si:dkey-253d23.10 cDNA ORF clone, Danio rerio(zebrafish)

The following si:dkey-253d23.10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:dkey-253d23.10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa151325 XM_021469560.1
Latest version!
Danio rerio si:dkey-253d23.10 (si:dkey-253d23.10), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa151326 XM_021469561.1
Latest version!
Danio rerio si:dkey-253d23.10 (si:dkey-253d23.10), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa151327 XM_021469562.1
Latest version!
Danio rerio si:dkey-253d23.10 (si:dkey-253d23.10), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa151328 XM_021469563.1
Latest version!
Danio rerio si:dkey-253d23.10 (si:dkey-253d23.10), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa151325
    Clone ID Related Accession (Same CDS sequence) XM_021469560.1
    Accession Version XM_021469560.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4143bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 12 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCAGAGG AGCAGGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAT ATCAGACTCA 
    TGTGTGTCCG GCTCTGTGTC TGTGAAGAGC GACTGCTCAA AAGATGGTGA TCTCCCGAAC
    TTCAGTGTGG AAAAGCCAAT GTCTGCTAAA AGTGTAATAT CAGACTCATG TGTGTCCGGC
    TCTGTGTCTG TGAAGAGCGA CTGCTCAAAA GATGGTGATC TCCCGAACTT CAGTGTGGAA
    AAACAAACGT CTGCTAAAAG TGTAATATCA GACTCCTGTG TGTCCGGCTC TGTGTTTGTG
    AAGAGTGACT GCTCAAAAGA TGGTGATCTC CCGAACTTCA GCGAGGAAAC ACCGTCATCT
    GGCAAAAGTT TTGAACATAA GACATCAGAC TCAGACTTCC AGACTCACAG GAAACACAAG
    GGGTTTCAAG ACGGTCTTCT GGGGATCTTT CAGGATCTTG AAAACCAGAT AATGAATTAT
    CTGAAGCATG AGCTAGAAAA GTTTAAGAAA ATCTTACAAA AAGAAAACAT AATTGACTTT
    GTAAAGGACT TTAACGAGAA CAGATGCAGC ATCAAAGAAG CAGCTCTCGA TCTCACACTC
    TACTTCCTGA GGGAAATGAA GCAAGAGGAA AGTGCTGATA CTCTAGAAGA CGAACTGATC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGATTCAAG ACTTCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGCTTCTAGA
    CGTCATGAAG CACAAGAGCT ACAGGTTGAA TGCAGACACT TGTTTGAAGC GTCTGAACAA
    GATAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC GGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTTGTGATGT TTCGTCGCCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACCGG CTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAAGAGG AGTACTTCAG AAAAAGACTG ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA AAACATTTTA CAGGAGAAGA GAAATAATGA TGTTAAACAC
    AATCAAGCTG ATGACGCCTC CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CGCTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATAATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTCATCATCT GTTAAACAAC AACCTAATCT TCTATGACAC AGACCTGGAA
    GCCTGTGGTA TTGACGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CTGGGATCAT TCTTGGTACC ATGTACTGCT TTGTTCACCT GACTCTTCAA
    GAGTTTATCG CAGCCCTTTA TGCACATCTG ATTCTAGACA CTAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAAAAT AAATGAGTCC ATGATTGATT TGCTGAAGAC TGCAGTGGAC
    AAGGCACTAG AGAGTGACAA TGGGCACCTG GACCTTTTTC TTCGCTTCCT TCTCGGTCTG
    TCACTTCAGT CCAATCGACG AATCTTACGA GGTCTGTTGA CACCGCAAGA CCAAGATGAC
    CAAAGCACAG AGAAAATACT TCAGTATATC AAGCAGAAGT TTGATGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGAACAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG CTGACTTTTC ATCTGCCCAC
    TGGTCTGCTT TGGCCTTTGT GTTGTTGACA TCAGATGAGG AGATGGAGGA GTTTGAGCTT
    CAGAAATTCC ATAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTCAT CAAAACCTGC
    AAAAGAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TTTGGCTACA
    GTTCTTGAGT CAGATACCAG TCTAAAAGAG CTGAACATGA GTAATAATAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG TACTGGACTG AAGGATATGA AGTGTGAATT GGAGATACTG
    AGACTTTCAG ACTGCAATAT TACAGAGGAA GGTTATAAAG CACTGGCTTC AGCTCTGAGA
    TCAAATCCTT CACATCTACT AGAGCTAGAC CTCAGAGGAA ATGATCCTGG AGAATCAGGA
    GTAAAGGAGC TTGATGATTT ACTGCAGGAT CCAAACTGTA CACTGAAAAC ACTGAGGTTT
    TTAAGTCCTT CTGCAGAAGA ATCCTGTCAG TTTGTGAATG GGGTTGTGGG TAAAAACCCA
    TTACTCCTAA AAACACTGAA TCTGAATGAT TTCCGATCCA CGCGTAACCA TCCATTTATA
    TATGAAATAG AAGACACACA AGTGAATCAC CTCGCTGCTC TACTGCAGGA TAAACACTGT
    AGAGTCAACA TGCTTATTCT GGGTGGATGC AGTTTTACAG ATGATCAGTG GGAGAGCCTA
    ACTTCAGCTC TGAAATTAAA CCCATCACAC CTGAGAGAAC TGATCCTGAT GTGGAATGGA
    CCGGGAGAGT CTGGAGTGAA AATCCTCAGT GATTTCCTAA TCGAGCCACA ATTAAAGCTG
    GAGAAACTAT GTTTGTGTTA TTGCAGTATT ACAGAGGAAC GGTGTGAGAT CCTGACTTCA
    GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGAACC TCAGTCAGAA TATACCTGGC
    GACTCTGGAA TGAAAAACCT CAGTGATTTA CTGATGAATC CACAATCCAA GCTGGAGAAA
    CTACAACTTT GTAAATGCAG TATTACAGAG GAACAGTGTG TGATCCTGAC TTCAGCTCTG
    AAATCAAACC CATCACACCT GAGAGATCTG AACCTCAGTC TGAATAATCT AGGAGACTCT
    GGAGTGAAAA CTCTAAGTGA TTTACTGATG AAGCCACACT TAAAGATGGA AAAACTATGG
    CTGTGTAAAT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGGCTTCAGC TCTGAAATCA
    AACCCATCAC ACCTGAGAGA ACTGAGCCTC AGTGAGAATA ATCTGGGAGA CTCTGGAGTG
    AAAAACCTCA GTGATTTACT GATGAAGCCA CAGTTCAAGC TGGAGAAACT ACATCTGTCT
    CATTGCAGTA TCACAGAGGA ACAGTGCGAG ATCCTGACTT CAGCTCTGAA ATCAAACCCA
    TCACACCTGA GAGAACTGGA CATAAGTAAG AATCAAATTA GAAACACTGG AGTGAATCTC
    TTATGTGACG TACTGAACAA TTCAGACTGC AAACTGGAGA CACTGAGACT AAACGACTGT
    GGCATCACAG ATGTTTCTTC TTTAACTCAG TGTTTGACGA ACACAAAAGC TCTGCAGTTT
    CTAAAAGTGC TTTATCTGAG GGAAAATCAC ATTGGAGACT CAAAGCAGGA GCTCAGTGAT
    GTGGTACGAG ACTCAAACTG TGAACTGAGG TCAGTAAACA ACACAGACTG TGTTGAAAAT
    TATTATGTTT TATTTAAACA AAGGGTTTGT CTGAGCACAA AGTGTGCAGT GTGTCTGCGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325235.1
    CDS456..4598
    Translation

    Target ORF information:

    RefSeq Version XM_021469560.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253d23.10 (si:dkey-253d23.10), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469560.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGTCAGAGG AGCAGGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAT ATCAGACTCA 
    TGTGTGTCCG GCTCTGTGTC TGTGAAGAGC GACTGCTCAA AAGATGGTGA TCTCCCGAAC
    TTCAGTGTGG AAAAGCCAAT GTCTGCTAAA AGTGTAATAT CAGACTCATG TGTGTCCGGC
    TCTGTGTCTG TGAAGAGCGA CTGCTCAAAA GATGGTGATC TCCCGAACTT CAGTGTGGAA
    AAACAAACGT CTGCTAAAAG TGTAATATCA GACTCCTGTG TGTCCGGCTC TGTGTTTGTG
    AAGAGTGACT GCTCAAAAGA TGGTGATCTC CCGAACTTCA GCGAGGAAAC ACCGTCATCT
    GGCAAAAGTT TTGAACATAA GACATCAGAC TCAGACTTCC AGACTCACAG GAAACACAAG
    GGGTTTCAAG ACGGTCTTCT GGGGATCTTT CAGGATCTTG AAAACCAGAT AATGAATTAT
    CTGAAGCATG AGCTAGAAAA GTTTAAGAAA ATCTTACAAA AAGAAAACAT AATTGACTTT
    GTAAAGGACT TTAACGAGAA CAGATGCAGC ATCAAAGAAG CAGCTCTCGA TCTCACACTC
    TACTTCCTGA GGGAAATGAA GCAAGAGGAA AGTGCTGATA CTCTAGAAGA CGAACTGATC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGATTCAAG ACTTCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGCTTCTAGA
    CGTCATGAAG CACAAGAGCT ACAGGTTGAA TGCAGACACT TGTTTGAAGC GTCTGAACAA
    GATAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC GGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTTGTGATGT TTCGTCGCCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACCGG CTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAAGAGG AGTACTTCAG AAAAAGACTG ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA AAACATTTTA CAGGAGAAGA GAAATAATGA TGTTAAACAC
    AATCAAGCTG ATGACGCCTC CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CGCTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATAATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTCATCATCT GTTAAACAAC AACCTAATCT TCTATGACAC AGACCTGGAA
    GCCTGTGGTA TTGACGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CTGGGATCAT TCTTGGTACC ATGTACTGCT TTGTTCACCT GACTCTTCAA
    GAGTTTATCG CAGCCCTTTA TGCACATCTG ATTCTAGACA CTAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAAAAT AAATGAGTCC ATGATTGATT TGCTGAAGAC TGCAGTGGAC
    AAGGCACTAG AGAGTGACAA TGGGCACCTG GACCTTTTTC TTCGCTTCCT TCTCGGTCTG
    TCACTTCAGT CCAATCGACG AATCTTACGA GGTCTGTTGA CACCGCAAGA CCAAGATGAC
    CAAAGCACAG AGAAAATACT TCAGTATATC AAGCAGAAGT TTGATGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGAACAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG CTGACTTTTC ATCTGCCCAC
    TGGTCTGCTT TGGCCTTTGT GTTGTTGACA TCAGATGAGG AGATGGAGGA GTTTGAGCTT
    CAGAAATTCC ATAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTCAT CAAAACCTGC
    AAAAGAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TTTGGCTACA
    GTTCTTGAGT CAGATACCAG TCTAAAAGAG CTGAACATGA GTAATAATAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG TACTGGACTG AAGGATATGA AGTGTGAATT GGAGATACTG
    AGACTTTCAG ACTGCAATAT TACAGAGGAA GGTTATAAAG CACTGGCTTC AGCTCTGAGA
    TCAAATCCTT CACATCTACT AGAGCTAGAC CTCAGAGGAA ATGATCCTGG AGAATCAGGA
    GTAAAGGAGC TTGATGATTT ACTGCAGGAT CCAAACTGTA CACTGAAAAC ACTGAGGTTT
    TTAAGTCCTT CTGCAGAAGA ATCCTGTCAG TTTGTGAATG GGGTTGTGGG TAAAAACCCA
    TTACTCCTAA AAACACTGAA TCTGAATGAT TTCCGATCCA CGCGTAACCA TCCATTTATA
    TATGAAATAG AAGACACACA AGTGAATCAC CTCGCTGCTC TACTGCAGGA TAAACACTGT
    AGAGTCAACA TGCTTATTCT GGGTGGATGC AGTTTTACAG ATGATCAGTG GGAGAGCCTA
    ACTTCAGCTC TGAAATTAAA CCCATCACAC CTGAGAGAAC TGATCCTGAT GTGGAATGGA
    CCGGGAGAGT CTGGAGTGAA AATCCTCAGT GATTTCCTAA TCGAGCCACA ATTAAAGCTG
    GAGAAACTAT GTTTGTGTTA TTGCAGTATT ACAGAGGAAC GGTGTGAGAT CCTGACTTCA
    GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGAACC TCAGTCAGAA TATACCTGGC
    GACTCTGGAA TGAAAAACCT CAGTGATTTA CTGATGAATC CACAATCCAA GCTGGAGAAA
    CTACAACTTT GTAAATGCAG TATTACAGAG GAACAGTGTG TGATCCTGAC TTCAGCTCTG
    AAATCAAACC CATCACACCT GAGAGATCTG AACCTCAGTC TGAATAATCT AGGAGACTCT
    GGAGTGAAAA CTCTAAGTGA TTTACTGATG AAGCCACACT TAAAGATGGA AAAACTATGG
    CTGTGTAAAT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGGCTTCAGC TCTGAAATCA
    AACCCATCAC ACCTGAGAGA ACTGAGCCTC AGTGAGAATA ATCTGGGAGA CTCTGGAGTG
    AAAAACCTCA GTGATTTACT GATGAAGCCA CAGTTCAAGC TGGAGAAACT ACATCTGTCT
    CATTGCAGTA TCACAGAGGA ACAGTGCGAG ATCCTGACTT CAGCTCTGAA ATCAAACCCA
    TCACACCTGA GAGAACTGGA CATAAGTAAG AATCAAATTA GAAACACTGG AGTGAATCTC
    TTATGTGACG TACTGAACAA TTCAGACTGC AAACTGGAGA CACTGAGACT AAACGACTGT
    GGCATCACAG ATGTTTCTTC TTTAACTCAG TGTTTGACGA ACACAAAAGC TCTGCAGTTT
    CTAAAAGTGC TTTATCTGAG GGAAAATCAC ATTGGAGACT CAAAGCAGGA GCTCAGTGAT
    GTGGTACGAG ACTCAAACTG TGAACTGAGG TCAGTAAACA ACACAGACTG TGTTGAAAAT
    TATTATGTTT TATTTAAACA AAGGGTTTGT CTGAGCACAA AGTGTGCAGT GTGTCTGCGG
    TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151326
    Clone ID Related Accession (Same CDS sequence) XM_021469561.1
    Accession Version XM_021469561.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4113bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 12 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCAGAGG AGCAGGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAT ATCAGACTCA 
    TGTGTGTCCG GCTCTGTGTC TGTGAAGAGC GACTGCTCAA AAGATGGTGA TCTCCCGAAC
    TTCAGTGTGG AAAAGCCAAT GTCTGCTAAA AGTGTAATAT CAGACTCATG TGTGTCCGGC
    TCTGTGTCTG TGAAGAGCGA CTGCTCAAAA GATGGTGATC TCCCGAACTT CAGTGTGGAA
    AAACAAACGT CTGCTAAAAG TGTAATATCA GACTCCTGTG TGTCCGGCTC TGTGTTTGTG
    AAGAGTGACT GCTCAAAAGA TGGTGATCTC CCGAACTTCA GCGAGGAAAC ACCGTCATCT
    GGCAAAAGTT TTGAACATAA GACATCAGAC TCAGACTTCC AGACTCACAG GAAACACAAG
    GGGTTTCAAG ACGGTCTTCT GGGGATCTTT CAGGATCTTG AAAACCAGAT AATGAATTAT
    CTGAAGCATG AGCTAGAAAA GTTTAAGAAA ATCTTACAAA AAGAAAACAT AATTGACTTT
    GTAAAGGACT TTAACGAGAA CAGATGCAGC ATCAAAGAAG CAGCTCTCGA TCTCACACTC
    TACTTCCTGA GGGAAATGAA GCAAGAGGAA AGTGCTGATA CTCTAGAAGA CGAACTGATC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGATTCAAG ACTTCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGCTTCTAGA
    CGTCATGAAG CACAAGAGCT ACAGGTTGAA TGCAGACACT TGTTTGAAGC GTCTGAACAA
    GATAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC GGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTTGTGATGT TTCGTCGCCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACCGG CTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAAGAGG AGTACTTCAG AAAAAGACTG ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA AAACATTTTA CAGGAGAAGA GAAATAATGA TGTTAAACAC
    AATCAAGCTG ATGACGCCTC CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CGCTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATAATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTCATCATCT GTTAAACAAC AACCTAATCT TCTATGACAC AGACCTGGAA
    GCCTGTGGTA TTGACGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CTGGGATCAT TCTTGGTACC ATGTACTGCT TTGTTCACCT GACTCTTCAA
    GAGTTTATCG CAGCCCTTTA TGCACATCTG ATTCTAGACA CTAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAAAAT AAATGAGTCC ATGATTGATT TGCTGAAGAC TGCAGTGGAC
    AAGGCACTAG AGAGTGACAA TGGGCACCTG GACCTTTTTC TTCGCTTCCT TCTCGGTCTG
    TCACTTCAGT CCAATCGACG AATCTTACGA GGTCTGTTGA CACCGCAAGA CCAAGATGAC
    CAAAGCACAG AGAAAATACT TCAGTATATC AAGCAGAAGT TTGATGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGAACAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG CTGACTTTTC ATCTGCCCAC
    TGGTCTGCTT TGGCCTTTGT GTTGTTGACA TCAGATGAGG AGATGGAGGA GTTTGAGCTT
    CAGAAATTCC ATAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTCAT CAAAACCTGC
    AAAAGAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TTTGGCTACA
    GTTCTTGAGT CAGATACCAG TCTAAAAGAG CTGAACATGA GTAATAATAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG TACTGGACTG AAGGATATGA AGTGTGAATT GGAGATACTG
    AGACTTTCAG ACTGCAATAT TACAGAGGAA GGTTATAAAG CACTGGCTTC AGCTCTGAGA
    TCAAATCCTT CACATCTACT AGAGCTAGAC CTCAGAGGAA ATGATCCTGG AGAATCAGGA
    GTAAAGGAGC TTGATGATTT ACTGCAGGAT CCAAACTGTA CACTGAAAAC ACTGAGGTTT
    TTAAGTCCTT CTGCAGAAGA ATCCTGTCAG TTTGTGAATG GGGTTGTGGG TAAAAACCCA
    TTACTCCTAA AAACACTGAA TCTGAATGAT TTCCGATCCA CGCGTAACCA TCCATTTATA
    TATGAAATAG AAGACACACA AGTGAATCAC CTCGCTGCTC TACTGCAGGA TAAACACTGT
    AGAGTCAACA TGCTTATTCT GGGTGGATGC AGTTTTACAG ATGATCAGTG GGAGAGCCTA
    ACTTCAGCTC TGAAATTAAA CCCATCACAC CTGAGAGAAC TGATCCTGAT GTGGAATGGA
    CCGGGAGAGT CTGGAGTGAA AATCCTCAGT GATTTCCTAA TCGAGCCACA ATTAAAGCTG
    GAGAAACTAT GTTTGTGTTA TTGCAGTATT ACAGAGGAAC GGTGTGAGAT CCTGACTTCA
    GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGAACC TCAGTCAGAA TATACCTGGC
    GACTCTGGAA TGAAAAACCT CAGTGATTTA CTGATGAATC CACAATCCAA GCTGGAGAAA
    CTACAACTTT GTAAATGCAG TATTACAGAG GAACAGTGTG TGATCCTGAC TTCAGCTCTG
    AAATCAAACC CATCACACCT GAGAGATCTG AACCTCAGTC TGAATAATCT AGGAGACTCT
    GGAGTGAAAA CTCTAAGTGA TTTACTGATG AAGCCACACT TAAAGATGGA AAAACTATGG
    CTGTGTAAAT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGGCTTCAGC TCTGAAATCA
    AACCCATCAC ACCTGAGAGA ACTGAGCCTC AGTGAGAATA ATCTGGGAGA CTCTGGAGTG
    AAAAACCTCA GTGATTTACT GATGAAGCCA CAGTTCAAGC TGGAGAAACT ACATCTGTCT
    CATTGCAGTA TCACAGAGGA ACAGTGCGAG ATCCTGACTT CAGCTCTGAA ATCAAACCCA
    TCACACCTGA GAGAACTGGA CATAAGTAAG AATCAAATTA GAAACACTGG AGTGAATCTC
    TTATGTGACG TACTGAACAA TTCAGACTGC AAACTGGAGA CACTGAGACT AAACGACTGT
    GGCATCACAG ATGTTTCTTC TTTAACTCAG TGTTTGACGA ACACAAAAGC TCTGCAGTTT
    CTAAAAGTGC TTTATCTGAG GGAAAATCAC ATTGGAGACT CAAAGCAGGA GCTCAGTGAT
    GTGGTACGAG ACTCAAACTG TGAACTGAGT GTTGATGAAT TTTCACTGCC AGACTTTGGT
    CAAATCAAAG ACATTAAATG CTGTATATCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325236.1
    CDS456..4568
    Translation

    Target ORF information:

    RefSeq Version XM_021469561.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253d23.10 (si:dkey-253d23.10), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469561.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGTCAGAGG AGCAGGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAT ATCAGACTCA 
    TGTGTGTCCG GCTCTGTGTC TGTGAAGAGC GACTGCTCAA AAGATGGTGA TCTCCCGAAC
    TTCAGTGTGG AAAAGCCAAT GTCTGCTAAA AGTGTAATAT CAGACTCATG TGTGTCCGGC
    TCTGTGTCTG TGAAGAGCGA CTGCTCAAAA GATGGTGATC TCCCGAACTT CAGTGTGGAA
    AAACAAACGT CTGCTAAAAG TGTAATATCA GACTCCTGTG TGTCCGGCTC TGTGTTTGTG
    AAGAGTGACT GCTCAAAAGA TGGTGATCTC CCGAACTTCA GCGAGGAAAC ACCGTCATCT
    GGCAAAAGTT TTGAACATAA GACATCAGAC TCAGACTTCC AGACTCACAG GAAACACAAG
    GGGTTTCAAG ACGGTCTTCT GGGGATCTTT CAGGATCTTG AAAACCAGAT AATGAATTAT
    CTGAAGCATG AGCTAGAAAA GTTTAAGAAA ATCTTACAAA AAGAAAACAT AATTGACTTT
    GTAAAGGACT TTAACGAGAA CAGATGCAGC ATCAAAGAAG CAGCTCTCGA TCTCACACTC
    TACTTCCTGA GGGAAATGAA GCAAGAGGAA AGTGCTGATA CTCTAGAAGA CGAACTGATC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGATTCAAG ACTTCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGCTTCTAGA
    CGTCATGAAG CACAAGAGCT ACAGGTTGAA TGCAGACACT TGTTTGAAGC GTCTGAACAA
    GATAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC GGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTTGTGATGT TTCGTCGCCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACCGG CTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAAGAGG AGTACTTCAG AAAAAGACTG ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA AAACATTTTA CAGGAGAAGA GAAATAATGA TGTTAAACAC
    AATCAAGCTG ATGACGCCTC CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CGCTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATAATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTCATCATCT GTTAAACAAC AACCTAATCT TCTATGACAC AGACCTGGAA
    GCCTGTGGTA TTGACGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CTGGGATCAT TCTTGGTACC ATGTACTGCT TTGTTCACCT GACTCTTCAA
    GAGTTTATCG CAGCCCTTTA TGCACATCTG ATTCTAGACA CTAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAAAAT AAATGAGTCC ATGATTGATT TGCTGAAGAC TGCAGTGGAC
    AAGGCACTAG AGAGTGACAA TGGGCACCTG GACCTTTTTC TTCGCTTCCT TCTCGGTCTG
    TCACTTCAGT CCAATCGACG AATCTTACGA GGTCTGTTGA CACCGCAAGA CCAAGATGAC
    CAAAGCACAG AGAAAATACT TCAGTATATC AAGCAGAAGT TTGATGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGAACAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG CTGACTTTTC ATCTGCCCAC
    TGGTCTGCTT TGGCCTTTGT GTTGTTGACA TCAGATGAGG AGATGGAGGA GTTTGAGCTT
    CAGAAATTCC ATAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTCAT CAAAACCTGC
    AAAAGAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TTTGGCTACA
    GTTCTTGAGT CAGATACCAG TCTAAAAGAG CTGAACATGA GTAATAATAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG TACTGGACTG AAGGATATGA AGTGTGAATT GGAGATACTG
    AGACTTTCAG ACTGCAATAT TACAGAGGAA GGTTATAAAG CACTGGCTTC AGCTCTGAGA
    TCAAATCCTT CACATCTACT AGAGCTAGAC CTCAGAGGAA ATGATCCTGG AGAATCAGGA
    GTAAAGGAGC TTGATGATTT ACTGCAGGAT CCAAACTGTA CACTGAAAAC ACTGAGGTTT
    TTAAGTCCTT CTGCAGAAGA ATCCTGTCAG TTTGTGAATG GGGTTGTGGG TAAAAACCCA
    TTACTCCTAA AAACACTGAA TCTGAATGAT TTCCGATCCA CGCGTAACCA TCCATTTATA
    TATGAAATAG AAGACACACA AGTGAATCAC CTCGCTGCTC TACTGCAGGA TAAACACTGT
    AGAGTCAACA TGCTTATTCT GGGTGGATGC AGTTTTACAG ATGATCAGTG GGAGAGCCTA
    ACTTCAGCTC TGAAATTAAA CCCATCACAC CTGAGAGAAC TGATCCTGAT GTGGAATGGA
    CCGGGAGAGT CTGGAGTGAA AATCCTCAGT GATTTCCTAA TCGAGCCACA ATTAAAGCTG
    GAGAAACTAT GTTTGTGTTA TTGCAGTATT ACAGAGGAAC GGTGTGAGAT CCTGACTTCA
    GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGAACC TCAGTCAGAA TATACCTGGC
    GACTCTGGAA TGAAAAACCT CAGTGATTTA CTGATGAATC CACAATCCAA GCTGGAGAAA
    CTACAACTTT GTAAATGCAG TATTACAGAG GAACAGTGTG TGATCCTGAC TTCAGCTCTG
    AAATCAAACC CATCACACCT GAGAGATCTG AACCTCAGTC TGAATAATCT AGGAGACTCT
    GGAGTGAAAA CTCTAAGTGA TTTACTGATG AAGCCACACT TAAAGATGGA AAAACTATGG
    CTGTGTAAAT GCAGTATTAC AGAGGAACAG TGTGTGATCC TGGCTTCAGC TCTGAAATCA
    AACCCATCAC ACCTGAGAGA ACTGAGCCTC AGTGAGAATA ATCTGGGAGA CTCTGGAGTG
    AAAAACCTCA GTGATTTACT GATGAAGCCA CAGTTCAAGC TGGAGAAACT ACATCTGTCT
    CATTGCAGTA TCACAGAGGA ACAGTGCGAG ATCCTGACTT CAGCTCTGAA ATCAAACCCA
    TCACACCTGA GAGAACTGGA CATAAGTAAG AATCAAATTA GAAACACTGG AGTGAATCTC
    TTATGTGACG TACTGAACAA TTCAGACTGC AAACTGGAGA CACTGAGACT AAACGACTGT
    GGCATCACAG ATGTTTCTTC TTTAACTCAG TGTTTGACGA ACACAAAAGC TCTGCAGTTT
    CTAAAAGTGC TTTATCTGAG GGAAAATCAC ATTGGAGACT CAAAGCAGGA GCTCAGTGAT
    GTGGTACGAG ACTCAAACTG TGAACTGAGT GTTGATGAAT TTTCACTGCC AGACTTTGGT
    CAAATCAAAG ACATTAAATG CTGTATATCA TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151327
    Clone ID Related Accession (Same CDS sequence) XM_021469562.1
    Accession Version XM_021469562.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3795bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NLR family CARD domain-containing protein 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCAGAGG AGCAGGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAT ATCAGACTCA 
    TGTGTGTCCG GCTCTGTGTC TGTGAAGAGC GACTGCTCAA AAGATGGTGA TCTCCCGAAC
    TTCAGTGTGG AAAAGCCAAT GTCTGCTAAA AGTGTAATAT CAGACTCATG TGTGTCCGGC
    TCTGTGTCTG TGAAGAGCGA CTGCTCAAAA GATGGTGATC TCCCGAACTT CAGTGTGGAA
    AAACAAACGT CTGCTAAAAG TGTAATATCA GACTCCTGTG TGTCCGGCTC TGTGTTTGTG
    AAGAGTGACT GCTCAAAAGA TGGTGATCTC CCGAACTTCA GCGAGGAAAC ACCGTCATCT
    GGCAAAAGTT TTGAACATAA GACATCAGAC TCAGACTTCC AGACTCACAG GAAACACAAG
    GGGTTTCAAG ACGGTCTTCT GGGGATCTTT CAGGATCTTG AAAACCAGAT AATGAATTAT
    CTGAAGCATG AGCTAGAAAA GTTTAAGAAA ATCTTACAAA AAGAAAACAT AATTGACTTT
    GTAAAGGACT TTAACGAGAA CAGATGCAGC ATCAAAGAAG CAGCTCTCGA TCTCACACTC
    TACTTCCTGA GGGAAATGAA GCAAGAGGAA AGTGCTGATA CTCTAGAAGA CGAACTGATC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGATTCAAG ACTTCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGCTTCTAGA
    CGTCATGAAG CACAAGAGCT ACAGGTTGAA TGCAGACACT TGTTTGAAGC GTCTGAACAA
    GATAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC GGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTTGTGATGT TTCGTCGCCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACCGG CTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAAGAGG AGTACTTCAG AAAAAGACTG ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA AAACATTTTA CAGGAGAAGA GAAATAATGA TGTTAAACAC
    AATCAAGCTG ATGACGCCTC CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CGCTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATAATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTCATCATCT GTTAAACAAC AACCTAATCT TCTATGACAC AGACCTGGAA
    GCCTGTGGTA TTGACGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CTGGGATCAT TCTTGGTACC ATGTACTGCT TTGTTCACCT GACTCTTCAA
    GAGTTTATCG CAGCCCTTTA TGCACATCTG ATTCTAGACA CTAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAAAAT AAATGAGTCC ATGATTGATT TGCTGAAGAC TGCAGTGGAC
    AAGGCACTAG AGAGTGACAA TGGGCACCTG GACCTTTTTC TTCGCTTCCT TCTCGGTCTG
    TCACTTCAGT CCAATCGACG AATCTTACGA GGTCTGTTGA CACCGCAAGA CCAAGATGAC
    CAAAGCACAG AGAAAATACT TCAGTATATC AAGCAGAAGT TTGATGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGAACAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG CTGACTTTTC ATCTGCCCAC
    TGGTCTGCTT TGGCCTTTGT GTTGTTGACA TCAGATGAGG AGATGGAGGA GTTTGAGCTT
    CAGAAATTCC ATAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTCAT CAAAACCTGC
    AAAAGAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TTTGGCTACA
    GTTCTTGAGT CAGATACCAG TCTAAAAGAG CTGAACATGA GTAATAATAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG TACTGGACTG AAGGATATGA AGTGTGAATT GGAGATACTG
    AGACTTTCAG ACTGCAATAT TACAGAGGAA GGTTATAAAG CACTGGCTTC AGCTCTGAGA
    TCAAATCCTT CACATCTACT AGAGCTAGAC CTCAGAGGAA ATGATCCTGG AGAATCAGGA
    GTAAAGGAGC TTGATGATTT ACTGCAGGAT CCAAACTGTA CACTGAAAAC ACTGAGGTTT
    TTAAGTCCTT CTGCAGAAGA ATCCTGTCAG TTTGTGAATG GGGTTGTGGG TAAAAACCCA
    TTACTCCTAA AAACACTGAA TCTGAATGAT TTCCGATCCA CGCGTAACCA TCCATTTATA
    TATGAAATAG AAGACACACA AGTGAATCAC CTCGCTGCTC TACTGCAGGA TAAACACTGT
    AGAGTCAACA TGCTTATTCT GGGTGGATGC AGTTTTACAG ATGATCAGTG GGAGAGCCTA
    ACTTCAGCTC TGAAATTAAA CCCATCACAC CTGAGAGAAC TGATCCTGAT GTGGAATGGA
    CCGGGAGAGT CTGGAGTGAA AATCCTCAGT GATTTCCTAA TCGAGCCACA ATTAAAGCTG
    GAGAAACTAT GTTTGTGTTA TTGCAGTATT ACAGAGGAAC GGTGTGAGAT CCTGACTTCA
    GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGAACC TCAGTCAGAA TATACCTGGC
    GACTCTGGAA TGAAAAACCT CAGTGATTTA CTGATGAATC CACAATCCAA GCTGGAGAAA
    CTACATCTGT CTCATTGCAG TATCACAGAG GAACAGTGCG AGATCCTGAC TTCAGCTCTG
    AAATCAAACC CATCACACCT GAGAGAACTG GACATAAGTA AGAATCAAAT TAGAAACACT
    GGAGTGAATC TCTTATGTGA CGTACTGAAC AATTCAGACT GCAAACTGGA GACACTGAGA
    CTAAACGACT GTGGCATCAC AGATGTTTCT TCTTTAACTC AGTGTTTGAC GAACACAAAA
    GCTCTGCAGT TTCTAAAAGT GCTTTATCTG AGGGAAAATC ACATTGGAGA CTCAAAGCAG
    GAGCTCAGTG ATGTGGTACG AGACTCAAAC TGTGAACTGA GGTCAGTAAA CAACACAGAC
    TGTGTTGAAA ATTATTATGT TTTATTTAAA CAAAGGGTTT GTCTGAGCAC AAAGTGTGCA
    GTGTGTCTGC GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325237.1
    CDS456..4250
    Translation

    Target ORF information:

    RefSeq Version XM_021469562.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253d23.10 (si:dkey-253d23.10), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469562.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGTCAGAGG AGCAGGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAT ATCAGACTCA 
    TGTGTGTCCG GCTCTGTGTC TGTGAAGAGC GACTGCTCAA AAGATGGTGA TCTCCCGAAC
    TTCAGTGTGG AAAAGCCAAT GTCTGCTAAA AGTGTAATAT CAGACTCATG TGTGTCCGGC
    TCTGTGTCTG TGAAGAGCGA CTGCTCAAAA GATGGTGATC TCCCGAACTT CAGTGTGGAA
    AAACAAACGT CTGCTAAAAG TGTAATATCA GACTCCTGTG TGTCCGGCTC TGTGTTTGTG
    AAGAGTGACT GCTCAAAAGA TGGTGATCTC CCGAACTTCA GCGAGGAAAC ACCGTCATCT
    GGCAAAAGTT TTGAACATAA GACATCAGAC TCAGACTTCC AGACTCACAG GAAACACAAG
    GGGTTTCAAG ACGGTCTTCT GGGGATCTTT CAGGATCTTG AAAACCAGAT AATGAATTAT
    CTGAAGCATG AGCTAGAAAA GTTTAAGAAA ATCTTACAAA AAGAAAACAT AATTGACTTT
    GTAAAGGACT TTAACGAGAA CAGATGCAGC ATCAAAGAAG CAGCTCTCGA TCTCACACTC
    TACTTCCTGA GGGAAATGAA GCAAGAGGAA AGTGCTGATA CTCTAGAAGA CGAACTGATC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGATTCAAG ACTTCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGCTTCTAGA
    CGTCATGAAG CACAAGAGCT ACAGGTTGAA TGCAGACACT TGTTTGAAGC GTCTGAACAA
    GATAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC GGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTTGTGATGT TTCGTCGCCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACCGG CTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAAGAGG AGTACTTCAG AAAAAGACTG ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA AAACATTTTA CAGGAGAAGA GAAATAATGA TGTTAAACAC
    AATCAAGCTG ATGACGCCTC CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CGCTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATAATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTCATCATCT GTTAAACAAC AACCTAATCT TCTATGACAC AGACCTGGAA
    GCCTGTGGTA TTGACGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CTGGGATCAT TCTTGGTACC ATGTACTGCT TTGTTCACCT GACTCTTCAA
    GAGTTTATCG CAGCCCTTTA TGCACATCTG ATTCTAGACA CTAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAAAAT AAATGAGTCC ATGATTGATT TGCTGAAGAC TGCAGTGGAC
    AAGGCACTAG AGAGTGACAA TGGGCACCTG GACCTTTTTC TTCGCTTCCT TCTCGGTCTG
    TCACTTCAGT CCAATCGACG AATCTTACGA GGTCTGTTGA CACCGCAAGA CCAAGATGAC
    CAAAGCACAG AGAAAATACT TCAGTATATC AAGCAGAAGT TTGATGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGAACAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG CTGACTTTTC ATCTGCCCAC
    TGGTCTGCTT TGGCCTTTGT GTTGTTGACA TCAGATGAGG AGATGGAGGA GTTTGAGCTT
    CAGAAATTCC ATAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTCAT CAAAACCTGC
    AAAAGAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TTTGGCTACA
    GTTCTTGAGT CAGATACCAG TCTAAAAGAG CTGAACATGA GTAATAATAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG TACTGGACTG AAGGATATGA AGTGTGAATT GGAGATACTG
    AGACTTTCAG ACTGCAATAT TACAGAGGAA GGTTATAAAG CACTGGCTTC AGCTCTGAGA
    TCAAATCCTT CACATCTACT AGAGCTAGAC CTCAGAGGAA ATGATCCTGG AGAATCAGGA
    GTAAAGGAGC TTGATGATTT ACTGCAGGAT CCAAACTGTA CACTGAAAAC ACTGAGGTTT
    TTAAGTCCTT CTGCAGAAGA ATCCTGTCAG TTTGTGAATG GGGTTGTGGG TAAAAACCCA
    TTACTCCTAA AAACACTGAA TCTGAATGAT TTCCGATCCA CGCGTAACCA TCCATTTATA
    TATGAAATAG AAGACACACA AGTGAATCAC CTCGCTGCTC TACTGCAGGA TAAACACTGT
    AGAGTCAACA TGCTTATTCT GGGTGGATGC AGTTTTACAG ATGATCAGTG GGAGAGCCTA
    ACTTCAGCTC TGAAATTAAA CCCATCACAC CTGAGAGAAC TGATCCTGAT GTGGAATGGA
    CCGGGAGAGT CTGGAGTGAA AATCCTCAGT GATTTCCTAA TCGAGCCACA ATTAAAGCTG
    GAGAAACTAT GTTTGTGTTA TTGCAGTATT ACAGAGGAAC GGTGTGAGAT CCTGACTTCA
    GCTCTGAAAT CAAACCCATC ACACCTGAGA GAACTGAACC TCAGTCAGAA TATACCTGGC
    GACTCTGGAA TGAAAAACCT CAGTGATTTA CTGATGAATC CACAATCCAA GCTGGAGAAA
    CTACATCTGT CTCATTGCAG TATCACAGAG GAACAGTGCG AGATCCTGAC TTCAGCTCTG
    AAATCAAACC CATCACACCT GAGAGAACTG GACATAAGTA AGAATCAAAT TAGAAACACT
    GGAGTGAATC TCTTATGTGA CGTACTGAAC AATTCAGACT GCAAACTGGA GACACTGAGA
    CTAAACGACT GTGGCATCAC AGATGTTTCT TCTTTAACTC AGTGTTTGAC GAACACAAAA
    GCTCTGCAGT TTCTAAAAGT GCTTTATCTG AGGGAAAATC ACATTGGAGA CTCAAAGCAG
    GAGCTCAGTG ATGTGGTACG AGACTCAAAC TGTGAACTGA GGTCAGTAAA CAACACAGAC
    TGTGTTGAAA ATTATTATGT TTTATTTAAA CAAAGGGTTT GTCTGAGCAC AAAGTGTGCA
    GTGTGTCTGC GGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa151328
    Clone ID Related Accession (Same CDS sequence) XM_021469563.1
    Accession Version XM_021469563.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3192bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NLR family CARD domain-containing protein 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCAGAGG AGCAGGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAT ATCAGACTCA 
    TGTGTGTCCG GCTCTGTGTC TGTGAAGAGC GACTGCTCAA AAGATGGTGA TCTCCCGAAC
    TTCAGTGTGG AAAAGCCAAT GTCTGCTAAA AGTGTAATAT CAGACTCATG TGTGTCCGGC
    TCTGTGTCTG TGAAGAGCGA CTGCTCAAAA GATGGTGATC TCCCGAACTT CAGTGTGGAA
    AAACAAACGT CTGCTAAAAG TGTAATATCA GACTCCTGTG TGTCCGGCTC TGTGTTTGTG
    AAGAGTGACT GCTCAAAAGA TGGTGATCTC CCGAACTTCA GCGAGGAAAC ACCGTCATCT
    GGCAAAAGTT TTGAACATAA GACATCAGAC TCAGACTTCC AGACTCACAG GAAACACAAG
    GGGTTTCAAG ACGGTCTTCT GGGGATCTTT CAGGATCTTG AAAACCAGAT AATGAATTAT
    CTGAAGCATG AGCTAGAAAA GTTTAAGAAA ATCTTACAAA AAGAAAACAT AATTGACTTT
    GTAAAGGACT TTAACGAGAA CAGATGCAGC ATCAAAGAAG CAGCTCTCGA TCTCACACTC
    TACTTCCTGA GGGAAATGAA GCAAGAGGAA AGTGCTGATA CTCTAGAAGA CGAACTGATC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGATTCAAG ACTTCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGCTTCTAGA
    CGTCATGAAG CACAAGAGCT ACAGGTTGAA TGCAGACACT TGTTTGAAGC GTCTGAACAA
    GATAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC GGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTTGTGATGT TTCGTCGCCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACCGG CTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAAGAGG AGTACTTCAG AAAAAGACTG ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA AAACATTTTA CAGGAGAAGA GAAATAATGA TGTTAAACAC
    AATCAAGCTG ATGACGCCTC CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CGCTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATAATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTCATCATCT GTTAAACAAC AACCTAATCT TCTATGACAC AGACCTGGAA
    GCCTGTGGTA TTGACGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CTGGGATCAT TCTTGGTACC ATGTACTGCT TTGTTCACCT GACTCTTCAA
    GAGTTTATCG CAGCCCTTTA TGCACATCTG ATTCTAGACA CTAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAAAAT AAATGAGTCC ATGATTGATT TGCTGAAGAC TGCAGTGGAC
    AAGGCACTAG AGAGTGACAA TGGGCACCTG GACCTTTTTC TTCGCTTCCT TCTCGGTCTG
    TCACTTCAGT CCAATCGACG AATCTTACGA GGTCTGTTGA CACCGCAAGA CCAAGATGAC
    CAAAGCACAG AGAAAATACT TCAGTATATC AAGCAGAAGT TTGATGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGAACAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG CTGACTTTTC ATCTGCCCAC
    TGGTCTGCTT TGGCCTTTGT GTTGTTGACA TCAGATGAGG AGATGGAGGA GTTTGAGCTT
    CAGAAATTCC ATAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTCAT CAAAACCTGC
    AAAAGAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TTTGGCTACA
    GTTCTTGAGT CAGATACCAG TCTAAAAGAG CTGAACATGA GTAATAATAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG TACTGGACTG AAGGATATGA AGTGTGAATT GGAGATACTG
    AGACTTTCAG ACTGCAATAT TACAGAGGAA GGTTATAAAG CACTGGCTTC AGCTCTGAGA
    TCAAATCCTT CACATCTACT AGAGCTAGAC CTCAGAGGAA ATGATCCTGG AGAATCAGGA
    GTAAAGGAGC TTGATGATTT ACTGCAGGAT CCAAACTGTA CACTGAAAAC ACTGAGGTTT
    TTAAGTCCTT CTGCAGAAGA ATCCTGTCAG TTTGTGAATG GGGTTGTGGG TAAAAACCCA
    TTACTCCTAA AAACACTGAA TCTGAATGAT TTCCGATCCA CGCGTAACCA TCCATTTATA
    TATGAAATAG AAGACACACA AGTGAATCAC CTCGCTGCTC TACTGCAGGA TAAACACTGT
    AGAGTCAACA TGCTTATTCT GGGTGGATGC AGTTTTACAG ATGATCAGTG GGAGAGCCTA
    ACTTCAGCTC TGAAATTAAA CCCATCACAC CTGAGAGAAC TGATCCTGAT GTGGAATGGA
    CCGGGAGAGT CTGGAGTGAA AATCCTCAGT GATTTCCTAA TCGAGCCACA ATTAAAGCTG
    GAGAAACTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021325238.1
    CDS456..3647
    Translation

    Target ORF information:

    RefSeq Version XM_021469563.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:dkey-253d23.10 (si:dkey-253d23.10), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021469563.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCAGAGG AGCAGGGAAA GATGAGTCTC TCAGAGAAAC ACAGTGTAAT ATCAGACTCA 
    TGTGTGTCCG GCTCTGTGTC TGTGAAGAGC GACTGCTCAA AAGATGGTGA TCTCCCGAAC
    TTCAGTGTGG AAAAGCCAAT GTCTGCTAAA AGTGTAATAT CAGACTCATG TGTGTCCGGC
    TCTGTGTCTG TGAAGAGCGA CTGCTCAAAA GATGGTGATC TCCCGAACTT CAGTGTGGAA
    AAACAAACGT CTGCTAAAAG TGTAATATCA GACTCCTGTG TGTCCGGCTC TGTGTTTGTG
    AAGAGTGACT GCTCAAAAGA TGGTGATCTC CCGAACTTCA GCGAGGAAAC ACCGTCATCT
    GGCAAAAGTT TTGAACATAA GACATCAGAC TCAGACTTCC AGACTCACAG GAAACACAAG
    GGGTTTCAAG ACGGTCTTCT GGGGATCTTT CAGGATCTTG AAAACCAGAT AATGAATTAT
    CTGAAGCATG AGCTAGAAAA GTTTAAGAAA ATCTTACAAA AAGAAAACAT AATTGACTTT
    GTAAAGGACT TTAACGAGAA CAGATGCAGC ATCAAAGAAG CAGCTCTCGA TCTCACACTC
    TACTTCCTGA GGGAAATGAA GCAAGAGGAA AGTGCTGATA CTCTAGAAGA CGAACTGATC
    TTCATTCATC AGCTGAAATG TGGCCTACAG AAGAAGTATC AGTGTGTGTT TGAAGGAATT
    GCACAGCAAG GAGATTCAAG ACTTCTGAAG AACATCTACA CAGATCTCTA TATCACTCAG
    GGTGGCAGTG AACAGGTCAA TACTGAACAT GAGGTCAGAC AGATTGAAGT TGCTTCTAGA
    CGTCATGAAG CACAAGAGCT ACAGGTTGAA TGCAGACACT TGTTTGAAGC GTCTGAACAA
    GATAAGCAGA TCAGAACTGT ACTGACAAAA GGAGTCGCTG GCATCGGGAA ATCAGTCTCT
    GTGCAAAAGT TTGTTCTGGA TTGGGCTGAA GGAAAAGAAA ATCAAGAGAT CAGCTTCATA
    TTTCCTCTTC CATTCAGAGA GATGAACTTA AAGGAGAAAG AAACACTCAG TTTGATGGAG
    CTTATAACTC AGTTTTTCCC GGAGACAAAA GGACTGAACC TTACAAGAAG GAATCGATTC
    AAAGTCCTGT TCATCCTTGA TGGATTGGAT GAATGTCGTC TTCCTCTGAA CTTTGCTGGT
    AATGAGACGT GTTGTGATGT TTCGTCGCCA GCCTCTCTGG ATGTTCTCCT GACGAACCTC
    ATCAAGGGAA ATCTGCTTCC TTCTGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCTGCC
    AGTAAGATTC CTCCTGACTG TATCGACCGG CTGACAGAGA TACGAGGATT CAATGATGCA
    CAAAAAGAGG AGTACTTCAG AAAAAGACTG ACAGATGAGA ATCAGGCCAA AGAAATCATT
    GATCATGTTA AACAATCAAA GAGTCTCTTC ATCATGTGCC ACATCCCAGT CTTCTGCTGG
    ATTTCAGCCA CTGTTCTCCA AAACATTTTA CAGGAGAAGA GAAATAATGA TGTTAAACAC
    AATCAAGCTG ATGACGCCTC CAAAACACTG CAGGAATCAA ACACTGAAGA CACTCCCAAG
    ACTCTGACAC AGATGTACAC ACACTTTCTG CGCTTTCAGA TCCAGCAGAG CAGACGAAAG
    TATAATGGAG AATACACACC AGATGTGTCC TGGGATAAAG ACGCCATCCT TTCACTGGGG
    AAACTGGCAT TTCATCATCT GTTAAACAAC AACCTAATCT TCTATGACAC AGACCTGGAA
    GCCTGTGGTA TTGACGTCTA TAAGGCCTCA GTTTACTCAG GCATGTGTAC CCAGATCTTT
    AAGGAGGAAA CTGGGATCAT TCTTGGTACC ATGTACTGCT TTGTTCACCT GACTCTTCAA
    GAGTTTATCG CAGCCCTTTA TGCACATCTG ATTCTAGACA CTAACAAGAA AAGTGTGTTT
    GATCAAGACT CTAAAAAAAT AAATGAGTCC ATGATTGATT TGCTGAAGAC TGCAGTGGAC
    AAGGCACTAG AGAGTGACAA TGGGCACCTG GACCTTTTTC TTCGCTTCCT TCTCGGTCTG
    TCACTTCAGT CCAATCGACG AATCTTACGA GGTCTGTTGA CACCGCAAGA CCAAGATGAC
    CAAAGCACAG AGAAAATACT TCAGTATATC AAGCAGAAGT TTGATGCGAA TCTTTCTCCA
    GAGAGATCCA TCAATCTGTT CTACTGTCTG AATGAACTGA ACGAACAAAC TCTGGTGAAA
    GAGATTCAGA CTCACCTTAG CAAAGGAAGT CTCTCATCTG CTGACTTTTC ATCTGCCCAC
    TGGTCTGCTT TGGCCTTTGT GTTGTTGACA TCAGATGAGG AGATGGAGGA GTTTGAGCTT
    CAGAAATTCC ATAAATCAGA CGAGTGTCTC ATCAGACTAT CAGCAGTCAT CAAAACCTGC
    AAAAGAGCTC TGTTAAATGA TTGTAACTTA ACAGACAGAA GCTGTTCAGC TTTGGCTACA
    GTTCTTGAGT CAGATACCAG TCTAAAAGAG CTGAACATGA GTAATAATAA TCTGCAGGAT
    TCAGGAGTGA AGCTGCTCTG TACTGGACTG AAGGATATGA AGTGTGAATT GGAGATACTG
    AGACTTTCAG ACTGCAATAT TACAGAGGAA GGTTATAAAG CACTGGCTTC AGCTCTGAGA
    TCAAATCCTT CACATCTACT AGAGCTAGAC CTCAGAGGAA ATGATCCTGG AGAATCAGGA
    GTAAAGGAGC TTGATGATTT ACTGCAGGAT CCAAACTGTA CACTGAAAAC ACTGAGGTTT
    TTAAGTCCTT CTGCAGAAGA ATCCTGTCAG TTTGTGAATG GGGTTGTGGG TAAAAACCCA
    TTACTCCTAA AAACACTGAA TCTGAATGAT TTCCGATCCA CGCGTAACCA TCCATTTATA
    TATGAAATAG AAGACACACA AGTGAATCAC CTCGCTGCTC TACTGCAGGA TAAACACTGT
    AGAGTCAACA TGCTTATTCT GGGTGGATGC AGTTTTACAG ATGATCAGTG GGAGAGCCTA
    ACTTCAGCTC TGAAATTAAA CCCATCACAC CTGAGAGAAC TGATCCTGAT GTGGAATGGA
    CCGGGAGAGT CTGGAGTGAA AATCCTCAGT GATTTCCTAA TCGAGCCACA ATTAAAGCTG
    GAGAAACTAT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.