ANKRD27 cDNA ORF clone, Canis lupus familiaris(dog)

The following ANKRD27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKRD27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe285770 XM_022407371.1
Latest version!
Canis lupus familiaris ankyrin repeat domain 27 (ANKRD27), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe34769 XM_014116765.1
Latest version!
Canis lupus familiaris ankyrin repeat domain 27 (VPS9 domain) (ANKRD27), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe285770
    Clone ID Related Accession (Same CDS sequence) XM_022407371.1
    Accession Version XM_022407371.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3366bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product ankyrin repeat domain-containing protein 27
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006583.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATAGATA CCGGACCTAG AAAGGATTCA GACCCAAAAC CAGCCCCTCC ACGTGCGAGA 
    GACACTGAGA AGTGGCCTTT GGAGCTCCTG CCCTTCTGGT CGAGATTCTG GCAAGAAAGT
    GGGTGCTGGT GGCACTGCCT GCAACTCCAG GAGCAGGGTT GCTACCTCCT GCATCCGAGC
    CCACCCTTGC CACCTCTGTC TCGGGTGCAG AGATGTGGGA GCTCCGCTGG CATCTCGTCT
    GAGGAGCAGA AGGAGCATCG CTTCCCAGCT TCATTCCGAC CGCGTGGCAT CCTGCCTCTC
    ATGCGGTTTC TGTCCAAGGA GACTCTCTCT GCCCCATCTG AAGTCCATAT GGCTCTGTAT
    GATGAAGATC TCCTGAAAAA TCCTTTCTAT CTGGCTCTTC AAAAGTGGCG TCCTGACTTA
    TGCAGCAAGG TGGCCCAGAT CCATGGCATC GTCCTGGTGC CTTGCAAAGG AAGCTTGTCG
    AGCAGTGTTC AGTCCACTTG TCAGTTTGAA TCTTATATTT TGATACCCGT GGAAGAACAC
    TTTCAGACCT TGAATGGAAA GGATGTCTTT ATACAGGGAA ACAGGATTAA ATTAGGAGCT
    GGTTTCGCCT GTCTTCTCTC AGTGCCCATT CTCTTTGAAG AAACTTTCTA CAATGAAAAA
    GAAGAAAGTT TCAGCATACT GTGTATAGCC CATCCTTTGG AAAAGAGAGA GAGTTCAGAA
    GAGCCTTCAA CATCTTCCGA TCCCTTTTCC CTGAAAACCA TCGAAGACGT GAGAGAGTTT
    TTGGGAAGAC ACGCGGAGAG ATTTGACAGG AGCATTGCCT CTTTCCAGCG AACCTTCAGG
    GAATGTGAAA GAAAGAGCCT CCGTCAGCAC ATAGACTCGG TGAATGCTCT CTACACCAAA
    TGCCTCCAGC AGCTTCTGAG GGACTCTCAC CTGAAAATGC TTGCCAAGCA GGAGGCCCAG
    ATGAATCTGC TGAAGCAGGC CGTGGAGATG TATGTCCAGC ATGATATTTA TGATCTGATC
    TTTAAGTACG TGGGGACCAT GGAGGCGAGC GAGGATGCTG CCTTTAACAA AATCACAAGA
    AGCCTTCAAG ATCTTCAGCA GAAAGACATT GGTGTGAAAC CCGAGTGCAG CTTCAACATA
    CCTCGTGCAA AGAGAGAGCT GGCTCAGCTG AACAAATGCA CCTCACCACA GCAGAAGTTG
    ATTTGTTTGC GAAAGGTGGT GCAGCTCATC ACACAGTCCC CCAGCCAGAG AGTTAACTTG
    GAGACCATGT GTGCTGATGA TCTGCTGTCG GTCCTGTTGT ATTTGCTTGT GAAAACAGAT
    ATCCCTAATT GGATGGCAAA TTTGAGTTAT ATCAAGAACT TCAGATTTAG CAGCTCAGCA
    AAAGATGAAT TGGGATATTG TCTGACCTCG ATTGAGGCTG CGATTGAATA TATTCGGCAA
    GGAAGCCTCT CCGTGAAGCC ACCCGAGTCG GAGGGGTTTG GTGACAGACT GTTCCTGAAG
    CAGAGAATGA GCTTACTGTC TCAGATGACT TCAACTCCCA TTGATTGTCT GTTTAAGCAC
    ATCGCGTCAG GTAACCAGAA AGAAGTGGAA AGACTCCTGA GCCAAGATGA CCAGGATAAA
    GATGCCGTCC AAAGGATGTG TCACCCTCTG TGCTTCTGTG ACGACTGTGA GAAACTTGTC
    TCTGGGAGGC TGAACGATCC CTCCATTGTC ACTCCATTTT CCAGAGATGA CAGGGGTCAC
    ACACCGCTCC ATGTGGCCGC CCTCTGTGGG CAGGCATCCC TCATTGACTT CCTGGTTTCC
    AGAGGCGCGG GGGTAAATGC CACGGACTAC CACGGGTCGG CCCCCCTTCA CCTCGCCTGC
    CAGAAGGGCT ATCAGAGCGT CACGGTGAGC CCGGCTCCGC TGCAGCCTCG CGCGGGGTTG
    GGGGGTGCGT GTCTGAGCCC CTGGCACCTT CCGTGGGCCC CCTGGACCCA GACCACTCAC
    TGGCACTTCG GGGATGGAGG CTCGTTAGGG ATCTCTCACG CCCCCTTCTT GTCCCAGATT
    CTGTCAATAA TGGAAGCCCA TCACCTGCCC TTGGAAAGGA GGCGGAAGTC TTCAGAGGTC
    CCCGTGCAGC CCCCTCAGCG CCCCGCGGAT TCCATCAGCC AGGCCTCCTC CACCTCCAGC
    TTCTCCTCCG TGTTGGCGAG CTCCAGGCAG GAGGAGCCCA AGAAGGACTA CAGAGAGGTA
    GAAAAACTTT TAAGAGCGGT TGCTGACGGG GATCTAGAAA TGGTGCGTTA CCTTTTGGAG
    TGGACCGAGG AGGACCTGGA TGAATTGGAG GACGCCGCCG CTGTGGGTGA TGTCGACTTC
    TGTCACCCCC TGTGCCAGTG TCCGAGATGC GCTCCGGCTC AGAAGAAGCT GGCAAAGATT
    CCTGCCAGCG GGCTTGGTGT AAATGTGACC AACCAGGACG GCACCTCCCC GCTGCATGTT
    GCTGCCCTGC ACGGTCGGGC GGACCTCATC CCCCTCCTGT TGAAACACGG TGCCAATGCC
    GGTGCCAGAA ACGTAAACCA AGCTGTCCCG CTCCACCTGG CCTGCCAGAA GGGCCACTTT
    CAGGTGGTGA GGTGTCTGTT GGATTCTAAA GCAAAACCCA ATAAAAAGGA TATAAGTGGA
    AACACACCCC TCATTTACGC CTGTTCCAAC GGCTACCACG AAGTTGCAGC ATTGCTGTTA
    CAGCACGGGG CCTCCATCAA CATCTGTAAC AATAAGGGTA ACACAGCGCT GCACGAGGCG
    GTGATAGAAA AGCACGTCTT CGTGGTGGAG CTGCTTCTGC TTCATGGGGC ATCAGTTCAG
    GTGGTGAACA AGCGTCAGTG CACCGCCATC GATTGTGCTG AACAGAACTC AAAAATAATG
    GAATTACTTC AAGTGGTACC GAGCTGTGTG GCGTCCGTGG ACGACGTGGG CCAAACAGAC
    CGCAAGGAGT ACGTGACGGT TAAGATCAGG AAAAAATGGA ACTCAAAAAT GTATGACCTG
    CCAGATGAAC CTTTTACCAG ACAGTTTTAC TTTGTCCCCT CAGGTGGTCA GTTTAAGGGA
    AGGACTTCAA GGGAGATTCT GGCAAGAGAT AGAAGTGTCC CTAATTTTAC TGAAGACTCT
    CTGCATGAGC CAGGGAGGCA AACAGTCACA GGCAGACAGA GCAACCTGCC AGACCAGAGC
    AGATCTGAGA CTGCTGACCG AGGAGGCGGC AATCGGCCCG AGAGGCCTGG ACCGAAACAA
    AGCGTTCCTG CCAACAGGCG GATGCTGCGC AGACACACAG TCGAGGACCC AGTCGTATCC
    GTGAGCCCAG GCTCCGCCGG CAACCCAAGC ACATCCCCAG AGGCCGATAC CGCTTCCCAG
    TCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022263079.1
    CDS196..3561
    Translation

    Target ORF information:

    RefSeq Version XM_022407371.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris ankyrin repeat domain 27 (ANKRD27), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022407371.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGATAGATA CCGGACCTAG AAAGGATTCA GACCCAAAAC CAGCCCCTCC ACGTGCGAGA 
    GACACTGAGA AGTGGCCTTT GGAGCTCCTG CCCTTCTGGT CGAGATTCTG GCAAGAAAGT
    GGGTGCTGGT GGCACTGCCT GCAACTCCAG GAGCAGGGTT GCTACCTCCT GCATCCGAGC
    CCACCCTTGC CACCTCTGTC TCGGGTGCAG AGATGTGGGA GCTCCGCTGG CATCTCGTCT
    GAGGAGCAGA AGGAGCATCG CTTCCCAGCT TCATTCCGAC CGCGTGGCAT CCTGCCTCTC
    ATGCGGTTTC TGTCCAAGGA GACTCTCTCT GCCCCATCTG AAGTCCATAT GGCTCTGTAT
    GATGAAGATC TCCTGAAAAA TCCTTTCTAT CTGGCTCTTC AAAAGTGGCG TCCTGACTTA
    TGCAGCAAGG TGGCCCAGAT CCATGGCATC GTCCTGGTGC CTTGCAAAGG AAGCTTGTCG
    AGCAGTGTTC AGTCCACTTG TCAGTTTGAA TCTTATATTT TGATACCCGT GGAAGAACAC
    TTTCAGACCT TGAATGGAAA GGATGTCTTT ATACAGGGAA ACAGGATTAA ATTAGGAGCT
    GGTTTCGCCT GTCTTCTCTC AGTGCCCATT CTCTTTGAAG AAACTTTCTA CAATGAAAAA
    GAAGAAAGTT TCAGCATACT GTGTATAGCC CATCCTTTGG AAAAGAGAGA GAGTTCAGAA
    GAGCCTTCAA CATCTTCCGA TCCCTTTTCC CTGAAAACCA TCGAAGACGT GAGAGAGTTT
    TTGGGAAGAC ACGCGGAGAG ATTTGACAGG AGCATTGCCT CTTTCCAGCG AACCTTCAGG
    GAATGTGAAA GAAAGAGCCT CCGTCAGCAC ATAGACTCGG TGAATGCTCT CTACACCAAA
    TGCCTCCAGC AGCTTCTGAG GGACTCTCAC CTGAAAATGC TTGCCAAGCA GGAGGCCCAG
    ATGAATCTGC TGAAGCAGGC CGTGGAGATG TATGTCCAGC ATGATATTTA TGATCTGATC
    TTTAAGTACG TGGGGACCAT GGAGGCGAGC GAGGATGCTG CCTTTAACAA AATCACAAGA
    AGCCTTCAAG ATCTTCAGCA GAAAGACATT GGTGTGAAAC CCGAGTGCAG CTTCAACATA
    CCTCGTGCAA AGAGAGAGCT GGCTCAGCTG AACAAATGCA CCTCACCACA GCAGAAGTTG
    ATTTGTTTGC GAAAGGTGGT GCAGCTCATC ACACAGTCCC CCAGCCAGAG AGTTAACTTG
    GAGACCATGT GTGCTGATGA TCTGCTGTCG GTCCTGTTGT ATTTGCTTGT GAAAACAGAT
    ATCCCTAATT GGATGGCAAA TTTGAGTTAT ATCAAGAACT TCAGATTTAG CAGCTCAGCA
    AAAGATGAAT TGGGATATTG TCTGACCTCG ATTGAGGCTG CGATTGAATA TATTCGGCAA
    GGAAGCCTCT CCGTGAAGCC ACCCGAGTCG GAGGGGTTTG GTGACAGACT GTTCCTGAAG
    CAGAGAATGA GCTTACTGTC TCAGATGACT TCAACTCCCA TTGATTGTCT GTTTAAGCAC
    ATCGCGTCAG GTAACCAGAA AGAAGTGGAA AGACTCCTGA GCCAAGATGA CCAGGATAAA
    GATGCCGTCC AAAGGATGTG TCACCCTCTG TGCTTCTGTG ACGACTGTGA GAAACTTGTC
    TCTGGGAGGC TGAACGATCC CTCCATTGTC ACTCCATTTT CCAGAGATGA CAGGGGTCAC
    ACACCGCTCC ATGTGGCCGC CCTCTGTGGG CAGGCATCCC TCATTGACTT CCTGGTTTCC
    AGAGGCGCGG GGGTAAATGC CACGGACTAC CACGGGTCGG CCCCCCTTCA CCTCGCCTGC
    CAGAAGGGCT ATCAGAGCGT CACGGTGAGC CCGGCTCCGC TGCAGCCTCG CGCGGGGTTG
    GGGGGTGCGT GTCTGAGCCC CTGGCACCTT CCGTGGGCCC CCTGGACCCA GACCACTCAC
    TGGCACTTCG GGGATGGAGG CTCGTTAGGG ATCTCTCACG CCCCCTTCTT GTCCCAGATT
    CTGTCAATAA TGGAAGCCCA TCACCTGCCC TTGGAAAGGA GGCGGAAGTC TTCAGAGGTC
    CCCGTGCAGC CCCCTCAGCG CCCCGCGGAT TCCATCAGCC AGGCCTCCTC CACCTCCAGC
    TTCTCCTCCG TGTTGGCGAG CTCCAGGCAG GAGGAGCCCA AGAAGGACTA CAGAGAGGTA
    GAAAAACTTT TAAGAGCGGT TGCTGACGGG GATCTAGAAA TGGTGCGTTA CCTTTTGGAG
    TGGACCGAGG AGGACCTGGA TGAATTGGAG GACGCCGCCG CTGTGGGTGA TGTCGACTTC
    TGTCACCCCC TGTGCCAGTG TCCGAGATGC GCTCCGGCTC AGAAGAAGCT GGCAAAGATT
    CCTGCCAGCG GGCTTGGTGT AAATGTGACC AACCAGGACG GCACCTCCCC GCTGCATGTT
    GCTGCCCTGC ACGGTCGGGC GGACCTCATC CCCCTCCTGT TGAAACACGG TGCCAATGCC
    GGTGCCAGAA ACGTAAACCA AGCTGTCCCG CTCCACCTGG CCTGCCAGAA GGGCCACTTT
    CAGGTGGTGA GGTGTCTGTT GGATTCTAAA GCAAAACCCA ATAAAAAGGA TATAAGTGGA
    AACACACCCC TCATTTACGC CTGTTCCAAC GGCTACCACG AAGTTGCAGC ATTGCTGTTA
    CAGCACGGGG CCTCCATCAA CATCTGTAAC AATAAGGGTA ACACAGCGCT GCACGAGGCG
    GTGATAGAAA AGCACGTCTT CGTGGTGGAG CTGCTTCTGC TTCATGGGGC ATCAGTTCAG
    GTGGTGAACA AGCGTCAGTG CACCGCCATC GATTGTGCTG AACAGAACTC AAAAATAATG
    GAATTACTTC AAGTGGTACC GAGCTGTGTG GCGTCCGTGG ACGACGTGGG CCAAACAGAC
    CGCAAGGAGT ACGTGACGGT TAAGATCAGG AAAAAATGGA ACTCAAAAAT GTATGACCTG
    CCAGATGAAC CTTTTACCAG ACAGTTTTAC TTTGTCCCCT CAGGTGGTCA GTTTAAGGGA
    AGGACTTCAA GGGAGATTCT GGCAAGAGAT AGAAGTGTCC CTAATTTTAC TGAAGACTCT
    CTGCATGAGC CAGGGAGGCA AACAGTCACA GGCAGACAGA GCAACCTGCC AGACCAGAGC
    AGATCTGAGA CTGCTGACCG AGGAGGCGGC AATCGGCCCG AGAGGCCTGG ACCGAAACAA
    AGCGTTCCTG CCAACAGGCG GATGCTGCGC AGACACACAG TCGAGGACCC AGTCGTATCC
    GTGAGCCCAG GCTCCGCCGG CAACCCAAGC ACATCCCCAG AGGCCGATAC CGCTTCCCAG
    TCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe34769
    Clone ID Related Accession (Same CDS sequence) XM_014116765.1
    Accession Version XM_014116765.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3480bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product ankyrin repeat domain-containing protein 27
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726047.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATAGATA CCGGACCTAG AAAGGATTCA GACCCAAAAC CAGCCCCTCC ACGTGCGAGA 
    GACACTGAGA AGTGGCCTTT GGAGCTCCTG CCCTTCTGGT CGAGATTCTG GCAAGAAAGT
    GGGTGCTGGT GGCACTGCCT GCAACTCCAG GAGCAGGGTT GCTACCTCCT GCATCCGAGC
    CCACCCTTGC CACCTCTGTC TCGGGTGCAG AGATGTGGGA GCTCCGCTGG CATCTCGTCT
    GAGGAGCAGA AGGAGCATCG CTTCCCAGCT TCATTCCGAC CGCGTGGCAT CCTGCCTCTC
    ATGCGGTTTC TGTCCAAGGA GACTCTCTCT GCCCCATCTG AAGTCCATAT GGCTCTGTAT
    GATGAAGATC TCCTGAAAAA TCCTTTCTAT CTGGCTCTTC AAAAGTGGCG TCCTGACTTA
    TGCAGCAAGG TGGCCCAGAT CCATGGCATC GTCCTGGTGC CTTGCAAAGG AAGCTTGTCG
    AGCAGTGTTC AGTCCACTTG TCAGTTTGAA TCTTATATTT TGATACCCGT GGAAGAACAC
    TTTCAGACCT TGAATGGAAA GGATGTCTTT ATACAGGGAA ACAGGATTAA ATTAGGAGCT
    GGTTTCGCCT GTCTTCTCTC AGTGCCCATT CTCTTTGAAG AAACTTTCTA CAATGAAAAA
    GAAGAAAGTT TCAGCATACT GTGTATAGCC CATCCTTTGG AAAAGAGAGA GAGTTCAGAA
    GAGCCTTCAA CATCTTCCGA TCCCTTTTCC CTGAAAACCA TCGAAGACGT GAGAGAGTTT
    TTGGGAAGAC ACGCGGAGAG ATTTGACAGG AGCATTGCCT CTTTCCAGCG AACCTTCAGG
    GAATGTGAAA GAAAGAGCCT CCGTCAGCAC ATAGACTCGG TGAATGCTCT CTACACCAAA
    TGCCTCCAGC AGCTTCTGAG GGACTCTCAC CTGAAAATGC TTGCCAAGCA GGAGGCCCAG
    ATGAATCTGC TGAAGCAGGC CGTGGAGATG TATGTCCAGC ATGATATTTA TGATCTGATC
    TTTAAGTACG TGGGGACCAT GGAGGCGAGC GAGGATGCTG CCTTTAACAA AATCACAAGA
    AGCCTTCAAG ATCTTCAGCA GAAAGACATT GGTGTGAAAC CCGAGTGCAG CTTCAACATA
    CCTCGTGCAA AGAGAGAGCT GGCTCAGCTG AACAAATGCA CCTCACCACA GCAGAAGTTG
    ATTTGTTTGC GAAAGGTGGT GCAGCTCATC ACACAGTCCC CCAGCCAGAG AGTTAACTTG
    GAGACCATGT GTGCTGATGA TCTGCTGTCG GTCCTGTTGT ATTTGCTTGT GAAAACAGAT
    ATCCCTAATT GGATGGCAAA TTTGAGTTAT ATCAAGAACT TCAGATTTAG CAGCTCAGCA
    AAAGATGAAT TGGGATATTG TCTGACCTCG ATTGAGGCTG CGATTGAATA TATTCGGCAA
    GGAAGCCTCT CCGTGAAGCC ACCCGAGTCG GAGGGGTTTG GTGACAGACT GTTCCTGAAG
    CAGAGAATGA GCTTACTGTC TCAGATGACT TCAACTCCCA TTGATTGTCT GTTTAAGCAC
    ATCGCGTCAG GTAACCAGAA AGAAGTGGAA AGACTCCTGA GCCAAGATGA CCAGGATAAA
    GATGCCGTCC AAAGGATGTG TCACCCTCTG TGCTTCTGTG ACGACTGTGA GAAACTTGTC
    TCTGGGAGGC TGAACGATCC CTCCATTGTC ACTCCATTTT CCAGAGATGA CAGGGGTCAC
    ACACCGCTCC ATGTGGCCGC CCTCTGTGGG CAGGCATCCC TCATTGACTT CCTGGTTTCC
    AGAGGCGCGG GGGTAAATGC CACGGACTAC CACGGGTCGG CCCCCCTTCA CCTCGCCTGC
    CAGAAGGGCT ATCAGAGCGT CACGGTGAGC CCGGCTCCGC TGCAGCCTCG CGCGGGCCCC
    GAGGTGCAAG ACAACAACGG CAACACACCT CTCCACCTGG CCTGCGCCTA CGGGCACGAA
    GATGTAAGTG GTGCTTCCCA TCCCCATCCC CGTCCCTGTC CCCATTCCCA GGGTGCCTCA
    TCTTCCCATT TCTCAGTCGT GGAGACACAG GCCTTTCTGA CTGGCCTCCC GGCGTGCTTT
    CTTTTCATGC TTCCCTCGTT AGGGATCTCT CACGCCCCCT TCTTGTCCCA GATTCTGTCA
    ATAATGGAAG CCCATCACCT GCCCTTGGAA AGGAGGCGGA AGTCTTCAGA GGTCCCCGTG
    CAGCCCCCTC AGCGCCCCGC GGATTCCATC AGCCAGGCCT CCTCCACCTC CAGCTTCTCC
    TCCGTGTTGG CGAGCTCCAG GCAGGAGGAG CCCAAGAAGG ACTACAGAGA GGTAGAAAAA
    CTTTTAAGAG CGGTTGCTGA CGGGGATCTA GAAATGGTGC GTTACCTTTT GGAGTGGACC
    GAGGAGGACC TGGATGAATT GGAGGACGCC GCCGCTGTGG GTGATGTCGA CTTCTGTCAC
    CCCCTGTGCC AGTGTCCGAG ATGCGCTCCG GCTCAGAAGA AGCTGGCAAA GATTCCTGCC
    AGCGGGCTTG GTGTAAATGT GACCAACCAG GACGGCACCT CCCCGCTGCA TGTTGCTGCC
    CTGCACGGTC GGGCGGACCT CATCCCCCTC CTGTTGAAAC ACGGTGCCAA TGCCGGTGCC
    AGAAACGTAA ACCAAGCTGT CCCGCTCCAC CTGGCCTGCC AGAAGGGCCA CTTTCAGGTG
    GTGAGGTGTC TGTTGGATTC TAAAGCAAAA CCCAATAAAA AGGATATAAG TGGAAACACA
    CCCCTCATTT ACGCCTGTTC CAACGGCTAC CACGAAGTTG CAGCATTGCT GTTACAGCAC
    GGGGCCTCCA TCAACATCTG TAACAATAAG GGTAACACAG CGCTGCACGA GGCGGTGATA
    GAAAAGCACG TCTTCGTGGT GGAGCTGCTT CTGCTTCATG GGGCATCAGT TCAGGTGGTG
    AACAAGCGTC AGTGCACCGC CATCGATTGT GCTGAACAGA ACTCAAAAAT AATGGAATTA
    CTTCAAGTGG TACCGAGCTG TGTGGCGTCC GTGGACGACG TGGGCCAAAC AGACCGCAAG
    GAGTACGTGA CGGTTAAGAT CAGGAAAAAA TGGAACTCAA AAATGTATGA CCTGCCAGAT
    GAACCTTTTA CCAGACAGTT TTACTTTGTC CCCTCAGGTG GTCAGTTTAA GGGAAGGACT
    TCAAGGGAGA TTCTGGCAAG AGATAGAAGT GTCCCTAATT TTACTGAAGA CTCTCTGCAT
    GAGCCAGGGA GGCAAACAGT CACAGGCAGA CAGAGCAACC TGCCAGACCA GAGCAGATCT
    GAGACTGCTG ACCGAGGAGG CGGCAATCGG CCCGAGAGGC CTGGACCGAA ACAAAGCGTT
    CCTGCCAACA GGCGGATGCT GCGCAGACAC ACAGTCGAGG ACCCAGTCGT ATCCGTGAGC
    CCAGGCTCCG CCGGCAACCC AAGCACATCC CCAGAGGCCG ATACCGCTTC CCAGTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013972240.1
    CDS172..3651
    Translation

    Target ORF information:

    RefSeq Version XM_014116765.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris ankyrin repeat domain 27 (VPS9 domain) (ANKRD27), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014116765.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGATAGATA CCGGACCTAG AAAGGATTCA GACCCAAAAC CAGCCCCTCC ACGTGCGAGA 
    GACACTGAGA AGTGGCCTTT GGAGCTCCTG CCCTTCTGGT CGAGATTCTG GCAAGAAAGT
    GGGTGCTGGT GGCACTGCCT GCAACTCCAG GAGCAGGGTT GCTACCTCCT GCATCCGAGC
    CCACCCTTGC CACCTCTGTC TCGGGTGCAG AGATGTGGGA GCTCCGCTGG CATCTCGTCT
    GAGGAGCAGA AGGAGCATCG CTTCCCAGCT TCATTCCGAC CGCGTGGCAT CCTGCCTCTC
    ATGCGGTTTC TGTCCAAGGA GACTCTCTCT GCCCCATCTG AAGTCCATAT GGCTCTGTAT
    GATGAAGATC TCCTGAAAAA TCCTTTCTAT CTGGCTCTTC AAAAGTGGCG TCCTGACTTA
    TGCAGCAAGG TGGCCCAGAT CCATGGCATC GTCCTGGTGC CTTGCAAAGG AAGCTTGTCG
    AGCAGTGTTC AGTCCACTTG TCAGTTTGAA TCTTATATTT TGATACCCGT GGAAGAACAC
    TTTCAGACCT TGAATGGAAA GGATGTCTTT ATACAGGGAA ACAGGATTAA ATTAGGAGCT
    GGTTTCGCCT GTCTTCTCTC AGTGCCCATT CTCTTTGAAG AAACTTTCTA CAATGAAAAA
    GAAGAAAGTT TCAGCATACT GTGTATAGCC CATCCTTTGG AAAAGAGAGA GAGTTCAGAA
    GAGCCTTCAA CATCTTCCGA TCCCTTTTCC CTGAAAACCA TCGAAGACGT GAGAGAGTTT
    TTGGGAAGAC ACGCGGAGAG ATTTGACAGG AGCATTGCCT CTTTCCAGCG AACCTTCAGG
    GAATGTGAAA GAAAGAGCCT CCGTCAGCAC ATAGACTCGG TGAATGCTCT CTACACCAAA
    TGCCTCCAGC AGCTTCTGAG GGACTCTCAC CTGAAAATGC TTGCCAAGCA GGAGGCCCAG
    ATGAATCTGC TGAAGCAGGC CGTGGAGATG TATGTCCAGC ATGATATTTA TGATCTGATC
    TTTAAGTACG TGGGGACCAT GGAGGCGAGC GAGGATGCTG CCTTTAACAA AATCACAAGA
    AGCCTTCAAG ATCTTCAGCA GAAAGACATT GGTGTGAAAC CCGAGTGCAG CTTCAACATA
    CCTCGTGCAA AGAGAGAGCT GGCTCAGCTG AACAAATGCA CCTCACCACA GCAGAAGTTG
    ATTTGTTTGC GAAAGGTGGT GCAGCTCATC ACACAGTCCC CCAGCCAGAG AGTTAACTTG
    GAGACCATGT GTGCTGATGA TCTGCTGTCG GTCCTGTTGT ATTTGCTTGT GAAAACAGAT
    ATCCCTAATT GGATGGCAAA TTTGAGTTAT ATCAAGAACT TCAGATTTAG CAGCTCAGCA
    AAAGATGAAT TGGGATATTG TCTGACCTCG ATTGAGGCTG CGATTGAATA TATTCGGCAA
    GGAAGCCTCT CCGTGAAGCC ACCCGAGTCG GAGGGGTTTG GTGACAGACT GTTCCTGAAG
    CAGAGAATGA GCTTACTGTC TCAGATGACT TCAACTCCCA TTGATTGTCT GTTTAAGCAC
    ATCGCGTCAG GTAACCAGAA AGAAGTGGAA AGACTCCTGA GCCAAGATGA CCAGGATAAA
    GATGCCGTCC AAAGGATGTG TCACCCTCTG TGCTTCTGTG ACGACTGTGA GAAACTTGTC
    TCTGGGAGGC TGAACGATCC CTCCATTGTC ACTCCATTTT CCAGAGATGA CAGGGGTCAC
    ACACCGCTCC ATGTGGCCGC CCTCTGTGGG CAGGCATCCC TCATTGACTT CCTGGTTTCC
    AGAGGCGCGG GGGTAAATGC CACGGACTAC CACGGGTCGG CCCCCCTTCA CCTCGCCTGC
    CAGAAGGGCT ATCAGAGCGT CACGGTGAGC CCGGCTCCGC TGCAGCCTCG CGCGGGCCCC
    GAGGTGCAAG ACAACAACGG CAACACACCT CTCCACCTGG CCTGCGCCTA CGGGCACGAA
    GATGTAAGTG GTGCTTCCCA TCCCCATCCC CGTCCCTGTC CCCATTCCCA GGGTGCCTCA
    TCTTCCCATT TCTCAGTCGT GGAGACACAG GCCTTTCTGA CTGGCCTCCC GGCGTGCTTT
    CTTTTCATGC TTCCCTCGTT AGGGATCTCT CACGCCCCCT TCTTGTCCCA GATTCTGTCA
    ATAATGGAAG CCCATCACCT GCCCTTGGAA AGGAGGCGGA AGTCTTCAGA GGTCCCCGTG
    CAGCCCCCTC AGCGCCCCGC GGATTCCATC AGCCAGGCCT CCTCCACCTC CAGCTTCTCC
    TCCGTGTTGG CGAGCTCCAG GCAGGAGGAG CCCAAGAAGG ACTACAGAGA GGTAGAAAAA
    CTTTTAAGAG CGGTTGCTGA CGGGGATCTA GAAATGGTGC GTTACCTTTT GGAGTGGACC
    GAGGAGGACC TGGATGAATT GGAGGACGCC GCCGCTGTGG GTGATGTCGA CTTCTGTCAC
    CCCCTGTGCC AGTGTCCGAG ATGCGCTCCG GCTCAGAAGA AGCTGGCAAA GATTCCTGCC
    AGCGGGCTTG GTGTAAATGT GACCAACCAG GACGGCACCT CCCCGCTGCA TGTTGCTGCC
    CTGCACGGTC GGGCGGACCT CATCCCCCTC CTGTTGAAAC ACGGTGCCAA TGCCGGTGCC
    AGAAACGTAA ACCAAGCTGT CCCGCTCCAC CTGGCCTGCC AGAAGGGCCA CTTTCAGGTG
    GTGAGGTGTC TGTTGGATTC TAAAGCAAAA CCCAATAAAA AGGATATAAG TGGAAACACA
    CCCCTCATTT ACGCCTGTTC CAACGGCTAC CACGAAGTTG CAGCATTGCT GTTACAGCAC
    GGGGCCTCCA TCAACATCTG TAACAATAAG GGTAACACAG CGCTGCACGA GGCGGTGATA
    GAAAAGCACG TCTTCGTGGT GGAGCTGCTT CTGCTTCATG GGGCATCAGT TCAGGTGGTG
    AACAAGCGTC AGTGCACCGC CATCGATTGT GCTGAACAGA ACTCAAAAAT AATGGAATTA
    CTTCAAGTGG TACCGAGCTG TGTGGCGTCC GTGGACGACG TGGGCCAAAC AGACCGCAAG
    GAGTACGTGA CGGTTAAGAT CAGGAAAAAA TGGAACTCAA AAATGTATGA CCTGCCAGAT
    GAACCTTTTA CCAGACAGTT TTACTTTGTC CCCTCAGGTG GTCAGTTTAA GGGAAGGACT
    TCAAGGGAGA TTCTGGCAAG AGATAGAAGT GTCCCTAATT TTACTGAAGA CTCTCTGCAT
    GAGCCAGGGA GGCAAACAGT CACAGGCAGA CAGAGCAACC TGCCAGACCA GAGCAGATCT
    GAGACTGCTG ACCGAGGAGG CGGCAATCGG CCCGAGAGGC CTGGACCGAA ACAAAGCGTT
    CCTGCCAACA GGCGGATGCT GCGCAGACAC ACAGTCGAGG ACCCAGTCGT ATCCGTGAGC
    CCAGGCTCCG CCGGCAACCC AAGCACATCC CCAGAGGCCG ATACCGCTTC CCAGTCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.