HUWE1 cDNA ORF clone, Canis lupus familiaris(dog)

The following HUWE1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HUWE1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe32412 XM_005641381.3
Latest version!
Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45532 XM_014111522.2
Latest version!
Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45532 XM_014111523.2
Latest version!
Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45532 XM_014111521.2
Latest version!
Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45532 XM_014111524.2
Latest version!
Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45532 XM_014111525.1
Latest version!
Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45533 XM_014111526.2
Latest version!
Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45534 XM_014111527.2
Latest version!
Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45535 XM_014111528.2
Latest version!
Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
OCe32412 XM_005641381.2 Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45534 XM_014111527.1 Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45532 XM_014111522.1 Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45532 XM_014111521.1 Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45533 XM_014111526.1 Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45535 XM_014111528.1 Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45532 XM_014111523.1 Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
OCe45532 XM_014111524.1 Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe32412
    Clone ID Related Accession (Same CDS sequence) XM_005641381.2 , XM_005641381.3
    Accession Version XM_005641381.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13263bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005641381.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    2641
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    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAC CTCTCTTTCC
    CCAGAGAGGG CTGAAGATTC TGATGCGCTG ACAGCTGTCA GCAGTCAACT GGAAGGCTCT
    CCCATGGACA CCAGTAGCCT GGCTTCCTGT ACCCTAGAGG AGGCTGTGGG TGACACCTCA
    GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGCAGCTCCA CTCCTGGGGA TGCCCCAACT
    GTTGTGACAG AAGTGCAAAG CAGGGGTGAT GGGTCAGGTG AACCTACCCA GCCCCCTGAA
    GACAGCTCTC CCCCTGCATC CTCTGAGAGT TCTTCCACCA GAGATTCTGC TGTGGCCATT
    TCTGGAGCAG ATTCCCGAGG AATCCTAGAA GAGCCACTGC CTTCAACAAG CAGTGAGGAA
    GAAGATCCCC TTGCAGGTAT CAGTCTACCA GAAGGTGTGG ACCCCTCTTT CCTGGCTGCC
    CTGCCTGATG ACATCCGCCG GGAAGTCCTA CAGAACCAAC TGGGCATCCG TCCACCAACT
    CGGACTGCCC CTTCGGCAAA TAGCTCAGCT CCTGCAGTGG TGGGGAATCC TGGTGTGACC
    GAAGTGAGCC CTGAGTTCCT GGCGGCCCTG CCTCCAGCCA TTCAGGAGGA AGTGCTGGCA
    CAGCAGAGAG CTGAGCAACA ACGACGGGAA CTGGCACAGA ATGCGAGCTC AGACACTCCC
    ATGGACCCTG TGACCTTCAT CCAGACCCTG CCATCAGACT TGCGCCGTAG TGTCCTGGAG
    GACATGGAGG ACAGTGTTTT GGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCC
    CTGAGGCGAG AGCAGGAAGC CCGGCAGCGA CAGCTCATGC ATGAGCGGCT CTTTGGCCAC
    AGCAGCACCT CTGCGCTCTC TGCCATCCTC CGAAGCCCGG CTTTCACCAG TCGCCTCAGT
    GGCAACCGAG GAGTCCAATA CACTCGTCTT GCTGTGCAAA GAGGTGGCAC CTTCCAGATG
    GGGGGTAGCA GCAGCCATAA CAGGCCTTCT GGCAGTAATG TGGATACTCT TCTCCGCCTC
    CGAGGACGGC TCCTCCTGGA CCATGAAGCC CTATCCTGTC TCCTGGTCCT ACTTTTCGTG
    GATGAGCCAA AGCTTAATAC TAGTCGTCTA CACCGAGTAC TGAGAAATCT CTGCTACCAT
    GCCCAGACCC GCCACTGGGT CATCCGCAGT CTGCTCTCCA TTTTGCAGCG CAGCAGTGAA
    AGTGAGCTAT GCATTGAAAC ACCCAAGCTC TCCTCAAGTG AGGACAAGGG CAAAAAGTCA
    AGCAAAAGCT GTGGGTCAAG CAGCCATGAG AACCGGCCCC TGGACCTCCT ACACAAGATG
    GAGTCTAAGA GCTCCAACCA GCTTTCCTGG CTCTCAGTAT CCATGGATGC AGCCCTCGGC
    TGCAGGACTA ATATATTCCA GATTCAACGT TCAGGAGGTC GCAAACATAC CGAGAAGCAT
    GCAAGCAGTG GCTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT CTGCAGACAT
    GTTCTAGACA CACTCATTCA ATTGGCCAAG GTATTTCCCA GCCATTTTAC ACAGCAGCGG
    ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAGAGGGGCA GTAAGCAGGC CTGCAGCCCA
    TGCTCTTCAC AGTCCACCAG CAGTGGGATT TGCACAGACT TCTGGGACTT ACTGGTTAAA
    CTGGACAACA TGAATGTCAG CCGGAAAGGC AAGAACTCCG TGAAGTCAGT GCCAGTGAGC
    GCTGGTGGTG AGGGGGAAAC CTCCCCATAC AGCCTTGAGG CCTCTCCACT GGGGCAGCTC
    ATGAACATGT TATCACACCC AGTCATCCGC CGGAGCTCTC TCTTAACTGA GAAACTCCTC
    AGACTCCTTT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTGTCGGA AGCACAGGCT
    AATTCTGGCA GCAGTGCTTC CTCCACCACT GTTGCCACCT CAACCACATC TACCACCACT
    ACCACTGCTG CCTCCAGCAC GCCCACTCCC CCTGCTGCAA CCACCCCTGT CACTTCTGCT
    CCAGCTCTGG TTGCTGCCAC GGCTATATCC ACCATTGCCG TAGCTGCTTC GACCACGGTG
    ACTACCCCCA CGACTGCTAC CACTACTGTT TCAACTTCTA CAACTACTAA GGCCAGCAAA
    TCTCCAGCAA AGGTGGGTGA TGGGGGCAGC AGCAGTGCTG ACTTTAAGAT GGTATCCTCT
    GGCCTCACTG AAAACCAGCT GCAGCTCTCT GTGGAGGTGT TGACATCCCA CTCTTGTTCC
    GAAGAAGGTC TAGAGGATGC AGCCAATGTG TTACTGCAGC TCTCTCGGGG GGACCCTGCA
    ACCCGGGACA CTGTTCTCAA GCTGCTACTG AATGGAGCGC GCCATCTGGG TTATACTCTT
    TGTAAACAGA TAGGTACCCT TCTGGCTGAG TTACGGGAAT ACAACCTAGA ACAGCAGCGA
    CGAGCCCAAT GTGAGACCCT CTCTCCTGAT GGCCTGCCTG AGGAGCAGCC ACAGACCACC
    AAGCTAAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AGAATGTGGT AATTGTGGCA
    TCTCAGAAGC GACCTCTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA
    TCCAAGACGT CTACCCAGAA GTTCTTCTTG AGGGTACTGC AGGTCATCAT CCAGCTTCGG
    GACGACACAC GCCGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCTTCCGGT
    TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC
    ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG
    GAGTCAAGCC AGTCAGAGGC CTCTGTCCGG AGAGAGGAGT CACCCATGGA TGTGGACCAG
    CCATCTCCCA GTGCTCAGGA TACTCAGTCC ATTGGCTCAG ATGGAACCCC ACAAGGGGAA
    AAGGAGAAGG AGGAGAGGCC ACCTGAGTTA CCCCTGCTCA GTGAGCAGCT GAGCTTGGAC
    GAGCTGTGGG ACATGCTGGG AGAGTGTCTA AAGGAACTGG AGGAATCCCA TGACCAGCAT
    GCAGTGCTAG TGCTACAGCC TGCCGTCGAG GCCTTCTTTT TGGTCCATGC CACAGAGCGG
    GAGAGCAAAC CTCCTGTCAG AGACACCCGT GAGAGCCAGC TGGCACACAT CAAGGACGAG
    CCTCCTCCAC TCTCCCCTGC CCCCTTAACC CCTGCTACTC CTTCCTCCCT TGACCCATTC
    TTCTCCAGGG AGCCCTCCTC TATGCACATC TCCTCAAGCC TGCCCCCTGA TACACAGAAG
    TTCCTTCGCT TTGCAGAGAC TCACCGCACT GTGTTAAATC AGATCCTACG GCAGTCCACC
    ACCCACCTCG CTGATGGGCC TTTTGCTGTC CTGGTAGACT ACATTCGTGT CCTCGACTTT
    GATGTCAAGC GCAAATATTT CCGCCAAGAG CTGGAACGTC TGGATGAGGG GCTCCGGAAA
    GAAGACATGG CTGTGCACGT CCGCCGTGAT CATGTGTTTG AAGACTCCTA TCGTGAACTG
    CACCGGAAAT CCCCTGAAGA AATGAAGAAT CGATTGTATA TAGTGTTTGA AGGAGAAGAA
    GGGCAGGATG CTGGAGGGCT CCTGCGGGAG TGGTATATGA TCATCTCTCG GGAGATGTTT
    AACCCTATGT ATGCCTTGTT CCGTACCTCA CCCGGTGATC GGGTCACCTA CACCATCAAC
    CCATCTTCCC ACTGCAACCC CAATCACCTC AGCTACTTCA AGTTTGTTGG ACGCATTGTG
    GCCAAAGCTG TATATGACAA CCGTCTCCTG GAGTGCTATT TTACTCGGTC CTTCTACAAA
    CACATCTTGG GCAAGTCTGT CAGATATACA GATATGGAGA GTGAAGATTA CCACTTCTAC
    CAGGGCCTGG TTTATCTGCT GGAAAATGAT GTCTCCACAC TAGGTTATGA CCTCACCTTC
    AGCACTGAGG TCCAGGAGTT TGGAGTCTGT GAAGTTCGTG ACCTCAAACC CAATGGGGCT
    AACATCTTGG TAACTGAAGA GAATAAGAAG GAATATGTAC ACCTGGTGTG CCAGATGAGG
    ATGACAGGAG CCATCCGTAA ACAGCTGGCA GCTTTCTTAG AAGGCTTCTA TGAGATCATT
    CCAAAGCGCC TCATTTCTAT CTTCACTGAG CAGGAGTTAG AGCTGCTCAT ATCAGGACTG
    CCCACAATCG ACATTGATGA TTTGAAATCC AACACTGAAT ACCACAAGTA CCAGTCCAAC
    TCTATTCAGA TCCAGTGGTT CTGGAGAGCA TTGCGTTCTT TCGACCAAGC TGACCGTGCC
    AAGTTCCTCC AATTTGTCAC GGGTACTTCA AAGGTGCCCT TACAAGGCTT TGCTGCCCTT
    GAAGGCATGA ATGGCATTCA GAAGTTTCAG ATCCATCGGG ATGACAGGTC CACAGATCGC
    CTGCCTTCAG CTCACACATG TTTTAATCAG CTGGACCTAC CTGCCTATGA GAGCTTTGAA
    AAGCTCCGCC ACATGTTACT ATTGGCCATC CAGGAGTGCT CTGAAGGCTT TGGGCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641438.1
    CDS450..13712
    Translation

    Target ORF information:

    RefSeq Version XM_005641381.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641381.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAC CTCTCTTTCC
    CCAGAGAGGG CTGAAGATTC TGATGCGCTG ACAGCTGTCA GCAGTCAACT GGAAGGCTCT
    CCCATGGACA CCAGTAGCCT GGCTTCCTGT ACCCTAGAGG AGGCTGTGGG TGACACCTCA
    GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGCAGCTCCA CTCCTGGGGA TGCCCCAACT
    GTTGTGACAG AAGTGCAAAG CAGGGGTGAT GGGTCAGGTG AACCTACCCA GCCCCCTGAA
    GACAGCTCTC CCCCTGCATC CTCTGAGAGT TCTTCCACCA GAGATTCTGC TGTGGCCATT
    TCTGGAGCAG ATTCCCGAGG AATCCTAGAA GAGCCACTGC CTTCAACAAG CAGTGAGGAA
    GAAGATCCCC TTGCAGGTAT CAGTCTACCA GAAGGTGTGG ACCCCTCTTT CCTGGCTGCC
    CTGCCTGATG ACATCCGCCG GGAAGTCCTA CAGAACCAAC TGGGCATCCG TCCACCAACT
    CGGACTGCCC CTTCGGCAAA TAGCTCAGCT CCTGCAGTGG TGGGGAATCC TGGTGTGACC
    GAAGTGAGCC CTGAGTTCCT GGCGGCCCTG CCTCCAGCCA TTCAGGAGGA AGTGCTGGCA
    CAGCAGAGAG CTGAGCAACA ACGACGGGAA CTGGCACAGA ATGCGAGCTC AGACACTCCC
    ATGGACCCTG TGACCTTCAT CCAGACCCTG CCATCAGACT TGCGCCGTAG TGTCCTGGAG
    GACATGGAGG ACAGTGTTTT GGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCC
    CTGAGGCGAG AGCAGGAAGC CCGGCAGCGA CAGCTCATGC ATGAGCGGCT CTTTGGCCAC
    AGCAGCACCT CTGCGCTCTC TGCCATCCTC CGAAGCCCGG CTTTCACCAG TCGCCTCAGT
    GGCAACCGAG GAGTCCAATA CACTCGTCTT GCTGTGCAAA GAGGTGGCAC CTTCCAGATG
    GGGGGTAGCA GCAGCCATAA CAGGCCTTCT GGCAGTAATG TGGATACTCT TCTCCGCCTC
    CGAGGACGGC TCCTCCTGGA CCATGAAGCC CTATCCTGTC TCCTGGTCCT ACTTTTCGTG
    GATGAGCCAA AGCTTAATAC TAGTCGTCTA CACCGAGTAC TGAGAAATCT CTGCTACCAT
    GCCCAGACCC GCCACTGGGT CATCCGCAGT CTGCTCTCCA TTTTGCAGCG CAGCAGTGAA
    AGTGAGCTAT GCATTGAAAC ACCCAAGCTC TCCTCAAGTG AGGACAAGGG CAAAAAGTCA
    AGCAAAAGCT GTGGGTCAAG CAGCCATGAG AACCGGCCCC TGGACCTCCT ACACAAGATG
    GAGTCTAAGA GCTCCAACCA GCTTTCCTGG CTCTCAGTAT CCATGGATGC AGCCCTCGGC
    TGCAGGACTA ATATATTCCA GATTCAACGT TCAGGAGGTC GCAAACATAC CGAGAAGCAT
    GCAAGCAGTG GCTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT CTGCAGACAT
    GTTCTAGACA CACTCATTCA ATTGGCCAAG GTATTTCCCA GCCATTTTAC ACAGCAGCGG
    ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAGAGGGGCA GTAAGCAGGC CTGCAGCCCA
    TGCTCTTCAC AGTCCACCAG CAGTGGGATT TGCACAGACT TCTGGGACTT ACTGGTTAAA
    CTGGACAACA TGAATGTCAG CCGGAAAGGC AAGAACTCCG TGAAGTCAGT GCCAGTGAGC
    GCTGGTGGTG AGGGGGAAAC CTCCCCATAC AGCCTTGAGG CCTCTCCACT GGGGCAGCTC
    ATGAACATGT TATCACACCC AGTCATCCGC CGGAGCTCTC TCTTAACTGA GAAACTCCTC
    AGACTCCTTT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTGTCGGA AGCACAGGCT
    AATTCTGGCA GCAGTGCTTC CTCCACCACT GTTGCCACCT CAACCACATC TACCACCACT
    ACCACTGCTG CCTCCAGCAC GCCCACTCCC CCTGCTGCAA CCACCCCTGT CACTTCTGCT
    CCAGCTCTGG TTGCTGCCAC GGCTATATCC ACCATTGCCG TAGCTGCTTC GACCACGGTG
    ACTACCCCCA CGACTGCTAC CACTACTGTT TCAACTTCTA CAACTACTAA GGCCAGCAAA
    TCTCCAGCAA AGGTGGGTGA TGGGGGCAGC AGCAGTGCTG ACTTTAAGAT GGTATCCTCT
    GGCCTCACTG AAAACCAGCT GCAGCTCTCT GTGGAGGTGT TGACATCCCA CTCTTGTTCC
    GAAGAAGGTC TAGAGGATGC AGCCAATGTG TTACTGCAGC TCTCTCGGGG GGACCCTGCA
    ACCCGGGACA CTGTTCTCAA GCTGCTACTG AATGGAGCGC GCCATCTGGG TTATACTCTT
    TGTAAACAGA TAGGTACCCT TCTGGCTGAG TTACGGGAAT ACAACCTAGA ACAGCAGCGA
    CGAGCCCAAT GTGAGACCCT CTCTCCTGAT GGCCTGCCTG AGGAGCAGCC ACAGACCACC
    AAGCTAAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AGAATGTGGT AATTGTGGCA
    TCTCAGAAGC GACCTCTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA
    TCCAAGACGT CTACCCAGAA GTTCTTCTTG AGGGTACTGC AGGTCATCAT CCAGCTTCGG
    GACGACACAC GCCGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCTTCCGGT
    TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC
    ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG
    GAGTCAAGCC AGTCAGAGGC CTCTGTCCGG AGAGAGGAGT CACCCATGGA TGTGGACCAG
    CCATCTCCCA GTGCTCAGGA TACTCAGTCC ATTGGCTCAG ATGGAACCCC ACAAGGGGAA
    AAGGAGAAGG AGGAGAGGCC ACCTGAGTTA CCCCTGCTCA GTGAGCAGCT GAGCTTGGAC
    GAGCTGTGGG ACATGCTGGG AGAGTGTCTA AAGGAACTGG AGGAATCCCA TGACCAGCAT
    GCAGTGCTAG TGCTACAGCC TGCCGTCGAG GCCTTCTTTT TGGTCCATGC CACAGAGCGG
    GAGAGCAAAC CTCCTGTCAG AGACACCCGT GAGAGCCAGC TGGCACACAT CAAGGACGAG
    CCTCCTCCAC TCTCCCCTGC CCCCTTAACC CCTGCTACTC CTTCCTCCCT TGACCCATTC
    TTCTCCAGGG AGCCCTCCTC TATGCACATC TCCTCAAGCC TGCCCCCTGA TACACAGAAG
    TTCCTTCGCT TTGCAGAGAC TCACCGCACT GTGTTAAATC AGATCCTACG GCAGTCCACC
    ACCCACCTCG CTGATGGGCC TTTTGCTGTC CTGGTAGACT ACATTCGTGT CCTCGACTTT
    GATGTCAAGC GCAAATATTT CCGCCAAGAG CTGGAACGTC TGGATGAGGG GCTCCGGAAA
    GAAGACATGG CTGTGCACGT CCGCCGTGAT CATGTGTTTG AAGACTCCTA TCGTGAACTG
    CACCGGAAAT CCCCTGAAGA AATGAAGAAT CGATTGTATA TAGTGTTTGA AGGAGAAGAA
    GGGCAGGATG CTGGAGGGCT CCTGCGGGAG TGGTATATGA TCATCTCTCG GGAGATGTTT
    AACCCTATGT ATGCCTTGTT CCGTACCTCA CCCGGTGATC GGGTCACCTA CACCATCAAC
    CCATCTTCCC ACTGCAACCC CAATCACCTC AGCTACTTCA AGTTTGTTGG ACGCATTGTG
    GCCAAAGCTG TATATGACAA CCGTCTCCTG GAGTGCTATT TTACTCGGTC CTTCTACAAA
    CACATCTTGG GCAAGTCTGT CAGATATACA GATATGGAGA GTGAAGATTA CCACTTCTAC
    CAGGGCCTGG TTTATCTGCT GGAAAATGAT GTCTCCACAC TAGGTTATGA CCTCACCTTC
    AGCACTGAGG TCCAGGAGTT TGGAGTCTGT GAAGTTCGTG ACCTCAAACC CAATGGGGCT
    AACATCTTGG TAACTGAAGA GAATAAGAAG GAATATGTAC ACCTGGTGTG CCAGATGAGG
    ATGACAGGAG CCATCCGTAA ACAGCTGGCA GCTTTCTTAG AAGGCTTCTA TGAGATCATT
    CCAAAGCGCC TCATTTCTAT CTTCACTGAG CAGGAGTTAG AGCTGCTCAT ATCAGGACTG
    CCCACAATCG ACATTGATGA TTTGAAATCC AACACTGAAT ACCACAAGTA CCAGTCCAAC
    TCTATTCAGA TCCAGTGGTT CTGGAGAGCA TTGCGTTCTT TCGACCAAGC TGACCGTGCC
    AAGTTCCTCC AATTTGTCAC GGGTACTTCA AAGGTGCCCT TACAAGGCTT TGCTGCCCTT
    GAAGGCATGA ATGGCATTCA GAAGTTTCAG ATCCATCGGG ATGACAGGTC CACAGATCGC
    CTGCCTTCAG CTCACACATG TTTTAATCAG CTGGACCTAC CTGCCTATGA GAGCTTTGAA
    AAGCTCCGCC ACATGTTACT ATTGGCCATC CAGGAGTGCT CTGAAGGCTT TGGGCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe32412
    Clone ID Related Accession (Same CDS sequence) XM_005641381.2 , XM_005641381.3
    Accession Version XM_005641381.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13263bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005641381.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAC CTCTCTTTCC
    CCAGAGAGGG CTGAAGATTC TGATGCGCTG ACAGCTGTCA GCAGTCAACT GGAAGGCTCT
    CCCATGGACA CCAGTAGCCT GGCTTCCTGT ACCCTAGAGG AGGCTGTGGG TGACACCTCA
    GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGCAGCTCCA CTCCTGGGGA TGCCCCAACT
    GTTGTGACAG AAGTGCAAAG CAGGGGTGAT GGGTCAGGTG AACCTACCCA GCCCCCTGAA
    GACAGCTCTC CCCCTGCATC CTCTGAGAGT TCTTCCACCA GAGATTCTGC TGTGGCCATT
    TCTGGAGCAG ATTCCCGAGG AATCCTAGAA GAGCCACTGC CTTCAACAAG CAGTGAGGAA
    GAAGATCCCC TTGCAGGTAT CAGTCTACCA GAAGGTGTGG ACCCCTCTTT CCTGGCTGCC
    CTGCCTGATG ACATCCGCCG GGAAGTCCTA CAGAACCAAC TGGGCATCCG TCCACCAACT
    CGGACTGCCC CTTCGGCAAA TAGCTCAGCT CCTGCAGTGG TGGGGAATCC TGGTGTGACC
    GAAGTGAGCC CTGAGTTCCT GGCGGCCCTG CCTCCAGCCA TTCAGGAGGA AGTGCTGGCA
    CAGCAGAGAG CTGAGCAACA ACGACGGGAA CTGGCACAGA ATGCGAGCTC AGACACTCCC
    ATGGACCCTG TGACCTTCAT CCAGACCCTG CCATCAGACT TGCGCCGTAG TGTCCTGGAG
    GACATGGAGG ACAGTGTTTT GGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCC
    CTGAGGCGAG AGCAGGAAGC CCGGCAGCGA CAGCTCATGC ATGAGCGGCT CTTTGGCCAC
    AGCAGCACCT CTGCGCTCTC TGCCATCCTC CGAAGCCCGG CTTTCACCAG TCGCCTCAGT
    GGCAACCGAG GAGTCCAATA CACTCGTCTT GCTGTGCAAA GAGGTGGCAC CTTCCAGATG
    GGGGGTAGCA GCAGCCATAA CAGGCCTTCT GGCAGTAATG TGGATACTCT TCTCCGCCTC
    CGAGGACGGC TCCTCCTGGA CCATGAAGCC CTATCCTGTC TCCTGGTCCT ACTTTTCGTG
    GATGAGCCAA AGCTTAATAC TAGTCGTCTA CACCGAGTAC TGAGAAATCT CTGCTACCAT
    GCCCAGACCC GCCACTGGGT CATCCGCAGT CTGCTCTCCA TTTTGCAGCG CAGCAGTGAA
    AGTGAGCTAT GCATTGAAAC ACCCAAGCTC TCCTCAAGTG AGGACAAGGG CAAAAAGTCA
    AGCAAAAGCT GTGGGTCAAG CAGCCATGAG AACCGGCCCC TGGACCTCCT ACACAAGATG
    GAGTCTAAGA GCTCCAACCA GCTTTCCTGG CTCTCAGTAT CCATGGATGC AGCCCTCGGC
    TGCAGGACTA ATATATTCCA GATTCAACGT TCAGGAGGTC GCAAACATAC CGAGAAGCAT
    GCAAGCAGTG GCTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT CTGCAGACAT
    GTTCTAGACA CACTCATTCA ATTGGCCAAG GTATTTCCCA GCCATTTTAC ACAGCAGCGG
    ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAGAGGGGCA GTAAGCAGGC CTGCAGCCCA
    TGCTCTTCAC AGTCCACCAG CAGTGGGATT TGCACAGACT TCTGGGACTT ACTGGTTAAA
    CTGGACAACA TGAATGTCAG CCGGAAAGGC AAGAACTCCG TGAAGTCAGT GCCAGTGAGC
    GCTGGTGGTG AGGGGGAAAC CTCCCCATAC AGCCTTGAGG CCTCTCCACT GGGGCAGCTC
    ATGAACATGT TATCACACCC AGTCATCCGC CGGAGCTCTC TCTTAACTGA GAAACTCCTC
    AGACTCCTTT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTGTCGGA AGCACAGGCT
    AATTCTGGCA GCAGTGCTTC CTCCACCACT GTTGCCACCT CAACCACATC TACCACCACT
    ACCACTGCTG CCTCCAGCAC GCCCACTCCC CCTGCTGCAA CCACCCCTGT CACTTCTGCT
    CCAGCTCTGG TTGCTGCCAC GGCTATATCC ACCATTGCCG TAGCTGCTTC GACCACGGTG
    ACTACCCCCA CGACTGCTAC CACTACTGTT TCAACTTCTA CAACTACTAA GGCCAGCAAA
    TCTCCAGCAA AGGTGGGTGA TGGGGGCAGC AGCAGTGCTG ACTTTAAGAT GGTATCCTCT
    GGCCTCACTG AAAACCAGCT GCAGCTCTCT GTGGAGGTGT TGACATCCCA CTCTTGTTCC
    GAAGAAGGTC TAGAGGATGC AGCCAATGTG TTACTGCAGC TCTCTCGGGG GGACCCTGCA
    ACCCGGGACA CTGTTCTCAA GCTGCTACTG AATGGAGCGC GCCATCTGGG TTATACTCTT
    TGTAAACAGA TAGGTACCCT TCTGGCTGAG TTACGGGAAT ACAACCTAGA ACAGCAGCGA
    CGAGCCCAAT GTGAGACCCT CTCTCCTGAT GGCCTGCCTG AGGAGCAGCC ACAGACCACC
    AAGCTAAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AGAATGTGGT AATTGTGGCA
    TCTCAGAAGC GACCTCTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA
    TCCAAGACGT CTACCCAGAA GTTCTTCTTG AGGGTACTGC AGGTCATCAT CCAGCTTCGG
    GACGACACAC GCCGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCTTCCGGT
    TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC
    ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG
    GAGTCAAGCC AGTCAGAGGC CTCTGTCCGG AGAGAGGAGT CACCCATGGA TGTGGACCAG
    CCATCTCCCA GTGCTCAGGA TACTCAGTCC ATTGGCTCAG ATGGAACCCC ACAAGGGGAA
    AAGGAGAAGG AGGAGAGGCC ACCTGAGTTA CCCCTGCTCA GTGAGCAGCT GAGCTTGGAC
    GAGCTGTGGG ACATGCTGGG AGAGTGTCTA AAGGAACTGG AGGAATCCCA TGACCAGCAT
    GCAGTGCTAG TGCTACAGCC TGCCGTCGAG GCCTTCTTTT TGGTCCATGC CACAGAGCGG
    GAGAGCAAAC CTCCTGTCAG AGACACCCGT GAGAGCCAGC TGGCACACAT CAAGGACGAG
    CCTCCTCCAC TCTCCCCTGC CCCCTTAACC CCTGCTACTC CTTCCTCCCT TGACCCATTC
    TTCTCCAGGG AGCCCTCCTC TATGCACATC TCCTCAAGCC TGCCCCCTGA TACACAGAAG
    TTCCTTCGCT TTGCAGAGAC TCACCGCACT GTGTTAAATC AGATCCTACG GCAGTCCACC
    ACCCACCTCG CTGATGGGCC TTTTGCTGTC CTGGTAGACT ACATTCGTGT CCTCGACTTT
    GATGTCAAGC GCAAATATTT CCGCCAAGAG CTGGAACGTC TGGATGAGGG GCTCCGGAAA
    GAAGACATGG CTGTGCACGT CCGCCGTGAT CATGTGTTTG AAGACTCCTA TCGTGAACTG
    CACCGGAAAT CCCCTGAAGA AATGAAGAAT CGATTGTATA TAGTGTTTGA AGGAGAAGAA
    GGGCAGGATG CTGGAGGGCT CCTGCGGGAG TGGTATATGA TCATCTCTCG GGAGATGTTT
    AACCCTATGT ATGCCTTGTT CCGTACCTCA CCCGGTGATC GGGTCACCTA CACCATCAAC
    CCATCTTCCC ACTGCAACCC CAATCACCTC AGCTACTTCA AGTTTGTTGG ACGCATTGTG
    GCCAAAGCTG TATATGACAA CCGTCTCCTG GAGTGCTATT TTACTCGGTC CTTCTACAAA
    CACATCTTGG GCAAGTCTGT CAGATATACA GATATGGAGA GTGAAGATTA CCACTTCTAC
    CAGGGCCTGG TTTATCTGCT GGAAAATGAT GTCTCCACAC TAGGTTATGA CCTCACCTTC
    AGCACTGAGG TCCAGGAGTT TGGAGTCTGT GAAGTTCGTG ACCTCAAACC CAATGGGGCT
    AACATCTTGG TAACTGAAGA GAATAAGAAG GAATATGTAC ACCTGGTGTG CCAGATGAGG
    ATGACAGGAG CCATCCGTAA ACAGCTGGCA GCTTTCTTAG AAGGCTTCTA TGAGATCATT
    CCAAAGCGCC TCATTTCTAT CTTCACTGAG CAGGAGTTAG AGCTGCTCAT ATCAGGACTG
    CCCACAATCG ACATTGATGA TTTGAAATCC AACACTGAAT ACCACAAGTA CCAGTCCAAC
    TCTATTCAGA TCCAGTGGTT CTGGAGAGCA TTGCGTTCTT TCGACCAAGC TGACCGTGCC
    AAGTTCCTCC AATTTGTCAC GGGTACTTCA AAGGTGCCCT TACAAGGCTT TGCTGCCCTT
    GAAGGCATGA ATGGCATTCA GAAGTTTCAG ATCCATCGGG ATGACAGGTC CACAGATCGC
    CTGCCTTCAG CTCACACATG TTTTAATCAG CTGGACCTAC CTGCCTATGA GAGCTTTGAA
    AAGCTCCGCC ACATGTTACT ATTGGCCATC CAGGAGTGCT CTGAAGGCTT TGGGCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005641438.1
    CDS360..13622
    Translation

    Target ORF information:

    RefSeq Version XM_005641381.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005641381.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAC CTCTCTTTCC
    CCAGAGAGGG CTGAAGATTC TGATGCGCTG ACAGCTGTCA GCAGTCAACT GGAAGGCTCT
    CCCATGGACA CCAGTAGCCT GGCTTCCTGT ACCCTAGAGG AGGCTGTGGG TGACACCTCA
    GCAGCTGGCA GTTCTGAGCA GCCCACAGCA GGCAGCTCCA CTCCTGGGGA TGCCCCAACT
    GTTGTGACAG AAGTGCAAAG CAGGGGTGAT GGGTCAGGTG AACCTACCCA GCCCCCTGAA
    GACAGCTCTC CCCCTGCATC CTCTGAGAGT TCTTCCACCA GAGATTCTGC TGTGGCCATT
    TCTGGAGCAG ATTCCCGAGG AATCCTAGAA GAGCCACTGC CTTCAACAAG CAGTGAGGAA
    GAAGATCCCC TTGCAGGTAT CAGTCTACCA GAAGGTGTGG ACCCCTCTTT CCTGGCTGCC
    CTGCCTGATG ACATCCGCCG GGAAGTCCTA CAGAACCAAC TGGGCATCCG TCCACCAACT
    CGGACTGCCC CTTCGGCAAA TAGCTCAGCT CCTGCAGTGG TGGGGAATCC TGGTGTGACC
    GAAGTGAGCC CTGAGTTCCT GGCGGCCCTG CCTCCAGCCA TTCAGGAGGA AGTGCTGGCA
    CAGCAGAGAG CTGAGCAACA ACGACGGGAA CTGGCACAGA ATGCGAGCTC AGACACTCCC
    ATGGACCCTG TGACCTTCAT CCAGACCCTG CCATCAGACT TGCGCCGTAG TGTCCTGGAG
    GACATGGAGG ACAGTGTTTT GGCTGTGATG CCACCTGACA TTGCAGCTGA GGCTCAAGCC
    CTGAGGCGAG AGCAGGAAGC CCGGCAGCGA CAGCTCATGC ATGAGCGGCT CTTTGGCCAC
    AGCAGCACCT CTGCGCTCTC TGCCATCCTC CGAAGCCCGG CTTTCACCAG TCGCCTCAGT
    GGCAACCGAG GAGTCCAATA CACTCGTCTT GCTGTGCAAA GAGGTGGCAC CTTCCAGATG
    GGGGGTAGCA GCAGCCATAA CAGGCCTTCT GGCAGTAATG TGGATACTCT TCTCCGCCTC
    CGAGGACGGC TCCTCCTGGA CCATGAAGCC CTATCCTGTC TCCTGGTCCT ACTTTTCGTG
    GATGAGCCAA AGCTTAATAC TAGTCGTCTA CACCGAGTAC TGAGAAATCT CTGCTACCAT
    GCCCAGACCC GCCACTGGGT CATCCGCAGT CTGCTCTCCA TTTTGCAGCG CAGCAGTGAA
    AGTGAGCTAT GCATTGAAAC ACCCAAGCTC TCCTCAAGTG AGGACAAGGG CAAAAAGTCA
    AGCAAAAGCT GTGGGTCAAG CAGCCATGAG AACCGGCCCC TGGACCTCCT ACACAAGATG
    GAGTCTAAGA GCTCCAACCA GCTTTCCTGG CTCTCAGTAT CCATGGATGC AGCCCTCGGC
    TGCAGGACTA ATATATTCCA GATTCAACGT TCAGGAGGTC GCAAACATAC CGAGAAGCAT
    GCAAGCAGTG GCTCCACTGT CCACATCCAC CCCCAGGCTG CTCCTGTTGT CTGCAGACAT
    GTTCTAGACA CACTCATTCA ATTGGCCAAG GTATTTCCCA GCCATTTTAC ACAGCAGCGG
    ACCAAAGAAA CAAACTGTGA GAGTGATCGG GAGAGGGGCA GTAAGCAGGC CTGCAGCCCA
    TGCTCTTCAC AGTCCACCAG CAGTGGGATT TGCACAGACT TCTGGGACTT ACTGGTTAAA
    CTGGACAACA TGAATGTCAG CCGGAAAGGC AAGAACTCCG TGAAGTCAGT GCCAGTGAGC
    GCTGGTGGTG AGGGGGAAAC CTCCCCATAC AGCCTTGAGG CCTCTCCACT GGGGCAGCTC
    ATGAACATGT TATCACACCC AGTCATCCGC CGGAGCTCTC TCTTAACTGA GAAACTCCTC
    AGACTCCTTT CTCTCATCTC AATTGCTCTC CCAGAAAACA AAGTGTCGGA AGCACAGGCT
    AATTCTGGCA GCAGTGCTTC CTCCACCACT GTTGCCACCT CAACCACATC TACCACCACT
    ACCACTGCTG CCTCCAGCAC GCCCACTCCC CCTGCTGCAA CCACCCCTGT CACTTCTGCT
    CCAGCTCTGG TTGCTGCCAC GGCTATATCC ACCATTGCCG TAGCTGCTTC GACCACGGTG
    ACTACCCCCA CGACTGCTAC CACTACTGTT TCAACTTCTA CAACTACTAA GGCCAGCAAA
    TCTCCAGCAA AGGTGGGTGA TGGGGGCAGC AGCAGTGCTG ACTTTAAGAT GGTATCCTCT
    GGCCTCACTG AAAACCAGCT GCAGCTCTCT GTGGAGGTGT TGACATCCCA CTCTTGTTCC
    GAAGAAGGTC TAGAGGATGC AGCCAATGTG TTACTGCAGC TCTCTCGGGG GGACCCTGCA
    ACCCGGGACA CTGTTCTCAA GCTGCTACTG AATGGAGCGC GCCATCTGGG TTATACTCTT
    TGTAAACAGA TAGGTACCCT TCTGGCTGAG TTACGGGAAT ACAACCTAGA ACAGCAGCGA
    CGAGCCCAAT GTGAGACCCT CTCTCCTGAT GGCCTGCCTG AGGAGCAGCC ACAGACCACC
    AAGCTAAAAG GCAAAATGCA GAGCAGGTTT GACATGGCTG AGAATGTGGT AATTGTGGCA
    TCTCAGAAGC GACCTCTGGG TGGCCGGGAG CTCCAGCTGC CTTCTATGTC CATGTTGACA
    TCCAAGACGT CTACCCAGAA GTTCTTCTTG AGGGTACTGC AGGTCATCAT CCAGCTTCGG
    GACGACACAC GCCGAGCTAA CAAGAAAGCC AAGCAGACAG GCAGGCTAGG TTCTTCCGGT
    TTAGGCTCAG CTAGCAGCAT CCAGGCAGCT GTTCGGCAGC TGGAGGCTGA GGCTGATGCC
    ATTATACAAA TGGTACGTGA GGGTCAAAGG GCGCGGAGAC AGCAACAAGC AGCAACGTCG
    GAGTCAAGCC AGTCAGAGGC CTCTGTCCGG AGAGAGGAGT CACCCATGGA TGTGGACCAG
    CCATCTCCCA GTGCTCAGGA TACTCAGTCC ATTGGCTCAG ATGGAACCCC ACAAGGGGAA
    AAGGAGAAGG AGGAGAGGCC ACCTGAGTTA CCCCTGCTCA GTGAGCAGCT GAGCTTGGAC
    GAGCTGTGGG ACATGCTGGG AGAGTGTCTA AAGGAACTGG AGGAATCCCA TGACCAGCAT
    GCAGTGCTAG TGCTACAGCC TGCCGTCGAG GCCTTCTTTT TGGTCCATGC CACAGAGCGG
    GAGAGCAAAC CTCCTGTCAG AGACACCCGT GAGAGCCAGC TGGCACACAT CAAGGACGAG
    CCTCCTCCAC TCTCCCCTGC CCCCTTAACC CCTGCTACTC CTTCCTCCCT TGACCCATTC
    TTCTCCAGGG AGCCCTCCTC TATGCACATC TCCTCAAGCC TGCCCCCTGA TACACAGAAG
    TTCCTTCGCT TTGCAGAGAC TCACCGCACT GTGTTAAATC AGATCCTACG GCAGTCCACC
    ACCCACCTCG CTGATGGGCC TTTTGCTGTC CTGGTAGACT ACATTCGTGT CCTCGACTTT
    GATGTCAAGC GCAAATATTT CCGCCAAGAG CTGGAACGTC TGGATGAGGG GCTCCGGAAA
    GAAGACATGG CTGTGCACGT CCGCCGTGAT CATGTGTTTG AAGACTCCTA TCGTGAACTG
    CACCGGAAAT CCCCTGAAGA AATGAAGAAT CGATTGTATA TAGTGTTTGA AGGAGAAGAA
    GGGCAGGATG CTGGAGGGCT CCTGCGGGAG TGGTATATGA TCATCTCTCG GGAGATGTTT
    AACCCTATGT ATGCCTTGTT CCGTACCTCA CCCGGTGATC GGGTCACCTA CACCATCAAC
    CCATCTTCCC ACTGCAACCC CAATCACCTC AGCTACTTCA AGTTTGTTGG ACGCATTGTG
    GCCAAAGCTG TATATGACAA CCGTCTCCTG GAGTGCTATT TTACTCGGTC CTTCTACAAA
    CACATCTTGG GCAAGTCTGT CAGATATACA GATATGGAGA GTGAAGATTA CCACTTCTAC
    CAGGGCCTGG TTTATCTGCT GGAAAATGAT GTCTCCACAC TAGGTTATGA CCTCACCTTC
    AGCACTGAGG TCCAGGAGTT TGGAGTCTGT GAAGTTCGTG ACCTCAAACC CAATGGGGCT
    AACATCTTGG TAACTGAAGA GAATAAGAAG GAATATGTAC ACCTGGTGTG CCAGATGAGG
    ATGACAGGAG CCATCCGTAA ACAGCTGGCA GCTTTCTTAG AAGGCTTCTA TGAGATCATT
    CCAAAGCGCC TCATTTCTAT CTTCACTGAG CAGGAGTTAG AGCTGCTCAT ATCAGGACTG
    CCCACAATCG ACATTGATGA TTTGAAATCC AACACTGAAT ACCACAAGTA CCAGTCCAAC
    TCTATTCAGA TCCAGTGGTT CTGGAGAGCA TTGCGTTCTT TCGACCAAGC TGACCGTGCC
    AAGTTCCTCC AATTTGTCAC GGGTACTTCA AAGGTGCCCT TACAAGGCTT TGCTGCCCTT
    GAAGGCATGA ATGGCATTCA GAAGTTTCAG ATCCATCGGG ATGACAGGTC CACAGATCGC
    CTGCCTTCAG CTCACACATG TTTTAATCAG CTGGACCTAC CTGCCTATGA GAGCTTTGAA
    AAGCTCCGCC ACATGTTACT ATTGGCCATC CAGGAGTGCT CTGAAGGCTT TGGGCTGGCC
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45532
    Clone ID Related Accession (Same CDS sequence) XM_014111525.1 , XM_014111524.1 , XM_014111521.2 , XM_014111523.1 , XM_014111522.2 , XM_014111522.1 , XM_014111521.1 , XM_014111523.2 , XM_014111524.2
    Accession Version XM_014111525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
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    2281
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    2581
    2641
    2701
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    12001
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    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967000.1
    CDS50..13348
    Translation

    Target ORF information:

    RefSeq Version XM_014111525.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45532
    Clone ID Related Accession (Same CDS sequence) XM_014111525.1 , XM_014111524.1 , XM_014111521.2 , XM_014111523.1 , XM_014111522.2 , XM_014111522.1 , XM_014111521.1 , XM_014111523.2 , XM_014111524.2
    Accession Version XM_014111524.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
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    2641
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    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966999.1
    CDS258..13556
    Translation

    Target ORF information:

    RefSeq Version XM_014111524.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45532
    Clone ID Related Accession (Same CDS sequence) XM_014111525.1 , XM_014111524.1 , XM_014111521.2 , XM_014111523.1 , XM_014111522.2 , XM_014111522.1 , XM_014111521.1 , XM_014111523.2 , XM_014111524.2
    Accession Version XM_014111521.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111521.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
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    3721
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4621
    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
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    6901
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    7021
    7081
    7141
    7201
    7261
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    7381
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    7501
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    7741
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    9061
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    9181
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    9301
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    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966996.1
    CDS361..13659
    Translation

    Target ORF information:

    RefSeq Version XM_014111521.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111521.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45532
    Clone ID Related Accession (Same CDS sequence) XM_014111525.1 , XM_014111524.1 , XM_014111521.2 , XM_014111523.1 , XM_014111522.2 , XM_014111522.1 , XM_014111521.1 , XM_014111523.2 , XM_014111524.2
    Accession Version XM_014111523.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
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    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966998.1
    CDS287..13585
    Translation

    Target ORF information:

    RefSeq Version XM_014111523.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111523.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45532
    Clone ID Related Accession (Same CDS sequence) XM_014111525.1 , XM_014111524.1 , XM_014111521.2 , XM_014111523.1 , XM_014111522.2 , XM_014111522.1 , XM_014111521.1 , XM_014111523.2 , XM_014111524.2
    Accession Version XM_014111522.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111522.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966997.1
    CDS407..13705
    Translation

    Target ORF information:

    RefSeq Version XM_014111522.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111522.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45532
    Clone ID Related Accession (Same CDS sequence) XM_014111525.1 , XM_014111524.1 , XM_014111521.2 , XM_014111523.1 , XM_014111522.2 , XM_014111522.1 , XM_014111521.1 , XM_014111523.2 , XM_014111524.2
    Accession Version XM_014111522.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966997.1
    CDS395..13693
    Translation

    Target ORF information:

    RefSeq Version XM_014111522.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111522.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45532
    Clone ID Related Accession (Same CDS sequence) XM_014111525.1 , XM_014111524.1 , XM_014111521.2 , XM_014111523.1 , XM_014111522.2 , XM_014111522.1 , XM_014111521.1 , XM_014111523.2 , XM_014111524.2
    Accession Version XM_014111521.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966996.1
    CDS451..13749
    Translation

    Target ORF information:

    RefSeq Version XM_014111521.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111521.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45532
    Clone ID Related Accession (Same CDS sequence) XM_014111525.1 , XM_014111524.1 , XM_014111521.2 , XM_014111523.1 , XM_014111522.2 , XM_014111522.1 , XM_014111521.1 , XM_014111523.2 , XM_014111524.2
    Accession Version XM_014111523.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111523.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1501
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    12661
    12721
    12781
    12841
    12901
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    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966998.1
    CDS303..13601
    Translation

    Target ORF information:

    RefSeq Version XM_014111523.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111523.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45532
    Clone ID Related Accession (Same CDS sequence) XM_014111525.1 , XM_014111524.1 , XM_014111521.2 , XM_014111523.1 , XM_014111522.2 , XM_014111522.1 , XM_014111521.1 , XM_014111523.2 , XM_014111524.2
    Accession Version XM_014111524.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111524.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1261
    1321
    1381
    1441
    1501
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    1981
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    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966999.1
    CDS270..13568
    Translation

    Target ORF information:

    RefSeq Version XM_014111524.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111524.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    13261
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGGT ACGTGAGGGT
    CAAAGGGCGC GGAGACAGCA ACAAGCAGCA ACGTCGGAGT CAAGCCAGTC AGAGGCCTCT
    GTCCGGAGAG AGGAGTCACC CATGGATGTG GACCAGCCAT CTCCCAGTGC TCAGGATACT
    CAGTCCATTG GCTCAGATGG AACCCCACAA GGGGAAAAGG AGAAGGAGGA GAGGCCACCT
    GAGTTACCCC TGCTCAGTGA GCAGCTGAGC TTGGACGAGC TGTGGGACAT GCTGGGAGAG
    TGTCTAAAGG AACTGGAGGA ATCCCATGAC CAGCATGCAG TGCTAGTGCT ACAGCCTGCC
    GTCGAGGCCT TCTTTTTGGT CCATGCCACA GAGCGGGAGA GCAAACCTCC TGTCAGAGAC
    ACCCGTGAGA GCCAGCTGGC ACACATCAAG GACGAGCCTC CTCCACTCTC CCCTGCCCCC
    TTAACCCCTG CTACTCCTTC CTCCCTTGAC CCATTCTTCT CCAGGGAGCC CTCCTCTATG
    CACATCTCCT CAAGCCTGCC CCCTGATACA CAGAAGTTCC TTCGCTTTGC AGAGACTCAC
    CGCACTGTGT TAAATCAGAT CCTACGGCAG TCCACCACCC ACCTCGCTGA TGGGCCTTTT
    GCTGTCCTGG TAGACTACAT TCGTGTCCTC GACTTTGATG TCAAGCGCAA ATATTTCCGC
    CAAGAGCTGG AACGTCTGGA TGAGGGGCTC CGGAAAGAAG ACATGGCTGT GCACGTCCGC
    CGTGATCATG TGTTTGAAGA CTCCTATCGT GAACTGCACC GGAAATCCCC TGAAGAAATG
    AAGAATCGAT TGTATATAGT GTTTGAAGGA GAAGAAGGGC AGGATGCTGG AGGGCTCCTG
    CGGGAGTGGT ATATGATCAT CTCTCGGGAG ATGTTTAACC CTATGTATGC CTTGTTCCGT
    ACCTCACCCG GTGATCGGGT CACCTACACC ATCAACCCAT CTTCCCACTG CAACCCCAAT
    CACCTCAGCT ACTTCAAGTT TGTTGGACGC ATTGTGGCCA AAGCTGTATA TGACAACCGT
    CTCCTGGAGT GCTATTTTAC TCGGTCCTTC TACAAACACA TCTTGGGCAA GTCTGTCAGA
    TATACAGATA TGGAGAGTGA AGATTACCAC TTCTACCAGG GCCTGGTTTA TCTGCTGGAA
    AATGATGTCT CCACACTAGG TTATGACCTC ACCTTCAGCA CTGAGGTCCA GGAGTTTGGA
    GTCTGTGAAG TTCGTGACCT CAAACCCAAT GGGGCTAACA TCTTGGTAAC TGAAGAGAAT
    AAGAAGGAAT ATGTACACCT GGTGTGCCAG ATGAGGATGA CAGGAGCCAT CCGTAAACAG
    CTGGCAGCTT TCTTAGAAGG CTTCTATGAG ATCATTCCAA AGCGCCTCAT TTCTATCTTC
    ACTGAGCAGG AGTTAGAGCT GCTCATATCA GGACTGCCCA CAATCGACAT TGATGATTTG
    AAATCCAACA CTGAATACCA CAAGTACCAG TCCAACTCTA TTCAGATCCA GTGGTTCTGG
    AGAGCATTGC GTTCTTTCGA CCAAGCTGAC CGTGCCAAGT TCCTCCAATT TGTCACGGGT
    ACTTCAAAGG TGCCCTTACA AGGCTTTGCT GCCCTTGAAG GCATGAATGG CATTCAGAAG
    TTTCAGATCC ATCGGGATGA CAGGTCCACA GATCGCCTGC CTTCAGCTCA CACATGTTTT
    AATCAGCTGG ACCTACCTGC CTATGAGAGC TTTGAAAAGC TCCGCCACAT GTTACTATTG
    GCCATCCAGG AGTGCTCTGA AGGCTTTGGG CTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45533
    Clone ID Related Accession (Same CDS sequence) XM_014111526.2 , XM_014111526.1
    Accession Version XM_014111526.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111526.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
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    3001
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    9781
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    10501
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    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGTC GGAGTCAAGC
    CAGTCAGAGG CCTCTGTCCG GAGAGAGGAG TCACCCATGG ATGTGGACCA GCCATCTCCC
    AGTGCTCAGG ATACTCAGTC CATTGGCTCA GATGGAACCC CACAAGGGGA AAAGGAGAAG
    GAGGAGAGGC CACCTGAGTT ACCCCTGCTC AGTGAGCAGC TGAGCTTGGA CGAGCTGTGG
    GACATGCTGG GAGAGTGTCT AAAGGAACTG GAGGAATCCC ATGACCAGCA TGCAGTGCTA
    GTGCTACAGC CTGCCGTCGA GGCCTTCTTT TTGGTCCATG CCACAGAGCG GGAGAGCAAA
    CCTCCTGTCA GAGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCTCCTCCA
    CTCTCCCCTG CCCCCTTAAC CCCTGCTACT CCTTCCTCCC TTGACCCATT CTTCTCCAGG
    GAGCCCTCCT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ATACACAGAA GTTCCTTCGC
    TTTGCAGAGA CTCACCGCAC TGTGTTAAAT CAGATCCTAC GGCAGTCCAC CACCCACCTC
    GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT TGATGTCAAG
    CGCAAATATT TCCGCCAAGA GCTGGAACGT CTGGATGAGG GGCTCCGGAA AGAAGACATG
    GCTGTGCACG TCCGCCGTGA TCATGTGTTT GAAGACTCCT ATCGTGAACT GCACCGGAAA
    TCCCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTGTTTG AAGGAGAAGA AGGGCAGGAT
    GCTGGAGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GGGAGATGTT TAACCCTATG
    TATGCCTTGT TCCGTACCTC ACCCGGTGAT CGGGTCACCT ACACCATCAA CCCATCTTCC
    CACTGCAACC CCAATCACCT CAGCTACTTC AAGTTTGTTG GACGCATTGT GGCCAAAGCT
    GTATATGACA ACCGTCTCCT GGAGTGCTAT TTTACTCGGT CCTTCTACAA ACACATCTTG
    GGCAAGTCTG TCAGATATAC AGATATGGAG AGTGAAGATT ACCACTTCTA CCAGGGCCTG
    GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGTTATG ACCTCACCTT CAGCACTGAG
    GTCCAGGAGT TTGGAGTCTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC TAACATCTTG
    GTAACTGAAG AGAATAAGAA GGAATATGTA CACCTGGTGT GCCAGATGAG GATGACAGGA
    GCCATCCGTA AACAGCTGGC AGCTTTCTTA GAAGGCTTCT ATGAGATCAT TCCAAAGCGC
    CTCATTTCTA TCTTCACTGA GCAGGAGTTA GAGCTGCTCA TATCAGGACT GCCCACAATC
    GACATTGATG ATTTGAAATC CAACACTGAA TACCACAAGT ACCAGTCCAA CTCTATTCAG
    ATCCAGTGGT TCTGGAGAGC ATTGCGTTCT TTCGACCAAG CTGACCGTGC CAAGTTCCTC
    CAATTTGTCA CGGGTACTTC AAAGGTGCCC TTACAAGGCT TTGCTGCCCT TGAAGGCATG
    AATGGCATTC AGAAGTTTCA GATCCATCGG GATGACAGGT CCACAGATCG CCTGCCTTCA
    GCTCACACAT GTTTTAATCA GCTGGACCTA CCTGCCTATG AGAGCTTTGA AAAGCTCCGC
    CACATGTTAC TATTGGCCAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967001.1
    CDS361..13614
    Translation

    Target ORF information:

    RefSeq Version XM_014111526.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111526.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGTC GGAGTCAAGC
    CAGTCAGAGG CCTCTGTCCG GAGAGAGGAG TCACCCATGG ATGTGGACCA GCCATCTCCC
    AGTGCTCAGG ATACTCAGTC CATTGGCTCA GATGGAACCC CACAAGGGGA AAAGGAGAAG
    GAGGAGAGGC CACCTGAGTT ACCCCTGCTC AGTGAGCAGC TGAGCTTGGA CGAGCTGTGG
    GACATGCTGG GAGAGTGTCT AAAGGAACTG GAGGAATCCC ATGACCAGCA TGCAGTGCTA
    GTGCTACAGC CTGCCGTCGA GGCCTTCTTT TTGGTCCATG CCACAGAGCG GGAGAGCAAA
    CCTCCTGTCA GAGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCTCCTCCA
    CTCTCCCCTG CCCCCTTAAC CCCTGCTACT CCTTCCTCCC TTGACCCATT CTTCTCCAGG
    GAGCCCTCCT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ATACACAGAA GTTCCTTCGC
    TTTGCAGAGA CTCACCGCAC TGTGTTAAAT CAGATCCTAC GGCAGTCCAC CACCCACCTC
    GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT TGATGTCAAG
    CGCAAATATT TCCGCCAAGA GCTGGAACGT CTGGATGAGG GGCTCCGGAA AGAAGACATG
    GCTGTGCACG TCCGCCGTGA TCATGTGTTT GAAGACTCCT ATCGTGAACT GCACCGGAAA
    TCCCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTGTTTG AAGGAGAAGA AGGGCAGGAT
    GCTGGAGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GGGAGATGTT TAACCCTATG
    TATGCCTTGT TCCGTACCTC ACCCGGTGAT CGGGTCACCT ACACCATCAA CCCATCTTCC
    CACTGCAACC CCAATCACCT CAGCTACTTC AAGTTTGTTG GACGCATTGT GGCCAAAGCT
    GTATATGACA ACCGTCTCCT GGAGTGCTAT TTTACTCGGT CCTTCTACAA ACACATCTTG
    GGCAAGTCTG TCAGATATAC AGATATGGAG AGTGAAGATT ACCACTTCTA CCAGGGCCTG
    GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGTTATG ACCTCACCTT CAGCACTGAG
    GTCCAGGAGT TTGGAGTCTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC TAACATCTTG
    GTAACTGAAG AGAATAAGAA GGAATATGTA CACCTGGTGT GCCAGATGAG GATGACAGGA
    GCCATCCGTA AACAGCTGGC AGCTTTCTTA GAAGGCTTCT ATGAGATCAT TCCAAAGCGC
    CTCATTTCTA TCTTCACTGA GCAGGAGTTA GAGCTGCTCA TATCAGGACT GCCCACAATC
    GACATTGATG ATTTGAAATC CAACACTGAA TACCACAAGT ACCAGTCCAA CTCTATTCAG
    ATCCAGTGGT TCTGGAGAGC ATTGCGTTCT TTCGACCAAG CTGACCGTGC CAAGTTCCTC
    CAATTTGTCA CGGGTACTTC AAAGGTGCCC TTACAAGGCT TTGCTGCCCT TGAAGGCATG
    AATGGCATTC AGAAGTTTCA GATCCATCGG GATGACAGGT CCACAGATCG CCTGCCTTCA
    GCTCACACAT GTTTTAATCA GCTGGACCTA CCTGCCTATG AGAGCTTTGA AAAGCTCCGC
    CACATGTTAC TATTGGCCAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45533
    Clone ID Related Accession (Same CDS sequence) XM_014111526.2 , XM_014111526.1
    Accession Version XM_014111526.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGTC GGAGTCAAGC
    CAGTCAGAGG CCTCTGTCCG GAGAGAGGAG TCACCCATGG ATGTGGACCA GCCATCTCCC
    AGTGCTCAGG ATACTCAGTC CATTGGCTCA GATGGAACCC CACAAGGGGA AAAGGAGAAG
    GAGGAGAGGC CACCTGAGTT ACCCCTGCTC AGTGAGCAGC TGAGCTTGGA CGAGCTGTGG
    GACATGCTGG GAGAGTGTCT AAAGGAACTG GAGGAATCCC ATGACCAGCA TGCAGTGCTA
    GTGCTACAGC CTGCCGTCGA GGCCTTCTTT TTGGTCCATG CCACAGAGCG GGAGAGCAAA
    CCTCCTGTCA GAGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCTCCTCCA
    CTCTCCCCTG CCCCCTTAAC CCCTGCTACT CCTTCCTCCC TTGACCCATT CTTCTCCAGG
    GAGCCCTCCT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ATACACAGAA GTTCCTTCGC
    TTTGCAGAGA CTCACCGCAC TGTGTTAAAT CAGATCCTAC GGCAGTCCAC CACCCACCTC
    GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT TGATGTCAAG
    CGCAAATATT TCCGCCAAGA GCTGGAACGT CTGGATGAGG GGCTCCGGAA AGAAGACATG
    GCTGTGCACG TCCGCCGTGA TCATGTGTTT GAAGACTCCT ATCGTGAACT GCACCGGAAA
    TCCCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTGTTTG AAGGAGAAGA AGGGCAGGAT
    GCTGGAGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GGGAGATGTT TAACCCTATG
    TATGCCTTGT TCCGTACCTC ACCCGGTGAT CGGGTCACCT ACACCATCAA CCCATCTTCC
    CACTGCAACC CCAATCACCT CAGCTACTTC AAGTTTGTTG GACGCATTGT GGCCAAAGCT
    GTATATGACA ACCGTCTCCT GGAGTGCTAT TTTACTCGGT CCTTCTACAA ACACATCTTG
    GGCAAGTCTG TCAGATATAC AGATATGGAG AGTGAAGATT ACCACTTCTA CCAGGGCCTG
    GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGTTATG ACCTCACCTT CAGCACTGAG
    GTCCAGGAGT TTGGAGTCTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC TAACATCTTG
    GTAACTGAAG AGAATAAGAA GGAATATGTA CACCTGGTGT GCCAGATGAG GATGACAGGA
    GCCATCCGTA AACAGCTGGC AGCTTTCTTA GAAGGCTTCT ATGAGATCAT TCCAAAGCGC
    CTCATTTCTA TCTTCACTGA GCAGGAGTTA GAGCTGCTCA TATCAGGACT GCCCACAATC
    GACATTGATG ATTTGAAATC CAACACTGAA TACCACAAGT ACCAGTCCAA CTCTATTCAG
    ATCCAGTGGT TCTGGAGAGC ATTGCGTTCT TTCGACCAAG CTGACCGTGC CAAGTTCCTC
    CAATTTGTCA CGGGTACTTC AAAGGTGCCC TTACAAGGCT TTGCTGCCCT TGAAGGCATG
    AATGGCATTC AGAAGTTTCA GATCCATCGG GATGACAGGT CCACAGATCG CCTGCCTTCA
    GCTCACACAT GTTTTAATCA GCTGGACCTA CCTGCCTATG AGAGCTTTGA AAAGCTCCGC
    CACATGTTAC TATTGGCCAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967001.1
    CDS451..13704
    Translation

    Target ORF information:

    RefSeq Version XM_014111526.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG TGAGCAGAAG TGGAGAGGAT
    GAATCACAAG AGGACGTGCT GATGGATGAA GCTCCTTCCA ACCTCAGCCA AGCTTCCACC
    TTGCAGGCCA ACCGAGAAGA TTCCATGAAT ATCCTGGACC CTGAGGATGA GGAGGAGCAC
    ACTCAGGAAG AGGACAGCAG TGGCAGTAAC GAGGATGAGG ATGATAGTCA GGATGAAGAG
    GAGGAGGAGG AGGAAGATGA GGAAGATGAT CAGGAGGATG ATGAAGGTGA AGAGGGAGAT
    GAAGACGATG ACGACGATGG CTCTGAAATG GAATTGGATG AGGATTACCC TGATATGAAC
    GCTTCTCCCT TGGTCCGATT TGAGCGCTTT GACCGGGAGG ATGATCTCAT CATTGAGTTT
    GACAACATGT TCTCCAGCGC TACAGACATC CCCCCATCCC CAGGAAATAT CCCTACCACT
    CACCCACTGA TGGTACGCCA TGCAGACCAC AGTTCTCTGA CACTGGGCAG TGGCTCCTCC
    ACAACACGTC TCACCCAGGG CATCGGGCGC AGTCAGAGGA CCCTAAGGCA GCTGACAGCC
    AATACAGGCC ACACCATTCA TGTTCATTAC CCTGGGAACC GCCAGCCCAA CCCTCCTCTT
    ATACTCCAGA GGTTGCTTGG TCCTTCTGCT GCTGCTGACA TCCTTCAGCT GAGCAGCAGC
    CTTCCCCTAC AAAGCCGTGG ACGAGCCCGC CTCCTGGTAG GCAATGATGA TGTCCACATC
    ATTGCCAGAT CTGATGATGA ACTGCTGGAT GACTTTTTCC ATGACCAGAG CACAGCTACC
    AGCCAAGCAG GGACCCTATC CAGCATCCCC ACAGCCCTGA CCCGCTGGAC AGAGGAATGC
    AAAGTTCTTG ATGCCGAGAG CATGCATGAC TGTGTTTCAG TTGTTAAAGT CCCCATTGTC
    AATCACTTGG AATTCCTGAG GGATGAGGAG CTGGAAGAAA GGCGGGAGAA ACGCAGGAAA
    CAATTGGCTG AGGAAGAAAC AAAGATAACT GACAAAGGCA AAGAAGATAA GGAGAACAGG
    GATCAGAGTG CACAGTGTAC TGCATCTAAA ACAAATGACT CCGCTGAACA GAACCTCTCA
    GATGGGACTC CTATGCCTGA CAGCTACCCA ACAACCCCAT CTTCAACTGA TGCAGCCACA
    TCTGAGTCCA AGGATACCCT TGTCACTCTG CAGCCCTCAC AACAGCAACA AACACTCCCA
    CCCCCACCAG CCTTGGGAGA GATTCCTCAA GAGCTACAGT CCCCGGCTGG AGAAGGGGGG
    AGCTCTACAC AGCTGTTGAT GCCTGTAGAG CCAGAGGAAT TGGGTCCCAC AAGGCCAAGT
    GGAGAAGCAG AAACAACTCA GATGGAGTTG TCCCCAGCTC CTACTATAAG CAGAGATCTA
    ACCTCTCCAT GTGACTTCCT CCTAGCCTCT CTTTCCCCAG AGAGGGCTGA AGATTCTGAT
    GCGCTGACAG CTGTCAGCAG TCAACTGGAA GGCTCTCCCA TGGACACCAG TAGCCTGGCT
    TCCTGTACCC TAGAGGAGGC TGTGGGTGAC ACCTCAGCAG CTGGCAGTTC TGAGCAGCCC
    ACAGCAGGCA GCTCCACTCC TGGGGATGCC CCAACTGTTG TGACAGAAGT GCAAAGCAGG
    GGTGATGGGT CAGGTGAACC TACCCAGCCC CCTGAAGACA GCTCTCCCCC TGCATCCTCT
    GAGAGTTCTT CCACCAGAGA TTCTGCTGTG GCCATTTCTG GAGCAGATTC CCGAGGAATC
    CTAGAAGAGC CACTGCCTTC AACAAGCAGT GAGGAAGAAG ATCCCCTTGC AGGTATCAGT
    CTACCAGAAG GTGTGGACCC CTCTTTCCTG GCTGCCCTGC CTGATGACAT CCGCCGGGAA
    GTCCTACAGA ACCAACTGGG CATCCGTCCA CCAACTCGGA CTGCCCCTTC GGCAAATAGC
    TCAGCTCCTG CAGTGGTGGG GAATCCTGGT GTGACCGAAG TGAGCCCTGA GTTCCTGGCG
    GCCCTGCCTC CAGCCATTCA GGAGGAAGTG CTGGCACAGC AGAGAGCTGA GCAACAACGA
    CGGGAACTGG CACAGAATGC GAGCTCAGAC ACTCCCATGG ACCCTGTGAC CTTCATCCAG
    ACCCTGCCAT CAGACTTGCG CCGTAGTGTC CTGGAGGACA TGGAGGACAG TGTTTTGGCT
    GTGATGCCAC CTGACATTGC AGCTGAGGCT CAAGCCCTGA GGCGAGAGCA GGAAGCCCGG
    CAGCGACAGC TCATGCATGA GCGGCTCTTT GGCCACAGCA GCACCTCTGC GCTCTCTGCC
    ATCCTCCGAA GCCCGGCTTT CACCAGTCGC CTCAGTGGCA ACCGAGGAGT CCAATACACT
    CGTCTTGCTG TGCAAAGAGG TGGCACCTTC CAGATGGGGG GTAGCAGCAG CCATAACAGG
    CCTTCTGGCA GTAATGTGGA TACTCTTCTC CGCCTCCGAG GACGGCTCCT CCTGGACCAT
    GAAGCCCTAT CCTGTCTCCT GGTCCTACTT TTCGTGGATG AGCCAAAGCT TAATACTAGT
    CGTCTACACC GAGTACTGAG AAATCTCTGC TACCATGCCC AGACCCGCCA CTGGGTCATC
    CGCAGTCTGC TCTCCATTTT GCAGCGCAGC AGTGAAAGTG AGCTATGCAT TGAAACACCC
    AAGCTCTCCT CAAGTGAGGA CAAGGGCAAA AAGTCAAGCA AAAGCTGTGG GTCAAGCAGC
    CATGAGAACC GGCCCCTGGA CCTCCTACAC AAGATGGAGT CTAAGAGCTC CAACCAGCTT
    TCCTGGCTCT CAGTATCCAT GGATGCAGCC CTCGGCTGCA GGACTAATAT ATTCCAGATT
    CAACGTTCAG GAGGTCGCAA ACATACCGAG AAGCATGCAA GCAGTGGCTC CACTGTCCAC
    ATCCACCCCC AGGCTGCTCC TGTTGTCTGC AGACATGTTC TAGACACACT CATTCAATTG
    GCCAAGGTAT TTCCCAGCCA TTTTACACAG CAGCGGACCA AAGAAACAAA CTGTGAGAGT
    GATCGGGAGA GGGGCAGTAA GCAGGCCTGC AGCCCATGCT CTTCACAGTC CACCAGCAGT
    GGGATTTGCA CAGACTTCTG GGACTTACTG GTTAAACTGG ACAACATGAA TGTCAGCCGG
    AAAGGCAAGA ACTCCGTGAA GTCAGTGCCA GTGAGCGCTG GTGGTGAGGG GGAAACCTCC
    CCATACAGCC TTGAGGCCTC TCCACTGGGG CAGCTCATGA ACATGTTATC ACACCCAGTC
    ATCCGCCGGA GCTCTCTCTT AACTGAGAAA CTCCTCAGAC TCCTTTCTCT CATCTCAATT
    GCTCTCCCAG AAAACAAAGT GTCGGAAGCA CAGGCTAATT CTGGCAGCAG TGCTTCCTCC
    ACCACTGTTG CCACCTCAAC CACATCTACC ACCACTACCA CTGCTGCCTC CAGCACGCCC
    ACTCCCCCTG CTGCAACCAC CCCTGTCACT TCTGCTCCAG CTCTGGTTGC TGCCACGGCT
    ATATCCACCA TTGCCGTAGC TGCTTCGACC ACGGTGACTA CCCCCACGAC TGCTACCACT
    ACTGTTTCAA CTTCTACAAC TACTAAGGCC AGCAAATCTC CAGCAAAGGT GGGTGATGGG
    GGCAGCAGCA GTGCTGACTT TAAGATGGTA TCCTCTGGCC TCACTGAAAA CCAGCTGCAG
    CTCTCTGTGG AGGTGTTGAC ATCCCACTCT TGTTCCGAAG AAGGTCTAGA GGATGCAGCC
    AATGTGTTAC TGCAGCTCTC TCGGGGGGAC CCTGCAACCC GGGACACTGT TCTCAAGCTG
    CTACTGAATG GAGCGCGCCA TCTGGGTTAT ACTCTTTGTA AACAGATAGG TACCCTTCTG
    GCTGAGTTAC GGGAATACAA CCTAGAACAG CAGCGACGAG CCCAATGTGA GACCCTCTCT
    CCTGATGGCC TGCCTGAGGA GCAGCCACAG ACCACCAAGC TAAAAGGCAA AATGCAGAGC
    AGGTTTGACA TGGCTGAGAA TGTGGTAATT GTGGCATCTC AGAAGCGACC TCTGGGTGGC
    CGGGAGCTCC AGCTGCCTTC TATGTCCATG TTGACATCCA AGACGTCTAC CCAGAAGTTC
    TTCTTGAGGG TACTGCAGGT CATCATCCAG CTTCGGGACG ACACACGCCG AGCTAACAAG
    AAAGCCAAGC AGACAGGCAG GCTAGGTTCT TCCGGTTTAG GCTCAGCTAG CAGCATCCAG
    GCAGCTGTTC GGCAGCTGGA GGCTGAGGCT GATGCCATTA TACAAATGTC GGAGTCAAGC
    CAGTCAGAGG CCTCTGTCCG GAGAGAGGAG TCACCCATGG ATGTGGACCA GCCATCTCCC
    AGTGCTCAGG ATACTCAGTC CATTGGCTCA GATGGAACCC CACAAGGGGA AAAGGAGAAG
    GAGGAGAGGC CACCTGAGTT ACCCCTGCTC AGTGAGCAGC TGAGCTTGGA CGAGCTGTGG
    GACATGCTGG GAGAGTGTCT AAAGGAACTG GAGGAATCCC ATGACCAGCA TGCAGTGCTA
    GTGCTACAGC CTGCCGTCGA GGCCTTCTTT TTGGTCCATG CCACAGAGCG GGAGAGCAAA
    CCTCCTGTCA GAGACACCCG TGAGAGCCAG CTGGCACACA TCAAGGACGA GCCTCCTCCA
    CTCTCCCCTG CCCCCTTAAC CCCTGCTACT CCTTCCTCCC TTGACCCATT CTTCTCCAGG
    GAGCCCTCCT CTATGCACAT CTCCTCAAGC CTGCCCCCTG ATACACAGAA GTTCCTTCGC
    TTTGCAGAGA CTCACCGCAC TGTGTTAAAT CAGATCCTAC GGCAGTCCAC CACCCACCTC
    GCTGATGGGC CTTTTGCTGT CCTGGTAGAC TACATTCGTG TCCTCGACTT TGATGTCAAG
    CGCAAATATT TCCGCCAAGA GCTGGAACGT CTGGATGAGG GGCTCCGGAA AGAAGACATG
    GCTGTGCACG TCCGCCGTGA TCATGTGTTT GAAGACTCCT ATCGTGAACT GCACCGGAAA
    TCCCCTGAAG AAATGAAGAA TCGATTGTAT ATAGTGTTTG AAGGAGAAGA AGGGCAGGAT
    GCTGGAGGGC TCCTGCGGGA GTGGTATATG ATCATCTCTC GGGAGATGTT TAACCCTATG
    TATGCCTTGT TCCGTACCTC ACCCGGTGAT CGGGTCACCT ACACCATCAA CCCATCTTCC
    CACTGCAACC CCAATCACCT CAGCTACTTC AAGTTTGTTG GACGCATTGT GGCCAAAGCT
    GTATATGACA ACCGTCTCCT GGAGTGCTAT TTTACTCGGT CCTTCTACAA ACACATCTTG
    GGCAAGTCTG TCAGATATAC AGATATGGAG AGTGAAGATT ACCACTTCTA CCAGGGCCTG
    GTTTATCTGC TGGAAAATGA TGTCTCCACA CTAGGTTATG ACCTCACCTT CAGCACTGAG
    GTCCAGGAGT TTGGAGTCTG TGAAGTTCGT GACCTCAAAC CCAATGGGGC TAACATCTTG
    GTAACTGAAG AGAATAAGAA GGAATATGTA CACCTGGTGT GCCAGATGAG GATGACAGGA
    GCCATCCGTA AACAGCTGGC AGCTTTCTTA GAAGGCTTCT ATGAGATCAT TCCAAAGCGC
    CTCATTTCTA TCTTCACTGA GCAGGAGTTA GAGCTGCTCA TATCAGGACT GCCCACAATC
    GACATTGATG ATTTGAAATC CAACACTGAA TACCACAAGT ACCAGTCCAA CTCTATTCAG
    ATCCAGTGGT TCTGGAGAGC ATTGCGTTCT TTCGACCAAG CTGACCGTGC CAAGTTCCTC
    CAATTTGTCA CGGGTACTTC AAAGGTGCCC TTACAAGGCT TTGCTGCCCT TGAAGGCATG
    AATGGCATTC AGAAGTTTCA GATCCATCGG GATGACAGGT CCACAGATCG CCTGCCTTCA
    GCTCACACAT GTTTTAATCA GCTGGACCTA CCTGCCTATG AGAGCTTTGA AAAGCTCCGC
    CACATGTTAC TATTGGCCAT CCAGGAGTGC TCTGAAGGCT TTGGGCTGGC CTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45534
    Clone ID Related Accession (Same CDS sequence) XM_014111527.1 , XM_014111527.2
    Accession Version XM_014111527.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1321
    1381
    1441
    1501
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    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG ATTCCATGAA TATCCTGGAC
    CCTGAGGATG AGGAGGAGCA CACTCAGGAA GAGGACAGCA GTGGCAGTAA CGAGGATGAG
    GATGATAGTC AGGATGAAGA GGAGGAGGAG GAGGAAGATG AGGAAGATGA TCAGGAGGAT
    GATGAAGGTG AAGAGGGAGA TGAAGACGAT GACGACGATG GCTCTGAAAT GGAATTGGAT
    GAGGATTACC CTGATATGAA CGCTTCTCCC TTGGTCCGAT TTGAGCGCTT TGACCGGGAG
    GATGATCTCA TCATTGAGTT TGACAACATG TTCTCCAGCG CTACAGACAT CCCCCCATCC
    CCAGGAAATA TCCCTACCAC TCACCCACTG ATGGTACGCC ATGCAGACCA CAGTTCTCTG
    ACACTGGGCA GTGGCTCCTC CACAACACGT CTCACCCAGG GCATCGGGCG CAGTCAGAGG
    ACCCTAAGGC AGCTGACAGC CAATACAGGC CACACCATTC ATGTTCATTA CCCTGGGAAC
    CGCCAGCCCA ACCCTCCTCT TATACTCCAG AGGTTGCTTG GTCCTTCTGC TGCTGCTGAC
    ATCCTTCAGC TGAGCAGCAG CCTTCCCCTA CAAAGCCGTG GACGAGCCCG CCTCCTGGTA
    GGCAATGATG ATGTCCACAT CATTGCCAGA TCTGATGATG AACTGCTGGA TGACTTTTTC
    CATGACCAGA GCACAGCTAC CAGCCAAGCA GGGACCCTAT CCAGCATCCC CACAGCCCTG
    ACCCGCTGGA CAGAGGAATG CAAAGTTCTT GATGCCGAGA GCATGCATGA CTGTGTTTCA
    GTTGTTAAAG TCCCCATTGT CAATCACTTG GAATTCCTGA GGGATGAGGA GCTGGAAGAA
    AGGCGGGAGA AACGCAGGAA ACAATTGGCT GAGGAAGAAA CAAAGATAAC TGACAAAGGC
    AAAGAAGATA AGGAGAACAG GGATCAGAGT GCACAGTGTA CTGCATCTAA AACAAATGAC
    TCCGCTGAAC AGAACCTCTC AGATGGGACT CCTATGCCTG ACAGCTACCC AACAACCCCA
    TCTTCAACTG ATGCAGCCAC ATCTGAGTCC AAGGATACCC TTGTCACTCT GCAGCCCTCA
    CAACAGCAAC AAACACTCCC ACCCCCACCA GCCTTGGGAG AGATTCCTCA AGAGCTACAG
    TCCCCGGCTG GAGAAGGGGG GAGCTCTACA CAGCTGTTGA TGCCTGTAGA GCCAGAGGAA
    TTGGGTCCCA CAAGGCCAAG TGGAGAAGCA GAAACAACTC AGATGGAGTT GTCCCCAGCT
    CCTACTATAA GCAGAGATCT AACCTCTCCA TGTGACTTCC TCCTAGCCTC TCTTTCCCCA
    GAGAGGGCTG AAGATTCTGA TGCGCTGACA GCTGTCAGCA GTCAACTGGA AGGCTCTCCC
    ATGGACACCA GTAGCCTGGC TTCCTGTACC CTAGAGGAGG CTGTGGGTGA CACCTCAGCA
    GCTGGCAGTT CTGAGCAGCC CACAGCAGGC AGCTCCACTC CTGGGGATGC CCCAACTGTT
    GTGACAGAAG TGCAAAGCAG GGGTGATGGG TCAGGTGAAC CTACCCAGCC CCCTGAAGAC
    AGCTCTCCCC CTGCATCCTC TGAGAGTTCT TCCACCAGAG ATTCTGCTGT GGCCATTTCT
    GGAGCAGATT CCCGAGGAAT CCTAGAAGAG CCACTGCCTT CAACAAGCAG TGAGGAAGAA
    GATCCCCTTG CAGGTATCAG TCTACCAGAA GGTGTGGACC CCTCTTTCCT GGCTGCCCTG
    CCTGATGACA TCCGCCGGGA AGTCCTACAG AACCAACTGG GCATCCGTCC ACCAACTCGG
    ACTGCCCCTT CGGCAAATAG CTCAGCTCCT GCAGTGGTGG GGAATCCTGG TGTGACCGAA
    GTGAGCCCTG AGTTCCTGGC GGCCCTGCCT CCAGCCATTC AGGAGGAAGT GCTGGCACAG
    CAGAGAGCTG AGCAACAACG ACGGGAACTG GCACAGAATG CGAGCTCAGA CACTCCCATG
    GACCCTGTGA CCTTCATCCA GACCCTGCCA TCAGACTTGC GCCGTAGTGT CCTGGAGGAC
    ATGGAGGACA GTGTTTTGGC TGTGATGCCA CCTGACATTG CAGCTGAGGC TCAAGCCCTG
    AGGCGAGAGC AGGAAGCCCG GCAGCGACAG CTCATGCATG AGCGGCTCTT TGGCCACAGC
    AGCACCTCTG CGCTCTCTGC CATCCTCCGA AGCCCGGCTT TCACCAGTCG CCTCAGTGGC
    AACCGAGGAG TCCAATACAC TCGTCTTGCT GTGCAAAGAG GTGGCACCTT CCAGATGGGG
    GGTAGCAGCA GCCATAACAG GCCTTCTGGC AGTAATGTGG ATACTCTTCT CCGCCTCCGA
    GGACGGCTCC TCCTGGACCA TGAAGCCCTA TCCTGTCTCC TGGTCCTACT TTTCGTGGAT
    GAGCCAAAGC TTAATACTAG TCGTCTACAC CGAGTACTGA GAAATCTCTG CTACCATGCC
    CAGACCCGCC ACTGGGTCAT CCGCAGTCTG CTCTCCATTT TGCAGCGCAG CAGTGAAAGT
    GAGCTATGCA TTGAAACACC CAAGCTCTCC TCAAGTGAGG ACAAGGGCAA AAAGTCAAGC
    AAAAGCTGTG GGTCAAGCAG CCATGAGAAC CGGCCCCTGG ACCTCCTACA CAAGATGGAG
    TCTAAGAGCT CCAACCAGCT TTCCTGGCTC TCAGTATCCA TGGATGCAGC CCTCGGCTGC
    AGGACTAATA TATTCCAGAT TCAACGTTCA GGAGGTCGCA AACATACCGA GAAGCATGCA
    AGCAGTGGCT CCACTGTCCA CATCCACCCC CAGGCTGCTC CTGTTGTCTG CAGACATGTT
    CTAGACACAC TCATTCAATT GGCCAAGGTA TTTCCCAGCC ATTTTACACA GCAGCGGACC
    AAAGAAACAA ACTGTGAGAG TGATCGGGAG AGGGGCAGTA AGCAGGCCTG CAGCCCATGC
    TCTTCACAGT CCACCAGCAG TGGGATTTGC ACAGACTTCT GGGACTTACT GGTTAAACTG
    GACAACATGA ATGTCAGCCG GAAAGGCAAG AACTCCGTGA AGTCAGTGCC AGTGAGCGCT
    GGTGGTGAGG GGGAAACCTC CCCATACAGC CTTGAGGCCT CTCCACTGGG GCAGCTCATG
    AACATGTTAT CACACCCAGT CATCCGCCGG AGCTCTCTCT TAACTGAGAA ACTCCTCAGA
    CTCCTTTCTC TCATCTCAAT TGCTCTCCCA GAAAACAAAG TGTCGGAAGC ACAGGCTAAT
    TCTGGCAGCA GTGCTTCCTC CACCACTGTT GCCACCTCAA CCACATCTAC CACCACTACC
    ACTGCTGCCT CCAGCACGCC CACTCCCCCT GCTGCAACCA CCCCTGTCAC TTCTGCTCCA
    GCTCTGGTTG CTGCCACGGC TATATCCACC ATTGCCGTAG CTGCTTCGAC CACGGTGACT
    ACCCCCACGA CTGCTACCAC TACTGTTTCA ACTTCTACAA CTACTAAGGC CAGCAAATCT
    CCAGCAAAGG TGGGTGATGG GGGCAGCAGC AGTGCTGACT TTAAGATGGT ATCCTCTGGC
    CTCACTGAAA ACCAGCTGCA GCTCTCTGTG GAGGTGTTGA CATCCCACTC TTGTTCCGAA
    GAAGGTCTAG AGGATGCAGC CAATGTGTTA CTGCAGCTCT CTCGGGGGGA CCCTGCAACC
    CGGGACACTG TTCTCAAGCT GCTACTGAAT GGAGCGCGCC ATCTGGGTTA TACTCTTTGT
    AAACAGATAG GTACCCTTCT GGCTGAGTTA CGGGAATACA ACCTAGAACA GCAGCGACGA
    GCCCAATGTG AGACCCTCTC TCCTGATGGC CTGCCTGAGG AGCAGCCACA GACCACCAAG
    CTAAAAGGCA AAATGCAGAG CAGGTTTGAC ATGGCTGAGA ATGTGGTAAT TGTGGCATCT
    CAGAAGCGAC CTCTGGGTGG CCGGGAGCTC CAGCTGCCTT CTATGTCCAT GTTGACATCC
    AAGACGTCTA CCCAGAAGTT CTTCTTGAGG GTACTGCAGG TCATCATCCA GCTTCGGGAC
    GACACACGCC GAGCTAACAA GAAAGCCAAG CAGACAGGCA GGCTAGGTTC TTCCGGTTTA
    GGCTCAGCTA GCAGCATCCA GGCAGCTGTT CGGCAGCTGG AGGCTGAGGC TGATGCCATT
    ATACAAATGG TACGTGAGGG TCAAAGGGCG CGGAGACAGC AACAAGCAGC AACGTCGGAG
    TCAAGCCAGT CAGAGGCCTC TGTCCGGAGA GAGGAGTCAC CCATGGATGT GGACCAGCCA
    TCTCCCAGTG CTCAGGATAC TCAGTCCATT GGCTCAGATG GAACCCCACA AGGGGAAAAG
    GAGAAGGAGG AGAGGCCACC TGAGTTACCC CTGCTCAGTG AGCAGCTGAG CTTGGACGAG
    CTGTGGGACA TGCTGGGAGA GTGTCTAAAG GAACTGGAGG AATCCCATGA CCAGCATGCA
    GTGCTAGTGC TACAGCCTGC CGTCGAGGCC TTCTTTTTGG TCCATGCCAC AGAGCGGGAG
    AGCAAACCTC CTGTCAGAGA CACCCGTGAG AGCCAGCTGG CACACATCAA GGACGAGCCT
    CCTCCACTCT CCCCTGCCCC CTTAACCCCT GCTACTCCTT CCTCCCTTGA CCCATTCTTC
    TCCAGGGAGC CCTCCTCTAT GCACATCTCC TCAAGCCTGC CCCCTGATAC ACAGAAGTTC
    CTTCGCTTTG CAGAGACTCA CCGCACTGTG TTAAATCAGA TCCTACGGCA GTCCACCACC
    CACCTCGCTG ATGGGCCTTT TGCTGTCCTG GTAGACTACA TTCGTGTCCT CGACTTTGAT
    GTCAAGCGCA AATATTTCCG CCAAGAGCTG GAACGTCTGG ATGAGGGGCT CCGGAAAGAA
    GACATGGCTG TGCACGTCCG CCGTGATCAT GTGTTTGAAG ACTCCTATCG TGAACTGCAC
    CGGAAATCCC CTGAAGAAAT GAAGAATCGA TTGTATATAG TGTTTGAAGG AGAAGAAGGG
    CAGGATGCTG GAGGGCTCCT GCGGGAGTGG TATATGATCA TCTCTCGGGA GATGTTTAAC
    CCTATGTATG CCTTGTTCCG TACCTCACCC GGTGATCGGG TCACCTACAC CATCAACCCA
    TCTTCCCACT GCAACCCCAA TCACCTCAGC TACTTCAAGT TTGTTGGACG CATTGTGGCC
    AAAGCTGTAT ATGACAACCG TCTCCTGGAG TGCTATTTTA CTCGGTCCTT CTACAAACAC
    ATCTTGGGCA AGTCTGTCAG ATATACAGAT ATGGAGAGTG AAGATTACCA CTTCTACCAG
    GGCCTGGTTT ATCTGCTGGA AAATGATGTC TCCACACTAG GTTATGACCT CACCTTCAGC
    ACTGAGGTCC AGGAGTTTGG AGTCTGTGAA GTTCGTGACC TCAAACCCAA TGGGGCTAAC
    ATCTTGGTAA CTGAAGAGAA TAAGAAGGAA TATGTACACC TGGTGTGCCA GATGAGGATG
    ACAGGAGCCA TCCGTAAACA GCTGGCAGCT TTCTTAGAAG GCTTCTATGA GATCATTCCA
    AAGCGCCTCA TTTCTATCTT CACTGAGCAG GAGTTAGAGC TGCTCATATC AGGACTGCCC
    ACAATCGACA TTGATGATTT GAAATCCAAC ACTGAATACC ACAAGTACCA GTCCAACTCT
    ATTCAGATCC AGTGGTTCTG GAGAGCATTG CGTTCTTTCG ACCAAGCTGA CCGTGCCAAG
    TTCCTCCAAT TTGTCACGGG TACTTCAAAG GTGCCCTTAC AAGGCTTTGC TGCCCTTGAA
    GGCATGAATG GCATTCAGAA GTTTCAGATC CATCGGGATG ACAGGTCCAC AGATCGCCTG
    CCTTCAGCTC ACACATGTTT TAATCAGCTG GACCTACCTG CCTATGAGAG CTTTGAAAAG
    CTCCGCCACA TGTTACTATT GGCCATCCAG GAGTGCTCTG AAGGCTTTGG GCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967002.1
    CDS452..13651
    Translation

    Target ORF information:

    RefSeq Version XM_014111527.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111527.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG ATTCCATGAA TATCCTGGAC
    CCTGAGGATG AGGAGGAGCA CACTCAGGAA GAGGACAGCA GTGGCAGTAA CGAGGATGAG
    GATGATAGTC AGGATGAAGA GGAGGAGGAG GAGGAAGATG AGGAAGATGA TCAGGAGGAT
    GATGAAGGTG AAGAGGGAGA TGAAGACGAT GACGACGATG GCTCTGAAAT GGAATTGGAT
    GAGGATTACC CTGATATGAA CGCTTCTCCC TTGGTCCGAT TTGAGCGCTT TGACCGGGAG
    GATGATCTCA TCATTGAGTT TGACAACATG TTCTCCAGCG CTACAGACAT CCCCCCATCC
    CCAGGAAATA TCCCTACCAC TCACCCACTG ATGGTACGCC ATGCAGACCA CAGTTCTCTG
    ACACTGGGCA GTGGCTCCTC CACAACACGT CTCACCCAGG GCATCGGGCG CAGTCAGAGG
    ACCCTAAGGC AGCTGACAGC CAATACAGGC CACACCATTC ATGTTCATTA CCCTGGGAAC
    CGCCAGCCCA ACCCTCCTCT TATACTCCAG AGGTTGCTTG GTCCTTCTGC TGCTGCTGAC
    ATCCTTCAGC TGAGCAGCAG CCTTCCCCTA CAAAGCCGTG GACGAGCCCG CCTCCTGGTA
    GGCAATGATG ATGTCCACAT CATTGCCAGA TCTGATGATG AACTGCTGGA TGACTTTTTC
    CATGACCAGA GCACAGCTAC CAGCCAAGCA GGGACCCTAT CCAGCATCCC CACAGCCCTG
    ACCCGCTGGA CAGAGGAATG CAAAGTTCTT GATGCCGAGA GCATGCATGA CTGTGTTTCA
    GTTGTTAAAG TCCCCATTGT CAATCACTTG GAATTCCTGA GGGATGAGGA GCTGGAAGAA
    AGGCGGGAGA AACGCAGGAA ACAATTGGCT GAGGAAGAAA CAAAGATAAC TGACAAAGGC
    AAAGAAGATA AGGAGAACAG GGATCAGAGT GCACAGTGTA CTGCATCTAA AACAAATGAC
    TCCGCTGAAC AGAACCTCTC AGATGGGACT CCTATGCCTG ACAGCTACCC AACAACCCCA
    TCTTCAACTG ATGCAGCCAC ATCTGAGTCC AAGGATACCC TTGTCACTCT GCAGCCCTCA
    CAACAGCAAC AAACACTCCC ACCCCCACCA GCCTTGGGAG AGATTCCTCA AGAGCTACAG
    TCCCCGGCTG GAGAAGGGGG GAGCTCTACA CAGCTGTTGA TGCCTGTAGA GCCAGAGGAA
    TTGGGTCCCA CAAGGCCAAG TGGAGAAGCA GAAACAACTC AGATGGAGTT GTCCCCAGCT
    CCTACTATAA GCAGAGATCT AACCTCTCCA TGTGACTTCC TCCTAGCCTC TCTTTCCCCA
    GAGAGGGCTG AAGATTCTGA TGCGCTGACA GCTGTCAGCA GTCAACTGGA AGGCTCTCCC
    ATGGACACCA GTAGCCTGGC TTCCTGTACC CTAGAGGAGG CTGTGGGTGA CACCTCAGCA
    GCTGGCAGTT CTGAGCAGCC CACAGCAGGC AGCTCCACTC CTGGGGATGC CCCAACTGTT
    GTGACAGAAG TGCAAAGCAG GGGTGATGGG TCAGGTGAAC CTACCCAGCC CCCTGAAGAC
    AGCTCTCCCC CTGCATCCTC TGAGAGTTCT TCCACCAGAG ATTCTGCTGT GGCCATTTCT
    GGAGCAGATT CCCGAGGAAT CCTAGAAGAG CCACTGCCTT CAACAAGCAG TGAGGAAGAA
    GATCCCCTTG CAGGTATCAG TCTACCAGAA GGTGTGGACC CCTCTTTCCT GGCTGCCCTG
    CCTGATGACA TCCGCCGGGA AGTCCTACAG AACCAACTGG GCATCCGTCC ACCAACTCGG
    ACTGCCCCTT CGGCAAATAG CTCAGCTCCT GCAGTGGTGG GGAATCCTGG TGTGACCGAA
    GTGAGCCCTG AGTTCCTGGC GGCCCTGCCT CCAGCCATTC AGGAGGAAGT GCTGGCACAG
    CAGAGAGCTG AGCAACAACG ACGGGAACTG GCACAGAATG CGAGCTCAGA CACTCCCATG
    GACCCTGTGA CCTTCATCCA GACCCTGCCA TCAGACTTGC GCCGTAGTGT CCTGGAGGAC
    ATGGAGGACA GTGTTTTGGC TGTGATGCCA CCTGACATTG CAGCTGAGGC TCAAGCCCTG
    AGGCGAGAGC AGGAAGCCCG GCAGCGACAG CTCATGCATG AGCGGCTCTT TGGCCACAGC
    AGCACCTCTG CGCTCTCTGC CATCCTCCGA AGCCCGGCTT TCACCAGTCG CCTCAGTGGC
    AACCGAGGAG TCCAATACAC TCGTCTTGCT GTGCAAAGAG GTGGCACCTT CCAGATGGGG
    GGTAGCAGCA GCCATAACAG GCCTTCTGGC AGTAATGTGG ATACTCTTCT CCGCCTCCGA
    GGACGGCTCC TCCTGGACCA TGAAGCCCTA TCCTGTCTCC TGGTCCTACT TTTCGTGGAT
    GAGCCAAAGC TTAATACTAG TCGTCTACAC CGAGTACTGA GAAATCTCTG CTACCATGCC
    CAGACCCGCC ACTGGGTCAT CCGCAGTCTG CTCTCCATTT TGCAGCGCAG CAGTGAAAGT
    GAGCTATGCA TTGAAACACC CAAGCTCTCC TCAAGTGAGG ACAAGGGCAA AAAGTCAAGC
    AAAAGCTGTG GGTCAAGCAG CCATGAGAAC CGGCCCCTGG ACCTCCTACA CAAGATGGAG
    TCTAAGAGCT CCAACCAGCT TTCCTGGCTC TCAGTATCCA TGGATGCAGC CCTCGGCTGC
    AGGACTAATA TATTCCAGAT TCAACGTTCA GGAGGTCGCA AACATACCGA GAAGCATGCA
    AGCAGTGGCT CCACTGTCCA CATCCACCCC CAGGCTGCTC CTGTTGTCTG CAGACATGTT
    CTAGACACAC TCATTCAATT GGCCAAGGTA TTTCCCAGCC ATTTTACACA GCAGCGGACC
    AAAGAAACAA ACTGTGAGAG TGATCGGGAG AGGGGCAGTA AGCAGGCCTG CAGCCCATGC
    TCTTCACAGT CCACCAGCAG TGGGATTTGC ACAGACTTCT GGGACTTACT GGTTAAACTG
    GACAACATGA ATGTCAGCCG GAAAGGCAAG AACTCCGTGA AGTCAGTGCC AGTGAGCGCT
    GGTGGTGAGG GGGAAACCTC CCCATACAGC CTTGAGGCCT CTCCACTGGG GCAGCTCATG
    AACATGTTAT CACACCCAGT CATCCGCCGG AGCTCTCTCT TAACTGAGAA ACTCCTCAGA
    CTCCTTTCTC TCATCTCAAT TGCTCTCCCA GAAAACAAAG TGTCGGAAGC ACAGGCTAAT
    TCTGGCAGCA GTGCTTCCTC CACCACTGTT GCCACCTCAA CCACATCTAC CACCACTACC
    ACTGCTGCCT CCAGCACGCC CACTCCCCCT GCTGCAACCA CCCCTGTCAC TTCTGCTCCA
    GCTCTGGTTG CTGCCACGGC TATATCCACC ATTGCCGTAG CTGCTTCGAC CACGGTGACT
    ACCCCCACGA CTGCTACCAC TACTGTTTCA ACTTCTACAA CTACTAAGGC CAGCAAATCT
    CCAGCAAAGG TGGGTGATGG GGGCAGCAGC AGTGCTGACT TTAAGATGGT ATCCTCTGGC
    CTCACTGAAA ACCAGCTGCA GCTCTCTGTG GAGGTGTTGA CATCCCACTC TTGTTCCGAA
    GAAGGTCTAG AGGATGCAGC CAATGTGTTA CTGCAGCTCT CTCGGGGGGA CCCTGCAACC
    CGGGACACTG TTCTCAAGCT GCTACTGAAT GGAGCGCGCC ATCTGGGTTA TACTCTTTGT
    AAACAGATAG GTACCCTTCT GGCTGAGTTA CGGGAATACA ACCTAGAACA GCAGCGACGA
    GCCCAATGTG AGACCCTCTC TCCTGATGGC CTGCCTGAGG AGCAGCCACA GACCACCAAG
    CTAAAAGGCA AAATGCAGAG CAGGTTTGAC ATGGCTGAGA ATGTGGTAAT TGTGGCATCT
    CAGAAGCGAC CTCTGGGTGG CCGGGAGCTC CAGCTGCCTT CTATGTCCAT GTTGACATCC
    AAGACGTCTA CCCAGAAGTT CTTCTTGAGG GTACTGCAGG TCATCATCCA GCTTCGGGAC
    GACACACGCC GAGCTAACAA GAAAGCCAAG CAGACAGGCA GGCTAGGTTC TTCCGGTTTA
    GGCTCAGCTA GCAGCATCCA GGCAGCTGTT CGGCAGCTGG AGGCTGAGGC TGATGCCATT
    ATACAAATGG TACGTGAGGG TCAAAGGGCG CGGAGACAGC AACAAGCAGC AACGTCGGAG
    TCAAGCCAGT CAGAGGCCTC TGTCCGGAGA GAGGAGTCAC CCATGGATGT GGACCAGCCA
    TCTCCCAGTG CTCAGGATAC TCAGTCCATT GGCTCAGATG GAACCCCACA AGGGGAAAAG
    GAGAAGGAGG AGAGGCCACC TGAGTTACCC CTGCTCAGTG AGCAGCTGAG CTTGGACGAG
    CTGTGGGACA TGCTGGGAGA GTGTCTAAAG GAACTGGAGG AATCCCATGA CCAGCATGCA
    GTGCTAGTGC TACAGCCTGC CGTCGAGGCC TTCTTTTTGG TCCATGCCAC AGAGCGGGAG
    AGCAAACCTC CTGTCAGAGA CACCCGTGAG AGCCAGCTGG CACACATCAA GGACGAGCCT
    CCTCCACTCT CCCCTGCCCC CTTAACCCCT GCTACTCCTT CCTCCCTTGA CCCATTCTTC
    TCCAGGGAGC CCTCCTCTAT GCACATCTCC TCAAGCCTGC CCCCTGATAC ACAGAAGTTC
    CTTCGCTTTG CAGAGACTCA CCGCACTGTG TTAAATCAGA TCCTACGGCA GTCCACCACC
    CACCTCGCTG ATGGGCCTTT TGCTGTCCTG GTAGACTACA TTCGTGTCCT CGACTTTGAT
    GTCAAGCGCA AATATTTCCG CCAAGAGCTG GAACGTCTGG ATGAGGGGCT CCGGAAAGAA
    GACATGGCTG TGCACGTCCG CCGTGATCAT GTGTTTGAAG ACTCCTATCG TGAACTGCAC
    CGGAAATCCC CTGAAGAAAT GAAGAATCGA TTGTATATAG TGTTTGAAGG AGAAGAAGGG
    CAGGATGCTG GAGGGCTCCT GCGGGAGTGG TATATGATCA TCTCTCGGGA GATGTTTAAC
    CCTATGTATG CCTTGTTCCG TACCTCACCC GGTGATCGGG TCACCTACAC CATCAACCCA
    TCTTCCCACT GCAACCCCAA TCACCTCAGC TACTTCAAGT TTGTTGGACG CATTGTGGCC
    AAAGCTGTAT ATGACAACCG TCTCCTGGAG TGCTATTTTA CTCGGTCCTT CTACAAACAC
    ATCTTGGGCA AGTCTGTCAG ATATACAGAT ATGGAGAGTG AAGATTACCA CTTCTACCAG
    GGCCTGGTTT ATCTGCTGGA AAATGATGTC TCCACACTAG GTTATGACCT CACCTTCAGC
    ACTGAGGTCC AGGAGTTTGG AGTCTGTGAA GTTCGTGACC TCAAACCCAA TGGGGCTAAC
    ATCTTGGTAA CTGAAGAGAA TAAGAAGGAA TATGTACACC TGGTGTGCCA GATGAGGATG
    ACAGGAGCCA TCCGTAAACA GCTGGCAGCT TTCTTAGAAG GCTTCTATGA GATCATTCCA
    AAGCGCCTCA TTTCTATCTT CACTGAGCAG GAGTTAGAGC TGCTCATATC AGGACTGCCC
    ACAATCGACA TTGATGATTT GAAATCCAAC ACTGAATACC ACAAGTACCA GTCCAACTCT
    ATTCAGATCC AGTGGTTCTG GAGAGCATTG CGTTCTTTCG ACCAAGCTGA CCGTGCCAAG
    TTCCTCCAAT TTGTCACGGG TACTTCAAAG GTGCCCTTAC AAGGCTTTGC TGCCCTTGAA
    GGCATGAATG GCATTCAGAA GTTTCAGATC CATCGGGATG ACAGGTCCAC AGATCGCCTG
    CCTTCAGCTC ACACATGTTT TAATCAGCTG GACCTACCTG CCTATGAGAG CTTTGAAAAG
    CTCCGCCACA TGTTACTATT GGCCATCCAG GAGTGCTCTG AAGGCTTTGG GCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45534
    Clone ID Related Accession (Same CDS sequence) XM_014111527.1 , XM_014111527.2
    Accession Version XM_014111527.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13200bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111527.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
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    2581
    2641
    2701
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    9781
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    10501
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    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG ATTCCATGAA TATCCTGGAC
    CCTGAGGATG AGGAGGAGCA CACTCAGGAA GAGGACAGCA GTGGCAGTAA CGAGGATGAG
    GATGATAGTC AGGATGAAGA GGAGGAGGAG GAGGAAGATG AGGAAGATGA TCAGGAGGAT
    GATGAAGGTG AAGAGGGAGA TGAAGACGAT GACGACGATG GCTCTGAAAT GGAATTGGAT
    GAGGATTACC CTGATATGAA CGCTTCTCCC TTGGTCCGAT TTGAGCGCTT TGACCGGGAG
    GATGATCTCA TCATTGAGTT TGACAACATG TTCTCCAGCG CTACAGACAT CCCCCCATCC
    CCAGGAAATA TCCCTACCAC TCACCCACTG ATGGTACGCC ATGCAGACCA CAGTTCTCTG
    ACACTGGGCA GTGGCTCCTC CACAACACGT CTCACCCAGG GCATCGGGCG CAGTCAGAGG
    ACCCTAAGGC AGCTGACAGC CAATACAGGC CACACCATTC ATGTTCATTA CCCTGGGAAC
    CGCCAGCCCA ACCCTCCTCT TATACTCCAG AGGTTGCTTG GTCCTTCTGC TGCTGCTGAC
    ATCCTTCAGC TGAGCAGCAG CCTTCCCCTA CAAAGCCGTG GACGAGCCCG CCTCCTGGTA
    GGCAATGATG ATGTCCACAT CATTGCCAGA TCTGATGATG AACTGCTGGA TGACTTTTTC
    CATGACCAGA GCACAGCTAC CAGCCAAGCA GGGACCCTAT CCAGCATCCC CACAGCCCTG
    ACCCGCTGGA CAGAGGAATG CAAAGTTCTT GATGCCGAGA GCATGCATGA CTGTGTTTCA
    GTTGTTAAAG TCCCCATTGT CAATCACTTG GAATTCCTGA GGGATGAGGA GCTGGAAGAA
    AGGCGGGAGA AACGCAGGAA ACAATTGGCT GAGGAAGAAA CAAAGATAAC TGACAAAGGC
    AAAGAAGATA AGGAGAACAG GGATCAGAGT GCACAGTGTA CTGCATCTAA AACAAATGAC
    TCCGCTGAAC AGAACCTCTC AGATGGGACT CCTATGCCTG ACAGCTACCC AACAACCCCA
    TCTTCAACTG ATGCAGCCAC ATCTGAGTCC AAGGATACCC TTGTCACTCT GCAGCCCTCA
    CAACAGCAAC AAACACTCCC ACCCCCACCA GCCTTGGGAG AGATTCCTCA AGAGCTACAG
    TCCCCGGCTG GAGAAGGGGG GAGCTCTACA CAGCTGTTGA TGCCTGTAGA GCCAGAGGAA
    TTGGGTCCCA CAAGGCCAAG TGGAGAAGCA GAAACAACTC AGATGGAGTT GTCCCCAGCT
    CCTACTATAA GCAGAGATCT AACCTCTCCA TGTGACTTCC TCCTAGCCTC TCTTTCCCCA
    GAGAGGGCTG AAGATTCTGA TGCGCTGACA GCTGTCAGCA GTCAACTGGA AGGCTCTCCC
    ATGGACACCA GTAGCCTGGC TTCCTGTACC CTAGAGGAGG CTGTGGGTGA CACCTCAGCA
    GCTGGCAGTT CTGAGCAGCC CACAGCAGGC AGCTCCACTC CTGGGGATGC CCCAACTGTT
    GTGACAGAAG TGCAAAGCAG GGGTGATGGG TCAGGTGAAC CTACCCAGCC CCCTGAAGAC
    AGCTCTCCCC CTGCATCCTC TGAGAGTTCT TCCACCAGAG ATTCTGCTGT GGCCATTTCT
    GGAGCAGATT CCCGAGGAAT CCTAGAAGAG CCACTGCCTT CAACAAGCAG TGAGGAAGAA
    GATCCCCTTG CAGGTATCAG TCTACCAGAA GGTGTGGACC CCTCTTTCCT GGCTGCCCTG
    CCTGATGACA TCCGCCGGGA AGTCCTACAG AACCAACTGG GCATCCGTCC ACCAACTCGG
    ACTGCCCCTT CGGCAAATAG CTCAGCTCCT GCAGTGGTGG GGAATCCTGG TGTGACCGAA
    GTGAGCCCTG AGTTCCTGGC GGCCCTGCCT CCAGCCATTC AGGAGGAAGT GCTGGCACAG
    CAGAGAGCTG AGCAACAACG ACGGGAACTG GCACAGAATG CGAGCTCAGA CACTCCCATG
    GACCCTGTGA CCTTCATCCA GACCCTGCCA TCAGACTTGC GCCGTAGTGT CCTGGAGGAC
    ATGGAGGACA GTGTTTTGGC TGTGATGCCA CCTGACATTG CAGCTGAGGC TCAAGCCCTG
    AGGCGAGAGC AGGAAGCCCG GCAGCGACAG CTCATGCATG AGCGGCTCTT TGGCCACAGC
    AGCACCTCTG CGCTCTCTGC CATCCTCCGA AGCCCGGCTT TCACCAGTCG CCTCAGTGGC
    AACCGAGGAG TCCAATACAC TCGTCTTGCT GTGCAAAGAG GTGGCACCTT CCAGATGGGG
    GGTAGCAGCA GCCATAACAG GCCTTCTGGC AGTAATGTGG ATACTCTTCT CCGCCTCCGA
    GGACGGCTCC TCCTGGACCA TGAAGCCCTA TCCTGTCTCC TGGTCCTACT TTTCGTGGAT
    GAGCCAAAGC TTAATACTAG TCGTCTACAC CGAGTACTGA GAAATCTCTG CTACCATGCC
    CAGACCCGCC ACTGGGTCAT CCGCAGTCTG CTCTCCATTT TGCAGCGCAG CAGTGAAAGT
    GAGCTATGCA TTGAAACACC CAAGCTCTCC TCAAGTGAGG ACAAGGGCAA AAAGTCAAGC
    AAAAGCTGTG GGTCAAGCAG CCATGAGAAC CGGCCCCTGG ACCTCCTACA CAAGATGGAG
    TCTAAGAGCT CCAACCAGCT TTCCTGGCTC TCAGTATCCA TGGATGCAGC CCTCGGCTGC
    AGGACTAATA TATTCCAGAT TCAACGTTCA GGAGGTCGCA AACATACCGA GAAGCATGCA
    AGCAGTGGCT CCACTGTCCA CATCCACCCC CAGGCTGCTC CTGTTGTCTG CAGACATGTT
    CTAGACACAC TCATTCAATT GGCCAAGGTA TTTCCCAGCC ATTTTACACA GCAGCGGACC
    AAAGAAACAA ACTGTGAGAG TGATCGGGAG AGGGGCAGTA AGCAGGCCTG CAGCCCATGC
    TCTTCACAGT CCACCAGCAG TGGGATTTGC ACAGACTTCT GGGACTTACT GGTTAAACTG
    GACAACATGA ATGTCAGCCG GAAAGGCAAG AACTCCGTGA AGTCAGTGCC AGTGAGCGCT
    GGTGGTGAGG GGGAAACCTC CCCATACAGC CTTGAGGCCT CTCCACTGGG GCAGCTCATG
    AACATGTTAT CACACCCAGT CATCCGCCGG AGCTCTCTCT TAACTGAGAA ACTCCTCAGA
    CTCCTTTCTC TCATCTCAAT TGCTCTCCCA GAAAACAAAG TGTCGGAAGC ACAGGCTAAT
    TCTGGCAGCA GTGCTTCCTC CACCACTGTT GCCACCTCAA CCACATCTAC CACCACTACC
    ACTGCTGCCT CCAGCACGCC CACTCCCCCT GCTGCAACCA CCCCTGTCAC TTCTGCTCCA
    GCTCTGGTTG CTGCCACGGC TATATCCACC ATTGCCGTAG CTGCTTCGAC CACGGTGACT
    ACCCCCACGA CTGCTACCAC TACTGTTTCA ACTTCTACAA CTACTAAGGC CAGCAAATCT
    CCAGCAAAGG TGGGTGATGG GGGCAGCAGC AGTGCTGACT TTAAGATGGT ATCCTCTGGC
    CTCACTGAAA ACCAGCTGCA GCTCTCTGTG GAGGTGTTGA CATCCCACTC TTGTTCCGAA
    GAAGGTCTAG AGGATGCAGC CAATGTGTTA CTGCAGCTCT CTCGGGGGGA CCCTGCAACC
    CGGGACACTG TTCTCAAGCT GCTACTGAAT GGAGCGCGCC ATCTGGGTTA TACTCTTTGT
    AAACAGATAG GTACCCTTCT GGCTGAGTTA CGGGAATACA ACCTAGAACA GCAGCGACGA
    GCCCAATGTG AGACCCTCTC TCCTGATGGC CTGCCTGAGG AGCAGCCACA GACCACCAAG
    CTAAAAGGCA AAATGCAGAG CAGGTTTGAC ATGGCTGAGA ATGTGGTAAT TGTGGCATCT
    CAGAAGCGAC CTCTGGGTGG CCGGGAGCTC CAGCTGCCTT CTATGTCCAT GTTGACATCC
    AAGACGTCTA CCCAGAAGTT CTTCTTGAGG GTACTGCAGG TCATCATCCA GCTTCGGGAC
    GACACACGCC GAGCTAACAA GAAAGCCAAG CAGACAGGCA GGCTAGGTTC TTCCGGTTTA
    GGCTCAGCTA GCAGCATCCA GGCAGCTGTT CGGCAGCTGG AGGCTGAGGC TGATGCCATT
    ATACAAATGG TACGTGAGGG TCAAAGGGCG CGGAGACAGC AACAAGCAGC AACGTCGGAG
    TCAAGCCAGT CAGAGGCCTC TGTCCGGAGA GAGGAGTCAC CCATGGATGT GGACCAGCCA
    TCTCCCAGTG CTCAGGATAC TCAGTCCATT GGCTCAGATG GAACCCCACA AGGGGAAAAG
    GAGAAGGAGG AGAGGCCACC TGAGTTACCC CTGCTCAGTG AGCAGCTGAG CTTGGACGAG
    CTGTGGGACA TGCTGGGAGA GTGTCTAAAG GAACTGGAGG AATCCCATGA CCAGCATGCA
    GTGCTAGTGC TACAGCCTGC CGTCGAGGCC TTCTTTTTGG TCCATGCCAC AGAGCGGGAG
    AGCAAACCTC CTGTCAGAGA CACCCGTGAG AGCCAGCTGG CACACATCAA GGACGAGCCT
    CCTCCACTCT CCCCTGCCCC CTTAACCCCT GCTACTCCTT CCTCCCTTGA CCCATTCTTC
    TCCAGGGAGC CCTCCTCTAT GCACATCTCC TCAAGCCTGC CCCCTGATAC ACAGAAGTTC
    CTTCGCTTTG CAGAGACTCA CCGCACTGTG TTAAATCAGA TCCTACGGCA GTCCACCACC
    CACCTCGCTG ATGGGCCTTT TGCTGTCCTG GTAGACTACA TTCGTGTCCT CGACTTTGAT
    GTCAAGCGCA AATATTTCCG CCAAGAGCTG GAACGTCTGG ATGAGGGGCT CCGGAAAGAA
    GACATGGCTG TGCACGTCCG CCGTGATCAT GTGTTTGAAG ACTCCTATCG TGAACTGCAC
    CGGAAATCCC CTGAAGAAAT GAAGAATCGA TTGTATATAG TGTTTGAAGG AGAAGAAGGG
    CAGGATGCTG GAGGGCTCCT GCGGGAGTGG TATATGATCA TCTCTCGGGA GATGTTTAAC
    CCTATGTATG CCTTGTTCCG TACCTCACCC GGTGATCGGG TCACCTACAC CATCAACCCA
    TCTTCCCACT GCAACCCCAA TCACCTCAGC TACTTCAAGT TTGTTGGACG CATTGTGGCC
    AAAGCTGTAT ATGACAACCG TCTCCTGGAG TGCTATTTTA CTCGGTCCTT CTACAAACAC
    ATCTTGGGCA AGTCTGTCAG ATATACAGAT ATGGAGAGTG AAGATTACCA CTTCTACCAG
    GGCCTGGTTT ATCTGCTGGA AAATGATGTC TCCACACTAG GTTATGACCT CACCTTCAGC
    ACTGAGGTCC AGGAGTTTGG AGTCTGTGAA GTTCGTGACC TCAAACCCAA TGGGGCTAAC
    ATCTTGGTAA CTGAAGAGAA TAAGAAGGAA TATGTACACC TGGTGTGCCA GATGAGGATG
    ACAGGAGCCA TCCGTAAACA GCTGGCAGCT TTCTTAGAAG GCTTCTATGA GATCATTCCA
    AAGCGCCTCA TTTCTATCTT CACTGAGCAG GAGTTAGAGC TGCTCATATC AGGACTGCCC
    ACAATCGACA TTGATGATTT GAAATCCAAC ACTGAATACC ACAAGTACCA GTCCAACTCT
    ATTCAGATCC AGTGGTTCTG GAGAGCATTG CGTTCTTTCG ACCAAGCTGA CCGTGCCAAG
    TTCCTCCAAT TTGTCACGGG TACTTCAAAG GTGCCCTTAC AAGGCTTTGC TGCCCTTGAA
    GGCATGAATG GCATTCAGAA GTTTCAGATC CATCGGGATG ACAGGTCCAC AGATCGCCTG
    CCTTCAGCTC ACACATGTTT TAATCAGCTG GACCTACCTG CCTATGAGAG CTTTGAAAAG
    CTCCGCCACA TGTTACTATT GGCCATCCAG GAGTGCTCTG AAGGCTTTGG GCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967002.1
    CDS361..13560
    Translation

    Target ORF information:

    RefSeq Version XM_014111527.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111527.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG ATTCCATGAA TATCCTGGAC
    CCTGAGGATG AGGAGGAGCA CACTCAGGAA GAGGACAGCA GTGGCAGTAA CGAGGATGAG
    GATGATAGTC AGGATGAAGA GGAGGAGGAG GAGGAAGATG AGGAAGATGA TCAGGAGGAT
    GATGAAGGTG AAGAGGGAGA TGAAGACGAT GACGACGATG GCTCTGAAAT GGAATTGGAT
    GAGGATTACC CTGATATGAA CGCTTCTCCC TTGGTCCGAT TTGAGCGCTT TGACCGGGAG
    GATGATCTCA TCATTGAGTT TGACAACATG TTCTCCAGCG CTACAGACAT CCCCCCATCC
    CCAGGAAATA TCCCTACCAC TCACCCACTG ATGGTACGCC ATGCAGACCA CAGTTCTCTG
    ACACTGGGCA GTGGCTCCTC CACAACACGT CTCACCCAGG GCATCGGGCG CAGTCAGAGG
    ACCCTAAGGC AGCTGACAGC CAATACAGGC CACACCATTC ATGTTCATTA CCCTGGGAAC
    CGCCAGCCCA ACCCTCCTCT TATACTCCAG AGGTTGCTTG GTCCTTCTGC TGCTGCTGAC
    ATCCTTCAGC TGAGCAGCAG CCTTCCCCTA CAAAGCCGTG GACGAGCCCG CCTCCTGGTA
    GGCAATGATG ATGTCCACAT CATTGCCAGA TCTGATGATG AACTGCTGGA TGACTTTTTC
    CATGACCAGA GCACAGCTAC CAGCCAAGCA GGGACCCTAT CCAGCATCCC CACAGCCCTG
    ACCCGCTGGA CAGAGGAATG CAAAGTTCTT GATGCCGAGA GCATGCATGA CTGTGTTTCA
    GTTGTTAAAG TCCCCATTGT CAATCACTTG GAATTCCTGA GGGATGAGGA GCTGGAAGAA
    AGGCGGGAGA AACGCAGGAA ACAATTGGCT GAGGAAGAAA CAAAGATAAC TGACAAAGGC
    AAAGAAGATA AGGAGAACAG GGATCAGAGT GCACAGTGTA CTGCATCTAA AACAAATGAC
    TCCGCTGAAC AGAACCTCTC AGATGGGACT CCTATGCCTG ACAGCTACCC AACAACCCCA
    TCTTCAACTG ATGCAGCCAC ATCTGAGTCC AAGGATACCC TTGTCACTCT GCAGCCCTCA
    CAACAGCAAC AAACACTCCC ACCCCCACCA GCCTTGGGAG AGATTCCTCA AGAGCTACAG
    TCCCCGGCTG GAGAAGGGGG GAGCTCTACA CAGCTGTTGA TGCCTGTAGA GCCAGAGGAA
    TTGGGTCCCA CAAGGCCAAG TGGAGAAGCA GAAACAACTC AGATGGAGTT GTCCCCAGCT
    CCTACTATAA GCAGAGATCT AACCTCTCCA TGTGACTTCC TCCTAGCCTC TCTTTCCCCA
    GAGAGGGCTG AAGATTCTGA TGCGCTGACA GCTGTCAGCA GTCAACTGGA AGGCTCTCCC
    ATGGACACCA GTAGCCTGGC TTCCTGTACC CTAGAGGAGG CTGTGGGTGA CACCTCAGCA
    GCTGGCAGTT CTGAGCAGCC CACAGCAGGC AGCTCCACTC CTGGGGATGC CCCAACTGTT
    GTGACAGAAG TGCAAAGCAG GGGTGATGGG TCAGGTGAAC CTACCCAGCC CCCTGAAGAC
    AGCTCTCCCC CTGCATCCTC TGAGAGTTCT TCCACCAGAG ATTCTGCTGT GGCCATTTCT
    GGAGCAGATT CCCGAGGAAT CCTAGAAGAG CCACTGCCTT CAACAAGCAG TGAGGAAGAA
    GATCCCCTTG CAGGTATCAG TCTACCAGAA GGTGTGGACC CCTCTTTCCT GGCTGCCCTG
    CCTGATGACA TCCGCCGGGA AGTCCTACAG AACCAACTGG GCATCCGTCC ACCAACTCGG
    ACTGCCCCTT CGGCAAATAG CTCAGCTCCT GCAGTGGTGG GGAATCCTGG TGTGACCGAA
    GTGAGCCCTG AGTTCCTGGC GGCCCTGCCT CCAGCCATTC AGGAGGAAGT GCTGGCACAG
    CAGAGAGCTG AGCAACAACG ACGGGAACTG GCACAGAATG CGAGCTCAGA CACTCCCATG
    GACCCTGTGA CCTTCATCCA GACCCTGCCA TCAGACTTGC GCCGTAGTGT CCTGGAGGAC
    ATGGAGGACA GTGTTTTGGC TGTGATGCCA CCTGACATTG CAGCTGAGGC TCAAGCCCTG
    AGGCGAGAGC AGGAAGCCCG GCAGCGACAG CTCATGCATG AGCGGCTCTT TGGCCACAGC
    AGCACCTCTG CGCTCTCTGC CATCCTCCGA AGCCCGGCTT TCACCAGTCG CCTCAGTGGC
    AACCGAGGAG TCCAATACAC TCGTCTTGCT GTGCAAAGAG GTGGCACCTT CCAGATGGGG
    GGTAGCAGCA GCCATAACAG GCCTTCTGGC AGTAATGTGG ATACTCTTCT CCGCCTCCGA
    GGACGGCTCC TCCTGGACCA TGAAGCCCTA TCCTGTCTCC TGGTCCTACT TTTCGTGGAT
    GAGCCAAAGC TTAATACTAG TCGTCTACAC CGAGTACTGA GAAATCTCTG CTACCATGCC
    CAGACCCGCC ACTGGGTCAT CCGCAGTCTG CTCTCCATTT TGCAGCGCAG CAGTGAAAGT
    GAGCTATGCA TTGAAACACC CAAGCTCTCC TCAAGTGAGG ACAAGGGCAA AAAGTCAAGC
    AAAAGCTGTG GGTCAAGCAG CCATGAGAAC CGGCCCCTGG ACCTCCTACA CAAGATGGAG
    TCTAAGAGCT CCAACCAGCT TTCCTGGCTC TCAGTATCCA TGGATGCAGC CCTCGGCTGC
    AGGACTAATA TATTCCAGAT TCAACGTTCA GGAGGTCGCA AACATACCGA GAAGCATGCA
    AGCAGTGGCT CCACTGTCCA CATCCACCCC CAGGCTGCTC CTGTTGTCTG CAGACATGTT
    CTAGACACAC TCATTCAATT GGCCAAGGTA TTTCCCAGCC ATTTTACACA GCAGCGGACC
    AAAGAAACAA ACTGTGAGAG TGATCGGGAG AGGGGCAGTA AGCAGGCCTG CAGCCCATGC
    TCTTCACAGT CCACCAGCAG TGGGATTTGC ACAGACTTCT GGGACTTACT GGTTAAACTG
    GACAACATGA ATGTCAGCCG GAAAGGCAAG AACTCCGTGA AGTCAGTGCC AGTGAGCGCT
    GGTGGTGAGG GGGAAACCTC CCCATACAGC CTTGAGGCCT CTCCACTGGG GCAGCTCATG
    AACATGTTAT CACACCCAGT CATCCGCCGG AGCTCTCTCT TAACTGAGAA ACTCCTCAGA
    CTCCTTTCTC TCATCTCAAT TGCTCTCCCA GAAAACAAAG TGTCGGAAGC ACAGGCTAAT
    TCTGGCAGCA GTGCTTCCTC CACCACTGTT GCCACCTCAA CCACATCTAC CACCACTACC
    ACTGCTGCCT CCAGCACGCC CACTCCCCCT GCTGCAACCA CCCCTGTCAC TTCTGCTCCA
    GCTCTGGTTG CTGCCACGGC TATATCCACC ATTGCCGTAG CTGCTTCGAC CACGGTGACT
    ACCCCCACGA CTGCTACCAC TACTGTTTCA ACTTCTACAA CTACTAAGGC CAGCAAATCT
    CCAGCAAAGG TGGGTGATGG GGGCAGCAGC AGTGCTGACT TTAAGATGGT ATCCTCTGGC
    CTCACTGAAA ACCAGCTGCA GCTCTCTGTG GAGGTGTTGA CATCCCACTC TTGTTCCGAA
    GAAGGTCTAG AGGATGCAGC CAATGTGTTA CTGCAGCTCT CTCGGGGGGA CCCTGCAACC
    CGGGACACTG TTCTCAAGCT GCTACTGAAT GGAGCGCGCC ATCTGGGTTA TACTCTTTGT
    AAACAGATAG GTACCCTTCT GGCTGAGTTA CGGGAATACA ACCTAGAACA GCAGCGACGA
    GCCCAATGTG AGACCCTCTC TCCTGATGGC CTGCCTGAGG AGCAGCCACA GACCACCAAG
    CTAAAAGGCA AAATGCAGAG CAGGTTTGAC ATGGCTGAGA ATGTGGTAAT TGTGGCATCT
    CAGAAGCGAC CTCTGGGTGG CCGGGAGCTC CAGCTGCCTT CTATGTCCAT GTTGACATCC
    AAGACGTCTA CCCAGAAGTT CTTCTTGAGG GTACTGCAGG TCATCATCCA GCTTCGGGAC
    GACACACGCC GAGCTAACAA GAAAGCCAAG CAGACAGGCA GGCTAGGTTC TTCCGGTTTA
    GGCTCAGCTA GCAGCATCCA GGCAGCTGTT CGGCAGCTGG AGGCTGAGGC TGATGCCATT
    ATACAAATGG TACGTGAGGG TCAAAGGGCG CGGAGACAGC AACAAGCAGC AACGTCGGAG
    TCAAGCCAGT CAGAGGCCTC TGTCCGGAGA GAGGAGTCAC CCATGGATGT GGACCAGCCA
    TCTCCCAGTG CTCAGGATAC TCAGTCCATT GGCTCAGATG GAACCCCACA AGGGGAAAAG
    GAGAAGGAGG AGAGGCCACC TGAGTTACCC CTGCTCAGTG AGCAGCTGAG CTTGGACGAG
    CTGTGGGACA TGCTGGGAGA GTGTCTAAAG GAACTGGAGG AATCCCATGA CCAGCATGCA
    GTGCTAGTGC TACAGCCTGC CGTCGAGGCC TTCTTTTTGG TCCATGCCAC AGAGCGGGAG
    AGCAAACCTC CTGTCAGAGA CACCCGTGAG AGCCAGCTGG CACACATCAA GGACGAGCCT
    CCTCCACTCT CCCCTGCCCC CTTAACCCCT GCTACTCCTT CCTCCCTTGA CCCATTCTTC
    TCCAGGGAGC CCTCCTCTAT GCACATCTCC TCAAGCCTGC CCCCTGATAC ACAGAAGTTC
    CTTCGCTTTG CAGAGACTCA CCGCACTGTG TTAAATCAGA TCCTACGGCA GTCCACCACC
    CACCTCGCTG ATGGGCCTTT TGCTGTCCTG GTAGACTACA TTCGTGTCCT CGACTTTGAT
    GTCAAGCGCA AATATTTCCG CCAAGAGCTG GAACGTCTGG ATGAGGGGCT CCGGAAAGAA
    GACATGGCTG TGCACGTCCG CCGTGATCAT GTGTTTGAAG ACTCCTATCG TGAACTGCAC
    CGGAAATCCC CTGAAGAAAT GAAGAATCGA TTGTATATAG TGTTTGAAGG AGAAGAAGGG
    CAGGATGCTG GAGGGCTCCT GCGGGAGTGG TATATGATCA TCTCTCGGGA GATGTTTAAC
    CCTATGTATG CCTTGTTCCG TACCTCACCC GGTGATCGGG TCACCTACAC CATCAACCCA
    TCTTCCCACT GCAACCCCAA TCACCTCAGC TACTTCAAGT TTGTTGGACG CATTGTGGCC
    AAAGCTGTAT ATGACAACCG TCTCCTGGAG TGCTATTTTA CTCGGTCCTT CTACAAACAC
    ATCTTGGGCA AGTCTGTCAG ATATACAGAT ATGGAGAGTG AAGATTACCA CTTCTACCAG
    GGCCTGGTTT ATCTGCTGGA AAATGATGTC TCCACACTAG GTTATGACCT CACCTTCAGC
    ACTGAGGTCC AGGAGTTTGG AGTCTGTGAA GTTCGTGACC TCAAACCCAA TGGGGCTAAC
    ATCTTGGTAA CTGAAGAGAA TAAGAAGGAA TATGTACACC TGGTGTGCCA GATGAGGATG
    ACAGGAGCCA TCCGTAAACA GCTGGCAGCT TTCTTAGAAG GCTTCTATGA GATCATTCCA
    AAGCGCCTCA TTTCTATCTT CACTGAGCAG GAGTTAGAGC TGCTCATATC AGGACTGCCC
    ACAATCGACA TTGATGATTT GAAATCCAAC ACTGAATACC ACAAGTACCA GTCCAACTCT
    ATTCAGATCC AGTGGTTCTG GAGAGCATTG CGTTCTTTCG ACCAAGCTGA CCGTGCCAAG
    TTCCTCCAAT TTGTCACGGG TACTTCAAAG GTGCCCTTAC AAGGCTTTGC TGCCCTTGAA
    GGCATGAATG GCATTCAGAA GTTTCAGATC CATCGGGATG ACAGGTCCAC AGATCGCCTG
    CCTTCAGCTC ACACATGTTT TAATCAGCTG GACCTACCTG CCTATGAGAG CTTTGAAAAG
    CTCCGCCACA TGTTACTATT GGCCATCCAG GAGTGCTCTG AAGGCTTTGG GCTGGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45535
    Clone ID Related Accession (Same CDS sequence) XM_014111528.2 , XM_014111528.1
    Accession Version XM_014111528.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006621.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
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    2941
    3001
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    3121
    3181
    3241
    3301
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    3421
    3481
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    4021
    4081
    4141
    4201
    4261
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    4381
    4441
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    4681
    4741
    4801
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    4921
    4981
    5041
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    5161
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    5281
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    5401
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    5521
    5581
    5641
    5701
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    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
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    7021
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    7141
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    9001
    9061
    9121
    9181
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    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG CAGACATCCC CCCATCCCCA
    GGAAATATCC CTACCACTCA CCCACTGATG GTACGCCATG CAGACCACAG TTCTCTGACA
    CTGGGCAGTG GCTCCTCCAC AACACGTCTC ACCCAGGGCA TCGGGCGCAG TCAGAGGACC
    CTAAGGCAGC TGACAGCCAA TACAGGCCAC ACCATTCATG TTCATTACCC TGGGAACCGC
    CAGCCCAACC CTCCTCTTAT ACTCCAGAGG TTGCTTGGTC CTTCTGCTGC TGCTGACATC
    CTTCAGCTGA GCAGCAGCCT TCCCCTACAA AGCCGTGGAC GAGCCCGCCT CCTGGTAGGC
    AATGATGATG TCCACATCAT TGCCAGATCT GATGATGAAC TGCTGGATGA CTTTTTCCAT
    GACCAGAGCA CAGCTACCAG CCAAGCAGGG ACCCTATCCA GCATCCCCAC AGCCCTGACC
    CGCTGGACAG AGGAATGCAA AGTTCTTGAT GCCGAGAGCA TGCATGACTG TGTTTCAGTT
    GTTAAAGTCC CCATTGTCAA TCACTTGGAA TTCCTGAGGG ATGAGGAGCT GGAAGAAAGG
    CGGGAGAAAC GCAGGAAACA ATTGGCTGAG GAAGAAACAA AGATAACTGA CAAAGGCAAA
    GAAGATAAGG AGAACAGGGA TCAGAGTGCA CAGTGTACTG CATCTAAAAC AAATGACTCC
    GCTGAACAGA ACCTCTCAGA TGGGACTCCT ATGCCTGACA GCTACCCAAC AACCCCATCT
    TCAACTGATG CAGCCACATC TGAGTCCAAG GATACCCTTG TCACTCTGCA GCCCTCACAA
    CAGCAACAAA CACTCCCACC CCCACCAGCC TTGGGAGAGA TTCCTCAAGA GCTACAGTCC
    CCGGCTGGAG AAGGGGGGAG CTCTACACAG CTGTTGATGC CTGTAGAGCC AGAGGAATTG
    GGTCCCACAA GGCCAAGTGG AGAAGCAGAA ACAACTCAGA TGGAGTTGTC CCCAGCTCCT
    ACTATAAGCA GAGATCTAAC CTCTCCATGT GACTTCCTCC TAGCCTCTCT TTCCCCAGAG
    AGGGCTGAAG ATTCTGATGC GCTGACAGCT GTCAGCAGTC AACTGGAAGG CTCTCCCATG
    GACACCAGTA GCCTGGCTTC CTGTACCCTA GAGGAGGCTG TGGGTGACAC CTCAGCAGCT
    GGCAGTTCTG AGCAGCCCAC AGCAGGCAGC TCCACTCCTG GGGATGCCCC AACTGTTGTG
    ACAGAAGTGC AAAGCAGGGG TGATGGGTCA GGTGAACCTA CCCAGCCCCC TGAAGACAGC
    TCTCCCCCTG CATCCTCTGA GAGTTCTTCC ACCAGAGATT CTGCTGTGGC CATTTCTGGA
    GCAGATTCCC GAGGAATCCT AGAAGAGCCA CTGCCTTCAA CAAGCAGTGA GGAAGAAGAT
    CCCCTTGCAG GTATCAGTCT ACCAGAAGGT GTGGACCCCT CTTTCCTGGC TGCCCTGCCT
    GATGACATCC GCCGGGAAGT CCTACAGAAC CAACTGGGCA TCCGTCCACC AACTCGGACT
    GCCCCTTCGG CAAATAGCTC AGCTCCTGCA GTGGTGGGGA ATCCTGGTGT GACCGAAGTG
    AGCCCTGAGT TCCTGGCGGC CCTGCCTCCA GCCATTCAGG AGGAAGTGCT GGCACAGCAG
    AGAGCTGAGC AACAACGACG GGAACTGGCA CAGAATGCGA GCTCAGACAC TCCCATGGAC
    CCTGTGACCT TCATCCAGAC CCTGCCATCA GACTTGCGCC GTAGTGTCCT GGAGGACATG
    GAGGACAGTG TTTTGGCTGT GATGCCACCT GACATTGCAG CTGAGGCTCA AGCCCTGAGG
    CGAGAGCAGG AAGCCCGGCA GCGACAGCTC ATGCATGAGC GGCTCTTTGG CCACAGCAGC
    ACCTCTGCGC TCTCTGCCAT CCTCCGAAGC CCGGCTTTCA CCAGTCGCCT CAGTGGCAAC
    CGAGGAGTCC AATACACTCG TCTTGCTGTG CAAAGAGGTG GCACCTTCCA GATGGGGGGT
    AGCAGCAGCC ATAACAGGCC TTCTGGCAGT AATGTGGATA CTCTTCTCCG CCTCCGAGGA
    CGGCTCCTCC TGGACCATGA AGCCCTATCC TGTCTCCTGG TCCTACTTTT CGTGGATGAG
    CCAAAGCTTA ATACTAGTCG TCTACACCGA GTACTGAGAA ATCTCTGCTA CCATGCCCAG
    ACCCGCCACT GGGTCATCCG CAGTCTGCTC TCCATTTTGC AGCGCAGCAG TGAAAGTGAG
    CTATGCATTG AAACACCCAA GCTCTCCTCA AGTGAGGACA AGGGCAAAAA GTCAAGCAAA
    AGCTGTGGGT CAAGCAGCCA TGAGAACCGG CCCCTGGACC TCCTACACAA GATGGAGTCT
    AAGAGCTCCA ACCAGCTTTC CTGGCTCTCA GTATCCATGG ATGCAGCCCT CGGCTGCAGG
    ACTAATATAT TCCAGATTCA ACGTTCAGGA GGTCGCAAAC ATACCGAGAA GCATGCAAGC
    AGTGGCTCCA CTGTCCACAT CCACCCCCAG GCTGCTCCTG TTGTCTGCAG ACATGTTCTA
    GACACACTCA TTCAATTGGC CAAGGTATTT CCCAGCCATT TTACACAGCA GCGGACCAAA
    GAAACAAACT GTGAGAGTGA TCGGGAGAGG GGCAGTAAGC AGGCCTGCAG CCCATGCTCT
    TCACAGTCCA CCAGCAGTGG GATTTGCACA GACTTCTGGG ACTTACTGGT TAAACTGGAC
    AACATGAATG TCAGCCGGAA AGGCAAGAAC TCCGTGAAGT CAGTGCCAGT GAGCGCTGGT
    GGTGAGGGGG AAACCTCCCC ATACAGCCTT GAGGCCTCTC CACTGGGGCA GCTCATGAAC
    ATGTTATCAC ACCCAGTCAT CCGCCGGAGC TCTCTCTTAA CTGAGAAACT CCTCAGACTC
    CTTTCTCTCA TCTCAATTGC TCTCCCAGAA AACAAAGTGT CGGAAGCACA GGCTAATTCT
    GGCAGCAGTG CTTCCTCCAC CACTGTTGCC ACCTCAACCA CATCTACCAC CACTACCACT
    GCTGCCTCCA GCACGCCCAC TCCCCCTGCT GCAACCACCC CTGTCACTTC TGCTCCAGCT
    CTGGTTGCTG CCACGGCTAT ATCCACCATT GCCGTAGCTG CTTCGACCAC GGTGACTACC
    CCCACGACTG CTACCACTAC TGTTTCAACT TCTACAACTA CTAAGGCCAG CAAATCTCCA
    GCAAAGGTGG GTGATGGGGG CAGCAGCAGT GCTGACTTTA AGATGGTATC CTCTGGCCTC
    ACTGAAAACC AGCTGCAGCT CTCTGTGGAG GTGTTGACAT CCCACTCTTG TTCCGAAGAA
    GGTCTAGAGG ATGCAGCCAA TGTGTTACTG CAGCTCTCTC GGGGGGACCC TGCAACCCGG
    GACACTGTTC TCAAGCTGCT ACTGAATGGA GCGCGCCATC TGGGTTATAC TCTTTGTAAA
    CAGATAGGTA CCCTTCTGGC TGAGTTACGG GAATACAACC TAGAACAGCA GCGACGAGCC
    CAATGTGAGA CCCTCTCTCC TGATGGCCTG CCTGAGGAGC AGCCACAGAC CACCAAGCTA
    AAAGGCAAAA TGCAGAGCAG GTTTGACATG GCTGAGAATG TGGTAATTGT GGCATCTCAG
    AAGCGACCTC TGGGTGGCCG GGAGCTCCAG CTGCCTTCTA TGTCCATGTT GACATCCAAG
    ACGTCTACCC AGAAGTTCTT CTTGAGGGTA CTGCAGGTCA TCATCCAGCT TCGGGACGAC
    ACACGCCGAG CTAACAAGAA AGCCAAGCAG ACAGGCAGGC TAGGTTCTTC CGGTTTAGGC
    TCAGCTAGCA GCATCCAGGC AGCTGTTCGG CAGCTGGAGG CTGAGGCTGA TGCCATTATA
    CAAATGGTAC GTGAGGGTCA AAGGGCGCGG AGACAGCAAC AAGCAGCAAC GTCGGAGTCA
    AGCCAGTCAG AGGCCTCTGT CCGGAGAGAG GAGTCACCCA TGGATGTGGA CCAGCCATCT
    CCCAGTGCTC AGGATACTCA GTCCATTGGC TCAGATGGAA CCCCACAAGG GGAAAAGGAG
    AAGGAGGAGA GGCCACCTGA GTTACCCCTG CTCAGTGAGC AGCTGAGCTT GGACGAGCTG
    TGGGACATGC TGGGAGAGTG TCTAAAGGAA CTGGAGGAAT CCCATGACCA GCATGCAGTG
    CTAGTGCTAC AGCCTGCCGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC
    AAACCTCCTG TCAGAGACAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA CGAGCCTCCT
    CCACTCTCCC CTGCCCCCTT AACCCCTGCT ACTCCTTCCT CCCTTGACCC ATTCTTCTCC
    AGGGAGCCCT CCTCTATGCA CATCTCCTCA AGCCTGCCCC CTGATACACA GAAGTTCCTT
    CGCTTTGCAG AGACTCACCG CACTGTGTTA AATCAGATCC TACGGCAGTC CACCACCCAC
    CTCGCTGATG GGCCTTTTGC TGTCCTGGTA GACTACATTC GTGTCCTCGA CTTTGATGTC
    AAGCGCAAAT ATTTCCGCCA AGAGCTGGAA CGTCTGGATG AGGGGCTCCG GAAAGAAGAC
    ATGGCTGTGC ACGTCCGCCG TGATCATGTG TTTGAAGACT CCTATCGTGA ACTGCACCGG
    AAATCCCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG
    GATGCTGGAG GGCTCCTGCG GGAGTGGTAT ATGATCATCT CTCGGGAGAT GTTTAACCCT
    ATGTATGCCT TGTTCCGTAC CTCACCCGGT GATCGGGTCA CCTACACCAT CAACCCATCT
    TCCCACTGCA ACCCCAATCA CCTCAGCTAC TTCAAGTTTG TTGGACGCAT TGTGGCCAAA
    GCTGTATATG ACAACCGTCT CCTGGAGTGC TATTTTACTC GGTCCTTCTA CAAACACATC
    TTGGGCAAGT CTGTCAGATA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC
    CTGGTTTATC TGCTGGAAAA TGATGTCTCC ACACTAGGTT ATGACCTCAC CTTCAGCACT
    GAGGTCCAGG AGTTTGGAGT CTGTGAAGTT CGTGACCTCA AACCCAATGG GGCTAACATC
    TTGGTAACTG AAGAGAATAA GAAGGAATAT GTACACCTGG TGTGCCAGAT GAGGATGACA
    GGAGCCATCC GTAAACAGCT GGCAGCTTTC TTAGAAGGCT TCTATGAGAT CATTCCAAAG
    CGCCTCATTT CTATCTTCAC TGAGCAGGAG TTAGAGCTGC TCATATCAGG ACTGCCCACA
    ATCGACATTG ATGATTTGAA ATCCAACACT GAATACCACA AGTACCAGTC CAACTCTATT
    CAGATCCAGT GGTTCTGGAG AGCATTGCGT TCTTTCGACC AAGCTGACCG TGCCAAGTTC
    CTCCAATTTG TCACGGGTAC TTCAAAGGTG CCCTTACAAG GCTTTGCTGC CCTTGAAGGC
    ATGAATGGCA TTCAGAAGTT TCAGATCCAT CGGGATGACA GGTCCACAGA TCGCCTGCCT
    TCAGCTCACA CATGTTTTAA TCAGCTGGAC CTACCTGCCT ATGAGAGCTT TGAAAAGCTC
    CGCCACATGT TACTATTGGC CATCCAGGAG TGCTCTGAAG GCTTTGGGCT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967003.1
    CDS361..13257
    Translation

    Target ORF information:

    RefSeq Version XM_014111528.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111528.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG CAGACATCCC CCCATCCCCA
    GGAAATATCC CTACCACTCA CCCACTGATG GTACGCCATG CAGACCACAG TTCTCTGACA
    CTGGGCAGTG GCTCCTCCAC AACACGTCTC ACCCAGGGCA TCGGGCGCAG TCAGAGGACC
    CTAAGGCAGC TGACAGCCAA TACAGGCCAC ACCATTCATG TTCATTACCC TGGGAACCGC
    CAGCCCAACC CTCCTCTTAT ACTCCAGAGG TTGCTTGGTC CTTCTGCTGC TGCTGACATC
    CTTCAGCTGA GCAGCAGCCT TCCCCTACAA AGCCGTGGAC GAGCCCGCCT CCTGGTAGGC
    AATGATGATG TCCACATCAT TGCCAGATCT GATGATGAAC TGCTGGATGA CTTTTTCCAT
    GACCAGAGCA CAGCTACCAG CCAAGCAGGG ACCCTATCCA GCATCCCCAC AGCCCTGACC
    CGCTGGACAG AGGAATGCAA AGTTCTTGAT GCCGAGAGCA TGCATGACTG TGTTTCAGTT
    GTTAAAGTCC CCATTGTCAA TCACTTGGAA TTCCTGAGGG ATGAGGAGCT GGAAGAAAGG
    CGGGAGAAAC GCAGGAAACA ATTGGCTGAG GAAGAAACAA AGATAACTGA CAAAGGCAAA
    GAAGATAAGG AGAACAGGGA TCAGAGTGCA CAGTGTACTG CATCTAAAAC AAATGACTCC
    GCTGAACAGA ACCTCTCAGA TGGGACTCCT ATGCCTGACA GCTACCCAAC AACCCCATCT
    TCAACTGATG CAGCCACATC TGAGTCCAAG GATACCCTTG TCACTCTGCA GCCCTCACAA
    CAGCAACAAA CACTCCCACC CCCACCAGCC TTGGGAGAGA TTCCTCAAGA GCTACAGTCC
    CCGGCTGGAG AAGGGGGGAG CTCTACACAG CTGTTGATGC CTGTAGAGCC AGAGGAATTG
    GGTCCCACAA GGCCAAGTGG AGAAGCAGAA ACAACTCAGA TGGAGTTGTC CCCAGCTCCT
    ACTATAAGCA GAGATCTAAC CTCTCCATGT GACTTCCTCC TAGCCTCTCT TTCCCCAGAG
    AGGGCTGAAG ATTCTGATGC GCTGACAGCT GTCAGCAGTC AACTGGAAGG CTCTCCCATG
    GACACCAGTA GCCTGGCTTC CTGTACCCTA GAGGAGGCTG TGGGTGACAC CTCAGCAGCT
    GGCAGTTCTG AGCAGCCCAC AGCAGGCAGC TCCACTCCTG GGGATGCCCC AACTGTTGTG
    ACAGAAGTGC AAAGCAGGGG TGATGGGTCA GGTGAACCTA CCCAGCCCCC TGAAGACAGC
    TCTCCCCCTG CATCCTCTGA GAGTTCTTCC ACCAGAGATT CTGCTGTGGC CATTTCTGGA
    GCAGATTCCC GAGGAATCCT AGAAGAGCCA CTGCCTTCAA CAAGCAGTGA GGAAGAAGAT
    CCCCTTGCAG GTATCAGTCT ACCAGAAGGT GTGGACCCCT CTTTCCTGGC TGCCCTGCCT
    GATGACATCC GCCGGGAAGT CCTACAGAAC CAACTGGGCA TCCGTCCACC AACTCGGACT
    GCCCCTTCGG CAAATAGCTC AGCTCCTGCA GTGGTGGGGA ATCCTGGTGT GACCGAAGTG
    AGCCCTGAGT TCCTGGCGGC CCTGCCTCCA GCCATTCAGG AGGAAGTGCT GGCACAGCAG
    AGAGCTGAGC AACAACGACG GGAACTGGCA CAGAATGCGA GCTCAGACAC TCCCATGGAC
    CCTGTGACCT TCATCCAGAC CCTGCCATCA GACTTGCGCC GTAGTGTCCT GGAGGACATG
    GAGGACAGTG TTTTGGCTGT GATGCCACCT GACATTGCAG CTGAGGCTCA AGCCCTGAGG
    CGAGAGCAGG AAGCCCGGCA GCGACAGCTC ATGCATGAGC GGCTCTTTGG CCACAGCAGC
    ACCTCTGCGC TCTCTGCCAT CCTCCGAAGC CCGGCTTTCA CCAGTCGCCT CAGTGGCAAC
    CGAGGAGTCC AATACACTCG TCTTGCTGTG CAAAGAGGTG GCACCTTCCA GATGGGGGGT
    AGCAGCAGCC ATAACAGGCC TTCTGGCAGT AATGTGGATA CTCTTCTCCG CCTCCGAGGA
    CGGCTCCTCC TGGACCATGA AGCCCTATCC TGTCTCCTGG TCCTACTTTT CGTGGATGAG
    CCAAAGCTTA ATACTAGTCG TCTACACCGA GTACTGAGAA ATCTCTGCTA CCATGCCCAG
    ACCCGCCACT GGGTCATCCG CAGTCTGCTC TCCATTTTGC AGCGCAGCAG TGAAAGTGAG
    CTATGCATTG AAACACCCAA GCTCTCCTCA AGTGAGGACA AGGGCAAAAA GTCAAGCAAA
    AGCTGTGGGT CAAGCAGCCA TGAGAACCGG CCCCTGGACC TCCTACACAA GATGGAGTCT
    AAGAGCTCCA ACCAGCTTTC CTGGCTCTCA GTATCCATGG ATGCAGCCCT CGGCTGCAGG
    ACTAATATAT TCCAGATTCA ACGTTCAGGA GGTCGCAAAC ATACCGAGAA GCATGCAAGC
    AGTGGCTCCA CTGTCCACAT CCACCCCCAG GCTGCTCCTG TTGTCTGCAG ACATGTTCTA
    GACACACTCA TTCAATTGGC CAAGGTATTT CCCAGCCATT TTACACAGCA GCGGACCAAA
    GAAACAAACT GTGAGAGTGA TCGGGAGAGG GGCAGTAAGC AGGCCTGCAG CCCATGCTCT
    TCACAGTCCA CCAGCAGTGG GATTTGCACA GACTTCTGGG ACTTACTGGT TAAACTGGAC
    AACATGAATG TCAGCCGGAA AGGCAAGAAC TCCGTGAAGT CAGTGCCAGT GAGCGCTGGT
    GGTGAGGGGG AAACCTCCCC ATACAGCCTT GAGGCCTCTC CACTGGGGCA GCTCATGAAC
    ATGTTATCAC ACCCAGTCAT CCGCCGGAGC TCTCTCTTAA CTGAGAAACT CCTCAGACTC
    CTTTCTCTCA TCTCAATTGC TCTCCCAGAA AACAAAGTGT CGGAAGCACA GGCTAATTCT
    GGCAGCAGTG CTTCCTCCAC CACTGTTGCC ACCTCAACCA CATCTACCAC CACTACCACT
    GCTGCCTCCA GCACGCCCAC TCCCCCTGCT GCAACCACCC CTGTCACTTC TGCTCCAGCT
    CTGGTTGCTG CCACGGCTAT ATCCACCATT GCCGTAGCTG CTTCGACCAC GGTGACTACC
    CCCACGACTG CTACCACTAC TGTTTCAACT TCTACAACTA CTAAGGCCAG CAAATCTCCA
    GCAAAGGTGG GTGATGGGGG CAGCAGCAGT GCTGACTTTA AGATGGTATC CTCTGGCCTC
    ACTGAAAACC AGCTGCAGCT CTCTGTGGAG GTGTTGACAT CCCACTCTTG TTCCGAAGAA
    GGTCTAGAGG ATGCAGCCAA TGTGTTACTG CAGCTCTCTC GGGGGGACCC TGCAACCCGG
    GACACTGTTC TCAAGCTGCT ACTGAATGGA GCGCGCCATC TGGGTTATAC TCTTTGTAAA
    CAGATAGGTA CCCTTCTGGC TGAGTTACGG GAATACAACC TAGAACAGCA GCGACGAGCC
    CAATGTGAGA CCCTCTCTCC TGATGGCCTG CCTGAGGAGC AGCCACAGAC CACCAAGCTA
    AAAGGCAAAA TGCAGAGCAG GTTTGACATG GCTGAGAATG TGGTAATTGT GGCATCTCAG
    AAGCGACCTC TGGGTGGCCG GGAGCTCCAG CTGCCTTCTA TGTCCATGTT GACATCCAAG
    ACGTCTACCC AGAAGTTCTT CTTGAGGGTA CTGCAGGTCA TCATCCAGCT TCGGGACGAC
    ACACGCCGAG CTAACAAGAA AGCCAAGCAG ACAGGCAGGC TAGGTTCTTC CGGTTTAGGC
    TCAGCTAGCA GCATCCAGGC AGCTGTTCGG CAGCTGGAGG CTGAGGCTGA TGCCATTATA
    CAAATGGTAC GTGAGGGTCA AAGGGCGCGG AGACAGCAAC AAGCAGCAAC GTCGGAGTCA
    AGCCAGTCAG AGGCCTCTGT CCGGAGAGAG GAGTCACCCA TGGATGTGGA CCAGCCATCT
    CCCAGTGCTC AGGATACTCA GTCCATTGGC TCAGATGGAA CCCCACAAGG GGAAAAGGAG
    AAGGAGGAGA GGCCACCTGA GTTACCCCTG CTCAGTGAGC AGCTGAGCTT GGACGAGCTG
    TGGGACATGC TGGGAGAGTG TCTAAAGGAA CTGGAGGAAT CCCATGACCA GCATGCAGTG
    CTAGTGCTAC AGCCTGCCGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC
    AAACCTCCTG TCAGAGACAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA CGAGCCTCCT
    CCACTCTCCC CTGCCCCCTT AACCCCTGCT ACTCCTTCCT CCCTTGACCC ATTCTTCTCC
    AGGGAGCCCT CCTCTATGCA CATCTCCTCA AGCCTGCCCC CTGATACACA GAAGTTCCTT
    CGCTTTGCAG AGACTCACCG CACTGTGTTA AATCAGATCC TACGGCAGTC CACCACCCAC
    CTCGCTGATG GGCCTTTTGC TGTCCTGGTA GACTACATTC GTGTCCTCGA CTTTGATGTC
    AAGCGCAAAT ATTTCCGCCA AGAGCTGGAA CGTCTGGATG AGGGGCTCCG GAAAGAAGAC
    ATGGCTGTGC ACGTCCGCCG TGATCATGTG TTTGAAGACT CCTATCGTGA ACTGCACCGG
    AAATCCCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG
    GATGCTGGAG GGCTCCTGCG GGAGTGGTAT ATGATCATCT CTCGGGAGAT GTTTAACCCT
    ATGTATGCCT TGTTCCGTAC CTCACCCGGT GATCGGGTCA CCTACACCAT CAACCCATCT
    TCCCACTGCA ACCCCAATCA CCTCAGCTAC TTCAAGTTTG TTGGACGCAT TGTGGCCAAA
    GCTGTATATG ACAACCGTCT CCTGGAGTGC TATTTTACTC GGTCCTTCTA CAAACACATC
    TTGGGCAAGT CTGTCAGATA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC
    CTGGTTTATC TGCTGGAAAA TGATGTCTCC ACACTAGGTT ATGACCTCAC CTTCAGCACT
    GAGGTCCAGG AGTTTGGAGT CTGTGAAGTT CGTGACCTCA AACCCAATGG GGCTAACATC
    TTGGTAACTG AAGAGAATAA GAAGGAATAT GTACACCTGG TGTGCCAGAT GAGGATGACA
    GGAGCCATCC GTAAACAGCT GGCAGCTTTC TTAGAAGGCT TCTATGAGAT CATTCCAAAG
    CGCCTCATTT CTATCTTCAC TGAGCAGGAG TTAGAGCTGC TCATATCAGG ACTGCCCACA
    ATCGACATTG ATGATTTGAA ATCCAACACT GAATACCACA AGTACCAGTC CAACTCTATT
    CAGATCCAGT GGTTCTGGAG AGCATTGCGT TCTTTCGACC AAGCTGACCG TGCCAAGTTC
    CTCCAATTTG TCACGGGTAC TTCAAAGGTG CCCTTACAAG GCTTTGCTGC CCTTGAAGGC
    ATGAATGGCA TTCAGAAGTT TCAGATCCAT CGGGATGACA GGTCCACAGA TCGCCTGCCT
    TCAGCTCACA CATGTTTTAA TCAGCTGGAC CTACCTGCCT ATGAGAGCTT TGAAAAGCTC
    CGCCACATGT TACTATTGGC CATCCAGGAG TGCTCTGAAG GCTTTGGGCT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45535
    Clone ID Related Accession (Same CDS sequence) XM_014111528.2 , XM_014111528.1
    Accession Version XM_014111528.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 12897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase HUWE1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726126.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
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    2641
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    11521
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    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG CAGACATCCC CCCATCCCCA
    GGAAATATCC CTACCACTCA CCCACTGATG GTACGCCATG CAGACCACAG TTCTCTGACA
    CTGGGCAGTG GCTCCTCCAC AACACGTCTC ACCCAGGGCA TCGGGCGCAG TCAGAGGACC
    CTAAGGCAGC TGACAGCCAA TACAGGCCAC ACCATTCATG TTCATTACCC TGGGAACCGC
    CAGCCCAACC CTCCTCTTAT ACTCCAGAGG TTGCTTGGTC CTTCTGCTGC TGCTGACATC
    CTTCAGCTGA GCAGCAGCCT TCCCCTACAA AGCCGTGGAC GAGCCCGCCT CCTGGTAGGC
    AATGATGATG TCCACATCAT TGCCAGATCT GATGATGAAC TGCTGGATGA CTTTTTCCAT
    GACCAGAGCA CAGCTACCAG CCAAGCAGGG ACCCTATCCA GCATCCCCAC AGCCCTGACC
    CGCTGGACAG AGGAATGCAA AGTTCTTGAT GCCGAGAGCA TGCATGACTG TGTTTCAGTT
    GTTAAAGTCC CCATTGTCAA TCACTTGGAA TTCCTGAGGG ATGAGGAGCT GGAAGAAAGG
    CGGGAGAAAC GCAGGAAACA ATTGGCTGAG GAAGAAACAA AGATAACTGA CAAAGGCAAA
    GAAGATAAGG AGAACAGGGA TCAGAGTGCA CAGTGTACTG CATCTAAAAC AAATGACTCC
    GCTGAACAGA ACCTCTCAGA TGGGACTCCT ATGCCTGACA GCTACCCAAC AACCCCATCT
    TCAACTGATG CAGCCACATC TGAGTCCAAG GATACCCTTG TCACTCTGCA GCCCTCACAA
    CAGCAACAAA CACTCCCACC CCCACCAGCC TTGGGAGAGA TTCCTCAAGA GCTACAGTCC
    CCGGCTGGAG AAGGGGGGAG CTCTACACAG CTGTTGATGC CTGTAGAGCC AGAGGAATTG
    GGTCCCACAA GGCCAAGTGG AGAAGCAGAA ACAACTCAGA TGGAGTTGTC CCCAGCTCCT
    ACTATAAGCA GAGATCTAAC CTCTCCATGT GACTTCCTCC TAGCCTCTCT TTCCCCAGAG
    AGGGCTGAAG ATTCTGATGC GCTGACAGCT GTCAGCAGTC AACTGGAAGG CTCTCCCATG
    GACACCAGTA GCCTGGCTTC CTGTACCCTA GAGGAGGCTG TGGGTGACAC CTCAGCAGCT
    GGCAGTTCTG AGCAGCCCAC AGCAGGCAGC TCCACTCCTG GGGATGCCCC AACTGTTGTG
    ACAGAAGTGC AAAGCAGGGG TGATGGGTCA GGTGAACCTA CCCAGCCCCC TGAAGACAGC
    TCTCCCCCTG CATCCTCTGA GAGTTCTTCC ACCAGAGATT CTGCTGTGGC CATTTCTGGA
    GCAGATTCCC GAGGAATCCT AGAAGAGCCA CTGCCTTCAA CAAGCAGTGA GGAAGAAGAT
    CCCCTTGCAG GTATCAGTCT ACCAGAAGGT GTGGACCCCT CTTTCCTGGC TGCCCTGCCT
    GATGACATCC GCCGGGAAGT CCTACAGAAC CAACTGGGCA TCCGTCCACC AACTCGGACT
    GCCCCTTCGG CAAATAGCTC AGCTCCTGCA GTGGTGGGGA ATCCTGGTGT GACCGAAGTG
    AGCCCTGAGT TCCTGGCGGC CCTGCCTCCA GCCATTCAGG AGGAAGTGCT GGCACAGCAG
    AGAGCTGAGC AACAACGACG GGAACTGGCA CAGAATGCGA GCTCAGACAC TCCCATGGAC
    CCTGTGACCT TCATCCAGAC CCTGCCATCA GACTTGCGCC GTAGTGTCCT GGAGGACATG
    GAGGACAGTG TTTTGGCTGT GATGCCACCT GACATTGCAG CTGAGGCTCA AGCCCTGAGG
    CGAGAGCAGG AAGCCCGGCA GCGACAGCTC ATGCATGAGC GGCTCTTTGG CCACAGCAGC
    ACCTCTGCGC TCTCTGCCAT CCTCCGAAGC CCGGCTTTCA CCAGTCGCCT CAGTGGCAAC
    CGAGGAGTCC AATACACTCG TCTTGCTGTG CAAAGAGGTG GCACCTTCCA GATGGGGGGT
    AGCAGCAGCC ATAACAGGCC TTCTGGCAGT AATGTGGATA CTCTTCTCCG CCTCCGAGGA
    CGGCTCCTCC TGGACCATGA AGCCCTATCC TGTCTCCTGG TCCTACTTTT CGTGGATGAG
    CCAAAGCTTA ATACTAGTCG TCTACACCGA GTACTGAGAA ATCTCTGCTA CCATGCCCAG
    ACCCGCCACT GGGTCATCCG CAGTCTGCTC TCCATTTTGC AGCGCAGCAG TGAAAGTGAG
    CTATGCATTG AAACACCCAA GCTCTCCTCA AGTGAGGACA AGGGCAAAAA GTCAAGCAAA
    AGCTGTGGGT CAAGCAGCCA TGAGAACCGG CCCCTGGACC TCCTACACAA GATGGAGTCT
    AAGAGCTCCA ACCAGCTTTC CTGGCTCTCA GTATCCATGG ATGCAGCCCT CGGCTGCAGG
    ACTAATATAT TCCAGATTCA ACGTTCAGGA GGTCGCAAAC ATACCGAGAA GCATGCAAGC
    AGTGGCTCCA CTGTCCACAT CCACCCCCAG GCTGCTCCTG TTGTCTGCAG ACATGTTCTA
    GACACACTCA TTCAATTGGC CAAGGTATTT CCCAGCCATT TTACACAGCA GCGGACCAAA
    GAAACAAACT GTGAGAGTGA TCGGGAGAGG GGCAGTAAGC AGGCCTGCAG CCCATGCTCT
    TCACAGTCCA CCAGCAGTGG GATTTGCACA GACTTCTGGG ACTTACTGGT TAAACTGGAC
    AACATGAATG TCAGCCGGAA AGGCAAGAAC TCCGTGAAGT CAGTGCCAGT GAGCGCTGGT
    GGTGAGGGGG AAACCTCCCC ATACAGCCTT GAGGCCTCTC CACTGGGGCA GCTCATGAAC
    ATGTTATCAC ACCCAGTCAT CCGCCGGAGC TCTCTCTTAA CTGAGAAACT CCTCAGACTC
    CTTTCTCTCA TCTCAATTGC TCTCCCAGAA AACAAAGTGT CGGAAGCACA GGCTAATTCT
    GGCAGCAGTG CTTCCTCCAC CACTGTTGCC ACCTCAACCA CATCTACCAC CACTACCACT
    GCTGCCTCCA GCACGCCCAC TCCCCCTGCT GCAACCACCC CTGTCACTTC TGCTCCAGCT
    CTGGTTGCTG CCACGGCTAT ATCCACCATT GCCGTAGCTG CTTCGACCAC GGTGACTACC
    CCCACGACTG CTACCACTAC TGTTTCAACT TCTACAACTA CTAAGGCCAG CAAATCTCCA
    GCAAAGGTGG GTGATGGGGG CAGCAGCAGT GCTGACTTTA AGATGGTATC CTCTGGCCTC
    ACTGAAAACC AGCTGCAGCT CTCTGTGGAG GTGTTGACAT CCCACTCTTG TTCCGAAGAA
    GGTCTAGAGG ATGCAGCCAA TGTGTTACTG CAGCTCTCTC GGGGGGACCC TGCAACCCGG
    GACACTGTTC TCAAGCTGCT ACTGAATGGA GCGCGCCATC TGGGTTATAC TCTTTGTAAA
    CAGATAGGTA CCCTTCTGGC TGAGTTACGG GAATACAACC TAGAACAGCA GCGACGAGCC
    CAATGTGAGA CCCTCTCTCC TGATGGCCTG CCTGAGGAGC AGCCACAGAC CACCAAGCTA
    AAAGGCAAAA TGCAGAGCAG GTTTGACATG GCTGAGAATG TGGTAATTGT GGCATCTCAG
    AAGCGACCTC TGGGTGGCCG GGAGCTCCAG CTGCCTTCTA TGTCCATGTT GACATCCAAG
    ACGTCTACCC AGAAGTTCTT CTTGAGGGTA CTGCAGGTCA TCATCCAGCT TCGGGACGAC
    ACACGCCGAG CTAACAAGAA AGCCAAGCAG ACAGGCAGGC TAGGTTCTTC CGGTTTAGGC
    TCAGCTAGCA GCATCCAGGC AGCTGTTCGG CAGCTGGAGG CTGAGGCTGA TGCCATTATA
    CAAATGGTAC GTGAGGGTCA AAGGGCGCGG AGACAGCAAC AAGCAGCAAC GTCGGAGTCA
    AGCCAGTCAG AGGCCTCTGT CCGGAGAGAG GAGTCACCCA TGGATGTGGA CCAGCCATCT
    CCCAGTGCTC AGGATACTCA GTCCATTGGC TCAGATGGAA CCCCACAAGG GGAAAAGGAG
    AAGGAGGAGA GGCCACCTGA GTTACCCCTG CTCAGTGAGC AGCTGAGCTT GGACGAGCTG
    TGGGACATGC TGGGAGAGTG TCTAAAGGAA CTGGAGGAAT CCCATGACCA GCATGCAGTG
    CTAGTGCTAC AGCCTGCCGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC
    AAACCTCCTG TCAGAGACAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA CGAGCCTCCT
    CCACTCTCCC CTGCCCCCTT AACCCCTGCT ACTCCTTCCT CCCTTGACCC ATTCTTCTCC
    AGGGAGCCCT CCTCTATGCA CATCTCCTCA AGCCTGCCCC CTGATACACA GAAGTTCCTT
    CGCTTTGCAG AGACTCACCG CACTGTGTTA AATCAGATCC TACGGCAGTC CACCACCCAC
    CTCGCTGATG GGCCTTTTGC TGTCCTGGTA GACTACATTC GTGTCCTCGA CTTTGATGTC
    AAGCGCAAAT ATTTCCGCCA AGAGCTGGAA CGTCTGGATG AGGGGCTCCG GAAAGAAGAC
    ATGGCTGTGC ACGTCCGCCG TGATCATGTG TTTGAAGACT CCTATCGTGA ACTGCACCGG
    AAATCCCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG
    GATGCTGGAG GGCTCCTGCG GGAGTGGTAT ATGATCATCT CTCGGGAGAT GTTTAACCCT
    ATGTATGCCT TGTTCCGTAC CTCACCCGGT GATCGGGTCA CCTACACCAT CAACCCATCT
    TCCCACTGCA ACCCCAATCA CCTCAGCTAC TTCAAGTTTG TTGGACGCAT TGTGGCCAAA
    GCTGTATATG ACAACCGTCT CCTGGAGTGC TATTTTACTC GGTCCTTCTA CAAACACATC
    TTGGGCAAGT CTGTCAGATA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC
    CTGGTTTATC TGCTGGAAAA TGATGTCTCC ACACTAGGTT ATGACCTCAC CTTCAGCACT
    GAGGTCCAGG AGTTTGGAGT CTGTGAAGTT CGTGACCTCA AACCCAATGG GGCTAACATC
    TTGGTAACTG AAGAGAATAA GAAGGAATAT GTACACCTGG TGTGCCAGAT GAGGATGACA
    GGAGCCATCC GTAAACAGCT GGCAGCTTTC TTAGAAGGCT TCTATGAGAT CATTCCAAAG
    CGCCTCATTT CTATCTTCAC TGAGCAGGAG TTAGAGCTGC TCATATCAGG ACTGCCCACA
    ATCGACATTG ATGATTTGAA ATCCAACACT GAATACCACA AGTACCAGTC CAACTCTATT
    CAGATCCAGT GGTTCTGGAG AGCATTGCGT TCTTTCGACC AAGCTGACCG TGCCAAGTTC
    CTCCAATTTG TCACGGGTAC TTCAAAGGTG CCCTTACAAG GCTTTGCTGC CCTTGAAGGC
    ATGAATGGCA TTCAGAAGTT TCAGATCCAT CGGGATGACA GGTCCACAGA TCGCCTGCCT
    TCAGCTCACA CATGTTTTAA TCAGCTGGAC CTACCTGCCT ATGAGAGCTT TGAAAAGCTC
    CGCCACATGT TACTATTGGC CATCCAGGAG TGCTCTGAAG GCTTTGGGCT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013967003.1
    CDS452..13348
    Translation

    Target ORF information:

    RefSeq Version XM_014111528.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase (HUWE1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111528.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    ATGAAAGTAG ACAGGACTAA ACTGAAAAAG ACACCTACTG AGGCTCCTGC AGACTGCAGA 
    GCCTTAATAG ACAAACTCAA AGTTTGTAAT GATGAGCAAC TCCTCCTGGA ACTGCAACAG
    ATCAAAACAT GGAACATTGG GAAGTGTGAG TTATACCACT GGGTGGATCT CTTGGACCGC
    TTTGATGGAA TCCTGGCAGA TGCTGGACAG ACAGTGGAGA ATATGTCATG GATGCTTGTA
    TGTGATAGGC CGGAAAGAGA ACAACTGAAA ATGCTTCTCT TGGCTGTGTT GAACTTCACA
    GCCTTGCTCA TTGAGTATAG CTTTTCCCGA CATCTGTACA GTTCCATAGA GCATTTGACA
    ACTTTATTGG CTTCCTCTGA TATGCAAGTG GTGCTGGCAG TCCTTAATCT TCTGTATGTA
    TTTAGCAAAA GATCAAACTA TATCACACGC CTTGGATCTG ACAAGAGAAC CCCACTGCTA
    ACCCGGCTGC AACACTTGGC AGAGAGCTGG GGTGGAAAGG AAAATGGCTT TGGACTTGCA
    GAATGTTGCA GAGACTTGCA TATGATGAAA TACCCACCCA GTGCAACTAC ACTACACTTT
    GAATTCTATG CAGACCCTGG GGCCGAGGTC AAAATCGAGA AAAGGACAAC TAGTAACACA
    CTACATTATA TTCACATAGA GCAACTTGAC AAGATTTCAG AAAGCCCTTC TGAAATCATG
    GAATCTCTTA CCAAAATGTA CAGCATTCCT AAGGATAAGC AGATGCTGTT ATTTACGCAC
    ATACGGCTGG CCCATGGCTT TTCTAATCAC AGGAAACGAT TGCAAGCTGT TCAAGCCAGG
    CTGCATGCAA TATCTATATT AGTGTATTCC AATGCCTTGC AGGAGTCAGC AAACAGTATC
    TTGTATAATG GGTTGATAGA GGAGTTGGTA GATGTCCTTC AGATAACAGA TAAGCAGCTT
    ATGGAGATTA AAGCCGCTTC TTTACGAACA TTAACATCAA TTGTCCACTT GGAGAGAACT
    CCCAAACTCA GCAGTATTAT TGACTGTACT GGAACTGCCT CTTACCATGG ATTTTTGCCT
    GTACTTGTGC GGAACTGTAT CCAGGCCATG ATTGATCCTT CCATGGATCC ATATCCTCAC
    CAGTTTGCCA CTGCTCTCTT CTCTTTTTTA TACCATCTGG CCAGCTACGA TGCTGGTGGT
    GAAGCCTTGG TCTCCTGTGG AATGATGGAA GCCTTATTGA AGGTCATAAA GTTTCTTGGC
    GATGAACAGG ACCAGATAAC ATTTGTCACC AGAGCCGTCA GAGTGGTTGA CCTCATCACC
    AACCTGGACA TGGCAGCTTT TCAATCCCAT AGTGGACTTT CTATCTTCAT TTATAGACTT
    GAGCATGAAG TTGATTTGTG TCGAAAAGAA TGTCCATTTG TGATCAAGCC AAAGATCCAG
    AGACCCAGTA CTACACAAGA AGGAGAAGAA ATGGAAACTG ATATGGATGT TGCAGATGTG
    ACTATGGAAA GTAGTCCAGG CTCATCTGTC TCTGTGGAAC ACCGGCTAGA TGTTGAATTA
    AGGGCATCAA GTTCTAGCAG CAGCGCTAGC ATCTCTTCTG GCTCTGGTCC TGGCCCCCGT
    CCTGGAGTCC AGTGTATTCC ACAAAGAGCA GCACTTCTCA AATCCATGTT GAATTTCCTC
    AAGAAGGCCA TTCAAGACCC TGCTTTCTCA GATGGCATAC GACATGTGAT GGACGGTTCT
    CTCCCTACCT CCCTGAAGCA CATCATCAGT AATGCAGAAT ATTATGGCCC ATCACTCTTC
    CTTCTAGCTA CTGAAGTGGT GACTGTGTTT GTATTTCAAG AACCATCACT GCTCTCCTCA
    CTCCAGGACA ATGGATTAAC AGATGTCATG CTTCATGCAC TGCTTATCAA AGATGTTCCT
    GCTACCCGTG AAGTCCTTGG CTCCCTCCCA AATGTATTCA GTGCACTCTG TTTGAATGCC
    CGAGGTCTTC AATCATTTGT ACAGTGTCAG CCTTTTGAAC GTCTTTTCAA AGTTCTTCTG
    TCTCCAGATT ATCTCCCAGC CATGCGAAGG AGGAGGAGTT CTGATCCTCT TGGGGATACT
    GCATCTAACC TGGGGAGTGC TGTTGATGAG CTCATGAGAC ATCAGCCCAC CCTCAAAACA
    GATGCAACGA CTGCGATCAT CAAGTTACTT GAAGAAATCT GTAATCTTGG AAGAGACCCT
    AAATACATCT GTCAGAAGCC ATCAATCCAG AAGGCAGATG GCACTGCCAC TGCTCCTCCT
    CCAAGGTCTA ATCATGCTGC AGAAGAGGCC TCTAGTGAGG ATGAAGAGGA GGAAGAAGTA
    CAGGCCATGC AGAGCTTTAA TTCTACCCAA CAGAATGAAA CAGAGCCCAA TCAGCAGGTT
    GTTGGTACAG AGGAACGTAT TCCTATTCCC CTCATGGATT ACATCCTTAA TGTGATGAAA
    TTTGTGGAAT CTATTCTGAG CAACAATACA ACAGATGACC ACTGCCAGGA ATTTGTGAAT
    CAGAAAGGAC TCTTGCCTTT GGTTACCATT TTGGGTCTTC CTAATCTGCC CATTGACTTT
    CCCACATCTG CGGCCTGTCA GGCTGTTGCA GGTGTCTGCA AATCCATATT GACACTGTCA
    CACGAACCCA AAGTTCTTCA AGAGGGTCTT CTTCAGTTGG ACTCCATCCT TTCATCCTTG
    GAGCCCTTAC ATCGCCCAAT TGAATCCCCT GGAGGCTCAG TATTATTGCG AGAACTGGCT
    TGTGCTGGCA ATGTTGCTGA TGCTACCCTC TCAGCTCAGG CCACACCTCT ACTGCATGCA
    CTCACTGCTG CCCATGCCTA CATCATGATG TTTGTTCATA CTTGCAGAGT TGGACAGAGT
    GAAATTCGTT CCATCTCTGT AAACCAGTGG GGCTCTCAGT TGGGTCTGAG TGTTTTGAGC
    AAGCTGAGCC AGTTATACTG TTCCCTGGTG TGGGAAAGCA CTGTCCTTCT CTCCCTGTGT
    ACCCCAAACA GTCTGCCATC TGGGTGTGAA TTTGGCCAGG TGGACATGCA GAAACTTGTT
    CCAAAGGATG AGAAGGCAGG TACGACCCAG GGCGGAAAAA GATCAGATGG GGAACAGGAT
    GGAACAGCTG CAAGTATGGA TGCTTCTACC CAGGGCTTAT TAGAAGGCAT TGGGCTTGAT
    GGTGACACAT TGGCTCCCAT GGAAACAGAT GAACCTACTG CTTCAGACTC GAAAGGCAAA
    TCTAAAATTA CACCTGCTAT GGCTGCCAGA ATCAAGCAAA TCAAGCCTTT ATTGTCAGCT
    TCCTCCAGAT TAGGTCGAGC ACTTGCTGAA CTATTTGGAC TCCTTGTTAA ATTATGTGTG
    GGGTCTCCTG TCCGCCAAAG AAGGAGCCAT CATGCTGCCA GCACTACTAC AGCACCAACA
    CCTGCTGCTC GATCCACAGC CTCAGCCCTC ACTAAGCTCC TCACAAAGGG CTTATCTTGG
    CAACCTCCAC CATATACACC AACTCCCCGC TTCAGGCTGA CATTCTTCAT CTGTTCAGTT
    GGTTTTACAT CCCCAATGCT GTTTGATGAG AGGAAGTATC CTTACCACCT CATGCTGCAG
    AAATTTCTCT GCTCTGGAGG CCACAATGCT CTTTTTGAAA CTTTCAATTG GGCTCTGTCC
    ATGGGAGGCA AAGTTCCTGT TGCTGAAGGA TTAGAACATT CAGATTTACC TGATGGCACA
    GGAGAATTCC TAGATGCCTG GCTAATGCTA GTAGAGAAGA TGGTGAATCC CACCACGGTG
    CTTGAATCTC CACATTCACT GCCTGCCAAA TTGCCTGGAG GTGTCCAGAA CTTTCCACAG
    TTCAGTGCCC TTCGCTTCCT TGTGGTAACT CAGAAAGCAG CCTTTACTTG CATCAAGAAC
    CTGTGGAACC GGAAACCCCT GAAAGTATAT GGCGGGCGAA TGGCTGAGTC CATGCTGGCT
    ATCCTATGCC ACATTCTCCG AGGAGAACCT GTGATTCGAG AGAGACTGAG CAAAGAGAAA
    GAGGGATCTC GAGGAGAAGA GGACACAGGG CAAGAGGAAG GTGGCTCTCG CCGGGAACCT
    CAAGTCAACC AGCAGCAACT ACAACAGCTT ATGGACATGG GCTTCACAAG GGAACATGCC
    ATGGAGGCCC TGTTGAACAC CAGCACCATG GAGCAGGCCA CAGAGTACCT TCTAACTCAC
    CCTCCCCCAA TTATGGGAGG AGTTGTTCGG GATCTGAGTA TGTCCGAAGA GGACCAGATG
    ATGAGAGCAA TTGCTATGTC TCTGGGACAG GATATCCCAA TGGATCAAAG AGCGGAGTCA
    CCTGAGGAAG TTGCTTGTCG GAAAGAAGAG GAGGAGCGGA AAGCTCGGGA AAAGCAGGAA
    GAGGAAGAAG CTAAATGTCT AGAGAAGTTC CAGGATGCTG ATCCATTGGA ACAAGATGAG
    CTCCACACTT TCACAGATAC CATGTTACCA GGCTGCTTTC ACCTTCTTGA TGAGCTGCCG
    GACACAGTGT ACCGGGTGTG TGATTTAATC ATGACCGCAA TCAAACGTAA TGGAGCTGAC
    TATCGAGACA TGATTCTAAA ACAAGTAGTC AATCAGGTAT GGGAAGCTGC TGATGTATTG
    ATCAAAGCTG CTCTTCCCCT GACAACAAGT GACACAAAAA CTGTGTCAGA GTGGATCAGT
    CAGATGGCCA CCCTGCCCCA AGCCTCCAAT TTGGCTACTA GAATCTTGCT TTTAACACTT
    CTTTTTGAGG AATTGAAGCT ACCTTGTGCC TGGGTGGTTG AATCTAGTGG CATCCTTAAT
    GTCCTAATCA AACTCTTGGA AGTGGTTCAA CCCTGCCTAC AGGCCGCCAA GGAGCAAAAG
    GAGGTCCAGA CCCCAAAGTG GATCACCCCA GTGTTGCTCC TGATAGATTT CTATGAAAAG
    ACAGCCATCT CCTCTAAAAG GAGAGCCCAA ATGACTAAGT ATCTACAGTC CAACAACAAC
    AACTGGCGCT GGTTTGATGA CCGCTCTGGG CGTTGGTGTA GTTACAGTGC AAGCAACAAC
    AGCACTATTG ATTCTGCCTG GAAATCTGGA GAGACAAGTG TACGTTTCAC TGCAGGCCGA
    AGAAGATACA CTGTCCAGTT CACTACAATG GTGCAGGTTA ATGAAGAAAC CGGGAACCGG
    CGCCCTGTAA TGTTGACTCT CCTGAGGGTA CCACGGCTGA ATAAAAACTC GAAAAATAGC
    AATGGACAGG AACTAGAGAA GACACTGGAA GAAAGTAAAG AAATGGATAT CAAACGTAAA
    GAAAATAAAG CTAATGATAC TCCTTTGGCC CTAGAGAATA CAAATACTGA AAAGGAGACA
    AGCCTGGAAG AAACAAAAAT TGGGGAGATC CTGATCCAGG GCCTGACAGA GGATATGGTG
    ACTGTTTTAA TCCGAGCCTG CGTGAGCATG CTAGGAGTCC CTGTGGACCC AGACACTTTG
    CATGCCACCC TTCGTCTCTG CCTGAGGCTC ACCCGAGACC ACAAATACGC CATGATGTTT
    GCAGAGCTAA AGAGTACCCG CATGATCTTG AATTTGACCC AGAGCTCAGG CTTCAATGGG
    TTTACTCCCC TCGTCACCCT TCTCTTAAGA CACATCATTG AGGATCCCTG CACCCTCCGT
    CATACTATGG AAAAGGTGGT TCGCTCAGCA GCTACAAGTG GAGCTGGTAG CACTACCTCT
    GGCGTTGTAT CTGGCAGCCT TGGTTCTCGG GAGATCAACT ACATCCTTCG TGTCCTTGGG
    CCAGCTGCAT GCCGCAATCC AGACATTTTC ACAGAAGTAG CCAACTGCTG TATCCGCATT
    GCCCTTCCAG CCCCTCGAGG CTCAGGAACT GCTTCAGATG ATGAATTTGA GAATCTTAGA
    ATTAAAGGTC CTAATGCTGT ACAGCTGGTG AAGACAACCC CTTTGAAACC CTCGCCTCTG
    CCTGTCATTC CTGATACTAT CAAGGAAGTG ATCTATGATA TGCTGAATGC CCTGGCTGCA
    TACCATGCTC CAGAGGAAGC AGAGAAATCT GACCCTAAAC CTGGAGGTAT GACCCAAGAG
    GTTGGCCAAC TCCTACAAGA CATGGGTGAT GATGTATACC AACAGTACCG GTCACTTACT
    CGCCAGAGCA GTGACTTTGA TACCCAGTCA GGTTTTTCCA TTAATAGTCA AGTCTTTGCT
    GCAGATGGTG CCTCCACTGA GACTTCCACA TCTGGGACAT CCCAAGGAGA AGCATCAACT
    CCAGAGGAGT CTCGGGATGG GAAGAAAGAT AAAGAAGGGG ACCGGACCTC CGAGGAAGGC
    AAACAGAAAA GCAAGGGCAG CAAACCTTTA ATGCCCACTT CCACTATTCT TCGTCTTCTG
    GCAGAGTTGG TGAGGTCCTA TGTTGGTATT GCTACCCTGA TTGCCAACTA CAGCTACACT
    GTGGGCCAGT CTGAGCTGAT TAAAGAGGAC TGCAGTGTGC TAGCTTTTGT TCTGGACCAC
    CTCCTCCCAC ATACCCAGAA TGCAGAAGAC AAGGACACAC CTGCCCTGGC CCGCCTATTC
    CTCGCAAGCC TGGCTGCTGC AGGGAGTGGC ACAGATGCCC AAGTGGCCCT TGTGAATGAA
    GTAAAAGCAG CCCTGGGACG GGCACTGGCT ATGGCTGAGA GTACAGAGAA ACATGCCAGG
    CTTCAGGCAG TGATGTGTAT CATCAGTACT ATCATGGAGT CCTGCCCCTC CACCTCCAGC
    TTCTACAGCA GTGCCACAGC CAAGACCCAG CACAATGGCA TGAACAACAT CATTCGACTC
    TTTCTAAAGA AGGGACTGGT TAATGATCTG GCAAGAGTGC CTCACAGCCT GGACCTGTCC
    AGTCCAAACA TGGCCAACAC AGTCAATGCT GCTTTGAAGC CTTTGGAAAC ACTTTCCCGG
    ATTGTGAACC AGCCCAGTAG CCTTTTTGGC AGCAAGAGTG CTTCTAGCAA GAGCAAGTCT
    GAGCAGGATG CCCAAGGAGC TGCTCAGGAC TCCAATAGCA ACCAGCAAGA CCCAGGCGAG
    CCTGGGGAAG CAGAAGTGCA GGAGGAGGAT CATGATGTCA CTCAGACAGA GGTGGCAGAT
    GGGGATATCA TGGATGGGGA GGCTGAAACC GACTCAGTGG TGATTGCTGG GCAGCCTGAG
    GTGCTCAGTT CACAAGAGAT GCAGGTTGAG AATGAGCTGG AGGACCTGAT AGATGAGTTG
    CTTGAGAGGG ATGGCGGATC TGGGAACAGT ACAATTATAG CAGACATCCC CCCATCCCCA
    GGAAATATCC CTACCACTCA CCCACTGATG GTACGCCATG CAGACCACAG TTCTCTGACA
    CTGGGCAGTG GCTCCTCCAC AACACGTCTC ACCCAGGGCA TCGGGCGCAG TCAGAGGACC
    CTAAGGCAGC TGACAGCCAA TACAGGCCAC ACCATTCATG TTCATTACCC TGGGAACCGC
    CAGCCCAACC CTCCTCTTAT ACTCCAGAGG TTGCTTGGTC CTTCTGCTGC TGCTGACATC
    CTTCAGCTGA GCAGCAGCCT TCCCCTACAA AGCCGTGGAC GAGCCCGCCT CCTGGTAGGC
    AATGATGATG TCCACATCAT TGCCAGATCT GATGATGAAC TGCTGGATGA CTTTTTCCAT
    GACCAGAGCA CAGCTACCAG CCAAGCAGGG ACCCTATCCA GCATCCCCAC AGCCCTGACC
    CGCTGGACAG AGGAATGCAA AGTTCTTGAT GCCGAGAGCA TGCATGACTG TGTTTCAGTT
    GTTAAAGTCC CCATTGTCAA TCACTTGGAA TTCCTGAGGG ATGAGGAGCT GGAAGAAAGG
    CGGGAGAAAC GCAGGAAACA ATTGGCTGAG GAAGAAACAA AGATAACTGA CAAAGGCAAA
    GAAGATAAGG AGAACAGGGA TCAGAGTGCA CAGTGTACTG CATCTAAAAC AAATGACTCC
    GCTGAACAGA ACCTCTCAGA TGGGACTCCT ATGCCTGACA GCTACCCAAC AACCCCATCT
    TCAACTGATG CAGCCACATC TGAGTCCAAG GATACCCTTG TCACTCTGCA GCCCTCACAA
    CAGCAACAAA CACTCCCACC CCCACCAGCC TTGGGAGAGA TTCCTCAAGA GCTACAGTCC
    CCGGCTGGAG AAGGGGGGAG CTCTACACAG CTGTTGATGC CTGTAGAGCC AGAGGAATTG
    GGTCCCACAA GGCCAAGTGG AGAAGCAGAA ACAACTCAGA TGGAGTTGTC CCCAGCTCCT
    ACTATAAGCA GAGATCTAAC CTCTCCATGT GACTTCCTCC TAGCCTCTCT TTCCCCAGAG
    AGGGCTGAAG ATTCTGATGC GCTGACAGCT GTCAGCAGTC AACTGGAAGG CTCTCCCATG
    GACACCAGTA GCCTGGCTTC CTGTACCCTA GAGGAGGCTG TGGGTGACAC CTCAGCAGCT
    GGCAGTTCTG AGCAGCCCAC AGCAGGCAGC TCCACTCCTG GGGATGCCCC AACTGTTGTG
    ACAGAAGTGC AAAGCAGGGG TGATGGGTCA GGTGAACCTA CCCAGCCCCC TGAAGACAGC
    TCTCCCCCTG CATCCTCTGA GAGTTCTTCC ACCAGAGATT CTGCTGTGGC CATTTCTGGA
    GCAGATTCCC GAGGAATCCT AGAAGAGCCA CTGCCTTCAA CAAGCAGTGA GGAAGAAGAT
    CCCCTTGCAG GTATCAGTCT ACCAGAAGGT GTGGACCCCT CTTTCCTGGC TGCCCTGCCT
    GATGACATCC GCCGGGAAGT CCTACAGAAC CAACTGGGCA TCCGTCCACC AACTCGGACT
    GCCCCTTCGG CAAATAGCTC AGCTCCTGCA GTGGTGGGGA ATCCTGGTGT GACCGAAGTG
    AGCCCTGAGT TCCTGGCGGC CCTGCCTCCA GCCATTCAGG AGGAAGTGCT GGCACAGCAG
    AGAGCTGAGC AACAACGACG GGAACTGGCA CAGAATGCGA GCTCAGACAC TCCCATGGAC
    CCTGTGACCT TCATCCAGAC CCTGCCATCA GACTTGCGCC GTAGTGTCCT GGAGGACATG
    GAGGACAGTG TTTTGGCTGT GATGCCACCT GACATTGCAG CTGAGGCTCA AGCCCTGAGG
    CGAGAGCAGG AAGCCCGGCA GCGACAGCTC ATGCATGAGC GGCTCTTTGG CCACAGCAGC
    ACCTCTGCGC TCTCTGCCAT CCTCCGAAGC CCGGCTTTCA CCAGTCGCCT CAGTGGCAAC
    CGAGGAGTCC AATACACTCG TCTTGCTGTG CAAAGAGGTG GCACCTTCCA GATGGGGGGT
    AGCAGCAGCC ATAACAGGCC TTCTGGCAGT AATGTGGATA CTCTTCTCCG CCTCCGAGGA
    CGGCTCCTCC TGGACCATGA AGCCCTATCC TGTCTCCTGG TCCTACTTTT CGTGGATGAG
    CCAAAGCTTA ATACTAGTCG TCTACACCGA GTACTGAGAA ATCTCTGCTA CCATGCCCAG
    ACCCGCCACT GGGTCATCCG CAGTCTGCTC TCCATTTTGC AGCGCAGCAG TGAAAGTGAG
    CTATGCATTG AAACACCCAA GCTCTCCTCA AGTGAGGACA AGGGCAAAAA GTCAAGCAAA
    AGCTGTGGGT CAAGCAGCCA TGAGAACCGG CCCCTGGACC TCCTACACAA GATGGAGTCT
    AAGAGCTCCA ACCAGCTTTC CTGGCTCTCA GTATCCATGG ATGCAGCCCT CGGCTGCAGG
    ACTAATATAT TCCAGATTCA ACGTTCAGGA GGTCGCAAAC ATACCGAGAA GCATGCAAGC
    AGTGGCTCCA CTGTCCACAT CCACCCCCAG GCTGCTCCTG TTGTCTGCAG ACATGTTCTA
    GACACACTCA TTCAATTGGC CAAGGTATTT CCCAGCCATT TTACACAGCA GCGGACCAAA
    GAAACAAACT GTGAGAGTGA TCGGGAGAGG GGCAGTAAGC AGGCCTGCAG CCCATGCTCT
    TCACAGTCCA CCAGCAGTGG GATTTGCACA GACTTCTGGG ACTTACTGGT TAAACTGGAC
    AACATGAATG TCAGCCGGAA AGGCAAGAAC TCCGTGAAGT CAGTGCCAGT GAGCGCTGGT
    GGTGAGGGGG AAACCTCCCC ATACAGCCTT GAGGCCTCTC CACTGGGGCA GCTCATGAAC
    ATGTTATCAC ACCCAGTCAT CCGCCGGAGC TCTCTCTTAA CTGAGAAACT CCTCAGACTC
    CTTTCTCTCA TCTCAATTGC TCTCCCAGAA AACAAAGTGT CGGAAGCACA GGCTAATTCT
    GGCAGCAGTG CTTCCTCCAC CACTGTTGCC ACCTCAACCA CATCTACCAC CACTACCACT
    GCTGCCTCCA GCACGCCCAC TCCCCCTGCT GCAACCACCC CTGTCACTTC TGCTCCAGCT
    CTGGTTGCTG CCACGGCTAT ATCCACCATT GCCGTAGCTG CTTCGACCAC GGTGACTACC
    CCCACGACTG CTACCACTAC TGTTTCAACT TCTACAACTA CTAAGGCCAG CAAATCTCCA
    GCAAAGGTGG GTGATGGGGG CAGCAGCAGT GCTGACTTTA AGATGGTATC CTCTGGCCTC
    ACTGAAAACC AGCTGCAGCT CTCTGTGGAG GTGTTGACAT CCCACTCTTG TTCCGAAGAA
    GGTCTAGAGG ATGCAGCCAA TGTGTTACTG CAGCTCTCTC GGGGGGACCC TGCAACCCGG
    GACACTGTTC TCAAGCTGCT ACTGAATGGA GCGCGCCATC TGGGTTATAC TCTTTGTAAA
    CAGATAGGTA CCCTTCTGGC TGAGTTACGG GAATACAACC TAGAACAGCA GCGACGAGCC
    CAATGTGAGA CCCTCTCTCC TGATGGCCTG CCTGAGGAGC AGCCACAGAC CACCAAGCTA
    AAAGGCAAAA TGCAGAGCAG GTTTGACATG GCTGAGAATG TGGTAATTGT GGCATCTCAG
    AAGCGACCTC TGGGTGGCCG GGAGCTCCAG CTGCCTTCTA TGTCCATGTT GACATCCAAG
    ACGTCTACCC AGAAGTTCTT CTTGAGGGTA CTGCAGGTCA TCATCCAGCT TCGGGACGAC
    ACACGCCGAG CTAACAAGAA AGCCAAGCAG ACAGGCAGGC TAGGTTCTTC CGGTTTAGGC
    TCAGCTAGCA GCATCCAGGC AGCTGTTCGG CAGCTGGAGG CTGAGGCTGA TGCCATTATA
    CAAATGGTAC GTGAGGGTCA AAGGGCGCGG AGACAGCAAC AAGCAGCAAC GTCGGAGTCA
    AGCCAGTCAG AGGCCTCTGT CCGGAGAGAG GAGTCACCCA TGGATGTGGA CCAGCCATCT
    CCCAGTGCTC AGGATACTCA GTCCATTGGC TCAGATGGAA CCCCACAAGG GGAAAAGGAG
    AAGGAGGAGA GGCCACCTGA GTTACCCCTG CTCAGTGAGC AGCTGAGCTT GGACGAGCTG
    TGGGACATGC TGGGAGAGTG TCTAAAGGAA CTGGAGGAAT CCCATGACCA GCATGCAGTG
    CTAGTGCTAC AGCCTGCCGT CGAGGCCTTC TTTTTGGTCC ATGCCACAGA GCGGGAGAGC
    AAACCTCCTG TCAGAGACAC CCGTGAGAGC CAGCTGGCAC ACATCAAGGA CGAGCCTCCT
    CCACTCTCCC CTGCCCCCTT AACCCCTGCT ACTCCTTCCT CCCTTGACCC ATTCTTCTCC
    AGGGAGCCCT CCTCTATGCA CATCTCCTCA AGCCTGCCCC CTGATACACA GAAGTTCCTT
    CGCTTTGCAG AGACTCACCG CACTGTGTTA AATCAGATCC TACGGCAGTC CACCACCCAC
    CTCGCTGATG GGCCTTTTGC TGTCCTGGTA GACTACATTC GTGTCCTCGA CTTTGATGTC
    AAGCGCAAAT ATTTCCGCCA AGAGCTGGAA CGTCTGGATG AGGGGCTCCG GAAAGAAGAC
    ATGGCTGTGC ACGTCCGCCG TGATCATGTG TTTGAAGACT CCTATCGTGA ACTGCACCGG
    AAATCCCCTG AAGAAATGAA GAATCGATTG TATATAGTGT TTGAAGGAGA AGAAGGGCAG
    GATGCTGGAG GGCTCCTGCG GGAGTGGTAT ATGATCATCT CTCGGGAGAT GTTTAACCCT
    ATGTATGCCT TGTTCCGTAC CTCACCCGGT GATCGGGTCA CCTACACCAT CAACCCATCT
    TCCCACTGCA ACCCCAATCA CCTCAGCTAC TTCAAGTTTG TTGGACGCAT TGTGGCCAAA
    GCTGTATATG ACAACCGTCT CCTGGAGTGC TATTTTACTC GGTCCTTCTA CAAACACATC
    TTGGGCAAGT CTGTCAGATA TACAGATATG GAGAGTGAAG ATTACCACTT CTACCAGGGC
    CTGGTTTATC TGCTGGAAAA TGATGTCTCC ACACTAGGTT ATGACCTCAC CTTCAGCACT
    GAGGTCCAGG AGTTTGGAGT CTGTGAAGTT CGTGACCTCA AACCCAATGG GGCTAACATC
    TTGGTAACTG AAGAGAATAA GAAGGAATAT GTACACCTGG TGTGCCAGAT GAGGATGACA
    GGAGCCATCC GTAAACAGCT GGCAGCTTTC TTAGAAGGCT TCTATGAGAT CATTCCAAAG
    CGCCTCATTT CTATCTTCAC TGAGCAGGAG TTAGAGCTGC TCATATCAGG ACTGCCCACA
    ATCGACATTG ATGATTTGAA ATCCAACACT GAATACCACA AGTACCAGTC CAACTCTATT
    CAGATCCAGT GGTTCTGGAG AGCATTGCGT TCTTTCGACC AAGCTGACCG TGCCAAGTTC
    CTCCAATTTG TCACGGGTAC TTCAAAGGTG CCCTTACAAG GCTTTGCTGC CCTTGAAGGC
    ATGAATGGCA TTCAGAAGTT TCAGATCCAT CGGGATGACA GGTCCACAGA TCGCCTGCCT
    TCAGCTCACA CATGTTTTAA TCAGCTGGAC CTACCTGCCT ATGAGAGCTT TGAAAAGCTC
    CGCCACATGT TACTATTGGC CATCCAGGAG TGCTCTGAAG GCTTTGGGCT GGCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.