCCGC AGCCCCGCCG CAGCAGCCAG
    TCTCAGACAG CCCGCGGACG GGACGAGGAG CAGGATGATA TCGTGTCTGC TGACGTAGAA
    GAGGTGGAGG TGGTGGAGGG TGTTGCAGGT GAGGAGGATC ACCATGATGA CCAGGAGGAG
    CAGGGAGAGG AGAACGCAGA GGCTGAAGGA CAGCATGATG AGCACGATGA GGATGGGAGC
    GACATGGAGC TGGATTTATT GGCTGCCGCT GAGACCGAGA GTGACAGTGA GAGTAACCAT
    AGCAACCAGG ACAATGCGAG TGGCCGCAGG AGTGTTGTGA CGGCAGCCAC TGCTGGATCT
    GAAGCAGGTG CCAGCAGTGT TCCTGCTTTC TTCTCCGAGG ACGACTCCCA GTCGAATGAC
    TCCAGCGACT CGGACAGCAG CAGCAGCCAG AGTGACGATG TGGATCAGGA GACGTTTCTG
    CTGGACGAGC CTCTTGAGAG AACCACCGGC TCAGCACATG CTAACAGTGC AGCCCAGGCC
    CCACGCTCCA TGCAGTGGGC TGTTCGCACT ACACCCAGCC AGCGCTCTGG AGGTGGCGCC
    CCATCCAGCT CGTCCGCTCC TGCTGCGAGC TCCACTGGCC TCATCTACAT CGACCCGTCA
    AACCTGCGCC GCAGCAGCGC CATCAGCACC AGCGCAGCCG CTGCCGCAGC AGCCCTGGAG
    GCCAGTAACT CCAGCAGTTA TCTGACGTCT GCCAGCAGCC TGGCTCGAGC ATACAGCATC
    GTCATCCGCC AGATCTCTGA CCTCATGAGT CTCATTCCCA AATACAACCA CCTGGTCTAC
    TCGCAGTACC CCGCTGCTGT CAAACTCACC TATCAGGATG CTGTCAATCT GCAGAACTTT
    GTGGAGGACA AGTTGATTCC CACCTGGAAC TGGATGGTGT CCATCATGGA CTCCACTGAA
    GCGCAGCTGC GTTACGGCTC GGCTCTGTCT TCAGCCGGGG ATCCTGGTCA TCCCAGCCAC
    CCGCTACACG CATCCCAGCA TGCCGGCCGC AGAGAGCGCA TGACCGCTCG AGAGGAGGCC
    AGTCTGCGCA CTCTAGAAGG ACGAAGGCGT GCTGCTACGC TTCTGACGGC ACGTCAGGGC
    ATGATGTCAG CGCGAGGAGA TTTCCTGAAC TACGCTCTGT CTCTGATGCG TTCCCATAAT
    GACGAGCATT CAGACGTTCT GCCTGTGTTA GATGTCTGTT CGCTCAAACA CGTGGCTTAC
    GTCTTCCAGG CGCTCATCTA CTGGATTAAA GCCATGAATC TGCAGACCAC GCTCGACACC
    ACTCAGATTG ACAGGAAGAG GAATCGTGAG CTGCTGGAGC TCGGTCTGGA CAATGAAGAC
    TCTGAGCACG AGAACGATGA GGACACCAAT CAGAGCTCTA CACTGCAGGA TAAGGACGAG
    GAGCCTGTGC CTGCAGAGAC TGGACAACAC CATCCGTTTT TCCGCCGCTC GGACTCCATG
    ACCTTCCTGG GGTGCATCCC TCCAAACCCT TTTGAAGTTC CTCTAGCAGA GGCCATTCCT
    CTGGCTGACC AGCCGCATCT CCTGCAGCCC AATGCCAGAA AGGAGGATTT GTTTGGACGG
    CCTAGTCAAG GTCTATATTC GTCCTCATAT ATGGCCAGTA AAGGCCTCAC CGATCTGACT
    GTGGACATGA ATTGCTTGCA GATTCTACCC ACAAAGATGT CGTATTCGGC GAACATGAAG
    AACGTGATGA GTATGGAGTC CCGGCAGAGG GGTGGCGAGG AGCAGCCGGT GGCGGAGCAG
    GAGATGGATG TGTCTAAACC CGGCCCTTCA CCACACGATC TGGCGGCCCA GTTAAAGAGC
    AGCCTGCTGG CAGAGATTGG CCTGACTGAG AGTGATGGCC CACCGCTGCC CACGTTTATT
    CCACACTGTA GTTTTATGGG GATGGTGATC TCTCATGACA TGCTGCTGGG CCGCTGGCGG
    CTTTCTCTGG AGCTGTTTGG CCGTGTTTTC ATGGAGGACG TTGGAGCAGA ACCAGGATCT
    ATCCTGACAG AGCTGGGGGG TTTTGAGGTA AAGGAGTCCA AGTTTCGTCG TGAGATGGAG
    AAGCTGCGTA ACCTTCAGTC GCGTGATCTG GCCCTGGAGG TGGACCGAGA CCGTGAGCAG
    CTCATCCAGC AGACCATGCG GCAGCTGAAC GCTCACTTCG GCCGCCGCTG TACCACCACA
    CCCATGGCCG TGCACCGCGT AAAGGTGACA TTCAAGGATG AGCCTGGAGA GGGCAGTGGT
    GTCGCTCGCA GCTTCTACAC CGCCATCGCT CAGGCCTTCT TGTCCAACGA CAAGCTGCCC
    AATCTGGACT GCGTGCAGAG CGTCAGCAAG GGGATGCAGG CTAGCAATCT TATGCAGCGG
    CTGAGGAACC GAGATCGTGA GCGTGAGAGG AGGAGCGGAG GACTGAGAGC TGCATCCAGA
    CGAGATCGTG ACAGGGACTC GCGGAGGCAG TTGTCCATCG ACACCCGGCC GTTCAGACCT
    GCATCAGAAG GAAACCCCAG TGATGAACCA GAACCGCTGC CGGCTCACAG ACAGGCTCTG
    GGAGAGAGAC TTTACCCACG AGTTCATGCC ATGCAACCGG CGTTTGCTAG TAAAATCACA
    GGGATGCTGC TGGAACTGTC TCCCGCTCAG CTGCTGCTAC TCCTGGCCAG CGAAGACTCG
    CTCAGAGCCC GAGTGGAGGA GGCCATGGAG CTGCTCATAA CTCATGGACG GGAAAATGGT
    GCTGACAGTA TACTAGACCT CGGCCTCCTC GACACTCCTG AGAAAGCACA ACAGGAGAAC
    CGAAAGAGGC ATGGTTCGAC TCGCAGTGTG GTGGACATGG AGCTGGATGA CCCTGATGAT
    GGGGATGATA ACGCTCCTCT GTTTTACCAG CCTGGAAAGA GGGGTTTCTA CTCACCACGT
    CCCGGCAAGA ACACTGAGGC CAGGCTCAAC TGTTTCCGTA ACATCGGCAG GATACTGGGT
    TTGTGTCTGC TACAGAATGA GCTTTGCCCA ATCACTCTCA ACAGACACGT CATCAAGGTG
    CTGCTCGGCA GAAAGGTGAA CTGGCACGAC TTTGCGTTCT TCGACCCGGT AATGTACGAG
    AGTTTGCGGC AGCTCATCCG TCACTCTCAG ACTGAAGAGG CGGAGGCTGT GTTTGCGGCG
    ATGGACCTGG CCTTCGCTAT CGACCTCTGC AAGGAGGAGG GAGCAGGACA GGTGGAGCTG
    CTGTCCGGTG GAGTCAACAT GCCGGTGACG CCTCTGAATG TGTACGAGTA CGTGAGGAGA
    TACGCAGAGC ACCGGATGCT GGTGGTGGCA GAACAGCCTC TGCATGCCAT GCGGAAAGGT
    CTTCTGGATG TGCTTCCTAA GAACGCTCTG GAAGATCTGA CAGCGGAGGA TTTCAGACTG
    CTGGTCAACG GCTGTGGAGA AGTCAACGTC CAGATGCTCA TTAGTTTCAC TTCATTCAAT
    GATGAGTCAG GTGAGAATGC AGAGAAGCTG CTGCAGTTCA AGCGCTGGTT TTGGTCCATC
    GTTGAGAAGA TGAGCATGAC GGAAAGGCAG GACCTGGTGT ACTTCTGGAC GTCCAGCCCG
    TCTCTGCCGG CCAGTGAAGA AGGTTTCCAG CCGATGCCCT CCATCACCAT CCGCCCGCCG
    GACGACCAGC ACCTGCCCAC AGCCAACACC TGCATCTCTC GCCTCTACGT GCCACTTTAC
    TCTTCCAAAC AGATCCTCAA ACAGAAACTT TTACTAGCCA TCAAGACCAA GAACTTCGGT
    TTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22030
    Clone ID Related Accession (Same CDS sequence) XM_005158438.3
    Accession Version XM_005158438.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase UBR5 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158438.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GCAGAGCGGT CTGAAAGAAA CACGTTCGCT
    GAGAGGCTTT CTGCAGTGGA GGCCATTGCC AATGCCATAT CAGTGGTGTC GAGCAACAGC
    TCCGGGAACC GGACAGGATC ATCCAGCAGC AGGGGCTTGC GTTTGAGAGA GATGATGCGG
    CGCTCACTCC GTGCTGCAGG TTTAGGCCGA CATGAATCCG GACCTTCCTC CAGCGACCAT
    CAAGACCCCG TTTCACCCCC GATAGCACCT CCTAGCTGGG TACCAGACCC TCCACCGATG
    GATCCTGACG GTGACATTGA TTTCATCTTG GCCCCTGCAG TGGGCTCCCT CACCACTGCT
    TCCACAGGCA CCAGCCAGGG ACCCAGCACT TCCACCATCC CAGGACCCTC CTCTGAACCA
    TCAGTAGTGG AATCTAAAGA CCGTAAGGCG AACGCACACC TGATACTGAA GCTGATGTGT
    GACAGCATCG TCCTGCGGCC TCATCTAAGA GAACTGCTCT CTGCCAAGGA TGCTCGTGGA
    ATGACTCCAT TCATGTTAGC AGTGAGTGGC AGAGCTTACC CAGCAGCCAT TACTGTCTTA
    GAAGCAGCAC AGAAAATTGC CAAAGGTGAA CCAGGTCTTG GAGAGAAAGA GGATACAGCA
    TCTGTGTTCA TGGAGATGAT CTGTCCTTCA GGAACCAATC CAGACGACTC TCCGCTGTAC
    GTCCTGTGCT GTAACGACAC CTGCAGCTTC ACCTGGACTG GAGCAGAACA CATCAACCAG
    GACATCTTTG AGTGCAGAAC TTGTGGCCTG CTGGAGTCGC TGTGCTGCTG CACTGAATGT
    GCTAGAGTCT GTCACAAGGG GCACGACTGC AAACTGAAGA GGACGTCACC AACAGCTTAC
    TGTGACTGCT GGGAGAAGTG CAAATGCAAG ACTCTCATCG CAGGCCAGAA AGCGGCACGT
    CTGGATCTGC TCTACAGGCT GCTGACCACC ACTAATCTAG TGACCACTCC TAACAGCAGG
    GGAGAGCACA TCTTGCTGTT TCTGGTGCAG ACTGTTGCAA GGCAGAGTGT CGAACACTGT
    CAGTATCGAC CTCCTCGCAT TCGAGAGGAC AGGAACCGGA AAGCTGCCAG TGCTGAAGAC
    TCTGATATGC CAGATCATGA CCTGGAACCT CCACGCTTTG CTCAGCTGGC TCTGGAGCGA
    GTGTTGCAGG ACTGGAACGC ACTGAAGTCA ATGATAATGT TCGGCTCTCA GGAAAATAAA
    GATCCGTTAA GCGCTAGCAG CAGAATCGCT CACCTTTTAC CGGAGGAGCA GATGTACCTC
    AACCAGCAGA GTGGCACCAT CAGACTGGAC TGTTTCACAC ACTGCCTTAT TGTCAAGTGT
    GCACCAGACA TCACTTTCAT TGACACACTG TTAGGGACCC TGGTGAAGGA GCTGCAGAAT
    AAATACACCC CCGGCCGGCG GGAAGAGGCC ATCAATGTCA CACGGAGGTT CTTGCGCTCT
    GTGGCCAGAG TGTTTGTCAT CCTCAGCGTG GAGATGGCAT CTTCCAAGAA AAAGAACAAC
    TTCATCCCTC AGCCTATTGG GAAGTGCAGG CGTGTGTTCC AGGCACTGTT GCCGTACGCA
    GTGGAGGAGC TCTGCAATGT GGCCGAGTCT CTGATCGTGC CGGTGCGTAT GGGCATCGCT
    CGGCCCACAG CTCCCTTCAC TCTGGCCAGT ACCAGCATTG ATGCAGTGCA GGGCAGTGAG
    GAGCTCTTCT CTGTGGAGCC TCTGCCACCT CGACCCTCCC CGGACCAGTC CAGCAGCTCC
    AGTCAGTCTG CCTCTTCATA CATCATCAGG AACCCGCAGC CCCGCCGCAG CAGCCAGTCT
    CAGACAGCCC GCGGACGGGA CGAGGAGCAG GATGATATCG TGTCTGCTGA CGTAGAAGAG
    GTGGAGGTGG TGGAGGGTGT TGCAGGTGAG GAGGATCACC ATGATGACCA GGAGGAGCAG
    GGAGAGGAGA ACGCAGAGGC TGAAGGACAG CATGATGAGC ACGATGAGGA TGGGAGCGAC
    ATGGAGCTGG ATTTATTGGC TGCCGCTGAG ACCGAGAGTG ACAGTGAGAG TAACCATAGC
    AACCAGGACA ATGCGAGTGG CCGCAGGAGT GTTGTGACGG CAGCCACTGC TGGATCTGAA
    GCAGGTGCCA GCAGTGTTCC TGCTTTCTTC TCCGAGGACG ACTCCCAGTC GAATGACTCC
    AGCGACTCGG ACAGCAGCAG CAGCCAGAGT GACGATGTGG ATCAGGAGAC GTTTCTGCTG
    GACGAGCCTC TTGAGAGAAC CACCGGCTCA GCACATGCTA ACAGTGCAGC CCAGGCCCCA
    CGCTCCATGC AGTGGGCTGT TCGCACTACA CCCAGCCAGC GCTCTGGAGG TGGCGCCCCA
    TCCAGCTCGT CCGCTCCTGC TGCGAGCTCC ACTGGCCTCA TCTACATCGA CCCGTCAAAC
    CTGCGCCGCA GCAGCGCCAT CAGCACCAGC GCAGCCGCTG CCGCAGCAGC CCTGGAGGCC
    AGTAACTCCA GCAGTTATCT GACGTCTGCC AGCAGCCTGG CTCGAGCATA CAGCATCGTC
    ATCCGCCAGA TCTCTGACCT CATGAGTCTC ATTCCCAAAT ACAACCACCT GGTCTACTCG
    CAGTACCCCG CTGCTGTCAA ACTCACCTAT CAGGATGCTG TCAATCTGCA GAACTTTGTG
    GAGGACAAGT TGATTCCCAC CTGGAACTGG ATGGTGTCCA TCATGGACTC CACTGAAGCG
    CAGCTGCGTT ACGGCTCGGC TCTGTCTTCA GCCGGGGATC CTGGTCATCC CAGCCACCCG
    CTACACGCAT CCCAGCATGC CGGCCGCAGA GAGCGCATGA CCGCTCGAGA GGAGGCCAGT
    CTGCGCACTC TAGAAGGACG AAGGCGTGCT GCTACGCTTC TGACGGCACG TCAGGGCATG
    ATGTCAGCGC GAGGAGATTT CCTGAACTAC GCTCTGTCTC TGATGCGTTC CCATAATGAC
    GAGCATTCAG ACGTTCTGCC TGTGTTAGAT GTCTGTTCGC TCAAACACGT GGCTTACGTC
    TTCCAGGCGC TCATCTACTG GATTAAAGCC ATGAATCTGC AGACCACGCT CGACACCACT
    CAGATTGACA GGAAGAGGAA TCGTGAGCTG CTGGAGCTCG GTCTGGACAA TGAAGACTCT
    GAGCACGAGA ACGATGAGGA CACCAATCAG AGCTCTACAC TGCAGGATAA GGACGAGGAG
    CCTGTGCCTG CAGAGACTGG ACAACACCAT CCGTTTTTCC GCCGCTCGGA CTCCATGACC
    TTCCTGGGGT GCATCCCTCC AAACCCTTTT GAAGTTCCTC TAGCAGAGGC CATTCCTCTG
    GCTGACCAGC CGCATCTCCT GCAGCCCAAT GCCAGAAAGG AGGATTTGTT TGGACGGCCT
    AGTCAAGGTC TATATTCGTC CTCATATATG GCCAGTAAAG GCCTCACCGA TCTGACTGTG
    GACATGAATT GCTTGCAGAT TCTACCCACA AAGATGTCGT ATTCGGCGAA CATGAAGAAC
    GTGATGAGTA TGGAGTCCCG GCAGAGGGGT GGCGAGGAGC AGCCGGTGGC GGAGCAGGAG
    ATGGATGTGT CTAAACCCGG CCCTTCACCA CACGATCTGG CGGCCCAGTT AAAGAGCAGC
    CTGCTGGCAG AGATTGGCCT GACTGAGAGT GATGGCCCAC CGCTGCCCAC GTTTATTCCA
    CACTGTAGTT TTATGGGGAT GGTGATCTCT CATGACATGC TGCTGGGCCG CTGGCGGCTT
    TCTCTGGAGC TGTTTGGCCG TGTTTTCATG GAGGACGTTG GAGCAGAACC AGGATCTATC
    CTGACAGAGC TGGGGGGTTT TGAGGTAAAG GAGTCCAAGT TTCGTCGTGA GATGGAGAAG
    CTGCGTAACC TTCAGTCGCG TGATCTGGCC CTGGAGGTGG ACCGAGACCG TGAGCAGCTC
    ATCCAGCAGA CCATGCGGCA GCTGAACGCT CACTTCGGCC GCCGCTGTAC CACCACACCC
    ATGGCCGTGC ACCGCGTAAA GGTGACATTC AAGGATGAGC CTGGAGAGGG CAGTGGTGTC
    GCTCGCAGCT TCTACACCGC CATCGCTCAG GCCTTCTTGT CCAACGACAA GCTGCCCAAT
    CTGGACTGCG TGCAGAGCGT CAGCAAGGGG ATGCAGGCTA GCAATCTTAT GCAGCGGCTG
    AGGAACCGAG ATCGTGAGCG TGAGAGGAGG AGCGGAGGAC TGAGAGCTGC ATCCAGACGA
    GATCGTGACA GGGACTCGCG GAGGCAGTTG TCCATCGACA CCCGGCCGTT CAGACCTGCA
    TCAGAAGGAA ACCCCAGTGA TGAACCAGAA CCGCTGCCGG CTCACAGACA GGCTCTGGGA
    GAGAGACTTT ACCCACGAGT TCATGCCATG CAACCGGCGT TTGCTAGTAA AATCACAGGG
    ATGCTGCTGG AACTGTCTCC CGCTCAGCTG CTGCTACTCC TGGCCAGCGA AGACTCGCTC
    AGAGCCCGAG TGGAGGAGGC CATGGAGCTG CTCATAACTC ATGGACGGGA AAATGGTGCT
    GACAGTATAC TAGACCTCGG CCTCCTCGAC ACTCCTGAGA AAGCACAACA GGAGAACCGA
    AAGAGGCATG GTTCGACTCG CAGTGTGGTG GACATGGAGC TGGATGACCC TGATGATGGG
    GATGATAACG CTCCTCTGTT TTACCAGCCT GGAAAGAGGG GTTTCTACTC ACCACGTCCC
    GGCAAGAACA CTGAGGCCAG GCTCAACTGT TTCCGTAACA TCGGCAGGAT ACTGGGTTTG
    TGTCTGCTAC AGAATGAGCT TTGCCCAATC ACTCTCAACA GACACGTCAT CAAGGTGCTG
    CTCGGCAGAA AGGTGAACTG GCACGACTTT GCGTTCTTCG ACCCGGTAAT GTACGAGAGT
    TTGCGGCAGC TCATCCGTCA CTCTCAGACT GAAGAGGCGG AGGCTGTGTT TGCGGCGATG
    GACCTGGCCT TCGCTATCGA CCTCTGCAAG GAGGAGGGAG CAGGACAGGT GGAGCTGCTG
    TCCGGTGGAG TCAACATGCC GGTGACGCCT CTGAATGTGT ACGAGTACGT GAGGAGATAC
    GCAGAGCACC GGATGCTGGT GGTGGCAGAA CAGCCTCTGC ATGCCATGCG GAAAGGTCTT
    CTGGATGTGC TTCCTAAGAA CGCTCTGGAA GATCTGACAG CGGAGGATTT CAGACTGCTG
    GTCAACGGCT GTGGAGAAGT CAACGTCCAG ATGCTCATTA GTTTCACTTC ATTCAATGAT
    GAGTCAGGTG AGAATGCAGA GAAGCTGCTG CAGTTCAAGC GCTGGTTTTG GTCCATCGTT
    GAGAAGATGA GCATGACGGA AAGGCAGGAC CTGGTGTACT TCTGGACGTC CAGCCCGTCT
    CTGCCGGCCA GTGAAGAAGG TTTCCAGCCG ATGCCCTCCA TCACCATCCG CCCGCCGGAC
    GACCAGCACC TGCCCACAGC CAACACCTGC ATCTCTCGCC TCTACGTGCC ACTTTACTCT
    TCCAAACAGA TCCTCAAACA GAAACTTTTA CTAGCCATCA AGACCAAGAA CTTCGGTTTT
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158495.1
    CDS235..8700
    Translation

    Target ORF information:

    RefSeq Version XM_005158438.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158438.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GCAGAGCGGT CTGAAAGAAA CACGTTCGCT
    GAGAGGCTTT CTGCAGTGGA GGCCATTGCC AATGCCATAT CAGTGGTGTC GAGCAACAGC
    TCCGGGAACC GGACAGGATC ATCCAGCAGC AGGGGCTTGC GTTTGAGAGA GATGATGCGG
    CGCTCACTCC GTGCTGCAGG TTTAGGCCGA CATGAATCCG GACCTTCCTC CAGCGACCAT
    CAAGACCCCG TTTCACCCCC GATAGCACCT CCTAGCTGGG TACCAGACCC TCCACCGATG
    GATCCTGACG GTGACATTGA TTTCATCTTG GCCCCTGCAG TGGGCTCCCT CACCACTGCT
    TCCACAGGCA CCAGCCAGGG ACCCAGCACT TCCACCATCC CAGGACCCTC CTCTGAACCA
    TCAGTAGTGG AATCTAAAGA CCGTAAGGCG AACGCACACC TGATACTGAA GCTGATGTGT
    GACAGCATCG TCCTGCGGCC TCATCTAAGA GAACTGCTCT CTGCCAAGGA TGCTCGTGGA
    ATGACTCCAT TCATGTTAGC AGTGAGTGGC AGAGCTTACC CAGCAGCCAT TACTGTCTTA
    GAAGCAGCAC AGAAAATTGC CAAAGGTGAA CCAGGTCTTG GAGAGAAAGA GGATACAGCA
    TCTGTGTTCA TGGAGATGAT CTGTCCTTCA GGAACCAATC CAGACGACTC TCCGCTGTAC
    GTCCTGTGCT GTAACGACAC CTGCAGCTTC ACCTGGACTG GAGCAGAACA CATCAACCAG
    GACATCTTTG AGTGCAGAAC TTGTGGCCTG CTGGAGTCGC TGTGCTGCTG CACTGAATGT
    GCTAGAGTCT GTCACAAGGG GCACGACTGC AAACTGAAGA GGACGTCACC AACAGCTTAC
    TGTGACTGCT GGGAGAAGTG CAAATGCAAG ACTCTCATCG CAGGCCAGAA AGCGGCACGT
    CTGGATCTGC TCTACAGGCT GCTGACCACC ACTAATCTAG TGACCACTCC TAACAGCAGG
    GGAGAGCACA TCTTGCTGTT TCTGGTGCAG ACTGTTGCAA GGCAGAGTGT CGAACACTGT
    CAGTATCGAC CTCCTCGCAT TCGAGAGGAC AGGAACCGGA AAGCTGCCAG TGCTGAAGAC
    TCTGATATGC CAGATCATGA CCTGGAACCT CCACGCTTTG CTCAGCTGGC TCTGGAGCGA
    GTGTTGCAGG ACTGGAACGC ACTGAAGTCA ATGATAATGT TCGGCTCTCA GGAAAATAAA
    GATCCGTTAA GCGCTAGCAG CAGAATCGCT CACCTTTTAC CGGAGGAGCA GATGTACCTC
    AACCAGCAGA GTGGCACCAT CAGACTGGAC TGTTTCACAC ACTGCCTTAT TGTCAAGTGT
    GCACCAGACA TCACTTTCAT TGACACACTG TTAGGGACCC TGGTGAAGGA GCTGCAGAAT
    AAATACACCC CCGGCCGGCG GGAAGAGGCC ATCAATGTCA CACGGAGGTT CTTGCGCTCT
    GTGGCCAGAG TGTTTGTCAT CCTCAGCGTG GAGATGGCAT CTTCCAAGAA AAAGAACAAC
    TTCATCCCTC AGCCTATTGG GAAGTGCAGG CGTGTGTTCC AGGCACTGTT GCCGTACGCA
    GTGGAGGAGC TCTGCAATGT GGCCGAGTCT CTGATCGTGC CGGTGCGTAT GGGCATCGCT
    CGGCCCACAG CTCCCTTCAC TCTGGCCAGT ACCAGCATTG ATGCAGTGCA GGGCAGTGAG
    GAGCTCTTCT CTGTGGAGCC TCTGCCACCT CGACCCTCCC CGGACCAGTC CAGCAGCTCC
    AGTCAGTCTG CCTCTTCATA CATCATCAGG AACCCGCAGC CCCGCCGCAG CAGCCAGTCT
    CAGACAGCCC GCGGACGGGA CGAGGAGCAG GATGATATCG TGTCTGCTGA CGTAGAAGAG
    GTGGAGGTGG TGGAGGGTGT TGCAGGTGAG GAGGATCACC ATGATGACCA GGAGGAGCAG
    GGAGAGGAGA ACGCAGAGGC TGAAGGACAG CATGATGAGC ACGATGAGGA TGGGAGCGAC
    ATGGAGCTGG ATTTATTGGC TGCCGCTGAG ACCGAGAGTG ACAGTGAGAG TAACCATAGC
    AACCAGGACA ATGCGAGTGG CCGCAGGAGT GTTGTGACGG CAGCCACTGC TGGATCTGAA
    GCAGGTGCCA GCAGTGTTCC TGCTTTCTTC TCCGAGGACG ACTCCCAGTC GAATGACTCC
    AGCGACTCGG ACAGCAGCAG CAGCCAGAGT GACGATGTGG ATCAGGAGAC GTTTCTGCTG
    GACGAGCCTC TTGAGAGAAC CACCGGCTCA GCACATGCTA ACAGTGCAGC CCAGGCCCCA
    CGCTCCATGC AGTGGGCTGT TCGCACTACA CCCAGCCAGC GCTCTGGAGG TGGCGCCCCA
    TCCAGCTCGT CCGCTCCTGC TGCGAGCTCC ACTGGCCTCA TCTACATCGA CCCGTCAAAC
    CTGCGCCGCA GCAGCGCCAT CAGCACCAGC GCAGCCGCTG CCGCAGCAGC CCTGGAGGCC
    AGTAACTCCA GCAGTTATCT GACGTCTGCC AGCAGCCTGG CTCGAGCATA CAGCATCGTC
    ATCCGCCAGA TCTCTGACCT CATGAGTCTC ATTCCCAAAT ACAACCACCT GGTCTACTCG
    CAGTACCCCG CTGCTGTCAA ACTCACCTAT CAGGATGCTG TCAATCTGCA GAACTTTGTG
    GAGGACAAGT TGATTCCCAC CTGGAACTGG ATGGTGTCCA TCATGGACTC CACTGAAGCG
    CAGCTGCGTT ACGGCTCGGC TCTGTCTTCA GCCGGGGATC CTGGTCATCC CAGCCACCCG
    CTACACGCAT CCCAGCATGC CGGCCGCAGA GAGCGCATGA CCGCTCGAGA GGAGGCCAGT
    CTGCGCACTC TAGAAGGACG AAGGCGTGCT GCTACGCTTC TGACGGCACG TCAGGGCATG
    ATGTCAGCGC GAGGAGATTT CCTGAACTAC GCTCTGTCTC TGATGCGTTC CCATAATGAC
    GAGCATTCAG ACGTTCTGCC TGTGTTAGAT GTCTGTTCGC TCAAACACGT GGCTTACGTC
    TTCCAGGCGC TCATCTACTG GATTAAAGCC ATGAATCTGC AGACCACGCT CGACACCACT
    CAGATTGACA GGAAGAGGAA TCGTGAGCTG CTGGAGCTCG GTCTGGACAA TGAAGACTCT
    GAGCACGAGA ACGATGAGGA CACCAATCAG AGCTCTACAC TGCAGGATAA GGACGAGGAG
    CCTGTGCCTG CAGAGACTGG ACAACACCAT CCGTTTTTCC GCCGCTCGGA CTCCATGACC
    TTCCTGGGGT GCATCCCTCC AAACCCTTTT GAAGTTCCTC TAGCAGAGGC CATTCCTCTG
    GCTGACCAGC CGCATCTCCT GCAGCCCAAT GCCAGAAAGG AGGATTTGTT TGGACGGCCT
    AGTCAAGGTC TATATTCGTC CTCATATATG GCCAGTAAAG GCCTCACCGA TCTGACTGTG
    GACATGAATT GCTTGCAGAT TCTACCCACA AAGATGTCGT ATTCGGCGAA CATGAAGAAC
    GTGATGAGTA TGGAGTCCCG GCAGAGGGGT GGCGAGGAGC AGCCGGTGGC GGAGCAGGAG
    ATGGATGTGT CTAAACCCGG CCCTTCACCA CACGATCTGG CGGCCCAGTT AAAGAGCAGC
    CTGCTGGCAG AGATTGGCCT GACTGAGAGT GATGGCCCAC CGCTGCCCAC GTTTATTCCA
    CACTGTAGTT TTATGGGGAT GGTGATCTCT CATGACATGC TGCTGGGCCG CTGGCGGCTT
    TCTCTGGAGC TGTTTGGCCG TGTTTTCATG GAGGACGTTG GAGCAGAACC AGGATCTATC
    CTGACAGAGC TGGGGGGTTT TGAGGTAAAG GAGTCCAAGT TTCGTCGTGA GATGGAGAAG
    CTGCGTAACC TTCAGTCGCG TGATCTGGCC CTGGAGGTGG ACCGAGACCG TGAGCAGCTC
    ATCCAGCAGA CCATGCGGCA GCTGAACGCT CACTTCGGCC GCCGCTGTAC CACCACACCC
    ATGGCCGTGC ACCGCGTAAA GGTGACATTC AAGGATGAGC CTGGAGAGGG CAGTGGTGTC
    GCTCGCAGCT TCTACACCGC CATCGCTCAG GCCTTCTTGT CCAACGACAA GCTGCCCAAT
    CTGGACTGCG TGCAGAGCGT CAGCAAGGGG ATGCAGGCTA GCAATCTTAT GCAGCGGCTG
    AGGAACCGAG ATCGTGAGCG TGAGAGGAGG AGCGGAGGAC TGAGAGCTGC ATCCAGACGA
    GATCGTGACA GGGACTCGCG GAGGCAGTTG TCCATCGACA CCCGGCCGTT CAGACCTGCA
    TCAGAAGGAA ACCCCAGTGA TGAACCAGAA CCGCTGCCGG CTCACAGACA GGCTCTGGGA
    GAGAGACTTT ACCCACGAGT TCATGCCATG CAACCGGCGT TTGCTAGTAA AATCACAGGG
    ATGCTGCTGG AACTGTCTCC CGCTCAGCTG CTGCTACTCC TGGCCAGCGA AGACTCGCTC
    AGAGCCCGAG TGGAGGAGGC CATGGAGCTG CTCATAACTC ATGGACGGGA AAATGGTGCT
    GACAGTATAC TAGACCTCGG CCTCCTCGAC ACTCCTGAGA AAGCACAACA GGAGAACCGA
    AAGAGGCATG GTTCGACTCG CAGTGTGGTG GACATGGAGC TGGATGACCC TGATGATGGG
    GATGATAACG CTCCTCTGTT TTACCAGCCT GGAAAGAGGG GTTTCTACTC ACCACGTCCC
    GGCAAGAACA CTGAGGCCAG GCTCAACTGT TTCCGTAACA TCGGCAGGAT ACTGGGTTTG
    TGTCTGCTAC AGAATGAGCT TTGCCCAATC ACTCTCAACA GACACGTCAT CAAGGTGCTG
    CTCGGCAGAA AGGTGAACTG GCACGACTTT GCGTTCTTCG ACCCGGTAAT GTACGAGAGT
    TTGCGGCAGC TCATCCGTCA CTCTCAGACT GAAGAGGCGG AGGCTGTGTT TGCGGCGATG
    GACCTGGCCT TCGCTATCGA CCTCTGCAAG GAGGAGGGAG CAGGACAGGT GGAGCTGCTG
    TCCGGTGGAG TCAACATGCC GGTGACGCCT CTGAATGTGT ACGAGTACGT GAGGAGATAC
    GCAGAGCACC GGATGCTGGT GGTGGCAGAA CAGCCTCTGC ATGCCATGCG GAAAGGTCTT
    CTGGATGTGC TTCCTAAGAA CGCTCTGGAA GATCTGACAG CGGAGGATTT CAGACTGCTG
    GTCAACGGCT GTGGAGAAGT CAACGTCCAG ATGCTCATTA GTTTCACTTC ATTCAATGAT
    GAGTCAGGTG AGAATGCAGA GAAGCTGCTG CAGTTCAAGC GCTGGTTTTG GTCCATCGTT
    GAGAAGATGA GCATGACGGA AAGGCAGGAC CTGGTGTACT TCTGGACGTC CAGCCCGTCT
    CTGCCGGCCA GTGAAGAAGG TTTCCAGCCG ATGCCCTCCA TCACCATCCG CCCGCCGGAC
    GACCAGCACC TGCCCACAGC CAACACCTGC ATCTCTCGCC TCTACGTGCC ACTTTACTCT
    TCCAAACAGA TCCTCAAACA GAAACTTTTA CTAGCCATCA AGACCAAGAA CTTCGGTTTT
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22031
    Clone ID Related Accession (Same CDS sequence) XM_005158439.3
    Accession Version XM_005158439.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8454bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase UBR5 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158439.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGC
    TCCAGTCAGT CTGCCTCTTC ATACATCATC AGGAACCCGC AGCCCCGCCG CAGCAGCCAG
    TCTCAGACAG CCCGCGGACG GGACGAGGAG CAGGATGATA TCGTGTCTGC TGACGTAGAA
    GAGGTGGAGG TGGTGGAGGG TGTTGCAGGT GAGGAGGATC ACCATGATGA CCAGGAGGAG
    CAGGGAGAGG AGAACGCAGA GGCTGAAGGA CAGCATGATG AGCACGATGA GGATGGGAGC
    GACATGGAGC TGGATTTATT GGCTGCCGCT GAGACCGAGA GTGACAGTGA GAGTAACCAT
    AGCAACCAGG ACAATGCGAG TGGCCGCAGG AGTGTTGTGA CGGCAGCCAC TGCTGGATCT
    GAAGCAGGTG CCAGCAGTGT TCCTGCTTTC TTCTCCGAGG ACGACTCCCA GTCGAATGAC
    TCCAGCGACT CGGACAGCAG CAGCAGCCAG AGTGACGATG TGGATCAGGA GACGTTTCTG
    CTGGACGAGC CTCTTGAGAG AACCACCGGC TCAGCACATG CTAACAGTGC AGCCCAGGCC
    CCACGCTCCA TGCAGTGGGC TGTTCGCACT ACACCCAGCC AGCGCTCTGG AGGTGGCGCC
    CCATCCAGCT CGTCCGCTCC TGCTGCGAGC TCCACTGGCC TCATCTACAT CGACCCGTCA
    AACCTGCGCC GCAGCAGCGC CATCAGCACC AGCGCAGCCG CTGCCGCAGC AGCCCTGGAG
    GCCAGTAACT CCAGCAGTTA TCTGACGTCT GCCAGCAGCC TGGCTCGAGC ATACAGCATC
    GTCATCCGCC AGATCTCTGA CCTCATGAGT CTCATTCCCA AATACAACCA CCTGGTCTAC
    TCGCAGTACC CCGCTGCTGT CAAACTCACC TATCAGGATG CTGTCAATCT GCAGAACTTT
    GTGGAGGACA AGTTGATTCC CACCTGGAAC TGGATGGTGT CCATCATGGA CTCCACTGAA
    GCGCAGCTGC GTTACGGCTC GGCTCTGTCT TCAGCCGGGG ATCCTGGTCA TCCCAGCCAC
    CCGCTACACG CATCCCAGCA TGCCGGCCGC AGAGAGCGCA TGACCGCTCG AGAGGAGGCC
    AGTCTGCGCA CTCTAGAAGG ACGAAGGCGT GCTGCTACGC TTCTGACGGC ACGTCAGGGC
    ATGATGTCAG CGCGAGGAGA TTTCCTGAAC TACGCTCTGT CTCTGATGCG TTCCCATAAT
    GACGAGCATT CAGACGTTCT GCCTGTGTTA GATGTCTGTT CGCTCAAACA CGTGGCTTAC
    GTCTTCCAGG CGCTCATCTA CTGGATTAAA GCCATGAATC TGCAGACCAC GCTCGACACC
    ACTCAGATTG ACAGGAAGAG GAATCGTGAG CTGCTGGAGC TCGGTCTGGA CAATGAAGAC
    TCTGAGCACG AGAACGATGA GGACACCAAT CAGAGCTCTA CACTGCAGGA TAAGGACGAG
    GAGCCTGTGC CTGCAGAGAC TGGACAACAC CATCCGTTTT TCCGCCGCTC GGACTCCATG
    ACCTTCCTGG GGTGCATCCC TCCAAACCCT TTTGAAGTTC CTCTAGCAGA GGCCATTCCT
    CTGGCTGACC AGCCGCATCT CCTGCAGCCC AATGCCAGAA AGGAGGATTT GTTTGGACGG
    CCTAGTCAAG GTCTATATTC GTCCTCATAT ATGGCCAGTA AAGGCCTCAC CGATCTGACT
    GTGGACATGA ATTGCTTGCA GATGTCGTAT TCGGCGAACA TGAAGAACGT GATGAGTATG
    GAGTCCCGGC AGAGGGGTGG CGAGGAGCAG CCGGTGGCGG AGCAGGAGAT GGATGTGTCT
    AAACCCGGCC CTTCACCACA CGATCTGGCG GCCCAGTTAA AGAGCAGCCT GCTGGCAGAG
    ATTGGCCTGA CTGAGAGTGA TGGCCCACCG CTGCCCACGT TTATTCCACA CTGTAGTTTT
    ATGGGGATGG TGATCTCTCA TGACATGCTG CTGGGCCGCT GGCGGCTTTC TCTGGAGCTG
    TTTGGCCGTG TTTTCATGGA GGACGTTGGA GCAGAACCAG GATCTATCCT GACAGAGCTG
    GGGGGTTTTG AGGTAAAGGA GTCCAAGTTT CGTCGTGAGA TGGAGAAGCT GCGTAACCTT
    CAGTCGCGTG ATCTGGCCCT GGAGGTGGAC CGAGACCGTG AGCAGCTCAT CCAGCAGACC
    ATGCGGCAGC TGAACGCTCA CTTCGGCCGC CGCTGTACCA CCACACCCAT GGCCGTGCAC
    CGCGTAAAGG TGACATTCAA GGATGAGCCT GGAGAGGGCA GTGGTGTCGC TCGCAGCTTC
    TACACCGCCA TCGCTCAGGC CTTCTTGTCC AACGACAAGC TGCCCAATCT GGACTGCGTG
    CAGAGCGTCA GCAAGGGGAT GCAGGCTAGC AATCTTATGC AGCGGCTGAG GAACCGAGAT
    CGTGAGCGTG AGAGGAGGAG CGGAGGACTG AGAGCTGCAT CCAGACGAGA TCGTGACAGG
    GACTCGCGGA GGCAGTTGTC CATCGACACC CGGCCGTTCA GACCTGCATC AGAAGGAAAC
    CCCAGTGATG AACCAGAACC GCTGCCGGCT CACAGACAGG CTCTGGGAGA GAGACTTTAC
    CCACGAGTTC ATGCCATGCA ACCGGCGTTT GCTAGTAAAA TCACAGGGAT GCTGCTGGAA
    CTGTCTCCCG CTCAGCTGCT GCTACTCCTG GCCAGCGAAG ACTCGCTCAG AGCCCGAGTG
    GAGGAGGCCA TGGAGCTGCT CATAACTCAT GGACGGGAAA ATGGTGCTGA CAGTATACTA
    GACCTCGGCC TCCTCGACAC TCCTGAGAAA GCACAACAGG AGAACCGAAA GAGGCATGGT
    TCGACTCGCA GTGTGGTGGA CATGGAGCTG GATGACCCTG ATGATGGGGA TGATAACGCT
    CCTCTGTTTT ACCAGCCTGG AAAGAGGGGT TTCTACTCAC CACGTCCCGG CAAGAACACT
    GAGGCCAGGC TCAACTGTTT CCGTAACATC GGCAGGATAC TGGGTTTGTG TCTGCTACAG
    AATGAGCTTT GCCCAATCAC TCTCAACAGA CACGTCATCA AGGTGCTGCT CGGCAGAAAG
    GTGAACTGGC ACGACTTTGC GTTCTTCGAC CCGGTAATGT ACGAGAGTTT GCGGCAGCTC
    ATCCGTCACT CTCAGACTGA AGAGGCGGAG GCTGTGTTTG CGGCGATGGA CCTGGCCTTC
    GCTATCGACC TCTGCAAGGA GGAGGGAGCA GGACAGGTGG AGCTGCTGTC CGGTGGAGTC
    AACATGCCGG TGACGCCTCT GAATGTGTAC GAGTACGTGA GGAGATACGC AGAGCACCGG
    ATGCTGGTGG TGGCAGAACA GCCTCTGCAT GCCATGCGGA AAGGTCTTCT GGATGTGCTT
    CCTAAGAACG CTCTGGAAGA TCTGACAGCG GAGGATTTCA GACTGCTGGT CAACGGCTGT
    GGAGAAGTCA ACGTCCAGAT GCTCATTAGT TTCACTTCAT TCAATGATGA GTCAGGTGAG
    AATGCAGAGA AGCTGCTGCA GTTCAAGCGC TGGTTTTGGT CCATCGTTGA GAAGATGAGC
    ATGACGGAAA GGCAGGACCT GGTGTACTTC TGGACGTCCA GCCCGTCTCT GCCGGCCAGT
    GAAGAAGGTT TCCAGCCGAT GCCCTCCATC ACCATCCGCC CGCCGGACGA CCAGCACCTG
    CCCACAGCCA ACACCTGCAT CTCTCGCCTC TACGTGCCAC TTTACTCTTC CAAACAGATC
    CTCAAACAGA AACTTTTACT AGCCATCAAG ACCAAGAACT TCGGTTTTGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158496.1
    CDS235..8688
    Translation

    Target ORF information:

    RefSeq Version XM_005158439.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158439.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGC
    TCCAGTCAGT CTGCCTCTTC ATACATCATC AGGAACCCGC AGCCCCGCCG CAGCAGCCAG
    TCTCAGACAG CCCGCGGACG GGACGAGGAG CAGGATGATA TCGTGTCTGC TGACGTAGAA
    GAGGTGGAGG TGGTGGAGGG TGTTGCAGGT GAGGAGGATC ACCATGATGA CCAGGAGGAG
    CAGGGAGAGG AGAACGCAGA GGCTGAAGGA CAGCATGATG AGCACGATGA GGATGGGAGC
    GACATGGAGC TGGATTTATT GGCTGCCGCT GAGACCGAGA GTGACAGTGA GAGTAACCAT
    AGCAACCAGG ACAATGCGAG TGGCCGCAGG AGTGTTGTGA CGGCAGCCAC TGCTGGATCT
    GAAGCAGGTG CCAGCAGTGT TCCTGCTTTC TTCTCCGAGG ACGACTCCCA GTCGAATGAC
    TCCAGCGACT CGGACAGCAG CAGCAGCCAG AGTGACGATG TGGATCAGGA GACGTTTCTG
    CTGGACGAGC CTCTTGAGAG AACCACCGGC TCAGCACATG CTAACAGTGC AGCCCAGGCC
    CCACGCTCCA TGCAGTGGGC TGTTCGCACT ACACCCAGCC AGCGCTCTGG AGGTGGCGCC
    CCATCCAGCT CGTCCGCTCC TGCTGCGAGC TCCACTGGCC TCATCTACAT CGACCCGTCA
    AACCTGCGCC GCAGCAGCGC CATCAGCACC AGCGCAGCCG CTGCCGCAGC AGCCCTGGAG
    GCCAGTAACT CCAGCAGTTA TCTGACGTCT GCCAGCAGCC TGGCTCGAGC ATACAGCATC
    GTCATCCGCC AGATCTCTGA CCTCATGAGT CTCATTCCCA AATACAACCA CCTGGTCTAC
    TCGCAGTACC CCGCTGCTGT CAAACTCACC TATCAGGATG CTGTCAATCT GCAGAACTTT
    GTGGAGGACA AGTTGATTCC CACCTGGAAC TGGATGGTGT CCATCATGGA CTCCACTGAA
    GCGCAGCTGC GTTACGGCTC GGCTCTGTCT TCAGCCGGGG ATCCTGGTCA TCCCAGCCAC
    CCGCTACACG CATCCCAGCA TGCCGGCCGC AGAGAGCGCA TGACCGCTCG AGAGGAGGCC
    AGTCTGCGCA CTCTAGAAGG ACGAAGGCGT GCTGCTACGC TTCTGACGGC ACGTCAGGGC
    ATGATGTCAG CGCGAGGAGA TTTCCTGAAC TACGCTCTGT CTCTGATGCG TTCCCATAAT
    GACGAGCATT CAGACGTTCT GCCTGTGTTA GATGTCTGTT CGCTCAAACA CGTGGCTTAC
    GTCTTCCAGG CGCTCATCTA CTGGATTAAA GCCATGAATC TGCAGACCAC GCTCGACACC
    ACTCAGATTG ACAGGAAGAG GAATCGTGAG CTGCTGGAGC TCGGTCTGGA CAATGAAGAC
    TCTGAGCACG AGAACGATGA GGACACCAAT CAGAGCTCTA CACTGCAGGA TAAGGACGAG
    GAGCCTGTGC CTGCAGAGAC TGGACAACAC CATCCGTTTT TCCGCCGCTC GGACTCCATG
    ACCTTCCTGG GGTGCATCCC TCCAAACCCT TTTGAAGTTC CTCTAGCAGA GGCCATTCCT
    CTGGCTGACC AGCCGCATCT CCTGCAGCCC AATGCCAGAA AGGAGGATTT GTTTGGACGG
    CCTAGTCAAG GTCTATATTC GTCCTCATAT ATGGCCAGTA AAGGCCTCAC CGATCTGACT
    GTGGACATGA ATTGCTTGCA GATGTCGTAT TCGGCGAACA TGAAGAACGT GATGAGTATG
    GAGTCCCGGC AGAGGGGTGG CGAGGAGCAG CCGGTGGCGG AGCAGGAGAT GGATGTGTCT
    AAACCCGGCC CTTCACCACA CGATCTGGCG GCCCAGTTAA AGAGCAGCCT GCTGGCAGAG
    ATTGGCCTGA CTGAGAGTGA TGGCCCACCG CTGCCCACGT TTATTCCACA CTGTAGTTTT
    ATGGGGATGG TGATCTCTCA TGACATGCTG CTGGGCCGCT GGCGGCTTTC TCTGGAGCTG
    TTTGGCCGTG TTTTCATGGA GGACGTTGGA GCAGAACCAG GATCTATCCT GACAGAGCTG
    GGGGGTTTTG AGGTAAAGGA GTCCAAGTTT CGTCGTGAGA TGGAGAAGCT GCGTAACCTT
    CAGTCGCGTG ATCTGGCCCT GGAGGTGGAC CGAGACCGTG AGCAGCTCAT CCAGCAGACC
    ATGCGGCAGC TGAACGCTCA CTTCGGCCGC CGCTGTACCA CCACACCCAT GGCCGTGCAC
    CGCGTAAAGG TGACATTCAA GGATGAGCCT GGAGAGGGCA GTGGTGTCGC TCGCAGCTTC
    TACACCGCCA TCGCTCAGGC CTTCTTGTCC AACGACAAGC TGCCCAATCT GGACTGCGTG
    CAGAGCGTCA GCAAGGGGAT GCAGGCTAGC AATCTTATGC AGCGGCTGAG GAACCGAGAT
    CGTGAGCGTG AGAGGAGGAG CGGAGGACTG AGAGCTGCAT CCAGACGAGA TCGTGACAGG
    GACTCGCGGA GGCAGTTGTC CATCGACACC CGGCCGTTCA GACCTGCATC AGAAGGAAAC
    CCCAGTGATG AACCAGAACC GCTGCCGGCT CACAGACAGG CTCTGGGAGA GAGACTTTAC
    CCACGAGTTC ATGCCATGCA ACCGGCGTTT GCTAGTAAAA TCACAGGGAT GCTGCTGGAA
    CTGTCTCCCG CTCAGCTGCT GCTACTCCTG GCCAGCGAAG ACTCGCTCAG AGCCCGAGTG
    GAGGAGGCCA TGGAGCTGCT CATAACTCAT GGACGGGAAA ATGGTGCTGA CAGTATACTA
    GACCTCGGCC TCCTCGACAC TCCTGAGAAA GCACAACAGG AGAACCGAAA GAGGCATGGT
    TCGACTCGCA GTGTGGTGGA CATGGAGCTG GATGACCCTG ATGATGGGGA TGATAACGCT
    CCTCTGTTTT ACCAGCCTGG AAAGAGGGGT TTCTACTCAC CACGTCCCGG CAAGAACACT
    GAGGCCAGGC TCAACTGTTT CCGTAACATC GGCAGGATAC TGGGTTTGTG TCTGCTACAG
    AATGAGCTTT GCCCAATCAC TCTCAACAGA CACGTCATCA AGGTGCTGCT CGGCAGAAAG
    GTGAACTGGC ACGACTTTGC GTTCTTCGAC CCGGTAATGT ACGAGAGTTT GCGGCAGCTC
    ATCCGTCACT CTCAGACTGA AGAGGCGGAG GCTGTGTTTG CGGCGATGGA CCTGGCCTTC
    GCTATCGACC TCTGCAAGGA GGAGGGAGCA GGACAGGTGG AGCTGCTGTC CGGTGGAGTC
    AACATGCCGG TGACGCCTCT GAATGTGTAC GAGTACGTGA GGAGATACGC AGAGCACCGG
    ATGCTGGTGG TGGCAGAACA GCCTCTGCAT GCCATGCGGA AAGGTCTTCT GGATGTGCTT
    CCTAAGAACG CTCTGGAAGA TCTGACAGCG GAGGATTTCA GACTGCTGGT CAACGGCTGT
    GGAGAAGTCA ACGTCCAGAT GCTCATTAGT TTCACTTCAT TCAATGATGA GTCAGGTGAG
    AATGCAGAGA AGCTGCTGCA GTTCAAGCGC TGGTTTTGGT CCATCGTTGA GAAGATGAGC
    ATGACGGAAA GGCAGGACCT GGTGTACTTC TGGACGTCCA GCCCGTCTCT GCCGGCCAGT
    GAAGAAGGTT TCCAGCCGAT GCCCTCCATC ACCATCCGCC CGCCGGACGA CCAGCACCTG
    CCCACAGCCA ACACCTGCAT CTCTCGCCTC TACGTGCCAC TTTACTCTTC CAAACAGATC
    CTCAAACAGA AACTTTTACT AGCCATCAAG ACCAAGAACT TCGGTTTTGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22032
    Clone ID Related Accession (Same CDS sequence) XM_005158440.3 , XM_005158440.4
    Accession Version XM_005158440.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase UBR5 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158440.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGAGA GAGACTCTGA GCTGCTCCGC GAGCGTGAGT CGGTCCTGAG GCTGCGGGAG
    AGGCGGTGGC TGGACGGGGC CTCCTTCGAT GCGGAGCGTG GCTCCACCAG CCGCGAGGGA
    GAGCCCAGCC TTGACAAGAA GAGTGTCCCG CTGCAGAGCC CAGTCACTCT GGGGGAAGAG
    CTCCAGTGGT GGCCTGATAA GGACTATGTA ACAAAGTTTG TCAGCATTGG TGCTTTGTAC
    TCGGAGCTGG TTGCCGTCAG TACCAAAGGA GAACTCTACC AGTGGAAGTG GAATGAACCT
    GAACCTTACA GAAACGCACA AAATCCTTCT ATACATCACC CTCGTGTTCC TTTCCTGGGC
    TTGACCAATG AAAAGATCAC CCACCTGTCC GCCAACAGTA TCCGAGCCAC TGTGGCCACC
    GAGAACAACA AGGTGGCAAC ATGGGTGGAT GAAACTCTGA GCACTGTTGC TGCTAAACTC
    GAACACGGAG CACAAACGTT CCCGGAGCTG CAGGGGGAGC GGATCGTCTC TCTGCACTGC
    TGTGCCCTGT ATACCTGCGC TCAGCTGGAG AACAGCCTTT ACTGGTGGGG TGTTGTGCCT
    TTTAGTCAAC GGAAAAAAAT GCTTGAAAAG GCCAGAGCCA AGAACAAGAA ACCAAAGTCC
    AGTGCTGGAA TCTCTTCGAT ACCCAACATC ACAGTAGGAA CTCAGGTGTG CCTGAGGAAT
    AATCCTCTTT ATCACGCTGG TGCCGTGGCC TTTTCCGTGA ACGCCGGGAT CCCTAAAGTG
    GGCGTCCTGC TGGAGTCGGT GTGGAACATG AACGACAGCT GCCGCTTCCA GCTGCGCTCG
    CCAGAGAGCC TGAAAAACAT GGAGAAAAAC ACCAAGACAC AGGAGACCAA GACGGAGAGC
    AAGCCAGAGC TGGTGAAGAC GGAAATGGGC CCTCCGCCCT CACCAGCGTC CACATGCAGT
    GACACTTCAT CCATCGCTAG CAGCGCTTCA CTGCCGTACA AGCGAAGGCG CTCAACACCG
    GCTCCGAAGG AGGAGGAGAA GGTGAATGAG GAGCAGTGGC CTCTGCGAGA GGTTGTTTTC
    GTGGAGGATG TTAAAAACGT CCCCGTGGGA AAGGTGCTAA AAGTCGATGG TGCATATGTT
    GCTGTGAAAT TTCCAGGGAC GTCGAGCAGC GTGAGCAGCC AGAGCGCAGC TCCCACTGAC
    TCTGACCCGT CCTCACTGCT GCAAGACTGT CGACTGCTCA GAATAGATGA GCTACAGGTT
    GTTAAAACTG GTGGAACACC AAAAGTTCCT GACTGTTTTC AGCGAACACC TAAAAAACTT
    TGCATCCCAG AAAAAGCTGA AATTTTAGCA GTGAATGTTG ACTCCAAAGG AGTTCATGCT
    GTGTTGAAAA CGGGCAGCTG GGTGAGATAC TGTGTCTTTG ACTTAGCCAC CGGCAAAGCC
    GAGCAAGAGA ATCACTTTCC CACCAGTAAC CTGGCGTTTC TTGGTCAGAG CGAGCGAAAT
    GTGGCCATCT TCACTGCTGG CCAGGAGAGT CCCATTATTT TGAGGGATGG CAATGGCACT
    ATTTATCCCA TGGCTAAAGA CTGTATGGGT GGAATACGTG ACCCTGATTG GCTGGATCTG
    CCGCCTATAG CCAGTCTGGG CATGGGAGTT CACTCGCTCG CCAACCTTCC CAGTAACTCC
    ACAATCAAAA AGAAGGCGGC CATCATCATA ATGGCTGTAG AGAAGCAGAC TCTGATGCAG
    CACGTGCTGC GCTGTGATTA CGAGGCGTGT AGGCAGTATC TGATCAGTCT GGAGCAGGCC
    ATGCTACTGG AGCAGAACCC TCACGCTCTG GACACTCTGC TAGGACACCG CTGTGACGGA
    AACCGCAACG TCCTCCATGC CTGTGTGTCC GTCTGCTTCC CCGTCAGCAA CAAGGAAACC
    AAGGAGGAAG AAGAAGCAGA GCGGTCTGAA AGAAACACGT TCGCTGAGAG GCTTTCTGCA
    GTGGAGGCCA TTGCCAATGC CATATCAGTG GTGTCGAGCA ACAGCTCCGG GAACCGGACA
    GGATCATCCA GCAGCAGGGG CTTGCGTTTG AGAGAGATGA TGCGGCGCTC ACTCCGTGCT
    GCAGGTTTAG GCCGACATGA ATCCGGACCT TCCTCCAGCG ACCATCAAGA CCCCGTTTCA
    CCCCCGATAG CACCTCCTAG CTGGGTACCA GACCCTCCAC CGATGGATCC TGACGGTGAC
    ATTGATTTCA TCTTGGCCCC TGCAGTGGGC TCCCTCACCA CTGCTTCCAC AGGCACCAGC
    CAGGGACCCA GCACTTCCAC CATCCCAGGA CCCTCCTCTG AACCATCAGT AGTGGAATCT
    AAAGACCGTA AGGCGAACGC ACACCTGATA CTGAAGCTGA TGTGTGACAG CATCGTCCTG
    CGGCCTCATC TAAGAGAACT GCTCTCTGCC AAGGATGCTC GTGGAATGAC TCCATTCATG
    TTAGCAGTGA GTGGCAGAGC TTACCCAGCA GCCATTACTG TCTTAGAAGC AGCACAGAAA
    ATTGCCAAAG GTGAACCAGG TCTTGGAGAG AAAGAGGATA CAGCATCTGT GTTCATGGAG
    ATGATCTGTC CTTCAGGAAC CAATCCAGAC GACTCTCCGC TGTACGTCCT GTGCTGTAAC
    GACACCTGCA GCTTCACCTG GACTGGAGCA GAACACATCA ACCAGGACAT CTTTGAGTGC
    AGAACTTGTG GCCTGCTGGA GTCGCTGTGC TGCTGCACTG AATGTGCTAG AGTCTGTCAC
    AAGGGGCACG ACTGCAAACT GAAGAGGACG TCACCAACAG CTTACTGTGA CTGCTGGGAG
    AAGTGCAAAT GCAAGACTCT CATCGCAGGC CAGAAAGCGG CACGTCTGGA TCTGCTCTAC
    AGGCTGCTGA CCACCACTAA TCTAGTGACC ACTCCTAACA GCAGGGGAGA GCACATCTTG
    CTGTTTCTGG TGCAGACTGT TGCAAGGCAG AGTGTCGAAC ACTGTCAGTA TCGACCTCCT
    CGCATTCGAG AGGACAGGAA CCGGAAAGCT GCCAGTGCTG AAGACTCTGA TATGCCAGAT
    CATGACCTGG AACCTCCACG CTTTGCTCAG CTGGCTCTGG AGCGAGTGTT GCAGGACTGG
    AACGCACTGA AGTCAATGAT AATGTTCGGC TCTCAGGAAA ATAAAGATCC GTTAAGCGCT
    AGCAGCAGAA TCGCTCACCT TTTACCGGAG GAGCAGATGT ACCTCAACCA GCAGAGTGGC
    ACCATCAGAC TGGACTGTTT CACACACTGC CTTATTGTCA AGTGTGCACC AGACATCACT
    TTCATTGACA CACTGTTAGG GACCCTGGTG AAGGAGCTGC AGAATAAATA CACCCCCGGC
    CGGCGGGAAG AGGCCATCAA TGTCACACGG AGGTTCTTGC GCTCTGTGGC CAGAGTGTTT
    GTCATCCTCA GCGTGGAGAT GGCATCTTCC AAGAAAAAGA ACAACTTCAT CCCTCAGCCT
    ATTGGGAAGT GCAGGCGTGT GTTCCAGGCA CTGTTGCCGT ACGCAGTGGA GGAGCTCTGC
    AATGTGGCCG AGTCTCTGAT CGTGCCGGTG CGTATGGGCA TCGCTCGGCC CACAGCTCCC
    TTCACTCTGG CCAGTACCAG CATTGATGCA GTGCAGGGCA GTGAGGAGCT CTTCTCTGTG
    GAGCCTCTGC CACCTCGACC CTCCCCGGAC CAGTCCAGCA GCTCCAGTCA GTCTGCCTCT
    TCATACATCA TCAGGAACCC GCAGCCCCGC CGCAGCAGCC AGTCTCAGAC AGCCCGCGGA
    CGGGACGAGG AGCAGGATGA TATCGTGTCT GCTGACGTAG AAGAGGTGGA GGTGGTGGAG
    GGTGTTGCAG GTGAGGAGGA TCACCATGAT GACCAGGAGG AGCAGGGAGA GGAGAACGCA
    GAGGCTGAAG GACAGCATGA TGAGCACGAT GAGGATGGGA GCGACATGGA GCTGGATTTA
    TTGGCTGCCG CTGAGACCGA GAGTGACAGT GAGAGTAACC ATAGCAACCA GGACAATGCG
    AGTGGCCGCA GGAGTGTTGT GACGGCAGCC ACTGCTGGAT CTGAAGCAGG TGCCAGCAGT
    GTTCCTGCTT TCTTCTCCGA GGACGACTCC CAGTCGAATG ACTCCAGCGA CTCGGACAGC
    AGCAGCAGCC AGAGTGACGA TGTGGATCAG GAGACGTTTC TGCTGGACGA GCCTCTTGAG
    AGAACCACCG GCTCAGCACA TGCTAACAGT GCAGCCCAGG CCCCACGCTC CATGCAGTGG
    GCTGTTCGCA CTACACCCAG CCAGCGCTCT GGAGGTGGCG CCCCATCCAG CTCGTCCGCT
    CCTGCTGCGA GCTCCACTGG CCTCATCTAC ATCGACCCGT CAAACCTGCG CCGCAGCAGC
    GCCATCAGCA CCAGCGCAGC CGCTGCCGCA GCAGCCCTGG AGGCCAGTAA CTCCAGCAGT
    TATCTGACGT CTGCCAGCAG CCTGGCTCGA GCATACAGCA TCGTCATCCG CCAGATCTCT
    GACCTCATGA GTCTCATTCC CAAATACAAC CACCTGGTCT ACTCGCAGTA CCCCGCTGCT
    GTCAAACTCA CCTATCAGGA TGCTGTCAAT CTGCAGAACT TTGTGGAGGA CAAGTTGATT
    CCCACCTGGA ACTGGATGGT GTCCATCATG GACTCCACTG AAGCGCAGCT GCGTTACGGC
    TCGGCTCTGT CTTCAGCCGG GGATCCTGGT CATCCCAGCC ACCCGCTACA CGCATCCCAG
    CATGCCGGCC GCAGAGAGCG CATGACCGCT CGAGAGGAGG CCAGTCTGCG CACTCTAGAA
    GGACGAAGGC GTGCTGCTAC GCTTCTGACG GCACGTCAGG GCATGATGTC AGCGCGAGGA
    GATTTCCTGA ACTACGCTCT GTCTCTGATG CGTTCCCATA ATGACGAGCA TTCAGACGTT
    CTGCCTGTGT TAGATGTCTG TTCGCTCAAA CACGTGGCTT ACGTCTTCCA GGCGCTCATC
    TACTGGATTA AAGCCATGAA TCTGCAGACC ACGCTCGACA CCACTCAGAT TGACAGGAAG
    AGGAATCGTG AGCTGCTGGA GCTCGGTCTG GACAATGAAG ACTCTGAGCA CGAGAACGAT
    GAGGACACCA ATCAGAGCTC TACACTGCAG GATAAGGACG AGGAGCCTGT GCCTGCAGAG
    ACTGGACAAC ACCATCCGTT TTTCCGCCGC TCGGACTCCA TGACCTTCCT GGGGTGCATC
    CCTCCAAACC CTTTTGAAGT TCCTCTAGCA GAGGCCATTC CTCTGGCTGA CCAGCCGCAT
    CTCCTGCAGC CCAATGCCAG AAAGGAGGAT TTGTTTGGAC GGCCTAGTCA AGGTCTATAT
    TCGTCCTCAT ATATGGCCAG TAAAGGCCTC ACCGATCTGA CTGTGGACAT GAATTGCTTG
    CAGATTCTAC CCACAAAGAT GTCGTATTCG GCGAACATGA AGAACGTGAT GAGTATGGAG
    TCCCGGCAGA GGGGTGGCGA GGAGCAGCCG GTGGCGGAGC AGGAGATGGA TGTGTCTAAA
    CCCGGCCCTT CACCACACGA TCTGGCGGCC CAGTTAAAGA GCAGCCTGCT GGCAGAGATT
    GGCCTGACTG AGAGTGATGG CCCACCGCTG CCCACGTTTA TTCCACACTG TAGTTTTATG
    GGGATGGTGA TCTCTCATGA CATGCTGCTG GGCCGCTGGC GGCTTTCTCT GGAGCTGTTT
    GGCCGTGTTT TCATGGAGGA CGTTGGAGCA GAACCAGGAT CTATCCTGAC AGAGCTGGGG
    GGTTTTGAGG TAAAGGAGTC CAAGTTTCGT CGTGAGATGG AGAAGCTGCG TAACCTTCAG
    TCGCGTGATC TGGCCCTGGA GGTGGACCGA GACCGTGAGC AGCTCATCCA GCAGACCATG
    CGGCAGCTGA ACGCTCACTT CGGCCGCCGC TGTACCACCA CACCCATGGC CGTGCACCGC
    GTAAAGGTGA CATTCAAGGA TGAGCCTGGA GAGGGCAGTG GTGTCGCTCG CAGCTTCTAC
    ACCGCCATCG CTCAGGCCTT CTTGTCCAAC GACAAGCTGC CCAATCTGGA CTGCGTGCAG
    AGCGTCAGCA AGGGGATGCA GGCTAGCAAT CTTATGCAGC GGCTGAGGAA CCGAGATCGT
    GAGCGTGAGA GGAGGAGCGG AGGACTGAGA GCTGCATCCA GACGAGATCG TGACAGGGAC
    TCGCGGAGGC AGTTGTCCAT CGACACCCGG CCGTTCAGAC CTGCATCAGA AGGAAACCCC
    AGTGATGAAC CAGAACCGCT GCCGGCTCAC AGACAGGCTC TGGGAGAGAG ACTTTACCCA
    CGAGTTCATG CCATGCAACC GGCGTTTGCT AGTAAAATCA CAGGGATGCT GCTGGAACTG
    TCTCCCGCTC AGCTGCTGCT ACTCCTGGCC AGCGAAGACT CGCTCAGAGC CCGAGTGGAG
    GAGGCCATGG AGCTGCTCAT AACTCATGGA CGGGAAAATG GTGCTGACAG TATACTAGAC
    CTCGGCCTCC TCGACACTCC TGAGAAAGCA CAACAGGAGA ACCGAAAGAG GCATGGTTCG
    ACTCGCAGTG TGGTGGACAT GGAGCTGGAT GACCCTGATG ATGGGGATGA TAACGCTCCT
    CTGTTTTACC AGCCTGGAAA GAGGGGTTTC TACTCACCAC GTCCCGGCAA GAACACTGAG
    GCCAGGCTCA ACTGTTTCCG TAACATCGGC AGGATACTGG GTTTGTGTCT GCTACAGAAT
    GAGCTTTGCC CAATCACTCT CAACAGACAC GTCATCAAGG TGCTGCTCGG CAGAAAGGTG
    AACTGGCACG ACTTTGCGTT CTTCGACCCG GTAATGTACG AGAGTTTGCG GCAGCTCATC
    CGTCACTCTC AGACTGAAGA GGCGGAGGCT GTGTTTGCGG CGATGGACCT GGCCTTCGCT
    ATCGACCTCT GCAAGGAGGA GGGAGCAGGA CAGGTGGAGC TGCTGTCCGG TGGAGTCAAC
    ATGCCGGTGA CGCCTCTGAA TGTGTACGAG TACGTGAGGA GATACGCAGA GCACCGGATG
    CTGGTGGTGG CAGAACAGCC TCTGCATGCC ATGCGGAAAG GTCTTCTGGA TGTGCTTCCT
    AAGAACGCTC TGGAAGATCT GACAGCGGAG GATTTCAGAC TGCTGGTCAA CGGCTGTGGA
    GAAGTCAACG TCCAGATGCT CATTAGTTTC ACTTCATTCA ATGATGAGTC AGGTGAGAAT
    GCAGAGAAGC TGCTGCAGTT CAAGCGCTGG TTTTGGTCCA TCGTTGAGAA GATGAGCATG
    ACGGAAAGGC AGGACCTGGT GTACTTCTGG ACGTCCAGCC CGTCTCTGCC GGCCAGTGAA
    GAAGGTTTCC AGCCGATGCC CTCCATCACC ATCCGCCCGC CGGACGACCA GCACCTGCCC
    ACAGCCAACA CCTGCATCTC TCGCCTCTAC GTGCCACTTT ACTCTTCCAA ACAGATCCTC
    AAACAGAAAC TTTTACTAGC CATCAAGACC AAGAACTTCG GTTTTGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158497.1
    CDS234..8684
    Translation

    Target ORF information:

    RefSeq Version XM_005158440.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158440.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGAGA GAGACTCTGA GCTGCTCCGC GAGCGTGAGT CGGTCCTGAG GCTGCGGGAG
    AGGCGGTGGC TGGACGGGGC CTCCTTCGAT GCGGAGCGTG GCTCCACCAG CCGCGAGGGA
    GAGCCCAGCC TTGACAAGAA GAGTGTCCCG CTGCAGAGCC CAGTCACTCT GGGGGAAGAG
    CTCCAGTGGT GGCCTGATAA GGACTATGTA ACAAAGTTTG TCAGCATTGG TGCTTTGTAC
    TCGGAGCTGG TTGCCGTCAG TACCAAAGGA GAACTCTACC AGTGGAAGTG GAATGAACCT
    GAACCTTACA GAAACGCACA AAATCCTTCT ATACATCACC CTCGTGTTCC TTTCCTGGGC
    TTGACCAATG AAAAGATCAC CCACCTGTCC GCCAACAGTA TCCGAGCCAC TGTGGCCACC
    GAGAACAACA AGGTGGCAAC ATGGGTGGAT GAAACTCTGA GCACTGTTGC TGCTAAACTC
    GAACACGGAG CACAAACGTT CCCGGAGCTG CAGGGGGAGC GGATCGTCTC TCTGCACTGC
    TGTGCCCTGT ATACCTGCGC TCAGCTGGAG AACAGCCTTT ACTGGTGGGG TGTTGTGCCT
    TTTAGTCAAC GGAAAAAAAT GCTTGAAAAG GCCAGAGCCA AGAACAAGAA ACCAAAGTCC
    AGTGCTGGAA TCTCTTCGAT ACCCAACATC ACAGTAGGAA CTCAGGTGTG CCTGAGGAAT
    AATCCTCTTT ATCACGCTGG TGCCGTGGCC TTTTCCGTGA ACGCCGGGAT CCCTAAAGTG
    GGCGTCCTGC TGGAGTCGGT GTGGAACATG AACGACAGCT GCCGCTTCCA GCTGCGCTCG
    CCAGAGAGCC TGAAAAACAT GGAGAAAAAC ACCAAGACAC AGGAGACCAA GACGGAGAGC
    AAGCCAGAGC TGGTGAAGAC GGAAATGGGC CCTCCGCCCT CACCAGCGTC CACATGCAGT
    GACACTTCAT CCATCGCTAG CAGCGCTTCA CTGCCGTACA AGCGAAGGCG CTCAACACCG
    GCTCCGAAGG AGGAGGAGAA GGTGAATGAG GAGCAGTGGC CTCTGCGAGA GGTTGTTTTC
    GTGGAGGATG TTAAAAACGT CCCCGTGGGA AAGGTGCTAA AAGTCGATGG TGCATATGTT
    GCTGTGAAAT TTCCAGGGAC GTCGAGCAGC GTGAGCAGCC AGAGCGCAGC TCCCACTGAC
    TCTGACCCGT CCTCACTGCT GCAAGACTGT CGACTGCTCA GAATAGATGA GCTACAGGTT
    GTTAAAACTG GTGGAACACC AAAAGTTCCT GACTGTTTTC AGCGAACACC TAAAAAACTT
    TGCATCCCAG AAAAAGCTGA AATTTTAGCA GTGAATGTTG ACTCCAAAGG AGTTCATGCT
    GTGTTGAAAA CGGGCAGCTG GGTGAGATAC TGTGTCTTTG ACTTAGCCAC CGGCAAAGCC
    GAGCAAGAGA ATCACTTTCC CACCAGTAAC CTGGCGTTTC TTGGTCAGAG CGAGCGAAAT
    GTGGCCATCT TCACTGCTGG CCAGGAGAGT CCCATTATTT TGAGGGATGG CAATGGCACT
    ATTTATCCCA TGGCTAAAGA CTGTATGGGT GGAATACGTG ACCCTGATTG GCTGGATCTG
    CCGCCTATAG CCAGTCTGGG CATGGGAGTT CACTCGCTCG CCAACCTTCC CAGTAACTCC
    ACAATCAAAA AGAAGGCGGC CATCATCATA ATGGCTGTAG AGAAGCAGAC TCTGATGCAG
    CACGTGCTGC GCTGTGATTA CGAGGCGTGT AGGCAGTATC TGATCAGTCT GGAGCAGGCC
    ATGCTACTGG AGCAGAACCC TCACGCTCTG GACACTCTGC TAGGACACCG CTGTGACGGA
    AACCGCAACG TCCTCCATGC CTGTGTGTCC GTCTGCTTCC CCGTCAGCAA CAAGGAAACC
    AAGGAGGAAG AAGAAGCAGA GCGGTCTGAA AGAAACACGT TCGCTGAGAG GCTTTCTGCA
    GTGGAGGCCA TTGCCAATGC CATATCAGTG GTGTCGAGCA ACAGCTCCGG GAACCGGACA
    GGATCATCCA GCAGCAGGGG CTTGCGTTTG AGAGAGATGA TGCGGCGCTC ACTCCGTGCT
    GCAGGTTTAG GCCGACATGA ATCCGGACCT TCCTCCAGCG ACCATCAAGA CCCCGTTTCA
    CCCCCGATAG CACCTCCTAG CTGGGTACCA GACCCTCCAC CGATGGATCC TGACGGTGAC
    ATTGATTTCA TCTTGGCCCC TGCAGTGGGC TCCCTCACCA CTGCTTCCAC AGGCACCAGC
    CAGGGACCCA GCACTTCCAC CATCCCAGGA CCCTCCTCTG AACCATCAGT AGTGGAATCT
    AAAGACCGTA AGGCGAACGC ACACCTGATA CTGAAGCTGA TGTGTGACAG CATCGTCCTG
    CGGCCTCATC TAAGAGAACT GCTCTCTGCC AAGGATGCTC GTGGAATGAC TCCATTCATG
    TTAGCAGTGA GTGGCAGAGC TTACCCAGCA GCCATTACTG TCTTAGAAGC AGCACAGAAA
    ATTGCCAAAG GTGAACCAGG TCTTGGAGAG AAAGAGGATA CAGCATCTGT GTTCATGGAG
    ATGATCTGTC CTTCAGGAAC CAATCCAGAC GACTCTCCGC TGTACGTCCT GTGCTGTAAC
    GACACCTGCA GCTTCACCTG GACTGGAGCA GAACACATCA ACCAGGACAT CTTTGAGTGC
    AGAACTTGTG GCCTGCTGGA GTCGCTGTGC TGCTGCACTG AATGTGCTAG AGTCTGTCAC
    AAGGGGCACG ACTGCAAACT GAAGAGGACG TCACCAACAG CTTACTGTGA CTGCTGGGAG
    AAGTGCAAAT GCAAGACTCT CATCGCAGGC CAGAAAGCGG CACGTCTGGA TCTGCTCTAC
    AGGCTGCTGA CCACCACTAA TCTAGTGACC ACTCCTAACA GCAGGGGAGA GCACATCTTG
    CTGTTTCTGG TGCAGACTGT TGCAAGGCAG AGTGTCGAAC ACTGTCAGTA TCGACCTCCT
    CGCATTCGAG AGGACAGGAA CCGGAAAGCT GCCAGTGCTG AAGACTCTGA TATGCCAGAT
    CATGACCTGG AACCTCCACG CTTTGCTCAG CTGGCTCTGG AGCGAGTGTT GCAGGACTGG
    AACGCACTGA AGTCAATGAT AATGTTCGGC TCTCAGGAAA ATAAAGATCC GTTAAGCGCT
    AGCAGCAGAA TCGCTCACCT TTTACCGGAG GAGCAGATGT ACCTCAACCA GCAGAGTGGC
    ACCATCAGAC TGGACTGTTT CACACACTGC CTTATTGTCA AGTGTGCACC AGACATCACT
    TTCATTGACA CACTGTTAGG GACCCTGGTG AAGGAGCTGC AGAATAAATA CACCCCCGGC
    CGGCGGGAAG AGGCCATCAA TGTCACACGG AGGTTCTTGC GCTCTGTGGC CAGAGTGTTT
    GTCATCCTCA GCGTGGAGAT GGCATCTTCC AAGAAAAAGA ACAACTTCAT CCCTCAGCCT
    ATTGGGAAGT GCAGGCGTGT GTTCCAGGCA CTGTTGCCGT ACGCAGTGGA GGAGCTCTGC
    AATGTGGCCG AGTCTCTGAT CGTGCCGGTG CGTATGGGCA TCGCTCGGCC CACAGCTCCC
    TTCACTCTGG CCAGTACCAG CATTGATGCA GTGCAGGGCA GTGAGGAGCT CTTCTCTGTG
    GAGCCTCTGC CACCTCGACC CTCCCCGGAC CAGTCCAGCA GCTCCAGTCA GTCTGCCTCT
    TCATACATCA TCAGGAACCC GCAGCCCCGC CGCAGCAGCC AGTCTCAGAC AGCCCGCGGA
    CGGGACGAGG AGCAGGATGA TATCGTGTCT GCTGACGTAG AAGAGGTGGA GGTGGTGGAG
    GGTGTTGCAG GTGAGGAGGA TCACCATGAT GACCAGGAGG AGCAGGGAGA GGAGAACGCA
    GAGGCTGAAG GACAGCATGA TGAGCACGAT GAGGATGGGA GCGACATGGA GCTGGATTTA
    TTGGCTGCCG CTGAGACCGA GAGTGACAGT GAGAGTAACC ATAGCAACCA GGACAATGCG
    AGTGGCCGCA GGAGTGTTGT GACGGCAGCC ACTGCTGGAT CTGAAGCAGG TGCCAGCAGT
    GTTCCTGCTT TCTTCTCCGA GGACGACTCC CAGTCGAATG ACTCCAGCGA CTCGGACAGC
    AGCAGCAGCC AGAGTGACGA TGTGGATCAG GAGACGTTTC TGCTGGACGA GCCTCTTGAG
    AGAACCACCG GCTCAGCACA TGCTAACAGT GCAGCCCAGG CCCCACGCTC CATGCAGTGG
    GCTGTTCGCA CTACACCCAG CCAGCGCTCT GGAGGTGGCG CCCCATCCAG CTCGTCCGCT
    CCTGCTGCGA GCTCCACTGG CCTCATCTAC ATCGACCCGT CAAACCTGCG CCGCAGCAGC
    GCCATCAGCA CCAGCGCAGC CGCTGCCGCA GCAGCCCTGG AGGCCAGTAA CTCCAGCAGT
    TATCTGACGT CTGCCAGCAG CCTGGCTCGA GCATACAGCA TCGTCATCCG CCAGATCTCT
    GACCTCATGA GTCTCATTCC CAAATACAAC CACCTGGTCT ACTCGCAGTA CCCCGCTGCT
    GTCAAACTCA CCTATCAGGA TGCTGTCAAT CTGCAGAACT TTGTGGAGGA CAAGTTGATT
    CCCACCTGGA ACTGGATGGT GTCCATCATG GACTCCACTG AAGCGCAGCT GCGTTACGGC
    TCGGCTCTGT CTTCAGCCGG GGATCCTGGT CATCCCAGCC ACCCGCTACA CGCATCCCAG
    CATGCCGGCC GCAGAGAGCG CATGACCGCT CGAGAGGAGG CCAGTCTGCG CACTCTAGAA
    GGACGAAGGC GTGCTGCTAC GCTTCTGACG GCACGTCAGG GCATGATGTC AGCGCGAGGA
    GATTTCCTGA ACTACGCTCT GTCTCTGATG CGTTCCCATA ATGACGAGCA TTCAGACGTT
    CTGCCTGTGT TAGATGTCTG TTCGCTCAAA CACGTGGCTT ACGTCTTCCA GGCGCTCATC
    TACTGGATTA AAGCCATGAA TCTGCAGACC ACGCTCGACA CCACTCAGAT TGACAGGAAG
    AGGAATCGTG AGCTGCTGGA GCTCGGTCTG GACAATGAAG ACTCTGAGCA CGAGAACGAT
    GAGGACACCA ATCAGAGCTC TACACTGCAG GATAAGGACG AGGAGCCTGT GCCTGCAGAG
    ACTGGACAAC ACCATCCGTT TTTCCGCCGC TCGGACTCCA TGACCTTCCT GGGGTGCATC
    CCTCCAAACC CTTTTGAAGT TCCTCTAGCA GAGGCCATTC CTCTGGCTGA CCAGCCGCAT
    CTCCTGCAGC CCAATGCCAG AAAGGAGGAT TTGTTTGGAC GGCCTAGTCA AGGTCTATAT
    TCGTCCTCAT ATATGGCCAG TAAAGGCCTC ACCGATCTGA CTGTGGACAT GAATTGCTTG
    CAGATTCTAC CCACAAAGAT GTCGTATTCG GCGAACATGA AGAACGTGAT GAGTATGGAG
    TCCCGGCAGA GGGGTGGCGA GGAGCAGCCG GTGGCGGAGC AGGAGATGGA TGTGTCTAAA
    CCCGGCCCTT CACCACACGA TCTGGCGGCC CAGTTAAAGA GCAGCCTGCT GGCAGAGATT
    GGCCTGACTG AGAGTGATGG CCCACCGCTG CCCACGTTTA TTCCACACTG TAGTTTTATG
    GGGATGGTGA TCTCTCATGA CATGCTGCTG GGCCGCTGGC GGCTTTCTCT GGAGCTGTTT
    GGCCGTGTTT TCATGGAGGA CGTTGGAGCA GAACCAGGAT CTATCCTGAC AGAGCTGGGG
    GGTTTTGAGG TAAAGGAGTC CAAGTTTCGT CGTGAGATGG AGAAGCTGCG TAACCTTCAG
    TCGCGTGATC TGGCCCTGGA GGTGGACCGA GACCGTGAGC AGCTCATCCA GCAGACCATG
    CGGCAGCTGA ACGCTCACTT CGGCCGCCGC TGTACCACCA CACCCATGGC CGTGCACCGC
    GTAAAGGTGA CATTCAAGGA TGAGCCTGGA GAGGGCAGTG GTGTCGCTCG CAGCTTCTAC
    ACCGCCATCG CTCAGGCCTT CTTGTCCAAC GACAAGCTGC CCAATCTGGA CTGCGTGCAG
    AGCGTCAGCA AGGGGATGCA GGCTAGCAAT CTTATGCAGC GGCTGAGGAA CCGAGATCGT
    GAGCGTGAGA GGAGGAGCGG AGGACTGAGA GCTGCATCCA GACGAGATCG TGACAGGGAC
    TCGCGGAGGC AGTTGTCCAT CGACACCCGG CCGTTCAGAC CTGCATCAGA AGGAAACCCC
    AGTGATGAAC CAGAACCGCT GCCGGCTCAC AGACAGGCTC TGGGAGAGAG ACTTTACCCA
    CGAGTTCATG CCATGCAACC GGCGTTTGCT AGTAAAATCA CAGGGATGCT GCTGGAACTG
    TCTCCCGCTC AGCTGCTGCT ACTCCTGGCC AGCGAAGACT CGCTCAGAGC CCGAGTGGAG
    GAGGCCATGG AGCTGCTCAT AACTCATGGA CGGGAAAATG GTGCTGACAG TATACTAGAC
    CTCGGCCTCC TCGACACTCC TGAGAAAGCA CAACAGGAGA ACCGAAAGAG GCATGGTTCG
    ACTCGCAGTG TGGTGGACAT GGAGCTGGAT GACCCTGATG ATGGGGATGA TAACGCTCCT
    CTGTTTTACC AGCCTGGAAA GAGGGGTTTC TACTCACCAC GTCCCGGCAA GAACACTGAG
    GCCAGGCTCA ACTGTTTCCG TAACATCGGC AGGATACTGG GTTTGTGTCT GCTACAGAAT
    GAGCTTTGCC CAATCACTCT CAACAGACAC GTCATCAAGG TGCTGCTCGG CAGAAAGGTG
    AACTGGCACG ACTTTGCGTT CTTCGACCCG GTAATGTACG AGAGTTTGCG GCAGCTCATC
    CGTCACTCTC AGACTGAAGA GGCGGAGGCT GTGTTTGCGG CGATGGACCT GGCCTTCGCT
    ATCGACCTCT GCAAGGAGGA GGGAGCAGGA CAGGTGGAGC TGCTGTCCGG TGGAGTCAAC
    ATGCCGGTGA CGCCTCTGAA TGTGTACGAG TACGTGAGGA GATACGCAGA GCACCGGATG
    CTGGTGGTGG CAGAACAGCC TCTGCATGCC ATGCGGAAAG GTCTTCTGGA TGTGCTTCCT
    AAGAACGCTC TGGAAGATCT GACAGCGGAG GATTTCAGAC TGCTGGTCAA CGGCTGTGGA
    GAAGTCAACG TCCAGATGCT CATTAGTTTC ACTTCATTCA ATGATGAGTC AGGTGAGAAT
    GCAGAGAAGC TGCTGCAGTT CAAGCGCTGG TTTTGGTCCA TCGTTGAGAA GATGAGCATG
    ACGGAAAGGC AGGACCTGGT GTACTTCTGG ACGTCCAGCC CGTCTCTGCC GGCCAGTGAA
    GAAGGTTTCC AGCCGATGCC CTCCATCACC ATCCGCCCGC CGGACGACCA GCACCTGCCC
    ACAGCCAACA CCTGCATCTC TCGCCTCTAC GTGCCACTTT ACTCTTCCAA ACAGATCCTC
    AAACAGAAAC TTTTACTAGC CATCAAGACC AAGAACTTCG GTTTTGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22032
    Clone ID Related Accession (Same CDS sequence) XM_005158440.3 , XM_005158440.4
    Accession Version XM_005158440.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8451bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase UBR5 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158440.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGAGA GAGACTCTGA GCTGCTCCGC GAGCGTGAGT CGGTCCTGAG GCTGCGGGAG
    AGGCGGTGGC TGGACGGGGC CTCCTTCGAT GCGGAGCGTG GCTCCACCAG CCGCGAGGGA
    GAGCCCAGCC TTGACAAGAA GAGTGTCCCG CTGCAGAGCC CAGTCACTCT GGGGGAAGAG
    CTCCAGTGGT GGCCTGATAA GGACTATGTA ACAAAGTTTG TCAGCATTGG TGCTTTGTAC
    TCGGAGCTGG TTGCCGTCAG TACCAAAGGA GAACTCTACC AGTGGAAGTG GAATGAACCT
    GAACCTTACA GAAACGCACA AAATCCTTCT ATACATCACC CTCGTGTTCC TTTCCTGGGC
    TTGACCAATG AAAAGATCAC CCACCTGTCC GCCAACAGTA TCCGAGCCAC TGTGGCCACC
    GAGAACAACA AGGTGGCAAC ATGGGTGGAT GAAACTCTGA GCACTGTTGC TGCTAAACTC
    GAACACGGAG CACAAACGTT CCCGGAGCTG CAGGGGGAGC GGATCGTCTC TCTGCACTGC
    TGTGCCCTGT ATACCTGCGC TCAGCTGGAG AACAGCCTTT ACTGGTGGGG TGTTGTGCCT
    TTTAGTCAAC GGAAAAAAAT GCTTGAAAAG GCCAGAGCCA AGAACAAGAA ACCAAAGTCC
    AGTGCTGGAA TCTCTTCGAT ACCCAACATC ACAGTAGGAA CTCAGGTGTG CCTGAGGAAT
    AATCCTCTTT ATCACGCTGG TGCCGTGGCC TTTTCCGTGA ACGCCGGGAT CCCTAAAGTG
    GGCGTCCTGC TGGAGTCGGT GTGGAACATG AACGACAGCT GCCGCTTCCA GCTGCGCTCG
    CCAGAGAGCC TGAAAAACAT GGAGAAAAAC ACCAAGACAC AGGAGACCAA GACGGAGAGC
    AAGCCAGAGC TGGTGAAGAC GGAAATGGGC CCTCCGCCCT CACCAGCGTC CACATGCAGT
    GACACTTCAT CCATCGCTAG CAGCGCTTCA CTGCCGTACA AGCGAAGGCG CTCAACACCG
    GCTCCGAAGG AGGAGGAGAA GGTGAATGAG GAGCAGTGGC CTCTGCGAGA GGTTGTTTTC
    GTGGAGGATG TTAAAAACGT CCCCGTGGGA AAGGTGCTAA AAGTCGATGG TGCATATGTT
    GCTGTGAAAT TTCCAGGGAC GTCGAGCAGC GTGAGCAGCC AGAGCGCAGC TCCCACTGAC
    TCTGACCCGT CCTCACTGCT GCAAGACTGT CGACTGCTCA GAATAGATGA GCTACAGGTT
    GTTAAAACTG GTGGAACACC AAAAGTTCCT GACTGTTTTC AGCGAACACC TAAAAAACTT
    TGCATCCCAG AAAAAGCTGA AATTTTAGCA GTGAATGTTG ACTCCAAAGG AGTTCATGCT
    GTGTTGAAAA CGGGCAGCTG GGTGAGATAC TGTGTCTTTG ACTTAGCCAC CGGCAAAGCC
    GAGCAAGAGA ATCACTTTCC CACCAGTAAC CTGGCGTTTC TTGGTCAGAG CGAGCGAAAT
    GTGGCCATCT TCACTGCTGG CCAGGAGAGT CCCATTATTT TGAGGGATGG CAATGGCACT
    ATTTATCCCA TGGCTAAAGA CTGTATGGGT GGAATACGTG ACCCTGATTG GCTGGATCTG
    CCGCCTATAG CCAGTCTGGG CATGGGAGTT CACTCGCTCG CCAACCTTCC CAGTAACTCC
    ACAATCAAAA AGAAGGCGGC CATCATCATA ATGGCTGTAG AGAAGCAGAC TCTGATGCAG
    CACGTGCTGC GCTGTGATTA CGAGGCGTGT AGGCAGTATC TGATCAGTCT GGAGCAGGCC
    ATGCTACTGG AGCAGAACCC TCACGCTCTG GACACTCTGC TAGGACACCG CTGTGACGGA
    AACCGCAACG TCCTCCATGC CTGTGTGTCC GTCTGCTTCC CCGTCAGCAA CAAGGAAACC
    AAGGAGGAAG AAGAAGCAGA GCGGTCTGAA AGAAACACGT TCGCTGAGAG GCTTTCTGCA
    GTGGAGGCCA TTGCCAATGC CATATCAGTG GTGTCGAGCA ACAGCTCCGG GAACCGGACA
    GGATCATCCA GCAGCAGGGG CTTGCGTTTG AGAGAGATGA TGCGGCGCTC ACTCCGTGCT
    GCAGGTTTAG GCCGACATGA ATCCGGACCT TCCTCCAGCG ACCATCAAGA CCCCGTTTCA
    CCCCCGATAG CACCTCCTAG CTGGGTACCA GACCCTCCAC CGATGGATCC TGACGGTGAC
    ATTGATTTCA TCTTGGCCCC TGCAGTGGGC TCCCTCACCA CTGCTTCCAC AGGCACCAGC
    CAGGGACCCA GCACTTCCAC CATCCCAGGA CCCTCCTCTG AACCATCAGT AGTGGAATCT
    AAAGACCGTA AGGCGAACGC ACACCTGATA CTGAAGCTGA TGTGTGACAG CATCGTCCTG
    CGGCCTCATC TAAGAGAACT GCTCTCTGCC AAGGATGCTC GTGGAATGAC TCCATTCATG
    TTAGCAGTGA GTGGCAGAGC TTACCCAGCA GCCATTACTG TCTTAGAAGC AGCACAGAAA
    ATTGCCAAAG GTGAACCAGG TCTTGGAGAG AAAGAGGATA CAGCATCTGT GTTCATGGAG
    ATGATCTGTC CTTCAGGAAC CAATCCAGAC GACTCTCCGC TGTACGTCCT GTGCTGTAAC
    GACACCTGCA GCTTCACCTG GACTGGAGCA GAACACATCA ACCAGGACAT CTTTGAGTGC
    AGAACTTGTG GCCTGCTGGA GTCGCTGTGC TGCTGCACTG AATGTGCTAG AGTCTGTCAC
    AAGGGGCACG ACTGCAAACT GAAGAGGACG TCACCAACAG CTTACTGTGA CTGCTGGGAG
    AAGTGCAAAT GCAAGACTCT CATCGCAGGC CAGAAAGCGG CACGTCTGGA TCTGCTCTAC
    AGGCTGCTGA CCACCACTAA TCTAGTGACC ACTCCTAACA GCAGGGGAGA GCACATCTTG
    CTGTTTCTGG TGCAGACTGT TGCAAGGCAG AGTGTCGAAC ACTGTCAGTA TCGACCTCCT
    CGCATTCGAG AGGACAGGAA CCGGAAAGCT GCCAGTGCTG AAGACTCTGA TATGCCAGAT
    CATGACCTGG AACCTCCACG CTTTGCTCAG CTGGCTCTGG AGCGAGTGTT GCAGGACTGG
    AACGCACTGA AGTCAATGAT AATGTTCGGC TCTCAGGAAA ATAAAGATCC GTTAAGCGCT
    AGCAGCAGAA TCGCTCACCT TTTACCGGAG GAGCAGATGT ACCTCAACCA GCAGAGTGGC
    ACCATCAGAC TGGACTGTTT CACACACTGC CTTATTGTCA AGTGTGCACC AGACATCACT
    TTCATTGACA CACTGTTAGG GACCCTGGTG AAGGAGCTGC AGAATAAATA CACCCCCGGC
    CGGCGGGAAG AGGCCATCAA TGTCACACGG AGGTTCTTGC GCTCTGTGGC CAGAGTGTTT
    GTCATCCTCA GCGTGGAGAT GGCATCTTCC AAGAAAAAGA ACAACTTCAT CCCTCAGCCT
    ATTGGGAAGT GCAGGCGTGT GTTCCAGGCA CTGTTGCCGT ACGCAGTGGA GGAGCTCTGC
    AATGTGGCCG AGTCTCTGAT CGTGCCGGTG CGTATGGGCA TCGCTCGGCC CACAGCTCCC
    TTCACTCTGG CCAGTACCAG CATTGATGCA GTGCAGGGCA GTGAGGAGCT CTTCTCTGTG
    GAGCCTCTGC CACCTCGACC CTCCCCGGAC CAGTCCAGCA GCTCCAGTCA GTCTGCCTCT
    TCATACATCA TCAGGAACCC GCAGCCCCGC CGCAGCAGCC AGTCTCAGAC AGCCCGCGGA
    CGGGACGAGG AGCAGGATGA TATCGTGTCT GCTGACGTAG AAGAGGTGGA GGTGGTGGAG
    GGTGTTGCAG GTGAGGAGGA TCACCATGAT GACCAGGAGG AGCAGGGAGA GGAGAACGCA
    GAGGCTGAAG GACAGCATGA TGAGCACGAT GAGGATGGGA GCGACATGGA GCTGGATTTA
    TTGGCTGCCG CTGAGACCGA GAGTGACAGT GAGAGTAACC ATAGCAACCA GGACAATGCG
    AGTGGCCGCA GGAGTGTTGT GACGGCAGCC ACTGCTGGAT CTGAAGCAGG TGCCAGCAGT
    GTTCCTGCTT TCTTCTCCGA GGACGACTCC CAGTCGAATG ACTCCAGCGA CTCGGACAGC
    AGCAGCAGCC AGAGTGACGA TGTGGATCAG GAGACGTTTC TGCTGGACGA GCCTCTTGAG
    AGAACCACCG GCTCAGCACA TGCTAACAGT GCAGCCCAGG CCCCACGCTC CATGCAGTGG
    GCTGTTCGCA CTACACCCAG CCAGCGCTCT GGAGGTGGCG CCCCATCCAG CTCGTCCGCT
    CCTGCTGCGA GCTCCACTGG CCTCATCTAC ATCGACCCGT CAAACCTGCG CCGCAGCAGC
    GCCATCAGCA CCAGCGCAGC CGCTGCCGCA GCAGCCCTGG AGGCCAGTAA CTCCAGCAGT
    TATCTGACGT CTGCCAGCAG CCTGGCTCGA GCATACAGCA TCGTCATCCG CCAGATCTCT
    GACCTCATGA GTCTCATTCC CAAATACAAC CACCTGGTCT ACTCGCAGTA CCCCGCTGCT
    GTCAAACTCA CCTATCAGGA TGCTGTCAAT CTGCAGAACT TTGTGGAGGA CAAGTTGATT
    CCCACCTGGA ACTGGATGGT GTCCATCATG GACTCCACTG AAGCGCAGCT GCGTTACGGC
    TCGGCTCTGT CTTCAGCCGG GGATCCTGGT CATCCCAGCC ACCCGCTACA CGCATCCCAG
    CATGCCGGCC GCAGAGAGCG CATGACCGCT CGAGAGGAGG CCAGTCTGCG CACTCTAGAA
    GGACGAAGGC GTGCTGCTAC GCTTCTGACG GCACGTCAGG GCATGATGTC AGCGCGAGGA
    GATTTCCTGA ACTACGCTCT GTCTCTGATG CGTTCCCATA ATGACGAGCA TTCAGACGTT
    CTGCCTGTGT TAGATGTCTG TTCGCTCAAA CACGTGGCTT ACGTCTTCCA GGCGCTCATC
    TACTGGATTA AAGCCATGAA TCTGCAGACC ACGCTCGACA CCACTCAGAT TGACAGGAAG
    AGGAATCGTG AGCTGCTGGA GCTCGGTCTG GACAATGAAG ACTCTGAGCA CGAGAACGAT
    GAGGACACCA ATCAGAGCTC TACACTGCAG GATAAGGACG AGGAGCCTGT GCCTGCAGAG
    ACTGGACAAC ACCATCCGTT TTTCCGCCGC TCGGACTCCA TGACCTTCCT GGGGTGCATC
    CCTCCAAACC CTTTTGAAGT TCCTCTAGCA GAGGCCATTC CTCTGGCTGA CCAGCCGCAT
    CTCCTGCAGC CCAATGCCAG AAAGGAGGAT TTGTTTGGAC GGCCTAGTCA AGGTCTATAT
    TCGTCCTCAT ATATGGCCAG TAAAGGCCTC ACCGATCTGA CTGTGGACAT GAATTGCTTG
    CAGATTCTAC CCACAAAGAT GTCGTATTCG GCGAACATGA AGAACGTGAT GAGTATGGAG
    TCCCGGCAGA GGGGTGGCGA GGAGCAGCCG GTGGCGGAGC AGGAGATGGA TGTGTCTAAA
    CCCGGCCCTT CACCACACGA TCTGGCGGCC CAGTTAAAGA GCAGCCTGCT GGCAGAGATT
    GGCCTGACTG AGAGTGATGG CCCACCGCTG CCCACGTTTA TTCCACACTG TAGTTTTATG
    GGGATGGTGA TCTCTCATGA CATGCTGCTG GGCCGCTGGC GGCTTTCTCT GGAGCTGTTT
    GGCCGTGTTT TCATGGAGGA CGTTGGAGCA GAACCAGGAT CTATCCTGAC AGAGCTGGGG
    GGTTTTGAGG TAAAGGAGTC CAAGTTTCGT CGTGAGATGG AGAAGCTGCG TAACCTTCAG
    TCGCGTGATC TGGCCCTGGA GGTGGACCGA GACCGTGAGC AGCTCATCCA GCAGACCATG
    CGGCAGCTGA ACGCTCACTT CGGCCGCCGC TGTACCACCA CACCCATGGC CGTGCACCGC
    GTAAAGGTGA CATTCAAGGA TGAGCCTGGA GAGGGCAGTG GTGTCGCTCG CAGCTTCTAC
    ACCGCCATCG CTCAGGCCTT CTTGTCCAAC GACAAGCTGC CCAATCTGGA CTGCGTGCAG
    AGCGTCAGCA AGGGGATGCA GGCTAGCAAT CTTATGCAGC GGCTGAGGAA CCGAGATCGT
    GAGCGTGAGA GGAGGAGCGG AGGACTGAGA GCTGCATCCA GACGAGATCG TGACAGGGAC
    TCGCGGAGGC AGTTGTCCAT CGACACCCGG CCGTTCAGAC CTGCATCAGA AGGAAACCCC
    AGTGATGAAC CAGAACCGCT GCCGGCTCAC AGACAGGCTC TGGGAGAGAG ACTTTACCCA
    CGAGTTCATG CCATGCAACC GGCGTTTGCT AGTAAAATCA CAGGGATGCT GCTGGAACTG
    TCTCCCGCTC AGCTGCTGCT ACTCCTGGCC AGCGAAGACT CGCTCAGAGC CCGAGTGGAG
    GAGGCCATGG AGCTGCTCAT AACTCATGGA CGGGAAAATG GTGCTGACAG TATACTAGAC
    CTCGGCCTCC TCGACACTCC TGAGAAAGCA CAACAGGAGA ACCGAAAGAG GCATGGTTCG
    ACTCGCAGTG TGGTGGACAT GGAGCTGGAT GACCCTGATG ATGGGGATGA TAACGCTCCT
    CTGTTTTACC AGCCTGGAAA GAGGGGTTTC TACTCACCAC GTCCCGGCAA GAACACTGAG
    GCCAGGCTCA ACTGTTTCCG TAACATCGGC AGGATACTGG GTTTGTGTCT GCTACAGAAT
    GAGCTTTGCC CAATCACTCT CAACAGACAC GTCATCAAGG TGCTGCTCGG CAGAAAGGTG
    AACTGGCACG ACTTTGCGTT CTTCGACCCG GTAATGTACG AGAGTTTGCG GCAGCTCATC
    CGTCACTCTC AGACTGAAGA GGCGGAGGCT GTGTTTGCGG CGATGGACCT GGCCTTCGCT
    ATCGACCTCT GCAAGGAGGA GGGAGCAGGA CAGGTGGAGC TGCTGTCCGG TGGAGTCAAC
    ATGCCGGTGA CGCCTCTGAA TGTGTACGAG TACGTGAGGA GATACGCAGA GCACCGGATG
    CTGGTGGTGG CAGAACAGCC TCTGCATGCC ATGCGGAAAG GTCTTCTGGA TGTGCTTCCT
    AAGAACGCTC TGGAAGATCT GACAGCGGAG GATTTCAGAC TGCTGGTCAA CGGCTGTGGA
    GAAGTCAACG TCCAGATGCT CATTAGTTTC ACTTCATTCA ATGATGAGTC AGGTGAGAAT
    GCAGAGAAGC TGCTGCAGTT CAAGCGCTGG TTTTGGTCCA TCGTTGAGAA GATGAGCATG
    ACGGAAAGGC AGGACCTGGT GTACTTCTGG ACGTCCAGCC CGTCTCTGCC GGCCAGTGAA
    GAAGGTTTCC AGCCGATGCC CTCCATCACC ATCCGCCCGC CGGACGACCA GCACCTGCCC
    ACAGCCAACA CCTGCATCTC TCGCCTCTAC GTGCCACTTT ACTCTTCCAA ACAGATCCTC
    AAACAGAAAC TTTTACTAGC CATCAAGACC AAGAACTTCG GTTTTGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158497.1
    CDS173..8623
    Translation

    Target ORF information:

    RefSeq Version XM_005158440.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158440.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGTTCA AAGTTGAGCA GTGTCTCAGG GGCAGGAAGG
    AGCTCCAGGC CAGGCAGGAC TAGTGATCCG CCCTGGTTCC TGTCTGGTTC TGACACACTG
    GGCAGACTGG CAGGCAACAC CCTTGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGAGA GAGACTCTGA GCTGCTCCGC GAGCGTGAGT CGGTCCTGAG GCTGCGGGAG
    AGGCGGTGGC TGGACGGGGC CTCCTTCGAT GCGGAGCGTG GCTCCACCAG CCGCGAGGGA
    GAGCCCAGCC TTGACAAGAA GAGTGTCCCG CTGCAGAGCC CAGTCACTCT GGGGGAAGAG
    CTCCAGTGGT GGCCTGATAA GGACTATGTA ACAAAGTTTG TCAGCATTGG TGCTTTGTAC
    TCGGAGCTGG TTGCCGTCAG TACCAAAGGA GAACTCTACC AGTGGAAGTG GAATGAACCT
    GAACCTTACA GAAACGCACA AAATCCTTCT ATACATCACC CTCGTGTTCC TTTCCTGGGC
    TTGACCAATG AAAAGATCAC CCACCTGTCC GCCAACAGTA TCCGAGCCAC TGTGGCCACC
    GAGAACAACA AGGTGGCAAC ATGGGTGGAT GAAACTCTGA GCACTGTTGC TGCTAAACTC
    GAACACGGAG CACAAACGTT CCCGGAGCTG CAGGGGGAGC GGATCGTCTC TCTGCACTGC
    TGTGCCCTGT ATACCTGCGC TCAGCTGGAG AACAGCCTTT ACTGGTGGGG TGTTGTGCCT
    TTTAGTCAAC GGAAAAAAAT GCTTGAAAAG GCCAGAGCCA AGAACAAGAA ACCAAAGTCC
    AGTGCTGGAA TCTCTTCGAT ACCCAACATC ACAGTAGGAA CTCAGGTGTG CCTGAGGAAT
    AATCCTCTTT ATCACGCTGG TGCCGTGGCC TTTTCCGTGA ACGCCGGGAT CCCTAAAGTG
    GGCGTCCTGC TGGAGTCGGT GTGGAACATG AACGACAGCT GCCGCTTCCA GCTGCGCTCG
    CCAGAGAGCC TGAAAAACAT GGAGAAAAAC ACCAAGACAC AGGAGACCAA GACGGAGAGC
    AAGCCAGAGC TGGTGAAGAC GGAAATGGGC CCTCCGCCCT CACCAGCGTC CACATGCAGT
    GACACTTCAT CCATCGCTAG CAGCGCTTCA CTGCCGTACA AGCGAAGGCG CTCAACACCG
    GCTCCGAAGG AGGAGGAGAA GGTGAATGAG GAGCAGTGGC CTCTGCGAGA GGTTGTTTTC
    GTGGAGGATG TTAAAAACGT CCCCGTGGGA AAGGTGCTAA AAGTCGATGG TGCATATGTT
    GCTGTGAAAT TTCCAGGGAC GTCGAGCAGC GTGAGCAGCC AGAGCGCAGC TCCCACTGAC
    TCTGACCCGT CCTCACTGCT GCAAGACTGT CGACTGCTCA GAATAGATGA GCTACAGGTT
    GTTAAAACTG GTGGAACACC AAAAGTTCCT GACTGTTTTC AGCGAACACC TAAAAAACTT
    TGCATCCCAG AAAAAGCTGA AATTTTAGCA GTGAATGTTG ACTCCAAAGG AGTTCATGCT
    GTGTTGAAAA CGGGCAGCTG GGTGAGATAC TGTGTCTTTG ACTTAGCCAC CGGCAAAGCC
    GAGCAAGAGA ATCACTTTCC CACCAGTAAC CTGGCGTTTC TTGGTCAGAG CGAGCGAAAT
    GTGGCCATCT TCACTGCTGG CCAGGAGAGT CCCATTATTT TGAGGGATGG CAATGGCACT
    ATTTATCCCA TGGCTAAAGA CTGTATGGGT GGAATACGTG ACCCTGATTG GCTGGATCTG
    CCGCCTATAG CCAGTCTGGG CATGGGAGTT CACTCGCTCG CCAACCTTCC CAGTAACTCC
    ACAATCAAAA AGAAGGCGGC CATCATCATA ATGGCTGTAG AGAAGCAGAC TCTGATGCAG
    CACGTGCTGC GCTGTGATTA CGAGGCGTGT AGGCAGTATC TGATCAGTCT GGAGCAGGCC
    ATGCTACTGG AGCAGAACCC TCACGCTCTG GACACTCTGC TAGGACACCG CTGTGACGGA
    AACCGCAACG TCCTCCATGC CTGTGTGTCC GTCTGCTTCC CCGTCAGCAA CAAGGAAACC
    AAGGAGGAAG AAGAAGCAGA GCGGTCTGAA AGAAACACGT TCGCTGAGAG GCTTTCTGCA
    GTGGAGGCCA TTGCCAATGC CATATCAGTG GTGTCGAGCA ACAGCTCCGG GAACCGGACA
    GGATCATCCA GCAGCAGGGG CTTGCGTTTG AGAGAGATGA TGCGGCGCTC ACTCCGTGCT
    GCAGGTTTAG GCCGACATGA ATCCGGACCT TCCTCCAGCG ACCATCAAGA CCCCGTTTCA
    CCCCCGATAG CACCTCCTAG CTGGGTACCA GACCCTCCAC CGATGGATCC TGACGGTGAC
    ATTGATTTCA TCTTGGCCCC TGCAGTGGGC TCCCTCACCA CTGCTTCCAC AGGCACCAGC
    CAGGGACCCA GCACTTCCAC CATCCCAGGA CCCTCCTCTG AACCATCAGT AGTGGAATCT
    AAAGACCGTA AGGCGAACGC ACACCTGATA CTGAAGCTGA TGTGTGACAG CATCGTCCTG
    CGGCCTCATC TAAGAGAACT GCTCTCTGCC AAGGATGCTC GTGGAATGAC TCCATTCATG
    TTAGCAGTGA GTGGCAGAGC TTACCCAGCA GCCATTACTG TCTTAGAAGC AGCACAGAAA
    ATTGCCAAAG GTGAACCAGG TCTTGGAGAG AAAGAGGATA CAGCATCTGT GTTCATGGAG
    ATGATCTGTC CTTCAGGAAC CAATCCAGAC GACTCTCCGC TGTACGTCCT GTGCTGTAAC
    GACACCTGCA GCTTCACCTG GACTGGAGCA GAACACATCA ACCAGGACAT CTTTGAGTGC
    AGAACTTGTG GCCTGCTGGA GTCGCTGTGC TGCTGCACTG AATGTGCTAG AGTCTGTCAC
    AAGGGGCACG ACTGCAAACT GAAGAGGACG TCACCAACAG CTTACTGTGA CTGCTGGGAG
    AAGTGCAAAT GCAAGACTCT CATCGCAGGC CAGAAAGCGG CACGTCTGGA TCTGCTCTAC
    AGGCTGCTGA CCACCACTAA TCTAGTGACC ACTCCTAACA GCAGGGGAGA GCACATCTTG
    CTGTTTCTGG TGCAGACTGT TGCAAGGCAG AGTGTCGAAC ACTGTCAGTA TCGACCTCCT
    CGCATTCGAG AGGACAGGAA CCGGAAAGCT GCCAGTGCTG AAGACTCTGA TATGCCAGAT
    CATGACCTGG AACCTCCACG CTTTGCTCAG CTGGCTCTGG AGCGAGTGTT GCAGGACTGG
    AACGCACTGA AGTCAATGAT AATGTTCGGC TCTCAGGAAA ATAAAGATCC GTTAAGCGCT
    AGCAGCAGAA TCGCTCACCT TTTACCGGAG GAGCAGATGT ACCTCAACCA GCAGAGTGGC
    ACCATCAGAC TGGACTGTTT CACACACTGC CTTATTGTCA AGTGTGCACC AGACATCACT
    TTCATTGACA CACTGTTAGG GACCCTGGTG AAGGAGCTGC AGAATAAATA CACCCCCGGC
    CGGCGGGAAG AGGCCATCAA TGTCACACGG AGGTTCTTGC GCTCTGTGGC CAGAGTGTTT
    GTCATCCTCA GCGTGGAGAT GGCATCTTCC AAGAAAAAGA ACAACTTCAT CCCTCAGCCT
    ATTGGGAAGT GCAGGCGTGT GTTCCAGGCA CTGTTGCCGT ACGCAGTGGA GGAGCTCTGC
    AATGTGGCCG AGTCTCTGAT CGTGCCGGTG CGTATGGGCA TCGCTCGGCC CACAGCTCCC
    TTCACTCTGG CCAGTACCAG CATTGATGCA GTGCAGGGCA GTGAGGAGCT CTTCTCTGTG
    GAGCCTCTGC CACCTCGACC CTCCCCGGAC CAGTCCAGCA GCTCCAGTCA GTCTGCCTCT
    TCATACATCA TCAGGAACCC GCAGCCCCGC CGCAGCAGCC AGTCTCAGAC AGCCCGCGGA
    CGGGACGAGG AGCAGGATGA TATCGTGTCT GCTGACGTAG AAGAGGTGGA GGTGGTGGAG
    GGTGTTGCAG GTGAGGAGGA TCACCATGAT GACCAGGAGG AGCAGGGAGA GGAGAACGCA
    GAGGCTGAAG GACAGCATGA TGAGCACGAT GAGGATGGGA GCGACATGGA GCTGGATTTA
    TTGGCTGCCG CTGAGACCGA GAGTGACAGT GAGAGTAACC ATAGCAACCA GGACAATGCG
    AGTGGCCGCA GGAGTGTTGT GACGGCAGCC ACTGCTGGAT CTGAAGCAGG TGCCAGCAGT
    GTTCCTGCTT TCTTCTCCGA GGACGACTCC CAGTCGAATG ACTCCAGCGA CTCGGACAGC
    AGCAGCAGCC AGAGTGACGA TGTGGATCAG GAGACGTTTC TGCTGGACGA GCCTCTTGAG
    AGAACCACCG GCTCAGCACA TGCTAACAGT GCAGCCCAGG CCCCACGCTC CATGCAGTGG
    GCTGTTCGCA CTACACCCAG CCAGCGCTCT GGAGGTGGCG CCCCATCCAG CTCGTCCGCT
    CCTGCTGCGA GCTCCACTGG CCTCATCTAC ATCGACCCGT CAAACCTGCG CCGCAGCAGC
    GCCATCAGCA CCAGCGCAGC CGCTGCCGCA GCAGCCCTGG AGGCCAGTAA CTCCAGCAGT
    TATCTGACGT CTGCCAGCAG CCTGGCTCGA GCATACAGCA TCGTCATCCG CCAGATCTCT
    GACCTCATGA GTCTCATTCC CAAATACAAC CACCTGGTCT ACTCGCAGTA CCCCGCTGCT
    GTCAAACTCA CCTATCAGGA TGCTGTCAAT CTGCAGAACT TTGTGGAGGA CAAGTTGATT
    CCCACCTGGA ACTGGATGGT GTCCATCATG GACTCCACTG AAGCGCAGCT GCGTTACGGC
    TCGGCTCTGT CTTCAGCCGG GGATCCTGGT CATCCCAGCC ACCCGCTACA CGCATCCCAG
    CATGCCGGCC GCAGAGAGCG CATGACCGCT CGAGAGGAGG CCAGTCTGCG CACTCTAGAA
    GGACGAAGGC GTGCTGCTAC GCTTCTGACG GCACGTCAGG GCATGATGTC AGCGCGAGGA
    GATTTCCTGA ACTACGCTCT GTCTCTGATG CGTTCCCATA ATGACGAGCA TTCAGACGTT
    CTGCCTGTGT TAGATGTCTG TTCGCTCAAA CACGTGGCTT ACGTCTTCCA GGCGCTCATC
    TACTGGATTA AAGCCATGAA TCTGCAGACC ACGCTCGACA CCACTCAGAT TGACAGGAAG
    AGGAATCGTG AGCTGCTGGA GCTCGGTCTG GACAATGAAG ACTCTGAGCA CGAGAACGAT
    GAGGACACCA ATCAGAGCTC TACACTGCAG GATAAGGACG AGGAGCCTGT GCCTGCAGAG
    ACTGGACAAC ACCATCCGTT TTTCCGCCGC TCGGACTCCA TGACCTTCCT GGGGTGCATC
    CCTCCAAACC CTTTTGAAGT TCCTCTAGCA GAGGCCATTC CTCTGGCTGA CCAGCCGCAT
    CTCCTGCAGC CCAATGCCAG AAAGGAGGAT TTGTTTGGAC GGCCTAGTCA AGGTCTATAT
    TCGTCCTCAT ATATGGCCAG TAAAGGCCTC ACCGATCTGA CTGTGGACAT GAATTGCTTG
    CAGATTCTAC CCACAAAGAT GTCGTATTCG GCGAACATGA AGAACGTGAT GAGTATGGAG
    TCCCGGCAGA GGGGTGGCGA GGAGCAGCCG GTGGCGGAGC AGGAGATGGA TGTGTCTAAA
    CCCGGCCCTT CACCACACGA TCTGGCGGCC CAGTTAAAGA GCAGCCTGCT GGCAGAGATT
    GGCCTGACTG AGAGTGATGG CCCACCGCTG CCCACGTTTA TTCCACACTG TAGTTTTATG
    GGGATGGTGA TCTCTCATGA CATGCTGCTG GGCCGCTGGC GGCTTTCTCT GGAGCTGTTT
    GGCCGTGTTT TCATGGAGGA CGTTGGAGCA GAACCAGGAT CTATCCTGAC AGAGCTGGGG
    GGTTTTGAGG TAAAGGAGTC CAAGTTTCGT CGTGAGATGG AGAAGCTGCG TAACCTTCAG
    TCGCGTGATC TGGCCCTGGA GGTGGACCGA GACCGTGAGC AGCTCATCCA GCAGACCATG
    CGGCAGCTGA ACGCTCACTT CGGCCGCCGC TGTACCACCA CACCCATGGC CGTGCACCGC
    GTAAAGGTGA CATTCAAGGA TGAGCCTGGA GAGGGCAGTG GTGTCGCTCG CAGCTTCTAC
    ACCGCCATCG CTCAGGCCTT CTTGTCCAAC GACAAGCTGC CCAATCTGGA CTGCGTGCAG
    AGCGTCAGCA AGGGGATGCA GGCTAGCAAT CTTATGCAGC GGCTGAGGAA CCGAGATCGT
    GAGCGTGAGA GGAGGAGCGG AGGACTGAGA GCTGCATCCA GACGAGATCG TGACAGGGAC
    TCGCGGAGGC AGTTGTCCAT CGACACCCGG CCGTTCAGAC CTGCATCAGA AGGAAACCCC
    AGTGATGAAC CAGAACCGCT GCCGGCTCAC AGACAGGCTC TGGGAGAGAG ACTTTACCCA
    CGAGTTCATG CCATGCAACC GGCGTTTGCT AGTAAAATCA CAGGGATGCT GCTGGAACTG
    TCTCCCGCTC AGCTGCTGCT ACTCCTGGCC AGCGAAGACT CGCTCAGAGC CCGAGTGGAG
    GAGGCCATGG AGCTGCTCAT AACTCATGGA CGGGAAAATG GTGCTGACAG TATACTAGAC
    CTCGGCCTCC TCGACACTCC TGAGAAAGCA CAACAGGAGA ACCGAAAGAG GCATGGTTCG
    ACTCGCAGTG TGGTGGACAT GGAGCTGGAT GACCCTGATG ATGGGGATGA TAACGCTCCT
    CTGTTTTACC AGCCTGGAAA GAGGGGTTTC TACTCACCAC GTCCCGGCAA GAACACTGAG
    GCCAGGCTCA ACTGTTTCCG TAACATCGGC AGGATACTGG GTTTGTGTCT GCTACAGAAT
    GAGCTTTGCC CAATCACTCT CAACAGACAC GTCATCAAGG TGCTGCTCGG CAGAAAGGTG
    AACTGGCACG ACTTTGCGTT CTTCGACCCG GTAATGTACG AGAGTTTGCG GCAGCTCATC
    CGTCACTCTC AGACTGAAGA GGCGGAGGCT GTGTTTGCGG CGATGGACCT GGCCTTCGCT
    ATCGACCTCT GCAAGGAGGA GGGAGCAGGA CAGGTGGAGC TGCTGTCCGG TGGAGTCAAC
    ATGCCGGTGA CGCCTCTGAA TGTGTACGAG TACGTGAGGA GATACGCAGA GCACCGGATG
    CTGGTGGTGG CAGAACAGCC TCTGCATGCC ATGCGGAAAG GTCTTCTGGA TGTGCTTCCT
    AAGAACGCTC TGGAAGATCT GACAGCGGAG GATTTCAGAC TGCTGGTCAA CGGCTGTGGA
    GAAGTCAACG TCCAGATGCT CATTAGTTTC ACTTCATTCA ATGATGAGTC AGGTGAGAAT
    GCAGAGAAGC TGCTGCAGTT CAAGCGCTGG TTTTGGTCCA TCGTTGAGAA GATGAGCATG
    ACGGAAAGGC AGGACCTGGT GTACTTCTGG ACGTCCAGCC CGTCTCTGCC GGCCAGTGAA
    GAAGGTTTCC AGCCGATGCC CTCCATCACC ATCCGCCCGC CGGACGACCA GCACCTGCCC
    ACAGCCAACA CCTGCATCTC TCGCCTCTAC GTGCCACTTT ACTCTTCCAA ACAGATCCTC
    AAACAGAAAC TTTTACTAGC CATCAAGACC AAGAACTTCG GTTTTGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa22033
    Clone ID Related Accession (Same CDS sequence) XM_005158441.3
    Accession Version XM_005158441.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8349bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase UBR5 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158441.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGC
    TCCAGTCAGT CTGCCTCTTC ATACATCATC AGGAACCCGC AGCCCCGCCG CAGCAGCCAG
    TCTCAGACAG CCCGCGGACG GGACGAGGAG CAGGATGATA TCGTGTCTGC TGACGTAGAA
    GAGGTGGAGG TGGTGGAGGG TGTTGCAGGT GAGGAGGATC ACCATGATGA CCAGGAGGAG
    CAGGGAGAGG AGAACGCAGA GGCTGAAGGA CAGCATGATG AGCACGATGA GGATGGGAGC
    GACATGGAGC TGGATTTATT GGCTGCCGCT GAGACCGAGA GTGACAGTGA GAGTAACCAT
    AGCAACCAGG ACAATGCGAG TGGCCGCAGG AGTGTTGTGA CGGCAGCCAC TGCTGGATCT
    GAAGCAGGTG CCAGCAGTGT TCCTGCTTTC TTCTCCGAGG ACGACTCCCA GTCGAATGAC
    TCCAGCGACT CGGACAGCAG CAGCAGCCAG AGTGACGATG TGGATCAGGA GACGTTTCTG
    CTGGACGAGC CTCTTGAGAG AACCACCGGC TCAGCACATG CTAACAGTGC AGCCCAGGCC
    CCACGCTCCA TGCAGTGGGC TGTTCGCACT ACACCCAGCC AGCGCTCTGG AGGTGGCGCC
    CCATCCAGCT CGTCCGCTCC TGCTGCGAGC TCCACTGGCC TCATCTACAT CGACCCGTCA
    AACCTGCGCC GCAGCAGCGC CATCAGCACC AGCGCAGCCG CTGCCGCAGC AGCCCTGGAG
    GCCAGTAACT CCAGCAGTTA TCTGACGTCT GCCAGCAGCC TGGCTCGAGC ATACAGCATC
    GTCATCCGCC AGATCTCTGA CCTCATGAGT CTCATTCCCA AATACAACCA CCTGGTCTAC
    TCGCAGTACC CCGCTGCTGT CAAACTCACC TATCAGGATG CTGTCAATCT GCAGAACTTT
    GTGGAGGACA AGTTGATTCC CACCTGGAAC TGGATGGTGT CCATCATGGA CTCCACTGAA
    GCGCAGCTGC GTTACGGCTC GGCTCTGTCT TCAGCCGGGG ATCCTGGTCA TCCCAGCCAC
    CCGCTACACG CATCCCAGCA TGCCGGCCGC AGAGAGCGCA TGACCGCTCG AGAGGAGGCC
    AGTCTGCGCA CTCTAGAAGG ACGAAGGCGT GCTGCTACGC TTCTGACGGC ACGTCAGGGC
    ATGATGTCAG CGCGAGGAGA TTTCCTGAAC TACGCTCTGT CTCTGATGCG TTCCCATAAT
    GACGAGCATT CAGACGTTCT GCCTGTGTTA GATGTCTGTT CGCTCAAACA CGTGGCTTAC
    GTCTTCCAGG CGCTCATCTA CTGGATTAAA GCCATGAATC TGCAGACCAC GCTCGACACC
    ACTCAGATTG ACAGGAAGAG GAATCGTGAG CTGCTGGAGC TCGGTCTGGA CAATGAAGAC
    TCTGAGCACG AGAACGATGA GGACACCAAT CAGAGCTCTA CACTGCAGGA TAAGGACGAG
    GAGCCTGTGC CTGCAGAGAC TGGACAACAC CATCCGTTTT TCCGCCGCTC GGACTCCATG
    ACCTTCCTGG GGTGCATCCC TCCAAACCCT TTTGAAGTTC CTCTAGCAGA GGCCATTCCT
    CTGGCTGACC AGCCGCATCT CCTGCAGCCC AATGCCAGAA AGGAGGATTT GTTTGGACGG
    CCTAGTCAAG GTCTATATTC GTCCTCATAT ATGGCCAGTA AAGGCCTCAC CGATCTGACT
    GTGGACATGA ATTGCTTGCA GATTCTACCC ACAAAGATGT CGTATTCGGC GAACATGAAG
    AACGTGATGA GTATGGAGTC CCGGCAGAGG GGTGGCGAGG AGCAGCCGGT GGCGGAGCAG
    GAGATGGATG TGTCTAAACC CGGCCCTTCA CCACACGATC TGGCGGCCCA GTTAAAGAGC
    AGCCTGCTGG CAGAGATTGG CCTGACTGAG AGTGATGGCC CACCGCTGCC CACGTTTATT
    CCACACTGTA GTTTTATGGG GATGGTGATC TCTCATGACA TGCTGCTGGG CCGCTGGCGG
    CTTTCTCTGG AGCTGTTTGG CCGTGTTTTC ATGGAGGACG TTGGAGCAGA ACCAGGATCT
    ATCCTGACAG AGCTGGGGGG TTTTGAGGTA AAGGAGTCCA AGTTTCGTCG TGAGATGGAG
    AAGCTGCGTA ACCTTCAGTC GCGTGATCTG GCCCTGGAGG TGGACCGAGA CCGTGAGCAG
    CTCATCCAGC AGACCATGCG GCAGCTGAAC GCTCACTTCG GCCGCCGCTG TACCACCACA
    CCCATGGCCG TGCACCGCGT AAAGGTGACA TTCAAGGATG AGCCTGGAGA GGGCAGTGGT
    GTCGCTCGCA GCTTCTACAC CGCCATCGCT CAGGCCTTCT TGTCCAACGA CAAGCTGCCC
    AATCTGGACT GCGTGCAGAG CGTCAGCAAG GGGATGCAGG CTAGCAATCT TATGCAGCGG
    CTGAGGAACC GAGATCGTGA GCGTGAGAGG AGGAGCGGAG GACTGAGAGC TGCATCCAGA
    CGAGATCGTG ACAGGGACTC GCGGAGGCAG TTGTCCATCG ACACCCGGCC GTTCAGACCT
    GCATCAGAAG GAAACCCCAG TGATGAACCA GAACCGCTGC CGGCTCACAG ACAGGCTCTG
    GGAGAGAGAC TTTACCCACG AGTTCATGCC ATGCAACCGG CGTTTGCTAG TAAAATCACA
    GGGATGCTGC TGGAACTGTC TCCCGCTCAG CTGCTGCTAC TCCTGGCCAG CGAAGACTCG
    CTCAGAGCCC GAGTGGAGGA GGCCATGGAG CTGCTCATAA CTCATGGACG GGAAAATGGT
    GCTGACAGTA TACTAGACCT CGGCCTCCTC GACACTCCTG AGAAAGCACA ACAGGAGAAC
    CGAAAGAGGC ATGGTTCGAC TCGCAGTGTG GTGGACATGG AGCTGGATGA CCCTGATGAT
    GGGGATGATA ACGCTCCTCT GTTTTACCAG CCTGGAAAGA GGGGTTTCTA CTCACCACGT
    CCCGGCAAGA ACACTGAGGC CAGGCTCAAC TGTTTCCGTA ACATCGGCAG GATACTGGGT
    TTGTGTCTGC TACAGAATGA GCTTTGCCCA ATCACTCTCA ACAGACACGT CATCAAGGTG
    CTGCTCGGCA GAAAGGTGAA CTGGCACGAC TTTGCGTTCT TCGACCCGGT AATGTACGAG
    AGTTTGCGGC AGCTCATCCG TCACTCTCAG ACTGAAGAGG CGGAGGCTGT GTTTGCGGCG
    ATGGACCTGG CCTTCGCTAT CGACCTCTGC AAGGAGGAGG GAGCAGGACA GGTGGAGCTG
    CTGTCCGGTG GAGTCAACAT GCCGGTGACG CCTCTGAATG TGTACGAGTA CGTGAGGAGA
    TACGCAGAGC ACCGGATGCT GGTGGTGGCA GAACAGCCTC TGCATGCCAT GCGGAAAGGT
    CTTCTGGATG TGCTTCCTAA GAACGCTCTG GAAGATCTGA CAGCGGAGGA TTTCAGACTG
    CTGGTCAACG GCTGTGGAGA AGTCAACGTC CAGATGCTCA TTAGTTTCAC TTCATTCAAT
    GATGAGTCAG GTGAGAATGC AGAGAAGCTG CTGCAGTTCA AGCGCTGGTT TTGGTCCATC
    GTTGAGAAGA TGAGCATGAC GGAAAGGCAG GACCTGGTGT ACTTCTGGAC GTCCAGCCCG
    TCTCTGCCGG CCAGTGAAGA AGGTTTCCAG CCGATGCCCT CCATCACCAT CCGCCCGCCG
    GACGACCAGC ACCTGCCCAC AGCCAACACC TGCATCTCTC GCCTCTACGT GCCACTTTAC
    TCTTCCAAAC AGATCCTCAA ACAGAAACTT TTACTAGCCA TCAAGACCAA GAACTTCGGT
    TTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005158498.1
    CDS234..8582
    Translation

    Target ORF information:

    RefSeq Version XM_005158441.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ubiquitin protein ligase E3 component n-recognin 5 (ubr5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005158441.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGACGTCCA TTCACTTCGT GGTTCACCCG TTACCCGGGA CCGAAGACCA GCTTAATGAC 
    AGACTCCGAG AAGTGTCGGA AAAGCTCAAC AAATACAACT GTAATAGTCA TCCACACCTT
    ATTCCATTGG AGCAGGCCAA ACTTAAGCAG TGTGTGGTTG GACCAAATCA TGCTGGATTT
    CTCCTTGAGG ATGGACGCAT CTGCAGGATA AGCTTTGCCG TTCAGCCTGA CCGTCTGGAG
    CTTGGCAAGC CGGATGGCAA TGATGGGACC CGCTGGAGTT CAGGAGTAAA TGGCGGAGGC
    AGTGGAGGAG GGGGCAGCAG CGGAGGAGGC GCAGGAGGAG GCAGCGGGAG CGGAGGAGGC
    GCTGGAGGAG GCACAGGAGG AGGAGGATCT TCAGGACGTT CATCCACAGC AGCCCGTGAT
    TCACGCAGGC AGACCCGCGT CATCCGCACC GGCAGAGATC GAGGCTCTGG CCTGCTGGGA
    AGCCAACCAC AGCCTGTGAT TCCAGCCTCC GTCATCCCCG AGGAGCTCAT CTCTCAGGCT
    CAGGTGGTTC TTCAGGGGAA ATCCAGAAGT GTCATCATTC GAGAGCTGCA GCGCACAAAC
    CTTGACGTGA ACTTGGCTGT AAACAACCTG CTGAGCAGAG ATGATGAGGA TGGAGATGAT
    GGCGATGACA CGGCCAGCGA ATCCTACTTA CCTGGAGAAG ACTTGATGTC GCTGCTAGAT
    GCAGACATTC ACTCCGCTCA CCCCAGCGTG ATCATAGATG CCGATGCCAT GTTCTCTGAG
    GACATCAGCT ACTTCGGTTA CCCCTCTTTT CGTCGCTCCT CCTTGTCTCG TCTTGGCTCC
    TCACGAGTTC TCCTTCTCCC CCTAGAGAGA GACTCTGAGC TGCTCCGCGA GCGTGAGTCG
    GTCCTGAGGC TGCGGGAGAG GCGGTGGCTG GACGGGGCCT CCTTCGATGC GGAGCGTGGC
    TCCACCAGCC GCGAGGGAGA GCCCAGCCTT GACAAGAAGA GTGTCCCGCT GCAGAGCCCA
    GTCACTCTGG GGGAAGAGCT CCAGTGGTGG CCTGATAAGG ACTATGTAAC AAAGTTTGTC
    AGCATTGGTG CTTTGTACTC GGAGCTGGTT GCCGTCAGTA CCAAAGGAGA ACTCTACCAG
    TGGAAGTGGA ATGAACCTGA ACCTTACAGA AACGCACAAA ATCCTTCTAT ACATCACCCT
    CGTGTTCCTT TCCTGGGCTT GACCAATGAA AAGATCACCC ACCTGTCCGC CAACAGTATC
    CGAGCCACTG TGGCCACCGA GAACAACAAG GTGGCAACAT GGGTGGATGA AACTCTGAGC
    ACTGTTGCTG CTAAACTCGA ACACGGAGCA CAAACGTTCC CGGAGCTGCA GGGGGAGCGG
    ATCGTCTCTC TGCACTGCTG TGCCCTGTAT ACCTGCGCTC AGCTGGAGAA CAGCCTTTAC
    TGGTGGGGTG TTGTGCCTTT TAGTCAACGG AAAAAAATGC TTGAAAAGGC CAGAGCCAAG
    AACAAGAAAC CAAAGTCCAG TGCTGGAATC TCTTCGATAC CCAACATCAC AGTAGGAACT
    CAGGTGTGCC TGAGGAATAA TCCTCTTTAT CACGCTGGTG CCGTGGCCTT TTCCGTGAAC
    GCCGGGATCC CTAAAGTGGG CGTCCTGCTG GAGTCGGTGT GGAACATGAA CGACAGCTGC
    CGCTTCCAGC TGCGCTCGCC AGAGAGCCTG AAAAACATGG AGAAAAACAC CAAGACACAG
    GAGACCAAGA CGGAGAGCAA GCCAGAGCTG GTGAAGACGG AAATGGGCCC TCCGCCCTCA
    CCAGCGTCCA CATGCAGTGA CACTTCATCC ATCGCTAGCA GCGCTTCACT GCCGTACAAG
    CGAAGGCGCT CAACACCGGC TCCGAAGGAG GAGGAGAAGG TGAATGAGGA GCAGTGGCCT
    CTGCGAGAGG TTGTTTTCGT GGAGGATGTT AAAAACGTCC CCGTGGGAAA GGTGCTAAAA
    GTCGATGGTG CATATGTTGC TGTGAAATTT CCAGGGACGT CGAGCAGCGT GAGCAGCCAG
    AGCGCAGCTC CCACTGACTC TGACCCGTCC TCACTGCTGC AAGACTGTCG ACTGCTCAGA
    ATAGATGAGC TACAGGTTGT TAAAACTGGT GGAACACCAA AAGTTCCTGA CTGTTTTCAG
    CGAACACCTA AAAAACTTTG CATCCCAGAA AAAGCTGAAA TTTTAGCAGT GAATGTTGAC
    TCCAAAGGAG TTCATGCTGT GTTGAAAACG GGCAGCTGGG TGAGATACTG TGTCTTTGAC
    TTAGCCACCG GCAAAGCCGA GCAAGAGAAT CACTTTCCCA CCAGTAACCT GGCGTTTCTT
    GGTCAGAGCG AGCGAAATGT GGCCATCTTC ACTGCTGGCC AGGAGAGTCC CATTATTTTG
    AGGGATGGCA ATGGCACTAT TTATCCCATG GCTAAAGACT GTATGGGTGG AATACGTGAC
    CCTGATTGGC TGGATCTGCC GCCTATAGCC AGTCTGGGCA TGGGAGTTCA CTCGCTCGCC
    AACCTTCCCA GTAACTCCAC AATCAAAAAG AAGGCGGCCA TCATCATAAT GGCTGTAGAG
    AAGCAGACTC TGATGCAGCA CGTGCTGCGC TGTGATTACG AGGCGTGTAG GCAGTATCTG
    ATCAGTCTGG AGCAGGCCAT GCTACTGGAG CAGAACCCTC ACGCTCTGGA CACTCTGCTA
    GGACACCGCT GTGACGGAAA CCGCAACGTC CTCCATGCCT GTGTGTCCGT CTGCTTCCCC
    GTCAGCAACA AGGAAACCAA GGAGGAAGAA GAAGCAGAGC GGTCTGAAAG AAACACGTTC
    GCTGAGAGGC TTTCTGCAGT GGAGGCCATT GCCAATGCCA TATCAGTGGT GTCGAGCAAC
    AGCTCCGGGA ACCGGACAGG ATCATCCAGC AGCAGGGGCT TGCGTTTGAG AGAGATGATG
    CGGCGCTCAC TCCGTGCTGC AGGTTTAGGC CGACATGAAT CCGGACCTTC CTCCAGCGAC
    CATCAAGACC CCGTTTCACC CCCGATAGCA CCTCCTAGCT GGGTACCAGA CCCTCCACCG
    ATGGATCCTG ACGGTGACAT TGATTTCATC TTGGCCCCTG CAGTGGGCTC CCTCACCACT
    GCTTCCACAG GCACCAGCCA GGGACCCAGC ACTTCCACCA TCCCAGGACC CTCCTCTGAA
    CCATCAGTAG TGGAATCTAA AGACCGTAAG GCGAACGCAC ACCTGATACT GAAGCTGATG
    TGTGACAGCA TCGTCCTGCG GCCTCATCTA AGAGAACTGC TCTCTGCCAA GGATGCTCGT
    GGAATGACTC CATTCATGTT AGCAGTGAGT GGCAGAGCTT ACCCAGCAGC CATTACTGTC
    TTAGAAGCAG CACAGAAAAT TGCCAAAGGT GAACCAGGTC TTGGAGAGAA AGAGGATACA
    GCATCTGTGT TCATGGAGAT GATCTGTCCT TCAGGAACCA ATCCAGACGA CTCTCCGCTG
    TACGTCCTGT GCTGTAACGA CACCTGCAGC TTCACCTGGA CTGGAGCAGA ACACATCAAC
    CAGGACATCT TTGAGTGCAG AACTTGTGGC CTGCTGGAGT CGCTGTGCTG CTGCACTGAA
    TGTGCTAGAG TCTGTCACAA GGGGCACGAC TGCAAACTGA AGAGGACGTC ACCAACAGCT
    TACTGTGACT GCTGGGAGAA GTGCAAATGC AAGACTCTCA TCGCAGGCCA GAAAGCGGCA
    CGTCTGGATC TGCTCTACAG GCTGCTGACC ACCACTAATC TAGTGACCAC TCCTAACAGC
    AGGGGAGAGC ACATCTTGCT GTTTCTGGTG CAGACTGTTG CAAGGCAGAG TGTCGAACAC
    TGTCAGTATC GACCTCCTCG CATTCGAGAG GACAGGAACC GGAAAGCTGC CAGTGCTGAA
    GACTCTGATA TGCCAGATCA TGACCTGGAA CCTCCACGCT TTGCTCAGCT GGCTCTGGAG
    CGAGTGTTGC AGGACTGGAA CGCACTGAAG TCAATGATAA TGTTCGGCTC TCAGGAAAAT
    AAAGATCCGT TAAGCGCTAG CAGCAGAATC GCTCACCTTT TACCGGAGGA GCAGATGTAC
    CTCAACCAGC AGAGTGGCAC CATCAGACTG GACTGTTTCA CACACTGCCT TATTGTCAAG
    TGTGCACCAG ACATCACTTT CATTGACACA CTGTTAGGGA CCCTGGTGAA GGAGCTGCAG
    AATAAATACA CCCCCGGCCG GCGGGAAGAG GCCATCAATG TCACACGGAG GTTCTTGCGC
    TCTGTGGCCA GAGTGTTTGT CATCCTCAGC GTGGAGATGG CATCTTCCAA GAAAAAGAAC
    AACTTCATCC CTCAGCCTAT TGGGAAGTGC AGGCGTGTGT TCCAGGCACT GTTGCCGTAC
    GCAGTGGAGG AGCTCTGCAA TGTGGCCGAG TCTCTGATCG TGCCGGTGCG TATGGGCATC
    GCTCGGCCCA CAGCTCCCTT CACTCTGGCC AGTACCAGCA TTGATGCAGT GCAGGGCAGT
    GAGGAGCTCT TCTCTGTGGA GCCTCTGCCA CCTCGACCCT CCCCGGACCA GTCCAGCAGC
    TCCAGTCAGT CTGCCTCTTC ATACATCATC AGGAACCCGC AGCCCCGCCG CAGCAGCCAG
    TCTCAGACAG CCCGCGGACG GGACGAGGAG CAGGATGATA TCGTGTCTGC TGACGTAGAA
    GAGGTGGAGG TGGTGGAGGG TGTTGCAGGT GAGGAGGATC ACCATGATGA CCAGGAGGAG
    CAGGGAGAGG AGAACGCAGA GGCTGAAGGA CAGCATGATG AGCACGATGA GGATGGGAGC
    GACATGGAGC TGGATTTATT GGCTGCCGCT GAGACCGAGA GTGACAGTGA GAGTAACCAT
    AGCAACCAGG ACAATGCGAG TGGCCGCAGG AGTGTTGTGA CGGCAGCCAC TGCTGGATCT
    GAAGCAGGTG CCAGCAGTGT TCCTGCTTTC TTCTCCGAGG ACGACTCCCA GTCGAATGAC
    TCCAGCGACT CGGACAGCAG CAGCAGCCAG AGTGACGATG TGGATCAGGA GACGTTTCTG
    CTGGACGAGC CTCTTGAGAG AACCACCGGC TCAGCACATG CTAACAGTGC AGCCCAGGCC
    CCACGCTCCA TGCAGTGGGC TGTTCGCACT ACACCCAGCC AGCGCTCTGG AGGTGGCGCC
    CCATCCAGCT CGTCCGCTCC TGCTGCGAGC TCCACTGGCC TCATCTACAT CGACCCGTCA
    AACCTGCGCC GCAGCAGCGC CATCAGCACC AGCGCAGCCG CTGCCGCAGC AGCCCTGGAG
    GCCAGTAACT CCAGCAGTTA TCTGACGTCT GCCAGCAGCC TGGCTCGAGC ATACAGCATC
    GTCATCCGCC AGATCTCTGA CCTCATGAGT CTCATTCCCA AATACAACCA CCTGGTCTAC
    TCGCAGTACC CCGCTGCTGT CAAACTCACC TATCAGGATG CTGTCAATCT GCAGAACTTT
    GTGGAGGACA AGTTGATTCC CACCTGGAAC TGGATGGTGT CCATCATGGA CTCCACTGAA
    GCGCAGCTGC GTTACGGCTC GGCTCTGTCT TCAGCCGGGG ATCCTGGTCA TCCCAGCCAC
    CCGCTACACG CATCCCAGCA TGCCGGCCGC AGAGAGCGCA TGACCGCTCG AGAGGAGGCC
    AGTCTGCGCA CTCTAGAAGG ACGAAGGCGT GCTGCTACGC TTCTGACGGC ACGTCAGGGC
    ATGATGTCAG CGCGAGGAGA TTTCCTGAAC TACGCTCTGT CTCTGATGCG TTCCCATAAT
    GACGAGCATT CAGACGTTCT GCCTGTGTTA GATGTCTGTT CGCTCAAACA CGTGGCTTAC
    GTCTTCCAGG CGCTCATCTA CTGGATTAAA GCCATGAATC TGCAGACCAC GCTCGACACC
    ACTCAGATTG ACAGGAAGAG GAATCGTGAG CTGCTGGAGC TCGGTCTGGA CAATGAAGAC
    TCTGAGCACG AGAACGATGA GGACACCAAT CAGAGCTCTA CACTGCAGGA TAAGGACGAG
    GAGCCTGTGC CTGCAGAGAC TGGACAACAC CATCCGTTTT TCCGCCGCTC GGACTCCATG
    ACCTTCCTGG GGTGCATCCC TCCAAACCCT TTTGAAGTTC CTCTAGCAGA GGCCATTCCT
    CTGGCTGACC AGCCGCATCT CCTGCAGCCC AATGCCAGAA AGGAGGATTT GTTTGGACGG
    CCTAGTCAAG GTCTATATTC GTCCTCATAT ATGGCCAGTA AAGGCCTCAC CGATCTGACT
    GTGGACATGA ATTGCTTGCA GATTCTACCC ACAAAGATGT CGTATTCGGC GAACATGAAG
    AACGTGATGA GTATGGAGTC CCGGCAGAGG GGTGGCGAGG AGCAGCCGGT GGCGGAGCAG
    GAGATGGATG TGTCTAAACC CGGCCCTTCA CCACACGATC TGGCGGCCCA GTTAAAGAGC
    AGCCTGCTGG CAGAGATTGG CCTGACTGAG AGTGATGGCC CACCGCTGCC CACGTTTATT
    CCACACTGTA GTTTTATGGG GATGGTGATC TCTCATGACA TGCTGCTGGG CCGCTGGCGG
    CTTTCTCTGG AGCTGTTTGG CCGTGTTTTC ATGGAGGACG TTGGAGCAGA ACCAGGATCT
    ATCCTGACAG AGCTGGGGGG TTTTGAGGTA AAGGAGTCCA AGTTTCGTCG TGAGATGGAG
    AAGCTGCGTA ACCTTCAGTC GCGTGATCTG GCCCTGGAGG TGGACCGAGA CCGTGAGCAG
    CTCATCCAGC AGACCATGCG GCAGCTGAAC GCTCACTTCG GCCGCCGCTG TACCACCACA
    CCCATGGCCG TGCACCGCGT AAAGGTGACA TTCAAGGATG AGCCTGGAGA GGGCAGTGGT
    GTCGCTCGCA GCTTCTACAC CGCCATCGCT CAGGCCTTCT TGTCCAACGA CAAGCTGCCC
    AATCTGGACT GCGTGCAGAG CGTCAGCAAG GGGATGCAGG CTAGCAATCT TATGCAGCGG
    CTGAGGAACC GAGATCGTGA GCGTGAGAGG AGGAGCGGAG GACTGAGAGC TGCATCCAGA
    CGAGATCGTG ACAGGGACTC GCGGAGGCAG TTGTCCATCG ACACCCGGCC GTTCAGACCT
    GCATCAGAAG GAAACCCCAG TGATGAACCA GAACCGCTGC CGGCTCACAG ACAGGCTCTG
    GGAGAGAGAC TTTACCCACG AGTTCATGCC ATGCAACCGG CGTTTGCTAG TAAAATCACA
    GGGATGCTGC TGGAACTGTC TCCCGCTCAG CTGCTGCTAC TCCTGGCCAG CGAAGACTCG
    CTCAGAGCCC GAGTGGAGGA GGCCATGGAG CTGCTCATAA CTCATGGACG GGAAAATGGT
    GCTGACAGTA TACTAGACCT CGGCCTCCTC GACACTCCTG AGAAAGCACA ACAGGAGAAC
    CGAAAGAGGC ATGGTTCGAC TCGCAGTGTG GTGGACATGG AGCTGGATGA CCCTGATGAT
    GGGGATGATA ACGCTCCTCT GTTTTACCAG CCTGGAAAGA GGGGTTTCTA CTCACCACGT
    CCCGGCAAGA ACACTGAGGC CAGGCTCAAC TGTTTCCGTA ACATCGGCAG GATACTGGGT
    TTGTGTCTGC TACAGAATGA GCTTTGCCCA ATCACTCTCA ACAGACACGT CATCAAGGTG
    CTGCTCGGCA GAAAGGTGAA CTGGCACGAC TTTGCGTTCT TCGACCCGGT AATGTACGAG
    AGTTTGCGGC AGCTCATCCG TCACTCTCAG ACTGAAGAGG CGGAGGCTGT GTTTGCGGCG
    ATGGACCTGG CCTTCGCTAT CGACCTCTGC AAGGAGGAGG GAGCAGGACA GGTGGAGCTG
    CTGTCCGGTG GAGTCAACAT GCCGGTGACG CCTCTGAATG TGTACGAGTA CGTGAGGAGA
    TACGCAGAGC ACCGGATGCT GGTGGTGGCA GAACAGCCTC TGCATGCCAT GCGGAAAGGT
    CTTCTGGATG TGCTTCCTAA GAACGCTCTG GAAGATCTGA CAGCGGAGGA TTTCAGACTG
    CTGGTCAACG GCTGTGGAGA AGTCAACGTC CAGATGCTCA TTAGTTTCAC TTCATTCAAT
    GATGAGTCAG GTGAGAATGC AGAGAAGCTG CTGCAGTTCA AGCGCTGGTT TTGGTCCATC
    GTTGAGAAGA TGAGCATGAC GGAAAGGCAG GACCTGGTGT ACTTCTGGAC GTCCAGCCCG
    TCTCTGCCGG CCAGTGAAGA AGGTTTCCAG CCGATGCCCT CCATCACCAT CCGCCCGCCG
    GACGACCAGC ACCTGCCCAC AGCCAACACC TGCATCTCTC GCCTCTACGT GCCACTTTAC
    TCTTCCAAAC AGATCCTCAA ACAGAAACTT TTACTAGCCA TCAAGACCAA GAACTTCGGT
    TTTGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.