NHS cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	NHS
Entrez Gene ID	491757
Full Name	NHS actin remodeling regulator
General protein information	Preferred Names NHS actin remodeling regulator Names Nance-Horan syndrome protein
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005641156.3	XP_005641213.1	Nance-Horan syndrome protein isoform X4
XM_014111688.1	XP_013967163.1	Nance-Horan syndrome protein isoform X1
XM_548877.4	XP_548877.2	Nance-Horan syndrome protein isoform X2
XM_014111688.2	XP_013967163.1	Nance-Horan syndrome protein isoform X1
XM_005641156.2	XP_005641213.1	Nance-Horan syndrome protein isoform X3
XM_548877.5	XP_548877.2	Nance-Horan syndrome protein isoform X2
XM_022416354.1	XP_022272062.1	Nance-Horan syndrome protein isoform X3

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NHS	XP_548877.2
Bos taurus (cattle)	NHS	NP_001192657.1
Mus musculus (house mouse)	Nhs	NP_001074521.1
	NHS	XP_001104229.2
Rattus norvegicus (Norway rat)	Nhs	NP_001178662.1
Danio rerio (zebrafish)	nhs	XP_005169435.1
Gallus gallus (chicken)	NHS	XP_416815.4
Danio rerio (zebrafish)	nhsa	XP_001919340.4
Xenopus tropicalis (tropical clawed frog)	LOC100498628	XP_002934404.2
Homo sapiens (human)	NHS	NP_938011.1
Pan troglodytes (chimpanzee)	NHS	XP_520958.3

Gene Ontology

The following NHS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NHS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe32123	XM_005641156.3 Latest version!	Canis lupus familiaris NHS actin remodeling regulator (NHS), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe285466	XM_022416354.1 Latest version!	Canis lupus familiaris NHS actin remodeling regulator (NHS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe32122	XM_548877.5 Latest version!	Canis lupus familiaris NHS actin remodeling regulator (NHS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe45625	XM_014111688.2 Latest version!	Canis lupus familiaris NHS actin remodeling regulator (NHS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OCe45625	XM_014111688.1	Canis lupus familiaris Nance-Horan syndrome (congenital cataracts and dental anomalies) (NHS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
OCe32123	XM_005641156.2	Canis lupus familiaris Nance-Horan syndrome (congenital cataracts and dental anomalies) (NHS), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OCe32122	XM_548877.4	Canis lupus familiaris Nance-Horan syndrome (congenital cataracts and dental anomalies) (NHS), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NHS ( XM_005641156.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641213.1 Canis lupus familiaris NHS actin remodeling regulator (NHS), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe32123
Clone ID Related Accession (Same CDS sequence)	XM_005641156.3 , XM_005641156.2
Accession Version	XM_005641156.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Nance-Horan syndrome protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641156.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGGGC AGAGGCCGAA AAACCCAGTA CACAATATCC CTTCAACACT GGACAAGCAG 
ACCAACTGGA GCAAAGCACT GCCTCTCCCA ACACCAGAGG AGAAAATGAA ACAAGATGCC 
CAAGTGATTT CTTCTTGCAT TATTCCCATC AATGTCACTG GAGTTGGTTT TGACAGAGAG 
GCTAGTATAC GCTGCTCTCT TGTTCATTCA CAATCCGTAC TACAGCGGAG ACGAAAACTG 
AGGAGGAGGA AAACCATCTC GGGTATCCCC AGAAGAGTCC AACAAGAAAT AGATTCTGAT 
GAATCCCCTG TGGCCAGGGA AAGGAATGTG ATTGTGCACA CAAATCCAGA CCCTTCCAAC 
ACTGTCAATA GGAGGTCTGG AACCAGGGAC TCAGAGTGCC AAACCGAAGA TATCCTGATT 
GCTGCTCCAT CCAGAAGGAG AATCAGAGCT CAAAGGGGTC AAAGCATAGC AGCTTCCCTT 
TCTCATTCTG CTGGCAACAT CTCTGCATTG GCTGACAAAG GTGACACCAT GTTTACAACT 
GCAGTGAGCA GCCGTACAAG ATCTCGGAGC CTTCCCCGGG AGGGCAACAG AAGTGGGGAT 
GCTGAGCCCA AAGTTGGTGT TAAACCCGCA GCATATGAAG AGGGGGAGCC TTTCATGGGC 
GACCAAGAAA GAACCCCTAA TGATTGCAGT GAGGCCCCCA GCAGCCCAAG TGCACAGGAA 
CACCAGCCTG CTTTGGGTCT GGCTTGTTCT CAACATCTTC ACAGCCCCCA GCACAAGTTA 
AATGAGAGAG GGAGGTCACG TCTGTCCCGA ATGGCTGCTG ACTCTGGCAG TTGTGACATC 
TCCTCCAACT CAGACACCTT TGGGAGCCCC ATCCACTGCA TCTCCACGGC AGGTGTCCTC 
CTCAGCAGCC ACATGGACCA GAAAGATGAC CATCAGTCAT CCAGTGGCAA CTGGAGTGGG 
AGCAGCTCCA CATGCCCCTC ACAGACCTCA GAGACAATCC CTCCTGCAGC TTCTCCTCCC 
CTCACTGGCT CTTCACACTG TGACTCAGAG TTGTCACTAA ACACAGCCCC TCATGCTAAT 
GAGGATGCCA ATGTCTTCGT GACAGAGCAG TACAATGACC ACTTGGATAA AGTGAGAGGC 
CACCGGGCAA ACTCTTTTAC CTCCACTGTG GCGGATCTGC TGGATGACCC CAACAATAGC 
AACACCAGTG ACAGTGAGTG GAATTACCTA CACCACCATC ACGATGCCTC CTGCCGCCAG 
GATTTTAGTC CTGAGCGCCC TAAGGCAGAC AGCCTGGGCT GCCCGAGCTT CACAAGCATG 
GCCACTTACG ACAGCTTTCT GGAAAAGTCT CCATCAGACA AAGCAGACAC TAGCTCTCAC 
TTTTCAGTGG ACACAGAAGG ATACTACACT TCCATGCACT TTGACTGTGG TCTTAAAGGT 
AATAAGAGCT ATGTGTGTCA CTATGCAGCC CTGGGCCCAG AGAATAGCCA GGGCATTGGG 
GTTCCTCCGA CTCTTCCGGA CTGTACCTGG CAGGACTACT TAGACCACAG GAGGCAGGGG 
AGACCAAGCA TCTCTTTCAG GAAACCAAAG GCAAAGCCGA CCCCACCTAA ACGTAGCTCA 
TCTTTGAGGA AGTCCGATGG AAATGCAGAC ATTTCTGAGA AGAAAGAACC AAAGATAAGC 
AGTGGCCAGC TCCTGCCTCA CAGTTCCAGG GAAATGAAGC TGCCTCTTGA TTTCTCCAAC 
ACGCCTTCTC TAATGGAAAA TGCCAACCTT GCCATCAAGC AAGAGTCTTC TTGGATGAAC 
CAGAGTGAAC ATGGTATCAA GGAACCTCAG TTAGACACTT CAGATATTCC GCCATTCAAA 
GATGAAGGTG CTGAATCGAC TCACTATGCA GATCTCTGGC TTCTAAATGA CTTGAAAACA 
AATGATCCTT ACAGATCTTT ATCCAATTCA AGCACTGCTA CGGGTACCAC AGTTATTGAA 
TGCATCAAGT CTCCAGAGAG CTCTGAATCC CAAACATCCC AGTCAGAATC AAGAGCCACC 
ACCCCATCCC TTCCTTCTGT TGACAATGAG TTTAAACTGG CTTCACCAGA AAAGCTGGCA 
GGCTTGGCAT CTCCATCAAG TGGCTACTCG AGCCAGTCTG AAACACCGAC CTCCTCTTTC 
CCTACAGCTT TCTTTTCTGG CCCACTGTCT CCTGGAGGTA GCAAAAGAAA ACCGAAAGTT 
CCAGAAAGAA AATCCTCACT ACAGCAGCCC TCTTTAAAAG ATGGAGCTCT ATCACTGAGT 
AAAGACCTTG AACTTCCAAT TATACCTCCT ACCCATCTTG ACCTAAGTGC TCTTCATAGT 
GTCTTGAATA AACCATTCCA TCACCGTCAC CCATTGCATG TTTTTACTCA TAGTAAACAG 
AACCCAGTAG GAGAAACACT GCGGTCAAAT CCTCCACCAT CCCTTGCAAT CACACCAACG 
GTCCTGAAAT CTGTTAACCT GAGGTCCATC AGTAAATCTG AAGAAGTTAA GCCAAAAGAG 
GGCAACAATA CAGATCTCCC CTACTTGGAG GACAACACTC TCACAATGGC TGCCCTGTCT 
CCAGGTAAGA TTAAGCCACA CATGGCTAAG AAATCAGTAT CACGTCAGTA TTCCACTGAA 
GACACCATAC TGTCCTTTTT AGACTCCTCT GCAGTTGAGA TGGGACCAGA TAAACTACAG 
TTAGAGAAAA AACGTACTTT TGATGTAAAG AATCATTGTG ACCCAGAAAC AGCAACAACC 
TCAGCTGGTA GCAATCTTCT AGATTCAAGT GTGACAAAAG ACCAAATACA AATGGAAAGT 
GAGCCTATTC CAGAAAACAC ACCAAGTAAA AATTGTGACT TTCCCACAGA AGGATTTCAG 
ATGGTCTCTG TTGCCCACCC AAATGATTTG GATGGTAAAG TATTACAATA TGGAGGTGGT 
CCAGATGGAG CCGTTGCACA ACTGCAGAAG CCATCCTCTG CAGATTGGGA GGGAGCCGCA 
CACCCTGAGT CTGTGGATGT GCTCACAGCT CAGTCAAACT CGCCAACCAG ACCCACAGAC 
ATAAGCAGTC AACTTAAGCA TCAACTTGGG ATGAGCCGCC ACCATGACAA AGTGCCTGGG 
AATATCAGCT ATGAAGCAGA GCTATCAAGT GCAAATTCAT GCCCTGAAAA ATGTTCCGAG 
CAAGAAAATA TCGCTTCAGG TATTTCAGCC CAAAGTGCCT CTGATAACAG CGGAGCAGAG 
GAGACCCAAG GAAGTGTGGA CGAGGTTTCA CTGAAAGAAT CGTCACCGAG TGATGACTCT 
ATGATTTCGC CACTTAGTGA AGACTCTCAG GCTGAAGCAG AAGGTGTGTT TGTGTCTCCA 
AACAAACCTC GTACAACTGA AGATTTGTTT GCAGTCATTC ACAGGTCGAA GAGGAAAGTA 
CTTGGAAGAA AAGATTCTGG GGATATGTCT GCTCGAAGCA AATCAAGAGC TTCTCTTGGC 
AGCAGTAGCA GCAGCAACGC TGGCTCTGTC ACTTTGCCCA ACAGCAGCGT GACCAGCCCA 
AACAGCCAGA GGTCTCCTGG CCTCATCTAC CGAAATGCCA AAAAGTCCAA CACATCGAAT 
GAAGAGTTTA AGCTGTTACT CCTCAAGAAA GGAAGTCGCT CAGATTCTAG TTACCGCATG 
TCTGCTACTG AGATCCTGAA AAGTCCCATC CTGCCCAAAC CTCCTGGAGA GCTCACAGCT 
GAATCCCCAC AAAGCACCCA GGAGGCCCAT CAGGGGGCAC CTGGGGCCGA GGCTTTGTCC 
CCACTCTCTC CATGCTCCCC AAGAGTTAAT GCAGAAGGGT TTTCCTCAAA GAACTTTGCC 
ACCTCAGCAG CAGCAAGGGT GGGACGCTCC CGAGCTCCCC CTGCGGCCAG CAGCAGCCGC 
TACAGCGTCC GCTGCAGGCT GTACAATGCA CCCATGCAGG CCATCTCTGA GGGTGAGACA 
GAAAATTCTG ATGGAAGCCC ACATGATGAC CGATCCTCCC AGAGCTCAAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641213.1
CDS	792..4805
Translation	MLGQRPKNPVHNIPSTLDKQTNWSKALPLPTPEEKMKQDAQVISSCIIPINVTGVGFDREASIRCSLVHSQSVLQRRRKLRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPDPSNTVNRRSGTRDSECQTEDILIAAPSRRRIRAQRGQSIAASLSHSAGNISALADKGDTMFTTAVSSRTRSRSLPREGNRSGDAEPKVGVKPAAYEEGEPFMGDQERTPNDCSEAPSSPSAQEHQPALGLACSQHLHSPQHKLNERGRSRLSRMAADSGSCDISSNSDTFGSPIHCISTAGVLLSSHMDQKDDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNTAPHANEDANVFVTEQYNDHLDKVRGHRANSFTSTVADLLDDPNNSNTSDSEWNYLHHHHDASCRQDFSPERPKADSLGCPSFTSMATYDSFLEKSPSDKADTSSHFSVDTEGYYTSMHFDCGLKGNKSYVCHYAALGPENSQGIGVPPTLPDCTWQDYLDHRRQGRPSISFRKPKAKPTPPKRSSSLRKSDGNADISEKKEPKISSGQLLPHSSREMKLPLDFSNTPSLMENANLAIKQESSWMNQSEHGIKEPQLDTSDIPPFKDEGAESTHYADLWLLNDLKTNDPYRSLSNSSTATGTTVIECIKSPESSESQTSQSESRATTPSLPSVDNEFKLASPEKLAGLASPSSGYSSQSETPTSSFPTAFFSGPLSPGGSKRKPKVPERKSSLQQPSLKDGALSLSKDLELPIIPPTHLDLSALHSVLNKPFHHRHPLHVFTHSKQNPVGETLRSNPPPSLAITPTVLKSVNLRSISKSEEVKPKEGNNTDLPYLEDNTLTMAALSPGKIKPHMAKKSVSRQYSTEDTILSFLDSSAVEMGPDKLQLEKKRTFDVKNHCDPETATTSAGSNLLDSSVTKDQIQMESEPIPENTPSKNCDFPTEGFQMVSVAHPNDLDGKVLQYGGGPDGAVAQLQKPSSADWEGAAHPESVDVLTAQSNSPTRPTDISSQLKHQLGMSRHHDKVPGNISYEAELSSANSCPEKCSEQENIASGISAQSASDNSGAEETQGSVDEVSLKESSPSDDSMISPLSEDSQAEAEGVFVSPNKPRTTEDLFAVIHRSKRKVLGRKDSGDMSARSKSRASLGSSSSSNAGSVTLPNSSVTSPNSQRSPGLIYRNAKKSNTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPILPKPPGELTAESPQSTQEAHQGAPGAEALSPLSPCSPRVNAEGFSSKNFATSAAARVGRSRAPPAASSSRYSVRCRLYNAPMQAISEGETENSDGSPHDDRSSQSST

Target ORF information:

RefSeq Version	XM_005641156.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NHS actin remodeling regulator (NHS), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641156.3

ORF Insert Sequence:

ATGTTAGGGC AGAGGCCGAA AAACCCAGTA CACAATATCC CTTCAACACT GGACAAGCAG 
ACCAACTGGA GCAAAGCACT GCCTCTCCCA ACACCAGAGG AGAAAATGAA ACAAGATGCC 
CAAGTGATTT CTTCTTGCAT TATTCCCATC AATGTCACTG GAGTTGGTTT TGACAGAGAG 
GCTAGTATAC GCTGCTCTCT TGTTCATTCA CAATCCGTAC TACAGCGGAG ACGAAAACTG 
AGGAGGAGGA AAACCATCTC GGGTATCCCC AGAAGAGTCC AACAAGAAAT AGATTCTGAT 
GAATCCCCTG TGGCCAGGGA AAGGAATGTG ATTGTGCACA CAAATCCAGA CCCTTCCAAC 
ACTGTCAATA GGAGGTCTGG AACCAGGGAC TCAGAGTGCC AAACCGAAGA TATCCTGATT 
GCTGCTCCAT CCAGAAGGAG AATCAGAGCT CAAAGGGGTC AAAGCATAGC AGCTTCCCTT 
TCTCATTCTG CTGGCAACAT CTCTGCATTG GCTGACAAAG GTGACACCAT GTTTACAACT 
GCAGTGAGCA GCCGTACAAG ATCTCGGAGC CTTCCCCGGG AGGGCAACAG AAGTGGGGAT 
GCTGAGCCCA AAGTTGGTGT TAAACCCGCA GCATATGAAG AGGGGGAGCC TTTCATGGGC 
GACCAAGAAA GAACCCCTAA TGATTGCAGT GAGGCCCCCA GCAGCCCAAG TGCACAGGAA 
CACCAGCCTG CTTTGGGTCT GGCTTGTTCT CAACATCTTC ACAGCCCCCA GCACAAGTTA 
AATGAGAGAG GGAGGTCACG TCTGTCCCGA ATGGCTGCTG ACTCTGGCAG TTGTGACATC 
TCCTCCAACT CAGACACCTT TGGGAGCCCC ATCCACTGCA TCTCCACGGC AGGTGTCCTC 
CTCAGCAGCC ACATGGACCA GAAAGATGAC CATCAGTCAT CCAGTGGCAA CTGGAGTGGG 
AGCAGCTCCA CATGCCCCTC ACAGACCTCA GAGACAATCC CTCCTGCAGC TTCTCCTCCC 
CTCACTGGCT CTTCACACTG TGACTCAGAG TTGTCACTAA ACACAGCCCC TCATGCTAAT 
GAGGATGCCA ATGTCTTCGT GACAGAGCAG TACAATGACC ACTTGGATAA AGTGAGAGGC 
CACCGGGCAA ACTCTTTTAC CTCCACTGTG GCGGATCTGC TGGATGACCC CAACAATAGC 
AACACCAGTG ACAGTGAGTG GAATTACCTA CACCACCATC ACGATGCCTC CTGCCGCCAG 
GATTTTAGTC CTGAGCGCCC TAAGGCAGAC AGCCTGGGCT GCCCGAGCTT CACAAGCATG 
GCCACTTACG ACAGCTTTCT GGAAAAGTCT CCATCAGACA AAGCAGACAC TAGCTCTCAC 
TTTTCAGTGG ACACAGAAGG ATACTACACT TCCATGCACT TTGACTGTGG TCTTAAAGGT 
AATAAGAGCT ATGTGTGTCA CTATGCAGCC CTGGGCCCAG AGAATAGCCA GGGCATTGGG 
GTTCCTCCGA CTCTTCCGGA CTGTACCTGG CAGGACTACT TAGACCACAG GAGGCAGGGG 
AGACCAAGCA TCTCTTTCAG GAAACCAAAG GCAAAGCCGA CCCCACCTAA ACGTAGCTCA 
TCTTTGAGGA AGTCCGATGG AAATGCAGAC ATTTCTGAGA AGAAAGAACC AAAGATAAGC 
AGTGGCCAGC TCCTGCCTCA CAGTTCCAGG GAAATGAAGC TGCCTCTTGA TTTCTCCAAC 
ACGCCTTCTC TAATGGAAAA TGCCAACCTT GCCATCAAGC AAGAGTCTTC TTGGATGAAC 
CAGAGTGAAC ATGGTATCAA GGAACCTCAG TTAGACACTT CAGATATTCC GCCATTCAAA 
GATGAAGGTG CTGAATCGAC TCACTATGCA GATCTCTGGC TTCTAAATGA CTTGAAAACA 
AATGATCCTT ACAGATCTTT ATCCAATTCA AGCACTGCTA CGGGTACCAC AGTTATTGAA 
TGCATCAAGT CTCCAGAGAG CTCTGAATCC CAAACATCCC AGTCAGAATC AAGAGCCACC 
ACCCCATCCC TTCCTTCTGT TGACAATGAG TTTAAACTGG CTTCACCAGA AAAGCTGGCA 
GGCTTGGCAT CTCCATCAAG TGGCTACTCG AGCCAGTCTG AAACACCGAC CTCCTCTTTC 
CCTACAGCTT TCTTTTCTGG CCCACTGTCT CCTGGAGGTA GCAAAAGAAA ACCGAAAGTT 
CCAGAAAGAA AATCCTCACT ACAGCAGCCC TCTTTAAAAG ATGGAGCTCT ATCACTGAGT 
AAAGACCTTG AACTTCCAAT TATACCTCCT ACCCATCTTG ACCTAAGTGC TCTTCATAGT 
GTCTTGAATA AACCATTCCA TCACCGTCAC CCATTGCATG TTTTTACTCA TAGTAAACAG 
AACCCAGTAG GAGAAACACT GCGGTCAAAT CCTCCACCAT CCCTTGCAAT CACACCAACG 
GTCCTGAAAT CTGTTAACCT GAGGTCCATC AGTAAATCTG AAGAAGTTAA GCCAAAAGAG 
GGCAACAATA CAGATCTCCC CTACTTGGAG GACAACACTC TCACAATGGC TGCCCTGTCT 
CCAGGTAAGA TTAAGCCACA CATGGCTAAG AAATCAGTAT CACGTCAGTA TTCCACTGAA 
GACACCATAC TGTCCTTTTT AGACTCCTCT GCAGTTGAGA TGGGACCAGA TAAACTACAG 
TTAGAGAAAA AACGTACTTT TGATGTAAAG AATCATTGTG ACCCAGAAAC AGCAACAACC 
TCAGCTGGTA GCAATCTTCT AGATTCAAGT GTGACAAAAG ACCAAATACA AATGGAAAGT 
GAGCCTATTC CAGAAAACAC ACCAAGTAAA AATTGTGACT TTCCCACAGA AGGATTTCAG 
ATGGTCTCTG TTGCCCACCC AAATGATTTG GATGGTAAAG TATTACAATA TGGAGGTGGT 
CCAGATGGAG CCGTTGCACA ACTGCAGAAG CCATCCTCTG CAGATTGGGA GGGAGCCGCA 
CACCCTGAGT CTGTGGATGT GCTCACAGCT CAGTCAAACT CGCCAACCAG ACCCACAGAC 
ATAAGCAGTC AACTTAAGCA TCAACTTGGG ATGAGCCGCC ACCATGACAA AGTGCCTGGG 
AATATCAGCT ATGAAGCAGA GCTATCAAGT GCAAATTCAT GCCCTGAAAA ATGTTCCGAG 
CAAGAAAATA TCGCTTCAGG TATTTCAGCC CAAAGTGCCT CTGATAACAG CGGAGCAGAG 
GAGACCCAAG GAAGTGTGGA CGAGGTTTCA CTGAAAGAAT CGTCACCGAG TGATGACTCT 
ATGATTTCGC CACTTAGTGA AGACTCTCAG GCTGAAGCAG AAGGTGTGTT TGTGTCTCCA 
AACAAACCTC GTACAACTGA AGATTTGTTT GCAGTCATTC ACAGGTCGAA GAGGAAAGTA 
CTTGGAAGAA AAGATTCTGG GGATATGTCT GCTCGAAGCA AATCAAGAGC TTCTCTTGGC 
AGCAGTAGCA GCAGCAACGC TGGCTCTGTC ACTTTGCCCA ACAGCAGCGT GACCAGCCCA 
AACAGCCAGA GGTCTCCTGG CCTCATCTAC CGAAATGCCA AAAAGTCCAA CACATCGAAT 
GAAGAGTTTA AGCTGTTACT CCTCAAGAAA GGAAGTCGCT CAGATTCTAG TTACCGCATG 
TCTGCTACTG AGATCCTGAA AAGTCCCATC CTGCCCAAAC CTCCTGGAGA GCTCACAGCT 
GAATCCCCAC AAAGCACCCA GGAGGCCCAT CAGGGGGCAC CTGGGGCCGA GGCTTTGTCC 
CCACTCTCTC CATGCTCCCC AAGAGTTAAT GCAGAAGGGT TTTCCTCAAA GAACTTTGCC 
ACCTCAGCAG CAGCAAGGGT GGGACGCTCC CGAGCTCCCC CTGCGGCCAG CAGCAGCCGC 
TACAGCGTCC GCTGCAGGCT GTACAATGCA CCCATGCAGG CCATCTCTGA GGGTGAGACA 
GAAAATTCTG ATGGAAGCCC ACATGATGAC CGATCCTCCC AGAGCTCAAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHS ( XM_005641156.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005641213.1 Canis lupus familiaris Nance-Horan syndrome (congenital cataracts and dental anomalies) (NHS), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OCe32123
Clone ID Related Accession (Same CDS sequence)	XM_005641156.3 , XM_005641156.2
Accession Version	XM_005641156.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4014bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Nance-Horan syndrome protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641156.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAGGGC AGAGGCCGAA AAACCCAGTA CACAATATCC CTTCAACACT GGACAAGCAG 
ACCAACTGGA GCAAAGCACT GCCTCTCCCA ACACCAGAGG AGAAAATGAA ACAAGATGCC 
CAAGTGATTT CTTCTTGCAT TATTCCCATC AATGTCACTG GAGTTGGTTT TGACAGAGAG 
GCTAGTATAC GCTGCTCTCT TGTTCATTCA CAATCCGTAC TACAGCGGAG ACGAAAACTG 
AGGAGGAGGA AAACCATCTC GGGTATCCCC AGAAGAGTCC AACAAGAAAT AGATTCTGAT 
GAATCCCCTG TGGCCAGGGA AAGGAATGTG ATTGTGCACA CAAATCCAGA CCCTTCCAAC 
ACTGTCAATA GGAGGTCTGG AACCAGGGAC TCAGAGTGCC AAACCGAAGA TATCCTGATT 
GCTGCTCCAT CCAGAAGGAG AATCAGAGCT CAAAGGGGTC AAAGCATAGC AGCTTCCCTT 
TCTCATTCTG CTGGCAACAT CTCTGCATTG GCTGACAAAG GTGACACCAT GTTTACAACT 
GCAGTGAGCA GCCGTACAAG ATCTCGGAGC CTTCCCCGGG AGGGCAACAG AAGTGGGGAT 
GCTGAGCCCA AAGTTGGTGT TAAACCCGCA GCATATGAAG AGGGGGAGCC TTTCATGGGC 
GACCAAGAAA GAACCCCTAA TGATTGCAGT GAGGCCCCCA GCAGCCCAAG TGCACAGGAA 
CACCAGCCTG CTTTGGGTCT GGCTTGTTCT CAACATCTTC ACAGCCCCCA GCACAAGTTA 
AATGAGAGAG GGAGGTCACG TCTGTCCCGA ATGGCTGCTG ACTCTGGCAG TTGTGACATC 
TCCTCCAACT CAGACACCTT TGGGAGCCCC ATCCACTGCA TCTCCACGGC AGGTGTCCTC 
CTCAGCAGCC ACATGGACCA GAAAGATGAC CATCAGTCAT CCAGTGGCAA CTGGAGTGGG 
AGCAGCTCCA CATGCCCCTC ACAGACCTCA GAGACAATCC CTCCTGCAGC TTCTCCTCCC 
CTCACTGGCT CTTCACACTG TGACTCAGAG TTGTCACTAA ACACAGCCCC TCATGCTAAT 
GAGGATGCCA ATGTCTTCGT GACAGAGCAG TACAATGACC ACTTGGATAA AGTGAGAGGC 
CACCGGGCAA ACTCTTTTAC CTCCACTGTG GCGGATCTGC TGGATGACCC CAACAATAGC 
AACACCAGTG ACAGTGAGTG GAATTACCTA CACCACCATC ACGATGCCTC CTGCCGCCAG 
GATTTTAGTC CTGAGCGCCC TAAGGCAGAC AGCCTGGGCT GCCCGAGCTT CACAAGCATG 
GCCACTTACG ACAGCTTTCT GGAAAAGTCT CCATCAGACA AAGCAGACAC TAGCTCTCAC 
TTTTCAGTGG ACACAGAAGG ATACTACACT TCCATGCACT TTGACTGTGG TCTTAAAGGT 
AATAAGAGCT ATGTGTGTCA CTATGCAGCC CTGGGCCCAG AGAATAGCCA GGGCATTGGG 
GTTCCTCCGA CTCTTCCGGA CTGTACCTGG CAGGACTACT TAGACCACAG GAGGCAGGGG 
AGACCAAGCA TCTCTTTCAG GAAACCAAAG GCAAAGCCGA CCCCACCTAA ACGTAGCTCA 
TCTTTGAGGA AGTCCGATGG AAATGCAGAC ATTTCTGAGA AGAAAGAACC AAAGATAAGC 
AGTGGCCAGC TCCTGCCTCA CAGTTCCAGG GAAATGAAGC TGCCTCTTGA TTTCTCCAAC 
ACGCCTTCTC TAATGGAAAA TGCCAACCTT GCCATCAAGC AAGAGTCTTC TTGGATGAAC 
CAGAGTGAAC ATGGTATCAA GGAACCTCAG TTAGACACTT CAGATATTCC GCCATTCAAA 
GATGAAGGTG CTGAATCGAC TCACTATGCA GATCTCTGGC TTCTAAATGA CTTGAAAACA 
AATGATCCTT ACAGATCTTT ATCCAATTCA AGCACTGCTA CGGGTACCAC AGTTATTGAA 
TGCATCAAGT CTCCAGAGAG CTCTGAATCC CAAACATCCC AGTCAGAATC AAGAGCCACC 
ACCCCATCCC TTCCTTCTGT TGACAATGAG TTTAAACTGG CTTCACCAGA AAAGCTGGCA 
GGCTTGGCAT CTCCATCAAG TGGCTACTCG AGCCAGTCTG AAACACCGAC CTCCTCTTTC 
CCTACAGCTT TCTTTTCTGG CCCACTGTCT CCTGGAGGTA GCAAAAGAAA ACCGAAAGTT 
CCAGAAAGAA AATCCTCACT ACAGCAGCCC TCTTTAAAAG ATGGAGCTCT ATCACTGAGT 
AAAGACCTTG AACTTCCAAT TATACCTCCT ACCCATCTTG ACCTAAGTGC TCTTCATAGT 
GTCTTGAATA AACCATTCCA TCACCGTCAC CCATTGCATG TTTTTACTCA TAGTAAACAG 
AACCCAGTAG GAGAAACACT GCGGTCAAAT CCTCCACCAT CCCTTGCAAT CACACCAACG 
GTCCTGAAAT CTGTTAACCT GAGGTCCATC AGTAAATCTG AAGAAGTTAA GCCAAAAGAG 
GGCAACAATA CAGATCTCCC CTACTTGGAG GACAACACTC TCACAATGGC TGCCCTGTCT 
CCAGGTAAGA TTAAGCCACA CATGGCTAAG AAATCAGTAT CACGTCAGTA TTCCACTGAA 
GACACCATAC TGTCCTTTTT AGACTCCTCT GCAGTTGAGA TGGGACCAGA TAAACTACAG 
TTAGAGAAAA AACGTACTTT TGATGTAAAG AATCATTGTG ACCCAGAAAC AGCAACAACC 
TCAGCTGGTA GCAATCTTCT AGATTCAAGT GTGACAAAAG ACCAAATACA AATGGAAAGT 
GAGCCTATTC CAGAAAACAC ACCAAGTAAA AATTGTGACT TTCCCACAGA AGGATTTCAG 
ATGGTCTCTG TTGCCCACCC AAATGATTTG GATGGTAAAG TATTACAATA TGGAGGTGGT 
CCAGATGGAG CCGTTGCACA ACTGCAGAAG CCATCCTCTG CAGATTGGGA GGGAGCCGCA 
CACCCTGAGT CTGTGGATGT GCTCACAGCT CAGTCAAACT CGCCAACCAG ACCCACAGAC 
ATAAGCAGTC AACTTAAGCA TCAACTTGGG ATGAGCCGCC ACCATGACAA AGTGCCTGGG 
AATATCAGCT ATGAAGCAGA GCTATCAAGT GCAAATTCAT GCCCTGAAAA ATGTTCCGAG 
CAAGAAAATA TCGCTTCAGG TATTTCAGCC CAAAGTGCCT CTGATAACAG CGGAGCAGAG 
GAGACCCAAG GAAGTGTGGA CGAGGTTTCA CTGAAAGAAT CGTCACCGAG TGATGACTCT 
ATGATTTCGC CACTTAGTGA AGACTCTCAG GCTGAAGCAG AAGGTGTGTT TGTGTCTCCA 
AACAAACCTC GTACAACTGA AGATTTGTTT GCAGTCATTC ACAGGTCGAA GAGGAAAGTA 
CTTGGAAGAA AAGATTCTGG GGATATGTCT GCTCGAAGCA AATCAAGAGC TTCTCTTGGC 
AGCAGTAGCA GCAGCAACGC TGGCTCTGTC ACTTTGCCCA ACAGCAGCGT GACCAGCCCA 
AACAGCCAGA GGTCTCCTGG CCTCATCTAC CGAAATGCCA AAAAGTCCAA CACATCGAAT 
GAAGAGTTTA AGCTGTTACT CCTCAAGAAA GGAAGTCGCT CAGATTCTAG TTACCGCATG 
TCTGCTACTG AGATCCTGAA AAGTCCCATC CTGCCCAAAC CTCCTGGAGA GCTCACAGCT 
GAATCCCCAC AAAGCACCCA GGAGGCCCAT CAGGGGGCAC CTGGGGCCGA GGCTTTGTCC 
CCACTCTCTC CATGCTCCCC AAGAGTTAAT GCAGAAGGGT TTTCCTCAAA GAACTTTGCC 
ACCTCAGCAG CAGCAAGGGT GGGACGCTCC CGAGCTCCCC CTGCGGCCAG CAGCAGCCGC 
TACAGCGTCC GCTGCAGGCT GTACAATGCA CCCATGCAGG CCATCTCTGA GGGTGAGACA 
GAAAATTCTG ATGGAAGCCC ACATGATGAC CGATCCTCCC AGAGCTCAAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005641213.1
CDS	444..4457
Translation	MLGQRPKNPVHNIPSTLDKQTNWSKALPLPTPEEKMKQDAQVISSCIIPINVTGVGFDREASIRCSLVHSQSVLQRRRKLRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPDPSNTVNRRSGTRDSECQTEDILIAAPSRRRIRAQRGQSIAASLSHSAGNISALADKGDTMFTTAVSSRTRSRSLPREGNRSGDAEPKVGVKPAAYEEGEPFMGDQERTPNDCSEAPSSPSAQEHQPALGLACSQHLHSPQHKLNERGRSRLSRMAADSGSCDISSNSDTFGSPIHCISTAGVLLSSHMDQKDDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNTAPHANEDANVFVTEQYNDHLDKVRGHRANSFTSTVADLLDDPNNSNTSDSEWNYLHHHHDASCRQDFSPERPKADSLGCPSFTSMATYDSFLEKSPSDKADTSSHFSVDTEGYYTSMHFDCGLKGNKSYVCHYAALGPENSQGIGVPPTLPDCTWQDYLDHRRQGRPSISFRKPKAKPTPPKRSSSLRKSDGNADISEKKEPKISSGQLLPHSSREMKLPLDFSNTPSLMENANLAIKQESSWMNQSEHGIKEPQLDTSDIPPFKDEGAESTHYADLWLLNDLKTNDPYRSLSNSSTATGTTVIECIKSPESSESQTSQSESRATTPSLPSVDNEFKLASPEKLAGLASPSSGYSSQSETPTSSFPTAFFSGPLSPGGSKRKPKVPERKSSLQQPSLKDGALSLSKDLELPIIPPTHLDLSALHSVLNKPFHHRHPLHVFTHSKQNPVGETLRSNPPPSLAITPTVLKSVNLRSISKSEEVKPKEGNNTDLPYLEDNTLTMAALSPGKIKPHMAKKSVSRQYSTEDTILSFLDSSAVEMGPDKLQLEKKRTFDVKNHCDPETATTSAGSNLLDSSVTKDQIQMESEPIPENTPSKNCDFPTEGFQMVSVAHPNDLDGKVLQYGGGPDGAVAQLQKPSSADWEGAAHPESVDVLTAQSNSPTRPTDISSQLKHQLGMSRHHDKVPGNISYEAELSSANSCPEKCSEQENIASGISAQSASDNSGAEETQGSVDEVSLKESSPSDDSMISPLSEDSQAEAEGVFVSPNKPRTTEDLFAVIHRSKRKVLGRKDSGDMSARSKSRASLGSSSSSNAGSVTLPNSSVTSPNSQRSPGLIYRNAKKSNTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPILPKPPGELTAESPQSTQEAHQGAPGAEALSPLSPCSPRVNAEGFSSKNFATSAAARVGRSRAPPAASSSRYSVRCRLYNAPMQAISEGETENSDGSPHDDRSSQSST

Target ORF information:

RefSeq Version	XM_005641156.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Nance-Horan syndrome (congenital cataracts and dental anomalies) (NHS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005641156.2

ORF Insert Sequence:

ATGTTAGGGC AGAGGCCGAA AAACCCAGTA CACAATATCC CTTCAACACT GGACAAGCAG 
ACCAACTGGA GCAAAGCACT GCCTCTCCCA ACACCAGAGG AGAAAATGAA ACAAGATGCC 
CAAGTGATTT CTTCTTGCAT TATTCCCATC AATGTCACTG GAGTTGGTTT TGACAGAGAG 
GCTAGTATAC GCTGCTCTCT TGTTCATTCA CAATCCGTAC TACAGCGGAG ACGAAAACTG 
AGGAGGAGGA AAACCATCTC GGGTATCCCC AGAAGAGTCC AACAAGAAAT AGATTCTGAT 
GAATCCCCTG TGGCCAGGGA AAGGAATGTG ATTGTGCACA CAAATCCAGA CCCTTCCAAC 
ACTGTCAATA GGAGGTCTGG AACCAGGGAC TCAGAGTGCC AAACCGAAGA TATCCTGATT 
GCTGCTCCAT CCAGAAGGAG AATCAGAGCT CAAAGGGGTC AAAGCATAGC AGCTTCCCTT 
TCTCATTCTG CTGGCAACAT CTCTGCATTG GCTGACAAAG GTGACACCAT GTTTACAACT 
GCAGTGAGCA GCCGTACAAG ATCTCGGAGC CTTCCCCGGG AGGGCAACAG AAGTGGGGAT 
GCTGAGCCCA AAGTTGGTGT TAAACCCGCA GCATATGAAG AGGGGGAGCC TTTCATGGGC 
GACCAAGAAA GAACCCCTAA TGATTGCAGT GAGGCCCCCA GCAGCCCAAG TGCACAGGAA 
CACCAGCCTG CTTTGGGTCT GGCTTGTTCT CAACATCTTC ACAGCCCCCA GCACAAGTTA 
AATGAGAGAG GGAGGTCACG TCTGTCCCGA ATGGCTGCTG ACTCTGGCAG TTGTGACATC 
TCCTCCAACT CAGACACCTT TGGGAGCCCC ATCCACTGCA TCTCCACGGC AGGTGTCCTC 
CTCAGCAGCC ACATGGACCA GAAAGATGAC CATCAGTCAT CCAGTGGCAA CTGGAGTGGG 
AGCAGCTCCA CATGCCCCTC ACAGACCTCA GAGACAATCC CTCCTGCAGC TTCTCCTCCC 
CTCACTGGCT CTTCACACTG TGACTCAGAG TTGTCACTAA ACACAGCCCC TCATGCTAAT 
GAGGATGCCA ATGTCTTCGT GACAGAGCAG TACAATGACC ACTTGGATAA AGTGAGAGGC 
CACCGGGCAA ACTCTTTTAC CTCCACTGTG GCGGATCTGC TGGATGACCC CAACAATAGC 
AACACCAGTG ACAGTGAGTG GAATTACCTA CACCACCATC ACGATGCCTC CTGCCGCCAG 
GATTTTAGTC CTGAGCGCCC TAAGGCAGAC AGCCTGGGCT GCCCGAGCTT CACAAGCATG 
GCCACTTACG ACAGCTTTCT GGAAAAGTCT CCATCAGACA AAGCAGACAC TAGCTCTCAC 
TTTTCAGTGG ACACAGAAGG ATACTACACT TCCATGCACT TTGACTGTGG TCTTAAAGGT 
AATAAGAGCT ATGTGTGTCA CTATGCAGCC CTGGGCCCAG AGAATAGCCA GGGCATTGGG 
GTTCCTCCGA CTCTTCCGGA CTGTACCTGG CAGGACTACT TAGACCACAG GAGGCAGGGG 
AGACCAAGCA TCTCTTTCAG GAAACCAAAG GCAAAGCCGA CCCCACCTAA ACGTAGCTCA 
TCTTTGAGGA AGTCCGATGG AAATGCAGAC ATTTCTGAGA AGAAAGAACC AAAGATAAGC 
AGTGGCCAGC TCCTGCCTCA CAGTTCCAGG GAAATGAAGC TGCCTCTTGA TTTCTCCAAC 
ACGCCTTCTC TAATGGAAAA TGCCAACCTT GCCATCAAGC AAGAGTCTTC TTGGATGAAC 
CAGAGTGAAC ATGGTATCAA GGAACCTCAG TTAGACACTT CAGATATTCC GCCATTCAAA 
GATGAAGGTG CTGAATCGAC TCACTATGCA GATCTCTGGC TTCTAAATGA CTTGAAAACA 
AATGATCCTT ACAGATCTTT ATCCAATTCA AGCACTGCTA CGGGTACCAC AGTTATTGAA 
TGCATCAAGT CTCCAGAGAG CTCTGAATCC CAAACATCCC AGTCAGAATC AAGAGCCACC 
ACCCCATCCC TTCCTTCTGT TGACAATGAG TTTAAACTGG CTTCACCAGA AAAGCTGGCA 
GGCTTGGCAT CTCCATCAAG TGGCTACTCG AGCCAGTCTG AAACACCGAC CTCCTCTTTC 
CCTACAGCTT TCTTTTCTGG CCCACTGTCT CCTGGAGGTA GCAAAAGAAA ACCGAAAGTT 
CCAGAAAGAA AATCCTCACT ACAGCAGCCC TCTTTAAAAG ATGGAGCTCT ATCACTGAGT 
AAAGACCTTG AACTTCCAAT TATACCTCCT ACCCATCTTG ACCTAAGTGC TCTTCATAGT 
GTCTTGAATA AACCATTCCA TCACCGTCAC CCATTGCATG TTTTTACTCA TAGTAAACAG 
AACCCAGTAG GAGAAACACT GCGGTCAAAT CCTCCACCAT CCCTTGCAAT CACACCAACG 
GTCCTGAAAT CTGTTAACCT GAGGTCCATC AGTAAATCTG AAGAAGTTAA GCCAAAAGAG 
GGCAACAATA CAGATCTCCC CTACTTGGAG GACAACACTC TCACAATGGC TGCCCTGTCT 
CCAGGTAAGA TTAAGCCACA CATGGCTAAG AAATCAGTAT CACGTCAGTA TTCCACTGAA 
GACACCATAC TGTCCTTTTT AGACTCCTCT GCAGTTGAGA TGGGACCAGA TAAACTACAG 
TTAGAGAAAA AACGTACTTT TGATGTAAAG AATCATTGTG ACCCAGAAAC AGCAACAACC 
TCAGCTGGTA GCAATCTTCT AGATTCAAGT GTGACAAAAG ACCAAATACA AATGGAAAGT 
GAGCCTATTC CAGAAAACAC ACCAAGTAAA AATTGTGACT TTCCCACAGA AGGATTTCAG 
ATGGTCTCTG TTGCCCACCC AAATGATTTG GATGGTAAAG TATTACAATA TGGAGGTGGT 
CCAGATGGAG CCGTTGCACA ACTGCAGAAG CCATCCTCTG CAGATTGGGA GGGAGCCGCA 
CACCCTGAGT CTGTGGATGT GCTCACAGCT CAGTCAAACT CGCCAACCAG ACCCACAGAC 
ATAAGCAGTC AACTTAAGCA TCAACTTGGG ATGAGCCGCC ACCATGACAA AGTGCCTGGG 
AATATCAGCT ATGAAGCAGA GCTATCAAGT GCAAATTCAT GCCCTGAAAA ATGTTCCGAG 
CAAGAAAATA TCGCTTCAGG TATTTCAGCC CAAAGTGCCT CTGATAACAG CGGAGCAGAG 
GAGACCCAAG GAAGTGTGGA CGAGGTTTCA CTGAAAGAAT CGTCACCGAG TGATGACTCT 
ATGATTTCGC CACTTAGTGA AGACTCTCAG GCTGAAGCAG AAGGTGTGTT TGTGTCTCCA 
AACAAACCTC GTACAACTGA AGATTTGTTT GCAGTCATTC ACAGGTCGAA GAGGAAAGTA 
CTTGGAAGAA AAGATTCTGG GGATATGTCT GCTCGAAGCA AATCAAGAGC TTCTCTTGGC 
AGCAGTAGCA GCAGCAACGC TGGCTCTGTC ACTTTGCCCA ACAGCAGCGT GACCAGCCCA 
AACAGCCAGA GGTCTCCTGG CCTCATCTAC CGAAATGCCA AAAAGTCCAA CACATCGAAT 
GAAGAGTTTA AGCTGTTACT CCTCAAGAAA GGAAGTCGCT CAGATTCTAG TTACCGCATG 
TCTGCTACTG AGATCCTGAA AAGTCCCATC CTGCCCAAAC CTCCTGGAGA GCTCACAGCT 
GAATCCCCAC AAAGCACCCA GGAGGCCCAT CAGGGGGCAC CTGGGGCCGA GGCTTTGTCC 
CCACTCTCTC CATGCTCCCC AAGAGTTAAT GCAGAAGGGT TTTCCTCAAA GAACTTTGCC 
ACCTCAGCAG CAGCAAGGGT GGGACGCTCC CGAGCTCCCC CTGCGGCCAG CAGCAGCCGC 
TACAGCGTCC GCTGCAGGCT GTACAATGCA CCCATGCAGG CCATCTCTGA GGGTGAGACA 
GAAAATTCTG ATGGAAGCCC ACATGATGAC CGATCCTCCC AGAGCTCAAC ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHS ( XM_022416354.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022272062.1 Canis lupus familiaris NHS actin remodeling regulator (NHS), transcript variant X3, mRNA.

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CloneID	OCe285466
Clone ID Related Accession (Same CDS sequence)	XM_022416354.1
Accession Version	XM_022416354.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Nance-Horan syndrome protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTGCG AAAATCCGCC ACCTCCCCTC CGAGGGTCGC CCTCTCGCGC CCTCCCGCCC 
CCGCTCGGCC CGCCGGCCCC AGCAGATCGG CTTTCAGCTC CCTGGGACTC CGGGAGGCTT 
CGGATAAAAG AAGCGGCCGG GCCTTACGAA GCAGAGCGGG AATCGAGCAT GCCTCTCGCC 
TGCTGCCTGC CCAAGAATGC AGCTGTATCC AACCTGGACA TTGAGAGTAA GCTGAGTGTA 
TACTACCGTG CACCATGGCA CCAGCAGAGA AACATCTTCC TCCCAGCCAC AAGGCCACCC 
TGCGTGGAGG AACTGCATCG CCATGCCCGG CAGAGCCTGC AAGCCCTGCG CAGAGAACAC 
CGGAGCCGAA GTGATCGCCG GGAGCAGAGA GCCGCTGCCC CCCTTTCTGT AGCAGCTCCT 
CCACTGCCAG CCTATCCACC AGCTCACAGC CAGAGGAGGC GTGAAGTTAA GGACCGGCAC 
TTCTTAACGT TTAACAGCAC CCGTTCGCCC TCCCCCACTG AGTGTTGCCA CATGACCCCA 
TGGAGTAGAA AGTCCCATCC CCCGGAGGAT GAAGATACAG ATGTCATGTT AGGGCAGAGG 
CCGAAAAACC CAGTACACAA TATCCCTTCA ACACTGGACA AGCAGACCAA CTGGAGCAAA 
GCACTGCCTC TCCCAACACC AGAGGAGAAA ATGAAACAAG ATGCCCAAGT GATTTCTTCT 
TGCATTATTC CCATCAATGT CACTGGAGTT GGTTTTGACA GAGAGGCTAG TATACGCTGC 
TCTCTTGTTC ATTCACAATC CGTACTACAG CGGAGACGAA AACTGAGGAG GAGGAAAACC 
ATCTCGGGTA TCCCCAGAAG AGTCCAACAA GAAATAGATT CTGATGAATC CCCTGTGGCC 
AGGGAAAGGA ATGTGATTGT GCACACAAAT CCAGACCCTT CCAACACTGT CAATAGGAGG 
TCTGGAACCA GGGACTCAGA GTGCCAAACC GAAGATATCC TGATTGCTGC TCCATCCAGA 
AGGAGAATCA GAGCTCAAAG GGGTCAAAGC ATAGCAGCTT CCCTTTCTCA TTCTGCTGGC 
AACATCTCTG CATTGGCTGA CAAAGGTGAC ACCATGTTTA CAACTGCAGT GAGCAGCCGT 
ACAAGATCTC GGAGCCTTCC CCGGGAGGGC AACAGAAGTG GGGATGCTGA GCCCAAAGTT 
GGTGTTAAAC CCGCAGCATA TGAAGAGGGG GAGCCTTTCA TGGGCGACCA AGAAAGAACC 
CCTAATGATT GCAGTGAGGC CCCCAGCAGC CCAAGTGCAC AGGAACACCA GCCTGCTTTG 
GGTCTGGCTT GTTCTCAACA TCTTCACAGC CCCCAGCACA AGTTAAATGA GAGAGGGAGG 
TCACGTCTGT CCCGAATGGC TGCTGACTCT GGCAGTTGTG ACATCTCCTC CAACTCAGAC 
ACCTTTGGGA GCCCCATCCA CTGCATCTCC ACGGCAGGTG TCCTCCTCAG CAGCCACATG 
GACCAGAAAG ATGACCATCA GTCATCCAGT GGCAACTGGA GTGGGAGCAG CTCCACATGC 
CCCTCACAGA CCTCAGAGAC AATCCCTCCT GCAGCTTCTC CTCCCCTCAC TGGCTCTTCA 
CACTGTGACT CAGAGTTGTC ACTAAACACA GCCCCTCATG CTAATGAGGA TGCCAATGTC 
TTCGTGACAG AGCAGTACAA TGACCACTTG GATAAAGTGA GAGGCCACCG GGCAAACTCT 
TTTACCTCCA CTGTGGCGGA TCTGCTGGAT GACCCCAACA ATAGCAACAC CAGTGACAGT 
GAGTGGAATT ACCTACACCA CCATCACGAT GCCTCCTGCC GCCAGGATTT TAGTCCTGAG 
CGCCCTAAGG CAGACAGCCT GGGCTGCCCG AGCTTCACAA GCATGGCCAC TTACGACAGC 
TTTCTGGAAA AGTCTCCATC AGACAAAGCA GACACTAGCT CTCACTTTTC AGTGGACACA 
GAAGGATACT ACACTTCCAT GCACTTTGAC TGTGGTCTTA AAGGTAATAA GAGCTATGTG 
TGTCACTATG CAGCCCTGGG CCCAGAGAAT AGCCAGGGCA TTGGGGTTCC TCCGACTCTT 
CCGGACTGTA CCTGGCAGGA CTACTTAGAC CACAGGAGGC AGGGGAGACC AAGCATCTCT 
TTCAGGAAAC CAAAGGCAAA GCCGACCCCA CCTAAACGTA GCTCATCTTT GAGGAAGTCC 
GATGGAAATG CAGACATTTC TGAGAAGAAA GAACCAAAGA TAAGCAGTGG CCAGCTCCTG 
CCTCACAGTT CCAGGGAAAT GAAGCTGCCT CTTGATTTCT CCAACACGCC TTCTCTAATG 
GAAAATGCCA ACCTTGCCAT CAAGCAAGAG TCTTCTTGGA TGAACCAGAG TGAACATGGT 
ATCAAGGAAC CTCAGTTAGA CACTTCAGAT ATTCCGCCAT TCAAAGATGA AGGTGCTGAA 
TCGACTCACT ATGCAGATCT CTGGCTTCTA AATGACTTGA AAACAAATGA TCCTTACAGA 
TCTTTATCCA ATTCAAGCAC TGCTACGGGT ACCACAGTTA TTGAATGCAT CAAGTCTCCA 
GAGAGCTCTG AATCCCAAAC ATCCCAGTCA GAATCAAGAG CCACCACCCC ATCCCTTCCT 
TCTGTTGACA ATGAGTTTAA ACTGGCTTCA CCAGAAAAGC TGGCAGGCTT GGCATCTCCA 
TCAAGTGGCT ACTCGAGCCA GTCTGAAACA CCGACCTCCT CTTTCCCTAC AGCTTTCTTT 
TCTGGCCCAC TGTCTCCTGG AGGTAGCAAA AGAAAACCGA AAGTTCCAGA AAGAAAATCC 
TCACTACAGC AGCCCTCTTT AAAAGATGGA GCTCTATCAC TGAGTAAAGA CCTTGAACTT 
CCAATTATAC CTCCTACCCA TCTTGACCTA AGTGCTCTTC ATAGTGTCTT GAATAAACCA 
TTCCATCACC GTCACCCATT GCATGTTTTT ACTCATAGTA AACAGAACCC AGTAGGAGAA 
ACACTGCGGT CAAATCCTCC ACCATCCCTT GCAATCACAC CAACGGTCCT GAAATCTGTT 
AACCTGAGGT CCATCAGTAA ATCTGAAGAA GTTAAGCCAA AAGAGGGCAA CAATACAGAT 
CTCCCCTACT TGGAGGACAA CACTCTCACA ATGGCTGCCC TGTCTCCAGG TAAGATTAAG 
CCACACATGG CTAAGAAATC AGTATCACGT CAGTATTCCA CTGAAGACAC CATACTGTCC 
TTTTTAGACT CCTCTGCAGT TGAGATGGGA CCAGATAAAC TACAGTTAGA GAAAAAACGT 
ACTTTTGATG TAAAGAATCA TTGTGACCCA GAAACAGCAA CAACCTCAGC TGGTAGCAAT 
CTTCTAGATT CAAGTGTGAC AAAAGACCAA ATACAAATGG AAAGTGAGCC TATTCCAGAA 
AACACACCAA GTAAAAATTG TGACTTTCCC ACAGAAGGAT TTCAGATGGT CTCTGTTGCC 
CACCCAAATG ATTTGGATGG TAAAGTATTA CAATATGGAG GTGGTCCAGA TGGAGCCGTT 
GCACAACTGC AGAAGCCATC CTCTGCAGAT TGGGAGGGAG CCGCACACCC TGAGTCTGTG 
GATGTGCTCA CAGCTCAGTC AAACTCGCCA ACCAGACCCA CAGACATAAG CAGTCAACTT 
AAGCATCAAC TTGGGATGAG CCGCCACCAT GACAAAGTGC CTGGGAATAT CAGCTATGAA 
GCAGAGCTAT CAAGTGCAAA TTCATGCCCT GAAAAATGTT CCGAGCAAGA AAATATCGCT 
TCAGGTATTT CAGCCCAAAG TGCCTCTGAT AACAGCGGAG CAGAGGAGAC CCAAGGAAGT 
GTGGACGAGG TTTCACTGAA AGAATCGTCA CCGAGTGATG ACTCTATGAT TTCGCCACTT 
AGTGAAGACT CTCAGGCTGA AGCAGAAGGT GTGTTTGTGT CTCCAAACAA ACCTCGTACA 
ACTGAAGATT TGTTTGCAGT CATTCACAGG TCGAAGAGGA AAGTACTTGG AAGAAAAGAT 
TCTGGGGATA TGTCTGCTCG AAGCAAATCA AGAGCTTCTC TTGGCAGCAG TAGCAGCAGC 
AACGCTGGCT CTGTCACTTT GCCCAACAGC AGCGTGACCA GCCCAAACAG CCAGAGGTCT 
CCTGGCCTCA TCTACCGAAA TGCCAAAAAG TCCAACACAT CGAATGAAGA GTTTAAGCTG 
TTACTCCTCA AGAAAGGAAG TCGCTCAGAT TCTAGTTACC GCATGTCTGC TACTGAGATC 
CTGAAAAGTC CCATCCTGCC CAAACCTCCT GGAGAGCTCA CAGCTGAATC CCCACAAAGC 
ACCCAGGAGG CCCATCAGGG GGCACCTGGG GCCGAGGCTT TGTCCCCACT CTCTCCATGC 
TCCCCAAGAG TTAATGCAGA AGGGTTTTCC TCAAAGAACT TTGCCACCTC AGCAGCAGCA 
AGGGTGGGAC GCTCCCGAGC TCCCCCTGCG GCCAGCAGCA GCCGCTACAG CGTCCGCTGC 
AGGCTGTACA ATGCACCCAT GCAGGCCATC TCTGAGGGTG AGACAGAAAA TTCTGATGGA 
AGCCCACATG ATGACCGATC CTCCCAGAGC TCAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022272062.1
CDS	18..4616
Translation	MLCENPPPPLRGSPSRALPPPLGPPAPADRLSAPWDSGRLRIKEAAGPYEAERESSMPLACCLPKNAAVSNLDIESKLSVYYRAPWHQQRNIFLPATRPPCVEELHRHARQSLQALRREHRSRSDRREQRAAAPLSVAAPPLPAYPPAHSQRRREVKDRHFLTFNSTRSPSPTECCHMTPWSRKSHPPEDEDTDVMLGQRPKNPVHNIPSTLDKQTNWSKALPLPTPEEKMKQDAQVISSCIIPINVTGVGFDREASIRCSLVHSQSVLQRRRKLRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPDPSNTVNRRSGTRDSECQTEDILIAAPSRRRIRAQRGQSIAASLSHSAGNISALADKGDTMFTTAVSSRTRSRSLPREGNRSGDAEPKVGVKPAAYEEGEPFMGDQERTPNDCSEAPSSPSAQEHQPALGLACSQHLHSPQHKLNERGRSRLSRMAADSGSCDISSNSDTFGSPIHCISTAGVLLSSHMDQKDDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNTAPHANEDANVFVTEQYNDHLDKVRGHRANSFTSTVADLLDDPNNSNTSDSEWNYLHHHHDASCRQDFSPERPKADSLGCPSFTSMATYDSFLEKSPSDKADTSSHFSVDTEGYYTSMHFDCGLKGNKSYVCHYAALGPENSQGIGVPPTLPDCTWQDYLDHRRQGRPSISFRKPKAKPTPPKRSSSLRKSDGNADISEKKEPKISSGQLLPHSSREMKLPLDFSNTPSLMENANLAIKQESSWMNQSEHGIKEPQLDTSDIPPFKDEGAESTHYADLWLLNDLKTNDPYRSLSNSSTATGTTVIECIKSPESSESQTSQSESRATTPSLPSVDNEFKLASPEKLAGLASPSSGYSSQSETPTSSFPTAFFSGPLSPGGSKRKPKVPERKSSLQQPSLKDGALSLSKDLELPIIPPTHLDLSALHSVLNKPFHHRHPLHVFTHSKQNPVGETLRSNPPPSLAITPTVLKSVNLRSISKSEEVKPKEGNNTDLPYLEDNTLTMAALSPGKIKPHMAKKSVSRQYSTEDTILSFLDSSAVEMGPDKLQLEKKRTFDVKNHCDPETATTSAGSNLLDSSVTKDQIQMESEPIPENTPSKNCDFPTEGFQMVSVAHPNDLDGKVLQYGGGPDGAVAQLQKPSSADWEGAAHPESVDVLTAQSNSPTRPTDISSQLKHQLGMSRHHDKVPGNISYEAELSSANSCPEKCSEQENIASGISAQSASDNSGAEETQGSVDEVSLKESSPSDDSMISPLSEDSQAEAEGVFVSPNKPRTTEDLFAVIHRSKRKVLGRKDSGDMSARSKSRASLGSSSSSNAGSVTLPNSSVTSPNSQRSPGLIYRNAKKSNTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPILPKPPGELTAESPQSTQEAHQGAPGAEALSPLSPCSPRVNAEGFSSKNFATSAAARVGRSRAPPAASSSRYSVRCRLYNAPMQAISEGETENSDGSPHDDRSSQSST

Target ORF information:

RefSeq Version	XM_022416354.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NHS actin remodeling regulator (NHS), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022416354.1

ORF Insert Sequence:

ATGCTGTGCG AAAATCCGCC ACCTCCCCTC CGAGGGTCGC CCTCTCGCGC CCTCCCGCCC 
CCGCTCGGCC CGCCGGCCCC AGCAGATCGG CTTTCAGCTC CCTGGGACTC CGGGAGGCTT 
CGGATAAAAG AAGCGGCCGG GCCTTACGAA GCAGAGCGGG AATCGAGCAT GCCTCTCGCC 
TGCTGCCTGC CCAAGAATGC AGCTGTATCC AACCTGGACA TTGAGAGTAA GCTGAGTGTA 
TACTACCGTG CACCATGGCA CCAGCAGAGA AACATCTTCC TCCCAGCCAC AAGGCCACCC 
TGCGTGGAGG AACTGCATCG CCATGCCCGG CAGAGCCTGC AAGCCCTGCG CAGAGAACAC 
CGGAGCCGAA GTGATCGCCG GGAGCAGAGA GCCGCTGCCC CCCTTTCTGT AGCAGCTCCT 
CCACTGCCAG CCTATCCACC AGCTCACAGC CAGAGGAGGC GTGAAGTTAA GGACCGGCAC 
TTCTTAACGT TTAACAGCAC CCGTTCGCCC TCCCCCACTG AGTGTTGCCA CATGACCCCA 
TGGAGTAGAA AGTCCCATCC CCCGGAGGAT GAAGATACAG ATGTCATGTT AGGGCAGAGG 
CCGAAAAACC CAGTACACAA TATCCCTTCA ACACTGGACA AGCAGACCAA CTGGAGCAAA 
GCACTGCCTC TCCCAACACC AGAGGAGAAA ATGAAACAAG ATGCCCAAGT GATTTCTTCT 
TGCATTATTC CCATCAATGT CACTGGAGTT GGTTTTGACA GAGAGGCTAG TATACGCTGC 
TCTCTTGTTC ATTCACAATC CGTACTACAG CGGAGACGAA AACTGAGGAG GAGGAAAACC 
ATCTCGGGTA TCCCCAGAAG AGTCCAACAA GAAATAGATT CTGATGAATC CCCTGTGGCC 
AGGGAAAGGA ATGTGATTGT GCACACAAAT CCAGACCCTT CCAACACTGT CAATAGGAGG 
TCTGGAACCA GGGACTCAGA GTGCCAAACC GAAGATATCC TGATTGCTGC TCCATCCAGA 
AGGAGAATCA GAGCTCAAAG GGGTCAAAGC ATAGCAGCTT CCCTTTCTCA TTCTGCTGGC 
AACATCTCTG CATTGGCTGA CAAAGGTGAC ACCATGTTTA CAACTGCAGT GAGCAGCCGT 
ACAAGATCTC GGAGCCTTCC CCGGGAGGGC AACAGAAGTG GGGATGCTGA GCCCAAAGTT 
GGTGTTAAAC CCGCAGCATA TGAAGAGGGG GAGCCTTTCA TGGGCGACCA AGAAAGAACC 
CCTAATGATT GCAGTGAGGC CCCCAGCAGC CCAAGTGCAC AGGAACACCA GCCTGCTTTG 
GGTCTGGCTT GTTCTCAACA TCTTCACAGC CCCCAGCACA AGTTAAATGA GAGAGGGAGG 
TCACGTCTGT CCCGAATGGC TGCTGACTCT GGCAGTTGTG ACATCTCCTC CAACTCAGAC 
ACCTTTGGGA GCCCCATCCA CTGCATCTCC ACGGCAGGTG TCCTCCTCAG CAGCCACATG 
GACCAGAAAG ATGACCATCA GTCATCCAGT GGCAACTGGA GTGGGAGCAG CTCCACATGC 
CCCTCACAGA CCTCAGAGAC AATCCCTCCT GCAGCTTCTC CTCCCCTCAC TGGCTCTTCA 
CACTGTGACT CAGAGTTGTC ACTAAACACA GCCCCTCATG CTAATGAGGA TGCCAATGTC 
TTCGTGACAG AGCAGTACAA TGACCACTTG GATAAAGTGA GAGGCCACCG GGCAAACTCT 
TTTACCTCCA CTGTGGCGGA TCTGCTGGAT GACCCCAACA ATAGCAACAC CAGTGACAGT 
GAGTGGAATT ACCTACACCA CCATCACGAT GCCTCCTGCC GCCAGGATTT TAGTCCTGAG 
CGCCCTAAGG CAGACAGCCT GGGCTGCCCG AGCTTCACAA GCATGGCCAC TTACGACAGC 
TTTCTGGAAA AGTCTCCATC AGACAAAGCA GACACTAGCT CTCACTTTTC AGTGGACACA 
GAAGGATACT ACACTTCCAT GCACTTTGAC TGTGGTCTTA AAGGTAATAA GAGCTATGTG 
TGTCACTATG CAGCCCTGGG CCCAGAGAAT AGCCAGGGCA TTGGGGTTCC TCCGACTCTT 
CCGGACTGTA CCTGGCAGGA CTACTTAGAC CACAGGAGGC AGGGGAGACC AAGCATCTCT 
TTCAGGAAAC CAAAGGCAAA GCCGACCCCA CCTAAACGTA GCTCATCTTT GAGGAAGTCC 
GATGGAAATG CAGACATTTC TGAGAAGAAA GAACCAAAGA TAAGCAGTGG CCAGCTCCTG 
CCTCACAGTT CCAGGGAAAT GAAGCTGCCT CTTGATTTCT CCAACACGCC TTCTCTAATG 
GAAAATGCCA ACCTTGCCAT CAAGCAAGAG TCTTCTTGGA TGAACCAGAG TGAACATGGT 
ATCAAGGAAC CTCAGTTAGA CACTTCAGAT ATTCCGCCAT TCAAAGATGA AGGTGCTGAA 
TCGACTCACT ATGCAGATCT CTGGCTTCTA AATGACTTGA AAACAAATGA TCCTTACAGA 
TCTTTATCCA ATTCAAGCAC TGCTACGGGT ACCACAGTTA TTGAATGCAT CAAGTCTCCA 
GAGAGCTCTG AATCCCAAAC ATCCCAGTCA GAATCAAGAG CCACCACCCC ATCCCTTCCT 
TCTGTTGACA ATGAGTTTAA ACTGGCTTCA CCAGAAAAGC TGGCAGGCTT GGCATCTCCA 
TCAAGTGGCT ACTCGAGCCA GTCTGAAACA CCGACCTCCT CTTTCCCTAC AGCTTTCTTT 
TCTGGCCCAC TGTCTCCTGG AGGTAGCAAA AGAAAACCGA AAGTTCCAGA AAGAAAATCC 
TCACTACAGC AGCCCTCTTT AAAAGATGGA GCTCTATCAC TGAGTAAAGA CCTTGAACTT 
CCAATTATAC CTCCTACCCA TCTTGACCTA AGTGCTCTTC ATAGTGTCTT GAATAAACCA 
TTCCATCACC GTCACCCATT GCATGTTTTT ACTCATAGTA AACAGAACCC AGTAGGAGAA 
ACACTGCGGT CAAATCCTCC ACCATCCCTT GCAATCACAC CAACGGTCCT GAAATCTGTT 
AACCTGAGGT CCATCAGTAA ATCTGAAGAA GTTAAGCCAA AAGAGGGCAA CAATACAGAT 
CTCCCCTACT TGGAGGACAA CACTCTCACA ATGGCTGCCC TGTCTCCAGG TAAGATTAAG 
CCACACATGG CTAAGAAATC AGTATCACGT CAGTATTCCA CTGAAGACAC CATACTGTCC 
TTTTTAGACT CCTCTGCAGT TGAGATGGGA CCAGATAAAC TACAGTTAGA GAAAAAACGT 
ACTTTTGATG TAAAGAATCA TTGTGACCCA GAAACAGCAA CAACCTCAGC TGGTAGCAAT 
CTTCTAGATT CAAGTGTGAC AAAAGACCAA ATACAAATGG AAAGTGAGCC TATTCCAGAA 
AACACACCAA GTAAAAATTG TGACTTTCCC ACAGAAGGAT TTCAGATGGT CTCTGTTGCC 
CACCCAAATG ATTTGGATGG TAAAGTATTA CAATATGGAG GTGGTCCAGA TGGAGCCGTT 
GCACAACTGC AGAAGCCATC CTCTGCAGAT TGGGAGGGAG CCGCACACCC TGAGTCTGTG 
GATGTGCTCA CAGCTCAGTC AAACTCGCCA ACCAGACCCA CAGACATAAG CAGTCAACTT 
AAGCATCAAC TTGGGATGAG CCGCCACCAT GACAAAGTGC CTGGGAATAT CAGCTATGAA 
GCAGAGCTAT CAAGTGCAAA TTCATGCCCT GAAAAATGTT CCGAGCAAGA AAATATCGCT 
TCAGGTATTT CAGCCCAAAG TGCCTCTGAT AACAGCGGAG CAGAGGAGAC CCAAGGAAGT 
GTGGACGAGG TTTCACTGAA AGAATCGTCA CCGAGTGATG ACTCTATGAT TTCGCCACTT 
AGTGAAGACT CTCAGGCTGA AGCAGAAGGT GTGTTTGTGT CTCCAAACAA ACCTCGTACA 
ACTGAAGATT TGTTTGCAGT CATTCACAGG TCGAAGAGGA AAGTACTTGG AAGAAAAGAT 
TCTGGGGATA TGTCTGCTCG AAGCAAATCA AGAGCTTCTC TTGGCAGCAG TAGCAGCAGC 
AACGCTGGCT CTGTCACTTT GCCCAACAGC AGCGTGACCA GCCCAAACAG CCAGAGGTCT 
CCTGGCCTCA TCTACCGAAA TGCCAAAAAG TCCAACACAT CGAATGAAGA GTTTAAGCTG 
TTACTCCTCA AGAAAGGAAG TCGCTCAGAT TCTAGTTACC GCATGTCTGC TACTGAGATC 
CTGAAAAGTC CCATCCTGCC CAAACCTCCT GGAGAGCTCA CAGCTGAATC CCCACAAAGC 
ACCCAGGAGG CCCATCAGGG GGCACCTGGG GCCGAGGCTT TGTCCCCACT CTCTCCATGC 
TCCCCAAGAG TTAATGCAGA AGGGTTTTCC TCAAAGAACT TTGCCACCTC AGCAGCAGCA 
AGGGTGGGAC GCTCCCGAGC TCCCCCTGCG GCCAGCAGCA GCCGCTACAG CGTCCGCTGC 
AGGCTGTACA ATGCACCCAT GCAGGCCATC TCTGAGGGTG AGACAGAAAA TTCTGATGGA 
AGCCCACATG ATGACCGATC CTCCCAGAGC TCAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHS ( XM_548877.4 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_548877.2 Canis lupus familiaris Nance-Horan syndrome (congenital cataracts and dental anomalies) (NHS), transcript variant X2, mRNA.

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CloneID	OCe32122
Clone ID Related Accession (Same CDS sequence)	XM_548877.4 , XM_548877.5
Accession Version	XM_548877.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Nance-Horan syndrome protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_548877.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTCG CCAAGAGGAT CGTGGAGCCG CAGTGGCTGT GCCGGCAGCG GCGCCCGGCG 
CCCGGCCCGG CGGAGGACGC GAACGGAGGC AGCGCTGACC CGCCGCCGCC CCTGCAGCCG 
CCCGGCCGAC GGGACGAGGC CGTGGCGCCG GGGCCGGAGG ATCCTCCCCG CGCGCCCCCC 
GCGCCTCCCG GGCCGCCGCC GCCGCCGCCG CTGCCCGCGC CGACCGACCA GGCGCAGCCG 
CCGCACGGAG AGGCGCCAGC GGCGGGCGAG GAGAGCGCGG CGGGGGTCGC CGAGGCGGCG 
TCCGCGGCCG GCGAGGCGTC CTCGGCGGCG GCGGCGGCCG TGCTGCTCAT GCTGGACCTG 
TGCGCGGTCA GCAACGCCGC GCTGGCCCGC GTCCTCCGGC AGCTCTCGGA CGTGGCCCGG 
CACGCGTGCA GCCTCTTCCA GGAGCTCGAG AGCGACATCC AGCTGACCCA CCGCCGCGTC 
TGGGCGCTGC AGGGCAAGCT CGGCGGCGTG CAGCGCGTCC TCGGCACGCT GGACCCCAAG 
CAGGAGGCAG TGCCTGTATC CAACCTGGAC ATTGAGAGTA AGCTGAGTGT ATACTACCGT 
GCACCATGGC ACCAGCAGAG AAACATCTTC CTCCCAGCCA CAAGGCCACC CTGCGTGGAG 
GAACTGCATC GCCATGCCCG GCAGAGCCTG CAAGCCCTGC GCAGAGAACA CCGGAGCCGA 
AGTGATCGCC GGGAGCAGAG AGCCGCTGCC CCCCTTTCTG TAGCAGCTCC TCCACTGCCA 
GCCTATCCAC CAGCTCACAG CCAGAGGAGG CGTGAAGTTA AGGACCGGCA CTTCTTAACG 
TCCCATCCCC CGGAGGATGA AGATACAGAT GTCATGTTAG GGCAGAGGCC GAAAAACCCA 
GTACACAATA TCCCTTCAAC ACTGGACAAG CAGACCAACT GGAGCAAAGC ACTGCCTCTC 
CCAACACCAG AGGAGAAAAT GAAACAAGAT GCCCAAGTGA TTTCTTCTTG CATTATTCCC 
ATCAATGTCA CTGGAGTTGG TTTTGACAGA GAGGCTAGTA TACGCTGCTC TCTTGTTCAT 
TCACAATCCG TACTACAGCG GAGACGAAAA CTGAGGAGGA GGAAAACCAT CTCGGGTATC 
CCCAGAAGAG TCCAACAAGA AATAGATTCT GATGAATCCC CTGTGGCCAG GGAAAGGAAT 
GTGATTGTGC ACACAAATCC AGACCCTTCC AACACTGTCA ATAGGAGGTC TGGAACCAGG 
GACTCAGAGT GCCAAACCGA AGATATCCTG ATTGCTGCTC CATCCAGAAG GAGAATCAGA 
GCTCAAAGGG GTCAAAGCAT AGCAGCTTCC CTTTCTCATT CTGCTGGCAA CATCTCTGCA 
TTGGCTGACA AAGGTGACAC CATGTTTACA ACTGCAGTGA GCAGCCGTAC AAGATCTCGG 
AGCCTTCCCC GGGAGGGCAA CAGAAGTGGG GATGCTGAGC CCAAAGTTGG TGTTAAACCC 
GCAGCATATG AAGAGGGGGA GCCTTTCATG GGCGACCAAG AAAGAACCCC TAATGATTGC 
AGTGAGGCCC CCAGCAGCCC AAGTGCACAG GAACACCAGC CTGCTTTGGG TCTGGCTTGT 
TCTCAACATC TTCACAGCCC CCAGCACAAG TTAAATGAGA GAGGGAGGTC ACGTCTGTCC 
CGAATGGCTG CTGACTCTGG CAGTTGTGAC ATCTCCTCCA ACTCAGACAC CTTTGGGAGC 
CCCATCCACT GCATCTCCAC GGCAGGTGTC CTCCTCAGCA GCCACATGGA CCAGAAAGAT 
GACCATCAGT CATCCAGTGG CAACTGGAGT GGGAGCAGCT CCACATGCCC CTCACAGACC 
TCAGAGACAA TCCCTCCTGC AGCTTCTCCT CCCCTCACTG GCTCTTCACA CTGTGACTCA 
GAGTTGTCAC TAAACACAGC CCCTCATGCT AATGAGGATG CCAATGTCTT CGTGACAGAG 
CAGTACAATG ACCACTTGGA TAAAGTGAGA GGCCACCGGG CAAACTCTTT TACCTCCACT 
GTGGCGGATC TGCTGGATGA CCCCAACAAT AGCAACACCA GTGACAGTGA GTGGAATTAC 
CTACACCACC ATCACGATGC CTCCTGCCGC CAGGATTTTA GTCCTGAGCG CCCTAAGGCA 
GACAGCCTGG GCTGCCCGAG CTTCACAAGC ATGGCCACTT ACGACAGCTT TCTGGAAAAG 
TCTCCATCAG ACAAAGCAGA CACTAGCTCT CACTTTTCAG TGGACACAGA AGGATACTAC 
ACTTCCATGC ACTTTGACTG TGGTCTTAAA GGTAATAAGA GCTATGTGTG TCACTATGCA 
GCCCTGGGCC CAGAGAATAG CCAGGGCATT GGGGTTCCTC CGACTCTTCC GGACTGTACC 
TGGCAGGACT ACTTAGACCA CAGGAGGCAG GGGAGACCAA GCATCTCTTT CAGGAAACCA 
AAGGCAAAGC CGACCCCACC TAAACGTAGC TCATCTTTGA GGAAGTCCGA TGGAAATGCA 
GACATTTCTG AGAAGAAAGA ACCAAAGATA AGCAGTGGCC AGCTCCTGCC TCACAGTTCC 
AGGGAAATGA AGCTGCCTCT TGATTTCTCC AACACGCCTT CTCTAATGGA AAATGCCAAC 
CTTGCCATCA AGCAAGAGTC TTCTTGGATG AACCAGAGTG AACATGGTAT CAAGGAACCT 
CAGTTAGACA CTTCAGATAT TCCGCCATTC AAAGATGAAG GTGCTGAATC GACTCACTAT 
GCAGATCTCT GGCTTCTAAA TGACTTGAAA ACAAATGATC CTTACAGATC TTTATCCAAT 
TCAAGCACTG CTACGGGTAC CACAGTTATT GAATGCATCA AGTCTCCAGA GAGCTCTGAA 
TCCCAAACAT CCCAGTCAGA ATCAAGAGCC ACCACCCCAT CCCTTCCTTC TGTTGACAAT 
GAGTTTAAAC TGGCTTCACC AGAAAAGCTG GCAGGCTTGG CATCTCCATC AAGTGGCTAC 
TCGAGCCAGT CTGAAACACC GACCTCCTCT TTCCCTACAG CTTTCTTTTC TGGCCCACTG 
TCTCCTGGAG GTAGCAAAAG AAAACCGAAA GTTCCAGAAA GAAAATCCTC ACTACAGCAG 
CCCTCTTTAA AAGATGGAGC TCTATCACTG AGTAAAGACC TTGAACTTCC AATTATACCT 
CCTACCCATC TTGACCTAAG TGCTCTTCAT AGTGTCTTGA ATAAACCATT CCATCACCGT 
CACCCATTGC ATGTTTTTAC TCATAGTAAA CAGAACCCAG TAGGAGAAAC ACTGCGGTCA 
AATCCTCCAC CATCCCTTGC AATCACACCA ACGGTCCTGA AATCTGTTAA CCTGAGGTCC 
ATCAGTAAAT CTGAAGAAGT TAAGCCAAAA GAGGGCAACA ATACAGATCT CCCCTACTTG 
GAGGACAACA CTCTCACAAT GGCTGCCCTG TCTCCAGGTA AGATTAAGCC ACACATGGCT 
AAGAAATCAG TATCACGTCA GTATTCCACT GAAGACACCA TACTGTCCTT TTTAGACTCC 
TCTGCAGTTG AGATGGGACC AGATAAACTA CAGTTAGAGA AAAAACGTAC TTTTGATGTA 
AAGAATCATT GTGACCCAGA AACAGCAACA ACCTCAGCTG GTAGCAATCT TCTAGATTCA 
AGTGTGACAA AAGACCAAAT ACAAATGGAA AGTGAGCCTA TTCCAGAAAA CACACCAAGT 
AAAAATTGTG ACTTTCCCAC AGAAGGATTT CAGATGGTCT CTGTTGCCCA CCCAAATGAT 
TTGGATGGTA AAGTATTACA ATATGGAGGT GGTCCAGATG GAGCCGTTGC ACAACTGCAG 
AAGCCATCCT CTGCAGATTG GGAGGGAGCC GCACACCCTG AGTCTGTGGA TGTGCTCACA 
GCTCAGTCAA ACTCGCCAAC CAGACCCACA GACATAAGCA GTCAACTTAA GCATCAACTT 
GGGATGAGCC GCCACCATGA CAAAGTGCCT GGGAATATCA GCTATGAAGC AGAGCTATCA 
AGTGCAAATT CATGCCCTGA AAAATGTTCC GAGCAAGAAA ATATCGCTTC AGGTATTTCA 
GCCCAAAGTG CCTCTGATAA CAGCGGAGCA GAGGAGACCC AAGGAAGTGT GGACGAGGTT 
TCACTGAAAG AATCGTCACC GAGTGATGAC TCTATGATTT CGCCACTTAG TGAAGACTCT 
CAGGCTGAAG CAGAAGGTGT GTTTGTGTCT CCAAACAAAC CTCGTACAAC TGAAGATTTG 
TTTGCAGTCA TTCACAGGTC GAAGAGGAAA GTACTTGGAA GAAAAGATTC TGGGGATATG 
TCTGCTCGAA GCAAATCAAG AGCTTCTCTT GGCAGCAGTA GCAGCAGCAA CGCTGGCTCT 
GTCACTTTGC CCAACAGCAG CGTGACCAGC CCAAACAGCC AGAGGTCTCC TGGCCTCATC 
TACCGAAATG CCAAAAAGTC CAACACATCG AATGAAGAGT TTAAGCTGTT ACTCCTCAAG 
AAAGGAAGTC GCTCAGATTC TAGTTACCGC ATGTCTGCTA CTGAGATCCT GAAAAGTCCC 
ATCCTGCCCA AACCTCCTGG AGAGCTCACA GCTGAATCCC CACAAAGCAC CCAGGAGGCC 
CATCAGGGGG CACCTGGGGC CGAGGCTTTG TCCCCACTCT CTCCATGCTC CCCAAGAGTT 
AATGCAGAAG GGTTTTCCTC AAAGAACTTT GCCACCTCAG CAGCAGCAAG GGTGGGACGC 
TCCCGAGCTC CCCCTGCGGC CAGCAGCAGC CGCTACAGCG TCCGCTGCAG GCTGTACAAT 
GCACCCATGC AGGCCATCTC TGAGGGTGAG ACAGAAAATT CTGATGGAAG CCCACATGAT 
GACCGATCCT CCCAGAGCTC AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_548877.2
CDS	361..5247
Translation	MPFAKRIVEPQWLCRQRRPAPGPAEDANGGSADPPPPLQPPGRRDEAVAPGPEDPPRAPPAPPGPPPPPPLPAPTDQAQPPHGEAPAAGEESAAGVAEAASAAGEASSAAAAAVLLMLDLCAVSNAALARVLRQLSDVARHACSLFQELESDIQLTHRRVWALQGKLGGVQRVLGTLDPKQEAVPVSNLDIESKLSVYYRAPWHQQRNIFLPATRPPCVEELHRHARQSLQALRREHRSRSDRREQRAAAPLSVAAPPLPAYPPAHSQRRREVKDRHFLTSHPPEDEDTDVMLGQRPKNPVHNIPSTLDKQTNWSKALPLPTPEEKMKQDAQVISSCIIPINVTGVGFDREASIRCSLVHSQSVLQRRRKLRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPDPSNTVNRRSGTRDSECQTEDILIAAPSRRRIRAQRGQSIAASLSHSAGNISALADKGDTMFTTAVSSRTRSRSLPREGNRSGDAEPKVGVKPAAYEEGEPFMGDQERTPNDCSEAPSSPSAQEHQPALGLACSQHLHSPQHKLNERGRSRLSRMAADSGSCDISSNSDTFGSPIHCISTAGVLLSSHMDQKDDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNTAPHANEDANVFVTEQYNDHLDKVRGHRANSFTSTVADLLDDPNNSNTSDSEWNYLHHHHDASCRQDFSPERPKADSLGCPSFTSMATYDSFLEKSPSDKADTSSHFSVDTEGYYTSMHFDCGLKGNKSYVCHYAALGPENSQGIGVPPTLPDCTWQDYLDHRRQGRPSISFRKPKAKPTPPKRSSSLRKSDGNADISEKKEPKISSGQLLPHSSREMKLPLDFSNTPSLMENANLAIKQESSWMNQSEHGIKEPQLDTSDIPPFKDEGAESTHYADLWLLNDLKTNDPYRSLSNSSTATGTTVIECIKSPESSESQTSQSESRATTPSLPSVDNEFKLASPEKLAGLASPSSGYSSQSETPTSSFPTAFFSGPLSPGGSKRKPKVPERKSSLQQPSLKDGALSLSKDLELPIIPPTHLDLSALHSVLNKPFHHRHPLHVFTHSKQNPVGETLRSNPPPSLAITPTVLKSVNLRSISKSEEVKPKEGNNTDLPYLEDNTLTMAALSPGKIKPHMAKKSVSRQYSTEDTILSFLDSSAVEMGPDKLQLEKKRTFDVKNHCDPETATTSAGSNLLDSSVTKDQIQMESEPIPENTPSKNCDFPTEGFQMVSVAHPNDLDGKVLQYGGGPDGAVAQLQKPSSADWEGAAHPESVDVLTAQSNSPTRPTDISSQLKHQLGMSRHHDKVPGNISYEAELSSANSCPEKCSEQENIASGISAQSASDNSGAEETQGSVDEVSLKESSPSDDSMISPLSEDSQAEAEGVFVSPNKPRTTEDLFAVIHRSKRKVLGRKDSGDMSARSKSRASLGSSSSSNAGSVTLPNSSVTSPNSQRSPGLIYRNAKKSNTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPILPKPPGELTAESPQSTQEAHQGAPGAEALSPLSPCSPRVNAEGFSSKNFATSAAARVGRSRAPPAASSSRYSVRCRLYNAPMQAISEGETENSDGSPHDDRSSQSST

Target ORF information:

RefSeq Version	XM_548877.4
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Nance-Horan syndrome (congenital cataracts and dental anomalies) (NHS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_548877.4

ORF Insert Sequence:

ATGCCTTTCG CCAAGAGGAT CGTGGAGCCG CAGTGGCTGT GCCGGCAGCG GCGCCCGGCG 
CCCGGCCCGG CGGAGGACGC GAACGGAGGC AGCGCTGACC CGCCGCCGCC CCTGCAGCCG 
CCCGGCCGAC GGGACGAGGC CGTGGCGCCG GGGCCGGAGG ATCCTCCCCG CGCGCCCCCC 
GCGCCTCCCG GGCCGCCGCC GCCGCCGCCG CTGCCCGCGC CGACCGACCA GGCGCAGCCG 
CCGCACGGAG AGGCGCCAGC GGCGGGCGAG GAGAGCGCGG CGGGGGTCGC CGAGGCGGCG 
TCCGCGGCCG GCGAGGCGTC CTCGGCGGCG GCGGCGGCCG TGCTGCTCAT GCTGGACCTG 
TGCGCGGTCA GCAACGCCGC GCTGGCCCGC GTCCTCCGGC AGCTCTCGGA CGTGGCCCGG 
CACGCGTGCA GCCTCTTCCA GGAGCTCGAG AGCGACATCC AGCTGACCCA CCGCCGCGTC 
TGGGCGCTGC AGGGCAAGCT CGGCGGCGTG CAGCGCGTCC TCGGCACGCT GGACCCCAAG 
CAGGAGGCAG TGCCTGTATC CAACCTGGAC ATTGAGAGTA AGCTGAGTGT ATACTACCGT 
GCACCATGGC ACCAGCAGAG AAACATCTTC CTCCCAGCCA CAAGGCCACC CTGCGTGGAG 
GAACTGCATC GCCATGCCCG GCAGAGCCTG CAAGCCCTGC GCAGAGAACA CCGGAGCCGA 
AGTGATCGCC GGGAGCAGAG AGCCGCTGCC CCCCTTTCTG TAGCAGCTCC TCCACTGCCA 
GCCTATCCAC CAGCTCACAG CCAGAGGAGG CGTGAAGTTA AGGACCGGCA CTTCTTAACG 
TCCCATCCCC CGGAGGATGA AGATACAGAT GTCATGTTAG GGCAGAGGCC GAAAAACCCA 
GTACACAATA TCCCTTCAAC ACTGGACAAG CAGACCAACT GGAGCAAAGC ACTGCCTCTC 
CCAACACCAG AGGAGAAAAT GAAACAAGAT GCCCAAGTGA TTTCTTCTTG CATTATTCCC 
ATCAATGTCA CTGGAGTTGG TTTTGACAGA GAGGCTAGTA TACGCTGCTC TCTTGTTCAT 
TCACAATCCG TACTACAGCG GAGACGAAAA CTGAGGAGGA GGAAAACCAT CTCGGGTATC 
CCCAGAAGAG TCCAACAAGA AATAGATTCT GATGAATCCC CTGTGGCCAG GGAAAGGAAT 
GTGATTGTGC ACACAAATCC AGACCCTTCC AACACTGTCA ATAGGAGGTC TGGAACCAGG 
GACTCAGAGT GCCAAACCGA AGATATCCTG ATTGCTGCTC CATCCAGAAG GAGAATCAGA 
GCTCAAAGGG GTCAAAGCAT AGCAGCTTCC CTTTCTCATT CTGCTGGCAA CATCTCTGCA 
TTGGCTGACA AAGGTGACAC CATGTTTACA ACTGCAGTGA GCAGCCGTAC AAGATCTCGG 
AGCCTTCCCC GGGAGGGCAA CAGAAGTGGG GATGCTGAGC CCAAAGTTGG TGTTAAACCC 
GCAGCATATG AAGAGGGGGA GCCTTTCATG GGCGACCAAG AAAGAACCCC TAATGATTGC 
AGTGAGGCCC CCAGCAGCCC AAGTGCACAG GAACACCAGC CTGCTTTGGG TCTGGCTTGT 
TCTCAACATC TTCACAGCCC CCAGCACAAG TTAAATGAGA GAGGGAGGTC ACGTCTGTCC 
CGAATGGCTG CTGACTCTGG CAGTTGTGAC ATCTCCTCCA ACTCAGACAC CTTTGGGAGC 
CCCATCCACT GCATCTCCAC GGCAGGTGTC CTCCTCAGCA GCCACATGGA CCAGAAAGAT 
GACCATCAGT CATCCAGTGG CAACTGGAGT GGGAGCAGCT CCACATGCCC CTCACAGACC 
TCAGAGACAA TCCCTCCTGC AGCTTCTCCT CCCCTCACTG GCTCTTCACA CTGTGACTCA 
GAGTTGTCAC TAAACACAGC CCCTCATGCT AATGAGGATG CCAATGTCTT CGTGACAGAG 
CAGTACAATG ACCACTTGGA TAAAGTGAGA GGCCACCGGG CAAACTCTTT TACCTCCACT 
GTGGCGGATC TGCTGGATGA CCCCAACAAT AGCAACACCA GTGACAGTGA GTGGAATTAC 
CTACACCACC ATCACGATGC CTCCTGCCGC CAGGATTTTA GTCCTGAGCG CCCTAAGGCA 
GACAGCCTGG GCTGCCCGAG CTTCACAAGC ATGGCCACTT ACGACAGCTT TCTGGAAAAG 
TCTCCATCAG ACAAAGCAGA CACTAGCTCT CACTTTTCAG TGGACACAGA AGGATACTAC 
ACTTCCATGC ACTTTGACTG TGGTCTTAAA GGTAATAAGA GCTATGTGTG TCACTATGCA 
GCCCTGGGCC CAGAGAATAG CCAGGGCATT GGGGTTCCTC CGACTCTTCC GGACTGTACC 
TGGCAGGACT ACTTAGACCA CAGGAGGCAG GGGAGACCAA GCATCTCTTT CAGGAAACCA 
AAGGCAAAGC CGACCCCACC TAAACGTAGC TCATCTTTGA GGAAGTCCGA TGGAAATGCA 
GACATTTCTG AGAAGAAAGA ACCAAAGATA AGCAGTGGCC AGCTCCTGCC TCACAGTTCC 
AGGGAAATGA AGCTGCCTCT TGATTTCTCC AACACGCCTT CTCTAATGGA AAATGCCAAC 
CTTGCCATCA AGCAAGAGTC TTCTTGGATG AACCAGAGTG AACATGGTAT CAAGGAACCT 
CAGTTAGACA CTTCAGATAT TCCGCCATTC AAAGATGAAG GTGCTGAATC GACTCACTAT 
GCAGATCTCT GGCTTCTAAA TGACTTGAAA ACAAATGATC CTTACAGATC TTTATCCAAT 
TCAAGCACTG CTACGGGTAC CACAGTTATT GAATGCATCA AGTCTCCAGA GAGCTCTGAA 
TCCCAAACAT CCCAGTCAGA ATCAAGAGCC ACCACCCCAT CCCTTCCTTC TGTTGACAAT 
GAGTTTAAAC TGGCTTCACC AGAAAAGCTG GCAGGCTTGG CATCTCCATC AAGTGGCTAC 
TCGAGCCAGT CTGAAACACC GACCTCCTCT TTCCCTACAG CTTTCTTTTC TGGCCCACTG 
TCTCCTGGAG GTAGCAAAAG AAAACCGAAA GTTCCAGAAA GAAAATCCTC ACTACAGCAG 
CCCTCTTTAA AAGATGGAGC TCTATCACTG AGTAAAGACC TTGAACTTCC AATTATACCT 
CCTACCCATC TTGACCTAAG TGCTCTTCAT AGTGTCTTGA ATAAACCATT CCATCACCGT 
CACCCATTGC ATGTTTTTAC TCATAGTAAA CAGAACCCAG TAGGAGAAAC ACTGCGGTCA 
AATCCTCCAC CATCCCTTGC AATCACACCA ACGGTCCTGA AATCTGTTAA CCTGAGGTCC 
ATCAGTAAAT CTGAAGAAGT TAAGCCAAAA GAGGGCAACA ATACAGATCT CCCCTACTTG 
GAGGACAACA CTCTCACAAT GGCTGCCCTG TCTCCAGGTA AGATTAAGCC ACACATGGCT 
AAGAAATCAG TATCACGTCA GTATTCCACT GAAGACACCA TACTGTCCTT TTTAGACTCC 
TCTGCAGTTG AGATGGGACC AGATAAACTA CAGTTAGAGA AAAAACGTAC TTTTGATGTA 
AAGAATCATT GTGACCCAGA AACAGCAACA ACCTCAGCTG GTAGCAATCT TCTAGATTCA 
AGTGTGACAA AAGACCAAAT ACAAATGGAA AGTGAGCCTA TTCCAGAAAA CACACCAAGT 
AAAAATTGTG ACTTTCCCAC AGAAGGATTT CAGATGGTCT CTGTTGCCCA CCCAAATGAT 
TTGGATGGTA AAGTATTACA ATATGGAGGT GGTCCAGATG GAGCCGTTGC ACAACTGCAG 
AAGCCATCCT CTGCAGATTG GGAGGGAGCC GCACACCCTG AGTCTGTGGA TGTGCTCACA 
GCTCAGTCAA ACTCGCCAAC CAGACCCACA GACATAAGCA GTCAACTTAA GCATCAACTT 
GGGATGAGCC GCCACCATGA CAAAGTGCCT GGGAATATCA GCTATGAAGC AGAGCTATCA 
AGTGCAAATT CATGCCCTGA AAAATGTTCC GAGCAAGAAA ATATCGCTTC AGGTATTTCA 
GCCCAAAGTG CCTCTGATAA CAGCGGAGCA GAGGAGACCC AAGGAAGTGT GGACGAGGTT 
TCACTGAAAG AATCGTCACC GAGTGATGAC TCTATGATTT CGCCACTTAG TGAAGACTCT 
CAGGCTGAAG CAGAAGGTGT GTTTGTGTCT CCAAACAAAC CTCGTACAAC TGAAGATTTG 
TTTGCAGTCA TTCACAGGTC GAAGAGGAAA GTACTTGGAA GAAAAGATTC TGGGGATATG 
TCTGCTCGAA GCAAATCAAG AGCTTCTCTT GGCAGCAGTA GCAGCAGCAA CGCTGGCTCT 
GTCACTTTGC CCAACAGCAG CGTGACCAGC CCAAACAGCC AGAGGTCTCC TGGCCTCATC 
TACCGAAATG CCAAAAAGTC CAACACATCG AATGAAGAGT TTAAGCTGTT ACTCCTCAAG 
AAAGGAAGTC GCTCAGATTC TAGTTACCGC ATGTCTGCTA CTGAGATCCT GAAAAGTCCC 
ATCCTGCCCA AACCTCCTGG AGAGCTCACA GCTGAATCCC CACAAAGCAC CCAGGAGGCC 
CATCAGGGGG CACCTGGGGC CGAGGCTTTG TCCCCACTCT CTCCATGCTC CCCAAGAGTT 
AATGCAGAAG GGTTTTCCTC AAAGAACTTT GCCACCTCAG CAGCAGCAAG GGTGGGACGC 
TCCCGAGCTC CCCCTGCGGC CAGCAGCAGC CGCTACAGCG TCCGCTGCAG GCTGTACAAT 
GCACCCATGC AGGCCATCTC TGAGGGTGAG ACAGAAAATT CTGATGGAAG CCCACATGAT 
GACCGATCCT CCCAGAGCTC AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHS ( XM_548877.5 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_548877.2 Canis lupus familiaris NHS actin remodeling regulator (NHS), transcript variant X2, mRNA.

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CloneID	OCe32122
Clone ID Related Accession (Same CDS sequence)	XM_548877.4 , XM_548877.5
Accession Version	XM_548877.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Nance-Horan syndrome protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_548877.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTCG CCAAGAGGAT CGTGGAGCCG CAGTGGCTGT GCCGGCAGCG GCGCCCGGCG 
CCCGGCCCGG CGGAGGACGC GAACGGAGGC AGCGCTGACC CGCCGCCGCC CCTGCAGCCG 
CCCGGCCGAC GGGACGAGGC CGTGGCGCCG GGGCCGGAGG ATCCTCCCCG CGCGCCCCCC 
GCGCCTCCCG GGCCGCCGCC GCCGCCGCCG CTGCCCGCGC CGACCGACCA GGCGCAGCCG 
CCGCACGGAG AGGCGCCAGC GGCGGGCGAG GAGAGCGCGG CGGGGGTCGC CGAGGCGGCG 
TCCGCGGCCG GCGAGGCGTC CTCGGCGGCG GCGGCGGCCG TGCTGCTCAT GCTGGACCTG 
TGCGCGGTCA GCAACGCCGC GCTGGCCCGC GTCCTCCGGC AGCTCTCGGA CGTGGCCCGG 
CACGCGTGCA GCCTCTTCCA GGAGCTCGAG AGCGACATCC AGCTGACCCA CCGCCGCGTC 
TGGGCGCTGC AGGGCAAGCT CGGCGGCGTG CAGCGCGTCC TCGGCACGCT GGACCCCAAG 
CAGGAGGCAG TGCCTGTATC CAACCTGGAC ATTGAGAGTA AGCTGAGTGT ATACTACCGT 
GCACCATGGC ACCAGCAGAG AAACATCTTC CTCCCAGCCA CAAGGCCACC CTGCGTGGAG 
GAACTGCATC GCCATGCCCG GCAGAGCCTG CAAGCCCTGC GCAGAGAACA CCGGAGCCGA 
AGTGATCGCC GGGAGCAGAG AGCCGCTGCC CCCCTTTCTG TAGCAGCTCC TCCACTGCCA 
GCCTATCCAC CAGCTCACAG CCAGAGGAGG CGTGAAGTTA AGGACCGGCA CTTCTTAACG 
TCCCATCCCC CGGAGGATGA AGATACAGAT GTCATGTTAG GGCAGAGGCC GAAAAACCCA 
GTACACAATA TCCCTTCAAC ACTGGACAAG CAGACCAACT GGAGCAAAGC ACTGCCTCTC 
CCAACACCAG AGGAGAAAAT GAAACAAGAT GCCCAAGTGA TTTCTTCTTG CATTATTCCC 
ATCAATGTCA CTGGAGTTGG TTTTGACAGA GAGGCTAGTA TACGCTGCTC TCTTGTTCAT 
TCACAATCCG TACTACAGCG GAGACGAAAA CTGAGGAGGA GGAAAACCAT CTCGGGTATC 
CCCAGAAGAG TCCAACAAGA AATAGATTCT GATGAATCCC CTGTGGCCAG GGAAAGGAAT 
GTGATTGTGC ACACAAATCC AGACCCTTCC AACACTGTCA ATAGGAGGTC TGGAACCAGG 
GACTCAGAGT GCCAAACCGA AGATATCCTG ATTGCTGCTC CATCCAGAAG GAGAATCAGA 
GCTCAAAGGG GTCAAAGCAT AGCAGCTTCC CTTTCTCATT CTGCTGGCAA CATCTCTGCA 
TTGGCTGACA AAGGTGACAC CATGTTTACA ACTGCAGTGA GCAGCCGTAC AAGATCTCGG 
AGCCTTCCCC GGGAGGGCAA CAGAAGTGGG GATGCTGAGC CCAAAGTTGG TGTTAAACCC 
GCAGCATATG AAGAGGGGGA GCCTTTCATG GGCGACCAAG AAAGAACCCC TAATGATTGC 
AGTGAGGCCC CCAGCAGCCC AAGTGCACAG GAACACCAGC CTGCTTTGGG TCTGGCTTGT 
TCTCAACATC TTCACAGCCC CCAGCACAAG TTAAATGAGA GAGGGAGGTC ACGTCTGTCC 
CGAATGGCTG CTGACTCTGG CAGTTGTGAC ATCTCCTCCA ACTCAGACAC CTTTGGGAGC 
CCCATCCACT GCATCTCCAC GGCAGGTGTC CTCCTCAGCA GCCACATGGA CCAGAAAGAT 
GACCATCAGT CATCCAGTGG CAACTGGAGT GGGAGCAGCT CCACATGCCC CTCACAGACC 
TCAGAGACAA TCCCTCCTGC AGCTTCTCCT CCCCTCACTG GCTCTTCACA CTGTGACTCA 
GAGTTGTCAC TAAACACAGC CCCTCATGCT AATGAGGATG CCAATGTCTT CGTGACAGAG 
CAGTACAATG ACCACTTGGA TAAAGTGAGA GGCCACCGGG CAAACTCTTT TACCTCCACT 
GTGGCGGATC TGCTGGATGA CCCCAACAAT AGCAACACCA GTGACAGTGA GTGGAATTAC 
CTACACCACC ATCACGATGC CTCCTGCCGC CAGGATTTTA GTCCTGAGCG CCCTAAGGCA 
GACAGCCTGG GCTGCCCGAG CTTCACAAGC ATGGCCACTT ACGACAGCTT TCTGGAAAAG 
TCTCCATCAG ACAAAGCAGA CACTAGCTCT CACTTTTCAG TGGACACAGA AGGATACTAC 
ACTTCCATGC ACTTTGACTG TGGTCTTAAA GGTAATAAGA GCTATGTGTG TCACTATGCA 
GCCCTGGGCC CAGAGAATAG CCAGGGCATT GGGGTTCCTC CGACTCTTCC GGACTGTACC 
TGGCAGGACT ACTTAGACCA CAGGAGGCAG GGGAGACCAA GCATCTCTTT CAGGAAACCA 
AAGGCAAAGC CGACCCCACC TAAACGTAGC TCATCTTTGA GGAAGTCCGA TGGAAATGCA 
GACATTTCTG AGAAGAAAGA ACCAAAGATA AGCAGTGGCC AGCTCCTGCC TCACAGTTCC 
AGGGAAATGA AGCTGCCTCT TGATTTCTCC AACACGCCTT CTCTAATGGA AAATGCCAAC 
CTTGCCATCA AGCAAGAGTC TTCTTGGATG AACCAGAGTG AACATGGTAT CAAGGAACCT 
CAGTTAGACA CTTCAGATAT TCCGCCATTC AAAGATGAAG GTGCTGAATC GACTCACTAT 
GCAGATCTCT GGCTTCTAAA TGACTTGAAA ACAAATGATC CTTACAGATC TTTATCCAAT 
TCAAGCACTG CTACGGGTAC CACAGTTATT GAATGCATCA AGTCTCCAGA GAGCTCTGAA 
TCCCAAACAT CCCAGTCAGA ATCAAGAGCC ACCACCCCAT CCCTTCCTTC TGTTGACAAT 
GAGTTTAAAC TGGCTTCACC AGAAAAGCTG GCAGGCTTGG CATCTCCATC AAGTGGCTAC 
TCGAGCCAGT CTGAAACACC GACCTCCTCT TTCCCTACAG CTTTCTTTTC TGGCCCACTG 
TCTCCTGGAG GTAGCAAAAG AAAACCGAAA GTTCCAGAAA GAAAATCCTC ACTACAGCAG 
CCCTCTTTAA AAGATGGAGC TCTATCACTG AGTAAAGACC TTGAACTTCC AATTATACCT 
CCTACCCATC TTGACCTAAG TGCTCTTCAT AGTGTCTTGA ATAAACCATT CCATCACCGT 
CACCCATTGC ATGTTTTTAC TCATAGTAAA CAGAACCCAG TAGGAGAAAC ACTGCGGTCA 
AATCCTCCAC CATCCCTTGC AATCACACCA ACGGTCCTGA AATCTGTTAA CCTGAGGTCC 
ATCAGTAAAT CTGAAGAAGT TAAGCCAAAA GAGGGCAACA ATACAGATCT CCCCTACTTG 
GAGGACAACA CTCTCACAAT GGCTGCCCTG TCTCCAGGTA AGATTAAGCC ACACATGGCT 
AAGAAATCAG TATCACGTCA GTATTCCACT GAAGACACCA TACTGTCCTT TTTAGACTCC 
TCTGCAGTTG AGATGGGACC AGATAAACTA CAGTTAGAGA AAAAACGTAC TTTTGATGTA 
AAGAATCATT GTGACCCAGA AACAGCAACA ACCTCAGCTG GTAGCAATCT TCTAGATTCA 
AGTGTGACAA AAGACCAAAT ACAAATGGAA AGTGAGCCTA TTCCAGAAAA CACACCAAGT 
AAAAATTGTG ACTTTCCCAC AGAAGGATTT CAGATGGTCT CTGTTGCCCA CCCAAATGAT 
TTGGATGGTA AAGTATTACA ATATGGAGGT GGTCCAGATG GAGCCGTTGC ACAACTGCAG 
AAGCCATCCT CTGCAGATTG GGAGGGAGCC GCACACCCTG AGTCTGTGGA TGTGCTCACA 
GCTCAGTCAA ACTCGCCAAC CAGACCCACA GACATAAGCA GTCAACTTAA GCATCAACTT 
GGGATGAGCC GCCACCATGA CAAAGTGCCT GGGAATATCA GCTATGAAGC AGAGCTATCA 
AGTGCAAATT CATGCCCTGA AAAATGTTCC GAGCAAGAAA ATATCGCTTC AGGTATTTCA 
GCCCAAAGTG CCTCTGATAA CAGCGGAGCA GAGGAGACCC AAGGAAGTGT GGACGAGGTT 
TCACTGAAAG AATCGTCACC GAGTGATGAC TCTATGATTT CGCCACTTAG TGAAGACTCT 
CAGGCTGAAG CAGAAGGTGT GTTTGTGTCT CCAAACAAAC CTCGTACAAC TGAAGATTTG 
TTTGCAGTCA TTCACAGGTC GAAGAGGAAA GTACTTGGAA GAAAAGATTC TGGGGATATG 
TCTGCTCGAA GCAAATCAAG AGCTTCTCTT GGCAGCAGTA GCAGCAGCAA CGCTGGCTCT 
GTCACTTTGC CCAACAGCAG CGTGACCAGC CCAAACAGCC AGAGGTCTCC TGGCCTCATC 
TACCGAAATG CCAAAAAGTC CAACACATCG AATGAAGAGT TTAAGCTGTT ACTCCTCAAG 
AAAGGAAGTC GCTCAGATTC TAGTTACCGC ATGTCTGCTA CTGAGATCCT GAAAAGTCCC 
ATCCTGCCCA AACCTCCTGG AGAGCTCACA GCTGAATCCC CACAAAGCAC CCAGGAGGCC 
CATCAGGGGG CACCTGGGGC CGAGGCTTTG TCCCCACTCT CTCCATGCTC CCCAAGAGTT 
AATGCAGAAG GGTTTTCCTC AAAGAACTTT GCCACCTCAG CAGCAGCAAG GGTGGGACGC 
TCCCGAGCTC CCCCTGCGGC CAGCAGCAGC CGCTACAGCG TCCGCTGCAG GCTGTACAAT 
GCACCCATGC AGGCCATCTC TGAGGGTGAG ACAGAAAATT CTGATGGAAG CCCACATGAT 
GACCGATCCT CCCAGAGCTC AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_548877.2
CDS	597..5483
Translation	MPFAKRIVEPQWLCRQRRPAPGPAEDANGGSADPPPPLQPPGRRDEAVAPGPEDPPRAPPAPPGPPPPPPLPAPTDQAQPPHGEAPAAGEESAAGVAEAASAAGEASSAAAAAVLLMLDLCAVSNAALARVLRQLSDVARHACSLFQELESDIQLTHRRVWALQGKLGGVQRVLGTLDPKQEAVPVSNLDIESKLSVYYRAPWHQQRNIFLPATRPPCVEELHRHARQSLQALRREHRSRSDRREQRAAAPLSVAAPPLPAYPPAHSQRRREVKDRHFLTSHPPEDEDTDVMLGQRPKNPVHNIPSTLDKQTNWSKALPLPTPEEKMKQDAQVISSCIIPINVTGVGFDREASIRCSLVHSQSVLQRRRKLRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPDPSNTVNRRSGTRDSECQTEDILIAAPSRRRIRAQRGQSIAASLSHSAGNISALADKGDTMFTTAVSSRTRSRSLPREGNRSGDAEPKVGVKPAAYEEGEPFMGDQERTPNDCSEAPSSPSAQEHQPALGLACSQHLHSPQHKLNERGRSRLSRMAADSGSCDISSNSDTFGSPIHCISTAGVLLSSHMDQKDDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNTAPHANEDANVFVTEQYNDHLDKVRGHRANSFTSTVADLLDDPNNSNTSDSEWNYLHHHHDASCRQDFSPERPKADSLGCPSFTSMATYDSFLEKSPSDKADTSSHFSVDTEGYYTSMHFDCGLKGNKSYVCHYAALGPENSQGIGVPPTLPDCTWQDYLDHRRQGRPSISFRKPKAKPTPPKRSSSLRKSDGNADISEKKEPKISSGQLLPHSSREMKLPLDFSNTPSLMENANLAIKQESSWMNQSEHGIKEPQLDTSDIPPFKDEGAESTHYADLWLLNDLKTNDPYRSLSNSSTATGTTVIECIKSPESSESQTSQSESRATTPSLPSVDNEFKLASPEKLAGLASPSSGYSSQSETPTSSFPTAFFSGPLSPGGSKRKPKVPERKSSLQQPSLKDGALSLSKDLELPIIPPTHLDLSALHSVLNKPFHHRHPLHVFTHSKQNPVGETLRSNPPPSLAITPTVLKSVNLRSISKSEEVKPKEGNNTDLPYLEDNTLTMAALSPGKIKPHMAKKSVSRQYSTEDTILSFLDSSAVEMGPDKLQLEKKRTFDVKNHCDPETATTSAGSNLLDSSVTKDQIQMESEPIPENTPSKNCDFPTEGFQMVSVAHPNDLDGKVLQYGGGPDGAVAQLQKPSSADWEGAAHPESVDVLTAQSNSPTRPTDISSQLKHQLGMSRHHDKVPGNISYEAELSSANSCPEKCSEQENIASGISAQSASDNSGAEETQGSVDEVSLKESSPSDDSMISPLSEDSQAEAEGVFVSPNKPRTTEDLFAVIHRSKRKVLGRKDSGDMSARSKSRASLGSSSSSNAGSVTLPNSSVTSPNSQRSPGLIYRNAKKSNTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPILPKPPGELTAESPQSTQEAHQGAPGAEALSPLSPCSPRVNAEGFSSKNFATSAAARVGRSRAPPAASSSRYSVRCRLYNAPMQAISEGETENSDGSPHDDRSSQSST

Target ORF information:

RefSeq Version	XM_548877.5
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NHS actin remodeling regulator (NHS), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_548877.5

ORF Insert Sequence:

ATGCCTTTCG CCAAGAGGAT CGTGGAGCCG CAGTGGCTGT GCCGGCAGCG GCGCCCGGCG 
CCCGGCCCGG CGGAGGACGC GAACGGAGGC AGCGCTGACC CGCCGCCGCC CCTGCAGCCG 
CCCGGCCGAC GGGACGAGGC CGTGGCGCCG GGGCCGGAGG ATCCTCCCCG CGCGCCCCCC 
GCGCCTCCCG GGCCGCCGCC GCCGCCGCCG CTGCCCGCGC CGACCGACCA GGCGCAGCCG 
CCGCACGGAG AGGCGCCAGC GGCGGGCGAG GAGAGCGCGG CGGGGGTCGC CGAGGCGGCG 
TCCGCGGCCG GCGAGGCGTC CTCGGCGGCG GCGGCGGCCG TGCTGCTCAT GCTGGACCTG 
TGCGCGGTCA GCAACGCCGC GCTGGCCCGC GTCCTCCGGC AGCTCTCGGA CGTGGCCCGG 
CACGCGTGCA GCCTCTTCCA GGAGCTCGAG AGCGACATCC AGCTGACCCA CCGCCGCGTC 
TGGGCGCTGC AGGGCAAGCT CGGCGGCGTG CAGCGCGTCC TCGGCACGCT GGACCCCAAG 
CAGGAGGCAG TGCCTGTATC CAACCTGGAC ATTGAGAGTA AGCTGAGTGT ATACTACCGT 
GCACCATGGC ACCAGCAGAG AAACATCTTC CTCCCAGCCA CAAGGCCACC CTGCGTGGAG 
GAACTGCATC GCCATGCCCG GCAGAGCCTG CAAGCCCTGC GCAGAGAACA CCGGAGCCGA 
AGTGATCGCC GGGAGCAGAG AGCCGCTGCC CCCCTTTCTG TAGCAGCTCC TCCACTGCCA 
GCCTATCCAC CAGCTCACAG CCAGAGGAGG CGTGAAGTTA AGGACCGGCA CTTCTTAACG 
TCCCATCCCC CGGAGGATGA AGATACAGAT GTCATGTTAG GGCAGAGGCC GAAAAACCCA 
GTACACAATA TCCCTTCAAC ACTGGACAAG CAGACCAACT GGAGCAAAGC ACTGCCTCTC 
CCAACACCAG AGGAGAAAAT GAAACAAGAT GCCCAAGTGA TTTCTTCTTG CATTATTCCC 
ATCAATGTCA CTGGAGTTGG TTTTGACAGA GAGGCTAGTA TACGCTGCTC TCTTGTTCAT 
TCACAATCCG TACTACAGCG GAGACGAAAA CTGAGGAGGA GGAAAACCAT CTCGGGTATC 
CCCAGAAGAG TCCAACAAGA AATAGATTCT GATGAATCCC CTGTGGCCAG GGAAAGGAAT 
GTGATTGTGC ACACAAATCC AGACCCTTCC AACACTGTCA ATAGGAGGTC TGGAACCAGG 
GACTCAGAGT GCCAAACCGA AGATATCCTG ATTGCTGCTC CATCCAGAAG GAGAATCAGA 
GCTCAAAGGG GTCAAAGCAT AGCAGCTTCC CTTTCTCATT CTGCTGGCAA CATCTCTGCA 
TTGGCTGACA AAGGTGACAC CATGTTTACA ACTGCAGTGA GCAGCCGTAC AAGATCTCGG 
AGCCTTCCCC GGGAGGGCAA CAGAAGTGGG GATGCTGAGC CCAAAGTTGG TGTTAAACCC 
GCAGCATATG AAGAGGGGGA GCCTTTCATG GGCGACCAAG AAAGAACCCC TAATGATTGC 
AGTGAGGCCC CCAGCAGCCC AAGTGCACAG GAACACCAGC CTGCTTTGGG TCTGGCTTGT 
TCTCAACATC TTCACAGCCC CCAGCACAAG TTAAATGAGA GAGGGAGGTC ACGTCTGTCC 
CGAATGGCTG CTGACTCTGG CAGTTGTGAC ATCTCCTCCA ACTCAGACAC CTTTGGGAGC 
CCCATCCACT GCATCTCCAC GGCAGGTGTC CTCCTCAGCA GCCACATGGA CCAGAAAGAT 
GACCATCAGT CATCCAGTGG CAACTGGAGT GGGAGCAGCT CCACATGCCC CTCACAGACC 
TCAGAGACAA TCCCTCCTGC AGCTTCTCCT CCCCTCACTG GCTCTTCACA CTGTGACTCA 
GAGTTGTCAC TAAACACAGC CCCTCATGCT AATGAGGATG CCAATGTCTT CGTGACAGAG 
CAGTACAATG ACCACTTGGA TAAAGTGAGA GGCCACCGGG CAAACTCTTT TACCTCCACT 
GTGGCGGATC TGCTGGATGA CCCCAACAAT AGCAACACCA GTGACAGTGA GTGGAATTAC 
CTACACCACC ATCACGATGC CTCCTGCCGC CAGGATTTTA GTCCTGAGCG CCCTAAGGCA 
GACAGCCTGG GCTGCCCGAG CTTCACAAGC ATGGCCACTT ACGACAGCTT TCTGGAAAAG 
TCTCCATCAG ACAAAGCAGA CACTAGCTCT CACTTTTCAG TGGACACAGA AGGATACTAC 
ACTTCCATGC ACTTTGACTG TGGTCTTAAA GGTAATAAGA GCTATGTGTG TCACTATGCA 
GCCCTGGGCC CAGAGAATAG CCAGGGCATT GGGGTTCCTC CGACTCTTCC GGACTGTACC 
TGGCAGGACT ACTTAGACCA CAGGAGGCAG GGGAGACCAA GCATCTCTTT CAGGAAACCA 
AAGGCAAAGC CGACCCCACC TAAACGTAGC TCATCTTTGA GGAAGTCCGA TGGAAATGCA 
GACATTTCTG AGAAGAAAGA ACCAAAGATA AGCAGTGGCC AGCTCCTGCC TCACAGTTCC 
AGGGAAATGA AGCTGCCTCT TGATTTCTCC AACACGCCTT CTCTAATGGA AAATGCCAAC 
CTTGCCATCA AGCAAGAGTC TTCTTGGATG AACCAGAGTG AACATGGTAT CAAGGAACCT 
CAGTTAGACA CTTCAGATAT TCCGCCATTC AAAGATGAAG GTGCTGAATC GACTCACTAT 
GCAGATCTCT GGCTTCTAAA TGACTTGAAA ACAAATGATC CTTACAGATC TTTATCCAAT 
TCAAGCACTG CTACGGGTAC CACAGTTATT GAATGCATCA AGTCTCCAGA GAGCTCTGAA 
TCCCAAACAT CCCAGTCAGA ATCAAGAGCC ACCACCCCAT CCCTTCCTTC TGTTGACAAT 
GAGTTTAAAC TGGCTTCACC AGAAAAGCTG GCAGGCTTGG CATCTCCATC AAGTGGCTAC 
TCGAGCCAGT CTGAAACACC GACCTCCTCT TTCCCTACAG CTTTCTTTTC TGGCCCACTG 
TCTCCTGGAG GTAGCAAAAG AAAACCGAAA GTTCCAGAAA GAAAATCCTC ACTACAGCAG 
CCCTCTTTAA AAGATGGAGC TCTATCACTG AGTAAAGACC TTGAACTTCC AATTATACCT 
CCTACCCATC TTGACCTAAG TGCTCTTCAT AGTGTCTTGA ATAAACCATT CCATCACCGT 
CACCCATTGC ATGTTTTTAC TCATAGTAAA CAGAACCCAG TAGGAGAAAC ACTGCGGTCA 
AATCCTCCAC CATCCCTTGC AATCACACCA ACGGTCCTGA AATCTGTTAA CCTGAGGTCC 
ATCAGTAAAT CTGAAGAAGT TAAGCCAAAA GAGGGCAACA ATACAGATCT CCCCTACTTG 
GAGGACAACA CTCTCACAAT GGCTGCCCTG TCTCCAGGTA AGATTAAGCC ACACATGGCT 
AAGAAATCAG TATCACGTCA GTATTCCACT GAAGACACCA TACTGTCCTT TTTAGACTCC 
TCTGCAGTTG AGATGGGACC AGATAAACTA CAGTTAGAGA AAAAACGTAC TTTTGATGTA 
AAGAATCATT GTGACCCAGA AACAGCAACA ACCTCAGCTG GTAGCAATCT TCTAGATTCA 
AGTGTGACAA AAGACCAAAT ACAAATGGAA AGTGAGCCTA TTCCAGAAAA CACACCAAGT 
AAAAATTGTG ACTTTCCCAC AGAAGGATTT CAGATGGTCT CTGTTGCCCA CCCAAATGAT 
TTGGATGGTA AAGTATTACA ATATGGAGGT GGTCCAGATG GAGCCGTTGC ACAACTGCAG 
AAGCCATCCT CTGCAGATTG GGAGGGAGCC GCACACCCTG AGTCTGTGGA TGTGCTCACA 
GCTCAGTCAA ACTCGCCAAC CAGACCCACA GACATAAGCA GTCAACTTAA GCATCAACTT 
GGGATGAGCC GCCACCATGA CAAAGTGCCT GGGAATATCA GCTATGAAGC AGAGCTATCA 
AGTGCAAATT CATGCCCTGA AAAATGTTCC GAGCAAGAAA ATATCGCTTC AGGTATTTCA 
GCCCAAAGTG CCTCTGATAA CAGCGGAGCA GAGGAGACCC AAGGAAGTGT GGACGAGGTT 
TCACTGAAAG AATCGTCACC GAGTGATGAC TCTATGATTT CGCCACTTAG TGAAGACTCT 
CAGGCTGAAG CAGAAGGTGT GTTTGTGTCT CCAAACAAAC CTCGTACAAC TGAAGATTTG 
TTTGCAGTCA TTCACAGGTC GAAGAGGAAA GTACTTGGAA GAAAAGATTC TGGGGATATG 
TCTGCTCGAA GCAAATCAAG AGCTTCTCTT GGCAGCAGTA GCAGCAGCAA CGCTGGCTCT 
GTCACTTTGC CCAACAGCAG CGTGACCAGC CCAAACAGCC AGAGGTCTCC TGGCCTCATC 
TACCGAAATG CCAAAAAGTC CAACACATCG AATGAAGAGT TTAAGCTGTT ACTCCTCAAG 
AAAGGAAGTC GCTCAGATTC TAGTTACCGC ATGTCTGCTA CTGAGATCCT GAAAAGTCCC 
ATCCTGCCCA AACCTCCTGG AGAGCTCACA GCTGAATCCC CACAAAGCAC CCAGGAGGCC 
CATCAGGGGG CACCTGGGGC CGAGGCTTTG TCCCCACTCT CTCCATGCTC CCCAAGAGTT 
AATGCAGAAG GGTTTTCCTC AAAGAACTTT GCCACCTCAG CAGCAGCAAG GGTGGGACGC 
TCCCGAGCTC CCCCTGCGGC CAGCAGCAGC CGCTACAGCG TCCGCTGCAG GCTGTACAAT 
GCACCCATGC AGGCCATCTC TGAGGGTGAG ACAGAAAATT CTGATGGAAG CCCACATGAT 
GACCGATCCT CCCAGAGCTC AACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHS ( XM_014111688.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967163.1 Canis lupus familiaris Nance-Horan syndrome (congenital cataracts and dental anomalies) (NHS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe45625
Clone ID Related Accession (Same CDS sequence)	XM_014111688.1 , XM_014111688.2
Accession Version	XM_014111688.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	Nance-Horan syndrome protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTCG CCAAGAGGAT CGTGGAGCCG CAGTGGCTGT GCCGGCAGCG GCGCCCGGCG 
CCCGGCCCGG CGGAGGACGC GAACGGAGGC AGCGCTGACC CGCCGCCGCC CCTGCAGCCG 
CCCGGCCGAC GGGACGAGGC CGTGGCGCCG GGGCCGGAGG ATCCTCCCCG CGCGCCCCCC 
GCGCCTCCCG GGCCGCCGCC GCCGCCGCCG CTGCCCGCGC CGACCGACCA GGCGCAGCCG 
CCGCACGGAG AGGCGCCAGC GGCGGGCGAG GAGAGCGCGG CGGGGGTCGC CGAGGCGGCG 
TCCGCGGCCG GCGAGGCGTC CTCGGCGGCG GCGGCGGCCG TGCTGCTCAT GCTGGACCTG 
TGCGCGGTCA GCAACGCCGC GCTGGCCCGC GTCCTCCGGC AGCTCTCGGA CGTGGCCCGG 
CACGCGTGCA GCCTCTTCCA GGAGCTCGAG AGCGACATCC AGCTGACCCA CCGCCGCGTC 
TGGGCGCTGC AGGGCAAGCT CGGCGGCGTG CAGCGCGTCC TCGGCACGCT GGACCCCAAG 
CAGGAGGCAG TGCCTGTATC CAACCTGGAC ATTGAGAGTA AGCTGAGTGT ATACTACCGT 
GCACCATGGC ACCAGCAGAG AAACATCTTC CTCCCAGCCA CAAGGCCACC CTGCGTGGAG 
GAACTGCATC GCCATGCCCG GCAGAGCCTG CAAGCCCTGC GCAGAGAACA CCGGAGCCGA 
AGTGATCGCC GGGAGCAGAG AGCCGCTGCC CCCCTTTCTG TAGCAGCTCC TCCACTGCCA 
GCCTATCCAC CAGCTCACAG CCAGAGGAGG CGTGAAGTTA AGGACCGGCA CTTCTTAACG 
TTTAACAGCA CCCGTTCGCC CTCCCCCACT GAGTGTTGCC ACATGACCCC ATGGAGTAGA 
AAGTCCCATC CCCCGGAGGA TGAAGATACA GATGTCATGT TAGGGCAGAG GCCGAAAAAC 
CCAGTACACA ATATCCCTTC AACACTGGAC AAGCAGACCA ACTGGAGCAA AGCACTGCCT 
CTCCCAACAC CAGAGGAGAA AATGAAACAA GATGCCCAAG TGATTTCTTC TTGCATTATT 
CCCATCAATG TCACTGGAGT TGGTTTTGAC AGAGAGGCTA GTATACGCTG CTCTCTTGTT 
CATTCACAAT CCGTACTACA GCGGAGACGA AAACTGAGGA GGAGGAAAAC CATCTCGGGT 
ATCCCCAGAA GAGTCCAACA AGAAATAGAT TCTGATGAAT CCCCTGTGGC CAGGGAAAGG 
AATGTGATTG TGCACACAAA TCCAGACCCT TCCAACACTG TCAATAGGAG GTCTGGAACC 
AGGGACTCAG AGTGCCAAAC CGAAGATATC CTGATTGCTG CTCCATCCAG AAGGAGAATC 
AGAGCTCAAA GGGGTCAAAG CATAGCAGCT TCCCTTTCTC ATTCTGCTGG CAACATCTCT 
GCATTGGCTG ACAAAGGTGA CACCATGTTT ACAACTGCAG TGAGCAGCCG TACAAGATCT 
CGGAGCCTTC CCCGGGAGGG CAACAGAAGT GGGGATGCTG AGCCCAAAGT TGGTGTTAAA 
CCCGCAGCAT ATGAAGAGGG GGAGCCTTTC ATGGGCGACC AAGAAAGAAC CCCTAATGAT 
TGCAGTGAGG CCCCCAGCAG CCCAAGTGCA CAGGAACACC AGCCTGCTTT GGGTCTGGCT 
TGTTCTCAAC ATCTTCACAG CCCCCAGCAC AAGTTAAATG AGAGAGGGAG GTCACGTCTG 
TCCCGAATGG CTGCTGACTC TGGCAGTTGT GACATCTCCT CCAACTCAGA CACCTTTGGG 
AGCCCCATCC ACTGCATCTC CACGGCAGGT GTCCTCCTCA GCAGCCACAT GGACCAGAAA 
GATGACCATC AGTCATCCAG TGGCAACTGG AGTGGGAGCA GCTCCACATG CCCCTCACAG 
ACCTCAGAGA CAATCCCTCC TGCAGCTTCT CCTCCCCTCA CTGGCTCTTC ACACTGTGAC 
TCAGAGTTGT CACTAAACAC AGCCCCTCAT GCTAATGAGG ATGCCAATGT CTTCGTGACA 
GAGCAGTACA ATGACCACTT GGATAAAGTG AGAGGCCACC GGGCAAACTC TTTTACCTCC 
ACTGTGGCGG ATCTGCTGGA TGACCCCAAC AATAGCAACA CCAGTGACAG TGAGTGGAAT 
TACCTACACC ACCATCACGA TGCCTCCTGC CGCCAGGATT TTAGTCCTGA GCGCCCTAAG 
GCAGACAGCC TGGGCTGCCC GAGCTTCACA AGCATGGCCA CTTACGACAG CTTTCTGGAA 
AAGTCTCCAT CAGACAAAGC AGACACTAGC TCTCACTTTT CAGTGGACAC AGAAGGATAC 
TACACTTCCA TGCACTTTGA CTGTGGTCTT AAAGGTAATA AGAGCTATGT GTGTCACTAT 
GCAGCCCTGG GCCCAGAGAA TAGCCAGGGC ATTGGGGTTC CTCCGACTCT TCCGGACTGT 
ACCTGGCAGG ACTACTTAGA CCACAGGAGG CAGGGGAGAC CAAGCATCTC TTTCAGGAAA 
CCAAAGGCAA AGCCGACCCC ACCTAAACGT AGCTCATCTT TGAGGAAGTC CGATGGAAAT 
GCAGACATTT CTGAGAAGAA AGAACCAAAG ATAAGCAGTG GCCAGCTCCT GCCTCACAGT 
TCCAGGGAAA TGAAGCTGCC TCTTGATTTC TCCAACACGC CTTCTCTAAT GGAAAATGCC 
AACCTTGCCA TCAAGCAAGA GTCTTCTTGG ATGAACCAGA GTGAACATGG TATCAAGGAA 
CCTCAGTTAG ACACTTCAGA TATTCCGCCA TTCAAAGATG AAGGTGCTGA ATCGACTCAC 
TATGCAGATC TCTGGCTTCT AAATGACTTG AAAACAAATG ATCCTTACAG ATCTTTATCC 
AATTCAAGCA CTGCTACGGG TACCACAGTT ATTGAATGCA TCAAGTCTCC AGAGAGCTCT 
GAATCCCAAA CATCCCAGTC AGAATCAAGA GCCACCACCC CATCCCTTCC TTCTGTTGAC 
AATGAGTTTA AACTGGCTTC ACCAGAAAAG CTGGCAGGCT TGGCATCTCC ATCAAGTGGC 
TACTCGAGCC AGTCTGAAAC ACCGACCTCC TCTTTCCCTA CAGCTTTCTT TTCTGGCCCA 
CTGTCTCCTG GAGGTAGCAA AAGAAAACCG AAAGTTCCAG AAAGAAAATC CTCACTACAG 
CAGCCCTCTT TAAAAGATGG AGCTCTATCA CTGAGTAAAG ACCTTGAACT TCCAATTATA 
CCTCCTACCC ATCTTGACCT AAGTGCTCTT CATAGTGTCT TGAATAAACC ATTCCATCAC 
CGTCACCCAT TGCATGTTTT TACTCATAGT AAACAGAACC CAGTAGGAGA AACACTGCGG 
TCAAATCCTC CACCATCCCT TGCAATCACA CCAACGGTCC TGAAATCTGT TAACCTGAGG 
TCCATCAGTA AATCTGAAGA AGTTAAGCCA AAAGAGGGCA ACAATACAGA TCTCCCCTAC 
TTGGAGGACA ACACTCTCAC AATGGCTGCC CTGTCTCCAG GTAAGATTAA GCCACACATG 
GCTAAGAAAT CAGTATCACG TCAGTATTCC ACTGAAGACA CCATACTGTC CTTTTTAGAC 
TCCTCTGCAG TTGAGATGGG ACCAGATAAA CTACAGTTAG AGAAAAAACG TACTTTTGAT 
GTAAAGAATC ATTGTGACCC AGAAACAGCA ACAACCTCAG CTGGTAGCAA TCTTCTAGAT 
TCAAGTGTGA CAAAAGACCA AATACAAATG GAAAGTGAGC CTATTCCAGA AAACACACCA 
AGTAAAAATT GTGACTTTCC CACAGAAGGA TTTCAGATGG TCTCTGTTGC CCACCCAAAT 
GATTTGGATG GTAAAGTATT ACAATATGGA GGTGGTCCAG ATGGAGCCGT TGCACAACTG 
CAGAAGCCAT CCTCTGCAGA TTGGGAGGGA GCCGCACACC CTGAGTCTGT GGATGTGCTC 
ACAGCTCAGT CAAACTCGCC AACCAGACCC ACAGACATAA GCAGTCAACT TAAGCATCAA 
CTTGGGATGA GCCGCCACCA TGACAAAGTG CCTGGGAATA TCAGCTATGA AGCAGAGCTA 
TCAAGTGCAA ATTCATGCCC TGAAAAATGT TCCGAGCAAG AAAATATCGC TTCAGGTATT 
TCAGCCCAAA GTGCCTCTGA TAACAGCGGA GCAGAGGAGA CCCAAGGAAG TGTGGACGAG 
GTTTCACTGA AAGAATCGTC ACCGAGTGAT GACTCTATGA TTTCGCCACT TAGTGAAGAC 
TCTCAGGCTG AAGCAGAAGG TGTGTTTGTG TCTCCAAACA AACCTCGTAC AACTGAAGAT 
TTGTTTGCAG TCATTCACAG GTCGAAGAGG AAAGTACTTG GAAGAAAAGA TTCTGGGGAT 
ATGTCTGCTC GAAGCAAATC AAGAGCTTCT CTTGGCAGCA GTAGCAGCAG CAACGCTGGC 
TCTGTCACTT TGCCCAACAG CAGCGTGACC AGCCCAAACA GCCAGAGGTC TCCTGGCCTC 
ATCTACCGAA ATGCCAAAAA GTCCAACACA TCGAATGAAG AGTTTAAGCT GTTACTCCTC 
AAGAAAGGAA GTCGCTCAGA TTCTAGTTAC CGCATGTCTG CTACTGAGAT CCTGAAAAGT 
CCCATCCTGC CCAAACCTCC TGGAGAGCTC ACAGCTGAAT CCCCACAAAG CACCCAGGAG 
GCCCATCAGG GGGCACCTGG GGCCGAGGCT TTGTCCCCAC TCTCTCCATG CTCCCCAAGA 
GTTAATGCAG AAGGGTTTTC CTCAAAGAAC TTTGCCACCT CAGCAGCAGC AAGGGTGGGA 
CGCTCCCGAG CTCCCCCTGC GGCCAGCAGC AGCCGCTACA GCGTCCGCTG CAGGCTGTAC 
AATGCACCCA TGCAGGCCAT CTCTGAGGGT GAGACAGAAA ATTCTGATGG AAGCCCACAT 
GATGACCGAT CCTCCCAGAG CTCAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967163.1
CDS	361..5310
Translation	MPFAKRIVEPQWLCRQRRPAPGPAEDANGGSADPPPPLQPPGRRDEAVAPGPEDPPRAPPAPPGPPPPPPLPAPTDQAQPPHGEAPAAGEESAAGVAEAASAAGEASSAAAAAVLLMLDLCAVSNAALARVLRQLSDVARHACSLFQELESDIQLTHRRVWALQGKLGGVQRVLGTLDPKQEAVPVSNLDIESKLSVYYRAPWHQQRNIFLPATRPPCVEELHRHARQSLQALRREHRSRSDRREQRAAAPLSVAAPPLPAYPPAHSQRRREVKDRHFLTFNSTRSPSPTECCHMTPWSRKSHPPEDEDTDVMLGQRPKNPVHNIPSTLDKQTNWSKALPLPTPEEKMKQDAQVISSCIIPINVTGVGFDREASIRCSLVHSQSVLQRRRKLRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPDPSNTVNRRSGTRDSECQTEDILIAAPSRRRIRAQRGQSIAASLSHSAGNISALADKGDTMFTTAVSSRTRSRSLPREGNRSGDAEPKVGVKPAAYEEGEPFMGDQERTPNDCSEAPSSPSAQEHQPALGLACSQHLHSPQHKLNERGRSRLSRMAADSGSCDISSNSDTFGSPIHCISTAGVLLSSHMDQKDDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNTAPHANEDANVFVTEQYNDHLDKVRGHRANSFTSTVADLLDDPNNSNTSDSEWNYLHHHHDASCRQDFSPERPKADSLGCPSFTSMATYDSFLEKSPSDKADTSSHFSVDTEGYYTSMHFDCGLKGNKSYVCHYAALGPENSQGIGVPPTLPDCTWQDYLDHRRQGRPSISFRKPKAKPTPPKRSSSLRKSDGNADISEKKEPKISSGQLLPHSSREMKLPLDFSNTPSLMENANLAIKQESSWMNQSEHGIKEPQLDTSDIPPFKDEGAESTHYADLWLLNDLKTNDPYRSLSNSSTATGTTVIECIKSPESSESQTSQSESRATTPSLPSVDNEFKLASPEKLAGLASPSSGYSSQSETPTSSFPTAFFSGPLSPGGSKRKPKVPERKSSLQQPSLKDGALSLSKDLELPIIPPTHLDLSALHSVLNKPFHHRHPLHVFTHSKQNPVGETLRSNPPPSLAITPTVLKSVNLRSISKSEEVKPKEGNNTDLPYLEDNTLTMAALSPGKIKPHMAKKSVSRQYSTEDTILSFLDSSAVEMGPDKLQLEKKRTFDVKNHCDPETATTSAGSNLLDSSVTKDQIQMESEPIPENTPSKNCDFPTEGFQMVSVAHPNDLDGKVLQYGGGPDGAVAQLQKPSSADWEGAAHPESVDVLTAQSNSPTRPTDISSQLKHQLGMSRHHDKVPGNISYEAELSSANSCPEKCSEQENIASGISAQSASDNSGAEETQGSVDEVSLKESSPSDDSMISPLSEDSQAEAEGVFVSPNKPRTTEDLFAVIHRSKRKVLGRKDSGDMSARSKSRASLGSSSSSNAGSVTLPNSSVTSPNSQRSPGLIYRNAKKSNTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPILPKPPGELTAESPQSTQEAHQGAPGAEALSPLSPCSPRVNAEGFSSKNFATSAAARVGRSRAPPAASSSRYSVRCRLYNAPMQAISEGETENSDGSPHDDRSSQSST

Target ORF information:

RefSeq Version	XM_014111688.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris Nance-Horan syndrome (congenital cataracts and dental anomalies) (NHS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111688.1

ORF Insert Sequence:

ATGCCTTTCG CCAAGAGGAT CGTGGAGCCG CAGTGGCTGT GCCGGCAGCG GCGCCCGGCG 
CCCGGCCCGG CGGAGGACGC GAACGGAGGC AGCGCTGACC CGCCGCCGCC CCTGCAGCCG 
CCCGGCCGAC GGGACGAGGC CGTGGCGCCG GGGCCGGAGG ATCCTCCCCG CGCGCCCCCC 
GCGCCTCCCG GGCCGCCGCC GCCGCCGCCG CTGCCCGCGC CGACCGACCA GGCGCAGCCG 
CCGCACGGAG AGGCGCCAGC GGCGGGCGAG GAGAGCGCGG CGGGGGTCGC CGAGGCGGCG 
TCCGCGGCCG GCGAGGCGTC CTCGGCGGCG GCGGCGGCCG TGCTGCTCAT GCTGGACCTG 
TGCGCGGTCA GCAACGCCGC GCTGGCCCGC GTCCTCCGGC AGCTCTCGGA CGTGGCCCGG 
CACGCGTGCA GCCTCTTCCA GGAGCTCGAG AGCGACATCC AGCTGACCCA CCGCCGCGTC 
TGGGCGCTGC AGGGCAAGCT CGGCGGCGTG CAGCGCGTCC TCGGCACGCT GGACCCCAAG 
CAGGAGGCAG TGCCTGTATC CAACCTGGAC ATTGAGAGTA AGCTGAGTGT ATACTACCGT 
GCACCATGGC ACCAGCAGAG AAACATCTTC CTCCCAGCCA CAAGGCCACC CTGCGTGGAG 
GAACTGCATC GCCATGCCCG GCAGAGCCTG CAAGCCCTGC GCAGAGAACA CCGGAGCCGA 
AGTGATCGCC GGGAGCAGAG AGCCGCTGCC CCCCTTTCTG TAGCAGCTCC TCCACTGCCA 
GCCTATCCAC CAGCTCACAG CCAGAGGAGG CGTGAAGTTA AGGACCGGCA CTTCTTAACG 
TTTAACAGCA CCCGTTCGCC CTCCCCCACT GAGTGTTGCC ACATGACCCC ATGGAGTAGA 
AAGTCCCATC CCCCGGAGGA TGAAGATACA GATGTCATGT TAGGGCAGAG GCCGAAAAAC 
CCAGTACACA ATATCCCTTC AACACTGGAC AAGCAGACCA ACTGGAGCAA AGCACTGCCT 
CTCCCAACAC CAGAGGAGAA AATGAAACAA GATGCCCAAG TGATTTCTTC TTGCATTATT 
CCCATCAATG TCACTGGAGT TGGTTTTGAC AGAGAGGCTA GTATACGCTG CTCTCTTGTT 
CATTCACAAT CCGTACTACA GCGGAGACGA AAACTGAGGA GGAGGAAAAC CATCTCGGGT 
ATCCCCAGAA GAGTCCAACA AGAAATAGAT TCTGATGAAT CCCCTGTGGC CAGGGAAAGG 
AATGTGATTG TGCACACAAA TCCAGACCCT TCCAACACTG TCAATAGGAG GTCTGGAACC 
AGGGACTCAG AGTGCCAAAC CGAAGATATC CTGATTGCTG CTCCATCCAG AAGGAGAATC 
AGAGCTCAAA GGGGTCAAAG CATAGCAGCT TCCCTTTCTC ATTCTGCTGG CAACATCTCT 
GCATTGGCTG ACAAAGGTGA CACCATGTTT ACAACTGCAG TGAGCAGCCG TACAAGATCT 
CGGAGCCTTC CCCGGGAGGG CAACAGAAGT GGGGATGCTG AGCCCAAAGT TGGTGTTAAA 
CCCGCAGCAT ATGAAGAGGG GGAGCCTTTC ATGGGCGACC AAGAAAGAAC CCCTAATGAT 
TGCAGTGAGG CCCCCAGCAG CCCAAGTGCA CAGGAACACC AGCCTGCTTT GGGTCTGGCT 
TGTTCTCAAC ATCTTCACAG CCCCCAGCAC AAGTTAAATG AGAGAGGGAG GTCACGTCTG 
TCCCGAATGG CTGCTGACTC TGGCAGTTGT GACATCTCCT CCAACTCAGA CACCTTTGGG 
AGCCCCATCC ACTGCATCTC CACGGCAGGT GTCCTCCTCA GCAGCCACAT GGACCAGAAA 
GATGACCATC AGTCATCCAG TGGCAACTGG AGTGGGAGCA GCTCCACATG CCCCTCACAG 
ACCTCAGAGA CAATCCCTCC TGCAGCTTCT CCTCCCCTCA CTGGCTCTTC ACACTGTGAC 
TCAGAGTTGT CACTAAACAC AGCCCCTCAT GCTAATGAGG ATGCCAATGT CTTCGTGACA 
GAGCAGTACA ATGACCACTT GGATAAAGTG AGAGGCCACC GGGCAAACTC TTTTACCTCC 
ACTGTGGCGG ATCTGCTGGA TGACCCCAAC AATAGCAACA CCAGTGACAG TGAGTGGAAT 
TACCTACACC ACCATCACGA TGCCTCCTGC CGCCAGGATT TTAGTCCTGA GCGCCCTAAG 
GCAGACAGCC TGGGCTGCCC GAGCTTCACA AGCATGGCCA CTTACGACAG CTTTCTGGAA 
AAGTCTCCAT CAGACAAAGC AGACACTAGC TCTCACTTTT CAGTGGACAC AGAAGGATAC 
TACACTTCCA TGCACTTTGA CTGTGGTCTT AAAGGTAATA AGAGCTATGT GTGTCACTAT 
GCAGCCCTGG GCCCAGAGAA TAGCCAGGGC ATTGGGGTTC CTCCGACTCT TCCGGACTGT 
ACCTGGCAGG ACTACTTAGA CCACAGGAGG CAGGGGAGAC CAAGCATCTC TTTCAGGAAA 
CCAAAGGCAA AGCCGACCCC ACCTAAACGT AGCTCATCTT TGAGGAAGTC CGATGGAAAT 
GCAGACATTT CTGAGAAGAA AGAACCAAAG ATAAGCAGTG GCCAGCTCCT GCCTCACAGT 
TCCAGGGAAA TGAAGCTGCC TCTTGATTTC TCCAACACGC CTTCTCTAAT GGAAAATGCC 
AACCTTGCCA TCAAGCAAGA GTCTTCTTGG ATGAACCAGA GTGAACATGG TATCAAGGAA 
CCTCAGTTAG ACACTTCAGA TATTCCGCCA TTCAAAGATG AAGGTGCTGA ATCGACTCAC 
TATGCAGATC TCTGGCTTCT AAATGACTTG AAAACAAATG ATCCTTACAG ATCTTTATCC 
AATTCAAGCA CTGCTACGGG TACCACAGTT ATTGAATGCA TCAAGTCTCC AGAGAGCTCT 
GAATCCCAAA CATCCCAGTC AGAATCAAGA GCCACCACCC CATCCCTTCC TTCTGTTGAC 
AATGAGTTTA AACTGGCTTC ACCAGAAAAG CTGGCAGGCT TGGCATCTCC ATCAAGTGGC 
TACTCGAGCC AGTCTGAAAC ACCGACCTCC TCTTTCCCTA CAGCTTTCTT TTCTGGCCCA 
CTGTCTCCTG GAGGTAGCAA AAGAAAACCG AAAGTTCCAG AAAGAAAATC CTCACTACAG 
CAGCCCTCTT TAAAAGATGG AGCTCTATCA CTGAGTAAAG ACCTTGAACT TCCAATTATA 
CCTCCTACCC ATCTTGACCT AAGTGCTCTT CATAGTGTCT TGAATAAACC ATTCCATCAC 
CGTCACCCAT TGCATGTTTT TACTCATAGT AAACAGAACC CAGTAGGAGA AACACTGCGG 
TCAAATCCTC CACCATCCCT TGCAATCACA CCAACGGTCC TGAAATCTGT TAACCTGAGG 
TCCATCAGTA AATCTGAAGA AGTTAAGCCA AAAGAGGGCA ACAATACAGA TCTCCCCTAC 
TTGGAGGACA ACACTCTCAC AATGGCTGCC CTGTCTCCAG GTAAGATTAA GCCACACATG 
GCTAAGAAAT CAGTATCACG TCAGTATTCC ACTGAAGACA CCATACTGTC CTTTTTAGAC 
TCCTCTGCAG TTGAGATGGG ACCAGATAAA CTACAGTTAG AGAAAAAACG TACTTTTGAT 
GTAAAGAATC ATTGTGACCC AGAAACAGCA ACAACCTCAG CTGGTAGCAA TCTTCTAGAT 
TCAAGTGTGA CAAAAGACCA AATACAAATG GAAAGTGAGC CTATTCCAGA AAACACACCA 
AGTAAAAATT GTGACTTTCC CACAGAAGGA TTTCAGATGG TCTCTGTTGC CCACCCAAAT 
GATTTGGATG GTAAAGTATT ACAATATGGA GGTGGTCCAG ATGGAGCCGT TGCACAACTG 
CAGAAGCCAT CCTCTGCAGA TTGGGAGGGA GCCGCACACC CTGAGTCTGT GGATGTGCTC 
ACAGCTCAGT CAAACTCGCC AACCAGACCC ACAGACATAA GCAGTCAACT TAAGCATCAA 
CTTGGGATGA GCCGCCACCA TGACAAAGTG CCTGGGAATA TCAGCTATGA AGCAGAGCTA 
TCAAGTGCAA ATTCATGCCC TGAAAAATGT TCCGAGCAAG AAAATATCGC TTCAGGTATT 
TCAGCCCAAA GTGCCTCTGA TAACAGCGGA GCAGAGGAGA CCCAAGGAAG TGTGGACGAG 
GTTTCACTGA AAGAATCGTC ACCGAGTGAT GACTCTATGA TTTCGCCACT TAGTGAAGAC 
TCTCAGGCTG AAGCAGAAGG TGTGTTTGTG TCTCCAAACA AACCTCGTAC AACTGAAGAT 
TTGTTTGCAG TCATTCACAG GTCGAAGAGG AAAGTACTTG GAAGAAAAGA TTCTGGGGAT 
ATGTCTGCTC GAAGCAAATC AAGAGCTTCT CTTGGCAGCA GTAGCAGCAG CAACGCTGGC 
TCTGTCACTT TGCCCAACAG CAGCGTGACC AGCCCAAACA GCCAGAGGTC TCCTGGCCTC 
ATCTACCGAA ATGCCAAAAA GTCCAACACA TCGAATGAAG AGTTTAAGCT GTTACTCCTC 
AAGAAAGGAA GTCGCTCAGA TTCTAGTTAC CGCATGTCTG CTACTGAGAT CCTGAAAAGT 
CCCATCCTGC CCAAACCTCC TGGAGAGCTC ACAGCTGAAT CCCCACAAAG CACCCAGGAG 
GCCCATCAGG GGGCACCTGG GGCCGAGGCT TTGTCCCCAC TCTCTCCATG CTCCCCAAGA 
GTTAATGCAG AAGGGTTTTC CTCAAAGAAC TTTGCCACCT CAGCAGCAGC AAGGGTGGGA 
CGCTCCCGAG CTCCCCCTGC GGCCAGCAGC AGCCGCTACA GCGTCCGCTG CAGGCTGTAC 
AATGCACCCA TGCAGGCCAT CTCTGAGGGT GAGACAGAAA ATTCTGATGG AAGCCCACAT 
GATGACCGAT CCTCCCAGAG CTCAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NHS ( XM_014111688.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013967163.1 Canis lupus familiaris NHS actin remodeling regulator (NHS), transcript variant X1, mRNA.

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CloneID	OCe45625
Clone ID Related Accession (Same CDS sequence)	XM_014111688.1 , XM_014111688.2
Accession Version	XM_014111688.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	Nance-Horan syndrome protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTCG CCAAGAGGAT CGTGGAGCCG CAGTGGCTGT GCCGGCAGCG GCGCCCGGCG 
CCCGGCCCGG CGGAGGACGC GAACGGAGGC AGCGCTGACC CGCCGCCGCC CCTGCAGCCG 
CCCGGCCGAC GGGACGAGGC CGTGGCGCCG GGGCCGGAGG ATCCTCCCCG CGCGCCCCCC 
GCGCCTCCCG GGCCGCCGCC GCCGCCGCCG CTGCCCGCGC CGACCGACCA GGCGCAGCCG 
CCGCACGGAG AGGCGCCAGC GGCGGGCGAG GAGAGCGCGG CGGGGGTCGC CGAGGCGGCG 
TCCGCGGCCG GCGAGGCGTC CTCGGCGGCG GCGGCGGCCG TGCTGCTCAT GCTGGACCTG 
TGCGCGGTCA GCAACGCCGC GCTGGCCCGC GTCCTCCGGC AGCTCTCGGA CGTGGCCCGG 
CACGCGTGCA GCCTCTTCCA GGAGCTCGAG AGCGACATCC AGCTGACCCA CCGCCGCGTC 
TGGGCGCTGC AGGGCAAGCT CGGCGGCGTG CAGCGCGTCC TCGGCACGCT GGACCCCAAG 
CAGGAGGCAG TGCCTGTATC CAACCTGGAC ATTGAGAGTA AGCTGAGTGT ATACTACCGT 
GCACCATGGC ACCAGCAGAG AAACATCTTC CTCCCAGCCA CAAGGCCACC CTGCGTGGAG 
GAACTGCATC GCCATGCCCG GCAGAGCCTG CAAGCCCTGC GCAGAGAACA CCGGAGCCGA 
AGTGATCGCC GGGAGCAGAG AGCCGCTGCC CCCCTTTCTG TAGCAGCTCC TCCACTGCCA 
GCCTATCCAC CAGCTCACAG CCAGAGGAGG CGTGAAGTTA AGGACCGGCA CTTCTTAACG 
TTTAACAGCA CCCGTTCGCC CTCCCCCACT GAGTGTTGCC ACATGACCCC ATGGAGTAGA 
AAGTCCCATC CCCCGGAGGA TGAAGATACA GATGTCATGT TAGGGCAGAG GCCGAAAAAC 
CCAGTACACA ATATCCCTTC AACACTGGAC AAGCAGACCA ACTGGAGCAA AGCACTGCCT 
CTCCCAACAC CAGAGGAGAA AATGAAACAA GATGCCCAAG TGATTTCTTC TTGCATTATT 
CCCATCAATG TCACTGGAGT TGGTTTTGAC AGAGAGGCTA GTATACGCTG CTCTCTTGTT 
CATTCACAAT CCGTACTACA GCGGAGACGA AAACTGAGGA GGAGGAAAAC CATCTCGGGT 
ATCCCCAGAA GAGTCCAACA AGAAATAGAT TCTGATGAAT CCCCTGTGGC CAGGGAAAGG 
AATGTGATTG TGCACACAAA TCCAGACCCT TCCAACACTG TCAATAGGAG GTCTGGAACC 
AGGGACTCAG AGTGCCAAAC CGAAGATATC CTGATTGCTG CTCCATCCAG AAGGAGAATC 
AGAGCTCAAA GGGGTCAAAG CATAGCAGCT TCCCTTTCTC ATTCTGCTGG CAACATCTCT 
GCATTGGCTG ACAAAGGTGA CACCATGTTT ACAACTGCAG TGAGCAGCCG TACAAGATCT 
CGGAGCCTTC CCCGGGAGGG CAACAGAAGT GGGGATGCTG AGCCCAAAGT TGGTGTTAAA 
CCCGCAGCAT ATGAAGAGGG GGAGCCTTTC ATGGGCGACC AAGAAAGAAC CCCTAATGAT 
TGCAGTGAGG CCCCCAGCAG CCCAAGTGCA CAGGAACACC AGCCTGCTTT GGGTCTGGCT 
TGTTCTCAAC ATCTTCACAG CCCCCAGCAC AAGTTAAATG AGAGAGGGAG GTCACGTCTG 
TCCCGAATGG CTGCTGACTC TGGCAGTTGT GACATCTCCT CCAACTCAGA CACCTTTGGG 
AGCCCCATCC ACTGCATCTC CACGGCAGGT GTCCTCCTCA GCAGCCACAT GGACCAGAAA 
GATGACCATC AGTCATCCAG TGGCAACTGG AGTGGGAGCA GCTCCACATG CCCCTCACAG 
ACCTCAGAGA CAATCCCTCC TGCAGCTTCT CCTCCCCTCA CTGGCTCTTC ACACTGTGAC 
TCAGAGTTGT CACTAAACAC AGCCCCTCAT GCTAATGAGG ATGCCAATGT CTTCGTGACA 
GAGCAGTACA ATGACCACTT GGATAAAGTG AGAGGCCACC GGGCAAACTC TTTTACCTCC 
ACTGTGGCGG ATCTGCTGGA TGACCCCAAC AATAGCAACA CCAGTGACAG TGAGTGGAAT 
TACCTACACC ACCATCACGA TGCCTCCTGC CGCCAGGATT TTAGTCCTGA GCGCCCTAAG 
GCAGACAGCC TGGGCTGCCC GAGCTTCACA AGCATGGCCA CTTACGACAG CTTTCTGGAA 
AAGTCTCCAT CAGACAAAGC AGACACTAGC TCTCACTTTT CAGTGGACAC AGAAGGATAC 
TACACTTCCA TGCACTTTGA CTGTGGTCTT AAAGGTAATA AGAGCTATGT GTGTCACTAT 
GCAGCCCTGG GCCCAGAGAA TAGCCAGGGC ATTGGGGTTC CTCCGACTCT TCCGGACTGT 
ACCTGGCAGG ACTACTTAGA CCACAGGAGG CAGGGGAGAC CAAGCATCTC TTTCAGGAAA 
CCAAAGGCAA AGCCGACCCC ACCTAAACGT AGCTCATCTT TGAGGAAGTC CGATGGAAAT 
GCAGACATTT CTGAGAAGAA AGAACCAAAG ATAAGCAGTG GCCAGCTCCT GCCTCACAGT 
TCCAGGGAAA TGAAGCTGCC TCTTGATTTC TCCAACACGC CTTCTCTAAT GGAAAATGCC 
AACCTTGCCA TCAAGCAAGA GTCTTCTTGG ATGAACCAGA GTGAACATGG TATCAAGGAA 
CCTCAGTTAG ACACTTCAGA TATTCCGCCA TTCAAAGATG AAGGTGCTGA ATCGACTCAC 
TATGCAGATC TCTGGCTTCT AAATGACTTG AAAACAAATG ATCCTTACAG ATCTTTATCC 
AATTCAAGCA CTGCTACGGG TACCACAGTT ATTGAATGCA TCAAGTCTCC AGAGAGCTCT 
GAATCCCAAA CATCCCAGTC AGAATCAAGA GCCACCACCC CATCCCTTCC TTCTGTTGAC 
AATGAGTTTA AACTGGCTTC ACCAGAAAAG CTGGCAGGCT TGGCATCTCC ATCAAGTGGC 
TACTCGAGCC AGTCTGAAAC ACCGACCTCC TCTTTCCCTA CAGCTTTCTT TTCTGGCCCA 
CTGTCTCCTG GAGGTAGCAA AAGAAAACCG AAAGTTCCAG AAAGAAAATC CTCACTACAG 
CAGCCCTCTT TAAAAGATGG AGCTCTATCA CTGAGTAAAG ACCTTGAACT TCCAATTATA 
CCTCCTACCC ATCTTGACCT AAGTGCTCTT CATAGTGTCT TGAATAAACC ATTCCATCAC 
CGTCACCCAT TGCATGTTTT TACTCATAGT AAACAGAACC CAGTAGGAGA AACACTGCGG 
TCAAATCCTC CACCATCCCT TGCAATCACA CCAACGGTCC TGAAATCTGT TAACCTGAGG 
TCCATCAGTA AATCTGAAGA AGTTAAGCCA AAAGAGGGCA ACAATACAGA TCTCCCCTAC 
TTGGAGGACA ACACTCTCAC AATGGCTGCC CTGTCTCCAG GTAAGATTAA GCCACACATG 
GCTAAGAAAT CAGTATCACG TCAGTATTCC ACTGAAGACA CCATACTGTC CTTTTTAGAC 
TCCTCTGCAG TTGAGATGGG ACCAGATAAA CTACAGTTAG AGAAAAAACG TACTTTTGAT 
GTAAAGAATC ATTGTGACCC AGAAACAGCA ACAACCTCAG CTGGTAGCAA TCTTCTAGAT 
TCAAGTGTGA CAAAAGACCA AATACAAATG GAAAGTGAGC CTATTCCAGA AAACACACCA 
AGTAAAAATT GTGACTTTCC CACAGAAGGA TTTCAGATGG TCTCTGTTGC CCACCCAAAT 
GATTTGGATG GTAAAGTATT ACAATATGGA GGTGGTCCAG ATGGAGCCGT TGCACAACTG 
CAGAAGCCAT CCTCTGCAGA TTGGGAGGGA GCCGCACACC CTGAGTCTGT GGATGTGCTC 
ACAGCTCAGT CAAACTCGCC AACCAGACCC ACAGACATAA GCAGTCAACT TAAGCATCAA 
CTTGGGATGA GCCGCCACCA TGACAAAGTG CCTGGGAATA TCAGCTATGA AGCAGAGCTA 
TCAAGTGCAA ATTCATGCCC TGAAAAATGT TCCGAGCAAG AAAATATCGC TTCAGGTATT 
TCAGCCCAAA GTGCCTCTGA TAACAGCGGA GCAGAGGAGA CCCAAGGAAG TGTGGACGAG 
GTTTCACTGA AAGAATCGTC ACCGAGTGAT GACTCTATGA TTTCGCCACT TAGTGAAGAC 
TCTCAGGCTG AAGCAGAAGG TGTGTTTGTG TCTCCAAACA AACCTCGTAC AACTGAAGAT 
TTGTTTGCAG TCATTCACAG GTCGAAGAGG AAAGTACTTG GAAGAAAAGA TTCTGGGGAT 
ATGTCTGCTC GAAGCAAATC AAGAGCTTCT CTTGGCAGCA GTAGCAGCAG CAACGCTGGC 
TCTGTCACTT TGCCCAACAG CAGCGTGACC AGCCCAAACA GCCAGAGGTC TCCTGGCCTC 
ATCTACCGAA ATGCCAAAAA GTCCAACACA TCGAATGAAG AGTTTAAGCT GTTACTCCTC 
AAGAAAGGAA GTCGCTCAGA TTCTAGTTAC CGCATGTCTG CTACTGAGAT CCTGAAAAGT 
CCCATCCTGC CCAAACCTCC TGGAGAGCTC ACAGCTGAAT CCCCACAAAG CACCCAGGAG 
GCCCATCAGG GGGCACCTGG GGCCGAGGCT TTGTCCCCAC TCTCTCCATG CTCCCCAAGA 
GTTAATGCAG AAGGGTTTTC CTCAAAGAAC TTTGCCACCT CAGCAGCAGC AAGGGTGGGA 
CGCTCCCGAG CTCCCCCTGC GGCCAGCAGC AGCCGCTACA GCGTCCGCTG CAGGCTGTAC 
AATGCACCCA TGCAGGCCAT CTCTGAGGGT GAGACAGAAA ATTCTGATGG AAGCCCACAT 
GATGACCGAT CCTCCCAGAG CTCAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013967163.1
CDS	597..5546
Translation	MPFAKRIVEPQWLCRQRRPAPGPAEDANGGSADPPPPLQPPGRRDEAVAPGPEDPPRAPPAPPGPPPPPPLPAPTDQAQPPHGEAPAAGEESAAGVAEAASAAGEASSAAAAAVLLMLDLCAVSNAALARVLRQLSDVARHACSLFQELESDIQLTHRRVWALQGKLGGVQRVLGTLDPKQEAVPVSNLDIESKLSVYYRAPWHQQRNIFLPATRPPCVEELHRHARQSLQALRREHRSRSDRREQRAAAPLSVAAPPLPAYPPAHSQRRREVKDRHFLTFNSTRSPSPTECCHMTPWSRKSHPPEDEDTDVMLGQRPKNPVHNIPSTLDKQTNWSKALPLPTPEEKMKQDAQVISSCIIPINVTGVGFDREASIRCSLVHSQSVLQRRRKLRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPDPSNTVNRRSGTRDSECQTEDILIAAPSRRRIRAQRGQSIAASLSHSAGNISALADKGDTMFTTAVSSRTRSRSLPREGNRSGDAEPKVGVKPAAYEEGEPFMGDQERTPNDCSEAPSSPSAQEHQPALGLACSQHLHSPQHKLNERGRSRLSRMAADSGSCDISSNSDTFGSPIHCISTAGVLLSSHMDQKDDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNTAPHANEDANVFVTEQYNDHLDKVRGHRANSFTSTVADLLDDPNNSNTSDSEWNYLHHHHDASCRQDFSPERPKADSLGCPSFTSMATYDSFLEKSPSDKADTSSHFSVDTEGYYTSMHFDCGLKGNKSYVCHYAALGPENSQGIGVPPTLPDCTWQDYLDHRRQGRPSISFRKPKAKPTPPKRSSSLRKSDGNADISEKKEPKISSGQLLPHSSREMKLPLDFSNTPSLMENANLAIKQESSWMNQSEHGIKEPQLDTSDIPPFKDEGAESTHYADLWLLNDLKTNDPYRSLSNSSTATGTTVIECIKSPESSESQTSQSESRATTPSLPSVDNEFKLASPEKLAGLASPSSGYSSQSETPTSSFPTAFFSGPLSPGGSKRKPKVPERKSSLQQPSLKDGALSLSKDLELPIIPPTHLDLSALHSVLNKPFHHRHPLHVFTHSKQNPVGETLRSNPPPSLAITPTVLKSVNLRSISKSEEVKPKEGNNTDLPYLEDNTLTMAALSPGKIKPHMAKKSVSRQYSTEDTILSFLDSSAVEMGPDKLQLEKKRTFDVKNHCDPETATTSAGSNLLDSSVTKDQIQMESEPIPENTPSKNCDFPTEGFQMVSVAHPNDLDGKVLQYGGGPDGAVAQLQKPSSADWEGAAHPESVDVLTAQSNSPTRPTDISSQLKHQLGMSRHHDKVPGNISYEAELSSANSCPEKCSEQENIASGISAQSASDNSGAEETQGSVDEVSLKESSPSDDSMISPLSEDSQAEAEGVFVSPNKPRTTEDLFAVIHRSKRKVLGRKDSGDMSARSKSRASLGSSSSSNAGSVTLPNSSVTSPNSQRSPGLIYRNAKKSNTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPILPKPPGELTAESPQSTQEAHQGAPGAEALSPLSPCSPRVNAEGFSSKNFATSAAARVGRSRAPPAASSSRYSVRCRLYNAPMQAISEGETENSDGSPHDDRSSQSST

Target ORF information:

RefSeq Version	XM_014111688.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris NHS actin remodeling regulator (NHS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111688.2

ORF Insert Sequence:

ATGCCTTTCG CCAAGAGGAT CGTGGAGCCG CAGTGGCTGT GCCGGCAGCG GCGCCCGGCG 
CCCGGCCCGG CGGAGGACGC GAACGGAGGC AGCGCTGACC CGCCGCCGCC CCTGCAGCCG 
CCCGGCCGAC GGGACGAGGC CGTGGCGCCG GGGCCGGAGG ATCCTCCCCG CGCGCCCCCC 
GCGCCTCCCG GGCCGCCGCC GCCGCCGCCG CTGCCCGCGC CGACCGACCA GGCGCAGCCG 
CCGCACGGAG AGGCGCCAGC GGCGGGCGAG GAGAGCGCGG CGGGGGTCGC CGAGGCGGCG 
TCCGCGGCCG GCGAGGCGTC CTCGGCGGCG GCGGCGGCCG TGCTGCTCAT GCTGGACCTG 
TGCGCGGTCA GCAACGCCGC GCTGGCCCGC GTCCTCCGGC AGCTCTCGGA CGTGGCCCGG 
CACGCGTGCA GCCTCTTCCA GGAGCTCGAG AGCGACATCC AGCTGACCCA CCGCCGCGTC 
TGGGCGCTGC AGGGCAAGCT CGGCGGCGTG CAGCGCGTCC TCGGCACGCT GGACCCCAAG 
CAGGAGGCAG TGCCTGTATC CAACCTGGAC ATTGAGAGTA AGCTGAGTGT ATACTACCGT 
GCACCATGGC ACCAGCAGAG AAACATCTTC CTCCCAGCCA CAAGGCCACC CTGCGTGGAG 
GAACTGCATC GCCATGCCCG GCAGAGCCTG CAAGCCCTGC GCAGAGAACA CCGGAGCCGA 
AGTGATCGCC GGGAGCAGAG AGCCGCTGCC CCCCTTTCTG TAGCAGCTCC TCCACTGCCA 
GCCTATCCAC CAGCTCACAG CCAGAGGAGG CGTGAAGTTA AGGACCGGCA CTTCTTAACG 
TTTAACAGCA CCCGTTCGCC CTCCCCCACT GAGTGTTGCC ACATGACCCC ATGGAGTAGA 
AAGTCCCATC CCCCGGAGGA TGAAGATACA GATGTCATGT TAGGGCAGAG GCCGAAAAAC 
CCAGTACACA ATATCCCTTC AACACTGGAC AAGCAGACCA ACTGGAGCAA AGCACTGCCT 
CTCCCAACAC CAGAGGAGAA AATGAAACAA GATGCCCAAG TGATTTCTTC TTGCATTATT 
CCCATCAATG TCACTGGAGT TGGTTTTGAC AGAGAGGCTA GTATACGCTG CTCTCTTGTT 
CATTCACAAT CCGTACTACA GCGGAGACGA AAACTGAGGA GGAGGAAAAC CATCTCGGGT 
ATCCCCAGAA GAGTCCAACA AGAAATAGAT TCTGATGAAT CCCCTGTGGC CAGGGAAAGG 
AATGTGATTG TGCACACAAA TCCAGACCCT TCCAACACTG TCAATAGGAG GTCTGGAACC 
AGGGACTCAG AGTGCCAAAC CGAAGATATC CTGATTGCTG CTCCATCCAG AAGGAGAATC 
AGAGCTCAAA GGGGTCAAAG CATAGCAGCT TCCCTTTCTC ATTCTGCTGG CAACATCTCT 
GCATTGGCTG ACAAAGGTGA CACCATGTTT ACAACTGCAG TGAGCAGCCG TACAAGATCT 
CGGAGCCTTC CCCGGGAGGG CAACAGAAGT GGGGATGCTG AGCCCAAAGT TGGTGTTAAA 
CCCGCAGCAT ATGAAGAGGG GGAGCCTTTC ATGGGCGACC AAGAAAGAAC CCCTAATGAT 
TGCAGTGAGG CCCCCAGCAG CCCAAGTGCA CAGGAACACC AGCCTGCTTT GGGTCTGGCT 
TGTTCTCAAC ATCTTCACAG CCCCCAGCAC AAGTTAAATG AGAGAGGGAG GTCACGTCTG 
TCCCGAATGG CTGCTGACTC TGGCAGTTGT GACATCTCCT CCAACTCAGA CACCTTTGGG 
AGCCCCATCC ACTGCATCTC CACGGCAGGT GTCCTCCTCA GCAGCCACAT GGACCAGAAA 
GATGACCATC AGTCATCCAG TGGCAACTGG AGTGGGAGCA GCTCCACATG CCCCTCACAG 
ACCTCAGAGA CAATCCCTCC TGCAGCTTCT CCTCCCCTCA CTGGCTCTTC ACACTGTGAC 
TCAGAGTTGT CACTAAACAC AGCCCCTCAT GCTAATGAGG ATGCCAATGT CTTCGTGACA 
GAGCAGTACA ATGACCACTT GGATAAAGTG AGAGGCCACC GGGCAAACTC TTTTACCTCC 
ACTGTGGCGG ATCTGCTGGA TGACCCCAAC AATAGCAACA CCAGTGACAG TGAGTGGAAT 
TACCTACACC ACCATCACGA TGCCTCCTGC CGCCAGGATT TTAGTCCTGA GCGCCCTAAG 
GCAGACAGCC TGGGCTGCCC GAGCTTCACA AGCATGGCCA CTTACGACAG CTTTCTGGAA 
AAGTCTCCAT CAGACAAAGC AGACACTAGC TCTCACTTTT CAGTGGACAC AGAAGGATAC 
TACACTTCCA TGCACTTTGA CTGTGGTCTT AAAGGTAATA AGAGCTATGT GTGTCACTAT 
GCAGCCCTGG GCCCAGAGAA TAGCCAGGGC ATTGGGGTTC CTCCGACTCT TCCGGACTGT 
ACCTGGCAGG ACTACTTAGA CCACAGGAGG CAGGGGAGAC CAAGCATCTC TTTCAGGAAA 
CCAAAGGCAA AGCCGACCCC ACCTAAACGT AGCTCATCTT TGAGGAAGTC CGATGGAAAT 
GCAGACATTT CTGAGAAGAA AGAACCAAAG ATAAGCAGTG GCCAGCTCCT GCCTCACAGT 
TCCAGGGAAA TGAAGCTGCC TCTTGATTTC TCCAACACGC CTTCTCTAAT GGAAAATGCC 
AACCTTGCCA TCAAGCAAGA GTCTTCTTGG ATGAACCAGA GTGAACATGG TATCAAGGAA 
CCTCAGTTAG ACACTTCAGA TATTCCGCCA TTCAAAGATG AAGGTGCTGA ATCGACTCAC 
TATGCAGATC TCTGGCTTCT AAATGACTTG AAAACAAATG ATCCTTACAG ATCTTTATCC 
AATTCAAGCA CTGCTACGGG TACCACAGTT ATTGAATGCA TCAAGTCTCC AGAGAGCTCT 
GAATCCCAAA CATCCCAGTC AGAATCAAGA GCCACCACCC CATCCCTTCC TTCTGTTGAC 
AATGAGTTTA AACTGGCTTC ACCAGAAAAG CTGGCAGGCT TGGCATCTCC ATCAAGTGGC 
TACTCGAGCC AGTCTGAAAC ACCGACCTCC TCTTTCCCTA CAGCTTTCTT TTCTGGCCCA 
CTGTCTCCTG GAGGTAGCAA AAGAAAACCG AAAGTTCCAG AAAGAAAATC CTCACTACAG 
CAGCCCTCTT TAAAAGATGG AGCTCTATCA CTGAGTAAAG ACCTTGAACT TCCAATTATA 
CCTCCTACCC ATCTTGACCT AAGTGCTCTT CATAGTGTCT TGAATAAACC ATTCCATCAC 
CGTCACCCAT TGCATGTTTT TACTCATAGT AAACAGAACC CAGTAGGAGA AACACTGCGG 
TCAAATCCTC CACCATCCCT TGCAATCACA CCAACGGTCC TGAAATCTGT TAACCTGAGG 
TCCATCAGTA AATCTGAAGA AGTTAAGCCA AAAGAGGGCA ACAATACAGA TCTCCCCTAC 
TTGGAGGACA ACACTCTCAC AATGGCTGCC CTGTCTCCAG GTAAGATTAA GCCACACATG 
GCTAAGAAAT CAGTATCACG TCAGTATTCC ACTGAAGACA CCATACTGTC CTTTTTAGAC 
TCCTCTGCAG TTGAGATGGG ACCAGATAAA CTACAGTTAG AGAAAAAACG TACTTTTGAT 
GTAAAGAATC ATTGTGACCC AGAAACAGCA ACAACCTCAG CTGGTAGCAA TCTTCTAGAT 
TCAAGTGTGA CAAAAGACCA AATACAAATG GAAAGTGAGC CTATTCCAGA AAACACACCA 
AGTAAAAATT GTGACTTTCC CACAGAAGGA TTTCAGATGG TCTCTGTTGC CCACCCAAAT 
GATTTGGATG GTAAAGTATT ACAATATGGA GGTGGTCCAG ATGGAGCCGT TGCACAACTG 
CAGAAGCCAT CCTCTGCAGA TTGGGAGGGA GCCGCACACC CTGAGTCTGT GGATGTGCTC 
ACAGCTCAGT CAAACTCGCC AACCAGACCC ACAGACATAA GCAGTCAACT TAAGCATCAA 
CTTGGGATGA GCCGCCACCA TGACAAAGTG CCTGGGAATA TCAGCTATGA AGCAGAGCTA 
TCAAGTGCAA ATTCATGCCC TGAAAAATGT TCCGAGCAAG AAAATATCGC TTCAGGTATT 
TCAGCCCAAA GTGCCTCTGA TAACAGCGGA GCAGAGGAGA CCCAAGGAAG TGTGGACGAG 
GTTTCACTGA AAGAATCGTC ACCGAGTGAT GACTCTATGA TTTCGCCACT TAGTGAAGAC 
TCTCAGGCTG AAGCAGAAGG TGTGTTTGTG TCTCCAAACA AACCTCGTAC AACTGAAGAT 
TTGTTTGCAG TCATTCACAG GTCGAAGAGG AAAGTACTTG GAAGAAAAGA TTCTGGGGAT 
ATGTCTGCTC GAAGCAAATC AAGAGCTTCT CTTGGCAGCA GTAGCAGCAG CAACGCTGGC 
TCTGTCACTT TGCCCAACAG CAGCGTGACC AGCCCAAACA GCCAGAGGTC TCCTGGCCTC 
ATCTACCGAA ATGCCAAAAA GTCCAACACA TCGAATGAAG AGTTTAAGCT GTTACTCCTC 
AAGAAAGGAA GTCGCTCAGA TTCTAGTTAC CGCATGTCTG CTACTGAGAT CCTGAAAAGT 
CCCATCCTGC CCAAACCTCC TGGAGAGCTC ACAGCTGAAT CCCCACAAAG CACCCAGGAG 
GCCCATCAGG GGGCACCTGG GGCCGAGGCT TTGTCCCCAC TCTCTCCATG CTCCCCAAGA 
GTTAATGCAG AAGGGTTTTC CTCAAAGAAC TTTGCCACCT CAGCAGCAGC AAGGGTGGGA 
CGCTCCCGAG CTCCCCCTGC GGCCAGCAGC AGCCGCTACA GCGTCCGCTG CAGGCTGTAC 
AATGCACCCA TGCAGGCCAT CTCTGAGGGT GAGACAGAAA ATTCTGATGG AAGCCCACAT 
GATGACCGAT CCTCCCAGAG CTCAACATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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NHS cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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