DGKI cDNA ORF clone, Canis lupus familiaris(dog)

The following DGKI gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DGKI cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe293859 XM_022403757.1
Latest version!
Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe293860 XM_022403758.1
Latest version!
Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe293861 XM_022403759.1
Latest version!
Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe293862 XM_022403760.1
Latest version!
Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe40463 XM_014119884.1
Latest version!
Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe40464 XM_014119885.1
Latest version!
Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OCe40461 XM_014119882.1
Latest version!
Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OCe40462 XM_014119883.1
Latest version!
Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe293859
    Clone ID Related Accession (Same CDS sequence) XM_022403757.1
    Accession Version XM_022403757.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2931bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product diacylglycerol kinase iota isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTATGGGT CCAGAAATCA AACTACAAAT ATAATTTCTC CAGCTCTTAT TTATATTCAA 
    AGTTGTGATC ATAATATACA GGAGAATGCT GTGAACGGAG AGCACCTGTG GCTGGAGACC
    AATGTCTCAG GAGACTTATG CTACCTGGGA GAAGAGAACT GCCAAGTCAG ATTTGCAAAA
    TCCGCCCTGA GGAGGAAGTG TGCGGTCTGT AAAATCGTCG TCCACGCTGC CTGCATTGAA
    CAGCTAGAGA AGATTAATTT CAGATGTAAG CCAACATTTC GAGAAGGGGG CTCAAGATCA
    CCAAGAGAAA ACTTTGTGCG TCATCACTGG GTGCACAGGC GTCGGCAGGA GGGAAAATGT
    AAGCAGTGTG GTAAGGGCTT CCAGCAAAAA TTCTCCTTCC ACAGTAAAGA GATTGTGGCT
    ATCAGCTGTT CCTGGTGCAA GCAGGCGTTT CACAATAAGG TGACCTGCTT CATGCTGCAT
    CACATTGAGG AACCCTGCTC CCTGGGGGCT CATGCTGCTG TTATCGTCCC GCCCACCTGG
    ATCATTAAGG TCAAGAAGCC TCAGGTGATC TCCATACCAG GAGGTGAACT TGAACTTGGT
    TGGGCGGGAG ACGGACCTGG TTGGGCGGGA GACTGGAGCC CCTCAGAGAA TTTGGGATTC
    TTCCATTTTT ACTGGGAGGG TTGGCAGAAC TCATTGAAGG CTTCAAATCG GAAGAAGAAG
    AGAACAAGCT TTAAAAGAAA AGCCAGTAAA AGAGGAACTG AACAGGAAAA CAAAGGTCGT
    CCTTTTGTGA TAAAACCTAT CTCTTCTCCT CTCATGAAAC CCTTGCTTGT GTTTGTGAAC
    CCCAAGAGTG GAGGCAACCA GGGAACCAAA GTCCTGCAGA TGTTCATGTG GTACCTGAAT
    CCTCGGCAGG TCTTTGATCT TTCTCAGGAA GGGCCAAAAG ATGCGCTTGA GTTATATAGG
    AAAGTACCAA ATCTGCGAAT TCTGGCTTGT GGAGGGGATG GAACGGTGGG CTGGATCCTT
    TCTATTCTGG ATGAACTACA GCTGAGCCCT CAGCCTCCCG TCGGGGTCCT TCCTCTGGGG
    ACTGGGAATG ACCTGGCTCG AACTCTCAAC TGGGGAGGGG GCTACACTGA TGAACCTGTC
    TCTAAGATCC TTTGCCAAGT AGAAGATGGG ACAATCGTAC AGCTGGATCG TTGGAACCTC
    CATGTGGAAA GAAACCCAGA CTTGCCTCCA GAAGAACTTG AAGATGGCGT ATGTAAGCTC
    CCTCTGAATG TTTTCAATAA TTACTTCAGC CTTGGATTTG ATGCCCACGT CACACTGGAG
    TTCCATGAAT CCAGAGAAGC AAATCCAGAG AAATTCAACA GTCGTTTTCG AAATAAAATG
    TTCTATGCAG GGGCAGCTTT TTCTGATTTC CTACAGAGAA GTTCTAGAGA TCTATCCAAA
    CATGTTAAAG TTGTTTGTGA TGGAACAGAT CTCACTCCAA AGATTCAGGA ACTGAAGTTC
    CAGTGTATAG TATTTTTAAA TATACCCAGA TATTGTGCTG GCACAATGCC CTGGGGGAAC
    CCAGGAGATC ACCATGATTT TGAACCTCAG CGTCATGATG ATGGTTATAT TGAAGTCATT
    GGGTTCACTA TGGCCTCTTT GGCTGCCCTG CAAGTTGGGG GCCATGGGGA AAGGCTACAC
    CAGTGTCGGG AGGTCATGCT GCTCACTTAC AAATCCATCC CCATGCAAGT GGATGGGGAG
    CCCTGTAGGT TGGCCCCGGC TATGATTCGG ATCTCCTTGA GGAATCAAGC CAACATGGTA
    CAGAAGAGCA AGAGGAGAAC ATCCATGCCT TTACTCAATG ATATGCACCA GGTGTCCGCT
    GGCGACCTGC GGAGAGTCTC GGCTCCCCCT GGCTCCTTCA CCACTCCCCA ATCTGTCCCA
    GACCGCCTGA GGATCCGGGT GAACAAAATC AGTTTACAAG ACTATGAAGG ATTCCACTAT
    GACAAAGAGA AACTTCGGGA AGCTTCTATC CCTCTGGGTA TTCTAGTTGT GCGTGGAGAC
    TGTGATTTGG AGACTTGCCG TATGTACATA GATCGCCTAC AGGAGGACCT GCAGTCAGTT
    TCTTCTGGCT CCCAGAGAGT TCATTACCAG GACCATGAAA CCTCCTTCCC CAGGGCGCTC
    TCAGCTCAGA GACTCTCCCC ACGGTGGTGC TTTCTAGATG CAACTTCTGC TGATCGCTTT
    TACCGAATAG ACAGATCTCA GGAACATTTG CACTTTGTGA TGGAGATTTC CCAAGATGAG
    ATTTTTATTC TGGACCCAGA TATGGTGTTG TCACAGCAGG CAGGGACACC TCCAGGAATG
    CCTGACCTGG TGGTGGAGCA AGCCTCAGGG CTGTCAGAAT GGTGGAATCC TGCCCTACGG
    AAGCGTATCC TGAGCGACAG TGGTCTGGGA ACGATTACTC CGCATTATGA GGACTCCGAT
    TTGAGGGATC TCAGCCACTC ACGTGTGCTA CAGTCACCAG TCTCTTCAGA AGATCACGCA
    ATTTTGCAGG CAGTAATAGC TGGTGATCTT ATGAAGCTAA TGGAAAGCTA TAAAAATGGA
    GGCAGTCTGC TGATCCAGGG GCCGGACCAC TGCTCCCTCC TTCACCATGC AGCTAAAACT
    GGCCACGGGG AGATCGTAAA ATATATCCTG GACCACGGAC CTTCTGAGTT ATTGGATATG
    GCAGACAGTG AAACGGGTGA GACCGCACTG CACAAGGCTG CCTGCCAGCG GAACCGGGCT
    GTGTGCCAGC TTCTGGTGGA TGCGGGAGCA TCTCTGAGAA AGACGGACTC CAAGGGTAAG
    ACCCCTCAAG ACAGAGCACA GCAAGCTGGA GACCCAGATT TGGCTGCTTA CCTAGAAAGC
    TGCCAGAACT ATAAGATCAT TGGCCATGAG GACCTGGAAA CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259465.1
    CDS290..3220
    Translation

    Target ORF information:

    RefSeq Version XM_022403757.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403757.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGTATGGGT CCAGAAATCA AACTACAAAT ATAATTTCTC CAGCTCTTAT TTATATTCAA 
    AGTTGTGATC ATAATATACA GGAGAATGCT GTGAACGGAG AGCACCTGTG GCTGGAGACC
    AATGTCTCAG GAGACTTATG CTACCTGGGA GAAGAGAACT GCCAAGTCAG ATTTGCAAAA
    TCCGCCCTGA GGAGGAAGTG TGCGGTCTGT AAAATCGTCG TCCACGCTGC CTGCATTGAA
    CAGCTAGAGA AGATTAATTT CAGATGTAAG CCAACATTTC GAGAAGGGGG CTCAAGATCA
    CCAAGAGAAA ACTTTGTGCG TCATCACTGG GTGCACAGGC GTCGGCAGGA GGGAAAATGT
    AAGCAGTGTG GTAAGGGCTT CCAGCAAAAA TTCTCCTTCC ACAGTAAAGA GATTGTGGCT
    ATCAGCTGTT CCTGGTGCAA GCAGGCGTTT CACAATAAGG TGACCTGCTT CATGCTGCAT
    CACATTGAGG AACCCTGCTC CCTGGGGGCT CATGCTGCTG TTATCGTCCC GCCCACCTGG
    ATCATTAAGG TCAAGAAGCC TCAGGTGATC TCCATACCAG GAGGTGAACT TGAACTTGGT
    TGGGCGGGAG ACGGACCTGG TTGGGCGGGA GACTGGAGCC CCTCAGAGAA TTTGGGATTC
    TTCCATTTTT ACTGGGAGGG TTGGCAGAAC TCATTGAAGG CTTCAAATCG GAAGAAGAAG
    AGAACAAGCT TTAAAAGAAA AGCCAGTAAA AGAGGAACTG AACAGGAAAA CAAAGGTCGT
    CCTTTTGTGA TAAAACCTAT CTCTTCTCCT CTCATGAAAC CCTTGCTTGT GTTTGTGAAC
    CCCAAGAGTG GAGGCAACCA GGGAACCAAA GTCCTGCAGA TGTTCATGTG GTACCTGAAT
    CCTCGGCAGG TCTTTGATCT TTCTCAGGAA GGGCCAAAAG ATGCGCTTGA GTTATATAGG
    AAAGTACCAA ATCTGCGAAT TCTGGCTTGT GGAGGGGATG GAACGGTGGG CTGGATCCTT
    TCTATTCTGG ATGAACTACA GCTGAGCCCT CAGCCTCCCG TCGGGGTCCT TCCTCTGGGG
    ACTGGGAATG ACCTGGCTCG AACTCTCAAC TGGGGAGGGG GCTACACTGA TGAACCTGTC
    TCTAAGATCC TTTGCCAAGT AGAAGATGGG ACAATCGTAC AGCTGGATCG TTGGAACCTC
    CATGTGGAAA GAAACCCAGA CTTGCCTCCA GAAGAACTTG AAGATGGCGT ATGTAAGCTC
    CCTCTGAATG TTTTCAATAA TTACTTCAGC CTTGGATTTG ATGCCCACGT CACACTGGAG
    TTCCATGAAT CCAGAGAAGC AAATCCAGAG AAATTCAACA GTCGTTTTCG AAATAAAATG
    TTCTATGCAG GGGCAGCTTT TTCTGATTTC CTACAGAGAA GTTCTAGAGA TCTATCCAAA
    CATGTTAAAG TTGTTTGTGA TGGAACAGAT CTCACTCCAA AGATTCAGGA ACTGAAGTTC
    CAGTGTATAG TATTTTTAAA TATACCCAGA TATTGTGCTG GCACAATGCC CTGGGGGAAC
    CCAGGAGATC ACCATGATTT TGAACCTCAG CGTCATGATG ATGGTTATAT TGAAGTCATT
    GGGTTCACTA TGGCCTCTTT GGCTGCCCTG CAAGTTGGGG GCCATGGGGA AAGGCTACAC
    CAGTGTCGGG AGGTCATGCT GCTCACTTAC AAATCCATCC CCATGCAAGT GGATGGGGAG
    CCCTGTAGGT TGGCCCCGGC TATGATTCGG ATCTCCTTGA GGAATCAAGC CAACATGGTA
    CAGAAGAGCA AGAGGAGAAC ATCCATGCCT TTACTCAATG ATATGCACCA GGTGTCCGCT
    GGCGACCTGC GGAGAGTCTC GGCTCCCCCT GGCTCCTTCA CCACTCCCCA ATCTGTCCCA
    GACCGCCTGA GGATCCGGGT GAACAAAATC AGTTTACAAG ACTATGAAGG ATTCCACTAT
    GACAAAGAGA AACTTCGGGA AGCTTCTATC CCTCTGGGTA TTCTAGTTGT GCGTGGAGAC
    TGTGATTTGG AGACTTGCCG TATGTACATA GATCGCCTAC AGGAGGACCT GCAGTCAGTT
    TCTTCTGGCT CCCAGAGAGT TCATTACCAG GACCATGAAA CCTCCTTCCC CAGGGCGCTC
    TCAGCTCAGA GACTCTCCCC ACGGTGGTGC TTTCTAGATG CAACTTCTGC TGATCGCTTT
    TACCGAATAG ACAGATCTCA GGAACATTTG CACTTTGTGA TGGAGATTTC CCAAGATGAG
    ATTTTTATTC TGGACCCAGA TATGGTGTTG TCACAGCAGG CAGGGACACC TCCAGGAATG
    CCTGACCTGG TGGTGGAGCA AGCCTCAGGG CTGTCAGAAT GGTGGAATCC TGCCCTACGG
    AAGCGTATCC TGAGCGACAG TGGTCTGGGA ACGATTACTC CGCATTATGA GGACTCCGAT
    TTGAGGGATC TCAGCCACTC ACGTGTGCTA CAGTCACCAG TCTCTTCAGA AGATCACGCA
    ATTTTGCAGG CAGTAATAGC TGGTGATCTT ATGAAGCTAA TGGAAAGCTA TAAAAATGGA
    GGCAGTCTGC TGATCCAGGG GCCGGACCAC TGCTCCCTCC TTCACCATGC AGCTAAAACT
    GGCCACGGGG AGATCGTAAA ATATATCCTG GACCACGGAC CTTCTGAGTT ATTGGATATG
    GCAGACAGTG AAACGGGTGA GACCGCACTG CACAAGGCTG CCTGCCAGCG GAACCGGGCT
    GTGTGCCAGC TTCTGGTGGA TGCGGGAGCA TCTCTGAGAA AGACGGACTC CAAGGGTAAG
    ACCCCTCAAG ACAGAGCACA GCAAGCTGGA GACCCAGATT TGGCTGCTTA CCTAGAAAGC
    TGCCAGAACT ATAAGATCAT TGGCCATGAG GACCTGGAAA CTGCTGTTTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293860
    Clone ID Related Accession (Same CDS sequence) XM_022403758.1
    Accession Version XM_022403758.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2745bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product diacylglycerol kinase iota isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTATGGGT CCAGAAATCA AACTACAAAT ATAATTTCTC CAGCTCTTAT TTATATTCAA 
    AGTTGTGATC ATAATATACA GGAGAATGCT GTGAACGGAG AGCACCTGTG GCTGGAGACC
    AATGTCTCAG GAGACTTATG CTACCTGGGA GAAGAGAACT GCCAAGTCAG ATTTGCAAAA
    TCCGCCCTGA GGAGGAAGTG TGCGGTCTGT AAAATCGTCG TCCACGCTGC CTGCATTGAA
    CAGCTAGAGA AGATTAATTT CAGATGTAAG CCAACATTTC GAGAAGGGGG CTCAAGATCA
    CCAAGAGAAA ACTTTGTGCG TCATCACTGG GTGCACAGGC GTCGGCAGGA GGGAAAATGT
    AAGCAGTGTG GTAAGGGCTT CCAGCAAAAA TTCTCCTTCC ACAGTAAAGA GATTGTGGCT
    ATCAGCTGTT CCTGGTGCAA GCAGGCGTTT CACAATAAGG TGACCTGCTT CATGCTGCAT
    CACATTGAGG AACCCTGCTC CCTGGGGGCT CATGCTGCTG TTATCGTCCC GCCCACCTGG
    ATCATTAAGG TCAAGAAGCC TCAGAACTCA TTGAAGGCTT CAAATCGGAA GAAGAAGAGA
    ACAAGCTTTA AAAGAAAAGC CAGTAAAAGA GGAACTGAAC AGGAAAACAA AGGTCGTCCT
    TTTGTGATAA AACCTATCTC TTCTCCTCTC ATGAAACCCT TGCTTGTGTT TGTGAACCCC
    AAGAGTGGAG GCAACCAGGG AACCAAAGTC CTGCAGATGT TCATGTGGTA CCTGAATCCT
    CGGCAGGTCT TTGATCTTTC TCAGGAAGGG CCAAAAGATG CGCTTGAGTT ATATAGGAAA
    GTACCAAATC TGCGAATTCT GGCTTGTGGA GGGGATGGAA CGGTGGGCTG GATCCTTTCT
    ATTCTGGATG AACTACAGCT GAGCCCTCAG CCTCCCGTCG GGGTCCTTCC TCTGGGGACT
    GGGAATGACC TGGCTCGAAC TCTCAACTGG GGAGGGGGCT ACACTGATGA ACCTGTCTCT
    AAGATCCTTT GCCAAGTAGA AGATGGGACA ATCGTACAGC TGGATCGTTG GAACCTCCAT
    GTGGAAAGAA ACCCAGACTT GCCTCCAGAA GAACTTGAAG ATGGCGTATG TAAGCTCCCT
    CTGAATGTTT TCAATAATTA CTTCAGCCTT GGATTTGATG CCCACGTCAC ACTGGAGTTC
    CATGAATCCA GAGAAGCAAA TCCAGAGAAA TTCAACAGTC GTTTTCGAAA TAAAATGTTC
    TATGCAGGGG CAGCTTTTTC TGATTTCCTA CAGAGAAGTT CTAGAGATCT ATCCAAACAT
    GTTAAAGTTG TTTGTGATGG AACAGATCTC ACTCCAAAGA TTCAGGAACT GAAGTTCCAG
    TGTATAGTAT TTTTAAATAT ACCCAGATAT TGTGCTGGCA CAATGCCCTG GGGGAACCCA
    GGAGATCACC ATGATTTTGA ACCTCAGCGT CATGATGATG GTTATATTGA AGTCATTGGG
    TTCACTATGG CCTCTTTGGC TGCCCTGCAA GTTGGGGGCC ATGGGGAAAG GCTACACCAG
    TGTCGGGAGG TCATGCTGCT CACTTACAAA TCCATCCCCA TGCAAGTGGA TGGGGAGCCC
    TGTAGGTTGG CCCCGGCTAT GATTCGGATC TCCTTGAGGA ATCAAGCCAA CATGGTACAG
    AAGAGCAAGA GGAGAACATC CATGCCTTTA CTCAATGATC CCCAATCTGT CCCAGACCGC
    CTGAGGATCC GGGTGAACAA AATCAGTTTA CAAGACTATG AAGGATTCCA CTATGACAAA
    GAGAAACTTC GGGAAGCTTC TATCCCTCTG GGTATTCTAG TTGTGCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGATCGC CTACAGGAGG ACCTGCAGTC AGTTTCTTCT
    GGCTCCCAGA GAGTTCATTA CCAGGACCAT GAAACCTCCT TCCCCAGGGC GCTCTCAGCT
    CAGAGACTCT CCCCACGGTG GTGCTTTCTA GATGCAACTT CTGCTGATCG CTTTTACCGA
    ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCCCAAGA TGAGATTTTT
    ATTCTGGACC CAGATATGGT GTTGTCACAG CAGGCAGGGA CACCTCCAGG AATGCCTGAC
    CTGGTGGTGG AGCAAGCCTC AGGGCTGTCA GAATGGTGGA ATCCTGCCCT ACGGAAGCGT
    ATCCTGAGCG ACAGTGGTCT GGGAACGATT ACTCCGCATT ATGAGGACTC CGATTTGAGG
    GATCTCAGCC ACTCACGTGT GCTACAGTCA CCAGTCTCTT CAGAAGATCA CGCAATTTTG
    CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATGGAAA GCTATAAAAA TGGAGGCAGT
    CTGCTGATCC AGGGGCCGGA CCACTGCTCC CTCCTTCACC ATGCAGCTAA AACTGGCCAC
    GGGGAGATCG TAAAATATAT CCTGGACCAC GGACCTTCTG AGTTATTGGA TATGGCAGAC
    AGTGAAACGG GTGAGACCGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC
    CAGCTTCTGG TGGATGCGGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACCCCT
    CAAGACAGAG CACAGCAAGC TGGAGACCCA GATTTGGCTG CTTACCTAGA AAGCTGCCAG
    AACTATAAGA TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259466.1
    CDS287..3031
    Translation

    Target ORF information:

    RefSeq Version XM_022403758.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403758.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGTATGGGT CCAGAAATCA AACTACAAAT ATAATTTCTC CAGCTCTTAT TTATATTCAA 
    AGTTGTGATC ATAATATACA GGAGAATGCT GTGAACGGAG AGCACCTGTG GCTGGAGACC
    AATGTCTCAG GAGACTTATG CTACCTGGGA GAAGAGAACT GCCAAGTCAG ATTTGCAAAA
    TCCGCCCTGA GGAGGAAGTG TGCGGTCTGT AAAATCGTCG TCCACGCTGC CTGCATTGAA
    CAGCTAGAGA AGATTAATTT CAGATGTAAG CCAACATTTC GAGAAGGGGG CTCAAGATCA
    CCAAGAGAAA ACTTTGTGCG TCATCACTGG GTGCACAGGC GTCGGCAGGA GGGAAAATGT
    AAGCAGTGTG GTAAGGGCTT CCAGCAAAAA TTCTCCTTCC ACAGTAAAGA GATTGTGGCT
    ATCAGCTGTT CCTGGTGCAA GCAGGCGTTT CACAATAAGG TGACCTGCTT CATGCTGCAT
    CACATTGAGG AACCCTGCTC CCTGGGGGCT CATGCTGCTG TTATCGTCCC GCCCACCTGG
    ATCATTAAGG TCAAGAAGCC TCAGAACTCA TTGAAGGCTT CAAATCGGAA GAAGAAGAGA
    ACAAGCTTTA AAAGAAAAGC CAGTAAAAGA GGAACTGAAC AGGAAAACAA AGGTCGTCCT
    TTTGTGATAA AACCTATCTC TTCTCCTCTC ATGAAACCCT TGCTTGTGTT TGTGAACCCC
    AAGAGTGGAG GCAACCAGGG AACCAAAGTC CTGCAGATGT TCATGTGGTA CCTGAATCCT
    CGGCAGGTCT TTGATCTTTC TCAGGAAGGG CCAAAAGATG CGCTTGAGTT ATATAGGAAA
    GTACCAAATC TGCGAATTCT GGCTTGTGGA GGGGATGGAA CGGTGGGCTG GATCCTTTCT
    ATTCTGGATG AACTACAGCT GAGCCCTCAG CCTCCCGTCG GGGTCCTTCC TCTGGGGACT
    GGGAATGACC TGGCTCGAAC TCTCAACTGG GGAGGGGGCT ACACTGATGA ACCTGTCTCT
    AAGATCCTTT GCCAAGTAGA AGATGGGACA ATCGTACAGC TGGATCGTTG GAACCTCCAT
    GTGGAAAGAA ACCCAGACTT GCCTCCAGAA GAACTTGAAG ATGGCGTATG TAAGCTCCCT
    CTGAATGTTT TCAATAATTA CTTCAGCCTT GGATTTGATG CCCACGTCAC ACTGGAGTTC
    CATGAATCCA GAGAAGCAAA TCCAGAGAAA TTCAACAGTC GTTTTCGAAA TAAAATGTTC
    TATGCAGGGG CAGCTTTTTC TGATTTCCTA CAGAGAAGTT CTAGAGATCT ATCCAAACAT
    GTTAAAGTTG TTTGTGATGG AACAGATCTC ACTCCAAAGA TTCAGGAACT GAAGTTCCAG
    TGTATAGTAT TTTTAAATAT ACCCAGATAT TGTGCTGGCA CAATGCCCTG GGGGAACCCA
    GGAGATCACC ATGATTTTGA ACCTCAGCGT CATGATGATG GTTATATTGA AGTCATTGGG
    TTCACTATGG CCTCTTTGGC TGCCCTGCAA GTTGGGGGCC ATGGGGAAAG GCTACACCAG
    TGTCGGGAGG TCATGCTGCT CACTTACAAA TCCATCCCCA TGCAAGTGGA TGGGGAGCCC
    TGTAGGTTGG CCCCGGCTAT GATTCGGATC TCCTTGAGGA ATCAAGCCAA CATGGTACAG
    AAGAGCAAGA GGAGAACATC CATGCCTTTA CTCAATGATC CCCAATCTGT CCCAGACCGC
    CTGAGGATCC GGGTGAACAA AATCAGTTTA CAAGACTATG AAGGATTCCA CTATGACAAA
    GAGAAACTTC GGGAAGCTTC TATCCCTCTG GGTATTCTAG TTGTGCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGATCGC CTACAGGAGG ACCTGCAGTC AGTTTCTTCT
    GGCTCCCAGA GAGTTCATTA CCAGGACCAT GAAACCTCCT TCCCCAGGGC GCTCTCAGCT
    CAGAGACTCT CCCCACGGTG GTGCTTTCTA GATGCAACTT CTGCTGATCG CTTTTACCGA
    ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCCCAAGA TGAGATTTTT
    ATTCTGGACC CAGATATGGT GTTGTCACAG CAGGCAGGGA CACCTCCAGG AATGCCTGAC
    CTGGTGGTGG AGCAAGCCTC AGGGCTGTCA GAATGGTGGA ATCCTGCCCT ACGGAAGCGT
    ATCCTGAGCG ACAGTGGTCT GGGAACGATT ACTCCGCATT ATGAGGACTC CGATTTGAGG
    GATCTCAGCC ACTCACGTGT GCTACAGTCA CCAGTCTCTT CAGAAGATCA CGCAATTTTG
    CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATGGAAA GCTATAAAAA TGGAGGCAGT
    CTGCTGATCC AGGGGCCGGA CCACTGCTCC CTCCTTCACC ATGCAGCTAA AACTGGCCAC
    GGGGAGATCG TAAAATATAT CCTGGACCAC GGACCTTCTG AGTTATTGGA TATGGCAGAC
    AGTGAAACGG GTGAGACCGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC
    CAGCTTCTGG TGGATGCGGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACCCCT
    CAAGACAGAG CACAGCAAGC TGGAGACCCA GATTTGGCTG CTTACCTAGA AAGCTGCCAG
    AACTATAAGA TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293861
    Clone ID Related Accession (Same CDS sequence) XM_022403759.1
    Accession Version XM_022403759.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2337bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product diacylglycerol kinase iota isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCATC ACATTGAGGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATCGTCCCG 
    CCCACCTGGA TCATTAAGGT CAAGAAGCCT CAGAACTCAT TGAAGGCTTC AAATCGGAAG
    AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GAACTGAACA GGAAAACAAA
    GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTGTTT
    GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC
    CTGAATCCTC GGCAGGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAGTTA
    TATAGGAAAG TACCAAATCT GCGAATTCTG GCTTGTGGAG GGGATGGAAC GGTGGGCTGG
    ATCCTTTCTA TTCTGGATGA ACTACAGCTG AGCCCTCAGC CTCCCGTCGG GGTCCTTCCT
    CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA
    CCTGTCTCTA AGATCCTTTG CCAAGTAGAA GATGGGACAA TCGTACAGCT GGATCGTTGG
    AACCTCCATG TGGAAAGAAA CCCAGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT
    AAGCTCCCTC TGAATGTTTT CAATAATTAC TTCAGCCTTG GATTTGATGC CCACGTCACA
    CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT
    AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGTTC TAGAGATCTA
    TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CTCCAAAGAT TCAGGAACTG
    AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG
    GGGAACCCAG GAGATCACCA TGATTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA
    GTCATTGGGT TCACTATGGC CTCTTTGGCT GCCCTGCAAG TTGGGGGCCA TGGGGAAAGG
    CTACACCAGT GTCGGGAGGT CATGCTGCTC ACTTACAAAT CCATCCCCAT GCAAGTGGAT
    GGGGAGCCCT GTAGGTTGGC CCCGGCTATG ATTCGGATCT CCTTGAGGAA TCAAGCCAAC
    ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATAT GCACCAGGTG
    TCCGCTGGCG ACCTGCGGAG AGTCTCGGCT CCCCCTGGCT CCTTCACCAC TCCCCAATCT
    GTCCCAGACC GCCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC
    CACTATGACA AAGAGAAACT TCGGGAAGCT TCTATCCCTC TGGGTATTCT AGTTGTGCGT
    GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGATC GCCTACAGGA GGACCTGCAG
    TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ATGAAACCTC CTTCCCCAGG
    GCGCTCTCAG CTCAGAGACT CTCCCCACGG TGGTGCTTTC TAGATGCAAC TTCTGCTGAT
    CGCTTTTACC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCCCAA
    GATGAGATTT TTATTCTGGA CCCAGATATG GTGTTGTCAC AGCAGGCAGG GACACCTCCA
    GGAATGCCTG ACCTGGTGGT GGAGCAAGCC TCAGGGCTGT CAGAATGGTG GAATCCTGCC
    CTACGGAAGC GTATCCTGAG CGACAGTGGT CTGGGAACGA TTACTCCGCA TTATGAGGAC
    TCCGATTTGA GGGATCTCAG CCACTCACGT GTGCTACAGT CACCAGTCTC TTCAGAAGAT
    CACGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATGGA AAGCTATAAA
    AATGGAGGCA GTCTGCTGAT CCAGGGGCCG GACCACTGCT CCCTCCTTCA CCATGCAGCT
    AAAACTGGCC ACGGGGAGAT CGTAAAATAT ATCCTGGACC ACGGACCTTC TGAGTTATTG
    GATATGGCAG ACAGTGAAAC GGGTGAGACC GCACTGCACA AGGCTGCCTG CCAGCGGAAC
    CGGGCTGTGT GCCAGCTTCT GGTGGATGCG GGAGCATCTC TGAGAAAGAC GGACTCCAAG
    GGTAAGACCC CTCAAGACAG AGCACAGCAA GCTGGAGACC CAGATTTGGC TGCTTACCTA
    GAAAGCTGCC AGAACTATAA GATCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259467.1
    CDS522..2858
    Translation

    Target ORF information:

    RefSeq Version XM_022403759.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403759.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGCTGCATC ACATTGAGGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATCGTCCCG 
    CCCACCTGGA TCATTAAGGT CAAGAAGCCT CAGAACTCAT TGAAGGCTTC AAATCGGAAG
    AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GAACTGAACA GGAAAACAAA
    GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTGTTT
    GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC
    CTGAATCCTC GGCAGGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAGTTA
    TATAGGAAAG TACCAAATCT GCGAATTCTG GCTTGTGGAG GGGATGGAAC GGTGGGCTGG
    ATCCTTTCTA TTCTGGATGA ACTACAGCTG AGCCCTCAGC CTCCCGTCGG GGTCCTTCCT
    CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA
    CCTGTCTCTA AGATCCTTTG CCAAGTAGAA GATGGGACAA TCGTACAGCT GGATCGTTGG
    AACCTCCATG TGGAAAGAAA CCCAGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT
    AAGCTCCCTC TGAATGTTTT CAATAATTAC TTCAGCCTTG GATTTGATGC CCACGTCACA
    CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT
    AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGTTC TAGAGATCTA
    TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CTCCAAAGAT TCAGGAACTG
    AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG
    GGGAACCCAG GAGATCACCA TGATTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA
    GTCATTGGGT TCACTATGGC CTCTTTGGCT GCCCTGCAAG TTGGGGGCCA TGGGGAAAGG
    CTACACCAGT GTCGGGAGGT CATGCTGCTC ACTTACAAAT CCATCCCCAT GCAAGTGGAT
    GGGGAGCCCT GTAGGTTGGC CCCGGCTATG ATTCGGATCT CCTTGAGGAA TCAAGCCAAC
    ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATAT GCACCAGGTG
    TCCGCTGGCG ACCTGCGGAG AGTCTCGGCT CCCCCTGGCT CCTTCACCAC TCCCCAATCT
    GTCCCAGACC GCCTGAGGAT CCGGGTGAAC AAAATCAGTT TACAAGACTA TGAAGGATTC
    CACTATGACA AAGAGAAACT TCGGGAAGCT TCTATCCCTC TGGGTATTCT AGTTGTGCGT
    GGAGACTGTG ATTTGGAGAC TTGCCGTATG TACATAGATC GCCTACAGGA GGACCTGCAG
    TCAGTTTCTT CTGGCTCCCA GAGAGTTCAT TACCAGGACC ATGAAACCTC CTTCCCCAGG
    GCGCTCTCAG CTCAGAGACT CTCCCCACGG TGGTGCTTTC TAGATGCAAC TTCTGCTGAT
    CGCTTTTACC GAATAGACAG ATCTCAGGAA CATTTGCACT TTGTGATGGA GATTTCCCAA
    GATGAGATTT TTATTCTGGA CCCAGATATG GTGTTGTCAC AGCAGGCAGG GACACCTCCA
    GGAATGCCTG ACCTGGTGGT GGAGCAAGCC TCAGGGCTGT CAGAATGGTG GAATCCTGCC
    CTACGGAAGC GTATCCTGAG CGACAGTGGT CTGGGAACGA TTACTCCGCA TTATGAGGAC
    TCCGATTTGA GGGATCTCAG CCACTCACGT GTGCTACAGT CACCAGTCTC TTCAGAAGAT
    CACGCAATTT TGCAGGCAGT AATAGCTGGT GATCTTATGA AGCTAATGGA AAGCTATAAA
    AATGGAGGCA GTCTGCTGAT CCAGGGGCCG GACCACTGCT CCCTCCTTCA CCATGCAGCT
    AAAACTGGCC ACGGGGAGAT CGTAAAATAT ATCCTGGACC ACGGACCTTC TGAGTTATTG
    GATATGGCAG ACAGTGAAAC GGGTGAGACC GCACTGCACA AGGCTGCCTG CCAGCGGAAC
    CGGGCTGTGT GCCAGCTTCT GGTGGATGCG GGAGCATCTC TGAGAAAGAC GGACTCCAAG
    GGTAAGACCC CTCAAGACAG AGCACAGCAA GCTGGAGACC CAGATTTGGC TGCTTACCTA
    GAAAGCTGCC AGAACTATAA GATCATTGGC CATGAGGACC TGGAAACTGC TGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe293862
    Clone ID Related Accession (Same CDS sequence) XM_022403760.1
    Accession Version XM_022403760.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product diacylglycerol kinase iota isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006598.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCTGCATC ACATTGAGGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATCGTCCCG 
    CCCACCTGGA TCATTAAGGT CAAGAAGCCT CAGAACTCAT TGAAGGCTTC AAATCGGAAG
    AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GAACTGAACA GGAAAACAAA
    GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTGTTT
    GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC
    CTGAATCCTC GGCAGGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAGTTA
    TATAGGAAAG TACCAAATCT GCGAATTCTG GCTTGTGGAG GGGATGGAAC GGTGGGCTGG
    ATCCTTTCTA TTCTGGATGA ACTACAGCTG AGCCCTCAGC CTCCCGTCGG GGTCCTTCCT
    CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA
    CCTGTCTCTA AGATCCTTTG CCAAGTAGAA GATGGGACAA TCGTACAGCT GGATCGTTGG
    AACCTCCATG TGGAAAGAAA CCCAGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT
    AAGCTCCCTC TGAATGTTTT CAATAATTAC TTCAGCCTTG GATTTGATGC CCACGTCACA
    CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT
    AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGTTC TAGAGATCTA
    TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CTCCAAAGAT TCAGGAACTG
    AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG
    GGGAACCCAG GAGATCACCA TGATTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA
    GTCATTGGGT TCACTATGGC CTCTTTGGCT GCCCTGCAAG TTGGGGGCCA TGGGGAAAGG
    CTACACCAGT GTCGGGAGGT CATGCTGCTC ACTTACAAAT CCATCCCCAT GCAAGTGGAT
    GGGGAGCCCT GTAGGTTGGC CCCGGCTATG ATTCGGATCT CCTTGAGGAA TCAAGCCAAC
    ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAATCTGTC
    CCAGACCGCC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC
    TATGACAAAG AGAAACTTCG GGAAGCTTCT ATCCCTCTGG GTATTCTAGT TGTGCGTGGA
    GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGATCGCC TACAGGAGGA CCTGCAGTCA
    GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCATG AAACCTCCTT CCCCAGGGCG
    CTCTCAGCTC AGAGACTCTC CCCACGGTGG TGCTTTCTAG ATGCAACTTC TGCTGATCGC
    TTTTACCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCCCAAGAT
    GAGATTTTTA TTCTGGACCC AGATATGGTG TTGTCACAGC AGGCAGGGAC ACCTCCAGGA
    ATGCCTGACC TGGTGGTGGA GCAAGCCTCA GGGCTGTCAG AATGGTGGAA TCCTGCCCTA
    CGGAAGCGTA TCCTGAGCGA CAGTGGTCTG GGAACGATTA CTCCGCATTA TGAGGACTCC
    GATTTGAGGG ATCTCAGCCA CTCACGTGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAC
    GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATGGAAAG CTATAAAAAT
    GGAGGCAGTC TGCTGATCCA GGGGCCGGAC CACTGCTCCC TCCTTCACCA TGCAGCTAAA
    ACTGGCCACG GGGAGATCGT AAAATATATC CTGGACCACG GACCTTCTGA GTTATTGGAT
    ATGGCAGACA GTGAAACGGG TGAGACCGCA CTGCACAAGG CTGCCTGCCA GCGGAACCGG
    GCTGTGTGCC AGCTTCTGGT GGATGCGGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT
    AAGACCCCTC AAGACAGAGC ACAGCAAGCT GGAGACCCAG ATTTGGCTGC TTACCTAGAA
    AGCTGCCAGA ACTATAAGAT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022259468.1
    CDS522..2795
    Translation

    Target ORF information:

    RefSeq Version XM_022403760.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris diacylglycerol kinase iota (DGKI), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022403760.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGCTGCATC ACATTGAGGA ACCCTGCTCC CTGGGGGCTC ATGCTGCTGT TATCGTCCCG 
    CCCACCTGGA TCATTAAGGT CAAGAAGCCT CAGAACTCAT TGAAGGCTTC AAATCGGAAG
    AAGAAGAGAA CAAGCTTTAA AAGAAAAGCC AGTAAAAGAG GAACTGAACA GGAAAACAAA
    GGTCGTCCTT TTGTGATAAA ACCTATCTCT TCTCCTCTCA TGAAACCCTT GCTTGTGTTT
    GTGAACCCCA AGAGTGGAGG CAACCAGGGA ACCAAAGTCC TGCAGATGTT CATGTGGTAC
    CTGAATCCTC GGCAGGTCTT TGATCTTTCT CAGGAAGGGC CAAAAGATGC GCTTGAGTTA
    TATAGGAAAG TACCAAATCT GCGAATTCTG GCTTGTGGAG GGGATGGAAC GGTGGGCTGG
    ATCCTTTCTA TTCTGGATGA ACTACAGCTG AGCCCTCAGC CTCCCGTCGG GGTCCTTCCT
    CTGGGGACTG GGAATGACCT GGCTCGAACT CTCAACTGGG GAGGGGGCTA CACTGATGAA
    CCTGTCTCTA AGATCCTTTG CCAAGTAGAA GATGGGACAA TCGTACAGCT GGATCGTTGG
    AACCTCCATG TGGAAAGAAA CCCAGACTTG CCTCCAGAAG AACTTGAAGA TGGCGTATGT
    AAGCTCCCTC TGAATGTTTT CAATAATTAC TTCAGCCTTG GATTTGATGC CCACGTCACA
    CTGGAGTTCC ATGAATCCAG AGAAGCAAAT CCAGAGAAAT TCAACAGTCG TTTTCGAAAT
    AAAATGTTCT ATGCAGGGGC AGCTTTTTCT GATTTCCTAC AGAGAAGTTC TAGAGATCTA
    TCCAAACATG TTAAAGTTGT TTGTGATGGA ACAGATCTCA CTCCAAAGAT TCAGGAACTG
    AAGTTCCAGT GTATAGTATT TTTAAATATA CCCAGATATT GTGCTGGCAC AATGCCCTGG
    GGGAACCCAG GAGATCACCA TGATTTTGAA CCTCAGCGTC ATGATGATGG TTATATTGAA
    GTCATTGGGT TCACTATGGC CTCTTTGGCT GCCCTGCAAG TTGGGGGCCA TGGGGAAAGG
    CTACACCAGT GTCGGGAGGT CATGCTGCTC ACTTACAAAT CCATCCCCAT GCAAGTGGAT
    GGGGAGCCCT GTAGGTTGGC CCCGGCTATG ATTCGGATCT CCTTGAGGAA TCAAGCCAAC
    ATGGTACAGA AGAGCAAGAG GAGAACATCC ATGCCTTTAC TCAATGATCC CCAATCTGTC
    CCAGACCGCC TGAGGATCCG GGTGAACAAA ATCAGTTTAC AAGACTATGA AGGATTCCAC
    TATGACAAAG AGAAACTTCG GGAAGCTTCT ATCCCTCTGG GTATTCTAGT TGTGCGTGGA
    GACTGTGATT TGGAGACTTG CCGTATGTAC ATAGATCGCC TACAGGAGGA CCTGCAGTCA
    GTTTCTTCTG GCTCCCAGAG AGTTCATTAC CAGGACCATG AAACCTCCTT CCCCAGGGCG
    CTCTCAGCTC AGAGACTCTC CCCACGGTGG TGCTTTCTAG ATGCAACTTC TGCTGATCGC
    TTTTACCGAA TAGACAGATC TCAGGAACAT TTGCACTTTG TGATGGAGAT TTCCCAAGAT
    GAGATTTTTA TTCTGGACCC AGATATGGTG TTGTCACAGC AGGCAGGGAC ACCTCCAGGA
    ATGCCTGACC TGGTGGTGGA GCAAGCCTCA GGGCTGTCAG AATGGTGGAA TCCTGCCCTA
    CGGAAGCGTA TCCTGAGCGA CAGTGGTCTG GGAACGATTA CTCCGCATTA TGAGGACTCC
    GATTTGAGGG ATCTCAGCCA CTCACGTGTG CTACAGTCAC CAGTCTCTTC AGAAGATCAC
    GCAATTTTGC AGGCAGTAAT AGCTGGTGAT CTTATGAAGC TAATGGAAAG CTATAAAAAT
    GGAGGCAGTC TGCTGATCCA GGGGCCGGAC CACTGCTCCC TCCTTCACCA TGCAGCTAAA
    ACTGGCCACG GGGAGATCGT AAAATATATC CTGGACCACG GACCTTCTGA GTTATTGGAT
    ATGGCAGACA GTGAAACGGG TGAGACCGCA CTGCACAAGG CTGCCTGCCA GCGGAACCGG
    GCTGTGTGCC AGCTTCTGGT GGATGCGGGA GCATCTCTGA GAAAGACGGA CTCCAAGGGT
    AAGACCCCTC AAGACAGAGC ACAGCAAGCT GGAGACCCAG ATTTGGCTGC TTACCTAGAA
    AGCTGCCAGA ACTATAAGAT CATTGGCCAT GAGGACCTGG AAACTGCTGT TTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40463
    Clone ID Related Accession (Same CDS sequence) XM_014119884.1
    Accession Version XM_014119884.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2925bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product diacylglycerol kinase iota isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
    ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT
    CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA
    GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT
    GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC
    GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG
    CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA
    AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG
    CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC
    AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC
    ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC
    ATTAAGGTCA AGAAGCCTCA GAACTCATTG AAGGCTTCAA ATCGGAAGAA GAAGAGAACA
    AGCTTTAAAA GAAAAGCCAG TAAAAGAGGA ACTGAACAGG AAAACAAAGG TCGTCCTTTT
    GTGATAAAAC CTATCTCTTC TCCTCTCATG AAACCCTTGC TTGTGTTTGT GAACCCCAAG
    AGTGGAGGCA ACCAGGGAAC CAAAGTCCTG CAGATGTTCA TGTGGTACCT GAATCCTCGG
    CAGGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TTGAGTTATA TAGGAAAGTA
    CCAAATCTGC GAATTCTGGC TTGTGGAGGG GATGGAACGG TGGGCTGGAT CCTTTCTATT
    CTGGATGAAC TACAGCTGAG CCCTCAGCCT CCCGTCGGGG TCCTTCCTCT GGGGACTGGG
    AATGACCTGG CTCGAACTCT CAACTGGGGA GGGGGCTACA CTGATGAACC TGTCTCTAAG
    ATCCTTTGCC AAGTAGAAGA TGGGACAATC GTACAGCTGG ATCGTTGGAA CCTCCATGTG
    GAAAGAAACC CAGACTTGCC TCCAGAAGAA CTTGAAGATG GCGTATGTAA GCTCCCTCTG
    AATGTTTTCA ATAATTACTT CAGCCTTGGA TTTGATGCCC ACGTCACACT GGAGTTCCAT
    GAATCCAGAG AAGCAAATCC AGAGAAATTC AACAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CTTTTTCTGA TTTCCTACAG AGAAGTTCTA GAGATCTATC CAAACATGTT
    AAAGTTGTTT GTGATGGAAC AGATCTCACT CCAAAGATTC AGGAACTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATACC CAGATATTGT GCTGGCACAA TGCCCTGGGG GAACCCAGGA
    GATCACCATG ATTTTGAACC TCAGCGTCAT GATGATGGTT ATATTGAAGT CATTGGGTTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGCCATG GGGAAAGGCT ACACCAGTGT
    CGGGAGGTCA TGCTGCTCAC TTACAAATCC ATCCCCATGC AAGTGGATGG GGAGCCCTGT
    AGGTTGGCCC CGGCTATGAT TCGGATCTCC TTGAGGAATC AAGCCAACAT GGTACAGAAG
    AGCAAGAGGA GAACATCCAT GCCTTTACTC AATGATATGC ACCAGGTGTC CGCTGGCGAC
    CTGCGGAGAG TCTCGGCTCC CCCTGGCTCC TTCACCACTC CCCAATCTGT CCCAGACCGC
    CTGAGGATCC GGGTGAACAA AATCAGTTTA CAAGACTATG AAGGATTCCA CTATGACAAA
    GAGAAACTTC GGGAAGCTTC TATCCCTCTG GGTATTCTAG TTGTGCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGATCGC CTACAGGAGG ACCTGCAGTC AGTTTCTTCT
    GGCTCCCAGA GAGTTCATTA CCAGGACCAT GAAACCTCCT TCCCCAGGGC GCTCTCAGCT
    CAGAGACTCT CCCCACGGTG GTGCTTTCTA GATGCAACTT CTGCTGATCG CTTTTACCGA
    ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCCCAAGA TGAGATTTTT
    ATTCTGGACC CAGATATGGT GTTGTCACAG CAGGCAGGGA CACCTCCAGG AATGCCTGAC
    CTGGTGGTGG AGCAAGCCTC AGGGCTGTCA GAATGGTGGA ATCCTGCCCT ACGGAAGCGT
    ATCCTGAGCG ACAGTGGTCT GGGAACGATT ACTCCGCATT ATGAGGACTC CGATTTGAGG
    GATCTCAGCC ACTCACGTGT GCTACAGTCA CCAGTCTCTT CAGAAGATCA CGCAATTTTG
    CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATGGAAA GCTATAAAAA TGGAGGCAGT
    CTGCTGATCC AGGGGCCGGA CCACTGCTCC CTCCTTCACC ATGCAGCTAA AACTGGCCAC
    GGGGAGATCG TAAAATATAT CCTGGACCAC GGACCTTCTG AGTTATTGGA TATGGCAGAC
    AGTGAAACGG GTGAGACCGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC
    CAGCTTCTGG TGGATGCGGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACCCCT
    CAAGACAGAG CACAGCAAGC TGGAGACCCA GATTTGGCTG CTTACCTAGA AAGCTGCCAG
    AACTATAAGA TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975359.1
    CDS182..3106
    Translation

    Target ORF information:

    RefSeq Version XM_014119884.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119884.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
    ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT
    CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA
    GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT
    GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC
    GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG
    CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA
    AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG
    CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC
    AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC
    ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC
    ATTAAGGTCA AGAAGCCTCA GAACTCATTG AAGGCTTCAA ATCGGAAGAA GAAGAGAACA
    AGCTTTAAAA GAAAAGCCAG TAAAAGAGGA ACTGAACAGG AAAACAAAGG TCGTCCTTTT
    GTGATAAAAC CTATCTCTTC TCCTCTCATG AAACCCTTGC TTGTGTTTGT GAACCCCAAG
    AGTGGAGGCA ACCAGGGAAC CAAAGTCCTG CAGATGTTCA TGTGGTACCT GAATCCTCGG
    CAGGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TTGAGTTATA TAGGAAAGTA
    CCAAATCTGC GAATTCTGGC TTGTGGAGGG GATGGAACGG TGGGCTGGAT CCTTTCTATT
    CTGGATGAAC TACAGCTGAG CCCTCAGCCT CCCGTCGGGG TCCTTCCTCT GGGGACTGGG
    AATGACCTGG CTCGAACTCT CAACTGGGGA GGGGGCTACA CTGATGAACC TGTCTCTAAG
    ATCCTTTGCC AAGTAGAAGA TGGGACAATC GTACAGCTGG ATCGTTGGAA CCTCCATGTG
    GAAAGAAACC CAGACTTGCC TCCAGAAGAA CTTGAAGATG GCGTATGTAA GCTCCCTCTG
    AATGTTTTCA ATAATTACTT CAGCCTTGGA TTTGATGCCC ACGTCACACT GGAGTTCCAT
    GAATCCAGAG AAGCAAATCC AGAGAAATTC AACAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CTTTTTCTGA TTTCCTACAG AGAAGTTCTA GAGATCTATC CAAACATGTT
    AAAGTTGTTT GTGATGGAAC AGATCTCACT CCAAAGATTC AGGAACTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATACC CAGATATTGT GCTGGCACAA TGCCCTGGGG GAACCCAGGA
    GATCACCATG ATTTTGAACC TCAGCGTCAT GATGATGGTT ATATTGAAGT CATTGGGTTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGCCATG GGGAAAGGCT ACACCAGTGT
    CGGGAGGTCA TGCTGCTCAC TTACAAATCC ATCCCCATGC AAGTGGATGG GGAGCCCTGT
    AGGTTGGCCC CGGCTATGAT TCGGATCTCC TTGAGGAATC AAGCCAACAT GGTACAGAAG
    AGCAAGAGGA GAACATCCAT GCCTTTACTC AATGATATGC ACCAGGTGTC CGCTGGCGAC
    CTGCGGAGAG TCTCGGCTCC CCCTGGCTCC TTCACCACTC CCCAATCTGT CCCAGACCGC
    CTGAGGATCC GGGTGAACAA AATCAGTTTA CAAGACTATG AAGGATTCCA CTATGACAAA
    GAGAAACTTC GGGAAGCTTC TATCCCTCTG GGTATTCTAG TTGTGCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGATCGC CTACAGGAGG ACCTGCAGTC AGTTTCTTCT
    GGCTCCCAGA GAGTTCATTA CCAGGACCAT GAAACCTCCT TCCCCAGGGC GCTCTCAGCT
    CAGAGACTCT CCCCACGGTG GTGCTTTCTA GATGCAACTT CTGCTGATCG CTTTTACCGA
    ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCCCAAGA TGAGATTTTT
    ATTCTGGACC CAGATATGGT GTTGTCACAG CAGGCAGGGA CACCTCCAGG AATGCCTGAC
    CTGGTGGTGG AGCAAGCCTC AGGGCTGTCA GAATGGTGGA ATCCTGCCCT ACGGAAGCGT
    ATCCTGAGCG ACAGTGGTCT GGGAACGATT ACTCCGCATT ATGAGGACTC CGATTTGAGG
    GATCTCAGCC ACTCACGTGT GCTACAGTCA CCAGTCTCTT CAGAAGATCA CGCAATTTTG
    CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATGGAAA GCTATAAAAA TGGAGGCAGT
    CTGCTGATCC AGGGGCCGGA CCACTGCTCC CTCCTTCACC ATGCAGCTAA AACTGGCCAC
    GGGGAGATCG TAAAATATAT CCTGGACCAC GGACCTTCTG AGTTATTGGA TATGGCAGAC
    AGTGAAACGG GTGAGACCGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC
    CAGCTTCTGG TGGATGCGGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACCCCT
    CAAGACAGAG CACAGCAAGC TGGAGACCCA GATTTGGCTG CTTACCTAGA AAGCTGCCAG
    AACTATAAGA TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40464
    Clone ID Related Accession (Same CDS sequence) XM_014119885.1
    Accession Version XM_014119885.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2862bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product diacylglycerol kinase iota isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
    ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT
    CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA
    GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT
    GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC
    GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG
    CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA
    AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG
    CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC
    AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC
    ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC
    ATTAAGGTCA AGAAGCCTCA GAACTCATTG AAGGCTTCAA ATCGGAAGAA GAAGAGAACA
    AGCTTTAAAA GAAAAGCCAG TAAAAGAGGA ACTGAACAGG AAAACAAAGG TCGTCCTTTT
    GTGATAAAAC CTATCTCTTC TCCTCTCATG AAACCCTTGC TTGTGTTTGT GAACCCCAAG
    AGTGGAGGCA ACCAGGGAAC CAAAGTCCTG CAGATGTTCA TGTGGTACCT GAATCCTCGG
    CAGGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TTGAGTTATA TAGGAAAGTA
    CCAAATCTGC GAATTCTGGC TTGTGGAGGG GATGGAACGG TGGGCTGGAT CCTTTCTATT
    CTGGATGAAC TACAGCTGAG CCCTCAGCCT CCCGTCGGGG TCCTTCCTCT GGGGACTGGG
    AATGACCTGG CTCGAACTCT CAACTGGGGA GGGGGCTACA CTGATGAACC TGTCTCTAAG
    ATCCTTTGCC AAGTAGAAGA TGGGACAATC GTACAGCTGG ATCGTTGGAA CCTCCATGTG
    GAAAGAAACC CAGACTTGCC TCCAGAAGAA CTTGAAGATG GCGTATGTAA GCTCCCTCTG
    AATGTTTTCA ATAATTACTT CAGCCTTGGA TTTGATGCCC ACGTCACACT GGAGTTCCAT
    GAATCCAGAG AAGCAAATCC AGAGAAATTC AACAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CTTTTTCTGA TTTCCTACAG AGAAGTTCTA GAGATCTATC CAAACATGTT
    AAAGTTGTTT GTGATGGAAC AGATCTCACT CCAAAGATTC AGGAACTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATACC CAGATATTGT GCTGGCACAA TGCCCTGGGG GAACCCAGGA
    GATCACCATG ATTTTGAACC TCAGCGTCAT GATGATGGTT ATATTGAAGT CATTGGGTTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGCCATG GGGAAAGGCT ACACCAGTGT
    CGGGAGGTCA TGCTGCTCAC TTACAAATCC ATCCCCATGC AAGTGGATGG GGAGCCCTGT
    AGGTTGGCCC CGGCTATGAT TCGGATCTCC TTGAGGAATC AAGCCAACAT GGTACAGAAG
    AGCAAGAGGA GAACATCCAT GCCTTTACTC AATGATCCCC AATCTGTCCC AGACCGCCTG
    AGGATCCGGG TGAACAAAAT CAGTTTACAA GACTATGAAG GATTCCACTA TGACAAAGAG
    AAACTTCGGG AAGCTTCTAT CCCTCTGGGT ATTCTAGTTG TGCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGATCGCCTA CAGGAGGACC TGCAGTCAGT TTCTTCTGGC
    TCCCAGAGAG TTCATTACCA GGACCATGAA ACCTCCTTCC CCAGGGCGCT CTCAGCTCAG
    AGACTCTCCC CACGGTGGTG CTTTCTAGAT GCAACTTCTG CTGATCGCTT TTACCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CCCAAGATGA GATTTTTATT
    CTGGACCCAG ATATGGTGTT GTCACAGCAG GCAGGGACAC CTCCAGGAAT GCCTGACCTG
    GTGGTGGAGC AAGCCTCAGG GCTGTCAGAA TGGTGGAATC CTGCCCTACG GAAGCGTATC
    CTGAGCGACA GTGGTCTGGG AACGATTACT CCGCATTATG AGGACTCCGA TTTGAGGGAT
    CTCAGCCACT CACGTGTGCT ACAGTCACCA GTCTCTTCAG AAGATCACGC AATTTTGCAG
    GCAGTAATAG CTGGTGATCT TATGAAGCTA ATGGAAAGCT ATAAAAATGG AGGCAGTCTG
    CTGATCCAGG GGCCGGACCA CTGCTCCCTC CTTCACCATG CAGCTAAAAC TGGCCACGGG
    GAGATCGTAA AATATATCCT GGACCACGGA CCTTCTGAGT TATTGGATAT GGCAGACAGT
    GAAACGGGTG AGACCGCACT GCACAAGGCT GCCTGCCAGC GGAACCGGGC TGTGTGCCAG
    CTTCTGGTGG ATGCGGGAGC ATCTCTGAGA AAGACGGACT CCAAGGGTAA GACCCCTCAA
    GACAGAGCAC AGCAAGCTGG AGACCCAGAT TTGGCTGCTT ACCTAGAAAG CTGCCAGAAC
    TATAAGATCA TTGGCCATGA GGACCTGGAA ACTGCTGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975360.1
    CDS179..3040
    Translation

    Target ORF information:

    RefSeq Version XM_014119885.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119885.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
    ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT
    CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA
    GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT
    GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC
    GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG
    CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA
    AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG
    CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC
    AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC
    ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC
    ATTAAGGTCA AGAAGCCTCA GAACTCATTG AAGGCTTCAA ATCGGAAGAA GAAGAGAACA
    AGCTTTAAAA GAAAAGCCAG TAAAAGAGGA ACTGAACAGG AAAACAAAGG TCGTCCTTTT
    GTGATAAAAC CTATCTCTTC TCCTCTCATG AAACCCTTGC TTGTGTTTGT GAACCCCAAG
    AGTGGAGGCA ACCAGGGAAC CAAAGTCCTG CAGATGTTCA TGTGGTACCT GAATCCTCGG
    CAGGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TTGAGTTATA TAGGAAAGTA
    CCAAATCTGC GAATTCTGGC TTGTGGAGGG GATGGAACGG TGGGCTGGAT CCTTTCTATT
    CTGGATGAAC TACAGCTGAG CCCTCAGCCT CCCGTCGGGG TCCTTCCTCT GGGGACTGGG
    AATGACCTGG CTCGAACTCT CAACTGGGGA GGGGGCTACA CTGATGAACC TGTCTCTAAG
    ATCCTTTGCC AAGTAGAAGA TGGGACAATC GTACAGCTGG ATCGTTGGAA CCTCCATGTG
    GAAAGAAACC CAGACTTGCC TCCAGAAGAA CTTGAAGATG GCGTATGTAA GCTCCCTCTG
    AATGTTTTCA ATAATTACTT CAGCCTTGGA TTTGATGCCC ACGTCACACT GGAGTTCCAT
    GAATCCAGAG AAGCAAATCC AGAGAAATTC AACAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CTTTTTCTGA TTTCCTACAG AGAAGTTCTA GAGATCTATC CAAACATGTT
    AAAGTTGTTT GTGATGGAAC AGATCTCACT CCAAAGATTC AGGAACTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATACC CAGATATTGT GCTGGCACAA TGCCCTGGGG GAACCCAGGA
    GATCACCATG ATTTTGAACC TCAGCGTCAT GATGATGGTT ATATTGAAGT CATTGGGTTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGCCATG GGGAAAGGCT ACACCAGTGT
    CGGGAGGTCA TGCTGCTCAC TTACAAATCC ATCCCCATGC AAGTGGATGG GGAGCCCTGT
    AGGTTGGCCC CGGCTATGAT TCGGATCTCC TTGAGGAATC AAGCCAACAT GGTACAGAAG
    AGCAAGAGGA GAACATCCAT GCCTTTACTC AATGATCCCC AATCTGTCCC AGACCGCCTG
    AGGATCCGGG TGAACAAAAT CAGTTTACAA GACTATGAAG GATTCCACTA TGACAAAGAG
    AAACTTCGGG AAGCTTCTAT CCCTCTGGGT ATTCTAGTTG TGCGTGGAGA CTGTGATTTG
    GAGACTTGCC GTATGTACAT AGATCGCCTA CAGGAGGACC TGCAGTCAGT TTCTTCTGGC
    TCCCAGAGAG TTCATTACCA GGACCATGAA ACCTCCTTCC CCAGGGCGCT CTCAGCTCAG
    AGACTCTCCC CACGGTGGTG CTTTCTAGAT GCAACTTCTG CTGATCGCTT TTACCGAATA
    GACAGATCTC AGGAACATTT GCACTTTGTG ATGGAGATTT CCCAAGATGA GATTTTTATT
    CTGGACCCAG ATATGGTGTT GTCACAGCAG GCAGGGACAC CTCCAGGAAT GCCTGACCTG
    GTGGTGGAGC AAGCCTCAGG GCTGTCAGAA TGGTGGAATC CTGCCCTACG GAAGCGTATC
    CTGAGCGACA GTGGTCTGGG AACGATTACT CCGCATTATG AGGACTCCGA TTTGAGGGAT
    CTCAGCCACT CACGTGTGCT ACAGTCACCA GTCTCTTCAG AAGATCACGC AATTTTGCAG
    GCAGTAATAG CTGGTGATCT TATGAAGCTA ATGGAAAGCT ATAAAAATGG AGGCAGTCTG
    CTGATCCAGG GGCCGGACCA CTGCTCCCTC CTTCACCATG CAGCTAAAAC TGGCCACGGG
    GAGATCGTAA AATATATCCT GGACCACGGA CCTTCTGAGT TATTGGATAT GGCAGACAGT
    GAAACGGGTG AGACCGCACT GCACAAGGCT GCCTGCCAGC GGAACCGGGC TGTGTGCCAG
    CTTCTGGTGG ATGCGGGAGC ATCTCTGAGA AAGACGGACT CCAAGGGTAA GACCCCTCAA
    GACAGAGCAC AGCAAGCTGG AGACCCAGAT TTGGCTGCTT ACCTAGAAAG CTGCCAGAAC
    TATAAGATCA TTGGCCATGA GGACCTGGAA ACTGCTGTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40461
    Clone ID Related Accession (Same CDS sequence) XM_014119882.1
    Accession Version XM_014119882.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3048bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product diacylglycerol kinase iota isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
    ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT
    CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA
    GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT
    GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC
    GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG
    CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA
    AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG
    CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC
    AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC
    ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC
    ATTAAGGTCA AGAAGCCTCA GGTGATCTCC ATACCAGGAG GTGAACTTGA ACTTGGTTGG
    GCGGGAGACG GACCTGGTTG GGCGGGAGAC TGGAGCCCCT CAGAGAATTT GGGATTCTTC
    CATTTTTACT GGGAGGGTTG GCAGAACTCA TTGAAGGCTT CAAATCGGAA GAAGAAGAGA
    ACAAGCTTTA AAAGAAAAGC CAGTAAAAGA GGAACTGAAC AGGAAAACAA AGGTCGTCCT
    TTTGTGATAA AACCTATCTC TTCTCCTCTC ATGAAACCCT TGCTTGTGTT TGTGAACCCC
    AAGAGTGGAG GCAACCAGGG AACCAAAGTC CTGCAGATGT TCATGTGGTA CCTGAATCCT
    CGGCAGGTCT TTGATCTTTC TCAGGAAGGG CCAAAAGATG CGCTTGAGTT ATATAGGAAA
    GTACCAAATC TGCGAATTCT GGCTTGTGGA GGGGATGGAA CGGTGGGCTG GATCCTTTCT
    ATTCTGGATG AACTACAGCT GAGCCCTCAG CCTCCCGTCG GGGTCCTTCC TCTGGGGACT
    GGGAATGACC TGGCTCGAAC TCTCAACTGG GGAGGGGGCT ACACTGATGA ACCTGTCTCT
    AAGATCCTTT GCCAAGTAGA AGATGGGACA ATCGTACAGC TGGATCGTTG GAACCTCCAT
    GTGGAAAGAA ACCCAGACTT GCCTCCAGAA GAACTTGAAG ATGGCGTATG TAAGCTCCCT
    CTGAATGTTT TCAATAATTA CTTCAGCCTT GGATTTGATG CCCACGTCAC ACTGGAGTTC
    CATGAATCCA GAGAAGCAAA TCCAGAGAAA TTCAACAGTC GTTTTCGAAA TAAAATGTTC
    TATGCAGGGG CAGCTTTTTC TGATTTCCTA CAGAGAAGTT CTAGAGATCT ATCCAAACAT
    GTTAAAGTTG TTTGTGATGG AACAGATCTC ACTCCAAAGA TTCAGGAACT GAAGTTCCAG
    TGTATAGTAT TTTTAAATAT ACCCAGATAT TGTGCTGGCA CAATGCCCTG GGGGAACCCA
    GGAGATCACC ATGATTTTGA ACCTCAGCGT CATGATGATG GTTATATTGA AGTCATTGGG
    TTCACTATGG CCTCTTTGGC TGCCCTGCAA GTTGGGGGCC ATGGGGAAAG GCTACACCAG
    TGTCGGGAGG TCATGCTGCT CACTTACAAA TCCATCCCCA TGCAAGTGGA TGGGGAGCCC
    TGTAGGTTGG CCCCGGCTAT GATTCGGATC TCCTTGAGGA ATCAAGCCAA CATGGTACAG
    AAGAGCAAGA GGAGAACATC CATGCCTTTA CTCAATGATA TGCACCAGGT GTCCGCTGGC
    GACCTGCGGA GAGTCTCGGC TCCCCCTGGC TCCTTCACCA CTCCCCAATC TGTCCCAGAC
    CGCCTGAGGA TCCGGGTGAA CAAAATCAGT TTACAAGACT ATGAAGGATT CCACTATGAC
    AAAGAGAAAC TTCGGGAAGC TTCTATCCCT CTGGGTATTC TAGTTGTGCG TGGAGACTGT
    GATTTGGAGA CTTGCCGTAT GTACATAGAT CGCCTACAGG AGGACCTGCA GTCAGTTTCT
    TCTGGCTCCC AGAGAGTTCA TTACCAGGAC CATGAAACCT CCTTCCCCAG GGCGCTCTCA
    GCTCAGAGAC TCTCCCCACG GTGGTGCTTT CTAGATGCAA CTTCTGCTGA TCGCTTTTAC
    CGAATAGACA GATCTCAGGA ACATTTGCAC TTTGTGATGG AGATTTCCCA AGATGAGATT
    TTTATTCTGG ACCCAGATAT GGTGTTGTCA CAGCAGGCAG GGACACCTCC AGGAATGCCT
    GACCTGGTGG TGGAGCAAGC CTCAGGGCTG TCAGAATGGT GGAATCCTGC CCTACGGAAG
    CGTATCCTGA GCGACAGTGG TCTGGGAACG ATTACTCCGC ATTATGAGGA CTCCGATTTG
    AGGGATCTCA GCCACTCACG TGTGCTACAG TCACCAGTCT CTTCAGAAGA TCACGCAATT
    TTGCAGGCAG TAATAGCTGG TGATCTTATG AAGCTAATGG AAAGCTATAA AAATGGAGGC
    AGTCTGCTGA TCCAGGGGCC GGACCACTGC TCCCTCCTTC ACCATGCAGC TAAAACTGGC
    CACGGGGAGA TCGTAAAATA TATCCTGGAC CACGGACCTT CTGAGTTATT GGATATGGCA
    GACAGTGAAA CGGGTGAGAC CGCACTGCAC AAGGCTGCCT GCCAGCGGAA CCGGGCTGTG
    TGCCAGCTTC TGGTGGATGC GGGAGCATCT CTGAGAAAGA CGGACTCCAA GGGTAAGACC
    CCTCAAGACA GAGCACAGCA AGCTGGAGAC CCAGATTTGG CTGCTTACCT AGAAAGCTGC
    CAGAACTATA AGATCATTGG CCATGAGGAC CTGGAAACTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975357.1
    CDS199..3246
    Translation

    Target ORF information:

    RefSeq Version XM_014119882.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119882.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
    ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT
    CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA
    GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT
    GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC
    GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG
    CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA
    AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG
    CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC
    AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC
    ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC
    ATTAAGGTCA AGAAGCCTCA GGTGATCTCC ATACCAGGAG GTGAACTTGA ACTTGGTTGG
    GCGGGAGACG GACCTGGTTG GGCGGGAGAC TGGAGCCCCT CAGAGAATTT GGGATTCTTC
    CATTTTTACT GGGAGGGTTG GCAGAACTCA TTGAAGGCTT CAAATCGGAA GAAGAAGAGA
    ACAAGCTTTA AAAGAAAAGC CAGTAAAAGA GGAACTGAAC AGGAAAACAA AGGTCGTCCT
    TTTGTGATAA AACCTATCTC TTCTCCTCTC ATGAAACCCT TGCTTGTGTT TGTGAACCCC
    AAGAGTGGAG GCAACCAGGG AACCAAAGTC CTGCAGATGT TCATGTGGTA CCTGAATCCT
    CGGCAGGTCT TTGATCTTTC TCAGGAAGGG CCAAAAGATG CGCTTGAGTT ATATAGGAAA
    GTACCAAATC TGCGAATTCT GGCTTGTGGA GGGGATGGAA CGGTGGGCTG GATCCTTTCT
    ATTCTGGATG AACTACAGCT GAGCCCTCAG CCTCCCGTCG GGGTCCTTCC TCTGGGGACT
    GGGAATGACC TGGCTCGAAC TCTCAACTGG GGAGGGGGCT ACACTGATGA ACCTGTCTCT
    AAGATCCTTT GCCAAGTAGA AGATGGGACA ATCGTACAGC TGGATCGTTG GAACCTCCAT
    GTGGAAAGAA ACCCAGACTT GCCTCCAGAA GAACTTGAAG ATGGCGTATG TAAGCTCCCT
    CTGAATGTTT TCAATAATTA CTTCAGCCTT GGATTTGATG CCCACGTCAC ACTGGAGTTC
    CATGAATCCA GAGAAGCAAA TCCAGAGAAA TTCAACAGTC GTTTTCGAAA TAAAATGTTC
    TATGCAGGGG CAGCTTTTTC TGATTTCCTA CAGAGAAGTT CTAGAGATCT ATCCAAACAT
    GTTAAAGTTG TTTGTGATGG AACAGATCTC ACTCCAAAGA TTCAGGAACT GAAGTTCCAG
    TGTATAGTAT TTTTAAATAT ACCCAGATAT TGTGCTGGCA CAATGCCCTG GGGGAACCCA
    GGAGATCACC ATGATTTTGA ACCTCAGCGT CATGATGATG GTTATATTGA AGTCATTGGG
    TTCACTATGG CCTCTTTGGC TGCCCTGCAA GTTGGGGGCC ATGGGGAAAG GCTACACCAG
    TGTCGGGAGG TCATGCTGCT CACTTACAAA TCCATCCCCA TGCAAGTGGA TGGGGAGCCC
    TGTAGGTTGG CCCCGGCTAT GATTCGGATC TCCTTGAGGA ATCAAGCCAA CATGGTACAG
    AAGAGCAAGA GGAGAACATC CATGCCTTTA CTCAATGATA TGCACCAGGT GTCCGCTGGC
    GACCTGCGGA GAGTCTCGGC TCCCCCTGGC TCCTTCACCA CTCCCCAATC TGTCCCAGAC
    CGCCTGAGGA TCCGGGTGAA CAAAATCAGT TTACAAGACT ATGAAGGATT CCACTATGAC
    AAAGAGAAAC TTCGGGAAGC TTCTATCCCT CTGGGTATTC TAGTTGTGCG TGGAGACTGT
    GATTTGGAGA CTTGCCGTAT GTACATAGAT CGCCTACAGG AGGACCTGCA GTCAGTTTCT
    TCTGGCTCCC AGAGAGTTCA TTACCAGGAC CATGAAACCT CCTTCCCCAG GGCGCTCTCA
    GCTCAGAGAC TCTCCCCACG GTGGTGCTTT CTAGATGCAA CTTCTGCTGA TCGCTTTTAC
    CGAATAGACA GATCTCAGGA ACATTTGCAC TTTGTGATGG AGATTTCCCA AGATGAGATT
    TTTATTCTGG ACCCAGATAT GGTGTTGTCA CAGCAGGCAG GGACACCTCC AGGAATGCCT
    GACCTGGTGG TGGAGCAAGC CTCAGGGCTG TCAGAATGGT GGAATCCTGC CCTACGGAAG
    CGTATCCTGA GCGACAGTGG TCTGGGAACG ATTACTCCGC ATTATGAGGA CTCCGATTTG
    AGGGATCTCA GCCACTCACG TGTGCTACAG TCACCAGTCT CTTCAGAAGA TCACGCAATT
    TTGCAGGCAG TAATAGCTGG TGATCTTATG AAGCTAATGG AAAGCTATAA AAATGGAGGC
    AGTCTGCTGA TCCAGGGGCC GGACCACTGC TCCCTCCTTC ACCATGCAGC TAAAACTGGC
    CACGGGGAGA TCGTAAAATA TATCCTGGAC CACGGACCTT CTGAGTTATT GGATATGGCA
    GACAGTGAAA CGGGTGAGAC CGCACTGCAC AAGGCTGCCT GCCAGCGGAA CCGGGCTGTG
    TGCCAGCTTC TGGTGGATGC GGGAGCATCT CTGAGAAAGA CGGACTCCAA GGGTAAGACC
    CCTCAAGACA GAGCACAGCA AGCTGGAGAC CCAGATTTGG CTGCTTACCT AGAAAGCTGC
    CAGAACTATA AGATCATTGG CCATGAGGAC CTGGAAACTG CTGTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe40462
    Clone ID Related Accession (Same CDS sequence) XM_014119883.1
    Accession Version XM_014119883.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3045bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product diacylglycerol kinase iota isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726086.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
    ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT
    CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA
    GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT
    GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC
    GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG
    CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA
    AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG
    CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC
    AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC
    ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC
    ATTAAGGTCA AGAAGCCTCA GGTGATCTCC ATACCAGGAG GTGAACTTGA ACTTGGTTGG
    GCGGGAGACG GACCTGGTTG GGCGGGAGAC TGGAGCCCCT CAGAGAATTT GGGATTCTTC
    CATTTTTACT GGGAGGGTTG GCAGAACTCA TTGAAGGCTT CAAATCGGAA GAAGAAGAGA
    ACAAGCTTTA AAAGAAAAGC CAGTAAAAGA GGAACTGAAG AAAACAAAGG TCGTCCTTTT
    GTGATAAAAC CTATCTCTTC TCCTCTCATG AAACCCTTGC TTGTGTTTGT GAACCCCAAG
    AGTGGAGGCA ACCAGGGAAC CAAAGTCCTG CAGATGTTCA TGTGGTACCT GAATCCTCGG
    CAGGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TTGAGTTATA TAGGAAAGTA
    CCAAATCTGC GAATTCTGGC TTGTGGAGGG GATGGAACGG TGGGCTGGAT CCTTTCTATT
    CTGGATGAAC TACAGCTGAG CCCTCAGCCT CCCGTCGGGG TCCTTCCTCT GGGGACTGGG
    AATGACCTGG CTCGAACTCT CAACTGGGGA GGGGGCTACA CTGATGAACC TGTCTCTAAG
    ATCCTTTGCC AAGTAGAAGA TGGGACAATC GTACAGCTGG ATCGTTGGAA CCTCCATGTG
    GAAAGAAACC CAGACTTGCC TCCAGAAGAA CTTGAAGATG GCGTATGTAA GCTCCCTCTG
    AATGTTTTCA ATAATTACTT CAGCCTTGGA TTTGATGCCC ACGTCACACT GGAGTTCCAT
    GAATCCAGAG AAGCAAATCC AGAGAAATTC AACAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CTTTTTCTGA TTTCCTACAG AGAAGTTCTA GAGATCTATC CAAACATGTT
    AAAGTTGTTT GTGATGGAAC AGATCTCACT CCAAAGATTC AGGAACTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATACC CAGATATTGT GCTGGCACAA TGCCCTGGGG GAACCCAGGA
    GATCACCATG ATTTTGAACC TCAGCGTCAT GATGATGGTT ATATTGAAGT CATTGGGTTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGCCATG GGGAAAGGCT ACACCAGTGT
    CGGGAGGTCA TGCTGCTCAC TTACAAATCC ATCCCCATGC AAGTGGATGG GGAGCCCTGT
    AGGTTGGCCC CGGCTATGAT TCGGATCTCC TTGAGGAATC AAGCCAACAT GGTACAGAAG
    AGCAAGAGGA GAACATCCAT GCCTTTACTC AATGATATGC ACCAGGTGTC CGCTGGCGAC
    CTGCGGAGAG TCTCGGCTCC CCCTGGCTCC TTCACCACTC CCCAATCTGT CCCAGACCGC
    CTGAGGATCC GGGTGAACAA AATCAGTTTA CAAGACTATG AAGGATTCCA CTATGACAAA
    GAGAAACTTC GGGAAGCTTC TATCCCTCTG GGTATTCTAG TTGTGCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGATCGC CTACAGGAGG ACCTGCAGTC AGTTTCTTCT
    GGCTCCCAGA GAGTTCATTA CCAGGACCAT GAAACCTCCT TCCCCAGGGC GCTCTCAGCT
    CAGAGACTCT CCCCACGGTG GTGCTTTCTA GATGCAACTT CTGCTGATCG CTTTTACCGA
    ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCCCAAGA TGAGATTTTT
    ATTCTGGACC CAGATATGGT GTTGTCACAG CAGGCAGGGA CACCTCCAGG AATGCCTGAC
    CTGGTGGTGG AGCAAGCCTC AGGGCTGTCA GAATGGTGGA ATCCTGCCCT ACGGAAGCGT
    ATCCTGAGCG ACAGTGGTCT GGGAACGATT ACTCCGCATT ATGAGGACTC CGATTTGAGG
    GATCTCAGCC ACTCACGTGT GCTACAGTCA CCAGTCTCTT CAGAAGATCA CGCAATTTTG
    CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATGGAAA GCTATAAAAA TGGAGGCAGT
    CTGCTGATCC AGGGGCCGGA CCACTGCTCC CTCCTTCACC ATGCAGCTAA AACTGGCCAC
    GGGGAGATCG TAAAATATAT CCTGGACCAC GGACCTTCTG AGTTATTGGA TATGGCAGAC
    AGTGAAACGG GTGAGACCGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC
    CAGCTTCTGG TGGATGCGGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACCCCT
    CAAGACAGAG CACAGCAAGC TGGAGACCCA GATTTGGCTG CTTACCTAGA AAGCTGCCAG
    AACTATAAGA TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013975358.1
    CDS322..3366
    Translation

    Target ORF information:

    RefSeq Version XM_014119883.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris diacylglycerol kinase, iota (DGKI), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014119883.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGAGTCCAG GATGGGTGGA TCTCTTTTGC AGAAGCTGCA GGAACCAAGA CAGGTGCACA 
    ACCCCAGTAC CAGAGGCCTC AGATGGCTGG AAAGCAATCT CCCGGGCAGG CCTGCAGCAT
    CTGGCTCCTG CACATCCCCT CAGCCTTCCT GTGGCAAATG GTCCAGCAAA GGAGCCCAGA
    GCGACTCTGG ACTGGAGTGA GAATGCTGTG AACGGAGAGC ACCTGTGGCT GGAGACCAAT
    GTCTCAGGAG ACTTATGCTA CCTGGGAGAA GAGAACTGCC AAGTCAGATT TGCAAAATCC
    GCCCTGAGGA GGAAGTGTGC GGTCTGTAAA ATCGTCGTCC ACGCTGCCTG CATTGAACAG
    CTAGAGAAGA TTAATTTCAG ATGTAAGCCA ACATTTCGAG AAGGGGGCTC AAGATCACCA
    AGAGAAAACT TTGTGCGTCA TCACTGGGTG CACAGGCGTC GGCAGGAGGG AAAATGTAAG
    CAGTGTGGTA AGGGCTTCCA GCAAAAATTC TCCTTCCACA GTAAAGAGAT TGTGGCTATC
    AGCTGTTCCT GGTGCAAGCA GGCGTTTCAC AATAAGGTGA CCTGCTTCAT GCTGCATCAC
    ATTGAGGAAC CCTGCTCCCT GGGGGCTCAT GCTGCTGTTA TCGTCCCGCC CACCTGGATC
    ATTAAGGTCA AGAAGCCTCA GGTGATCTCC ATACCAGGAG GTGAACTTGA ACTTGGTTGG
    GCGGGAGACG GACCTGGTTG GGCGGGAGAC TGGAGCCCCT CAGAGAATTT GGGATTCTTC
    CATTTTTACT GGGAGGGTTG GCAGAACTCA TTGAAGGCTT CAAATCGGAA GAAGAAGAGA
    ACAAGCTTTA AAAGAAAAGC CAGTAAAAGA GGAACTGAAG AAAACAAAGG TCGTCCTTTT
    GTGATAAAAC CTATCTCTTC TCCTCTCATG AAACCCTTGC TTGTGTTTGT GAACCCCAAG
    AGTGGAGGCA ACCAGGGAAC CAAAGTCCTG CAGATGTTCA TGTGGTACCT GAATCCTCGG
    CAGGTCTTTG ATCTTTCTCA GGAAGGGCCA AAAGATGCGC TTGAGTTATA TAGGAAAGTA
    CCAAATCTGC GAATTCTGGC TTGTGGAGGG GATGGAACGG TGGGCTGGAT CCTTTCTATT
    CTGGATGAAC TACAGCTGAG CCCTCAGCCT CCCGTCGGGG TCCTTCCTCT GGGGACTGGG
    AATGACCTGG CTCGAACTCT CAACTGGGGA GGGGGCTACA CTGATGAACC TGTCTCTAAG
    ATCCTTTGCC AAGTAGAAGA TGGGACAATC GTACAGCTGG ATCGTTGGAA CCTCCATGTG
    GAAAGAAACC CAGACTTGCC TCCAGAAGAA CTTGAAGATG GCGTATGTAA GCTCCCTCTG
    AATGTTTTCA ATAATTACTT CAGCCTTGGA TTTGATGCCC ACGTCACACT GGAGTTCCAT
    GAATCCAGAG AAGCAAATCC AGAGAAATTC AACAGTCGTT TTCGAAATAA AATGTTCTAT
    GCAGGGGCAG CTTTTTCTGA TTTCCTACAG AGAAGTTCTA GAGATCTATC CAAACATGTT
    AAAGTTGTTT GTGATGGAAC AGATCTCACT CCAAAGATTC AGGAACTGAA GTTCCAGTGT
    ATAGTATTTT TAAATATACC CAGATATTGT GCTGGCACAA TGCCCTGGGG GAACCCAGGA
    GATCACCATG ATTTTGAACC TCAGCGTCAT GATGATGGTT ATATTGAAGT CATTGGGTTC
    ACTATGGCCT CTTTGGCTGC CCTGCAAGTT GGGGGCCATG GGGAAAGGCT ACACCAGTGT
    CGGGAGGTCA TGCTGCTCAC TTACAAATCC ATCCCCATGC AAGTGGATGG GGAGCCCTGT
    AGGTTGGCCC CGGCTATGAT TCGGATCTCC TTGAGGAATC AAGCCAACAT GGTACAGAAG
    AGCAAGAGGA GAACATCCAT GCCTTTACTC AATGATATGC ACCAGGTGTC CGCTGGCGAC
    CTGCGGAGAG TCTCGGCTCC CCCTGGCTCC TTCACCACTC CCCAATCTGT CCCAGACCGC
    CTGAGGATCC GGGTGAACAA AATCAGTTTA CAAGACTATG AAGGATTCCA CTATGACAAA
    GAGAAACTTC GGGAAGCTTC TATCCCTCTG GGTATTCTAG TTGTGCGTGG AGACTGTGAT
    TTGGAGACTT GCCGTATGTA CATAGATCGC CTACAGGAGG ACCTGCAGTC AGTTTCTTCT
    GGCTCCCAGA GAGTTCATTA CCAGGACCAT GAAACCTCCT TCCCCAGGGC GCTCTCAGCT
    CAGAGACTCT CCCCACGGTG GTGCTTTCTA GATGCAACTT CTGCTGATCG CTTTTACCGA
    ATAGACAGAT CTCAGGAACA TTTGCACTTT GTGATGGAGA TTTCCCAAGA TGAGATTTTT
    ATTCTGGACC CAGATATGGT GTTGTCACAG CAGGCAGGGA CACCTCCAGG AATGCCTGAC
    CTGGTGGTGG AGCAAGCCTC AGGGCTGTCA GAATGGTGGA ATCCTGCCCT ACGGAAGCGT
    ATCCTGAGCG ACAGTGGTCT GGGAACGATT ACTCCGCATT ATGAGGACTC CGATTTGAGG
    GATCTCAGCC ACTCACGTGT GCTACAGTCA CCAGTCTCTT CAGAAGATCA CGCAATTTTG
    CAGGCAGTAA TAGCTGGTGA TCTTATGAAG CTAATGGAAA GCTATAAAAA TGGAGGCAGT
    CTGCTGATCC AGGGGCCGGA CCACTGCTCC CTCCTTCACC ATGCAGCTAA AACTGGCCAC
    GGGGAGATCG TAAAATATAT CCTGGACCAC GGACCTTCTG AGTTATTGGA TATGGCAGAC
    AGTGAAACGG GTGAGACCGC ACTGCACAAG GCTGCCTGCC AGCGGAACCG GGCTGTGTGC
    CAGCTTCTGG TGGATGCGGG AGCATCTCTG AGAAAGACGG ACTCCAAGGG TAAGACCCCT
    CAAGACAGAG CACAGCAAGC TGGAGACCCA GATTTGGCTG CTTACCTAGA AAGCTGCCAG
    AACTATAAGA TCATTGGCCA TGAGGACCTG GAAACTGCTG TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.