nhs cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	nhs
Entrez Gene ID	100498628
Full Name	NHS actin remodeling regulator
Synonyms	scml1
General protein information	Preferred Names NHS actin remodeling regulator Names Nance-Horan syndrome protein NHS-like protein 1-like Nance-Horan syndrome (congenital cataracts and dental anomalies)
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002934358.4	XP_002934404.2	Nance-Horan syndrome protein isoform X1
XM_018091600.1	XP_017947089.1	Nance-Horan syndrome protein isoform X2
XM_018091600.2	XP_017947089.1	Nance-Horan syndrome protein isoform X2
XM_031896962.1	XP_031752822.1	Nance-Horan syndrome protein isoform X3
XM_002934358.5	XP_002934404.2	Nance-Horan syndrome protein isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NHS	XP_548877.2
Bos taurus (cattle)	NHS	NP_001192657.1
Mus musculus (house mouse)	Nhs	NP_001074521.1
	NHS	XP_001104229.2
Rattus norvegicus (Norway rat)	Nhs	NP_001178662.1
Danio rerio (zebrafish)	nhs	XP_005169435.1
Gallus gallus (chicken)	NHS	XP_416815.4
Danio rerio (zebrafish)	nhsa	XP_001919340.4
Xenopus tropicalis (tropical clawed frog)	LOC100498628	XP_002934404.2
Homo sapiens (human)	NHS	NP_938011.1
Pan troglodytes (chimpanzee)	NHS	XP_520958.3

Gene Ontology

The following nhs gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nhs cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa52397	XM_031896962.1 Latest version!	Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OXa42770	XM_018091600.1 Latest version!	Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OXa52395	XM_002934358.5 Latest version!	Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa52396	XM_018091600.2 Latest version!	Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OXa05595	XM_002934358.4 Latest version!	Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nhs ( XM_031896962.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031752822.1 Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa52397
Clone ID Related Accession (Same CDS sequence)	XM_031896962.1
Accession Version	XM_031896962.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Nance-Horan syndrome protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGG AGTCCCAAGT GATTACTTCT TGCATTATAC CCATCAATGT TACTGGGGTT 
GGATTTGATA GAGAGGCGAG CATACGCTGT TCACTTGTTC ATTCACAATC TGTACTGCAG 
CGTAGACGGA AACTGAGAAG AAGGAAAACT ATATCAGGAA TTCCCAGAAG AGTTCAACAA 
GAAATAGATT CTGATGAATC CCCAGTTGCA AGAGAGCGTA ATGTTATAGT TCACACCAAC 
CCTGAACTTG CCAGCTCTGC AAACAGGAGA TCTGGTACAC GTGATACAGA GTGCCAAACA 
GATGATATAC TCATTACTGC TCCATCTCGT AGAAGAATTA GAGCTCAAAG GGGCCAGGGA 
GTTGCAGCAT CTCTCTCTCA TTCTGCTGGT AACATTGCAG CCCTAACAGA GAGTGGTGAC 
CAGATGTTTA AAACCACAGT ATCCAGTCGC ATTCGATCAA GGAGTCTTCC TAGAGAAGGT 
GTTAGAGCAA GCAATGATCA TGATTGTTGC ACACAGTCAT CAACATATGA AGAAGACTGC 
TATATGCCCT GCACTGAGAG GACACTAAAG AAGGATAAAG ACAGATTAAC TAGTCAGGAG 
TCTCCAGACC ACCAGCCATT AGGCTTATCC TATTCCCAAC ACCGACACAG CCCGCCGTTA 
AATTTGAATG AAAGGGGGAG ATCAAGGCTA TCAAGAATGG CAGATTCAGG GAGCTGTGAT 
ATCTCTTCAA ATTCAGATAC ATTTGGAAGC CCTGTTCACT CAATTTCAGC AGCAGGTGTG 
TTGTTGAGCA CACACATTGA TCAAAAAGAT GACCATCAGT CGTCTAGTGG AAACTGGAGC 
GGAAGTAGCT CAACCTGTCC CTCCCAGACA TCAGAAACAA TCCCTCCAGC TGCATCTCCT 
CCACTAACTG GTTCTTCCCA CTGTGATTCT GAATTATCCT TGAACACAGC TCCCCACGCT 
GGTGAAGATT CTAATGTTTT TATGATTGAA CAATACAGTG ATGACCACAT AGATAGCATA 
AGAGGTCACA GAGCCAGTTC CTTTACATCT ACAGTTGCTG ACCTGCTTGA CGATCCAAAC 
AACAGTAATA CTAGTGACAG CGAATGGAAT TACCTTCACC ATCACCATGA TGCATCATGT 
CATCAAGATT TTAGTCCACC GCACTTAAAG ACAGATCGCC TAGCATGCCC TAGTTTTACC 
AGTATGGGCA CTTATGACAG TTTTCTTGAA AAACCTGCAT CTGAAAAAGC AGATTCAGTT 
TCATATTATT CTGTAGACAC TGAAGGATAT TATACCTCCA TGCATTTTGA CTGTGGTCTG 
AATACTAGTA GGAGTTATAT TTGTAACTAT GCACCAACAG GGTCAGAAAG TGATCAAGGT 
CCTGGTGTTT CTTCTGTGAA TACCGATAAT CCTTGGCAGT GTTATATGGA TCAAGGCAAT 
GAAGGAAACC AGAACATCTC ATTTAAGAAA CCAAAGGCAA AGCCTACACC ACCAAAACGT 
TGTTCATCTT TAAGGAAATC TGACAACAAC ACAGATATTC CTGAGAAGAA AGAACCAAAG 
ATAGTATGTG GTCAGCAAGT GAATCACTCT TCCAGGGAAA TGAAGCTGCC ACTTGAGATT 
TCTAATGTTC CTTTAAGAGT GGAAAACTTT GCTACACAAC ATCAACAGGA AATACCATGG 
GCTAACCAGG ATGACACTGA GTTAAAGAAC ACTCAATTTT TAACCACAGA TATTACTTCT 
TTTAAAGATG AGAGTGGAGA GCAGTCTCAC TATGCAGATC TGTGGCTCCT CAATGACTTG 
AAAACAAGTG ATCCTTATAG GTCATTATCA AACTCAAGCA CTGCCACAGG TACTACAGTT 
ATAGAATGCA TTAAGTCACC AGAAAGTTCA GAGTCACAGA CATCACAGTC TGAATCCAGA 
GCAACGTCGC CATCTCTTCC TTCTGTGGAA AATGAGTTCA AGTTGTCTTC TCCAGAAAAG 
CTTGCTGGCT TAGCATCACC ATCAAGTGGT TACTCTAGCC AGTCAGAAAC CCCTACATCC 
TCCTTTCCCA TACCTTTCTT TTCTGGGCCC CTGTCACCTG GTGGTAGCAA ACGAAAGCCA 
AAAGTTCCAG AAAGAAAATC GTCCTTGCAA CATCCTTCTG CAAAATGTGG AAGAGAACTG 
GAGCTTCCAA CTATACCTCC GACCCATCTT GACCTAAGTG CTCTTCATGT TTTACATAAA 
CCATTACCTT ACAGACCACA GATGCATGCT TTCAGTCATA ATAATCAGAA TATGTCAGAA 
GATCAGATTG ACTCAAATTC TTCACCAGCT CTTGCCATAA CACCATCTGT ACTTAAATCT 
GTCCATCTCA GATCTGTCAA CAAGCAACAT AAAAAGTCAA AGCAAAGAGA AACCTGTAAT 
CTTGTGTGCT TGCAGGATCC TACAATAACA GTGGATACCT ATACCCCAGA AAAGGCAATT 
GTGACATCAG CCAGAAAATC AATTTTGCGT CAGTTTTCTA CAGAGGAAAC CATCCTACCA 
TATAAGGATT CCTCACCAGT TGACACCAGT CCAGAAAGTA TGTTTTTTGA CAAGTGTGCA 
CTATTCACTG GTCATGGAAT ATACAGAGAA GAGAAAGGTA CAAATATATT TGGAGTCACT 
AAAGAACCTT GGACTAATGA AATTGTGCAA GAAACTGTGT TTTTAGTACA GCCAGAGACC 
ACAACAACAA CTTTTCAAGA GTACTACGAG AAAAAGAACT GTGGCCATGC TCAAGATGAT 
CAACTTAAGA TGACACCAGA CTTACATATA CAGGATGATG TAATGACTAT ACCAGAGCCT 
GTAACAGGAA CAACTACTGG GTACACATTG CAAGACAATG CTGCTGGGAC ATCTTTAATG 
CCTAACTATG AGCCAGACTT GAGCAACAAT GATAAAGTGC CTGGAAACAC TCTCAGCTTT 
GAATCAGAGC TCCCCACTGT ATATTCACTC AGCGATGAAT GTCCAGAGCA GGAATCTGAA 
TCTGTATCAG GAATTCCAAC CAAAAGTGCC TCAGCGGACA AAATTAATGA TTCATCTGAC 
AACATGGAAG ACACCTTCTG CAAAGAATCA TCTCAGAGTG AGGACTACCT AATTTCACCA 
ATGAGTGAGG ACTCGGCAGA CACAGATGAT GTGTTTGTGT CCCCTAACAA ACCTCGTACA 
ACAGAGGATC TTTTTGCAGC CATTCACAGA TCCAAACGAA AAGTACTTGG AAGAAAAGAA 
GCTGGGGACA ACTCTGTGAA AAACAAATCA AAACCAATAA CTGGGAGCAG TGGGGCCTCT 
GCTAGCTCTC AAAAGTCTCC TGGTCTCATC TATAGGAGTG CTAAAAAATC CAATACATCC 
AATGAAGAAT TTAAGTTGCT GCTTTTGAAA AAGGGCAGCA GGTCAGATAC CAGCTACCGC 
ATGTCTGCTA CTGAGATTCT GAAGAGTCCA GTAACACCTA AATCCCTTGG TGATCTAGCA 
CTTGACTCCT CATCAAGCTC TGAAGAGGGT GGTCAGTTAT CACCTGGAAG TGATGCATTT 
TCGCCTTTAT CCCCTTGTTC TCCTCGTGTT ATATCTGAGG GGTTTTCTCC AAAAAGTGGA 
ACTATGTCTG CTGCCTCAAG AGTGGGAAGG TCCAGAGTGC CTCCTGCTGC AAGCAGCAGC 
CGTTACAGTG TCAGGTGCCG TTTTTACAAT GCTCCAATGC AGGCCATATC AGAAGGAGAA 
ACAGAAAACT CGGATGGTAG CCCTCATGAT GACAGGTCCT CTCAGAGCTC AATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031752822.1
CDS	2845..6621
Translation	MKQESQVITSCIIPINVTGVGFDREASIRCSLVHSQSVLQRRRKLRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPELASSANRRSGTRDTECQTDDILITAPSRRRIRAQRGQGVAASLSHSAGNIAALTESGDQMFKTTVSSRIRSRSLPREGVRASNDHDCCTQSSTYEEDCYMPCTERTLKKDKDRLTSQESPDHQPLGLSYSQHRHSPPLNLNERGRSRLSRMADSGSCDISSNSDTFGSPVHSISAAGVLLSTHIDQKDDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNTAPHAGEDSNVFMIEQYSDDHIDSIRGHRASSFTSTVADLLDDPNNSNTSDSEWNYLHHHHDASCHQDFSPPHLKTDRLACPSFTSMGTYDSFLEKPASEKADSVSYYSVDTEGYYTSMHFDCGLNTSRSYICNYAPTGSESDQGPGVSSVNTDNPWQCYMDQGNEGNQNISFKKPKAKPTPPKRCSSLRKSDNNTDIPEKKEPKIVCGQQVNHSSREMKLPLEISNVPLRVENFATQHQQEIPWANQDDTELKNTQFLTTDITSFKDESGEQSHYADLWLLNDLKTSDPYRSLSNSSTATGTTVIECIKSPESSESQTSQSESRATSPSLPSVENEFKLSSPEKLAGLASPSSGYSSQSETPTSSFPIPFFSGPLSPGGSKRKPKVPERKSSLQHPSAKCGRELELPTIPPTHLDLSALHVLHKPLPYRPQMHAFSHNNQNMSEDQIDSNSSPALAITPSVLKSVHLRSVNKQHKKSKQRETCNLVCLQDPTITVDTYTPEKAIVTSARKSILRQFSTEETILPYKDSSPVDTSPESMFFDKCALFTGHGIYREEKGTNIFGVTKEPWTNEIVQETVFLVQPETTTTTFQEYYEKKNCGHAQDDQLKMTPDLHIQDDVMTIPEPVTGTTTGYTLQDNAAGTSLMPNYEPDLSNNDKVPGNTLSFESELPTVYSLSDECPEQESESVSGIPTKSASADKINDSSDNMEDTFCKESSQSEDYLISPMSEDSADTDDVFVSPNKPRTTEDLFAAIHRSKRKVLGRKEAGDNSVKNKSKPITGSSGASASSQKSPGLIYRSAKKSNTSNEEFKLLLLKKGSRSDTSYRMSATEILKSPVTPKSLGDLALDSSSSSEEGGQLSPGSDAFSPLSPCSPRVISEGFSPKSGTMSAASRVGRSRVPPAASSSRYSVRCRFYNAPMQAISEGETENSDGSPHDDRSSQSSM

Target ORF information:

RefSeq Version	XM_031896962.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031896962.1

ORF Insert Sequence:

ATGAAACAGG AGTCCCAAGT GATTACTTCT TGCATTATAC CCATCAATGT TACTGGGGTT 
GGATTTGATA GAGAGGCGAG CATACGCTGT TCACTTGTTC ATTCACAATC TGTACTGCAG 
CGTAGACGGA AACTGAGAAG AAGGAAAACT ATATCAGGAA TTCCCAGAAG AGTTCAACAA 
GAAATAGATT CTGATGAATC CCCAGTTGCA AGAGAGCGTA ATGTTATAGT TCACACCAAC 
CCTGAACTTG CCAGCTCTGC AAACAGGAGA TCTGGTACAC GTGATACAGA GTGCCAAACA 
GATGATATAC TCATTACTGC TCCATCTCGT AGAAGAATTA GAGCTCAAAG GGGCCAGGGA 
GTTGCAGCAT CTCTCTCTCA TTCTGCTGGT AACATTGCAG CCCTAACAGA GAGTGGTGAC 
CAGATGTTTA AAACCACAGT ATCCAGTCGC ATTCGATCAA GGAGTCTTCC TAGAGAAGGT 
GTTAGAGCAA GCAATGATCA TGATTGTTGC ACACAGTCAT CAACATATGA AGAAGACTGC 
TATATGCCCT GCACTGAGAG GACACTAAAG AAGGATAAAG ACAGATTAAC TAGTCAGGAG 
TCTCCAGACC ACCAGCCATT AGGCTTATCC TATTCCCAAC ACCGACACAG CCCGCCGTTA 
AATTTGAATG AAAGGGGGAG ATCAAGGCTA TCAAGAATGG CAGATTCAGG GAGCTGTGAT 
ATCTCTTCAA ATTCAGATAC ATTTGGAAGC CCTGTTCACT CAATTTCAGC AGCAGGTGTG 
TTGTTGAGCA CACACATTGA TCAAAAAGAT GACCATCAGT CGTCTAGTGG AAACTGGAGC 
GGAAGTAGCT CAACCTGTCC CTCCCAGACA TCAGAAACAA TCCCTCCAGC TGCATCTCCT 
CCACTAACTG GTTCTTCCCA CTGTGATTCT GAATTATCCT TGAACACAGC TCCCCACGCT 
GGTGAAGATT CTAATGTTTT TATGATTGAA CAATACAGTG ATGACCACAT AGATAGCATA 
AGAGGTCACA GAGCCAGTTC CTTTACATCT ACAGTTGCTG ACCTGCTTGA CGATCCAAAC 
AACAGTAATA CTAGTGACAG CGAATGGAAT TACCTTCACC ATCACCATGA TGCATCATGT 
CATCAAGATT TTAGTCCACC GCACTTAAAG ACAGATCGCC TAGCATGCCC TAGTTTTACC 
AGTATGGGCA CTTATGACAG TTTTCTTGAA AAACCTGCAT CTGAAAAAGC AGATTCAGTT 
TCATATTATT CTGTAGACAC TGAAGGATAT TATACCTCCA TGCATTTTGA CTGTGGTCTG 
AATACTAGTA GGAGTTATAT TTGTAACTAT GCACCAACAG GGTCAGAAAG TGATCAAGGT 
CCTGGTGTTT CTTCTGTGAA TACCGATAAT CCTTGGCAGT GTTATATGGA TCAAGGCAAT 
GAAGGAAACC AGAACATCTC ATTTAAGAAA CCAAAGGCAA AGCCTACACC ACCAAAACGT 
TGTTCATCTT TAAGGAAATC TGACAACAAC ACAGATATTC CTGAGAAGAA AGAACCAAAG 
ATAGTATGTG GTCAGCAAGT GAATCACTCT TCCAGGGAAA TGAAGCTGCC ACTTGAGATT 
TCTAATGTTC CTTTAAGAGT GGAAAACTTT GCTACACAAC ATCAACAGGA AATACCATGG 
GCTAACCAGG ATGACACTGA GTTAAAGAAC ACTCAATTTT TAACCACAGA TATTACTTCT 
TTTAAAGATG AGAGTGGAGA GCAGTCTCAC TATGCAGATC TGTGGCTCCT CAATGACTTG 
AAAACAAGTG ATCCTTATAG GTCATTATCA AACTCAAGCA CTGCCACAGG TACTACAGTT 
ATAGAATGCA TTAAGTCACC AGAAAGTTCA GAGTCACAGA CATCACAGTC TGAATCCAGA 
GCAACGTCGC CATCTCTTCC TTCTGTGGAA AATGAGTTCA AGTTGTCTTC TCCAGAAAAG 
CTTGCTGGCT TAGCATCACC ATCAAGTGGT TACTCTAGCC AGTCAGAAAC CCCTACATCC 
TCCTTTCCCA TACCTTTCTT TTCTGGGCCC CTGTCACCTG GTGGTAGCAA ACGAAAGCCA 
AAAGTTCCAG AAAGAAAATC GTCCTTGCAA CATCCTTCTG CAAAATGTGG AAGAGAACTG 
GAGCTTCCAA CTATACCTCC GACCCATCTT GACCTAAGTG CTCTTCATGT TTTACATAAA 
CCATTACCTT ACAGACCACA GATGCATGCT TTCAGTCATA ATAATCAGAA TATGTCAGAA 
GATCAGATTG ACTCAAATTC TTCACCAGCT CTTGCCATAA CACCATCTGT ACTTAAATCT 
GTCCATCTCA GATCTGTCAA CAAGCAACAT AAAAAGTCAA AGCAAAGAGA AACCTGTAAT 
CTTGTGTGCT TGCAGGATCC TACAATAACA GTGGATACCT ATACCCCAGA AAAGGCAATT 
GTGACATCAG CCAGAAAATC AATTTTGCGT CAGTTTTCTA CAGAGGAAAC CATCCTACCA 
TATAAGGATT CCTCACCAGT TGACACCAGT CCAGAAAGTA TGTTTTTTGA CAAGTGTGCA 
CTATTCACTG GTCATGGAAT ATACAGAGAA GAGAAAGGTA CAAATATATT TGGAGTCACT 
AAAGAACCTT GGACTAATGA AATTGTGCAA GAAACTGTGT TTTTAGTACA GCCAGAGACC 
ACAACAACAA CTTTTCAAGA GTACTACGAG AAAAAGAACT GTGGCCATGC TCAAGATGAT 
CAACTTAAGA TGACACCAGA CTTACATATA CAGGATGATG TAATGACTAT ACCAGAGCCT 
GTAACAGGAA CAACTACTGG GTACACATTG CAAGACAATG CTGCTGGGAC ATCTTTAATG 
CCTAACTATG AGCCAGACTT GAGCAACAAT GATAAAGTGC CTGGAAACAC TCTCAGCTTT 
GAATCAGAGC TCCCCACTGT ATATTCACTC AGCGATGAAT GTCCAGAGCA GGAATCTGAA 
TCTGTATCAG GAATTCCAAC CAAAAGTGCC TCAGCGGACA AAATTAATGA TTCATCTGAC 
AACATGGAAG ACACCTTCTG CAAAGAATCA TCTCAGAGTG AGGACTACCT AATTTCACCA 
ATGAGTGAGG ACTCGGCAGA CACAGATGAT GTGTTTGTGT CCCCTAACAA ACCTCGTACA 
ACAGAGGATC TTTTTGCAGC CATTCACAGA TCCAAACGAA AAGTACTTGG AAGAAAAGAA 
GCTGGGGACA ACTCTGTGAA AAACAAATCA AAACCAATAA CTGGGAGCAG TGGGGCCTCT 
GCTAGCTCTC AAAAGTCTCC TGGTCTCATC TATAGGAGTG CTAAAAAATC CAATACATCC 
AATGAAGAAT TTAAGTTGCT GCTTTTGAAA AAGGGCAGCA GGTCAGATAC CAGCTACCGC 
ATGTCTGCTA CTGAGATTCT GAAGAGTCCA GTAACACCTA AATCCCTTGG TGATCTAGCA 
CTTGACTCCT CATCAAGCTC TGAAGAGGGT GGTCAGTTAT CACCTGGAAG TGATGCATTT 
TCGCCTTTAT CCCCTTGTTC TCCTCGTGTT ATATCTGAGG GGTTTTCTCC AAAAAGTGGA 
ACTATGTCTG CTGCCTCAAG AGTGGGAAGG TCCAGAGTGC CTCCTGCTGC AAGCAGCAGC 
CGTTACAGTG TCAGGTGCCG TTTTTACAAT GCTCCAATGC AGGCCATATC AGAAGGAGAA 
ACAGAAAACT CGGATGGTAG CCCTCATGAT GACAGGTCCT CTCAGAGCTC AATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhs ( XM_018091600.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947089.1 Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa42770
Clone ID Related Accession (Same CDS sequence)	XM_018091600.1
Accession Version	XM_018091600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Nance-Horan syndrome protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTTG CCAAGAGGAT TGTTGAGCCC CAGTGGCTGT GCAGGCATGC CATCACCCTT 
CAAGAGGGTA GCTGCTGCCG GGATCAGGGA GAAGAGCACA GGCAGCAGCA CCCCCGGGGC 
AACTCCAGGG AAGAATTGTC AGGGGTGGTG GCACCTTCAG TGCAGGTGCC CTCTGATGAA 
AGGGAAACAG TGCCCGGTGA TGGAAAAGAT AAAGATGTGG CGCCACTGGA TTTGGGCGCA 
TTCAACAACG TCGCCTTGTC ACGGATTTTG CGTCAGTTGT CGGACATGGC CCGGCATGCC 
TGCTCCCTTT TCCATGAGAT TGAGGTGGAT ATACAGGTGA TCGGTCGGCG GGTGAGAACA 
TTGCAAGGGA AAATCAGCAA TGTCCAGCAG GTTGTGACCA GTCTGGATCC AAAACAGGAG 
ACAGTCCCGG TCTCAAACCT GGATGAAGAA AGCAAACTGG AAGTGTACTA CAAGGCCAAC 
TGGCACCAGC AGAGGAATAT TTTCCTGCCA AACACGAGGC CCCCGTGTGT GGAAGAGTTG 
CACCGCCATG CCAAGCAGAG TCTGCGTTCA ATACGGAGAG AGCAACGGAT CCGGGCAGAT 
AATAGAGAAA GAAGGTTTCA GGGCTCGATA TTTACAGCCC CACCGCTTCC CACATACCCA 
GCAGTTCACA GTCTGAAGAG ACGAGAAGTC AAAGAGCACC ATATTGTACA AGCTCATCCA 
CCTCAGGAGG AAGATGATAT CACTTTAGGA CAAAGGCCGA AAAACCCTAT TCCCAATGTT 
CCATCTACAC TGGATAAGCA AACCAACTGG AACAAGGCTT TACCTCTCCC CACTCCTGAG 
GAGAAGATGA AACAGGAGTC CCAAGTGATT ACTTCTTGCA TTATACCCAT CAATGTTACT 
GGGGTTGGAT TTGATAGAGA GGCGAGCATA CGCTGTTCAC TTGTTCATTC ACAATCTGTA 
CTGCAGCGTA GACGGAAACT GAGAAGAAGG AAAACTATAT CAGGAATTCC CAGAAGAGTT 
CAACAAGAAA TAGATTCTGA TGAATCCCCA GTTGCAAGAG AGCGTAATGT TATAGTTCAC 
ACCAACCCTG AACTTGCCAG CTCTGCAAAC AGGAGATCTG GTACACGTGA TACAGAGTGC 
CAAACAGATG ATATACTCAT TACTGCTCCA TCTCGTAGAA GAATTAGAGC TCAAAGGGGC 
CAGGGAGTTG CAGCATCTCT CTCTCATTCT GCTGGTAACA TTGCAGCCCT AACAGAGAGT 
GGTGACCAGA TGTTTAAAAC CACAGTATCC AGTCGCATTC GATCAAGGAG TCTTCCTAGA 
GAAGGTGTTA GAGCAAGCAA TGATCATGAT TGTTGCACAC AGTCATCAAC ATATGAAGAA 
GACTGCTATA TGCCCTGCAC TGAGAGGACA CTAAAGAAGG ATAAAGACAG ATTAACTAGT 
CAGGAGTCTC CAGACCACCA GCCATTAGGC TTATCCTATT CCCAACACCG ACACAGCCCG 
CCGTTAAATT TGAATGAAAG GGGGAGATCA AGGCTATCAA GAATGGCAGA TTCAGGGAGC 
TGTGATATCT CTTCAAATTC AGATACATTT GGAAGCCCTG TTCACTCAAT TTCAGCAGCA 
GGTGTGTTGT TGAGCACACA CATTGATCAA AAAGATGACC ATCAGTCGTC TAGTGGAAAC 
TGGAGCGGAA GTAGCTCAAC CTGTCCCTCC CAGACATCAG AAACAATCCC TCCAGCTGCA 
TCTCCTCCAC TAACTGGTTC TTCCCACTGT GATTCTGAAT TATCCTTGAA CACAGCTCCC 
CACGCTGGTG AAGATTCTAA TGTTTTTATG ATTGAACAAT ACAGTGATGA CCACATAGAT 
AGCATAAGAG GTCACAGAGC CAGTTCCTTT ACATCTACAG TTGCTGACCT GCTTGACGAT 
CCAAACAACA GTAATACTAG TGACAGCGAA TGGAATTACC TTCACCATCA CCATGATGCA 
TCATGTCATC AAGATTTTAG TCCACCGCAC TTAAAGACAG ATCGCCTAGC ATGCCCTAGT 
TTTACCAGTA TGGGCACTTA TGACAGTTTT CTTGAAAAAC CTGCATCTGA AAAAGCAGAT 
TCAGTTTCAT ATTATTCTGT AGACACTGAA GGATATTATA CCTCCATGCA TTTTGACTGT 
GGTCTGAATA CTAGTAGGAG TTATATTTGT AACTATGCAC CAACAGGGTC AGAAAGTGAT 
CAAGGTCCTG GTGTTTCTTC TGTGAATACC GATAATCCTT GGCAGTGTTA TATGGATCAA 
GGCAATGAAG GAAACCAGAA CATCTCATTT AAGAAACCAA AGGCAAAGCC TACACCACCA 
AAACGTTGTT CATCTTTAAG GAAATCTGAC AACAACACAG ATATTCCTGA GAAGAAAGAA 
CCAAAGATAG TATGTGGTCA GCAAGTGAAT CACTCTTCCA GGGAAATGAA GCTGCCACTT 
GAGATTTCTA ATGTTCCTTT AAGAGTGGAA AACTTTGCTA CACAACATCA ACAGGAAATA 
CCATGGGCTA ACCAGGATGA CACTGAGTTA AAGAACACTC AATTTTTAAC CACAGATATT 
ACTTCTTTTA AAGATGAGAG TGGAGAGCAG TCTCACTATG CAGATCTGTG GCTCCTCAAT 
GACTTGAAAA CAAGTGATCC TTATAGGTCA TTATCAAACT CAAGCACTGC CACAGGTACT 
ACAGTTATAG AATGCATTAA GTCACCAGAA AGTTCAGAGT CACAGACATC ACAGTCTGAA 
TCCAGAGCAA CGTCGCCATC TCTTCCTTCT GTGGAAAATG AGTTCAAGTT GTCTTCTCCA 
GAAAAGCTTG CTGGCTTAGC ATCACCATCA AGTGGTTACT CTAGCCAGTC AGAAACCCCT 
ACATCCTCCT TTCCCATACC TTTCTTTTCT GGGCCCCTGT CACCTGGTGG TAGCAAACGA 
AAGCCAAAAG TTCCAGAAAG AAAATCGTCC TTGCAACATC CTTCTGCAAA ATGTGGAAGA 
GAACTGGAGC TTCCAACTAT ACCTCCGACC CATCTTGACC TAAGTGCTCT TCATGTTTTA 
CATAAACCAT TACCTTACAG ACCACAGATG CATGCTTTCA GTCATAATAA TCAGAATATG 
TCAGAAGATC AGATTGACTC AAATTCTTCA CCAGCTCTTG CCATAACACC ATCTGTACTT 
AAATCTGTCC ATCTCAGATC TGTCAACAAG CAACATAAAA AGTCAAAGCA AAGAGAAACC 
TGTAATCTTG TGTGCTTGCA GGATCCTACA ATAACAGTGG ATACCTATAC CCCAGAAAAG 
GCAATTGTGA CATCAGCCAG AAAATCAATT TTGCGTCAGT TTTCTACAGA GGAAACCATC 
CTACCATATA AGGATTCCTC ACCAGTTGAC ACCAGTCCAG AAAGTATGTT TTTTGACAAG 
TGTGCACTAT TCACTGGTCA TGGAATATAC AGAGAAGAGA AAGGTACAAA TATATTTGGA 
GTCACTAAAG AACCTTGGAC TAATGAAATT GTGCAAGAAA CTGTGTTTTT AGTACAGCCA 
GAGACCACAA CAACAACTTT TCAAGAGTAC TACGAGAAAA AGAACTGTGG CCATGCTCAA 
GATGATCAAC TTAAGATGAC ACCAGACTTA CATATACAGG ATGATGTAAT GACTATACCA 
GAGCCTGTAA CAGGAACAAC TACTGGGTAC ACATTGCAAG ACAATGCTGC TGGGACATCT 
TTAATGCCTA ACTATGAGCC AGACTTGAGC AACAATGATA AAGTGCCTGG AAACACTCTC 
AGCTTTGAAT CAGAGCTCCC CACTGTATAT TCACTCAGCG ATGAATGTCC AGAGCAGGAA 
TCTGAATCTG TATCAGGAAT TCCAACCAAA AGTGCCTCAG CGGACAAAAT TAATGATTCA 
TCTGACAACA TGGAAGACAC CTTCTGCAAA GAATCATCTC AGAGTGAGGA CTACCTAATT 
TCACCAATGA GTGAGGACTC GGCAGACACA GATGATGTGT TTGTGTCCCC TAACAAACCT 
CGTACAACAG AGGATCTTTT TGCAGCCATT CACAGATCCA AACGAAAAGT ACTTGGAAGA 
AAAGAAGCTG GGGACAACTC TGTGAAAAAC AAATCAAAAC CAATAACTGG GAGCAGTGGG 
GCCTCTGCTA GCTCTCAAAA GTCTCCTGGT CTCATCTATA GGAGTGCTAA AAAATCCAAT 
ACATCCAATG AAGAATTTAA GTTGCTGCTT TTGAAAAAGG GCAGCAGGTC AGATACCAGC 
TACCGCATGT CTGCTACTGA GATTCTGAAG AGTCCAGTAA CACCTAAATC CCTTGGTGAT 
CTAGCACTTG ACTCCTCATC AAGCTCTGAA GAGGGTGGTC AGTTATCACC TGGAAGTGAT 
GCATTTTCGC CTTTATCCCC TTGTTCTCCT CGTGTTATAT CTGAGGGGTT TTCTCCAAAA 
AGTGGAACTA TGTCTGCTGC CTCAAGAGTG GGAAGGTCCA GAGTGCCTCC TGCTGCAAGC 
AGCAGCCGTT ACAGTGTCAG GTGCCGTTTT TACAATGCTC CAATGCAGGC CATATCAGAA 
GGAGAAACAG AAAACTCGGA TGGTAGCCCT CATGATGACA GGTCCTCTCA GAGCTCAATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947089.1
CDS	1826..6448
Translation	MPFAKRIVEPQWLCRHAITLQEGSCCRDQGEEHRQQHPRGNSREELSGVVAPSVQVPSDERETVPGDGKDKDVAPLDLGAFNNVALSRILRQLSDMARHACSLFHEIEVDIQVIGRRVRTLQGKISNVQQVVTSLDPKQETVPVSNLDEESKLEVYYKANWHQQRNIFLPNTRPPCVEELHRHAKQSLRSIRREQRIRADNRERRFQGSIFTAPPLPTYPAVHSLKRREVKEHHIVQAHPPQEEDDITLGQRPKNPIPNVPSTLDKQTNWNKALPLPTPEEKMKQESQVITSCIIPINVTGVGFDREASIRCSLVHSQSVLQRRRKLRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPELASSANRRSGTRDTECQTDDILITAPSRRRIRAQRGQGVAASLSHSAGNIAALTESGDQMFKTTVSSRIRSRSLPREGVRASNDHDCCTQSSTYEEDCYMPCTERTLKKDKDRLTSQESPDHQPLGLSYSQHRHSPPLNLNERGRSRLSRMADSGSCDISSNSDTFGSPVHSISAAGVLLSTHIDQKDDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNTAPHAGEDSNVFMIEQYSDDHIDSIRGHRASSFTSTVADLLDDPNNSNTSDSEWNYLHHHHDASCHQDFSPPHLKTDRLACPSFTSMGTYDSFLEKPASEKADSVSYYSVDTEGYYTSMHFDCGLNTSRSYICNYAPTGSESDQGPGVSSVNTDNPWQCYMDQGNEGNQNISFKKPKAKPTPPKRCSSLRKSDNNTDIPEKKEPKIVCGQQVNHSSREMKLPLEISNVPLRVENFATQHQQEIPWANQDDTELKNTQFLTTDITSFKDESGEQSHYADLWLLNDLKTSDPYRSLSNSSTATGTTVIECIKSPESSESQTSQSESRATSPSLPSVENEFKLSSPEKLAGLASPSSGYSSQSETPTSSFPIPFFSGPLSPGGSKRKPKVPERKSSLQHPSAKCGRELELPTIPPTHLDLSALHVLHKPLPYRPQMHAFSHNNQNMSEDQIDSNSSPALAITPSVLKSVHLRSVNKQHKKSKQRETCNLVCLQDPTITVDTYTPEKAIVTSARKSILRQFSTEETILPYKDSSPVDTSPESMFFDKCALFTGHGIYREEKGTNIFGVTKEPWTNEIVQETVFLVQPETTTTTFQEYYEKKNCGHAQDDQLKMTPDLHIQDDVMTIPEPVTGTTTGYTLQDNAAGTSLMPNYEPDLSNNDKVPGNTLSFESELPTVYSLSDECPEQESESVSGIPTKSASADKINDSSDNMEDTFCKESSQSEDYLISPMSEDSADTDDVFVSPNKPRTTEDLFAAIHRSKRKVLGRKEAGDNSVKNKSKPITGSSGASASSQKSPGLIYRSAKKSNTSNEEFKLLLLKKGSRSDTSYRMSATEILKSPVTPKSLGDLALDSSSSSEEGGQLSPGSDAFSPLSPCSPRVISEGFSPKSGTMSAASRVGRSRVPPAASSSRYSVRCRFYNAPMQAISEGETENSDGSPHDDRSSQSSM

Target ORF information:

RefSeq Version	XM_018091600.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091600.1

ORF Insert Sequence:

ATGCCCTTTG CCAAGAGGAT TGTTGAGCCC CAGTGGCTGT GCAGGCATGC CATCACCCTT 
CAAGAGGGTA GCTGCTGCCG GGATCAGGGA GAAGAGCACA GGCAGCAGCA CCCCCGGGGC 
AACTCCAGGG AAGAATTGTC AGGGGTGGTG GCACCTTCAG TGCAGGTGCC CTCTGATGAA 
AGGGAAACAG TGCCCGGTGA TGGAAAAGAT AAAGATGTGG CGCCACTGGA TTTGGGCGCA 
TTCAACAACG TCGCCTTGTC ACGGATTTTG CGTCAGTTGT CGGACATGGC CCGGCATGCC 
TGCTCCCTTT TCCATGAGAT TGAGGTGGAT ATACAGGTGA TCGGTCGGCG GGTGAGAACA 
TTGCAAGGGA AAATCAGCAA TGTCCAGCAG GTTGTGACCA GTCTGGATCC AAAACAGGAG 
ACAGTCCCGG TCTCAAACCT GGATGAAGAA AGCAAACTGG AAGTGTACTA CAAGGCCAAC 
TGGCACCAGC AGAGGAATAT TTTCCTGCCA AACACGAGGC CCCCGTGTGT GGAAGAGTTG 
CACCGCCATG CCAAGCAGAG TCTGCGTTCA ATACGGAGAG AGCAACGGAT CCGGGCAGAT 
AATAGAGAAA GAAGGTTTCA GGGCTCGATA TTTACAGCCC CACCGCTTCC CACATACCCA 
GCAGTTCACA GTCTGAAGAG ACGAGAAGTC AAAGAGCACC ATATTGTACA AGCTCATCCA 
CCTCAGGAGG AAGATGATAT CACTTTAGGA CAAAGGCCGA AAAACCCTAT TCCCAATGTT 
CCATCTACAC TGGATAAGCA AACCAACTGG AACAAGGCTT TACCTCTCCC CACTCCTGAG 
GAGAAGATGA AACAGGAGTC CCAAGTGATT ACTTCTTGCA TTATACCCAT CAATGTTACT 
GGGGTTGGAT TTGATAGAGA GGCGAGCATA CGCTGTTCAC TTGTTCATTC ACAATCTGTA 
CTGCAGCGTA GACGGAAACT GAGAAGAAGG AAAACTATAT CAGGAATTCC CAGAAGAGTT 
CAACAAGAAA TAGATTCTGA TGAATCCCCA GTTGCAAGAG AGCGTAATGT TATAGTTCAC 
ACCAACCCTG AACTTGCCAG CTCTGCAAAC AGGAGATCTG GTACACGTGA TACAGAGTGC 
CAAACAGATG ATATACTCAT TACTGCTCCA TCTCGTAGAA GAATTAGAGC TCAAAGGGGC 
CAGGGAGTTG CAGCATCTCT CTCTCATTCT GCTGGTAACA TTGCAGCCCT AACAGAGAGT 
GGTGACCAGA TGTTTAAAAC CACAGTATCC AGTCGCATTC GATCAAGGAG TCTTCCTAGA 
GAAGGTGTTA GAGCAAGCAA TGATCATGAT TGTTGCACAC AGTCATCAAC ATATGAAGAA 
GACTGCTATA TGCCCTGCAC TGAGAGGACA CTAAAGAAGG ATAAAGACAG ATTAACTAGT 
CAGGAGTCTC CAGACCACCA GCCATTAGGC TTATCCTATT CCCAACACCG ACACAGCCCG 
CCGTTAAATT TGAATGAAAG GGGGAGATCA AGGCTATCAA GAATGGCAGA TTCAGGGAGC 
TGTGATATCT CTTCAAATTC AGATACATTT GGAAGCCCTG TTCACTCAAT TTCAGCAGCA 
GGTGTGTTGT TGAGCACACA CATTGATCAA AAAGATGACC ATCAGTCGTC TAGTGGAAAC 
TGGAGCGGAA GTAGCTCAAC CTGTCCCTCC CAGACATCAG AAACAATCCC TCCAGCTGCA 
TCTCCTCCAC TAACTGGTTC TTCCCACTGT GATTCTGAAT TATCCTTGAA CACAGCTCCC 
CACGCTGGTG AAGATTCTAA TGTTTTTATG ATTGAACAAT ACAGTGATGA CCACATAGAT 
AGCATAAGAG GTCACAGAGC CAGTTCCTTT ACATCTACAG TTGCTGACCT GCTTGACGAT 
CCAAACAACA GTAATACTAG TGACAGCGAA TGGAATTACC TTCACCATCA CCATGATGCA 
TCATGTCATC AAGATTTTAG TCCACCGCAC TTAAAGACAG ATCGCCTAGC ATGCCCTAGT 
TTTACCAGTA TGGGCACTTA TGACAGTTTT CTTGAAAAAC CTGCATCTGA AAAAGCAGAT 
TCAGTTTCAT ATTATTCTGT AGACACTGAA GGATATTATA CCTCCATGCA TTTTGACTGT 
GGTCTGAATA CTAGTAGGAG TTATATTTGT AACTATGCAC CAACAGGGTC AGAAAGTGAT 
CAAGGTCCTG GTGTTTCTTC TGTGAATACC GATAATCCTT GGCAGTGTTA TATGGATCAA 
GGCAATGAAG GAAACCAGAA CATCTCATTT AAGAAACCAA AGGCAAAGCC TACACCACCA 
AAACGTTGTT CATCTTTAAG GAAATCTGAC AACAACACAG ATATTCCTGA GAAGAAAGAA 
CCAAAGATAG TATGTGGTCA GCAAGTGAAT CACTCTTCCA GGGAAATGAA GCTGCCACTT 
GAGATTTCTA ATGTTCCTTT AAGAGTGGAA AACTTTGCTA CACAACATCA ACAGGAAATA 
CCATGGGCTA ACCAGGATGA CACTGAGTTA AAGAACACTC AATTTTTAAC CACAGATATT 
ACTTCTTTTA AAGATGAGAG TGGAGAGCAG TCTCACTATG CAGATCTGTG GCTCCTCAAT 
GACTTGAAAA CAAGTGATCC TTATAGGTCA TTATCAAACT CAAGCACTGC CACAGGTACT 
ACAGTTATAG AATGCATTAA GTCACCAGAA AGTTCAGAGT CACAGACATC ACAGTCTGAA 
TCCAGAGCAA CGTCGCCATC TCTTCCTTCT GTGGAAAATG AGTTCAAGTT GTCTTCTCCA 
GAAAAGCTTG CTGGCTTAGC ATCACCATCA AGTGGTTACT CTAGCCAGTC AGAAACCCCT 
ACATCCTCCT TTCCCATACC TTTCTTTTCT GGGCCCCTGT CACCTGGTGG TAGCAAACGA 
AAGCCAAAAG TTCCAGAAAG AAAATCGTCC TTGCAACATC CTTCTGCAAA ATGTGGAAGA 
GAACTGGAGC TTCCAACTAT ACCTCCGACC CATCTTGACC TAAGTGCTCT TCATGTTTTA 
CATAAACCAT TACCTTACAG ACCACAGATG CATGCTTTCA GTCATAATAA TCAGAATATG 
TCAGAAGATC AGATTGACTC AAATTCTTCA CCAGCTCTTG CCATAACACC ATCTGTACTT 
AAATCTGTCC ATCTCAGATC TGTCAACAAG CAACATAAAA AGTCAAAGCA AAGAGAAACC 
TGTAATCTTG TGTGCTTGCA GGATCCTACA ATAACAGTGG ATACCTATAC CCCAGAAAAG 
GCAATTGTGA CATCAGCCAG AAAATCAATT TTGCGTCAGT TTTCTACAGA GGAAACCATC 
CTACCATATA AGGATTCCTC ACCAGTTGAC ACCAGTCCAG AAAGTATGTT TTTTGACAAG 
TGTGCACTAT TCACTGGTCA TGGAATATAC AGAGAAGAGA AAGGTACAAA TATATTTGGA 
GTCACTAAAG AACCTTGGAC TAATGAAATT GTGCAAGAAA CTGTGTTTTT AGTACAGCCA 
GAGACCACAA CAACAACTTT TCAAGAGTAC TACGAGAAAA AGAACTGTGG CCATGCTCAA 
GATGATCAAC TTAAGATGAC ACCAGACTTA CATATACAGG ATGATGTAAT GACTATACCA 
GAGCCTGTAA CAGGAACAAC TACTGGGTAC ACATTGCAAG ACAATGCTGC TGGGACATCT 
TTAATGCCTA ACTATGAGCC AGACTTGAGC AACAATGATA AAGTGCCTGG AAACACTCTC 
AGCTTTGAAT CAGAGCTCCC CACTGTATAT TCACTCAGCG ATGAATGTCC AGAGCAGGAA 
TCTGAATCTG TATCAGGAAT TCCAACCAAA AGTGCCTCAG CGGACAAAAT TAATGATTCA 
TCTGACAACA TGGAAGACAC CTTCTGCAAA GAATCATCTC AGAGTGAGGA CTACCTAATT 
TCACCAATGA GTGAGGACTC GGCAGACACA GATGATGTGT TTGTGTCCCC TAACAAACCT 
CGTACAACAG AGGATCTTTT TGCAGCCATT CACAGATCCA AACGAAAAGT ACTTGGAAGA 
AAAGAAGCTG GGGACAACTC TGTGAAAAAC AAATCAAAAC CAATAACTGG GAGCAGTGGG 
GCCTCTGCTA GCTCTCAAAA GTCTCCTGGT CTCATCTATA GGAGTGCTAA AAAATCCAAT 
ACATCCAATG AAGAATTTAA GTTGCTGCTT TTGAAAAAGG GCAGCAGGTC AGATACCAGC 
TACCGCATGT CTGCTACTGA GATTCTGAAG AGTCCAGTAA CACCTAAATC CCTTGGTGAT 
CTAGCACTTG ACTCCTCATC AAGCTCTGAA GAGGGTGGTC AGTTATCACC TGGAAGTGAT 
GCATTTTCGC CTTTATCCCC TTGTTCTCCT CGTGTTATAT CTGAGGGGTT TTCTCCAAAA 
AGTGGAACTA TGTCTGCTGC CTCAAGAGTG GGAAGGTCCA GAGTGCCTCC TGCTGCAAGC 
AGCAGCCGTT ACAGTGTCAG GTGCCGTTTT TACAATGCTC CAATGCAGGC CATATCAGAA 
GGAGAAACAG AAAACTCGGA TGGTAGCCCT CATGATGACA GGTCCTCTCA GAGCTCAATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhs ( XM_002934358.5 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934404.2 Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X1, mRNA.

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CloneID	OXa52395
Clone ID Related Accession (Same CDS sequence)	XM_002934358.5
Accession Version	XM_002934358.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Nance-Horan syndrome protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002934358.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTTG CCAAGAGGAT TGTTGAGCCC CAGTGGCTGT GCAGGCATGC CATCACCCTT 
CAAGAGGGTA GCTGCTGCCG GGATCAGGGA GAAGAGCACA GGCAGCAGCA CCCCCGGGGC 
AACTCCAGGG AAGAATTGTC AGGGGTGGTG GCACCTTCAG TGCAGGTGCC CTCTGATGAA 
AGGGAAACAG TGCCCGGTGA TGGAAAAGAT AAAGATGTGG CGCCACTGGA TTTGGGCGCA 
TTCAACAACG TCGCCTTGTC ACGGATTTTG CGTCAGTTGT CGGACATGGC CCGGCATGCC 
TGCTCCCTTT TCCATGAGAT TGAGGTGGAT ATACAGGTGA TCGGTCGGCG GGTGAGAACA 
TTGCAAGGGA AAATCAGCAA TGTCCAGCAG GTTGTGACCA GTCTGGATCC AAAACAGGAG 
ACAGTCCCGG TCTCAAACCT GGATGAAGAA AGCAAACTGG AAGTGTACTA CAAGGCCAAC 
TGGCACCAGC AGAGGAATAT TTTCCTGCCA AACACGAGGC CCCCGTGTGT GGAAGAGTTG 
CACCGCCATG CCAAGCAGAG TCTGCGTTCA ATACGGAGAG AGCAACGGAT CCGGGCAGAT 
AATAGAGAAA GAAGGTTTCA GGGCTCGATA TTTACAGCCC CACCGCTTCC CACATACCCA 
GCAGTTCACA GTTTGAAGAG ACGAGAAGTC AAAGAGCACC ATATTGTACA ATTTAACAGA 
ACCCGTTCGC ACTCCCCCAC TGAATGTTGC CATTTGACCC CATGGAGTAG AAAGGCTCAT 
CCACCTCAGG AGGAAGATGA TATCACTTTA GGACAAAGGC CGAAAAACCC TATTCCCAAT 
GTTCCATCTA CACTGGATAA GCAAACCAAC TGGAACAAGG CTTTACCTCT CCCCACTCCT 
GAGGAGAAGA TGAAACAGGA GTCCCAAGTG ATTACTTCTT GCATTATACC CATCAATGTT 
ACTGGGGTTG GATTTGATAG AGAGGCGAGC ATACGCTGTT CACTTGTTCA TTCACAATCT 
GTACTGCAGC GTAGACGGAA ACTGAGAAGA AGGAAAACTA TATCAGGAAT TCCCAGAAGA 
GTTCAACAAG AAATAGATTC TGATGAATCC CCAGTTGCAA GAGAGCGTAA TGTTATAGTT 
CACACCAACC CTGAACTTGC CAGCTCTGCA AACAGGAGAT CTGGTACACG TGATACAGAG 
TGCCAAACAG ATGATATACT CATTACTGCT CCATCTCGTA GAAGAATTAG AGCTCAAAGG 
GGCCAGGGAG TTGCAGCATC TCTCTCTCAT TCTGCTGGTA ACATTGCAGC CCTAACAGAG 
AGTGGTGACC AGATGTTTAA AACCACAGTA TCCAGTCGCA TTCGATCAAG GAGTCTTCCT 
AGAGAAGGTG TTAGAGCAAG CAATGATCAT GATTGTTGCA CACAGTCATC AACATATGAA 
GAAGACTGCT ATATGCCCTG CACTGAGAGG ACACTAAAGA AGGATAAAGA CAGATTAACT 
AGTCAGGAGT CTCCAGACCA CCAGCCATTA GGCTTATCCT ATTCCCAACA CCGACACAGC 
CCGCCGTTAA ATTTGAATGA AAGGGGGAGA TCAAGGCTAT CAAGAATGGC AGATTCAGGG 
AGCTGTGATA TCTCTTCAAA TTCAGATACA TTTGGAAGCC CTGTTCACTC AATTTCAGCA 
GCAGGTGTGT TGTTGAGCAC ACACATTGAT CAAAAAGATG ACCATCAGTC GTCTAGTGGA 
AACTGGAGCG GAAGTAGCTC AACCTGTCCC TCCCAGACAT CAGAAACAAT CCCTCCAGCT 
GCATCTCCTC CACTAACTGG TTCTTCCCAC TGTGATTCTG AATTATCCTT GAACACAGCT 
CCCCACGCTG GTGAAGATTC TAATGTTTTT ATGATTGAAC AATACAGTGA TGACCACATA 
GATAGCATAA GAGGTCACAG AGCCAGTTCC TTTACATCTA CAGTTGCTGA CCTGCTTGAC 
GATCCAAACA ACAGTAATAC TAGTGACAGC GAATGGAATT ACCTTCACCA TCACCATGAT 
GCATCATGTC ATCAAGATTT TAGTCCACCG CACTTAAAGA CAGATCGCCT AGCATGCCCT 
AGTTTTACCA GTATGGGCAC TTATGACAGT TTTCTTGAAA AACCTGCATC TGAAAAAGCA 
GATTCAGTTT CATATTATTC TGTAGACACT GAAGGATATT ATACCTCCAT GCATTTTGAC 
TGTGGTCTGA ATACTAGTAG GAGTTATATT TGTAACTATG CACCAACAGG GTCAGAAAGT 
GATCAAGGTC CTGGTGTTTC TTCTGTGAAT ACCGATAATC CTTGGCAGTG TTATATGGAT 
CAAGGCAATG AAGGAAACCA GAACATCTCA TTTAAGAAAC CAAAGGCAAA GCCTACACCA 
CCAAAACGTT GTTCATCTTT AAGGAAATCT GACAACAACA CAGATATTCC TGAGAAGAAA 
GAACCAAAGA TAGTATGTGG TCAGCAAGTG AATCACTCTT CCAGGGAAAT GAAGCTGCCA 
CTTGAGATTT CTAATGTTCC TTTAAGAGTG GAAAACTTTG CTACACAACA TCAACAGGAA 
ATACCATGGG CTAACCAGGA TGACACTGAG TTAAAGAACA CTCAATTTTT AACCACAGAT 
ATTACTTCTT TTAAAGATGA GAGTGGAGAG CAGTCTCACT ATGCAGATCT GTGGCTCCTC 
AATGACTTGA AAACAAGTGA TCCTTATAGG TCATTATCAA ACTCAAGCAC TGCCACAGGT 
ACTACAGTTA TAGAATGCAT TAAGTCACCA GAAAGTTCAG AGTCACAGAC ATCACAGTCT 
GAATCCAGAG CAACGTCGCC ATCTCTTCCT TCTGTGGAAA ATGAGTTCAA GTTGTCTTCT 
CCAGAAAAGC TTGCTGGCTT AGCATCACCA TCAAGTGGTT ACTCTAGCCA GTCAGAAACC 
CCTACATCCT CCTTTCCCAT ACCTTTCTTT TCTGGGCCCC TGTCACCTGG TGGTAGCAAA 
CGAAAGCCAA AAGTTCCAGA AAGAAAATCG TCCTTGCAAC ATCCTTCTGC AAAATGTGGA 
AGAGAACTGG AGCTTCCAAC TATACCTCCG ACCCATCTTG ACCTAAGTGC TCTTCATGTT 
TTACATAAAC CATTACCTTA CAGACCACAG ATGCATGCTT TCAGTCATAA TAATCAGAAT 
ATGTCAGAAG ATCAGATTGA CTCAAATTCT TCACCAGCTC TTGCCATAAC ACCATCTGTA 
CTTAAATCTG TCCATCTCAG ATCTGTCAAC AAGCAACATA AAAAGTCAAA GCAAAGAGAA 
ACCTGTAATC TTGTGTGCTT GCAGGATCCT ACAATAACAG TGGATACCTA TACCCCAGAA 
AAGGCAATTG TGACATCAGC CAGAAAATCA ATTTTGCGTC AGTTTTCTAC AGAGGAAACC 
ATCCTACCAT ATAAGGATTC CTCACCAGTT GACACCAGTC CAGAAAGTAT GTTTTTTGAC 
AAGTGTGCAC TATTCACTGG TCATGGAATA TACAGAGAAG AGAAAGGTAC AAATATATTT 
GGAGTCACTA AAGAACCTTG GACTAATGAA ATTGTGCAAG AAACTGTGTT TTTAGTACAG 
CCAGAGACCA CAACAACAAC TTTTCAAGAG TACTACGAGA AAAAGAACTG TGGCCATGCT 
CAAGATGATC AACTTAAGAT GACACCAGAC TTACATATAC AGGATGATGT AATGACTATA 
CCAGAGCCTG TAACAGGAAC AACTACTGGG TACACATTGC AAGACAATGC TGCTGGGACA 
TCTTTAATGC CTAACTATGA GCCAGACTTG AGCAACAATG ATAAAGTGCC TGGAAACACT 
CTCAGCTTTG AATCAGAGCT CCCCACTGTA TATTCACTCA GCGATGAATG TCCAGAGCAG 
GAATCTGAAT CTGTATCAGG AATTCCAACC AAAAGTGCCT CAGCGGACAA AATTAATGAT 
TCATCTGACA ACATGGAAGA CACCTTCTGC AAAGAATCAT CTCAGAGTGA GGACTACCTA 
ATTTCACCAA TGAGTGAGGA CTCGGCAGAC ACAGATGATG TGTTTGTGTC CCCTAACAAA 
CCTCGTACAA CAGAGGATCT TTTTGCAGCC ATTCACAGAT CCAAACGAAA AGTACTTGGA 
AGAAAAGAAG CTGGGGACAA CTCTGTGAAA AACAAATCAA AACCAATAAC TGGGAGCAGT 
GGGGCCTCTG CTAGCTCTCA AAAGTCTCCT GGTCTCATCT ATAGGAGTGC TAAAAAATCC 
AATACATCCA ATGAAGAATT TAAGTTGCTG CTTTTGAAAA AGGGCAGCAG GTCAGATACC 
AGCTACCGCA TGTCTGCTAC TGAGATTCTG AAGAGTCCAG TAACACCTAA ATCCCTTGGT 
GATCTAGCAC TTGACTCCTC ATCAAGCTCT GAAGAGGGTG GTCAGTTATC ACCTGGAAGT 
GATGCATTTT CGCCTTTATC CCCTTGTTCT CCTCGTGTTA TATCTGAGGG GTTTTCTCCA 
AAAAGTGGAA CTATGTCTGC TGCCTCAAGA GTGGGAAGGT CCAGAGTGCC TCCTGCTGCA 
AGCAGCAGCC GTTACAGTGT CAGGTGCCGT TTTTACAATG CTCCAATGCA GGCCATATCA 
GAAGGAGAAA CAGAAAACTC GGATGGTAGC CCTCATGATG ACAGGTCCTC TCAGAGCTCA 
ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934404.2
CDS	441..5126
Translation	MPFAKRIVEPQWLCRHAITLQEGSCCRDQGEEHRQQHPRGNSREELSGVVAPSVQVPSDERETVPGDGKDKDVAPLDLGAFNNVALSRILRQLSDMARHACSLFHEIEVDIQVIGRRVRTLQGKISNVQQVVTSLDPKQETVPVSNLDEESKLEVYYKANWHQQRNIFLPNTRPPCVEELHRHAKQSLRSIRREQRIRADNRERRFQGSIFTAPPLPTYPAVHSLKRREVKEHHIVQFNRTRSHSPTECCHLTPWSRKAHPPQEEDDITLGQRPKNPIPNVPSTLDKQTNWNKALPLPTPEEKMKQESQVITSCIIPINVTGVGFDREASIRCSLVHSQSVLQRRRKLRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPELASSANRRSGTRDTECQTDDILITAPSRRRIRAQRGQGVAASLSHSAGNIAALTESGDQMFKTTVSSRIRSRSLPREGVRASNDHDCCTQSSTYEEDCYMPCTERTLKKDKDRLTSQESPDHQPLGLSYSQHRHSPPLNLNERGRSRLSRMADSGSCDISSNSDTFGSPVHSISAAGVLLSTHIDQKDDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNTAPHAGEDSNVFMIEQYSDDHIDSIRGHRASSFTSTVADLLDDPNNSNTSDSEWNYLHHHHDASCHQDFSPPHLKTDRLACPSFTSMGTYDSFLEKPASEKADSVSYYSVDTEGYYTSMHFDCGLNTSRSYICNYAPTGSESDQGPGVSSVNTDNPWQCYMDQGNEGNQNISFKKPKAKPTPPKRCSSLRKSDNNTDIPEKKEPKIVCGQQVNHSSREMKLPLEISNVPLRVENFATQHQQEIPWANQDDTELKNTQFLTTDITSFKDESGEQSHYADLWLLNDLKTSDPYRSLSNSSTATGTTVIECIKSPESSESQTSQSESRATSPSLPSVENEFKLSSPEKLAGLASPSSGYSSQSETPTSSFPIPFFSGPLSPGGSKRKPKVPERKSSLQHPSAKCGRELELPTIPPTHLDLSALHVLHKPLPYRPQMHAFSHNNQNMSEDQIDSNSSPALAITPSVLKSVHLRSVNKQHKKSKQRETCNLVCLQDPTITVDTYTPEKAIVTSARKSILRQFSTEETILPYKDSSPVDTSPESMFFDKCALFTGHGIYREEKGTNIFGVTKEPWTNEIVQETVFLVQPETTTTTFQEYYEKKNCGHAQDDQLKMTPDLHIQDDVMTIPEPVTGTTTGYTLQDNAAGTSLMPNYEPDLSNNDKVPGNTLSFESELPTVYSLSDECPEQESESVSGIPTKSASADKINDSSDNMEDTFCKESSQSEDYLISPMSEDSADTDDVFVSPNKPRTTEDLFAAIHRSKRKVLGRKEAGDNSVKNKSKPITGSSGASASSQKSPGLIYRSAKKSNTSNEEFKLLLLKKGSRSDTSYRMSATEILKSPVTPKSLGDLALDSSSSSEEGGQLSPGSDAFSPLSPCSPRVISEGFSPKSGTMSAASRVGRSRVPPAASSSRYSVRCRFYNAPMQAISEGETENSDGSPHDDRSSQSSM

Target ORF information:

RefSeq Version	XM_002934358.5
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934358.5

ORF Insert Sequence:

ATGCCCTTTG CCAAGAGGAT TGTTGAGCCC CAGTGGCTGT GCAGGCATGC CATCACCCTT 
CAAGAGGGTA GCTGCTGCCG GGATCAGGGA GAAGAGCACA GGCAGCAGCA CCCCCGGGGC 
AACTCCAGGG AAGAATTGTC AGGGGTGGTG GCACCTTCAG TGCAGGTGCC CTCTGATGAA 
AGGGAAACAG TGCCCGGTGA TGGAAAAGAT AAAGATGTGG CGCCACTGGA TTTGGGCGCA 
TTCAACAACG TCGCCTTGTC ACGGATTTTG CGTCAGTTGT CGGACATGGC CCGGCATGCC 
TGCTCCCTTT TCCATGAGAT TGAGGTGGAT ATACAGGTGA TCGGTCGGCG GGTGAGAACA 
TTGCAAGGGA AAATCAGCAA TGTCCAGCAG GTTGTGACCA GTCTGGATCC AAAACAGGAG 
ACAGTCCCGG TCTCAAACCT GGATGAAGAA AGCAAACTGG AAGTGTACTA CAAGGCCAAC 
TGGCACCAGC AGAGGAATAT TTTCCTGCCA AACACGAGGC CCCCGTGTGT GGAAGAGTTG 
CACCGCCATG CCAAGCAGAG TCTGCGTTCA ATACGGAGAG AGCAACGGAT CCGGGCAGAT 
AATAGAGAAA GAAGGTTTCA GGGCTCGATA TTTACAGCCC CACCGCTTCC CACATACCCA 
GCAGTTCACA GTTTGAAGAG ACGAGAAGTC AAAGAGCACC ATATTGTACA ATTTAACAGA 
ACCCGTTCGC ACTCCCCCAC TGAATGTTGC CATTTGACCC CATGGAGTAG AAAGGCTCAT 
CCACCTCAGG AGGAAGATGA TATCACTTTA GGACAAAGGC CGAAAAACCC TATTCCCAAT 
GTTCCATCTA CACTGGATAA GCAAACCAAC TGGAACAAGG CTTTACCTCT CCCCACTCCT 
GAGGAGAAGA TGAAACAGGA GTCCCAAGTG ATTACTTCTT GCATTATACC CATCAATGTT 
ACTGGGGTTG GATTTGATAG AGAGGCGAGC ATACGCTGTT CACTTGTTCA TTCACAATCT 
GTACTGCAGC GTAGACGGAA ACTGAGAAGA AGGAAAACTA TATCAGGAAT TCCCAGAAGA 
GTTCAACAAG AAATAGATTC TGATGAATCC CCAGTTGCAA GAGAGCGTAA TGTTATAGTT 
CACACCAACC CTGAACTTGC CAGCTCTGCA AACAGGAGAT CTGGTACACG TGATACAGAG 
TGCCAAACAG ATGATATACT CATTACTGCT CCATCTCGTA GAAGAATTAG AGCTCAAAGG 
GGCCAGGGAG TTGCAGCATC TCTCTCTCAT TCTGCTGGTA ACATTGCAGC CCTAACAGAG 
AGTGGTGACC AGATGTTTAA AACCACAGTA TCCAGTCGCA TTCGATCAAG GAGTCTTCCT 
AGAGAAGGTG TTAGAGCAAG CAATGATCAT GATTGTTGCA CACAGTCATC AACATATGAA 
GAAGACTGCT ATATGCCCTG CACTGAGAGG ACACTAAAGA AGGATAAAGA CAGATTAACT 
AGTCAGGAGT CTCCAGACCA CCAGCCATTA GGCTTATCCT ATTCCCAACA CCGACACAGC 
CCGCCGTTAA ATTTGAATGA AAGGGGGAGA TCAAGGCTAT CAAGAATGGC AGATTCAGGG 
AGCTGTGATA TCTCTTCAAA TTCAGATACA TTTGGAAGCC CTGTTCACTC AATTTCAGCA 
GCAGGTGTGT TGTTGAGCAC ACACATTGAT CAAAAAGATG ACCATCAGTC GTCTAGTGGA 
AACTGGAGCG GAAGTAGCTC AACCTGTCCC TCCCAGACAT CAGAAACAAT CCCTCCAGCT 
GCATCTCCTC CACTAACTGG TTCTTCCCAC TGTGATTCTG AATTATCCTT GAACACAGCT 
CCCCACGCTG GTGAAGATTC TAATGTTTTT ATGATTGAAC AATACAGTGA TGACCACATA 
GATAGCATAA GAGGTCACAG AGCCAGTTCC TTTACATCTA CAGTTGCTGA CCTGCTTGAC 
GATCCAAACA ACAGTAATAC TAGTGACAGC GAATGGAATT ACCTTCACCA TCACCATGAT 
GCATCATGTC ATCAAGATTT TAGTCCACCG CACTTAAAGA CAGATCGCCT AGCATGCCCT 
AGTTTTACCA GTATGGGCAC TTATGACAGT TTTCTTGAAA AACCTGCATC TGAAAAAGCA 
GATTCAGTTT CATATTATTC TGTAGACACT GAAGGATATT ATACCTCCAT GCATTTTGAC 
TGTGGTCTGA ATACTAGTAG GAGTTATATT TGTAACTATG CACCAACAGG GTCAGAAAGT 
GATCAAGGTC CTGGTGTTTC TTCTGTGAAT ACCGATAATC CTTGGCAGTG TTATATGGAT 
CAAGGCAATG AAGGAAACCA GAACATCTCA TTTAAGAAAC CAAAGGCAAA GCCTACACCA 
CCAAAACGTT GTTCATCTTT AAGGAAATCT GACAACAACA CAGATATTCC TGAGAAGAAA 
GAACCAAAGA TAGTATGTGG TCAGCAAGTG AATCACTCTT CCAGGGAAAT GAAGCTGCCA 
CTTGAGATTT CTAATGTTCC TTTAAGAGTG GAAAACTTTG CTACACAACA TCAACAGGAA 
ATACCATGGG CTAACCAGGA TGACACTGAG TTAAAGAACA CTCAATTTTT AACCACAGAT 
ATTACTTCTT TTAAAGATGA GAGTGGAGAG CAGTCTCACT ATGCAGATCT GTGGCTCCTC 
AATGACTTGA AAACAAGTGA TCCTTATAGG TCATTATCAA ACTCAAGCAC TGCCACAGGT 
ACTACAGTTA TAGAATGCAT TAAGTCACCA GAAAGTTCAG AGTCACAGAC ATCACAGTCT 
GAATCCAGAG CAACGTCGCC ATCTCTTCCT TCTGTGGAAA ATGAGTTCAA GTTGTCTTCT 
CCAGAAAAGC TTGCTGGCTT AGCATCACCA TCAAGTGGTT ACTCTAGCCA GTCAGAAACC 
CCTACATCCT CCTTTCCCAT ACCTTTCTTT TCTGGGCCCC TGTCACCTGG TGGTAGCAAA 
CGAAAGCCAA AAGTTCCAGA AAGAAAATCG TCCTTGCAAC ATCCTTCTGC AAAATGTGGA 
AGAGAACTGG AGCTTCCAAC TATACCTCCG ACCCATCTTG ACCTAAGTGC TCTTCATGTT 
TTACATAAAC CATTACCTTA CAGACCACAG ATGCATGCTT TCAGTCATAA TAATCAGAAT 
ATGTCAGAAG ATCAGATTGA CTCAAATTCT TCACCAGCTC TTGCCATAAC ACCATCTGTA 
CTTAAATCTG TCCATCTCAG ATCTGTCAAC AAGCAACATA AAAAGTCAAA GCAAAGAGAA 
ACCTGTAATC TTGTGTGCTT GCAGGATCCT ACAATAACAG TGGATACCTA TACCCCAGAA 
AAGGCAATTG TGACATCAGC CAGAAAATCA ATTTTGCGTC AGTTTTCTAC AGAGGAAACC 
ATCCTACCAT ATAAGGATTC CTCACCAGTT GACACCAGTC CAGAAAGTAT GTTTTTTGAC 
AAGTGTGCAC TATTCACTGG TCATGGAATA TACAGAGAAG AGAAAGGTAC AAATATATTT 
GGAGTCACTA AAGAACCTTG GACTAATGAA ATTGTGCAAG AAACTGTGTT TTTAGTACAG 
CCAGAGACCA CAACAACAAC TTTTCAAGAG TACTACGAGA AAAAGAACTG TGGCCATGCT 
CAAGATGATC AACTTAAGAT GACACCAGAC TTACATATAC AGGATGATGT AATGACTATA 
CCAGAGCCTG TAACAGGAAC AACTACTGGG TACACATTGC AAGACAATGC TGCTGGGACA 
TCTTTAATGC CTAACTATGA GCCAGACTTG AGCAACAATG ATAAAGTGCC TGGAAACACT 
CTCAGCTTTG AATCAGAGCT CCCCACTGTA TATTCACTCA GCGATGAATG TCCAGAGCAG 
GAATCTGAAT CTGTATCAGG AATTCCAACC AAAAGTGCCT CAGCGGACAA AATTAATGAT 
TCATCTGACA ACATGGAAGA CACCTTCTGC AAAGAATCAT CTCAGAGTGA GGACTACCTA 
ATTTCACCAA TGAGTGAGGA CTCGGCAGAC ACAGATGATG TGTTTGTGTC CCCTAACAAA 
CCTCGTACAA CAGAGGATCT TTTTGCAGCC ATTCACAGAT CCAAACGAAA AGTACTTGGA 
AGAAAAGAAG CTGGGGACAA CTCTGTGAAA AACAAATCAA AACCAATAAC TGGGAGCAGT 
GGGGCCTCTG CTAGCTCTCA AAAGTCTCCT GGTCTCATCT ATAGGAGTGC TAAAAAATCC 
AATACATCCA ATGAAGAATT TAAGTTGCTG CTTTTGAAAA AGGGCAGCAG GTCAGATACC 
AGCTACCGCA TGTCTGCTAC TGAGATTCTG AAGAGTCCAG TAACACCTAA ATCCCTTGGT 
GATCTAGCAC TTGACTCCTC ATCAAGCTCT GAAGAGGGTG GTCAGTTATC ACCTGGAAGT 
GATGCATTTT CGCCTTTATC CCCTTGTTCT CCTCGTGTTA TATCTGAGGG GTTTTCTCCA 
AAAAGTGGAA CTATGTCTGC TGCCTCAAGA GTGGGAAGGT CCAGAGTGCC TCCTGCTGCA 
AGCAGCAGCC GTTACAGTGT CAGGTGCCGT TTTTACAATG CTCCAATGCA GGCCATATCA 
GAAGGAGAAA CAGAAAACTC GGATGGTAGC CCTCATGATG ACAGGTCCTC TCAGAGCTCA 
ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhs ( XM_018091600.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017947089.1 Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X2, mRNA.

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CloneID	OXa52396
Clone ID Related Accession (Same CDS sequence)	XM_018091600.2
Accession Version	XM_018091600.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4623bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Nance-Horan syndrome protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018091600.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTTG CCAAGAGGAT TGTTGAGCCC CAGTGGCTGT GCAGGCATGC CATCACCCTT 
CAAGAGGGTA GCTGCTGCCG GGATCAGGGA GAAGAGCACA GGCAGCAGCA CCCCCGGGGC 
AACTCCAGGG AAGAATTGTC AGGGGTGGTG GCACCTTCAG TGCAGGTGCC CTCTGATGAA 
AGGGAAACAG TGCCCGGTGA TGGAAAAGAT AAAGATGTGG CGCCACTGGA TTTGGGCGCA 
TTCAACAACG TCGCCTTGTC ACGGATTTTG CGTCAGTTGT CGGACATGGC CCGGCATGCC 
TGCTCCCTTT TCCATGAGAT TGAGGTGGAT ATACAGGTGA TCGGTCGGCG GGTGAGAACA 
TTGCAAGGGA AAATCAGCAA TGTCCAGCAG GTTGTGACCA GTCTGGATCC AAAACAGGAG 
ACAGTCCCGG TCTCAAACCT GGATGAAGAA AGCAAACTGG AAGTGTACTA CAAGGCCAAC 
TGGCACCAGC AGAGGAATAT TTTCCTGCCA AACACGAGGC CCCCGTGTGT GGAAGAGTTG 
CACCGCCATG CCAAGCAGAG TCTGCGTTCA ATACGGAGAG AGCAACGGAT CCGGGCAGAT 
AATAGAGAAA GAAGGTTTCA GGGCTCGATA TTTACAGCCC CACCGCTTCC CACATACCCA 
GCAGTTCACA GTTTGAAGAG ACGAGAAGTC AAAGAGCACC ATATTGTACA AGCTCATCCA 
CCTCAGGAGG AAGATGATAT CACTTTAGGA CAAAGGCCGA AAAACCCTAT TCCCAATGTT 
CCATCTACAC TGGATAAGCA AACCAACTGG AACAAGGCTT TACCTCTCCC CACTCCTGAG 
GAGAAGATGA AACAGGAGTC CCAAGTGATT ACTTCTTGCA TTATACCCAT CAATGTTACT 
GGGGTTGGAT TTGATAGAGA GGCGAGCATA CGCTGTTCAC TTGTTCATTC ACAATCTGTA 
CTGCAGCGTA GACGGAAACT GAGAAGAAGG AAAACTATAT CAGGAATTCC CAGAAGAGTT 
CAACAAGAAA TAGATTCTGA TGAATCCCCA GTTGCAAGAG AGCGTAATGT TATAGTTCAC 
ACCAACCCTG AACTTGCCAG CTCTGCAAAC AGGAGATCTG GTACACGTGA TACAGAGTGC 
CAAACAGATG ATATACTCAT TACTGCTCCA TCTCGTAGAA GAATTAGAGC TCAAAGGGGC 
CAGGGAGTTG CAGCATCTCT CTCTCATTCT GCTGGTAACA TTGCAGCCCT AACAGAGAGT 
GGTGACCAGA TGTTTAAAAC CACAGTATCC AGTCGCATTC GATCAAGGAG TCTTCCTAGA 
GAAGGTGTTA GAGCAAGCAA TGATCATGAT TGTTGCACAC AGTCATCAAC ATATGAAGAA 
GACTGCTATA TGCCCTGCAC TGAGAGGACA CTAAAGAAGG ATAAAGACAG ATTAACTAGT 
CAGGAGTCTC CAGACCACCA GCCATTAGGC TTATCCTATT CCCAACACCG ACACAGCCCG 
CCGTTAAATT TGAATGAAAG GGGGAGATCA AGGCTATCAA GAATGGCAGA TTCAGGGAGC 
TGTGATATCT CTTCAAATTC AGATACATTT GGAAGCCCTG TTCACTCAAT TTCAGCAGCA 
GGTGTGTTGT TGAGCACACA CATTGATCAA AAAGATGACC ATCAGTCGTC TAGTGGAAAC 
TGGAGCGGAA GTAGCTCAAC CTGTCCCTCC CAGACATCAG AAACAATCCC TCCAGCTGCA 
TCTCCTCCAC TAACTGGTTC TTCCCACTGT GATTCTGAAT TATCCTTGAA CACAGCTCCC 
CACGCTGGTG AAGATTCTAA TGTTTTTATG ATTGAACAAT ACAGTGATGA CCACATAGAT 
AGCATAAGAG GTCACAGAGC CAGTTCCTTT ACATCTACAG TTGCTGACCT GCTTGACGAT 
CCAAACAACA GTAATACTAG TGACAGCGAA TGGAATTACC TTCACCATCA CCATGATGCA 
TCATGTCATC AAGATTTTAG TCCACCGCAC TTAAAGACAG ATCGCCTAGC ATGCCCTAGT 
TTTACCAGTA TGGGCACTTA TGACAGTTTT CTTGAAAAAC CTGCATCTGA AAAAGCAGAT 
TCAGTTTCAT ATTATTCTGT AGACACTGAA GGATATTATA CCTCCATGCA TTTTGACTGT 
GGTCTGAATA CTAGTAGGAG TTATATTTGT AACTATGCAC CAACAGGGTC AGAAAGTGAT 
CAAGGTCCTG GTGTTTCTTC TGTGAATACC GATAATCCTT GGCAGTGTTA TATGGATCAA 
GGCAATGAAG GAAACCAGAA CATCTCATTT AAGAAACCAA AGGCAAAGCC TACACCACCA 
AAACGTTGTT CATCTTTAAG GAAATCTGAC AACAACACAG ATATTCCTGA GAAGAAAGAA 
CCAAAGATAG TATGTGGTCA GCAAGTGAAT CACTCTTCCA GGGAAATGAA GCTGCCACTT 
GAGATTTCTA ATGTTCCTTT AAGAGTGGAA AACTTTGCTA CACAACATCA ACAGGAAATA 
CCATGGGCTA ACCAGGATGA CACTGAGTTA AAGAACACTC AATTTTTAAC CACAGATATT 
ACTTCTTTTA AAGATGAGAG TGGAGAGCAG TCTCACTATG CAGATCTGTG GCTCCTCAAT 
GACTTGAAAA CAAGTGATCC TTATAGGTCA TTATCAAACT CAAGCACTGC CACAGGTACT 
ACAGTTATAG AATGCATTAA GTCACCAGAA AGTTCAGAGT CACAGACATC ACAGTCTGAA 
TCCAGAGCAA CGTCGCCATC TCTTCCTTCT GTGGAAAATG AGTTCAAGTT GTCTTCTCCA 
GAAAAGCTTG CTGGCTTAGC ATCACCATCA AGTGGTTACT CTAGCCAGTC AGAAACCCCT 
ACATCCTCCT TTCCCATACC TTTCTTTTCT GGGCCCCTGT CACCTGGTGG TAGCAAACGA 
AAGCCAAAAG TTCCAGAAAG AAAATCGTCC TTGCAACATC CTTCTGCAAA ATGTGGAAGA 
GAACTGGAGC TTCCAACTAT ACCTCCGACC CATCTTGACC TAAGTGCTCT TCATGTTTTA 
CATAAACCAT TACCTTACAG ACCACAGATG CATGCTTTCA GTCATAATAA TCAGAATATG 
TCAGAAGATC AGATTGACTC AAATTCTTCA CCAGCTCTTG CCATAACACC ATCTGTACTT 
AAATCTGTCC ATCTCAGATC TGTCAACAAG CAACATAAAA AGTCAAAGCA AAGAGAAACC 
TGTAATCTTG TGTGCTTGCA GGATCCTACA ATAACAGTGG ATACCTATAC CCCAGAAAAG 
GCAATTGTGA CATCAGCCAG AAAATCAATT TTGCGTCAGT TTTCTACAGA GGAAACCATC 
CTACCATATA AGGATTCCTC ACCAGTTGAC ACCAGTCCAG AAAGTATGTT TTTTGACAAG 
TGTGCACTAT TCACTGGTCA TGGAATATAC AGAGAAGAGA AAGGTACAAA TATATTTGGA 
GTCACTAAAG AACCTTGGAC TAATGAAATT GTGCAAGAAA CTGTGTTTTT AGTACAGCCA 
GAGACCACAA CAACAACTTT TCAAGAGTAC TACGAGAAAA AGAACTGTGG CCATGCTCAA 
GATGATCAAC TTAAGATGAC ACCAGACTTA CATATACAGG ATGATGTAAT GACTATACCA 
GAGCCTGTAA CAGGAACAAC TACTGGGTAC ACATTGCAAG ACAATGCTGC TGGGACATCT 
TTAATGCCTA ACTATGAGCC AGACTTGAGC AACAATGATA AAGTGCCTGG AAACACTCTC 
AGCTTTGAAT CAGAGCTCCC CACTGTATAT TCACTCAGCG ATGAATGTCC AGAGCAGGAA 
TCTGAATCTG TATCAGGAAT TCCAACCAAA AGTGCCTCAG CGGACAAAAT TAATGATTCA 
TCTGACAACA TGGAAGACAC CTTCTGCAAA GAATCATCTC AGAGTGAGGA CTACCTAATT 
TCACCAATGA GTGAGGACTC GGCAGACACA GATGATGTGT TTGTGTCCCC TAACAAACCT 
CGTACAACAG AGGATCTTTT TGCAGCCATT CACAGATCCA AACGAAAAGT ACTTGGAAGA 
AAAGAAGCTG GGGACAACTC TGTGAAAAAC AAATCAAAAC CAATAACTGG GAGCAGTGGG 
GCCTCTGCTA GCTCTCAAAA GTCTCCTGGT CTCATCTATA GGAGTGCTAA AAAATCCAAT 
ACATCCAATG AAGAATTTAA GTTGCTGCTT TTGAAAAAGG GCAGCAGGTC AGATACCAGC 
TACCGCATGT CTGCTACTGA GATTCTGAAG AGTCCAGTAA CACCTAAATC CCTTGGTGAT 
CTAGCACTTG ACTCCTCATC AAGCTCTGAA GAGGGTGGTC AGTTATCACC TGGAAGTGAT 
GCATTTTCGC CTTTATCCCC TTGTTCTCCT CGTGTTATAT CTGAGGGGTT TTCTCCAAAA 
AGTGGAACTA TGTCTGCTGC CTCAAGAGTG GGAAGGTCCA GAGTGCCTCC TGCTGCAAGC 
AGCAGCCGTT ACAGTGTCAG GTGCCGTTTT TACAATGCTC CAATGCAGGC CATATCAGAA 
GGAGAAACAG AAAACTCGGA TGGTAGCCCT CATGATGACA GGTCCTCTCA GAGCTCAATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017947089.1
CDS	442..5064
Translation	MPFAKRIVEPQWLCRHAITLQEGSCCRDQGEEHRQQHPRGNSREELSGVVAPSVQVPSDERETVPGDGKDKDVAPLDLGAFNNVALSRILRQLSDMARHACSLFHEIEVDIQVIGRRVRTLQGKISNVQQVVTSLDPKQETVPVSNLDEESKLEVYYKANWHQQRNIFLPNTRPPCVEELHRHAKQSLRSIRREQRIRADNRERRFQGSIFTAPPLPTYPAVHSLKRREVKEHHIVQAHPPQEEDDITLGQRPKNPIPNVPSTLDKQTNWNKALPLPTPEEKMKQESQVITSCIIPINVTGVGFDREASIRCSLVHSQSVLQRRRKLRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPELASSANRRSGTRDTECQTDDILITAPSRRRIRAQRGQGVAASLSHSAGNIAALTESGDQMFKTTVSSRIRSRSLPREGVRASNDHDCCTQSSTYEEDCYMPCTERTLKKDKDRLTSQESPDHQPLGLSYSQHRHSPPLNLNERGRSRLSRMADSGSCDISSNSDTFGSPVHSISAAGVLLSTHIDQKDDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNTAPHAGEDSNVFMIEQYSDDHIDSIRGHRASSFTSTVADLLDDPNNSNTSDSEWNYLHHHHDASCHQDFSPPHLKTDRLACPSFTSMGTYDSFLEKPASEKADSVSYYSVDTEGYYTSMHFDCGLNTSRSYICNYAPTGSESDQGPGVSSVNTDNPWQCYMDQGNEGNQNISFKKPKAKPTPPKRCSSLRKSDNNTDIPEKKEPKIVCGQQVNHSSREMKLPLEISNVPLRVENFATQHQQEIPWANQDDTELKNTQFLTTDITSFKDESGEQSHYADLWLLNDLKTSDPYRSLSNSSTATGTTVIECIKSPESSESQTSQSESRATSPSLPSVENEFKLSSPEKLAGLASPSSGYSSQSETPTSSFPIPFFSGPLSPGGSKRKPKVPERKSSLQHPSAKCGRELELPTIPPTHLDLSALHVLHKPLPYRPQMHAFSHNNQNMSEDQIDSNSSPALAITPSVLKSVHLRSVNKQHKKSKQRETCNLVCLQDPTITVDTYTPEKAIVTSARKSILRQFSTEETILPYKDSSPVDTSPESMFFDKCALFTGHGIYREEKGTNIFGVTKEPWTNEIVQETVFLVQPETTTTTFQEYYEKKNCGHAQDDQLKMTPDLHIQDDVMTIPEPVTGTTTGYTLQDNAAGTSLMPNYEPDLSNNDKVPGNTLSFESELPTVYSLSDECPEQESESVSGIPTKSASADKINDSSDNMEDTFCKESSQSEDYLISPMSEDSADTDDVFVSPNKPRTTEDLFAAIHRSKRKVLGRKEAGDNSVKNKSKPITGSSGASASSQKSPGLIYRSAKKSNTSNEEFKLLLLKKGSRSDTSYRMSATEILKSPVTPKSLGDLALDSSSSSEEGGQLSPGSDAFSPLSPCSPRVISEGFSPKSGTMSAASRVGRSRVPPAASSSRYSVRCRFYNAPMQAISEGETENSDGSPHDDRSSQSSM

Target ORF information:

RefSeq Version	XM_018091600.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018091600.2

ORF Insert Sequence:

ATGCCCTTTG CCAAGAGGAT TGTTGAGCCC CAGTGGCTGT GCAGGCATGC CATCACCCTT 
CAAGAGGGTA GCTGCTGCCG GGATCAGGGA GAAGAGCACA GGCAGCAGCA CCCCCGGGGC 
AACTCCAGGG AAGAATTGTC AGGGGTGGTG GCACCTTCAG TGCAGGTGCC CTCTGATGAA 
AGGGAAACAG TGCCCGGTGA TGGAAAAGAT AAAGATGTGG CGCCACTGGA TTTGGGCGCA 
TTCAACAACG TCGCCTTGTC ACGGATTTTG CGTCAGTTGT CGGACATGGC CCGGCATGCC 
TGCTCCCTTT TCCATGAGAT TGAGGTGGAT ATACAGGTGA TCGGTCGGCG GGTGAGAACA 
TTGCAAGGGA AAATCAGCAA TGTCCAGCAG GTTGTGACCA GTCTGGATCC AAAACAGGAG 
ACAGTCCCGG TCTCAAACCT GGATGAAGAA AGCAAACTGG AAGTGTACTA CAAGGCCAAC 
TGGCACCAGC AGAGGAATAT TTTCCTGCCA AACACGAGGC CCCCGTGTGT GGAAGAGTTG 
CACCGCCATG CCAAGCAGAG TCTGCGTTCA ATACGGAGAG AGCAACGGAT CCGGGCAGAT 
AATAGAGAAA GAAGGTTTCA GGGCTCGATA TTTACAGCCC CACCGCTTCC CACATACCCA 
GCAGTTCACA GTTTGAAGAG ACGAGAAGTC AAAGAGCACC ATATTGTACA AGCTCATCCA 
CCTCAGGAGG AAGATGATAT CACTTTAGGA CAAAGGCCGA AAAACCCTAT TCCCAATGTT 
CCATCTACAC TGGATAAGCA AACCAACTGG AACAAGGCTT TACCTCTCCC CACTCCTGAG 
GAGAAGATGA AACAGGAGTC CCAAGTGATT ACTTCTTGCA TTATACCCAT CAATGTTACT 
GGGGTTGGAT TTGATAGAGA GGCGAGCATA CGCTGTTCAC TTGTTCATTC ACAATCTGTA 
CTGCAGCGTA GACGGAAACT GAGAAGAAGG AAAACTATAT CAGGAATTCC CAGAAGAGTT 
CAACAAGAAA TAGATTCTGA TGAATCCCCA GTTGCAAGAG AGCGTAATGT TATAGTTCAC 
ACCAACCCTG AACTTGCCAG CTCTGCAAAC AGGAGATCTG GTACACGTGA TACAGAGTGC 
CAAACAGATG ATATACTCAT TACTGCTCCA TCTCGTAGAA GAATTAGAGC TCAAAGGGGC 
CAGGGAGTTG CAGCATCTCT CTCTCATTCT GCTGGTAACA TTGCAGCCCT AACAGAGAGT 
GGTGACCAGA TGTTTAAAAC CACAGTATCC AGTCGCATTC GATCAAGGAG TCTTCCTAGA 
GAAGGTGTTA GAGCAAGCAA TGATCATGAT TGTTGCACAC AGTCATCAAC ATATGAAGAA 
GACTGCTATA TGCCCTGCAC TGAGAGGACA CTAAAGAAGG ATAAAGACAG ATTAACTAGT 
CAGGAGTCTC CAGACCACCA GCCATTAGGC TTATCCTATT CCCAACACCG ACACAGCCCG 
CCGTTAAATT TGAATGAAAG GGGGAGATCA AGGCTATCAA GAATGGCAGA TTCAGGGAGC 
TGTGATATCT CTTCAAATTC AGATACATTT GGAAGCCCTG TTCACTCAAT TTCAGCAGCA 
GGTGTGTTGT TGAGCACACA CATTGATCAA AAAGATGACC ATCAGTCGTC TAGTGGAAAC 
TGGAGCGGAA GTAGCTCAAC CTGTCCCTCC CAGACATCAG AAACAATCCC TCCAGCTGCA 
TCTCCTCCAC TAACTGGTTC TTCCCACTGT GATTCTGAAT TATCCTTGAA CACAGCTCCC 
CACGCTGGTG AAGATTCTAA TGTTTTTATG ATTGAACAAT ACAGTGATGA CCACATAGAT 
AGCATAAGAG GTCACAGAGC CAGTTCCTTT ACATCTACAG TTGCTGACCT GCTTGACGAT 
CCAAACAACA GTAATACTAG TGACAGCGAA TGGAATTACC TTCACCATCA CCATGATGCA 
TCATGTCATC AAGATTTTAG TCCACCGCAC TTAAAGACAG ATCGCCTAGC ATGCCCTAGT 
TTTACCAGTA TGGGCACTTA TGACAGTTTT CTTGAAAAAC CTGCATCTGA AAAAGCAGAT 
TCAGTTTCAT ATTATTCTGT AGACACTGAA GGATATTATA CCTCCATGCA TTTTGACTGT 
GGTCTGAATA CTAGTAGGAG TTATATTTGT AACTATGCAC CAACAGGGTC AGAAAGTGAT 
CAAGGTCCTG GTGTTTCTTC TGTGAATACC GATAATCCTT GGCAGTGTTA TATGGATCAA 
GGCAATGAAG GAAACCAGAA CATCTCATTT AAGAAACCAA AGGCAAAGCC TACACCACCA 
AAACGTTGTT CATCTTTAAG GAAATCTGAC AACAACACAG ATATTCCTGA GAAGAAAGAA 
CCAAAGATAG TATGTGGTCA GCAAGTGAAT CACTCTTCCA GGGAAATGAA GCTGCCACTT 
GAGATTTCTA ATGTTCCTTT AAGAGTGGAA AACTTTGCTA CACAACATCA ACAGGAAATA 
CCATGGGCTA ACCAGGATGA CACTGAGTTA AAGAACACTC AATTTTTAAC CACAGATATT 
ACTTCTTTTA AAGATGAGAG TGGAGAGCAG TCTCACTATG CAGATCTGTG GCTCCTCAAT 
GACTTGAAAA CAAGTGATCC TTATAGGTCA TTATCAAACT CAAGCACTGC CACAGGTACT 
ACAGTTATAG AATGCATTAA GTCACCAGAA AGTTCAGAGT CACAGACATC ACAGTCTGAA 
TCCAGAGCAA CGTCGCCATC TCTTCCTTCT GTGGAAAATG AGTTCAAGTT GTCTTCTCCA 
GAAAAGCTTG CTGGCTTAGC ATCACCATCA AGTGGTTACT CTAGCCAGTC AGAAACCCCT 
ACATCCTCCT TTCCCATACC TTTCTTTTCT GGGCCCCTGT CACCTGGTGG TAGCAAACGA 
AAGCCAAAAG TTCCAGAAAG AAAATCGTCC TTGCAACATC CTTCTGCAAA ATGTGGAAGA 
GAACTGGAGC TTCCAACTAT ACCTCCGACC CATCTTGACC TAAGTGCTCT TCATGTTTTA 
CATAAACCAT TACCTTACAG ACCACAGATG CATGCTTTCA GTCATAATAA TCAGAATATG 
TCAGAAGATC AGATTGACTC AAATTCTTCA CCAGCTCTTG CCATAACACC ATCTGTACTT 
AAATCTGTCC ATCTCAGATC TGTCAACAAG CAACATAAAA AGTCAAAGCA AAGAGAAACC 
TGTAATCTTG TGTGCTTGCA GGATCCTACA ATAACAGTGG ATACCTATAC CCCAGAAAAG 
GCAATTGTGA CATCAGCCAG AAAATCAATT TTGCGTCAGT TTTCTACAGA GGAAACCATC 
CTACCATATA AGGATTCCTC ACCAGTTGAC ACCAGTCCAG AAAGTATGTT TTTTGACAAG 
TGTGCACTAT TCACTGGTCA TGGAATATAC AGAGAAGAGA AAGGTACAAA TATATTTGGA 
GTCACTAAAG AACCTTGGAC TAATGAAATT GTGCAAGAAA CTGTGTTTTT AGTACAGCCA 
GAGACCACAA CAACAACTTT TCAAGAGTAC TACGAGAAAA AGAACTGTGG CCATGCTCAA 
GATGATCAAC TTAAGATGAC ACCAGACTTA CATATACAGG ATGATGTAAT GACTATACCA 
GAGCCTGTAA CAGGAACAAC TACTGGGTAC ACATTGCAAG ACAATGCTGC TGGGACATCT 
TTAATGCCTA ACTATGAGCC AGACTTGAGC AACAATGATA AAGTGCCTGG AAACACTCTC 
AGCTTTGAAT CAGAGCTCCC CACTGTATAT TCACTCAGCG ATGAATGTCC AGAGCAGGAA 
TCTGAATCTG TATCAGGAAT TCCAACCAAA AGTGCCTCAG CGGACAAAAT TAATGATTCA 
TCTGACAACA TGGAAGACAC CTTCTGCAAA GAATCATCTC AGAGTGAGGA CTACCTAATT 
TCACCAATGA GTGAGGACTC GGCAGACACA GATGATGTGT TTGTGTCCCC TAACAAACCT 
CGTACAACAG AGGATCTTTT TGCAGCCATT CACAGATCCA AACGAAAAGT ACTTGGAAGA 
AAAGAAGCTG GGGACAACTC TGTGAAAAAC AAATCAAAAC CAATAACTGG GAGCAGTGGG 
GCCTCTGCTA GCTCTCAAAA GTCTCCTGGT CTCATCTATA GGAGTGCTAA AAAATCCAAT 
ACATCCAATG AAGAATTTAA GTTGCTGCTT TTGAAAAAGG GCAGCAGGTC AGATACCAGC 
TACCGCATGT CTGCTACTGA GATTCTGAAG AGTCCAGTAA CACCTAAATC CCTTGGTGAT 
CTAGCACTTG ACTCCTCATC AAGCTCTGAA GAGGGTGGTC AGTTATCACC TGGAAGTGAT 
GCATTTTCGC CTTTATCCCC TTGTTCTCCT CGTGTTATAT CTGAGGGGTT TTCTCCAAAA 
AGTGGAACTA TGTCTGCTGC CTCAAGAGTG GGAAGGTCCA GAGTGCCTCC TGCTGCAAGC 
AGCAGCCGTT ACAGTGTCAG GTGCCGTTTT TACAATGCTC CAATGCAGGC CATATCAGAA 
GGAGAAACAG AAAACTCGGA TGGTAGCCCT CATGATGACA GGTCCTCTCA GAGCTCAATG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhs ( XM_002934358.4 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_002934404.2 Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X1, mRNA.

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CloneID	OXa05595
Clone ID Related Accession (Same CDS sequence)	XM_002934358.4
Accession Version	XM_002934358.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4686bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	Nance-Horan syndrome protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030678.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002934358.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTTG CCAAGAGGAT TGTTGAGCCC CAGTGGCTGT GCAGGCATGC CATCACCCTT 
CAAGAGGGTA GCTGCTGCCG GGATCAGGGA GAAGAGCACA GGCAGCAGCA CCCCCGGGGC 
AACTCCAGGG AAGAATTGTC AGGGGTGGTG GCACCTTCAG TGCAGGTGCC CTCTGATGAA 
AGGGAAACAG TGCCCGGTGA TGGAAAAGAT AAAGATGTGG CGCCACTGGA TTTGGGCGCA 
TTCAACAACG TCGCCTTGTC ACGGATTTTG CGTCAGTTGT CGGACATGGC CCGGCATGCC 
TGCTCCCTTT TCCATGAGAT TGAGGTGGAT ATACAGGTGA TCGGTCGGCG GGTGAGAACA 
TTGCAAGGGA AAATCAGCAA TGTCCAGCAG GTTGTGACCA GTCTGGATCC AAAACAGGAG 
ACAGTCCCGG TCTCAAACCT GGATGAAGAA AGCAAACTGG AAGTGTACTA CAAGGCCAAC 
TGGCACCAGC AGAGGAATAT TTTCCTGCCA AACACGAGGC CCCCGTGTGT GGAAGAGTTG 
CACCGCCATG CCAAGCAGAG TCTGCGTTCA ATACGGAGAG AGCAACGGAT CCGGGCAGAT 
AATAGAGAAA GAAGGTTTCA GGGCTCGATA TTTACAGCCC CACCGCTTCC CACATACCCA 
GCAGTTCACA GTCTGAAGAG ACGAGAAGTC AAAGAGCACC ATATTGTACA ATTTAACAGA 
ACCCGTTCGC ACTCCCCCAC TGAATGTTGC CATTTGACCC CATGGAGTAG AAAGGCTCAT 
CCACCTCAGG AGGAAGATGA TATCACTTTA GGACAAAGGC CGAAAAACCC TATTCCCAAT 
GTTCCATCTA CACTGGATAA GCAAACCAAC TGGAACAAGG CTTTACCTCT CCCCACTCCT 
GAGGAGAAGA TGAAACAGGA GTCCCAAGTG ATTACTTCTT GCATTATACC CATCAATGTT 
ACTGGGGTTG GATTTGATAG AGAGGCGAGC ATACGCTGTT CACTTGTTCA TTCACAATCT 
GTACTGCAGC GTAGACGGAA ACTGAGAAGA AGGAAAACTA TATCAGGAAT TCCCAGAAGA 
GTTCAACAAG AAATAGATTC TGATGAATCC CCAGTTGCAA GAGAGCGTAA TGTTATAGTT 
CACACCAACC CTGAACTTGC CAGCTCTGCA AACAGGAGAT CTGGTACACG TGATACAGAG 
TGCCAAACAG ATGATATACT CATTACTGCT CCATCTCGTA GAAGAATTAG AGCTCAAAGG 
GGCCAGGGAG TTGCAGCATC TCTCTCTCAT TCTGCTGGTA ACATTGCAGC CCTAACAGAG 
AGTGGTGACC AGATGTTTAA AACCACAGTA TCCAGTCGCA TTCGATCAAG GAGTCTTCCT 
AGAGAAGGTG TTAGAGCAAG CAATGATCAT GATTGTTGCA CACAGTCATC AACATATGAA 
GAAGACTGCT ATATGCCCTG CACTGAGAGG ACACTAAAGA AGGATAAAGA CAGATTAACT 
AGTCAGGAGT CTCCAGACCA CCAGCCATTA GGCTTATCCT ATTCCCAACA CCGACACAGC 
CCGCCGTTAA ATTTGAATGA AAGGGGGAGA TCAAGGCTAT CAAGAATGGC AGATTCAGGG 
AGCTGTGATA TCTCTTCAAA TTCAGATACA TTTGGAAGCC CTGTTCACTC AATTTCAGCA 
GCAGGTGTGT TGTTGAGCAC ACACATTGAT CAAAAAGATG ACCATCAGTC GTCTAGTGGA 
AACTGGAGCG GAAGTAGCTC AACCTGTCCC TCCCAGACAT CAGAAACAAT CCCTCCAGCT 
GCATCTCCTC CACTAACTGG TTCTTCCCAC TGTGATTCTG AATTATCCTT GAACACAGCT 
CCCCACGCTG GTGAAGATTC TAATGTTTTT ATGATTGAAC AATACAGTGA TGACCACATA 
GATAGCATAA GAGGTCACAG AGCCAGTTCC TTTACATCTA CAGTTGCTGA CCTGCTTGAC 
GATCCAAACA ACAGTAATAC TAGTGACAGC GAATGGAATT ACCTTCACCA TCACCATGAT 
GCATCATGTC ATCAAGATTT TAGTCCACCG CACTTAAAGA CAGATCGCCT AGCATGCCCT 
AGTTTTACCA GTATGGGCAC TTATGACAGT TTTCTTGAAA AACCTGCATC TGAAAAAGCA 
GATTCAGTTT CATATTATTC TGTAGACACT GAAGGATATT ATACCTCCAT GCATTTTGAC 
TGTGGTCTGA ATACTAGTAG GAGTTATATT TGTAACTATG CACCAACAGG GTCAGAAAGT 
GATCAAGGTC CTGGTGTTTC TTCTGTGAAT ACCGATAATC CTTGGCAGTG TTATATGGAT 
CAAGGCAATG AAGGAAACCA GAACATCTCA TTTAAGAAAC CAAAGGCAAA GCCTACACCA 
CCAAAACGTT GTTCATCTTT AAGGAAATCT GACAACAACA CAGATATTCC TGAGAAGAAA 
GAACCAAAGA TAGTATGTGG TCAGCAAGTG AATCACTCTT CCAGGGAAAT GAAGCTGCCA 
CTTGAGATTT CTAATGTTCC TTTAAGAGTG GAAAACTTTG CTACACAACA TCAACAGGAA 
ATACCATGGG CTAACCAGGA TGACACTGAG TTAAAGAACA CTCAATTTTT AACCACAGAT 
ATTACTTCTT TTAAAGATGA GAGTGGAGAG CAGTCTCACT ATGCAGATCT GTGGCTCCTC 
AATGACTTGA AAACAAGTGA TCCTTATAGG TCATTATCAA ACTCAAGCAC TGCCACAGGT 
ACTACAGTTA TAGAATGCAT TAAGTCACCA GAAAGTTCAG AGTCACAGAC ATCACAGTCT 
GAATCCAGAG CAACGTCGCC ATCTCTTCCT TCTGTGGAAA ATGAGTTCAA GTTGTCTTCT 
CCAGAAAAGC TTGCTGGCTT AGCATCACCA TCAAGTGGTT ACTCTAGCCA GTCAGAAACC 
CCTACATCCT CCTTTCCCAT ACCTTTCTTT TCTGGGCCCC TGTCACCTGG TGGTAGCAAA 
CGAAAGCCAA AAGTTCCAGA AAGAAAATCG TCCTTGCAAC ATCCTTCTGC AAAATGTGGA 
AGAGAACTGG AGCTTCCAAC TATACCTCCG ACCCATCTTG ACCTAAGTGC TCTTCATGTT 
TTACATAAAC CATTACCTTA CAGACCACAG ATGCATGCTT TCAGTCATAA TAATCAGAAT 
ATGTCAGAAG ATCAGATTGA CTCAAATTCT TCACCAGCTC TTGCCATAAC ACCATCTGTA 
CTTAAATCTG TCCATCTCAG ATCTGTCAAC AAGCAACATA AAAAGTCAAA GCAAAGAGAA 
ACCTGTAATC TTGTGTGCTT GCAGGATCCT ACAATAACAG TGGATACCTA TACCCCAGAA 
AAGGCAATTG TGACATCAGC CAGAAAATCA ATTTTGCGTC AGTTTTCTAC AGAGGAAACC 
ATCCTACCAT ATAAGGATTC CTCACCAGTT GACACCAGTC CAGAAAGTAT GTTTTTTGAC 
AAGTGTGCAC TATTCACTGG TCATGGAATA TACAGAGAAG AGAAAGGTAC AAATATATTT 
GGAGTCACTA AAGAACCTTG GACTAATGAA ATTGTGCAAG AAACTGTGTT TTTAGTACAG 
CCAGAGACCA CAACAACAAC TTTTCAAGAG TACTACGAGA AAAAGAACTG TGGCCATGCT 
CAAGATGATC AACTTAAGAT GACACCAGAC TTACATATAC AGGATGATGT AATGACTATA 
CCAGAGCCTG TAACAGGAAC AACTACTGGG TACACATTGC AAGACAATGC TGCTGGGACA 
TCTTTAATGC CTAACTATGA GCCAGACTTG AGCAACAATG ATAAAGTGCC TGGAAACACT 
CTCAGCTTTG AATCAGAGCT CCCCACTGTA TATTCACTCA GCGATGAATG TCCAGAGCAG 
GAATCTGAAT CTGTATCAGG AATTCCAACC AAAAGTGCCT CAGCGGACAA AATTAATGAT 
TCATCTGACA ACATGGAAGA CACCTTCTGC AAAGAATCAT CTCAGAGTGA GGACTACCTA 
ATTTCACCAA TGAGTGAGGA CTCGGCAGAC ACAGATGATG TGTTTGTGTC CCCTAACAAA 
CCTCGTACAA CAGAGGATCT TTTTGCAGCC ATTCACAGAT CCAAACGAAA AGTACTTGGA 
AGAAAAGAAG CTGGGGACAA CTCTGTGAAA AACAAATCAA AACCAATAAC TGGGAGCAGT 
GGGGCCTCTG CTAGCTCTCA AAAGTCTCCT GGTCTCATCT ATAGGAGTGC TAAAAAATCC 
AATACATCCA ATGAAGAATT TAAGTTGCTG CTTTTGAAAA AGGGCAGCAG GTCAGATACC 
AGCTACCGCA TGTCTGCTAC TGAGATTCTG AAGAGTCCAG TAACACCTAA ATCCCTTGGT 
GATCTAGCAC TTGACTCCTC ATCAAGCTCT GAAGAGGGTG GTCAGTTATC ACCTGGAAGT 
GATGCATTTT CGCCTTTATC CCCTTGTTCT CCTCGTGTTA TATCTGAGGG GTTTTCTCCA 
AAAAGTGGAA CTATGTCTGC TGCCTCAAGA GTGGGAAGGT CCAGAGTGCC TCCTGCTGCA 
AGCAGCAGCC GTTACAGTGT CAGGTGCCGT TTTTACAATG CTCCAATGCA GGCCATATCA 
GAAGGAGAAA CAGAAAACTC GGATGGTAGC CCTCATGATG ACAGGTCCTC TCAGAGCTCA 
ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002934404.2
CDS	1825..6510
Translation	MPFAKRIVEPQWLCRHAITLQEGSCCRDQGEEHRQQHPRGNSREELSGVVAPSVQVPSDERETVPGDGKDKDVAPLDLGAFNNVALSRILRQLSDMARHACSLFHEIEVDIQVIGRRVRTLQGKISNVQQVVTSLDPKQETVPVSNLDEESKLEVYYKANWHQQRNIFLPNTRPPCVEELHRHAKQSLRSIRREQRIRADNRERRFQGSIFTAPPLPTYPAVHSLKRREVKEHHIVQFNRTRSHSPTECCHLTPWSRKAHPPQEEDDITLGQRPKNPIPNVPSTLDKQTNWNKALPLPTPEEKMKQESQVITSCIIPINVTGVGFDREASIRCSLVHSQSVLQRRRKLRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPELASSANRRSGTRDTECQTDDILITAPSRRRIRAQRGQGVAASLSHSAGNIAALTESGDQMFKTTVSSRIRSRSLPREGVRASNDHDCCTQSSTYEEDCYMPCTERTLKKDKDRLTSQESPDHQPLGLSYSQHRHSPPLNLNERGRSRLSRMADSGSCDISSNSDTFGSPVHSISAAGVLLSTHIDQKDDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNTAPHAGEDSNVFMIEQYSDDHIDSIRGHRASSFTSTVADLLDDPNNSNTSDSEWNYLHHHHDASCHQDFSPPHLKTDRLACPSFTSMGTYDSFLEKPASEKADSVSYYSVDTEGYYTSMHFDCGLNTSRSYICNYAPTGSESDQGPGVSSVNTDNPWQCYMDQGNEGNQNISFKKPKAKPTPPKRCSSLRKSDNNTDIPEKKEPKIVCGQQVNHSSREMKLPLEISNVPLRVENFATQHQQEIPWANQDDTELKNTQFLTTDITSFKDESGEQSHYADLWLLNDLKTSDPYRSLSNSSTATGTTVIECIKSPESSESQTSQSESRATSPSLPSVENEFKLSSPEKLAGLASPSSGYSSQSETPTSSFPIPFFSGPLSPGGSKRKPKVPERKSSLQHPSAKCGRELELPTIPPTHLDLSALHVLHKPLPYRPQMHAFSHNNQNMSEDQIDSNSSPALAITPSVLKSVHLRSVNKQHKKSKQRETCNLVCLQDPTITVDTYTPEKAIVTSARKSILRQFSTEETILPYKDSSPVDTSPESMFFDKCALFTGHGIYREEKGTNIFGVTKEPWTNEIVQETVFLVQPETTTTTFQEYYEKKNCGHAQDDQLKMTPDLHIQDDVMTIPEPVTGTTTGYTLQDNAAGTSLMPNYEPDLSNNDKVPGNTLSFESELPTVYSLSDECPEQESESVSGIPTKSASADKINDSSDNMEDTFCKESSQSEDYLISPMSEDSADTDDVFVSPNKPRTTEDLFAAIHRSKRKVLGRKEAGDNSVKNKSKPITGSSGASASSQKSPGLIYRSAKKSNTSNEEFKLLLLKKGSRSDTSYRMSATEILKSPVTPKSLGDLALDSSSSSEEGGQLSPGSDAFSPLSPCSPRVISEGFSPKSGTMSAASRVGRSRVPPAASSSRYSVRCRFYNAPMQAISEGETENSDGSPHDDRSSQSSM

Target ORF information:

RefSeq Version	XM_002934358.4
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis NHS actin remodeling regulator (nhs), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002934358.4

ORF Insert Sequence:

ATGCCCTTTG CCAAGAGGAT TGTTGAGCCC CAGTGGCTGT GCAGGCATGC CATCACCCTT 
CAAGAGGGTA GCTGCTGCCG GGATCAGGGA GAAGAGCACA GGCAGCAGCA CCCCCGGGGC 
AACTCCAGGG AAGAATTGTC AGGGGTGGTG GCACCTTCAG TGCAGGTGCC CTCTGATGAA 
AGGGAAACAG TGCCCGGTGA TGGAAAAGAT AAAGATGTGG CGCCACTGGA TTTGGGCGCA 
TTCAACAACG TCGCCTTGTC ACGGATTTTG CGTCAGTTGT CGGACATGGC CCGGCATGCC 
TGCTCCCTTT TCCATGAGAT TGAGGTGGAT ATACAGGTGA TCGGTCGGCG GGTGAGAACA 
TTGCAAGGGA AAATCAGCAA TGTCCAGCAG GTTGTGACCA GTCTGGATCC AAAACAGGAG 
ACAGTCCCGG TCTCAAACCT GGATGAAGAA AGCAAACTGG AAGTGTACTA CAAGGCCAAC 
TGGCACCAGC AGAGGAATAT TTTCCTGCCA AACACGAGGC CCCCGTGTGT GGAAGAGTTG 
CACCGCCATG CCAAGCAGAG TCTGCGTTCA ATACGGAGAG AGCAACGGAT CCGGGCAGAT 
AATAGAGAAA GAAGGTTTCA GGGCTCGATA TTTACAGCCC CACCGCTTCC CACATACCCA 
GCAGTTCACA GTCTGAAGAG ACGAGAAGTC AAAGAGCACC ATATTGTACA ATTTAACAGA 
ACCCGTTCGC ACTCCCCCAC TGAATGTTGC CATTTGACCC CATGGAGTAG AAAGGCTCAT 
CCACCTCAGG AGGAAGATGA TATCACTTTA GGACAAAGGC CGAAAAACCC TATTCCCAAT 
GTTCCATCTA CACTGGATAA GCAAACCAAC TGGAACAAGG CTTTACCTCT CCCCACTCCT 
GAGGAGAAGA TGAAACAGGA GTCCCAAGTG ATTACTTCTT GCATTATACC CATCAATGTT 
ACTGGGGTTG GATTTGATAG AGAGGCGAGC ATACGCTGTT CACTTGTTCA TTCACAATCT 
GTACTGCAGC GTAGACGGAA ACTGAGAAGA AGGAAAACTA TATCAGGAAT TCCCAGAAGA 
GTTCAACAAG AAATAGATTC TGATGAATCC CCAGTTGCAA GAGAGCGTAA TGTTATAGTT 
CACACCAACC CTGAACTTGC CAGCTCTGCA AACAGGAGAT CTGGTACACG TGATACAGAG 
TGCCAAACAG ATGATATACT CATTACTGCT CCATCTCGTA GAAGAATTAG AGCTCAAAGG 
GGCCAGGGAG TTGCAGCATC TCTCTCTCAT TCTGCTGGTA ACATTGCAGC CCTAACAGAG 
AGTGGTGACC AGATGTTTAA AACCACAGTA TCCAGTCGCA TTCGATCAAG GAGTCTTCCT 
AGAGAAGGTG TTAGAGCAAG CAATGATCAT GATTGTTGCA CACAGTCATC AACATATGAA 
GAAGACTGCT ATATGCCCTG CACTGAGAGG ACACTAAAGA AGGATAAAGA CAGATTAACT 
AGTCAGGAGT CTCCAGACCA CCAGCCATTA GGCTTATCCT ATTCCCAACA CCGACACAGC 
CCGCCGTTAA ATTTGAATGA AAGGGGGAGA TCAAGGCTAT CAAGAATGGC AGATTCAGGG 
AGCTGTGATA TCTCTTCAAA TTCAGATACA TTTGGAAGCC CTGTTCACTC AATTTCAGCA 
GCAGGTGTGT TGTTGAGCAC ACACATTGAT CAAAAAGATG ACCATCAGTC GTCTAGTGGA 
AACTGGAGCG GAAGTAGCTC AACCTGTCCC TCCCAGACAT CAGAAACAAT CCCTCCAGCT 
GCATCTCCTC CACTAACTGG TTCTTCCCAC TGTGATTCTG AATTATCCTT GAACACAGCT 
CCCCACGCTG GTGAAGATTC TAATGTTTTT ATGATTGAAC AATACAGTGA TGACCACATA 
GATAGCATAA GAGGTCACAG AGCCAGTTCC TTTACATCTA CAGTTGCTGA CCTGCTTGAC 
GATCCAAACA ACAGTAATAC TAGTGACAGC GAATGGAATT ACCTTCACCA TCACCATGAT 
GCATCATGTC ATCAAGATTT TAGTCCACCG CACTTAAAGA CAGATCGCCT AGCATGCCCT 
AGTTTTACCA GTATGGGCAC TTATGACAGT TTTCTTGAAA AACCTGCATC TGAAAAAGCA 
GATTCAGTTT CATATTATTC TGTAGACACT GAAGGATATT ATACCTCCAT GCATTTTGAC 
TGTGGTCTGA ATACTAGTAG GAGTTATATT TGTAACTATG CACCAACAGG GTCAGAAAGT 
GATCAAGGTC CTGGTGTTTC TTCTGTGAAT ACCGATAATC CTTGGCAGTG TTATATGGAT 
CAAGGCAATG AAGGAAACCA GAACATCTCA TTTAAGAAAC CAAAGGCAAA GCCTACACCA 
CCAAAACGTT GTTCATCTTT AAGGAAATCT GACAACAACA CAGATATTCC TGAGAAGAAA 
GAACCAAAGA TAGTATGTGG TCAGCAAGTG AATCACTCTT CCAGGGAAAT GAAGCTGCCA 
CTTGAGATTT CTAATGTTCC TTTAAGAGTG GAAAACTTTG CTACACAACA TCAACAGGAA 
ATACCATGGG CTAACCAGGA TGACACTGAG TTAAAGAACA CTCAATTTTT AACCACAGAT 
ATTACTTCTT TTAAAGATGA GAGTGGAGAG CAGTCTCACT ATGCAGATCT GTGGCTCCTC 
AATGACTTGA AAACAAGTGA TCCTTATAGG TCATTATCAA ACTCAAGCAC TGCCACAGGT 
ACTACAGTTA TAGAATGCAT TAAGTCACCA GAAAGTTCAG AGTCACAGAC ATCACAGTCT 
GAATCCAGAG CAACGTCGCC ATCTCTTCCT TCTGTGGAAA ATGAGTTCAA GTTGTCTTCT 
CCAGAAAAGC TTGCTGGCTT AGCATCACCA TCAAGTGGTT ACTCTAGCCA GTCAGAAACC 
CCTACATCCT CCTTTCCCAT ACCTTTCTTT TCTGGGCCCC TGTCACCTGG TGGTAGCAAA 
CGAAAGCCAA AAGTTCCAGA AAGAAAATCG TCCTTGCAAC ATCCTTCTGC AAAATGTGGA 
AGAGAACTGG AGCTTCCAAC TATACCTCCG ACCCATCTTG ACCTAAGTGC TCTTCATGTT 
TTACATAAAC CATTACCTTA CAGACCACAG ATGCATGCTT TCAGTCATAA TAATCAGAAT 
ATGTCAGAAG ATCAGATTGA CTCAAATTCT TCACCAGCTC TTGCCATAAC ACCATCTGTA 
CTTAAATCTG TCCATCTCAG ATCTGTCAAC AAGCAACATA AAAAGTCAAA GCAAAGAGAA 
ACCTGTAATC TTGTGTGCTT GCAGGATCCT ACAATAACAG TGGATACCTA TACCCCAGAA 
AAGGCAATTG TGACATCAGC CAGAAAATCA ATTTTGCGTC AGTTTTCTAC AGAGGAAACC 
ATCCTACCAT ATAAGGATTC CTCACCAGTT GACACCAGTC CAGAAAGTAT GTTTTTTGAC 
AAGTGTGCAC TATTCACTGG TCATGGAATA TACAGAGAAG AGAAAGGTAC AAATATATTT 
GGAGTCACTA AAGAACCTTG GACTAATGAA ATTGTGCAAG AAACTGTGTT TTTAGTACAG 
CCAGAGACCA CAACAACAAC TTTTCAAGAG TACTACGAGA AAAAGAACTG TGGCCATGCT 
CAAGATGATC AACTTAAGAT GACACCAGAC TTACATATAC AGGATGATGT AATGACTATA 
CCAGAGCCTG TAACAGGAAC AACTACTGGG TACACATTGC AAGACAATGC TGCTGGGACA 
TCTTTAATGC CTAACTATGA GCCAGACTTG AGCAACAATG ATAAAGTGCC TGGAAACACT 
CTCAGCTTTG AATCAGAGCT CCCCACTGTA TATTCACTCA GCGATGAATG TCCAGAGCAG 
GAATCTGAAT CTGTATCAGG AATTCCAACC AAAAGTGCCT CAGCGGACAA AATTAATGAT 
TCATCTGACA ACATGGAAGA CACCTTCTGC AAAGAATCAT CTCAGAGTGA GGACTACCTA 
ATTTCACCAA TGAGTGAGGA CTCGGCAGAC ACAGATGATG TGTTTGTGTC CCCTAACAAA 
CCTCGTACAA CAGAGGATCT TTTTGCAGCC ATTCACAGAT CCAAACGAAA AGTACTTGGA 
AGAAAAGAAG CTGGGGACAA CTCTGTGAAA AACAAATCAA AACCAATAAC TGGGAGCAGT 
GGGGCCTCTG CTAGCTCTCA AAAGTCTCCT GGTCTCATCT ATAGGAGTGC TAAAAAATCC 
AATACATCCA ATGAAGAATT TAAGTTGCTG CTTTTGAAAA AGGGCAGCAG GTCAGATACC 
AGCTACCGCA TGTCTGCTAC TGAGATTCTG AAGAGTCCAG TAACACCTAA ATCCCTTGGT 
GATCTAGCAC TTGACTCCTC ATCAAGCTCT GAAGAGGGTG GTCAGTTATC ACCTGGAAGT 
GATGCATTTT CGCCTTTATC CCCTTGTTCT CCTCGTGTTA TATCTGAGGG GTTTTCTCCA 
AAAAGTGGAA CTATGTCTGC TGCCTCAAGA GTGGGAAGGT CCAGAGTGCC TCCTGCTGCA 
AGCAGCAGCC GTTACAGTGT CAGGTGCCGT TTTTACAATG CTCCAATGCA GGCCATATCA 
GAAGGAGAAA CAGAAAACTC GGATGGTAGC CCTCATGATG ACAGGTCCTC TCAGAGCTCA 
ATGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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nhs cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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