hyd cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	hyd
Entrez Gene ID	41181
Full Name	hyperplastic discs
Synonyms	CG9484,Dmel\CG9484,HYD,Hyd,NP_524296.2,UBE 3A,c43,l(3)85Ea,l(3)C43,l(3)C43-3,l(3)SG33,l(3)c43,l[[3]]c43
General protein information	Preferred Names hyperplastic discs Names CG9484 gene product from transcript CG9484-RA CG9484-PA CG9484-PB hyd-PA hyd-PB
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 85E5-85E5\|3-49.0 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001300333.1	NP_001287262.1	hyperplastic discs, isoform B
NM_079572.4	NP_524296.2	hyperplastic discs, isoform A

Pathways from BioSystems

Homologies

	UBR5	XP_001100326.1
Mus musculus (house mouse)	Ubr5	NP_001074828.2
Pan troglodytes (chimpanzee)	UBR5	XP_003311934.1
Bos taurus (cattle)	UBR5	NP_001179346.1
Drosophila melanogaster (fruit fly)	hyd	NP_524296.2
Canis lupus familiaris (dog)	UBR5	XP_532290.2
Danio rerio (zebrafish)	ubr5	NP_001157866.1
Xenopus tropicalis (tropical clawed frog)	ubr5	XP_004915164.1
Homo sapiens (human)	UBR5	NP_056986.2
Gallus gallus (chicken)	UBR5	XP_003640895.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001780	XP_321295.5

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Wnt-dependent inactivation of the Groucho/TLE co-repressor by the HECT E3 ubiquitin ligase Hyd/UBR5 is a key prerequisite that enables Armadillo/beta-catenin to activate transcription.
Title: Wnt-Dependent Inactivation of the Groucho/TLE Co-repressor by the HECT E3?Ubiquitin Ligase Hyd/UBR5.

Drosophila-based findings highlight an apical role for Hyd and Sgg in initiating Hedgehog signalling, which could also be evolutionarily conserved in mammals
Title: Regulation of hedgehog Ligand Expression by the N-End Rule Ubiquitin-Protein Ligase Hyperplastic Discs and the Drosophila GSK3? Homologue, Shaggy.

hyperplastic discs (hyd), an E3 ubiquitin ligase, can differentially regulate the transcriptional outputs of Hh signaling. We show that loss of Hyd activity caused upregulation of some, but not all of Hh target genes.
Title: Hyperplastic discs differentially regulates the transcriptional outputs of hedgehog signaling.

data document that, in addition to the tumour suppressor Merlin, another tumour suppressor, Hyd, also has a function in spermatogenesis
Title: The role of Drosophila hyperplastic discs gene in spermatogenesis.

hyperplastic discs encodes a HECT domain E3 ubiquitin ligase that is likely to act by targeting Cubitus interruptus and an unknown activator of hedgehog expression for proteolysis
Title: The ubiquitin ligase Hyperplastic discs negatively regulates hedgehog and decapentaplegic expression by independent mechanisms.

The following hyd gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hyd cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa04376	NM_079572.4 Latest version!	Drosophila melanogaster hyperplastic discs (hyd), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OFa22074	NM_001300333.1 Latest version!	Drosophila melanogaster hyperplastic discs (hyd), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hyd ( NM_079572.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_524296.2 Drosophila melanogaster hyperplastic discs (hyd), transcript variant A, mRNA.

Price & Availability↑

CloneID	OFa04376
Clone ID Related Accession (Same CDS sequence)	NM_079572.4
Accession Version	NM_079572.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hyperplastic discs, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jul 15, 2014 this sequence version replaced NM_079572.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTCCA TGCAATTTGT TTTGCAACCG CTACCGGGCT CGGACGATCA GTTTATTGAA 
AGGATTCGTG AAGTGTCGGA TAAGGTGAAC CGATTTGGTT ATGGCAGCCA TCGCATATTC 
GAACAGTTGA AAATTCCAGT GAAGGAAGTC GTCATTGGTC CCGCGCACAT TGGAGTTTTA 
CTGGAGGATG GCAAGGCATT CCGCGTGTCC TTTTCTATAA ATTCTGAGAA ACTTGACTTG 
ACCAAATCAG ACGCAAAATG TTCCACCAGT GGTGGCAGCG GAACGGCCAG TGCTTCGAAA 
GCCCCATCCT CATCCCGACC GATGGCCCGC TCAAGAGCAC GCCTGCTGCG CGCAACAGGT 
CGCTCCAATT CCACCGGACA GGGGTCCGGA TCTCGTAGCA CAGGGGTCAT CATTGGAGGC 
AGTACATCGA GCCGACCCTT AGTAACAGTT CCGGCTACAT ATGTGCCGGA GGAGCTCATA 
TCCCAAGCAG AGGTGGTTCT ACAGGGCAAG AGCAGAAATC TCATAATTAG AGAATTACAG 
CGCACCAACC TCGATGTTAA CTTAGCTGTG AACAATTTGC TTTCGCGCGA TGATGAAGAA 
GCTGAAGATA CCGAAGAAGG TGCCGATAAT TACGTGCCCG AAGACCTTAT CTCCCTGCTG 
GATAATGGGT TCAGTGGAGA TAACAACAGC GTCATCATAG ATCCCTCAGA TGGGCTTTTC 
TCGGAGGAGA TATTTAGCAA CTACTCCAGC ATTCGAAACT TGCTGTTCGA TCGCATTCGT 
AGTGAACGCA GTAATGCCAA CGCAAATGCC GCAGACAGTA ACCAATCGAC GACCCGCTCA 
ACAAGTTCAG GCACTGCCTT AACTGGTAAC AGTGGACTTT CAGCTCAAAT ATCAGTTAAT 
GCTGACCGCG AGGCTTTTAG TCGTTGGCGG GATCGTCAGT ACTACGGACC ACGACGCTGG 
ATAAGCAAAG ACGACTATAC CTGGGAGAAG GATGCTGATT CCAAGAAAAA AGAGCCATCC 
CCCATGTTGT CCCCCATTTG GATATCTGAA GAATTGCAGC CATGGCCAGA GAAAAGTTCC 
GTAAGGTTCA AGACCATAGG AGCTTTGTAT TCGGAGTTCA TTGCCTTGTC AGAGAGCGGA 
GATTTGTACC AGTGGCGCTG GTCAGATGCA GAGCCTTATA AGTCGGAGAC TGAAAACGTT 
TATCATCCCA AAACTGTTTC CCTCAACATA GTTGAAAGAG TGGAGCTTAT TTCGGCCAAC 
TTCATCAGAT GCTCCGTTGT TACAGAAACC AATCGAGTAG CAACTTGGAT GGACGAGCAA 
CTAGGTTACA TCGGCGCCAA GCTGGAGCAC AGTTGCTGCG CGTTTAACGA GTTTATATCG 
GACTCCATAA CAAAGATCTA CGTCTGCTCG CTATACACGG TTGTTAAGAC CGAAAGCAAC 
AACATATATT GGTGGGGAGT CCTGCCGTTT GATCAACGCC GCTTCTTGTG GGACAAATTC 
CGGACCAAGA CCAAGAAACC CTTTAAGGTG GTTGCCACCG ATATTAATGT GGGTGCTCAA 
GTGATAATGA AGAAGTGTCC CATGTATCAA TCTGGATCAA TCGGATTCAC ATGCTCCAAT 
GGCGTCCCAA AGGTGGGGCA GCTTTTGAAT TCAGTCTGGA CCTTCACCGA TGTGTGCCGC 
ATGAAAATCA TAAATATTAA CACGAACTCT GGCGTAGACA AATCCCAAGC AGCCGGCAAT 
AATCTTAATG CCCATGGAAT TACTCCGGAT AAAGATCTGC CAAAGTCCAC GGCAATGCCC 
AGCACTGGAA GTAGTAAGAA TGGACAATCC TTCTCAAACA GCAAGGAGTC AACAGATCGC 
ATCGACATGC CACCTCCGCC TTCACCGGCA TCCAGCACAT GCAGCGACAC CGGCAGCGTC 
ACATCCCACA AGCGTACTAA GCGAGCTACT ACGAAAGAGG ACTCCAATGC CCCGCAGGAG 
GGAAGGAAGG ATGAGGAGCT GTGGGAAGTG AAGGACGTGG TGTTTGTCGA GGACAAGGTG 
GGCCCAGTGG GAAAGGTGCT TAAGGTCGAC GGCGACTTTG TTGCCGTTCG TTTTCCGGCA 
ATAAATGCAG CAGCCGTTGC TGCTGCTGCT GCCGCAACAA GTTCCACATC TAACACAGCC 
TCCACATCCA AGGAAGAGGG CAAGGAGGAC GACTGGCAGC AGTGTCGGCT GCTGAGACGC 
GAAGATGTCC AGATATTTCG CACTGCTATG TCCACCCGTG GCCCAGACTG GTTGCAAAAA 
CAGCCGAAAA AGATTAACGT TGGTGGTGAT GCAGCTGGCG CACAAATACT CACCCTGGCC 
GTGGACTCGC GCGGCATTCA TGTGATCAAG AAGGTTCTCG GCAAAATCCA CTACAGCCTT 
TACAACCTGT ATAACTGCAA GCAGGAGCAA AATTGCCTAT TTCCCACGGA CTGCAACTCT 
TTTATTGGCT CCACGCCTGG CAACATATTA ATGGCTTGCA ACGACGACTG CAGCGGAAAC 
AGCAGTACCA TTGTGCTTCG TGATGGCAAT GGAGCCCTAT ATCCCTTGGC CAAGGATTGC 
CTTGGCTCGA TCAAGGATCC GCAATGGTTT GATCTTCCTC CGGTGAAGTC AATCACTATG 
TCCACAATCT CCCTGCCGGC CATGCTCTCC GGCGTGAATT TGAAGTCAAA GGTATGCATG 
ACTGCTTTGC TCTTTGATAC TCAAAAGCTT ATGCCGCACA TCTTGCGGTG CGATGTCAAG 
AATAGCTTTG CCGCCCTCGG TCGCCTGGAG AGGGAGGATC AAGCGGATAC GGCTCTTGTC 
GTAGAAGAAC GTTGCGATGG AGCCCGAAAC ATATTCCACG CTTGCGTAAT TATGTGCGCC 
CCATCCTCAA ACAAGGATTC CCCACCAGAC TCTCCCAGCG GTGGTGTCGA GAAGAAATCA 
CTAGTGGGAC TCTCCGTGGC GCGTTCCATT CCAACTGTCT CGACAAGCGC TTACGTTAGC 
AGCATCGCAT TTGGAGCAAG TGCGTCTTCG AGCAATGAAA ACTCCAGCTT TGCAACTATG 
AGTTCATCCG CTGCCGGCTC CGCCTCATCG ACGAGCAGGG ATAATCGTAC CAATCTGCGC 
GACATGATGA ATCGTCTAAT AAATAGCGAT CAGGCTGAAC AGTCTGGCAG CCAGCCAATG 
GCTACAAACA ACGAAGACCA TGCCTACATC CCCTGGCCAG CTGAAACACC GGCGGCTAGT 
AATCTGAGCG CCAGCTCTTC GCAGAATGTC TCAGACAGCA TTGAAGATGA TATCTCAAAG 
ATAATTCCGT CCTCCTCCCA GAGTTCCATG CTCTCCAACA TCAAGTTGGG GTCCCCTACC 
TACACCTTCG ACCTGGCTCA GCGGCGGGAG CATGCTTTGA CAATCCTTCA GCAGATGTGC 
GTAAGTCCAG CCCTTCGTCC GTACCTCTGC CATATGCTGA GCACTAAGGA TGCGCAGGGC 
CAAACGCCGT TTATGCTATC CGTTTCGTGT CGCGCCTACG AGGCAGGAAT CATACTACTA 
AACACGATTC TCATGCTGTC CGAGCAGGAT CCGCAGCTAA AGGAGGCCAT GATTTTCCCG 
AATGGTTCAC CTGCGGATCA GTCACCTCTG CATGTCATCT GCTACAATGA TACCTGCTCG 
TTCACATGGA CAGGAGCCGA TCACATCAAC CAGAACATTT TCGAGTGCAA GACATGCGGC 
CTCACTGGAT CCCTGTGCTG CTGCACGGAA TGCGCGAGAG TCTGCCACAA AGGACACGAC 
TGCAAGTTGA AGCGTACGGC GCCGACTGCT TACTGTGATT GTTGGGAGAA ATGCAAGTGT 
AAGGCTCTGA TCGCTGGAAA TCTCACAAAG CGCTTCGCAC TGCTGTGCAA ACTAGTATCC 
TGCACAGATT TGGTCACCAA ATTTAACTCC AAGGGTGAAT CGATTCTTCT TTTCCTTATT 
CAAACGGTTG GACGCCAGAT TGTAGAGCAG CGACAGTACC GCTTTAGTGT AAGGGTTCGC 
AATGTTAGCA CCGCTGCCAC CGGAGCGACC GGAAACAATT CAGTGATATC AAATCGGAAG 
ACCTCCGCAG CCGAAATTGA TAATGATATG CCGGACCATG ATCTGGAGCC ACCGAAGTTC 
GCCAGAAAGG CTTTGGAGAG ACTACTGATC GACTGGAACG CCGTGCGCTC AATGATCATG 
AGCGGTGCGG AGAGAGGCGA TGTACCGAAT CCAGCAGGCA GTGCATCGGA GAACTCTAAT 
TCGGAAGGCT TTAATATGTT TATCCAGACG CAGCATGGCT CTACGCTTCT CGACAAGTTT 
ACCCACAGTT TGATCGTAAA GTGCACTAGC GATCATTTGG ACACTTTGCT ACTCACCCTG 
GTGCGCGAAT TGCAGAACGC AAGCGTGTCC AATCGTAGCA AGGAAGCTGA GGAAGTAGTG 
CGTCGCTTCG TGCGTTCCGT AGCACGCGTT TTTGTCATTT TCAACCTGGA AAAGCAGCCT 
AATCCGGAGA AGCGAAAGTC GCACTCATCC TGCAACAAAT ACGTGCAGAG CTGTGTTAAA 
GTATTCCAAA CGCTGCACAA GATATCCATC GAGGAGTTAT GTGAGGTATC CGAGGCTCTG 
ATCGCTCCAG TCCGACTGGG CGTTGTGCGT CCAACAGCTC CGTTTACGAT GTCATCGTCG 
AATCTGGATA ACTCTGACGA TTTGTTCAGC GTTGATCCTT TAGCCCCCTC TAACGTGGAG 
TCTCCTTCTG AGCAAATACT TGTCCACGAT GCTGGAAACG ATCAGAGCGC CAATTTTAAT 
ATTCAACAAA ACTACGATGT AGTTGCTATG GAAACTATAC GCGACGCCTC CGAATCGGAA 
GAAGTTATAA ACCGGGAGGC TAATTCCCAC AATCAAGACG ATGAGCTAAT TGAAAATCAA 
CGAAATGAGG ACGGCATGCA AGATGACGAG AGCGATAATG ACTTTACTTT CAATGACGCC 
GAGACTGAGT CCGACTCCGA TGACAACCAA AGCAATCAAG AGGTGCAGAG GAGCGTGCAA 
GCAGGAGCAA CCGTTGGCTC AGAAAATGAC ATTGGTGTTC TCTTCCTAGA GGATGAATCG 
GGAGACTCCT CTGCCCAAGA AGAAGATGGA TCGGAGGATG GCGAGTCCGA CGATCAGTCC 
GACGAGTTCA ATTTCAATGA GCAGCAACTT GAACGCCGCT CTACCAACTC GAACGCTCGC 
AGCGATCTGG CTCCTCAAAC GATGCAATGG GCCATACGCA GTCGTGACAC TGCGCGCTCC 
TCCGTGAGGG TGCCCACCGG GTCTAATATG GTTTTTATTG ACCCCATGGC TCTGCGCCGC 
TCCACTGTAC CCGCCAGCAC TACAGTCACT ACCCCATCGA TTGAACCACA CACCATGGCG 
ACAACGGCCA GCAACTTGGC ACGGGCTTTC GGAATCACAA TAAGGCAAAT ATCCGAGCTT 
ATAAGCATTT TATCCTATAA CGTACTAAAT GACATCGAAA CATCATTAAA AATTCAAAAT 
GATGAAGCAA TCGCAGTCCA AGCATTTGTG GAAAAGCGTT TAAAGGCTAC GTGGGATTGG 
ATGTTTACGG TAATGGACGG TACCGAAGCA CAGCTAAAGT TCGGAGCTTA TTTGACCAAC 
TACACGGATC CCAACCATCC GCTGCATCCG CTCAACCTAA GTGCCCAGGC TTCGAGTAGC 
CAGACTCCGG CTCCTGCCAC ATCGTCGTCA GTTAATGGTG TAAATATTAT GGGATCCAAT 
TCACGCCGCG ACTTCTTCAC CTACTGTTTA TCCCTGATGC GCTCACACAC CTCTGAGCAC 
CGAGACGCCT TGCCAGTGTT GGATATAACA GCCTTGCGGC ACATTGCCTA TGTACTCGAT 
GCCTTTGTCT ACTATATGCG CAATGATTCA GGCTTCTATG ATAAGCAAGA TACCATTTCT 
GGCCGTATAA ACAATTTGTC ACCTATGACT GAGAGCTATG ATACGGACGA CGAGTTGGCT 
AATCTAGAGG AGTTTAACGC CGATGTTCAG ATGTCCGCTA GCAGCATGCC TTCAGGAAGC 
CAGGGCACAC GCCGCCATGC ATTCTTTGCT CGATCTGAAT CGACATTAAG CTTGGGCTGC 
TCAGCGCCAG AAGGCTTTGA ATTGCCACTG GATATGGCCA TGCCTCTCGC CGATAAGCCG 
CACTTGCTAC AACCAAATTC TAAGCGTCAG GAACTCTTCG CAAACCTACC GCTTCTCGTG 
ACCACGAATG CCAACAACAG CGGTGCCACT AATGATGGTG ATGGTGGCAG CATTTTTGAT 
TATACTCCAA CTAGACTGGG ATTCTCGAAT TCCTTGAAAA GGAACGAGCG TGTTTACGAA 
ACAGTCCCGA TCGATTCTAG TAAGACGGGT GATGGAAATG TAACTAATAA GGCTGAGGGC 
TCAACAGACT CTAATATTTA CGTACAATTA AAGAAGAAAC AGGGCTCGGA TGATTTCAAA 
TCCCACAAAG AGGCTGACGG CAATCAAAGC AAGTACGAAA AAGTCGTATT AATGGAAACT 
GATGACAGTC TTCCAAGTAC AAGTAAATCG ACTGAAGCAT TAATGGCCAC ACGACCAGAG 
GTTATCATAG CGCCAAATAA AGCCTCAGTG TCGCCAGCGA CTGCCGCTCG CAGCGTCATA 
GTTCTCGCAG GTGGTTCCTG TTTAAAGACA ATTGATTCCG ACATAAACAA CTACTCGGCT 
TCAAATCTAT CGACCGCAGA ACAAGCCAAA TGTGATACAC AATATCAAAA GTCAACATCA 
GACCACCTGT TATTATTTCC TGCTCGTGGT TCTCAGTTTT ACCAAAGTAA TTTTTCGGAA 
TTGCCGTCGT GGAACTTTTT ACTTAGCCGC TGGAAGCTAA CTCTGGACTT ATTCGGTCGC 
GTTTTTATGG ACGACGTCGG AATGGAACAC GGCTCTGTGC TGCCGGAACT GCGAGGCTTT 
CCCGTGAAGG AGATGCGTTT CCGTCGCCAC ATGGAAAAAT TGCGCAACGG ACAACAACGT 
GATTTGGTGC TTTGCAAGTT GGAGCGCAAT CGTGAAAGCT TAATTGTTCA AACGTTCAAG 
GAACTGAATA CCCAGTTTGG CAACCAAAGT CGGCGCATCC AGCCGCCCAT CACATTCAAC 
AGAGTAAAGG TGACATTCAA AGACGAGCCG GGCGAGGGAT CGGGTGTAGC GCGCAGTTTT 
TACACATCGA TCGCAGAAGC ATTGCTGGCC AGTGCTAAGA TCCCCAACTT GGAGTCCGTA 
CAGGTGGGCA CCAATCATAG CAAGTACGTA GTGCCGTTCT CGAGCATCCT GCGCAGCAGA 
ACAGTATCGG GGTCTAGTCG CGATCAGTCC ACTCTGCAGA GGCGTGGTTC CAACAGCAAA 
ATATTGTGGC GTTCGGCCCG TGAGAGGAAG GCATTGAATT TGGATGCCAG ACCGTATACT 
CCACCAAATA GCTCAGATAA TGCTACGCCG GAAAGCCTGA ATGATCACTT GTCCGTTCAC 
CTACAACAAA TTGGGGAACG TCTTTACCCG AAGATCCATT CGATAAACCA AACGCATGCA 
CCGAAGATAA CAGGAATGCT GCTGGAGATA CCCACTCCGC AGCTCCTGTC CGTAATTTCC 
TCAGATGAGA CGCTCCGTCA GAAAGTGAAT GAAGCGATAG AAATAATCAC GTTTAAACAA 
AAATCTGAGA CCAGTGCACA AAGTAGTCAA CCAAAAAAAT CACCATCTGT CGTGGTAGTG 
GATCCCGTTG ATGATGATAA TGAACCATTA TTTTACTCAC CGGGAAAGCG GGGCTTTTAC 
ACACCACGCC AAGGCTTTGC ATCATTCGAG CGCATTAATG CATTTAGAAA TATTGGAAGA 
CTTATTGGAC TTTGTCTACT GCAAAACGAA TTGCTTCCAC TGTTCCTACA AAGACATGTA 
CTAAAATACA TTCTTGGGCG TAAAATTAAG TTCCACGACT TGGCATTTTT CGATCCAGCA 
TTGTACGAAT CTTTTAGACA AATTATTCAA AATGCCCAAA CAAAGGAAGG CGAGGAAACT 
ATAAATCGTA TGGAGCTATG CTTTGTTATC GATTTGATGA AGGAGGAGGG CTGTGGTAAC 
AGAGAGCTTA TCCCAGGAGG GCGCGATGTC GCTGTCACAT CGAGTAATAT ATTCGAGTAC 
GTCAGACGCT ATACAGAATA TAGACTAATC AAATCCCAAG AAAAGGCACT GGAGGCACTG 
AAGGACGGAG TTTTTGACGT TTTGCCCGAT AACAGCATGA TTAACTTAAC CGCTGAGGAC 
TTGCGTCTGC TGCTAAACGG TGTTGGTGAT ATAAATGTTT CGACACTAAT TTCATACACT 
ACCTTTAACG ACGAATCTAG CGAGGGTCCT GATAAACTTT TAAAATTTAA GAAATGGTTT 
TGGAGTATTG TGGAGAAAAT GAACATTATG GAACGCCAGG ATTTGGTTTA CTTCTGGACC 
GGCTCTCCTG CGCTTCCAGC TTCGGAGGAG GGATTCCAAC CATTGCCATC TGTCACCATA 
AGACCTGCGG ATGACTCCCA TCTACCGACT GCGAATACTT GTATCTCTCG GCTGTATATC 
CCTCTTTACT CCAGCAAATC TATTTTACGC AGTAAAATGC TGATGGCCAT TAAATCCAAA 
AATTTTGGAT TTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_524296.2
CDS	91..8748
Translation	MVSMQFVLQPLPGSDDQFIERIREVSDKVNRFGYGSHRIFEQLKIPVKEVVIGPAHIGVLLEDGKAFRVSFSINSEKLDLTKSDAKCSTSGGSGTASASKAPSSSRPMARSRARLLRATGRSNSTGQGSGSRSTGVIIGGSTSSRPLVTVPATYVPEELISQAEVVLQGKSRNLIIRELQRTNLDVNLAVNNLLSRDDEEAEDTEEGADNYVPEDLISLLDNGFSGDNNSVIIDPSDGLFSEEIFSNYSSIRNLLFDRIRSERSNANANAADSNQSTTRSTSSGTALTGNSGLSAQISVNADREAFSRWRDRQYYGPRRWISKDDYTWEKDADSKKKEPSPMLSPIWISEELQPWPEKSSVRFKTIGALYSEFIALSESGDLYQWRWSDAEPYKSETENVYHPKTVSLNIVERVELISANFIRCSVVTETNRVATWMDEQLGYIGAKLEHSCCAFNEFISDSITKIYVCSLYTVVKTESNNIYWWGVLPFDQRRFLWDKFRTKTKKPFKVVATDINVGAQVIMKKCPMYQSGSIGFTCSNGVPKVGQLLNSVWTFTDVCRMKIININTNSGVDKSQAAGNNLNAHGITPDKDLPKSTAMPSTGSSKNGQSFSNSKESTDRIDMPPPPSPASSTCSDTGSVTSHKRTKRATTKEDSNAPQEGRKDEELWEVKDVVFVEDKVGPVGKVLKVDGDFVAVRFPAINAAAVAAAAAATSSTSNTASTSKEEGKEDDWQQCRLLRREDVQIFRTAMSTRGPDWLQKQPKKINVGGDAAGAQILTLAVDSRGIHVIKKVLGKIHYSLYNLYNCKQEQNCLFPTDCNSFIGSTPGNILMACNDDCSGNSSTIVLRDGNGALYPLAKDCLGSIKDPQWFDLPPVKSITMSTISLPAMLSGVNLKSKVCMTALLFDTQKLMPHILRCDVKNSFAALGRLEREDQADTALVVEERCDGARNIFHACVIMCAPSSNKDSPPDSPSGGVEKKSLVGLSVARSIPTVSTSAYVSSIAFGASASSSNENSSFATMSSSAAGSASSTSRDNRTNLRDMMNRLINSDQAEQSGSQPMATNNEDHAYIPWPAETPAASNLSASSSQNVSDSIEDDISKIIPSSSQSSMLSNIKLGSPTYTFDLAQRREHALTILQQMCVSPALRPYLCHMLSTKDAQGQTPFMLSVSCRAYEAGIILLNTILMLSEQDPQLKEAMIFPNGSPADQSPLHVICYNDTCSFTWTGADHINQNIFECKTCGLTGSLCCCTECARVCHKGHDCKLKRTAPTAYCDCWEKCKCKALIAGNLTKRFALLCKLVSCTDLVTKFNSKGESILLFLIQTVGRQIVEQRQYRFSVRVRNVSTAATGATGNNSVISNRKTSAAEIDNDMPDHDLEPPKFARKALERLLIDWNAVRSMIMSGAERGDVPNPAGSASENSNSEGFNMFIQTQHGSTLLDKFTHSLIVKCTSDHLDTLLLTLVRELQNASVSNRSKEAEEVVRRFVRSVARVFVIFNLEKQPNPEKRKSHSSCNKYVQSCVKVFQTLHKISIEELCEVSEALIAPVRLGVVRPTAPFTMSSSNLDNSDDLFSVDPLAPSNVESPSEQILVHDAGNDQSANFNIQQNYDVVAMETIRDASESEEVINREANSHNQDDELIENQRNEDGMQDDESDNDFTFNDAETESDSDDNQSNQEVQRSVQAGATVGSENDIGVLFLEDESGDSSAQEEDGSEDGESDDQSDEFNFNEQQLERRSTNSNARSDLAPQTMQWAIRSRDTARSSVRVPTGSNMVFIDPMALRRSTVPASTTVTTPSIEPHTMATTASNLARAFGITIRQISELISILSYNVLNDIETSLKIQNDEAIAVQAFVEKRLKATWDWMFTVMDGTEAQLKFGAYLTNYTDPNHPLHPLNLSAQASSSQTPAPATSSSVNGVNIMGSNSRRDFFTYCLSLMRSHTSEHRDALPVLDITALRHIAYVLDAFVYYMRNDSGFYDKQDTISGRINNLSPMTESYDTDDELANLEEFNADVQMSASSMPSGSQGTRRHAFFARSESTLSLGCSAPEGFELPLDMAMPLADKPHLLQPNSKRQELFANLPLLVTTNANNSGATNDGDGGSIFDYTPTRLGFSNSLKRNERVYETVPIDSSKTGDGNVTNKAEGSTDSNIYVQLKKKQGSDDFKSHKEADGNQSKYEKVVLMETDDSLPSTSKSTEALMATRPEVIIAPNKASVSPATAARSVIVLAGGSCLKTIDSDINNYSASNLSTAEQAKCDTQYQKSTSDHLLLFPARGSQFYQSNFSELPSWNFLLSRWKLTLDLFGRVFMDDVGMEHGSVLPELRGFPVKEMRFRRHMEKLRNGQQRDLVLCKLERNRESLIVQTFKELNTQFGNQSRRIQPPITFNRVKVTFKDEPGEGSGVARSFYTSIAEALLASAKIPNLESVQVGTNHSKYVVPFSSILRSRTVSGSSRDQSTLQRRGSNSKILWRSARERKALNLDARPYTPPNSSDNATPESLNDHLSVHLQQIGERLYPKIHSINQTHAPKITGMLLEIPTPQLLSVISSDETLRQKVNEAIEIITFKQKSETSAQSSQPKKSPSVVVVDPVDDDNEPLFYSPGKRGFYTPRQGFASFERINAFRNIGRLIGLCLLQNELLPLFLQRHVLKYILGRKIKFHDLAFFDPALYESFRQIIQNAQTKEGEETINRMELCFVIDLMKEEGCGNRELIPGGRDVAVTSSNIFEYVRRYTEYRLIKSQEKALEALKDGVFDVLPDNSMINLTAEDLRLLLNGVGDINVSTLISYTTFNDESSEGPDKLLKFKKWFWSIVEKMNIMERQDLVYFWTGSPALPASEEGFQPLPSVTIRPADDSHLPTANTCISRLYIPLYSSKSILRSKMLMAIKSKNFGFV

Target ORF information:

RefSeq Version	NM_079572.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hyperplastic discs (hyd), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_079572.4

ORF Insert Sequence:

ATGGTTTCCA TGCAATTTGT TTTGCAACCG CTACCGGGCT CGGACGATCA GTTTATTGAA 
AGGATTCGTG AAGTGTCGGA TAAGGTGAAC CGATTTGGTT ATGGCAGCCA TCGCATATTC 
GAACAGTTGA AAATTCCAGT GAAGGAAGTC GTCATTGGTC CCGCGCACAT TGGAGTTTTA 
CTGGAGGATG GCAAGGCATT CCGCGTGTCC TTTTCTATAA ATTCTGAGAA ACTTGACTTG 
ACCAAATCAG ACGCAAAATG TTCCACCAGT GGTGGCAGCG GAACGGCCAG TGCTTCGAAA 
GCCCCATCCT CATCCCGACC GATGGCCCGC TCAAGAGCAC GCCTGCTGCG CGCAACAGGT 
CGCTCCAATT CCACCGGACA GGGGTCCGGA TCTCGTAGCA CAGGGGTCAT CATTGGAGGC 
AGTACATCGA GCCGACCCTT AGTAACAGTT CCGGCTACAT ATGTGCCGGA GGAGCTCATA 
TCCCAAGCAG AGGTGGTTCT ACAGGGCAAG AGCAGAAATC TCATAATTAG AGAATTACAG 
CGCACCAACC TCGATGTTAA CTTAGCTGTG AACAATTTGC TTTCGCGCGA TGATGAAGAA 
GCTGAAGATA CCGAAGAAGG TGCCGATAAT TACGTGCCCG AAGACCTTAT CTCCCTGCTG 
GATAATGGGT TCAGTGGAGA TAACAACAGC GTCATCATAG ATCCCTCAGA TGGGCTTTTC 
TCGGAGGAGA TATTTAGCAA CTACTCCAGC ATTCGAAACT TGCTGTTCGA TCGCATTCGT 
AGTGAACGCA GTAATGCCAA CGCAAATGCC GCAGACAGTA ACCAATCGAC GACCCGCTCA 
ACAAGTTCAG GCACTGCCTT AACTGGTAAC AGTGGACTTT CAGCTCAAAT ATCAGTTAAT 
GCTGACCGCG AGGCTTTTAG TCGTTGGCGG GATCGTCAGT ACTACGGACC ACGACGCTGG 
ATAAGCAAAG ACGACTATAC CTGGGAGAAG GATGCTGATT CCAAGAAAAA AGAGCCATCC 
CCCATGTTGT CCCCCATTTG GATATCTGAA GAATTGCAGC CATGGCCAGA GAAAAGTTCC 
GTAAGGTTCA AGACCATAGG AGCTTTGTAT TCGGAGTTCA TTGCCTTGTC AGAGAGCGGA 
GATTTGTACC AGTGGCGCTG GTCAGATGCA GAGCCTTATA AGTCGGAGAC TGAAAACGTT 
TATCATCCCA AAACTGTTTC CCTCAACATA GTTGAAAGAG TGGAGCTTAT TTCGGCCAAC 
TTCATCAGAT GCTCCGTTGT TACAGAAACC AATCGAGTAG CAACTTGGAT GGACGAGCAA 
CTAGGTTACA TCGGCGCCAA GCTGGAGCAC AGTTGCTGCG CGTTTAACGA GTTTATATCG 
GACTCCATAA CAAAGATCTA CGTCTGCTCG CTATACACGG TTGTTAAGAC CGAAAGCAAC 
AACATATATT GGTGGGGAGT CCTGCCGTTT GATCAACGCC GCTTCTTGTG GGACAAATTC 
CGGACCAAGA CCAAGAAACC CTTTAAGGTG GTTGCCACCG ATATTAATGT GGGTGCTCAA 
GTGATAATGA AGAAGTGTCC CATGTATCAA TCTGGATCAA TCGGATTCAC ATGCTCCAAT 
GGCGTCCCAA AGGTGGGGCA GCTTTTGAAT TCAGTCTGGA CCTTCACCGA TGTGTGCCGC 
ATGAAAATCA TAAATATTAA CACGAACTCT GGCGTAGACA AATCCCAAGC AGCCGGCAAT 
AATCTTAATG CCCATGGAAT TACTCCGGAT AAAGATCTGC CAAAGTCCAC GGCAATGCCC 
AGCACTGGAA GTAGTAAGAA TGGACAATCC TTCTCAAACA GCAAGGAGTC AACAGATCGC 
ATCGACATGC CACCTCCGCC TTCACCGGCA TCCAGCACAT GCAGCGACAC CGGCAGCGTC 
ACATCCCACA AGCGTACTAA GCGAGCTACT ACGAAAGAGG ACTCCAATGC CCCGCAGGAG 
GGAAGGAAGG ATGAGGAGCT GTGGGAAGTG AAGGACGTGG TGTTTGTCGA GGACAAGGTG 
GGCCCAGTGG GAAAGGTGCT TAAGGTCGAC GGCGACTTTG TTGCCGTTCG TTTTCCGGCA 
ATAAATGCAG CAGCCGTTGC TGCTGCTGCT GCCGCAACAA GTTCCACATC TAACACAGCC 
TCCACATCCA AGGAAGAGGG CAAGGAGGAC GACTGGCAGC AGTGTCGGCT GCTGAGACGC 
GAAGATGTCC AGATATTTCG CACTGCTATG TCCACCCGTG GCCCAGACTG GTTGCAAAAA 
CAGCCGAAAA AGATTAACGT TGGTGGTGAT GCAGCTGGCG CACAAATACT CACCCTGGCC 
GTGGACTCGC GCGGCATTCA TGTGATCAAG AAGGTTCTCG GCAAAATCCA CTACAGCCTT 
TACAACCTGT ATAACTGCAA GCAGGAGCAA AATTGCCTAT TTCCCACGGA CTGCAACTCT 
TTTATTGGCT CCACGCCTGG CAACATATTA ATGGCTTGCA ACGACGACTG CAGCGGAAAC 
AGCAGTACCA TTGTGCTTCG TGATGGCAAT GGAGCCCTAT ATCCCTTGGC CAAGGATTGC 
CTTGGCTCGA TCAAGGATCC GCAATGGTTT GATCTTCCTC CGGTGAAGTC AATCACTATG 
TCCACAATCT CCCTGCCGGC CATGCTCTCC GGCGTGAATT TGAAGTCAAA GGTATGCATG 
ACTGCTTTGC TCTTTGATAC TCAAAAGCTT ATGCCGCACA TCTTGCGGTG CGATGTCAAG 
AATAGCTTTG CCGCCCTCGG TCGCCTGGAG AGGGAGGATC AAGCGGATAC GGCTCTTGTC 
GTAGAAGAAC GTTGCGATGG AGCCCGAAAC ATATTCCACG CTTGCGTAAT TATGTGCGCC 
CCATCCTCAA ACAAGGATTC CCCACCAGAC TCTCCCAGCG GTGGTGTCGA GAAGAAATCA 
CTAGTGGGAC TCTCCGTGGC GCGTTCCATT CCAACTGTCT CGACAAGCGC TTACGTTAGC 
AGCATCGCAT TTGGAGCAAG TGCGTCTTCG AGCAATGAAA ACTCCAGCTT TGCAACTATG 
AGTTCATCCG CTGCCGGCTC CGCCTCATCG ACGAGCAGGG ATAATCGTAC CAATCTGCGC 
GACATGATGA ATCGTCTAAT AAATAGCGAT CAGGCTGAAC AGTCTGGCAG CCAGCCAATG 
GCTACAAACA ACGAAGACCA TGCCTACATC CCCTGGCCAG CTGAAACACC GGCGGCTAGT 
AATCTGAGCG CCAGCTCTTC GCAGAATGTC TCAGACAGCA TTGAAGATGA TATCTCAAAG 
ATAATTCCGT CCTCCTCCCA GAGTTCCATG CTCTCCAACA TCAAGTTGGG GTCCCCTACC 
TACACCTTCG ACCTGGCTCA GCGGCGGGAG CATGCTTTGA CAATCCTTCA GCAGATGTGC 
GTAAGTCCAG CCCTTCGTCC GTACCTCTGC CATATGCTGA GCACTAAGGA TGCGCAGGGC 
CAAACGCCGT TTATGCTATC CGTTTCGTGT CGCGCCTACG AGGCAGGAAT CATACTACTA 
AACACGATTC TCATGCTGTC CGAGCAGGAT CCGCAGCTAA AGGAGGCCAT GATTTTCCCG 
AATGGTTCAC CTGCGGATCA GTCACCTCTG CATGTCATCT GCTACAATGA TACCTGCTCG 
TTCACATGGA CAGGAGCCGA TCACATCAAC CAGAACATTT TCGAGTGCAA GACATGCGGC 
CTCACTGGAT CCCTGTGCTG CTGCACGGAA TGCGCGAGAG TCTGCCACAA AGGACACGAC 
TGCAAGTTGA AGCGTACGGC GCCGACTGCT TACTGTGATT GTTGGGAGAA ATGCAAGTGT 
AAGGCTCTGA TCGCTGGAAA TCTCACAAAG CGCTTCGCAC TGCTGTGCAA ACTAGTATCC 
TGCACAGATT TGGTCACCAA ATTTAACTCC AAGGGTGAAT CGATTCTTCT TTTCCTTATT 
CAAACGGTTG GACGCCAGAT TGTAGAGCAG CGACAGTACC GCTTTAGTGT AAGGGTTCGC 
AATGTTAGCA CCGCTGCCAC CGGAGCGACC GGAAACAATT CAGTGATATC AAATCGGAAG 
ACCTCCGCAG CCGAAATTGA TAATGATATG CCGGACCATG ATCTGGAGCC ACCGAAGTTC 
GCCAGAAAGG CTTTGGAGAG ACTACTGATC GACTGGAACG CCGTGCGCTC AATGATCATG 
AGCGGTGCGG AGAGAGGCGA TGTACCGAAT CCAGCAGGCA GTGCATCGGA GAACTCTAAT 
TCGGAAGGCT TTAATATGTT TATCCAGACG CAGCATGGCT CTACGCTTCT CGACAAGTTT 
ACCCACAGTT TGATCGTAAA GTGCACTAGC GATCATTTGG ACACTTTGCT ACTCACCCTG 
GTGCGCGAAT TGCAGAACGC AAGCGTGTCC AATCGTAGCA AGGAAGCTGA GGAAGTAGTG 
CGTCGCTTCG TGCGTTCCGT AGCACGCGTT TTTGTCATTT TCAACCTGGA AAAGCAGCCT 
AATCCGGAGA AGCGAAAGTC GCACTCATCC TGCAACAAAT ACGTGCAGAG CTGTGTTAAA 
GTATTCCAAA CGCTGCACAA GATATCCATC GAGGAGTTAT GTGAGGTATC CGAGGCTCTG 
ATCGCTCCAG TCCGACTGGG CGTTGTGCGT CCAACAGCTC CGTTTACGAT GTCATCGTCG 
AATCTGGATA ACTCTGACGA TTTGTTCAGC GTTGATCCTT TAGCCCCCTC TAACGTGGAG 
TCTCCTTCTG AGCAAATACT TGTCCACGAT GCTGGAAACG ATCAGAGCGC CAATTTTAAT 
ATTCAACAAA ACTACGATGT AGTTGCTATG GAAACTATAC GCGACGCCTC CGAATCGGAA 
GAAGTTATAA ACCGGGAGGC TAATTCCCAC AATCAAGACG ATGAGCTAAT TGAAAATCAA 
CGAAATGAGG ACGGCATGCA AGATGACGAG AGCGATAATG ACTTTACTTT CAATGACGCC 
GAGACTGAGT CCGACTCCGA TGACAACCAA AGCAATCAAG AGGTGCAGAG GAGCGTGCAA 
GCAGGAGCAA CCGTTGGCTC AGAAAATGAC ATTGGTGTTC TCTTCCTAGA GGATGAATCG 
GGAGACTCCT CTGCCCAAGA AGAAGATGGA TCGGAGGATG GCGAGTCCGA CGATCAGTCC 
GACGAGTTCA ATTTCAATGA GCAGCAACTT GAACGCCGCT CTACCAACTC GAACGCTCGC 
AGCGATCTGG CTCCTCAAAC GATGCAATGG GCCATACGCA GTCGTGACAC TGCGCGCTCC 
TCCGTGAGGG TGCCCACCGG GTCTAATATG GTTTTTATTG ACCCCATGGC TCTGCGCCGC 
TCCACTGTAC CCGCCAGCAC TACAGTCACT ACCCCATCGA TTGAACCACA CACCATGGCG 
ACAACGGCCA GCAACTTGGC ACGGGCTTTC GGAATCACAA TAAGGCAAAT ATCCGAGCTT 
ATAAGCATTT TATCCTATAA CGTACTAAAT GACATCGAAA CATCATTAAA AATTCAAAAT 
GATGAAGCAA TCGCAGTCCA AGCATTTGTG GAAAAGCGTT TAAAGGCTAC GTGGGATTGG 
ATGTTTACGG TAATGGACGG TACCGAAGCA CAGCTAAAGT TCGGAGCTTA TTTGACCAAC 
TACACGGATC CCAACCATCC GCTGCATCCG CTCAACCTAA GTGCCCAGGC TTCGAGTAGC 
CAGACTCCGG CTCCTGCCAC ATCGTCGTCA GTTAATGGTG TAAATATTAT GGGATCCAAT 
TCACGCCGCG ACTTCTTCAC CTACTGTTTA TCCCTGATGC GCTCACACAC CTCTGAGCAC 
CGAGACGCCT TGCCAGTGTT GGATATAACA GCCTTGCGGC ACATTGCCTA TGTACTCGAT 
GCCTTTGTCT ACTATATGCG CAATGATTCA GGCTTCTATG ATAAGCAAGA TACCATTTCT 
GGCCGTATAA ACAATTTGTC ACCTATGACT GAGAGCTATG ATACGGACGA CGAGTTGGCT 
AATCTAGAGG AGTTTAACGC CGATGTTCAG ATGTCCGCTA GCAGCATGCC TTCAGGAAGC 
CAGGGCACAC GCCGCCATGC ATTCTTTGCT CGATCTGAAT CGACATTAAG CTTGGGCTGC 
TCAGCGCCAG AAGGCTTTGA ATTGCCACTG GATATGGCCA TGCCTCTCGC CGATAAGCCG 
CACTTGCTAC AACCAAATTC TAAGCGTCAG GAACTCTTCG CAAACCTACC GCTTCTCGTG 
ACCACGAATG CCAACAACAG CGGTGCCACT AATGATGGTG ATGGTGGCAG CATTTTTGAT 
TATACTCCAA CTAGACTGGG ATTCTCGAAT TCCTTGAAAA GGAACGAGCG TGTTTACGAA 
ACAGTCCCGA TCGATTCTAG TAAGACGGGT GATGGAAATG TAACTAATAA GGCTGAGGGC 
TCAACAGACT CTAATATTTA CGTACAATTA AAGAAGAAAC AGGGCTCGGA TGATTTCAAA 
TCCCACAAAG AGGCTGACGG CAATCAAAGC AAGTACGAAA AAGTCGTATT AATGGAAACT 
GATGACAGTC TTCCAAGTAC AAGTAAATCG ACTGAAGCAT TAATGGCCAC ACGACCAGAG 
GTTATCATAG CGCCAAATAA AGCCTCAGTG TCGCCAGCGA CTGCCGCTCG CAGCGTCATA 
GTTCTCGCAG GTGGTTCCTG TTTAAAGACA ATTGATTCCG ACATAAACAA CTACTCGGCT 
TCAAATCTAT CGACCGCAGA ACAAGCCAAA TGTGATACAC AATATCAAAA GTCAACATCA 
GACCACCTGT TATTATTTCC TGCTCGTGGT TCTCAGTTTT ACCAAAGTAA TTTTTCGGAA 
TTGCCGTCGT GGAACTTTTT ACTTAGCCGC TGGAAGCTAA CTCTGGACTT ATTCGGTCGC 
GTTTTTATGG ACGACGTCGG AATGGAACAC GGCTCTGTGC TGCCGGAACT GCGAGGCTTT 
CCCGTGAAGG AGATGCGTTT CCGTCGCCAC ATGGAAAAAT TGCGCAACGG ACAACAACGT 
GATTTGGTGC TTTGCAAGTT GGAGCGCAAT CGTGAAAGCT TAATTGTTCA AACGTTCAAG 
GAACTGAATA CCCAGTTTGG CAACCAAAGT CGGCGCATCC AGCCGCCCAT CACATTCAAC 
AGAGTAAAGG TGACATTCAA AGACGAGCCG GGCGAGGGAT CGGGTGTAGC GCGCAGTTTT 
TACACATCGA TCGCAGAAGC ATTGCTGGCC AGTGCTAAGA TCCCCAACTT GGAGTCCGTA 
CAGGTGGGCA CCAATCATAG CAAGTACGTA GTGCCGTTCT CGAGCATCCT GCGCAGCAGA 
ACAGTATCGG GGTCTAGTCG CGATCAGTCC ACTCTGCAGA GGCGTGGTTC CAACAGCAAA 
ATATTGTGGC GTTCGGCCCG TGAGAGGAAG GCATTGAATT TGGATGCCAG ACCGTATACT 
CCACCAAATA GCTCAGATAA TGCTACGCCG GAAAGCCTGA ATGATCACTT GTCCGTTCAC 
CTACAACAAA TTGGGGAACG TCTTTACCCG AAGATCCATT CGATAAACCA AACGCATGCA 
CCGAAGATAA CAGGAATGCT GCTGGAGATA CCCACTCCGC AGCTCCTGTC CGTAATTTCC 
TCAGATGAGA CGCTCCGTCA GAAAGTGAAT GAAGCGATAG AAATAATCAC GTTTAAACAA 
AAATCTGAGA CCAGTGCACA AAGTAGTCAA CCAAAAAAAT CACCATCTGT CGTGGTAGTG 
GATCCCGTTG ATGATGATAA TGAACCATTA TTTTACTCAC CGGGAAAGCG GGGCTTTTAC 
ACACCACGCC AAGGCTTTGC ATCATTCGAG CGCATTAATG CATTTAGAAA TATTGGAAGA 
CTTATTGGAC TTTGTCTACT GCAAAACGAA TTGCTTCCAC TGTTCCTACA AAGACATGTA 
CTAAAATACA TTCTTGGGCG TAAAATTAAG TTCCACGACT TGGCATTTTT CGATCCAGCA 
TTGTACGAAT CTTTTAGACA AATTATTCAA AATGCCCAAA CAAAGGAAGG CGAGGAAACT 
ATAAATCGTA TGGAGCTATG CTTTGTTATC GATTTGATGA AGGAGGAGGG CTGTGGTAAC 
AGAGAGCTTA TCCCAGGAGG GCGCGATGTC GCTGTCACAT CGAGTAATAT ATTCGAGTAC 
GTCAGACGCT ATACAGAATA TAGACTAATC AAATCCCAAG AAAAGGCACT GGAGGCACTG 
AAGGACGGAG TTTTTGACGT TTTGCCCGAT AACAGCATGA TTAACTTAAC CGCTGAGGAC 
TTGCGTCTGC TGCTAAACGG TGTTGGTGAT ATAAATGTTT CGACACTAAT TTCATACACT 
ACCTTTAACG ACGAATCTAG CGAGGGTCCT GATAAACTTT TAAAATTTAA GAAATGGTTT 
TGGAGTATTG TGGAGAAAAT GAACATTATG GAACGCCAGG ATTTGGTTTA CTTCTGGACC 
GGCTCTCCTG CGCTTCCAGC TTCGGAGGAG GGATTCCAAC CATTGCCATC TGTCACCATA 
AGACCTGCGG ATGACTCCCA TCTACCGACT GCGAATACTT GTATCTCTCG GCTGTATATC 
CCTCTTTACT CCAGCAAATC TATTTTACGC AGTAAAATGC TGATGGCCAT TAAATCCAAA 
AATTTTGGAT TTGTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hyd ( NM_001300333.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001287262.1 Drosophila melanogaster hyperplastic discs (hyd), transcript variant B, mRNA.

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CloneID	OFa22074
Clone ID Related Accession (Same CDS sequence)	NM_001300333.1
Accession Version	NM_001300333.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8664bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	hyperplastic discs, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGTTTCCA TGCAATTTGT TTTGCAACCG CTACCGGGCT CGGACGATCA GTTTATTGAA 
AGGATTCGTG AAGTGTCGGA TAAGGTGAAC CGATTTGGTT ATGGCAGCCA TCGCATATTC 
GAACAGTTGA AAATTCCAGT GAAGGAAGTC GTCATTGGTC CCGCGCACAT TGGAGTTTTA 
CTGGAGGATG GCAAGGCATT CCGCGTGTCC TTTTCTATAA ATTCTGAGAA ACTTGACTTG 
ACCAAATCAG ACGCAAAATG TTCCACCAGT GGTGGCAGCG GAACGGCCAG TGCTTCGAAA 
GCCCCATCCT CATCCCGACC GATGGCCCGC TCAAGAGCAC GCCTGCTGCG CGCAACAGGT 
CGCTCCAATT CCACCGGACA GGGGTCCGGA TCTCGTAGCA CAGGGGTCAT CATTGGAGGC 
AGTACATCGA GCCGACCCTT AGTAACAGTT CCGGCTACAT ATGTGCCGGA GGAGCTCATA 
TCCCAAGCAG AGGTGGTTCT ACAGGGCAAG AGCAGAAATC TCATAATTAG AGAATTACAG 
CGCACCAACC TCGATGTTAA CTTAGCTGTG AACAATTTGC TTTCGCGCGA TGATGAAGAA 
GCTGAAGATA CCGAAGAAGG TGCCGATAAT TACGTGCCCG AAGACCTTAT CTCCCTGCTG 
GATAATGGGT TCAGTGGAGA TAACAACAGC GTCATCATAG ATCCCTCAGA TGGGCTTTTC 
TCGGAGGAGA TATTTAGCAA CTACTCCAGC ATTCGAAACT TGCTGTTCGA TCGCATTCGT 
AGTGAACGCA GTAATGCCAA CGCAAATGCC GCAGACAGTA ACCAATCGAC GACCCGCTCA 
ACAAGTTCAG GCACTGCCTT AACTGGTAAC AGTGGACTTT CAGCTCAAAT ATCAGTTAAT 
GCTGACCGCG AGGCTTTTAG TCGTTGGCGG GATCGTCAGT ACTACGGACC ACGACGCTGG 
ATAAGCAAAG ACGACTATAC CTGGGAGAAG GATGCTGATT CCAAGAAAAA AGAGCCATCC 
CCCATGTTGT CCCCCATTTG GATATCTGAA GAATTGCAGC CATGGCCAGA GAAAAGTTCC 
GTAAGGTTCA AGACCATAGG AGCTTTGTAT TCGGAGTTCA TTGCCTTGTC AGAGAGCGGA 
GATTTGTACC AGTGGCGCTG GTCAGATGCA GAGCCTTATA AGTCGGAGAC TGAAAACGTT 
TATCATCCCA AAACTGTTTC CCTCAACATA GTTGAAAGAG TGGAGCTTAT TTCGGCCAAC 
TTCATCAGAT GCTCCGTTGT TACAGAAACC AATCGAGTAG CAACTTGGAT GGACGAGCAA 
CTAGGTTACA TCGGCGCCAA GCTGGAGCAC AGTTGCTGCG CGTTTAACGA GTTTATATCG 
GACTCCATAA CAAAGATCTA CGTCTGCTCG CTATACACGG TTGTTAAGAC CGAAAGCAAC 
AACATATATT GGTGGGGAGT CCTGCCGTTT GATCAACGCC GCTTCTTGTG GGACAAATTC 
CGGACCAAGA CCAAGAAACC CTTTAAGGTG GTTGCCACCG ATATTAATGT GGGTGCTCAA 
GTGATAATGA AGAAGTGTCC CATGTATCAA TCTGGATCAA TCGGATTCAC ATGCTCCAAT 
GGCGTCCCAA AGGTGGGGCA GCTTTTGAAT TCAGTCTGGA CCTTCACCGA TGTGTGCCGC 
ATGAAAATCA TAAATATTAA CACGAACTCT GGCGTAGACA AATCCCAAGC AGCCGGCAAT 
AATCTTAATG CCCATGGAAT TACTCCGGAT AAAGATCTGC CAAAGTCCAC GGCAATGCCC 
AGCACTGGAA GTAGTAAGAA TGGACAATCC TTCTCAAACA GCAAGGAGTC AACAGATCGC 
ATCGACATGC CACCTCCGCC TTCACCGGCA TCCAGCACAT GCAGCGACAC CGGCAGCGTC 
ACATCCCACA AGCGTACTAA GCGAGCTACT ACGAAAGAGG ACTCCAATGC CCCGCAGGAG 
GGAAGGAAGG ATGAGGAGCT GTGGGAAGTG AAGGACGTGG TGTTTGTCGA GGACAAGGTG 
GGCCCAGTGG GAAAGGTGCT TAAGGTCGAC GGCGACTTTG TTGCCGTTCG TTTTCCGGCA 
ATAAATGCAG CAGCCGTTGC TGCTGCTGCT GCCGCAACAA GTTCCACATC TAACACAGCC 
TCCACATCCA AGGAAGAGGG CAAGGAGGAC GACTGGCAGC AGTGTCGGCT GCTGAGACGC 
GAAGATGTCC AGATATTTCG CACTGCTATG TCCACCCGTG GCCCAGACTG GTTGCAAAAA 
CAGCCGAAAA AGATTAACGT TGGTGGTGAT GCAGCTGGCG CACAAATACT CACCCTGGCC 
GTGGACTCGC GCGGCATTCA TGTGATCAAG AAGGTTCTCG GCAAAATCCA CTACAGCCTT 
TACAACCTGT ATAACTGCAA GCAGGAGCAA AATTGCCTAT TTCCCACGGA CTGCAACTCT 
TTTATTGGCT CCACGCCTGG CAACATATTA ATGGCTTGCA ACGACGACTG CAGCGGAAAC 
AGCAGTACCA TTGTGCTTCG TGATGGCAAT GGAGCCCTAT ATCCCTTGGC CAAGGATTGC 
CTTGGCTCGA TCAAGGATCC GCAATGGTTT GATCTTCCTC CGGTGAAGTC AATCACTATG 
TCCACAATCT CCCTGCCGGC CATGCTCTCC GGCGTGAATT TGAAGTCAAA GGTATGCATG 
ACTGCTTTGC TCTTTGATAC TCAAAAGCTT ATGCCGCACA TCTTGCGGTG CGATGTCAAG 
AATAGCTTTG CCGCCCTCGG TCGCCTGGAG AGGGAGGATC AAGCGGATAC GGCTCTTGTC 
GTAGAAGAAC GTTGCGATGG AGCCCGAAAC ATATTCCACG CTTGCGTAAT TATGTGCGCC 
CCATCCTCAA ACAAGGATTC CCCACCAGAC TCTCCCAGCG GTGGTGTCGA GAAGAAATCA 
CTAGTGGGAC TCTCCGTGGC GCGTTCCATT CCAACTGTCT CGACAAGCGC TTACGTTAGC 
AGCATCGCAT TTGGAGCAAG TGCGTCTTCG AGCAATGAAA ACTCCAGCTT TGCAACTATG 
AGTTCATCCG CTGCCGGCTC CGCCTCATCG ACGAGCAGGG ATAATCGTAC CAATCTGCGC 
GACATGATGA ATCGTCTAAT AAATAGCGAT CAGGCTGAAC AGTCTGGCAG CCAGCCAATG 
GCTACAAACA ACGAAGACCA TGCCTACATC CCCTGGCCAG CTGAAACACC GGCGGCTAGT 
AATCTGAGCG CCAGCTCTTC GCAGAATGTC TCAGACAGCA TTGAAGATGA TATCTCAAAG 
ATAATTCCGT CCTCCTCCCA GAGTTCCATG CTCTCCAACA TCAAGTTGGG GTCCCCTACC 
TACACCTTCG ACCTGGCTCA GCGGCGGGAG CATGCTTTGA CAATCCTTCA GCAGATGTGC 
GTAAGTCCAG CCCTTCGTCC GTACCTCTGC CATATGCTGA GCACTAAGGA TGCGCAGGGC 
CAAACGCCGT TTATGCTATC CGTTTCGTGT CGCGCCTACG AGGCAGGAAT CATACTACTA 
AACACGATTC TCATGCTGTC CGAGCAGGAT CCGCAGCTAA AGGAGGCCAT GATTTTCCCG 
AATGGTTCAC CTGCGGATCA GTCACCTCTG CATGTCATCT GCTACAATGA TACCTGCTCG 
TTCACATGGA CAGGAGCCGA TCACATCAAC CAGAACATTT TCGAGTGCAA GACATGCGGC 
CTCACTGGAT CCCTGTGCTG CTGCACGGAA TGCGCGAGAG TCTGCCACAA AGGACACGAC 
TGCAAGTTGA AGCGTACGGC GCCGACTGCT TACTGTGATT GTTGGGAGAA ATGCAAGTGT 
AAGGCTCTGA TCGCTGGAAA TCTCACAAAG CGCTTCGCAC TGCTGTGCAA ACTAGTATCC 
TGCACAGATT TGGTCACCAA ATTTAACTCC AAGGGTGAAT CGATTCTTCT TTTCCTTATT 
CAAACGGTTG GACGCCAGAT TGTAGAGCAG CGACAGTACC GCTTTAGTGT AAGGGTTCGC 
AATGTTAGCA CCGCTGCCAC CGGAGCGACC GGAAACAATT CAGTGATATC AAATCGGAAG 
ACCTCCGCAG CCGAAATTGA TAATGATATG CCGGACCATG ATCTGGAGCC ACCGAAGTTC 
GCCAGAAAGG CTTTGGAGAG ACTACTGATC GACTGGAACG CCGTGCGCTC AATGATCATG 
AGCGGTGCGG AGAGAGGCGA TGTACCGAAT CCAGCAGGCA GTGCATCGGA GAACTCTAAT 
TCGGAAGGCT TTAATATGTT TATCCAGACG CAGCATGGCT CTACGCTTCT CGACAAGTTT 
ACCCACAGTT TGATCGTAAA GTGCACTAGC GATCATTTGG ACACTTTGCT ACTCACCCTG 
GTGCGCGAAT TGCAGAACGC AAGCGTGTCC AATCGTAGCA AGGAAGCTGA GGAAGTAGTG 
CGTCGCTTCG TGCGTTCCGT AGCACGCGTT TTTGTCATTT TCAACCTGGA AAAGCAGCCT 
AATCCGGAGA AGCGAAAGTC GCACTCATCC TGCAACAAAT ACGTGCAGAG CTGTGTTAAA 
GTATTCCAAA CGCTGCACAA GATATCCATC GAGGAGTTAT GTGAGGTATC CGAGGCTCTG 
ATCGCTCCAG TCCGACTGGG CGTTGTGCGT CCAACAGCTC CGTTTACGAT GTCATCGTCG 
AATCTGGATG TACGTAACTC TGACGATTTG TTCAGCGTTG ATCCTTTAGC CCCCTCTAAC 
GTGGAGTCTC CTTCTGAGCA AATACTTGTC CACGATGCTG GAAACGATCA GAGCGCCAAT 
TTTAATATTC AACAAAACTA CGATGTAGTT GCTATGGAAA CTATACGCGA CGCCTCCGAA 
TCGGAAGAAG TTATAAACCG GGAGGCTAAT TCCCACAATC AAGACGATGA GCTAATTGAA 
AATCAACGAA ATGAGGACGG CATGCAAGAT GACGAGAGCG ATAATGACTT TACTTTCAAT 
GACGCCGAGA CTGAGTCCGA CTCCGATGAC AACCAAAGCA ATCAAGAGGT GCAGAGGAGC 
GTGCAAGCAG GAGCAACCGT TGGCTCAGAA AATGACATTG GTGTTCTCTT CCTAGAGGAT 
GAATCGGGAG ACTCCTCTGC CCAAGAAGAA GATGGATCGG AGGATGGCGA GTCCGACGAT 
CAGTCCGACG AGTTCAATTT CAATGAGCAG CAACTTGAAC GCCGCTCTAC CAACTCGAAC 
GCTCGCAGCG ATCTGGCTCC TCAAACGATG CAATGGGCCA TACGCAGTCG TGACACTGCG 
CGCTCCTCCG TGAGGGTGCC CACCGGGTCT AATATGGTTT TTATTGACCC CATGGCTCTG 
CGCCGCTCCA CTGTACCCGC CAGCACTACA GTCACTACCC CATCGATTGA ACCACACACC 
ATGGCGACAA CGGCCAGCAA CTTGGCACGG GCTTTCGGAA TCACAATAAG GCAAATATCC 
GAGCTTATAA GCATTTTATC CTATAACGTA CTAAATGACA TCGAAACATC ATTAAAAATT 
CAAAATGATG AAGCAATCGC AGTCCAAGCA TTTGTGGAAA AGCGTTTAAA GGCTACGTGG 
GATTGGATGT TTACGGTAAT GGACGGTACC GAAGCACAGC TAAAGTTCGG AGCTTATTTG 
ACCAACTACA CGGATCCCAA CCATCCGCTG CATCCGCTCA ACCTAAGTGC CCAGGCTTCG 
AGTAGCCAGA CTCCGGCTCC TGCCACATCG TCGTCAGTTA ATGGTGTAAA TATTATGGGA 
TCCAATTCAC GCCGCGACTT CTTCACCTAC TGTTTATCCC TGATGCGCTC ACACACCTCT 
GAGCACCGAG ACGCCTTGCC AGTGTTGGAT ATAACAGCCT TGCGGCACAT TGCCTATGTA 
CTCGATGCCT TTGTCTACTA TATGCGCAAT GATTCAGGCT TCTATGATAA GCAAGATACC 
ATTTCTGGCC GTATAAACAA TTTGTCACCT ATGACTGAGA GCTATGATAC GGACGACGAG 
TTGGCTAATC TAGAGGAGTT TAACGCCGAT GTTCAGATGT CCGCTAGCAG CATGCCTTCA 
GGAAGCCAGG GCACACGCCG CCATGCATTC TTTGCTCGAT CTGAATCGAC ATTAAGCTTG 
GGCTGCTCAG CGCCAGAAGG CTTTGAATTG CCACTGGATA TGGCCATGCC TCTCGCCGAT 
AAGCCGCACT TGCTACAACC AAATTCTAAG CGTCAGGAAC TCTTCGCAAA CCTACCGCTT 
CTCGTGACCA CGAATGCCAA CAACAGCGGT GCCACTAATG ATGGTGATGG TGGCAGCATT 
TTTGATTATA CTCCAACTAG ACTGGGATTC TCGAATTCCT TGAAAAGGAA CGAGCGTGTT 
TACGAAACAG TCCCGATCGA TTCTAGTAAG ACGGGTGATG GAAATGTAAC TAATAAGGCT 
GAGGGCTCAA CAGACTCTAA TATTTACGTA CAATTAAAGA AGAAACAGGG CTCGGATGAT 
TTCAAATCCC ACAAAGAGGC TGACGGCAAT CAAAGCAAGT ACGAAAAAGT CGTATTAATG 
GAAACTGATG ACAGTCTTCC AAGTACAAGT AAATCGACTG AAGCATTAAT GGCCACACGA 
CCAGAGGTTA TCATAGCGCC AAATAAAGCC TCAGTGTCGC CAGCGACTGC CGCTCGCAGC 
GTCATAGTTC TCGCAGGTGG TTCCTGTTTA AAGACAATTG ATTCCGACAT AAACAACTAC 
TCGGCTTCAA ATCTATCGAC CGCAGAACAA GCCAAATGTG ATACACAATA TCAAAAGTCA 
ACATCAGACC ACCTGTTATT ATTTCCTGCT CGTGGTTCTC AGTTTTACCA AAGTAATTTT 
TCGGAATTGC CGTCGTGGAA CTTTTTACTT AGCCGCTGGA AGCTAACTCT GGACTTATTC 
GGTCGCGTTT TTATGGACGA CGTCGGAATG GAACACGGCT CTGTGCTGCC GGAACTGCGA 
GGCTTTCCCG TGAAGGAGAT GCGTTTCCGT CGCCACATGG AAAAATTGCG CAACGGACAA 
CAACGTGATT TGGTGCTTTG CAAGTTGGAG CGCAATCGTG AAAGCTTAAT TGTTCAAACG 
TTCAAGGAAC TGAATACCCA GTTTGGCAAC CAAAGTCGGC GCATCCAGCC GCCCATCACA 
TTCAACAGAG TAAAGGTGAC ATTCAAAGAC GAGCCGGGCG AGGGATCGGG TGTAGCGCGC 
AGTTTTTACA CATCGATCGC AGAAGCATTG CTGGCCAGTG CTAAGATCCC CAACTTGGAG 
TCCGTACAGG TGGGCACCAA TCATAGCAAG TACGTAGTGC CGTTCTCGAG CATCCTGCGC 
AGCAGAACAG TATCGGGGTC TAGTCGCGAT CAGTCCACTC TGCAGAGGCG TGGTTCCAAC 
AGCAAAATAT TGTGGCGTTC GGCCCGTGAG AGGAAGGCAT TGAATTTGGA TGCCAGACCG 
TATACTCCAC CAAATAGCTC AGATAATGCT ACGCCGGAAA GCCTGAATGA TCACTTGTCC 
GTTCACCTAC AACAAATTGG GGAACGTCTT TACCCGAAGA TCCATTCGAT AAACCAAACG 
CATGCACCGA AGATAACAGG AATGCTGCTG GAGATACCCA CTCCGCAGCT CCTGTCCGTA 
ATTTCCTCAG ATGAGACGCT CCGTCAGAAA GTGAATGAAG CGATAGAAAT AATCACGTTT 
AAACAAAAAT CTGAGACCAG TGCACAAAGT AGTCAACCAA AAAAATCACC ATCTGTCGTG 
GTAGTGGATC CCGTTGATGA TGATAATGAA CCATTATTTT ACTCACCGGG AAAGCGGGGC 
TTTTACACAC CACGCCAAGG CTTTGCATCA TTCGAGCGCA TTAATGCATT TAGAAATATT 
GGAAGACTTA TTGGACTTTG TCTACTGCAA AACGAATTGC TTCCACTGTT CCTACAAAGA 
CATGTACTAA AATACATTCT TGGGCGTAAA ATTAAGTTCC ACGACTTGGC ATTTTTCGAT 
CCAGCATTGT ACGAATCTTT TAGACAAATT ATTCAAAATG CCCAAACAAA GGAAGGCGAG 
GAAACTATAA ATCGTATGGA GCTATGCTTT GTTATCGATT TGATGAAGGA GGAGGGCTGT 
GGTAACAGAG AGCTTATCCC AGGAGGGCGC GATGTCGCTG TCACATCGAG TAATATATTC 
GAGTACGTCA GACGCTATAC AGAATATAGA CTAATCAAAT CCCAAGAAAA GGCACTGGAG 
GCACTGAAGG ACGGAGTTTT TGACGTTTTG CCCGATAACA GCATGATTAA CTTAACCGCT 
GAGGACTTGC GTCTGCTGCT AAACGGTGTT GGTGATATAA ATGTTTCGAC ACTAATTTCA 
TACACTACCT TTAACGACGA ATCTAGCGAG GGTCCTGATA AACTTTTAAA ATTTAAGAAA 
TGGTTTTGGA GTATTGTGGA GAAAATGAAC ATTATGGAAC GCCAGGATTT GGTTTACTTC 
TGGACCGGCT CTCCTGCGCT TCCAGCTTCG GAGGAGGGAT TCCAACCATT GCCATCTGTC 
ACCATAAGAC CTGCGGATGA CTCCCATCTA CCGACTGCGA ATACTTGTAT CTCTCGGCTG 
TATATCCCTC TTTACTCCAG CAAATCTATT TTACGCAGTA AAATGCTGAT GGCCATTAAA 
TCCAAAAATT TTGGATTTGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287262.1
CDS	91..8754
Translation	MVSMQFVLQPLPGSDDQFIERIREVSDKVNRFGYGSHRIFEQLKIPVKEVVIGPAHIGVLLEDGKAFRVSFSINSEKLDLTKSDAKCSTSGGSGTASASKAPSSSRPMARSRARLLRATGRSNSTGQGSGSRSTGVIIGGSTSSRPLVTVPATYVPEELISQAEVVLQGKSRNLIIRELQRTNLDVNLAVNNLLSRDDEEAEDTEEGADNYVPEDLISLLDNGFSGDNNSVIIDPSDGLFSEEIFSNYSSIRNLLFDRIRSERSNANANAADSNQSTTRSTSSGTALTGNSGLSAQISVNADREAFSRWRDRQYYGPRRWISKDDYTWEKDADSKKKEPSPMLSPIWISEELQPWPEKSSVRFKTIGALYSEFIALSESGDLYQWRWSDAEPYKSETENVYHPKTVSLNIVERVELISANFIRCSVVTETNRVATWMDEQLGYIGAKLEHSCCAFNEFISDSITKIYVCSLYTVVKTESNNIYWWGVLPFDQRRFLWDKFRTKTKKPFKVVATDINVGAQVIMKKCPMYQSGSIGFTCSNGVPKVGQLLNSVWTFTDVCRMKIININTNSGVDKSQAAGNNLNAHGITPDKDLPKSTAMPSTGSSKNGQSFSNSKESTDRIDMPPPPSPASSTCSDTGSVTSHKRTKRATTKEDSNAPQEGRKDEELWEVKDVVFVEDKVGPVGKVLKVDGDFVAVRFPAINAAAVAAAAAATSSTSNTASTSKEEGKEDDWQQCRLLRREDVQIFRTAMSTRGPDWLQKQPKKINVGGDAAGAQILTLAVDSRGIHVIKKVLGKIHYSLYNLYNCKQEQNCLFPTDCNSFIGSTPGNILMACNDDCSGNSSTIVLRDGNGALYPLAKDCLGSIKDPQWFDLPPVKSITMSTISLPAMLSGVNLKSKVCMTALLFDTQKLMPHILRCDVKNSFAALGRLEREDQADTALVVEERCDGARNIFHACVIMCAPSSNKDSPPDSPSGGVEKKSLVGLSVARSIPTVSTSAYVSSIAFGASASSSNENSSFATMSSSAAGSASSTSRDNRTNLRDMMNRLINSDQAEQSGSQPMATNNEDHAYIPWPAETPAASNLSASSSQNVSDSIEDDISKIIPSSSQSSMLSNIKLGSPTYTFDLAQRREHALTILQQMCVSPALRPYLCHMLSTKDAQGQTPFMLSVSCRAYEAGIILLNTILMLSEQDPQLKEAMIFPNGSPADQSPLHVICYNDTCSFTWTGADHINQNIFECKTCGLTGSLCCCTECARVCHKGHDCKLKRTAPTAYCDCWEKCKCKALIAGNLTKRFALLCKLVSCTDLVTKFNSKGESILLFLIQTVGRQIVEQRQYRFSVRVRNVSTAATGATGNNSVISNRKTSAAEIDNDMPDHDLEPPKFARKALERLLIDWNAVRSMIMSGAERGDVPNPAGSASENSNSEGFNMFIQTQHGSTLLDKFTHSLIVKCTSDHLDTLLLTLVRELQNASVSNRSKEAEEVVRRFVRSVARVFVIFNLEKQPNPEKRKSHSSCNKYVQSCVKVFQTLHKISIEELCEVSEALIAPVRLGVVRPTAPFTMSSSNLDVRNSDDLFSVDPLAPSNVESPSEQILVHDAGNDQSANFNIQQNYDVVAMETIRDASESEEVINREANSHNQDDELIENQRNEDGMQDDESDNDFTFNDAETESDSDDNQSNQEVQRSVQAGATVGSENDIGVLFLEDESGDSSAQEEDGSEDGESDDQSDEFNFNEQQLERRSTNSNARSDLAPQTMQWAIRSRDTARSSVRVPTGSNMVFIDPMALRRSTVPASTTVTTPSIEPHTMATTASNLARAFGITIRQISELISILSYNVLNDIETSLKIQNDEAIAVQAFVEKRLKATWDWMFTVMDGTEAQLKFGAYLTNYTDPNHPLHPLNLSAQASSSQTPAPATSSSVNGVNIMGSNSRRDFFTYCLSLMRSHTSEHRDALPVLDITALRHIAYVLDAFVYYMRNDSGFYDKQDTISGRINNLSPMTESYDTDDELANLEEFNADVQMSASSMPSGSQGTRRHAFFARSESTLSLGCSAPEGFELPLDMAMPLADKPHLLQPNSKRQELFANLPLLVTTNANNSGATNDGDGGSIFDYTPTRLGFSNSLKRNERVYETVPIDSSKTGDGNVTNKAEGSTDSNIYVQLKKKQGSDDFKSHKEADGNQSKYEKVVLMETDDSLPSTSKSTEALMATRPEVIIAPNKASVSPATAARSVIVLAGGSCLKTIDSDINNYSASNLSTAEQAKCDTQYQKSTSDHLLLFPARGSQFYQSNFSELPSWNFLLSRWKLTLDLFGRVFMDDVGMEHGSVLPELRGFPVKEMRFRRHMEKLRNGQQRDLVLCKLERNRESLIVQTFKELNTQFGNQSRRIQPPITFNRVKVTFKDEPGEGSGVARSFYTSIAEALLASAKIPNLESVQVGTNHSKYVVPFSSILRSRTVSGSSRDQSTLQRRGSNSKILWRSARERKALNLDARPYTPPNSSDNATPESLNDHLSVHLQQIGERLYPKIHSINQTHAPKITGMLLEIPTPQLLSVISSDETLRQKVNEAIEIITFKQKSETSAQSSQPKKSPSVVVVDPVDDDNEPLFYSPGKRGFYTPRQGFASFERINAFRNIGRLIGLCLLQNELLPLFLQRHVLKYILGRKIKFHDLAFFDPALYESFRQIIQNAQTKEGEETINRMELCFVIDLMKEEGCGNRELIPGGRDVAVTSSNIFEYVRRYTEYRLIKSQEKALEALKDGVFDVLPDNSMINLTAEDLRLLLNGVGDINVSTLISYTTFNDESSEGPDKLLKFKKWFWSIVEKMNIMERQDLVYFWTGSPALPASEEGFQPLPSVTIRPADDSHLPTANTCISRLYIPLYSSKSILRSKMLMAIKSKNFGFV

Target ORF information:

RefSeq Version	NM_001300333.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster hyperplastic discs (hyd), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300333.1

ORF Insert Sequence:

ATGGTTTCCA TGCAATTTGT TTTGCAACCG CTACCGGGCT CGGACGATCA GTTTATTGAA 
AGGATTCGTG AAGTGTCGGA TAAGGTGAAC CGATTTGGTT ATGGCAGCCA TCGCATATTC 
GAACAGTTGA AAATTCCAGT GAAGGAAGTC GTCATTGGTC CCGCGCACAT TGGAGTTTTA 
CTGGAGGATG GCAAGGCATT CCGCGTGTCC TTTTCTATAA ATTCTGAGAA ACTTGACTTG 
ACCAAATCAG ACGCAAAATG TTCCACCAGT GGTGGCAGCG GAACGGCCAG TGCTTCGAAA 
GCCCCATCCT CATCCCGACC GATGGCCCGC TCAAGAGCAC GCCTGCTGCG CGCAACAGGT 
CGCTCCAATT CCACCGGACA GGGGTCCGGA TCTCGTAGCA CAGGGGTCAT CATTGGAGGC 
AGTACATCGA GCCGACCCTT AGTAACAGTT CCGGCTACAT ATGTGCCGGA GGAGCTCATA 
TCCCAAGCAG AGGTGGTTCT ACAGGGCAAG AGCAGAAATC TCATAATTAG AGAATTACAG 
CGCACCAACC TCGATGTTAA CTTAGCTGTG AACAATTTGC TTTCGCGCGA TGATGAAGAA 
GCTGAAGATA CCGAAGAAGG TGCCGATAAT TACGTGCCCG AAGACCTTAT CTCCCTGCTG 
GATAATGGGT TCAGTGGAGA TAACAACAGC GTCATCATAG ATCCCTCAGA TGGGCTTTTC 
TCGGAGGAGA TATTTAGCAA CTACTCCAGC ATTCGAAACT TGCTGTTCGA TCGCATTCGT 
AGTGAACGCA GTAATGCCAA CGCAAATGCC GCAGACAGTA ACCAATCGAC GACCCGCTCA 
ACAAGTTCAG GCACTGCCTT AACTGGTAAC AGTGGACTTT CAGCTCAAAT ATCAGTTAAT 
GCTGACCGCG AGGCTTTTAG TCGTTGGCGG GATCGTCAGT ACTACGGACC ACGACGCTGG 
ATAAGCAAAG ACGACTATAC CTGGGAGAAG GATGCTGATT CCAAGAAAAA AGAGCCATCC 
CCCATGTTGT CCCCCATTTG GATATCTGAA GAATTGCAGC CATGGCCAGA GAAAAGTTCC 
GTAAGGTTCA AGACCATAGG AGCTTTGTAT TCGGAGTTCA TTGCCTTGTC AGAGAGCGGA 
GATTTGTACC AGTGGCGCTG GTCAGATGCA GAGCCTTATA AGTCGGAGAC TGAAAACGTT 
TATCATCCCA AAACTGTTTC CCTCAACATA GTTGAAAGAG TGGAGCTTAT TTCGGCCAAC 
TTCATCAGAT GCTCCGTTGT TACAGAAACC AATCGAGTAG CAACTTGGAT GGACGAGCAA 
CTAGGTTACA TCGGCGCCAA GCTGGAGCAC AGTTGCTGCG CGTTTAACGA GTTTATATCG 
GACTCCATAA CAAAGATCTA CGTCTGCTCG CTATACACGG TTGTTAAGAC CGAAAGCAAC 
AACATATATT GGTGGGGAGT CCTGCCGTTT GATCAACGCC GCTTCTTGTG GGACAAATTC 
CGGACCAAGA CCAAGAAACC CTTTAAGGTG GTTGCCACCG ATATTAATGT GGGTGCTCAA 
GTGATAATGA AGAAGTGTCC CATGTATCAA TCTGGATCAA TCGGATTCAC ATGCTCCAAT 
GGCGTCCCAA AGGTGGGGCA GCTTTTGAAT TCAGTCTGGA CCTTCACCGA TGTGTGCCGC 
ATGAAAATCA TAAATATTAA CACGAACTCT GGCGTAGACA AATCCCAAGC AGCCGGCAAT 
AATCTTAATG CCCATGGAAT TACTCCGGAT AAAGATCTGC CAAAGTCCAC GGCAATGCCC 
AGCACTGGAA GTAGTAAGAA TGGACAATCC TTCTCAAACA GCAAGGAGTC AACAGATCGC 
ATCGACATGC CACCTCCGCC TTCACCGGCA TCCAGCACAT GCAGCGACAC CGGCAGCGTC 
ACATCCCACA AGCGTACTAA GCGAGCTACT ACGAAAGAGG ACTCCAATGC CCCGCAGGAG 
GGAAGGAAGG ATGAGGAGCT GTGGGAAGTG AAGGACGTGG TGTTTGTCGA GGACAAGGTG 
GGCCCAGTGG GAAAGGTGCT TAAGGTCGAC GGCGACTTTG TTGCCGTTCG TTTTCCGGCA 
ATAAATGCAG CAGCCGTTGC TGCTGCTGCT GCCGCAACAA GTTCCACATC TAACACAGCC 
TCCACATCCA AGGAAGAGGG CAAGGAGGAC GACTGGCAGC AGTGTCGGCT GCTGAGACGC 
GAAGATGTCC AGATATTTCG CACTGCTATG TCCACCCGTG GCCCAGACTG GTTGCAAAAA 
CAGCCGAAAA AGATTAACGT TGGTGGTGAT GCAGCTGGCG CACAAATACT CACCCTGGCC 
GTGGACTCGC GCGGCATTCA TGTGATCAAG AAGGTTCTCG GCAAAATCCA CTACAGCCTT 
TACAACCTGT ATAACTGCAA GCAGGAGCAA AATTGCCTAT TTCCCACGGA CTGCAACTCT 
TTTATTGGCT CCACGCCTGG CAACATATTA ATGGCTTGCA ACGACGACTG CAGCGGAAAC 
AGCAGTACCA TTGTGCTTCG TGATGGCAAT GGAGCCCTAT ATCCCTTGGC CAAGGATTGC 
CTTGGCTCGA TCAAGGATCC GCAATGGTTT GATCTTCCTC CGGTGAAGTC AATCACTATG 
TCCACAATCT CCCTGCCGGC CATGCTCTCC GGCGTGAATT TGAAGTCAAA GGTATGCATG 
ACTGCTTTGC TCTTTGATAC TCAAAAGCTT ATGCCGCACA TCTTGCGGTG CGATGTCAAG 
AATAGCTTTG CCGCCCTCGG TCGCCTGGAG AGGGAGGATC AAGCGGATAC GGCTCTTGTC 
GTAGAAGAAC GTTGCGATGG AGCCCGAAAC ATATTCCACG CTTGCGTAAT TATGTGCGCC 
CCATCCTCAA ACAAGGATTC CCCACCAGAC TCTCCCAGCG GTGGTGTCGA GAAGAAATCA 
CTAGTGGGAC TCTCCGTGGC GCGTTCCATT CCAACTGTCT CGACAAGCGC TTACGTTAGC 
AGCATCGCAT TTGGAGCAAG TGCGTCTTCG AGCAATGAAA ACTCCAGCTT TGCAACTATG 
AGTTCATCCG CTGCCGGCTC CGCCTCATCG ACGAGCAGGG ATAATCGTAC CAATCTGCGC 
GACATGATGA ATCGTCTAAT AAATAGCGAT CAGGCTGAAC AGTCTGGCAG CCAGCCAATG 
GCTACAAACA ACGAAGACCA TGCCTACATC CCCTGGCCAG CTGAAACACC GGCGGCTAGT 
AATCTGAGCG CCAGCTCTTC GCAGAATGTC TCAGACAGCA TTGAAGATGA TATCTCAAAG 
ATAATTCCGT CCTCCTCCCA GAGTTCCATG CTCTCCAACA TCAAGTTGGG GTCCCCTACC 
TACACCTTCG ACCTGGCTCA GCGGCGGGAG CATGCTTTGA CAATCCTTCA GCAGATGTGC 
GTAAGTCCAG CCCTTCGTCC GTACCTCTGC CATATGCTGA GCACTAAGGA TGCGCAGGGC 
CAAACGCCGT TTATGCTATC CGTTTCGTGT CGCGCCTACG AGGCAGGAAT CATACTACTA 
AACACGATTC TCATGCTGTC CGAGCAGGAT CCGCAGCTAA AGGAGGCCAT GATTTTCCCG 
AATGGTTCAC CTGCGGATCA GTCACCTCTG CATGTCATCT GCTACAATGA TACCTGCTCG 
TTCACATGGA CAGGAGCCGA TCACATCAAC CAGAACATTT TCGAGTGCAA GACATGCGGC 
CTCACTGGAT CCCTGTGCTG CTGCACGGAA TGCGCGAGAG TCTGCCACAA AGGACACGAC 
TGCAAGTTGA AGCGTACGGC GCCGACTGCT TACTGTGATT GTTGGGAGAA ATGCAAGTGT 
AAGGCTCTGA TCGCTGGAAA TCTCACAAAG CGCTTCGCAC TGCTGTGCAA ACTAGTATCC 
TGCACAGATT TGGTCACCAA ATTTAACTCC AAGGGTGAAT CGATTCTTCT TTTCCTTATT 
CAAACGGTTG GACGCCAGAT TGTAGAGCAG CGACAGTACC GCTTTAGTGT AAGGGTTCGC 
AATGTTAGCA CCGCTGCCAC CGGAGCGACC GGAAACAATT CAGTGATATC AAATCGGAAG 
ACCTCCGCAG CCGAAATTGA TAATGATATG CCGGACCATG ATCTGGAGCC ACCGAAGTTC 
GCCAGAAAGG CTTTGGAGAG ACTACTGATC GACTGGAACG CCGTGCGCTC AATGATCATG 
AGCGGTGCGG AGAGAGGCGA TGTACCGAAT CCAGCAGGCA GTGCATCGGA GAACTCTAAT 
TCGGAAGGCT TTAATATGTT TATCCAGACG CAGCATGGCT CTACGCTTCT CGACAAGTTT 
ACCCACAGTT TGATCGTAAA GTGCACTAGC GATCATTTGG ACACTTTGCT ACTCACCCTG 
GTGCGCGAAT TGCAGAACGC AAGCGTGTCC AATCGTAGCA AGGAAGCTGA GGAAGTAGTG 
CGTCGCTTCG TGCGTTCCGT AGCACGCGTT TTTGTCATTT TCAACCTGGA AAAGCAGCCT 
AATCCGGAGA AGCGAAAGTC GCACTCATCC TGCAACAAAT ACGTGCAGAG CTGTGTTAAA 
GTATTCCAAA CGCTGCACAA GATATCCATC GAGGAGTTAT GTGAGGTATC CGAGGCTCTG 
ATCGCTCCAG TCCGACTGGG CGTTGTGCGT CCAACAGCTC CGTTTACGAT GTCATCGTCG 
AATCTGGATG TACGTAACTC TGACGATTTG TTCAGCGTTG ATCCTTTAGC CCCCTCTAAC 
GTGGAGTCTC CTTCTGAGCA AATACTTGTC CACGATGCTG GAAACGATCA GAGCGCCAAT 
TTTAATATTC AACAAAACTA CGATGTAGTT GCTATGGAAA CTATACGCGA CGCCTCCGAA 
TCGGAAGAAG TTATAAACCG GGAGGCTAAT TCCCACAATC AAGACGATGA GCTAATTGAA 
AATCAACGAA ATGAGGACGG CATGCAAGAT GACGAGAGCG ATAATGACTT TACTTTCAAT 
GACGCCGAGA CTGAGTCCGA CTCCGATGAC AACCAAAGCA ATCAAGAGGT GCAGAGGAGC 
GTGCAAGCAG GAGCAACCGT TGGCTCAGAA AATGACATTG GTGTTCTCTT CCTAGAGGAT 
GAATCGGGAG ACTCCTCTGC CCAAGAAGAA GATGGATCGG AGGATGGCGA GTCCGACGAT 
CAGTCCGACG AGTTCAATTT CAATGAGCAG CAACTTGAAC GCCGCTCTAC CAACTCGAAC 
GCTCGCAGCG ATCTGGCTCC TCAAACGATG CAATGGGCCA TACGCAGTCG TGACACTGCG 
CGCTCCTCCG TGAGGGTGCC CACCGGGTCT AATATGGTTT TTATTGACCC CATGGCTCTG 
CGCCGCTCCA CTGTACCCGC CAGCACTACA GTCACTACCC CATCGATTGA ACCACACACC 
ATGGCGACAA CGGCCAGCAA CTTGGCACGG GCTTTCGGAA TCACAATAAG GCAAATATCC 
GAGCTTATAA GCATTTTATC CTATAACGTA CTAAATGACA TCGAAACATC ATTAAAAATT 
CAAAATGATG AAGCAATCGC AGTCCAAGCA TTTGTGGAAA AGCGTTTAAA GGCTACGTGG 
GATTGGATGT TTACGGTAAT GGACGGTACC GAAGCACAGC TAAAGTTCGG AGCTTATTTG 
ACCAACTACA CGGATCCCAA CCATCCGCTG CATCCGCTCA ACCTAAGTGC CCAGGCTTCG 
AGTAGCCAGA CTCCGGCTCC TGCCACATCG TCGTCAGTTA ATGGTGTAAA TATTATGGGA 
TCCAATTCAC GCCGCGACTT CTTCACCTAC TGTTTATCCC TGATGCGCTC ACACACCTCT 
GAGCACCGAG ACGCCTTGCC AGTGTTGGAT ATAACAGCCT TGCGGCACAT TGCCTATGTA 
CTCGATGCCT TTGTCTACTA TATGCGCAAT GATTCAGGCT TCTATGATAA GCAAGATACC 
ATTTCTGGCC GTATAAACAA TTTGTCACCT ATGACTGAGA GCTATGATAC GGACGACGAG 
TTGGCTAATC TAGAGGAGTT TAACGCCGAT GTTCAGATGT CCGCTAGCAG CATGCCTTCA 
GGAAGCCAGG GCACACGCCG CCATGCATTC TTTGCTCGAT CTGAATCGAC ATTAAGCTTG 
GGCTGCTCAG CGCCAGAAGG CTTTGAATTG CCACTGGATA TGGCCATGCC TCTCGCCGAT 
AAGCCGCACT TGCTACAACC AAATTCTAAG CGTCAGGAAC TCTTCGCAAA CCTACCGCTT 
CTCGTGACCA CGAATGCCAA CAACAGCGGT GCCACTAATG ATGGTGATGG TGGCAGCATT 
TTTGATTATA CTCCAACTAG ACTGGGATTC TCGAATTCCT TGAAAAGGAA CGAGCGTGTT 
TACGAAACAG TCCCGATCGA TTCTAGTAAG ACGGGTGATG GAAATGTAAC TAATAAGGCT 
GAGGGCTCAA CAGACTCTAA TATTTACGTA CAATTAAAGA AGAAACAGGG CTCGGATGAT 
TTCAAATCCC ACAAAGAGGC TGACGGCAAT CAAAGCAAGT ACGAAAAAGT CGTATTAATG 
GAAACTGATG ACAGTCTTCC AAGTACAAGT AAATCGACTG AAGCATTAAT GGCCACACGA 
CCAGAGGTTA TCATAGCGCC AAATAAAGCC TCAGTGTCGC CAGCGACTGC CGCTCGCAGC 
GTCATAGTTC TCGCAGGTGG TTCCTGTTTA AAGACAATTG ATTCCGACAT AAACAACTAC 
TCGGCTTCAA ATCTATCGAC CGCAGAACAA GCCAAATGTG ATACACAATA TCAAAAGTCA 
ACATCAGACC ACCTGTTATT ATTTCCTGCT CGTGGTTCTC AGTTTTACCA AAGTAATTTT 
TCGGAATTGC CGTCGTGGAA CTTTTTACTT AGCCGCTGGA AGCTAACTCT GGACTTATTC 
GGTCGCGTTT TTATGGACGA CGTCGGAATG GAACACGGCT CTGTGCTGCC GGAACTGCGA 
GGCTTTCCCG TGAAGGAGAT GCGTTTCCGT CGCCACATGG AAAAATTGCG CAACGGACAA 
CAACGTGATT TGGTGCTTTG CAAGTTGGAG CGCAATCGTG AAAGCTTAAT TGTTCAAACG 
TTCAAGGAAC TGAATACCCA GTTTGGCAAC CAAAGTCGGC GCATCCAGCC GCCCATCACA 
TTCAACAGAG TAAAGGTGAC ATTCAAAGAC GAGCCGGGCG AGGGATCGGG TGTAGCGCGC 
AGTTTTTACA CATCGATCGC AGAAGCATTG CTGGCCAGTG CTAAGATCCC CAACTTGGAG 
TCCGTACAGG TGGGCACCAA TCATAGCAAG TACGTAGTGC CGTTCTCGAG CATCCTGCGC 
AGCAGAACAG TATCGGGGTC TAGTCGCGAT CAGTCCACTC TGCAGAGGCG TGGTTCCAAC 
AGCAAAATAT TGTGGCGTTC GGCCCGTGAG AGGAAGGCAT TGAATTTGGA TGCCAGACCG 
TATACTCCAC CAAATAGCTC AGATAATGCT ACGCCGGAAA GCCTGAATGA TCACTTGTCC 
GTTCACCTAC AACAAATTGG GGAACGTCTT TACCCGAAGA TCCATTCGAT AAACCAAACG 
CATGCACCGA AGATAACAGG AATGCTGCTG GAGATACCCA CTCCGCAGCT CCTGTCCGTA 
ATTTCCTCAG ATGAGACGCT CCGTCAGAAA GTGAATGAAG CGATAGAAAT AATCACGTTT 
AAACAAAAAT CTGAGACCAG TGCACAAAGT AGTCAACCAA AAAAATCACC ATCTGTCGTG 
GTAGTGGATC CCGTTGATGA TGATAATGAA CCATTATTTT ACTCACCGGG AAAGCGGGGC 
TTTTACACAC CACGCCAAGG CTTTGCATCA TTCGAGCGCA TTAATGCATT TAGAAATATT 
GGAAGACTTA TTGGACTTTG TCTACTGCAA AACGAATTGC TTCCACTGTT CCTACAAAGA 
CATGTACTAA AATACATTCT TGGGCGTAAA ATTAAGTTCC ACGACTTGGC ATTTTTCGAT 
CCAGCATTGT ACGAATCTTT TAGACAAATT ATTCAAAATG CCCAAACAAA GGAAGGCGAG 
GAAACTATAA ATCGTATGGA GCTATGCTTT GTTATCGATT TGATGAAGGA GGAGGGCTGT 
GGTAACAGAG AGCTTATCCC AGGAGGGCGC GATGTCGCTG TCACATCGAG TAATATATTC 
GAGTACGTCA GACGCTATAC AGAATATAGA CTAATCAAAT CCCAAGAAAA GGCACTGGAG 
GCACTGAAGG ACGGAGTTTT TGACGTTTTG CCCGATAACA GCATGATTAA CTTAACCGCT 
GAGGACTTGC GTCTGCTGCT AAACGGTGTT GGTGATATAA ATGTTTCGAC ACTAATTTCA 
TACACTACCT TTAACGACGA ATCTAGCGAG GGTCCTGATA AACTTTTAAA ATTTAAGAAA 
TGGTTTTGGA GTATTGTGGA GAAAATGAAC ATTATGGAAC GCCAGGATTT GGTTTACTTC 
TGGACCGGCT CTCCTGCGCT TCCAGCTTCG GAGGAGGGAT TCCAACCATT GCCATCTGTC 
ACCATAAGAC CTGCGGATGA CTCCCATCTA CCGACTGCGA ATACTTGTAT CTCTCGGCTG 
TATATCCCTC TTTACTCCAG CAAATCTATT TTACGCAGTA AAATGCTGAT GGCCATTAAA 
TCCAAAAATT TTGGATTTGT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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hyd cDNA ORF clone, Drosophila melanogaster(fruit fly)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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