ANKRD27 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKRD27
Entrez Gene ID	84079
Full Name	ankyrin repeat domain 27
Synonyms	PP12899,VARP
General protein information	Preferred Names ankyrin repeat domain 27 Names ankyrin repeat domain-containing protein 27 VPS9 domain-containing protein VPS9-ankyrin-repeat protein Vps9 domain and ankyrin-repeat-containing protein ankyrin repeat domain 27 (VPS9 domain)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.11

mRNA and Protein(s)

mRNA	Protein	Name
XM_017027349.1	XP_016882838.1	ankyrin repeat domain-containing protein 27 isoform X2
XM_006723412.2	XP_006723475.1	ankyrin repeat domain-containing protein 27 isoform X1
NM_032139.2	NP_115515.2	ankyrin repeat domain-containing protein 27
NM_032139.3	NP_115515.2	ankyrin repeat domain-containing protein 27

Pathways from BioSystems

Homologies

	LOC716232	XP_001106852.1
Rattus norvegicus (Norway rat)	Ankrd27	NP_001258193.1
Pan troglodytes (chimpanzee)	ANKRD27	XP_001151730.1
Bos taurus (cattle)	ANKRD27	NP_001096002.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003151	XP_001688409.2
Homo sapiens (human)	ANKRD27	NP_115515.2
Danio rerio (zebrafish)	wu:fc41e04	XP_005163257.1
Xenopus tropicalis (tropical clawed frog)	ankrd27	XP_004913610.1
Canis lupus familiaris (dog)	ANKRD27	XP_005616956.1
Mus musculus (house mouse)	Ankrd27	NP_663608.3
Gallus gallus (chicken)	ANKRD27	NP_001129150.1

Gene Ontology
Bibliography

Related articles in PubMed

Multiple Roles of VARP in Endosomal Trafficking: Rabs, Retromer Components and R-SNARE VAMP7 Meet on VARP.
Fukuda M
Traffic (Copenhagen, Denmark)17(7)709-19(2016 07)

GWAS identifies four novel eosinophilic esophagitis loci.
Sleiman PM, Wang ML, Cianferoni A, Aceves S, Gonsalves N, Nadeau K, Bredenoord AJ, Furuta GT, Spergel JM, Hakonarson H
Nature communications55593(2014 Nov)

VARP is recruited on to endosomes by direct interaction with retromer, where together they function in export to the cell surface.
Hesketh GG, P?rez-Dorado I, Jackson LP, Wartosch L, Sch?fer IB, Gray SR, McCoy AJ, Zeldin OB, Garman EF, Harbour ME, Evans PR, Seaman MNJ, Luzio JP, Owen DJ
Developmental cell29(5)591-606(2014 Jun)

Common genetic variation and antidepressant efficacy in major depressive disorder: a meta-analysis of three genome-wide pharmacogenetic studies.
, ,
The American journal of psychiatry170(2)207-17(2013 Feb)

The binding of Varp to VAMP7 traps VAMP7 in a closed, fusogenically inactive conformation.
Sch?fer IB, Hesketh GG, Bright NA, Gray SR, Pryor PR, Evans PR, Luzio JP, Owen DJ
Nature structural & molecular biology19(12)1300-9(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

VARP regulates endosomal trafficking and and retromer-mediated endosome-to-plasma membrane sorting of transmembrane proteins by binding to rab GTP-Binding Proteins and R-SNARE VAMP7 [review].
Title: Multiple Roles of VARP in Endosomal Trafficking: Rabs, Retromer Components and R-SNARE VAMP7 Meet on VARP.

Eosinophilic esophagitis was associate with increased expression of ANKRD27.
Title: GWAS identifies four novel eosinophilic esophagitis loci.

VAMP7-SNARE motif is trapped between Varp and the VAMP7 longin domain, and hence Varp kinetically inhibits the ability of VAMP7 to form SNARE complexes
Title: The binding of Varp to VAMP7 traps VAMP7 in a closed, fusogenically inactive conformation.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

The following ANKRD27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKRD27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu04857	NM_032139.3 Latest version!	Homo sapiens ankyrin repeat domain 27 (ANKRD27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu04857	XM_006723412.2 Latest version!	Homo sapiens ankyrin repeat domain 27 (ANKRD27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu52519	XM_017027349.1 Latest version!	Homo sapiens ankyrin repeat domain 27 (ANKRD27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
View Old Accession Versions >>
OHu04857	NM_032139.2	Homo sapiens ankyrin repeat domain 27 (ANKRD27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKRD27 ( NM_032139.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_115515.2 Homo sapiens ankyrin repeat domain 27 (ANKRD27), mRNA.

Price & Availability↑

CloneID	OHu04857
Clone ID Related Accession (Same CDS sequence)	XM_006723412.2 , NM_032139.2 , NM_032139.3
Accession Version	NM_032139.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 27
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI462844.1, AK054561.1 and AA903176.1. On Oct 12, 2006 this sequence version replaced NM_032139.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC045605.1, AK054561.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCTCTGT ATGATGAAGA CCTCCTGAAA AATCCTTTCT ATCTGGCTCT GCAAAAGTGC 
CGCCCTGACT TGTGCAGCAA AGTGGCCCAA ATCCATGGCA TTGTCTTAGT ACCCTGCAAA 
GGAAGCCTGT CGAGCAGCAT CCAGTCTACT TGTCAGTTTG AGTCCTACAT TTTGATACCT 
GTGGAAGAGC ATTTTCAGAC CTTAAATGGA AAGGATGTCT TTATTCAAGG GAACAGGATT 
AAATTAGGAG CTGGTTTTGC CTGTCTTCTC TCAGTGCCCA TTCTCTTTGA AGAAACTTTC 
TACAATGAAA AAGAAGAGAG TTTCAGCATC CTGTGTATAG CCCATCCTTT GGAAAAGAGA 
GAGAGTTCAG AAGAGCCTTT GGCACCCTCA GATCCCTTTT CCCTGAAAAC CATTGAAGAT 
GTGAGAGAGT TCTTGGGAAG ACACTCCGAG CGATTTGACA GGAACATCGC CTCTTTCCAT 
CGAACATTCC GAGAATGCGA GAGAAAGAGC CTCCGTCACC ACATAGACTC AGCGAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTG AGGGACTCTC ACCTGAAAAT GCTCGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTGGAGA TATACGTCCA TCATGAAATT 
TACAACCTGA TCTTTAAATA CGTGGGGACC ATGGAGGCAA GTGAGGATGC GGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTTCAG CAGAAAGATA TTGGTGTGAA ACCGGAGTTC 
AGCTTTAACA TACCTCGTGC CAAAAGAGAG CTGGCTCAGC TGAACAAATG CACCTCCCCA 
CAGCAGAAGC TTGTCTGCTT GCGAAAAGTG GTGCAGCTCA TTACACAGTC TCCAAGCCAG 
AGAGTGAACC TGGAGACCAT GTGTGCTGAT GATCTGCTAT CAGTCCTGTT ATACTTGCTT 
GTGAAAACGG AGATCCCTAA TTGGATGGCA AATTTGAGTT ACATCAAAAA CTTCAGGTTT 
AGCAGCTTGG CAAAGGATGA ACTGGGATAC TGCCTGACCT CATTCGAAGC TGCCATTGAA 
TATATTCGGC AAGGAAGCCT CTCTGCTAAA CCCCCTGAGT CTGAGGGATT TGGAGACAGG 
CTGTTCCTTA AGCAGAGAAT GAGCTTACTC TCTCAGATGA CTTCGTCTCC CACCGACTGC 
CTGTTTAAGC ACATTGCATC AGGTAACCAG AAAGAAGTGG AGAGACTTCT GAGCCAAGAG 
GACCATGATA AAGATACCGT CCAAAAGATG TGTCACCCTC TCTGCTTCTG CGATGACTGT 
GAGAAACTCG TCTCTGGGAG GTTGAATGAT CCCTCAGTTG TCACTCCATT CTCCAGAGAC 
GACAGGGGGC ACACCCCTCT CCATGTGGCT GCTGTCTGTG GGCAGGCATC CCTCATCGAC 
CTCCTGGTTT CCAAGGGCGC CATGGTAAAT GCCACAGACT ACCATGGAGC CACTCCGCTC 
CACCTGGCCT GTCAGAAGGG CTACCAGAGC GTGACGCTGC TGCTGCTGCA CTACAAGGCC 
AGCGCGGAAG TGCAGGACAA CAATGGGAAT ACGCCACTCC ACCTGGCCTG CACCTACGGC 
CACGAGGACT GTGTGAAGGC TCTGGTTTAC TACGACGTGG AGTCGTGCAG ACTTGACATT 
GGCAATGAGA AAGGAGACAC CCCTCTACAC ATTGCTGCCC GCTGGGGCTA CCAAGGCGTC 
ATAGAGACAT TGCTGCAGAA CGGAGCGTCC ACCGAGATCC AGAACAGACT GAAGGAGACG 
CCCCTCAAGT GTGCATTAAA CTCAAAGATT CTGTCTGTAA TGGAAGCCTA TCACCTGTCC 
TTCGAGAGGA GGCAGAAGTC GTCCGAGGCC CCTGTGCAGT CCCCGCAGCG CTCCGTGGAC 
TCCATCAGCC AAGAGTCCTC CACTTCCAGC TTCTCCTCCA TGTCAGCCAG CTCAAGGCAG 
GAGGAGACCA AGAAGGACTA CAGAGAGGTA GAAAAACTTT TGAGAGCAGT TGCTGATGGA 
GATCTAGAAA TGGTGCGTTA CCTGTTGGAA TGGACAGAGG AGGACCTGGA GGATGCGGAG 
GACACTGTCA GTGCAGCGGA CCCCGAATTC TGTCACCCGT TGTGCCAGTG CCCCAAGTGT 
GCCCCAGCTC AGAAGAGGCT GGCGAAGGTT CCTGCCAGTG GGCTTGGTGT GAACGTGACC 
AGCCAGGACG GCTCCTCCCC GCTGCATGTC GCCGCCCTGC ACGGCCGGGC GGACCTCATC 
CCCCTCCTGC TGAAGCACGG GGCCAACGCA GGTGCCAGGA ACGCAGACCA AGCCGTCCCG 
CTCCACCTGG CCTGCCAGCA GGGCCACTTT CAGGTGGTGA AGTGTCTGTT AGATTCGAAT 
GCAAAACCCA ATAAGAAGGA CCTCAGTGGA AACACGCCCC TCATTTACGC CTGCTCCGGT 
GGCCATCACG AGCTTGTGGC ACTGCTGCTA CAGCACGGGG CCTCCATTAA CGCTTCTAAC 
AATAAGGGCA ACACAGCGCT GCACGAGGCT GTGATTGAAA AGCACGTCTT CGTGGTAGAG 
CTGCTTCTGC TCCACGGAGC GTCAGTTCAG GTGCTGAACA AGCGGCAGCG CACGGCTGTA 
GACTGTGCTG AACAGAATTC AAAAATAATG GAATTGCTTC AGGTGGTACC AAGCTGTGTT 
GCTTCATTAG ATGATGTGGC TGAAACTGAC CGCAAGGAGT ATGTCACTGT TAAGATCAGG 
AAAAAATGGA ACTCAAAACT GTATGATCTA CCAGATGAGC CTTTTACAAG ACAGTTTTAC 
TTTGTCCACT CAGCTGGTCA GTTTAAGGGA AAGACTTCAA GGGAGATTAT GGCAAGAGAT 
AGAAGTGTCC CTAATTTAAC CGAAGGTTCT TTGCATGAGC CAGGGAGGCA AAGTGTCACA 
CTGAGACAGA ATAACCTGCC AGCTCAGAGT GGATCTCATG CTGCTGAGAA AGGCAACAGC 
GACTGGCCAG AGAGGCCTGG ACTGACACAG ACTGGCCCTG GACACAGACG GATGCTGCGG 
AGACACACGG TAGAGGATGC GGTCGTGTCC CAGGGCCCGG AGGCTGCTGG CCCCCTCTCC 
ACTCCCCAAG AGGTTAGTGC TTCCCGGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115515.2
CDS	157..3309
Translation	MALYDEDLLKNPFYLALQKCRPDLCSKVAQIHGIVLVPCKGSLSSSIQSTCQFESYILIPVEEHFQTLNGKDVFIQGNRIKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLEKRESSEEPLAPSDPFSLKTIEDVREFLGRHSERFDRNIASFHRTFRECERKSLRHHIDSANALYTKCLQQLLRDSHLKMLAKQEAQMNLMKQAVEIYVHHEIYNLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELAQLNKCTSPQQKLVCLRKVVQLITQSPSQRVNLETMCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSLAKDELGYCLTSFEAAIEYIRQGSLSAKPPESEGFGDRLFLKQRMSLLSQMTSSPTDCLFKHIASGNQKEVERLLSQEDHDKDTVQKMCHPLCFCDDCEKLVSGRLNDPSVVTPFSRDDRGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKASAEVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKETPLKCALNSKILSVMEAYHLSFERRQKSSEAPVQSPQRSVDSISQESSTSSFSSMSASSRQEETKKDYREVEKLLRAVADGDLEMVRYLLEWTEEDLEDAEDTVSAADPEFCHPLCQCPKCAPAQKRLAKVPASGLGVNVTSQDGSSPLHVAALHGRADLIPLLLKHGANAGARNADQAVPLHLACQQGHFQVVKCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEKHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNSKIMELLQVVPSCVASLDDVAETDRKEYVTVKIRKKWNSKLYDLPDEPFTRQFYFVHSAGQFKGKTSREIMARDRSVPNLTEGSLHEPGRQSVTLRQNNLPAQSGSHAAEKGNSDWPERPGLTQTGPGHRRMLRRHTVEDAVVSQGPEAAGPLSTPQEVSASRS

Target ORF information:

RefSeq Version	NM_032139.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 27 (ANKRD27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032139.2

ORF Insert Sequence:

ATGGCTCTGT ATGATGAAGA CCTCCTGAAA AATCCTTTCT ATCTGGCTCT GCAAAAGTGC 
CGCCCTGACT TGTGCAGCAA AGTGGCCCAA ATCCATGGCA TTGTCTTAGT ACCCTGCAAA 
GGAAGCCTGT CGAGCAGCAT CCAGTCTACT TGTCAGTTTG AGTCCTACAT TTTGATACCT 
GTGGAAGAGC ATTTTCAGAC CTTAAATGGA AAGGATGTCT TTATTCAAGG GAACAGGATT 
AAATTAGGAG CTGGTTTTGC CTGTCTTCTC TCAGTGCCCA TTCTCTTTGA AGAAACTTTC 
TACAATGAAA AAGAAGAGAG TTTCAGCATC CTGTGTATAG CCCATCCTTT GGAAAAGAGA 
GAGAGTTCAG AAGAGCCTTT GGCACCCTCA GATCCCTTTT CCCTGAAAAC CATTGAAGAT 
GTGAGAGAGT TCTTGGGAAG ACACTCCGAG CGATTTGACA GGAACATCGC CTCTTTCCAT 
CGAACATTCC GAGAATGCGA GAGAAAGAGC CTCCGTCACC ACATAGACTC AGCGAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTG AGGGACTCTC ACCTGAAAAT GCTCGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTGGAGA TATACGTCCA TCATGAAATT 
TACAACCTGA TCTTTAAATA CGTGGGGACC ATGGAGGCAA GTGAGGATGC GGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTTCAG CAGAAAGATA TTGGTGTGAA ACCGGAGTTC 
AGCTTTAACA TACCTCGTGC CAAAAGAGAG CTGGCTCAGC TGAACAAATG CACCTCCCCA 
CAGCAGAAGC TTGTCTGCTT GCGAAAAGTG GTGCAGCTCA TTACACAGTC TCCAAGCCAG 
AGAGTGAACC TGGAGACCAT GTGTGCTGAT GATCTGCTAT CAGTCCTGTT ATACTTGCTT 
GTGAAAACGG AGATCCCTAA TTGGATGGCA AATTTGAGTT ACATCAAAAA CTTCAGGTTT 
AGCAGCTTGG CAAAGGATGA ACTGGGATAC TGCCTGACCT CATTCGAAGC TGCCATTGAA 
TATATTCGGC AAGGAAGCCT CTCTGCTAAA CCCCCTGAGT CTGAGGGATT TGGAGACAGG 
CTGTTCCTTA AGCAGAGAAT GAGCTTACTC TCTCAGATGA CTTCGTCTCC CACCGACTGC 
CTGTTTAAGC ACATTGCATC AGGTAACCAG AAAGAAGTGG AGAGACTTCT GAGCCAAGAG 
GACCATGATA AAGATACCGT CCAAAAGATG TGTCACCCTC TCTGCTTCTG CGATGACTGT 
GAGAAACTCG TCTCTGGGAG GTTGAATGAT CCCTCAGTTG TCACTCCATT CTCCAGAGAC 
GACAGGGGGC ACACCCCTCT CCATGTGGCT GCTGTCTGTG GGCAGGCATC CCTCATCGAC 
CTCCTGGTTT CCAAGGGCGC CATGGTAAAT GCCACAGACT ACCATGGAGC CACTCCGCTC 
CACCTGGCCT GTCAGAAGGG CTACCAGAGC GTGACGCTGC TGCTGCTGCA CTACAAGGCC 
AGCGCGGAAG TGCAGGACAA CAATGGGAAT ACGCCACTCC ACCTGGCCTG CACCTACGGC 
CACGAGGACT GTGTGAAGGC TCTGGTTTAC TACGACGTGG AGTCGTGCAG ACTTGACATT 
GGCAATGAGA AAGGAGACAC CCCTCTACAC ATTGCTGCCC GCTGGGGCTA CCAAGGCGTC 
ATAGAGACAT TGCTGCAGAA CGGAGCGTCC ACCGAGATCC AGAACAGACT GAAGGAGACG 
CCCCTCAAGT GTGCATTAAA CTCAAAGATT CTGTCTGTAA TGGAAGCCTA TCACCTGTCC 
TTCGAGAGGA GGCAGAAGTC GTCCGAGGCC CCTGTGCAGT CCCCGCAGCG CTCCGTGGAC 
TCCATCAGCC AAGAGTCCTC CACTTCCAGC TTCTCCTCCA TGTCAGCCAG CTCAAGGCAG 
GAGGAGACCA AGAAGGACTA CAGAGAGGTA GAAAAACTTT TGAGAGCAGT TGCTGATGGA 
GATCTAGAAA TGGTGCGTTA CCTGTTGGAA TGGACAGAGG AGGACCTGGA GGATGCGGAG 
GACACTGTCA GTGCAGCGGA CCCCGAATTC TGTCACCCGT TGTGCCAGTG CCCCAAGTGT 
GCCCCAGCTC AGAAGAGGCT GGCGAAGGTT CCTGCCAGTG GGCTTGGTGT GAACGTGACC 
AGCCAGGACG GCTCCTCCCC GCTGCATGTC GCCGCCCTGC ACGGCCGGGC GGACCTCATC 
CCCCTCCTGC TGAAGCACGG GGCCAACGCA GGTGCCAGGA ACGCAGACCA AGCCGTCCCG 
CTCCACCTGG CCTGCCAGCA GGGCCACTTT CAGGTGGTGA AGTGTCTGTT AGATTCGAAT 
GCAAAACCCA ATAAGAAGGA CCTCAGTGGA AACACGCCCC TCATTTACGC CTGCTCCGGT 
GGCCATCACG AGCTTGTGGC ACTGCTGCTA CAGCACGGGG CCTCCATTAA CGCTTCTAAC 
AATAAGGGCA ACACAGCGCT GCACGAGGCT GTGATTGAAA AGCACGTCTT CGTGGTAGAG 
CTGCTTCTGC TCCACGGAGC GTCAGTTCAG GTGCTGAACA AGCGGCAGCG CACGGCTGTA 
GACTGTGCTG AACAGAATTC AAAAATAATG GAATTGCTTC AGGTGGTACC AAGCTGTGTT 
GCTTCATTAG ATGATGTGGC TGAAACTGAC CGCAAGGAGT ATGTCACTGT TAAGATCAGG 
AAAAAATGGA ACTCAAAACT GTATGATCTA CCAGATGAGC CTTTTACAAG ACAGTTTTAC 
TTTGTCCACT CAGCTGGTCA GTTTAAGGGA AAGACTTCAA GGGAGATTAT GGCAAGAGAT 
AGAAGTGTCC CTAATTTAAC CGAAGGTTCT TTGCATGAGC CAGGGAGGCA AAGTGTCACA 
CTGAGACAGA ATAACCTGCC AGCTCAGAGT GGATCTCATG CTGCTGAGAA AGGCAACAGC 
GACTGGCCAG AGAGGCCTGG ACTGACACAG ACTGGCCCTG GACACAGACG GATGCTGCGG 
AGACACACGG TAGAGGATGC GGTCGTGTCC CAGGGCCCGG AGGCTGCTGG CCCCCTCTCC 
ACTCCCCAAG AGGTTAGTGC TTCCCGGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD27 ( NM_032139.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_115515.2 Homo sapiens ankyrin repeat domain 27 (ANKRD27), mRNA.

Price & Availability↑

CloneID	OHu04857
Clone ID Related Accession (Same CDS sequence)	XM_006723412.2 , NM_032139.2 , NM_032139.3
Accession Version	NM_032139.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 27
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK054561.1 and AA903176.1. On Nov 23, 2018 this sequence version replaced NM_032139.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.127942.1, BC045605.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000306065.9/ ENSP00000304292.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTCTGT ATGATGAAGA CCTCCTGAAA AATCCTTTCT ATCTGGCTCT GCAAAAGTGC 
CGCCCTGACT TGTGCAGCAA AGTGGCCCAA ATCCATGGCA TTGTCTTAGT ACCCTGCAAA 
GGAAGCCTGT CGAGCAGCAT CCAGTCTACT TGTCAGTTTG AGTCCTACAT TTTGATACCT 
GTGGAAGAGC ATTTTCAGAC CTTAAATGGA AAGGATGTCT TTATTCAAGG GAACAGGATT 
AAATTAGGAG CTGGTTTTGC CTGTCTTCTC TCAGTGCCCA TTCTCTTTGA AGAAACTTTC 
TACAATGAAA AAGAAGAGAG TTTCAGCATC CTGTGTATAG CCCATCCTTT GGAAAAGAGA 
GAGAGTTCAG AAGAGCCTTT GGCACCCTCA GATCCCTTTT CCCTGAAAAC CATTGAAGAT 
GTGAGAGAGT TCTTGGGAAG ACACTCCGAG CGATTTGACA GGAACATCGC CTCTTTCCAT 
CGAACATTCC GAGAATGCGA GAGAAAGAGC CTCCGTCACC ACATAGACTC AGCGAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTG AGGGACTCTC ACCTGAAAAT GCTCGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTGGAGA TATACGTCCA TCATGAAATT 
TACAACCTGA TCTTTAAATA CGTGGGGACC ATGGAGGCAA GTGAGGATGC GGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTTCAG CAGAAAGATA TTGGTGTGAA ACCGGAGTTC 
AGCTTTAACA TACCTCGTGC CAAAAGAGAG CTGGCTCAGC TGAACAAATG CACCTCCCCA 
CAGCAGAAGC TTGTCTGCTT GCGAAAAGTG GTGCAGCTCA TTACACAGTC TCCAAGCCAG 
AGAGTGAACC TGGAGACCAT GTGTGCTGAT GATCTGCTAT CAGTCCTGTT ATACTTGCTT 
GTGAAAACGG AGATCCCTAA TTGGATGGCA AATTTGAGTT ACATCAAAAA CTTCAGGTTT 
AGCAGCTTGG CAAAGGATGA ACTGGGATAC TGCCTGACCT CATTCGAAGC TGCCATTGAA 
TATATTCGGC AAGGAAGCCT CTCTGCTAAA CCCCCTGAGT CTGAGGGATT TGGAGACAGG 
CTGTTCCTTA AGCAGAGAAT GAGCTTACTC TCTCAGATGA CTTCGTCTCC CACCGACTGC 
CTGTTTAAGC ACATTGCATC AGGTAACCAG AAAGAAGTGG AGAGACTTCT GAGCCAAGAG 
GACCATGATA AAGATACCGT CCAAAAGATG TGTCACCCTC TCTGCTTCTG CGATGACTGT 
GAGAAACTCG TCTCTGGGAG GTTGAATGAT CCCTCAGTTG TCACTCCATT CTCCAGAGAC 
GACAGGGGGC ACACCCCTCT CCATGTGGCT GCTGTCTGTG GGCAGGCATC CCTCATCGAC 
CTCCTGGTTT CCAAGGGCGC CATGGTAAAT GCCACAGACT ACCATGGAGC CACTCCGCTC 
CACCTGGCCT GTCAGAAGGG CTACCAGAGC GTGACGCTGC TGCTGCTGCA CTACAAGGCC 
AGCGCGGAAG TGCAGGACAA CAATGGGAAT ACGCCACTCC ACCTGGCCTG CACCTACGGC 
CACGAGGACT GTGTGAAGGC TCTGGTTTAC TACGACGTGG AGTCGTGCAG ACTTGACATT 
GGCAATGAGA AAGGAGACAC CCCTCTACAC ATTGCTGCCC GCTGGGGCTA CCAAGGCGTC 
ATAGAGACAT TGCTGCAGAA CGGAGCGTCC ACCGAGATCC AGAACAGACT GAAGGAGACG 
CCCCTCAAGT GTGCATTAAA CTCAAAGATT CTGTCTGTAA TGGAAGCCTA TCACCTGTCC 
TTCGAGAGGA GGCAGAAGTC GTCCGAGGCC CCTGTGCAGT CCCCGCAGCG CTCCGTGGAC 
TCCATCAGCC AAGAGTCCTC CACTTCCAGC TTCTCCTCCA TGTCAGCCAG CTCAAGGCAG 
GAGGAGACCA AGAAGGACTA CAGAGAGGTA GAAAAACTTT TGAGAGCAGT TGCTGATGGA 
GATCTAGAAA TGGTGCGTTA CCTGTTGGAA TGGACAGAGG AGGACCTGGA GGATGCGGAG 
GACACTGTCA GTGCAGCGGA CCCCGAATTC TGTCACCCGT TGTGCCAGTG CCCCAAGTGT 
GCCCCAGCTC AGAAGAGGCT GGCGAAGGTT CCTGCCAGTG GGCTTGGTGT GAACGTGACC 
AGCCAGGACG GCTCCTCCCC GCTGCATGTC GCCGCCCTGC ACGGCCGGGC GGACCTCATC 
CCCCTCCTGC TGAAGCACGG GGCCAACGCA GGTGCCAGGA ACGCAGACCA AGCCGTCCCG 
CTCCACCTGG CCTGCCAGCA GGGCCACTTT CAGGTGGTGA AGTGTCTGTT AGATTCGAAT 
GCAAAACCCA ATAAGAAGGA CCTCAGTGGA AACACGCCCC TCATTTACGC CTGCTCCGGT 
GGCCATCACG AGCTTGTGGC ACTGCTGCTA CAGCACGGGG CCTCCATTAA CGCTTCTAAC 
AATAAGGGCA ACACAGCGCT GCACGAGGCT GTGATTGAAA AGCACGTCTT CGTGGTAGAG 
CTGCTTCTGC TCCACGGAGC GTCAGTTCAG GTGCTGAACA AGCGGCAGCG CACGGCTGTA 
GACTGTGCTG AACAGAATTC AAAAATAATG GAATTGCTTC AGGTGGTACC AAGCTGTGTT 
GCTTCATTAG ATGATGTGGC TGAAACTGAC CGCAAGGAGT ATGTCACTGT TAAGATCAGG 
AAAAAATGGA ACTCAAAACT GTATGATCTA CCAGATGAGC CTTTTACAAG ACAGTTTTAC 
TTTGTCCACT CAGCTGGTCA GTTTAAGGGA AAGACTTCAA GGGAGATTAT GGCAAGAGAT 
AGAAGTGTCC CTAATTTAAC CGAAGGTTCT TTGCATGAGC CAGGGAGGCA AAGTGTCACA 
CTGAGACAGA ATAACCTGCC AGCTCAGAGT GGATCTCATG CTGCTGAGAA AGGCAACAGC 
GACTGGCCAG AGAGGCCTGG ACTGACACAG ACTGGCCCTG GACACAGACG GATGCTGCGG 
AGACACACGG TAGAGGATGC GGTCGTGTCC CAGGGCCCGG AGGCTGCTGG CCCCCTCTCC 
ACTCCCCAAG AGGTTAGTGC TTCCCGGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_115515.2
CDS	141..3293
Translation	MALYDEDLLKNPFYLALQKCRPDLCSKVAQIHGIVLVPCKGSLSSSIQSTCQFESYILIPVEEHFQTLNGKDVFIQGNRIKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLEKRESSEEPLAPSDPFSLKTIEDVREFLGRHSERFDRNIASFHRTFRECERKSLRHHIDSANALYTKCLQQLLRDSHLKMLAKQEAQMNLMKQAVEIYVHHEIYNLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELAQLNKCTSPQQKLVCLRKVVQLITQSPSQRVNLETMCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSLAKDELGYCLTSFEAAIEYIRQGSLSAKPPESEGFGDRLFLKQRMSLLSQMTSSPTDCLFKHIASGNQKEVERLLSQEDHDKDTVQKMCHPLCFCDDCEKLVSGRLNDPSVVTPFSRDDRGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKASAEVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKETPLKCALNSKILSVMEAYHLSFERRQKSSEAPVQSPQRSVDSISQESSTSSFSSMSASSRQEETKKDYREVEKLLRAVADGDLEMVRYLLEWTEEDLEDAEDTVSAADPEFCHPLCQCPKCAPAQKRLAKVPASGLGVNVTSQDGSSPLHVAALHGRADLIPLLLKHGANAGARNADQAVPLHLACQQGHFQVVKCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEKHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNSKIMELLQVVPSCVASLDDVAETDRKEYVTVKIRKKWNSKLYDLPDEPFTRQFYFVHSAGQFKGKTSREIMARDRSVPNLTEGSLHEPGRQSVTLRQNNLPAQSGSHAAEKGNSDWPERPGLTQTGPGHRRMLRRHTVEDAVVSQGPEAAGPLSTPQEVSASRS

Target ORF information:

RefSeq Version	NM_032139.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 27 (ANKRD27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_032139.3

ORF Insert Sequence:

ATGGCTCTGT ATGATGAAGA CCTCCTGAAA AATCCTTTCT ATCTGGCTCT GCAAAAGTGC 
CGCCCTGACT TGTGCAGCAA AGTGGCCCAA ATCCATGGCA TTGTCTTAGT ACCCTGCAAA 
GGAAGCCTGT CGAGCAGCAT CCAGTCTACT TGTCAGTTTG AGTCCTACAT TTTGATACCT 
GTGGAAGAGC ATTTTCAGAC CTTAAATGGA AAGGATGTCT TTATTCAAGG GAACAGGATT 
AAATTAGGAG CTGGTTTTGC CTGTCTTCTC TCAGTGCCCA TTCTCTTTGA AGAAACTTTC 
TACAATGAAA AAGAAGAGAG TTTCAGCATC CTGTGTATAG CCCATCCTTT GGAAAAGAGA 
GAGAGTTCAG AAGAGCCTTT GGCACCCTCA GATCCCTTTT CCCTGAAAAC CATTGAAGAT 
GTGAGAGAGT TCTTGGGAAG ACACTCCGAG CGATTTGACA GGAACATCGC CTCTTTCCAT 
CGAACATTCC GAGAATGCGA GAGAAAGAGC CTCCGTCACC ACATAGACTC AGCGAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTG AGGGACTCTC ACCTGAAAAT GCTCGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTGGAGA TATACGTCCA TCATGAAATT 
TACAACCTGA TCTTTAAATA CGTGGGGACC ATGGAGGCAA GTGAGGATGC GGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTTCAG CAGAAAGATA TTGGTGTGAA ACCGGAGTTC 
AGCTTTAACA TACCTCGTGC CAAAAGAGAG CTGGCTCAGC TGAACAAATG CACCTCCCCA 
CAGCAGAAGC TTGTCTGCTT GCGAAAAGTG GTGCAGCTCA TTACACAGTC TCCAAGCCAG 
AGAGTGAACC TGGAGACCAT GTGTGCTGAT GATCTGCTAT CAGTCCTGTT ATACTTGCTT 
GTGAAAACGG AGATCCCTAA TTGGATGGCA AATTTGAGTT ACATCAAAAA CTTCAGGTTT 
AGCAGCTTGG CAAAGGATGA ACTGGGATAC TGCCTGACCT CATTCGAAGC TGCCATTGAA 
TATATTCGGC AAGGAAGCCT CTCTGCTAAA CCCCCTGAGT CTGAGGGATT TGGAGACAGG 
CTGTTCCTTA AGCAGAGAAT GAGCTTACTC TCTCAGATGA CTTCGTCTCC CACCGACTGC 
CTGTTTAAGC ACATTGCATC AGGTAACCAG AAAGAAGTGG AGAGACTTCT GAGCCAAGAG 
GACCATGATA AAGATACCGT CCAAAAGATG TGTCACCCTC TCTGCTTCTG CGATGACTGT 
GAGAAACTCG TCTCTGGGAG GTTGAATGAT CCCTCAGTTG TCACTCCATT CTCCAGAGAC 
GACAGGGGGC ACACCCCTCT CCATGTGGCT GCTGTCTGTG GGCAGGCATC CCTCATCGAC 
CTCCTGGTTT CCAAGGGCGC CATGGTAAAT GCCACAGACT ACCATGGAGC CACTCCGCTC 
CACCTGGCCT GTCAGAAGGG CTACCAGAGC GTGACGCTGC TGCTGCTGCA CTACAAGGCC 
AGCGCGGAAG TGCAGGACAA CAATGGGAAT ACGCCACTCC ACCTGGCCTG CACCTACGGC 
CACGAGGACT GTGTGAAGGC TCTGGTTTAC TACGACGTGG AGTCGTGCAG ACTTGACATT 
GGCAATGAGA AAGGAGACAC CCCTCTACAC ATTGCTGCCC GCTGGGGCTA CCAAGGCGTC 
ATAGAGACAT TGCTGCAGAA CGGAGCGTCC ACCGAGATCC AGAACAGACT GAAGGAGACG 
CCCCTCAAGT GTGCATTAAA CTCAAAGATT CTGTCTGTAA TGGAAGCCTA TCACCTGTCC 
TTCGAGAGGA GGCAGAAGTC GTCCGAGGCC CCTGTGCAGT CCCCGCAGCG CTCCGTGGAC 
TCCATCAGCC AAGAGTCCTC CACTTCCAGC TTCTCCTCCA TGTCAGCCAG CTCAAGGCAG 
GAGGAGACCA AGAAGGACTA CAGAGAGGTA GAAAAACTTT TGAGAGCAGT TGCTGATGGA 
GATCTAGAAA TGGTGCGTTA CCTGTTGGAA TGGACAGAGG AGGACCTGGA GGATGCGGAG 
GACACTGTCA GTGCAGCGGA CCCCGAATTC TGTCACCCGT TGTGCCAGTG CCCCAAGTGT 
GCCCCAGCTC AGAAGAGGCT GGCGAAGGTT CCTGCCAGTG GGCTTGGTGT GAACGTGACC 
AGCCAGGACG GCTCCTCCCC GCTGCATGTC GCCGCCCTGC ACGGCCGGGC GGACCTCATC 
CCCCTCCTGC TGAAGCACGG GGCCAACGCA GGTGCCAGGA ACGCAGACCA AGCCGTCCCG 
CTCCACCTGG CCTGCCAGCA GGGCCACTTT CAGGTGGTGA AGTGTCTGTT AGATTCGAAT 
GCAAAACCCA ATAAGAAGGA CCTCAGTGGA AACACGCCCC TCATTTACGC CTGCTCCGGT 
GGCCATCACG AGCTTGTGGC ACTGCTGCTA CAGCACGGGG CCTCCATTAA CGCTTCTAAC 
AATAAGGGCA ACACAGCGCT GCACGAGGCT GTGATTGAAA AGCACGTCTT CGTGGTAGAG 
CTGCTTCTGC TCCACGGAGC GTCAGTTCAG GTGCTGAACA AGCGGCAGCG CACGGCTGTA 
GACTGTGCTG AACAGAATTC AAAAATAATG GAATTGCTTC AGGTGGTACC AAGCTGTGTT 
GCTTCATTAG ATGATGTGGC TGAAACTGAC CGCAAGGAGT ATGTCACTGT TAAGATCAGG 
AAAAAATGGA ACTCAAAACT GTATGATCTA CCAGATGAGC CTTTTACAAG ACAGTTTTAC 
TTTGTCCACT CAGCTGGTCA GTTTAAGGGA AAGACTTCAA GGGAGATTAT GGCAAGAGAT 
AGAAGTGTCC CTAATTTAAC CGAAGGTTCT TTGCATGAGC CAGGGAGGCA AAGTGTCACA 
CTGAGACAGA ATAACCTGCC AGCTCAGAGT GGATCTCATG CTGCTGAGAA AGGCAACAGC 
GACTGGCCAG AGAGGCCTGG ACTGACACAG ACTGGCCCTG GACACAGACG GATGCTGCGG 
AGACACACGG TAGAGGATGC GGTCGTGTCC CAGGGCCCGG AGGCTGCTGG CCCCCTCTCC 
ACTCCCCAAG AGGTTAGTGC TTCCCGGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD27 ( XM_006723412.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006723475.1 Homo sapiens ankyrin repeat domain 27 (ANKRD27), transcript variant X1, mRNA.

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CloneID	OHu04857
Clone ID Related Accession (Same CDS sequence)	XM_006723412.2 , NM_032139.2 , NM_032139.3
Accession Version	XM_006723412.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006723412.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGT ATGATGAAGA CCTCCTGAAA AATCCTTTCT ATCTGGCTCT GCAAAAGTGC 
CGCCCTGACT TGTGCAGCAA AGTGGCCCAA ATCCATGGCA TTGTCTTAGT ACCCTGCAAA 
GGAAGCCTGT CGAGCAGCAT CCAGTCTACT TGTCAGTTTG AGTCCTACAT TTTGATACCT 
GTGGAAGAGC ATTTTCAGAC CTTAAATGGA AAGGATGTCT TTATTCAAGG GAACAGGATT 
AAATTAGGAG CTGGTTTTGC CTGTCTTCTC TCAGTGCCCA TTCTCTTTGA AGAAACTTTC 
TACAATGAAA AAGAAGAGAG TTTCAGCATC CTGTGTATAG CCCATCCTTT GGAAAAGAGA 
GAGAGTTCAG AAGAGCCTTT GGCACCCTCA GATCCCTTTT CCCTGAAAAC CATTGAAGAT 
GTGAGAGAGT TCTTGGGAAG ACACTCCGAG CGATTTGACA GGAACATCGC CTCTTTCCAT 
CGAACATTCC GAGAATGCGA GAGAAAGAGC CTCCGTCACC ACATAGACTC AGCGAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTG AGGGACTCTC ACCTGAAAAT GCTCGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTGGAGA TATACGTCCA TCATGAAATT 
TACAACCTGA TCTTTAAATA CGTGGGGACC ATGGAGGCAA GTGAGGATGC GGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTTCAG CAGAAAGATA TTGGTGTGAA ACCGGAGTTC 
AGCTTTAACA TACCTCGTGC CAAAAGAGAG CTGGCTCAGC TGAACAAATG CACCTCCCCA 
CAGCAGAAGC TTGTCTGCTT GCGAAAAGTG GTGCAGCTCA TTACACAGTC TCCAAGCCAG 
AGAGTGAACC TGGAGACCAT GTGTGCTGAT GATCTGCTAT CAGTCCTGTT ATACTTGCTT 
GTGAAAACGG AGATCCCTAA TTGGATGGCA AATTTGAGTT ACATCAAAAA CTTCAGGTTT 
AGCAGCTTGG CAAAGGATGA ACTGGGATAC TGCCTGACCT CATTCGAAGC TGCCATTGAA 
TATATTCGGC AAGGAAGCCT CTCTGCTAAA CCCCCTGAGT CTGAGGGATT TGGAGACAGG 
CTGTTCCTTA AGCAGAGAAT GAGCTTACTC TCTCAGATGA CTTCGTCTCC CACCGACTGC 
CTGTTTAAGC ACATTGCATC AGGTAACCAG AAAGAAGTGG AGAGACTTCT GAGCCAAGAG 
GACCATGATA AAGATACCGT CCAAAAGATG TGTCACCCTC TCTGCTTCTG CGATGACTGT 
GAGAAACTCG TCTCTGGGAG GTTGAATGAT CCCTCAGTTG TCACTCCATT CTCCAGAGAC 
GACAGGGGGC ACACCCCTCT CCATGTGGCT GCTGTCTGTG GGCAGGCATC CCTCATCGAC 
CTCCTGGTTT CCAAGGGCGC CATGGTAAAT GCCACAGACT ACCATGGAGC CACTCCGCTC 
CACCTGGCCT GTCAGAAGGG CTACCAGAGC GTGACGCTGC TGCTGCTGCA CTACAAGGCC 
AGCGCGGAAG TGCAGGACAA CAATGGGAAT ACGCCACTCC ACCTGGCCTG CACCTACGGC 
CACGAGGACT GTGTGAAGGC TCTGGTTTAC TACGACGTGG AGTCGTGCAG ACTTGACATT 
GGCAATGAGA AAGGAGACAC CCCTCTACAC ATTGCTGCCC GCTGGGGCTA CCAAGGCGTC 
ATAGAGACAT TGCTGCAGAA CGGAGCGTCC ACCGAGATCC AGAACAGACT GAAGGAGACG 
CCCCTCAAGT GTGCATTAAA CTCAAAGATT CTGTCTGTAA TGGAAGCCTA TCACCTGTCC 
TTCGAGAGGA GGCAGAAGTC GTCCGAGGCC CCTGTGCAGT CCCCGCAGCG CTCCGTGGAC 
TCCATCAGCC AAGAGTCCTC CACTTCCAGC TTCTCCTCCA TGTCAGCCAG CTCAAGGCAG 
GAGGAGACCA AGAAGGACTA CAGAGAGGTA GAAAAACTTT TGAGAGCAGT TGCTGATGGA 
GATCTAGAAA TGGTGCGTTA CCTGTTGGAA TGGACAGAGG AGGACCTGGA GGATGCGGAG 
GACACTGTCA GTGCAGCGGA CCCCGAATTC TGTCACCCGT TGTGCCAGTG CCCCAAGTGT 
GCCCCAGCTC AGAAGAGGCT GGCGAAGGTT CCTGCCAGTG GGCTTGGTGT GAACGTGACC 
AGCCAGGACG GCTCCTCCCC GCTGCATGTC GCCGCCCTGC ACGGCCGGGC GGACCTCATC 
CCCCTCCTGC TGAAGCACGG GGCCAACGCA GGTGCCAGGA ACGCAGACCA AGCCGTCCCG 
CTCCACCTGG CCTGCCAGCA GGGCCACTTT CAGGTGGTGA AGTGTCTGTT AGATTCGAAT 
GCAAAACCCA ATAAGAAGGA CCTCAGTGGA AACACGCCCC TCATTTACGC CTGCTCCGGT 
GGCCATCACG AGCTTGTGGC ACTGCTGCTA CAGCACGGGG CCTCCATTAA CGCTTCTAAC 
AATAAGGGCA ACACAGCGCT GCACGAGGCT GTGATTGAAA AGCACGTCTT CGTGGTAGAG 
CTGCTTCTGC TCCACGGAGC GTCAGTTCAG GTGCTGAACA AGCGGCAGCG CACGGCTGTA 
GACTGTGCTG AACAGAATTC AAAAATAATG GAATTGCTTC AGGTGGTACC AAGCTGTGTT 
GCTTCATTAG ATGATGTGGC TGAAACTGAC CGCAAGGAGT ATGTCACTGT TAAGATCAGG 
AAAAAATGGA ACTCAAAACT GTATGATCTA CCAGATGAGC CTTTTACAAG ACAGTTTTAC 
TTTGTCCACT CAGCTGGTCA GTTTAAGGGA AAGACTTCAA GGGAGATTAT GGCAAGAGAT 
AGAAGTGTCC CTAATTTAAC CGAAGGTTCT TTGCATGAGC CAGGGAGGCA AAGTGTCACA 
CTGAGACAGA ATAACCTGCC AGCTCAGAGT GGATCTCATG CTGCTGAGAA AGGCAACAGC 
GACTGGCCAG AGAGGCCTGG ACTGACACAG ACTGGCCCTG GACACAGACG GATGCTGCGG 
AGACACACGG TAGAGGATGC GGTCGTGTCC CAGGGCCCGG AGGCTGCTGG CCCCCTCTCC 
ACTCCCCAAG AGGTTAGTGC TTCCCGGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006723475.1
CDS	50..3202
Translation	MALYDEDLLKNPFYLALQKCRPDLCSKVAQIHGIVLVPCKGSLSSSIQSTCQFESYILIPVEEHFQTLNGKDVFIQGNRIKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLEKRESSEEPLAPSDPFSLKTIEDVREFLGRHSERFDRNIASFHRTFRECERKSLRHHIDSANALYTKCLQQLLRDSHLKMLAKQEAQMNLMKQAVEIYVHHEIYNLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELAQLNKCTSPQQKLVCLRKVVQLITQSPSQRVNLETMCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSLAKDELGYCLTSFEAAIEYIRQGSLSAKPPESEGFGDRLFLKQRMSLLSQMTSSPTDCLFKHIASGNQKEVERLLSQEDHDKDTVQKMCHPLCFCDDCEKLVSGRLNDPSVVTPFSRDDRGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKASAEVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKETPLKCALNSKILSVMEAYHLSFERRQKSSEAPVQSPQRSVDSISQESSTSSFSSMSASSRQEETKKDYREVEKLLRAVADGDLEMVRYLLEWTEEDLEDAEDTVSAADPEFCHPLCQCPKCAPAQKRLAKVPASGLGVNVTSQDGSSPLHVAALHGRADLIPLLLKHGANAGARNADQAVPLHLACQQGHFQVVKCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEKHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNSKIMELLQVVPSCVASLDDVAETDRKEYVTVKIRKKWNSKLYDLPDEPFTRQFYFVHSAGQFKGKTSREIMARDRSVPNLTEGSLHEPGRQSVTLRQNNLPAQSGSHAAEKGNSDWPERPGLTQTGPGHRRMLRRHTVEDAVVSQGPEAAGPLSTPQEVSASRS

Target ORF information:

RefSeq Version	XM_006723412.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 27 (ANKRD27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006723412.2

ORF Insert Sequence:

ATGGCTCTGT ATGATGAAGA CCTCCTGAAA AATCCTTTCT ATCTGGCTCT GCAAAAGTGC 
CGCCCTGACT TGTGCAGCAA AGTGGCCCAA ATCCATGGCA TTGTCTTAGT ACCCTGCAAA 
GGAAGCCTGT CGAGCAGCAT CCAGTCTACT TGTCAGTTTG AGTCCTACAT TTTGATACCT 
GTGGAAGAGC ATTTTCAGAC CTTAAATGGA AAGGATGTCT TTATTCAAGG GAACAGGATT 
AAATTAGGAG CTGGTTTTGC CTGTCTTCTC TCAGTGCCCA TTCTCTTTGA AGAAACTTTC 
TACAATGAAA AAGAAGAGAG TTTCAGCATC CTGTGTATAG CCCATCCTTT GGAAAAGAGA 
GAGAGTTCAG AAGAGCCTTT GGCACCCTCA GATCCCTTTT CCCTGAAAAC CATTGAAGAT 
GTGAGAGAGT TCTTGGGAAG ACACTCCGAG CGATTTGACA GGAACATCGC CTCTTTCCAT 
CGAACATTCC GAGAATGCGA GAGAAAGAGC CTCCGTCACC ACATAGACTC AGCGAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTG AGGGACTCTC ACCTGAAAAT GCTCGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTGGAGA TATACGTCCA TCATGAAATT 
TACAACCTGA TCTTTAAATA CGTGGGGACC ATGGAGGCAA GTGAGGATGC GGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTTCAG CAGAAAGATA TTGGTGTGAA ACCGGAGTTC 
AGCTTTAACA TACCTCGTGC CAAAAGAGAG CTGGCTCAGC TGAACAAATG CACCTCCCCA 
CAGCAGAAGC TTGTCTGCTT GCGAAAAGTG GTGCAGCTCA TTACACAGTC TCCAAGCCAG 
AGAGTGAACC TGGAGACCAT GTGTGCTGAT GATCTGCTAT CAGTCCTGTT ATACTTGCTT 
GTGAAAACGG AGATCCCTAA TTGGATGGCA AATTTGAGTT ACATCAAAAA CTTCAGGTTT 
AGCAGCTTGG CAAAGGATGA ACTGGGATAC TGCCTGACCT CATTCGAAGC TGCCATTGAA 
TATATTCGGC AAGGAAGCCT CTCTGCTAAA CCCCCTGAGT CTGAGGGATT TGGAGACAGG 
CTGTTCCTTA AGCAGAGAAT GAGCTTACTC TCTCAGATGA CTTCGTCTCC CACCGACTGC 
CTGTTTAAGC ACATTGCATC AGGTAACCAG AAAGAAGTGG AGAGACTTCT GAGCCAAGAG 
GACCATGATA AAGATACCGT CCAAAAGATG TGTCACCCTC TCTGCTTCTG CGATGACTGT 
GAGAAACTCG TCTCTGGGAG GTTGAATGAT CCCTCAGTTG TCACTCCATT CTCCAGAGAC 
GACAGGGGGC ACACCCCTCT CCATGTGGCT GCTGTCTGTG GGCAGGCATC CCTCATCGAC 
CTCCTGGTTT CCAAGGGCGC CATGGTAAAT GCCACAGACT ACCATGGAGC CACTCCGCTC 
CACCTGGCCT GTCAGAAGGG CTACCAGAGC GTGACGCTGC TGCTGCTGCA CTACAAGGCC 
AGCGCGGAAG TGCAGGACAA CAATGGGAAT ACGCCACTCC ACCTGGCCTG CACCTACGGC 
CACGAGGACT GTGTGAAGGC TCTGGTTTAC TACGACGTGG AGTCGTGCAG ACTTGACATT 
GGCAATGAGA AAGGAGACAC CCCTCTACAC ATTGCTGCCC GCTGGGGCTA CCAAGGCGTC 
ATAGAGACAT TGCTGCAGAA CGGAGCGTCC ACCGAGATCC AGAACAGACT GAAGGAGACG 
CCCCTCAAGT GTGCATTAAA CTCAAAGATT CTGTCTGTAA TGGAAGCCTA TCACCTGTCC 
TTCGAGAGGA GGCAGAAGTC GTCCGAGGCC CCTGTGCAGT CCCCGCAGCG CTCCGTGGAC 
TCCATCAGCC AAGAGTCCTC CACTTCCAGC TTCTCCTCCA TGTCAGCCAG CTCAAGGCAG 
GAGGAGACCA AGAAGGACTA CAGAGAGGTA GAAAAACTTT TGAGAGCAGT TGCTGATGGA 
GATCTAGAAA TGGTGCGTTA CCTGTTGGAA TGGACAGAGG AGGACCTGGA GGATGCGGAG 
GACACTGTCA GTGCAGCGGA CCCCGAATTC TGTCACCCGT TGTGCCAGTG CCCCAAGTGT 
GCCCCAGCTC AGAAGAGGCT GGCGAAGGTT CCTGCCAGTG GGCTTGGTGT GAACGTGACC 
AGCCAGGACG GCTCCTCCCC GCTGCATGTC GCCGCCCTGC ACGGCCGGGC GGACCTCATC 
CCCCTCCTGC TGAAGCACGG GGCCAACGCA GGTGCCAGGA ACGCAGACCA AGCCGTCCCG 
CTCCACCTGG CCTGCCAGCA GGGCCACTTT CAGGTGGTGA AGTGTCTGTT AGATTCGAAT 
GCAAAACCCA ATAAGAAGGA CCTCAGTGGA AACACGCCCC TCATTTACGC CTGCTCCGGT 
GGCCATCACG AGCTTGTGGC ACTGCTGCTA CAGCACGGGG CCTCCATTAA CGCTTCTAAC 
AATAAGGGCA ACACAGCGCT GCACGAGGCT GTGATTGAAA AGCACGTCTT CGTGGTAGAG 
CTGCTTCTGC TCCACGGAGC GTCAGTTCAG GTGCTGAACA AGCGGCAGCG CACGGCTGTA 
GACTGTGCTG AACAGAATTC AAAAATAATG GAATTGCTTC AGGTGGTACC AAGCTGTGTT 
GCTTCATTAG ATGATGTGGC TGAAACTGAC CGCAAGGAGT ATGTCACTGT TAAGATCAGG 
AAAAAATGGA ACTCAAAACT GTATGATCTA CCAGATGAGC CTTTTACAAG ACAGTTTTAC 
TTTGTCCACT CAGCTGGTCA GTTTAAGGGA AAGACTTCAA GGGAGATTAT GGCAAGAGAT 
AGAAGTGTCC CTAATTTAAC CGAAGGTTCT TTGCATGAGC CAGGGAGGCA AAGTGTCACA 
CTGAGACAGA ATAACCTGCC AGCTCAGAGT GGATCTCATG CTGCTGAGAA AGGCAACAGC 
GACTGGCCAG AGAGGCCTGG ACTGACACAG ACTGGCCCTG GACACAGACG GATGCTGCGG 
AGACACACGG TAGAGGATGC GGTCGTGTCC CAGGGCCCGG AGGCTGCTGG CCCCCTCTCC 
ACTCCCCAAG AGGTTAGTGC TTCCCGGTCC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKRD27 ( XM_017027349.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882838.1 Homo sapiens ankyrin repeat domain 27 (ANKRD27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu52519
Clone ID Related Accession (Same CDS sequence)	XM_017027349.1
Accession Version	XM_017027349.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat domain-containing protein 27 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTCGCCA AGCAGGAGGC CCAGATGAAC CTGATGAAGC AGGCAGTGGA GATATACGTC 
CATCATGAAA TTTACAACCT GATCTTTAAA TACGTGGGGA CCATGGAGGC AAGTGAGGAT 
GCGGCCTTTA ACAAAATCAC AAGAAGCCTT CAAGATCTTC AGCAGAAAGA TATTGGTGTG 
AAACCGGAGT TCAGCTTTAA CATACCTCGT GCCAAAAGAG AGCTGGCTCA GCTGAACAAA 
TGCACCTCCC CACAGCAGAA GCTTGTCTGC TTGCGAAAAG TGGTGCAGCT CATTACACAG 
TCTCCAAGCC AGAGAGTGAA CCTGGAGACC ATGTGTGCTG ATGATCTGCT ATCAGTCCTG 
TTATACTTGC TTGTGAAAAC GGAGATCCCT AATTGGATGG CAAATTTGAG TTACATCAAA 
AACTTCAGGT TTAGCAGCTT GGCAAAGGAT GAACTGGGAT ACTGCCTGAC CTCATTCGAA 
GCTGCCATTG AATATATTCG GCAAGGAAGC CTCTCTGCTA AACCCCCTGA GTCTGAGGGA 
TTTGGAGACA GGCTGTTCCT TAAGCAGAGA ATGAGCTTAC TCTCTCAGAT GACTTCGTCT 
CCCACCGACT GCCTGTTTAA GCACATTGCA TCAGGTAACC AGAAAGAAGT GGAGAGACTT 
CTGAGCCAAG AGGACCATGA TAAAGATACC GTCCAAAAGA TGTGTCACCC TCTCTGCTTC 
TGCGATGACT GTGAGAAACT CGTCTCTGGG AGGTTGAATG ATCCCTCAGT TGTCACTCCA 
TTCTCCAGAG ACGACAGGGG GCACACCCCT CTCCATGTGG CTGCTGTCTG TGGGCAGGCA 
TCCCTCATCG ACCTCCTGGT TTCCAAGGGC GCCATGGTAA ATGCCACAGA CTACCATGGA 
GCCACTCCGC TCCACCTGGC CTGTCAGAAG GGCTACCAGA GCGTGACGCT GCTGCTGCTG 
CACTACAAGG CCAGCGCGGA AGTGCAGGAC AACAATGGGA ATACGCCACT CCACCTGGCC 
TGCACCTACG GCCACGAGGA CTGTGTGAAG GCTCTGGTTT ACTACGACGT GGAGTCGTGC 
AGACTTGACA TTGGCAATGA GAAAGGAGAC ACCCCTCTAC ACATTGCTGC CCGCTGGGGC 
TACCAAGGCG TCATAGAGAC ATTGCTGCAG AACGGAGCGT CCACCGAGAT CCAGAACAGA 
CTGAAGGAGA CGCCCCTCAA GTGTGCATTA AACTCAAAGA TTCTGTCTGT AATGGAAGCC 
TATCACCTGT CCTTCGAGAG GAGGCAGAAG TCGTCCGAGG CCCCTGTGCA GTCCCCGCAG 
CGCTCCGTGG ACTCCATCAG CCAAGAGTCC TCCACTTCCA GCTTCTCCTC CATGTCAGCC 
AGCTCAAGGC AGGAGGAGAC CAAGAAGGAC TACAGAGAGG TAGAAAAACT TTTGAGAGCA 
GTTGCTGATG GAGATCTAGA AATGGTGCGT TACCTGTTGG AATGGACAGA GGAGGACCTG 
GAGGATGCGG AGGACACTGT CAGTGCAGCG GACCCCGAAT TCTGTCACCC GTTGTGCCAG 
TGCCCCAAGT GTGCCCCAGC TCAGAAGAGG CTGGCGAAGG TTCCTGCCAG TGGGCTTGGT 
GTGAACGTGA CCAGCCAGGA CGGCTCCTCC CCGCTGCATG TCGCCGCCCT GCACGGCCGG 
GCGGACCTCA TCCCCCTCCT GCTGAAGCAC GGGGCCAACG CAGGTGCCAG GAACGCAGAC 
CAAGCCGTCC CGCTCCACCT GGCCTGCCAG CAGGGCCACT TTCAGGTGGT GAAGTGTCTG 
TTAGATTCGA ATGCAAAACC CAATAAGAAG GACCTCAGTG GAAACACGCC CCTCATTTAC 
GCCTGCTCCG GTGGCCATCA CGAGCTTGTG GCACTGCTGC TACAGCACGG GGCCTCCATT 
AACGCTTCTA ACAATAAGGG CAACACAGCG CTGCACGAGG CTGTGATTGA AAAGCACGTC 
TTCGTGGTAG AGCTGCTTCT GCTCCACGGA GCGTCAGTTC AGGTGCTGAA CAAGCGGCAG 
CGCACGGCTG TAGACTGTGC TGAACAGAAT TCAAAAATAA TGGAATTGCT TCAGGTGGTA 
CCAAGCTGTG TTGCTTCATT AGATGATGTG GCTGAAACTG ACCGCAAGGA GTATGTCACT 
GTTAAGATCA GGAAAAAATG GAACTCAAAA CTGTATGATC TACCAGATGA GCCTTTTACA 
AGACAGTTTT ACTTTGTCCA CTCAGCTGGT CAGTTTAAGG GAAAGACTTC AAGGGAGATT 
ATGGCAAGAG ATAGAAGTGT CCCTAATTTA ACCGAAGGTT CTTTGCATGA GCCAGGGAGG 
CAAAGTGTCA CACTGAGACA GAATAACCTG CCAGCTCAGA GTGGATCTCA TGCTGCTGAG 
AAAGGCAACA GCGACTGGCC AGAGAGGCCT GGACTGACAC AGACTGGCCC TGGACACAGA 
CGGATGCTGC GGAGACACAC GGTAGAGGAT GCGGTCGTGT CCCAGGGCCC GGAGGCTGCT 
GGCCCCCTCT CCACTCCCCA AGAGGTTAGT GCTTCCCGGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882838.1
CDS	607..3171
Translation	MLAKQEAQMNLMKQAVEIYVHHEIYNLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELAQLNKCTSPQQKLVCLRKVVQLITQSPSQRVNLETMCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSLAKDELGYCLTSFEAAIEYIRQGSLSAKPPESEGFGDRLFLKQRMSLLSQMTSSPTDCLFKHIASGNQKEVERLLSQEDHDKDTVQKMCHPLCFCDDCEKLVSGRLNDPSVVTPFSRDDRGHTPLHVAAVCGQASLIDLLVSKGAMVNATDYHGATPLHLACQKGYQSVTLLLLHYKASAEVQDNNGNTPLHLACTYGHEDCVKALVYYDVESCRLDIGNEKGDTPLHIAARWGYQGVIETLLQNGASTEIQNRLKETPLKCALNSKILSVMEAYHLSFERRQKSSEAPVQSPQRSVDSISQESSTSSFSSMSASSRQEETKKDYREVEKLLRAVADGDLEMVRYLLEWTEEDLEDAEDTVSAADPEFCHPLCQCPKCAPAQKRLAKVPASGLGVNVTSQDGSSPLHVAALHGRADLIPLLLKHGANAGARNADQAVPLHLACQQGHFQVVKCLLDSNAKPNKKDLSGNTPLIYACSGGHHELVALLLQHGASINASNNKGNTALHEAVIEKHVFVVELLLLHGASVQVLNKRQRTAVDCAEQNSKIMELLQVVPSCVASLDDVAETDRKEYVTVKIRKKWNSKLYDLPDEPFTRQFYFVHSAGQFKGKTSREIMARDRSVPNLTEGSLHEPGRQSVTLRQNNLPAQSGSHAAEKGNSDWPERPGLTQTGPGHRRMLRRHTVEDAVVSQGPEAAGPLSTPQEVSASRS

Target ORF information:

RefSeq Version	XM_017027349.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat domain 27 (ANKRD27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017027349.1

ORF Insert Sequence:

ATGCTCGCCA AGCAGGAGGC CCAGATGAAC CTGATGAAGC AGGCAGTGGA GATATACGTC 
CATCATGAAA TTTACAACCT GATCTTTAAA TACGTGGGGA CCATGGAGGC AAGTGAGGAT 
GCGGCCTTTA ACAAAATCAC AAGAAGCCTT CAAGATCTTC AGCAGAAAGA TATTGGTGTG 
AAACCGGAGT TCAGCTTTAA CATACCTCGT GCCAAAAGAG AGCTGGCTCA GCTGAACAAA 
TGCACCTCCC CACAGCAGAA GCTTGTCTGC TTGCGAAAAG TGGTGCAGCT CATTACACAG 
TCTCCAAGCC AGAGAGTGAA CCTGGAGACC ATGTGTGCTG ATGATCTGCT ATCAGTCCTG 
TTATACTTGC TTGTGAAAAC GGAGATCCCT AATTGGATGG CAAATTTGAG TTACATCAAA 
AACTTCAGGT TTAGCAGCTT GGCAAAGGAT GAACTGGGAT ACTGCCTGAC CTCATTCGAA 
GCTGCCATTG AATATATTCG GCAAGGAAGC CTCTCTGCTA AACCCCCTGA GTCTGAGGGA 
TTTGGAGACA GGCTGTTCCT TAAGCAGAGA ATGAGCTTAC TCTCTCAGAT GACTTCGTCT 
CCCACCGACT GCCTGTTTAA GCACATTGCA TCAGGTAACC AGAAAGAAGT GGAGAGACTT 
CTGAGCCAAG AGGACCATGA TAAAGATACC GTCCAAAAGA TGTGTCACCC TCTCTGCTTC 
TGCGATGACT GTGAGAAACT CGTCTCTGGG AGGTTGAATG ATCCCTCAGT TGTCACTCCA 
TTCTCCAGAG ACGACAGGGG GCACACCCCT CTCCATGTGG CTGCTGTCTG TGGGCAGGCA 
TCCCTCATCG ACCTCCTGGT TTCCAAGGGC GCCATGGTAA ATGCCACAGA CTACCATGGA 
GCCACTCCGC TCCACCTGGC CTGTCAGAAG GGCTACCAGA GCGTGACGCT GCTGCTGCTG 
CACTACAAGG CCAGCGCGGA AGTGCAGGAC AACAATGGGA ATACGCCACT CCACCTGGCC 
TGCACCTACG GCCACGAGGA CTGTGTGAAG GCTCTGGTTT ACTACGACGT GGAGTCGTGC 
AGACTTGACA TTGGCAATGA GAAAGGAGAC ACCCCTCTAC ACATTGCTGC CCGCTGGGGC 
TACCAAGGCG TCATAGAGAC ATTGCTGCAG AACGGAGCGT CCACCGAGAT CCAGAACAGA 
CTGAAGGAGA CGCCCCTCAA GTGTGCATTA AACTCAAAGA TTCTGTCTGT AATGGAAGCC 
TATCACCTGT CCTTCGAGAG GAGGCAGAAG TCGTCCGAGG CCCCTGTGCA GTCCCCGCAG 
CGCTCCGTGG ACTCCATCAG CCAAGAGTCC TCCACTTCCA GCTTCTCCTC CATGTCAGCC 
AGCTCAAGGC AGGAGGAGAC CAAGAAGGAC TACAGAGAGG TAGAAAAACT TTTGAGAGCA 
GTTGCTGATG GAGATCTAGA AATGGTGCGT TACCTGTTGG AATGGACAGA GGAGGACCTG 
GAGGATGCGG AGGACACTGT CAGTGCAGCG GACCCCGAAT TCTGTCACCC GTTGTGCCAG 
TGCCCCAAGT GTGCCCCAGC TCAGAAGAGG CTGGCGAAGG TTCCTGCCAG TGGGCTTGGT 
GTGAACGTGA CCAGCCAGGA CGGCTCCTCC CCGCTGCATG TCGCCGCCCT GCACGGCCGG 
GCGGACCTCA TCCCCCTCCT GCTGAAGCAC GGGGCCAACG CAGGTGCCAG GAACGCAGAC 
CAAGCCGTCC CGCTCCACCT GGCCTGCCAG CAGGGCCACT TTCAGGTGGT GAAGTGTCTG 
TTAGATTCGA ATGCAAAACC CAATAAGAAG GACCTCAGTG GAAACACGCC CCTCATTTAC 
GCCTGCTCCG GTGGCCATCA CGAGCTTGTG GCACTGCTGC TACAGCACGG GGCCTCCATT 
AACGCTTCTA ACAATAAGGG CAACACAGCG CTGCACGAGG CTGTGATTGA AAAGCACGTC 
TTCGTGGTAG AGCTGCTTCT GCTCCACGGA GCGTCAGTTC AGGTGCTGAA CAAGCGGCAG 
CGCACGGCTG TAGACTGTGC TGAACAGAAT TCAAAAATAA TGGAATTGCT TCAGGTGGTA 
CCAAGCTGTG TTGCTTCATT AGATGATGTG GCTGAAACTG ACCGCAAGGA GTATGTCACT 
GTTAAGATCA GGAAAAAATG GAACTCAAAA CTGTATGATC TACCAGATGA GCCTTTTACA 
AGACAGTTTT ACTTTGTCCA CTCAGCTGGT CAGTTTAAGG GAAAGACTTC AAGGGAGATT 
ATGGCAAGAG ATAGAAGTGT CCCTAATTTA ACCGAAGGTT CTTTGCATGA GCCAGGGAGG 
CAAAGTGTCA CACTGAGACA GAATAACCTG CCAGCTCAGA GTGGATCTCA TGCTGCTGAG 
AAAGGCAACA GCGACTGGCC AGAGAGGCCT GGACTGACAC AGACTGGCCC TGGACACAGA 
CGGATGCTGC GGAGACACAC GGTAGAGGAT GCGGTCGTGT CCCAGGGCCC GGAGGCTGCT 
GGCCCCCTCT CCACTCCCCA AGAGGTTAGT GCTTCCCGGT CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Multiple Roles of VARP in Endosomal Trafficking: Rabs, Retromer Components and R-SNARE VAMP7 Meet on VARP.
Traffic (Copenhagen, Denmark)17(7)709-19(2016 07)
Fukuda M

GWAS identifies four novel eosinophilic esophagitis loci.
Nature communications55593(2014 Nov)
Sleiman PM,Wang ML,Cianferoni A,Aceves S,Gonsalves N,Nadeau K,Bredenoord AJ,Furuta GT,Spergel JM,Hakonarson H

VARP is recruited on to endosomes by direct interaction with retromer, where together they function in export to the cell surface.
Developmental cell29(5)591-606(2014 Jun)
Hesketh GG,P?rez-Dorado I,Jackson LP,Wartosch L,Sch?fer IB,Gray SR,McCoy AJ,Zeldin OB,Garman EF,Harbour ME,Evans PR,Seaman MNJ,Luzio JP,Owen DJ

Common genetic variation and antidepressant efficacy in major depressive disorder: a meta-analysis of three genome-wide pharmacogenetic studies.
The American journal of psychiatry170(2)207-17(2013 Feb)
,,

The binding of Varp to VAMP7 traps VAMP7 in a closed, fusogenically inactive conformation.
Nature structural & molecular biology19(12)1300-9(2012 Dec)
Sch?fer IB,Hesketh GG,Bright NA,Gray SR,Pryor PR,Evans PR,Luzio JP,Owen DJ

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ANKRD27 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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