man2a2 cDNA ORF clone, Danio rerio(zebrafish)

The following man2a2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the man2a2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa49900 XM_009297800.2
Latest version!
Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa49900 XM_009297801.3
Latest version!
Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa49901 XM_009297802.2
Latest version!
Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa49902 XM_009297803.2
Latest version!
Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa49900 XM_009297800.1 Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa49900 XM_009297801.2 Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa49901 XM_009297802.1 Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa49902 XM_009297803.1 Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa49900
    Clone ID Related Accession (Same CDS sequence) XM_009297800.1 , XM_009297801.2 , XM_009297800.2 , XM_009297801.3
    Accession Version XM_009297800.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product alpha-mannosidase 2x isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC
    CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT
    ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG
    CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT
    CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT
    GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG
    TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG
    ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG
    ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC
    AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT
    GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT
    TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG
    ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG
    CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG
    CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG
    AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC
    GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC
    AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT
    GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG
    GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG
    AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG
    TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG
    GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG
    GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT
    TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA
    CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG
    CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC
    GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT
    AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG
    CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT
    ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT
    CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA
    CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG
    GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG
    TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA
    CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA
    CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG
    CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC
    ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA
    AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG
    TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT
    CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT
    AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG
    GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC
    AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC
    CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG
    GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA
    CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT
    GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA
    GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC
    ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC
    ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC
    AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG
    CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC
    ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG
    CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT
    CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296075.1
    CDS788..4312
    Translation

    Target ORF information:

    RefSeq Version XM_009297800.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297800.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC
    CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT
    ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG
    CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT
    CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT
    GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG
    TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG
    ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG
    ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC
    AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT
    GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT
    TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG
    ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG
    CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG
    CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG
    AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC
    GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC
    AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT
    GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG
    GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG
    AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG
    TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG
    GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG
    GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT
    TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA
    CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG
    CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC
    GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT
    AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG
    CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT
    ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT
    CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA
    CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG
    GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG
    TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA
    CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA
    CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG
    CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC
    ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA
    AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG
    TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT
    CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT
    AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG
    GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC
    AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC
    CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG
    GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA
    CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT
    GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA
    GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC
    ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC
    ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC
    AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG
    CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC
    ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG
    CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT
    CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49900
    Clone ID Related Accession (Same CDS sequence) XM_009297800.1 , XM_009297801.2 , XM_009297800.2 , XM_009297801.3
    Accession Version XM_009297801.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product alpha-mannosidase 2x isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297801.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC
    CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT
    ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG
    CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT
    CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT
    GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG
    TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG
    ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG
    ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC
    AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT
    GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT
    TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG
    ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG
    CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG
    CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG
    AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC
    GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC
    AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT
    GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG
    GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG
    AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG
    TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG
    GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG
    GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT
    TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA
    CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG
    CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC
    GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT
    AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG
    CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT
    ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT
    CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA
    CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG
    GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG
    TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA
    CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA
    CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG
    CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC
    ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA
    AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG
    TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT
    CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT
    AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG
    GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC
    AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC
    CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG
    GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA
    CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT
    GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA
    GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC
    ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC
    ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC
    AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG
    CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC
    ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG
    CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT
    CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296076.1
    CDS772..4296
    Translation

    Target ORF information:

    RefSeq Version XM_009297801.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297801.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC
    CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT
    ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG
    CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT
    CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT
    GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG
    TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG
    ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG
    ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC
    AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT
    GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT
    TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG
    ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG
    CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG
    CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG
    AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC
    GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC
    AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT
    GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG
    GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG
    AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG
    TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG
    GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG
    GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT
    TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA
    CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG
    CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC
    GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT
    AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG
    CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT
    ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT
    CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA
    CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG
    GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG
    TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA
    CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA
    CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG
    CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC
    ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA
    AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG
    TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT
    CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT
    AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG
    GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC
    AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC
    CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG
    GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA
    CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT
    GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA
    GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC
    ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC
    ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC
    AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG
    CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC
    ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG
    CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT
    CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49900
    Clone ID Related Accession (Same CDS sequence) XM_009297800.1 , XM_009297801.2 , XM_009297800.2 , XM_009297801.3
    Accession Version XM_009297800.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alpha-mannosidase 2x isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297800.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC
    CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT
    ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG
    CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT
    CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT
    GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG
    TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG
    ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG
    ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC
    AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT
    GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT
    TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG
    ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG
    CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG
    CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG
    AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC
    GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC
    AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT
    GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG
    GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG
    AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG
    TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG
    GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG
    GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT
    TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA
    CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG
    CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC
    GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT
    AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG
    CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT
    ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT
    CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA
    CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG
    GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG
    TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA
    CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA
    CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG
    CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC
    ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA
    AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG
    TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT
    CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT
    AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG
    GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC
    AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC
    CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG
    GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA
    CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT
    GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA
    GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC
    ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC
    ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC
    AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG
    CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC
    ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG
    CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT
    CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296075.1
    CDS788..4312
    Translation

    Target ORF information:

    RefSeq Version XM_009297800.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297800.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC
    CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT
    ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG
    CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT
    CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT
    GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG
    TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG
    ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG
    ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC
    AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT
    GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT
    TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG
    ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG
    CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG
    CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG
    AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC
    GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC
    AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT
    GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG
    GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG
    AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG
    TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG
    GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG
    GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT
    TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA
    CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG
    CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC
    GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT
    AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG
    CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT
    ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT
    CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA
    CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG
    GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG
    TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA
    CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA
    CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG
    CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC
    ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA
    AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG
    TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT
    CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT
    AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG
    GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC
    AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC
    CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG
    GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA
    CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT
    GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA
    GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC
    ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC
    ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC
    AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG
    CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC
    ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG
    CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT
    CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49900
    Clone ID Related Accession (Same CDS sequence) XM_009297800.1 , XM_009297801.2 , XM_009297800.2 , XM_009297801.3
    Accession Version XM_009297801.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3525bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alpha-mannosidase 2x isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297801.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC
    CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT
    ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG
    CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT
    CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT
    GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG
    TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG
    ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG
    ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC
    AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT
    GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT
    TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG
    ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG
    CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG
    CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG
    AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC
    GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC
    AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT
    GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG
    GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG
    AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG
    TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG
    GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG
    GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT
    TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA
    CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG
    CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC
    GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT
    AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG
    CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT
    ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT
    CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA
    CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG
    GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG
    TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA
    CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA
    CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG
    CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC
    ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA
    AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG
    TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT
    CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT
    AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG
    GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC
    AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC
    CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG
    GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA
    CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT
    GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA
    GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC
    ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC
    ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC
    AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG
    CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC
    ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG
    CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT
    CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296076.1
    CDS780..4304
    Translation

    Target ORF information:

    RefSeq Version XM_009297801.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297801.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC
    CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT
    ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG
    CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT
    CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT
    GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG
    TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG
    ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG
    ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC
    AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT
    GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT
    TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG
    ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG
    CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG
    CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG
    AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC
    GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC
    AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT
    GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG
    GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG
    AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG
    TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG
    GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG
    GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT
    TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA
    CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG
    CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC
    GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT
    AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG
    CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT
    ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT
    CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA
    CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG
    GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG
    TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA
    CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA
    CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG
    CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC
    ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA
    AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG
    TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT
    CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT
    AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG
    GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC
    AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC
    CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG
    GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA
    CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCTGCT
    GGCTCCTATG CCAACATGCT AAACAAAATC ATTGCTGGTA TTTTAGGAGA CGGCCAGGAA
    GAGCCGGTGG ACAGCAGACC GGTCAGCTTC CCGTCTCTGC TCAGTCACAT GACCTCCACC
    ATCCTCAACC ATGAGGTGCT GGCACTGCCC GTGTTGCCCA GGAAACATGG CGTGCCACCC
    ATGCGAACCT TCGCCCCATT GGCTGGCACT CTGCCCTGCG ACTTCCACCT GCTGAACCTC
    AGGAGCATCC AAAGCCCGCA GGACGCTCAC TCTCCATCTC CCTATATGGC TTTGCTGCTG
    CACAGACTGG GTTTGGACTG TGGTCTGGAG CCGCAGAATC CTGGTTTCAA CTGCACCACC
    ACACAAGGCC AGCTCTCCGT TTCTGGGCTG TTCCGTGGGC TGGATCTCCA GCTCCTGCAG
    CCCATGTCTC TGTCTCTGAT GCACTCTCAG CCTCCGCTCT CTAACGACTC CTCCGTCAGT
    CTGGAGCCAA TGGAGATCTC CGCCTACAAG CTCAAGCTGT GCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49901
    Clone ID Related Accession (Same CDS sequence) XM_009297802.1 , XM_009297802.2
    Accession Version XM_009297802.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product alpha-mannosidase 2x isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAACCGCAT CGAGCAGCTG GAACAGCTCC TGGAGGAGAA CCATCAGATC
    ATCAGCCACA TTAAAGACTC AGTGCTAGAG CTCACTGATA CGGGCGCCAT CTCTCCCAGT
    GGACACCTGC CCTTCCGGAG CGCCAACGGC TCCTGGGTGC TGCCGTTTGA TGGACGGCCC
    ACGTTTCTCT CAGTCAAGCC ATTGGACTGC CAGTTTGCTC TGGGCCGCAG GAGCCAAACT
    GACCTGCAGA TGCTGGATGT TTATTCCTTG CTGAACTTTG ATAATGTGGA CGGAGGGGTC
    TGGAAGCAAG GCTTTGAGAT CACCTATGAG CCCAACGAGT GGGACGATGA GCCCTTGCAG
    GTGTTTGTGG TTCCGCATTC GCACAACGAT CCAGGATGGA TCAAAACGTT TGACAAGTAC
    TACAACGACC AGACCCAGCA CATCTTAAAC AACATGGTGA CGAAGATGGC AGAAGATCCT
    CGCCGGAAGT TCATCTGGTC GGAAATATCG TACTTCGCCA AATGGTGGGA AAGCGCAGAT
    GTGCATAAAC AAGAGGTTAT GCGCAAGCTA ATCCTCAGTG GACAGCTGGA GATGGTAACC
    GGAGGCTGGG TGATGACGGA CGAAGCCAAC GCTCACTATT TCGCCATGAT CGATCAGCTC
    ATTGAAGGCC ATCAGTGGCT GGAGAGGAAT CTGGGTGTGA CCCCTCGCTC GGGCTGGGCT
    GTTGATCCAT TCGGGCACAG CGCCACCATG CCTTACCTGC TGAAACGGGC CAACATAAGC
    AGCATGTTGA TCCAGAGGGT CCATTACTCC ATCAAGAAGC ACTTCGCAGC CACACGCAGC
    CTTGAGTTCA TGTGGAGACA GGCCTGGGAT ATCGAGTCGA GCACAGACAT GTTCTGCCAC
    ATGATGCCGT TTTACAGCTA TGATGTGCCT CACACGTGCG GCCCCGACCC CAAAATCTGC
    TGCCAGTTTG ATTTCAAGAG GCTGCCGGGC AGTCGAGTCA ACTGCCCGTG GAAGGTGCCG
    CCCAGAGCTA TCAATGATGC CAATGTGGCC GAGAGAGCTG GCGTTCTGCT CGATCAATAT
    CGTAAAAAAT CGAAGCTGTT CCGGAGCAAA GTGTTGCTGG TTCCTCTGGG GGACGACTTT
    CGCTATGATA AAGCTTTAGA GTGGGACCAG CAGTACTTGA ACTACCAGAA ACTGTTCGAT
    TACATGAACT CCCATCCAGA AATGCACGTG AAGGCTCAGT TTGCGACGCT GTCAGACTAC
    TTTGATGCGG TGTACAAGGC TAATGGAGTG ACTTCGGGGG CGAGACCACC CGATTACCCT
    GTGCTGAGCG GAGACTTCTT CGCTTATGCA GACAGAGAGG ACCATTACTG GAGTGGATAC
    TACACCTCGC GACCCTTCTA CAAAAACCTG GACCGCATTT TAGAATCTCA CCTAAGAGGG
    GCAGAAATCC TCTATAGTTT GGCGGTGGCT CATGCACGAC ATGCAGGAGT TGAAGGACGA
    TATCCGGTTT CAGATTACTC TCTCCTGACT GATGCCAGGC GAAACATTGG GCTGTTCCAG
    CATCATGATG CCATCACGGG CACTGCCAAA GAGGCTGTCG TCATCGACTA CGGGAACAGG
    CTGTTACGTT CTCTGGTGGG TCTGAAACGA GTGATCATTA ATGCAGCGCA CTTCCTGATA
    ATAAAGAATA AAGACATTTA TCGCTTCTAC CAGACAGAGC CCTTCTTGGA GACGGATGAT
    AGACGCGCCA CACAGGACTC TCTCCCGCAG CGTACGCTTA TCGAGCTGGA ATCTTCACCA
    AGGTATCTGG TGCTGTTTAA CCCGGTGGAG CAGGAGCGTC TGTGTTTGGT CACTGTCCTG
    GTGAATTCGG CAAGAGTGAG GGTGCTTACA GAAGACGGAC AAACGCTACC GGTGCAGCTC
    AGCGCGCAGT GGATGTCCGC TACGGAGATG AGTGGGGAGG TTTATCAGGC TTCTTTCATG
    GCACGTCTGC CGGCTCTGGG ATTGGCCGTC TTCCACCTGT ACGACTCTGC TGATTCGCCC
    ATGACTCTGC GCTCCGAGAC GCTGCTCAGG GTTCCAGGAC GGGGTCAGAG CATTCGGGGT
    TTGGATCCTC TGCCTGTGCG CTCACAAACT GTTGATGGAC AGCCGTTCTA CATCCAAAGC
    CAATCCCTCA CTCTGGGCTT CTCTGGAGCC ACAGGGCTGC TGGAGAGCAT TCGACGGAAA
    GACGATCCCC AGGAGGTGAG GGTACAGATT CAGTTCCTCA CCTACGGTAC GCGACCCTCC
    AAGGACAAGA GCGGGGCCTA TCTGTTCCTG CCGGATGGAA ATGCTAAGCC GTATTCGCAG
    AGAGAGGCCC CTATCGTGCG TGTGGTTGAA GGTCCTCTGT TCGCAGAGGT TGTGGCACAT
    TATCAGCACT TCCAGCAGAC GGTCCGCATT CATAATGTTC CAGGGGTTGA CGGTCTTTCT
    TTGGACATCA CCACTATGGT GGACATCAGA GATCAGAATA ACAAAGAGCT GGCCATGAGG
    TTCGTCACCG ACATCCAGAG CGGAGATACT TTCTACACGG ATCTCAACGG CTTTCAGATT
    CAGCCTCGGC GATACTTCCA GAAACTTCCA CTACAAGCCA ATTTCTACCC CATGCCCAGC
    ACAGCATACA TCCAGGACAG CCAGTACCGG CTCACGCTCC ACACGGCACA GGCTCTGGGG
    GTTACCAGTC TGGCCAGCGG TCAGCTGGAG GTAATCCTGG ACCGCAGACT GATGCAGGAT
    GATAACAGGG GATTGGGACA AGGCCTGAAA GACAACAAAC GGACAGCTAA CAGGTTCCGT
    CTTCTGCTGG AGAGGCGGAG CAACAGTGCA AAGCCTGCTG GCTCCTATGC CAACATGCTA
    AACAAAATCA TTGCTGGTAT TTTAGGAGAC GGCCAGGAAG AGCCGGTGGA CAGCAGACCG
    GTCAGCTTCC CGTCTCTGCT CAGTCACATG ACCTCCACCA TCCTCAACCA TGAGGTGCTG
    GCACTGCCCG TGTTGCCCAG GAAACATGGC GTGCCACCCA TGCGAACCTT CGCCCCATTG
    GCTGGCACTC TGCCCTGCGA CTTCCACCTG CTGAACCTCA GGAGCATCCA AAGCCCGCAG
    GACGCTCACT CTCCATCTCC CTATATGGCT TTGCTGCTGC ACAGACTGGG TTTGGACTGT
    GGTCTGGAGC CGCAGAATCC TGGTTTCAAC TGCACCACCA CACAAGGCCA GCTCTCCGTT
    TCTGGGCTGT TCCGTGGGCT GGATCTCCAG CTCCTGCAGC CCATGTCTCT GTCTCTGATG
    CACTCTCAGC CTCCGCTCTC TAACGACTCC TCCGTCAGTC TGGAGCCAAT GGAGATCTCC
    GCCTACAAGC TCAAGCTGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296077.1
    CDS788..4291
    Translation

    Target ORF information:

    RefSeq Version XM_009297802.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297802.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAACCGCAT CGAGCAGCTG GAACAGCTCC TGGAGGAGAA CCATCAGATC
    ATCAGCCACA TTAAAGACTC AGTGCTAGAG CTCACTGATA CGGGCGCCAT CTCTCCCAGT
    GGACACCTGC CCTTCCGGAG CGCCAACGGC TCCTGGGTGC TGCCGTTTGA TGGACGGCCC
    ACGTTTCTCT CAGTCAAGCC ATTGGACTGC CAGTTTGCTC TGGGCCGCAG GAGCCAAACT
    GACCTGCAGA TGCTGGATGT TTATTCCTTG CTGAACTTTG ATAATGTGGA CGGAGGGGTC
    TGGAAGCAAG GCTTTGAGAT CACCTATGAG CCCAACGAGT GGGACGATGA GCCCTTGCAG
    GTGTTTGTGG TTCCGCATTC GCACAACGAT CCAGGATGGA TCAAAACGTT TGACAAGTAC
    TACAACGACC AGACCCAGCA CATCTTAAAC AACATGGTGA CGAAGATGGC AGAAGATCCT
    CGCCGGAAGT TCATCTGGTC GGAAATATCG TACTTCGCCA AATGGTGGGA AAGCGCAGAT
    GTGCATAAAC AAGAGGTTAT GCGCAAGCTA ATCCTCAGTG GACAGCTGGA GATGGTAACC
    GGAGGCTGGG TGATGACGGA CGAAGCCAAC GCTCACTATT TCGCCATGAT CGATCAGCTC
    ATTGAAGGCC ATCAGTGGCT GGAGAGGAAT CTGGGTGTGA CCCCTCGCTC GGGCTGGGCT
    GTTGATCCAT TCGGGCACAG CGCCACCATG CCTTACCTGC TGAAACGGGC CAACATAAGC
    AGCATGTTGA TCCAGAGGGT CCATTACTCC ATCAAGAAGC ACTTCGCAGC CACACGCAGC
    CTTGAGTTCA TGTGGAGACA GGCCTGGGAT ATCGAGTCGA GCACAGACAT GTTCTGCCAC
    ATGATGCCGT TTTACAGCTA TGATGTGCCT CACACGTGCG GCCCCGACCC CAAAATCTGC
    TGCCAGTTTG ATTTCAAGAG GCTGCCGGGC AGTCGAGTCA ACTGCCCGTG GAAGGTGCCG
    CCCAGAGCTA TCAATGATGC CAATGTGGCC GAGAGAGCTG GCGTTCTGCT CGATCAATAT
    CGTAAAAAAT CGAAGCTGTT CCGGAGCAAA GTGTTGCTGG TTCCTCTGGG GGACGACTTT
    CGCTATGATA AAGCTTTAGA GTGGGACCAG CAGTACTTGA ACTACCAGAA ACTGTTCGAT
    TACATGAACT CCCATCCAGA AATGCACGTG AAGGCTCAGT TTGCGACGCT GTCAGACTAC
    TTTGATGCGG TGTACAAGGC TAATGGAGTG ACTTCGGGGG CGAGACCACC CGATTACCCT
    GTGCTGAGCG GAGACTTCTT CGCTTATGCA GACAGAGAGG ACCATTACTG GAGTGGATAC
    TACACCTCGC GACCCTTCTA CAAAAACCTG GACCGCATTT TAGAATCTCA CCTAAGAGGG
    GCAGAAATCC TCTATAGTTT GGCGGTGGCT CATGCACGAC ATGCAGGAGT TGAAGGACGA
    TATCCGGTTT CAGATTACTC TCTCCTGACT GATGCCAGGC GAAACATTGG GCTGTTCCAG
    CATCATGATG CCATCACGGG CACTGCCAAA GAGGCTGTCG TCATCGACTA CGGGAACAGG
    CTGTTACGTT CTCTGGTGGG TCTGAAACGA GTGATCATTA ATGCAGCGCA CTTCCTGATA
    ATAAAGAATA AAGACATTTA TCGCTTCTAC CAGACAGAGC CCTTCTTGGA GACGGATGAT
    AGACGCGCCA CACAGGACTC TCTCCCGCAG CGTACGCTTA TCGAGCTGGA ATCTTCACCA
    AGGTATCTGG TGCTGTTTAA CCCGGTGGAG CAGGAGCGTC TGTGTTTGGT CACTGTCCTG
    GTGAATTCGG CAAGAGTGAG GGTGCTTACA GAAGACGGAC AAACGCTACC GGTGCAGCTC
    AGCGCGCAGT GGATGTCCGC TACGGAGATG AGTGGGGAGG TTTATCAGGC TTCTTTCATG
    GCACGTCTGC CGGCTCTGGG ATTGGCCGTC TTCCACCTGT ACGACTCTGC TGATTCGCCC
    ATGACTCTGC GCTCCGAGAC GCTGCTCAGG GTTCCAGGAC GGGGTCAGAG CATTCGGGGT
    TTGGATCCTC TGCCTGTGCG CTCACAAACT GTTGATGGAC AGCCGTTCTA CATCCAAAGC
    CAATCCCTCA CTCTGGGCTT CTCTGGAGCC ACAGGGCTGC TGGAGAGCAT TCGACGGAAA
    GACGATCCCC AGGAGGTGAG GGTACAGATT CAGTTCCTCA CCTACGGTAC GCGACCCTCC
    AAGGACAAGA GCGGGGCCTA TCTGTTCCTG CCGGATGGAA ATGCTAAGCC GTATTCGCAG
    AGAGAGGCCC CTATCGTGCG TGTGGTTGAA GGTCCTCTGT TCGCAGAGGT TGTGGCACAT
    TATCAGCACT TCCAGCAGAC GGTCCGCATT CATAATGTTC CAGGGGTTGA CGGTCTTTCT
    TTGGACATCA CCACTATGGT GGACATCAGA GATCAGAATA ACAAAGAGCT GGCCATGAGG
    TTCGTCACCG ACATCCAGAG CGGAGATACT TTCTACACGG ATCTCAACGG CTTTCAGATT
    CAGCCTCGGC GATACTTCCA GAAACTTCCA CTACAAGCCA ATTTCTACCC CATGCCCAGC
    ACAGCATACA TCCAGGACAG CCAGTACCGG CTCACGCTCC ACACGGCACA GGCTCTGGGG
    GTTACCAGTC TGGCCAGCGG TCAGCTGGAG GTAATCCTGG ACCGCAGACT GATGCAGGAT
    GATAACAGGG GATTGGGACA AGGCCTGAAA GACAACAAAC GGACAGCTAA CAGGTTCCGT
    CTTCTGCTGG AGAGGCGGAG CAACAGTGCA AAGCCTGCTG GCTCCTATGC CAACATGCTA
    AACAAAATCA TTGCTGGTAT TTTAGGAGAC GGCCAGGAAG AGCCGGTGGA CAGCAGACCG
    GTCAGCTTCC CGTCTCTGCT CAGTCACATG ACCTCCACCA TCCTCAACCA TGAGGTGCTG
    GCACTGCCCG TGTTGCCCAG GAAACATGGC GTGCCACCCA TGCGAACCTT CGCCCCATTG
    GCTGGCACTC TGCCCTGCGA CTTCCACCTG CTGAACCTCA GGAGCATCCA AAGCCCGCAG
    GACGCTCACT CTCCATCTCC CTATATGGCT TTGCTGCTGC ACAGACTGGG TTTGGACTGT
    GGTCTGGAGC CGCAGAATCC TGGTTTCAAC TGCACCACCA CACAAGGCCA GCTCTCCGTT
    TCTGGGCTGT TCCGTGGGCT GGATCTCCAG CTCCTGCAGC CCATGTCTCT GTCTCTGATG
    CACTCTCAGC CTCCGCTCTC TAACGACTCC TCCGTCAGTC TGGAGCCAAT GGAGATCTCC
    GCCTACAAGC TCAAGCTGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49901
    Clone ID Related Accession (Same CDS sequence) XM_009297802.1 , XM_009297802.2
    Accession Version XM_009297802.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3504bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alpha-mannosidase 2x isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297802.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAACCGCAT CGAGCAGCTG GAACAGCTCC TGGAGGAGAA CCATCAGATC
    ATCAGCCACA TTAAAGACTC AGTGCTAGAG CTCACTGATA CGGGCGCCAT CTCTCCCAGT
    GGACACCTGC CCTTCCGGAG CGCCAACGGC TCCTGGGTGC TGCCGTTTGA TGGACGGCCC
    ACGTTTCTCT CAGTCAAGCC ATTGGACTGC CAGTTTGCTC TGGGCCGCAG GAGCCAAACT
    GACCTGCAGA TGCTGGATGT TTATTCCTTG CTGAACTTTG ATAATGTGGA CGGAGGGGTC
    TGGAAGCAAG GCTTTGAGAT CACCTATGAG CCCAACGAGT GGGACGATGA GCCCTTGCAG
    GTGTTTGTGG TTCCGCATTC GCACAACGAT CCAGGATGGA TCAAAACGTT TGACAAGTAC
    TACAACGACC AGACCCAGCA CATCTTAAAC AACATGGTGA CGAAGATGGC AGAAGATCCT
    CGCCGGAAGT TCATCTGGTC GGAAATATCG TACTTCGCCA AATGGTGGGA AAGCGCAGAT
    GTGCATAAAC AAGAGGTTAT GCGCAAGCTA ATCCTCAGTG GACAGCTGGA GATGGTAACC
    GGAGGCTGGG TGATGACGGA CGAAGCCAAC GCTCACTATT TCGCCATGAT CGATCAGCTC
    ATTGAAGGCC ATCAGTGGCT GGAGAGGAAT CTGGGTGTGA CCCCTCGCTC GGGCTGGGCT
    GTTGATCCAT TCGGGCACAG CGCCACCATG CCTTACCTGC TGAAACGGGC CAACATAAGC
    AGCATGTTGA TCCAGAGGGT CCATTACTCC ATCAAGAAGC ACTTCGCAGC CACACGCAGC
    CTTGAGTTCA TGTGGAGACA GGCCTGGGAT ATCGAGTCGA GCACAGACAT GTTCTGCCAC
    ATGATGCCGT TTTACAGCTA TGATGTGCCT CACACGTGCG GCCCCGACCC CAAAATCTGC
    TGCCAGTTTG ATTTCAAGAG GCTGCCGGGC AGTCGAGTCA ACTGCCCGTG GAAGGTGCCG
    CCCAGAGCTA TCAATGATGC CAATGTGGCC GAGAGAGCTG GCGTTCTGCT CGATCAATAT
    CGTAAAAAAT CGAAGCTGTT CCGGAGCAAA GTGTTGCTGG TTCCTCTGGG GGACGACTTT
    CGCTATGATA AAGCTTTAGA GTGGGACCAG CAGTACTTGA ACTACCAGAA ACTGTTCGAT
    TACATGAACT CCCATCCAGA AATGCACGTG AAGGCTCAGT TTGCGACGCT GTCAGACTAC
    TTTGATGCGG TGTACAAGGC TAATGGAGTG ACTTCGGGGG CGAGACCACC CGATTACCCT
    GTGCTGAGCG GAGACTTCTT CGCTTATGCA GACAGAGAGG ACCATTACTG GAGTGGATAC
    TACACCTCGC GACCCTTCTA CAAAAACCTG GACCGCATTT TAGAATCTCA CCTAAGAGGG
    GCAGAAATCC TCTATAGTTT GGCGGTGGCT CATGCACGAC ATGCAGGAGT TGAAGGACGA
    TATCCGGTTT CAGATTACTC TCTCCTGACT GATGCCAGGC GAAACATTGG GCTGTTCCAG
    CATCATGATG CCATCACGGG CACTGCCAAA GAGGCTGTCG TCATCGACTA CGGGAACAGG
    CTGTTACGTT CTCTGGTGGG TCTGAAACGA GTGATCATTA ATGCAGCGCA CTTCCTGATA
    ATAAAGAATA AAGACATTTA TCGCTTCTAC CAGACAGAGC CCTTCTTGGA GACGGATGAT
    AGACGCGCCA CACAGGACTC TCTCCCGCAG CGTACGCTTA TCGAGCTGGA ATCTTCACCA
    AGGTATCTGG TGCTGTTTAA CCCGGTGGAG CAGGAGCGTC TGTGTTTGGT CACTGTCCTG
    GTGAATTCGG CAAGAGTGAG GGTGCTTACA GAAGACGGAC AAACGCTACC GGTGCAGCTC
    AGCGCGCAGT GGATGTCCGC TACGGAGATG AGTGGGGAGG TTTATCAGGC TTCTTTCATG
    GCACGTCTGC CGGCTCTGGG ATTGGCCGTC TTCCACCTGT ACGACTCTGC TGATTCGCCC
    ATGACTCTGC GCTCCGAGAC GCTGCTCAGG GTTCCAGGAC GGGGTCAGAG CATTCGGGGT
    TTGGATCCTC TGCCTGTGCG CTCACAAACT GTTGATGGAC AGCCGTTCTA CATCCAAAGC
    CAATCCCTCA CTCTGGGCTT CTCTGGAGCC ACAGGGCTGC TGGAGAGCAT TCGACGGAAA
    GACGATCCCC AGGAGGTGAG GGTACAGATT CAGTTCCTCA CCTACGGTAC GCGACCCTCC
    AAGGACAAGA GCGGGGCCTA TCTGTTCCTG CCGGATGGAA ATGCTAAGCC GTATTCGCAG
    AGAGAGGCCC CTATCGTGCG TGTGGTTGAA GGTCCTCTGT TCGCAGAGGT TGTGGCACAT
    TATCAGCACT TCCAGCAGAC GGTCCGCATT CATAATGTTC CAGGGGTTGA CGGTCTTTCT
    TTGGACATCA CCACTATGGT GGACATCAGA GATCAGAATA ACAAAGAGCT GGCCATGAGG
    TTCGTCACCG ACATCCAGAG CGGAGATACT TTCTACACGG ATCTCAACGG CTTTCAGATT
    CAGCCTCGGC GATACTTCCA GAAACTTCCA CTACAAGCCA ATTTCTACCC CATGCCCAGC
    ACAGCATACA TCCAGGACAG CCAGTACCGG CTCACGCTCC ACACGGCACA GGCTCTGGGG
    GTTACCAGTC TGGCCAGCGG TCAGCTGGAG GTAATCCTGG ACCGCAGACT GATGCAGGAT
    GATAACAGGG GATTGGGACA AGGCCTGAAA GACAACAAAC GGACAGCTAA CAGGTTCCGT
    CTTCTGCTGG AGAGGCGGAG CAACAGTGCA AAGCCTGCTG GCTCCTATGC CAACATGCTA
    AACAAAATCA TTGCTGGTAT TTTAGGAGAC GGCCAGGAAG AGCCGGTGGA CAGCAGACCG
    GTCAGCTTCC CGTCTCTGCT CAGTCACATG ACCTCCACCA TCCTCAACCA TGAGGTGCTG
    GCACTGCCCG TGTTGCCCAG GAAACATGGC GTGCCACCCA TGCGAACCTT CGCCCCATTG
    GCTGGCACTC TGCCCTGCGA CTTCCACCTG CTGAACCTCA GGAGCATCCA AAGCCCGCAG
    GACGCTCACT CTCCATCTCC CTATATGGCT TTGCTGCTGC ACAGACTGGG TTTGGACTGT
    GGTCTGGAGC CGCAGAATCC TGGTTTCAAC TGCACCACCA CACAAGGCCA GCTCTCCGTT
    TCTGGGCTGT TCCGTGGGCT GGATCTCCAG CTCCTGCAGC CCATGTCTCT GTCTCTGATG
    CACTCTCAGC CTCCGCTCTC TAACGACTCC TCCGTCAGTC TGGAGCCAAT GGAGATCTCC
    GCCTACAAGC TCAAGCTGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296077.1
    CDS788..4291
    Translation

    Target ORF information:

    RefSeq Version XM_009297802.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297802.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAACCGCAT CGAGCAGCTG GAACAGCTCC TGGAGGAGAA CCATCAGATC
    ATCAGCCACA TTAAAGACTC AGTGCTAGAG CTCACTGATA CGGGCGCCAT CTCTCCCAGT
    GGACACCTGC CCTTCCGGAG CGCCAACGGC TCCTGGGTGC TGCCGTTTGA TGGACGGCCC
    ACGTTTCTCT CAGTCAAGCC ATTGGACTGC CAGTTTGCTC TGGGCCGCAG GAGCCAAACT
    GACCTGCAGA TGCTGGATGT TTATTCCTTG CTGAACTTTG ATAATGTGGA CGGAGGGGTC
    TGGAAGCAAG GCTTTGAGAT CACCTATGAG CCCAACGAGT GGGACGATGA GCCCTTGCAG
    GTGTTTGTGG TTCCGCATTC GCACAACGAT CCAGGATGGA TCAAAACGTT TGACAAGTAC
    TACAACGACC AGACCCAGCA CATCTTAAAC AACATGGTGA CGAAGATGGC AGAAGATCCT
    CGCCGGAAGT TCATCTGGTC GGAAATATCG TACTTCGCCA AATGGTGGGA AAGCGCAGAT
    GTGCATAAAC AAGAGGTTAT GCGCAAGCTA ATCCTCAGTG GACAGCTGGA GATGGTAACC
    GGAGGCTGGG TGATGACGGA CGAAGCCAAC GCTCACTATT TCGCCATGAT CGATCAGCTC
    ATTGAAGGCC ATCAGTGGCT GGAGAGGAAT CTGGGTGTGA CCCCTCGCTC GGGCTGGGCT
    GTTGATCCAT TCGGGCACAG CGCCACCATG CCTTACCTGC TGAAACGGGC CAACATAAGC
    AGCATGTTGA TCCAGAGGGT CCATTACTCC ATCAAGAAGC ACTTCGCAGC CACACGCAGC
    CTTGAGTTCA TGTGGAGACA GGCCTGGGAT ATCGAGTCGA GCACAGACAT GTTCTGCCAC
    ATGATGCCGT TTTACAGCTA TGATGTGCCT CACACGTGCG GCCCCGACCC CAAAATCTGC
    TGCCAGTTTG ATTTCAAGAG GCTGCCGGGC AGTCGAGTCA ACTGCCCGTG GAAGGTGCCG
    CCCAGAGCTA TCAATGATGC CAATGTGGCC GAGAGAGCTG GCGTTCTGCT CGATCAATAT
    CGTAAAAAAT CGAAGCTGTT CCGGAGCAAA GTGTTGCTGG TTCCTCTGGG GGACGACTTT
    CGCTATGATA AAGCTTTAGA GTGGGACCAG CAGTACTTGA ACTACCAGAA ACTGTTCGAT
    TACATGAACT CCCATCCAGA AATGCACGTG AAGGCTCAGT TTGCGACGCT GTCAGACTAC
    TTTGATGCGG TGTACAAGGC TAATGGAGTG ACTTCGGGGG CGAGACCACC CGATTACCCT
    GTGCTGAGCG GAGACTTCTT CGCTTATGCA GACAGAGAGG ACCATTACTG GAGTGGATAC
    TACACCTCGC GACCCTTCTA CAAAAACCTG GACCGCATTT TAGAATCTCA CCTAAGAGGG
    GCAGAAATCC TCTATAGTTT GGCGGTGGCT CATGCACGAC ATGCAGGAGT TGAAGGACGA
    TATCCGGTTT CAGATTACTC TCTCCTGACT GATGCCAGGC GAAACATTGG GCTGTTCCAG
    CATCATGATG CCATCACGGG CACTGCCAAA GAGGCTGTCG TCATCGACTA CGGGAACAGG
    CTGTTACGTT CTCTGGTGGG TCTGAAACGA GTGATCATTA ATGCAGCGCA CTTCCTGATA
    ATAAAGAATA AAGACATTTA TCGCTTCTAC CAGACAGAGC CCTTCTTGGA GACGGATGAT
    AGACGCGCCA CACAGGACTC TCTCCCGCAG CGTACGCTTA TCGAGCTGGA ATCTTCACCA
    AGGTATCTGG TGCTGTTTAA CCCGGTGGAG CAGGAGCGTC TGTGTTTGGT CACTGTCCTG
    GTGAATTCGG CAAGAGTGAG GGTGCTTACA GAAGACGGAC AAACGCTACC GGTGCAGCTC
    AGCGCGCAGT GGATGTCCGC TACGGAGATG AGTGGGGAGG TTTATCAGGC TTCTTTCATG
    GCACGTCTGC CGGCTCTGGG ATTGGCCGTC TTCCACCTGT ACGACTCTGC TGATTCGCCC
    ATGACTCTGC GCTCCGAGAC GCTGCTCAGG GTTCCAGGAC GGGGTCAGAG CATTCGGGGT
    TTGGATCCTC TGCCTGTGCG CTCACAAACT GTTGATGGAC AGCCGTTCTA CATCCAAAGC
    CAATCCCTCA CTCTGGGCTT CTCTGGAGCC ACAGGGCTGC TGGAGAGCAT TCGACGGAAA
    GACGATCCCC AGGAGGTGAG GGTACAGATT CAGTTCCTCA CCTACGGTAC GCGACCCTCC
    AAGGACAAGA GCGGGGCCTA TCTGTTCCTG CCGGATGGAA ATGCTAAGCC GTATTCGCAG
    AGAGAGGCCC CTATCGTGCG TGTGGTTGAA GGTCCTCTGT TCGCAGAGGT TGTGGCACAT
    TATCAGCACT TCCAGCAGAC GGTCCGCATT CATAATGTTC CAGGGGTTGA CGGTCTTTCT
    TTGGACATCA CCACTATGGT GGACATCAGA GATCAGAATA ACAAAGAGCT GGCCATGAGG
    TTCGTCACCG ACATCCAGAG CGGAGATACT TTCTACACGG ATCTCAACGG CTTTCAGATT
    CAGCCTCGGC GATACTTCCA GAAACTTCCA CTACAAGCCA ATTTCTACCC CATGCCCAGC
    ACAGCATACA TCCAGGACAG CCAGTACCGG CTCACGCTCC ACACGGCACA GGCTCTGGGG
    GTTACCAGTC TGGCCAGCGG TCAGCTGGAG GTAATCCTGG ACCGCAGACT GATGCAGGAT
    GATAACAGGG GATTGGGACA AGGCCTGAAA GACAACAAAC GGACAGCTAA CAGGTTCCGT
    CTTCTGCTGG AGAGGCGGAG CAACAGTGCA AAGCCTGCTG GCTCCTATGC CAACATGCTA
    AACAAAATCA TTGCTGGTAT TTTAGGAGAC GGCCAGGAAG AGCCGGTGGA CAGCAGACCG
    GTCAGCTTCC CGTCTCTGCT CAGTCACATG ACCTCCACCA TCCTCAACCA TGAGGTGCTG
    GCACTGCCCG TGTTGCCCAG GAAACATGGC GTGCCACCCA TGCGAACCTT CGCCCCATTG
    GCTGGCACTC TGCCCTGCGA CTTCCACCTG CTGAACCTCA GGAGCATCCA AAGCCCGCAG
    GACGCTCACT CTCCATCTCC CTATATGGCT TTGCTGCTGC ACAGACTGGG TTTGGACTGT
    GGTCTGGAGC CGCAGAATCC TGGTTTCAAC TGCACCACCA CACAAGGCCA GCTCTCCGTT
    TCTGGGCTGT TCCGTGGGCT GGATCTCCAG CTCCTGCAGC CCATGTCTCT GTCTCTGATG
    CACTCTCAGC CTCCGCTCTC TAACGACTCC TCCGTCAGTC TGGAGCCAAT GGAGATCTCC
    GCCTACAAGC TCAAGCTGTG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49902
    Clone ID Related Accession (Same CDS sequence) XM_009297803.1 , XM_009297803.2
    Accession Version XM_009297803.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product alpha-mannosidase 2x isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC
    CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT
    ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG
    CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT
    CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT
    GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG
    TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG
    ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG
    ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC
    AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT
    GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT
    TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG
    ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG
    CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG
    CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG
    AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC
    GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC
    AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT
    GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG
    GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG
    AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG
    TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG
    GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG
    GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT
    TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA
    CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG
    CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC
    GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT
    AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG
    CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT
    ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT
    CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA
    CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG
    GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG
    TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA
    CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA
    CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG
    CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC
    ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA
    AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG
    TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT
    CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT
    AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG
    GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC
    AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC
    CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG
    GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA
    CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCGGTG
    GACAGCAGAC CGGTCAGCTT CCCGTCTCTG CTCAGTCACA TGACCTCCAC CATCCTCAAC
    CATGAGGTGC TGGCACTGCC CGTGTTGCCC AGGAAACATG GCGTGCCACC CATGCGAACC
    TTCGCCCCAT TGGCTGGCAC TCTGCCCTGC GACTTCCACC TGCTGAACCT CAGGAGCATC
    CAAAGCCCGC AGGACGCTCA CTCTCCATCT CCCTATATGG CTTTGCTGCT GCACAGACTG
    GGTTTGGACT GTGGTCTGGA GCCGCAGAAT CCTGGTTTCA ACTGCACCAC CACACAAGGC
    CAGCTCTCCG TTTCTGGGCT GTTCCGTGGG CTGGATCTCC AGCTCCTGCA GCCCATGTCT
    CTGTCTCTGA TGCACTCTCA GCCTCCGCTC TCTAACGACT CCTCCGTCAG TCTGGAGCCA
    ATGGAGATCT CCGCCTACAA GCTCAAGCTG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296078.1
    CDS788..4243
    Translation

    Target ORF information:

    RefSeq Version XM_009297803.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297803.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC
    CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT
    ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG
    CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT
    CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT
    GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG
    TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG
    ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG
    ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC
    AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT
    GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT
    TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG
    ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG
    CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG
    CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG
    AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC
    GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC
    AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT
    GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG
    GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG
    AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG
    TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG
    GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG
    GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT
    TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA
    CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG
    CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC
    GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT
    AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG
    CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT
    ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT
    CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA
    CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG
    GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG
    TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA
    CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA
    CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG
    CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC
    ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA
    AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG
    TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT
    CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT
    AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG
    GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC
    AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC
    CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG
    GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA
    CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCGGTG
    GACAGCAGAC CGGTCAGCTT CCCGTCTCTG CTCAGTCACA TGACCTCCAC CATCCTCAAC
    CATGAGGTGC TGGCACTGCC CGTGTTGCCC AGGAAACATG GCGTGCCACC CATGCGAACC
    TTCGCCCCAT TGGCTGGCAC TCTGCCCTGC GACTTCCACC TGCTGAACCT CAGGAGCATC
    CAAAGCCCGC AGGACGCTCA CTCTCCATCT CCCTATATGG CTTTGCTGCT GCACAGACTG
    GGTTTGGACT GTGGTCTGGA GCCGCAGAAT CCTGGTTTCA ACTGCACCAC CACACAAGGC
    CAGCTCTCCG TTTCTGGGCT GTTCCGTGGG CTGGATCTCC AGCTCCTGCA GCCCATGTCT
    CTGTCTCTGA TGCACTCTCA GCCTCCGCTC TCTAACGACT CCTCCGTCAG TCTGGAGCCA
    ATGGAGATCT CCGCCTACAA GCTCAAGCTG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49902
    Clone ID Related Accession (Same CDS sequence) XM_009297803.1 , XM_009297803.2
    Accession Version XM_009297803.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3456bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product alpha-mannosidase 2x isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297803.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC
    CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT
    ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG
    CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT
    CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT
    GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG
    TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG
    ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG
    ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC
    AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT
    GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT
    TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG
    ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG
    CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG
    CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG
    AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC
    GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC
    AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT
    GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG
    GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG
    AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG
    TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG
    GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG
    GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT
    TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA
    CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG
    CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC
    GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT
    AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG
    CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT
    ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT
    CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA
    CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG
    GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG
    TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA
    CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA
    CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG
    CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC
    ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA
    AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG
    TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT
    CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT
    AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG
    GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC
    AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC
    CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG
    GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA
    CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCGGTG
    GACAGCAGAC CGGTCAGCTT CCCGTCTCTG CTCAGTCACA TGACCTCCAC CATCCTCAAC
    CATGAGGTGC TGGCACTGCC CGTGTTGCCC AGGAAACATG GCGTGCCACC CATGCGAACC
    TTCGCCCCAT TGGCTGGCAC TCTGCCCTGC GACTTCCACC TGCTGAACCT CAGGAGCATC
    CAAAGCCCGC AGGACGCTCA CTCTCCATCT CCCTATATGG CTTTGCTGCT GCACAGACTG
    GGTTTGGACT GTGGTCTGGA GCCGCAGAAT CCTGGTTTCA ACTGCACCAC CACACAAGGC
    CAGCTCTCCG TTTCTGGGCT GTTCCGTGGG CTGGATCTCC AGCTCCTGCA GCCCATGTCT
    CTGTCTCTGA TGCACTCTCA GCCTCCGCTC TCTAACGACT CCTCCGTCAG TCTGGAGCCA
    ATGGAGATCT CCGCCTACAA GCTCAAGCTG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009296078.1
    CDS788..4243
    Translation

    Target ORF information:

    RefSeq Version XM_009297803.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio mannosidase, alpha, class 2A, member 2 (man2a2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009297803.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGAAGCTGA AGAAGCAGGT GACAGTCTGC GGAGGGGCCA TCTTCTGTGT GGCCGTCTTC 
    TCCCTCTATC TGATGTTGGA CCGCGTCCAG CACGATCCGG CCAGGAGACA GAACGGCGGG
    AACTTTCCGA GGAGCCAGAT TTCGGTGTTG CAGAACCGCA TCGAGCAGCT GGAACAGCTC
    CTGGAGGAGA ACCATCAGAT CATCAGCCAC ATTAAAGACT CAGTGCTAGA GCTCACTGAT
    ACGGGCGCCA TCTCTCCCAG TGGACACCTG CCCTTCCGGA GCGCCAACGG CTCCTGGGTG
    CTGCCGTTTG ATGGACGGCC CACGTTTCTC TCAGTCAAGC CATTGGACTG CCAGTTTGCT
    CTGGGCCGCA GGAGCCAAAC TGACCTGCAG ATGCTGGATG TTTATTCCTT GCTGAACTTT
    GATAATGTGG ACGGAGGGGT CTGGAAGCAA GGCTTTGAGA TCACCTATGA GCCCAACGAG
    TGGGACGATG AGCCCTTGCA GGTGTTTGTG GTTCCGCATT CGCACAACGA TCCAGGATGG
    ATCAAAACGT TTGACAAGTA CTACAACGAC CAGACCCAGC ACATCTTAAA CAACATGGTG
    ACGAAGATGG CAGAAGATCC TCGCCGGAAG TTCATCTGGT CGGAAATATC GTACTTCGCC
    AAATGGTGGG AAAGCGCAGA TGTGCATAAA CAAGAGGTTA TGCGCAAGCT AATCCTCAGT
    GGACAGCTGG AGATGGTAAC CGGAGGCTGG GTGATGACGG ACGAAGCCAA CGCTCACTAT
    TTCGCCATGA TCGATCAGCT CATTGAAGGC CATCAGTGGC TGGAGAGGAA TCTGGGTGTG
    ACCCCTCGCT CGGGCTGGGC TGTTGATCCA TTCGGGCACA GCGCCACCAT GCCTTACCTG
    CTGAAACGGG CCAACATAAG CAGCATGTTG ATCCAGAGGG TCCATTACTC CATCAAGAAG
    CACTTCGCAG CCACACGCAG CCTTGAGTTC ATGTGGAGAC AGGCCTGGGA TATCGAGTCG
    AGCACAGACA TGTTCTGCCA CATGATGCCG TTTTACAGCT ATGATGTGCC TCACACGTGC
    GGCCCCGACC CCAAAATCTG CTGCCAGTTT GATTTCAAGA GGCTGCCGGG CAGTCGAGTC
    AACTGCCCGT GGAAGGTGCC GCCCAGAGCT ATCAATGATG CCAATGTGGC CGAGAGAGCT
    GGCGTTCTGC TCGATCAATA TCGTAAAAAA TCGAAGCTGT TCCGGAGCAA AGTGTTGCTG
    GTTCCTCTGG GGGACGACTT TCGCTATGAT AAAGCTTTAG AGTGGGACCA GCAGTACTTG
    AACTACCAGA AACTGTTCGA TTACATGAAC TCCCATCCAG AAATGCACGT GAAGGCTCAG
    TTTGCGACGC TGTCAGACTA CTTTGATGCG GTGTACAAGG CTAATGGAGT GACTTCGGGG
    GCGAGACCAC CCGATTACCC TGTGCTGAGC GGAGACTTCT TCGCTTATGC AGACAGAGAG
    GACCATTACT GGAGTGGATA CTACACCTCG CGACCCTTCT ACAAAAACCT GGACCGCATT
    TTAGAATCTC ACCTAAGAGG GGCAGAAATC CTCTATAGTT TGGCGGTGGC TCATGCACGA
    CATGCAGGAG TTGAAGGACG ATATCCGGTT TCAGATTACT CTCTCCTGAC TGATGCCAGG
    CGAAACATTG GGCTGTTCCA GCATCATGAT GCCATCACGG GCACTGCCAA AGAGGCTGTC
    GTCATCGACT ACGGGAACAG GCTGTTACGT TCTCTGGTGG GTCTGAAACG AGTGATCATT
    AATGCAGCGC ACTTCCTGAT AATAAAGAAT AAAGACATTT ATCGCTTCTA CCAGACAGAG
    CCCTTCTTGG AGACGGATGA TAGACGCGCC ACACAGGACT CTCTCCCGCA GCGTACGCTT
    ATCGAGCTGG AATCTTCACC AAGGTATCTG GTGCTGTTTA ACCCGGTGGA GCAGGAGCGT
    CTGTGTTTGG TCACTGTCCT GGTGAATTCG GCAAGAGTGA GGGTGCTTAC AGAAGACGGA
    CAAACGCTAC CGGTGCAGCT CAGCGCGCAG TGGATGTCCG CTACGGAGAT GAGTGGGGAG
    GTTTATCAGG CTTCTTTCAT GGCACGTCTG CCGGCTCTGG GATTGGCCGT CTTCCACCTG
    TACGACTCTG CTGATTCGCC CATGACTCTG CGCTCCGAGA CGCTGCTCAG GGTTCCAGGA
    CGGGGTCAGA GCATTCGGGG TTTGGATCCT CTGCCTGTGC GCTCACAAAC TGTTGATGGA
    CAGCCGTTCT ACATCCAAAG CCAATCCCTC ACTCTGGGCT TCTCTGGAGC CACAGGGCTG
    CTGGAGAGCA TTCGACGGAA AGACGATCCC CAGGAGGTGA GGGTACAGAT TCAGTTCCTC
    ACCTACGGTA CGCGACCCTC CAAGGACAAG AGCGGGGCCT ATCTGTTCCT GCCGGATGGA
    AATGCTAAGC CGTATTCGCA GAGAGAGGCC CCTATCGTGC GTGTGGTTGA AGGTCCTCTG
    TTCGCAGAGG TTGTGGCACA TTATCAGCAC TTCCAGCAGA CGGTCCGCAT TCATAATGTT
    CCAGGGGTTG ACGGTCTTTC TTTGGACATC ACCACTATGG TGGACATCAG AGATCAGAAT
    AACAAAGAGC TGGCCATGAG GTTCGTCACC GACATCCAGA GCGGAGATAC TTTCTACACG
    GATCTCAACG GCTTTCAGAT TCAGCCTCGG CGATACTTCC AGAAACTTCC ACTACAAGCC
    AATTTCTACC CCATGCCCAG CACAGCATAC ATCCAGGACA GCCAGTACCG GCTCACGCTC
    CACACGGCAC AGGCTCTGGG GGTTACCAGT CTGGCCAGCG GTCAGCTGGA GGTAATCCTG
    GACCGCAGAC TGATGCAGGA TGATAACAGG GGATTGGGAC AAGGCCTGAA AGACAACAAA
    CGGACAGCTA ACAGGTTCCG TCTTCTGCTG GAGAGGCGGA GCAACAGTGC AAAGCCGGTG
    GACAGCAGAC CGGTCAGCTT CCCGTCTCTG CTCAGTCACA TGACCTCCAC CATCCTCAAC
    CATGAGGTGC TGGCACTGCC CGTGTTGCCC AGGAAACATG GCGTGCCACC CATGCGAACC
    TTCGCCCCAT TGGCTGGCAC TCTGCCCTGC GACTTCCACC TGCTGAACCT CAGGAGCATC
    CAAAGCCCGC AGGACGCTCA CTCTCCATCT CCCTATATGG CTTTGCTGCT GCACAGACTG
    GGTTTGGACT GTGGTCTGGA GCCGCAGAAT CCTGGTTTCA ACTGCACCAC CACACAAGGC
    CAGCTCTCCG TTTCTGGGCT GTTCCGTGGG CTGGATCTCC AGCTCCTGCA GCCCATGTCT
    CTGTCTCTGA TGCACTCTCA GCCTCCGCTC TCTAACGACT CCTCCGTCAG TCTGGAGCCA
    ATGGAGATCT CCGCCTACAA GCTCAAGCTG TGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.