si:ch211-214b16.3 cDNA ORF clone, Danio rerio(zebrafish)

The following si:ch211-214b16.3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:ch211-214b16.3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa08944 XM_021474776.1
Latest version!
Danio rerio si:ch211-214b16.3 (si:ch211-214b16.3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa141856 XM_021474772.1
Latest version!
Danio rerio si:ch211-214b16.3 (si:ch211-214b16.3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa141857 XM_021474773.1
Latest version!
Danio rerio si:ch211-214b16.3 (si:ch211-214b16.3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa141858 XM_021474774.1
Latest version!
Danio rerio si:ch211-214b16.3 (si:ch211-214b16.3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa141859 XM_021474775.1
Latest version!
Danio rerio si:ch211-214b16.3 (si:ch211-214b16.3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa44300 XM_021474771.1
Latest version!
Danio rerio si:ch211-214b16.3 (si:ch211-214b16.3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
ODa58170 XM_017354691.1
Latest version!
Danio rerio si:ch211-214b16.3 (si:ch211-214b16.3), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa08944
    Clone ID Related Accession (Same CDS sequence) XM_021474776.1
    Accession Version XM_021474776.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4470bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 12 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAAGGA AAGCTAAACA TCCATGTGAA ATGAGTGTGT TTGAAGAGAG AGAGAGGGAG 
    GCTTTCATTC AGACTGTGAG AGCAGTTTCT CCATTATCTA GCTGTGTGTC TATGATGTGT
    AACCACTCCA TTGGTCTCCC CCCTGATCGC AGTGATGGAG CTGTTAGCTC TGACTTTGTC
    TGGGAAAGGA CCAGATCTCC ATTATCTAGC TGTGTGTCTA TGAAGAGCAA CAACTCTATG
    GGTCTCCCCC CTGATCTCAG TGATGGAGCT GTCAACTCTG AGTTCCTCAA GAGAAAGAGA
    GAAGAGTCTT CATTGTCTAG CAGCATGTTT ATAAAGAGTG ACCACCCCTT TGGTCTCCCC
    CTTTATCTCA GTGATAGAGC TGTGAACTCT GACTCTGTCT GGGAAAGGAC CAGATCTCCA
    TTATCTAGCT GTGTGTCTAT GAAGAGCAAC AACTCTATGG GTCTCCCCCC TGATCTCAGT
    GATGGAGCTG TCAACTCTGA GTTCCTCAAG AGAAAGAGAG AAGAGTCTTC ATTGTCTAGC
    AGCATGTTTA TAAAGAGTGA CCACCCCTTT GGTCTCCCCC TTTATCTCAG TGATAGAGCT
    GTGAACTCTG ACTCTGTCTG GGAAAGGACC AGATCTCCAT TATCTAGCTG TGTGTCTATG
    AAGAGCAACA ACTCTATGGG TCTCCCCCCT GATCTCAGTG ATGGAGCTGT CAACTCTGAG
    TTCCTCAAGA GAAAGAGAGA AGAGTCTTCA TTGTCTAGCA GCATGTTTAT AAAGAGTGAC
    CACCCCTTTG GTCTCCCCCT TTATCTCAGT GATAGAGCTG TGAACTCTGA CTCTGTCTGG
    AAAAGGACCA GATCTCCATT ATCTAGCTGT GTGTCTATGA AGAGCAACAA CTCTATGGGT
    CTCCCCCCTG ATCTCAGTGA TGGAGCTGTC AACTCTGAGT TCCTCAAGAG AAAGAGAGAA
    GAGTCTTCAT TGTCTAGCAG CATGTCTATG AAGAGTGACC ACTACATTGA TCTCCCCCCA
    TGTAAACTCA TCAGCAATAG AAAGAGAGAG TCTTCTCCAT TATCTAGCTG TGTGTCTATG
    AAGAGTAATC ACTCCATGAG TCTCCCCCCT TATCTCAGTG ATGGAGCTGT GAACTCTGAC
    TCTGTATCTC CCTACTATCA GAAACACATC AGTCATTTTA AAACAGAGGC ATGCCTGATG
    ATTGATTCTC AACAACTAGT GCATGATGAT CTCCAGAGAG TCAAAGACCA GCACAGATTC
    ATCATGAAGA TCAAGTACGA GAGCTTATTT GAGGGAGTGA AACTCCAGCA GAATCAAACC
    CTCCTGAACA GCATTTACAC ACAGCTGTAC ATCATAGAGG GAGAGAGTGA AGGAGTGAAT
    GAAGAACATG AGGTGCTGCA GATGGAGAAG AGATACAAGA CTCTCCAAGA CACTCCAATC
    AACTGCAATG ACATCTTTAA CCCTTCAGCT GAAGCAGGAG ATGAGGAGAA GAGCAGAGAG
    GAGAAAGAGC AGATCAAGAC TGTTCTCACT AAAGGCATCG CTGGAATTGG AAAAACCGTC
    TCTGTGCAGA AGTTCATTCT GGACTGGGCC GAGGGAAAAG CCAATCAGGA TGTAGATTTC
    ATCTTTGTGC TTCCGTTTCG AGAGCTGAAC TTGATTAAAG ATCATCAGTA CAGTCTTCAC
    ACACTTCTGC TGGACTTTCA TCCTGAACTT CAGCATCTGG ACTCACAGAT TTATGAGGAG
    TGTAAAGTCG TGTTCATCTT TGATGGTCTG GATGAAAGCA GAATCACACT GATGTTTTCA
    GACTGTCAGA AAGTTTCTGA TGTGACTGAA GCTTCATCAG TGAGTGTGTT GATGTCAAAC
    CTCATGAAAG GAGATCTGCT TCCCTCCGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCG
    GCCAATCAGA TCCCCTCAAA ACACATCAGC CTCGTGACGG AGATTCAGGG CTTCAATGAC
    CCTCAGAAGG AGGAATATTT CAGGAAGAGA ATCAGAGATG AAGATCAAGC CAGCAGAATC
    ATCTCCCACA TCAGAAGAGC AAGAAGCCTC CACATCATGT GCCACATACC CGTCTTCTGC
    TGGATCTCAG CCACTGTGCT TCAAACCATC CTGAGACAAG ATCTGAGTGC AGAAATCCCT
    CAAACTCTGA CTGAGATGTA CATTCACTTC CTCCTCATTC AGACACACAT GAGAAAGCAG
    AAGTATGAAG AGAGAGATCC AGAGAAACTC CTGCGGTTCA ACAGAGACGT GATTGTGAAA
    CTTGCTGAAC TGGCTTTCAA TCAGCTGATG AAGGGCAATG TGATGTTCTA CGAGGAGGAC
    CTGATTGAGA GCAGGATAGA CGTCGCTGAC GCCTCAGTGT ATTCTGGGAT CTGCACTGAG
    ATCTTCAGAG AGGAATCTGT GATTTATCAG AGGAAGGTTT ACAGCTTTGT TCATCTGAGC
    TTTCAGGAGT GTTTTGCAGC ACTGTATGTG TTTTACTGCT ATTTACACAA CAACACTGAG
    GTGATGAAGA TGTTCCTGAC AGAAATATAC AGAACTCAGA GTAAGAAAGT TTCTCTGGAT
    GTGCTTCTGA AAGGAGTGAT GATTGAAGCC TTGAAAAGTA AAACTGGACA CCTGGATCTG
    TTCCTGCGAT TTCTTCATGG CGTATCACTG GAGTCCAATC AGAGACTCTT ACAGGATCTG
    CTGAAACACA CAGAGAACGA CCCAGACAGC ATCAAGAAAA TAATCAAAAA CCTCAAACGA
    GGACAAAAAA ACAACGTCAG TCCTGAAAGA TGGATGAATC TCTCACACTG CTTGATGGAG
    ATGAAGGACA ATTCTGTGGT TAAAGAAATG CAAGCATTCT TAAAATCTAA AACACAAAGA
    AAATGTCTTT CTCTTGCACA ATGTTCGACT TTGGCAAACA TGATCCTGAT GTCAGAGGAG
    GTGCTGGATG AGCTAGACAT TAAAAAGTAC AACATTAAAT CAAAAGAAGG TCGATGGAGA
    CTGATACCCG CTGTGAGAAA CTGCAGAAAA GCTCTATTGT CAGAATGTAA TCTTGCAGAT
    CAACACTGTG AAACTGTAGC TTCAGCTCTA CAATCATCAA ACAACCTCTT GAGAGAAGTG
    AATCTGAGTC GCAATGAGCT GCAGGATTCA GGAGTGAAGC TGATCTGTGC TGGACTGAAG
    AGCCCAAACT GTCAACTCAA CGTTCTGAAA TTGTCAGGCT GTAATCTCAC TAAACAGTCC
    TGTGAAACTG TGGCTGTGTC TCTACTTTCA TCAAACTGCC GCCTGAGAGA GCTGGACCTG
    AGTCGCAATG ACCTGCAGGA TTCAGGAGTG AAGCTGATCT GTGCTGGACT GAAAAGTGCA
    AACTGTCAAC TCAACACACT GAGACTGGCA AAATGTAACC TCACTGATCA GTGCTGCGAA
    ACTATATCTT CATTTCTACA ATCATCAAAC TCTGTCTTGA GAGAGCTGGA CATAAGCAAC
    AATCGTCTGC AGGATTCAGG AGTGAAGCTG ATTTTTACTG CAATAAAGAG TGAGAACTGT
    CAACTCAACA TCCTGAGATT GTCAAGTTGT AACCTCACTG ATCAGTGCTG TGAAACTTTG
    GCTTCATCTC TACAATCATC AAACTGTGTC CTGAGAGAGC TGGACCTGAG TAACAATGAG
    CTGCAGGATT CAGGAGTGAA GCTGATCTGT GCTGGATTGA AGAGCCCAAA CTGTCAACTC
    AACACACTGA GACTGTCTTA CTGTATGGTG ACAGAGGAAG GTTGTGCGGC TCTGGCTTCA
    GCTCTGAGCT CAACCTCGTC ACACCTGAGA GAGCTGGATC TGAGCTACAA TCACCCAGGA
    GAAGCAGGAC TCAAACTGCT CTCTGACAAA CTCAAACACC TCCACAAACT CAATCTGGGT
    CATGGAGGGG AATTCAGGAC TATATCAACA CTACACAAAT ACGCCTGTAA ACTCACACTG
    GATCCAAACA CAGCACACAA ACACCTCGTT CTATCGGAGG AGAACAGAAA GGCGACGCGT
    GCGTTTAAGG ATCAGTCTTT TCCTAGTCAT CCAGAAAGAT TTGATAAATT GAATCTTCAG
    GTTGTGAGTA AAGAGAGTCT GACTGGACGC TGTTACTGGG AGACTCAATG GAGCGGATAT
    GCTGAAATAT CAGTGTGTTA TAAAGGAATC AAGAGGAAAG GATGGAGTGA TGACTGTGAG
    TTTGGGCGTA ATGATCAGTC CTGGAGTCTT CATTGCTCTG ATCACTCATT CTCTGTCTGT
    CACAATGATG AACGCACACA CATCTCAGTT CCTCCAACCT TTAGTGGGAC AGTAGGAGTG
    TTTGTGGACG AGTCGAGCGG CTCTCTGTCC TTCTACAGCG TCTCTGATAA ACTCACACAC
    TTACACACAT TCAACACCAC ATTCACCGAC ACACTCCACG CTGGATTTAG ACTTTTTTCA
    AACTCTTCCG TGTCTCTGTG TCACATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330451.1
    CDS160..4629
    Translation

    Target ORF information:

    RefSeq Version XM_021474776.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-214b16.3 (si:ch211-214b16.3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474776.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAAAGGA AAGCTAAACA TCCATGTGAA ATGAGTGTGT TTGAAGAGAG AGAGAGGGAG 
    GCTTTCATTC AGACTGTGAG AGCAGTTTCT CCATTATCTA GCTGTGTGTC TATGATGTGT
    AACCACTCCA TTGGTCTCCC CCCTGATCGC AGTGATGGAG CTGTTAGCTC TGACTTTGTC
    TGGGAAAGGA CCAGATCTCC ATTATCTAGC TGTGTGTCTA TGAAGAGCAA CAACTCTATG
    GGTCTCCCCC CTGATCTCAG TGATGGAGCT GTCAACTCTG AGTTCCTCAA GAGAAAGAGA
    GAAGAGTCTT CATTGTCTAG CAGCATGTTT ATAAAGAGTG ACCACCCCTT TGGTCTCCCC
    CTTTATCTCA GTGATAGAGC TGTGAACTCT GACTCTGTCT GGGAAAGGAC CAGATCTCCA
    TTATCTAGCT GTGTGTCTAT GAAGAGCAAC AACTCTATGG GTCTCCCCCC TGATCTCAGT
    GATGGAGCTG TCAACTCTGA GTTCCTCAAG AGAAAGAGAG AAGAGTCTTC ATTGTCTAGC
    AGCATGTTTA TAAAGAGTGA CCACCCCTTT GGTCTCCCCC TTTATCTCAG TGATAGAGCT
    GTGAACTCTG ACTCTGTCTG GGAAAGGACC AGATCTCCAT TATCTAGCTG TGTGTCTATG
    AAGAGCAACA ACTCTATGGG TCTCCCCCCT GATCTCAGTG ATGGAGCTGT CAACTCTGAG
    TTCCTCAAGA GAAAGAGAGA AGAGTCTTCA TTGTCTAGCA GCATGTTTAT AAAGAGTGAC
    CACCCCTTTG GTCTCCCCCT TTATCTCAGT GATAGAGCTG TGAACTCTGA CTCTGTCTGG
    AAAAGGACCA GATCTCCATT ATCTAGCTGT GTGTCTATGA AGAGCAACAA CTCTATGGGT
    CTCCCCCCTG ATCTCAGTGA TGGAGCTGTC AACTCTGAGT TCCTCAAGAG AAAGAGAGAA
    GAGTCTTCAT TGTCTAGCAG CATGTCTATG AAGAGTGACC ACTACATTGA TCTCCCCCCA
    TGTAAACTCA TCAGCAATAG AAAGAGAGAG TCTTCTCCAT TATCTAGCTG TGTGTCTATG
    AAGAGTAATC ACTCCATGAG TCTCCCCCCT TATCTCAGTG ATGGAGCTGT GAACTCTGAC
    TCTGTATCTC CCTACTATCA GAAACACATC AGTCATTTTA AAACAGAGGC ATGCCTGATG
    ATTGATTCTC AACAACTAGT GCATGATGAT CTCCAGAGAG TCAAAGACCA GCACAGATTC
    ATCATGAAGA TCAAGTACGA GAGCTTATTT GAGGGAGTGA AACTCCAGCA GAATCAAACC
    CTCCTGAACA GCATTTACAC ACAGCTGTAC ATCATAGAGG GAGAGAGTGA AGGAGTGAAT
    GAAGAACATG AGGTGCTGCA GATGGAGAAG AGATACAAGA CTCTCCAAGA CACTCCAATC
    AACTGCAATG ACATCTTTAA CCCTTCAGCT GAAGCAGGAG ATGAGGAGAA GAGCAGAGAG
    GAGAAAGAGC AGATCAAGAC TGTTCTCACT AAAGGCATCG CTGGAATTGG AAAAACCGTC
    TCTGTGCAGA AGTTCATTCT GGACTGGGCC GAGGGAAAAG CCAATCAGGA TGTAGATTTC
    ATCTTTGTGC TTCCGTTTCG AGAGCTGAAC TTGATTAAAG ATCATCAGTA CAGTCTTCAC
    ACACTTCTGC TGGACTTTCA TCCTGAACTT CAGCATCTGG ACTCACAGAT TTATGAGGAG
    TGTAAAGTCG TGTTCATCTT TGATGGTCTG GATGAAAGCA GAATCACACT GATGTTTTCA
    GACTGTCAGA AAGTTTCTGA TGTGACTGAA GCTTCATCAG TGAGTGTGTT GATGTCAAAC
    CTCATGAAAG GAGATCTGCT TCCCTCCGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCG
    GCCAATCAGA TCCCCTCAAA ACACATCAGC CTCGTGACGG AGATTCAGGG CTTCAATGAC
    CCTCAGAAGG AGGAATATTT CAGGAAGAGA ATCAGAGATG AAGATCAAGC CAGCAGAATC
    ATCTCCCACA TCAGAAGAGC AAGAAGCCTC CACATCATGT GCCACATACC CGTCTTCTGC
    TGGATCTCAG CCACTGTGCT TCAAACCATC CTGAGACAAG ATCTGAGTGC AGAAATCCCT
    CAAACTCTGA CTGAGATGTA CATTCACTTC CTCCTCATTC AGACACACAT GAGAAAGCAG
    AAGTATGAAG AGAGAGATCC AGAGAAACTC CTGCGGTTCA ACAGAGACGT GATTGTGAAA
    CTTGCTGAAC TGGCTTTCAA TCAGCTGATG AAGGGCAATG TGATGTTCTA CGAGGAGGAC
    CTGATTGAGA GCAGGATAGA CGTCGCTGAC GCCTCAGTGT ATTCTGGGAT CTGCACTGAG
    ATCTTCAGAG AGGAATCTGT GATTTATCAG AGGAAGGTTT ACAGCTTTGT TCATCTGAGC
    TTTCAGGAGT GTTTTGCAGC ACTGTATGTG TTTTACTGCT ATTTACACAA CAACACTGAG
    GTGATGAAGA TGTTCCTGAC AGAAATATAC AGAACTCAGA GTAAGAAAGT TTCTCTGGAT
    GTGCTTCTGA AAGGAGTGAT GATTGAAGCC TTGAAAAGTA AAACTGGACA CCTGGATCTG
    TTCCTGCGAT TTCTTCATGG CGTATCACTG GAGTCCAATC AGAGACTCTT ACAGGATCTG
    CTGAAACACA CAGAGAACGA CCCAGACAGC ATCAAGAAAA TAATCAAAAA CCTCAAACGA
    GGACAAAAAA ACAACGTCAG TCCTGAAAGA TGGATGAATC TCTCACACTG CTTGATGGAG
    ATGAAGGACA ATTCTGTGGT TAAAGAAATG CAAGCATTCT TAAAATCTAA AACACAAAGA
    AAATGTCTTT CTCTTGCACA ATGTTCGACT TTGGCAAACA TGATCCTGAT GTCAGAGGAG
    GTGCTGGATG AGCTAGACAT TAAAAAGTAC AACATTAAAT CAAAAGAAGG TCGATGGAGA
    CTGATACCCG CTGTGAGAAA CTGCAGAAAA GCTCTATTGT CAGAATGTAA TCTTGCAGAT
    CAACACTGTG AAACTGTAGC TTCAGCTCTA CAATCATCAA ACAACCTCTT GAGAGAAGTG
    AATCTGAGTC GCAATGAGCT GCAGGATTCA GGAGTGAAGC TGATCTGTGC TGGACTGAAG
    AGCCCAAACT GTCAACTCAA CGTTCTGAAA TTGTCAGGCT GTAATCTCAC TAAACAGTCC
    TGTGAAACTG TGGCTGTGTC TCTACTTTCA TCAAACTGCC GCCTGAGAGA GCTGGACCTG
    AGTCGCAATG ACCTGCAGGA TTCAGGAGTG AAGCTGATCT GTGCTGGACT GAAAAGTGCA
    AACTGTCAAC TCAACACACT GAGACTGGCA AAATGTAACC TCACTGATCA GTGCTGCGAA
    ACTATATCTT CATTTCTACA ATCATCAAAC TCTGTCTTGA GAGAGCTGGA CATAAGCAAC
    AATCGTCTGC AGGATTCAGG AGTGAAGCTG ATTTTTACTG CAATAAAGAG TGAGAACTGT
    CAACTCAACA TCCTGAGATT GTCAAGTTGT AACCTCACTG ATCAGTGCTG TGAAACTTTG
    GCTTCATCTC TACAATCATC AAACTGTGTC CTGAGAGAGC TGGACCTGAG TAACAATGAG
    CTGCAGGATT CAGGAGTGAA GCTGATCTGT GCTGGATTGA AGAGCCCAAA CTGTCAACTC
    AACACACTGA GACTGTCTTA CTGTATGGTG ACAGAGGAAG GTTGTGCGGC TCTGGCTTCA
    GCTCTGAGCT CAACCTCGTC ACACCTGAGA GAGCTGGATC TGAGCTACAA TCACCCAGGA
    GAAGCAGGAC TCAAACTGCT CTCTGACAAA CTCAAACACC TCCACAAACT CAATCTGGGT
    CATGGAGGGG AATTCAGGAC TATATCAACA CTACACAAAT ACGCCTGTAA ACTCACACTG
    GATCCAAACA CAGCACACAA ACACCTCGTT CTATCGGAGG AGAACAGAAA GGCGACGCGT
    GCGTTTAAGG ATCAGTCTTT TCCTAGTCAT CCAGAAAGAT TTGATAAATT GAATCTTCAG
    GTTGTGAGTA AAGAGAGTCT GACTGGACGC TGTTACTGGG AGACTCAATG GAGCGGATAT
    GCTGAAATAT CAGTGTGTTA TAAAGGAATC AAGAGGAAAG GATGGAGTGA TGACTGTGAG
    TTTGGGCGTA ATGATCAGTC CTGGAGTCTT CATTGCTCTG ATCACTCATT CTCTGTCTGT
    CACAATGATG AACGCACACA CATCTCAGTT CCTCCAACCT TTAGTGGGAC AGTAGGAGTG
    TTTGTGGACG AGTCGAGCGG CTCTCTGTCC TTCTACAGCG TCTCTGATAA ACTCACACAC
    TTACACACAT TCAACACCAC ATTCACCGAC ACACTCCACG CTGGATTTAG ACTTTTTTCA
    AACTCTTCCG TGTCTCTGTG TCACATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141856
    Clone ID Related Accession (Same CDS sequence) XM_021474772.1
    Accession Version XM_021474772.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4638bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAAAGGA AAGCTAAACA TCCATGTGAA ATGAGTGTGT TTGAAGAGAG AGAGAGGGAG 
    GCTTTCATTC AGACTGTGAG AGCAGTTTCT CCATTATCTA GCTGTGTGTC TATGATGTGT
    AACCACTCCA TTGGTCTCCC CCCTGATCGC AGTGATGGAG CTGTTAGCTC TGACTTTGTC
    TGGGAAAGGA CCAGATCTCC ATTATCTAGC TGTGTGTCTA TGAAGAGCAA CAACTCTATG
    GGTCTCCCCC CTGATCTCAG TGATGGAGCT GTCAACTCTG AGTTCCTCAA GAGAAAGAGA
    GAAGAGTCTT CATTGTCTAG CAGCATGTTT ATAAAGAGTG ACCACCCCTT TGGTCTCCCC
    CTTTATCTCA GTGATAGAGC TGTGAACTCT GACTCTGTCT GGGAAAGGAC CAGATCTCCA
    TTATCTAGCT GTGTGTCTAT GAAGAGCAAC AACTCTATGG GTCTCCCCCC TGATCTCAGT
    GATGGAGCTG TCAACTCTGA GTTCCTCAAG AGAAAGAGAG AAGAGTCTTC ATTGTCTAGC
    AGCATGTTTA TAAAGAGTGA CCACCCCTTT GGTCTCCCCC TTTATCTCAG TGATAGAGCT
    GTGAACTCTG ACTCTGTCTG GGAAAGGACC AGATCTCCAT TATCTAGCTG TGTGTCTATG
    AAGAGCAACA ACTCTATGGG TCTCCCCCCT GATCTCAGTG ATGGAGCTGT CAACTCTGAG
    TTCCTCAAGA GAAAGAGAGA AGAGTCTTCA TTGTCTAGCA GCATGTTTAT AAAGAGTGAC
    CACCCCTTTG GTCTCCCCCT TTATCTCAGT GATAGAGCTG TGAACTCTGA CTCTGTCTGG
    AAAAGGACCA GATCTCCATT ATCTAGCTGT GTGTCTATGA AGAGCAACAA CTCTATGGGT
    CTCCCCCCTG ATCTCAGTGA TGGAGCTGTC AACTCTGAGT TCCTCAAGAG AAAGAGAGAA
    GAGTCTTCAT TGTCTAGCAG CATGTCTATG AAGAGTGACC ACTACATTGA TCTCCCCCCA
    TGTAAACTCA TCAATAGAAA GAGAGAGTCT TCTCCATTAT CTAGCTGTGT GTCTATGAAG
    AGTAATCACT CCATGAGTCT CCCCCCTTAT CTCAGTGATG GAGCTGTGAA CTCTGACTCT
    GTATCTCCCT ACTATCAGAA ACACATCAGT CATTTTAAAA CAGAGGCATG CCTGATGATT
    GATTCTCAAC AACTAGTGCA TGATGATCTC CAGAGAGTCA AAGACCAGCA CAGATTCATC
    ATGAAGATCA AGTACGAGAG CTTATTTGAG GGAGTGAAAC TCCAGCAGAA TCAAACCCTC
    CTGAACAGCA TTTACACACA GCTGTACATC ATAGAGGGAG AGAGTGAAGG AGTGAATGAA
    GAACATGAGG TGCTGCAGAT GGAGAAGAGA TACAAGACTC TCCAAGACAC TCCAATCAAC
    TGCAATGACA TCTTTAACCC TTCAGCTGAA GCAGGAGATG AGGAGAAGAG CAGAGAGGAG
    AAAGAGCAGA TCAAGACTGT TCTCACTAAA GGCATCGCTG GAATTGGAAA AACCGTCTCT
    GTGCAGAAGT TCATTCTGGA CTGGGCCGAG GGAAAAGCCA ATCAGGATGT AGATTTCATC
    TTTGTGCTTC CGTTTCGAGA GCTGAACTTG ATTAAAGATC ATCAGTACAG TCTTCACACA
    CTTCTGCTGG ACTTTCATCC TGAACTTCAG CATCTGGACT CACAGATTTA TGAGGAGTGT
    AAAGTCGTGT TCATCTTTGA TGGTCTGGAT GAAAGCAGAA TCACACTGAT GTTTTCAGAC
    TGTCAGAAAG TTTCTGATGT GACTGAAGCT TCATCAGTGA GTGTGTTGAT GTCAAACCTC
    ATGAAAGGAG ATCTGCTTCC CTCCGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCGGCC
    AATCAGATCC CCTCAAAACA CATCAGCCTC GTGACGGAGA TTCAGGGCTT CAATGACCCT
    CAGAAGGAGG AATATTTCAG GAAGAGAATC AGAGATGAAG ATCAAGCCAG CAGAATCATC
    TCCCACATCA GAAGAGCAAG AAGCCTCCAC ATCATGTGCC ACATACCCGT CTTCTGCTGG
    ATCTCAGCCA CTGTGCTTCA AACCATCCTG AGACAAGATC TGAGTGCAGA AATCCCTCAA
    ACTCTGACTG AGATGTACAT TCACTTCCTC CTCATTCAGA CACACATGAG AAAGCAGAAG
    TATGAAGAGA GAGATCCAGA GAAACTCCTG CGGTTCAACA GAGACGTGAT TGTGAAACTT
    GCTGAACTGG CTTTCAATCA GCTGATGAAG GGCAATGTGA TGTTCTACGA GGAGGACCTG
    ATTGAGAGCA GGATAGACGT CGCTGACGCC TCAGTGTATT CTGGGATCTG CACTGAGATC
    TTCAGAGAGG AATCTGTGAT TTATCAGAGG AAGGTTTACA GCTTTGTTCA TCTGAGCTTT
    CAGGAGTGTT TTGCAGCACT GTATGTGTTT TACTGCTATT TACACAACAA CACTGAGGTG
    ATGAAGATGT TCCTGACAGA AATATACAGA ACTCAGAGTA AGAAAGTTTC TCTGGATGTG
    CTTCTGAAAG GAGTGATGAT TGAAGCCTTG AAAAGTAAAA CTGGACACCT GGATCTGTTC
    CTGCGATTTC TTCATGGCGT ATCACTGGAG TCCAATCAGA GACTCTTACA GGATCTGCTG
    AAACACACAG AGAACGACCC AGACAGCATC AAGAAAATAA TCAAAAACCT CAAACGAGGA
    CAAAAAAACA ACGTCAGTCC TGAAAGATGG ATGAATCTCT CACACTGCTT GATGGAGATG
    AAGGACAATT CTGTGGTTAA AGAAATGCAA GCATTCTTAA AATCTAAAAC ACAAAGAAAA
    TGTCTTTCTC TTGCACAATG TTCGACTTTG GCAAACATGA TCCTGATGTC AGAGGAGGTG
    CTGGATGAGC TAGACATTAA AAAGTACAAC ATTAAATCAA AAGAAGGTCG ATGGAGACTG
    ATACCCGCTG TGAGAAACTG CAGAAAAGCT CTATTGTCAG AATGTAATCT TGCAGATCAA
    CACTGTGAAA CTGTAGCTTC AGCTCTACAA TCATCAAACA ACCTCTTGAG AGAAGTGAAT
    CTGAGTCGCA ATGAGCTGCA GGATTCAGGA GTGAAGCTGA TCTGTGCTGG ACTGAAGAGC
    CCAAACTGTC AACTCAACGT TCTGAAATTG TCAGGCTGTA ATCTCACTAA ACAGTCCTGT
    GAAACTGTGG CTGTGTCTCT ACTTTCATCA AACTGCCGCC TGAGAGAGCT GGACCTGAGT
    CGCAATGACC TGCAGGATTC AGGAGTGAAG CTGATCTGTG CTGGACTGAA AAGTGCAAAC
    TGTCAACTCA ACACACTGAG ACTGGCAAAA TGTAACCTCA CTGATCAGTG CTGCGAAACT
    ATATCTTCAT TTCTACAATC ATCAAACTCT GTCTTGAGAG AGCTGGACAT AAGCAACAAT
    CGTCTGCAGG ATTCAGGAGT GAAGCTGATT TTTACTGCAA TAAAGAGTGA GAACTGTCAA
    CTCAACATCC TGAGATTGTC AAGTTGTAAC CTCACTGATC AGTGCTGTGA AACTTTGGCT
    TCATCTCTAC AATCATCAAA CTGTGTCCTG AGAGAGCTGG ACCTGAGTAA CAATGAGCTG
    CAGGATTCAG GAGTGAAGCT GATCTGTGCT GGATTGAAGA GCCCAAACTG TCAACTCAAC
    ACACTGAGAT TGTCAGGCTG TAATCTCACT GATCAGTGCT GTGAAACTGT GGCTTCATCT
    CTACAATTAT CCAACTGCCC TCTGAGAGAG CTGGACCTGA GTCACAATGA TCTTCAGGGT
    TCAAGAGTGA AGCTGATCTG TGCTGGACTG AAGAGCCCAA ACTGTCAACT CAACACACTG
    AGATTATCTT ACTGTTTGGT GTCAGAGGAA GGTTGTGCAG CTCTGGCTTC AGCTCTGAGC
    TCAAACCCGT CACACCTGAG AGAGCTGGAT CTGAGCTACA ATCACCCAGG AGAAGCAGGA
    CTCAAACTGC TCTCTGATCA GCTCAAACAC CTCCACAAAC TCAATGTGGA TCATGGAGGA
    GAATTCAGGA CTGTATCAAC ACTACACAAA TACGCCTGTG AACTCACACT GGATCCAAAC
    ACAGCACACA CACGCCTCGC TCTGTCTGAG GAGAACAGAA AGGCGACGCG TGTGAAGGAG
    AGTCAGTCTT ATCCTCGTCA TCCAGACAGA TTTGATAAGT TTCATCAGGT TATGAGTAAA
    GAGAGTCTGA CTGGACGCTG TTACTGGGAG ACTCAATGGA GCGGATATGC TGAAATATCA
    GTTTGTTATA AAGGAATCAA GAGGAAAGGA CGGAGTGACT GTCGGTTTGG ATGTAATGAT
    CAGTCCTGGA GACTTGATTG CTCTGATCAC TCATTCTCTG TCTGTCACAA TAATGAACTC
    ACACACATCT CAGCTCGTCC AGACTCCAGC AGGACAGTAG GAGTGTTTGT GGACGAGTCG
    AGCGGCTCTC TGTCCTTCTA CAGTGTCTCT GATAAACTCA CACACTTACA CACATTCAAC
    ACCACATTCA CCGACACACT CCACGCTGGA TTTAGACTTG GTTATAAATC TTCAGTGTCT
    CTGTGTCGCA TTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330447.1
    CDS160..4797
    Translation

    Target ORF information:

    RefSeq Version XM_021474772.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-214b16.3 (si:ch211-214b16.3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474772.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAAAGGA AAGCTAAACA TCCATGTGAA ATGAGTGTGT TTGAAGAGAG AGAGAGGGAG 
    GCTTTCATTC AGACTGTGAG AGCAGTTTCT CCATTATCTA GCTGTGTGTC TATGATGTGT
    AACCACTCCA TTGGTCTCCC CCCTGATCGC AGTGATGGAG CTGTTAGCTC TGACTTTGTC
    TGGGAAAGGA CCAGATCTCC ATTATCTAGC TGTGTGTCTA TGAAGAGCAA CAACTCTATG
    GGTCTCCCCC CTGATCTCAG TGATGGAGCT GTCAACTCTG AGTTCCTCAA GAGAAAGAGA
    GAAGAGTCTT CATTGTCTAG CAGCATGTTT ATAAAGAGTG ACCACCCCTT TGGTCTCCCC
    CTTTATCTCA GTGATAGAGC TGTGAACTCT GACTCTGTCT GGGAAAGGAC CAGATCTCCA
    TTATCTAGCT GTGTGTCTAT GAAGAGCAAC AACTCTATGG GTCTCCCCCC TGATCTCAGT
    GATGGAGCTG TCAACTCTGA GTTCCTCAAG AGAAAGAGAG AAGAGTCTTC ATTGTCTAGC
    AGCATGTTTA TAAAGAGTGA CCACCCCTTT GGTCTCCCCC TTTATCTCAG TGATAGAGCT
    GTGAACTCTG ACTCTGTCTG GGAAAGGACC AGATCTCCAT TATCTAGCTG TGTGTCTATG
    AAGAGCAACA ACTCTATGGG TCTCCCCCCT GATCTCAGTG ATGGAGCTGT CAACTCTGAG
    TTCCTCAAGA GAAAGAGAGA AGAGTCTTCA TTGTCTAGCA GCATGTTTAT AAAGAGTGAC
    CACCCCTTTG GTCTCCCCCT TTATCTCAGT GATAGAGCTG TGAACTCTGA CTCTGTCTGG
    AAAAGGACCA GATCTCCATT ATCTAGCTGT GTGTCTATGA AGAGCAACAA CTCTATGGGT
    CTCCCCCCTG ATCTCAGTGA TGGAGCTGTC AACTCTGAGT TCCTCAAGAG AAAGAGAGAA
    GAGTCTTCAT TGTCTAGCAG CATGTCTATG AAGAGTGACC ACTACATTGA TCTCCCCCCA
    TGTAAACTCA TCAATAGAAA GAGAGAGTCT TCTCCATTAT CTAGCTGTGT GTCTATGAAG
    AGTAATCACT CCATGAGTCT CCCCCCTTAT CTCAGTGATG GAGCTGTGAA CTCTGACTCT
    GTATCTCCCT ACTATCAGAA ACACATCAGT CATTTTAAAA CAGAGGCATG CCTGATGATT
    GATTCTCAAC AACTAGTGCA TGATGATCTC CAGAGAGTCA AAGACCAGCA CAGATTCATC
    ATGAAGATCA AGTACGAGAG CTTATTTGAG GGAGTGAAAC TCCAGCAGAA TCAAACCCTC
    CTGAACAGCA TTTACACACA GCTGTACATC ATAGAGGGAG AGAGTGAAGG AGTGAATGAA
    GAACATGAGG TGCTGCAGAT GGAGAAGAGA TACAAGACTC TCCAAGACAC TCCAATCAAC
    TGCAATGACA TCTTTAACCC TTCAGCTGAA GCAGGAGATG AGGAGAAGAG CAGAGAGGAG
    AAAGAGCAGA TCAAGACTGT TCTCACTAAA GGCATCGCTG GAATTGGAAA AACCGTCTCT
    GTGCAGAAGT TCATTCTGGA CTGGGCCGAG GGAAAAGCCA ATCAGGATGT AGATTTCATC
    TTTGTGCTTC CGTTTCGAGA GCTGAACTTG ATTAAAGATC ATCAGTACAG TCTTCACACA
    CTTCTGCTGG ACTTTCATCC TGAACTTCAG CATCTGGACT CACAGATTTA TGAGGAGTGT
    AAAGTCGTGT TCATCTTTGA TGGTCTGGAT GAAAGCAGAA TCACACTGAT GTTTTCAGAC
    TGTCAGAAAG TTTCTGATGT GACTGAAGCT TCATCAGTGA GTGTGTTGAT GTCAAACCTC
    ATGAAAGGAG ATCTGCTTCC CTCCGCTCTC ATCTGGATCA CCAGCAGACC AGCAGCGGCC
    AATCAGATCC CCTCAAAACA CATCAGCCTC GTGACGGAGA TTCAGGGCTT CAATGACCCT
    CAGAAGGAGG AATATTTCAG GAAGAGAATC AGAGATGAAG ATCAAGCCAG CAGAATCATC
    TCCCACATCA GAAGAGCAAG AAGCCTCCAC ATCATGTGCC ACATACCCGT CTTCTGCTGG
    ATCTCAGCCA CTGTGCTTCA AACCATCCTG AGACAAGATC TGAGTGCAGA AATCCCTCAA
    ACTCTGACTG AGATGTACAT TCACTTCCTC CTCATTCAGA CACACATGAG AAAGCAGAAG
    TATGAAGAGA GAGATCCAGA GAAACTCCTG CGGTTCAACA GAGACGTGAT TGTGAAACTT
    GCTGAACTGG CTTTCAATCA GCTGATGAAG GGCAATGTGA TGTTCTACGA GGAGGACCTG
    ATTGAGAGCA GGATAGACGT CGCTGACGCC TCAGTGTATT CTGGGATCTG CACTGAGATC
    TTCAGAGAGG AATCTGTGAT TTATCAGAGG AAGGTTTACA GCTTTGTTCA TCTGAGCTTT
    CAGGAGTGTT TTGCAGCACT GTATGTGTTT TACTGCTATT TACACAACAA CACTGAGGTG
    ATGAAGATGT TCCTGACAGA AATATACAGA ACTCAGAGTA AGAAAGTTTC TCTGGATGTG
    CTTCTGAAAG GAGTGATGAT TGAAGCCTTG AAAAGTAAAA CTGGACACCT GGATCTGTTC
    CTGCGATTTC TTCATGGCGT ATCACTGGAG TCCAATCAGA GACTCTTACA GGATCTGCTG
    AAACACACAG AGAACGACCC AGACAGCATC AAGAAAATAA TCAAAAACCT CAAACGAGGA
    CAAAAAAACA ACGTCAGTCC TGAAAGATGG ATGAATCTCT CACACTGCTT GATGGAGATG
    AAGGACAATT CTGTGGTTAA AGAAATGCAA GCATTCTTAA AATCTAAAAC ACAAAGAAAA
    TGTCTTTCTC TTGCACAATG TTCGACTTTG GCAAACATGA TCCTGATGTC AGAGGAGGTG
    CTGGATGAGC TAGACATTAA AAAGTACAAC ATTAAATCAA AAGAAGGTCG ATGGAGACTG
    ATACCCGCTG TGAGAAACTG CAGAAAAGCT CTATTGTCAG AATGTAATCT TGCAGATCAA
    CACTGTGAAA CTGTAGCTTC AGCTCTACAA TCATCAAACA ACCTCTTGAG AGAAGTGAAT
    CTGAGTCGCA ATGAGCTGCA GGATTCAGGA GTGAAGCTGA TCTGTGCTGG ACTGAAGAGC
    CCAAACTGTC AACTCAACGT TCTGAAATTG TCAGGCTGTA ATCTCACTAA ACAGTCCTGT
    GAAACTGTGG CTGTGTCTCT ACTTTCATCA AACTGCCGCC TGAGAGAGCT GGACCTGAGT
    CGCAATGACC TGCAGGATTC AGGAGTGAAG CTGATCTGTG CTGGACTGAA AAGTGCAAAC
    TGTCAACTCA ACACACTGAG ACTGGCAAAA TGTAACCTCA CTGATCAGTG CTGCGAAACT
    ATATCTTCAT TTCTACAATC ATCAAACTCT GTCTTGAGAG AGCTGGACAT AAGCAACAAT
    CGTCTGCAGG ATTCAGGAGT GAAGCTGATT TTTACTGCAA TAAAGAGTGA GAACTGTCAA
    CTCAACATCC TGAGATTGTC AAGTTGTAAC CTCACTGATC AGTGCTGTGA AACTTTGGCT
    TCATCTCTAC AATCATCAAA CTGTGTCCTG AGAGAGCTGG ACCTGAGTAA CAATGAGCTG
    CAGGATTCAG GAGTGAAGCT GATCTGTGCT GGATTGAAGA GCCCAAACTG TCAACTCAAC
    ACACTGAGAT TGTCAGGCTG TAATCTCACT GATCAGTGCT GTGAAACTGT GGCTTCATCT
    CTACAATTAT CCAACTGCCC TCTGAGAGAG CTGGACCTGA GTCACAATGA TCTTCAGGGT
    TCAAGAGTGA AGCTGATCTG TGCTGGACTG AAGAGCCCAA ACTGTCAACT CAACACACTG
    AGATTATCTT ACTGTTTGGT GTCAGAGGAA GGTTGTGCAG CTCTGGCTTC AGCTCTGAGC
    TCAAACCCGT CACACCTGAG AGAGCTGGAT CTGAGCTACA ATCACCCAGG AGAAGCAGGA
    CTCAAACTGC TCTCTGATCA GCTCAAACAC CTCCACAAAC TCAATGTGGA TCATGGAGGA
    GAATTCAGGA CTGTATCAAC ACTACACAAA TACGCCTGTG AACTCACACT GGATCCAAAC
    ACAGCACACA CACGCCTCGC TCTGTCTGAG GAGAACAGAA AGGCGACGCG TGTGAAGGAG
    AGTCAGTCTT ATCCTCGTCA TCCAGACAGA TTTGATAAGT TTCATCAGGT TATGAGTAAA
    GAGAGTCTGA CTGGACGCTG TTACTGGGAG ACTCAATGGA GCGGATATGC TGAAATATCA
    GTTTGTTATA AAGGAATCAA GAGGAAAGGA CGGAGTGACT GTCGGTTTGG ATGTAATGAT
    CAGTCCTGGA GACTTGATTG CTCTGATCAC TCATTCTCTG TCTGTCACAA TAATGAACTC
    ACACACATCT CAGCTCGTCC AGACTCCAGC AGGACAGTAG GAGTGTTTGT GGACGAGTCG
    AGCGGCTCTC TGTCCTTCTA CAGTGTCTCT GATAAACTCA CACACTTACA CACATTCAAC
    ACCACATTCA CCGACACACT CCACGCTGGA TTTAGACTTG GTTATAAATC TTCAGTGTCT
    CTGTGTCGCA TTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141857
    Clone ID Related Accession (Same CDS sequence) XM_021474773.1
    Accession Version XM_021474773.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGA AAGCTAAACA TCCATGTGAA ATGAGTGTGT TTGAAGAGAG AGAGAGGGAG 
    GCTTTCATTC AGACTGTGAG AGCAGTTTCT CCATTATCTA GCTGTGTGTC TATGATGTGT
    AACCACTCCA TTGGTCTCCC CCCTGATCGC AGTGATGGAG CTGTTAGCTC TGACTTTGTC
    TGGGAAAGGA CCAGATCTCC ATTATCTAGC TGTGTGTCTA TGAAGAGCAA CAACTCTATG
    GGTCTCCCCC CTGATCTCAG TGATGGAGCT GTCAACTCTG AGTTCCTCAA GAGAAAGAGA
    GAAGAGTCTT CATTGTCTAG CAGCATGTTT ATAAAGAGTG ACCACCCCTT TGGTCTCCCC
    CTTTATCTCA GTGATAGAGC TGTGAACTCT GACTCTGTCT GGGAAAGGAC CAGATCTCCA
    TTATCTAGCT GTGTGTCTAT GAAGAGCAAC AACTCTATGG GTCTCCCCCC TGATCTCAGT
    GATGGAGCTG TCAACTCTGA GTTCCTCAAG AGAAAGAGAG AAGAGTCTTC ATTGTCTAGC
    AGCATGTTTA TAAAGAGTGA CCACCCCTTT GGTCTCCCCC TTTATCTCAG TGATAGAGCT
    GTGAACTCTG ACTCTGTCTG GGAAAGGACC AGATCTCCAT TATCTAGCTG TGTGTCTATG
    AAGAGCAACA ACTCTATGGG TCTCCCCCCT GATCTCAGTG ATGGAGCTGT CAACTCTGAG
    TTCCTCTGGA AAAGGACCAG ATCTCCATTA TCTAGCTGTG TGTCTATGAA GAGCAACAAC
    TCTATGGGTC TCCCCCCTGA TCTCAGTGAT GGAGCTGTCA ACTCTGAGTT CCTCAAGAGA
    AAGAGAGAAG AGTCTTCATT GTCTAGCAGC ATGTCTATGA AGAGTGACCA CTACATTGAT
    CTCCCCCCAT GTAAACTCAT CAGCAATAGA AAGAGAGAGT CTTCTCCATT ATCTAGCTGT
    GTGTCTATGA AGAGTAATCA CTCCATGAGT CTCCCCCCTT ATCTCAGTGA TGGAGCTGTG
    AACTCTGACT CTGTATCTCC CTACTATCAG AAACACATCA GTCATTTTAA AACAGAGGCA
    TGCCTGATGA TTGATTCTCA ACAACTAGTG CATGATGATC TCCAGAGAGT CAAAGACCAG
    CACAGATTCA TCATGAAGAT CAAGTACGAG AGCTTATTTG AGGGAGTGAA ACTCCAGCAG
    AATCAAACCC TCCTGAACAG CATTTACACA CAGCTGTACA TCATAGAGGG AGAGAGTGAA
    GGAGTGAATG AAGAACATGA GGTGCTGCAG ATGGAGAAGA GATACAAGAC TCTCCAAGAC
    ACTCCAATCA ACTGCAATGA CATCTTTAAC CCTTCAGCTG AAGCAGGAGA TGAGGAGAAG
    AGCAGAGAGG AGAAAGAGCA GATCAAGACT GTTCTCACTA AAGGCATCGC TGGAATTGGA
    AAAACCGTCT CTGTGCAGAA GTTCATTCTG GACTGGGCCG AGGGAAAAGC CAATCAGGAT
    GTAGATTTCA TCTTTGTGCT TCCGTTTCGA GAGCTGAACT TGATTAAAGA TCATCAGTAC
    AGTCTTCACA CACTTCTGCT GGACTTTCAT CCTGAACTTC AGCATCTGGA CTCACAGATT
    TATGAGGAGT GTAAAGTCGT GTTCATCTTT GATGGTCTGG ATGAAAGCAG AATCACACTG
    ATGTTTTCAG ACTGTCAGAA AGTTTCTGAT GTGACTGAAG CTTCATCAGT GAGTGTGTTG
    ATGTCAAACC TCATGAAAGG AGATCTGCTT CCCTCCGCTC TCATCTGGAT CACCAGCAGA
    CCAGCAGCGG CCAATCAGAT CCCCTCAAAA CACATCAGCC TCGTGACGGA GATTCAGGGC
    TTCAATGACC CTCAGAAGGA GGAATATTTC AGGAAGAGAA TCAGAGATGA AGATCAAGCC
    AGCAGAATCA TCTCCCACAT CAGAAGAGCA AGAAGCCTCC ACATCATGTG CCACATACCC
    GTCTTCTGCT GGATCTCAGC CACTGTGCTT CAAACCATCC TGAGACAAGA TCTGAGTGCA
    GAAATCCCTC AAACTCTGAC TGAGATGTAC ATTCACTTCC TCCTCATTCA GACACACATG
    AGAAAGCAGA AGTATGAAGA GAGAGATCCA GAGAAACTCC TGCGGTTCAA CAGAGACGTG
    ATTGTGAAAC TTGCTGAACT GGCTTTCAAT CAGCTGATGA AGGGCAATGT GATGTTCTAC
    GAGGAGGACC TGATTGAGAG CAGGATAGAC GTCGCTGACG CCTCAGTGTA TTCTGGGATC
    TGCACTGAGA TCTTCAGAGA GGAATCTGTG ATTTATCAGA GGAAGGTTTA CAGCTTTGTT
    CATCTGAGCT TTCAGGAGTG TTTTGCAGCA CTGTATGTGT TTTACTGCTA TTTACACAAC
    AACACTGAGG TGATGAAGAT GTTCCTGACA GAAATATACA GAACTCAGAG TAAGAAAGTT
    TCTCTGGATG TGCTTCTGAA AGGAGTGATG ATTGAAGCCT TGAAAAGTAA AACTGGACAC
    CTGGATCTGT TCCTGCGATT TCTTCATGGC GTATCACTGG AGTCCAATCA GAGACTCTTA
    CAGGATCTGC TGAAACACAC AGAGAACGAC CCAGACAGCA TCAAGAAAAT AATCAAAAAC
    CTCAAACGAG GACAAAAAAA CAACGTCAGT CCTGAAAGAT GGATGAATCT CTCACACTGC
    TTGATGGAGA TGAAGGACAA TTCTGTGGTT AAAGAAATGC AAGCATTCTT AAAATCTAAA
    ACACAAAGAA AATGTCTTTC TCTTGCACAA TGTTCGACTT TGGCAAACAT GATCCTGATG
    TCAGAGGAGG TGCTGGATGA GCTAGACATT AAAAAGTACA ACATTAAATC AAAAGAAGGT
    CGATGGAGAC TGATACCCGC TGTGAGAAAC TGCAGAAAAG CTCTATTGTC AGAATGTAAT
    CTTGCAGATC AACACTGTGA AACTGTAGCT TCAGCTCTAC AATCATCAAA CAACCTCTTG
    AGAGAAGTGA ATCTGAGTCG CAATGAGCTG CAGGATTCAG GAGTGAAGCT GATCTGTGCT
    GGACTGAAGA GCCCAAACTG TCAACTCAAC GTTCTGAAAT TGTCAGGCTG TAATCTCACT
    AAACAGTCCT GTGAAACTGT GGCTGTGTCT CTACTTTCAT CAAACTGCCG CCTGAGAGAG
    CTGGACCTGA GTCGCAATGA CCTGCAGGAT TCAGGAGTGA AGCTGATCTG TGCTGGACTG
    AAAAGTGCAA ACTGTCAACT CAACACACTG AGACTGGCAA AATGTAACCT CACTGATCAG
    TGCTGCGAAA CTATATCTTC ATTTCTACAA TCATCAAACT CTGTCTTGAG AGAGCTGGAC
    ATAAGCAACA ATCGTCTGCA GGATTCAGGA GTGAAGCTGA TTTTTACTGC AATAAAGAGT
    GAGAACTGTC AACTCAACAT CCTGAGATTG TCAAGTTGTA ACCTCACTGA TCAGTGCTGT
    GAAACTTTGG CTTCATCTCT ACAATCATCA AACTGTGTCC TGAGAGAGCT GGACCTGAGT
    AACAATGAGC TGCAGGATTC AGGAGTGAAG CTGATCTGTG CTGGATTGAA GAGCCCAAAC
    TGTCAACTCA ACACACTGAG ATTGTCAGGC TGTAATCTCA CTGATCAGTG CTGTGAAACT
    GTGGCTTCAT CTCTACAATT ATCCAACTGC CCTCTGAGAG AGCTGGACCT GAGTCACAAT
    GATCTTCAGG GTTCAAGAGT GAAGCTGATC TGTGCTGGAC TGAAGAGCCC AAACTGTCAA
    CTCAACACAC TGAGATTATC TTACTGTTTG GTGTCAGAGG AAGGTTGTGC AGCTCTGGCT
    TCAGCTCTGA GCTCAAACCC GTCACACCTG AGAGAGCTGG ATCTGAGCTA CAATCACCCA
    GGAGAAGCAG GACTCAAACT GCTCTCTGAT CAGCTCAAAC ACCTCCACAA ACTCAATGTG
    GATCATGGAG GAGAATTCAG GACTGTATCA ACACTACACA AATACGCCTG TGAACTCACA
    CTGGATCCAA ACACAGCACA CACACGCCTC GCTCTGTCTG AGGAGAACAG AAAGGCGACG
    CGTGTGAAGG AGAGTCAGTC TTATCCTCGT CATCCAGACA GATTTGATAA GTTTCATCAG
    GTTATGAGTA AAGAGAGTCT GACTGGACGC TGTTACTGGG AGACTCAATG GAGCGGATAT
    GCTGAAATAT CAGTTTGTTA TAAAGGAATC AAGAGGAAAG GACGGAGTGA CTGTCGGTTT
    GGATGTAATG ATCAGTCCTG GAGACTTGAT TGCTCTGATC ACTCATTCTC TGTCTGTCAC
    AATAATGAAC TCACACACAT CTCAGCTCGT CCAGACTCCA GCAGGACAGT AGGAGTGTTT
    GTGGACGAGT CGAGCGGCTC TCTGTCCTTC TACAGTGTCT CTGATAAACT CACACACTTA
    CACACATTCA ACACCACATT CACCGACACA CTCCACGCTG GATTTAGACT TGGTTATAAA
    TCTTCAGTGT CTCTGTGTCG CATTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330448.1
    CDS160..4689
    Translation

    Target ORF information:

    RefSeq Version XM_021474773.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-214b16.3 (si:ch211-214b16.3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474773.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGA AAGCTAAACA TCCATGTGAA ATGAGTGTGT TTGAAGAGAG AGAGAGGGAG 
    GCTTTCATTC AGACTGTGAG AGCAGTTTCT CCATTATCTA GCTGTGTGTC TATGATGTGT
    AACCACTCCA TTGGTCTCCC CCCTGATCGC AGTGATGGAG CTGTTAGCTC TGACTTTGTC
    TGGGAAAGGA CCAGATCTCC ATTATCTAGC TGTGTGTCTA TGAAGAGCAA CAACTCTATG
    GGTCTCCCCC CTGATCTCAG TGATGGAGCT GTCAACTCTG AGTTCCTCAA GAGAAAGAGA
    GAAGAGTCTT CATTGTCTAG CAGCATGTTT ATAAAGAGTG ACCACCCCTT TGGTCTCCCC
    CTTTATCTCA GTGATAGAGC TGTGAACTCT GACTCTGTCT GGGAAAGGAC CAGATCTCCA
    TTATCTAGCT GTGTGTCTAT GAAGAGCAAC AACTCTATGG GTCTCCCCCC TGATCTCAGT
    GATGGAGCTG TCAACTCTGA GTTCCTCAAG AGAAAGAGAG AAGAGTCTTC ATTGTCTAGC
    AGCATGTTTA TAAAGAGTGA CCACCCCTTT GGTCTCCCCC TTTATCTCAG TGATAGAGCT
    GTGAACTCTG ACTCTGTCTG GGAAAGGACC AGATCTCCAT TATCTAGCTG TGTGTCTATG
    AAGAGCAACA ACTCTATGGG TCTCCCCCCT GATCTCAGTG ATGGAGCTGT CAACTCTGAG
    TTCCTCTGGA AAAGGACCAG ATCTCCATTA TCTAGCTGTG TGTCTATGAA GAGCAACAAC
    TCTATGGGTC TCCCCCCTGA TCTCAGTGAT GGAGCTGTCA ACTCTGAGTT CCTCAAGAGA
    AAGAGAGAAG AGTCTTCATT GTCTAGCAGC ATGTCTATGA AGAGTGACCA CTACATTGAT
    CTCCCCCCAT GTAAACTCAT CAGCAATAGA AAGAGAGAGT CTTCTCCATT ATCTAGCTGT
    GTGTCTATGA AGAGTAATCA CTCCATGAGT CTCCCCCCTT ATCTCAGTGA TGGAGCTGTG
    AACTCTGACT CTGTATCTCC CTACTATCAG AAACACATCA GTCATTTTAA AACAGAGGCA
    TGCCTGATGA TTGATTCTCA ACAACTAGTG CATGATGATC TCCAGAGAGT CAAAGACCAG
    CACAGATTCA TCATGAAGAT CAAGTACGAG AGCTTATTTG AGGGAGTGAA ACTCCAGCAG
    AATCAAACCC TCCTGAACAG CATTTACACA CAGCTGTACA TCATAGAGGG AGAGAGTGAA
    GGAGTGAATG AAGAACATGA GGTGCTGCAG ATGGAGAAGA GATACAAGAC TCTCCAAGAC
    ACTCCAATCA ACTGCAATGA CATCTTTAAC CCTTCAGCTG AAGCAGGAGA TGAGGAGAAG
    AGCAGAGAGG AGAAAGAGCA GATCAAGACT GTTCTCACTA AAGGCATCGC TGGAATTGGA
    AAAACCGTCT CTGTGCAGAA GTTCATTCTG GACTGGGCCG AGGGAAAAGC CAATCAGGAT
    GTAGATTTCA TCTTTGTGCT TCCGTTTCGA GAGCTGAACT TGATTAAAGA TCATCAGTAC
    AGTCTTCACA CACTTCTGCT GGACTTTCAT CCTGAACTTC AGCATCTGGA CTCACAGATT
    TATGAGGAGT GTAAAGTCGT GTTCATCTTT GATGGTCTGG ATGAAAGCAG AATCACACTG
    ATGTTTTCAG ACTGTCAGAA AGTTTCTGAT GTGACTGAAG CTTCATCAGT GAGTGTGTTG
    ATGTCAAACC TCATGAAAGG AGATCTGCTT CCCTCCGCTC TCATCTGGAT CACCAGCAGA
    CCAGCAGCGG CCAATCAGAT CCCCTCAAAA CACATCAGCC TCGTGACGGA GATTCAGGGC
    TTCAATGACC CTCAGAAGGA GGAATATTTC AGGAAGAGAA TCAGAGATGA AGATCAAGCC
    AGCAGAATCA TCTCCCACAT CAGAAGAGCA AGAAGCCTCC ACATCATGTG CCACATACCC
    GTCTTCTGCT GGATCTCAGC CACTGTGCTT CAAACCATCC TGAGACAAGA TCTGAGTGCA
    GAAATCCCTC AAACTCTGAC TGAGATGTAC ATTCACTTCC TCCTCATTCA GACACACATG
    AGAAAGCAGA AGTATGAAGA GAGAGATCCA GAGAAACTCC TGCGGTTCAA CAGAGACGTG
    ATTGTGAAAC TTGCTGAACT GGCTTTCAAT CAGCTGATGA AGGGCAATGT GATGTTCTAC
    GAGGAGGACC TGATTGAGAG CAGGATAGAC GTCGCTGACG CCTCAGTGTA TTCTGGGATC
    TGCACTGAGA TCTTCAGAGA GGAATCTGTG ATTTATCAGA GGAAGGTTTA CAGCTTTGTT
    CATCTGAGCT TTCAGGAGTG TTTTGCAGCA CTGTATGTGT TTTACTGCTA TTTACACAAC
    AACACTGAGG TGATGAAGAT GTTCCTGACA GAAATATACA GAACTCAGAG TAAGAAAGTT
    TCTCTGGATG TGCTTCTGAA AGGAGTGATG ATTGAAGCCT TGAAAAGTAA AACTGGACAC
    CTGGATCTGT TCCTGCGATT TCTTCATGGC GTATCACTGG AGTCCAATCA GAGACTCTTA
    CAGGATCTGC TGAAACACAC AGAGAACGAC CCAGACAGCA TCAAGAAAAT AATCAAAAAC
    CTCAAACGAG GACAAAAAAA CAACGTCAGT CCTGAAAGAT GGATGAATCT CTCACACTGC
    TTGATGGAGA TGAAGGACAA TTCTGTGGTT AAAGAAATGC AAGCATTCTT AAAATCTAAA
    ACACAAAGAA AATGTCTTTC TCTTGCACAA TGTTCGACTT TGGCAAACAT GATCCTGATG
    TCAGAGGAGG TGCTGGATGA GCTAGACATT AAAAAGTACA ACATTAAATC AAAAGAAGGT
    CGATGGAGAC TGATACCCGC TGTGAGAAAC TGCAGAAAAG CTCTATTGTC AGAATGTAAT
    CTTGCAGATC AACACTGTGA AACTGTAGCT TCAGCTCTAC AATCATCAAA CAACCTCTTG
    AGAGAAGTGA ATCTGAGTCG CAATGAGCTG CAGGATTCAG GAGTGAAGCT GATCTGTGCT
    GGACTGAAGA GCCCAAACTG TCAACTCAAC GTTCTGAAAT TGTCAGGCTG TAATCTCACT
    AAACAGTCCT GTGAAACTGT GGCTGTGTCT CTACTTTCAT CAAACTGCCG CCTGAGAGAG
    CTGGACCTGA GTCGCAATGA CCTGCAGGAT TCAGGAGTGA AGCTGATCTG TGCTGGACTG
    AAAAGTGCAA ACTGTCAACT CAACACACTG AGACTGGCAA AATGTAACCT CACTGATCAG
    TGCTGCGAAA CTATATCTTC ATTTCTACAA TCATCAAACT CTGTCTTGAG AGAGCTGGAC
    ATAAGCAACA ATCGTCTGCA GGATTCAGGA GTGAAGCTGA TTTTTACTGC AATAAAGAGT
    GAGAACTGTC AACTCAACAT CCTGAGATTG TCAAGTTGTA ACCTCACTGA TCAGTGCTGT
    GAAACTTTGG CTTCATCTCT ACAATCATCA AACTGTGTCC TGAGAGAGCT GGACCTGAGT
    AACAATGAGC TGCAGGATTC AGGAGTGAAG CTGATCTGTG CTGGATTGAA GAGCCCAAAC
    TGTCAACTCA ACACACTGAG ATTGTCAGGC TGTAATCTCA CTGATCAGTG CTGTGAAACT
    GTGGCTTCAT CTCTACAATT ATCCAACTGC CCTCTGAGAG AGCTGGACCT GAGTCACAAT
    GATCTTCAGG GTTCAAGAGT GAAGCTGATC TGTGCTGGAC TGAAGAGCCC AAACTGTCAA
    CTCAACACAC TGAGATTATC TTACTGTTTG GTGTCAGAGG AAGGTTGTGC AGCTCTGGCT
    TCAGCTCTGA GCTCAAACCC GTCACACCTG AGAGAGCTGG ATCTGAGCTA CAATCACCCA
    GGAGAAGCAG GACTCAAACT GCTCTCTGAT CAGCTCAAAC ACCTCCACAA ACTCAATGTG
    GATCATGGAG GAGAATTCAG GACTGTATCA ACACTACACA AATACGCCTG TGAACTCACA
    CTGGATCCAA ACACAGCACA CACACGCCTC GCTCTGTCTG AGGAGAACAG AAAGGCGACG
    CGTGTGAAGG AGAGTCAGTC TTATCCTCGT CATCCAGACA GATTTGATAA GTTTCATCAG
    GTTATGAGTA AAGAGAGTCT GACTGGACGC TGTTACTGGG AGACTCAATG GAGCGGATAT
    GCTGAAATAT CAGTTTGTTA TAAAGGAATC AAGAGGAAAG GACGGAGTGA CTGTCGGTTT
    GGATGTAATG ATCAGTCCTG GAGACTTGAT TGCTCTGATC ACTCATTCTC TGTCTGTCAC
    AATAATGAAC TCACACACAT CTCAGCTCGT CCAGACTCCA GCAGGACAGT AGGAGTGTTT
    GTGGACGAGT CGAGCGGCTC TCTGTCCTTC TACAGTGTCT CTGATAAACT CACACACTTA
    CACACATTCA ACACCACATT CACCGACACA CTCCACGCTG GATTTAGACT TGGTTATAAA
    TCTTCAGTGT CTCTGTGTCG CATTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141858
    Clone ID Related Accession (Same CDS sequence) XM_021474774.1
    Accession Version XM_021474774.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGA AAGCTAAACA TCCATGTGAA ATGAGTGTGT TTGAAGAGAG AGAGAGGGAG 
    GCTTTCATTC AGACTGTGAG AGCAGTTTCT CCATTATCTA GCTGTGTGTC TATGATGTGT
    AACCACTCCA TTGGTCTCCC CCCTGATCGC AGTGATGGAG CTGTTAGCTC TGACTTTGTC
    TGGGAAAGGA CCAGATCTCC ATTATCTAGC TGTGTGTCTA TGAAGAGCAA CAACTCTATG
    GGTCTCCCCC CTGATCTCAG TGATGGAGCT GTCAACTCTG AGTTCCTCTG GGAAAGGACC
    AGATCTCCAT TATCTAGCTG TGTGTCTATG AAGAGCAACA ACTCTATGGG TCTCCCCCCT
    GATCTCAGTG ATGGAGCTGT CAACTCTGAG TTCCTCAAGA GAAAGAGAGA AGAGTCTTCA
    TTGTCTAGCA GCATGTTTAT AAAGAGTGAC CACCCCTTTG GTCTCCCCCT TTATCTCAGT
    GATAGAGCTG TGAACTCTGA CTCTGTCTGG GAAAGGACCA GATCTCCATT ATCTAGCTGT
    GTGTCTATGA AGAGCAACAA CTCTATGGGT CTCCCCCCTG ATCTCAGTGA TGGAGCTGTC
    AACTCTGAGT TCCTCAAGAG AAAGAGAGAA GAGTCTTCAT TGTCTAGCAG CATGTTTATA
    AAGAGTGACC ACCCCTTTGG TCTCCCCCTT TATCTCAGTG ATAGAGCTGT GAACTCTGAC
    TCTGTCTGGA AAAGGACCAG ATCTCCATTA TCTAGCTGTG TGTCTATGAA GAGCAACAAC
    TCTATGGGTC TCCCCCCTGA TCTCAGTGAT GGAGCTGTCA ACTCTGAGTT CCTCAAGAGA
    AAGAGAGAAG AGTCTTCATT GTCTAGCAGC ATGTCTATGA AGAGTGACCA CTACATTGAT
    CTCCCCCCAT GTAAACTCAT CAGCAATAGA AAGAGAGAGT CTTCTCCATT ATCTAGCTGT
    GTGTCTATGA AGAGTAATCA CTCCATGAGT CTCCCCCCTT ATCTCAGTGA TGGAGCTGTG
    AACTCTGACT CTGTATCTCC CTACTATCAG AAACACATCA GTCATTTTAA AACAGAGGCA
    TGCCTGATGA TTGATTCTCA ACAACTAGTG CATGATGATC TCCAGAGAGT CAAAGACCAG
    CACAGATTCA TCATGAAGAT CAAGTACGAG AGCTTATTTG AGGGAGTGAA ACTCCAGCAG
    AATCAAACCC TCCTGAACAG CATTTACACA CAGCTGTACA TCATAGAGGG AGAGAGTGAA
    GGAGTGAATG AAGAACATGA GGTGCTGCAG ATGGAGAAGA GATACAAGAC TCTCCAAGAC
    ACTCCAATCA ACTGCAATGA CATCTTTAAC CCTTCAGCTG AAGCAGGAGA TGAGGAGAAG
    AGCAGAGAGG AGAAAGAGCA GATCAAGACT GTTCTCACTA AAGGCATCGC TGGAATTGGA
    AAAACCGTCT CTGTGCAGAA GTTCATTCTG GACTGGGCCG AGGGAAAAGC CAATCAGGAT
    GTAGATTTCA TCTTTGTGCT TCCGTTTCGA GAGCTGAACT TGATTAAAGA TCATCAGTAC
    AGTCTTCACA CACTTCTGCT GGACTTTCAT CCTGAACTTC AGCATCTGGA CTCACAGATT
    TATGAGGAGT GTAAAGTCGT GTTCATCTTT GATGGTCTGG ATGAAAGCAG AATCACACTG
    ATGTTTTCAG ACTGTCAGAA AGTTTCTGAT GTGACTGAAG CTTCATCAGT GAGTGTGTTG
    ATGTCAAACC TCATGAAAGG AGATCTGCTT CCCTCCGCTC TCATCTGGAT CACCAGCAGA
    CCAGCAGCGG CCAATCAGAT CCCCTCAAAA CACATCAGCC TCGTGACGGA GATTCAGGGC
    TTCAATGACC CTCAGAAGGA GGAATATTTC AGGAAGAGAA TCAGAGATGA AGATCAAGCC
    AGCAGAATCA TCTCCCACAT CAGAAGAGCA AGAAGCCTCC ACATCATGTG CCACATACCC
    GTCTTCTGCT GGATCTCAGC CACTGTGCTT CAAACCATCC TGAGACAAGA TCTGAGTGCA
    GAAATCCCTC AAACTCTGAC TGAGATGTAC ATTCACTTCC TCCTCATTCA GACACACATG
    AGAAAGCAGA AGTATGAAGA GAGAGATCCA GAGAAACTCC TGCGGTTCAA CAGAGACGTG
    ATTGTGAAAC TTGCTGAACT GGCTTTCAAT CAGCTGATGA AGGGCAATGT GATGTTCTAC
    GAGGAGGACC TGATTGAGAG CAGGATAGAC GTCGCTGACG CCTCAGTGTA TTCTGGGATC
    TGCACTGAGA TCTTCAGAGA GGAATCTGTG ATTTATCAGA GGAAGGTTTA CAGCTTTGTT
    CATCTGAGCT TTCAGGAGTG TTTTGCAGCA CTGTATGTGT TTTACTGCTA TTTACACAAC
    AACACTGAGG TGATGAAGAT GTTCCTGACA GAAATATACA GAACTCAGAG TAAGAAAGTT
    TCTCTGGATG TGCTTCTGAA AGGAGTGATG ATTGAAGCCT TGAAAAGTAA AACTGGACAC
    CTGGATCTGT TCCTGCGATT TCTTCATGGC GTATCACTGG AGTCCAATCA GAGACTCTTA
    CAGGATCTGC TGAAACACAC AGAGAACGAC CCAGACAGCA TCAAGAAAAT AATCAAAAAC
    CTCAAACGAG GACAAAAAAA CAACGTCAGT CCTGAAAGAT GGATGAATCT CTCACACTGC
    TTGATGGAGA TGAAGGACAA TTCTGTGGTT AAAGAAATGC AAGCATTCTT AAAATCTAAA
    ACACAAAGAA AATGTCTTTC TCTTGCACAA TGTTCGACTT TGGCAAACAT GATCCTGATG
    TCAGAGGAGG TGCTGGATGA GCTAGACATT AAAAAGTACA ACATTAAATC AAAAGAAGGT
    CGATGGAGAC TGATACCCGC TGTGAGAAAC TGCAGAAAAG CTCTATTGTC AGAATGTAAT
    CTTGCAGATC AACACTGTGA AACTGTAGCT TCAGCTCTAC AATCATCAAA CAACCTCTTG
    AGAGAAGTGA ATCTGAGTCG CAATGAGCTG CAGGATTCAG GAGTGAAGCT GATCTGTGCT
    GGACTGAAGA GCCCAAACTG TCAACTCAAC GTTCTGAAAT TGTCAGGCTG TAATCTCACT
    AAACAGTCCT GTGAAACTGT GGCTGTGTCT CTACTTTCAT CAAACTGCCG CCTGAGAGAG
    CTGGACCTGA GTCGCAATGA CCTGCAGGAT TCAGGAGTGA AGCTGATCTG TGCTGGACTG
    AAAAGTGCAA ACTGTCAACT CAACACACTG AGACTGGCAA AATGTAACCT CACTGATCAG
    TGCTGCGAAA CTATATCTTC ATTTCTACAA TCATCAAACT CTGTCTTGAG AGAGCTGGAC
    ATAAGCAACA ATCGTCTGCA GGATTCAGGA GTGAAGCTGA TTTTTACTGC AATAAAGAGT
    GAGAACTGTC AACTCAACAT CCTGAGATTG TCAAGTTGTA ACCTCACTGA TCAGTGCTGT
    GAAACTTTGG CTTCATCTCT ACAATCATCA AACTGTGTCC TGAGAGAGCT GGACCTGAGT
    AACAATGAGC TGCAGGATTC AGGAGTGAAG CTGATCTGTG CTGGATTGAA GAGCCCAAAC
    TGTCAACTCA ACACACTGAG ATTGTCAGGC TGTAATCTCA CTGATCAGTG CTGTGAAACT
    GTGGCTTCAT CTCTACAATT ATCCAACTGC CCTCTGAGAG AGCTGGACCT GAGTCACAAT
    GATCTTCAGG GTTCAAGAGT GAAGCTGATC TGTGCTGGAC TGAAGAGCCC AAACTGTCAA
    CTCAACACAC TGAGATTATC TTACTGTTTG GTGTCAGAGG AAGGTTGTGC AGCTCTGGCT
    TCAGCTCTGA GCTCAAACCC GTCACACCTG AGAGAGCTGG ATCTGAGCTA CAATCACCCA
    GGAGAAGCAG GACTCAAACT GCTCTCTGAT CAGCTCAAAC ACCTCCACAA ACTCAATGTG
    GATCATGGAG GAGAATTCAG GACTGTATCA ACACTACACA AATACGCCTG TGAACTCACA
    CTGGATCCAA ACACAGCACA CACACGCCTC GCTCTGTCTG AGGAGAACAG AAAGGCGACG
    CGTGTGAAGG AGAGTCAGTC TTATCCTCGT CATCCAGACA GATTTGATAA GTTTCATCAG
    GTTATGAGTA AAGAGAGTCT GACTGGACGC TGTTACTGGG AGACTCAATG GAGCGGATAT
    GCTGAAATAT CAGTTTGTTA TAAAGGAATC AAGAGGAAAG GACGGAGTGA CTGTCGGTTT
    GGATGTAATG ATCAGTCCTG GAGACTTGAT TGCTCTGATC ACTCATTCTC TGTCTGTCAC
    AATAATGAAC TCACACACAT CTCAGCTCGT CCAGACTCCA GCAGGACAGT AGGAGTGTTT
    GTGGACGAGT CGAGCGGCTC TCTGTCCTTC TACAGTGTCT CTGATAAACT CACACACTTA
    CACACATTCA ACACCACATT CACCGACACA CTCCACGCTG GATTTAGACT TGGTTATAAA
    TCTTCAGTGT CTCTGTGTCG CATTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330449.1
    CDS160..4689
    Translation

    Target ORF information:

    RefSeq Version XM_021474774.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-214b16.3 (si:ch211-214b16.3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474774.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGA AAGCTAAACA TCCATGTGAA ATGAGTGTGT TTGAAGAGAG AGAGAGGGAG 
    GCTTTCATTC AGACTGTGAG AGCAGTTTCT CCATTATCTA GCTGTGTGTC TATGATGTGT
    AACCACTCCA TTGGTCTCCC CCCTGATCGC AGTGATGGAG CTGTTAGCTC TGACTTTGTC
    TGGGAAAGGA CCAGATCTCC ATTATCTAGC TGTGTGTCTA TGAAGAGCAA CAACTCTATG
    GGTCTCCCCC CTGATCTCAG TGATGGAGCT GTCAACTCTG AGTTCCTCTG GGAAAGGACC
    AGATCTCCAT TATCTAGCTG TGTGTCTATG AAGAGCAACA ACTCTATGGG TCTCCCCCCT
    GATCTCAGTG ATGGAGCTGT CAACTCTGAG TTCCTCAAGA GAAAGAGAGA AGAGTCTTCA
    TTGTCTAGCA GCATGTTTAT AAAGAGTGAC CACCCCTTTG GTCTCCCCCT TTATCTCAGT
    GATAGAGCTG TGAACTCTGA CTCTGTCTGG GAAAGGACCA GATCTCCATT ATCTAGCTGT
    GTGTCTATGA AGAGCAACAA CTCTATGGGT CTCCCCCCTG ATCTCAGTGA TGGAGCTGTC
    AACTCTGAGT TCCTCAAGAG AAAGAGAGAA GAGTCTTCAT TGTCTAGCAG CATGTTTATA
    AAGAGTGACC ACCCCTTTGG TCTCCCCCTT TATCTCAGTG ATAGAGCTGT GAACTCTGAC
    TCTGTCTGGA AAAGGACCAG ATCTCCATTA TCTAGCTGTG TGTCTATGAA GAGCAACAAC
    TCTATGGGTC TCCCCCCTGA TCTCAGTGAT GGAGCTGTCA ACTCTGAGTT CCTCAAGAGA
    AAGAGAGAAG AGTCTTCATT GTCTAGCAGC ATGTCTATGA AGAGTGACCA CTACATTGAT
    CTCCCCCCAT GTAAACTCAT CAGCAATAGA AAGAGAGAGT CTTCTCCATT ATCTAGCTGT
    GTGTCTATGA AGAGTAATCA CTCCATGAGT CTCCCCCCTT ATCTCAGTGA TGGAGCTGTG
    AACTCTGACT CTGTATCTCC CTACTATCAG AAACACATCA GTCATTTTAA AACAGAGGCA
    TGCCTGATGA TTGATTCTCA ACAACTAGTG CATGATGATC TCCAGAGAGT CAAAGACCAG
    CACAGATTCA TCATGAAGAT CAAGTACGAG AGCTTATTTG AGGGAGTGAA ACTCCAGCAG
    AATCAAACCC TCCTGAACAG CATTTACACA CAGCTGTACA TCATAGAGGG AGAGAGTGAA
    GGAGTGAATG AAGAACATGA GGTGCTGCAG ATGGAGAAGA GATACAAGAC TCTCCAAGAC
    ACTCCAATCA ACTGCAATGA CATCTTTAAC CCTTCAGCTG AAGCAGGAGA TGAGGAGAAG
    AGCAGAGAGG AGAAAGAGCA GATCAAGACT GTTCTCACTA AAGGCATCGC TGGAATTGGA
    AAAACCGTCT CTGTGCAGAA GTTCATTCTG GACTGGGCCG AGGGAAAAGC CAATCAGGAT
    GTAGATTTCA TCTTTGTGCT TCCGTTTCGA GAGCTGAACT TGATTAAAGA TCATCAGTAC
    AGTCTTCACA CACTTCTGCT GGACTTTCAT CCTGAACTTC AGCATCTGGA CTCACAGATT
    TATGAGGAGT GTAAAGTCGT GTTCATCTTT GATGGTCTGG ATGAAAGCAG AATCACACTG
    ATGTTTTCAG ACTGTCAGAA AGTTTCTGAT GTGACTGAAG CTTCATCAGT GAGTGTGTTG
    ATGTCAAACC TCATGAAAGG AGATCTGCTT CCCTCCGCTC TCATCTGGAT CACCAGCAGA
    CCAGCAGCGG CCAATCAGAT CCCCTCAAAA CACATCAGCC TCGTGACGGA GATTCAGGGC
    TTCAATGACC CTCAGAAGGA GGAATATTTC AGGAAGAGAA TCAGAGATGA AGATCAAGCC
    AGCAGAATCA TCTCCCACAT CAGAAGAGCA AGAAGCCTCC ACATCATGTG CCACATACCC
    GTCTTCTGCT GGATCTCAGC CACTGTGCTT CAAACCATCC TGAGACAAGA TCTGAGTGCA
    GAAATCCCTC AAACTCTGAC TGAGATGTAC ATTCACTTCC TCCTCATTCA GACACACATG
    AGAAAGCAGA AGTATGAAGA GAGAGATCCA GAGAAACTCC TGCGGTTCAA CAGAGACGTG
    ATTGTGAAAC TTGCTGAACT GGCTTTCAAT CAGCTGATGA AGGGCAATGT GATGTTCTAC
    GAGGAGGACC TGATTGAGAG CAGGATAGAC GTCGCTGACG CCTCAGTGTA TTCTGGGATC
    TGCACTGAGA TCTTCAGAGA GGAATCTGTG ATTTATCAGA GGAAGGTTTA CAGCTTTGTT
    CATCTGAGCT TTCAGGAGTG TTTTGCAGCA CTGTATGTGT TTTACTGCTA TTTACACAAC
    AACACTGAGG TGATGAAGAT GTTCCTGACA GAAATATACA GAACTCAGAG TAAGAAAGTT
    TCTCTGGATG TGCTTCTGAA AGGAGTGATG ATTGAAGCCT TGAAAAGTAA AACTGGACAC
    CTGGATCTGT TCCTGCGATT TCTTCATGGC GTATCACTGG AGTCCAATCA GAGACTCTTA
    CAGGATCTGC TGAAACACAC AGAGAACGAC CCAGACAGCA TCAAGAAAAT AATCAAAAAC
    CTCAAACGAG GACAAAAAAA CAACGTCAGT CCTGAAAGAT GGATGAATCT CTCACACTGC
    TTGATGGAGA TGAAGGACAA TTCTGTGGTT AAAGAAATGC AAGCATTCTT AAAATCTAAA
    ACACAAAGAA AATGTCTTTC TCTTGCACAA TGTTCGACTT TGGCAAACAT GATCCTGATG
    TCAGAGGAGG TGCTGGATGA GCTAGACATT AAAAAGTACA ACATTAAATC AAAAGAAGGT
    CGATGGAGAC TGATACCCGC TGTGAGAAAC TGCAGAAAAG CTCTATTGTC AGAATGTAAT
    CTTGCAGATC AACACTGTGA AACTGTAGCT TCAGCTCTAC AATCATCAAA CAACCTCTTG
    AGAGAAGTGA ATCTGAGTCG CAATGAGCTG CAGGATTCAG GAGTGAAGCT GATCTGTGCT
    GGACTGAAGA GCCCAAACTG TCAACTCAAC GTTCTGAAAT TGTCAGGCTG TAATCTCACT
    AAACAGTCCT GTGAAACTGT GGCTGTGTCT CTACTTTCAT CAAACTGCCG CCTGAGAGAG
    CTGGACCTGA GTCGCAATGA CCTGCAGGAT TCAGGAGTGA AGCTGATCTG TGCTGGACTG
    AAAAGTGCAA ACTGTCAACT CAACACACTG AGACTGGCAA AATGTAACCT CACTGATCAG
    TGCTGCGAAA CTATATCTTC ATTTCTACAA TCATCAAACT CTGTCTTGAG AGAGCTGGAC
    ATAAGCAACA ATCGTCTGCA GGATTCAGGA GTGAAGCTGA TTTTTACTGC AATAAAGAGT
    GAGAACTGTC AACTCAACAT CCTGAGATTG TCAAGTTGTA ACCTCACTGA TCAGTGCTGT
    GAAACTTTGG CTTCATCTCT ACAATCATCA AACTGTGTCC TGAGAGAGCT GGACCTGAGT
    AACAATGAGC TGCAGGATTC AGGAGTGAAG CTGATCTGTG CTGGATTGAA GAGCCCAAAC
    TGTCAACTCA ACACACTGAG ATTGTCAGGC TGTAATCTCA CTGATCAGTG CTGTGAAACT
    GTGGCTTCAT CTCTACAATT ATCCAACTGC CCTCTGAGAG AGCTGGACCT GAGTCACAAT
    GATCTTCAGG GTTCAAGAGT GAAGCTGATC TGTGCTGGAC TGAAGAGCCC AAACTGTCAA
    CTCAACACAC TGAGATTATC TTACTGTTTG GTGTCAGAGG AAGGTTGTGC AGCTCTGGCT
    TCAGCTCTGA GCTCAAACCC GTCACACCTG AGAGAGCTGG ATCTGAGCTA CAATCACCCA
    GGAGAAGCAG GACTCAAACT GCTCTCTGAT CAGCTCAAAC ACCTCCACAA ACTCAATGTG
    GATCATGGAG GAGAATTCAG GACTGTATCA ACACTACACA AATACGCCTG TGAACTCACA
    CTGGATCCAA ACACAGCACA CACACGCCTC GCTCTGTCTG AGGAGAACAG AAAGGCGACG
    CGTGTGAAGG AGAGTCAGTC TTATCCTCGT CATCCAGACA GATTTGATAA GTTTCATCAG
    GTTATGAGTA AAGAGAGTCT GACTGGACGC TGTTACTGGG AGACTCAATG GAGCGGATAT
    GCTGAAATAT CAGTTTGTTA TAAAGGAATC AAGAGGAAAG GACGGAGTGA CTGTCGGTTT
    GGATGTAATG ATCAGTCCTG GAGACTTGAT TGCTCTGATC ACTCATTCTC TGTCTGTCAC
    AATAATGAAC TCACACACAT CTCAGCTCGT CCAGACTCCA GCAGGACAGT AGGAGTGTTT
    GTGGACGAGT CGAGCGGCTC TCTGTCCTTC TACAGTGTCT CTGATAAACT CACACACTTA
    CACACATTCA ACACCACATT CACCGACACA CTCCACGCTG GATTTAGACT TGGTTATAAA
    TCTTCAGTGT CTCTGTGTCG CATTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa141859
    Clone ID Related Accession (Same CDS sequence) XM_021474775.1
    Accession Version XM_021474775.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4530bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGA AAGCTAAACA TCCATGTGAA ATGAGTGTGT TTGAAGAGAG AGAGAGGGAG 
    GCTTTCATTC AGACTGTGAG AGCAGTTTCT CCATTATCTA GCTGTGTGTC TATGATGTGT
    AACCACTCCA TTGGTCTCCC CCCTGATCGC AGTGATGGAG CTGTTAGCTC TGACTTTGTC
    TGGGAAAGGA CCAGATCTCC ATTATCTAGC TGTGTGTCTA TGAAGAGCAA CAACTCTATG
    GGTCTCCCCC CTGATCTCAG TGATGGAGCT GTCAACTCTG AGTTCCTCAA GAGAAAGAGA
    GAAGAGTCTT CATTGTCTAG CAGCATGTTT ATAAAGAGTG ACCACCCCTT TGGTCTCCCC
    CTTTATCTCA GTGATAGAGC TGTGAACTCT GACTCTGTCT GGGAAAGGAC CAGATCTCCA
    TTATCTAGCT GTGTGTCTAT GAAGAGCAAC AACTCTATGG GTCTCCCCCC TGATCTCAGT
    GATGGAGCTG TCAACTCTGA GTTCCTCTGG GAAAGGACCA GATCTCCATT ATCTAGCTGT
    GTGTCTATGA AGAGCAACAA CTCTATGGGT CTCCCCCCTG ATCTCAGTGA TGGAGCTGTC
    AACTCTGAGT TCCTCAAGAG AAAGAGAGAA GAGTCTTCAT TGTCTAGCAG CATGTTTATA
    AAGAGTGACC ACCCCTTTGG TCTCCCCCTT TATCTCAGTG ATAGAGCTGT GAACTCTGAC
    TCTGTCTGGA AAAGGACCAG ATCTCCATTA TCTAGCTGTG TGTCTATGAA GAGCAACAAC
    TCTATGGGTC TCCCCCCTGA TCTCAGTGAT GGAGCTGTCA ACTCTGAGTT CCTCAAGAGA
    AAGAGAGAAG AGTCTTCATT GTCTAGCAGC ATGTCTATGA AGAGTGACCA CTACATTGAT
    CTCCCCCCAT GTAAACTCAT CAGCAATAGA AAGAGAGAGT CTTCTCCATT ATCTAGCTGT
    GTGTCTATGA AGAGTAATCA CTCCATGAGT CTCCCCCCTT ATCTCAGTGA TGGAGCTGTG
    AACTCTGACT CTGTATCTCC CTACTATCAG AAACACATCA GTCATTTTAA AACAGAGGCA
    TGCCTGATGA TTGATTCTCA ACAACTAGTG CATGATGATC TCCAGAGAGT CAAAGACCAG
    CACAGATTCA TCATGAAGAT CAAGTACGAG AGCTTATTTG AGGGAGTGAA ACTCCAGCAG
    AATCAAACCC TCCTGAACAG CATTTACACA CAGCTGTACA TCATAGAGGG AGAGAGTGAA
    GGAGTGAATG AAGAACATGA GGTGCTGCAG ATGGAGAAGA GATACAAGAC TCTCCAAGAC
    ACTCCAATCA ACTGCAATGA CATCTTTAAC CCTTCAGCTG AAGCAGGAGA TGAGGAGAAG
    AGCAGAGAGG AGAAAGAGCA GATCAAGACT GTTCTCACTA AAGGCATCGC TGGAATTGGA
    AAAACCGTCT CTGTGCAGAA GTTCATTCTG GACTGGGCCG AGGGAAAAGC CAATCAGGAT
    GTAGATTTCA TCTTTGTGCT TCCGTTTCGA GAGCTGAACT TGATTAAAGA TCATCAGTAC
    AGTCTTCACA CACTTCTGCT GGACTTTCAT CCTGAACTTC AGCATCTGGA CTCACAGATT
    TATGAGGAGT GTAAAGTCGT GTTCATCTTT GATGGTCTGG ATGAAAGCAG AATCACACTG
    ATGTTTTCAG ACTGTCAGAA AGTTTCTGAT GTGACTGAAG CTTCATCAGT GAGTGTGTTG
    ATGTCAAACC TCATGAAAGG AGATCTGCTT CCCTCCGCTC TCATCTGGAT CACCAGCAGA
    CCAGCAGCGG CCAATCAGAT CCCCTCAAAA CACATCAGCC TCGTGACGGA GATTCAGGGC
    TTCAATGACC CTCAGAAGGA GGAATATTTC AGGAAGAGAA TCAGAGATGA AGATCAAGCC
    AGCAGAATCA TCTCCCACAT CAGAAGAGCA AGAAGCCTCC ACATCATGTG CCACATACCC
    GTCTTCTGCT GGATCTCAGC CACTGTGCTT CAAACCATCC TGAGACAAGA TCTGAGTGCA
    GAAATCCCTC AAACTCTGAC TGAGATGTAC ATTCACTTCC TCCTCATTCA GACACACATG
    AGAAAGCAGA AGTATGAAGA GAGAGATCCA GAGAAACTCC TGCGGTTCAA CAGAGACGTG
    ATTGTGAAAC TTGCTGAACT GGCTTTCAAT CAGCTGATGA AGGGCAATGT GATGTTCTAC
    GAGGAGGACC TGATTGAGAG CAGGATAGAC GTCGCTGACG CCTCAGTGTA TTCTGGGATC
    TGCACTGAGA TCTTCAGAGA GGAATCTGTG ATTTATCAGA GGAAGGTTTA CAGCTTTGTT
    CATCTGAGCT TTCAGGAGTG TTTTGCAGCA CTGTATGTGT TTTACTGCTA TTTACACAAC
    AACACTGAGG TGATGAAGAT GTTCCTGACA GAAATATACA GAACTCAGAG TAAGAAAGTT
    TCTCTGGATG TGCTTCTGAA AGGAGTGATG ATTGAAGCCT TGAAAAGTAA AACTGGACAC
    CTGGATCTGT TCCTGCGATT TCTTCATGGC GTATCACTGG AGTCCAATCA GAGACTCTTA
    CAGGATCTGC TGAAACACAC AGAGAACGAC CCAGACAGCA TCAAGAAAAT AATCAAAAAC
    CTCAAACGAG GACAAAAAAA CAACGTCAGT CCTGAAAGAT GGATGAATCT CTCACACTGC
    TTGATGGAGA TGAAGGACAA TTCTGTGGTT AAAGAAATGC AAGCATTCTT AAAATCTAAA
    ACACAAAGAA AATGTCTTTC TCTTGCACAA TGTTCGACTT TGGCAAACAT GATCCTGATG
    TCAGAGGAGG TGCTGGATGA GCTAGACATT AAAAAGTACA ACATTAAATC AAAAGAAGGT
    CGATGGAGAC TGATACCCGC TGTGAGAAAC TGCAGAAAAG CTCTATTGTC AGAATGTAAT
    CTTGCAGATC AACACTGTGA AACTGTAGCT TCAGCTCTAC AATCATCAAA CAACCTCTTG
    AGAGAAGTGA ATCTGAGTCG CAATGAGCTG CAGGATTCAG GAGTGAAGCT GATCTGTGCT
    GGACTGAAGA GCCCAAACTG TCAACTCAAC GTTCTGAAAT TGTCAGGCTG TAATCTCACT
    AAACAGTCCT GTGAAACTGT GGCTGTGTCT CTACTTTCAT CAAACTGCCG CCTGAGAGAG
    CTGGACCTGA GTCGCAATGA CCTGCAGGAT TCAGGAGTGA AGCTGATCTG TGCTGGACTG
    AAAAGTGCAA ACTGTCAACT CAACACACTG AGACTGGCAA AATGTAACCT CACTGATCAG
    TGCTGCGAAA CTATATCTTC ATTTCTACAA TCATCAAACT CTGTCTTGAG AGAGCTGGAC
    ATAAGCAACA ATCGTCTGCA GGATTCAGGA GTGAAGCTGA TTTTTACTGC AATAAAGAGT
    GAGAACTGTC AACTCAACAT CCTGAGATTG TCAAGTTGTA ACCTCACTGA TCAGTGCTGT
    GAAACTTTGG CTTCATCTCT ACAATCATCA AACTGTGTCC TGAGAGAGCT GGACCTGAGT
    AACAATGAGC TGCAGGATTC AGGAGTGAAG CTGATCTGTG CTGGATTGAA GAGCCCAAAC
    TGTCAACTCA ACACACTGAG ATTGTCAGGC TGTAATCTCA CTGATCAGTG CTGTGAAACT
    GTGGCTTCAT CTCTACAATT ATCCAACTGC CCTCTGAGAG AGCTGGACCT GAGTCACAAT
    GATCTTCAGG GTTCAAGAGT GAAGCTGATC TGTGCTGGAC TGAAGAGCCC AAACTGTCAA
    CTCAACACAC TGAGATTATC TTACTGTTTG GTGTCAGAGG AAGGTTGTGC AGCTCTGGCT
    TCAGCTCTGA GCTCAAACCC GTCACACCTG AGAGAGCTGG ATCTGAGCTA CAATCACCCA
    GGAGAAGCAG GACTCAAACT GCTCTCTGAT CAGCTCAAAC ACCTCCACAA ACTCAATGTG
    GATCATGGAG GAGAATTCAG GACTGTATCA ACACTACACA AATACGCCTG TGAACTCACA
    CTGGATCCAA ACACAGCACA CACACGCCTC GCTCTGTCTG AGGAGAACAG AAAGGCGACG
    CGTGTGAAGG AGAGTCAGTC TTATCCTCGT CATCCAGACA GATTTGATAA GTTTCATCAG
    GTTATGAGTA AAGAGAGTCT GACTGGACGC TGTTACTGGG AGACTCAATG GAGCGGATAT
    GCTGAAATAT CAGTTTGTTA TAAAGGAATC AAGAGGAAAG GACGGAGTGA CTGTCGGTTT
    GGATGTAATG ATCAGTCCTG GAGACTTGAT TGCTCTGATC ACTCATTCTC TGTCTGTCAC
    AATAATGAAC TCACACACAT CTCAGCTCGT CCAGACTCCA GCAGGACAGT AGGAGTGTTT
    GTGGACGAGT CGAGCGGCTC TCTGTCCTTC TACAGTGTCT CTGATAAACT CACACACTTA
    CACACATTCA ACACCACATT CACCGACACA CTCCACGCTG GATTTAGACT TGGTTATAAA
    TCTTCAGTGT CTCTGTGTCG CATTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330450.1
    CDS160..4689
    Translation

    Target ORF information:

    RefSeq Version XM_021474775.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-214b16.3 (si:ch211-214b16.3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474775.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGGAAAGGA AAGCTAAACA TCCATGTGAA ATGAGTGTGT TTGAAGAGAG AGAGAGGGAG 
    GCTTTCATTC AGACTGTGAG AGCAGTTTCT CCATTATCTA GCTGTGTGTC TATGATGTGT
    AACCACTCCA TTGGTCTCCC CCCTGATCGC AGTGATGGAG CTGTTAGCTC TGACTTTGTC
    TGGGAAAGGA CCAGATCTCC ATTATCTAGC TGTGTGTCTA TGAAGAGCAA CAACTCTATG
    GGTCTCCCCC CTGATCTCAG TGATGGAGCT GTCAACTCTG AGTTCCTCAA GAGAAAGAGA
    GAAGAGTCTT CATTGTCTAG CAGCATGTTT ATAAAGAGTG ACCACCCCTT TGGTCTCCCC
    CTTTATCTCA GTGATAGAGC TGTGAACTCT GACTCTGTCT GGGAAAGGAC CAGATCTCCA
    TTATCTAGCT GTGTGTCTAT GAAGAGCAAC AACTCTATGG GTCTCCCCCC TGATCTCAGT
    GATGGAGCTG TCAACTCTGA GTTCCTCTGG GAAAGGACCA GATCTCCATT ATCTAGCTGT
    GTGTCTATGA AGAGCAACAA CTCTATGGGT CTCCCCCCTG ATCTCAGTGA TGGAGCTGTC
    AACTCTGAGT TCCTCAAGAG AAAGAGAGAA GAGTCTTCAT TGTCTAGCAG CATGTTTATA
    AAGAGTGACC ACCCCTTTGG TCTCCCCCTT TATCTCAGTG ATAGAGCTGT GAACTCTGAC
    TCTGTCTGGA AAAGGACCAG ATCTCCATTA TCTAGCTGTG TGTCTATGAA GAGCAACAAC
    TCTATGGGTC TCCCCCCTGA TCTCAGTGAT GGAGCTGTCA ACTCTGAGTT CCTCAAGAGA
    AAGAGAGAAG AGTCTTCATT GTCTAGCAGC ATGTCTATGA AGAGTGACCA CTACATTGAT
    CTCCCCCCAT GTAAACTCAT CAGCAATAGA AAGAGAGAGT CTTCTCCATT ATCTAGCTGT
    GTGTCTATGA AGAGTAATCA CTCCATGAGT CTCCCCCCTT ATCTCAGTGA TGGAGCTGTG
    AACTCTGACT CTGTATCTCC CTACTATCAG AAACACATCA GTCATTTTAA AACAGAGGCA
    TGCCTGATGA TTGATTCTCA ACAACTAGTG CATGATGATC TCCAGAGAGT CAAAGACCAG
    CACAGATTCA TCATGAAGAT CAAGTACGAG AGCTTATTTG AGGGAGTGAA ACTCCAGCAG
    AATCAAACCC TCCTGAACAG CATTTACACA CAGCTGTACA TCATAGAGGG AGAGAGTGAA
    GGAGTGAATG AAGAACATGA GGTGCTGCAG ATGGAGAAGA GATACAAGAC TCTCCAAGAC
    ACTCCAATCA ACTGCAATGA CATCTTTAAC CCTTCAGCTG AAGCAGGAGA TGAGGAGAAG
    AGCAGAGAGG AGAAAGAGCA GATCAAGACT GTTCTCACTA AAGGCATCGC TGGAATTGGA
    AAAACCGTCT CTGTGCAGAA GTTCATTCTG GACTGGGCCG AGGGAAAAGC CAATCAGGAT
    GTAGATTTCA TCTTTGTGCT TCCGTTTCGA GAGCTGAACT TGATTAAAGA TCATCAGTAC
    AGTCTTCACA CACTTCTGCT GGACTTTCAT CCTGAACTTC AGCATCTGGA CTCACAGATT
    TATGAGGAGT GTAAAGTCGT GTTCATCTTT GATGGTCTGG ATGAAAGCAG AATCACACTG
    ATGTTTTCAG ACTGTCAGAA AGTTTCTGAT GTGACTGAAG CTTCATCAGT GAGTGTGTTG
    ATGTCAAACC TCATGAAAGG AGATCTGCTT CCCTCCGCTC TCATCTGGAT CACCAGCAGA
    CCAGCAGCGG CCAATCAGAT CCCCTCAAAA CACATCAGCC TCGTGACGGA GATTCAGGGC
    TTCAATGACC CTCAGAAGGA GGAATATTTC AGGAAGAGAA TCAGAGATGA AGATCAAGCC
    AGCAGAATCA TCTCCCACAT CAGAAGAGCA AGAAGCCTCC ACATCATGTG CCACATACCC
    GTCTTCTGCT GGATCTCAGC CACTGTGCTT CAAACCATCC TGAGACAAGA TCTGAGTGCA
    GAAATCCCTC AAACTCTGAC TGAGATGTAC ATTCACTTCC TCCTCATTCA GACACACATG
    AGAAAGCAGA AGTATGAAGA GAGAGATCCA GAGAAACTCC TGCGGTTCAA CAGAGACGTG
    ATTGTGAAAC TTGCTGAACT GGCTTTCAAT CAGCTGATGA AGGGCAATGT GATGTTCTAC
    GAGGAGGACC TGATTGAGAG CAGGATAGAC GTCGCTGACG CCTCAGTGTA TTCTGGGATC
    TGCACTGAGA TCTTCAGAGA GGAATCTGTG ATTTATCAGA GGAAGGTTTA CAGCTTTGTT
    CATCTGAGCT TTCAGGAGTG TTTTGCAGCA CTGTATGTGT TTTACTGCTA TTTACACAAC
    AACACTGAGG TGATGAAGAT GTTCCTGACA GAAATATACA GAACTCAGAG TAAGAAAGTT
    TCTCTGGATG TGCTTCTGAA AGGAGTGATG ATTGAAGCCT TGAAAAGTAA AACTGGACAC
    CTGGATCTGT TCCTGCGATT TCTTCATGGC GTATCACTGG AGTCCAATCA GAGACTCTTA
    CAGGATCTGC TGAAACACAC AGAGAACGAC CCAGACAGCA TCAAGAAAAT AATCAAAAAC
    CTCAAACGAG GACAAAAAAA CAACGTCAGT CCTGAAAGAT GGATGAATCT CTCACACTGC
    TTGATGGAGA TGAAGGACAA TTCTGTGGTT AAAGAAATGC AAGCATTCTT AAAATCTAAA
    ACACAAAGAA AATGTCTTTC TCTTGCACAA TGTTCGACTT TGGCAAACAT GATCCTGATG
    TCAGAGGAGG TGCTGGATGA GCTAGACATT AAAAAGTACA ACATTAAATC AAAAGAAGGT
    CGATGGAGAC TGATACCCGC TGTGAGAAAC TGCAGAAAAG CTCTATTGTC AGAATGTAAT
    CTTGCAGATC AACACTGTGA AACTGTAGCT TCAGCTCTAC AATCATCAAA CAACCTCTTG
    AGAGAAGTGA ATCTGAGTCG CAATGAGCTG CAGGATTCAG GAGTGAAGCT GATCTGTGCT
    GGACTGAAGA GCCCAAACTG TCAACTCAAC GTTCTGAAAT TGTCAGGCTG TAATCTCACT
    AAACAGTCCT GTGAAACTGT GGCTGTGTCT CTACTTTCAT CAAACTGCCG CCTGAGAGAG
    CTGGACCTGA GTCGCAATGA CCTGCAGGAT TCAGGAGTGA AGCTGATCTG TGCTGGACTG
    AAAAGTGCAA ACTGTCAACT CAACACACTG AGACTGGCAA AATGTAACCT CACTGATCAG
    TGCTGCGAAA CTATATCTTC ATTTCTACAA TCATCAAACT CTGTCTTGAG AGAGCTGGAC
    ATAAGCAACA ATCGTCTGCA GGATTCAGGA GTGAAGCTGA TTTTTACTGC AATAAAGAGT
    GAGAACTGTC AACTCAACAT CCTGAGATTG TCAAGTTGTA ACCTCACTGA TCAGTGCTGT
    GAAACTTTGG CTTCATCTCT ACAATCATCA AACTGTGTCC TGAGAGAGCT GGACCTGAGT
    AACAATGAGC TGCAGGATTC AGGAGTGAAG CTGATCTGTG CTGGATTGAA GAGCCCAAAC
    TGTCAACTCA ACACACTGAG ATTGTCAGGC TGTAATCTCA CTGATCAGTG CTGTGAAACT
    GTGGCTTCAT CTCTACAATT ATCCAACTGC CCTCTGAGAG AGCTGGACCT GAGTCACAAT
    GATCTTCAGG GTTCAAGAGT GAAGCTGATC TGTGCTGGAC TGAAGAGCCC AAACTGTCAA
    CTCAACACAC TGAGATTATC TTACTGTTTG GTGTCAGAGG AAGGTTGTGC AGCTCTGGCT
    TCAGCTCTGA GCTCAAACCC GTCACACCTG AGAGAGCTGG ATCTGAGCTA CAATCACCCA
    GGAGAAGCAG GACTCAAACT GCTCTCTGAT CAGCTCAAAC ACCTCCACAA ACTCAATGTG
    GATCATGGAG GAGAATTCAG GACTGTATCA ACACTACACA AATACGCCTG TGAACTCACA
    CTGGATCCAA ACACAGCACA CACACGCCTC GCTCTGTCTG AGGAGAACAG AAAGGCGACG
    CGTGTGAAGG AGAGTCAGTC TTATCCTCGT CATCCAGACA GATTTGATAA GTTTCATCAG
    GTTATGAGTA AAGAGAGTCT GACTGGACGC TGTTACTGGG AGACTCAATG GAGCGGATAT
    GCTGAAATAT CAGTTTGTTA TAAAGGAATC AAGAGGAAAG GACGGAGTGA CTGTCGGTTT
    GGATGTAATG ATCAGTCCTG GAGACTTGAT TGCTCTGATC ACTCATTCTC TGTCTGTCAC
    AATAATGAAC TCACACACAT CTCAGCTCGT CCAGACTCCA GCAGGACAGT AGGAGTGTTT
    GTGGACGAGT CGAGCGGCTC TCTGTCCTTC TACAGTGTCT CTGATAAACT CACACACTTA
    CACACATTCA ACACCACATT CACCGACACA CTCCACGCTG GATTTAGACT TGGTTATAAA
    TCTTCAGTGT CTCTGTGTCG CATTAAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44300
    Clone ID Related Accession (Same CDS sequence) XM_021474771.1
    Accession Version XM_021474771.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4641bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 3 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAAAGGA AAGCTAAACA TCCATGTGAA ATGAGTGTGT TTGAAGAGAG AGAGAGGGAG 
    GCTTTCATTC AGACTGTGAG AGCAGTTTCT CCATTATCTA GCTGTGTGTC TATGATGTGT
    AACCACTCCA TTGGTCTCCC CCCTGATCGC AGTGATGGAG CTGTTAGCTC TGACTTTGTC
    TGGGAAAGGA CCAGATCTCC ATTATCTAGC TGTGTGTCTA TGAAGAGCAA CAACTCTATG
    GGTCTCCCCC CTGATCTCAG TGATGGAGCT GTCAACTCTG AGTTCCTCAA GAGAAAGAGA
    GAAGAGTCTT CATTGTCTAG CAGCATGTTT ATAAAGAGTG ACCACCCCTT TGGTCTCCCC
    CTTTATCTCA GTGATAGAGC TGTGAACTCT GACTCTGTCT GGGAAAGGAC CAGATCTCCA
    TTATCTAGCT GTGTGTCTAT GAAGAGCAAC AACTCTATGG GTCTCCCCCC TGATCTCAGT
    GATGGAGCTG TCAACTCTGA GTTCCTCAAG AGAAAGAGAG AAGAGTCTTC ATTGTCTAGC
    AGCATGTTTA TAAAGAGTGA CCACCCCTTT GGTCTCCCCC TTTATCTCAG TGATAGAGCT
    GTGAACTCTG ACTCTGTCTG GGAAAGGACC AGATCTCCAT TATCTAGCTG TGTGTCTATG
    AAGAGCAACA ACTCTATGGG TCTCCCCCCT GATCTCAGTG ATGGAGCTGT CAACTCTGAG
    TTCCTCAAGA GAAAGAGAGA AGAGTCTTCA TTGTCTAGCA GCATGTTTAT AAAGAGTGAC
    CACCCCTTTG GTCTCCCCCT TTATCTCAGT GATAGAGCTG TGAACTCTGA CTCTGTCTGG
    AAAAGGACCA GATCTCCATT ATCTAGCTGT GTGTCTATGA AGAGCAACAA CTCTATGGGT
    CTCCCCCCTG ATCTCAGTGA TGGAGCTGTC AACTCTGAGT TCCTCAAGAG AAAGAGAGAA
    GAGTCTTCAT TGTCTAGCAG CATGTCTATG AAGAGTGACC ACTACATTGA TCTCCCCCCA
    TGTAAACTCA TCAGCAATAG AAAGAGAGAG TCTTCTCCAT TATCTAGCTG TGTGTCTATG
    AAGAGTAATC ACTCCATGAG TCTCCCCCCT TATCTCAGTG ATGGAGCTGT GAACTCTGAC
    TCTGTATCTC CCTACTATCA GAAACACATC AGTCATTTTA AAACAGAGGC ATGCCTGATG
    ATTGATTCTC AACAACTAGT GCATGATGAT CTCCAGAGAG TCAAAGACCA GCACAGATTC
    ATCATGAAGA TCAAGTACGA GAGCTTATTT GAGGGAGTGA AACTCCAGCA GAATCAAACC
    CTCCTGAACA GCATTTACAC ACAGCTGTAC ATCATAGAGG GAGAGAGTGA AGGAGTGAAT
    GAAGAACATG AGGTGCTGCA GATGGAGAAG AGATACAAGA CTCTCCAAGA CACTCCAATC
    AACTGCAATG ACATCTTTAA CCCTTCAGCT GAAGCAGGAG ATGAGGAGAA GAGCAGAGAG
    GAGAAAGAGC AGATCAAGAC TGTTCTCACT AAAGGCATCG CTGGAATTGG AAAAACCGTC
    TCTGTGCAGA AGTTCATTCT GGACTGGGCC GAGGGAAAAG CCAATCAGGA TGTAGATTTC
    ATCTTTGTGC TTCCGTTTCG AGAGCTGAAC TTGATTAAAG ATCATCAGTA CAGTCTTCAC
    ACACTTCTGC TGGACTTTCA TCCTGAACTT CAGCATCTGG ACTCACAGAT TTATGAGGAG
    TGTAAAGTCG TGTTCATCTT TGATGGTCTG GATGAAAGCA GAATCACACT GATGTTTTCA
    GACTGTCAGA AAGTTTCTGA TGTGACTGAA GCTTCATCAG TGAGTGTGTT GATGTCAAAC
    CTCATGAAAG GAGATCTGCT TCCCTCCGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCG
    GCCAATCAGA TCCCCTCAAA ACACATCAGC CTCGTGACGG AGATTCAGGG CTTCAATGAC
    CCTCAGAAGG AGGAATATTT CAGGAAGAGA ATCAGAGATG AAGATCAAGC CAGCAGAATC
    ATCTCCCACA TCAGAAGAGC AAGAAGCCTC CACATCATGT GCCACATACC CGTCTTCTGC
    TGGATCTCAG CCACTGTGCT TCAAACCATC CTGAGACAAG ATCTGAGTGC AGAAATCCCT
    CAAACTCTGA CTGAGATGTA CATTCACTTC CTCCTCATTC AGACACACAT GAGAAAGCAG
    AAGTATGAAG AGAGAGATCC AGAGAAACTC CTGCGGTTCA ACAGAGACGT GATTGTGAAA
    CTTGCTGAAC TGGCTTTCAA TCAGCTGATG AAGGGCAATG TGATGTTCTA CGAGGAGGAC
    CTGATTGAGA GCAGGATAGA CGTCGCTGAC GCCTCAGTGT ATTCTGGGAT CTGCACTGAG
    ATCTTCAGAG AGGAATCTGT GATTTATCAG AGGAAGGTTT ACAGCTTTGT TCATCTGAGC
    TTTCAGGAGT GTTTTGCAGC ACTGTATGTG TTTTACTGCT ATTTACACAA CAACACTGAG
    GTGATGAAGA TGTTCCTGAC AGAAATATAC AGAACTCAGA GTAAGAAAGT TTCTCTGGAT
    GTGCTTCTGA AAGGAGTGAT GATTGAAGCC TTGAAAAGTA AAACTGGACA CCTGGATCTG
    TTCCTGCGAT TTCTTCATGG CGTATCACTG GAGTCCAATC AGAGACTCTT ACAGGATCTG
    CTGAAACACA CAGAGAACGA CCCAGACAGC ATCAAGAAAA TAATCAAAAA CCTCAAACGA
    GGACAAAAAA ACAACGTCAG TCCTGAAAGA TGGATGAATC TCTCACACTG CTTGATGGAG
    ATGAAGGACA ATTCTGTGGT TAAAGAAATG CAAGCATTCT TAAAATCTAA AACACAAAGA
    AAATGTCTTT CTCTTGCACA ATGTTCGACT TTGGCAAACA TGATCCTGAT GTCAGAGGAG
    GTGCTGGATG AGCTAGACAT TAAAAAGTAC AACATTAAAT CAAAAGAAGG TCGATGGAGA
    CTGATACCCG CTGTGAGAAA CTGCAGAAAA GCTCTATTGT CAGAATGTAA TCTTGCAGAT
    CAACACTGTG AAACTGTAGC TTCAGCTCTA CAATCATCAA ACAACCTCTT GAGAGAAGTG
    AATCTGAGTC GCAATGAGCT GCAGGATTCA GGAGTGAAGC TGATCTGTGC TGGACTGAAG
    AGCCCAAACT GTCAACTCAA CGTTCTGAAA TTGTCAGGCT GTAATCTCAC TAAACAGTCC
    TGTGAAACTG TGGCTGTGTC TCTACTTTCA TCAAACTGCC GCCTGAGAGA GCTGGACCTG
    AGTCGCAATG ACCTGCAGGA TTCAGGAGTG AAGCTGATCT GTGCTGGACT GAAAAGTGCA
    AACTGTCAAC TCAACACACT GAGACTGGCA AAATGTAACC TCACTGATCA GTGCTGCGAA
    ACTATATCTT CATTTCTACA ATCATCAAAC TCTGTCTTGA GAGAGCTGGA CATAAGCAAC
    AATCGTCTGC AGGATTCAGG AGTGAAGCTG ATTTTTACTG CAATAAAGAG TGAGAACTGT
    CAACTCAACA TCCTGAGATT GTCAAGTTGT AACCTCACTG ATCAGTGCTG TGAAACTTTG
    GCTTCATCTC TACAATCATC AAACTGTGTC CTGAGAGAGC TGGACCTGAG TAACAATGAG
    CTGCAGGATT CAGGAGTGAA GCTGATCTGT GCTGGATTGA AGAGCCCAAA CTGTCAACTC
    AACACACTGA GATTGTCAGG CTGTAATCTC ACTGATCAGT GCTGTGAAAC TGTGGCTTCA
    TCTCTACAAT TATCCAACTG CCCTCTGAGA GAGCTGGACC TGAGTCACAA TGATCTTCAG
    GGTTCAAGAG TGAAGCTGAT CTGTGCTGGA CTGAAGAGCC CAAACTGTCA ACTCAACACA
    CTGAGATTAT CTTACTGTTT GGTGTCAGAG GAAGGTTGTG CAGCTCTGGC TTCAGCTCTG
    AGCTCAAACC CGTCACACCT GAGAGAGCTG GATCTGAGCT ACAATCACCC AGGAGAAGCA
    GGACTCAAAC TGCTCTCTGA TCAGCTCAAA CACCTCCACA AACTCAATGT GGATCATGGA
    GGAGAATTCA GGACTGTATC AACACTACAC AAATACGCCT GTGAACTCAC ACTGGATCCA
    AACACAGCAC ACACACGCCT CGCTCTGTCT GAGGAGAACA GAAAGGCGAC GCGTGTGAAG
    GAGAGTCAGT CTTATCCTCG TCATCCAGAC AGATTTGATA AGTTTCATCA GGTTATGAGT
    AAAGAGAGTC TGACTGGACG CTGTTACTGG GAGACTCAAT GGAGCGGATA TGCTGAAATA
    TCAGTTTGTT ATAAAGGAAT CAAGAGGAAA GGACGGAGTG ACTGTCGGTT TGGATGTAAT
    GATCAGTCCT GGAGACTTGA TTGCTCTGAT CACTCATTCT CTGTCTGTCA CAATAATGAA
    CTCACACACA TCTCAGCTCG TCCAGACTCC AGCAGGACAG TAGGAGTGTT TGTGGACGAG
    TCGAGCGGCT CTCTGTCCTT CTACAGTGTC TCTGATAAAC TCACACACTT ACACACATTC
    AACACCACAT TCACCGACAC ACTCCACGCT GGATTTAGAC TTGGTTATAA ATCTTCAGTG
    TCTCTGTGTC GCATTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330446.1
    CDS160..4800
    Translation

    Target ORF information:

    RefSeq Version XM_021474771.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio si:ch211-214b16.3 (si:ch211-214b16.3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021474771.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGGAAAGGA AAGCTAAACA TCCATGTGAA ATGAGTGTGT TTGAAGAGAG AGAGAGGGAG 
    GCTTTCATTC AGACTGTGAG AGCAGTTTCT CCATTATCTA GCTGTGTGTC TATGATGTGT
    AACCACTCCA TTGGTCTCCC CCCTGATCGC AGTGATGGAG CTGTTAGCTC TGACTTTGTC
    TGGGAAAGGA CCAGATCTCC ATTATCTAGC TGTGTGTCTA TGAAGAGCAA CAACTCTATG
    GGTCTCCCCC CTGATCTCAG TGATGGAGCT GTCAACTCTG AGTTCCTCAA GAGAAAGAGA
    GAAGAGTCTT CATTGTCTAG CAGCATGTTT ATAAAGAGTG ACCACCCCTT TGGTCTCCCC
    CTTTATCTCA GTGATAGAGC TGTGAACTCT GACTCTGTCT GGGAAAGGAC CAGATCTCCA
    TTATCTAGCT GTGTGTCTAT GAAGAGCAAC AACTCTATGG GTCTCCCCCC TGATCTCAGT
    GATGGAGCTG TCAACTCTGA GTTCCTCAAG AGAAAGAGAG AAGAGTCTTC ATTGTCTAGC
    AGCATGTTTA TAAAGAGTGA CCACCCCTTT GGTCTCCCCC TTTATCTCAG TGATAGAGCT
    GTGAACTCTG ACTCTGTCTG GGAAAGGACC AGATCTCCAT TATCTAGCTG TGTGTCTATG
    AAGAGCAACA ACTCTATGGG TCTCCCCCCT GATCTCAGTG ATGGAGCTGT CAACTCTGAG
    TTCCTCAAGA GAAAGAGAGA AGAGTCTTCA TTGTCTAGCA GCATGTTTAT AAAGAGTGAC
    CACCCCTTTG GTCTCCCCCT TTATCTCAGT GATAGAGCTG TGAACTCTGA CTCTGTCTGG
    AAAAGGACCA GATCTCCATT ATCTAGCTGT GTGTCTATGA AGAGCAACAA CTCTATGGGT
    CTCCCCCCTG ATCTCAGTGA TGGAGCTGTC AACTCTGAGT TCCTCAAGAG AAAGAGAGAA
    GAGTCTTCAT TGTCTAGCAG CATGTCTATG AAGAGTGACC ACTACATTGA TCTCCCCCCA
    TGTAAACTCA TCAGCAATAG AAAGAGAGAG TCTTCTCCAT TATCTAGCTG TGTGTCTATG
    AAGAGTAATC ACTCCATGAG TCTCCCCCCT TATCTCAGTG ATGGAGCTGT GAACTCTGAC
    TCTGTATCTC CCTACTATCA GAAACACATC AGTCATTTTA AAACAGAGGC ATGCCTGATG
    ATTGATTCTC AACAACTAGT GCATGATGAT CTCCAGAGAG TCAAAGACCA GCACAGATTC
    ATCATGAAGA TCAAGTACGA GAGCTTATTT GAGGGAGTGA AACTCCAGCA GAATCAAACC
    CTCCTGAACA GCATTTACAC ACAGCTGTAC ATCATAGAGG GAGAGAGTGA AGGAGTGAAT
    GAAGAACATG AGGTGCTGCA GATGGAGAAG AGATACAAGA CTCTCCAAGA CACTCCAATC
    AACTGCAATG ACATCTTTAA CCCTTCAGCT GAAGCAGGAG ATGAGGAGAA GAGCAGAGAG
    GAGAAAGAGC AGATCAAGAC TGTTCTCACT AAAGGCATCG CTGGAATTGG AAAAACCGTC
    TCTGTGCAGA AGTTCATTCT GGACTGGGCC GAGGGAAAAG CCAATCAGGA TGTAGATTTC
    ATCTTTGTGC TTCCGTTTCG AGAGCTGAAC TTGATTAAAG ATCATCAGTA CAGTCTTCAC
    ACACTTCTGC TGGACTTTCA TCCTGAACTT CAGCATCTGG ACTCACAGAT TTATGAGGAG
    TGTAAAGTCG TGTTCATCTT TGATGGTCTG GATGAAAGCA GAATCACACT GATGTTTTCA
    GACTGTCAGA AAGTTTCTGA TGTGACTGAA GCTTCATCAG TGAGTGTGTT GATGTCAAAC
    CTCATGAAAG GAGATCTGCT TCCCTCCGCT CTCATCTGGA TCACCAGCAG ACCAGCAGCG
    GCCAATCAGA TCCCCTCAAA ACACATCAGC CTCGTGACGG AGATTCAGGG CTTCAATGAC
    CCTCAGAAGG AGGAATATTT CAGGAAGAGA ATCAGAGATG AAGATCAAGC CAGCAGAATC
    ATCTCCCACA TCAGAAGAGC AAGAAGCCTC CACATCATGT GCCACATACC CGTCTTCTGC
    TGGATCTCAG CCACTGTGCT TCAAACCATC CTGAGACAAG ATCTGAGTGC AGAAATCCCT
    CAAACTCTGA CTGAGATGTA CATTCACTTC CTCCTCATTC AGACACACAT GAGAAAGCAG
    AAGTATGAAG AGAGAGATCC AGAGAAACTC CTGCGGTTCA ACAGAGACGT GATTGTGAAA
    CTTGCTGAAC TGGCTTTCAA TCAGCTGATG AAGGGCAATG TGATGTTCTA CGAGGAGGAC
    CTGATTGAGA GCAGGATAGA CGTCGCTGAC GCCTCAGTGT ATTCTGGGAT CTGCACTGAG
    ATCTTCAGAG AGGAATCTGT GATTTATCAG AGGAAGGTTT ACAGCTTTGT TCATCTGAGC
    TTTCAGGAGT GTTTTGCAGC ACTGTATGTG TTTTACTGCT ATTTACACAA CAACACTGAG
    GTGATGAAGA TGTTCCTGAC AGAAATATAC AGAACTCAGA GTAAGAAAGT TTCTCTGGAT
    GTGCTTCTGA AAGGAGTGAT GATTGAAGCC TTGAAAAGTA AAACTGGACA CCTGGATCTG
    TTCCTGCGAT TTCTTCATGG CGTATCACTG GAGTCCAATC AGAGACTCTT ACAGGATCTG
    CTGAAACACA CAGAGAACGA CCCAGACAGC ATCAAGAAAA TAATCAAAAA CCTCAAACGA
    GGACAAAAAA ACAACGTCAG TCCTGAAAGA TGGATGAATC TCTCACACTG CTTGATGGAG
    ATGAAGGACA ATTCTGTGGT TAAAGAAATG CAAGCATTCT TAAAATCTAA AACACAAAGA
    AAATGTCTTT CTCTTGCACA ATGTTCGACT TTGGCAAACA TGATCCTGAT GTCAGAGGAG
    GTGCTGGATG AGCTAGACAT TAAAAAGTAC AACATTAAAT CAAAAGAAGG TCGATGGAGA
    CTGATACCCG CTGTGAGAAA CTGCAGAAAA GCTCTATTGT CAGAATGTAA TCTTGCAGAT
    CAACACTGTG AAACTGTAGC TTCAGCTCTA CAATCATCAA ACAACCTCTT GAGAGAAGTG
    AATCTGAGTC GCAATGAGCT GCAGGATTCA GGAGTGAAGC TGATCTGTGC TGGACTGAAG
    AGCCCAAACT GTCAACTCAA CGTTCTGAAA TTGTCAGGCT GTAATCTCAC TAAACAGTCC
    TGTGAAACTG TGGCTGTGTC TCTACTTTCA TCAAACTGCC GCCTGAGAGA GCTGGACCTG
    AGTCGCAATG ACCTGCAGGA TTCAGGAGTG AAGCTGATCT GTGCTGGACT GAAAAGTGCA
    AACTGTCAAC TCAACACACT GAGACTGGCA AAATGTAACC TCACTGATCA GTGCTGCGAA
    ACTATATCTT CATTTCTACA ATCATCAAAC TCTGTCTTGA GAGAGCTGGA CATAAGCAAC
    AATCGTCTGC AGGATTCAGG AGTGAAGCTG ATTTTTACTG CAATAAAGAG TGAGAACTGT
    CAACTCAACA TCCTGAGATT GTCAAGTTGT AACCTCACTG ATCAGTGCTG TGAAACTTTG
    GCTTCATCTC TACAATCATC AAACTGTGTC CTGAGAGAGC TGGACCTGAG TAACAATGAG
    CTGCAGGATT CAGGAGTGAA GCTGATCTGT GCTGGATTGA AGAGCCCAAA CTGTCAACTC
    AACACACTGA GATTGTCAGG CTGTAATCTC ACTGATCAGT GCTGTGAAAC TGTGGCTTCA
    TCTCTACAAT TATCCAACTG CCCTCTGAGA GAGCTGGACC TGAGTCACAA TGATCTTCAG
    GGTTCAAGAG TGAAGCTGAT CTGTGCTGGA CTGAAGAGCC CAAACTGTCA ACTCAACACA
    CTGAGATTAT CTTACTGTTT GGTGTCAGAG GAAGGTTGTG CAGCTCTGGC TTCAGCTCTG
    AGCTCAAACC CGTCACACCT GAGAGAGCTG GATCTGAGCT ACAATCACCC AGGAGAAGCA
    GGACTCAAAC TGCTCTCTGA TCAGCTCAAA CACCTCCACA AACTCAATGT GGATCATGGA
    GGAGAATTCA GGACTGTATC AACACTACAC AAATACGCCT GTGAACTCAC ACTGGATCCA
    AACACAGCAC ACACACGCCT CGCTCTGTCT GAGGAGAACA GAAAGGCGAC GCGTGTGAAG
    GAGAGTCAGT CTTATCCTCG TCATCCAGAC AGATTTGATA AGTTTCATCA GGTTATGAGT
    AAAGAGAGTC TGACTGGACG CTGTTACTGG GAGACTCAAT GGAGCGGATA TGCTGAAATA
    TCAGTTTGTT ATAAAGGAAT CAAGAGGAAA GGACGGAGTG ACTGTCGGTT TGGATGTAAT
    GATCAGTCCT GGAGACTTGA TTGCTCTGAT CACTCATTCT CTGTCTGTCA CAATAATGAA
    CTCACACACA TCTCAGCTCG TCCAGACTCC AGCAGGACAG TAGGAGTGTT TGTGGACGAG
    TCGAGCGGCT CTCTGTCCTT CTACAGTGTC TCTGATAAAC TCACACACTT ACACACATTC
    AACACCACAT TCACCGACAC ACTCCACGCT GGATTTAGAC TTGGTTATAA ATCTTCAGTG
    TCTCTGTGTC GCATTAAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa58170
    Clone ID Related Accession (Same CDS sequence) XM_017354691.1
    Accession Version XM_017354691.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3705bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NACHT, LRR and PYD domains-containing protein 12
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007114.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 9% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATTGATT CTCAACAACT AGTGCATGAT GATCTCCAGA GAGTCAAAGA CCAGCACAGA 
    TTCATCATGA AGATCAAGTA CGAGAGCTTA TTTGAGGGAG TGAAACTCCA GCAGAATCAA
    ACCCTCCTGA ACAGCATTTA CACACAGCTG TACATCATAG AGGGAGAGAG TGAAGGAGTG
    AATGAAGAAC ATGAGGTGCT GCAGATGGAG AAGAGATACA AGACTCTCCA AGACACTCCA
    ATCAACTGCA ATGACATCTT TAACCCTTCA GCTGAAGCAG GAGATGAGGA GAAGAGCAGA
    GAGGAGAAAG AGCAGATCAA GACTGATGTA GATTTCATCT TTGTGCTTCC GTTTCGAGAG
    CTGAACTTGA TTAAAGATCA TCAGTACAGT CTTCACACAC TTCTGCTGGA CTTTCATCCT
    GAACTTCAGC ATCTGGACTC ACAGATTTAT GAGGAGTGTA AAGTCGTGTT CATCTTTGAT
    GGTCTGGATG AAAGCAGAAT CACACTGATG TTTTCAGACT GTCAGAAAGT TTCTGATGTG
    ACTGAAGCTT CATCAGTGAG TGTGTTGATG TCAAACCTCA TGAAAGGAGA TCTGCTTCCC
    TCCGCTCTCA TCTGGATCAC CAGCAGACCA GCAGCGGCCA ATCAGATCCC CTCAAAACAC
    ATCAGCCTCG TGACGGAGAT TCAGGGCTTC AATGACCCTC AGAAGGAGGA ATATTTCAGG
    AAGAGAATCA GAGATGAAGA TCAAGCCAGC AGAATCATCT CCCACATCAG AAGAGCAAGA
    AGCCTCCACA TCATGTGCCA CATACCCGTC TTCTGCTGGA TCTCAGCCAC TGTGCTTCAA
    ACCATCCTGA GACAAGATCT GAGTGCAGAA ATCCCTCAAA CTCTGACTGA GATGTACATT
    CACTTCCTCC TCATTCAGAC ACACATGAGA AAGCAGAAGT ATGAAGAGAG AGATCCAGAG
    AAACTCCTGC GGTTCAACAG AGACGTGATT GTGAAACTTG CTGAACTGGC TTTCAATCAG
    CTGATGAAGG GCAATGTGAT GTTCTACGAG GAGGACCTGA TTGAGAGCAG GATAGACGTC
    GCTGACGCCT CAGTGTATTC TGGGATCTGC ACTGAGATCT TCAGAGAGGA ATCTGTGATT
    TATCAGAGGA AGGTTTACAG CTTTGTTCAT CTGAGCTTTC AGGAGTGTTT TGCAGCACTG
    TATGTGTTTT ACTGCTATTT ACACAACAAC ACTGAGGTGA TGAAGATGTT CCTGACAGAA
    ATATACAGAA CTCAGAGTAA GAAAGTTTCT CTGGATGTGC TTCTGAAAGG AGTGATGATT
    GAAGCCTTGA AAAGTAAAAC TGGACACCTG GATCTGTTCC TGCGATTTCT TCATGGCGTA
    TCACTGGAGT CCAATCAGAG ACTCTTACAG GATCTGCTGA AACACACAGA GAACGACCCA
    GACAGCATCA AGAAAATAAT CAAAAACCTC AAACGAGGAC AAAAAAACAA CGTCAGTCCT
    GAAAGATGGA TGAATCTCTC ACACTGCTTG ATGGAGATGA AGGACAATTC TGTGGTTAAA
    GAAATGCAAG CATTCTTAAA ATCTAAAACA CAAAGAAAAT GTCTTTCTCT TGCACAATGT
    TCGACTTTGG CAAACATGAT CCTGATGTCA GAGGAGGTGC TGGATGAGCT AGACATTAAA
    AAGTACAACA TTAAATCAAA AGAAGGTCGA TGGAGACTGA TACCCGCTGT GAGAAACTGC
    AGAAAAGCTC TATTGTCAGA ATGTAATCTT GCAGATCAAC ACTGTGAAAC TGTAGCTTCA
    GCTCTACAAT CATCAAACAA CCTCTTGAGA GAAGTGAATC TGAGTCGCAA TGAGCTGCAG
    GATTCAGGAG TGAAGCTGAT CTGTGCTGGA CTGAAGAGCC CAAACTGTCA ACTCAACGTT
    CTGAAATTGT CAGGCTGTAA TCTCACTAAA CAGTCCTGTG AAACTGTGGC TGTGTCTCTA
    CTTTCATCAA ACTGCCGCCT GAGAGAGCTG GACCTGAGTC GCAATGACCT GCAGGATTCA
    GGAGTGAAGC TGATCTGTGC TGGACTGAAA AGTGCAAACT GTCAACTCAA CACACTGAGA
    CTGGCAAAAT GTAACCTCAC TGATCAGTGC TGCGAAACTA TATCTTCATT TCTACAATCA
    TCAAACTCTG TCTTGAGAGA GCTGGACATA AGCAACAATC GTCTGCAGGA TTCAGGAGTG
    AAGCTGATTT TTACTGCAAT AAAGAGTGAG AACTGTCAAC TCAACATCCT GAGATTGTCA
    AGTTGTAACC TCACTGATCA GTGCTGTGAA ACTTTGGCTT CATCTCTACA ATCATCAAAC
    TGTGTCCTGA GAGAGCTGGA CCTGAGTAAC AATGAGCTGC AGGATTCAGG AGTGAAGCTG
    ATCTGTGCTG GATTGAAGAG CCCAAACTGT CAACTCAACA CACTGAGATT GTCAGGCTGT
    AATCTCACTG ATCAGTGCTG TGAAACTGTG GCTTCATCTC TACAATTATC CAACTGCCCT
    CTGAGAGAGC TGGACCTGAG TCACAATGAT CTTCAGGGTT CAAGAGTGAA GCTGATCTGT
    GCTGGACTGA AGAGCCCAAA CTGTCAACTC AACACACTGA GACTGGCAAA ATGCAACCTC
    ACTGATGAGT GCTGTGAAAC TGTGGCTTCA TCTCTACAAT CATTAAACTG TGTCCTGAGA
    GAGCTGGACC TAAACAGCAA TTCTCTGCAG GATTCAGGAG TGAAGCTGAT TTTTACCGCA
    ATAAAGAGTG AGAACTGCCA GCTCAACACA CTGAGAATGT CAGACTGTAA TCTCACTGAT
    CAGTGCTGTG AAACTGTGGC TTCAGCTCTA CAATCATCAA ACTGCCCCCT GAGTGAGCTG
    GACCTGAGTA ATAATGAGCT GCAGGATTCA GGAGTGAAGC TGATCTGTGC TGGATTAAAG
    AGCCCAAACT GTCAACTCAA TATACTGAGA TTATCTTACT GTTTGGTGTC AGAGGAAGGT
    TGTGCAGCTC TGGCTTCAGC TCTGAGCTCA AACCCGTCAC ACCTGAGAGA GCTGGATCTG
    AGCTACAATC ACCCAGGAGA AGCAGGACTC AAACTGCTCT CTGATCAGCT CAAACACCTC
    CACAAACTCA ATGTGGATCA TGGAGGAGAA TTCAGGACTG TATCAACACT ACACAAATAC
    GCCTGTGAAC TCACACTGGA TCCAAACACA GCACACACAC GCCTCGCTCT GTCTGAGGAG
    AACAGAAAGG CGACGCGTGT GAAGGAGAGT CAGTCTTATC CTCGTCATCC AGACAGATTT
    GATAAGTTTC ATCAGGTTAT GAGTAAAGAG AGTCTGACTG GACGCTGTTA CTGGGAGACT
    CAATGGAGCG GATATGCTGA AATATCAGTT TGTTATAAAG GAATCAAGAG GAAAGGACGG
    AGTGACTGTC GGTTTGGATG TAATGATCAG TCCTGGAGAC TTGATTGCTC TGATCACTCA
    TTCTCTGTCT GTCACAATAA TGAACTCACA CACATCTCAG CTCGTCCAGA CTCCAGCAGG
    ACAGTAGGAG TGTTTGTGGA CGAGTCGAGC GGCTCTCTGT CCTTCTACAG TGTCTCTGAT
    AAACTCACAC ACTTACACAC ATTCAACACC ACATTCACCG ACACACTCCA CGCTGGATTT
    AGACTTGGTT ATAAATCTTC AGTGTCTCTG TGTCGCATTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017210180.1
    CDS1..3705
    Translation

    Target ORF information:

    RefSeq Version XM_017354691.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio si:ch211-214b16.3 (si:ch211-214b16.3), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017354691.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGATTGATT CTCAACAACT AGTGCATGAT GATCTCCAGA GAGTCAAAGA CCAGCACAGA 
    TTCATCATGA AGATCAAGTA CGAGAGCTTA TTTGAGGGAG TGAAACTCCA GCAGAATCAA
    ACCCTCCTGA ACAGCATTTA CACACAGCTG TACATCATAG AGGGAGAGAG TGAAGGAGTG
    AATGAAGAAC ATGAGGTGCT GCAGATGGAG AAGAGATACA AGACTCTCCA AGACACTCCA
    ATCAACTGCA ATGACATCTT TAACCCTTCA GCTGAAGCAG GAGATGAGGA GAAGAGCAGA
    GAGGAGAAAG AGCAGATCAA GACTGATGTA GATTTCATCT TTGTGCTTCC GTTTCGAGAG
    CTGAACTTGA TTAAAGATCA TCAGTACAGT CTTCACACAC TTCTGCTGGA CTTTCATCCT
    GAACTTCAGC ATCTGGACTC ACAGATTTAT GAGGAGTGTA AAGTCGTGTT CATCTTTGAT
    GGTCTGGATG AAAGCAGAAT CACACTGATG TTTTCAGACT GTCAGAAAGT TTCTGATGTG
    ACTGAAGCTT CATCAGTGAG TGTGTTGATG TCAAACCTCA TGAAAGGAGA TCTGCTTCCC
    TCCGCTCTCA TCTGGATCAC CAGCAGACCA GCAGCGGCCA ATCAGATCCC CTCAAAACAC
    ATCAGCCTCG TGACGGAGAT TCAGGGCTTC AATGACCCTC AGAAGGAGGA ATATTTCAGG
    AAGAGAATCA GAGATGAAGA TCAAGCCAGC AGAATCATCT CCCACATCAG AAGAGCAAGA
    AGCCTCCACA TCATGTGCCA CATACCCGTC TTCTGCTGGA TCTCAGCCAC TGTGCTTCAA
    ACCATCCTGA GACAAGATCT GAGTGCAGAA ATCCCTCAAA CTCTGACTGA GATGTACATT
    CACTTCCTCC TCATTCAGAC ACACATGAGA AAGCAGAAGT ATGAAGAGAG AGATCCAGAG
    AAACTCCTGC GGTTCAACAG AGACGTGATT GTGAAACTTG CTGAACTGGC TTTCAATCAG
    CTGATGAAGG GCAATGTGAT GTTCTACGAG GAGGACCTGA TTGAGAGCAG GATAGACGTC
    GCTGACGCCT CAGTGTATTC TGGGATCTGC ACTGAGATCT TCAGAGAGGA ATCTGTGATT
    TATCAGAGGA AGGTTTACAG CTTTGTTCAT CTGAGCTTTC AGGAGTGTTT TGCAGCACTG
    TATGTGTTTT ACTGCTATTT ACACAACAAC ACTGAGGTGA TGAAGATGTT CCTGACAGAA
    ATATACAGAA CTCAGAGTAA GAAAGTTTCT CTGGATGTGC TTCTGAAAGG AGTGATGATT
    GAAGCCTTGA AAAGTAAAAC TGGACACCTG GATCTGTTCC TGCGATTTCT TCATGGCGTA
    TCACTGGAGT CCAATCAGAG ACTCTTACAG GATCTGCTGA AACACACAGA GAACGACCCA
    GACAGCATCA AGAAAATAAT CAAAAACCTC AAACGAGGAC AAAAAAACAA CGTCAGTCCT
    GAAAGATGGA TGAATCTCTC ACACTGCTTG ATGGAGATGA AGGACAATTC TGTGGTTAAA
    GAAATGCAAG CATTCTTAAA ATCTAAAACA CAAAGAAAAT GTCTTTCTCT TGCACAATGT
    TCGACTTTGG CAAACATGAT CCTGATGTCA GAGGAGGTGC TGGATGAGCT AGACATTAAA
    AAGTACAACA TTAAATCAAA AGAAGGTCGA TGGAGACTGA TACCCGCTGT GAGAAACTGC
    AGAAAAGCTC TATTGTCAGA ATGTAATCTT GCAGATCAAC ACTGTGAAAC TGTAGCTTCA
    GCTCTACAAT CATCAAACAA CCTCTTGAGA GAAGTGAATC TGAGTCGCAA TGAGCTGCAG
    GATTCAGGAG TGAAGCTGAT CTGTGCTGGA CTGAAGAGCC CAAACTGTCA ACTCAACGTT
    CTGAAATTGT CAGGCTGTAA TCTCACTAAA CAGTCCTGTG AAACTGTGGC TGTGTCTCTA
    CTTTCATCAA ACTGCCGCCT GAGAGAGCTG GACCTGAGTC GCAATGACCT GCAGGATTCA
    GGAGTGAAGC TGATCTGTGC TGGACTGAAA AGTGCAAACT GTCAACTCAA CACACTGAGA
    CTGGCAAAAT GTAACCTCAC TGATCAGTGC TGCGAAACTA TATCTTCATT TCTACAATCA
    TCAAACTCTG TCTTGAGAGA GCTGGACATA AGCAACAATC GTCTGCAGGA TTCAGGAGTG
    AAGCTGATTT TTACTGCAAT AAAGAGTGAG AACTGTCAAC TCAACATCCT GAGATTGTCA
    AGTTGTAACC TCACTGATCA GTGCTGTGAA ACTTTGGCTT CATCTCTACA ATCATCAAAC
    TGTGTCCTGA GAGAGCTGGA CCTGAGTAAC AATGAGCTGC AGGATTCAGG AGTGAAGCTG
    ATCTGTGCTG GATTGAAGAG CCCAAACTGT CAACTCAACA CACTGAGATT GTCAGGCTGT
    AATCTCACTG ATCAGTGCTG TGAAACTGTG GCTTCATCTC TACAATTATC CAACTGCCCT
    CTGAGAGAGC TGGACCTGAG TCACAATGAT CTTCAGGGTT CAAGAGTGAA GCTGATCTGT
    GCTGGACTGA AGAGCCCAAA CTGTCAACTC AACACACTGA GACTGGCAAA ATGCAACCTC
    ACTGATGAGT GCTGTGAAAC TGTGGCTTCA TCTCTACAAT CATTAAACTG TGTCCTGAGA
    GAGCTGGACC TAAACAGCAA TTCTCTGCAG GATTCAGGAG TGAAGCTGAT TTTTACCGCA
    ATAAAGAGTG AGAACTGCCA GCTCAACACA CTGAGAATGT CAGACTGTAA TCTCACTGAT
    CAGTGCTGTG AAACTGTGGC TTCAGCTCTA CAATCATCAA ACTGCCCCCT GAGTGAGCTG
    GACCTGAGTA ATAATGAGCT GCAGGATTCA GGAGTGAAGC TGATCTGTGC TGGATTAAAG
    AGCCCAAACT GTCAACTCAA TATACTGAGA TTATCTTACT GTTTGGTGTC AGAGGAAGGT
    TGTGCAGCTC TGGCTTCAGC TCTGAGCTCA AACCCGTCAC ACCTGAGAGA GCTGGATCTG
    AGCTACAATC ACCCAGGAGA AGCAGGACTC AAACTGCTCT CTGATCAGCT CAAACACCTC
    CACAAACTCA ATGTGGATCA TGGAGGAGAA TTCAGGACTG TATCAACACT ACACAAATAC
    GCCTGTGAAC TCACACTGGA TCCAAACACA GCACACACAC GCCTCGCTCT GTCTGAGGAG
    AACAGAAAGG CGACGCGTGT GAAGGAGAGT CAGTCTTATC CTCGTCATCC AGACAGATTT
    GATAAGTTTC ATCAGGTTAT GAGTAAAGAG AGTCTGACTG GACGCTGTTA CTGGGAGACT
    CAATGGAGCG GATATGCTGA AATATCAGTT TGTTATAAAG GAATCAAGAG GAAAGGACGG
    AGTGACTGTC GGTTTGGATG TAATGATCAG TCCTGGAGAC TTGATTGCTC TGATCACTCA
    TTCTCTGTCT GTCACAATAA TGAACTCACA CACATCTCAG CTCGTCCAGA CTCCAGCAGG
    ACAGTAGGAG TGTTTGTGGA CGAGTCGAGC GGCTCTCTGT CCTTCTACAG TGTCTCTGAT
    AAACTCACAC ACTTACACAC ATTCAACACC ACATTCACCG ACACACTCCA CGCTGGATTT
    AGACTTGGTT ATAAATCTTC AGTGTCTCTG TGTCGCATTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.