nhsb gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021479859.1	XP_021335534.1	Nance-Horan syndrome protein isoform X6
XM_009306119.2	XP_009304394.1	Nance-Horan syndrome protein isoform X2
XM_009306119.3	XP_009304394.1	Nance-Horan syndrome protein isoform X3
XM_021479860.1	XP_021335535.1	Nance-Horan syndrome protein isoform X7
XM_009306118.3	XP_009304393.1	Nance-Horan syndrome protein isoform X2
XM_009306118.2	XP_009304393.1	Nance-Horan syndrome protein isoform X1
XM_009306120.2	XP_009304395.1	Nance-Horan syndrome protein isoform X3
XM_021479857.1	XP_021335532.1	Nance-Horan syndrome protein isoform X1
XM_021479858.1	XP_021335533.1	Nance-Horan syndrome protein isoform X4
XM_009306120.3	XP_009304395.1	Nance-Horan syndrome protein isoform X5

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa148024	XM_021479857.1 Latest version!	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa148025	XM_021479858.1 Latest version!	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa148026	XM_021479859.1 Latest version!	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa148027	XM_021479860.1 Latest version!	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa43148	XM_009306118.3 Latest version!	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa43149	XM_009306119.3 Latest version!	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa43150	XM_009306120.3 Latest version!	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
ODa43149	XM_009306119.2	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa43148	XM_009306118.2	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
ODa43150	XM_009306120.2	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
Hide Old Accession Versions >>

nhsb ( XM_021479857.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335532.1 Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa148024
Clone ID Related Accession (Same CDS sequence)	XM_021479857.1
Accession Version	XM_021479857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Nance-Horan syndrome protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA 
GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT 
CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA 
CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC 
AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC 
CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG 
AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT 
GAATTCAAAC CTGTCCTCAG AAAGTATCAG CGCTCCCGCT CCCCCTCCCC TGTTCAGTGT 
TGTTACTTCA TTCCCTGGAT TAGAAAGGCT GGAACCAACA CAGAAACGGA TGGAGAACTA 
CAAGTGATGA GCCATAGGCC TAAATGTCCT GTTCCCAATG CACCTACCAC CCTGGACAAA 
CAGACTAACT GGTCTAAAGC CCTGCCTCTT CCCACTCCAG AAGAAAGAAT AAAGAATGAC 
TCTCAAGTGA TTTCATCCTG TATCATTCCA ATAAATGTTT CGGGTGTTGG ATTTGACAGA 
GAAGCCAGTG CTCGCTGCTC TCTTGTTCAC TCGCAATCGG TTCTACAGAG ACGGCGGAAA 
CTCAGGAGGC GAAAAACGAT CACTGGGATC CCCAAACGAG TTCAACAAGA CATGGATTCT 
GACGAATCCC CTGTTGCTAG AGAAAGAACA GTGATAATCC ACGCCAATCC ACACAAGTCC 
CACGAGTGGC ATGAGGAGCT CTCCTTGAGT GGCAGAGTAT TGCATACTAA GGATTCGGGC 
TGTCAGACAG ATGACTTCTT GATTACTGCT CCATCCCGAA GACGCATCCG TGCCCAAAGA 
GGTCAGGGAA TTCCAGCGTC TCTCTCTCAC TCCACTGGAA ACATTACCTC CCTCCCGGAT 
CGTTCTGATA CGGTTTATGC TGCAGCATCA GCCACTCGTG TCCGTTCTAG GAGCCTTCCA 
CGTGAGGGTG GTCGGCTTCT GGATGAGGAC CAAGATGACA GTGATGATGA TGATGATGAA 
GATGATGATG AAGATGATGA AGAGGAAGAT GAGGAGCTCT CTCCATATGA AGCCGAAGAC 
TTCCTGCCAG GTCCCGGACA GAGAATTCCA AAGGATGAGG AAGAGAGTAC TGACGACCAA 
GCCATGCCAG AGCTACAGTT TGGAAGTCTT AAGCGTATGC AGCATTCAGA GAGCCCTGAC 
CACACATGGA TTGAGAGAGG CAGATCCAGA CTCCCACGGA AAGTAGACAT GGGGAGCTGT 
GAGATTTCCT CTAGTTCAGA TACTTTCAGC AGCCCCATAC ACTCTGCATC CACTACAGGA 
GTGCTTGGCA GCCAGATTGA CCATAAAGAG GATCACCAGT CCTCAAGTGG CAACTGGAGT 
GGAAGCAGCT CCACTTGCCC ATCGCAGACA TCCGAGACTC TTCCACCTGC TGCCTCCCCT 
CCCTTGACGG GATCCTCACA CTGTGATTCA GAACTTTCCC TGAATACTGG GTCCCATGTC 
ATAGATGACA ACACTGGTTT CATAATTGAT CCCTATCACA TAGACAAGCC ACAGGGACAG 
GGGCAACGAT CCAATTCATT TACCTCATCA GCTACTGATC AGATGGATGA TGCAGGGGTC 
AGCACTGCTA GTGATGGGGA GTGGAACTGT GCCCAGGACC AGCAGGATCA GTCCTGCACC 
CAAGACTTCA GCCCAAAGCG TTCCAGAGAG GATGAGAGTG GTGTCAGCTG CCCTAGTTAT 
TCTAGTGGGG AAACTTACGA GAGCTTCAGT GACCAAGCAT CCGCTAGAAA ATCTGAGATG 
CACTACATTG TCGACACTGA AGGATTCTAT TCCTCCATGC ACTTTGACTC TGGTCTTAAG 
GGTAGCAGAA GCTACTACAA CTATGCAGCC ATTGACCCCG ACTGTGGCCC AAGTGGTAAT 
ATAGCACCTG GCATGACTGA ATACCCTCAG CCAGAGTACA GAGCCACCAG GACACTGGGC 
AGACCCTGTC TCTCCTTAAG AAAACCAAAG GCCAAGCCAC CCCCACCAAA ACGTAGCTCT 
TCATTAAAGG AAACAAGCAG TGATGTGAAC GTTCCAGAGC AGAACGAACC AAAGATTACT 
TGTGAGTTGC CACTGCCCTT GTCTTCCAGG GAGATGAAAC TGCAGCTGGA CTTGGCTGAT 
TCTGCAGGGC ACCTTGAGAC TGCAGGTCTT GAATCACTTG GTGCATGGGG AGTGGAAAAT 
GTTAGGGACA TGCTTGACTG CTCCTCCCCA TTCAGTTCCT CAGACACACA TTCCTTCAAA 
GATGAAGGTG CTGTGCAAGC AGACTATGCA GACCTCTGGC TTCACAATGA CTTGAAATCA 
AATGATCCTT ATCGGTCCCT CTCTAACTCT AGCTCTGCTA CAGGTACAAC TGTTATTGAA 
TGCATCAAGT CACCAGAAAG ATCAGAAACG CACACCTGTC AACCCAGGTC CAGACCTTCT 
TCCCCAACTC TTCCTCCACC TGAAAGTGAA TTTAAGCTTG CTTCTCCCGA AAAGCTGGCA 
GGCTTGGCGT CCCCTTCCAG TGGATATTCC AGTCAGTCTG AAACACCTAC ATCTTCCTTC 
CCCTCTGCTT TCTTCCCAGG GCCCCTGTCT CCAACCAGTG GGAAGAGGAA GCCCAAAGTC 
CCTGAAAGGA AGTCCTCACT TTGTTCCTTA CAGCAGCAAC AGCTTTCAGT CAGAGACCCA 
GGCATTTCCT GTAGGAGAGA AACTGACTTC TATGCCATAC CCCCAAGTCA CCTTGACCTA 
AGTGCTCTTC ACAGTAAGCA CTTTCCTCAC AGAAATCAAA TGCACATCCT CCACCAGAAT 
AAGCAGAAAG CTGCCATAGC AGCAGCTGCT GCAGCAGAAG CTCGCAGTGC AGCAGCTGCT 
GCAGCAGAAG CTCGCAGTGC AGCAGCTGCT GCAGCAGAAG CTCGCAGTGC AGCAACTGCA 
GCCGCTGCTG CCGAGGCCTG CACTGCAGCT ACAGCTTCTA CTAAAGAGAG TGCAACAAAT 
ACAGCACCCA TCTCAGCCCA TTTGGCTATT ACTCCAACGG TTCTTAGATC AGTGCAACTG 
CGATCTATAT GTAGACCATC TGATGGCAAC CAAGGGCTTG ATCAAGACAG TTCAAATCTC 
ATAACTCGTC CTAAGTGTCC CACAATAATA ACTGATGCCC CATCATCTAG CAACAGGCAC 
AACAGGAAGC CACCAGCCTA CAAACCCCCT GCTGCACAGG TTTGTGATTC ACAGATTCCA 
CTGGTGGAAA ACATTGTTTT TCCACAAGAA GAAGTGAGAG TGAGACAGGA GAGGTTTGGT 
CCTGGTACTT ACTGGGCAAT GACTGCTTTC AGAACTCCTG TGGACCCTAA TTGTGAAAAA 
GAAGTCTCTT TAAAAAGGTC ATCTCAGTGT TTTCAGCAAG AACAAGACAG CAGAAGGTGC 
CTAGATGTGC AAAAGACATT GTCACCAGAG AGACTAAAAC AAGATTTGAA TCAACTATCG 
CGGCCAGACC CTTCAACACA GTCCATGTGT TTGGCCAGCA CAATGCCACC CCCTGCTTAT 
AGTGAGATAC AGAGGAGCAA TTTTGTACTG TGCAACAGTC CTGACAAAGT GCCTGTTTCA 
ACTCAAGAGG CATCGCATTC TTACACAATC AGCGATGTAC AAGGCAGAGA GGAGGATTAT 
GAGACATCAG GTGTCGCAAC CAGAAGTGCC TCACAGGACA TAAGGGAAGA AGAGTCAACC 
CCAGATACAG AGGACTATTT CAGTAAAGAC TCTACTCCCA GTGATAATTC ATCCTCTCCT 
TTGACCGATG ACTCCAGACT TGATGATGAT GTTTTCCCAT CTCCCAATAA ACTCCGCACA 
ACTGAAGATC TTTTTGCTAT GATACACAGA TCTAAAAGGA AAGTGCTGGG ACGCAAAGAT 
TCAGGGGAAG TCAGTGGAAA AGGTCGGCCT GTTGTACCAC CTGTGACCAC CCCTGTAAGC 
AATCCTACTG TATGCCCGGT CATCCCTGCT CCCCCAGTTC CTTCAAACAA CTCCCAGCGA 
GCCTCAGGAC CCATCTACAG GAGTGCCAAA AAGTCTAGTA CATCCAGTGA GGAGTTCAAA 
CTCCTTCTGC TTAAGAAGGG TAGTCGCACA GACTCAAGTT ATCGCATGTC TGCTACAGAG 
ATCCTTAAGA GCCCCATCAC ACCCAAGACT CAAGCAGAGC TGCTGTTAGA GGCCATGAGG 
CAACCAGAGG AGATCCCCTT ACCCCAACTG GATTCCACCA GCAGTGGAGA TCCACTTCCA 
AGTCCATTCC CTAAGGCCAA CAGTGAGGGA TTCTCCCCAA AAACACTCAC CATGTCAGCT 
GCCTCCAGAC AAGGACGTTC TAGAATTCCA CCTGCAGCGA ACAGCAGTCG CTATAGCACG 
CGGAGTCGGC TGTACACTGC CCCAATGCAG GCCATATCAG AAGGAGAGAC TGAGAACTCG 
GATGGAAGTC CACACGATGA CCGCTCCTCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335532.1
CDS	321..5213
Translation	MPFAKRIVEPQLLVRHPIPNDEGLLFEDLCSITNVALSRTLRQLSDLSKHACSLFQELENEIVSTNQRVWALQNKIGKIQQTASALDPKMEAVRFRSISIAVSNLDIESKLTSHYQAPWHQQHNLFHTCTRPACLEELHRHAKLNLRALHRDQQQRQRSTSRERSRVTISISVAPPMPTFPSTHKLRRREQRGRHSRMERSVRESDVQTIQRKERPAKDSEFKPVLRKYQRSRSPSPVQCCYFIPWIRKAGTNTETDGELQVMSHRPKCPVPNAPTTLDKQTNWSKALPLPTPEERIKNDSQVISSCIIPINVSGVGFDREASARCSLVHSQSVLQRRRKLRRRKTITGIPKRVQQDMDSDESPVARERTVIIHANPHKSHEWHEELSLSGRVLHTKDSGCQTDDFLITAPSRRRIRAQRGQGIPASLSHSTGNITSLPDRSDTVYAAASATRVRSRSLPREGGRLLDEDQDDSDDDDDEDDDEDDEEEDEELSPYEAEDFLPGPGQRIPKDEEESTDDQAMPELQFGSLKRMQHSESPDHTWIERGRSRLPRKVDMGSCEISSSSDTFSSPIHSASTTGVLGSQIDHKEDHQSSSGNWSGSSSTCPSQTSETLPPAASPPLTGSSHCDSELSLNTGSHVIDDNTGFIIDPYHIDKPQGQGQRSNSFTSSATDQMDDAGVSTASDGEWNCAQDQQDQSCTQDFSPKRSREDESGVSCPSYSSGETYESFSDQASARKSEMHYIVDTEGFYSSMHFDSGLKGSRSYYNYAAIDPDCGPSGNIAPGMTEYPQPEYRATRTLGRPCLSLRKPKAKPPPPKRSSSLKETSSDVNVPEQNEPKITCELPLPLSSREMKLQLDLADSAGHLETAGLESLGAWGVENVRDMLDCSSPFSSSDTHSFKDEGAVQADYADLWLHNDLKSNDPYRSLSNSSSATGTTVIECIKSPERSETHTCQPRSRPSSPTLPPPESEFKLASPEKLAGLASPSSGYSSQSETPTSSFPSAFFPGPLSPTSGKRKPKVPERKSSLCSLQQQQLSVRDPGISCRRETDFYAIPPSHLDLSALHSKHFPHRNQMHILHQNKQKAAIAAAAAAEARSAAAAAAEARSAAAAAAEARSAATAAAAAEACTAATASTKESATNTAPISAHLAITPTVLRSVQLRSICRPSDGNQGLDQDSSNLITRPKCPTIITDAPSSSNRHNRKPPAYKPPAAQVCDSQIPLVENIVFPQEEVRVRQERFGPGTYWAMTAFRTPVDPNCEKEVSLKRSSQCFQQEQDSRRCLDVQKTLSPERLKQDLNQLSRPDPSTQSMCLASTMPPPAYSEIQRSNFVLCNSPDKVPVSTQEASHSYTISDVQGREEDYETSGVATRSASQDIREEESTPDTEDYFSKDSTPSDNSSSPLTDDSRLDDDVFPSPNKLRTTEDLFAMIHRSKRKVLGRKDSGEVSGKGRPVVPPVTTPVSNPTVCPVIPAPPVPSNNSQRASGPIYRSAKKSSTSSEEFKLLLLKKGSRTDSSYRMSATEILKSPITPKTQAELLLEAMRQPEEIPLPQLDSTSSGDPLPSPFPKANSEGFSPKTLTMSAASRQGRSRIPPAANSSRYSTRSRLYTAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_021479857.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479857.1

ORF Insert Sequence:

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA 
GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT 
CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA 
CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC 
AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC 
CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG 
AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT 
GAATTCAAAC CTGTCCTCAG AAAGTATCAG CGCTCCCGCT CCCCCTCCCC TGTTCAGTGT 
TGTTACTTCA TTCCCTGGAT TAGAAAGGCT GGAACCAACA CAGAAACGGA TGGAGAACTA 
CAAGTGATGA GCCATAGGCC TAAATGTCCT GTTCCCAATG CACCTACCAC CCTGGACAAA 
CAGACTAACT GGTCTAAAGC CCTGCCTCTT CCCACTCCAG AAGAAAGAAT AAAGAATGAC 
TCTCAAGTGA TTTCATCCTG TATCATTCCA ATAAATGTTT CGGGTGTTGG ATTTGACAGA 
GAAGCCAGTG CTCGCTGCTC TCTTGTTCAC TCGCAATCGG TTCTACAGAG ACGGCGGAAA 
CTCAGGAGGC GAAAAACGAT CACTGGGATC CCCAAACGAG TTCAACAAGA CATGGATTCT 
GACGAATCCC CTGTTGCTAG AGAAAGAACA GTGATAATCC ACGCCAATCC ACACAAGTCC 
CACGAGTGGC ATGAGGAGCT CTCCTTGAGT GGCAGAGTAT TGCATACTAA GGATTCGGGC 
TGTCAGACAG ATGACTTCTT GATTACTGCT CCATCCCGAA GACGCATCCG TGCCCAAAGA 
GGTCAGGGAA TTCCAGCGTC TCTCTCTCAC TCCACTGGAA ACATTACCTC CCTCCCGGAT 
CGTTCTGATA CGGTTTATGC TGCAGCATCA GCCACTCGTG TCCGTTCTAG GAGCCTTCCA 
CGTGAGGGTG GTCGGCTTCT GGATGAGGAC CAAGATGACA GTGATGATGA TGATGATGAA 
GATGATGATG AAGATGATGA AGAGGAAGAT GAGGAGCTCT CTCCATATGA AGCCGAAGAC 
TTCCTGCCAG GTCCCGGACA GAGAATTCCA AAGGATGAGG AAGAGAGTAC TGACGACCAA 
GCCATGCCAG AGCTACAGTT TGGAAGTCTT AAGCGTATGC AGCATTCAGA GAGCCCTGAC 
CACACATGGA TTGAGAGAGG CAGATCCAGA CTCCCACGGA AAGTAGACAT GGGGAGCTGT 
GAGATTTCCT CTAGTTCAGA TACTTTCAGC AGCCCCATAC ACTCTGCATC CACTACAGGA 
GTGCTTGGCA GCCAGATTGA CCATAAAGAG GATCACCAGT CCTCAAGTGG CAACTGGAGT 
GGAAGCAGCT CCACTTGCCC ATCGCAGACA TCCGAGACTC TTCCACCTGC TGCCTCCCCT 
CCCTTGACGG GATCCTCACA CTGTGATTCA GAACTTTCCC TGAATACTGG GTCCCATGTC 
ATAGATGACA ACACTGGTTT CATAATTGAT CCCTATCACA TAGACAAGCC ACAGGGACAG 
GGGCAACGAT CCAATTCATT TACCTCATCA GCTACTGATC AGATGGATGA TGCAGGGGTC 
AGCACTGCTA GTGATGGGGA GTGGAACTGT GCCCAGGACC AGCAGGATCA GTCCTGCACC 
CAAGACTTCA GCCCAAAGCG TTCCAGAGAG GATGAGAGTG GTGTCAGCTG CCCTAGTTAT 
TCTAGTGGGG AAACTTACGA GAGCTTCAGT GACCAAGCAT CCGCTAGAAA ATCTGAGATG 
CACTACATTG TCGACACTGA AGGATTCTAT TCCTCCATGC ACTTTGACTC TGGTCTTAAG 
GGTAGCAGAA GCTACTACAA CTATGCAGCC ATTGACCCCG ACTGTGGCCC AAGTGGTAAT 
ATAGCACCTG GCATGACTGA ATACCCTCAG CCAGAGTACA GAGCCACCAG GACACTGGGC 
AGACCCTGTC TCTCCTTAAG AAAACCAAAG GCCAAGCCAC CCCCACCAAA ACGTAGCTCT 
TCATTAAAGG AAACAAGCAG TGATGTGAAC GTTCCAGAGC AGAACGAACC AAAGATTACT 
TGTGAGTTGC CACTGCCCTT GTCTTCCAGG GAGATGAAAC TGCAGCTGGA CTTGGCTGAT 
TCTGCAGGGC ACCTTGAGAC TGCAGGTCTT GAATCACTTG GTGCATGGGG AGTGGAAAAT 
GTTAGGGACA TGCTTGACTG CTCCTCCCCA TTCAGTTCCT CAGACACACA TTCCTTCAAA 
GATGAAGGTG CTGTGCAAGC AGACTATGCA GACCTCTGGC TTCACAATGA CTTGAAATCA 
AATGATCCTT ATCGGTCCCT CTCTAACTCT AGCTCTGCTA CAGGTACAAC TGTTATTGAA 
TGCATCAAGT CACCAGAAAG ATCAGAAACG CACACCTGTC AACCCAGGTC CAGACCTTCT 
TCCCCAACTC TTCCTCCACC TGAAAGTGAA TTTAAGCTTG CTTCTCCCGA AAAGCTGGCA 
GGCTTGGCGT CCCCTTCCAG TGGATATTCC AGTCAGTCTG AAACACCTAC ATCTTCCTTC 
CCCTCTGCTT TCTTCCCAGG GCCCCTGTCT CCAACCAGTG GGAAGAGGAA GCCCAAAGTC 
CCTGAAAGGA AGTCCTCACT TTGTTCCTTA CAGCAGCAAC AGCTTTCAGT CAGAGACCCA 
GGCATTTCCT GTAGGAGAGA AACTGACTTC TATGCCATAC CCCCAAGTCA CCTTGACCTA 
AGTGCTCTTC ACAGTAAGCA CTTTCCTCAC AGAAATCAAA TGCACATCCT CCACCAGAAT 
AAGCAGAAAG CTGCCATAGC AGCAGCTGCT GCAGCAGAAG CTCGCAGTGC AGCAGCTGCT 
GCAGCAGAAG CTCGCAGTGC AGCAGCTGCT GCAGCAGAAG CTCGCAGTGC AGCAACTGCA 
GCCGCTGCTG CCGAGGCCTG CACTGCAGCT ACAGCTTCTA CTAAAGAGAG TGCAACAAAT 
ACAGCACCCA TCTCAGCCCA TTTGGCTATT ACTCCAACGG TTCTTAGATC AGTGCAACTG 
CGATCTATAT GTAGACCATC TGATGGCAAC CAAGGGCTTG ATCAAGACAG TTCAAATCTC 
ATAACTCGTC CTAAGTGTCC CACAATAATA ACTGATGCCC CATCATCTAG CAACAGGCAC 
AACAGGAAGC CACCAGCCTA CAAACCCCCT GCTGCACAGG TTTGTGATTC ACAGATTCCA 
CTGGTGGAAA ACATTGTTTT TCCACAAGAA GAAGTGAGAG TGAGACAGGA GAGGTTTGGT 
CCTGGTACTT ACTGGGCAAT GACTGCTTTC AGAACTCCTG TGGACCCTAA TTGTGAAAAA 
GAAGTCTCTT TAAAAAGGTC ATCTCAGTGT TTTCAGCAAG AACAAGACAG CAGAAGGTGC 
CTAGATGTGC AAAAGACATT GTCACCAGAG AGACTAAAAC AAGATTTGAA TCAACTATCG 
CGGCCAGACC CTTCAACACA GTCCATGTGT TTGGCCAGCA CAATGCCACC CCCTGCTTAT 
AGTGAGATAC AGAGGAGCAA TTTTGTACTG TGCAACAGTC CTGACAAAGT GCCTGTTTCA 
ACTCAAGAGG CATCGCATTC TTACACAATC AGCGATGTAC AAGGCAGAGA GGAGGATTAT 
GAGACATCAG GTGTCGCAAC CAGAAGTGCC TCACAGGACA TAAGGGAAGA AGAGTCAACC 
CCAGATACAG AGGACTATTT CAGTAAAGAC TCTACTCCCA GTGATAATTC ATCCTCTCCT 
TTGACCGATG ACTCCAGACT TGATGATGAT GTTTTCCCAT CTCCCAATAA ACTCCGCACA 
ACTGAAGATC TTTTTGCTAT GATACACAGA TCTAAAAGGA AAGTGCTGGG ACGCAAAGAT 
TCAGGGGAAG TCAGTGGAAA AGGTCGGCCT GTTGTACCAC CTGTGACCAC CCCTGTAAGC 
AATCCTACTG TATGCCCGGT CATCCCTGCT CCCCCAGTTC CTTCAAACAA CTCCCAGCGA 
GCCTCAGGAC CCATCTACAG GAGTGCCAAA AAGTCTAGTA CATCCAGTGA GGAGTTCAAA 
CTCCTTCTGC TTAAGAAGGG TAGTCGCACA GACTCAAGTT ATCGCATGTC TGCTACAGAG 
ATCCTTAAGA GCCCCATCAC ACCCAAGACT CAAGCAGAGC TGCTGTTAGA GGCCATGAGG 
CAACCAGAGG AGATCCCCTT ACCCCAACTG GATTCCACCA GCAGTGGAGA TCCACTTCCA 
AGTCCATTCC CTAAGGCCAA CAGTGAGGGA TTCTCCCCAA AAACACTCAC CATGTCAGCT 
GCCTCCAGAC AAGGACGTTC TAGAATTCCA CCTGCAGCGA ACAGCAGTCG CTATAGCACG 
CGGAGTCGGC TGTACACTGC CCCAATGCAG GCCATATCAG AAGGAGAGAC TGAGAACTCG 
GATGGAAGTC CACACGATGA CCGCTCCTCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsb ( XM_021479858.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335533.1 Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa148025
Clone ID Related Accession (Same CDS sequence)	XM_021479858.1
Accession Version	XM_021479858.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Nance-Horan syndrome protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA 
GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT 
CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA 
CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC 
AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC 
CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGA 
CCAGCCAAGG ATTCTGAATT CAAACCTGTC CTCAGAAAGT ATCAGCGCTC CCGCTCCCCC 
TCCCCTGTTC AGTGTTGTTA CTTCATTCCC TGGATTAGAA AGGCTGGAAC CAACACAGAA 
ACGGATGGAG AACTACAAGT GATGAGCCAT AGGCCTAAAT GTCCTGTTCC CAATGCACCT 
ACCACCCTGG ACAAACAGAC TAACTGGTCT AAAGCCCTGC CTCTTCCCAC TCCAGAAGAA 
AGAATAAAGA ATGACTCTCA AGTGATTTCA TCCTGTATCA TTCCAATAAA TGTTTCGGGT 
GTTGGATTTG ACAGAGAAGC CAGTGCTCGC TGCTCTCTTG TTCACTCGCA ATCGGTTCTA 
CAGAGACGGC GGAAACTCAG GAGGCGAAAA ACGATCACTG GGATCCCCAA ACGAGTTCAA 
CAAGACATGG ATTCTGACGA ATCCCCTGTT GCTAGAGAAA GAACAGTGAT AATCCACGCC 
AATCCACACA AGTCCCACGA GTGGCATGAG GAGCTCTCCT TGAGTGGCAG AGTATTGCAT 
ACTAAGGATT CGGGCTGTCA GACAGATGAC TTCTTGATTA CTGCTCCATC CCGAAGACGC 
ATCCGTGCCC AAAGAGGTCA GGGAATTCCA GCGTCTCTCT CTCACTCCAC TGGAAACATT 
ACCTCCCTCC CGGATCGTTC TGATACGGTT TATGCTGCAG CATCAGCCAC TCGTGTCCGT 
TCTAGGAGCC TTCCACGTGA GGGTGGTCGG CTTCTGGATG AGGACCAAGA TGACAGTGAT 
GATGATGATG ATGAAGATGA TGATGAAGAT GATGAAGAGG AAGATGAGGA GCTCTCTCCA 
TATGAAGCCG AAGACTTCCT GCCAGGTCCC GGACAGAGAA TTCCAAAGGA TGAGGAAGAG 
AGTACTGACG ACCAAGCCAT GCCAGAGCTA CAGTTTGGAA GTCTTAAGCG TATGCAGCAT 
TCAGAGAGCC CTGACCACAC ATGGATTGAG AGAGGCAGAT CCAGACTCCC ACGGAAAGTA 
GACATGGGGA GCTGTGAGAT TTCCTCTAGT TCAGATACTT TCAGCAGCCC CATACACTCT 
GCATCCACTA CAGGAGTGCT TGGCAGCCAG ATTGACCATA AAGAGGATCA CCAGTCCTCA 
AGTGGCAACT GGAGTGGAAG CAGCTCCACT TGCCCATCGC AGACATCCGA GACTCTTCCA 
CCTGCTGCCT CCCCTCCCTT GACGGGATCC TCACACTGTG ATTCAGAACT TTCCCTGAAT 
ACTGGGTCCC ATGTCATAGA TGACAACACT GGTTTCATAA TTGATCCCTA TCACATAGAC 
AAGCCACAGG GACAGGGGCA ACGATCCAAT TCATTTACCT CATCAGCTAC TGATCAGATG 
GATGATGCAG GGGTCAGCAC TGCTAGTGAT GGGGAGTGGA ACTGTGCCCA GGACCAGCAG 
GATCAGTCCT GCACCCAAGA CTTCAGCCCA AAGCGTTCCA GAGAGGATGA GAGTGGTGTC 
AGCTGCCCTA GTTATTCTAG TGGGGAAACT TACGAGAGCT TCAGTGACCA AGCATCCGCT 
AGAAAATCTG AGATGCACTA CATTGTCGAC ACTGAAGGAT TCTATTCCTC CATGCACTTT 
GACTCTGGTC TTAAGGGTAG CAGAAGCTAC TACAACTATG CAGCCATTGA CCCCGACTGT 
GGCCCAAGTG GTAATATAGC ACCTGGCATG ACTGAATACC CTCAGCCAGA GTACAGAGCC 
ACCAGGACAC TGGGCAGACC CTGTCTCTCC TTAAGAAAAC CAAAGGCCAA GCCACCCCCA 
CCAAAACGTA GCTCTTCATT AAAGGAAACA AGCAGTGATG TGAACGTTCC AGAGCAGAAC 
GAACCAAAGA TTACTTGTGA GTTGCCACTG CCCTTGTCTT CCAGGGAGAT GAAACTGCAG 
CTGGACTTGG CTGATTCTGC AGGGCACCTT GAGACTGCAG GTCTTGAATC ACTTGGTGCA 
TGGGGAGTGG AAAATGTTAG GGACATGCTT GACTGCTCCT CCCCATTCAG TTCCTCAGAC 
ACACATTCCT TCAAAGATGA AGGTGCTGTG CAAGCAGACT ATGCAGACCT CTGGCTTCAC 
AATGACTTGA AATCAAATGA TCCTTATCGG TCCCTCTCTA ACTCTAGCTC TGCTACAGGT 
ACAACTGTTA TTGAATGCAT CAAGTCACCA GAAAGATCAG AAACGCACAC CTGTCAACCC 
AGGTCCAGAC CTTCTTCCCC AACTCTTCCT CCACCTGAAA GTGAATTTAA GCTTGCTTCT 
CCCGAAAAGC TGGCAGGCTT GGCGTCCCCT TCCAGTGGAT ATTCCAGTCA GTCTGAAACA 
CCTACATCTT CCTTCCCCTC TGCTTTCTTC CCAGGGCCCC TGTCTCCAAC CAGTGGGAAG 
AGGAAGCCCA AAGTCCCTGA AAGGAAGTCC TCACTTTGTT CCTTACAGCA GCAACAGCTT 
TCAGTCAGAG ACCCAGGCAT TTCCTGTAGG AGAGAAACTG ACTTCTATGC CATACCCCCA 
AGTCACCTTG ACCTAAGTGC TCTTCACAGT AAGCACTTTC CTCACAGAAA TCAAATGCAC 
ATCCTCCACC AGAATAAGCA GAAAGCTGCC ATAGCAGCAG CTGCTGCAGC AGAAGCTCGC 
AGTGCAGCAG CTGCTGCAGC AGAAGCTCGC AGTGCAGCAG CTGCTGCAGC AGAAGCTCGC 
AGTGCAGCAA CTGCAGCCGC TGCTGCCGAG GCCTGCACTG CAGCTACAGC TTCTACTAAA 
GAGAGTGCAA CAAATACAGC ACCCATCTCA GCCCATTTGG CTATTACTCC AACGGTTCTT 
AGATCAGTGC AACTGCGATC TATATGTAGA CCATCTGATG GCAACCAAGG GCTTGATCAA 
GACAGTTCAA ATCTCATAAC TCGTCCTAAG TGTCCCACAA TAATAACTGA TGCCCCATCA 
TCTAGCAACA GGCACAACAG GAAGCCACCA GCCTACAAAC CCCCTGCTGC ACAGGTTTGT 
GATTCACAGA TTCCACTGGT GGAAAACATT GTTTTTCCAC AAGAAGAAGT GAGAGTGAGA 
CAGGAGAGGT TTGGTCCTGG TACTTACTGG GCAATGACTG CTTTCAGAAC TCCTGTGGAC 
CCTAATTGTG AAAAAGAAGT CTCTTTAAAA AGGTCATCTC AGTGTTTTCA GCAAGAACAA 
GACAGCAGAA GGTGCCTAGA TGTGCAAAAG ACATTGTCAC CAGAGAGACT AAAACAAGAT 
TTGAATCAAC TATCGCGGCC AGACCCTTCA ACACAGTCCA TGTGTTTGGC CAGCACAATG 
CCACCCCCTG CTTATAGTGA GATACAGAGG AGCAATTTTG TACTGTGCAA CAGTCCTGAC 
AAAGTGCCTG TTTCAACTCA AGAGGCATCG CATTCTTACA CAATCAGCGA TGTACAAGGC 
AGAGAGGAGG ATTATGAGAC ATCAGGTGTC GCAACCAGAA GTGCCTCACA GGACATAAGG 
GAAGAAGAGT CAACCCCAGA TACAGAGGAC TATTTCAGTA AAGACTCTAC TCCCAGTGAT 
AATTCATCCT CTCCTTTGAC CGATGACTCC AGACTTGATG ATGATGTTTT CCCATCTCCC 
AATAAACTCC GCACAACTGA AGATCTTTTT GCTATGATAC ACAGATCTAA AAGGAAAGTG 
CTGGGACGCA AAGATTCAGG GGAAGTCAGT GGAAAAGGTC GGCCTGTTGT ACCACCTGTG 
ACCACCCCTG TAAGCAATCC TACTGTATGC CCGGTCATCC CTGCTCCCCC AGTTCCTTCA 
AACAACTCCC AGCGAGCCTC AGGACCCATC TACAGGAGTG CCAAAAAGTC TAGTACATCC 
AGTGAGGAGT TCAAACTCCT TCTGCTTAAG AAGGGTAGTC GCACAGACTC AAGTTATCGC 
ATGTCTGCTA CAGAGATCCT TAAGAGCCCC ATCACACCCA AGACTCAAGC AGAGCTGCTG 
TTAGAGGCCA TGAGGCAACC AGAGGAGATC CCCTTACCCC AACTGGATTC CACCAGCAGT 
GGAGATCCAC TTCCAAGTCC ATTCCCTAAG GCCAACAGTG AGGGATTCTC CCCAAAAACA 
CTCACCATGT CAGCTGCCTC CAGACAAGGA CGTTCTAGAA TTCCACCTGC AGCGAACAGC 
AGTCGCTATA GCACGCGGAG TCGGCTGTAC ACTGCCCCAA TGCAGGCCAT ATCAGAAGGA 
GAGACTGAGA ACTCGGATGG AAGTCCACAC GATGACCGCT CCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335533.1
CDS	321..5168
Translation	MPFAKRIVEPQLLVRHPIPNDEGLLFEDLCSITNVALSRTLRQLSDLSKHACSLFQELENEIVSTNQRVWALQNKIGKIQQTASALDPKMEAVRFRSISIAVSNLDIESKLTSHYQAPWHQQHNLFHTCTRPACLEELHRHAKLNLRALHRDQQQRQRSTSRERSRVTISISVAPPMPTFPSTHKLRRREQRGRHSRMERPAKDSEFKPVLRKYQRSRSPSPVQCCYFIPWIRKAGTNTETDGELQVMSHRPKCPVPNAPTTLDKQTNWSKALPLPTPEERIKNDSQVISSCIIPINVSGVGFDREASARCSLVHSQSVLQRRRKLRRRKTITGIPKRVQQDMDSDESPVARERTVIIHANPHKSHEWHEELSLSGRVLHTKDSGCQTDDFLITAPSRRRIRAQRGQGIPASLSHSTGNITSLPDRSDTVYAAASATRVRSRSLPREGGRLLDEDQDDSDDDDDEDDDEDDEEEDEELSPYEAEDFLPGPGQRIPKDEEESTDDQAMPELQFGSLKRMQHSESPDHTWIERGRSRLPRKVDMGSCEISSSSDTFSSPIHSASTTGVLGSQIDHKEDHQSSSGNWSGSSSTCPSQTSETLPPAASPPLTGSSHCDSELSLNTGSHVIDDNTGFIIDPYHIDKPQGQGQRSNSFTSSATDQMDDAGVSTASDGEWNCAQDQQDQSCTQDFSPKRSREDESGVSCPSYSSGETYESFSDQASARKSEMHYIVDTEGFYSSMHFDSGLKGSRSYYNYAAIDPDCGPSGNIAPGMTEYPQPEYRATRTLGRPCLSLRKPKAKPPPPKRSSSLKETSSDVNVPEQNEPKITCELPLPLSSREMKLQLDLADSAGHLETAGLESLGAWGVENVRDMLDCSSPFSSSDTHSFKDEGAVQADYADLWLHNDLKSNDPYRSLSNSSSATGTTVIECIKSPERSETHTCQPRSRPSSPTLPPPESEFKLASPEKLAGLASPSSGYSSQSETPTSSFPSAFFPGPLSPTSGKRKPKVPERKSSLCSLQQQQLSVRDPGISCRRETDFYAIPPSHLDLSALHSKHFPHRNQMHILHQNKQKAAIAAAAAAEARSAAAAAAEARSAAAAAAEARSAATAAAAAEACTAATASTKESATNTAPISAHLAITPTVLRSVQLRSICRPSDGNQGLDQDSSNLITRPKCPTIITDAPSSSNRHNRKPPAYKPPAAQVCDSQIPLVENIVFPQEEVRVRQERFGPGTYWAMTAFRTPVDPNCEKEVSLKRSSQCFQQEQDSRRCLDVQKTLSPERLKQDLNQLSRPDPSTQSMCLASTMPPPAYSEIQRSNFVLCNSPDKVPVSTQEASHSYTISDVQGREEDYETSGVATRSASQDIREEESTPDTEDYFSKDSTPSDNSSSPLTDDSRLDDDVFPSPNKLRTTEDLFAMIHRSKRKVLGRKDSGEVSGKGRPVVPPVTTPVSNPTVCPVIPAPPVPSNNSQRASGPIYRSAKKSSTSSEEFKLLLLKKGSRTDSSYRMSATEILKSPITPKTQAELLLEAMRQPEEIPLPQLDSTSSGDPLPSPFPKANSEGFSPKTLTMSAASRQGRSRIPPAANSSRYSTRSRLYTAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_021479858.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479858.1

ORF Insert Sequence:

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA 
GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT 
CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA 
CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC 
AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC 
CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGA 
CCAGCCAAGG ATTCTGAATT CAAACCTGTC CTCAGAAAGT ATCAGCGCTC CCGCTCCCCC 
TCCCCTGTTC AGTGTTGTTA CTTCATTCCC TGGATTAGAA AGGCTGGAAC CAACACAGAA 
ACGGATGGAG AACTACAAGT GATGAGCCAT AGGCCTAAAT GTCCTGTTCC CAATGCACCT 
ACCACCCTGG ACAAACAGAC TAACTGGTCT AAAGCCCTGC CTCTTCCCAC TCCAGAAGAA 
AGAATAAAGA ATGACTCTCA AGTGATTTCA TCCTGTATCA TTCCAATAAA TGTTTCGGGT 
GTTGGATTTG ACAGAGAAGC CAGTGCTCGC TGCTCTCTTG TTCACTCGCA ATCGGTTCTA 
CAGAGACGGC GGAAACTCAG GAGGCGAAAA ACGATCACTG GGATCCCCAA ACGAGTTCAA 
CAAGACATGG ATTCTGACGA ATCCCCTGTT GCTAGAGAAA GAACAGTGAT AATCCACGCC 
AATCCACACA AGTCCCACGA GTGGCATGAG GAGCTCTCCT TGAGTGGCAG AGTATTGCAT 
ACTAAGGATT CGGGCTGTCA GACAGATGAC TTCTTGATTA CTGCTCCATC CCGAAGACGC 
ATCCGTGCCC AAAGAGGTCA GGGAATTCCA GCGTCTCTCT CTCACTCCAC TGGAAACATT 
ACCTCCCTCC CGGATCGTTC TGATACGGTT TATGCTGCAG CATCAGCCAC TCGTGTCCGT 
TCTAGGAGCC TTCCACGTGA GGGTGGTCGG CTTCTGGATG AGGACCAAGA TGACAGTGAT 
GATGATGATG ATGAAGATGA TGATGAAGAT GATGAAGAGG AAGATGAGGA GCTCTCTCCA 
TATGAAGCCG AAGACTTCCT GCCAGGTCCC GGACAGAGAA TTCCAAAGGA TGAGGAAGAG 
AGTACTGACG ACCAAGCCAT GCCAGAGCTA CAGTTTGGAA GTCTTAAGCG TATGCAGCAT 
TCAGAGAGCC CTGACCACAC ATGGATTGAG AGAGGCAGAT CCAGACTCCC ACGGAAAGTA 
GACATGGGGA GCTGTGAGAT TTCCTCTAGT TCAGATACTT TCAGCAGCCC CATACACTCT 
GCATCCACTA CAGGAGTGCT TGGCAGCCAG ATTGACCATA AAGAGGATCA CCAGTCCTCA 
AGTGGCAACT GGAGTGGAAG CAGCTCCACT TGCCCATCGC AGACATCCGA GACTCTTCCA 
CCTGCTGCCT CCCCTCCCTT GACGGGATCC TCACACTGTG ATTCAGAACT TTCCCTGAAT 
ACTGGGTCCC ATGTCATAGA TGACAACACT GGTTTCATAA TTGATCCCTA TCACATAGAC 
AAGCCACAGG GACAGGGGCA ACGATCCAAT TCATTTACCT CATCAGCTAC TGATCAGATG 
GATGATGCAG GGGTCAGCAC TGCTAGTGAT GGGGAGTGGA ACTGTGCCCA GGACCAGCAG 
GATCAGTCCT GCACCCAAGA CTTCAGCCCA AAGCGTTCCA GAGAGGATGA GAGTGGTGTC 
AGCTGCCCTA GTTATTCTAG TGGGGAAACT TACGAGAGCT TCAGTGACCA AGCATCCGCT 
AGAAAATCTG AGATGCACTA CATTGTCGAC ACTGAAGGAT TCTATTCCTC CATGCACTTT 
GACTCTGGTC TTAAGGGTAG CAGAAGCTAC TACAACTATG CAGCCATTGA CCCCGACTGT 
GGCCCAAGTG GTAATATAGC ACCTGGCATG ACTGAATACC CTCAGCCAGA GTACAGAGCC 
ACCAGGACAC TGGGCAGACC CTGTCTCTCC TTAAGAAAAC CAAAGGCCAA GCCACCCCCA 
CCAAAACGTA GCTCTTCATT AAAGGAAACA AGCAGTGATG TGAACGTTCC AGAGCAGAAC 
GAACCAAAGA TTACTTGTGA GTTGCCACTG CCCTTGTCTT CCAGGGAGAT GAAACTGCAG 
CTGGACTTGG CTGATTCTGC AGGGCACCTT GAGACTGCAG GTCTTGAATC ACTTGGTGCA 
TGGGGAGTGG AAAATGTTAG GGACATGCTT GACTGCTCCT CCCCATTCAG TTCCTCAGAC 
ACACATTCCT TCAAAGATGA AGGTGCTGTG CAAGCAGACT ATGCAGACCT CTGGCTTCAC 
AATGACTTGA AATCAAATGA TCCTTATCGG TCCCTCTCTA ACTCTAGCTC TGCTACAGGT 
ACAACTGTTA TTGAATGCAT CAAGTCACCA GAAAGATCAG AAACGCACAC CTGTCAACCC 
AGGTCCAGAC CTTCTTCCCC AACTCTTCCT CCACCTGAAA GTGAATTTAA GCTTGCTTCT 
CCCGAAAAGC TGGCAGGCTT GGCGTCCCCT TCCAGTGGAT ATTCCAGTCA GTCTGAAACA 
CCTACATCTT CCTTCCCCTC TGCTTTCTTC CCAGGGCCCC TGTCTCCAAC CAGTGGGAAG 
AGGAAGCCCA AAGTCCCTGA AAGGAAGTCC TCACTTTGTT CCTTACAGCA GCAACAGCTT 
TCAGTCAGAG ACCCAGGCAT TTCCTGTAGG AGAGAAACTG ACTTCTATGC CATACCCCCA 
AGTCACCTTG ACCTAAGTGC TCTTCACAGT AAGCACTTTC CTCACAGAAA TCAAATGCAC 
ATCCTCCACC AGAATAAGCA GAAAGCTGCC ATAGCAGCAG CTGCTGCAGC AGAAGCTCGC 
AGTGCAGCAG CTGCTGCAGC AGAAGCTCGC AGTGCAGCAG CTGCTGCAGC AGAAGCTCGC 
AGTGCAGCAA CTGCAGCCGC TGCTGCCGAG GCCTGCACTG CAGCTACAGC TTCTACTAAA 
GAGAGTGCAA CAAATACAGC ACCCATCTCA GCCCATTTGG CTATTACTCC AACGGTTCTT 
AGATCAGTGC AACTGCGATC TATATGTAGA CCATCTGATG GCAACCAAGG GCTTGATCAA 
GACAGTTCAA ATCTCATAAC TCGTCCTAAG TGTCCCACAA TAATAACTGA TGCCCCATCA 
TCTAGCAACA GGCACAACAG GAAGCCACCA GCCTACAAAC CCCCTGCTGC ACAGGTTTGT 
GATTCACAGA TTCCACTGGT GGAAAACATT GTTTTTCCAC AAGAAGAAGT GAGAGTGAGA 
CAGGAGAGGT TTGGTCCTGG TACTTACTGG GCAATGACTG CTTTCAGAAC TCCTGTGGAC 
CCTAATTGTG AAAAAGAAGT CTCTTTAAAA AGGTCATCTC AGTGTTTTCA GCAAGAACAA 
GACAGCAGAA GGTGCCTAGA TGTGCAAAAG ACATTGTCAC CAGAGAGACT AAAACAAGAT 
TTGAATCAAC TATCGCGGCC AGACCCTTCA ACACAGTCCA TGTGTTTGGC CAGCACAATG 
CCACCCCCTG CTTATAGTGA GATACAGAGG AGCAATTTTG TACTGTGCAA CAGTCCTGAC 
AAAGTGCCTG TTTCAACTCA AGAGGCATCG CATTCTTACA CAATCAGCGA TGTACAAGGC 
AGAGAGGAGG ATTATGAGAC ATCAGGTGTC GCAACCAGAA GTGCCTCACA GGACATAAGG 
GAAGAAGAGT CAACCCCAGA TACAGAGGAC TATTTCAGTA AAGACTCTAC TCCCAGTGAT 
AATTCATCCT CTCCTTTGAC CGATGACTCC AGACTTGATG ATGATGTTTT CCCATCTCCC 
AATAAACTCC GCACAACTGA AGATCTTTTT GCTATGATAC ACAGATCTAA AAGGAAAGTG 
CTGGGACGCA AAGATTCAGG GGAAGTCAGT GGAAAAGGTC GGCCTGTTGT ACCACCTGTG 
ACCACCCCTG TAAGCAATCC TACTGTATGC CCGGTCATCC CTGCTCCCCC AGTTCCTTCA 
AACAACTCCC AGCGAGCCTC AGGACCCATC TACAGGAGTG CCAAAAAGTC TAGTACATCC 
AGTGAGGAGT TCAAACTCCT TCTGCTTAAG AAGGGTAGTC GCACAGACTC AAGTTATCGC 
ATGTCTGCTA CAGAGATCCT TAAGAGCCCC ATCACACCCA AGACTCAAGC AGAGCTGCTG 
TTAGAGGCCA TGAGGCAACC AGAGGAGATC CCCTTACCCC AACTGGATTC CACCAGCAGT 
GGAGATCCAC TTCCAAGTCC ATTCCCTAAG GCCAACAGTG AGGGATTCTC CCCAAAAACA 
CTCACCATGT CAGCTGCCTC CAGACAAGGA CGTTCTAGAA TTCCACCTGC AGCGAACAGC 
AGTCGCTATA GCACGCGGAG TCGGCTGTAC ACTGCCCCAA TGCAGGCCAT ATCAGAAGGA 
GAGACTGAGA ACTCGGATGG AAGTCCACAC GATGACCGCT CCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsb ( XM_021479859.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335534.1 Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X6, mRNA.

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CloneID	ODa148026
Clone ID Related Accession (Same CDS sequence)	XM_021479859.1
Accession Version	XM_021479859.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Nance-Horan syndrome protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA 
GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT 
CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA 
CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC 
AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC 
CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGA 
CCAGCCAAGG ATTCTGAATT CAAACCTGTC CTCAGAAAGG CTGGAACCAA CACAGAAACG 
GATGGAGAAC TACAAGTGAT GAGCCATAGG CCTAAATGTC CTGTTCCCAA TGCACCTACC 
ACCCTGGACA AACAGACTAA CTGGTCTAAA GCCCTGCCTC TTCCCACTCC AGAAGAAAGA 
ATAAAGAATG ACTCTCAAGT GATTTCATCC TGTATCATTC CAATAAATGT TTCGGGTGTT 
GGATTTGACA GAGAAGCCAG TGCTCGCTGC TCTCTTGTTC ACTCGCAATC GGTTCTACAG 
AGACGGCGGA AACTCAGGAG GCGAAAAACG ATCACTGGGA TCCCCAAACG AGTTCAACAA 
GACATGGATT CTGACGAATC CCCTGTTGCT AGAGAAAGAA CAGTGATAAT CCACGCCAAT 
CCACACAAGT CCCACGAGTG GCATGAGGAG CTCTCCTTGA GTGGCAGAGT ATTGCATACT 
AAGGATTCGG GCTGTCAGAC AGATGACTTC TTGATTACTG CTCCATCCCG AAGACGCATC 
CGTGCCCAAA GAGGTCAGGG AATTCCAGCG TCTCTCTCTC ACTCCACTGG AAACATTACC 
TCCCTCCCGG ATCGTTCTGA TACGGTTTAT GCTGCAGCAT CAGCCACTCG TGTCCGTTCT 
AGGAGCCTTC CACGTGAGGG TGGTCGGCTT CTGGATGAGG ACCAAGATGA CAGTGATGAT 
GATGATGATG AAGATGATGA TGAAGATGAT GAAGAGGAAG ATGAGGAGCT CTCTCCATAT 
GAAGCCGAAG ACTTCCTGCC AGGTCCCGGA CAGAGAATTC CAAAGGATGA GGAAGAGAGT 
ACTGACGACC AAGCCATGCC AGAGCTACAG TTTGGAAGTC TTAAGCGTAT GCAGCATTCA 
GAGAGCCCTG ACCACACATG GATTGAGAGA GGCAGATCCA GACTCCCACG GAAAGTAGAC 
ATGGGGAGCT GTGAGATTTC CTCTAGTTCA GATACTTTCA GCAGCCCCAT ACACTCTGCA 
TCCACTACAG GAGTGCTTGG CAGCCAGATT GACCATAAAG AGGATCACCA GTCCTCAAGT 
GGCAACTGGA GTGGAAGCAG CTCCACTTGC CCATCGCAGA CATCCGAGAC TCTTCCACCT 
GCTGCCTCCC CTCCCTTGAC GGGATCCTCA CACTGTGATT CAGAACTTTC CCTGAATACT 
GGGTCCCATG TCATAGATGA CAACACTGGT TTCATAATTG ATCCCTATCA CATAGACAAG 
CCACAGGGAC AGGGGCAACG ATCCAATTCA TTTACCTCAT CAGCTACTGA TCAGATGGAT 
GATGCAGGGG TCAGCACTGC TAGTGATGGG GAGTGGAACT GTGCCCAGGA CCAGCAGGAT 
CAGTCCTGCA CCCAAGACTT CAGCCCAAAG CGTTCCAGAG AGGATGAGAG TGGTGTCAGC 
TGCCCTAGTT ATTCTAGTGG GGAAACTTAC GAGAGCTTCA GTGACCAAGC ATCCGCTAGA 
AAATCTGAGA TGCACTACAT TGTCGACACT GAAGGATTCT ATTCCTCCAT GCACTTTGAC 
TCTGGTCTTA AGGGTAGCAG AAGCTACTAC AACTATGCAG CCATTGACCC CGACTGTGGC 
CCAAGTGGTA ATATAGCACC TGGCATGACT GAATACCCTC AGCCAGAGTA CAGAGCCACC 
AGGACACTGG GCAGACCCTG TCTCTCCTTA AGAAAACCAA AGGCCAAGCC ACCCCCACCA 
AAACGTAGCT CTTCATTAAA GGAAACAAGC AGTGATGTGA ACGTTCCAGA GCAGAACGAA 
CCAAAGATTA CTTGTGAGTT GCCACTGCCC TTGTCTTCCA GGGAGATGAA ACTGCAGCTG 
GACTTGGCTG ATTCTGCAGG GCACCTTGAG ACTGCAGGTC TTGAATCACT TGGTGCATGG 
GGAGTGGAAA ATGTTAGGGA CATGCTTGAC TGCTCCTCCC CATTCAGTTC CTCAGACACA 
CATTCCTTCA AAGATGAAGG TGCTGTGCAA GCAGACTATG CAGACCTCTG GCTTCACAAT 
GACTTGAAAT CAAATGATCC TTATCGGTCC CTCTCTAACT CTAGCTCTGC TACAGGTACA 
ACTGTTATTG AATGCATCAA GTCACCAGAA AGATCAGAAA CGCACACCTG TCAACCCAGG 
TCCAGACCTT CTTCCCCAAC TCTTCCTCCA CCTGAAAGTG AATTTAAGCT TGCTTCTCCC 
GAAAAGCTGG CAGGCTTGGC GTCCCCTTCC AGTGGATATT CCAGTCAGTC TGAAACACCT 
ACATCTTCCT TCCCCTCTGC TTTCTTCCCA GGGCCCCTGT CTCCAACCAG TGGGAAGAGG 
AAGCCCAAAG TCCCTGAAAG GAAGTCCTCA CTTTGTTCCT TACAGCAGCA ACAGCTTTCA 
GTCAGAGACC CAGGCATTTC CTGTAGGAGA GAAACTGACT TCTATGCCAT ACCCCCAAGT 
CACCTTGACC TAAGTGCTCT TCACAGTAAG CACTTTCCTC ACAGAAATCA AATGCACATC 
CTCCACCAGA ATAAGCAGAA AGCTGCCATA GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT 
GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT 
GCAGCAACTG CAGCCGCTGC TGCCGAGGCC TGCACTGCAG CTACAGCTTC TACTAAAGAG 
AGTGCAACAA ATACAGCACC CATCTCAGCC CATTTGGCTA TTACTCCAAC GGTTCTTAGA 
TCAGTGCAAC TGCGATCTAT ATGTAGACCA TCTGATGGCA ACCAAGGGCT TGATCAAGAC 
AGTTCAAATC TCATAACTCG TCCTAAGTGT CCCACAATAA TAACTGATGC CCCATCATCT 
AGCAACAGGC ACAACAGGAA GCCACCAGCC TACAAACCCC CTGCTGCACA GGTTTGTGAT 
TCACAGATTC CACTGGTGGA AAACATTGTT TTTCCACAAG AAGAAGTGAG AGTGAGACAG 
GAGAGGTTTG GTCCTGGTAC TTACTGGGCA ATGACTGCTT TCAGAACTCC TGTGGACCCT 
AATTGTGAAA AAGAAGTCTC TTTAAAAAGG TCATCTCAGT GTTTTCAGCA AGAACAAGAC 
AGCAGAAGGT GCCTAGATGT GCAAAAGACA TTGTCACCAG AGAGACTAAA ACAAGATTTG 
AATCAACTAT CGCGGCCAGA CCCTTCAACA CAGTCCATGT GTTTGGCCAG CACAATGCCA 
CCCCCTGCTT ATAGTGAGAT ACAGAGGAGC AATTTTGTAC TGTGCAACAG TCCTGACAAA 
GTGCCTGTTT CAACTCAAGA GGCATCGCAT TCTTACACAA TCAGCGATGT ACAAGGCAGA 
GAGGAGGATT ATGAGACATC AGGTGTCGCA ACCAGAAGTG CCTCACAGGA CATAAGGGAA 
GAAGAGTCAA CCCCAGATAC AGAGGACTAT TTCAGTAAAG ACTCTACTCC CAGTGATAAT 
TCATCCTCTC CTTTGACCGA TGACTCCAGA CTTGATGATG ATGTTTTCCC ATCTCCCAAT 
AAACTCCGCA CAACTGAAGA TCTTTTTGCT ATGATACACA GATCTAAAAG GAAAGTGCTG 
GGACGCAAAG ATTCAGGGGA AGTCAGTGGA AAAGGTCGGC CTGTTGTACC ACCTGTGACC 
ACCCCTGTAA GCAATCCTAC TGTATGCCCG GTCATCCCTG CTCCCCCAGT TCCTTCAAAC 
AACTCCCAGC GAGCCTCAGG ACCCATCTAC AGGAGTGCCA AAAAGTCTAG TACATCCAGT 
GAGGAGTTCA AACTCCTTCT GCTTAAGAAG GGTAGTCGCA CAGACTCAAG TTATCGCATG 
TCTGCTACAG AGATCCTTAA GAGCCCCATC ACACCCAAGA CTCAAGCAGA GCTGCTGTTA 
GAGGCCATGA GGCAACCAGA GGAGATCCCC TTACCCCAAC TGGATTCCAC CAGCAGTGGA 
GATCCACTTC CAAGTCCATT CCCTAAGGCC AACAGTGAGG GATTCTCCCC AAAAACACTC 
ACCATGTCAG CTGCCTCCAG ACAAGGACGT TCTAGAATTC CACCTGCAGC GAACAGCAGT 
CGCTATAGCA CGCGGAGTCG GCTGTACACT GCCCCAATGC AGGCCATATC AGAAGGAGAG 
ACTGAGAACT CGGATGGAAG TCCACACGAT GACCGCTCCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335534.1
CDS	320..5104
Translation	MPFAKRIVEPQLLVRHPIPNDEGLLFEDLCSITNVALSRTLRQLSDLSKHACSLFQELENEIVSTNQRVWALQNKIGKIQQTASALDPKMEAVRFRSISIAVSNLDIESKLTSHYQAPWHQQHNLFHTCTRPACLEELHRHAKLNLRALHRDQQQRQRSTSRERSRVTISISVAPPMPTFPSTHKLRRREQRGRHSRMERPAKDSEFKPVLRKAGTNTETDGELQVMSHRPKCPVPNAPTTLDKQTNWSKALPLPTPEERIKNDSQVISSCIIPINVSGVGFDREASARCSLVHSQSVLQRRRKLRRRKTITGIPKRVQQDMDSDESPVARERTVIIHANPHKSHEWHEELSLSGRVLHTKDSGCQTDDFLITAPSRRRIRAQRGQGIPASLSHSTGNITSLPDRSDTVYAAASATRVRSRSLPREGGRLLDEDQDDSDDDDDEDDDEDDEEEDEELSPYEAEDFLPGPGQRIPKDEEESTDDQAMPELQFGSLKRMQHSESPDHTWIERGRSRLPRKVDMGSCEISSSSDTFSSPIHSASTTGVLGSQIDHKEDHQSSSGNWSGSSSTCPSQTSETLPPAASPPLTGSSHCDSELSLNTGSHVIDDNTGFIIDPYHIDKPQGQGQRSNSFTSSATDQMDDAGVSTASDGEWNCAQDQQDQSCTQDFSPKRSREDESGVSCPSYSSGETYESFSDQASARKSEMHYIVDTEGFYSSMHFDSGLKGSRSYYNYAAIDPDCGPSGNIAPGMTEYPQPEYRATRTLGRPCLSLRKPKAKPPPPKRSSSLKETSSDVNVPEQNEPKITCELPLPLSSREMKLQLDLADSAGHLETAGLESLGAWGVENVRDMLDCSSPFSSSDTHSFKDEGAVQADYADLWLHNDLKSNDPYRSLSNSSSATGTTVIECIKSPERSETHTCQPRSRPSSPTLPPPESEFKLASPEKLAGLASPSSGYSSQSETPTSSFPSAFFPGPLSPTSGKRKPKVPERKSSLCSLQQQQLSVRDPGISCRRETDFYAIPPSHLDLSALHSKHFPHRNQMHILHQNKQKAAIAAAAAAEARSAAAAAAEARSAAAAAAEARSAATAAAAAEACTAATASTKESATNTAPISAHLAITPTVLRSVQLRSICRPSDGNQGLDQDSSNLITRPKCPTIITDAPSSSNRHNRKPPAYKPPAAQVCDSQIPLVENIVFPQEEVRVRQERFGPGTYWAMTAFRTPVDPNCEKEVSLKRSSQCFQQEQDSRRCLDVQKTLSPERLKQDLNQLSRPDPSTQSMCLASTMPPPAYSEIQRSNFVLCNSPDKVPVSTQEASHSYTISDVQGREEDYETSGVATRSASQDIREEESTPDTEDYFSKDSTPSDNSSSPLTDDSRLDDDVFPSPNKLRTTEDLFAMIHRSKRKVLGRKDSGEVSGKGRPVVPPVTTPVSNPTVCPVIPAPPVPSNNSQRASGPIYRSAKKSSTSSEEFKLLLLKKGSRTDSSYRMSATEILKSPITPKTQAELLLEAMRQPEEIPLPQLDSTSSGDPLPSPFPKANSEGFSPKTLTMSAASRQGRSRIPPAANSSRYSTRSRLYTAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_021479859.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479859.1

ORF Insert Sequence:

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA 
GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT 
CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA 
CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC 
AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC 
CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGA 
CCAGCCAAGG ATTCTGAATT CAAACCTGTC CTCAGAAAGG CTGGAACCAA CACAGAAACG 
GATGGAGAAC TACAAGTGAT GAGCCATAGG CCTAAATGTC CTGTTCCCAA TGCACCTACC 
ACCCTGGACA AACAGACTAA CTGGTCTAAA GCCCTGCCTC TTCCCACTCC AGAAGAAAGA 
ATAAAGAATG ACTCTCAAGT GATTTCATCC TGTATCATTC CAATAAATGT TTCGGGTGTT 
GGATTTGACA GAGAAGCCAG TGCTCGCTGC TCTCTTGTTC ACTCGCAATC GGTTCTACAG 
AGACGGCGGA AACTCAGGAG GCGAAAAACG ATCACTGGGA TCCCCAAACG AGTTCAACAA 
GACATGGATT CTGACGAATC CCCTGTTGCT AGAGAAAGAA CAGTGATAAT CCACGCCAAT 
CCACACAAGT CCCACGAGTG GCATGAGGAG CTCTCCTTGA GTGGCAGAGT ATTGCATACT 
AAGGATTCGG GCTGTCAGAC AGATGACTTC TTGATTACTG CTCCATCCCG AAGACGCATC 
CGTGCCCAAA GAGGTCAGGG AATTCCAGCG TCTCTCTCTC ACTCCACTGG AAACATTACC 
TCCCTCCCGG ATCGTTCTGA TACGGTTTAT GCTGCAGCAT CAGCCACTCG TGTCCGTTCT 
AGGAGCCTTC CACGTGAGGG TGGTCGGCTT CTGGATGAGG ACCAAGATGA CAGTGATGAT 
GATGATGATG AAGATGATGA TGAAGATGAT GAAGAGGAAG ATGAGGAGCT CTCTCCATAT 
GAAGCCGAAG ACTTCCTGCC AGGTCCCGGA CAGAGAATTC CAAAGGATGA GGAAGAGAGT 
ACTGACGACC AAGCCATGCC AGAGCTACAG TTTGGAAGTC TTAAGCGTAT GCAGCATTCA 
GAGAGCCCTG ACCACACATG GATTGAGAGA GGCAGATCCA GACTCCCACG GAAAGTAGAC 
ATGGGGAGCT GTGAGATTTC CTCTAGTTCA GATACTTTCA GCAGCCCCAT ACACTCTGCA 
TCCACTACAG GAGTGCTTGG CAGCCAGATT GACCATAAAG AGGATCACCA GTCCTCAAGT 
GGCAACTGGA GTGGAAGCAG CTCCACTTGC CCATCGCAGA CATCCGAGAC TCTTCCACCT 
GCTGCCTCCC CTCCCTTGAC GGGATCCTCA CACTGTGATT CAGAACTTTC CCTGAATACT 
GGGTCCCATG TCATAGATGA CAACACTGGT TTCATAATTG ATCCCTATCA CATAGACAAG 
CCACAGGGAC AGGGGCAACG ATCCAATTCA TTTACCTCAT CAGCTACTGA TCAGATGGAT 
GATGCAGGGG TCAGCACTGC TAGTGATGGG GAGTGGAACT GTGCCCAGGA CCAGCAGGAT 
CAGTCCTGCA CCCAAGACTT CAGCCCAAAG CGTTCCAGAG AGGATGAGAG TGGTGTCAGC 
TGCCCTAGTT ATTCTAGTGG GGAAACTTAC GAGAGCTTCA GTGACCAAGC ATCCGCTAGA 
AAATCTGAGA TGCACTACAT TGTCGACACT GAAGGATTCT ATTCCTCCAT GCACTTTGAC 
TCTGGTCTTA AGGGTAGCAG AAGCTACTAC AACTATGCAG CCATTGACCC CGACTGTGGC 
CCAAGTGGTA ATATAGCACC TGGCATGACT GAATACCCTC AGCCAGAGTA CAGAGCCACC 
AGGACACTGG GCAGACCCTG TCTCTCCTTA AGAAAACCAA AGGCCAAGCC ACCCCCACCA 
AAACGTAGCT CTTCATTAAA GGAAACAAGC AGTGATGTGA ACGTTCCAGA GCAGAACGAA 
CCAAAGATTA CTTGTGAGTT GCCACTGCCC TTGTCTTCCA GGGAGATGAA ACTGCAGCTG 
GACTTGGCTG ATTCTGCAGG GCACCTTGAG ACTGCAGGTC TTGAATCACT TGGTGCATGG 
GGAGTGGAAA ATGTTAGGGA CATGCTTGAC TGCTCCTCCC CATTCAGTTC CTCAGACACA 
CATTCCTTCA AAGATGAAGG TGCTGTGCAA GCAGACTATG CAGACCTCTG GCTTCACAAT 
GACTTGAAAT CAAATGATCC TTATCGGTCC CTCTCTAACT CTAGCTCTGC TACAGGTACA 
ACTGTTATTG AATGCATCAA GTCACCAGAA AGATCAGAAA CGCACACCTG TCAACCCAGG 
TCCAGACCTT CTTCCCCAAC TCTTCCTCCA CCTGAAAGTG AATTTAAGCT TGCTTCTCCC 
GAAAAGCTGG CAGGCTTGGC GTCCCCTTCC AGTGGATATT CCAGTCAGTC TGAAACACCT 
ACATCTTCCT TCCCCTCTGC TTTCTTCCCA GGGCCCCTGT CTCCAACCAG TGGGAAGAGG 
AAGCCCAAAG TCCCTGAAAG GAAGTCCTCA CTTTGTTCCT TACAGCAGCA ACAGCTTTCA 
GTCAGAGACC CAGGCATTTC CTGTAGGAGA GAAACTGACT TCTATGCCAT ACCCCCAAGT 
CACCTTGACC TAAGTGCTCT TCACAGTAAG CACTTTCCTC ACAGAAATCA AATGCACATC 
CTCCACCAGA ATAAGCAGAA AGCTGCCATA GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT 
GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT 
GCAGCAACTG CAGCCGCTGC TGCCGAGGCC TGCACTGCAG CTACAGCTTC TACTAAAGAG 
AGTGCAACAA ATACAGCACC CATCTCAGCC CATTTGGCTA TTACTCCAAC GGTTCTTAGA 
TCAGTGCAAC TGCGATCTAT ATGTAGACCA TCTGATGGCA ACCAAGGGCT TGATCAAGAC 
AGTTCAAATC TCATAACTCG TCCTAAGTGT CCCACAATAA TAACTGATGC CCCATCATCT 
AGCAACAGGC ACAACAGGAA GCCACCAGCC TACAAACCCC CTGCTGCACA GGTTTGTGAT 
TCACAGATTC CACTGGTGGA AAACATTGTT TTTCCACAAG AAGAAGTGAG AGTGAGACAG 
GAGAGGTTTG GTCCTGGTAC TTACTGGGCA ATGACTGCTT TCAGAACTCC TGTGGACCCT 
AATTGTGAAA AAGAAGTCTC TTTAAAAAGG TCATCTCAGT GTTTTCAGCA AGAACAAGAC 
AGCAGAAGGT GCCTAGATGT GCAAAAGACA TTGTCACCAG AGAGACTAAA ACAAGATTTG 
AATCAACTAT CGCGGCCAGA CCCTTCAACA CAGTCCATGT GTTTGGCCAG CACAATGCCA 
CCCCCTGCTT ATAGTGAGAT ACAGAGGAGC AATTTTGTAC TGTGCAACAG TCCTGACAAA 
GTGCCTGTTT CAACTCAAGA GGCATCGCAT TCTTACACAA TCAGCGATGT ACAAGGCAGA 
GAGGAGGATT ATGAGACATC AGGTGTCGCA ACCAGAAGTG CCTCACAGGA CATAAGGGAA 
GAAGAGTCAA CCCCAGATAC AGAGGACTAT TTCAGTAAAG ACTCTACTCC CAGTGATAAT 
TCATCCTCTC CTTTGACCGA TGACTCCAGA CTTGATGATG ATGTTTTCCC ATCTCCCAAT 
AAACTCCGCA CAACTGAAGA TCTTTTTGCT ATGATACACA GATCTAAAAG GAAAGTGCTG 
GGACGCAAAG ATTCAGGGGA AGTCAGTGGA AAAGGTCGGC CTGTTGTACC ACCTGTGACC 
ACCCCTGTAA GCAATCCTAC TGTATGCCCG GTCATCCCTG CTCCCCCAGT TCCTTCAAAC 
AACTCCCAGC GAGCCTCAGG ACCCATCTAC AGGAGTGCCA AAAAGTCTAG TACATCCAGT 
GAGGAGTTCA AACTCCTTCT GCTTAAGAAG GGTAGTCGCA CAGACTCAAG TTATCGCATG 
TCTGCTACAG AGATCCTTAA GAGCCCCATC ACACCCAAGA CTCAAGCAGA GCTGCTGTTA 
GAGGCCATGA GGCAACCAGA GGAGATCCCC TTACCCCAAC TGGATTCCAC CAGCAGTGGA 
GATCCACTTC CAAGTCCATT CCCTAAGGCC AACAGTGAGG GATTCTCCCC AAAAACACTC 
ACCATGTCAG CTGCCTCCAG ACAAGGACGT TCTAGAATTC CACCTGCAGC GAACAGCAGT 
CGCTATAGCA CGCGGAGTCG GCTGTACACT GCCCCAATGC AGGCCATATC AGAAGGAGAG 
ACTGAGAACT CGGATGGAAG TCCACACGAT GACCGCTCCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsb ( XM_021479860.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335535.1 Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X7, mRNA.

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CloneID	ODa148027
Clone ID Related Accession (Same CDS sequence)	XM_021479860.1
Accession Version	XM_021479860.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4629bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Nance-Horan syndrome protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTTG CCTGCTGTGT TCCTGTCAGA TCGAGAGCGG TCTCGAATCT GGACATTGAG 
AGTAAACTGA CGTCGCACTA TCAGGCTCCA TGGCATCAGC AACACAATCT GTTCCACACT 
TGCACACGGC CAGCGTGTCT GGAGGAGCTG CACCGACATG CCAAGCTCAA CCTGCGGGCG 
CTGCACAGAG ACCAGCAGCA GCGTCAGCGT TCAACCAGCA GAGAGCGCAG TCGGGTCACT 
ATATCAATAT CTGTGGCACC GCCAATGCCA ACCTTCCCTT CAACTCACAA ACTCAGACGG 
CGAGAGCAGA GAGGTCGACA TTCACGCATG GAGAGGAGCG TTAGAGAGAG TGATGTGCAA 
ACTATCCAGA GGAAGGAGAG ACCAGCCAAG GATTCTGAAT TCAAACCTGT CCTCAGAAAG 
TATCAGCGCT CCCGCTCCCC CTCCCCTGTT CAGTGTTGTT ACTTCATTCC CTGGATTAGA 
AAGGCTGGAA CCAACACAGA AACGGATGGA GAACTACAAG TGATGAGCCA TAGGCCTAAA 
TGTCCTGTTC CCAATGCACC TACCACCCTG GACAAACAGA CTAACTGGTC TAAAGCCCTG 
CCTCTTCCCA CTCCAGAAGA AAGAATAAAG AATGACTCTC AAGTGATTTC ATCCTGTATC 
ATTCCAATAA ATGTTTCGGG TGTTGGATTT GACAGAGAAG CCAGTGCTCG CTGCTCTCTT 
GTTCACTCGC AATCGGTTCT ACAGAGACGG CGGAAACTCA GGAGGCGAAA AACGATCACT 
GGGATCCCCA AACGAGTTCA ACAAGACATG GATTCTGACG AATCCCCTGT TGCTAGAGAA 
AGAACAGTGA TAATCCACGC CAATCCACAC AAGTCCCACG AGTGGCATGA GGAGCTCTCC 
TTGAGTGGCA GAGTATTGCA TACTAAGGAT TCGGGCTGTC AGACAGATGA CTTCTTGATT 
ACTGCTCCAT CCCGAAGACG CATCCGTGCC CAAAGAGGTC AGGGAATTCC AGCGTCTCTC 
TCTCACTCCA CTGGAAACAT TACCTCCCTC CCGGATCGTT CTGATACGGT TTATGCTGCA 
GCATCAGCCA CTCGTGTCCG TTCTAGGAGC CTTCCACGTG AGGGTGGTCG GCTTCTGGAT 
GAGGACCAAG ATGACAGTGA TGATGATGAT GATGAAGATG ATGATGAAGA TGATGAAGAG 
GAAGATGAGG AGCTCTCTCC ATATGAAGCC GAAGACTTCC TGCCAGGTCC CGGACAGAGA 
ATTCCAAAGG ATGAGGAAGA GAGTACTGAC GACCAAGCCA TGCCAGAGCT ACAGTTTGGA 
AGTCTTAAGC GTATGCAGCA TTCAGAGAGC CCTGACCACA CATGGATTGA GAGAGGCAGA 
TCCAGACTCC CACGGAAAGT AGACATGGGG AGCTGTGAGA TTTCCTCTAG TTCAGATACT 
TTCAGCAGCC CCATACACTC TGCATCCACT ACAGGAGTGC TTGGCAGCCA GATTGACCAT 
AAAGAGGATC ACCAGTCCTC AAGTGGCAAC TGGAGTGGAA GCAGCTCCAC TTGCCCATCG 
CAGACATCCG AGACTCTTCC ACCTGCTGCC TCCCCTCCCT TGACGGGATC CTCACACTGT 
GATTCAGAAC TTTCCCTGAA TACTGGGTCC CATGTCATAG ATGACAACAC TGGTTTCATA 
ATTGATCCCT ATCACATAGA CAAGCCACAG GGACAGGGGC AACGATCCAA TTCATTTACC 
TCATCAGCTA CTGATCAGAT GGATGATGCA GGGGTCAGCA CTGCTAGTGA TGGGGAGTGG 
AACTGTGCCC AGGACCAGCA GGATCAGTCC TGCACCCAAG ACTTCAGCCC AAAGCGTTCC 
AGAGAGGATG AGAGTGGTGT CAGCTGCCCT AGTTATTCTA GTGGGGAAAC TTACGAGAGC 
TTCAGTGACC AAGCATCCGC TAGAAAATCT GAGATGCACT ACATTGTCGA CACTGAAGGA 
TTCTATTCCT CCATGCACTT TGACTCTGGT CTTAAGGGTA GCAGAAGCTA CTACAACTAT 
GCAGCCATTG ACCCCGACTG TGGCCCAAGT GGTAATATAG CACCTGGCAT GACTGAATAC 
CCTCAGCCAG AGTACAGAGC CACCAGGACA CTGGGCAGAC CCTGTCTCTC CTTAAGAAAA 
CCAAAGGCCA AGCCACCCCC ACCAAAACGT AGCTCTTCAT TAAAGGAAAC AAGCAGTGAT 
GTGAACGTTC CAGAGCAGAA CGAACCAAAG ATTACTTGTG AGTTGCCACT GCCCTTGTCT 
TCCAGGGAGA TGAAACTGCA GCTGGACTTG GCTGATTCTG CAGGGCACCT TGAGACTGCA 
GGTCTTGAAT CACTTGGTGC ATGGGGAGTG GAAAATGTTA GGGACATGCT TGACTGCTCC 
TCCCCATTCA GTTCCTCAGA CACACATTCC TTCAAAGATG AAGGTGCTGT GCAAGCAGAC 
TATGCAGACC TCTGGCTTCA CAATGACTTG AAATCAAATG ATCCTTATCG GTCCCTCTCT 
AACTCTAGCT CTGCTACAGG TACAACTGTT ATTGAATGCA TCAAGTCACC AGAAAGATCA 
GAAACGCACA CCTGTCAACC CAGGTCCAGA CCTTCTTCCC CAACTCTTCC TCCACCTGAA 
AGTGAATTTA AGCTTGCTTC TCCCGAAAAG CTGGCAGGCT TGGCGTCCCC TTCCAGTGGA 
TATTCCAGTC AGTCTGAAAC ACCTACATCT TCCTTCCCCT CTGCTTTCTT CCCAGGGCCC 
CTGTCTCCAA CCAGTGGGAA GAGGAAGCCC AAAGTCCCTG AAAGGAAGTC CTCACTTTGT 
TCCTTACAGC AGCAACAGCT TTCAGTCAGA GACCCAGGCA TTTCCTGTAG GAGAGAAACT 
GACTTCTATG CCATACCCCC AAGTCACCTT GACCTAAGTG CTCTTCACAG TAAGCACTTT 
CCTCACAGAA ATCAAATGCA CATCCTCCAC CAGAATAAGC AGAAAGCTGC CATAGCAGCA 
GCTGCTGCAG CAGAAGCTCG CAGTGCAGCA GCTGCTGCAG CAGAAGCTCG CAGTGCAGCA 
GCTGCTGCAG CAGAAGCTCG CAGTGCAGCA ACTGCAGCCG CTGCTGCCGA GGCCTGCACT 
GCAGCTACAG CTTCTACTAA AGAGAGTGCA ACAAATACAG CACCCATCTC AGCCCATTTG 
GCTATTACTC CAACGGTTCT TAGATCAGTG CAACTGCGAT CTATATGTAG ACCATCTGAT 
GGCAACCAAG GGCTTGATCA AGACAGTTCA AATCTCATAA CTCGTCCTAA GTGTCCCACA 
ATAATAACTG ATGCCCCATC ATCTAGCAAC AGGCACAACA GGAAGCCACC AGCCTACAAA 
CCCCCTGCTG CACAGGTTTG TGATTCACAG ATTCCACTGG TGGAAAACAT TGTTTTTCCA 
CAAGAAGAAG TGAGAGTGAG ACAGGAGAGG TTTGGTCCTG GTACTTACTG GGCAATGACT 
GCTTTCAGAA CTCCTGTGGA CCCTAATTGT GAAAAAGAAG TCTCTTTAAA AAGGTCATCT 
CAGTGTTTTC AGCAAGAACA AGACAGCAGA AGGTGCCTAG ATGTGCAAAA GACATTGTCA 
CCAGAGAGAC TAAAACAAGA TTTGAATCAA CTATCGCGGC CAGACCCTTC AACACAGTCC 
ATGTGTTTGG CCAGCACAAT GCCACCCCCT GCTTATAGTG AGATACAGAG GAGCAATTTT 
GTACTGTGCA ACAGTCCTGA CAAAGTGCCT GTTTCAACTC AAGAGGCATC GCATTCTTAC 
ACAATCAGCG ATGTACAAGG CAGAGAGGAG GATTATGAGA CATCAGGTGT CGCAACCAGA 
AGTGCCTCAC AGGACATAAG GGAAGAAGAG TCAACCCCAG ATACAGAGGA CTATTTCAGT 
AAAGACTCTA CTCCCAGTGA TAATTCATCC TCTCCTTTGA CCGATGACTC CAGACTTGAT 
GATGATGTTT TCCCATCTCC CAATAAACTC CGCACAACTG AAGATCTTTT TGCTATGATA 
CACAGATCTA AAAGGAAAGT GCTGGGACGC AAAGATTCAG GGGAAGTCAG TGGAAAAGGT 
CGGCCTGTTG TACCACCTGT GACCACCCCT GTAAGCAATC CTACTGTATG CCCGGTCATC 
CCTGCTCCCC CAGTTCCTTC AAACAACTCC CAGCGAGCCT CAGGACCCAT CTACAGGAGT 
GCCAAAAAGT CTAGTACATC CAGTGAGGAG TTCAAACTCC TTCTGCTTAA GAAGGGTAGT 
CGCACAGACT CAAGTTATCG CATGTCTGCT ACAGAGATCC TTAAGAGCCC CATCACACCC 
AAGACTCAAG CAGAGCTGCT GTTAGAGGCC ATGAGGCAAC CAGAGGAGAT CCCCTTACCC 
CAACTGGATT CCACCAGCAG TGGAGATCCA CTTCCAAGTC CATTCCCTAA GGCCAACAGT 
GAGGGATTCT CCCCAAAAAC ACTCACCATG TCAGCTGCCT CCAGACAAGG ACGTTCTAGA 
ATTCCACCTG CAGCGAACAG CAGTCGCTAT AGCACGCGGA GTCGGCTGTA CACTGCCCCA 
ATGCAGGCCA TATCAGAAGG AGAGACTGAG AACTCGGATG GAAGTCCACA CGATGACCGC 
TCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335535.1
CDS	195..4823
Translation	MAFACCVPVRSRAVSNLDIESKLTSHYQAPWHQQHNLFHTCTRPACLEELHRHAKLNLRALHRDQQQRQRSTSRERSRVTISISVAPPMPTFPSTHKLRRREQRGRHSRMERSVRESDVQTIQRKERPAKDSEFKPVLRKYQRSRSPSPVQCCYFIPWIRKAGTNTETDGELQVMSHRPKCPVPNAPTTLDKQTNWSKALPLPTPEERIKNDSQVISSCIIPINVSGVGFDREASARCSLVHSQSVLQRRRKLRRRKTITGIPKRVQQDMDSDESPVARERTVIIHANPHKSHEWHEELSLSGRVLHTKDSGCQTDDFLITAPSRRRIRAQRGQGIPASLSHSTGNITSLPDRSDTVYAAASATRVRSRSLPREGGRLLDEDQDDSDDDDDEDDDEDDEEEDEELSPYEAEDFLPGPGQRIPKDEEESTDDQAMPELQFGSLKRMQHSESPDHTWIERGRSRLPRKVDMGSCEISSSSDTFSSPIHSASTTGVLGSQIDHKEDHQSSSGNWSGSSSTCPSQTSETLPPAASPPLTGSSHCDSELSLNTGSHVIDDNTGFIIDPYHIDKPQGQGQRSNSFTSSATDQMDDAGVSTASDGEWNCAQDQQDQSCTQDFSPKRSREDESGVSCPSYSSGETYESFSDQASARKSEMHYIVDTEGFYSSMHFDSGLKGSRSYYNYAAIDPDCGPSGNIAPGMTEYPQPEYRATRTLGRPCLSLRKPKAKPPPPKRSSSLKETSSDVNVPEQNEPKITCELPLPLSSREMKLQLDLADSAGHLETAGLESLGAWGVENVRDMLDCSSPFSSSDTHSFKDEGAVQADYADLWLHNDLKSNDPYRSLSNSSSATGTTVIECIKSPERSETHTCQPRSRPSSPTLPPPESEFKLASPEKLAGLASPSSGYSSQSETPTSSFPSAFFPGPLSPTSGKRKPKVPERKSSLCSLQQQQLSVRDPGISCRRETDFYAIPPSHLDLSALHSKHFPHRNQMHILHQNKQKAAIAAAAAAEARSAAAAAAEARSAAAAAAEARSAATAAAAAEACTAATASTKESATNTAPISAHLAITPTVLRSVQLRSICRPSDGNQGLDQDSSNLITRPKCPTIITDAPSSSNRHNRKPPAYKPPAAQVCDSQIPLVENIVFPQEEVRVRQERFGPGTYWAMTAFRTPVDPNCEKEVSLKRSSQCFQQEQDSRRCLDVQKTLSPERLKQDLNQLSRPDPSTQSMCLASTMPPPAYSEIQRSNFVLCNSPDKVPVSTQEASHSYTISDVQGREEDYETSGVATRSASQDIREEESTPDTEDYFSKDSTPSDNSSSPLTDDSRLDDDVFPSPNKLRTTEDLFAMIHRSKRKVLGRKDSGEVSGKGRPVVPPVTTPVSNPTVCPVIPAPPVPSNNSQRASGPIYRSAKKSSTSSEEFKLLLLKKGSRTDSSYRMSATEILKSPITPKTQAELLLEAMRQPEEIPLPQLDSTSSGDPLPSPFPKANSEGFSPKTLTMSAASRQGRSRIPPAANSSRYSTRSRLYTAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_021479860.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479860.1

ORF Insert Sequence:

ATGGCTTTTG CCTGCTGTGT TCCTGTCAGA TCGAGAGCGG TCTCGAATCT GGACATTGAG 
AGTAAACTGA CGTCGCACTA TCAGGCTCCA TGGCATCAGC AACACAATCT GTTCCACACT 
TGCACACGGC CAGCGTGTCT GGAGGAGCTG CACCGACATG CCAAGCTCAA CCTGCGGGCG 
CTGCACAGAG ACCAGCAGCA GCGTCAGCGT TCAACCAGCA GAGAGCGCAG TCGGGTCACT 
ATATCAATAT CTGTGGCACC GCCAATGCCA ACCTTCCCTT CAACTCACAA ACTCAGACGG 
CGAGAGCAGA GAGGTCGACA TTCACGCATG GAGAGGAGCG TTAGAGAGAG TGATGTGCAA 
ACTATCCAGA GGAAGGAGAG ACCAGCCAAG GATTCTGAAT TCAAACCTGT CCTCAGAAAG 
TATCAGCGCT CCCGCTCCCC CTCCCCTGTT CAGTGTTGTT ACTTCATTCC CTGGATTAGA 
AAGGCTGGAA CCAACACAGA AACGGATGGA GAACTACAAG TGATGAGCCA TAGGCCTAAA 
TGTCCTGTTC CCAATGCACC TACCACCCTG GACAAACAGA CTAACTGGTC TAAAGCCCTG 
CCTCTTCCCA CTCCAGAAGA AAGAATAAAG AATGACTCTC AAGTGATTTC ATCCTGTATC 
ATTCCAATAA ATGTTTCGGG TGTTGGATTT GACAGAGAAG CCAGTGCTCG CTGCTCTCTT 
GTTCACTCGC AATCGGTTCT ACAGAGACGG CGGAAACTCA GGAGGCGAAA AACGATCACT 
GGGATCCCCA AACGAGTTCA ACAAGACATG GATTCTGACG AATCCCCTGT TGCTAGAGAA 
AGAACAGTGA TAATCCACGC CAATCCACAC AAGTCCCACG AGTGGCATGA GGAGCTCTCC 
TTGAGTGGCA GAGTATTGCA TACTAAGGAT TCGGGCTGTC AGACAGATGA CTTCTTGATT 
ACTGCTCCAT CCCGAAGACG CATCCGTGCC CAAAGAGGTC AGGGAATTCC AGCGTCTCTC 
TCTCACTCCA CTGGAAACAT TACCTCCCTC CCGGATCGTT CTGATACGGT TTATGCTGCA 
GCATCAGCCA CTCGTGTCCG TTCTAGGAGC CTTCCACGTG AGGGTGGTCG GCTTCTGGAT 
GAGGACCAAG ATGACAGTGA TGATGATGAT GATGAAGATG ATGATGAAGA TGATGAAGAG 
GAAGATGAGG AGCTCTCTCC ATATGAAGCC GAAGACTTCC TGCCAGGTCC CGGACAGAGA 
ATTCCAAAGG ATGAGGAAGA GAGTACTGAC GACCAAGCCA TGCCAGAGCT ACAGTTTGGA 
AGTCTTAAGC GTATGCAGCA TTCAGAGAGC CCTGACCACA CATGGATTGA GAGAGGCAGA 
TCCAGACTCC CACGGAAAGT AGACATGGGG AGCTGTGAGA TTTCCTCTAG TTCAGATACT 
TTCAGCAGCC CCATACACTC TGCATCCACT ACAGGAGTGC TTGGCAGCCA GATTGACCAT 
AAAGAGGATC ACCAGTCCTC AAGTGGCAAC TGGAGTGGAA GCAGCTCCAC TTGCCCATCG 
CAGACATCCG AGACTCTTCC ACCTGCTGCC TCCCCTCCCT TGACGGGATC CTCACACTGT 
GATTCAGAAC TTTCCCTGAA TACTGGGTCC CATGTCATAG ATGACAACAC TGGTTTCATA 
ATTGATCCCT ATCACATAGA CAAGCCACAG GGACAGGGGC AACGATCCAA TTCATTTACC 
TCATCAGCTA CTGATCAGAT GGATGATGCA GGGGTCAGCA CTGCTAGTGA TGGGGAGTGG 
AACTGTGCCC AGGACCAGCA GGATCAGTCC TGCACCCAAG ACTTCAGCCC AAAGCGTTCC 
AGAGAGGATG AGAGTGGTGT CAGCTGCCCT AGTTATTCTA GTGGGGAAAC TTACGAGAGC 
TTCAGTGACC AAGCATCCGC TAGAAAATCT GAGATGCACT ACATTGTCGA CACTGAAGGA 
TTCTATTCCT CCATGCACTT TGACTCTGGT CTTAAGGGTA GCAGAAGCTA CTACAACTAT 
GCAGCCATTG ACCCCGACTG TGGCCCAAGT GGTAATATAG CACCTGGCAT GACTGAATAC 
CCTCAGCCAG AGTACAGAGC CACCAGGACA CTGGGCAGAC CCTGTCTCTC CTTAAGAAAA 
CCAAAGGCCA AGCCACCCCC ACCAAAACGT AGCTCTTCAT TAAAGGAAAC AAGCAGTGAT 
GTGAACGTTC CAGAGCAGAA CGAACCAAAG ATTACTTGTG AGTTGCCACT GCCCTTGTCT 
TCCAGGGAGA TGAAACTGCA GCTGGACTTG GCTGATTCTG CAGGGCACCT TGAGACTGCA 
GGTCTTGAAT CACTTGGTGC ATGGGGAGTG GAAAATGTTA GGGACATGCT TGACTGCTCC 
TCCCCATTCA GTTCCTCAGA CACACATTCC TTCAAAGATG AAGGTGCTGT GCAAGCAGAC 
TATGCAGACC TCTGGCTTCA CAATGACTTG AAATCAAATG ATCCTTATCG GTCCCTCTCT 
AACTCTAGCT CTGCTACAGG TACAACTGTT ATTGAATGCA TCAAGTCACC AGAAAGATCA 
GAAACGCACA CCTGTCAACC CAGGTCCAGA CCTTCTTCCC CAACTCTTCC TCCACCTGAA 
AGTGAATTTA AGCTTGCTTC TCCCGAAAAG CTGGCAGGCT TGGCGTCCCC TTCCAGTGGA 
TATTCCAGTC AGTCTGAAAC ACCTACATCT TCCTTCCCCT CTGCTTTCTT CCCAGGGCCC 
CTGTCTCCAA CCAGTGGGAA GAGGAAGCCC AAAGTCCCTG AAAGGAAGTC CTCACTTTGT 
TCCTTACAGC AGCAACAGCT TTCAGTCAGA GACCCAGGCA TTTCCTGTAG GAGAGAAACT 
GACTTCTATG CCATACCCCC AAGTCACCTT GACCTAAGTG CTCTTCACAG TAAGCACTTT 
CCTCACAGAA ATCAAATGCA CATCCTCCAC CAGAATAAGC AGAAAGCTGC CATAGCAGCA 
GCTGCTGCAG CAGAAGCTCG CAGTGCAGCA GCTGCTGCAG CAGAAGCTCG CAGTGCAGCA 
GCTGCTGCAG CAGAAGCTCG CAGTGCAGCA ACTGCAGCCG CTGCTGCCGA GGCCTGCACT 
GCAGCTACAG CTTCTACTAA AGAGAGTGCA ACAAATACAG CACCCATCTC AGCCCATTTG 
GCTATTACTC CAACGGTTCT TAGATCAGTG CAACTGCGAT CTATATGTAG ACCATCTGAT 
GGCAACCAAG GGCTTGATCA AGACAGTTCA AATCTCATAA CTCGTCCTAA GTGTCCCACA 
ATAATAACTG ATGCCCCATC ATCTAGCAAC AGGCACAACA GGAAGCCACC AGCCTACAAA 
CCCCCTGCTG CACAGGTTTG TGATTCACAG ATTCCACTGG TGGAAAACAT TGTTTTTCCA 
CAAGAAGAAG TGAGAGTGAG ACAGGAGAGG TTTGGTCCTG GTACTTACTG GGCAATGACT 
GCTTTCAGAA CTCCTGTGGA CCCTAATTGT GAAAAAGAAG TCTCTTTAAA AAGGTCATCT 
CAGTGTTTTC AGCAAGAACA AGACAGCAGA AGGTGCCTAG ATGTGCAAAA GACATTGTCA 
CCAGAGAGAC TAAAACAAGA TTTGAATCAA CTATCGCGGC CAGACCCTTC AACACAGTCC 
ATGTGTTTGG CCAGCACAAT GCCACCCCCT GCTTATAGTG AGATACAGAG GAGCAATTTT 
GTACTGTGCA ACAGTCCTGA CAAAGTGCCT GTTTCAACTC AAGAGGCATC GCATTCTTAC 
ACAATCAGCG ATGTACAAGG CAGAGAGGAG GATTATGAGA CATCAGGTGT CGCAACCAGA 
AGTGCCTCAC AGGACATAAG GGAAGAAGAG TCAACCCCAG ATACAGAGGA CTATTTCAGT 
AAAGACTCTA CTCCCAGTGA TAATTCATCC TCTCCTTTGA CCGATGACTC CAGACTTGAT 
GATGATGTTT TCCCATCTCC CAATAAACTC CGCACAACTG AAGATCTTTT TGCTATGATA 
CACAGATCTA AAAGGAAAGT GCTGGGACGC AAAGATTCAG GGGAAGTCAG TGGAAAAGGT 
CGGCCTGTTG TACCACCTGT GACCACCCCT GTAAGCAATC CTACTGTATG CCCGGTCATC 
CCTGCTCCCC CAGTTCCTTC AAACAACTCC CAGCGAGCCT CAGGACCCAT CTACAGGAGT 
GCCAAAAAGT CTAGTACATC CAGTGAGGAG TTCAAACTCC TTCTGCTTAA GAAGGGTAGT 
CGCACAGACT CAAGTTATCG CATGTCTGCT ACAGAGATCC TTAAGAGCCC CATCACACCC 
AAGACTCAAG CAGAGCTGCT GTTAGAGGCC ATGAGGCAAC CAGAGGAGAT CCCCTTACCC 
CAACTGGATT CCACCAGCAG TGGAGATCCA CTTCCAAGTC CATTCCCTAA GGCCAACAGT 
GAGGGATTCT CCCCAAAAAC ACTCACCATG TCAGCTGCCT CCAGACAAGG ACGTTCTAGA 
ATTCCACCTG CAGCGAACAG CAGTCGCTAT AGCACGCGGA GTCGGCTGTA CACTGCCCCA 
ATGCAGGCCA TATCAGAAGG AGAGACTGAG AACTCGGATG GAAGTCCACA CGATGACCGC 
TCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsb ( XM_009306118.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304393.1 Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X2, mRNA.

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CloneID	ODa43148
Clone ID Related Accession (Same CDS sequence)	XM_009306118.3 , XM_009306118.2
Accession Version	XM_009306118.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Nance-Horan syndrome protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306118.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA 
GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT 
CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA 
CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC 
AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC 
CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG 
AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT 
GAATTCAAAC CTGTCCTCAG AAAGCTGCAT TTCTTATCAA CACAGAAAAG TGGAAATTTG 
GCAATACATG CTGGAACCAA CACAGAAACG GATGGAGAAC TACAAGTGAT GAGCCATAGG 
CCTAAATGTC CTGTTCCCAA TGCACCTACC ACCCTGGACA AACAGACTAA CTGGTCTAAA 
GCCCTGCCTC TTCCCACTCC AGAAGAAAGA ATAAAGAATG ACTCTCAAGT GATTTCATCC 
TGTATCATTC CAATAAATGT TTCGGGTGTT GGATTTGACA GAGAAGCCAG TGCTCGCTGC 
TCTCTTGTTC ACTCGCAATC GGTTCTACAG AGACGGCGGA AACTCAGGAG GCGAAAAACG 
ATCACTGGGA TCCCCAAACG AGTTCAACAA GACATGGATT CTGACGAATC CCCTGTTGCT 
AGAGAAAGAA CAGTGATAAT CCACGCCAAT CCACACAAGT CCCACGAGTG GCATGAGGAG 
CTCTCCTTGA GTGGCAGAGT ATTGCATACT AAGGATTCGG GCTGTCAGAC AGATGACTTC 
TTGATTACTG CTCCATCCCG AAGACGCATC CGTGCCCAAA GAGGTCAGGG AATTCCAGCG 
TCTCTCTCTC ACTCCACTGG AAACATTACC TCCCTCCCGG ATCGTTCTGA TACGGTTTAT 
GCTGCAGCAT CAGCCACTCG TGTCCGTTCT AGGAGCCTTC CACGTGAGGG TGGTCGGCTT 
CTGGATGAGG ACCAAGATGA CAGTGATGAT GATGATGATG AAGATGATGA TGAAGATGAT 
GAAGAGGAAG ATGAGGAGCT CTCTCCATAT GAAGCCGAAG ACTTCCTGCC AGGTCCCGGA 
CAGAGAATTC CAAAGGATGA GGAAGAGAGT ACTGACGACC AAGCCATGCC AGAGCTACAG 
TTTGGAAGTC TTAAGCGTAT GCAGCATTCA GAGAGCCCTG ACCACACATG GATTGAGAGA 
GGCAGATCCA GACTCCCACG GAAAGTAGAC ATGGGGAGCT GTGAGATTTC CTCTAGTTCA 
GATACTTTCA GCAGCCCCAT ACACTCTGCA TCCACTACAG GAGTGCTTGG CAGCCAGATT 
GACCATAAAG AGGATCACCA GTCCTCAAGT GGCAACTGGA GTGGAAGCAG CTCCACTTGC 
CCATCGCAGA CATCCGAGAC TCTTCCACCT GCTGCCTCCC CTCCCTTGAC GGGATCCTCA 
CACTGTGATT CAGAACTTTC CCTGAATACT GGGTCCCATG TCATAGATGA CAACACTGGT 
TTCATAATTG ATCCCTATCA CATAGACAAG CCACAGGGAC AGGGGCAACG ATCCAATTCA 
TTTACCTCAT CAGCTACTGA TCAGATGGAT GATGCAGGGG TCAGCACTGC TAGTGATGGG 
GAGTGGAACT GTGCCCAGGA CCAGCAGGAT CAGTCCTGCA CCCAAGACTT CAGCCCAAAG 
CGTTCCAGAG AGGATGAGAG TGGTGTCAGC TGCCCTAGTT ATTCTAGTGG GGAAACTTAC 
GAGAGCTTCA GTGACCAAGC ATCCGCTAGA AAATCTGAGA TGCACTACAT TGTCGACACT 
GAAGGATTCT ATTCCTCCAT GCACTTTGAC TCTGGTCTTA AGGGTAGCAG AAGCTACTAC 
AACTATGCAG CCATTGACCC CGACTGTGGC CCAAGTGGTA ATATAGCACC TGGCATGACT 
GAATACCCTC AGCCAGAGTA CAGAGCCACC AGGACACTGG GCAGACCCTG TCTCTCCTTA 
AGAAAACCAA AGGCCAAGCC ACCCCCACCA AAACGTAGCT CTTCATTAAA GGAAACAAGC 
AGTGATGTGA ACGTTCCAGA GCAGAACGAA CCAAAGATTA CTTGTGAGTT GCCACTGCCC 
TTGTCTTCCA GGGAGATGAA ACTGCAGCTG GACTTGGCTG ATTCTGCAGG GCACCTTGAG 
ACTGCAGGTC TTGAATCACT TGGTGCATGG GGAGTGGAAA ATGTTAGGGA CATGCTTGAC 
TGCTCCTCCC CATTCAGTTC CTCAGACACA CATTCCTTCA AAGATGAAGG TGCTGTGCAA 
GCAGACTATG CAGACCTCTG GCTTCACAAT GACTTGAAAT CAAATGATCC TTATCGGTCC 
CTCTCTAACT CTAGCTCTGC TACAGGTACA ACTGTTATTG AATGCATCAA GTCACCAGAA 
AGATCAGAAA CGCACACCTG TCAACCCAGG TCCAGACCTT CTTCCCCAAC TCTTCCTCCA 
CCTGAAAGTG AATTTAAGCT TGCTTCTCCC GAAAAGCTGG CAGGCTTGGC GTCCCCTTCC 
AGTGGATATT CCAGTCAGTC TGAAACACCT ACATCTTCCT TCCCCTCTGC TTTCTTCCCA 
GGGCCCCTGT CTCCAACCAG TGGGAAGAGG AAGCCCAAAG TCCCTGAAAG GAAGTCCTCA 
CTTTGTTCCT TACAGCAGCA ACAGCTTTCA GTCAGAGACC CAGGCATTTC CTGTAGGAGA 
GAAACTGACT TCTATGCCAT ACCCCCAAGT CACCTTGACC TAAGTGCTCT TCACAGTAAG 
CACTTTCCTC ACAGAAATCA AATGCACATC CTCCACCAGA ATAAGCAGAA AGCTGCCATA 
GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT 
GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAACTG CAGCCGCTGC TGCCGAGGCC 
TGCACTGCAG CTACAGCTTC TACTAAAGAG AGTGCAACAA ATACAGCACC CATCTCAGCC 
CATTTGGCTA TTACTCCAAC GGTTCTTAGA TCAGTGCAAC TGCGATCTAT ATGTAGACCA 
TCTGATGGCA ACCAAGGGCT TGATCAAGAC AGTTCAAATC TCATAACTCG TCCTAAGTGT 
CCCACAATAA TAACTGATGC CCCATCATCT AGCAACAGGC ACAACAGGAA GCCACCAGCC 
TACAAACCCC CTGCTGCACA GGTTTGTGAT TCACAGATTC CACTGGTGGA AAACATTGTT 
TTTCCACAAG AAGAAGTGAG AGTGAGACAG GAGAGGTTTG GTCCTGGTAC TTACTGGGCA 
ATGACTGCTT TCAGAACTCC TGTGGACCCT AATTGTGAAA AAGAAGTCTC TTTAAAAAGG 
TCATCTCAGT GTTTTCAGCA AGAACAAGAC AGCAGAAGGT GCCTAGATGT GCAAAAGACA 
TTGTCACCAG AGAGACTAAA ACAAGATTTG AATCAACTAT CGCGGCCAGA CCCTTCAACA 
CAGTCCATGT GTTTGGCCAG CACAATGCCA CCCCCTGCTT ATAGTGAGAT ACAGAGGAGC 
AATTTTGTAC TGTGCAACAG TCCTGACAAA GTGCCTGTTT CAACTCAAGA GGCATCGCAT 
TCTTACACAA TCAGCGATGT ACAAGGCAGA GAGGAGGATT ATGAGACATC AGGTGTCGCA 
ACCAGAAGTG CCTCACAGGA CATAAGGGAA GAAGAGTCAA CCCCAGATAC AGAGGACTAT 
TTCAGTAAAG ACTCTACTCC CAGTGATAAT TCATCCTCTC CTTTGACCGA TGACTCCAGA 
CTTGATGATG ATGTTTTCCC ATCTCCCAAT AAACTCCGCA CAACTGAAGA TCTTTTTGCT 
ATGATACACA GATCTAAAAG GAAAGTGCTG GGACGCAAAG ATTCAGGGGA AGTCAGTGGA 
AAAGGTCGGC CTGTTGTACC ACCTGTGACC ACCCCTGTAA GCAATCCTAC TGTATGCCCG 
GTCATCCCTG CTCCCCCAGT TCCTTCAAAC AACTCCCAGC GAGCCTCAGG ACCCATCTAC 
AGGAGTGCCA AAAAGTCTAG TACATCCAGT GAGGAGTTCA AACTCCTTCT GCTTAAGAAG 
GGTAGTCGCA CAGACTCAAG TTATCGCATG TCTGCTACAG AGATCCTTAA GAGCCCCATC 
ACACCCAAGA CTCAAGCAGA GCTGCTGTTA GAGGCCATGA GGCAACCAGA GGAGATCCCC 
TTACCCCAAC TGGATTCCAC CAGCAGTGGA GATCCACTTC CAAGTCCATT CCCTAAGGCC 
AACAGTGAGG GATTCTCCCC AAAAACACTC ACCATGTCAG CTGCCTCCAG ACAAGGACGT 
TCTAGAATTC CACCTGCAGC GAACAGCAGT CGCTATAGCA CGCGGAGTCG GCTGTACACT 
GCCCCAATGC AGGCCATATC AGAAGGAGAG ACTGAGAACT CGGATGGAAG TCCACACGAT 
GACCGCTCCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304393.1
CDS	321..5195
Translation	MPFAKRIVEPQLLVRHPIPNDEGLLFEDLCSITNVALSRTLRQLSDLSKHACSLFQELENEIVSTNQRVWALQNKIGKIQQTASALDPKMEAVRFRSISIAVSNLDIESKLTSHYQAPWHQQHNLFHTCTRPACLEELHRHAKLNLRALHRDQQQRQRSTSRERSRVTISISVAPPMPTFPSTHKLRRREQRGRHSRMERSVRESDVQTIQRKERPAKDSEFKPVLRKLHFLSTQKSGNLAIHAGTNTETDGELQVMSHRPKCPVPNAPTTLDKQTNWSKALPLPTPEERIKNDSQVISSCIIPINVSGVGFDREASARCSLVHSQSVLQRRRKLRRRKTITGIPKRVQQDMDSDESPVARERTVIIHANPHKSHEWHEELSLSGRVLHTKDSGCQTDDFLITAPSRRRIRAQRGQGIPASLSHSTGNITSLPDRSDTVYAAASATRVRSRSLPREGGRLLDEDQDDSDDDDDEDDDEDDEEEDEELSPYEAEDFLPGPGQRIPKDEEESTDDQAMPELQFGSLKRMQHSESPDHTWIERGRSRLPRKVDMGSCEISSSSDTFSSPIHSASTTGVLGSQIDHKEDHQSSSGNWSGSSSTCPSQTSETLPPAASPPLTGSSHCDSELSLNTGSHVIDDNTGFIIDPYHIDKPQGQGQRSNSFTSSATDQMDDAGVSTASDGEWNCAQDQQDQSCTQDFSPKRSREDESGVSCPSYSSGETYESFSDQASARKSEMHYIVDTEGFYSSMHFDSGLKGSRSYYNYAAIDPDCGPSGNIAPGMTEYPQPEYRATRTLGRPCLSLRKPKAKPPPPKRSSSLKETSSDVNVPEQNEPKITCELPLPLSSREMKLQLDLADSAGHLETAGLESLGAWGVENVRDMLDCSSPFSSSDTHSFKDEGAVQADYADLWLHNDLKSNDPYRSLSNSSSATGTTVIECIKSPERSETHTCQPRSRPSSPTLPPPESEFKLASPEKLAGLASPSSGYSSQSETPTSSFPSAFFPGPLSPTSGKRKPKVPERKSSLCSLQQQQLSVRDPGISCRRETDFYAIPPSHLDLSALHSKHFPHRNQMHILHQNKQKAAIAAAAAAEARSAAAAAAEARSAAAAAAEARSAATAAAAAEACTAATASTKESATNTAPISAHLAITPTVLRSVQLRSICRPSDGNQGLDQDSSNLITRPKCPTIITDAPSSSNRHNRKPPAYKPPAAQVCDSQIPLVENIVFPQEEVRVRQERFGPGTYWAMTAFRTPVDPNCEKEVSLKRSSQCFQQEQDSRRCLDVQKTLSPERLKQDLNQLSRPDPSTQSMCLASTMPPPAYSEIQRSNFVLCNSPDKVPVSTQEASHSYTISDVQGREEDYETSGVATRSASQDIREEESTPDTEDYFSKDSTPSDNSSSPLTDDSRLDDDVFPSPNKLRTTEDLFAMIHRSKRKVLGRKDSGEVSGKGRPVVPPVTTPVSNPTVCPVIPAPPVPSNNSQRASGPIYRSAKKSSTSSEEFKLLLLKKGSRTDSSYRMSATEILKSPITPKTQAELLLEAMRQPEEIPLPQLDSTSSGDPLPSPFPKANSEGFSPKTLTMSAASRQGRSRIPPAANSSRYSTRSRLYTAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_009306118.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306118.3

ORF Insert Sequence:

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA 
GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT 
CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA 
CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC 
AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC 
CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG 
AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT 
GAATTCAAAC CTGTCCTCAG AAAGCTGCAT TTCTTATCAA CACAGAAAAG TGGAAATTTG 
GCAATACATG CTGGAACCAA CACAGAAACG GATGGAGAAC TACAAGTGAT GAGCCATAGG 
CCTAAATGTC CTGTTCCCAA TGCACCTACC ACCCTGGACA AACAGACTAA CTGGTCTAAA 
GCCCTGCCTC TTCCCACTCC AGAAGAAAGA ATAAAGAATG ACTCTCAAGT GATTTCATCC 
TGTATCATTC CAATAAATGT TTCGGGTGTT GGATTTGACA GAGAAGCCAG TGCTCGCTGC 
TCTCTTGTTC ACTCGCAATC GGTTCTACAG AGACGGCGGA AACTCAGGAG GCGAAAAACG 
ATCACTGGGA TCCCCAAACG AGTTCAACAA GACATGGATT CTGACGAATC CCCTGTTGCT 
AGAGAAAGAA CAGTGATAAT CCACGCCAAT CCACACAAGT CCCACGAGTG GCATGAGGAG 
CTCTCCTTGA GTGGCAGAGT ATTGCATACT AAGGATTCGG GCTGTCAGAC AGATGACTTC 
TTGATTACTG CTCCATCCCG AAGACGCATC CGTGCCCAAA GAGGTCAGGG AATTCCAGCG 
TCTCTCTCTC ACTCCACTGG AAACATTACC TCCCTCCCGG ATCGTTCTGA TACGGTTTAT 
GCTGCAGCAT CAGCCACTCG TGTCCGTTCT AGGAGCCTTC CACGTGAGGG TGGTCGGCTT 
CTGGATGAGG ACCAAGATGA CAGTGATGAT GATGATGATG AAGATGATGA TGAAGATGAT 
GAAGAGGAAG ATGAGGAGCT CTCTCCATAT GAAGCCGAAG ACTTCCTGCC AGGTCCCGGA 
CAGAGAATTC CAAAGGATGA GGAAGAGAGT ACTGACGACC AAGCCATGCC AGAGCTACAG 
TTTGGAAGTC TTAAGCGTAT GCAGCATTCA GAGAGCCCTG ACCACACATG GATTGAGAGA 
GGCAGATCCA GACTCCCACG GAAAGTAGAC ATGGGGAGCT GTGAGATTTC CTCTAGTTCA 
GATACTTTCA GCAGCCCCAT ACACTCTGCA TCCACTACAG GAGTGCTTGG CAGCCAGATT 
GACCATAAAG AGGATCACCA GTCCTCAAGT GGCAACTGGA GTGGAAGCAG CTCCACTTGC 
CCATCGCAGA CATCCGAGAC TCTTCCACCT GCTGCCTCCC CTCCCTTGAC GGGATCCTCA 
CACTGTGATT CAGAACTTTC CCTGAATACT GGGTCCCATG TCATAGATGA CAACACTGGT 
TTCATAATTG ATCCCTATCA CATAGACAAG CCACAGGGAC AGGGGCAACG ATCCAATTCA 
TTTACCTCAT CAGCTACTGA TCAGATGGAT GATGCAGGGG TCAGCACTGC TAGTGATGGG 
GAGTGGAACT GTGCCCAGGA CCAGCAGGAT CAGTCCTGCA CCCAAGACTT CAGCCCAAAG 
CGTTCCAGAG AGGATGAGAG TGGTGTCAGC TGCCCTAGTT ATTCTAGTGG GGAAACTTAC 
GAGAGCTTCA GTGACCAAGC ATCCGCTAGA AAATCTGAGA TGCACTACAT TGTCGACACT 
GAAGGATTCT ATTCCTCCAT GCACTTTGAC TCTGGTCTTA AGGGTAGCAG AAGCTACTAC 
AACTATGCAG CCATTGACCC CGACTGTGGC CCAAGTGGTA ATATAGCACC TGGCATGACT 
GAATACCCTC AGCCAGAGTA CAGAGCCACC AGGACACTGG GCAGACCCTG TCTCTCCTTA 
AGAAAACCAA AGGCCAAGCC ACCCCCACCA AAACGTAGCT CTTCATTAAA GGAAACAAGC 
AGTGATGTGA ACGTTCCAGA GCAGAACGAA CCAAAGATTA CTTGTGAGTT GCCACTGCCC 
TTGTCTTCCA GGGAGATGAA ACTGCAGCTG GACTTGGCTG ATTCTGCAGG GCACCTTGAG 
ACTGCAGGTC TTGAATCACT TGGTGCATGG GGAGTGGAAA ATGTTAGGGA CATGCTTGAC 
TGCTCCTCCC CATTCAGTTC CTCAGACACA CATTCCTTCA AAGATGAAGG TGCTGTGCAA 
GCAGACTATG CAGACCTCTG GCTTCACAAT GACTTGAAAT CAAATGATCC TTATCGGTCC 
CTCTCTAACT CTAGCTCTGC TACAGGTACA ACTGTTATTG AATGCATCAA GTCACCAGAA 
AGATCAGAAA CGCACACCTG TCAACCCAGG TCCAGACCTT CTTCCCCAAC TCTTCCTCCA 
CCTGAAAGTG AATTTAAGCT TGCTTCTCCC GAAAAGCTGG CAGGCTTGGC GTCCCCTTCC 
AGTGGATATT CCAGTCAGTC TGAAACACCT ACATCTTCCT TCCCCTCTGC TTTCTTCCCA 
GGGCCCCTGT CTCCAACCAG TGGGAAGAGG AAGCCCAAAG TCCCTGAAAG GAAGTCCTCA 
CTTTGTTCCT TACAGCAGCA ACAGCTTTCA GTCAGAGACC CAGGCATTTC CTGTAGGAGA 
GAAACTGACT TCTATGCCAT ACCCCCAAGT CACCTTGACC TAAGTGCTCT TCACAGTAAG 
CACTTTCCTC ACAGAAATCA AATGCACATC CTCCACCAGA ATAAGCAGAA AGCTGCCATA 
GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT 
GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAACTG CAGCCGCTGC TGCCGAGGCC 
TGCACTGCAG CTACAGCTTC TACTAAAGAG AGTGCAACAA ATACAGCACC CATCTCAGCC 
CATTTGGCTA TTACTCCAAC GGTTCTTAGA TCAGTGCAAC TGCGATCTAT ATGTAGACCA 
TCTGATGGCA ACCAAGGGCT TGATCAAGAC AGTTCAAATC TCATAACTCG TCCTAAGTGT 
CCCACAATAA TAACTGATGC CCCATCATCT AGCAACAGGC ACAACAGGAA GCCACCAGCC 
TACAAACCCC CTGCTGCACA GGTTTGTGAT TCACAGATTC CACTGGTGGA AAACATTGTT 
TTTCCACAAG AAGAAGTGAG AGTGAGACAG GAGAGGTTTG GTCCTGGTAC TTACTGGGCA 
ATGACTGCTT TCAGAACTCC TGTGGACCCT AATTGTGAAA AAGAAGTCTC TTTAAAAAGG 
TCATCTCAGT GTTTTCAGCA AGAACAAGAC AGCAGAAGGT GCCTAGATGT GCAAAAGACA 
TTGTCACCAG AGAGACTAAA ACAAGATTTG AATCAACTAT CGCGGCCAGA CCCTTCAACA 
CAGTCCATGT GTTTGGCCAG CACAATGCCA CCCCCTGCTT ATAGTGAGAT ACAGAGGAGC 
AATTTTGTAC TGTGCAACAG TCCTGACAAA GTGCCTGTTT CAACTCAAGA GGCATCGCAT 
TCTTACACAA TCAGCGATGT ACAAGGCAGA GAGGAGGATT ATGAGACATC AGGTGTCGCA 
ACCAGAAGTG CCTCACAGGA CATAAGGGAA GAAGAGTCAA CCCCAGATAC AGAGGACTAT 
TTCAGTAAAG ACTCTACTCC CAGTGATAAT TCATCCTCTC CTTTGACCGA TGACTCCAGA 
CTTGATGATG ATGTTTTCCC ATCTCCCAAT AAACTCCGCA CAACTGAAGA TCTTTTTGCT 
ATGATACACA GATCTAAAAG GAAAGTGCTG GGACGCAAAG ATTCAGGGGA AGTCAGTGGA 
AAAGGTCGGC CTGTTGTACC ACCTGTGACC ACCCCTGTAA GCAATCCTAC TGTATGCCCG 
GTCATCCCTG CTCCCCCAGT TCCTTCAAAC AACTCCCAGC GAGCCTCAGG ACCCATCTAC 
AGGAGTGCCA AAAAGTCTAG TACATCCAGT GAGGAGTTCA AACTCCTTCT GCTTAAGAAG 
GGTAGTCGCA CAGACTCAAG TTATCGCATG TCTGCTACAG AGATCCTTAA GAGCCCCATC 
ACACCCAAGA CTCAAGCAGA GCTGCTGTTA GAGGCCATGA GGCAACCAGA GGAGATCCCC 
TTACCCCAAC TGGATTCCAC CAGCAGTGGA GATCCACTTC CAAGTCCATT CCCTAAGGCC 
AACAGTGAGG GATTCTCCCC AAAAACACTC ACCATGTCAG CTGCCTCCAG ACAAGGACGT 
TCTAGAATTC CACCTGCAGC GAACAGCAGT CGCTATAGCA CGCGGAGTCG GCTGTACACT 
GCCCCAATGC AGGCCATATC AGAAGGAGAG ACTGAGAACT CGGATGGAAG TCCACACGAT 
GACCGCTCCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsb ( XM_009306118.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304393.1 Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X1, mRNA.

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CloneID	ODa43148
Clone ID Related Accession (Same CDS sequence)	XM_009306118.3 , XM_009306118.2
Accession Version	XM_009306118.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4875bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Nance-Horan syndrome protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306118.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA 
GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT 
CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA 
CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC 
AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC 
CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG 
AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT 
GAATTCAAAC CTGTCCTCAG AAAGCTGCAT TTCTTATCAA CACAGAAAAG TGGAAATTTG 
GCAATACATG CTGGAACCAA CACAGAAACG GATGGAGAAC TACAAGTGAT GAGCCATAGG 
CCTAAATGTC CTGTTCCCAA TGCACCTACC ACCCTGGACA AACAGACTAA CTGGTCTAAA 
GCCCTGCCTC TTCCCACTCC AGAAGAAAGA ATAAAGAATG ACTCTCAAGT GATTTCATCC 
TGTATCATTC CAATAAATGT TTCGGGTGTT GGATTTGACA GAGAAGCCAG TGCTCGCTGC 
TCTCTTGTTC ACTCGCAATC GGTTCTACAG AGACGGCGGA AACTCAGGAG GCGAAAAACG 
ATCACTGGGA TCCCCAAACG AGTTCAACAA GACATGGATT CTGACGAATC CCCTGTTGCT 
AGAGAAAGAA CAGTGATAAT CCACGCCAAT CCACACAAGT CCCACGAGTG GCATGAGGAG 
CTCTCCTTGA GTGGCAGAGT ATTGCATACT AAGGATTCGG GCTGTCAGAC AGATGACTTC 
TTGATTACTG CTCCATCCCG AAGACGCATC CGTGCCCAAA GAGGTCAGGG AATTCCAGCG 
TCTCTCTCTC ACTCCACTGG AAACATTACC TCCCTCCCGG ATCGTTCTGA TACGGTTTAT 
GCTGCAGCAT CAGCCACTCG TGTCCGTTCT AGGAGCCTTC CACGTGAGGG TGGTCGGCTT 
CTGGATGAGG ACCAAGATGA CAGTGATGAT GATGATGATG AAGATGATGA TGAAGATGAT 
GAAGAGGAAG ATGAGGAGCT CTCTCCATAT GAAGCCGAAG ACTTCCTGCC AGGTCCCGGA 
CAGAGAATTC CAAAGGATGA GGAAGAGAGT ACTGACGACC AAGCCATGCC AGAGCTACAG 
TTTGGAAGTC TTAAGCGTAT GCAGCATTCA GAGAGCCCTG ACCACACATG GATTGAGAGA 
GGCAGATCCA GACTCCCACG GAAAGTAGAC ATGGGGAGCT GTGAGATTTC CTCTAGTTCA 
GATACTTTCA GCAGCCCCAT ACACTCTGCA TCCACTACAG GAGTGCTTGG CAGCCAGATT 
GACCATAAAG AGGATCACCA GTCCTCAAGT GGCAACTGGA GTGGAAGCAG CTCCACTTGC 
CCATCGCAGA CATCCGAGAC TCTTCCACCT GCTGCCTCCC CTCCCTTGAC GGGATCCTCA 
CACTGTGATT CAGAACTTTC CCTGAATACT GGGTCCCATG TCATAGATGA CAACACTGGT 
TTCATAATTG ATCCCTATCA CATAGACAAG CCACAGGGAC AGGGGCAACG ATCCAATTCA 
TTTACCTCAT CAGCTACTGA TCAGATGGAT GATGCAGGGG TCAGCACTGC TAGTGATGGG 
GAGTGGAACT GTGCCCAGGA CCAGCAGGAT CAGTCCTGCA CCCAAGACTT CAGCCCAAAG 
CGTTCCAGAG AGGATGAGAG TGGTGTCAGC TGCCCTAGTT ATTCTAGTGG GGAAACTTAC 
GAGAGCTTCA GTGACCAAGC ATCCGCTAGA AAATCTGAGA TGCACTACAT TGTCGACACT 
GAAGGATTCT ATTCCTCCAT GCACTTTGAC TCTGGTCTTA AGGGTAGCAG AAGCTACTAC 
AACTATGCAG CCATTGACCC CGACTGTGGC CCAAGTGGTA ATATAGCACC TGGCATGACT 
GAATACCCTC AGCCAGAGTA CAGAGCCACC AGGACACTGG GCAGACCCTG TCTCTCCTTA 
AGAAAACCAA AGGCCAAGCC ACCCCCACCA AAACGTAGCT CTTCATTAAA GGAAACAAGC 
AGTGATGTGA ACGTTCCAGA GCAGAACGAA CCAAAGATTA CTTGTGAGTT GCCACTGCCC 
TTGTCTTCCA GGGAGATGAA ACTGCAGCTG GACTTGGCTG ATTCTGCAGG GCACCTTGAG 
ACTGCAGGTC TTGAATCACT TGGTGCATGG GGAGTGGAAA ATGTTAGGGA CATGCTTGAC 
TGCTCCTCCC CATTCAGTTC CTCAGACACA CATTCCTTCA AAGATGAAGG TGCTGTGCAA 
GCAGACTATG CAGACCTCTG GCTTCACAAT GACTTGAAAT CAAATGATCC TTATCGGTCC 
CTCTCTAACT CTAGCTCTGC TACAGGTACA ACTGTTATTG AATGCATCAA GTCACCAGAA 
AGATCAGAAA CGCACACCTG TCAACCCAGG TCCAGACCTT CTTCCCCAAC TCTTCCTCCA 
CCTGAAAGTG AATTTAAGCT TGCTTCTCCC GAAAAGCTGG CAGGCTTGGC GTCCCCTTCC 
AGTGGATATT CCAGTCAGTC TGAAACACCT ACATCTTCCT TCCCCTCTGC TTTCTTCCCA 
GGGCCCCTGT CTCCAACCAG TGGGAAGAGG AAGCCCAAAG TCCCTGAAAG GAAGTCCTCA 
CTTTGTTCCT TACAGCAGCA ACAGCTTTCA GTCAGAGACC CAGGCATTTC CTGTAGGAGA 
GAAACTGACT TCTATGCCAT ACCCCCAAGT CACCTTGACC TAAGTGCTCT TCACAGTAAG 
CACTTTCCTC ACAGAAATCA AATGCACATC CTCCACCAGA ATAAGCAGAA AGCTGCCATA 
GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT 
GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAACTG CAGCCGCTGC TGCCGAGGCC 
TGCACTGCAG CTACAGCTTC TACTAAAGAG AGTGCAACAA ATACAGCACC CATCTCAGCC 
CATTTGGCTA TTACTCCAAC GGTTCTTAGA TCAGTGCAAC TGCGATCTAT ATGTAGACCA 
TCTGATGGCA ACCAAGGGCT TGATCAAGAC AGTTCAAATC TCATAACTCG TCCTAAGTGT 
CCCACAATAA TAACTGATGC CCCATCATCT AGCAACAGGC ACAACAGGAA GCCACCAGCC 
TACAAACCCC CTGCTGCACA GGTTTGTGAT TCACAGATTC CACTGGTGGA AAACATTGTT 
TTTCCACAAG AAGAAGTGAG AGTGAGACAG GAGAGGTTTG GTCCTGGTAC TTACTGGGCA 
ATGACTGCTT TCAGAACTCC TGTGGACCCT AATTGTGAAA AAGAAGTCTC TTTAAAAAGG 
TCATCTCAGT GTTTTCAGCA AGAACAAGAC AGCAGAAGGT GCCTAGATGT GCAAAAGACA 
TTGTCACCAG AGAGACTAAA ACAAGATTTG AATCAACTAT CGCGGCCAGA CCCTTCAACA 
CAGTCCATGT GTTTGGCCAG CACAATGCCA CCCCCTGCTT ATAGTGAGAT ACAGAGGAGC 
AATTTTGTAC TGTGCAACAG TCCTGACAAA GTGCCTGTTT CAACTCAAGA GGCATCGCAT 
TCTTACACAA TCAGCGATGT ACAAGGCAGA GAGGAGGATT ATGAGACATC AGGTGTCGCA 
ACCAGAAGTG CCTCACAGGA CATAAGGGAA GAAGAGTCAA CCCCAGATAC AGAGGACTAT 
TTCAGTAAAG ACTCTACTCC CAGTGATAAT TCATCCTCTC CTTTGACCGA TGACTCCAGA 
CTTGATGATG ATGTTTTCCC ATCTCCCAAT AAACTCCGCA CAACTGAAGA TCTTTTTGCT 
ATGATACACA GATCTAAAAG GAAAGTGCTG GGACGCAAAG ATTCAGGGGA AGTCAGTGGA 
AAAGGTCGGC CTGTTGTACC ACCTGTGACC ACCCCTGTAA GCAATCCTAC TGTATGCCCG 
GTCATCCCTG CTCCCCCAGT TCCTTCAAAC AACTCCCAGC GAGCCTCAGG ACCCATCTAC 
AGGAGTGCCA AAAAGTCTAG TACATCCAGT GAGGAGTTCA AACTCCTTCT GCTTAAGAAG 
GGTAGTCGCA CAGACTCAAG TTATCGCATG TCTGCTACAG AGATCCTTAA GAGCCCCATC 
ACACCCAAGA CTCAAGCAGA GCTGCTGTTA GAGGCCATGA GGCAACCAGA GGAGATCCCC 
TTACCCCAAC TGGATTCCAC CAGCAGTGGA GATCCACTTC CAAGTCCATT CCCTAAGGCC 
AACAGTGAGG GATTCTCCCC AAAAACACTC ACCATGTCAG CTGCCTCCAG ACAAGGACGT 
TCTAGAATTC CACCTGCAGC GAACAGCAGT CGCTATAGCA CGCGGAGTCG GCTGTACACT 
GCCCCAATGC AGGCCATATC AGAAGGAGAG ACTGAGAACT CGGATGGAAG TCCACACGAT 
GACCGCTCCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304393.1
CDS	291..5165
Translation	MPFAKRIVEPQLLVRHPIPNDEGLLFEDLCSITNVALSRTLRQLSDLSKHACSLFQELENEIVSTNQRVWALQNKIGKIQQTASALDPKMEAVRFRSISIAVSNLDIESKLTSHYQAPWHQQHNLFHTCTRPACLEELHRHAKLNLRALHRDQQQRQRSTSRERSRVTISISVAPPMPTFPSTHKLRRREQRGRHSRMERSVRESDVQTIQRKERPAKDSEFKPVLRKLHFLSTQKSGNLAIHAGTNTETDGELQVMSHRPKCPVPNAPTTLDKQTNWSKALPLPTPEERIKNDSQVISSCIIPINVSGVGFDREASARCSLVHSQSVLQRRRKLRRRKTITGIPKRVQQDMDSDESPVARERTVIIHANPHKSHEWHEELSLSGRVLHTKDSGCQTDDFLITAPSRRRIRAQRGQGIPASLSHSTGNITSLPDRSDTVYAAASATRVRSRSLPREGGRLLDEDQDDSDDDDDEDDDEDDEEEDEELSPYEAEDFLPGPGQRIPKDEEESTDDQAMPELQFGSLKRMQHSESPDHTWIERGRSRLPRKVDMGSCEISSSSDTFSSPIHSASTTGVLGSQIDHKEDHQSSSGNWSGSSSTCPSQTSETLPPAASPPLTGSSHCDSELSLNTGSHVIDDNTGFIIDPYHIDKPQGQGQRSNSFTSSATDQMDDAGVSTASDGEWNCAQDQQDQSCTQDFSPKRSREDESGVSCPSYSSGETYESFSDQASARKSEMHYIVDTEGFYSSMHFDSGLKGSRSYYNYAAIDPDCGPSGNIAPGMTEYPQPEYRATRTLGRPCLSLRKPKAKPPPPKRSSSLKETSSDVNVPEQNEPKITCELPLPLSSREMKLQLDLADSAGHLETAGLESLGAWGVENVRDMLDCSSPFSSSDTHSFKDEGAVQADYADLWLHNDLKSNDPYRSLSNSSSATGTTVIECIKSPERSETHTCQPRSRPSSPTLPPPESEFKLASPEKLAGLASPSSGYSSQSETPTSSFPSAFFPGPLSPTSGKRKPKVPERKSSLCSLQQQQLSVRDPGISCRRETDFYAIPPSHLDLSALHSKHFPHRNQMHILHQNKQKAAIAAAAAAEARSAAAAAAEARSAAAAAAEARSAATAAAAAEACTAATASTKESATNTAPISAHLAITPTVLRSVQLRSICRPSDGNQGLDQDSSNLITRPKCPTIITDAPSSSNRHNRKPPAYKPPAAQVCDSQIPLVENIVFPQEEVRVRQERFGPGTYWAMTAFRTPVDPNCEKEVSLKRSSQCFQQEQDSRRCLDVQKTLSPERLKQDLNQLSRPDPSTQSMCLASTMPPPAYSEIQRSNFVLCNSPDKVPVSTQEASHSYTISDVQGREEDYETSGVATRSASQDIREEESTPDTEDYFSKDSTPSDNSSSPLTDDSRLDDDVFPSPNKLRTTEDLFAMIHRSKRKVLGRKDSGEVSGKGRPVVPPVTTPVSNPTVCPVIPAPPVPSNNSQRASGPIYRSAKKSSTSSEEFKLLLLKKGSRTDSSYRMSATEILKSPITPKTQAELLLEAMRQPEEIPLPQLDSTSSGDPLPSPFPKANSEGFSPKTLTMSAASRQGRSRIPPAANSSRYSTRSRLYTAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_009306118.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306118.2

ORF Insert Sequence:

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA 
GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT 
CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA 
CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC 
AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC 
CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG 
AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT 
GAATTCAAAC CTGTCCTCAG AAAGCTGCAT TTCTTATCAA CACAGAAAAG TGGAAATTTG 
GCAATACATG CTGGAACCAA CACAGAAACG GATGGAGAAC TACAAGTGAT GAGCCATAGG 
CCTAAATGTC CTGTTCCCAA TGCACCTACC ACCCTGGACA AACAGACTAA CTGGTCTAAA 
GCCCTGCCTC TTCCCACTCC AGAAGAAAGA ATAAAGAATG ACTCTCAAGT GATTTCATCC 
TGTATCATTC CAATAAATGT TTCGGGTGTT GGATTTGACA GAGAAGCCAG TGCTCGCTGC 
TCTCTTGTTC ACTCGCAATC GGTTCTACAG AGACGGCGGA AACTCAGGAG GCGAAAAACG 
ATCACTGGGA TCCCCAAACG AGTTCAACAA GACATGGATT CTGACGAATC CCCTGTTGCT 
AGAGAAAGAA CAGTGATAAT CCACGCCAAT CCACACAAGT CCCACGAGTG GCATGAGGAG 
CTCTCCTTGA GTGGCAGAGT ATTGCATACT AAGGATTCGG GCTGTCAGAC AGATGACTTC 
TTGATTACTG CTCCATCCCG AAGACGCATC CGTGCCCAAA GAGGTCAGGG AATTCCAGCG 
TCTCTCTCTC ACTCCACTGG AAACATTACC TCCCTCCCGG ATCGTTCTGA TACGGTTTAT 
GCTGCAGCAT CAGCCACTCG TGTCCGTTCT AGGAGCCTTC CACGTGAGGG TGGTCGGCTT 
CTGGATGAGG ACCAAGATGA CAGTGATGAT GATGATGATG AAGATGATGA TGAAGATGAT 
GAAGAGGAAG ATGAGGAGCT CTCTCCATAT GAAGCCGAAG ACTTCCTGCC AGGTCCCGGA 
CAGAGAATTC CAAAGGATGA GGAAGAGAGT ACTGACGACC AAGCCATGCC AGAGCTACAG 
TTTGGAAGTC TTAAGCGTAT GCAGCATTCA GAGAGCCCTG ACCACACATG GATTGAGAGA 
GGCAGATCCA GACTCCCACG GAAAGTAGAC ATGGGGAGCT GTGAGATTTC CTCTAGTTCA 
GATACTTTCA GCAGCCCCAT ACACTCTGCA TCCACTACAG GAGTGCTTGG CAGCCAGATT 
GACCATAAAG AGGATCACCA GTCCTCAAGT GGCAACTGGA GTGGAAGCAG CTCCACTTGC 
CCATCGCAGA CATCCGAGAC TCTTCCACCT GCTGCCTCCC CTCCCTTGAC GGGATCCTCA 
CACTGTGATT CAGAACTTTC CCTGAATACT GGGTCCCATG TCATAGATGA CAACACTGGT 
TTCATAATTG ATCCCTATCA CATAGACAAG CCACAGGGAC AGGGGCAACG ATCCAATTCA 
TTTACCTCAT CAGCTACTGA TCAGATGGAT GATGCAGGGG TCAGCACTGC TAGTGATGGG 
GAGTGGAACT GTGCCCAGGA CCAGCAGGAT CAGTCCTGCA CCCAAGACTT CAGCCCAAAG 
CGTTCCAGAG AGGATGAGAG TGGTGTCAGC TGCCCTAGTT ATTCTAGTGG GGAAACTTAC 
GAGAGCTTCA GTGACCAAGC ATCCGCTAGA AAATCTGAGA TGCACTACAT TGTCGACACT 
GAAGGATTCT ATTCCTCCAT GCACTTTGAC TCTGGTCTTA AGGGTAGCAG AAGCTACTAC 
AACTATGCAG CCATTGACCC CGACTGTGGC CCAAGTGGTA ATATAGCACC TGGCATGACT 
GAATACCCTC AGCCAGAGTA CAGAGCCACC AGGACACTGG GCAGACCCTG TCTCTCCTTA 
AGAAAACCAA AGGCCAAGCC ACCCCCACCA AAACGTAGCT CTTCATTAAA GGAAACAAGC 
AGTGATGTGA ACGTTCCAGA GCAGAACGAA CCAAAGATTA CTTGTGAGTT GCCACTGCCC 
TTGTCTTCCA GGGAGATGAA ACTGCAGCTG GACTTGGCTG ATTCTGCAGG GCACCTTGAG 
ACTGCAGGTC TTGAATCACT TGGTGCATGG GGAGTGGAAA ATGTTAGGGA CATGCTTGAC 
TGCTCCTCCC CATTCAGTTC CTCAGACACA CATTCCTTCA AAGATGAAGG TGCTGTGCAA 
GCAGACTATG CAGACCTCTG GCTTCACAAT GACTTGAAAT CAAATGATCC TTATCGGTCC 
CTCTCTAACT CTAGCTCTGC TACAGGTACA ACTGTTATTG AATGCATCAA GTCACCAGAA 
AGATCAGAAA CGCACACCTG TCAACCCAGG TCCAGACCTT CTTCCCCAAC TCTTCCTCCA 
CCTGAAAGTG AATTTAAGCT TGCTTCTCCC GAAAAGCTGG CAGGCTTGGC GTCCCCTTCC 
AGTGGATATT CCAGTCAGTC TGAAACACCT ACATCTTCCT TCCCCTCTGC TTTCTTCCCA 
GGGCCCCTGT CTCCAACCAG TGGGAAGAGG AAGCCCAAAG TCCCTGAAAG GAAGTCCTCA 
CTTTGTTCCT TACAGCAGCA ACAGCTTTCA GTCAGAGACC CAGGCATTTC CTGTAGGAGA 
GAAACTGACT TCTATGCCAT ACCCCCAAGT CACCTTGACC TAAGTGCTCT TCACAGTAAG 
CACTTTCCTC ACAGAAATCA AATGCACATC CTCCACCAGA ATAAGCAGAA AGCTGCCATA 
GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT 
GCAGCAGCTG CTGCAGCAGA AGCTCGCAGT GCAGCAACTG CAGCCGCTGC TGCCGAGGCC 
TGCACTGCAG CTACAGCTTC TACTAAAGAG AGTGCAACAA ATACAGCACC CATCTCAGCC 
CATTTGGCTA TTACTCCAAC GGTTCTTAGA TCAGTGCAAC TGCGATCTAT ATGTAGACCA 
TCTGATGGCA ACCAAGGGCT TGATCAAGAC AGTTCAAATC TCATAACTCG TCCTAAGTGT 
CCCACAATAA TAACTGATGC CCCATCATCT AGCAACAGGC ACAACAGGAA GCCACCAGCC 
TACAAACCCC CTGCTGCACA GGTTTGTGAT TCACAGATTC CACTGGTGGA AAACATTGTT 
TTTCCACAAG AAGAAGTGAG AGTGAGACAG GAGAGGTTTG GTCCTGGTAC TTACTGGGCA 
ATGACTGCTT TCAGAACTCC TGTGGACCCT AATTGTGAAA AAGAAGTCTC TTTAAAAAGG 
TCATCTCAGT GTTTTCAGCA AGAACAAGAC AGCAGAAGGT GCCTAGATGT GCAAAAGACA 
TTGTCACCAG AGAGACTAAA ACAAGATTTG AATCAACTAT CGCGGCCAGA CCCTTCAACA 
CAGTCCATGT GTTTGGCCAG CACAATGCCA CCCCCTGCTT ATAGTGAGAT ACAGAGGAGC 
AATTTTGTAC TGTGCAACAG TCCTGACAAA GTGCCTGTTT CAACTCAAGA GGCATCGCAT 
TCTTACACAA TCAGCGATGT ACAAGGCAGA GAGGAGGATT ATGAGACATC AGGTGTCGCA 
ACCAGAAGTG CCTCACAGGA CATAAGGGAA GAAGAGTCAA CCCCAGATAC AGAGGACTAT 
TTCAGTAAAG ACTCTACTCC CAGTGATAAT TCATCCTCTC CTTTGACCGA TGACTCCAGA 
CTTGATGATG ATGTTTTCCC ATCTCCCAAT AAACTCCGCA CAACTGAAGA TCTTTTTGCT 
ATGATACACA GATCTAAAAG GAAAGTGCTG GGACGCAAAG ATTCAGGGGA AGTCAGTGGA 
AAAGGTCGGC CTGTTGTACC ACCTGTGACC ACCCCTGTAA GCAATCCTAC TGTATGCCCG 
GTCATCCCTG CTCCCCCAGT TCCTTCAAAC AACTCCCAGC GAGCCTCAGG ACCCATCTAC 
AGGAGTGCCA AAAAGTCTAG TACATCCAGT GAGGAGTTCA AACTCCTTCT GCTTAAGAAG 
GGTAGTCGCA CAGACTCAAG TTATCGCATG TCTGCTACAG AGATCCTTAA GAGCCCCATC 
ACACCCAAGA CTCAAGCAGA GCTGCTGTTA GAGGCCATGA GGCAACCAGA GGAGATCCCC 
TTACCCCAAC TGGATTCCAC CAGCAGTGGA GATCCACTTC CAAGTCCATT CCCTAAGGCC 
AACAGTGAGG GATTCTCCCC AAAAACACTC ACCATGTCAG CTGCCTCCAG ACAAGGACGT 
TCTAGAATTC CACCTGCAGC GAACAGCAGT CGCTATAGCA CGCGGAGTCG GCTGTACACT 
GCCCCAATGC AGGCCATATC AGAAGGAGAG ACTGAGAACT CGGATGGAAG TCCACACGAT 
GACCGCTCCT CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsb ( XM_009306119.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304394.1 Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X2, mRNA.

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CloneID	ODa43149
Clone ID Related Accession (Same CDS sequence)	XM_009306119.2 , XM_009306119.3
Accession Version	XM_009306119.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Nance-Horan syndrome protein isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC CGGTCTCGAA TCTGGACATT 
GAGAGTAAAC TGACGTCGCA CTATCAGGCT CCATGGCATC AGCAACACAA TCTGTTCCAC 
ACTTGCACAC GGCCAGCGTG TCTGGAGGAG CTGCACCGAC ATGCCAAGCT CAACCTGCGG 
GCGCTGCACA GAGACCAGCA GCAGCGTCAG CGTTCAACCA GCAGAGAGCG CAGTCGGGTC 
ACTATATCAA TATCTGTGGC ACCGCCAATG CCAACCTTCC CTTCAACTCA CAAACTCAGA 
CGGCGAGAGC AGAGAGGTCG ACATTCACGC ATGGAGAGGA GCGTTAGAGA GAGTGATGTG 
CAAACTATCC AGAGGAAGGA GAGACCAGCC AAGGATTCTG AATTCAAACC TGTCCTCAGA 
AAGTATCAGC GCTCCCGCTC CCCCTCCCCT GTTCAGTGTT GTTACTTCAT TCCCTGGATT 
AGAAAGGCTG GAACCAACAC AGAAACGGAT GGAGAACTAC AAGTGATGAG CCATAGGCCT 
AAATGTCCTG TTCCCAATGC ACCTACCACC CTGGACAAAC AGACTAACTG GTCTAAAGCC 
CTGCCTCTTC CCACTCCAGA AGAAAGAATA AAGAATGACT CTCAAGTGAT TTCATCCTGT 
ATCATTCCAA TAAATGTTTC GGGTGTTGGA TTTGACAGAG AAGCCAGTGC TCGCTGCTCT 
CTTGTTCACT CGCAATCGGT TCTACAGAGA CGGCGGAAAC TCAGGAGGCG AAAAACGATC 
ACTGGGATCC CCAAACGAGT TCAACAAGAC ATGGATTCTG ACGAATCCCC TGTTGCTAGA 
GAAAGAACAG TGATAATCCA CGCCAATCCA CACAAGTCCC ACGAGTGGCA TGAGGAGCTC 
TCCTTGAGTG GCAGAGTATT GCATACTAAG GATTCGGGCT GTCAGACAGA TGACTTCTTG 
ATTACTGCTC CATCCCGAAG ACGCATCCGT GCCCAAAGAG GTCAGGGAAT TCCAGCGTCT 
CTCTCTCACT CCACTGGAAA CATTACCTCC CTCCCGGATC GTTCTGATAC GGTTTATGCT 
GCAGCATCAG CCACTCGTGT CCGTTCTAGG AGCCTTCCAC GTGAGGGTGG TCGGCTTCTG 
GATGAGGACC AAGATGACAG TGATGATGAT GATGATGAAG ATGATGATGA AGATGATGAA 
GAGGAAGATG AGGAGCTCTC TCCATATGAA GCCGAAGACT TCCTGCCAGG TCCCGGACAG 
AGAATTCCAA AGGATGAGGA AGAGAGTACT GACGACCAAG CCATGCCAGA GCTACAGTTT 
GGAAGTCTTA AGCGTATGCA GCATTCAGAG AGCCCTGACC ACACATGGAT TGAGAGAGGC 
AGATCCAGAC TCCCACGGAA AGTAGACATG GGGAGCTGTG AGATTTCCTC TAGTTCAGAT 
ACTTTCAGCA GCCCCATACA CTCTGCATCC ACTACAGGAG TGCTTGGCAG CCAGATTGAC 
CATAAAGAGG ATCACCAGTC CTCAAGTGGC AACTGGAGTG GAAGCAGCTC CACTTGCCCA 
TCGCAGACAT CCGAGACTCT TCCACCTGCT GCCTCCCCTC CCTTGACGGG ATCCTCACAC 
TGTGATTCAG AACTTTCCCT GAATACTGGG TCCCATGTCA TAGATGACAA CACTGGTTTC 
ATAATTGATC CCTATCACAT AGACAAGCCA CAGGGACAGG GGCAACGATC CAATTCATTT 
ACCTCATCAG CTACTGATCA GATGGATGAT GCAGGGGTCA GCACTGCTAG TGATGGGGAG 
TGGAACTGTG CCCAGGACCA GCAGGATCAG TCCTGCACCC AAGACTTCAG CCCAAAGCGT 
TCCAGAGAGG ATGAGAGTGG TGTCAGCTGC CCTAGTTATT CTAGTGGGGA AACTTACGAG 
AGCTTCAGTG ACCAAGCATC CGCTAGAAAA TCTGAGATGC ACTACATTGT CGACACTGAA 
GGATTCTATT CCTCCATGCA CTTTGACTCT GGTCTTAAGG GTAGCAGAAG CTACTACAAC 
TATGCAGCCA TTGACCCCGA CTGTGGCCCA AGTGGTAATA TAGCACCTGG CATGACTGAA 
TACCCTCAGC CAGAGTACAG AGCCACCAGG ACACTGGGCA GACCCTGTCT CTCCTTAAGA 
AAACCAAAGG CCAAGCCACC CCCACCAAAA CGTAGCTCTT CATTAAAGGA AACAAGCAGT 
GATGTGAACG TTCCAGAGCA GAACGAACCA AAGATTACTT GTGAGTTGCC ACTGCCCTTG 
TCTTCCAGGG AGATGAAACT GCAGCTGGAC TTGGCTGATT CTGCAGGGCA CCTTGAGACT 
GCAGGTCTTG AATCACTTGG TGCATGGGGA GTGGAAAATG TTAGGGACAT GCTTGACTGC 
TCCTCCCCAT TCAGTTCCTC AGACACACAT TCCTTCAAAG ATGAAGGTGC TGTGCAAGCA 
GACTATGCAG ACCTCTGGCT TCACAATGAC TTGAAATCAA ATGATCCTTA TCGGTCCCTC 
TCTAACTCTA GCTCTGCTAC AGGTACAACT GTTATTGAAT GCATCAAGTC ACCAGAAAGA 
TCAGAAACGC ACACCTGTCA ACCCAGGTCC AGACCTTCTT CCCCAACTCT TCCTCCACCT 
GAAAGTGAAT TTAAGCTTGC TTCTCCCGAA AAGCTGGCAG GCTTGGCGTC CCCTTCCAGT 
GGATATTCCA GTCAGTCTGA AACACCTACA TCTTCCTTCC CCTCTGCTTT CTTCCCAGGG 
CCCCTGTCTC CAACCAGTGG GAAGAGGAAG CCCAAAGTCC CTGAAAGGAA GTCCTCACTT 
TGTTCCTTAC AGCAGCAACA GCTTTCAGTC AGAGACCCAG GCATTTCCTG TAGGAGAGAA 
ACTGACTTCT ATGCCATACC CCCAAGTCAC CTTGACCTAA GTGCTCTTCA CAGTAAGCAC 
TTTCCTCACA GAAATCAAAT GCACATCCTC CACCAGAATA AGCAGAAAGC TGCCATAGCA 
GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA 
GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAACTGCAG CCGCTGCTGC CGAGGCCTGC 
ACTGCAGCTA CAGCTTCTAC TAAAGAGAGT GCAACAAATA CAGCACCCAT CTCAGCCCAT 
TTGGCTATTA CTCCAACGGT TCTTAGATCA GTGCAACTGC GATCTATATG TAGACCATCT 
GATGGCAACC AAGGGCTTGA TCAAGACAGT TCAAATCTCA TAACTCGTCC TAAGTGTCCC 
ACAATAATAA CTGATGCCCC ATCATCTAGC AACAGGCACA ACAGGAAGCC ACCAGCCTAC 
AAACCCCCTG CTGCACAGGT TTGTGATTCA CAGATTCCAC TGGTGGAAAA CATTGTTTTT 
CCACAAGAAG AAGTGAGAGT GAGACAGGAG AGGTTTGGTC CTGGTACTTA CTGGGCAATG 
ACTGCTTTCA GAACTCCTGT GGACCCTAAT TGTGAAAAAG AAGTCTCTTT AAAAAGGTCA 
TCTCAGTGTT TTCAGCAAGA ACAAGACAGC AGAAGGTGCC TAGATGTGCA AAAGACATTG 
TCACCAGAGA GACTAAAACA AGATTTGAAT CAACTATCGC GGCCAGACCC TTCAACACAG 
TCCATGTGTT TGGCCAGCAC AATGCCACCC CCTGCTTATA GTGAGATACA GAGGAGCAAT 
TTTGTACTGT GCAACAGTCC TGACAAAGTG CCTGTTTCAA CTCAAGAGGC ATCGCATTCT 
TACACAATCA GCGATGTACA AGGCAGAGAG GAGGATTATG AGACATCAGG TGTCGCAACC 
AGAAGTGCCT CACAGGACAT AAGGGAAGAA GAGTCAACCC CAGATACAGA GGACTATTTC 
AGTAAAGACT CTACTCCCAG TGATAATTCA TCCTCTCCTT TGACCGATGA CTCCAGACTT 
GATGATGATG TTTTCCCATC TCCCAATAAA CTCCGCACAA CTGAAGATCT TTTTGCTATG 
ATACACAGAT CTAAAAGGAA AGTGCTGGGA CGCAAAGATT CAGGGGAAGT CAGTGGAAAA 
GGTCGGCCTG TTGTACCACC TGTGACCACC CCTGTAAGCA ATCCTACTGT ATGCCCGGTC 
ATCCCTGCTC CCCCAGTTCC TTCAAACAAC TCCCAGCGAG CCTCAGGACC CATCTACAGG 
AGTGCCAAAA AGTCTAGTAC ATCCAGTGAG GAGTTCAAAC TCCTTCTGCT TAAGAAGGGT 
AGTCGCACAG ACTCAAGTTA TCGCATGTCT GCTACAGAGA TCCTTAAGAG CCCCATCACA 
CCCAAGACTC AAGCAGAGCT GCTGTTAGAG GCCATGAGGC AACCAGAGGA GATCCCCTTA 
CCCCAACTGG ATTCCACCAG CAGTGGAGAT CCACTTCCAA GTCCATTCCC TAAGGCCAAC 
AGTGAGGGAT TCTCCCCAAA AACACTCACC ATGTCAGCTG CCTCCAGACA AGGACGTTCT 
AGAATTCCAC CTGCAGCGAA CAGCAGTCGC TATAGCACGC GGAGTCGGCT GTACACTGCC 
CCAATGCAGG CCATATCAGA AGGAGAGACT GAGAACTCGG ATGGAAGTCC ACACGATGAC 
CGCTCCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304394.1
CDS	291..5162
Translation	MPFAKRIVEPQLLVRHPIPNDEGLLFEDLCSITNVALSRTLRQLSDLSKHACSLFQELENEIVSTNQRVWALQNKIGKIQQTASALDPKMEAVPVSNLDIESKLTSHYQAPWHQQHNLFHTCTRPACLEELHRHAKLNLRALHRDQQQRQRSTSRERSRVTISISVAPPMPTFPSTHKLRRREQRGRHSRMERSVRESDVQTIQRKERPAKDSEFKPVLRKYQRSRSPSPVQCCYFIPWIRKAGTNTETDGELQVMSHRPKCPVPNAPTTLDKQTNWSKALPLPTPEERIKNDSQVISSCIIPINVSGVGFDREASARCSLVHSQSVLQRRRKLRRRKTITGIPKRVQQDMDSDESPVARERTVIIHANPHKSHEWHEELSLSGRVLHTKDSGCQTDDFLITAPSRRRIRAQRGQGIPASLSHSTGNITSLPDRSDTVYAAASATRVRSRSLPREGGRLLDEDQDDSDDDDDEDDDEDDEEEDEELSPYEAEDFLPGPGQRIPKDEEESTDDQAMPELQFGSLKRMQHSESPDHTWIERGRSRLPRKVDMGSCEISSSSDTFSSPIHSASTTGVLGSQIDHKEDHQSSSGNWSGSSSTCPSQTSETLPPAASPPLTGSSHCDSELSLNTGSHVIDDNTGFIIDPYHIDKPQGQGQRSNSFTSSATDQMDDAGVSTASDGEWNCAQDQQDQSCTQDFSPKRSREDESGVSCPSYSSGETYESFSDQASARKSEMHYIVDTEGFYSSMHFDSGLKGSRSYYNYAAIDPDCGPSGNIAPGMTEYPQPEYRATRTLGRPCLSLRKPKAKPPPPKRSSSLKETSSDVNVPEQNEPKITCELPLPLSSREMKLQLDLADSAGHLETAGLESLGAWGVENVRDMLDCSSPFSSSDTHSFKDEGAVQADYADLWLHNDLKSNDPYRSLSNSSSATGTTVIECIKSPERSETHTCQPRSRPSSPTLPPPESEFKLASPEKLAGLASPSSGYSSQSETPTSSFPSAFFPGPLSPTSGKRKPKVPERKSSLCSLQQQQLSVRDPGISCRRETDFYAIPPSHLDLSALHSKHFPHRNQMHILHQNKQKAAIAAAAAAEARSAAAAAAEARSAAAAAAEARSAATAAAAAEACTAATASTKESATNTAPISAHLAITPTVLRSVQLRSICRPSDGNQGLDQDSSNLITRPKCPTIITDAPSSSNRHNRKPPAYKPPAAQVCDSQIPLVENIVFPQEEVRVRQERFGPGTYWAMTAFRTPVDPNCEKEVSLKRSSQCFQQEQDSRRCLDVQKTLSPERLKQDLNQLSRPDPSTQSMCLASTMPPPAYSEIQRSNFVLCNSPDKVPVSTQEASHSYTISDVQGREEDYETSGVATRSASQDIREEESTPDTEDYFSKDSTPSDNSSSPLTDDSRLDDDVFPSPNKLRTTEDLFAMIHRSKRKVLGRKDSGEVSGKGRPVVPPVTTPVSNPTVCPVIPAPPVPSNNSQRASGPIYRSAKKSSTSSEEFKLLLLKKGSRTDSSYRMSATEILKSPITPKTQAELLLEAMRQPEEIPLPQLDSTSSGDPLPSPFPKANSEGFSPKTLTMSAASRQGRSRIPPAANSSRYSTRSRLYTAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_009306119.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306119.2

ORF Insert Sequence:

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC CGGTCTCGAA TCTGGACATT 
GAGAGTAAAC TGACGTCGCA CTATCAGGCT CCATGGCATC AGCAACACAA TCTGTTCCAC 
ACTTGCACAC GGCCAGCGTG TCTGGAGGAG CTGCACCGAC ATGCCAAGCT CAACCTGCGG 
GCGCTGCACA GAGACCAGCA GCAGCGTCAG CGTTCAACCA GCAGAGAGCG CAGTCGGGTC 
ACTATATCAA TATCTGTGGC ACCGCCAATG CCAACCTTCC CTTCAACTCA CAAACTCAGA 
CGGCGAGAGC AGAGAGGTCG ACATTCACGC ATGGAGAGGA GCGTTAGAGA GAGTGATGTG 
CAAACTATCC AGAGGAAGGA GAGACCAGCC AAGGATTCTG AATTCAAACC TGTCCTCAGA 
AAGTATCAGC GCTCCCGCTC CCCCTCCCCT GTTCAGTGTT GTTACTTCAT TCCCTGGATT 
AGAAAGGCTG GAACCAACAC AGAAACGGAT GGAGAACTAC AAGTGATGAG CCATAGGCCT 
AAATGTCCTG TTCCCAATGC ACCTACCACC CTGGACAAAC AGACTAACTG GTCTAAAGCC 
CTGCCTCTTC CCACTCCAGA AGAAAGAATA AAGAATGACT CTCAAGTGAT TTCATCCTGT 
ATCATTCCAA TAAATGTTTC GGGTGTTGGA TTTGACAGAG AAGCCAGTGC TCGCTGCTCT 
CTTGTTCACT CGCAATCGGT TCTACAGAGA CGGCGGAAAC TCAGGAGGCG AAAAACGATC 
ACTGGGATCC CCAAACGAGT TCAACAAGAC ATGGATTCTG ACGAATCCCC TGTTGCTAGA 
GAAAGAACAG TGATAATCCA CGCCAATCCA CACAAGTCCC ACGAGTGGCA TGAGGAGCTC 
TCCTTGAGTG GCAGAGTATT GCATACTAAG GATTCGGGCT GTCAGACAGA TGACTTCTTG 
ATTACTGCTC CATCCCGAAG ACGCATCCGT GCCCAAAGAG GTCAGGGAAT TCCAGCGTCT 
CTCTCTCACT CCACTGGAAA CATTACCTCC CTCCCGGATC GTTCTGATAC GGTTTATGCT 
GCAGCATCAG CCACTCGTGT CCGTTCTAGG AGCCTTCCAC GTGAGGGTGG TCGGCTTCTG 
GATGAGGACC AAGATGACAG TGATGATGAT GATGATGAAG ATGATGATGA AGATGATGAA 
GAGGAAGATG AGGAGCTCTC TCCATATGAA GCCGAAGACT TCCTGCCAGG TCCCGGACAG 
AGAATTCCAA AGGATGAGGA AGAGAGTACT GACGACCAAG CCATGCCAGA GCTACAGTTT 
GGAAGTCTTA AGCGTATGCA GCATTCAGAG AGCCCTGACC ACACATGGAT TGAGAGAGGC 
AGATCCAGAC TCCCACGGAA AGTAGACATG GGGAGCTGTG AGATTTCCTC TAGTTCAGAT 
ACTTTCAGCA GCCCCATACA CTCTGCATCC ACTACAGGAG TGCTTGGCAG CCAGATTGAC 
CATAAAGAGG ATCACCAGTC CTCAAGTGGC AACTGGAGTG GAAGCAGCTC CACTTGCCCA 
TCGCAGACAT CCGAGACTCT TCCACCTGCT GCCTCCCCTC CCTTGACGGG ATCCTCACAC 
TGTGATTCAG AACTTTCCCT GAATACTGGG TCCCATGTCA TAGATGACAA CACTGGTTTC 
ATAATTGATC CCTATCACAT AGACAAGCCA CAGGGACAGG GGCAACGATC CAATTCATTT 
ACCTCATCAG CTACTGATCA GATGGATGAT GCAGGGGTCA GCACTGCTAG TGATGGGGAG 
TGGAACTGTG CCCAGGACCA GCAGGATCAG TCCTGCACCC AAGACTTCAG CCCAAAGCGT 
TCCAGAGAGG ATGAGAGTGG TGTCAGCTGC CCTAGTTATT CTAGTGGGGA AACTTACGAG 
AGCTTCAGTG ACCAAGCATC CGCTAGAAAA TCTGAGATGC ACTACATTGT CGACACTGAA 
GGATTCTATT CCTCCATGCA CTTTGACTCT GGTCTTAAGG GTAGCAGAAG CTACTACAAC 
TATGCAGCCA TTGACCCCGA CTGTGGCCCA AGTGGTAATA TAGCACCTGG CATGACTGAA 
TACCCTCAGC CAGAGTACAG AGCCACCAGG ACACTGGGCA GACCCTGTCT CTCCTTAAGA 
AAACCAAAGG CCAAGCCACC CCCACCAAAA CGTAGCTCTT CATTAAAGGA AACAAGCAGT 
GATGTGAACG TTCCAGAGCA GAACGAACCA AAGATTACTT GTGAGTTGCC ACTGCCCTTG 
TCTTCCAGGG AGATGAAACT GCAGCTGGAC TTGGCTGATT CTGCAGGGCA CCTTGAGACT 
GCAGGTCTTG AATCACTTGG TGCATGGGGA GTGGAAAATG TTAGGGACAT GCTTGACTGC 
TCCTCCCCAT TCAGTTCCTC AGACACACAT TCCTTCAAAG ATGAAGGTGC TGTGCAAGCA 
GACTATGCAG ACCTCTGGCT TCACAATGAC TTGAAATCAA ATGATCCTTA TCGGTCCCTC 
TCTAACTCTA GCTCTGCTAC AGGTACAACT GTTATTGAAT GCATCAAGTC ACCAGAAAGA 
TCAGAAACGC ACACCTGTCA ACCCAGGTCC AGACCTTCTT CCCCAACTCT TCCTCCACCT 
GAAAGTGAAT TTAAGCTTGC TTCTCCCGAA AAGCTGGCAG GCTTGGCGTC CCCTTCCAGT 
GGATATTCCA GTCAGTCTGA AACACCTACA TCTTCCTTCC CCTCTGCTTT CTTCCCAGGG 
CCCCTGTCTC CAACCAGTGG GAAGAGGAAG CCCAAAGTCC CTGAAAGGAA GTCCTCACTT 
TGTTCCTTAC AGCAGCAACA GCTTTCAGTC AGAGACCCAG GCATTTCCTG TAGGAGAGAA 
ACTGACTTCT ATGCCATACC CCCAAGTCAC CTTGACCTAA GTGCTCTTCA CAGTAAGCAC 
TTTCCTCACA GAAATCAAAT GCACATCCTC CACCAGAATA AGCAGAAAGC TGCCATAGCA 
GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA 
GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAACTGCAG CCGCTGCTGC CGAGGCCTGC 
ACTGCAGCTA CAGCTTCTAC TAAAGAGAGT GCAACAAATA CAGCACCCAT CTCAGCCCAT 
TTGGCTATTA CTCCAACGGT TCTTAGATCA GTGCAACTGC GATCTATATG TAGACCATCT 
GATGGCAACC AAGGGCTTGA TCAAGACAGT TCAAATCTCA TAACTCGTCC TAAGTGTCCC 
ACAATAATAA CTGATGCCCC ATCATCTAGC AACAGGCACA ACAGGAAGCC ACCAGCCTAC 
AAACCCCCTG CTGCACAGGT TTGTGATTCA CAGATTCCAC TGGTGGAAAA CATTGTTTTT 
CCACAAGAAG AAGTGAGAGT GAGACAGGAG AGGTTTGGTC CTGGTACTTA CTGGGCAATG 
ACTGCTTTCA GAACTCCTGT GGACCCTAAT TGTGAAAAAG AAGTCTCTTT AAAAAGGTCA 
TCTCAGTGTT TTCAGCAAGA ACAAGACAGC AGAAGGTGCC TAGATGTGCA AAAGACATTG 
TCACCAGAGA GACTAAAACA AGATTTGAAT CAACTATCGC GGCCAGACCC TTCAACACAG 
TCCATGTGTT TGGCCAGCAC AATGCCACCC CCTGCTTATA GTGAGATACA GAGGAGCAAT 
TTTGTACTGT GCAACAGTCC TGACAAAGTG CCTGTTTCAA CTCAAGAGGC ATCGCATTCT 
TACACAATCA GCGATGTACA AGGCAGAGAG GAGGATTATG AGACATCAGG TGTCGCAACC 
AGAAGTGCCT CACAGGACAT AAGGGAAGAA GAGTCAACCC CAGATACAGA GGACTATTTC 
AGTAAAGACT CTACTCCCAG TGATAATTCA TCCTCTCCTT TGACCGATGA CTCCAGACTT 
GATGATGATG TTTTCCCATC TCCCAATAAA CTCCGCACAA CTGAAGATCT TTTTGCTATG 
ATACACAGAT CTAAAAGGAA AGTGCTGGGA CGCAAAGATT CAGGGGAAGT CAGTGGAAAA 
GGTCGGCCTG TTGTACCACC TGTGACCACC CCTGTAAGCA ATCCTACTGT ATGCCCGGTC 
ATCCCTGCTC CCCCAGTTCC TTCAAACAAC TCCCAGCGAG CCTCAGGACC CATCTACAGG 
AGTGCCAAAA AGTCTAGTAC ATCCAGTGAG GAGTTCAAAC TCCTTCTGCT TAAGAAGGGT 
AGTCGCACAG ACTCAAGTTA TCGCATGTCT GCTACAGAGA TCCTTAAGAG CCCCATCACA 
CCCAAGACTC AAGCAGAGCT GCTGTTAGAG GCCATGAGGC AACCAGAGGA GATCCCCTTA 
CCCCAACTGG ATTCCACCAG CAGTGGAGAT CCACTTCCAA GTCCATTCCC TAAGGCCAAC 
AGTGAGGGAT TCTCCCCAAA AACACTCACC ATGTCAGCTG CCTCCAGACA AGGACGTTCT 
AGAATTCCAC CTGCAGCGAA CAGCAGTCGC TATAGCACGC GGAGTCGGCT GTACACTGCC 
CCAATGCAGG CCATATCAGA AGGAGAGACT GAGAACTCGG ATGGAAGTCC ACACGATGAC 
CGCTCCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsb ( XM_009306119.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304394.1 Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa43149
Clone ID Related Accession (Same CDS sequence)	XM_009306119.2 , XM_009306119.3
Accession Version	XM_009306119.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Nance-Horan syndrome protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306119.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC CGGTCTCGAA TCTGGACATT 
GAGAGTAAAC TGACGTCGCA CTATCAGGCT CCATGGCATC AGCAACACAA TCTGTTCCAC 
ACTTGCACAC GGCCAGCGTG TCTGGAGGAG CTGCACCGAC ATGCCAAGCT CAACCTGCGG 
GCGCTGCACA GAGACCAGCA GCAGCGTCAG CGTTCAACCA GCAGAGAGCG CAGTCGGGTC 
ACTATATCAA TATCTGTGGC ACCGCCAATG CCAACCTTCC CTTCAACTCA CAAACTCAGA 
CGGCGAGAGC AGAGAGGTCG ACATTCACGC ATGGAGAGGA GCGTTAGAGA GAGTGATGTG 
CAAACTATCC AGAGGAAGGA GAGACCAGCC AAGGATTCTG AATTCAAACC TGTCCTCAGA 
AAGTATCAGC GCTCCCGCTC CCCCTCCCCT GTTCAGTGTT GTTACTTCAT TCCCTGGATT 
AGAAAGGCTG GAACCAACAC AGAAACGGAT GGAGAACTAC AAGTGATGAG CCATAGGCCT 
AAATGTCCTG TTCCCAATGC ACCTACCACC CTGGACAAAC AGACTAACTG GTCTAAAGCC 
CTGCCTCTTC CCACTCCAGA AGAAAGAATA AAGAATGACT CTCAAGTGAT TTCATCCTGT 
ATCATTCCAA TAAATGTTTC GGGTGTTGGA TTTGACAGAG AAGCCAGTGC TCGCTGCTCT 
CTTGTTCACT CGCAATCGGT TCTACAGAGA CGGCGGAAAC TCAGGAGGCG AAAAACGATC 
ACTGGGATCC CCAAACGAGT TCAACAAGAC ATGGATTCTG ACGAATCCCC TGTTGCTAGA 
GAAAGAACAG TGATAATCCA CGCCAATCCA CACAAGTCCC ACGAGTGGCA TGAGGAGCTC 
TCCTTGAGTG GCAGAGTATT GCATACTAAG GATTCGGGCT GTCAGACAGA TGACTTCTTG 
ATTACTGCTC CATCCCGAAG ACGCATCCGT GCCCAAAGAG GTCAGGGAAT TCCAGCGTCT 
CTCTCTCACT CCACTGGAAA CATTACCTCC CTCCCGGATC GTTCTGATAC GGTTTATGCT 
GCAGCATCAG CCACTCGTGT CCGTTCTAGG AGCCTTCCAC GTGAGGGTGG TCGGCTTCTG 
GATGAGGACC AAGATGACAG TGATGATGAT GATGATGAAG ATGATGATGA AGATGATGAA 
GAGGAAGATG AGGAGCTCTC TCCATATGAA GCCGAAGACT TCCTGCCAGG TCCCGGACAG 
AGAATTCCAA AGGATGAGGA AGAGAGTACT GACGACCAAG CCATGCCAGA GCTACAGTTT 
GGAAGTCTTA AGCGTATGCA GCATTCAGAG AGCCCTGACC ACACATGGAT TGAGAGAGGC 
AGATCCAGAC TCCCACGGAA AGTAGACATG GGGAGCTGTG AGATTTCCTC TAGTTCAGAT 
ACTTTCAGCA GCCCCATACA CTCTGCATCC ACTACAGGAG TGCTTGGCAG CCAGATTGAC 
CATAAAGAGG ATCACCAGTC CTCAAGTGGC AACTGGAGTG GAAGCAGCTC CACTTGCCCA 
TCGCAGACAT CCGAGACTCT TCCACCTGCT GCCTCCCCTC CCTTGACGGG ATCCTCACAC 
TGTGATTCAG AACTTTCCCT GAATACTGGG TCCCATGTCA TAGATGACAA CACTGGTTTC 
ATAATTGATC CCTATCACAT AGACAAGCCA CAGGGACAGG GGCAACGATC CAATTCATTT 
ACCTCATCAG CTACTGATCA GATGGATGAT GCAGGGGTCA GCACTGCTAG TGATGGGGAG 
TGGAACTGTG CCCAGGACCA GCAGGATCAG TCCTGCACCC AAGACTTCAG CCCAAAGCGT 
TCCAGAGAGG ATGAGAGTGG TGTCAGCTGC CCTAGTTATT CTAGTGGGGA AACTTACGAG 
AGCTTCAGTG ACCAAGCATC CGCTAGAAAA TCTGAGATGC ACTACATTGT CGACACTGAA 
GGATTCTATT CCTCCATGCA CTTTGACTCT GGTCTTAAGG GTAGCAGAAG CTACTACAAC 
TATGCAGCCA TTGACCCCGA CTGTGGCCCA AGTGGTAATA TAGCACCTGG CATGACTGAA 
TACCCTCAGC CAGAGTACAG AGCCACCAGG ACACTGGGCA GACCCTGTCT CTCCTTAAGA 
AAACCAAAGG CCAAGCCACC CCCACCAAAA CGTAGCTCTT CATTAAAGGA AACAAGCAGT 
GATGTGAACG TTCCAGAGCA GAACGAACCA AAGATTACTT GTGAGTTGCC ACTGCCCTTG 
TCTTCCAGGG AGATGAAACT GCAGCTGGAC TTGGCTGATT CTGCAGGGCA CCTTGAGACT 
GCAGGTCTTG AATCACTTGG TGCATGGGGA GTGGAAAATG TTAGGGACAT GCTTGACTGC 
TCCTCCCCAT TCAGTTCCTC AGACACACAT TCCTTCAAAG ATGAAGGTGC TGTGCAAGCA 
GACTATGCAG ACCTCTGGCT TCACAATGAC TTGAAATCAA ATGATCCTTA TCGGTCCCTC 
TCTAACTCTA GCTCTGCTAC AGGTACAACT GTTATTGAAT GCATCAAGTC ACCAGAAAGA 
TCAGAAACGC ACACCTGTCA ACCCAGGTCC AGACCTTCTT CCCCAACTCT TCCTCCACCT 
GAAAGTGAAT TTAAGCTTGC TTCTCCCGAA AAGCTGGCAG GCTTGGCGTC CCCTTCCAGT 
GGATATTCCA GTCAGTCTGA AACACCTACA TCTTCCTTCC CCTCTGCTTT CTTCCCAGGG 
CCCCTGTCTC CAACCAGTGG GAAGAGGAAG CCCAAAGTCC CTGAAAGGAA GTCCTCACTT 
TGTTCCTTAC AGCAGCAACA GCTTTCAGTC AGAGACCCAG GCATTTCCTG TAGGAGAGAA 
ACTGACTTCT ATGCCATACC CCCAAGTCAC CTTGACCTAA GTGCTCTTCA CAGTAAGCAC 
TTTCCTCACA GAAATCAAAT GCACATCCTC CACCAGAATA AGCAGAAAGC TGCCATAGCA 
GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA 
GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAACTGCAG CCGCTGCTGC CGAGGCCTGC 
ACTGCAGCTA CAGCTTCTAC TAAAGAGAGT GCAACAAATA CAGCACCCAT CTCAGCCCAT 
TTGGCTATTA CTCCAACGGT TCTTAGATCA GTGCAACTGC GATCTATATG TAGACCATCT 
GATGGCAACC AAGGGCTTGA TCAAGACAGT TCAAATCTCA TAACTCGTCC TAAGTGTCCC 
ACAATAATAA CTGATGCCCC ATCATCTAGC AACAGGCACA ACAGGAAGCC ACCAGCCTAC 
AAACCCCCTG CTGCACAGGT TTGTGATTCA CAGATTCCAC TGGTGGAAAA CATTGTTTTT 
CCACAAGAAG AAGTGAGAGT GAGACAGGAG AGGTTTGGTC CTGGTACTTA CTGGGCAATG 
ACTGCTTTCA GAACTCCTGT GGACCCTAAT TGTGAAAAAG AAGTCTCTTT AAAAAGGTCA 
TCTCAGTGTT TTCAGCAAGA ACAAGACAGC AGAAGGTGCC TAGATGTGCA AAAGACATTG 
TCACCAGAGA GACTAAAACA AGATTTGAAT CAACTATCGC GGCCAGACCC TTCAACACAG 
TCCATGTGTT TGGCCAGCAC AATGCCACCC CCTGCTTATA GTGAGATACA GAGGAGCAAT 
TTTGTACTGT GCAACAGTCC TGACAAAGTG CCTGTTTCAA CTCAAGAGGC ATCGCATTCT 
TACACAATCA GCGATGTACA AGGCAGAGAG GAGGATTATG AGACATCAGG TGTCGCAACC 
AGAAGTGCCT CACAGGACAT AAGGGAAGAA GAGTCAACCC CAGATACAGA GGACTATTTC 
AGTAAAGACT CTACTCCCAG TGATAATTCA TCCTCTCCTT TGACCGATGA CTCCAGACTT 
GATGATGATG TTTTCCCATC TCCCAATAAA CTCCGCACAA CTGAAGATCT TTTTGCTATG 
ATACACAGAT CTAAAAGGAA AGTGCTGGGA CGCAAAGATT CAGGGGAAGT CAGTGGAAAA 
GGTCGGCCTG TTGTACCACC TGTGACCACC CCTGTAAGCA ATCCTACTGT ATGCCCGGTC 
ATCCCTGCTC CCCCAGTTCC TTCAAACAAC TCCCAGCGAG CCTCAGGACC CATCTACAGG 
AGTGCCAAAA AGTCTAGTAC ATCCAGTGAG GAGTTCAAAC TCCTTCTGCT TAAGAAGGGT 
AGTCGCACAG ACTCAAGTTA TCGCATGTCT GCTACAGAGA TCCTTAAGAG CCCCATCACA 
CCCAAGACTC AAGCAGAGCT GCTGTTAGAG GCCATGAGGC AACCAGAGGA GATCCCCTTA 
CCCCAACTGG ATTCCACCAG CAGTGGAGAT CCACTTCCAA GTCCATTCCC TAAGGCCAAC 
AGTGAGGGAT TCTCCCCAAA AACACTCACC ATGTCAGCTG CCTCCAGACA AGGACGTTCT 
AGAATTCCAC CTGCAGCGAA CAGCAGTCGC TATAGCACGC GGAGTCGGCT GTACACTGCC 
CCAATGCAGG CCATATCAGA AGGAGAGACT GAGAACTCGG ATGGAAGTCC ACACGATGAC 
CGCTCCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304394.1
CDS	321..5192
Translation	MPFAKRIVEPQLLVRHPIPNDEGLLFEDLCSITNVALSRTLRQLSDLSKHACSLFQELENEIVSTNQRVWALQNKIGKIQQTASALDPKMEAVPVSNLDIESKLTSHYQAPWHQQHNLFHTCTRPACLEELHRHAKLNLRALHRDQQQRQRSTSRERSRVTISISVAPPMPTFPSTHKLRRREQRGRHSRMERSVRESDVQTIQRKERPAKDSEFKPVLRKYQRSRSPSPVQCCYFIPWIRKAGTNTETDGELQVMSHRPKCPVPNAPTTLDKQTNWSKALPLPTPEERIKNDSQVISSCIIPINVSGVGFDREASARCSLVHSQSVLQRRRKLRRRKTITGIPKRVQQDMDSDESPVARERTVIIHANPHKSHEWHEELSLSGRVLHTKDSGCQTDDFLITAPSRRRIRAQRGQGIPASLSHSTGNITSLPDRSDTVYAAASATRVRSRSLPREGGRLLDEDQDDSDDDDDEDDDEDDEEEDEELSPYEAEDFLPGPGQRIPKDEEESTDDQAMPELQFGSLKRMQHSESPDHTWIERGRSRLPRKVDMGSCEISSSSDTFSSPIHSASTTGVLGSQIDHKEDHQSSSGNWSGSSSTCPSQTSETLPPAASPPLTGSSHCDSELSLNTGSHVIDDNTGFIIDPYHIDKPQGQGQRSNSFTSSATDQMDDAGVSTASDGEWNCAQDQQDQSCTQDFSPKRSREDESGVSCPSYSSGETYESFSDQASARKSEMHYIVDTEGFYSSMHFDSGLKGSRSYYNYAAIDPDCGPSGNIAPGMTEYPQPEYRATRTLGRPCLSLRKPKAKPPPPKRSSSLKETSSDVNVPEQNEPKITCELPLPLSSREMKLQLDLADSAGHLETAGLESLGAWGVENVRDMLDCSSPFSSSDTHSFKDEGAVQADYADLWLHNDLKSNDPYRSLSNSSSATGTTVIECIKSPERSETHTCQPRSRPSSPTLPPPESEFKLASPEKLAGLASPSSGYSSQSETPTSSFPSAFFPGPLSPTSGKRKPKVPERKSSLCSLQQQQLSVRDPGISCRRETDFYAIPPSHLDLSALHSKHFPHRNQMHILHQNKQKAAIAAAAAAEARSAAAAAAEARSAAAAAAEARSAATAAAAAEACTAATASTKESATNTAPISAHLAITPTVLRSVQLRSICRPSDGNQGLDQDSSNLITRPKCPTIITDAPSSSNRHNRKPPAYKPPAAQVCDSQIPLVENIVFPQEEVRVRQERFGPGTYWAMTAFRTPVDPNCEKEVSLKRSSQCFQQEQDSRRCLDVQKTLSPERLKQDLNQLSRPDPSTQSMCLASTMPPPAYSEIQRSNFVLCNSPDKVPVSTQEASHSYTISDVQGREEDYETSGVATRSASQDIREEESTPDTEDYFSKDSTPSDNSSSPLTDDSRLDDDVFPSPNKLRTTEDLFAMIHRSKRKVLGRKDSGEVSGKGRPVVPPVTTPVSNPTVCPVIPAPPVPSNNSQRASGPIYRSAKKSSTSSEEFKLLLLKKGSRTDSSYRMSATEILKSPITPKTQAELLLEAMRQPEEIPLPQLDSTSSGDPLPSPFPKANSEGFSPKTLTMSAASRQGRSRIPPAANSSRYSTRSRLYTAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_009306119.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306119.3

ORF Insert Sequence:

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC CGGTCTCGAA TCTGGACATT 
GAGAGTAAAC TGACGTCGCA CTATCAGGCT CCATGGCATC AGCAACACAA TCTGTTCCAC 
ACTTGCACAC GGCCAGCGTG TCTGGAGGAG CTGCACCGAC ATGCCAAGCT CAACCTGCGG 
GCGCTGCACA GAGACCAGCA GCAGCGTCAG CGTTCAACCA GCAGAGAGCG CAGTCGGGTC 
ACTATATCAA TATCTGTGGC ACCGCCAATG CCAACCTTCC CTTCAACTCA CAAACTCAGA 
CGGCGAGAGC AGAGAGGTCG ACATTCACGC ATGGAGAGGA GCGTTAGAGA GAGTGATGTG 
CAAACTATCC AGAGGAAGGA GAGACCAGCC AAGGATTCTG AATTCAAACC TGTCCTCAGA 
AAGTATCAGC GCTCCCGCTC CCCCTCCCCT GTTCAGTGTT GTTACTTCAT TCCCTGGATT 
AGAAAGGCTG GAACCAACAC AGAAACGGAT GGAGAACTAC AAGTGATGAG CCATAGGCCT 
AAATGTCCTG TTCCCAATGC ACCTACCACC CTGGACAAAC AGACTAACTG GTCTAAAGCC 
CTGCCTCTTC CCACTCCAGA AGAAAGAATA AAGAATGACT CTCAAGTGAT TTCATCCTGT 
ATCATTCCAA TAAATGTTTC GGGTGTTGGA TTTGACAGAG AAGCCAGTGC TCGCTGCTCT 
CTTGTTCACT CGCAATCGGT TCTACAGAGA CGGCGGAAAC TCAGGAGGCG AAAAACGATC 
ACTGGGATCC CCAAACGAGT TCAACAAGAC ATGGATTCTG ACGAATCCCC TGTTGCTAGA 
GAAAGAACAG TGATAATCCA CGCCAATCCA CACAAGTCCC ACGAGTGGCA TGAGGAGCTC 
TCCTTGAGTG GCAGAGTATT GCATACTAAG GATTCGGGCT GTCAGACAGA TGACTTCTTG 
ATTACTGCTC CATCCCGAAG ACGCATCCGT GCCCAAAGAG GTCAGGGAAT TCCAGCGTCT 
CTCTCTCACT CCACTGGAAA CATTACCTCC CTCCCGGATC GTTCTGATAC GGTTTATGCT 
GCAGCATCAG CCACTCGTGT CCGTTCTAGG AGCCTTCCAC GTGAGGGTGG TCGGCTTCTG 
GATGAGGACC AAGATGACAG TGATGATGAT GATGATGAAG ATGATGATGA AGATGATGAA 
GAGGAAGATG AGGAGCTCTC TCCATATGAA GCCGAAGACT TCCTGCCAGG TCCCGGACAG 
AGAATTCCAA AGGATGAGGA AGAGAGTACT GACGACCAAG CCATGCCAGA GCTACAGTTT 
GGAAGTCTTA AGCGTATGCA GCATTCAGAG AGCCCTGACC ACACATGGAT TGAGAGAGGC 
AGATCCAGAC TCCCACGGAA AGTAGACATG GGGAGCTGTG AGATTTCCTC TAGTTCAGAT 
ACTTTCAGCA GCCCCATACA CTCTGCATCC ACTACAGGAG TGCTTGGCAG CCAGATTGAC 
CATAAAGAGG ATCACCAGTC CTCAAGTGGC AACTGGAGTG GAAGCAGCTC CACTTGCCCA 
TCGCAGACAT CCGAGACTCT TCCACCTGCT GCCTCCCCTC CCTTGACGGG ATCCTCACAC 
TGTGATTCAG AACTTTCCCT GAATACTGGG TCCCATGTCA TAGATGACAA CACTGGTTTC 
ATAATTGATC CCTATCACAT AGACAAGCCA CAGGGACAGG GGCAACGATC CAATTCATTT 
ACCTCATCAG CTACTGATCA GATGGATGAT GCAGGGGTCA GCACTGCTAG TGATGGGGAG 
TGGAACTGTG CCCAGGACCA GCAGGATCAG TCCTGCACCC AAGACTTCAG CCCAAAGCGT 
TCCAGAGAGG ATGAGAGTGG TGTCAGCTGC CCTAGTTATT CTAGTGGGGA AACTTACGAG 
AGCTTCAGTG ACCAAGCATC CGCTAGAAAA TCTGAGATGC ACTACATTGT CGACACTGAA 
GGATTCTATT CCTCCATGCA CTTTGACTCT GGTCTTAAGG GTAGCAGAAG CTACTACAAC 
TATGCAGCCA TTGACCCCGA CTGTGGCCCA AGTGGTAATA TAGCACCTGG CATGACTGAA 
TACCCTCAGC CAGAGTACAG AGCCACCAGG ACACTGGGCA GACCCTGTCT CTCCTTAAGA 
AAACCAAAGG CCAAGCCACC CCCACCAAAA CGTAGCTCTT CATTAAAGGA AACAAGCAGT 
GATGTGAACG TTCCAGAGCA GAACGAACCA AAGATTACTT GTGAGTTGCC ACTGCCCTTG 
TCTTCCAGGG AGATGAAACT GCAGCTGGAC TTGGCTGATT CTGCAGGGCA CCTTGAGACT 
GCAGGTCTTG AATCACTTGG TGCATGGGGA GTGGAAAATG TTAGGGACAT GCTTGACTGC 
TCCTCCCCAT TCAGTTCCTC AGACACACAT TCCTTCAAAG ATGAAGGTGC TGTGCAAGCA 
GACTATGCAG ACCTCTGGCT TCACAATGAC TTGAAATCAA ATGATCCTTA TCGGTCCCTC 
TCTAACTCTA GCTCTGCTAC AGGTACAACT GTTATTGAAT GCATCAAGTC ACCAGAAAGA 
TCAGAAACGC ACACCTGTCA ACCCAGGTCC AGACCTTCTT CCCCAACTCT TCCTCCACCT 
GAAAGTGAAT TTAAGCTTGC TTCTCCCGAA AAGCTGGCAG GCTTGGCGTC CCCTTCCAGT 
GGATATTCCA GTCAGTCTGA AACACCTACA TCTTCCTTCC CCTCTGCTTT CTTCCCAGGG 
CCCCTGTCTC CAACCAGTGG GAAGAGGAAG CCCAAAGTCC CTGAAAGGAA GTCCTCACTT 
TGTTCCTTAC AGCAGCAACA GCTTTCAGTC AGAGACCCAG GCATTTCCTG TAGGAGAGAA 
ACTGACTTCT ATGCCATACC CCCAAGTCAC CTTGACCTAA GTGCTCTTCA CAGTAAGCAC 
TTTCCTCACA GAAATCAAAT GCACATCCTC CACCAGAATA AGCAGAAAGC TGCCATAGCA 
GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA 
GCAGCTGCTG CAGCAGAAGC TCGCAGTGCA GCAACTGCAG CCGCTGCTGC CGAGGCCTGC 
ACTGCAGCTA CAGCTTCTAC TAAAGAGAGT GCAACAAATA CAGCACCCAT CTCAGCCCAT 
TTGGCTATTA CTCCAACGGT TCTTAGATCA GTGCAACTGC GATCTATATG TAGACCATCT 
GATGGCAACC AAGGGCTTGA TCAAGACAGT TCAAATCTCA TAACTCGTCC TAAGTGTCCC 
ACAATAATAA CTGATGCCCC ATCATCTAGC AACAGGCACA ACAGGAAGCC ACCAGCCTAC 
AAACCCCCTG CTGCACAGGT TTGTGATTCA CAGATTCCAC TGGTGGAAAA CATTGTTTTT 
CCACAAGAAG AAGTGAGAGT GAGACAGGAG AGGTTTGGTC CTGGTACTTA CTGGGCAATG 
ACTGCTTTCA GAACTCCTGT GGACCCTAAT TGTGAAAAAG AAGTCTCTTT AAAAAGGTCA 
TCTCAGTGTT TTCAGCAAGA ACAAGACAGC AGAAGGTGCC TAGATGTGCA AAAGACATTG 
TCACCAGAGA GACTAAAACA AGATTTGAAT CAACTATCGC GGCCAGACCC TTCAACACAG 
TCCATGTGTT TGGCCAGCAC AATGCCACCC CCTGCTTATA GTGAGATACA GAGGAGCAAT 
TTTGTACTGT GCAACAGTCC TGACAAAGTG CCTGTTTCAA CTCAAGAGGC ATCGCATTCT 
TACACAATCA GCGATGTACA AGGCAGAGAG GAGGATTATG AGACATCAGG TGTCGCAACC 
AGAAGTGCCT CACAGGACAT AAGGGAAGAA GAGTCAACCC CAGATACAGA GGACTATTTC 
AGTAAAGACT CTACTCCCAG TGATAATTCA TCCTCTCCTT TGACCGATGA CTCCAGACTT 
GATGATGATG TTTTCCCATC TCCCAATAAA CTCCGCACAA CTGAAGATCT TTTTGCTATG 
ATACACAGAT CTAAAAGGAA AGTGCTGGGA CGCAAAGATT CAGGGGAAGT CAGTGGAAAA 
GGTCGGCCTG TTGTACCACC TGTGACCACC CCTGTAAGCA ATCCTACTGT ATGCCCGGTC 
ATCCCTGCTC CCCCAGTTCC TTCAAACAAC TCCCAGCGAG CCTCAGGACC CATCTACAGG 
AGTGCCAAAA AGTCTAGTAC ATCCAGTGAG GAGTTCAAAC TCCTTCTGCT TAAGAAGGGT 
AGTCGCACAG ACTCAAGTTA TCGCATGTCT GCTACAGAGA TCCTTAAGAG CCCCATCACA 
CCCAAGACTC AAGCAGAGCT GCTGTTAGAG GCCATGAGGC AACCAGAGGA GATCCCCTTA 
CCCCAACTGG ATTCCACCAG CAGTGGAGAT CCACTTCCAA GTCCATTCCC TAAGGCCAAC 
AGTGAGGGAT TCTCCCCAAA AACACTCACC ATGTCAGCTG CCTCCAGACA AGGACGTTCT 
AGAATTCCAC CTGCAGCGAA CAGCAGTCGC TATAGCACGC GGAGTCGGCT GTACACTGCC 
CCAATGCAGG CCATATCAGA AGGAGAGACT GAGAACTCGG ATGGAAGTCC ACACGATGAC 
CGCTCCTCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsb ( XM_009306120.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304395.1 Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X3, mRNA.

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CloneID	ODa43150
Clone ID Related Accession (Same CDS sequence)	XM_009306120.2 , XM_009306120.3
Accession Version	XM_009306120.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	Nance-Horan syndrome protein isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306120.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA 
GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT 
CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA 
CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC 
AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC 
CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG 
AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT 
GAATTCAAAC CTGTCCTCAG AAAGGCTGGA ACCAACACAG AAACGGATGG AGAACTACAA 
GTGATGAGCC ATAGGCCTAA ATGTCCTGTT CCCAATGCAC CTACCACCCT GGACAAACAG 
ACTAACTGGT CTAAAGCCCT GCCTCTTCCC ACTCCAGAAG AAAGAATAAA GAATGACTCT 
CAAGTGATTT CATCCTGTAT CATTCCAATA AATGTTTCGG GTGTTGGATT TGACAGAGAA 
GCCAGTGCTC GCTGCTCTCT TGTTCACTCG CAATCGGTTC TACAGAGACG GCGGAAACTC 
AGGAGGCGAA AAACGATCAC TGGGATCCCC AAACGAGTTC AACAAGACAT GGATTCTGAC 
GAATCCCCTG TTGCTAGAGA AAGAACAGTG ATAATCCACG CCAATCCACA CAAGTCCCAC 
GAGTGGCATG AGGAGCTCTC CTTGAGTGGC AGAGTATTGC ATACTAAGGA TTCGGGCTGT 
CAGACAGATG ACTTCTTGAT TACTGCTCCA TCCCGAAGAC GCATCCGTGC CCAAAGAGGT 
CAGGGAATTC CAGCGTCTCT CTCTCACTCC ACTGGAAACA TTACCTCCCT CCCGGATCGT 
TCTGATACGG TTTATGCTGC AGCATCAGCC ACTCGTGTCC GTTCTAGGAG CCTTCCACGT 
GAGGGTGGTC GGCTTCTGGA TGAGGACCAA GATGACAGTG ATGATGATGA TGATGAAGAT 
GATGATGAAG ATGATGAAGA GGAAGATGAG GAGCTCTCTC CATATGAAGC CGAAGACTTC 
CTGCCAGGTC CCGGACAGAG AATTCCAAAG GATGAGGAAG AGAGTACTGA CGACCAAGCC 
ATGCCAGAGC TACAGTTTGG AAGTCTTAAG CGTATGCAGC ATTCAGAGAG CCCTGACCAC 
ACATGGATTG AGAGAGGCAG ATCCAGACTC CCACGGAAAG TAGACATGGG GAGCTGTGAG 
ATTTCCTCTA GTTCAGATAC TTTCAGCAGC CCCATACACT CTGCATCCAC TACAGGAGTG 
CTTGGCAGCC AGATTGACCA TAAAGAGGAT CACCAGTCCT CAAGTGGCAA CTGGAGTGGA 
AGCAGCTCCA CTTGCCCATC GCAGACATCC GAGACTCTTC CACCTGCTGC CTCCCCTCCC 
TTGACGGGAT CCTCACACTG TGATTCAGAA CTTTCCCTGA ATACTGGGTC CCATGTCATA 
GATGACAACA CTGGTTTCAT AATTGATCCC TATCACATAG ACAAGCCACA GGGACAGGGG 
CAACGATCCA ATTCATTTAC CTCATCAGCT ACTGATCAGA TGGATGATGC AGGGGTCAGC 
ACTGCTAGTG ATGGGGAGTG GAACTGTGCC CAGGACCAGC AGGATCAGTC CTGCACCCAA 
GACTTCAGCC CAAAGCGTTC CAGAGAGGAT GAGAGTGGTG TCAGCTGCCC TAGTTATTCT 
AGTGGGGAAA CTTACGAGAG CTTCAGTGAC CAAGCATCCG CTAGAAAATC TGAGATGCAC 
TACATTGTCG ACACTGAAGG ATTCTATTCC TCCATGCACT TTGACTCTGG TCTTAAGGGT 
AGCAGAAGCT ACTACAACTA TGCAGCCATT GACCCCGACT GTGGCCCAAG TGGTAATATA 
GCACCTGGCA TGACTGAATA CCCTCAGCCA GAGTACAGAG CCACCAGGAC ACTGGGCAGA 
CCCTGTCTCT CCTTAAGAAA ACCAAAGGCC AAGCCACCCC CACCAAAACG TAGCTCTTCA 
TTAAAGGAAA CAAGCAGTGA TGTGAACGTT CCAGAGCAGA ACGAACCAAA GATTACTTGT 
GAGTTGCCAC TGCCCTTGTC TTCCAGGGAG ATGAAACTGC AGCTGGACTT GGCTGATTCT 
GCAGGGCACC TTGAGACTGC AGGTCTTGAA TCACTTGGTG CATGGGGAGT GGAAAATGTT 
AGGGACATGC TTGACTGCTC CTCCCCATTC AGTTCCTCAG ACACACATTC CTTCAAAGAT 
GAAGGTGCTG TGCAAGCAGA CTATGCAGAC CTCTGGCTTC ACAATGACTT GAAATCAAAT 
GATCCTTATC GGTCCCTCTC TAACTCTAGC TCTGCTACAG GTACAACTGT TATTGAATGC 
ATCAAGTCAC CAGAAAGATC AGAAACGCAC ACCTGTCAAC CCAGGTCCAG ACCTTCTTCC 
CCAACTCTTC CTCCACCTGA AAGTGAATTT AAGCTTGCTT CTCCCGAAAA GCTGGCAGGC 
TTGGCGTCCC CTTCCAGTGG ATATTCCAGT CAGTCTGAAA CACCTACATC TTCCTTCCCC 
TCTGCTTTCT TCCCAGGGCC CCTGTCTCCA ACCAGTGGGA AGAGGAAGCC CAAAGTCCCT 
GAAAGGAAGT CCTCACTTTG TTCCTTACAG CAGCAACAGC TTTCAGTCAG AGACCCAGGC 
ATTTCCTGTA GGAGAGAAAC TGACTTCTAT GCCATACCCC CAAGTCACCT TGACCTAAGT 
GCTCTTCACA GTAAGCACTT TCCTCACAGA AATCAAATGC ACATCCTCCA CCAGAATAAG 
CAGAAAGCTG CCATAGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA 
GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AACTGCAGCC 
GCTGCTGCCG AGGCCTGCAC TGCAGCTACA GCTTCTACTA AAGAGAGTGC AACAAATACA 
GCACCCATCT CAGCCCATTT GGCTATTACT CCAACGGTTC TTAGATCAGT GCAACTGCGA 
TCTATATGTA GACCATCTGA TGGCAACCAA GGGCTTGATC AAGACAGTTC AAATCTCATA 
ACTCGTCCTA AGTGTCCCAC AATAATAACT GATGCCCCAT CATCTAGCAA CAGGCACAAC 
AGGAAGCCAC CAGCCTACAA ACCCCCTGCT GCACAGGTTT GTGATTCACA GATTCCACTG 
GTGGAAAACA TTGTTTTTCC ACAAGAAGAA GTGAGAGTGA GACAGGAGAG GTTTGGTCCT 
GGTACTTACT GGGCAATGAC TGCTTTCAGA ACTCCTGTGG ACCCTAATTG TGAAAAAGAA 
GTCTCTTTAA AAAGGTCATC TCAGTGTTTT CAGCAAGAAC AAGACAGCAG AAGGTGCCTA 
GATGTGCAAA AGACATTGTC ACCAGAGAGA CTAAAACAAG ATTTGAATCA ACTATCGCGG 
CCAGACCCTT CAACACAGTC CATGTGTTTG GCCAGCACAA TGCCACCCCC TGCTTATAGT 
GAGATACAGA GGAGCAATTT TGTACTGTGC AACAGTCCTG ACAAAGTGCC TGTTTCAACT 
CAAGAGGCAT CGCATTCTTA CACAATCAGC GATGTACAAG GCAGAGAGGA GGATTATGAG 
ACATCAGGTG TCGCAACCAG AAGTGCCTCA CAGGACATAA GGGAAGAAGA GTCAACCCCA 
GATACAGAGG ACTATTTCAG TAAAGACTCT ACTCCCAGTG ATAATTCATC CTCTCCTTTG 
ACCGATGACT CCAGACTTGA TGATGATGTT TTCCCATCTC CCAATAAACT CCGCACAACT 
GAAGATCTTT TTGCTATGAT ACACAGATCT AAAAGGAAAG TGCTGGGACG CAAAGATTCA 
GGGGAAGTCA GTGGAAAAGG TCGGCCTGTT GTACCACCTG TGACCACCCC TGTAAGCAAT 
CCTACTGTAT GCCCGGTCAT CCCTGCTCCC CCAGTTCCTT CAAACAACTC CCAGCGAGCC 
TCAGGACCCA TCTACAGGAG TGCCAAAAAG TCTAGTACAT CCAGTGAGGA GTTCAAACTC 
CTTCTGCTTA AGAAGGGTAG TCGCACAGAC TCAAGTTATC GCATGTCTGC TACAGAGATC 
CTTAAGAGCC CCATCACACC CAAGACTCAA GCAGAGCTGC TGTTAGAGGC CATGAGGCAA 
CCAGAGGAGA TCCCCTTACC CCAACTGGAT TCCACCAGCA GTGGAGATCC ACTTCCAAGT 
CCATTCCCTA AGGCCAACAG TGAGGGATTC TCCCCAAAAA CACTCACCAT GTCAGCTGCC 
TCCAGACAAG GACGTTCTAG AATTCCACCT GCAGCGAACA GCAGTCGCTA TAGCACGCGG 
AGTCGGCTGT ACACTGCCCC AATGCAGGCC ATATCAGAAG GAGAGACTGA GAACTCGGAT 
GGAAGTCCAC ACGATGACCG CTCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304395.1
CDS	291..5120
Translation	MPFAKRIVEPQLLVRHPIPNDEGLLFEDLCSITNVALSRTLRQLSDLSKHACSLFQELENEIVSTNQRVWALQNKIGKIQQTASALDPKMEAVRFRSISIAVSNLDIESKLTSHYQAPWHQQHNLFHTCTRPACLEELHRHAKLNLRALHRDQQQRQRSTSRERSRVTISISVAPPMPTFPSTHKLRRREQRGRHSRMERSVRESDVQTIQRKERPAKDSEFKPVLRKAGTNTETDGELQVMSHRPKCPVPNAPTTLDKQTNWSKALPLPTPEERIKNDSQVISSCIIPINVSGVGFDREASARCSLVHSQSVLQRRRKLRRRKTITGIPKRVQQDMDSDESPVARERTVIIHANPHKSHEWHEELSLSGRVLHTKDSGCQTDDFLITAPSRRRIRAQRGQGIPASLSHSTGNITSLPDRSDTVYAAASATRVRSRSLPREGGRLLDEDQDDSDDDDDEDDDEDDEEEDEELSPYEAEDFLPGPGQRIPKDEEESTDDQAMPELQFGSLKRMQHSESPDHTWIERGRSRLPRKVDMGSCEISSSSDTFSSPIHSASTTGVLGSQIDHKEDHQSSSGNWSGSSSTCPSQTSETLPPAASPPLTGSSHCDSELSLNTGSHVIDDNTGFIIDPYHIDKPQGQGQRSNSFTSSATDQMDDAGVSTASDGEWNCAQDQQDQSCTQDFSPKRSREDESGVSCPSYSSGETYESFSDQASARKSEMHYIVDTEGFYSSMHFDSGLKGSRSYYNYAAIDPDCGPSGNIAPGMTEYPQPEYRATRTLGRPCLSLRKPKAKPPPPKRSSSLKETSSDVNVPEQNEPKITCELPLPLSSREMKLQLDLADSAGHLETAGLESLGAWGVENVRDMLDCSSPFSSSDTHSFKDEGAVQADYADLWLHNDLKSNDPYRSLSNSSSATGTTVIECIKSPERSETHTCQPRSRPSSPTLPPPESEFKLASPEKLAGLASPSSGYSSQSETPTSSFPSAFFPGPLSPTSGKRKPKVPERKSSLCSLQQQQLSVRDPGISCRRETDFYAIPPSHLDLSALHSKHFPHRNQMHILHQNKQKAAIAAAAAAEARSAAAAAAEARSAAAAAAEARSAATAAAAAEACTAATASTKESATNTAPISAHLAITPTVLRSVQLRSICRPSDGNQGLDQDSSNLITRPKCPTIITDAPSSSNRHNRKPPAYKPPAAQVCDSQIPLVENIVFPQEEVRVRQERFGPGTYWAMTAFRTPVDPNCEKEVSLKRSSQCFQQEQDSRRCLDVQKTLSPERLKQDLNQLSRPDPSTQSMCLASTMPPPAYSEIQRSNFVLCNSPDKVPVSTQEASHSYTISDVQGREEDYETSGVATRSASQDIREEESTPDTEDYFSKDSTPSDNSSSPLTDDSRLDDDVFPSPNKLRTTEDLFAMIHRSKRKVLGRKDSGEVSGKGRPVVPPVTTPVSNPTVCPVIPAPPVPSNNSQRASGPIYRSAKKSSTSSEEFKLLLLKKGSRTDSSYRMSATEILKSPITPKTQAELLLEAMRQPEEIPLPQLDSTSSGDPLPSPFPKANSEGFSPKTLTMSAASRQGRSRIPPAANSSRYSTRSRLYTAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_009306120.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306120.2

ORF Insert Sequence:

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA 
GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT 
CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA 
CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC 
AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC 
CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG 
AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT 
GAATTCAAAC CTGTCCTCAG AAAGGCTGGA ACCAACACAG AAACGGATGG AGAACTACAA 
GTGATGAGCC ATAGGCCTAA ATGTCCTGTT CCCAATGCAC CTACCACCCT GGACAAACAG 
ACTAACTGGT CTAAAGCCCT GCCTCTTCCC ACTCCAGAAG AAAGAATAAA GAATGACTCT 
CAAGTGATTT CATCCTGTAT CATTCCAATA AATGTTTCGG GTGTTGGATT TGACAGAGAA 
GCCAGTGCTC GCTGCTCTCT TGTTCACTCG CAATCGGTTC TACAGAGACG GCGGAAACTC 
AGGAGGCGAA AAACGATCAC TGGGATCCCC AAACGAGTTC AACAAGACAT GGATTCTGAC 
GAATCCCCTG TTGCTAGAGA AAGAACAGTG ATAATCCACG CCAATCCACA CAAGTCCCAC 
GAGTGGCATG AGGAGCTCTC CTTGAGTGGC AGAGTATTGC ATACTAAGGA TTCGGGCTGT 
CAGACAGATG ACTTCTTGAT TACTGCTCCA TCCCGAAGAC GCATCCGTGC CCAAAGAGGT 
CAGGGAATTC CAGCGTCTCT CTCTCACTCC ACTGGAAACA TTACCTCCCT CCCGGATCGT 
TCTGATACGG TTTATGCTGC AGCATCAGCC ACTCGTGTCC GTTCTAGGAG CCTTCCACGT 
GAGGGTGGTC GGCTTCTGGA TGAGGACCAA GATGACAGTG ATGATGATGA TGATGAAGAT 
GATGATGAAG ATGATGAAGA GGAAGATGAG GAGCTCTCTC CATATGAAGC CGAAGACTTC 
CTGCCAGGTC CCGGACAGAG AATTCCAAAG GATGAGGAAG AGAGTACTGA CGACCAAGCC 
ATGCCAGAGC TACAGTTTGG AAGTCTTAAG CGTATGCAGC ATTCAGAGAG CCCTGACCAC 
ACATGGATTG AGAGAGGCAG ATCCAGACTC CCACGGAAAG TAGACATGGG GAGCTGTGAG 
ATTTCCTCTA GTTCAGATAC TTTCAGCAGC CCCATACACT CTGCATCCAC TACAGGAGTG 
CTTGGCAGCC AGATTGACCA TAAAGAGGAT CACCAGTCCT CAAGTGGCAA CTGGAGTGGA 
AGCAGCTCCA CTTGCCCATC GCAGACATCC GAGACTCTTC CACCTGCTGC CTCCCCTCCC 
TTGACGGGAT CCTCACACTG TGATTCAGAA CTTTCCCTGA ATACTGGGTC CCATGTCATA 
GATGACAACA CTGGTTTCAT AATTGATCCC TATCACATAG ACAAGCCACA GGGACAGGGG 
CAACGATCCA ATTCATTTAC CTCATCAGCT ACTGATCAGA TGGATGATGC AGGGGTCAGC 
ACTGCTAGTG ATGGGGAGTG GAACTGTGCC CAGGACCAGC AGGATCAGTC CTGCACCCAA 
GACTTCAGCC CAAAGCGTTC CAGAGAGGAT GAGAGTGGTG TCAGCTGCCC TAGTTATTCT 
AGTGGGGAAA CTTACGAGAG CTTCAGTGAC CAAGCATCCG CTAGAAAATC TGAGATGCAC 
TACATTGTCG ACACTGAAGG ATTCTATTCC TCCATGCACT TTGACTCTGG TCTTAAGGGT 
AGCAGAAGCT ACTACAACTA TGCAGCCATT GACCCCGACT GTGGCCCAAG TGGTAATATA 
GCACCTGGCA TGACTGAATA CCCTCAGCCA GAGTACAGAG CCACCAGGAC ACTGGGCAGA 
CCCTGTCTCT CCTTAAGAAA ACCAAAGGCC AAGCCACCCC CACCAAAACG TAGCTCTTCA 
TTAAAGGAAA CAAGCAGTGA TGTGAACGTT CCAGAGCAGA ACGAACCAAA GATTACTTGT 
GAGTTGCCAC TGCCCTTGTC TTCCAGGGAG ATGAAACTGC AGCTGGACTT GGCTGATTCT 
GCAGGGCACC TTGAGACTGC AGGTCTTGAA TCACTTGGTG CATGGGGAGT GGAAAATGTT 
AGGGACATGC TTGACTGCTC CTCCCCATTC AGTTCCTCAG ACACACATTC CTTCAAAGAT 
GAAGGTGCTG TGCAAGCAGA CTATGCAGAC CTCTGGCTTC ACAATGACTT GAAATCAAAT 
GATCCTTATC GGTCCCTCTC TAACTCTAGC TCTGCTACAG GTACAACTGT TATTGAATGC 
ATCAAGTCAC CAGAAAGATC AGAAACGCAC ACCTGTCAAC CCAGGTCCAG ACCTTCTTCC 
CCAACTCTTC CTCCACCTGA AAGTGAATTT AAGCTTGCTT CTCCCGAAAA GCTGGCAGGC 
TTGGCGTCCC CTTCCAGTGG ATATTCCAGT CAGTCTGAAA CACCTACATC TTCCTTCCCC 
TCTGCTTTCT TCCCAGGGCC CCTGTCTCCA ACCAGTGGGA AGAGGAAGCC CAAAGTCCCT 
GAAAGGAAGT CCTCACTTTG TTCCTTACAG CAGCAACAGC TTTCAGTCAG AGACCCAGGC 
ATTTCCTGTA GGAGAGAAAC TGACTTCTAT GCCATACCCC CAAGTCACCT TGACCTAAGT 
GCTCTTCACA GTAAGCACTT TCCTCACAGA AATCAAATGC ACATCCTCCA CCAGAATAAG 
CAGAAAGCTG CCATAGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA 
GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AACTGCAGCC 
GCTGCTGCCG AGGCCTGCAC TGCAGCTACA GCTTCTACTA AAGAGAGTGC AACAAATACA 
GCACCCATCT CAGCCCATTT GGCTATTACT CCAACGGTTC TTAGATCAGT GCAACTGCGA 
TCTATATGTA GACCATCTGA TGGCAACCAA GGGCTTGATC AAGACAGTTC AAATCTCATA 
ACTCGTCCTA AGTGTCCCAC AATAATAACT GATGCCCCAT CATCTAGCAA CAGGCACAAC 
AGGAAGCCAC CAGCCTACAA ACCCCCTGCT GCACAGGTTT GTGATTCACA GATTCCACTG 
GTGGAAAACA TTGTTTTTCC ACAAGAAGAA GTGAGAGTGA GACAGGAGAG GTTTGGTCCT 
GGTACTTACT GGGCAATGAC TGCTTTCAGA ACTCCTGTGG ACCCTAATTG TGAAAAAGAA 
GTCTCTTTAA AAAGGTCATC TCAGTGTTTT CAGCAAGAAC AAGACAGCAG AAGGTGCCTA 
GATGTGCAAA AGACATTGTC ACCAGAGAGA CTAAAACAAG ATTTGAATCA ACTATCGCGG 
CCAGACCCTT CAACACAGTC CATGTGTTTG GCCAGCACAA TGCCACCCCC TGCTTATAGT 
GAGATACAGA GGAGCAATTT TGTACTGTGC AACAGTCCTG ACAAAGTGCC TGTTTCAACT 
CAAGAGGCAT CGCATTCTTA CACAATCAGC GATGTACAAG GCAGAGAGGA GGATTATGAG 
ACATCAGGTG TCGCAACCAG AAGTGCCTCA CAGGACATAA GGGAAGAAGA GTCAACCCCA 
GATACAGAGG ACTATTTCAG TAAAGACTCT ACTCCCAGTG ATAATTCATC CTCTCCTTTG 
ACCGATGACT CCAGACTTGA TGATGATGTT TTCCCATCTC CCAATAAACT CCGCACAACT 
GAAGATCTTT TTGCTATGAT ACACAGATCT AAAAGGAAAG TGCTGGGACG CAAAGATTCA 
GGGGAAGTCA GTGGAAAAGG TCGGCCTGTT GTACCACCTG TGACCACCCC TGTAAGCAAT 
CCTACTGTAT GCCCGGTCAT CCCTGCTCCC CCAGTTCCTT CAAACAACTC CCAGCGAGCC 
TCAGGACCCA TCTACAGGAG TGCCAAAAAG TCTAGTACAT CCAGTGAGGA GTTCAAACTC 
CTTCTGCTTA AGAAGGGTAG TCGCACAGAC TCAAGTTATC GCATGTCTGC TACAGAGATC 
CTTAAGAGCC CCATCACACC CAAGACTCAA GCAGAGCTGC TGTTAGAGGC CATGAGGCAA 
CCAGAGGAGA TCCCCTTACC CCAACTGGAT TCCACCAGCA GTGGAGATCC ACTTCCAAGT 
CCATTCCCTA AGGCCAACAG TGAGGGATTC TCCCCAAAAA CACTCACCAT GTCAGCTGCC 
TCCAGACAAG GACGTTCTAG AATTCCACCT GCAGCGAACA GCAGTCGCTA TAGCACGCGG 
AGTCGGCTGT ACACTGCCCC AATGCAGGCC ATATCAGAAG GAGAGACTGA GAACTCGGAT 
GGAAGTCCAC ACGATGACCG CTCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nhsb ( XM_009306120.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304395.1 Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X5, mRNA.

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CloneID	ODa43150
Clone ID Related Accession (Same CDS sequence)	XM_009306120.2 , XM_009306120.3
Accession Version	XM_009306120.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4830bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	Nance-Horan syndrome protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306120.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA 
GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT 
CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA 
CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC 
AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC 
CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG 
AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT 
GAATTCAAAC CTGTCCTCAG AAAGGCTGGA ACCAACACAG AAACGGATGG AGAACTACAA 
GTGATGAGCC ATAGGCCTAA ATGTCCTGTT CCCAATGCAC CTACCACCCT GGACAAACAG 
ACTAACTGGT CTAAAGCCCT GCCTCTTCCC ACTCCAGAAG AAAGAATAAA GAATGACTCT 
CAAGTGATTT CATCCTGTAT CATTCCAATA AATGTTTCGG GTGTTGGATT TGACAGAGAA 
GCCAGTGCTC GCTGCTCTCT TGTTCACTCG CAATCGGTTC TACAGAGACG GCGGAAACTC 
AGGAGGCGAA AAACGATCAC TGGGATCCCC AAACGAGTTC AACAAGACAT GGATTCTGAC 
GAATCCCCTG TTGCTAGAGA AAGAACAGTG ATAATCCACG CCAATCCACA CAAGTCCCAC 
GAGTGGCATG AGGAGCTCTC CTTGAGTGGC AGAGTATTGC ATACTAAGGA TTCGGGCTGT 
CAGACAGATG ACTTCTTGAT TACTGCTCCA TCCCGAAGAC GCATCCGTGC CCAAAGAGGT 
CAGGGAATTC CAGCGTCTCT CTCTCACTCC ACTGGAAACA TTACCTCCCT CCCGGATCGT 
TCTGATACGG TTTATGCTGC AGCATCAGCC ACTCGTGTCC GTTCTAGGAG CCTTCCACGT 
GAGGGTGGTC GGCTTCTGGA TGAGGACCAA GATGACAGTG ATGATGATGA TGATGAAGAT 
GATGATGAAG ATGATGAAGA GGAAGATGAG GAGCTCTCTC CATATGAAGC CGAAGACTTC 
CTGCCAGGTC CCGGACAGAG AATTCCAAAG GATGAGGAAG AGAGTACTGA CGACCAAGCC 
ATGCCAGAGC TACAGTTTGG AAGTCTTAAG CGTATGCAGC ATTCAGAGAG CCCTGACCAC 
ACATGGATTG AGAGAGGCAG ATCCAGACTC CCACGGAAAG TAGACATGGG GAGCTGTGAG 
ATTTCCTCTA GTTCAGATAC TTTCAGCAGC CCCATACACT CTGCATCCAC TACAGGAGTG 
CTTGGCAGCC AGATTGACCA TAAAGAGGAT CACCAGTCCT CAAGTGGCAA CTGGAGTGGA 
AGCAGCTCCA CTTGCCCATC GCAGACATCC GAGACTCTTC CACCTGCTGC CTCCCCTCCC 
TTGACGGGAT CCTCACACTG TGATTCAGAA CTTTCCCTGA ATACTGGGTC CCATGTCATA 
GATGACAACA CTGGTTTCAT AATTGATCCC TATCACATAG ACAAGCCACA GGGACAGGGG 
CAACGATCCA ATTCATTTAC CTCATCAGCT ACTGATCAGA TGGATGATGC AGGGGTCAGC 
ACTGCTAGTG ATGGGGAGTG GAACTGTGCC CAGGACCAGC AGGATCAGTC CTGCACCCAA 
GACTTCAGCC CAAAGCGTTC CAGAGAGGAT GAGAGTGGTG TCAGCTGCCC TAGTTATTCT 
AGTGGGGAAA CTTACGAGAG CTTCAGTGAC CAAGCATCCG CTAGAAAATC TGAGATGCAC 
TACATTGTCG ACACTGAAGG ATTCTATTCC TCCATGCACT TTGACTCTGG TCTTAAGGGT 
AGCAGAAGCT ACTACAACTA TGCAGCCATT GACCCCGACT GTGGCCCAAG TGGTAATATA 
GCACCTGGCA TGACTGAATA CCCTCAGCCA GAGTACAGAG CCACCAGGAC ACTGGGCAGA 
CCCTGTCTCT CCTTAAGAAA ACCAAAGGCC AAGCCACCCC CACCAAAACG TAGCTCTTCA 
TTAAAGGAAA CAAGCAGTGA TGTGAACGTT CCAGAGCAGA ACGAACCAAA GATTACTTGT 
GAGTTGCCAC TGCCCTTGTC TTCCAGGGAG ATGAAACTGC AGCTGGACTT GGCTGATTCT 
GCAGGGCACC TTGAGACTGC AGGTCTTGAA TCACTTGGTG CATGGGGAGT GGAAAATGTT 
AGGGACATGC TTGACTGCTC CTCCCCATTC AGTTCCTCAG ACACACATTC CTTCAAAGAT 
GAAGGTGCTG TGCAAGCAGA CTATGCAGAC CTCTGGCTTC ACAATGACTT GAAATCAAAT 
GATCCTTATC GGTCCCTCTC TAACTCTAGC TCTGCTACAG GTACAACTGT TATTGAATGC 
ATCAAGTCAC CAGAAAGATC AGAAACGCAC ACCTGTCAAC CCAGGTCCAG ACCTTCTTCC 
CCAACTCTTC CTCCACCTGA AAGTGAATTT AAGCTTGCTT CTCCCGAAAA GCTGGCAGGC 
TTGGCGTCCC CTTCCAGTGG ATATTCCAGT CAGTCTGAAA CACCTACATC TTCCTTCCCC 
TCTGCTTTCT TCCCAGGGCC CCTGTCTCCA ACCAGTGGGA AGAGGAAGCC CAAAGTCCCT 
GAAAGGAAGT CCTCACTTTG TTCCTTACAG CAGCAACAGC TTTCAGTCAG AGACCCAGGC 
ATTTCCTGTA GGAGAGAAAC TGACTTCTAT GCCATACCCC CAAGTCACCT TGACCTAAGT 
GCTCTTCACA GTAAGCACTT TCCTCACAGA AATCAAATGC ACATCCTCCA CCAGAATAAG 
CAGAAAGCTG CCATAGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA 
GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AACTGCAGCC 
GCTGCTGCCG AGGCCTGCAC TGCAGCTACA GCTTCTACTA AAGAGAGTGC AACAAATACA 
GCACCCATCT CAGCCCATTT GGCTATTACT CCAACGGTTC TTAGATCAGT GCAACTGCGA 
TCTATATGTA GACCATCTGA TGGCAACCAA GGGCTTGATC AAGACAGTTC AAATCTCATA 
ACTCGTCCTA AGTGTCCCAC AATAATAACT GATGCCCCAT CATCTAGCAA CAGGCACAAC 
AGGAAGCCAC CAGCCTACAA ACCCCCTGCT GCACAGGTTT GTGATTCACA GATTCCACTG 
GTGGAAAACA TTGTTTTTCC ACAAGAAGAA GTGAGAGTGA GACAGGAGAG GTTTGGTCCT 
GGTACTTACT GGGCAATGAC TGCTTTCAGA ACTCCTGTGG ACCCTAATTG TGAAAAAGAA 
GTCTCTTTAA AAAGGTCATC TCAGTGTTTT CAGCAAGAAC AAGACAGCAG AAGGTGCCTA 
GATGTGCAAA AGACATTGTC ACCAGAGAGA CTAAAACAAG ATTTGAATCA ACTATCGCGG 
CCAGACCCTT CAACACAGTC CATGTGTTTG GCCAGCACAA TGCCACCCCC TGCTTATAGT 
GAGATACAGA GGAGCAATTT TGTACTGTGC AACAGTCCTG ACAAAGTGCC TGTTTCAACT 
CAAGAGGCAT CGCATTCTTA CACAATCAGC GATGTACAAG GCAGAGAGGA GGATTATGAG 
ACATCAGGTG TCGCAACCAG AAGTGCCTCA CAGGACATAA GGGAAGAAGA GTCAACCCCA 
GATACAGAGG ACTATTTCAG TAAAGACTCT ACTCCCAGTG ATAATTCATC CTCTCCTTTG 
ACCGATGACT CCAGACTTGA TGATGATGTT TTCCCATCTC CCAATAAACT CCGCACAACT 
GAAGATCTTT TTGCTATGAT ACACAGATCT AAAAGGAAAG TGCTGGGACG CAAAGATTCA 
GGGGAAGTCA GTGGAAAAGG TCGGCCTGTT GTACCACCTG TGACCACCCC TGTAAGCAAT 
CCTACTGTAT GCCCGGTCAT CCCTGCTCCC CCAGTTCCTT CAAACAACTC CCAGCGAGCC 
TCAGGACCCA TCTACAGGAG TGCCAAAAAG TCTAGTACAT CCAGTGAGGA GTTCAAACTC 
CTTCTGCTTA AGAAGGGTAG TCGCACAGAC TCAAGTTATC GCATGTCTGC TACAGAGATC 
CTTAAGAGCC CCATCACACC CAAGACTCAA GCAGAGCTGC TGTTAGAGGC CATGAGGCAA 
CCAGAGGAGA TCCCCTTACC CCAACTGGAT TCCACCAGCA GTGGAGATCC ACTTCCAAGT 
CCATTCCCTA AGGCCAACAG TGAGGGATTC TCCCCAAAAA CACTCACCAT GTCAGCTGCC 
TCCAGACAAG GACGTTCTAG AATTCCACCT GCAGCGAACA GCAGTCGCTA TAGCACGCGG 
AGTCGGCTGT ACACTGCCCC AATGCAGGCC ATATCAGAAG GAGAGACTGA GAACTCGGAT 
GGAAGTCCAC ACGATGACCG CTCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304395.1
CDS	320..5149
Translation	MPFAKRIVEPQLLVRHPIPNDEGLLFEDLCSITNVALSRTLRQLSDLSKHACSLFQELENEIVSTNQRVWALQNKIGKIQQTASALDPKMEAVRFRSISIAVSNLDIESKLTSHYQAPWHQQHNLFHTCTRPACLEELHRHAKLNLRALHRDQQQRQRSTSRERSRVTISISVAPPMPTFPSTHKLRRREQRGRHSRMERSVRESDVQTIQRKERPAKDSEFKPVLRKAGTNTETDGELQVMSHRPKCPVPNAPTTLDKQTNWSKALPLPTPEERIKNDSQVISSCIIPINVSGVGFDREASARCSLVHSQSVLQRRRKLRRRKTITGIPKRVQQDMDSDESPVARERTVIIHANPHKSHEWHEELSLSGRVLHTKDSGCQTDDFLITAPSRRRIRAQRGQGIPASLSHSTGNITSLPDRSDTVYAAASATRVRSRSLPREGGRLLDEDQDDSDDDDDEDDDEDDEEEDEELSPYEAEDFLPGPGQRIPKDEEESTDDQAMPELQFGSLKRMQHSESPDHTWIERGRSRLPRKVDMGSCEISSSSDTFSSPIHSASTTGVLGSQIDHKEDHQSSSGNWSGSSSTCPSQTSETLPPAASPPLTGSSHCDSELSLNTGSHVIDDNTGFIIDPYHIDKPQGQGQRSNSFTSSATDQMDDAGVSTASDGEWNCAQDQQDQSCTQDFSPKRSREDESGVSCPSYSSGETYESFSDQASARKSEMHYIVDTEGFYSSMHFDSGLKGSRSYYNYAAIDPDCGPSGNIAPGMTEYPQPEYRATRTLGRPCLSLRKPKAKPPPPKRSSSLKETSSDVNVPEQNEPKITCELPLPLSSREMKLQLDLADSAGHLETAGLESLGAWGVENVRDMLDCSSPFSSSDTHSFKDEGAVQADYADLWLHNDLKSNDPYRSLSNSSSATGTTVIECIKSPERSETHTCQPRSRPSSPTLPPPESEFKLASPEKLAGLASPSSGYSSQSETPTSSFPSAFFPGPLSPTSGKRKPKVPERKSSLCSLQQQQLSVRDPGISCRRETDFYAIPPSHLDLSALHSKHFPHRNQMHILHQNKQKAAIAAAAAAEARSAAAAAAEARSAAAAAAEARSAATAAAAAEACTAATASTKESATNTAPISAHLAITPTVLRSVQLRSICRPSDGNQGLDQDSSNLITRPKCPTIITDAPSSSNRHNRKPPAYKPPAAQVCDSQIPLVENIVFPQEEVRVRQERFGPGTYWAMTAFRTPVDPNCEKEVSLKRSSQCFQQEQDSRRCLDVQKTLSPERLKQDLNQLSRPDPSTQSMCLASTMPPPAYSEIQRSNFVLCNSPDKVPVSTQEASHSYTISDVQGREEDYETSGVATRSASQDIREEESTPDTEDYFSKDSTPSDNSSSPLTDDSRLDDDVFPSPNKLRTTEDLFAMIHRSKRKVLGRKDSGEVSGKGRPVVPPVTTPVSNPTVCPVIPAPPVPSNNSQRASGPIYRSAKKSSTSSEEFKLLLLKKGSRTDSSYRMSATEILKSPITPKTQAELLLEAMRQPEEIPLPQLDSTSSGDPLPSPFPKANSEGFSPKTLTMSAASRQGRSRIPPAANSSRYSTRSRLYTAPMQAISEGETENSDGSPHDDRSS

Target ORF information:

RefSeq Version	XM_009306120.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Nance-Horan syndrome b (congenital cataracts and dental anomalies) (nhsb), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306120.3

ORF Insert Sequence:

ATGCCTTTTG CCAAACGGAT CGTTGAGCCC CAGCTGCTGG TTAGACATCC CATCCCTAAT 
GATGAAGGGT TACTGTTCGA GGATCTGTGC TCCATCACCA ATGTGGCTCT ATCCCGGACC 
CTGCGGCAGC TCTCCGACCT GTCCAAACAC GCTTGCTCCT TATTTCAGGA GCTTGAGAAC 
GAAATAGTGT CCACAAACCA ACGGGTTTGG GCTTTGCAGA ACAAAATAGG CAAAATTCAG 
CAAACCGCCA GTGCGCTGGA TCCAAAAATG GAGGCTGTGC GTTTCAGGAG CATCTCTATA 
GCGGTCTCGA ATCTGGACAT TGAGAGTAAA CTGACGTCGC ACTATCAGGC TCCATGGCAT 
CAGCAACACA ATCTGTTCCA CACTTGCACA CGGCCAGCGT GTCTGGAGGA GCTGCACCGA 
CATGCCAAGC TCAACCTGCG GGCGCTGCAC AGAGACCAGC AGCAGCGTCA GCGTTCAACC 
AGCAGAGAGC GCAGTCGGGT CACTATATCA ATATCTGTGG CACCGCCAAT GCCAACCTTC 
CCTTCAACTC ACAAACTCAG ACGGCGAGAG CAGAGAGGTC GACATTCACG CATGGAGAGG 
AGCGTTAGAG AGAGTGATGT GCAAACTATC CAGAGGAAGG AGAGACCAGC CAAGGATTCT 
GAATTCAAAC CTGTCCTCAG AAAGGCTGGA ACCAACACAG AAACGGATGG AGAACTACAA 
GTGATGAGCC ATAGGCCTAA ATGTCCTGTT CCCAATGCAC CTACCACCCT GGACAAACAG 
ACTAACTGGT CTAAAGCCCT GCCTCTTCCC ACTCCAGAAG AAAGAATAAA GAATGACTCT 
CAAGTGATTT CATCCTGTAT CATTCCAATA AATGTTTCGG GTGTTGGATT TGACAGAGAA 
GCCAGTGCTC GCTGCTCTCT TGTTCACTCG CAATCGGTTC TACAGAGACG GCGGAAACTC 
AGGAGGCGAA AAACGATCAC TGGGATCCCC AAACGAGTTC AACAAGACAT GGATTCTGAC 
GAATCCCCTG TTGCTAGAGA AAGAACAGTG ATAATCCACG CCAATCCACA CAAGTCCCAC 
GAGTGGCATG AGGAGCTCTC CTTGAGTGGC AGAGTATTGC ATACTAAGGA TTCGGGCTGT 
CAGACAGATG ACTTCTTGAT TACTGCTCCA TCCCGAAGAC GCATCCGTGC CCAAAGAGGT 
CAGGGAATTC CAGCGTCTCT CTCTCACTCC ACTGGAAACA TTACCTCCCT CCCGGATCGT 
TCTGATACGG TTTATGCTGC AGCATCAGCC ACTCGTGTCC GTTCTAGGAG CCTTCCACGT 
GAGGGTGGTC GGCTTCTGGA TGAGGACCAA GATGACAGTG ATGATGATGA TGATGAAGAT 
GATGATGAAG ATGATGAAGA GGAAGATGAG GAGCTCTCTC CATATGAAGC CGAAGACTTC 
CTGCCAGGTC CCGGACAGAG AATTCCAAAG GATGAGGAAG AGAGTACTGA CGACCAAGCC 
ATGCCAGAGC TACAGTTTGG AAGTCTTAAG CGTATGCAGC ATTCAGAGAG CCCTGACCAC 
ACATGGATTG AGAGAGGCAG ATCCAGACTC CCACGGAAAG TAGACATGGG GAGCTGTGAG 
ATTTCCTCTA GTTCAGATAC TTTCAGCAGC CCCATACACT CTGCATCCAC TACAGGAGTG 
CTTGGCAGCC AGATTGACCA TAAAGAGGAT CACCAGTCCT CAAGTGGCAA CTGGAGTGGA 
AGCAGCTCCA CTTGCCCATC GCAGACATCC GAGACTCTTC CACCTGCTGC CTCCCCTCCC 
TTGACGGGAT CCTCACACTG TGATTCAGAA CTTTCCCTGA ATACTGGGTC CCATGTCATA 
GATGACAACA CTGGTTTCAT AATTGATCCC TATCACATAG ACAAGCCACA GGGACAGGGG 
CAACGATCCA ATTCATTTAC CTCATCAGCT ACTGATCAGA TGGATGATGC AGGGGTCAGC 
ACTGCTAGTG ATGGGGAGTG GAACTGTGCC CAGGACCAGC AGGATCAGTC CTGCACCCAA 
GACTTCAGCC CAAAGCGTTC CAGAGAGGAT GAGAGTGGTG TCAGCTGCCC TAGTTATTCT 
AGTGGGGAAA CTTACGAGAG CTTCAGTGAC CAAGCATCCG CTAGAAAATC TGAGATGCAC 
TACATTGTCG ACACTGAAGG ATTCTATTCC TCCATGCACT TTGACTCTGG TCTTAAGGGT 
AGCAGAAGCT ACTACAACTA TGCAGCCATT GACCCCGACT GTGGCCCAAG TGGTAATATA 
GCACCTGGCA TGACTGAATA CCCTCAGCCA GAGTACAGAG CCACCAGGAC ACTGGGCAGA 
CCCTGTCTCT CCTTAAGAAA ACCAAAGGCC AAGCCACCCC CACCAAAACG TAGCTCTTCA 
TTAAAGGAAA CAAGCAGTGA TGTGAACGTT CCAGAGCAGA ACGAACCAAA GATTACTTGT 
GAGTTGCCAC TGCCCTTGTC TTCCAGGGAG ATGAAACTGC AGCTGGACTT GGCTGATTCT 
GCAGGGCACC TTGAGACTGC AGGTCTTGAA TCACTTGGTG CATGGGGAGT GGAAAATGTT 
AGGGACATGC TTGACTGCTC CTCCCCATTC AGTTCCTCAG ACACACATTC CTTCAAAGAT 
GAAGGTGCTG TGCAAGCAGA CTATGCAGAC CTCTGGCTTC ACAATGACTT GAAATCAAAT 
GATCCTTATC GGTCCCTCTC TAACTCTAGC TCTGCTACAG GTACAACTGT TATTGAATGC 
ATCAAGTCAC CAGAAAGATC AGAAACGCAC ACCTGTCAAC CCAGGTCCAG ACCTTCTTCC 
CCAACTCTTC CTCCACCTGA AAGTGAATTT AAGCTTGCTT CTCCCGAAAA GCTGGCAGGC 
TTGGCGTCCC CTTCCAGTGG ATATTCCAGT CAGTCTGAAA CACCTACATC TTCCTTCCCC 
TCTGCTTTCT TCCCAGGGCC CCTGTCTCCA ACCAGTGGGA AGAGGAAGCC CAAAGTCCCT 
GAAAGGAAGT CCTCACTTTG TTCCTTACAG CAGCAACAGC TTTCAGTCAG AGACCCAGGC 
ATTTCCTGTA GGAGAGAAAC TGACTTCTAT GCCATACCCC CAAGTCACCT TGACCTAAGT 
GCTCTTCACA GTAAGCACTT TCCTCACAGA AATCAAATGC ACATCCTCCA CCAGAATAAG 
CAGAAAGCTG CCATAGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA 
GCAGAAGCTC GCAGTGCAGC AGCTGCTGCA GCAGAAGCTC GCAGTGCAGC AACTGCAGCC 
GCTGCTGCCG AGGCCTGCAC TGCAGCTACA GCTTCTACTA AAGAGAGTGC AACAAATACA 
GCACCCATCT CAGCCCATTT GGCTATTACT CCAACGGTTC TTAGATCAGT GCAACTGCGA 
TCTATATGTA GACCATCTGA TGGCAACCAA GGGCTTGATC AAGACAGTTC AAATCTCATA 
ACTCGTCCTA AGTGTCCCAC AATAATAACT GATGCCCCAT CATCTAGCAA CAGGCACAAC 
AGGAAGCCAC CAGCCTACAA ACCCCCTGCT GCACAGGTTT GTGATTCACA GATTCCACTG 
GTGGAAAACA TTGTTTTTCC ACAAGAAGAA GTGAGAGTGA GACAGGAGAG GTTTGGTCCT 
GGTACTTACT GGGCAATGAC TGCTTTCAGA ACTCCTGTGG ACCCTAATTG TGAAAAAGAA 
GTCTCTTTAA AAAGGTCATC TCAGTGTTTT CAGCAAGAAC AAGACAGCAG AAGGTGCCTA 
GATGTGCAAA AGACATTGTC ACCAGAGAGA CTAAAACAAG ATTTGAATCA ACTATCGCGG 
CCAGACCCTT CAACACAGTC CATGTGTTTG GCCAGCACAA TGCCACCCCC TGCTTATAGT 
GAGATACAGA GGAGCAATTT TGTACTGTGC AACAGTCCTG ACAAAGTGCC TGTTTCAACT 
CAAGAGGCAT CGCATTCTTA CACAATCAGC GATGTACAAG GCAGAGAGGA GGATTATGAG 
ACATCAGGTG TCGCAACCAG AAGTGCCTCA CAGGACATAA GGGAAGAAGA GTCAACCCCA 
GATACAGAGG ACTATTTCAG TAAAGACTCT ACTCCCAGTG ATAATTCATC CTCTCCTTTG 
ACCGATGACT CCAGACTTGA TGATGATGTT TTCCCATCTC CCAATAAACT CCGCACAACT 
GAAGATCTTT TTGCTATGAT ACACAGATCT AAAAGGAAAG TGCTGGGACG CAAAGATTCA 
GGGGAAGTCA GTGGAAAAGG TCGGCCTGTT GTACCACCTG TGACCACCCC TGTAAGCAAT 
CCTACTGTAT GCCCGGTCAT CCCTGCTCCC CCAGTTCCTT CAAACAACTC CCAGCGAGCC 
TCAGGACCCA TCTACAGGAG TGCCAAAAAG TCTAGTACAT CCAGTGAGGA GTTCAAACTC 
CTTCTGCTTA AGAAGGGTAG TCGCACAGAC TCAAGTTATC GCATGTCTGC TACAGAGATC 
CTTAAGAGCC CCATCACACC CAAGACTCAA GCAGAGCTGC TGTTAGAGGC CATGAGGCAA 
CCAGAGGAGA TCCCCTTACC CCAACTGGAT TCCACCAGCA GTGGAGATCC ACTTCCAAGT 
CCATTCCCTA AGGCCAACAG TGAGGGATTC TCCCCAAAAA CACTCACCAT GTCAGCTGCC 
TCCAGACAAG GACGTTCTAG AATTCCACCT GCAGCGAACA GCAGTCGCTA TAGCACGCGG 
AGTCGGCTGT ACACTGCCCC AATGCAGGCC ATATCAGAAG GAGAGACTGA GAACTCGGAT 
GGAAGTCCAC ACGATGACCG CTCCTCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	nhsb
Entrez Gene ID	559636
Full Name	Nance-Horan syndrome b (congenital cataracts and dental anomalies)
Synonyms	nhs
General protein information	Preferred Names Nance-Horan syndrome b (congenital cataracts and dental anomalies) Names Nance-Horan syndrome protein Nance-Horan syndrome (congenital cataracts and dental anomalies) nance-Horan syndrome protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Canis lupus familiaris (dog)	NHS	XP_548877.2
Bos taurus (cattle)	NHS	NP_001192657.1
Mus musculus (house mouse)	Nhs	NP_001074521.1
	NHS	XP_001104229.2
Rattus norvegicus (Norway rat)	Nhs	NP_001178662.1
Danio rerio (zebrafish)	nhs	XP_005169435.1
Gallus gallus (chicken)	NHS	XP_416815.4
Danio rerio (zebrafish)	nhsa	XP_001919340.4
Xenopus tropicalis (tropical clawed frog)	LOC100498628	XP_002934404.2
Homo sapiens (human)	NHS	NP_938011.1
Pan troglodytes (chimpanzee)	NHS	XP_520958.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

nhsb cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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