SI:DKEY-222H21.10 cDNA ORF clone, Danio rerio(Zebrafish)

The following SI:DKEY-222H21.10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SI:DKEY-222H21.10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa53960 XM_017356850.2
Latest version!
Danio rerio si:dkey-222h21.10 (si:dkey-222h21.10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa53959 XM_017356849.1
Latest version!
Danio rerio si:dkey-222h21.10 (si:dkey-222h21.10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
ODa53959 XM_017356848.2
Latest version!
Danio rerio si:dkey-222h21.10 (si:dkey-222h21.10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
ODa53960 XM_017356850.1 Danio rerio si:dkey-222h21.10 (si:dkey-222h21.10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa53959 XM_017356848.1 Danio rerio si:dkey-222h21.10 (si:dkey-222h21.10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa53960
    Clone ID Related Accession (Same CDS sequence) XM_017356850.2 , XM_017356850.1
    Accession Version XM_017356850.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 3-like isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGTGCTC CGTCAAGTCA TGATCATCCT TCCACCTGTG ATGAAAACAT GACCTCTGAC 
    CCCAGGGTAA CTCTGAGAAA AACACCAAGA TTAGAGTCTT CTGGACCCAG TGGTGTGTCT
    GTGAAGAGTG ACTGGTCCAT GGAGCGACCT CCTGCATTGA GCAATCAACC AGTGACCTCT
    GATCCTCGTG TAAATCAGAG AAAGACACCA AGGTTGGAGT CTCCAGAACT CAGTGGTGTG
    TCTGTGAAGA GTGACTGGTC TAAAGAGCGA CCTCCTGCAT TCAGTGATGA ACCAGTGACC
    TCTGACCCTC GGAGGTGTGA TATACATGAG CGATGTGTGA ACATTACACA GAGCAACATT
    GGATCACAAA CACTGAAGAC AAGAGACCTA CAGAGAGTCA AAGACCAGCT CAAAGCCAGC
    ATGAAGAACA AGTATGAGCT GTTATCTGAA GGAATTAAAG TAAAAGAGAA TCAAACCCAC
    CTGAACCGTA TCTACACCCA GCTGTACATC ATAGAAGGAG AGAGTGAAGG AGTGAATGAA
    GAACATGAGG TTTTACAGAT GGAGAAAACA GCCAGAACAA AACACTCCCA ACACACTCCA
    ATCTACTGCA ATGACATCTT TAAAGAGGAG AAAGAGCAAA TCAAGACTGT TCTTACTAAA
    GGCATCGCTG GAATCGGAAA AACCGTCTCT GTGCAGAAGT TCATTCTGGA CTGGGCCGAG
    GGAAAAGCCA ATCAGGATGT AGATTTCATG TTTGTGCTTC CATTTCGAGA GCTGAACTTG
    ATCAGAGATC ATCAGTACAG TCTTCACACA CTTCTGCTGG ACTTTCATCC TGAACTTGAA
    GATCTGGAGC CCAAGATTTA TGAGAAGTGT AAAGTTGTGT TCATCTTTGA TGGTCTGGAT
    GAAAGCAGAA TCACACTGAT GTTTTCAGAC GCTCAGAAAG TTTGTGATGT GACTGAGACT
    TCATCAGTGG CTGTGTTGAT GTCAAAGCTG ATGAAAGGAG AGCTGCTTCC CTCTGCTCTC
    ATCTGGATCA CCTCCAGACC AGCAGCAGCC AATCAGATCC CCTCCAAATA CATCAAGCGT
    CTGACAGAAA TTCAGGGATT CACTGAGCCT CAGAAGGAGG AATATTTCAG GAAGAGAATC
    AATGAGAAGC ATCAAGCCAG CAGAATCATC TCACACATCA GAAGAGCAAG AAGCCTCCAC
    ATCATGTGCC ACATACCCGT CTTCTGCTGG ATCTCATCCA CTGTGCTTCA GAAGCTCCTG
    GAAGAAGATC TGAGTGCAGA AATCCCTCAA ACTCTGACTG AAATGTACAT CCACTTCCTG
    CTGATTCAGA TCAACATGAG GAATCAGAAG TATGAAGAGA GAGATCCAGA GAAACTCCTG
    CAGTCTAACA GAGAAGTGAT TGTCAAACTT GCTGAAGTGG CTTTCAGACA GCTGATGAAG
    GGCAATGTGA TGTTCTATGA GGAGGACCTG ATTGAGAGCG GCATCGACAT CTGTGAGGCC
    TCAGTGTGTA CTGGGATTTG CACTGAGATC TTTAAGGAGG AATCTGTGAT TCTTCTGACG
    AAAGTCTACA GCTTCATCCA TCTGAGTGTT CAGGAATTTC TGGCTGCTTT CTATGTGTTT
    TACGTTCATG TAAGCAGCAC TACAGAAGCA TCTTTTGATT CACTAGAAAA TCTCCATAAA
    AGAATCATTG ATAAAGCCAT TGAGAGTGAG AATGGTCATC TGGATCTGTT CCTGCGGTTC
    CTGCTGGGCG TCTCACTGGA GTCCAATCAG AGACTCTTCC AGGATCTACT GACACACACA
    GAGAAGAGCT CAGAGAGCAT CAGGAGAAGC ACACAGTACA TTAAAGAGAA GATCAGAGAT
    GGACATGGAC TCTCCACTGA AAGATCCATC AATCTGTTCC TCTGTCTGCT GGAAGTGAAA
    GATCAGACTC TGTCCAGAGA GATTCAGGAG TTTGTGAAAT CAGACAAACA GTCAGAGAAG
    AAACTCTCTC CTGCTCACTG CTCAACAATA GCCTACATGC TTCAGATGTC AGAGGAGGTG
    CTGGATGAGC TGGAGCTCCA GAAATACAAC ACATCAGATG AGGGTAGAAG AAGACTGATA
    CCAGCCGTCA GCAACTGCAC AAGAGCTCTT CTTGCTGGCT GTAATCTCTC TGCTCAGGAT
    TGTGAAATTG TGTCGTCAGT TCTTCAATCC TCAAACTGTG TCCTGAGAGA GCTGGACCTG
    AGTAACAATG ACTTGCAGGA TTCAGGAGTG AAGCTGCTCT CTGATGGACT GAAGAGTCCA
    AACTGTCAGC TGGAGATACT GAGGTTGTCT GGCTGTATGG TGACAGAGGA AGGCTGTGGT
    TTTCTATCTT CAGCACTGAG TTCAAACCCC TCACACCTGA GAGAGCTGGA TCTGAGCTAC
    AATCACCCAG GACAATCAGG AGTCCAGCTG CTCCAACACA CACTGGAGGA TCCACACTAC
    ACACTGCAGA AACTCAATTT GGACCATGGA GGACATTTGA GGATCAAGCA AGGATTTCTG
    AAATATGCCT GCTTTCTCAC ACTGGATCCA AACACAGCAC ACAATCAACT TCAAGTGTCT
    GATGGAAACA GAAAGGCAGT GCATATGAAA GATCCTCAGC CGTATCCTGA TCATCCAGAG
    AGATTTGAGC TACAGGAGCA GGTTCTGTGT AGAGAGACTC TGACTGGACG CTGTTACTGG
    GAGTCTAAAT GGAGTGGATC TGGTTATGTA GCAGTGGCCT ATAAAGCGAT CGACAGGAAA
    GGAGGGCTTG AATCTTGGTT TGGACTCAAC GAAAGGTCCT GGAGTATTTA CTGCTCCAAT
    ATAAATTACA CCGCCTGGCA CAATAACGAG GGCACTGATA TTATTGCTCC TTCCTCTCGC
    AATAATAGAG TAGGAGTGTA TGTGGACGTG CCGTCCGGTT CTATGTCCTT CTACAGTGTC
    TCTGACACAC ACACACTCAC ACACTTACAC ACATTCAACA CCACATTCAC TGAAGACATC
    TATGCAGGAT TCAGGATATA TCCTAAATCT TCAGTGTCTC TGTGTCAGAT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212339.1
    CDS97..3150
    Translation

    Target ORF information:

    RefSeq Version XM_017356850.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-222h21.10 (si:dkey-222h21.10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356850.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGTGCTC CGTCAAGTCA TGATCATCCT TCCACCTGTG ATGAAAACAT GACCTCTGAC 
    CCCAGGGTAA CTCTGAGAAA AACACCAAGA TTAGAGTCTT CTGGACCCAG TGGTGTGTCT
    GTGAAGAGTG ACTGGTCCAT GGAGCGACCT CCTGCATTGA GCAATCAACC AGTGACCTCT
    GATCCTCGTG TAAATCAGAG AAAGACACCA AGGTTGGAGT CTCCAGAACT CAGTGGTGTG
    TCTGTGAAGA GTGACTGGTC TAAAGAGCGA CCTCCTGCAT TCAGTGATGA ACCAGTGACC
    TCTGACCCTC GGAGGTGTGA TATACATGAG CGATGTGTGA ACATTACACA GAGCAACATT
    GGATCACAAA CACTGAAGAC AAGAGACCTA CAGAGAGTCA AAGACCAGCT CAAAGCCAGC
    ATGAAGAACA AGTATGAGCT GTTATCTGAA GGAATTAAAG TAAAAGAGAA TCAAACCCAC
    CTGAACCGTA TCTACACCCA GCTGTACATC ATAGAAGGAG AGAGTGAAGG AGTGAATGAA
    GAACATGAGG TTTTACAGAT GGAGAAAACA GCCAGAACAA AACACTCCCA ACACACTCCA
    ATCTACTGCA ATGACATCTT TAAAGAGGAG AAAGAGCAAA TCAAGACTGT TCTTACTAAA
    GGCATCGCTG GAATCGGAAA AACCGTCTCT GTGCAGAAGT TCATTCTGGA CTGGGCCGAG
    GGAAAAGCCA ATCAGGATGT AGATTTCATG TTTGTGCTTC CATTTCGAGA GCTGAACTTG
    ATCAGAGATC ATCAGTACAG TCTTCACACA CTTCTGCTGG ACTTTCATCC TGAACTTGAA
    GATCTGGAGC CCAAGATTTA TGAGAAGTGT AAAGTTGTGT TCATCTTTGA TGGTCTGGAT
    GAAAGCAGAA TCACACTGAT GTTTTCAGAC GCTCAGAAAG TTTGTGATGT GACTGAGACT
    TCATCAGTGG CTGTGTTGAT GTCAAAGCTG ATGAAAGGAG AGCTGCTTCC CTCTGCTCTC
    ATCTGGATCA CCTCCAGACC AGCAGCAGCC AATCAGATCC CCTCCAAATA CATCAAGCGT
    CTGACAGAAA TTCAGGGATT CACTGAGCCT CAGAAGGAGG AATATTTCAG GAAGAGAATC
    AATGAGAAGC ATCAAGCCAG CAGAATCATC TCACACATCA GAAGAGCAAG AAGCCTCCAC
    ATCATGTGCC ACATACCCGT CTTCTGCTGG ATCTCATCCA CTGTGCTTCA GAAGCTCCTG
    GAAGAAGATC TGAGTGCAGA AATCCCTCAA ACTCTGACTG AAATGTACAT CCACTTCCTG
    CTGATTCAGA TCAACATGAG GAATCAGAAG TATGAAGAGA GAGATCCAGA GAAACTCCTG
    CAGTCTAACA GAGAAGTGAT TGTCAAACTT GCTGAAGTGG CTTTCAGACA GCTGATGAAG
    GGCAATGTGA TGTTCTATGA GGAGGACCTG ATTGAGAGCG GCATCGACAT CTGTGAGGCC
    TCAGTGTGTA CTGGGATTTG CACTGAGATC TTTAAGGAGG AATCTGTGAT TCTTCTGACG
    AAAGTCTACA GCTTCATCCA TCTGAGTGTT CAGGAATTTC TGGCTGCTTT CTATGTGTTT
    TACGTTCATG TAAGCAGCAC TACAGAAGCA TCTTTTGATT CACTAGAAAA TCTCCATAAA
    AGAATCATTG ATAAAGCCAT TGAGAGTGAG AATGGTCATC TGGATCTGTT CCTGCGGTTC
    CTGCTGGGCG TCTCACTGGA GTCCAATCAG AGACTCTTCC AGGATCTACT GACACACACA
    GAGAAGAGCT CAGAGAGCAT CAGGAGAAGC ACACAGTACA TTAAAGAGAA GATCAGAGAT
    GGACATGGAC TCTCCACTGA AAGATCCATC AATCTGTTCC TCTGTCTGCT GGAAGTGAAA
    GATCAGACTC TGTCCAGAGA GATTCAGGAG TTTGTGAAAT CAGACAAACA GTCAGAGAAG
    AAACTCTCTC CTGCTCACTG CTCAACAATA GCCTACATGC TTCAGATGTC AGAGGAGGTG
    CTGGATGAGC TGGAGCTCCA GAAATACAAC ACATCAGATG AGGGTAGAAG AAGACTGATA
    CCAGCCGTCA GCAACTGCAC AAGAGCTCTT CTTGCTGGCT GTAATCTCTC TGCTCAGGAT
    TGTGAAATTG TGTCGTCAGT TCTTCAATCC TCAAACTGTG TCCTGAGAGA GCTGGACCTG
    AGTAACAATG ACTTGCAGGA TTCAGGAGTG AAGCTGCTCT CTGATGGACT GAAGAGTCCA
    AACTGTCAGC TGGAGATACT GAGGTTGTCT GGCTGTATGG TGACAGAGGA AGGCTGTGGT
    TTTCTATCTT CAGCACTGAG TTCAAACCCC TCACACCTGA GAGAGCTGGA TCTGAGCTAC
    AATCACCCAG GACAATCAGG AGTCCAGCTG CTCCAACACA CACTGGAGGA TCCACACTAC
    ACACTGCAGA AACTCAATTT GGACCATGGA GGACATTTGA GGATCAAGCA AGGATTTCTG
    AAATATGCCT GCTTTCTCAC ACTGGATCCA AACACAGCAC ACAATCAACT TCAAGTGTCT
    GATGGAAACA GAAAGGCAGT GCATATGAAA GATCCTCAGC CGTATCCTGA TCATCCAGAG
    AGATTTGAGC TACAGGAGCA GGTTCTGTGT AGAGAGACTC TGACTGGACG CTGTTACTGG
    GAGTCTAAAT GGAGTGGATC TGGTTATGTA GCAGTGGCCT ATAAAGCGAT CGACAGGAAA
    GGAGGGCTTG AATCTTGGTT TGGACTCAAC GAAAGGTCCT GGAGTATTTA CTGCTCCAAT
    ATAAATTACA CCGCCTGGCA CAATAACGAG GGCACTGATA TTATTGCTCC TTCCTCTCGC
    AATAATAGAG TAGGAGTGTA TGTGGACGTG CCGTCCGGTT CTATGTCCTT CTACAGTGTC
    TCTGACACAC ACACACTCAC ACACTTACAC ACATTCAACA CCACATTCAC TGAAGACATC
    TATGCAGGAT TCAGGATATA TCCTAAATCT TCAGTGTCTC TGTGTCAGAT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53960
    Clone ID Related Accession (Same CDS sequence) XM_017356850.2 , XM_017356850.1
    Accession Version XM_017356850.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3054bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 3-like isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGTGCTC CGTCAAGTCA TGATCATCCT TCCACCTGTG ATGAAAACAT GACCTCTGAC 
    CCCAGGGTAA CTCTGAGAAA AACACCAAGA TTAGAGTCTT CTGGACCCAG TGGTGTGTCT
    GTGAAGAGTG ACTGGTCCAT GGAGCGACCT CCTGCATTGA GCAATCAACC AGTGACCTCT
    GATCCTCGTG TAAATCAGAG AAAGACACCA AGGTTGGAGT CTCCAGAACT CAGTGGTGTG
    TCTGTGAAGA GTGACTGGTC TAAAGAGCGA CCTCCTGCAT TCAGTGATGA ACCAGTGACC
    TCTGACCCTC GGAGGTGTGA TATACATGAG CGATGTGTGA ACATTACACA GAGCAACATT
    GGATCACAAA CACTGAAGAC AAGAGACCTA CAGAGAGTCA AAGACCAGCT CAAAGCCAGC
    ATGAAGAACA AGTATGAGCT GTTATCTGAA GGAATTAAAG TAAAAGAGAA TCAAACCCAC
    CTGAACCGTA TCTACACCCA GCTGTACATC ATAGAAGGAG AGAGTGAAGG AGTGAATGAA
    GAACATGAGG TTTTACAGAT GGAGAAAACA GCCAGAACAA AACACTCCCA ACACACTCCA
    ATCTACTGCA ATGACATCTT TAAAGAGGAG AAAGAGCAAA TCAAGACTGT TCTTACTAAA
    GGCATCGCTG GAATCGGAAA AACCGTCTCT GTGCAGAAGT TCATTCTGGA CTGGGCCGAG
    GGAAAAGCCA ATCAGGATGT AGATTTCATG TTTGTGCTTC CATTTCGAGA GCTGAACTTG
    ATCAGAGATC ATCAGTACAG TCTTCACACA CTTCTGCTGG ACTTTCATCC TGAACTTGAA
    GATCTGGAGC CCAAGATTTA TGAGAAGTGT AAAGTTGTGT TCATCTTTGA TGGTCTGGAT
    GAAAGCAGAA TCACACTGAT GTTTTCAGAC GCTCAGAAAG TTTGTGATGT GACTGAGACT
    TCATCAGTGG CTGTGTTGAT GTCAAAGCTG ATGAAAGGAG AGCTGCTTCC CTCTGCTCTC
    ATCTGGATCA CCTCCAGACC AGCAGCAGCC AATCAGATCC CCTCCAAATA CATCAAGCGT
    CTGACAGAAA TTCAGGGATT CACTGAGCCT CAGAAGGAGG AATATTTCAG GAAGAGAATC
    AATGAGAAGC ATCAAGCCAG CAGAATCATC TCACACATCA GAAGAGCAAG AAGCCTCCAC
    ATCATGTGCC ACATACCCGT CTTCTGCTGG ATCTCATCCA CTGTGCTTCA GAAGCTCCTG
    GAAGAAGATC TGAGTGCAGA AATCCCTCAA ACTCTGACTG AAATGTACAT CCACTTCCTG
    CTGATTCAGA TCAACATGAG GAATCAGAAG TATGAAGAGA GAGATCCAGA GAAACTCCTG
    CAGTCTAACA GAGAAGTGAT TGTCAAACTT GCTGAAGTGG CTTTCAGACA GCTGATGAAG
    GGCAATGTGA TGTTCTATGA GGAGGACCTG ATTGAGAGCG GCATCGACAT CTGTGAGGCC
    TCAGTGTGTA CTGGGATTTG CACTGAGATC TTTAAGGAGG AATCTGTGAT TCTTCTGACG
    AAAGTCTACA GCTTCATCCA TCTGAGTGTT CAGGAATTTC TGGCTGCTTT CTATGTGTTT
    TACGTTCATG TAAGCAGCAC TACAGAAGCA TCTTTTGATT CACTAGAAAA TCTCCATAAA
    AGAATCATTG ATAAAGCCAT TGAGAGTGAG AATGGTCATC TGGATCTGTT CCTGCGGTTC
    CTGCTGGGCG TCTCACTGGA GTCCAATCAG AGACTCTTCC AGGATCTACT GACACACACA
    GAGAAGAGCT CAGAGAGCAT CAGGAGAAGC ACACAGTACA TTAAAGAGAA GATCAGAGAT
    GGACATGGAC TCTCCACTGA AAGATCCATC AATCTGTTCC TCTGTCTGCT GGAAGTGAAA
    GATCAGACTC TGTCCAGAGA GATTCAGGAG TTTGTGAAAT CAGACAAACA GTCAGAGAAG
    AAACTCTCTC CTGCTCACTG CTCAACAATA GCCTACATGC TTCAGATGTC AGAGGAGGTG
    CTGGATGAGC TGGAGCTCCA GAAATACAAC ACATCAGATG AGGGTAGAAG AAGACTGATA
    CCAGCCGTCA GCAACTGCAC AAGAGCTCTT CTTGCTGGCT GTAATCTCTC TGCTCAGGAT
    TGTGAAATTG TGTCGTCAGT TCTTCAATCC TCAAACTGTG TCCTGAGAGA GCTGGACCTG
    AGTAACAATG ACTTGCAGGA TTCAGGAGTG AAGCTGCTCT CTGATGGACT GAAGAGTCCA
    AACTGTCAGC TGGAGATACT GAGGTTGTCT GGCTGTATGG TGACAGAGGA AGGCTGTGGT
    TTTCTATCTT CAGCACTGAG TTCAAACCCC TCACACCTGA GAGAGCTGGA TCTGAGCTAC
    AATCACCCAG GACAATCAGG AGTCCAGCTG CTCCAACACA CACTGGAGGA TCCACACTAC
    ACACTGCAGA AACTCAATTT GGACCATGGA GGACATTTGA GGATCAAGCA AGGATTTCTG
    AAATATGCCT GCTTTCTCAC ACTGGATCCA AACACAGCAC ACAATCAACT TCAAGTGTCT
    GATGGAAACA GAAAGGCAGT GCATATGAAA GATCCTCAGC CGTATCCTGA TCATCCAGAG
    AGATTTGAGC TACAGGAGCA GGTTCTGTGT AGAGAGACTC TGACTGGACG CTGTTACTGG
    GAGTCTAAAT GGAGTGGATC TGGTTATGTA GCAGTGGCCT ATAAAGCGAT CGACAGGAAA
    GGAGGGCTTG AATCTTGGTT TGGACTCAAC GAAAGGTCCT GGAGTATTTA CTGCTCCAAT
    ATAAATTACA CCGCCTGGCA CAATAACGAG GGCACTGATA TTATTGCTCC TTCCTCTCGC
    AATAATAGAG TAGGAGTGTA TGTGGACGTG CCGTCCGGTT CTATGTCCTT CTACAGTGTC
    TCTGACACAC ACACACTCAC ACACTTACAC ACATTCAACA CCACATTCAC TGAAGACATC
    TATGCAGGAT TCAGGATATA TCCTAAATCT TCAGTGTCTC TGTGTCAGAT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212339.1
    CDS97..3150
    Translation

    Target ORF information:

    RefSeq Version XM_017356850.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-222h21.10 (si:dkey-222h21.10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356850.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGTGCTC CGTCAAGTCA TGATCATCCT TCCACCTGTG ATGAAAACAT GACCTCTGAC 
    CCCAGGGTAA CTCTGAGAAA AACACCAAGA TTAGAGTCTT CTGGACCCAG TGGTGTGTCT
    GTGAAGAGTG ACTGGTCCAT GGAGCGACCT CCTGCATTGA GCAATCAACC AGTGACCTCT
    GATCCTCGTG TAAATCAGAG AAAGACACCA AGGTTGGAGT CTCCAGAACT CAGTGGTGTG
    TCTGTGAAGA GTGACTGGTC TAAAGAGCGA CCTCCTGCAT TCAGTGATGA ACCAGTGACC
    TCTGACCCTC GGAGGTGTGA TATACATGAG CGATGTGTGA ACATTACACA GAGCAACATT
    GGATCACAAA CACTGAAGAC AAGAGACCTA CAGAGAGTCA AAGACCAGCT CAAAGCCAGC
    ATGAAGAACA AGTATGAGCT GTTATCTGAA GGAATTAAAG TAAAAGAGAA TCAAACCCAC
    CTGAACCGTA TCTACACCCA GCTGTACATC ATAGAAGGAG AGAGTGAAGG AGTGAATGAA
    GAACATGAGG TTTTACAGAT GGAGAAAACA GCCAGAACAA AACACTCCCA ACACACTCCA
    ATCTACTGCA ATGACATCTT TAAAGAGGAG AAAGAGCAAA TCAAGACTGT TCTTACTAAA
    GGCATCGCTG GAATCGGAAA AACCGTCTCT GTGCAGAAGT TCATTCTGGA CTGGGCCGAG
    GGAAAAGCCA ATCAGGATGT AGATTTCATG TTTGTGCTTC CATTTCGAGA GCTGAACTTG
    ATCAGAGATC ATCAGTACAG TCTTCACACA CTTCTGCTGG ACTTTCATCC TGAACTTGAA
    GATCTGGAGC CCAAGATTTA TGAGAAGTGT AAAGTTGTGT TCATCTTTGA TGGTCTGGAT
    GAAAGCAGAA TCACACTGAT GTTTTCAGAC GCTCAGAAAG TTTGTGATGT GACTGAGACT
    TCATCAGTGG CTGTGTTGAT GTCAAAGCTG ATGAAAGGAG AGCTGCTTCC CTCTGCTCTC
    ATCTGGATCA CCTCCAGACC AGCAGCAGCC AATCAGATCC CCTCCAAATA CATCAAGCGT
    CTGACAGAAA TTCAGGGATT CACTGAGCCT CAGAAGGAGG AATATTTCAG GAAGAGAATC
    AATGAGAAGC ATCAAGCCAG CAGAATCATC TCACACATCA GAAGAGCAAG AAGCCTCCAC
    ATCATGTGCC ACATACCCGT CTTCTGCTGG ATCTCATCCA CTGTGCTTCA GAAGCTCCTG
    GAAGAAGATC TGAGTGCAGA AATCCCTCAA ACTCTGACTG AAATGTACAT CCACTTCCTG
    CTGATTCAGA TCAACATGAG GAATCAGAAG TATGAAGAGA GAGATCCAGA GAAACTCCTG
    CAGTCTAACA GAGAAGTGAT TGTCAAACTT GCTGAAGTGG CTTTCAGACA GCTGATGAAG
    GGCAATGTGA TGTTCTATGA GGAGGACCTG ATTGAGAGCG GCATCGACAT CTGTGAGGCC
    TCAGTGTGTA CTGGGATTTG CACTGAGATC TTTAAGGAGG AATCTGTGAT TCTTCTGACG
    AAAGTCTACA GCTTCATCCA TCTGAGTGTT CAGGAATTTC TGGCTGCTTT CTATGTGTTT
    TACGTTCATG TAAGCAGCAC TACAGAAGCA TCTTTTGATT CACTAGAAAA TCTCCATAAA
    AGAATCATTG ATAAAGCCAT TGAGAGTGAG AATGGTCATC TGGATCTGTT CCTGCGGTTC
    CTGCTGGGCG TCTCACTGGA GTCCAATCAG AGACTCTTCC AGGATCTACT GACACACACA
    GAGAAGAGCT CAGAGAGCAT CAGGAGAAGC ACACAGTACA TTAAAGAGAA GATCAGAGAT
    GGACATGGAC TCTCCACTGA AAGATCCATC AATCTGTTCC TCTGTCTGCT GGAAGTGAAA
    GATCAGACTC TGTCCAGAGA GATTCAGGAG TTTGTGAAAT CAGACAAACA GTCAGAGAAG
    AAACTCTCTC CTGCTCACTG CTCAACAATA GCCTACATGC TTCAGATGTC AGAGGAGGTG
    CTGGATGAGC TGGAGCTCCA GAAATACAAC ACATCAGATG AGGGTAGAAG AAGACTGATA
    CCAGCCGTCA GCAACTGCAC AAGAGCTCTT CTTGCTGGCT GTAATCTCTC TGCTCAGGAT
    TGTGAAATTG TGTCGTCAGT TCTTCAATCC TCAAACTGTG TCCTGAGAGA GCTGGACCTG
    AGTAACAATG ACTTGCAGGA TTCAGGAGTG AAGCTGCTCT CTGATGGACT GAAGAGTCCA
    AACTGTCAGC TGGAGATACT GAGGTTGTCT GGCTGTATGG TGACAGAGGA AGGCTGTGGT
    TTTCTATCTT CAGCACTGAG TTCAAACCCC TCACACCTGA GAGAGCTGGA TCTGAGCTAC
    AATCACCCAG GACAATCAGG AGTCCAGCTG CTCCAACACA CACTGGAGGA TCCACACTAC
    ACACTGCAGA AACTCAATTT GGACCATGGA GGACATTTGA GGATCAAGCA AGGATTTCTG
    AAATATGCCT GCTTTCTCAC ACTGGATCCA AACACAGCAC ACAATCAACT TCAAGTGTCT
    GATGGAAACA GAAAGGCAGT GCATATGAAA GATCCTCAGC CGTATCCTGA TCATCCAGAG
    AGATTTGAGC TACAGGAGCA GGTTCTGTGT AGAGAGACTC TGACTGGACG CTGTTACTGG
    GAGTCTAAAT GGAGTGGATC TGGTTATGTA GCAGTGGCCT ATAAAGCGAT CGACAGGAAA
    GGAGGGCTTG AATCTTGGTT TGGACTCAAC GAAAGGTCCT GGAGTATTTA CTGCTCCAAT
    ATAAATTACA CCGCCTGGCA CAATAACGAG GGCACTGATA TTATTGCTCC TTCCTCTCGC
    AATAATAGAG TAGGAGTGTA TGTGGACGTG CCGTCCGGTT CTATGTCCTT CTACAGTGTC
    TCTGACACAC ACACACTCAC ACACTTACAC ACATTCAACA CCACATTCAC TGAAGACATC
    TATGCAGGAT TCAGGATATA TCCTAAATCT TCAGTGTCTC TGTGTCAGAT TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53959
    Clone ID Related Accession (Same CDS sequence) XM_017356848.2 , XM_017356848.1 , XM_017356849.1
    Accession Version XM_017356848.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 3-like isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356848.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGTGCTC CGTCAAGTCA TGATCATCCT TCCACCTGTG ATGAAAACAT GACCTCTGAC 
    CCCAGGGTAA CTCTGAGAAA AACACCAAGA TTAGAGTCTT CTGGACCCAG TGGTGTGTCT
    GTGAAGAGTG ACTGGTCCAT GGAGCGACCT CCTGCATTGA GCAATCAACC AGTGACCTCT
    GATCCTCGTG TAAATCAGAG AAAGACACCA AGGTTGGAGT CTCCAGAACT CAGTGGTGTG
    TCTGTGAAGA GTGACTGGTC TAAAGAGCGA CCTCCTGCAT TCAGTGATGA ACCAGTGACC
    TCTGACCCTC GTGTAAATCA GAGAAAGACA CCAAGGTTGG AGTCTCCAGA ACTCAGTGGT
    GTGTCTGTGA AGAGTGACTG GTCCATAGAG CGACCTCCTG CATTCAGTAA TGAACCTGTG
    ACCTCTGACC CTCGGAGGTG TGATATACAT GAGCGATGTG TGAACATTAC ACAGAGCAAC
    ATTGGATCAC AAACACTGAA GACAAGAGAC CTACAGAGAG TCAAAGACCA GCTCAAAGCC
    AGCATGAAGA ACAAGTATGA GCTGTTATCT GAAGGAATTA AAGTAAAAGA GAATCAAACC
    CACCTGAACC GTATCTACAC CCAGCTGTAC ATCATAGAAG GAGAGAGTGA AGGAGTGAAT
    GAAGAACATG AGGTTTTACA GATGGAGAAA ACAGCCAGAA CAAAACACTC CCAACACACT
    CCAATCTACT GCAATGACAT CTTTAAAGAG GAGAAAGAGC AAATCAAGAC TGTTCTTACT
    AAAGGCATCG CTGGAATCGG AAAAACCGTC TCTGTGCAGA AGTTCATTCT GGACTGGGCC
    GAGGGAAAAG CCAATCAGGA TGTAGATTTC ATGTTTGTGC TTCCATTTCG AGAGCTGAAC
    TTGATCAGAG ATCATCAGTA CAGTCTTCAC ACACTTCTGC TGGACTTTCA TCCTGAACTT
    GAAGATCTGG AGCCCAAGAT TTATGAGAAG TGTAAAGTTG TGTTCATCTT TGATGGTCTG
    GATGAAAGCA GAATCACACT GATGTTTTCA GACGCTCAGA AAGTTTGTGA TGTGACTGAG
    ACTTCATCAG TGGCTGTGTT GATGTCAAAG CTGATGAAAG GAGAGCTGCT TCCCTCTGCT
    CTCATCTGGA TCACCTCCAG ACCAGCAGCA GCCAATCAGA TCCCCTCCAA ATACATCAAG
    CGTCTGACAG AAATTCAGGG ATTCACTGAG CCTCAGAAGG AGGAATATTT CAGGAAGAGA
    ATCAATGAGA AGCATCAAGC CAGCAGAATC ATCTCACACA TCAGAAGAGC AAGAAGCCTC
    CACATCATGT GCCACATACC CGTCTTCTGC TGGATCTCAT CCACTGTGCT TCAGAAGCTC
    CTGGAAGAAG ATCTGAGTGC AGAAATCCCT CAAACTCTGA CTGAAATGTA CATCCACTTC
    CTGCTGATTC AGATCAACAT GAGGAATCAG AAGTATGAAG AGAGAGATCC AGAGAAACTC
    CTGCAGTCTA ACAGAGAAGT GATTGTCAAA CTTGCTGAAG TGGCTTTCAG ACAGCTGATG
    AAGGGCAATG TGATGTTCTA TGAGGAGGAC CTGATTGAGA GCGGCATCGA CATCTGTGAG
    GCCTCAGTGT GTACTGGGAT TTGCACTGAG ATCTTTAAGG AGGAATCTGT GATTCTTCTG
    ACGAAAGTCT ACAGCTTCAT CCATCTGAGT GTTCAGGAAT TTCTGGCTGC TTTCTATGTG
    TTTTACGTTC ATGTAAGCAG CACTACAGAA GCATCTTTTG ATTCACTAGA AAATCTCCAT
    AAAAGAATCA TTGATAAAGC CATTGAGAGT GAGAATGGTC ATCTGGATCT GTTCCTGCGG
    TTCCTGCTGG GCGTCTCACT GGAGTCCAAT CAGAGACTCT TCCAGGATCT ACTGACACAC
    ACAGAGAAGA GCTCAGAGAG CATCAGGAGA AGCACACAGT ACATTAAAGA GAAGATCAGA
    GATGGACATG GACTCTCCAC TGAAAGATCC ATCAATCTGT TCCTCTGTCT GCTGGAAGTG
    AAAGATCAGA CTCTGTCCAG AGAGATTCAG GAGTTTGTGA AATCAGACAA ACAGTCAGAG
    AAGAAACTCT CTCCTGCTCA CTGCTCAACA ATAGCCTACA TGCTTCAGAT GTCAGAGGAG
    GTGCTGGATG AGCTGGAGCT CCAGAAATAC AACACATCAG ATGAGGGTAG AAGAAGACTG
    ATACCAGCCG TCAGCAACTG CACAAGAGCT CTTCTTGCTG GCTGTAATCT CTCTGCTCAG
    GATTGTGAAA TTGTGTCGTC AGTTCTTCAA TCCTCAAACT GTGTCCTGAG AGAGCTGGAC
    CTGAGTAACA ATGACTTGCA GGATTCAGGA GTGAAGCTGC TCTCTGATGG ACTGAAGAGT
    CCAAACTGTC AGCTGGAGAT ACTGAGGTTG TCTGGCTGTA TGGTGACAGA GGAAGGCTGT
    GGTTTTCTAT CTTCAGCACT GAGTTCAAAC CCCTCACACC TGAGAGAGCT GGATCTGAGC
    TACAATCACC CAGGACAATC AGGAGTCCAG CTGCTCCAAC ACACACTGGA GGATCCACAC
    TACACACTGC AGAAACTCAA TTTGGACCAT GGAGGACATT TGAGGATCAA GCAAGGATTT
    CTGAAATATG CCTGCTTTCT CACACTGGAT CCAAACACAG CACACAATCA ACTTCAAGTG
    TCTGATGGAA ACAGAAAGGC AGTGCATATG AAAGATCCTC AGCCGTATCC TGATCATCCA
    GAGAGATTTG AGCTACAGGA GCAGGTTCTG TGTAGAGAGA CTCTGACTGG ACGCTGTTAC
    TGGGAGTCTA AATGGAGTGG ATCTGGTTAT GTAGCAGTGG CCTATAAAGC GATCGACAGG
    AAAGGAGGGC TTGAATCTTG GTTTGGACTC AACGAAAGGT CCTGGAGTAT TTACTGCTCC
    AATATAAATT ACACCGCCTG GCACAATAAC GAGGGCACTG ATATTATTGC TCCTTCCTCT
    CGCAATAATA GAGTAGGAGT GTATGTGGAC GTGCCGTCCG GTTCTATGTC CTTCTACAGT
    GTCTCTGACA CACACACACT CACACACTTA CACACATTCA ACACCACATT CACTGAAGAC
    ATCTATGCAG GATTCAGGAT ATATCCTAAA TCTTCAGTGT CTCTGTGTCA GATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212337.1
    CDS97..3273
    Translation

    Target ORF information:

    RefSeq Version XM_017356848.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-222h21.10 (si:dkey-222h21.10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356848.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGTGCTC CGTCAAGTCA TGATCATCCT TCCACCTGTG ATGAAAACAT GACCTCTGAC 
    CCCAGGGTAA CTCTGAGAAA AACACCAAGA TTAGAGTCTT CTGGACCCAG TGGTGTGTCT
    GTGAAGAGTG ACTGGTCCAT GGAGCGACCT CCTGCATTGA GCAATCAACC AGTGACCTCT
    GATCCTCGTG TAAATCAGAG AAAGACACCA AGGTTGGAGT CTCCAGAACT CAGTGGTGTG
    TCTGTGAAGA GTGACTGGTC TAAAGAGCGA CCTCCTGCAT TCAGTGATGA ACCAGTGACC
    TCTGACCCTC GTGTAAATCA GAGAAAGACA CCAAGGTTGG AGTCTCCAGA ACTCAGTGGT
    GTGTCTGTGA AGAGTGACTG GTCCATAGAG CGACCTCCTG CATTCAGTAA TGAACCTGTG
    ACCTCTGACC CTCGGAGGTG TGATATACAT GAGCGATGTG TGAACATTAC ACAGAGCAAC
    ATTGGATCAC AAACACTGAA GACAAGAGAC CTACAGAGAG TCAAAGACCA GCTCAAAGCC
    AGCATGAAGA ACAAGTATGA GCTGTTATCT GAAGGAATTA AAGTAAAAGA GAATCAAACC
    CACCTGAACC GTATCTACAC CCAGCTGTAC ATCATAGAAG GAGAGAGTGA AGGAGTGAAT
    GAAGAACATG AGGTTTTACA GATGGAGAAA ACAGCCAGAA CAAAACACTC CCAACACACT
    CCAATCTACT GCAATGACAT CTTTAAAGAG GAGAAAGAGC AAATCAAGAC TGTTCTTACT
    AAAGGCATCG CTGGAATCGG AAAAACCGTC TCTGTGCAGA AGTTCATTCT GGACTGGGCC
    GAGGGAAAAG CCAATCAGGA TGTAGATTTC ATGTTTGTGC TTCCATTTCG AGAGCTGAAC
    TTGATCAGAG ATCATCAGTA CAGTCTTCAC ACACTTCTGC TGGACTTTCA TCCTGAACTT
    GAAGATCTGG AGCCCAAGAT TTATGAGAAG TGTAAAGTTG TGTTCATCTT TGATGGTCTG
    GATGAAAGCA GAATCACACT GATGTTTTCA GACGCTCAGA AAGTTTGTGA TGTGACTGAG
    ACTTCATCAG TGGCTGTGTT GATGTCAAAG CTGATGAAAG GAGAGCTGCT TCCCTCTGCT
    CTCATCTGGA TCACCTCCAG ACCAGCAGCA GCCAATCAGA TCCCCTCCAA ATACATCAAG
    CGTCTGACAG AAATTCAGGG ATTCACTGAG CCTCAGAAGG AGGAATATTT CAGGAAGAGA
    ATCAATGAGA AGCATCAAGC CAGCAGAATC ATCTCACACA TCAGAAGAGC AAGAAGCCTC
    CACATCATGT GCCACATACC CGTCTTCTGC TGGATCTCAT CCACTGTGCT TCAGAAGCTC
    CTGGAAGAAG ATCTGAGTGC AGAAATCCCT CAAACTCTGA CTGAAATGTA CATCCACTTC
    CTGCTGATTC AGATCAACAT GAGGAATCAG AAGTATGAAG AGAGAGATCC AGAGAAACTC
    CTGCAGTCTA ACAGAGAAGT GATTGTCAAA CTTGCTGAAG TGGCTTTCAG ACAGCTGATG
    AAGGGCAATG TGATGTTCTA TGAGGAGGAC CTGATTGAGA GCGGCATCGA CATCTGTGAG
    GCCTCAGTGT GTACTGGGAT TTGCACTGAG ATCTTTAAGG AGGAATCTGT GATTCTTCTG
    ACGAAAGTCT ACAGCTTCAT CCATCTGAGT GTTCAGGAAT TTCTGGCTGC TTTCTATGTG
    TTTTACGTTC ATGTAAGCAG CACTACAGAA GCATCTTTTG ATTCACTAGA AAATCTCCAT
    AAAAGAATCA TTGATAAAGC CATTGAGAGT GAGAATGGTC ATCTGGATCT GTTCCTGCGG
    TTCCTGCTGG GCGTCTCACT GGAGTCCAAT CAGAGACTCT TCCAGGATCT ACTGACACAC
    ACAGAGAAGA GCTCAGAGAG CATCAGGAGA AGCACACAGT ACATTAAAGA GAAGATCAGA
    GATGGACATG GACTCTCCAC TGAAAGATCC ATCAATCTGT TCCTCTGTCT GCTGGAAGTG
    AAAGATCAGA CTCTGTCCAG AGAGATTCAG GAGTTTGTGA AATCAGACAA ACAGTCAGAG
    AAGAAACTCT CTCCTGCTCA CTGCTCAACA ATAGCCTACA TGCTTCAGAT GTCAGAGGAG
    GTGCTGGATG AGCTGGAGCT CCAGAAATAC AACACATCAG ATGAGGGTAG AAGAAGACTG
    ATACCAGCCG TCAGCAACTG CACAAGAGCT CTTCTTGCTG GCTGTAATCT CTCTGCTCAG
    GATTGTGAAA TTGTGTCGTC AGTTCTTCAA TCCTCAAACT GTGTCCTGAG AGAGCTGGAC
    CTGAGTAACA ATGACTTGCA GGATTCAGGA GTGAAGCTGC TCTCTGATGG ACTGAAGAGT
    CCAAACTGTC AGCTGGAGAT ACTGAGGTTG TCTGGCTGTA TGGTGACAGA GGAAGGCTGT
    GGTTTTCTAT CTTCAGCACT GAGTTCAAAC CCCTCACACC TGAGAGAGCT GGATCTGAGC
    TACAATCACC CAGGACAATC AGGAGTCCAG CTGCTCCAAC ACACACTGGA GGATCCACAC
    TACACACTGC AGAAACTCAA TTTGGACCAT GGAGGACATT TGAGGATCAA GCAAGGATTT
    CTGAAATATG CCTGCTTTCT CACACTGGAT CCAAACACAG CACACAATCA ACTTCAAGTG
    TCTGATGGAA ACAGAAAGGC AGTGCATATG AAAGATCCTC AGCCGTATCC TGATCATCCA
    GAGAGATTTG AGCTACAGGA GCAGGTTCTG TGTAGAGAGA CTCTGACTGG ACGCTGTTAC
    TGGGAGTCTA AATGGAGTGG ATCTGGTTAT GTAGCAGTGG CCTATAAAGC GATCGACAGG
    AAAGGAGGGC TTGAATCTTG GTTTGGACTC AACGAAAGGT CCTGGAGTAT TTACTGCTCC
    AATATAAATT ACACCGCCTG GCACAATAAC GAGGGCACTG ATATTATTGC TCCTTCCTCT
    CGCAATAATA GAGTAGGAGT GTATGTGGAC GTGCCGTCCG GTTCTATGTC CTTCTACAGT
    GTCTCTGACA CACACACACT CACACACTTA CACACATTCA ACACCACATT CACTGAAGAC
    ATCTATGCAG GATTCAGGAT ATATCCTAAA TCTTCAGTGT CTCTGTGTCA GATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53959
    Clone ID Related Accession (Same CDS sequence) XM_017356848.2 , XM_017356848.1 , XM_017356849.1
    Accession Version XM_017356848.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 3-like isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGTGCTC CGTCAAGTCA TGATCATCCT TCCACCTGTG ATGAAAACAT GACCTCTGAC 
    CCCAGGGTAA CTCTGAGAAA AACACCAAGA TTAGAGTCTT CTGGACCCAG TGGTGTGTCT
    GTGAAGAGTG ACTGGTCCAT GGAGCGACCT CCTGCATTGA GCAATCAACC AGTGACCTCT
    GATCCTCGTG TAAATCAGAG AAAGACACCA AGGTTGGAGT CTCCAGAACT CAGTGGTGTG
    TCTGTGAAGA GTGACTGGTC TAAAGAGCGA CCTCCTGCAT TCAGTGATGA ACCAGTGACC
    TCTGACCCTC GTGTAAATCA GAGAAAGACA CCAAGGTTGG AGTCTCCAGA ACTCAGTGGT
    GTGTCTGTGA AGAGTGACTG GTCCATAGAG CGACCTCCTG CATTCAGTAA TGAACCTGTG
    ACCTCTGACC CTCGGAGGTG TGATATACAT GAGCGATGTG TGAACATTAC ACAGAGCAAC
    ATTGGATCAC AAACACTGAA GACAAGAGAC CTACAGAGAG TCAAAGACCA GCTCAAAGCC
    AGCATGAAGA ACAAGTATGA GCTGTTATCT GAAGGAATTA AAGTAAAAGA GAATCAAACC
    CACCTGAACC GTATCTACAC CCAGCTGTAC ATCATAGAAG GAGAGAGTGA AGGAGTGAAT
    GAAGAACATG AGGTTTTACA GATGGAGAAA ACAGCCAGAA CAAAACACTC CCAACACACT
    CCAATCTACT GCAATGACAT CTTTAAAGAG GAGAAAGAGC AAATCAAGAC TGTTCTTACT
    AAAGGCATCG CTGGAATCGG AAAAACCGTC TCTGTGCAGA AGTTCATTCT GGACTGGGCC
    GAGGGAAAAG CCAATCAGGA TGTAGATTTC ATGTTTGTGC TTCCATTTCG AGAGCTGAAC
    TTGATCAGAG ATCATCAGTA CAGTCTTCAC ACACTTCTGC TGGACTTTCA TCCTGAACTT
    GAAGATCTGG AGCCCAAGAT TTATGAGAAG TGTAAAGTTG TGTTCATCTT TGATGGTCTG
    GATGAAAGCA GAATCACACT GATGTTTTCA GACGCTCAGA AAGTTTGTGA TGTGACTGAG
    ACTTCATCAG TGGCTGTGTT GATGTCAAAG CTGATGAAAG GAGAGCTGCT TCCCTCTGCT
    CTCATCTGGA TCACCTCCAG ACCAGCAGCA GCCAATCAGA TCCCCTCCAA ATACATCAAG
    CGTCTGACAG AAATTCAGGG ATTCACTGAG CCTCAGAAGG AGGAATATTT CAGGAAGAGA
    ATCAATGAGA AGCATCAAGC CAGCAGAATC ATCTCACACA TCAGAAGAGC AAGAAGCCTC
    CACATCATGT GCCACATACC CGTCTTCTGC TGGATCTCAT CCACTGTGCT TCAGAAGCTC
    CTGGAAGAAG ATCTGAGTGC AGAAATCCCT CAAACTCTGA CTGAAATGTA CATCCACTTC
    CTGCTGATTC AGATCAACAT GAGGAATCAG AAGTATGAAG AGAGAGATCC AGAGAAACTC
    CTGCAGTCTA ACAGAGAAGT GATTGTCAAA CTTGCTGAAG TGGCTTTCAG ACAGCTGATG
    AAGGGCAATG TGATGTTCTA TGAGGAGGAC CTGATTGAGA GCGGCATCGA CATCTGTGAG
    GCCTCAGTGT GTACTGGGAT TTGCACTGAG ATCTTTAAGG AGGAATCTGT GATTCTTCTG
    ACGAAAGTCT ACAGCTTCAT CCATCTGAGT GTTCAGGAAT TTCTGGCTGC TTTCTATGTG
    TTTTACGTTC ATGTAAGCAG CACTACAGAA GCATCTTTTG ATTCACTAGA AAATCTCCAT
    AAAAGAATCA TTGATAAAGC CATTGAGAGT GAGAATGGTC ATCTGGATCT GTTCCTGCGG
    TTCCTGCTGG GCGTCTCACT GGAGTCCAAT CAGAGACTCT TCCAGGATCT ACTGACACAC
    ACAGAGAAGA GCTCAGAGAG CATCAGGAGA AGCACACAGT ACATTAAAGA GAAGATCAGA
    GATGGACATG GACTCTCCAC TGAAAGATCC ATCAATCTGT TCCTCTGTCT GCTGGAAGTG
    AAAGATCAGA CTCTGTCCAG AGAGATTCAG GAGTTTGTGA AATCAGACAA ACAGTCAGAG
    AAGAAACTCT CTCCTGCTCA CTGCTCAACA ATAGCCTACA TGCTTCAGAT GTCAGAGGAG
    GTGCTGGATG AGCTGGAGCT CCAGAAATAC AACACATCAG ATGAGGGTAG AAGAAGACTG
    ATACCAGCCG TCAGCAACTG CACAAGAGCT CTTCTTGCTG GCTGTAATCT CTCTGCTCAG
    GATTGTGAAA TTGTGTCGTC AGTTCTTCAA TCCTCAAACT GTGTCCTGAG AGAGCTGGAC
    CTGAGTAACA ATGACTTGCA GGATTCAGGA GTGAAGCTGC TCTCTGATGG ACTGAAGAGT
    CCAAACTGTC AGCTGGAGAT ACTGAGGTTG TCTGGCTGTA TGGTGACAGA GGAAGGCTGT
    GGTTTTCTAT CTTCAGCACT GAGTTCAAAC CCCTCACACC TGAGAGAGCT GGATCTGAGC
    TACAATCACC CAGGACAATC AGGAGTCCAG CTGCTCCAAC ACACACTGGA GGATCCACAC
    TACACACTGC AGAAACTCAA TTTGGACCAT GGAGGACATT TGAGGATCAA GCAAGGATTT
    CTGAAATATG CCTGCTTTCT CACACTGGAT CCAAACACAG CACACAATCA ACTTCAAGTG
    TCTGATGGAA ACAGAAAGGC AGTGCATATG AAAGATCCTC AGCCGTATCC TGATCATCCA
    GAGAGATTTG AGCTACAGGA GCAGGTTCTG TGTAGAGAGA CTCTGACTGG ACGCTGTTAC
    TGGGAGTCTA AATGGAGTGG ATCTGGTTAT GTAGCAGTGG CCTATAAAGC GATCGACAGG
    AAAGGAGGGC TTGAATCTTG GTTTGGACTC AACGAAAGGT CCTGGAGTAT TTACTGCTCC
    AATATAAATT ACACCGCCTG GCACAATAAC GAGGGCACTG ATATTATTGC TCCTTCCTCT
    CGCAATAATA GAGTAGGAGT GTATGTGGAC GTGCCGTCCG GTTCTATGTC CTTCTACAGT
    GTCTCTGACA CACACACACT CACACACTTA CACACATTCA ACACCACATT CACTGAAGAC
    ATCTATGCAG GATTCAGGAT ATATCCTAAA TCTTCAGTGT CTCTGTGTCA GATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212337.1
    CDS97..3273
    Translation

    Target ORF information:

    RefSeq Version XM_017356848.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-222h21.10 (si:dkey-222h21.10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356848.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGTGCTC CGTCAAGTCA TGATCATCCT TCCACCTGTG ATGAAAACAT GACCTCTGAC 
    CCCAGGGTAA CTCTGAGAAA AACACCAAGA TTAGAGTCTT CTGGACCCAG TGGTGTGTCT
    GTGAAGAGTG ACTGGTCCAT GGAGCGACCT CCTGCATTGA GCAATCAACC AGTGACCTCT
    GATCCTCGTG TAAATCAGAG AAAGACACCA AGGTTGGAGT CTCCAGAACT CAGTGGTGTG
    TCTGTGAAGA GTGACTGGTC TAAAGAGCGA CCTCCTGCAT TCAGTGATGA ACCAGTGACC
    TCTGACCCTC GTGTAAATCA GAGAAAGACA CCAAGGTTGG AGTCTCCAGA ACTCAGTGGT
    GTGTCTGTGA AGAGTGACTG GTCCATAGAG CGACCTCCTG CATTCAGTAA TGAACCTGTG
    ACCTCTGACC CTCGGAGGTG TGATATACAT GAGCGATGTG TGAACATTAC ACAGAGCAAC
    ATTGGATCAC AAACACTGAA GACAAGAGAC CTACAGAGAG TCAAAGACCA GCTCAAAGCC
    AGCATGAAGA ACAAGTATGA GCTGTTATCT GAAGGAATTA AAGTAAAAGA GAATCAAACC
    CACCTGAACC GTATCTACAC CCAGCTGTAC ATCATAGAAG GAGAGAGTGA AGGAGTGAAT
    GAAGAACATG AGGTTTTACA GATGGAGAAA ACAGCCAGAA CAAAACACTC CCAACACACT
    CCAATCTACT GCAATGACAT CTTTAAAGAG GAGAAAGAGC AAATCAAGAC TGTTCTTACT
    AAAGGCATCG CTGGAATCGG AAAAACCGTC TCTGTGCAGA AGTTCATTCT GGACTGGGCC
    GAGGGAAAAG CCAATCAGGA TGTAGATTTC ATGTTTGTGC TTCCATTTCG AGAGCTGAAC
    TTGATCAGAG ATCATCAGTA CAGTCTTCAC ACACTTCTGC TGGACTTTCA TCCTGAACTT
    GAAGATCTGG AGCCCAAGAT TTATGAGAAG TGTAAAGTTG TGTTCATCTT TGATGGTCTG
    GATGAAAGCA GAATCACACT GATGTTTTCA GACGCTCAGA AAGTTTGTGA TGTGACTGAG
    ACTTCATCAG TGGCTGTGTT GATGTCAAAG CTGATGAAAG GAGAGCTGCT TCCCTCTGCT
    CTCATCTGGA TCACCTCCAG ACCAGCAGCA GCCAATCAGA TCCCCTCCAA ATACATCAAG
    CGTCTGACAG AAATTCAGGG ATTCACTGAG CCTCAGAAGG AGGAATATTT CAGGAAGAGA
    ATCAATGAGA AGCATCAAGC CAGCAGAATC ATCTCACACA TCAGAAGAGC AAGAAGCCTC
    CACATCATGT GCCACATACC CGTCTTCTGC TGGATCTCAT CCACTGTGCT TCAGAAGCTC
    CTGGAAGAAG ATCTGAGTGC AGAAATCCCT CAAACTCTGA CTGAAATGTA CATCCACTTC
    CTGCTGATTC AGATCAACAT GAGGAATCAG AAGTATGAAG AGAGAGATCC AGAGAAACTC
    CTGCAGTCTA ACAGAGAAGT GATTGTCAAA CTTGCTGAAG TGGCTTTCAG ACAGCTGATG
    AAGGGCAATG TGATGTTCTA TGAGGAGGAC CTGATTGAGA GCGGCATCGA CATCTGTGAG
    GCCTCAGTGT GTACTGGGAT TTGCACTGAG ATCTTTAAGG AGGAATCTGT GATTCTTCTG
    ACGAAAGTCT ACAGCTTCAT CCATCTGAGT GTTCAGGAAT TTCTGGCTGC TTTCTATGTG
    TTTTACGTTC ATGTAAGCAG CACTACAGAA GCATCTTTTG ATTCACTAGA AAATCTCCAT
    AAAAGAATCA TTGATAAAGC CATTGAGAGT GAGAATGGTC ATCTGGATCT GTTCCTGCGG
    TTCCTGCTGG GCGTCTCACT GGAGTCCAAT CAGAGACTCT TCCAGGATCT ACTGACACAC
    ACAGAGAAGA GCTCAGAGAG CATCAGGAGA AGCACACAGT ACATTAAAGA GAAGATCAGA
    GATGGACATG GACTCTCCAC TGAAAGATCC ATCAATCTGT TCCTCTGTCT GCTGGAAGTG
    AAAGATCAGA CTCTGTCCAG AGAGATTCAG GAGTTTGTGA AATCAGACAA ACAGTCAGAG
    AAGAAACTCT CTCCTGCTCA CTGCTCAACA ATAGCCTACA TGCTTCAGAT GTCAGAGGAG
    GTGCTGGATG AGCTGGAGCT CCAGAAATAC AACACATCAG ATGAGGGTAG AAGAAGACTG
    ATACCAGCCG TCAGCAACTG CACAAGAGCT CTTCTTGCTG GCTGTAATCT CTCTGCTCAG
    GATTGTGAAA TTGTGTCGTC AGTTCTTCAA TCCTCAAACT GTGTCCTGAG AGAGCTGGAC
    CTGAGTAACA ATGACTTGCA GGATTCAGGA GTGAAGCTGC TCTCTGATGG ACTGAAGAGT
    CCAAACTGTC AGCTGGAGAT ACTGAGGTTG TCTGGCTGTA TGGTGACAGA GGAAGGCTGT
    GGTTTTCTAT CTTCAGCACT GAGTTCAAAC CCCTCACACC TGAGAGAGCT GGATCTGAGC
    TACAATCACC CAGGACAATC AGGAGTCCAG CTGCTCCAAC ACACACTGGA GGATCCACAC
    TACACACTGC AGAAACTCAA TTTGGACCAT GGAGGACATT TGAGGATCAA GCAAGGATTT
    CTGAAATATG CCTGCTTTCT CACACTGGAT CCAAACACAG CACACAATCA ACTTCAAGTG
    TCTGATGGAA ACAGAAAGGC AGTGCATATG AAAGATCCTC AGCCGTATCC TGATCATCCA
    GAGAGATTTG AGCTACAGGA GCAGGTTCTG TGTAGAGAGA CTCTGACTGG ACGCTGTTAC
    TGGGAGTCTA AATGGAGTGG ATCTGGTTAT GTAGCAGTGG CCTATAAAGC GATCGACAGG
    AAAGGAGGGC TTGAATCTTG GTTTGGACTC AACGAAAGGT CCTGGAGTAT TTACTGCTCC
    AATATAAATT ACACCGCCTG GCACAATAAC GAGGGCACTG ATATTATTGC TCCTTCCTCT
    CGCAATAATA GAGTAGGAGT GTATGTGGAC GTGCCGTCCG GTTCTATGTC CTTCTACAGT
    GTCTCTGACA CACACACACT CACACACTTA CACACATTCA ACACCACATT CACTGAAGAC
    ATCTATGCAG GATTCAGGAT ATATCCTAAA TCTTCAGTGT CTCTGTGTCA GATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53959
    Clone ID Related Accession (Same CDS sequence) XM_017356848.2 , XM_017356848.1 , XM_017356849.1
    Accession Version XM_017356849.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 3-like isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGTGCTC CGTCAAGTCA TGATCATCCT TCCACCTGTG ATGAAAACAT GACCTCTGAC 
    CCCAGGGTAA CTCTGAGAAA AACACCAAGA TTAGAGTCTT CTGGACCCAG TGGTGTGTCT
    GTGAAGAGTG ACTGGTCCAT GGAGCGACCT CCTGCATTGA GCAATCAACC AGTGACCTCT
    GATCCTCGTG TAAATCAGAG AAAGACACCA AGGTTGGAGT CTCCAGAACT CAGTGGTGTG
    TCTGTGAAGA GTGACTGGTC TAAAGAGCGA CCTCCTGCAT TCAGTGATGA ACCAGTGACC
    TCTGACCCTC GTGTAAATCA GAGAAAGACA CCAAGGTTGG AGTCTCCAGA ACTCAGTGGT
    GTGTCTGTGA AGAGTGACTG GTCCATAGAG CGACCTCCTG CATTCAGTAA TGAACCTGTG
    ACCTCTGACC CTCGGAGGTG TGATATACAT GAGCGATGTG TGAACATTAC ACAGAGCAAC
    ATTGGATCAC AAACACTGAA GACAAGAGAC CTACAGAGAG TCAAAGACCA GCTCAAAGCC
    AGCATGAAGA ACAAGTATGA GCTGTTATCT GAAGGAATTA AAGTAAAAGA GAATCAAACC
    CACCTGAACC GTATCTACAC CCAGCTGTAC ATCATAGAAG GAGAGAGTGA AGGAGTGAAT
    GAAGAACATG AGGTTTTACA GATGGAGAAA ACAGCCAGAA CAAAACACTC CCAACACACT
    CCAATCTACT GCAATGACAT CTTTAAAGAG GAGAAAGAGC AAATCAAGAC TGTTCTTACT
    AAAGGCATCG CTGGAATCGG AAAAACCGTC TCTGTGCAGA AGTTCATTCT GGACTGGGCC
    GAGGGAAAAG CCAATCAGGA TGTAGATTTC ATGTTTGTGC TTCCATTTCG AGAGCTGAAC
    TTGATCAGAG ATCATCAGTA CAGTCTTCAC ACACTTCTGC TGGACTTTCA TCCTGAACTT
    GAAGATCTGG AGCCCAAGAT TTATGAGAAG TGTAAAGTTG TGTTCATCTT TGATGGTCTG
    GATGAAAGCA GAATCACACT GATGTTTTCA GACGCTCAGA AAGTTTGTGA TGTGACTGAG
    ACTTCATCAG TGGCTGTGTT GATGTCAAAG CTGATGAAAG GAGAGCTGCT TCCCTCTGCT
    CTCATCTGGA TCACCTCCAG ACCAGCAGCA GCCAATCAGA TCCCCTCCAA ATACATCAAG
    CGTCTGACAG AAATTCAGGG ATTCACTGAG CCTCAGAAGG AGGAATATTT CAGGAAGAGA
    ATCAATGAGA AGCATCAAGC CAGCAGAATC ATCTCACACA TCAGAAGAGC AAGAAGCCTC
    CACATCATGT GCCACATACC CGTCTTCTGC TGGATCTCAT CCACTGTGCT TCAGAAGCTC
    CTGGAAGAAG ATCTGAGTGC AGAAATCCCT CAAACTCTGA CTGAAATGTA CATCCACTTC
    CTGCTGATTC AGATCAACAT GAGGAATCAG AAGTATGAAG AGAGAGATCC AGAGAAACTC
    CTGCAGTCTA ACAGAGAAGT GATTGTCAAA CTTGCTGAAG TGGCTTTCAG ACAGCTGATG
    AAGGGCAATG TGATGTTCTA TGAGGAGGAC CTGATTGAGA GCGGCATCGA CATCTGTGAG
    GCCTCAGTGT GTACTGGGAT TTGCACTGAG ATCTTTAAGG AGGAATCTGT GATTCTTCTG
    ACGAAAGTCT ACAGCTTCAT CCATCTGAGT GTTCAGGAAT TTCTGGCTGC TTTCTATGTG
    TTTTACGTTC ATGTAAGCAG CACTACAGAA GCATCTTTTG ATTCACTAGA AAATCTCCAT
    AAAAGAATCA TTGATAAAGC CATTGAGAGT GAGAATGGTC ATCTGGATCT GTTCCTGCGG
    TTCCTGCTGG GCGTCTCACT GGAGTCCAAT CAGAGACTCT TCCAGGATCT ACTGACACAC
    ACAGAGAAGA GCTCAGAGAG CATCAGGAGA AGCACACAGT ACATTAAAGA GAAGATCAGA
    GATGGACATG GACTCTCCAC TGAAAGATCC ATCAATCTGT TCCTCTGTCT GCTGGAAGTG
    AAAGATCAGA CTCTGTCCAG AGAGATTCAG GAGTTTGTGA AATCAGACAA ACAGTCAGAG
    AAGAAACTCT CTCCTGCTCA CTGCTCAACA ATAGCCTACA TGCTTCAGAT GTCAGAGGAG
    GTGCTGGATG AGCTGGAGCT CCAGAAATAC AACACATCAG ATGAGGGTAG AAGAAGACTG
    ATACCAGCCG TCAGCAACTG CACAAGAGCT CTTCTTGCTG GCTGTAATCT CTCTGCTCAG
    GATTGTGAAA TTGTGTCGTC AGTTCTTCAA TCCTCAAACT GTGTCCTGAG AGAGCTGGAC
    CTGAGTAACA ATGACTTGCA GGATTCAGGA GTGAAGCTGC TCTCTGATGG ACTGAAGAGT
    CCAAACTGTC AGCTGGAGAT ACTGAGGTTG TCTGGCTGTA TGGTGACAGA GGAAGGCTGT
    GGTTTTCTAT CTTCAGCACT GAGTTCAAAC CCCTCACACC TGAGAGAGCT GGATCTGAGC
    TACAATCACC CAGGACAATC AGGAGTCCAG CTGCTCCAAC ACACACTGGA GGATCCACAC
    TACACACTGC AGAAACTCAA TTTGGACCAT GGAGGACATT TGAGGATCAA GCAAGGATTT
    CTGAAATATG CCTGCTTTCT CACACTGGAT CCAAACACAG CACACAATCA ACTTCAAGTG
    TCTGATGGAA ACAGAAAGGC AGTGCATATG AAAGATCCTC AGCCGTATCC TGATCATCCA
    GAGAGATTTG AGCTACAGGA GCAGGTTCTG TGTAGAGAGA CTCTGACTGG ACGCTGTTAC
    TGGGAGTCTA AATGGAGTGG ATCTGGTTAT GTAGCAGTGG CCTATAAAGC GATCGACAGG
    AAAGGAGGGC TTGAATCTTG GTTTGGACTC AACGAAAGGT CCTGGAGTAT TTACTGCTCC
    AATATAAATT ACACCGCCTG GCACAATAAC GAGGGCACTG ATATTATTGC TCCTTCCTCT
    CGCAATAATA GAGTAGGAGT GTATGTGGAC GTGCCGTCCG GTTCTATGTC CTTCTACAGT
    GTCTCTGACA CACACACACT CACACACTTA CACACATTCA ACACCACATT CACTGAAGAC
    ATCTATGCAG GATTCAGGAT ATATCCTAAA TCTTCAGTGT CTCTGTGTCA GATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017212338.1
    CDS244..3420
    Translation

    Target ORF information:

    RefSeq Version XM_017356849.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:dkey-222h21.10 (si:dkey-222h21.10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356849.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGAGTGCTC CGTCAAGTCA TGATCATCCT TCCACCTGTG ATGAAAACAT GACCTCTGAC 
    CCCAGGGTAA CTCTGAGAAA AACACCAAGA TTAGAGTCTT CTGGACCCAG TGGTGTGTCT
    GTGAAGAGTG ACTGGTCCAT GGAGCGACCT CCTGCATTGA GCAATCAACC AGTGACCTCT
    GATCCTCGTG TAAATCAGAG AAAGACACCA AGGTTGGAGT CTCCAGAACT CAGTGGTGTG
    TCTGTGAAGA GTGACTGGTC TAAAGAGCGA CCTCCTGCAT TCAGTGATGA ACCAGTGACC
    TCTGACCCTC GTGTAAATCA GAGAAAGACA CCAAGGTTGG AGTCTCCAGA ACTCAGTGGT
    GTGTCTGTGA AGAGTGACTG GTCCATAGAG CGACCTCCTG CATTCAGTAA TGAACCTGTG
    ACCTCTGACC CTCGGAGGTG TGATATACAT GAGCGATGTG TGAACATTAC ACAGAGCAAC
    ATTGGATCAC AAACACTGAA GACAAGAGAC CTACAGAGAG TCAAAGACCA GCTCAAAGCC
    AGCATGAAGA ACAAGTATGA GCTGTTATCT GAAGGAATTA AAGTAAAAGA GAATCAAACC
    CACCTGAACC GTATCTACAC CCAGCTGTAC ATCATAGAAG GAGAGAGTGA AGGAGTGAAT
    GAAGAACATG AGGTTTTACA GATGGAGAAA ACAGCCAGAA CAAAACACTC CCAACACACT
    CCAATCTACT GCAATGACAT CTTTAAAGAG GAGAAAGAGC AAATCAAGAC TGTTCTTACT
    AAAGGCATCG CTGGAATCGG AAAAACCGTC TCTGTGCAGA AGTTCATTCT GGACTGGGCC
    GAGGGAAAAG CCAATCAGGA TGTAGATTTC ATGTTTGTGC TTCCATTTCG AGAGCTGAAC
    TTGATCAGAG ATCATCAGTA CAGTCTTCAC ACACTTCTGC TGGACTTTCA TCCTGAACTT
    GAAGATCTGG AGCCCAAGAT TTATGAGAAG TGTAAAGTTG TGTTCATCTT TGATGGTCTG
    GATGAAAGCA GAATCACACT GATGTTTTCA GACGCTCAGA AAGTTTGTGA TGTGACTGAG
    ACTTCATCAG TGGCTGTGTT GATGTCAAAG CTGATGAAAG GAGAGCTGCT TCCCTCTGCT
    CTCATCTGGA TCACCTCCAG ACCAGCAGCA GCCAATCAGA TCCCCTCCAA ATACATCAAG
    CGTCTGACAG AAATTCAGGG ATTCACTGAG CCTCAGAAGG AGGAATATTT CAGGAAGAGA
    ATCAATGAGA AGCATCAAGC CAGCAGAATC ATCTCACACA TCAGAAGAGC AAGAAGCCTC
    CACATCATGT GCCACATACC CGTCTTCTGC TGGATCTCAT CCACTGTGCT TCAGAAGCTC
    CTGGAAGAAG ATCTGAGTGC AGAAATCCCT CAAACTCTGA CTGAAATGTA CATCCACTTC
    CTGCTGATTC AGATCAACAT GAGGAATCAG AAGTATGAAG AGAGAGATCC AGAGAAACTC
    CTGCAGTCTA ACAGAGAAGT GATTGTCAAA CTTGCTGAAG TGGCTTTCAG ACAGCTGATG
    AAGGGCAATG TGATGTTCTA TGAGGAGGAC CTGATTGAGA GCGGCATCGA CATCTGTGAG
    GCCTCAGTGT GTACTGGGAT TTGCACTGAG ATCTTTAAGG AGGAATCTGT GATTCTTCTG
    ACGAAAGTCT ACAGCTTCAT CCATCTGAGT GTTCAGGAAT TTCTGGCTGC TTTCTATGTG
    TTTTACGTTC ATGTAAGCAG CACTACAGAA GCATCTTTTG ATTCACTAGA AAATCTCCAT
    AAAAGAATCA TTGATAAAGC CATTGAGAGT GAGAATGGTC ATCTGGATCT GTTCCTGCGG
    TTCCTGCTGG GCGTCTCACT GGAGTCCAAT CAGAGACTCT TCCAGGATCT ACTGACACAC
    ACAGAGAAGA GCTCAGAGAG CATCAGGAGA AGCACACAGT ACATTAAAGA GAAGATCAGA
    GATGGACATG GACTCTCCAC TGAAAGATCC ATCAATCTGT TCCTCTGTCT GCTGGAAGTG
    AAAGATCAGA CTCTGTCCAG AGAGATTCAG GAGTTTGTGA AATCAGACAA ACAGTCAGAG
    AAGAAACTCT CTCCTGCTCA CTGCTCAACA ATAGCCTACA TGCTTCAGAT GTCAGAGGAG
    GTGCTGGATG AGCTGGAGCT CCAGAAATAC AACACATCAG ATGAGGGTAG AAGAAGACTG
    ATACCAGCCG TCAGCAACTG CACAAGAGCT CTTCTTGCTG GCTGTAATCT CTCTGCTCAG
    GATTGTGAAA TTGTGTCGTC AGTTCTTCAA TCCTCAAACT GTGTCCTGAG AGAGCTGGAC
    CTGAGTAACA ATGACTTGCA GGATTCAGGA GTGAAGCTGC TCTCTGATGG ACTGAAGAGT
    CCAAACTGTC AGCTGGAGAT ACTGAGGTTG TCTGGCTGTA TGGTGACAGA GGAAGGCTGT
    GGTTTTCTAT CTTCAGCACT GAGTTCAAAC CCCTCACACC TGAGAGAGCT GGATCTGAGC
    TACAATCACC CAGGACAATC AGGAGTCCAG CTGCTCCAAC ACACACTGGA GGATCCACAC
    TACACACTGC AGAAACTCAA TTTGGACCAT GGAGGACATT TGAGGATCAA GCAAGGATTT
    CTGAAATATG CCTGCTTTCT CACACTGGAT CCAAACACAG CACACAATCA ACTTCAAGTG
    TCTGATGGAA ACAGAAAGGC AGTGCATATG AAAGATCCTC AGCCGTATCC TGATCATCCA
    GAGAGATTTG AGCTACAGGA GCAGGTTCTG TGTAGAGAGA CTCTGACTGG ACGCTGTTAC
    TGGGAGTCTA AATGGAGTGG ATCTGGTTAT GTAGCAGTGG CCTATAAAGC GATCGACAGG
    AAAGGAGGGC TTGAATCTTG GTTTGGACTC AACGAAAGGT CCTGGAGTAT TTACTGCTCC
    AATATAAATT ACACCGCCTG GCACAATAAC GAGGGCACTG ATATTATTGC TCCTTCCTCT
    CGCAATAATA GAGTAGGAGT GTATGTGGAC GTGCCGTCCG GTTCTATGTC CTTCTACAGT
    GTCTCTGACA CACACACACT CACACACTTA CACACATTCA ACACCACATT CACTGAAGAC
    ATCTATGCAG GATTCAGGAT ATATCCTAAA TCTTCAGTGT CTCTGTGTCA GATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.