Ankrd27 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ankrd27
Entrez Gene ID	361555
Full Name	ankyrin repeat domain 27
General protein information	Preferred Names ankyrin repeat domain 27 Names ankyrin repeat domain-containing protein 27 ankyrin repeat domain 27 (VPS9 domain) ankyrin repeat domain-containing protein 27-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21

mRNA and Protein(s)

mRNA	Protein	Name
XM_017589356.1	XP_017444845.1	ankyrin repeat domain-containing protein 27 isoform X1
XM_017589360.1	XP_017444849.1	ankyrin repeat domain-containing protein 27 isoform X4
XM_017589358.1	XP_017444847.1	ankyrin repeat domain-containing protein 27 isoform X3
XM_017589357.1	XP_017444846.1	ankyrin repeat domain-containing protein 27 isoform X2
XM_017589359.1	XP_017444848.1	ankyrin repeat domain-containing protein 27 isoform X3
XM_017589355.1	XP_017444844.1	ankyrin repeat domain-containing protein 27 isoform X1
NM_001271264.1	NP_001258193.1	ankyrin repeat domain-containing protein 27

Pathways from BioSystems

Homologies

	LOC716232	XP_001106852.1
Rattus norvegicus (Norway rat)	Ankrd27	NP_001258193.1
Pan troglodytes (chimpanzee)	ANKRD27	XP_001151730.1
Bos taurus (cattle)	ANKRD27	NP_001096002.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003151	XP_001688409.2
Homo sapiens (human)	ANKRD27	NP_115515.2
Danio rerio (zebrafish)	wu:fc41e04	XP_005163257.1
Xenopus tropicalis (tropical clawed frog)	ankrd27	XP_004913610.1
Canis lupus familiaris (dog)	ANKRD27	XP_005616956.1
Mus musculus (house mouse)	Ankrd27	NP_663608.3
Gallus gallus (chicken)	ANKRD27	NP_001129150.1

Gene Ontology
Bibliography

Related articles in PubMed

Retromer and sorting nexins in endosomal sorting.
Gallon M, Cullen PJ
Biochemical Society transactions43(1)33-47(2015 Feb)

GWAS identifies four novel eosinophilic esophagitis loci.
Sleiman PM, Wang ML, Cianferoni A, Aceves S, Gonsalves N, Nadeau K, Bredenoord AJ, Furuta GT, Spergel JM, Hakonarson H
Nature communications55593(2014 Nov)

VARP is recruited on to endosomes by direct interaction with retromer, where together they function in export to the cell surface.
Hesketh GG, P?rez-Dorado I, Jackson LP, Wartosch L, Sch?fer IB, Gray SR, McCoy AJ, Zeldin OB, Garman EF, Harbour ME, Evans PR, Seaman MNJ, Luzio JP, Owen DJ
Developmental cell29(5)591-606(2014 Jun)

The Rab21-GEF activity of Varp, but not its Rab32/38 effector function, is required for dendrite formation in melanocytes.
Ohbayashi N, Yatsu A, Tamura K, Fukuda M
Molecular biology of the cell23(4)669-78(2012 Feb)

Evaluation of genotoxic potential of neurotoxin anatoxin-a with the use of umuC test.
Sieroslawska A, Rymuszka A
Neuro endocrinology letters31 Suppl 216-20(2010)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

show that TI-VAMP interacts with the Vps9 domain and ankyrin-repeat-containing protein Varp, a guanine nucleotide exchange factor of the small GTPase Rab21, through a specific domain herein called the interacting domain.
Title: Role of Varp, a Rab21 exchange factor and TI-VAMP/VAMP7 partner, in neurite growth.

The following Ankrd27 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ankrd27 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa56688	XM_017589360.1 Latest version!	Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
ORa56685	XM_017589356.1 Latest version!	Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa56685	XM_017589355.1 Latest version!	Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa56686	XM_017589357.1 Latest version!	Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa56687	XM_017589358.1 Latest version!	Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa56687	XM_017589359.1 Latest version!	Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa10789	NM_001271264.1 Latest version!	Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ankrd27 ( XM_017589360.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444849.1 Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa56688
Clone ID Related Accession (Same CDS sequence)	XM_017589360.1
Accession Version	XM_017589360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1341bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin repeat domain-containing protein 27 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGT ATGATGAAGA TCTCCTGAGA AACCCTTTCT ACCTGGCCCT TCAGAAGTGG 
CGCCCCGACT TGTGCAGTAA GGTGGCCCAG ATCCATGGCA TTGTCCTAGT GCCTTGCAGA 
GGAAGCTTGC CGAGCAACGT GCAGGCCTCC TGCCAGTTCG AGTCCTATGT TTTGGTACCC 
ATGGAAGGAC ACTTTCAGAC CTTAGATGGA AAGGCTGTCC TCATAGAAGG AAACAGGGTT 
AAGCTAGGAG CAGGCTTTGC TTGCCTTCTC TCTGTGCCCA TCCTCTTTGA AGAGACTTTC 
TACAACGAAA AGGAAGAGAG TTTCAGCATT CTCTGCATCG CTCATCCTTT GGAGAAGAGG 
GAGACATCAG AAGAACCTTC AGCACCCTCA GATCCTTTCT CTCTGAAAAC CATCAAAGAT 
GTGAGAGAAT TTTTGGGAAG ACACTCAGAG AGATTCGACA AGCACATCGC GTCTTTCCAC 
CGGACATTCC GAGAATGTGA AAGAAAGAGC CTCCGCCATC TCATAGACTC CGTAAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTC AGAGACTCTC ACCTGAAGGT TCTTGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTAGAGA TGTATGTCCA TCATGATATT 
TACGACCTGA TCTTCAAATA CGTGGGGACC ATGGAGGCGA GCGAGGATGC CGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTCCAG CAGAAAGACA TTGGCGTGAA ACCTGAGTTC 
AGCTTCAACA TTCCTCGGGC AAAGAGAGAG CTGGGACAGC TGAACAAGTG TACATCCCCA 
CAGCAGAAGC TGCTGTGTCT GCGGAAGGTG GTCCAGCTCA TGACACAGTC TCCCAGCCAG 
AGAGTGAATT TGGAGACCGT CTGTGCTGAT GATCTTCTTT CCGTCCTGTT ATATCTGCTT 
GTGAAAACAG AGATCCCTAA CTGGATGGCG AATTTGAGTT ACATCAAAAA CTTCAGATTT 
AGCAGCTCGC CCAAAGATGA GCTAGGATAC TGCCTGACCT CAGTCGAGGC TGCGATTGAA 
TACATCCGGC AAGGAAGCCT CTCTGCACAG CCGCCTGACG CTGAGGGATT TGGCGACAGA 
CTCTTTCTCA AGCAACGGAT GAACTTATTG TCCCAGATGT CATCAACACC CATCGACTGC 
CTGTTTAAGC ACATCGCTTC TGGCAACCAA AAGGAAGTAG AAAGACTTCT GAGTCAAGAT 
GACCAAGACA AAGACGCCAT GCAAAAGATG TGCCACCCGC TTTGCTCCTG TGAGGACTGT 
GAGAAGCTTG TCTCTGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444849.1
CDS	134..1474
Translation	MALYDEDLLRNPFYLALQKWRPDLCSKVAQIHGIVLVPCRGSLPSNVQASCQFESYVLVPMEGHFQTLDGKAVLIEGNRVKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLEKRETSEEPSAPSDPFSLKTIKDVREFLGRHSERFDKHIASFHRTFRECERKSLRHLIDSVNALYTKCLQQLLRDSHLKVLAKQEAQMNLMKQAVEMYVHHDIYDLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELGQLNKCTSPQQKLLCLRKVVQLMTQSPSQRVNLETVCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSSPKDELGYCLTSVEAAIEYIRQGSLSAQPPDAEGFGDRLFLKQRMNLLSQMSSTPIDCLFKHIASGNQKEVERLLSQDDQDKDAMQKMCHPLCSCEDCEKLVSG

Target ORF information:

RefSeq Version	XM_017589360.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589360.1

ORF Insert Sequence:

ATGGCTCTGT ATGATGAAGA TCTCCTGAGA AACCCTTTCT ACCTGGCCCT TCAGAAGTGG 
CGCCCCGACT TGTGCAGTAA GGTGGCCCAG ATCCATGGCA TTGTCCTAGT GCCTTGCAGA 
GGAAGCTTGC CGAGCAACGT GCAGGCCTCC TGCCAGTTCG AGTCCTATGT TTTGGTACCC 
ATGGAAGGAC ACTTTCAGAC CTTAGATGGA AAGGCTGTCC TCATAGAAGG AAACAGGGTT 
AAGCTAGGAG CAGGCTTTGC TTGCCTTCTC TCTGTGCCCA TCCTCTTTGA AGAGACTTTC 
TACAACGAAA AGGAAGAGAG TTTCAGCATT CTCTGCATCG CTCATCCTTT GGAGAAGAGG 
GAGACATCAG AAGAACCTTC AGCACCCTCA GATCCTTTCT CTCTGAAAAC CATCAAAGAT 
GTGAGAGAAT TTTTGGGAAG ACACTCAGAG AGATTCGACA AGCACATCGC GTCTTTCCAC 
CGGACATTCC GAGAATGTGA AAGAAAGAGC CTCCGCCATC TCATAGACTC CGTAAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTC AGAGACTCTC ACCTGAAGGT TCTTGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTAGAGA TGTATGTCCA TCATGATATT 
TACGACCTGA TCTTCAAATA CGTGGGGACC ATGGAGGCGA GCGAGGATGC CGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTCCAG CAGAAAGACA TTGGCGTGAA ACCTGAGTTC 
AGCTTCAACA TTCCTCGGGC AAAGAGAGAG CTGGGACAGC TGAACAAGTG TACATCCCCA 
CAGCAGAAGC TGCTGTGTCT GCGGAAGGTG GTCCAGCTCA TGACACAGTC TCCCAGCCAG 
AGAGTGAATT TGGAGACCGT CTGTGCTGAT GATCTTCTTT CCGTCCTGTT ATATCTGCTT 
GTGAAAACAG AGATCCCTAA CTGGATGGCG AATTTGAGTT ACATCAAAAA CTTCAGATTT 
AGCAGCTCGC CCAAAGATGA GCTAGGATAC TGCCTGACCT CAGTCGAGGC TGCGATTGAA 
TACATCCGGC AAGGAAGCCT CTCTGCACAG CCGCCTGACG CTGAGGGATT TGGCGACAGA 
CTCTTTCTCA AGCAACGGAT GAACTTATTG TCCCAGATGT CATCAACACC CATCGACTGC 
CTGTTTAAGC ACATCGCTTC TGGCAACCAA AAGGAAGTAG AAAGACTTCT GAGTCAAGAT 
GACCAAGACA AAGACGCCAT GCAAAAGATG TGCCACCCGC TTTGCTCCTG TGAGGACTGT 
GAGAAGCTTG TCTCTGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd27 ( XM_017589356.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444845.1 Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa56685
Clone ID Related Accession (Same CDS sequence)	XM_017589356.1 , XM_017589355.1
Accession Version	XM_017589356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin repeat domain-containing protein 27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGT ATGATGAAGA TCTCCTGAGA AACCCTTTCT ACCTGGCCCT TCAGAAGTGG 
CGCCCCGACT TGTGCAGTAA GGTGGCCCAG ATCCATGGCA TTGTCCTAGT GCCTTGCAGA 
GGAAGCTTGC CGAGCAACGT GCAGGCCTCC TGCCAGTTCG AGTCCTATGT TTTGGTACCC 
ATGGAAGGAC ACTTTCAGAC CTTAGATGGA AAGGCTGTCC TCATAGAAGG AAACAGGGTT 
AAGCTAGGAG CAGGCTTTGC TTGCCTTCTC TCTGTGCCCA TCCTCTTTGA AGAGACTTTC 
TACAACGAAA AGGAAGAGAG TTTCAGCATT CTCTGCATCG CTCATCCTTT GGAGAAGAGG 
GAGACATCAG AAGAACCTTC AGCACCCTCA GATCCTTTCT CTCTGAAAAC CATCAAAGAT 
GTGAGAGAAT TTTTGGGAAG ACACTCAGAG AGATTCGACA AGCACATCGC GTCTTTCCAC 
CGGACATTCC GAGAATGTGA AAGAAAGAGC CTCCGCCATC TCATAGACTC CGTAAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTC AGAGACTCTC ACCTGAAGGT TCTTGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTAGAGA TGTATGTCCA TCATGATATT 
TACGACCTGA TCTTCAAATA CGTGGGGACC ATGGAGGCGA GCGAGGATGC CGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTCCAG CAGAAAGACA TTGGCGTGAA ACCTGAGTTC 
AGCTTCAACA TTCCTCGGGC AAAGAGAGAG CTGGGACAGC TGAACAAGTG TACATCCCCA 
CAGCAGAAGC TGCTGTGTCT GCGGAAGGTG GTCCAGCTCA TGACACAGTC TCCCAGCCAG 
AGAGTGAATT TGGAGACCGT CTGTGCTGAT GATCTTCTTT CCGTCCTGTT ATATCTGCTT 
GTGAAAACAG AGATCCCTAA CTGGATGGCG AATTTGAGTT ACATCAAAAA CTTCAGATTT 
AGCAGCTCGC CCAAAGATGA GCTAGGATAC TGCCTGACCT CAGTCGAGGC TGCGATTGAA 
TACATCCGGC AAGGAAGCCT CTCTGCACAG CCGCCTGACG CTGAGGGATT TGGCGACAGA 
CTCTTTCTCA AGCAACGGAT GAACTTATTG TCCCAGATGT CATCAACACC CATCGACTGC 
CTGTTTAAGC ACATCGCTTC TGGCAACCAA AAGGAAGTAG AAAGACTTCT GAGTCAAGAT 
GACCAAGACA AAGACGCCAT GCAAAAGATG TGCCACCCGC TTTGCTCCTG TGAGGACTGT 
GAGAAGCTTG TCTCTGGAAG GCTGAATGAT CCGTCGGTTG TCACTCCTTT CTCCAGAGAT 
GACAGAGGTC AAACACCCCT CCATGTGGCT GCTCTCTGTG GTCAGGCATC CCTCATTGAC 
TTCCTGGTCT CCAAAGGTGC CGTAGTGAAC GCCACAGACT ATCATGGGTC TACTCCCTTA 
CATCTGGCAT GTCAGAAAGG CTTCCAAAGT GTGACGCTGC TGCTGCTACA CTACAAGGCC 
AACACTGAGG TGCAAGACAA CAACGGCAAC ACCCCGCTGC ACCTGGCCTG CACCTACGGG 
CAGGAGGATT GTGTGAAGGC CCTGGTCTAC TATGATGTCC AGGCCTGCAG ATTGGATATT 
GGTAACGAGA AAGGAGACAC AGCCCTGCAC ATTGCAGCTC GCTGGGGTTA CGAGGGCATC 
ATAGAGACGC TGCTGCAGAA TGGAGCCCCA ACAGCAATTC AGAACAGACT GAAGGAAACA 
CCACTCAAGT GTGCACTGAA CTCGAAGATT CTGTCTGCCA TGGAAGCGTA TCATCTATCC 
TCTGACAGGA GGCCAAAGCC TTCTGAGGTC CCTGCACCGT CCCCTACACG CTCTGCGGAT 
TCCATCAGCC AGGGCTCCTC TACCTCCAGC TTTTCTTCGG TATCGGTGAG CTTCAGGCCA 
GAAGAGGTCA AAAAGGACTA CAGGGAGGTA GAAAAGCTTC TCAGAGCAGT TGCCGATGGC 
GACCTAGAAA TGGTGCGATA CCTTTTGGAG TGGACGGAGG ACGACCTAGA CGATATGGAC 
GAAGCCATCA GCACGGTGGA TCTTGAATTC TGTCACCCTT TGTGCCAGTG CCCCAAGTGT 
GCTCCAGCCC AGAAGAAACT GGCAAGGATC CCTGCCAATG GGCTCAGTGT GAATGTGACC 
AACCAGGACG GCTTCTCTCC CCTGCATATG GCTGCCCTGC ATGGCAGGAC AGACCTTGTC 
CCCCTCCTGC TGAAGCATGG AGCCTACTCA GGTGCCAGAA ACACAAGCCA AGCTGTCCCG 
CTCCACCTGG CCTGCCAACA GGGCCACTTT CAGGTGGCAA AATGTCTCTT AGACTCCAAT 
GCAAAACCTA ATAAAAGGGA CCTCAGTGGG AACACGCCCC TCATTTGCGC CTGCTCTGCC 
GGCCATCATG AAGTCGCTGC ACTGTTGCTA CAGCACGGGG CCGCCATCAA TGCTTGCAAC 
AACAAAGGCA ACACGGCCCT GCACGAGGCC GTGATGGGAA GGCACGTCTT GGTGGTGGAA 
CTGCTTCTGT TTTATGGCGC ATCTGTTGAC ATCCTGAACA AGAGGCAGTA CACGGCGGTG 
GACTGTGCAG AGCAGGACTC AAAGATAATG GAGTTGCTGC AAGTCGTCCC AAGTTGCGTT 
GCTGCTTCTC CAAACAACGT CGTTGTCGTC CAGGAGACAG AACACGAGGA TTATGTGACT 
GTGGAGATCA GGAAAAAATG TAAGTGCTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444845.1
CDS	50..2842
Translation	MALYDEDLLRNPFYLALQKWRPDLCSKVAQIHGIVLVPCRGSLPSNVQASCQFESYVLVPMEGHFQTLDGKAVLIEGNRVKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLEKRETSEEPSAPSDPFSLKTIKDVREFLGRHSERFDKHIASFHRTFRECERKSLRHLIDSVNALYTKCLQQLLRDSHLKVLAKQEAQMNLMKQAVEMYVHHDIYDLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELGQLNKCTSPQQKLLCLRKVVQLMTQSPSQRVNLETVCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSSPKDELGYCLTSVEAAIEYIRQGSLSAQPPDAEGFGDRLFLKQRMNLLSQMSSTPIDCLFKHIASGNQKEVERLLSQDDQDKDAMQKMCHPLCSCEDCEKLVSGRLNDPSVVTPFSRDDRGQTPLHVAALCGQASLIDFLVSKGAVVNATDYHGSTPLHLACQKGFQSVTLLLLHYKANTEVQDNNGNTPLHLACTYGQEDCVKALVYYDVQACRLDIGNEKGDTALHIAARWGYEGIIETLLQNGAPTAIQNRLKETPLKCALNSKILSAMEAYHLSSDRRPKPSEVPAPSPTRSADSISQGSSTSSFSSVSVSFRPEEVKKDYREVEKLLRAVADGDLEMVRYLLEWTEDDLDDMDEAISTVDLEFCHPLCQCPKCAPAQKKLARIPANGLSVNVTNQDGFSPLHMAALHGRTDLVPLLLKHGAYSGARNTSQAVPLHLACQQGHFQVAKCLLDSNAKPNKRDLSGNTPLICACSAGHHEVAALLLQHGAAINACNNKGNTALHEAVMGRHVLVVELLLFYGASVDILNKRQYTAVDCAEQDSKIMELLQVVPSCVAASPNNVVVVQETEHEDYVTVEIRKKCKCF

Target ORF information:

RefSeq Version	XM_017589356.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589356.1

ORF Insert Sequence:

ATGGCTCTGT ATGATGAAGA TCTCCTGAGA AACCCTTTCT ACCTGGCCCT TCAGAAGTGG 
CGCCCCGACT TGTGCAGTAA GGTGGCCCAG ATCCATGGCA TTGTCCTAGT GCCTTGCAGA 
GGAAGCTTGC CGAGCAACGT GCAGGCCTCC TGCCAGTTCG AGTCCTATGT TTTGGTACCC 
ATGGAAGGAC ACTTTCAGAC CTTAGATGGA AAGGCTGTCC TCATAGAAGG AAACAGGGTT 
AAGCTAGGAG CAGGCTTTGC TTGCCTTCTC TCTGTGCCCA TCCTCTTTGA AGAGACTTTC 
TACAACGAAA AGGAAGAGAG TTTCAGCATT CTCTGCATCG CTCATCCTTT GGAGAAGAGG 
GAGACATCAG AAGAACCTTC AGCACCCTCA GATCCTTTCT CTCTGAAAAC CATCAAAGAT 
GTGAGAGAAT TTTTGGGAAG ACACTCAGAG AGATTCGACA AGCACATCGC GTCTTTCCAC 
CGGACATTCC GAGAATGTGA AAGAAAGAGC CTCCGCCATC TCATAGACTC CGTAAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTC AGAGACTCTC ACCTGAAGGT TCTTGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTAGAGA TGTATGTCCA TCATGATATT 
TACGACCTGA TCTTCAAATA CGTGGGGACC ATGGAGGCGA GCGAGGATGC CGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTCCAG CAGAAAGACA TTGGCGTGAA ACCTGAGTTC 
AGCTTCAACA TTCCTCGGGC AAAGAGAGAG CTGGGACAGC TGAACAAGTG TACATCCCCA 
CAGCAGAAGC TGCTGTGTCT GCGGAAGGTG GTCCAGCTCA TGACACAGTC TCCCAGCCAG 
AGAGTGAATT TGGAGACCGT CTGTGCTGAT GATCTTCTTT CCGTCCTGTT ATATCTGCTT 
GTGAAAACAG AGATCCCTAA CTGGATGGCG AATTTGAGTT ACATCAAAAA CTTCAGATTT 
AGCAGCTCGC CCAAAGATGA GCTAGGATAC TGCCTGACCT CAGTCGAGGC TGCGATTGAA 
TACATCCGGC AAGGAAGCCT CTCTGCACAG CCGCCTGACG CTGAGGGATT TGGCGACAGA 
CTCTTTCTCA AGCAACGGAT GAACTTATTG TCCCAGATGT CATCAACACC CATCGACTGC 
CTGTTTAAGC ACATCGCTTC TGGCAACCAA AAGGAAGTAG AAAGACTTCT GAGTCAAGAT 
GACCAAGACA AAGACGCCAT GCAAAAGATG TGCCACCCGC TTTGCTCCTG TGAGGACTGT 
GAGAAGCTTG TCTCTGGAAG GCTGAATGAT CCGTCGGTTG TCACTCCTTT CTCCAGAGAT 
GACAGAGGTC AAACACCCCT CCATGTGGCT GCTCTCTGTG GTCAGGCATC CCTCATTGAC 
TTCCTGGTCT CCAAAGGTGC CGTAGTGAAC GCCACAGACT ATCATGGGTC TACTCCCTTA 
CATCTGGCAT GTCAGAAAGG CTTCCAAAGT GTGACGCTGC TGCTGCTACA CTACAAGGCC 
AACACTGAGG TGCAAGACAA CAACGGCAAC ACCCCGCTGC ACCTGGCCTG CACCTACGGG 
CAGGAGGATT GTGTGAAGGC CCTGGTCTAC TATGATGTCC AGGCCTGCAG ATTGGATATT 
GGTAACGAGA AAGGAGACAC AGCCCTGCAC ATTGCAGCTC GCTGGGGTTA CGAGGGCATC 
ATAGAGACGC TGCTGCAGAA TGGAGCCCCA ACAGCAATTC AGAACAGACT GAAGGAAACA 
CCACTCAAGT GTGCACTGAA CTCGAAGATT CTGTCTGCCA TGGAAGCGTA TCATCTATCC 
TCTGACAGGA GGCCAAAGCC TTCTGAGGTC CCTGCACCGT CCCCTACACG CTCTGCGGAT 
TCCATCAGCC AGGGCTCCTC TACCTCCAGC TTTTCTTCGG TATCGGTGAG CTTCAGGCCA 
GAAGAGGTCA AAAAGGACTA CAGGGAGGTA GAAAAGCTTC TCAGAGCAGT TGCCGATGGC 
GACCTAGAAA TGGTGCGATA CCTTTTGGAG TGGACGGAGG ACGACCTAGA CGATATGGAC 
GAAGCCATCA GCACGGTGGA TCTTGAATTC TGTCACCCTT TGTGCCAGTG CCCCAAGTGT 
GCTCCAGCCC AGAAGAAACT GGCAAGGATC CCTGCCAATG GGCTCAGTGT GAATGTGACC 
AACCAGGACG GCTTCTCTCC CCTGCATATG GCTGCCCTGC ATGGCAGGAC AGACCTTGTC 
CCCCTCCTGC TGAAGCATGG AGCCTACTCA GGTGCCAGAA ACACAAGCCA AGCTGTCCCG 
CTCCACCTGG CCTGCCAACA GGGCCACTTT CAGGTGGCAA AATGTCTCTT AGACTCCAAT 
GCAAAACCTA ATAAAAGGGA CCTCAGTGGG AACACGCCCC TCATTTGCGC CTGCTCTGCC 
GGCCATCATG AAGTCGCTGC ACTGTTGCTA CAGCACGGGG CCGCCATCAA TGCTTGCAAC 
AACAAAGGCA ACACGGCCCT GCACGAGGCC GTGATGGGAA GGCACGTCTT GGTGGTGGAA 
CTGCTTCTGT TTTATGGCGC ATCTGTTGAC ATCCTGAACA AGAGGCAGTA CACGGCGGTG 
GACTGTGCAG AGCAGGACTC AAAGATAATG GAGTTGCTGC AAGTCGTCCC AAGTTGCGTT 
GCTGCTTCTC CAAACAACGT CGTTGTCGTC CAGGAGACAG AACACGAGGA TTATGTGACT 
GTGGAGATCA GGAAAAAATG TAAGTGCTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd27 ( XM_017589355.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444844.1 Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56685
Clone ID Related Accession (Same CDS sequence)	XM_017589356.1 , XM_017589355.1
Accession Version	XM_017589355.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2793bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin repeat domain-containing protein 27 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCTGT ATGATGAAGA TCTCCTGAGA AACCCTTTCT ACCTGGCCCT TCAGAAGTGG 
CGCCCCGACT TGTGCAGTAA GGTGGCCCAG ATCCATGGCA TTGTCCTAGT GCCTTGCAGA 
GGAAGCTTGC CGAGCAACGT GCAGGCCTCC TGCCAGTTCG AGTCCTATGT TTTGGTACCC 
ATGGAAGGAC ACTTTCAGAC CTTAGATGGA AAGGCTGTCC TCATAGAAGG AAACAGGGTT 
AAGCTAGGAG CAGGCTTTGC TTGCCTTCTC TCTGTGCCCA TCCTCTTTGA AGAGACTTTC 
TACAACGAAA AGGAAGAGAG TTTCAGCATT CTCTGCATCG CTCATCCTTT GGAGAAGAGG 
GAGACATCAG AAGAACCTTC AGCACCCTCA GATCCTTTCT CTCTGAAAAC CATCAAAGAT 
GTGAGAGAAT TTTTGGGAAG ACACTCAGAG AGATTCGACA AGCACATCGC GTCTTTCCAC 
CGGACATTCC GAGAATGTGA AAGAAAGAGC CTCCGCCATC TCATAGACTC CGTAAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTC AGAGACTCTC ACCTGAAGGT TCTTGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTAGAGA TGTATGTCCA TCATGATATT 
TACGACCTGA TCTTCAAATA CGTGGGGACC ATGGAGGCGA GCGAGGATGC CGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTCCAG CAGAAAGACA TTGGCGTGAA ACCTGAGTTC 
AGCTTCAACA TTCCTCGGGC AAAGAGAGAG CTGGGACAGC TGAACAAGTG TACATCCCCA 
CAGCAGAAGC TGCTGTGTCT GCGGAAGGTG GTCCAGCTCA TGACACAGTC TCCCAGCCAG 
AGAGTGAATT TGGAGACCGT CTGTGCTGAT GATCTTCTTT CCGTCCTGTT ATATCTGCTT 
GTGAAAACAG AGATCCCTAA CTGGATGGCG AATTTGAGTT ACATCAAAAA CTTCAGATTT 
AGCAGCTCGC CCAAAGATGA GCTAGGATAC TGCCTGACCT CAGTCGAGGC TGCGATTGAA 
TACATCCGGC AAGGAAGCCT CTCTGCACAG CCGCCTGACG CTGAGGGATT TGGCGACAGA 
CTCTTTCTCA AGCAACGGAT GAACTTATTG TCCCAGATGT CATCAACACC CATCGACTGC 
CTGTTTAAGC ACATCGCTTC TGGCAACCAA AAGGAAGTAG AAAGACTTCT GAGTCAAGAT 
GACCAAGACA AAGACGCCAT GCAAAAGATG TGCCACCCGC TTTGCTCCTG TGAGGACTGT 
GAGAAGCTTG TCTCTGGAAG GCTGAATGAT CCGTCGGTTG TCACTCCTTT CTCCAGAGAT 
GACAGAGGTC AAACACCCCT CCATGTGGCT GCTCTCTGTG GTCAGGCATC CCTCATTGAC 
TTCCTGGTCT CCAAAGGTGC CGTAGTGAAC GCCACAGACT ATCATGGGTC TACTCCCTTA 
CATCTGGCAT GTCAGAAAGG CTTCCAAAGT GTGACGCTGC TGCTGCTACA CTACAAGGCC 
AACACTGAGG TGCAAGACAA CAACGGCAAC ACCCCGCTGC ACCTGGCCTG CACCTACGGG 
CAGGAGGATT GTGTGAAGGC CCTGGTCTAC TATGATGTCC AGGCCTGCAG ATTGGATATT 
GGTAACGAGA AAGGAGACAC AGCCCTGCAC ATTGCAGCTC GCTGGGGTTA CGAGGGCATC 
ATAGAGACGC TGCTGCAGAA TGGAGCCCCA ACAGCAATTC AGAACAGACT GAAGGAAACA 
CCACTCAAGT GTGCACTGAA CTCGAAGATT CTGTCTGCCA TGGAAGCGTA TCATCTATCC 
TCTGACAGGA GGCCAAAGCC TTCTGAGGTC CCTGCACCGT CCCCTACACG CTCTGCGGAT 
TCCATCAGCC AGGGCTCCTC TACCTCCAGC TTTTCTTCGG TATCGGTGAG CTTCAGGCCA 
GAAGAGGTCA AAAAGGACTA CAGGGAGGTA GAAAAGCTTC TCAGAGCAGT TGCCGATGGC 
GACCTAGAAA TGGTGCGATA CCTTTTGGAG TGGACGGAGG ACGACCTAGA CGATATGGAC 
GAAGCCATCA GCACGGTGGA TCTTGAATTC TGTCACCCTT TGTGCCAGTG CCCCAAGTGT 
GCTCCAGCCC AGAAGAAACT GGCAAGGATC CCTGCCAATG GGCTCAGTGT GAATGTGACC 
AACCAGGACG GCTTCTCTCC CCTGCATATG GCTGCCCTGC ATGGCAGGAC AGACCTTGTC 
CCCCTCCTGC TGAAGCATGG AGCCTACTCA GGTGCCAGAA ACACAAGCCA AGCTGTCCCG 
CTCCACCTGG CCTGCCAACA GGGCCACTTT CAGGTGGCAA AATGTCTCTT AGACTCCAAT 
GCAAAACCTA ATAAAAGGGA CCTCAGTGGG AACACGCCCC TCATTTGCGC CTGCTCTGCC 
GGCCATCATG AAGTCGCTGC ACTGTTGCTA CAGCACGGGG CCGCCATCAA TGCTTGCAAC 
AACAAAGGCA ACACGGCCCT GCACGAGGCC GTGATGGGAA GGCACGTCTT GGTGGTGGAA 
CTGCTTCTGT TTTATGGCGC ATCTGTTGAC ATCCTGAACA AGAGGCAGTA CACGGCGGTG 
GACTGTGCAG AGCAGGACTC AAAGATAATG GAGTTGCTGC AAGTCGTCCC AAGTTGCGTT 
GCTGCTTCTC CAAACAACGT CGTTGTCGTC CAGGAGACAG AACACGAGGA TTATGTGACT 
GTGGAGATCA GGAAAAAATG TAAGTGCTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444844.1
CDS	134..2926
Translation	MALYDEDLLRNPFYLALQKWRPDLCSKVAQIHGIVLVPCRGSLPSNVQASCQFESYVLVPMEGHFQTLDGKAVLIEGNRVKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLEKRETSEEPSAPSDPFSLKTIKDVREFLGRHSERFDKHIASFHRTFRECERKSLRHLIDSVNALYTKCLQQLLRDSHLKVLAKQEAQMNLMKQAVEMYVHHDIYDLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELGQLNKCTSPQQKLLCLRKVVQLMTQSPSQRVNLETVCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSSPKDELGYCLTSVEAAIEYIRQGSLSAQPPDAEGFGDRLFLKQRMNLLSQMSSTPIDCLFKHIASGNQKEVERLLSQDDQDKDAMQKMCHPLCSCEDCEKLVSGRLNDPSVVTPFSRDDRGQTPLHVAALCGQASLIDFLVSKGAVVNATDYHGSTPLHLACQKGFQSVTLLLLHYKANTEVQDNNGNTPLHLACTYGQEDCVKALVYYDVQACRLDIGNEKGDTALHIAARWGYEGIIETLLQNGAPTAIQNRLKETPLKCALNSKILSAMEAYHLSSDRRPKPSEVPAPSPTRSADSISQGSSTSSFSSVSVSFRPEEVKKDYREVEKLLRAVADGDLEMVRYLLEWTEDDLDDMDEAISTVDLEFCHPLCQCPKCAPAQKKLARIPANGLSVNVTNQDGFSPLHMAALHGRTDLVPLLLKHGAYSGARNTSQAVPLHLACQQGHFQVAKCLLDSNAKPNKRDLSGNTPLICACSAGHHEVAALLLQHGAAINACNNKGNTALHEAVMGRHVLVVELLLFYGASVDILNKRQYTAVDCAEQDSKIMELLQVVPSCVAASPNNVVVVQETEHEDYVTVEIRKKCKCF

Target ORF information:

RefSeq Version	XM_017589355.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589355.1

ORF Insert Sequence:

ATGGCTCTGT ATGATGAAGA TCTCCTGAGA AACCCTTTCT ACCTGGCCCT TCAGAAGTGG 
CGCCCCGACT TGTGCAGTAA GGTGGCCCAG ATCCATGGCA TTGTCCTAGT GCCTTGCAGA 
GGAAGCTTGC CGAGCAACGT GCAGGCCTCC TGCCAGTTCG AGTCCTATGT TTTGGTACCC 
ATGGAAGGAC ACTTTCAGAC CTTAGATGGA AAGGCTGTCC TCATAGAAGG AAACAGGGTT 
AAGCTAGGAG CAGGCTTTGC TTGCCTTCTC TCTGTGCCCA TCCTCTTTGA AGAGACTTTC 
TACAACGAAA AGGAAGAGAG TTTCAGCATT CTCTGCATCG CTCATCCTTT GGAGAAGAGG 
GAGACATCAG AAGAACCTTC AGCACCCTCA GATCCTTTCT CTCTGAAAAC CATCAAAGAT 
GTGAGAGAAT TTTTGGGAAG ACACTCAGAG AGATTCGACA AGCACATCGC GTCTTTCCAC 
CGGACATTCC GAGAATGTGA AAGAAAGAGC CTCCGCCATC TCATAGACTC CGTAAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTC AGAGACTCTC ACCTGAAGGT TCTTGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTAGAGA TGTATGTCCA TCATGATATT 
TACGACCTGA TCTTCAAATA CGTGGGGACC ATGGAGGCGA GCGAGGATGC CGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTCCAG CAGAAAGACA TTGGCGTGAA ACCTGAGTTC 
AGCTTCAACA TTCCTCGGGC AAAGAGAGAG CTGGGACAGC TGAACAAGTG TACATCCCCA 
CAGCAGAAGC TGCTGTGTCT GCGGAAGGTG GTCCAGCTCA TGACACAGTC TCCCAGCCAG 
AGAGTGAATT TGGAGACCGT CTGTGCTGAT GATCTTCTTT CCGTCCTGTT ATATCTGCTT 
GTGAAAACAG AGATCCCTAA CTGGATGGCG AATTTGAGTT ACATCAAAAA CTTCAGATTT 
AGCAGCTCGC CCAAAGATGA GCTAGGATAC TGCCTGACCT CAGTCGAGGC TGCGATTGAA 
TACATCCGGC AAGGAAGCCT CTCTGCACAG CCGCCTGACG CTGAGGGATT TGGCGACAGA 
CTCTTTCTCA AGCAACGGAT GAACTTATTG TCCCAGATGT CATCAACACC CATCGACTGC 
CTGTTTAAGC ACATCGCTTC TGGCAACCAA AAGGAAGTAG AAAGACTTCT GAGTCAAGAT 
GACCAAGACA AAGACGCCAT GCAAAAGATG TGCCACCCGC TTTGCTCCTG TGAGGACTGT 
GAGAAGCTTG TCTCTGGAAG GCTGAATGAT CCGTCGGTTG TCACTCCTTT CTCCAGAGAT 
GACAGAGGTC AAACACCCCT CCATGTGGCT GCTCTCTGTG GTCAGGCATC CCTCATTGAC 
TTCCTGGTCT CCAAAGGTGC CGTAGTGAAC GCCACAGACT ATCATGGGTC TACTCCCTTA 
CATCTGGCAT GTCAGAAAGG CTTCCAAAGT GTGACGCTGC TGCTGCTACA CTACAAGGCC 
AACACTGAGG TGCAAGACAA CAACGGCAAC ACCCCGCTGC ACCTGGCCTG CACCTACGGG 
CAGGAGGATT GTGTGAAGGC CCTGGTCTAC TATGATGTCC AGGCCTGCAG ATTGGATATT 
GGTAACGAGA AAGGAGACAC AGCCCTGCAC ATTGCAGCTC GCTGGGGTTA CGAGGGCATC 
ATAGAGACGC TGCTGCAGAA TGGAGCCCCA ACAGCAATTC AGAACAGACT GAAGGAAACA 
CCACTCAAGT GTGCACTGAA CTCGAAGATT CTGTCTGCCA TGGAAGCGTA TCATCTATCC 
TCTGACAGGA GGCCAAAGCC TTCTGAGGTC CCTGCACCGT CCCCTACACG CTCTGCGGAT 
TCCATCAGCC AGGGCTCCTC TACCTCCAGC TTTTCTTCGG TATCGGTGAG CTTCAGGCCA 
GAAGAGGTCA AAAAGGACTA CAGGGAGGTA GAAAAGCTTC TCAGAGCAGT TGCCGATGGC 
GACCTAGAAA TGGTGCGATA CCTTTTGGAG TGGACGGAGG ACGACCTAGA CGATATGGAC 
GAAGCCATCA GCACGGTGGA TCTTGAATTC TGTCACCCTT TGTGCCAGTG CCCCAAGTGT 
GCTCCAGCCC AGAAGAAACT GGCAAGGATC CCTGCCAATG GGCTCAGTGT GAATGTGACC 
AACCAGGACG GCTTCTCTCC CCTGCATATG GCTGCCCTGC ATGGCAGGAC AGACCTTGTC 
CCCCTCCTGC TGAAGCATGG AGCCTACTCA GGTGCCAGAA ACACAAGCCA AGCTGTCCCG 
CTCCACCTGG CCTGCCAACA GGGCCACTTT CAGGTGGCAA AATGTCTCTT AGACTCCAAT 
GCAAAACCTA ATAAAAGGGA CCTCAGTGGG AACACGCCCC TCATTTGCGC CTGCTCTGCC 
GGCCATCATG AAGTCGCTGC ACTGTTGCTA CAGCACGGGG CCGCCATCAA TGCTTGCAAC 
AACAAAGGCA ACACGGCCCT GCACGAGGCC GTGATGGGAA GGCACGTCTT GGTGGTGGAA 
CTGCTTCTGT TTTATGGCGC ATCTGTTGAC ATCCTGAACA AGAGGCAGTA CACGGCGGTG 
GACTGTGCAG AGCAGGACTC AAAGATAATG GAGTTGCTGC AAGTCGTCCC AAGTTGCGTT 
GCTGCTTCTC CAAACAACGT CGTTGTCGTC CAGGAGACAG AACACGAGGA TTATGTGACT 
GTGGAGATCA GGAAAAAATG TAAGTGCTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd27 ( XM_017589357.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444846.1 Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa56686
Clone ID Related Accession (Same CDS sequence)	XM_017589357.1
Accession Version	XM_017589357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin repeat domain-containing protein 27 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAGCTG CTTTGAGAAA GTTGTCCAGG TTTCCAGTGA GACCTGGAGG ATGGGTCCAT 
TTGCTTGTCA GAAATATTAA AAGAAAGCAG GTCCTAGTGC CTTGCAGAGG AAGCTTGCCG 
AGCAACGTGC AGGCCTCCTG CCAGTTCGAG TCCTATGTTT TGGTACCCAT GGAAGGACAC 
TTTCAGACCT TAGATGGAAA GGCTGTCCTC ATAGAAGGAA ACAGGGTTAA GCTAGGAGCA 
GGCTTTGCTT GCCTTCTCTC TGTGCCCATC CTCTTTGAAG AGACTTTCTA CAACGAAAAG 
GAAGAGAGTT TCAGCATTCT CTGCATCGCT CATCCTTTGG AGAAGAGGGA GACATCAGAA 
GAACCTTCAG CACCCTCAGA TCCTTTCTCT CTGAAAACCA TCAAAGATGT GAGAGAATTT 
TTGGGAAGAC ACTCAGAGAG ATTCGACAAG CACATCGCGT CTTTCCACCG GACATTCCGA 
GAATGTGAAA GAAAGAGCCT CCGCCATCTC ATAGACTCCG TAAATGCTCT CTACACCAAA 
TGCCTCCAGC AGCTTCTCAG AGACTCTCAC CTGAAGGTTC TTGCCAAGCA GGAGGCCCAG 
ATGAACCTGA TGAAGCAGGC AGTAGAGATG TATGTCCATC ATGATATTTA CGACCTGATC 
TTCAAATACG TGGGGACCAT GGAGGCGAGC GAGGATGCCG CCTTTAACAA AATCACAAGA 
AGCCTTCAAG ATCTCCAGCA GAAAGACATT GGCGTGAAAC CTGAGTTCAG CTTCAACATT 
CCTCGGGCAA AGAGAGAGCT GGGACAGCTG AACAAGTGTA CATCCCCACA GCAGAAGCTG 
CTGTGTCTGC GGAAGGTGGT CCAGCTCATG ACACAGTCTC CCAGCCAGAG AGTGAATTTG 
GAGACCGTCT GTGCTGATGA TCTTCTTTCC GTCCTGTTAT ATCTGCTTGT GAAAACAGAG 
ATCCCTAACT GGATGGCGAA TTTGAGTTAC ATCAAAAACT TCAGATTTAG CAGCTCGCCC 
AAAGATGAGC TAGGATACTG CCTGACCTCA GTCGAGGCTG CGATTGAATA CATCCGGCAA 
GGAAGCCTCT CTGCACAGCC GCCTGACGCT GAGGGATTTG GCGACAGACT CTTTCTCAAG 
CAACGGATGA ACTTATTGTC CCAGATGTCA TCAACACCCA TCGACTGCCT GTTTAAGCAC 
ATCGCTTCTG GCAACCAAAA GGAAGTAGAA AGACTTCTGA GTCAAGATGA CCAAGACAAA 
GACGCCATGC AAAAGATGTG CCACCCGCTT TGCTCCTGTG AGGACTGTGA GAAGCTTGTC 
TCTGGAAGGC TGAATGATCC GTCGGTTGTC ACTCCTTTCT CCAGAGATGA CAGAGGTCAA 
ACACCCCTCC ATGTGGCTGC TCTCTGTGGT CAGGCATCCC TCATTGACTT CCTGGTCTCC 
AAAGGTGCCG TAGTGAACGC CACAGACTAT CATGGGTCTA CTCCCTTACA TCTGGCATGT 
CAGAAAGGCT TCCAAAGTGT GACGCTGCTG CTGCTACACT ACAAGGCCAA CACTGAGGTG 
CAAGACAACA ACGGCAACAC CCCGCTGCAC CTGGCCTGCA CCTACGGGCA GGAGGATTGT 
GTGAAGGCCC TGGTCTACTA TGATGTCCAG GCCTGCAGAT TGGATATTGG TAACGAGAAA 
GGAGACACAG CCCTGCACAT TGCAGCTCGC TGGGGTTACG AGGGCATCAT AGAGACGCTG 
CTGCAGAATG GAGCCCCAAC AGCAATTCAG AACAGACTGA AGGAAACACC ACTCAAGTGT 
GCACTGAACT CGAAGATTCT GTCTGCCATG GAAGCGTATC ATCTATCCTC TGACAGGAGG 
CCAAAGCCTT CTGAGGTCCC TGCACCGTCC CCTACACGCT CTGCGGATTC CATCAGCCAG 
GGCTCCTCTA CCTCCAGCTT TTCTTCGGTA TCGGTGAGCT TCAGGCCAGA AGAGGTCAAA 
AAGGACTACA GGGAGGTAGA AAAGCTTCTC AGAGCAGTTG CCGATGGCGA CCTAGAAATG 
GTGCGATACC TTTTGGAGTG GACGGAGGAC GACCTAGACG ATATGGACGA AGCCATCAGC 
ACGGTGGATC TTGAATTCTG TCACCCTTTG TGCCAGTGCC CCAAGTGTGC TCCAGCCCAG 
AAGAAACTGG CAAGGATCCC TGCCAATGGG CTCAGTGTGA ATGTGACCAA CCAGGACGGC 
TTCTCTCCCC TGCATATGGC TGCCCTGCAT GGCAGGACAG ACCTTGTCCC CCTCCTGCTG 
AAGCATGGAG CCTACTCAGG TGCCAGAAAC ACAAGCCAAG CTGTCCCGCT CCACCTGGCC 
TGCCAACAGG GCCACTTTCA GGTGGCAAAA TGTCTCTTAG ACTCCAATGC AAAACCTAAT 
AAAAGGGACC TCAGTGGGAA CACGCCCCTC ATTTGCGCCT GCTCTGCCGG CCATCATGAA 
GTCGCTGCAC TGTTGCTACA GCACGGGGCC GCCATCAATG CTTGCAACAA CAAAGGCAAC 
ACGGCCCTGC ACGAGGCCGT GATGGGAAGG CACGTCTTGG TGGTGGAACT GCTTCTGTTT 
TATGGCGCAT CTGTTGACAT CCTGAACAAG AGGCAGTACA CGGCGGTGGA CTGTGCAGAG 
CAGGACTCAA AGATAATGGA GTTGCTGCAA GTCGTCCCAA GTTGCGTTGC TGCTTCTCCA 
AACAACGTCG TTGTCGTCCA GGAGACAGAA CACGAGGATT ATGTGACTGT GGAGATCAGG 
AAAAAATGTA AGTGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444846.1
CDS	160..2940
Translation	MTAALRKLSRFPVRPGGWVHLLVRNIKRKQVLVPCRGSLPSNVQASCQFESYVLVPMEGHFQTLDGKAVLIEGNRVKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLEKRETSEEPSAPSDPFSLKTIKDVREFLGRHSERFDKHIASFHRTFRECERKSLRHLIDSVNALYTKCLQQLLRDSHLKVLAKQEAQMNLMKQAVEMYVHHDIYDLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELGQLNKCTSPQQKLLCLRKVVQLMTQSPSQRVNLETVCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSSPKDELGYCLTSVEAAIEYIRQGSLSAQPPDAEGFGDRLFLKQRMNLLSQMSSTPIDCLFKHIASGNQKEVERLLSQDDQDKDAMQKMCHPLCSCEDCEKLVSGRLNDPSVVTPFSRDDRGQTPLHVAALCGQASLIDFLVSKGAVVNATDYHGSTPLHLACQKGFQSVTLLLLHYKANTEVQDNNGNTPLHLACTYGQEDCVKALVYYDVQACRLDIGNEKGDTALHIAARWGYEGIIETLLQNGAPTAIQNRLKETPLKCALNSKILSAMEAYHLSSDRRPKPSEVPAPSPTRSADSISQGSSTSSFSSVSVSFRPEEVKKDYREVEKLLRAVADGDLEMVRYLLEWTEDDLDDMDEAISTVDLEFCHPLCQCPKCAPAQKKLARIPANGLSVNVTNQDGFSPLHMAALHGRTDLVPLLLKHGAYSGARNTSQAVPLHLACQQGHFQVAKCLLDSNAKPNKRDLSGNTPLICACSAGHHEVAALLLQHGAAINACNNKGNTALHEAVMGRHVLVVELLLFYGASVDILNKRQYTAVDCAEQDSKIMELLQVVPSCVAASPNNVVVVQETEHEDYVTVEIRKKCKCF

Target ORF information:

RefSeq Version	XM_017589357.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589357.1

ORF Insert Sequence:

ATGACAGCTG CTTTGAGAAA GTTGTCCAGG TTTCCAGTGA GACCTGGAGG ATGGGTCCAT 
TTGCTTGTCA GAAATATTAA AAGAAAGCAG GTCCTAGTGC CTTGCAGAGG AAGCTTGCCG 
AGCAACGTGC AGGCCTCCTG CCAGTTCGAG TCCTATGTTT TGGTACCCAT GGAAGGACAC 
TTTCAGACCT TAGATGGAAA GGCTGTCCTC ATAGAAGGAA ACAGGGTTAA GCTAGGAGCA 
GGCTTTGCTT GCCTTCTCTC TGTGCCCATC CTCTTTGAAG AGACTTTCTA CAACGAAAAG 
GAAGAGAGTT TCAGCATTCT CTGCATCGCT CATCCTTTGG AGAAGAGGGA GACATCAGAA 
GAACCTTCAG CACCCTCAGA TCCTTTCTCT CTGAAAACCA TCAAAGATGT GAGAGAATTT 
TTGGGAAGAC ACTCAGAGAG ATTCGACAAG CACATCGCGT CTTTCCACCG GACATTCCGA 
GAATGTGAAA GAAAGAGCCT CCGCCATCTC ATAGACTCCG TAAATGCTCT CTACACCAAA 
TGCCTCCAGC AGCTTCTCAG AGACTCTCAC CTGAAGGTTC TTGCCAAGCA GGAGGCCCAG 
ATGAACCTGA TGAAGCAGGC AGTAGAGATG TATGTCCATC ATGATATTTA CGACCTGATC 
TTCAAATACG TGGGGACCAT GGAGGCGAGC GAGGATGCCG CCTTTAACAA AATCACAAGA 
AGCCTTCAAG ATCTCCAGCA GAAAGACATT GGCGTGAAAC CTGAGTTCAG CTTCAACATT 
CCTCGGGCAA AGAGAGAGCT GGGACAGCTG AACAAGTGTA CATCCCCACA GCAGAAGCTG 
CTGTGTCTGC GGAAGGTGGT CCAGCTCATG ACACAGTCTC CCAGCCAGAG AGTGAATTTG 
GAGACCGTCT GTGCTGATGA TCTTCTTTCC GTCCTGTTAT ATCTGCTTGT GAAAACAGAG 
ATCCCTAACT GGATGGCGAA TTTGAGTTAC ATCAAAAACT TCAGATTTAG CAGCTCGCCC 
AAAGATGAGC TAGGATACTG CCTGACCTCA GTCGAGGCTG CGATTGAATA CATCCGGCAA 
GGAAGCCTCT CTGCACAGCC GCCTGACGCT GAGGGATTTG GCGACAGACT CTTTCTCAAG 
CAACGGATGA ACTTATTGTC CCAGATGTCA TCAACACCCA TCGACTGCCT GTTTAAGCAC 
ATCGCTTCTG GCAACCAAAA GGAAGTAGAA AGACTTCTGA GTCAAGATGA CCAAGACAAA 
GACGCCATGC AAAAGATGTG CCACCCGCTT TGCTCCTGTG AGGACTGTGA GAAGCTTGTC 
TCTGGAAGGC TGAATGATCC GTCGGTTGTC ACTCCTTTCT CCAGAGATGA CAGAGGTCAA 
ACACCCCTCC ATGTGGCTGC TCTCTGTGGT CAGGCATCCC TCATTGACTT CCTGGTCTCC 
AAAGGTGCCG TAGTGAACGC CACAGACTAT CATGGGTCTA CTCCCTTACA TCTGGCATGT 
CAGAAAGGCT TCCAAAGTGT GACGCTGCTG CTGCTACACT ACAAGGCCAA CACTGAGGTG 
CAAGACAACA ACGGCAACAC CCCGCTGCAC CTGGCCTGCA CCTACGGGCA GGAGGATTGT 
GTGAAGGCCC TGGTCTACTA TGATGTCCAG GCCTGCAGAT TGGATATTGG TAACGAGAAA 
GGAGACACAG CCCTGCACAT TGCAGCTCGC TGGGGTTACG AGGGCATCAT AGAGACGCTG 
CTGCAGAATG GAGCCCCAAC AGCAATTCAG AACAGACTGA AGGAAACACC ACTCAAGTGT 
GCACTGAACT CGAAGATTCT GTCTGCCATG GAAGCGTATC ATCTATCCTC TGACAGGAGG 
CCAAAGCCTT CTGAGGTCCC TGCACCGTCC CCTACACGCT CTGCGGATTC CATCAGCCAG 
GGCTCCTCTA CCTCCAGCTT TTCTTCGGTA TCGGTGAGCT TCAGGCCAGA AGAGGTCAAA 
AAGGACTACA GGGAGGTAGA AAAGCTTCTC AGAGCAGTTG CCGATGGCGA CCTAGAAATG 
GTGCGATACC TTTTGGAGTG GACGGAGGAC GACCTAGACG ATATGGACGA AGCCATCAGC 
ACGGTGGATC TTGAATTCTG TCACCCTTTG TGCCAGTGCC CCAAGTGTGC TCCAGCCCAG 
AAGAAACTGG CAAGGATCCC TGCCAATGGG CTCAGTGTGA ATGTGACCAA CCAGGACGGC 
TTCTCTCCCC TGCATATGGC TGCCCTGCAT GGCAGGACAG ACCTTGTCCC CCTCCTGCTG 
AAGCATGGAG CCTACTCAGG TGCCAGAAAC ACAAGCCAAG CTGTCCCGCT CCACCTGGCC 
TGCCAACAGG GCCACTTTCA GGTGGCAAAA TGTCTCTTAG ACTCCAATGC AAAACCTAAT 
AAAAGGGACC TCAGTGGGAA CACGCCCCTC ATTTGCGCCT GCTCTGCCGG CCATCATGAA 
GTCGCTGCAC TGTTGCTACA GCACGGGGCC GCCATCAATG CTTGCAACAA CAAAGGCAAC 
ACGGCCCTGC ACGAGGCCGT GATGGGAAGG CACGTCTTGG TGGTGGAACT GCTTCTGTTT 
TATGGCGCAT CTGTTGACAT CCTGAACAAG AGGCAGTACA CGGCGGTGGA CTGTGCAGAG 
CAGGACTCAA AGATAATGGA GTTGCTGCAA GTCGTCCCAA GTTGCGTTGC TGCTTCTCCA 
AACAACGTCG TTGTCGTCCA GGAGACAGAA CACGAGGATT ATGTGACTGT GGAGATCAGG 
AAAAAATGTA AGTGCTTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd27 ( XM_017589358.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444847.1 Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa56687
Clone ID Related Accession (Same CDS sequence)	XM_017589358.1 , XM_017589359.1
Accession Version	XM_017589358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin repeat domain-containing protein 27 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGAC ACTTTCAGAC CTTAGATGGA AAGGCTGTCC TCATAGAAGG AAACAGGGTT 
AAGCTAGGAG CAGGCTTTGC TTGCCTTCTC TCTGTGCCCA TCCTCTTTGA AGAGACTTTC 
TACAACGAAA AGGAAGAGAG TTTCAGCATT CTCTGCATCG CTCATCCTTT GGAGAAGAGG 
GAGACATCAG AAGAACCTTC AGCACCCTCA GATCCTTTCT CTCTGAAAAC CATCAAAGAT 
GTGAGAGAAT TTTTGGGAAG ACACTCAGAG AGATTCGACA AGCACATCGC GTCTTTCCAC 
CGGACATTCC GAGAATGTGA AAGAAAGAGC CTCCGCCATC TCATAGACTC CGTAAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTC AGAGACTCTC ACCTGAAGGT TCTTGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTAGAGA TGTATGTCCA TCATGATATT 
TACGACCTGA TCTTCAAATA CGTGGGGACC ATGGAGGCGA GCGAGGATGC CGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTCCAG CAGAAAGACA TTGGCGTGAA ACCTGAGTTC 
AGCTTCAACA TTCCTCGGGC AAAGAGAGAG CTGGGACAGC TGAACAAGTG TACATCCCCA 
CAGCAGAAGC TGCTGTGTCT GCGGAAGGTG GTCCAGCTCA TGACACAGTC TCCCAGCCAG 
AGAGTGAATT TGGAGACCGT CTGTGCTGAT GATCTTCTTT CCGTCCTGTT ATATCTGCTT 
GTGAAAACAG AGATCCCTAA CTGGATGGCG AATTTGAGTT ACATCAAAAA CTTCAGATTT 
AGCAGCTCGC CCAAAGATGA GCTAGGATAC TGCCTGACCT CAGTCGAGGC TGCGATTGAA 
TACATCCGGC AAGGAAGCCT CTCTGCACAG CCGCCTGACG CTGAGGGATT TGGCGACAGA 
CTCTTTCTCA AGCAACGGAT GAACTTATTG TCCCAGATGT CATCAACACC CATCGACTGC 
CTGTTTAAGC ACATCGCTTC TGGCAACCAA AAGGAAGTAG AAAGACTTCT GAGTCAAGAT 
GACCAAGACA AAGACGCCAT GCAAAAGATG TGCCACCCGC TTTGCTCCTG TGAGGACTGT 
GAGAAGCTTG TCTCTGGAAG GCTGAATGAT CCGTCGGTTG TCACTCCTTT CTCCAGAGAT 
GACAGAGGTC AAACACCCCT CCATGTGGCT GCTCTCTGTG GTCAGGCATC CCTCATTGAC 
TTCCTGGTCT CCAAAGGTGC CGTAGTGAAC GCCACAGACT ATCATGGGTC TACTCCCTTA 
CATCTGGCAT GTCAGAAAGG CTTCCAAAGT GTGACGCTGC TGCTGCTACA CTACAAGGCC 
AACACTGAGG TGCAAGACAA CAACGGCAAC ACCCCGCTGC ACCTGGCCTG CACCTACGGG 
CAGGAGGATT GTGTGAAGGC CCTGGTCTAC TATGATGTCC AGGCCTGCAG ATTGGATATT 
GGTAACGAGA AAGGAGACAC AGCCCTGCAC ATTGCAGCTC GCTGGGGTTA CGAGGGCATC 
ATAGAGACGC TGCTGCAGAA TGGAGCCCCA ACAGCAATTC AGAACAGACT GAAGGAAACA 
CCACTCAAGT GTGCACTGAA CTCGAAGATT CTGTCTGCCA TGGAAGCGTA TCATCTATCC 
TCTGACAGGA GGCCAAAGCC TTCTGAGGTC CCTGCACCGT CCCCTACACG CTCTGCGGAT 
TCCATCAGCC AGGGCTCCTC TACCTCCAGC TTTTCTTCGG TATCGGTGAG CTTCAGGCCA 
GAAGAGGTCA AAAAGGACTA CAGGGAGGTA GAAAAGCTTC TCAGAGCAGT TGCCGATGGC 
GACCTAGAAA TGGTGCGATA CCTTTTGGAG TGGACGGAGG ACGACCTAGA CGATATGGAC 
GAAGCCATCA GCACGGTGGA TCTTGAATTC TGTCACCCTT TGTGCCAGTG CCCCAAGTGT 
GCTCCAGCCC AGAAGAAACT GGCAAGGATC CCTGCCAATG GGCTCAGTGT GAATGTGACC 
AACCAGGACG GCTTCTCTCC CCTGCATATG GCTGCCCTGC ATGGCAGGAC AGACCTTGTC 
CCCCTCCTGC TGAAGCATGG AGCCTACTCA GGTGCCAGAA ACACAAGCCA AGCTGTCCCG 
CTCCACCTGG CCTGCCAACA GGGCCACTTT CAGGTGGCAA AATGTCTCTT AGACTCCAAT 
GCAAAACCTA ATAAAAGGGA CCTCAGTGGG AACACGCCCC TCATTTGCGC CTGCTCTGCC 
GGCCATCATG AAGTCGCTGC ACTGTTGCTA CAGCACGGGG CCGCCATCAA TGCTTGCAAC 
AACAAAGGCA ACACGGCCCT GCACGAGGCC GTGATGGGAA GGCACGTCTT GGTGGTGGAA 
CTGCTTCTGT TTTATGGCGC ATCTGTTGAC ATCCTGAACA AGAGGCAGTA CACGGCGGTG 
GACTGTGCAG AGCAGGACTC AAAGATAATG GAGTTGCTGC AAGTCGTCCC AAGTTGCGTT 
GCTGCTTCTC CAAACAACGT CGTTGTCGTC CAGGAGACAG AACACGAGGA TTATGTGACT 
GTGGAGATCA GGAAAAAATG TAAGTGCTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444847.1
CDS	298..2910
Translation	MEGHFQTLDGKAVLIEGNRVKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLEKRETSEEPSAPSDPFSLKTIKDVREFLGRHSERFDKHIASFHRTFRECERKSLRHLIDSVNALYTKCLQQLLRDSHLKVLAKQEAQMNLMKQAVEMYVHHDIYDLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELGQLNKCTSPQQKLLCLRKVVQLMTQSPSQRVNLETVCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSSPKDELGYCLTSVEAAIEYIRQGSLSAQPPDAEGFGDRLFLKQRMNLLSQMSSTPIDCLFKHIASGNQKEVERLLSQDDQDKDAMQKMCHPLCSCEDCEKLVSGRLNDPSVVTPFSRDDRGQTPLHVAALCGQASLIDFLVSKGAVVNATDYHGSTPLHLACQKGFQSVTLLLLHYKANTEVQDNNGNTPLHLACTYGQEDCVKALVYYDVQACRLDIGNEKGDTALHIAARWGYEGIIETLLQNGAPTAIQNRLKETPLKCALNSKILSAMEAYHLSSDRRPKPSEVPAPSPTRSADSISQGSSTSSFSSVSVSFRPEEVKKDYREVEKLLRAVADGDLEMVRYLLEWTEDDLDDMDEAISTVDLEFCHPLCQCPKCAPAQKKLARIPANGLSVNVTNQDGFSPLHMAALHGRTDLVPLLLKHGAYSGARNTSQAVPLHLACQQGHFQVAKCLLDSNAKPNKRDLSGNTPLICACSAGHHEVAALLLQHGAAINACNNKGNTALHEAVMGRHVLVVELLLFYGASVDILNKRQYTAVDCAEQDSKIMELLQVVPSCVAASPNNVVVVQETEHEDYVTVEIRKKCKCF

Target ORF information:

RefSeq Version	XM_017589358.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589358.1

ORF Insert Sequence:

ATGGAAGGAC ACTTTCAGAC CTTAGATGGA AAGGCTGTCC TCATAGAAGG AAACAGGGTT 
AAGCTAGGAG CAGGCTTTGC TTGCCTTCTC TCTGTGCCCA TCCTCTTTGA AGAGACTTTC 
TACAACGAAA AGGAAGAGAG TTTCAGCATT CTCTGCATCG CTCATCCTTT GGAGAAGAGG 
GAGACATCAG AAGAACCTTC AGCACCCTCA GATCCTTTCT CTCTGAAAAC CATCAAAGAT 
GTGAGAGAAT TTTTGGGAAG ACACTCAGAG AGATTCGACA AGCACATCGC GTCTTTCCAC 
CGGACATTCC GAGAATGTGA AAGAAAGAGC CTCCGCCATC TCATAGACTC CGTAAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTC AGAGACTCTC ACCTGAAGGT TCTTGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTAGAGA TGTATGTCCA TCATGATATT 
TACGACCTGA TCTTCAAATA CGTGGGGACC ATGGAGGCGA GCGAGGATGC CGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTCCAG CAGAAAGACA TTGGCGTGAA ACCTGAGTTC 
AGCTTCAACA TTCCTCGGGC AAAGAGAGAG CTGGGACAGC TGAACAAGTG TACATCCCCA 
CAGCAGAAGC TGCTGTGTCT GCGGAAGGTG GTCCAGCTCA TGACACAGTC TCCCAGCCAG 
AGAGTGAATT TGGAGACCGT CTGTGCTGAT GATCTTCTTT CCGTCCTGTT ATATCTGCTT 
GTGAAAACAG AGATCCCTAA CTGGATGGCG AATTTGAGTT ACATCAAAAA CTTCAGATTT 
AGCAGCTCGC CCAAAGATGA GCTAGGATAC TGCCTGACCT CAGTCGAGGC TGCGATTGAA 
TACATCCGGC AAGGAAGCCT CTCTGCACAG CCGCCTGACG CTGAGGGATT TGGCGACAGA 
CTCTTTCTCA AGCAACGGAT GAACTTATTG TCCCAGATGT CATCAACACC CATCGACTGC 
CTGTTTAAGC ACATCGCTTC TGGCAACCAA AAGGAAGTAG AAAGACTTCT GAGTCAAGAT 
GACCAAGACA AAGACGCCAT GCAAAAGATG TGCCACCCGC TTTGCTCCTG TGAGGACTGT 
GAGAAGCTTG TCTCTGGAAG GCTGAATGAT CCGTCGGTTG TCACTCCTTT CTCCAGAGAT 
GACAGAGGTC AAACACCCCT CCATGTGGCT GCTCTCTGTG GTCAGGCATC CCTCATTGAC 
TTCCTGGTCT CCAAAGGTGC CGTAGTGAAC GCCACAGACT ATCATGGGTC TACTCCCTTA 
CATCTGGCAT GTCAGAAAGG CTTCCAAAGT GTGACGCTGC TGCTGCTACA CTACAAGGCC 
AACACTGAGG TGCAAGACAA CAACGGCAAC ACCCCGCTGC ACCTGGCCTG CACCTACGGG 
CAGGAGGATT GTGTGAAGGC CCTGGTCTAC TATGATGTCC AGGCCTGCAG ATTGGATATT 
GGTAACGAGA AAGGAGACAC AGCCCTGCAC ATTGCAGCTC GCTGGGGTTA CGAGGGCATC 
ATAGAGACGC TGCTGCAGAA TGGAGCCCCA ACAGCAATTC AGAACAGACT GAAGGAAACA 
CCACTCAAGT GTGCACTGAA CTCGAAGATT CTGTCTGCCA TGGAAGCGTA TCATCTATCC 
TCTGACAGGA GGCCAAAGCC TTCTGAGGTC CCTGCACCGT CCCCTACACG CTCTGCGGAT 
TCCATCAGCC AGGGCTCCTC TACCTCCAGC TTTTCTTCGG TATCGGTGAG CTTCAGGCCA 
GAAGAGGTCA AAAAGGACTA CAGGGAGGTA GAAAAGCTTC TCAGAGCAGT TGCCGATGGC 
GACCTAGAAA TGGTGCGATA CCTTTTGGAG TGGACGGAGG ACGACCTAGA CGATATGGAC 
GAAGCCATCA GCACGGTGGA TCTTGAATTC TGTCACCCTT TGTGCCAGTG CCCCAAGTGT 
GCTCCAGCCC AGAAGAAACT GGCAAGGATC CCTGCCAATG GGCTCAGTGT GAATGTGACC 
AACCAGGACG GCTTCTCTCC CCTGCATATG GCTGCCCTGC ATGGCAGGAC AGACCTTGTC 
CCCCTCCTGC TGAAGCATGG AGCCTACTCA GGTGCCAGAA ACACAAGCCA AGCTGTCCCG 
CTCCACCTGG CCTGCCAACA GGGCCACTTT CAGGTGGCAA AATGTCTCTT AGACTCCAAT 
GCAAAACCTA ATAAAAGGGA CCTCAGTGGG AACACGCCCC TCATTTGCGC CTGCTCTGCC 
GGCCATCATG AAGTCGCTGC ACTGTTGCTA CAGCACGGGG CCGCCATCAA TGCTTGCAAC 
AACAAAGGCA ACACGGCCCT GCACGAGGCC GTGATGGGAA GGCACGTCTT GGTGGTGGAA 
CTGCTTCTGT TTTATGGCGC ATCTGTTGAC ATCCTGAACA AGAGGCAGTA CACGGCGGTG 
GACTGTGCAG AGCAGGACTC AAAGATAATG GAGTTGCTGC AAGTCGTCCC AAGTTGCGTT 
GCTGCTTCTC CAAACAACGT CGTTGTCGTC CAGGAGACAG AACACGAGGA TTATGTGACT 
GTGGAGATCA GGAAAAAATG TAAGTGCTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd27 ( XM_017589359.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444848.1 Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa56687
Clone ID Related Accession (Same CDS sequence)	XM_017589358.1 , XM_017589359.1
Accession Version	XM_017589359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2613bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin repeat domain-containing protein 27 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGGAC ACTTTCAGAC CTTAGATGGA AAGGCTGTCC TCATAGAAGG AAACAGGGTT 
AAGCTAGGAG CAGGCTTTGC TTGCCTTCTC TCTGTGCCCA TCCTCTTTGA AGAGACTTTC 
TACAACGAAA AGGAAGAGAG TTTCAGCATT CTCTGCATCG CTCATCCTTT GGAGAAGAGG 
GAGACATCAG AAGAACCTTC AGCACCCTCA GATCCTTTCT CTCTGAAAAC CATCAAAGAT 
GTGAGAGAAT TTTTGGGAAG ACACTCAGAG AGATTCGACA AGCACATCGC GTCTTTCCAC 
CGGACATTCC GAGAATGTGA AAGAAAGAGC CTCCGCCATC TCATAGACTC CGTAAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTC AGAGACTCTC ACCTGAAGGT TCTTGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTAGAGA TGTATGTCCA TCATGATATT 
TACGACCTGA TCTTCAAATA CGTGGGGACC ATGGAGGCGA GCGAGGATGC CGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTCCAG CAGAAAGACA TTGGCGTGAA ACCTGAGTTC 
AGCTTCAACA TTCCTCGGGC AAAGAGAGAG CTGGGACAGC TGAACAAGTG TACATCCCCA 
CAGCAGAAGC TGCTGTGTCT GCGGAAGGTG GTCCAGCTCA TGACACAGTC TCCCAGCCAG 
AGAGTGAATT TGGAGACCGT CTGTGCTGAT GATCTTCTTT CCGTCCTGTT ATATCTGCTT 
GTGAAAACAG AGATCCCTAA CTGGATGGCG AATTTGAGTT ACATCAAAAA CTTCAGATTT 
AGCAGCTCGC CCAAAGATGA GCTAGGATAC TGCCTGACCT CAGTCGAGGC TGCGATTGAA 
TACATCCGGC AAGGAAGCCT CTCTGCACAG CCGCCTGACG CTGAGGGATT TGGCGACAGA 
CTCTTTCTCA AGCAACGGAT GAACTTATTG TCCCAGATGT CATCAACACC CATCGACTGC 
CTGTTTAAGC ACATCGCTTC TGGCAACCAA AAGGAAGTAG AAAGACTTCT GAGTCAAGAT 
GACCAAGACA AAGACGCCAT GCAAAAGATG TGCCACCCGC TTTGCTCCTG TGAGGACTGT 
GAGAAGCTTG TCTCTGGAAG GCTGAATGAT CCGTCGGTTG TCACTCCTTT CTCCAGAGAT 
GACAGAGGTC AAACACCCCT CCATGTGGCT GCTCTCTGTG GTCAGGCATC CCTCATTGAC 
TTCCTGGTCT CCAAAGGTGC CGTAGTGAAC GCCACAGACT ATCATGGGTC TACTCCCTTA 
CATCTGGCAT GTCAGAAAGG CTTCCAAAGT GTGACGCTGC TGCTGCTACA CTACAAGGCC 
AACACTGAGG TGCAAGACAA CAACGGCAAC ACCCCGCTGC ACCTGGCCTG CACCTACGGG 
CAGGAGGATT GTGTGAAGGC CCTGGTCTAC TATGATGTCC AGGCCTGCAG ATTGGATATT 
GGTAACGAGA AAGGAGACAC AGCCCTGCAC ATTGCAGCTC GCTGGGGTTA CGAGGGCATC 
ATAGAGACGC TGCTGCAGAA TGGAGCCCCA ACAGCAATTC AGAACAGACT GAAGGAAACA 
CCACTCAAGT GTGCACTGAA CTCGAAGATT CTGTCTGCCA TGGAAGCGTA TCATCTATCC 
TCTGACAGGA GGCCAAAGCC TTCTGAGGTC CCTGCACCGT CCCCTACACG CTCTGCGGAT 
TCCATCAGCC AGGGCTCCTC TACCTCCAGC TTTTCTTCGG TATCGGTGAG CTTCAGGCCA 
GAAGAGGTCA AAAAGGACTA CAGGGAGGTA GAAAAGCTTC TCAGAGCAGT TGCCGATGGC 
GACCTAGAAA TGGTGCGATA CCTTTTGGAG TGGACGGAGG ACGACCTAGA CGATATGGAC 
GAAGCCATCA GCACGGTGGA TCTTGAATTC TGTCACCCTT TGTGCCAGTG CCCCAAGTGT 
GCTCCAGCCC AGAAGAAACT GGCAAGGATC CCTGCCAATG GGCTCAGTGT GAATGTGACC 
AACCAGGACG GCTTCTCTCC CCTGCATATG GCTGCCCTGC ATGGCAGGAC AGACCTTGTC 
CCCCTCCTGC TGAAGCATGG AGCCTACTCA GGTGCCAGAA ACACAAGCCA AGCTGTCCCG 
CTCCACCTGG CCTGCCAACA GGGCCACTTT CAGGTGGCAA AATGTCTCTT AGACTCCAAT 
GCAAAACCTA ATAAAAGGGA CCTCAGTGGG AACACGCCCC TCATTTGCGC CTGCTCTGCC 
GGCCATCATG AAGTCGCTGC ACTGTTGCTA CAGCACGGGG CCGCCATCAA TGCTTGCAAC 
AACAAAGGCA ACACGGCCCT GCACGAGGCC GTGATGGGAA GGCACGTCTT GGTGGTGGAA 
CTGCTTCTGT TTTATGGCGC ATCTGTTGAC ATCCTGAACA AGAGGCAGTA CACGGCGGTG 
GACTGTGCAG AGCAGGACTC AAAGATAATG GAGTTGCTGC AAGTCGTCCC AAGTTGCGTT 
GCTGCTTCTC CAAACAACGT CGTTGTCGTC CAGGAGACAG AACACGAGGA TTATGTGACT 
GTGGAGATCA GGAAAAAATG TAAGTGCTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444848.1
CDS	287..2899
Translation	MEGHFQTLDGKAVLIEGNRVKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLEKRETSEEPSAPSDPFSLKTIKDVREFLGRHSERFDKHIASFHRTFRECERKSLRHLIDSVNALYTKCLQQLLRDSHLKVLAKQEAQMNLMKQAVEMYVHHDIYDLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELGQLNKCTSPQQKLLCLRKVVQLMTQSPSQRVNLETVCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSSPKDELGYCLTSVEAAIEYIRQGSLSAQPPDAEGFGDRLFLKQRMNLLSQMSSTPIDCLFKHIASGNQKEVERLLSQDDQDKDAMQKMCHPLCSCEDCEKLVSGRLNDPSVVTPFSRDDRGQTPLHVAALCGQASLIDFLVSKGAVVNATDYHGSTPLHLACQKGFQSVTLLLLHYKANTEVQDNNGNTPLHLACTYGQEDCVKALVYYDVQACRLDIGNEKGDTALHIAARWGYEGIIETLLQNGAPTAIQNRLKETPLKCALNSKILSAMEAYHLSSDRRPKPSEVPAPSPTRSADSISQGSSTSSFSSVSVSFRPEEVKKDYREVEKLLRAVADGDLEMVRYLLEWTEDDLDDMDEAISTVDLEFCHPLCQCPKCAPAQKKLARIPANGLSVNVTNQDGFSPLHMAALHGRTDLVPLLLKHGAYSGARNTSQAVPLHLACQQGHFQVAKCLLDSNAKPNKRDLSGNTPLICACSAGHHEVAALLLQHGAAINACNNKGNTALHEAVMGRHVLVVELLLFYGASVDILNKRQYTAVDCAEQDSKIMELLQVVPSCVAASPNNVVVVQETEHEDYVTVEIRKKCKCF

Target ORF information:

RefSeq Version	XM_017589359.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589359.1

ORF Insert Sequence:

ATGGAAGGAC ACTTTCAGAC CTTAGATGGA AAGGCTGTCC TCATAGAAGG AAACAGGGTT 
AAGCTAGGAG CAGGCTTTGC TTGCCTTCTC TCTGTGCCCA TCCTCTTTGA AGAGACTTTC 
TACAACGAAA AGGAAGAGAG TTTCAGCATT CTCTGCATCG CTCATCCTTT GGAGAAGAGG 
GAGACATCAG AAGAACCTTC AGCACCCTCA GATCCTTTCT CTCTGAAAAC CATCAAAGAT 
GTGAGAGAAT TTTTGGGAAG ACACTCAGAG AGATTCGACA AGCACATCGC GTCTTTCCAC 
CGGACATTCC GAGAATGTGA AAGAAAGAGC CTCCGCCATC TCATAGACTC CGTAAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTC AGAGACTCTC ACCTGAAGGT TCTTGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTAGAGA TGTATGTCCA TCATGATATT 
TACGACCTGA TCTTCAAATA CGTGGGGACC ATGGAGGCGA GCGAGGATGC CGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTCCAG CAGAAAGACA TTGGCGTGAA ACCTGAGTTC 
AGCTTCAACA TTCCTCGGGC AAAGAGAGAG CTGGGACAGC TGAACAAGTG TACATCCCCA 
CAGCAGAAGC TGCTGTGTCT GCGGAAGGTG GTCCAGCTCA TGACACAGTC TCCCAGCCAG 
AGAGTGAATT TGGAGACCGT CTGTGCTGAT GATCTTCTTT CCGTCCTGTT ATATCTGCTT 
GTGAAAACAG AGATCCCTAA CTGGATGGCG AATTTGAGTT ACATCAAAAA CTTCAGATTT 
AGCAGCTCGC CCAAAGATGA GCTAGGATAC TGCCTGACCT CAGTCGAGGC TGCGATTGAA 
TACATCCGGC AAGGAAGCCT CTCTGCACAG CCGCCTGACG CTGAGGGATT TGGCGACAGA 
CTCTTTCTCA AGCAACGGAT GAACTTATTG TCCCAGATGT CATCAACACC CATCGACTGC 
CTGTTTAAGC ACATCGCTTC TGGCAACCAA AAGGAAGTAG AAAGACTTCT GAGTCAAGAT 
GACCAAGACA AAGACGCCAT GCAAAAGATG TGCCACCCGC TTTGCTCCTG TGAGGACTGT 
GAGAAGCTTG TCTCTGGAAG GCTGAATGAT CCGTCGGTTG TCACTCCTTT CTCCAGAGAT 
GACAGAGGTC AAACACCCCT CCATGTGGCT GCTCTCTGTG GTCAGGCATC CCTCATTGAC 
TTCCTGGTCT CCAAAGGTGC CGTAGTGAAC GCCACAGACT ATCATGGGTC TACTCCCTTA 
CATCTGGCAT GTCAGAAAGG CTTCCAAAGT GTGACGCTGC TGCTGCTACA CTACAAGGCC 
AACACTGAGG TGCAAGACAA CAACGGCAAC ACCCCGCTGC ACCTGGCCTG CACCTACGGG 
CAGGAGGATT GTGTGAAGGC CCTGGTCTAC TATGATGTCC AGGCCTGCAG ATTGGATATT 
GGTAACGAGA AAGGAGACAC AGCCCTGCAC ATTGCAGCTC GCTGGGGTTA CGAGGGCATC 
ATAGAGACGC TGCTGCAGAA TGGAGCCCCA ACAGCAATTC AGAACAGACT GAAGGAAACA 
CCACTCAAGT GTGCACTGAA CTCGAAGATT CTGTCTGCCA TGGAAGCGTA TCATCTATCC 
TCTGACAGGA GGCCAAAGCC TTCTGAGGTC CCTGCACCGT CCCCTACACG CTCTGCGGAT 
TCCATCAGCC AGGGCTCCTC TACCTCCAGC TTTTCTTCGG TATCGGTGAG CTTCAGGCCA 
GAAGAGGTCA AAAAGGACTA CAGGGAGGTA GAAAAGCTTC TCAGAGCAGT TGCCGATGGC 
GACCTAGAAA TGGTGCGATA CCTTTTGGAG TGGACGGAGG ACGACCTAGA CGATATGGAC 
GAAGCCATCA GCACGGTGGA TCTTGAATTC TGTCACCCTT TGTGCCAGTG CCCCAAGTGT 
GCTCCAGCCC AGAAGAAACT GGCAAGGATC CCTGCCAATG GGCTCAGTGT GAATGTGACC 
AACCAGGACG GCTTCTCTCC CCTGCATATG GCTGCCCTGC ATGGCAGGAC AGACCTTGTC 
CCCCTCCTGC TGAAGCATGG AGCCTACTCA GGTGCCAGAA ACACAAGCCA AGCTGTCCCG 
CTCCACCTGG CCTGCCAACA GGGCCACTTT CAGGTGGCAA AATGTCTCTT AGACTCCAAT 
GCAAAACCTA ATAAAAGGGA CCTCAGTGGG AACACGCCCC TCATTTGCGC CTGCTCTGCC 
GGCCATCATG AAGTCGCTGC ACTGTTGCTA CAGCACGGGG CCGCCATCAA TGCTTGCAAC 
AACAAAGGCA ACACGGCCCT GCACGAGGCC GTGATGGGAA GGCACGTCTT GGTGGTGGAA 
CTGCTTCTGT TTTATGGCGC ATCTGTTGAC ATCCTGAACA AGAGGCAGTA CACGGCGGTG 
GACTGTGCAG AGCAGGACTC AAAGATAATG GAGTTGCTGC AAGTCGTCCC AAGTTGCGTT 
GCTGCTTCTC CAAACAACGT CGTTGTCGTC CAGGAGACAG AACACGAGGA TTATGTGACT 
GTGGAGATCA GGAAAAAATG TAAGTGCTTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ankrd27 ( NM_001271264.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001258193.1 Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), mRNA.

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CloneID	ORa10789
Clone ID Related Accession (Same CDS sequence)	NM_001271264.1
Accession Version	NM_001271264.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3162bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-05
Organism	Rattus norvegicus(Norway rat)
Product	ankyrin repeat domain-containing protein 27
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC136661.3, CO574455.1 and AABR07002995.1. On or before Sep 12, 2012 this sequence version replaced XM_341843.5, XM_001079798.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCTGT ATGATGAAGA TCTCCTGAGA AACCCTTTCT ACCTGGCCCT TCAGAAGTGG 
CGCCCCGACT TGTGCAGTAA GGTGGCCCAG ATCCATGGCA TTGTCCTAGT GCCTTGCAGA 
GGAAGCTTGC CGAGCAACGT GCAGGCCTCC TGCCAGTTCG AGTCCTATGT TTTGGTACCC 
ATGGAAGGAC ACTTTCAGAC CTTAGATGGA AAGGCTGTCC TCATAGAAGG AAACAGGGTT 
AAGCTAGGAG CAGGCTTTGC TTGCCTTCTC TCTGTGCCCA TCCTCTTTGA AGAGACTTTC 
TACAACGAAA AGGAAGAGAG TTTCAGCATT CTCTGCATCG CTCATCCTTT GGAGAAGAGG 
GAGACATCAG AAGAACCTTC AGCACCCTCA GATCCTTTCT CTCTGAAAAC CATCAAAGAT 
GTGAGAGAAT TTTTGGGAAG ACACTCAGAG AGATTCGACA AGCACATCGC GTCTTTCCAC 
CGGACATTCC GAGAATGTGA AAGAAAGAGC CTCCGCCATC TCATAGACTC CGTAAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTC AGAGACTCTC ACCTGAAGGT TCTTGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTAGAGA TGTATGTCCA TCATGATATT 
TACGACCTGA TCTTCAAATA CGTGGGGACC ATGGAGGCGA GCGAGGATGC CGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTCCAG CAGAAAGACA TTGGCGTGAA ACCTGAGTTC 
AGCTTCAACA TTCCTCGGGC AAAGAGAGAG CTGGGACAGC TGAACAAGTG TACATCCCCA 
CAGCAGAAGC TGCTGTGTCT GCGGAAGGTG GTCCAGCTCA TGACACAGTC TCCCAGCCAG 
AGAGTGAATT TGGAGACCGT CTGTGCTGAT GATCTTCTTT CCGTCCTGTT ATATCTGCTT 
GTGAAAACAG AGATCCCTAA CTGGATGGCG AATTTGAGTT ACATCAAAAA CTTCAGATTT 
AGCAGCTCGC CCAAAGATGA GCTAGGATAC TGCCTGACCT CAGTCGAGGC TGCGATTGAA 
TACATCCGGC AAGGAAGCCT CTCTGCACAG CCGCCTGACG CTGAGGGATT TGGCGACAGA 
CTCTTTCTCA AGCAACGGAT GAACTTATTG TCCCAGATGT CATCAACACC CATCGACTGC 
CTGTTTAAGC ACATCGCTTC TGGCAACCAA AAGGAAGTAG AAAGACTTCT GAGTCAAGAT 
GACCAAGACA AAGACGCCAT GCAAAAGATG TGCCACCCGC TTTGCTCCTG TGAGGACTGT 
GAGAAGCTTG TCTCTGGAAG GCTGAATGAT CCGTCGGTTG TCACTCCTTT CTCCAGAGAT 
GACAGAGGTC AAACACCCCT CCATGTGGCT GCTCTCTGTG GTCAGGCATC CCTCATTGAC 
TTCCTGGTCT CCAAAGGTGC CGTAGTGAAC GCCACAGACT ATCATGGGTC TACTCCCTTA 
CATCTGGCAT GTCAGAAAGG CTTCCAAAGT GTGACGCTGC TGCTGCTACA CTACAAGGCC 
AACACTGAGG TGCAAGACAA CAACGGCAAC ACCCCGCTGC ACCTGGCCTG CACCTACGGG 
CAGGAGGATT GTGTGAAGGC CCTGGTCTAC TATGATGTCC AGGCCTGCAG ATTGGATATT 
GGTAACGAGA AAGGAGACAC AGCCCTGCAC ATTGCAGCTC GCTGGGGTTA CGAGGGCATC 
ATAGAGACGC TGCTGCAGAA TGGAGCCCCA ACAGCAATTC AGAACAGACT GAAGGAAACA 
CCACTCAAGT GTGCACTGAA CTCGAAGATT CTGTCTGCCA TGGAAGCGTA TCATCTATCC 
TCTGACAGGA GGCCAAAGCC TTCTGAGGTC CCTGCACCGT CCCCTACACG CTCTGCGGAT 
TCCATCAGCC AGGGCTCCTC TACCTCCAGC TTTTCTTCGG TATCGGTGAG CTTCAGGCCA 
GAAGAGGTCA AAAAGGACTA CAGGGAGGTA GAAAAGCTTC TCAGAGCAGT TGCCGATGGC 
GACCTAGAAA TGGTGCGATA CCTTTTGGAG TGGACGGAGG ACGACCTAGA CGATATGGAC 
GAAGCCATCA GCACGGTGGA TCTTGAATTC TGTCACCCTT TGTGCCAGTG CCCCAAGTGT 
GCTCCAGCCC AGAAGAAACT GGCAAGGATC CCTGCCAATG GGCTCAGTGT GAATGTGACC 
AACCAGGACG GCTTCTCTCC CCTGCATATG GCTGCCCTGC ATGGCAGGAC AGACCTTGTC 
CCCCTCCTGC TGAAGCATGG AGCCTACTCA GGTGCCAGAA ACACAAGCCA AGCTGTCCCG 
CTCCACCTGG CCTGCCAACA GGGCCACTTT CAGGTGGCAA AATGTCTCTT AGACTCCAAT 
GCAAAACCTA ATAAAAGGGA CCTCAGTGGG AACACGCCCC TCATTTGCGC CTGCTCTGCC 
GGCCATCATG AAGTCGCTGC ACTGTTGCTA CAGCACGGGG CCGCCATCAA TGCTTGCAAC 
AACAAAGGCA ACACGGCCCT GCACGAGGCC GTGATGGGAA GGCACGTCTT GGTGGTGGAA 
CTGCTTCTGT TTTATGGCGC ATCTGTTGAC ATCCTGAACA AGAGGCAGTA CACGGCGGTG 
GACTGTGCAG AGCAGGACTC AAAGATAATG GAGTTGCTGC AAGTCGTCCC AAGTTGCGTT 
GCTGCTTCTC CAAACAACGT CGTTGTCGTC CAGGAGACAG AACACGAGGA TTATGTGACT 
GTGGAGATCA GGAAAAAATG GAACCCCAAA ATGTACAACC TGCCAGAGGA ACCTTTTCGG 
AGACAGTTTT GCCTCATTAA CCCAGGGGGG CGGTTCCAGG AGAGGACGAA GTCATGGGAG 
ACTATGGGAA GGGATAGAAG TGTACCAGAT CTTACCGGAG GTCTCCAGGA GCCAGAGAAG 
CAAAGAGTCA CAGGGAAGCA GAATGATTTG TCAGACCAGA GCAGGTGTCA GACCTCTGAG 
GAAGGAAACA AGGGGCTCCC TGGGAGGCCT GTGCCGAGAC AGGCTGTTCC AGGACACCGG 
CCAATGGTCC GCAGACACAC AGTCAATGAC GCAGCCATAC TTCAGGTCCC AGAGGTGACT 
GGCCACCTGA CCACCCAAGA GGCTGGTGTT TCTCGGTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001258193.1
CDS	137..3298
Translation	MALYDEDLLRNPFYLALQKWRPDLCSKVAQIHGIVLVPCRGSLPSNVQASCQFESYVLVPMEGHFQTLDGKAVLIEGNRVKLGAGFACLLSVPILFEETFYNEKEESFSILCIAHPLEKRETSEEPSAPSDPFSLKTIKDVREFLGRHSERFDKHIASFHRTFRECERKSLRHLIDSVNALYTKCLQQLLRDSHLKVLAKQEAQMNLMKQAVEMYVHHDIYDLIFKYVGTMEASEDAAFNKITRSLQDLQQKDIGVKPEFSFNIPRAKRELGQLNKCTSPQQKLLCLRKVVQLMTQSPSQRVNLETVCADDLLSVLLYLLVKTEIPNWMANLSYIKNFRFSSSPKDELGYCLTSVEAAIEYIRQGSLSAQPPDAEGFGDRLFLKQRMNLLSQMSSTPIDCLFKHIASGNQKEVERLLSQDDQDKDAMQKMCHPLCSCEDCEKLVSGRLNDPSVVTPFSRDDRGQTPLHVAALCGQASLIDFLVSKGAVVNATDYHGSTPLHLACQKGFQSVTLLLLHYKANTEVQDNNGNTPLHLACTYGQEDCVKALVYYDVQACRLDIGNEKGDTALHIAARWGYEGIIETLLQNGAPTAIQNRLKETPLKCALNSKILSAMEAYHLSSDRRPKPSEVPAPSPTRSADSISQGSSTSSFSSVSVSFRPEEVKKDYREVEKLLRAVADGDLEMVRYLLEWTEDDLDDMDEAISTVDLEFCHPLCQCPKCAPAQKKLARIPANGLSVNVTNQDGFSPLHMAALHGRTDLVPLLLKHGAYSGARNTSQAVPLHLACQQGHFQVAKCLLDSNAKPNKRDLSGNTPLICACSAGHHEVAALLLQHGAAINACNNKGNTALHEAVMGRHVLVVELLLFYGASVDILNKRQYTAVDCAEQDSKIMELLQVVPSCVAASPNNVVVVQETEHEDYVTVEIRKKWNPKMYNLPEEPFRRQFCLINPGGRFQERTKSWETMGRDRSVPDLTGGLQEPEKQRVTGKQNDLSDQSRCQTSEEGNKGLPGRPVPRQAVPGHRPMVRRHTVNDAAILQVPEVTGHLTTQEAGVSRS

Target ORF information:

RefSeq Version	NM_001271264.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ankyrin repeat domain 27 (Ankrd27), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001271264.1

ORF Insert Sequence:

ATGGCTCTGT ATGATGAAGA TCTCCTGAGA AACCCTTTCT ACCTGGCCCT TCAGAAGTGG 
CGCCCCGACT TGTGCAGTAA GGTGGCCCAG ATCCATGGCA TTGTCCTAGT GCCTTGCAGA 
GGAAGCTTGC CGAGCAACGT GCAGGCCTCC TGCCAGTTCG AGTCCTATGT TTTGGTACCC 
ATGGAAGGAC ACTTTCAGAC CTTAGATGGA AAGGCTGTCC TCATAGAAGG AAACAGGGTT 
AAGCTAGGAG CAGGCTTTGC TTGCCTTCTC TCTGTGCCCA TCCTCTTTGA AGAGACTTTC 
TACAACGAAA AGGAAGAGAG TTTCAGCATT CTCTGCATCG CTCATCCTTT GGAGAAGAGG 
GAGACATCAG AAGAACCTTC AGCACCCTCA GATCCTTTCT CTCTGAAAAC CATCAAAGAT 
GTGAGAGAAT TTTTGGGAAG ACACTCAGAG AGATTCGACA AGCACATCGC GTCTTTCCAC 
CGGACATTCC GAGAATGTGA AAGAAAGAGC CTCCGCCATC TCATAGACTC CGTAAATGCT 
CTCTACACCA AATGCCTCCA GCAGCTTCTC AGAGACTCTC ACCTGAAGGT TCTTGCCAAG 
CAGGAGGCCC AGATGAACCT GATGAAGCAG GCAGTAGAGA TGTATGTCCA TCATGATATT 
TACGACCTGA TCTTCAAATA CGTGGGGACC ATGGAGGCGA GCGAGGATGC CGCCTTTAAC 
AAAATCACAA GAAGCCTTCA AGATCTCCAG CAGAAAGACA TTGGCGTGAA ACCTGAGTTC 
AGCTTCAACA TTCCTCGGGC AAAGAGAGAG CTGGGACAGC TGAACAAGTG TACATCCCCA 
CAGCAGAAGC TGCTGTGTCT GCGGAAGGTG GTCCAGCTCA TGACACAGTC TCCCAGCCAG 
AGAGTGAATT TGGAGACCGT CTGTGCTGAT GATCTTCTTT CCGTCCTGTT ATATCTGCTT 
GTGAAAACAG AGATCCCTAA CTGGATGGCG AATTTGAGTT ACATCAAAAA CTTCAGATTT 
AGCAGCTCGC CCAAAGATGA GCTAGGATAC TGCCTGACCT CAGTCGAGGC TGCGATTGAA 
TACATCCGGC AAGGAAGCCT CTCTGCACAG CCGCCTGACG CTGAGGGATT TGGCGACAGA 
CTCTTTCTCA AGCAACGGAT GAACTTATTG TCCCAGATGT CATCAACACC CATCGACTGC 
CTGTTTAAGC ACATCGCTTC TGGCAACCAA AAGGAAGTAG AAAGACTTCT GAGTCAAGAT 
GACCAAGACA AAGACGCCAT GCAAAAGATG TGCCACCCGC TTTGCTCCTG TGAGGACTGT 
GAGAAGCTTG TCTCTGGAAG GCTGAATGAT CCGTCGGTTG TCACTCCTTT CTCCAGAGAT 
GACAGAGGTC AAACACCCCT CCATGTGGCT GCTCTCTGTG GTCAGGCATC CCTCATTGAC 
TTCCTGGTCT CCAAAGGTGC CGTAGTGAAC GCCACAGACT ATCATGGGTC TACTCCCTTA 
CATCTGGCAT GTCAGAAAGG CTTCCAAAGT GTGACGCTGC TGCTGCTACA CTACAAGGCC 
AACACTGAGG TGCAAGACAA CAACGGCAAC ACCCCGCTGC ACCTGGCCTG CACCTACGGG 
CAGGAGGATT GTGTGAAGGC CCTGGTCTAC TATGATGTCC AGGCCTGCAG ATTGGATATT 
GGTAACGAGA AAGGAGACAC AGCCCTGCAC ATTGCAGCTC GCTGGGGTTA CGAGGGCATC 
ATAGAGACGC TGCTGCAGAA TGGAGCCCCA ACAGCAATTC AGAACAGACT GAAGGAAACA 
CCACTCAAGT GTGCACTGAA CTCGAAGATT CTGTCTGCCA TGGAAGCGTA TCATCTATCC 
TCTGACAGGA GGCCAAAGCC TTCTGAGGTC CCTGCACCGT CCCCTACACG CTCTGCGGAT 
TCCATCAGCC AGGGCTCCTC TACCTCCAGC TTTTCTTCGG TATCGGTGAG CTTCAGGCCA 
GAAGAGGTCA AAAAGGACTA CAGGGAGGTA GAAAAGCTTC TCAGAGCAGT TGCCGATGGC 
GACCTAGAAA TGGTGCGATA CCTTTTGGAG TGGACGGAGG ACGACCTAGA CGATATGGAC 
GAAGCCATCA GCACGGTGGA TCTTGAATTC TGTCACCCTT TGTGCCAGTG CCCCAAGTGT 
GCTCCAGCCC AGAAGAAACT GGCAAGGATC CCTGCCAATG GGCTCAGTGT GAATGTGACC 
AACCAGGACG GCTTCTCTCC CCTGCATATG GCTGCCCTGC ATGGCAGGAC AGACCTTGTC 
CCCCTCCTGC TGAAGCATGG AGCCTACTCA GGTGCCAGAA ACACAAGCCA AGCTGTCCCG 
CTCCACCTGG CCTGCCAACA GGGCCACTTT CAGGTGGCAA AATGTCTCTT AGACTCCAAT 
GCAAAACCTA ATAAAAGGGA CCTCAGTGGG AACACGCCCC TCATTTGCGC CTGCTCTGCC 
GGCCATCATG AAGTCGCTGC ACTGTTGCTA CAGCACGGGG CCGCCATCAA TGCTTGCAAC 
AACAAAGGCA ACACGGCCCT GCACGAGGCC GTGATGGGAA GGCACGTCTT GGTGGTGGAA 
CTGCTTCTGT TTTATGGCGC ATCTGTTGAC ATCCTGAACA AGAGGCAGTA CACGGCGGTG 
GACTGTGCAG AGCAGGACTC AAAGATAATG GAGTTGCTGC AAGTCGTCCC AAGTTGCGTT 
GCTGCTTCTC CAAACAACGT CGTTGTCGTC CAGGAGACAG AACACGAGGA TTATGTGACT 
GTGGAGATCA GGAAAAAATG GAACCCCAAA ATGTACAACC TGCCAGAGGA ACCTTTTCGG 
AGACAGTTTT GCCTCATTAA CCCAGGGGGG CGGTTCCAGG AGAGGACGAA GTCATGGGAG 
ACTATGGGAA GGGATAGAAG TGTACCAGAT CTTACCGGAG GTCTCCAGGA GCCAGAGAAG 
CAAAGAGTCA CAGGGAAGCA GAATGATTTG TCAGACCAGA GCAGGTGTCA GACCTCTGAG 
GAAGGAAACA AGGGGCTCCC TGGGAGGCCT GTGCCGAGAC AGGCTGTTCC AGGACACCGG 
CCAATGGTCC GCAGACACAC AGTCAATGAC GCAGCCATAC TTCAGGTCCC AGAGGTGACT 
GGCCACCTGA CCACCCAAGA GGCTGGTGTT TCTCGGTCTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Retromer and sorting nexins in endosomal sorting.
Biochemical Society transactions43(1)33-47(2015 Feb)
Gallon M,Cullen PJ

GWAS identifies four novel eosinophilic esophagitis loci.
Nature communications55593(2014 Nov)
Sleiman PM,Wang ML,Cianferoni A,Aceves S,Gonsalves N,Nadeau K,Bredenoord AJ,Furuta GT,Spergel JM,Hakonarson H

VARP is recruited on to endosomes by direct interaction with retromer, where together they function in export to the cell surface.
Developmental cell29(5)591-606(2014 Jun)
Hesketh GG,P?rez-Dorado I,Jackson LP,Wartosch L,Sch?fer IB,Gray SR,McCoy AJ,Zeldin OB,Garman EF,Harbour ME,Evans PR,Seaman MNJ,Luzio JP,Owen DJ

The Rab21-GEF activity of Varp, but not its Rab32/38 effector function, is required for dendrite formation in melanocytes.
Molecular biology of the cell23(4)669-78(2012 Feb)
Ohbayashi N,Yatsu A,Tamura K,Fukuda M

Evaluation of genotoxic potential of neurotoxin anatoxin-a with the use of umuC test.
Neuro endocrinology letters31 Suppl 216-20(2010)
Sieroslawska A,Rymuszka A

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Ankrd27 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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