MIB2 cDNA ORF clone, Canis lupus familiaris(dog)

The following MIB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe06735 XM_014113813.1
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe06735 XM_005620389.3
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe06735 XM_014113815.1
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe06735 XM_022418904.1
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe06735 XM_014113814.1
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe06736 XM_022418905.1
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe06736 XM_005620394.2
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe06737 XM_022418906.1
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe06737 XM_014113817.1
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe06738 XM_014113818.1
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe06738 XM_022418907.1
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe06739 XM_022418908.1
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe06739 XM_005620393.2
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe36586 XM_014113812.2
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe36587 XM_014113816.2
Latest version!
Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
View Old Accession Versions >>
OCe36586 XM_014113812.1 Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe36587 XM_014113816.1 Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
OCe06735 XM_005620389.2 Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe06735
    Clone ID Related Accession (Same CDS sequence) XM_005620389.3 , XM_014113814.1 , XM_014113815.1 , XM_022418904.1 , XM_005620389.2 , XM_014113813.1
    Accession Version XM_005620389.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005620389.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACG
    GACCGTGGGG ATGTGCGTGT GCAGTTCGGC CGTGAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCACAATGC CTTCTGGGTG GGCGATGTGG TACGGGTCAT CGATGACCTT
    GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CAGATGACAT GGCCCCCGCC
    CTGGGCCGCA TTGGGAAGGT GGTGAAAGTT TTCAGAGACG GTAACCTGCG TGTGGCAGTC
    GGTGGTCAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAGGAT
    GCCAACCTGG ATGTGGCCGA ACGTGCCAGG GAGAACAAAA GCTTCCTGAG TGTTGCTCTG
    GAGAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGACTGGT GGTGGAGGTG
    GCCTTGGGCA GTATGGCTGG GGCTCTGGAC CAGCTGAGGC GGCACCCAGA GCAGGTGGAC
    ACCAAGAACC AGGGCAGGAC CGCTCTGCAG GTGGCCGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC ACGAGCGGGC ATGGACCTGG CAGATGATGA GGGCAATACG
    GCGCTGCACT ATGCAGCCTT GGGGAACCAG CCTGAGGCTG CCCGGTTGCT CCTGAGCTCC
    GGGTGTGGGG CCAATGCTCT TAACAGCACC CGGAGCGCAG CACTGCATGT GGCTGTGCAG
    AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAACGTG GCTGTGACGT CAACCTGCCT
    GATGCACACG CGGACACACC TCTGCACTGT GCCATCTCGG CGGGCGCCGG CGCCAGCGGC
    ATCGTGGAGG TCCTCACTGA GGTGCCAGGT GTTGATGTCA CGGCCACTAA CAGCCAGGGC
    TTCACCCTGC TGCATCACGC ATCCCTCAAG GGCCACACGC TAGCTGTCAG GAGGATTCTA
    GCTCGGGCAC GACAGCTGGT GGACGCCAAG AAGGAGGATG GCTTCACAGC CTTACACCTG
    GCTGCCCTCA ACAACCACCG GGAAGTGGCC CAGATTCTGA TCCGAGAGGG CCGCTGTGAT
    GTGAACGTTC GCAACCGTAA GCTCCAGTCC CCCCTGCACC TGGCCGTGCA GCAGGCTCAT
    GTGGGGCTGG TGCCGCTGCT AGTGGACGCG GGCTGCAGTG TCAACGCCGA GGATGAGGAA
    GGGGACACAG CCTTGCACGT GGCCCTGCAG CGTCATCAGC TGCTGCCCCT GGCAGCAGAT
    GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGCTCCAGGC CTCAGGCCTC
    CCGGGCAGCG GGGAGCTGAC GGTGGGAGCG GCGGTCGCAT GCTTCCTGGC GCTGGAGGGC
    GCCGACGTGA GCTACGCCAA CCACCGCGGC CGCAGCCCGC TGGACCTGGC CGCCGAGGGC
    CGTGTACTCA AGGCCCTGCA GGGCTGTGCG CAGCGCTTCC GGGAGAGGCA CGCGGGCGGC
    AGCGGGGGTG CGGCCCCGGG CCCGAGGCTG GTGCTTGGCA CCCCCAACAC CGTGACGAAC
    CTGCACGTGG CTGCGCCGTC GGGGCCCGAG GCCGCCGAGT GCCTGGTGTG CTCGGAGCTG
    GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACTG TGTGCGAGGA GTGCGCTCGC
    AGGATGAAGA AGTGCATCAG ATGCCAGGTG GTCATCGGCA AGAAGCTGCG ACCAGACGGC
    ACAGAGGTGG CCAGCGCGAC CCCCGCGTCC GGCCCGCCGC GGCAGCTGGT GGAGGAGCTG
    CAGAGTCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC
    ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCGTGCGCAC CCTGCGGCGC CGCGCTCAGC
    GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620446.1
    CDS510..3383
    Translation

    Target ORF information:

    RefSeq Version XM_005620389.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620389.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACG
    GACCGTGGGG ATGTGCGTGT GCAGTTCGGC CGTGAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCACAATGC CTTCTGGGTG GGCGATGTGG TACGGGTCAT CGATGACCTT
    GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CAGATGACAT GGCCCCCGCC
    CTGGGCCGCA TTGGGAAGGT GGTGAAAGTT TTCAGAGACG GTAACCTGCG TGTGGCAGTC
    GGTGGTCAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAGGAT
    GCCAACCTGG ATGTGGCCGA ACGTGCCAGG GAGAACAAAA GCTTCCTGAG TGTTGCTCTG
    GAGAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGACTGGT GGTGGAGGTG
    GCCTTGGGCA GTATGGCTGG GGCTCTGGAC CAGCTGAGGC GGCACCCAGA GCAGGTGGAC
    ACCAAGAACC AGGGCAGGAC CGCTCTGCAG GTGGCCGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC ACGAGCGGGC ATGGACCTGG CAGATGATGA GGGCAATACG
    GCGCTGCACT ATGCAGCCTT GGGGAACCAG CCTGAGGCTG CCCGGTTGCT CCTGAGCTCC
    GGGTGTGGGG CCAATGCTCT TAACAGCACC CGGAGCGCAG CACTGCATGT GGCTGTGCAG
    AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAACGTG GCTGTGACGT CAACCTGCCT
    GATGCACACG CGGACACACC TCTGCACTGT GCCATCTCGG CGGGCGCCGG CGCCAGCGGC
    ATCGTGGAGG TCCTCACTGA GGTGCCAGGT GTTGATGTCA CGGCCACTAA CAGCCAGGGC
    TTCACCCTGC TGCATCACGC ATCCCTCAAG GGCCACACGC TAGCTGTCAG GAGGATTCTA
    GCTCGGGCAC GACAGCTGGT GGACGCCAAG AAGGAGGATG GCTTCACAGC CTTACACCTG
    GCTGCCCTCA ACAACCACCG GGAAGTGGCC CAGATTCTGA TCCGAGAGGG CCGCTGTGAT
    GTGAACGTTC GCAACCGTAA GCTCCAGTCC CCCCTGCACC TGGCCGTGCA GCAGGCTCAT
    GTGGGGCTGG TGCCGCTGCT AGTGGACGCG GGCTGCAGTG TCAACGCCGA GGATGAGGAA
    GGGGACACAG CCTTGCACGT GGCCCTGCAG CGTCATCAGC TGCTGCCCCT GGCAGCAGAT
    GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGCTCCAGGC CTCAGGCCTC
    CCGGGCAGCG GGGAGCTGAC GGTGGGAGCG GCGGTCGCAT GCTTCCTGGC GCTGGAGGGC
    GCCGACGTGA GCTACGCCAA CCACCGCGGC CGCAGCCCGC TGGACCTGGC CGCCGAGGGC
    CGTGTACTCA AGGCCCTGCA GGGCTGTGCG CAGCGCTTCC GGGAGAGGCA CGCGGGCGGC
    AGCGGGGGTG CGGCCCCGGG CCCGAGGCTG GTGCTTGGCA CCCCCAACAC CGTGACGAAC
    CTGCACGTGG CTGCGCCGTC GGGGCCCGAG GCCGCCGAGT GCCTGGTGTG CTCGGAGCTG
    GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACTG TGTGCGAGGA GTGCGCTCGC
    AGGATGAAGA AGTGCATCAG ATGCCAGGTG GTCATCGGCA AGAAGCTGCG ACCAGACGGC
    ACAGAGGTGG CCAGCGCGAC CCCCGCGTCC GGCCCGCCGC GGCAGCTGGT GGAGGAGCTG
    CAGAGTCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC
    ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCGTGCGCAC CCTGCGGCGC CGCGCTCAGC
    GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06735
    Clone ID Related Accession (Same CDS sequence) XM_005620389.3 , XM_014113814.1 , XM_014113815.1 , XM_022418904.1 , XM_005620389.2 , XM_014113813.1
    Accession Version XM_014113814.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACG
    GACCGTGGGG ATGTGCGTGT GCAGTTCGGC CGTGAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCACAATGC CTTCTGGGTG GGCGATGTGG TACGGGTCAT CGATGACCTT
    GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CAGATGACAT GGCCCCCGCC
    CTGGGCCGCA TTGGGAAGGT GGTGAAAGTT TTCAGAGACG GTAACCTGCG TGTGGCAGTC
    GGTGGTCAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAGGAT
    GCCAACCTGG ATGTGGCCGA ACGTGCCAGG GAGAACAAAA GCTTCCTGAG TGTTGCTCTG
    GAGAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGACTGGT GGTGGAGGTG
    GCCTTGGGCA GTATGGCTGG GGCTCTGGAC CAGCTGAGGC GGCACCCAGA GCAGGTGGAC
    ACCAAGAACC AGGGCAGGAC CGCTCTGCAG GTGGCCGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC ACGAGCGGGC ATGGACCTGG CAGATGATGA GGGCAATACG
    GCGCTGCACT ATGCAGCCTT GGGGAACCAG CCTGAGGCTG CCCGGTTGCT CCTGAGCTCC
    GGGTGTGGGG CCAATGCTCT TAACAGCACC CGGAGCGCAG CACTGCATGT GGCTGTGCAG
    AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAACGTG GCTGTGACGT CAACCTGCCT
    GATGCACACG CGGACACACC TCTGCACTGT GCCATCTCGG CGGGCGCCGG CGCCAGCGGC
    ATCGTGGAGG TCCTCACTGA GGTGCCAGGT GTTGATGTCA CGGCCACTAA CAGCCAGGGC
    TTCACCCTGC TGCATCACGC ATCCCTCAAG GGCCACACGC TAGCTGTCAG GAGGATTCTA
    GCTCGGGCAC GACAGCTGGT GGACGCCAAG AAGGAGGATG GCTTCACAGC CTTACACCTG
    GCTGCCCTCA ACAACCACCG GGAAGTGGCC CAGATTCTGA TCCGAGAGGG CCGCTGTGAT
    GTGAACGTTC GCAACCGTAA GCTCCAGTCC CCCCTGCACC TGGCCGTGCA GCAGGCTCAT
    GTGGGGCTGG TGCCGCTGCT AGTGGACGCG GGCTGCAGTG TCAACGCCGA GGATGAGGAA
    GGGGACACAG CCTTGCACGT GGCCCTGCAG CGTCATCAGC TGCTGCCCCT GGCAGCAGAT
    GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGCTCCAGGC CTCAGGCCTC
    CCGGGCAGCG GGGAGCTGAC GGTGGGAGCG GCGGTCGCAT GCTTCCTGGC GCTGGAGGGC
    GCCGACGTGA GCTACGCCAA CCACCGCGGC CGCAGCCCGC TGGACCTGGC CGCCGAGGGC
    CGTGTACTCA AGGCCCTGCA GGGCTGTGCG CAGCGCTTCC GGGAGAGGCA CGCGGGCGGC
    AGCGGGGGTG CGGCCCCGGG CCCGAGGCTG GTGCTTGGCA CCCCCAACAC CGTGACGAAC
    CTGCACGTGG CTGCGCCGTC GGGGCCCGAG GCCGCCGAGT GCCTGGTGTG CTCGGAGCTG
    GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACTG TGTGCGAGGA GTGCGCTCGC
    AGGATGAAGA AGTGCATCAG ATGCCAGGTG GTCATCGGCA AGAAGCTGCG ACCAGACGGC
    ACAGAGGTGG CCAGCGCGAC CCCCGCGTCC GGCCCGCCGC GGCAGCTGGT GGAGGAGCTG
    CAGAGTCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC
    ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCGTGCGCAC CCTGCGGCGC CGCGCTCAGC
    GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969289.1
    CDS325..3198
    Translation

    Target ORF information:

    RefSeq Version XM_014113814.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113814.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACG
    GACCGTGGGG ATGTGCGTGT GCAGTTCGGC CGTGAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCACAATGC CTTCTGGGTG GGCGATGTGG TACGGGTCAT CGATGACCTT
    GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CAGATGACAT GGCCCCCGCC
    CTGGGCCGCA TTGGGAAGGT GGTGAAAGTT TTCAGAGACG GTAACCTGCG TGTGGCAGTC
    GGTGGTCAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAGGAT
    GCCAACCTGG ATGTGGCCGA ACGTGCCAGG GAGAACAAAA GCTTCCTGAG TGTTGCTCTG
    GAGAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGACTGGT GGTGGAGGTG
    GCCTTGGGCA GTATGGCTGG GGCTCTGGAC CAGCTGAGGC GGCACCCAGA GCAGGTGGAC
    ACCAAGAACC AGGGCAGGAC CGCTCTGCAG GTGGCCGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC ACGAGCGGGC ATGGACCTGG CAGATGATGA GGGCAATACG
    GCGCTGCACT ATGCAGCCTT GGGGAACCAG CCTGAGGCTG CCCGGTTGCT CCTGAGCTCC
    GGGTGTGGGG CCAATGCTCT TAACAGCACC CGGAGCGCAG CACTGCATGT GGCTGTGCAG
    AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAACGTG GCTGTGACGT CAACCTGCCT
    GATGCACACG CGGACACACC TCTGCACTGT GCCATCTCGG CGGGCGCCGG CGCCAGCGGC
    ATCGTGGAGG TCCTCACTGA GGTGCCAGGT GTTGATGTCA CGGCCACTAA CAGCCAGGGC
    TTCACCCTGC TGCATCACGC ATCCCTCAAG GGCCACACGC TAGCTGTCAG GAGGATTCTA
    GCTCGGGCAC GACAGCTGGT GGACGCCAAG AAGGAGGATG GCTTCACAGC CTTACACCTG
    GCTGCCCTCA ACAACCACCG GGAAGTGGCC CAGATTCTGA TCCGAGAGGG CCGCTGTGAT
    GTGAACGTTC GCAACCGTAA GCTCCAGTCC CCCCTGCACC TGGCCGTGCA GCAGGCTCAT
    GTGGGGCTGG TGCCGCTGCT AGTGGACGCG GGCTGCAGTG TCAACGCCGA GGATGAGGAA
    GGGGACACAG CCTTGCACGT GGCCCTGCAG CGTCATCAGC TGCTGCCCCT GGCAGCAGAT
    GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGCTCCAGGC CTCAGGCCTC
    CCGGGCAGCG GGGAGCTGAC GGTGGGAGCG GCGGTCGCAT GCTTCCTGGC GCTGGAGGGC
    GCCGACGTGA GCTACGCCAA CCACCGCGGC CGCAGCCCGC TGGACCTGGC CGCCGAGGGC
    CGTGTACTCA AGGCCCTGCA GGGCTGTGCG CAGCGCTTCC GGGAGAGGCA CGCGGGCGGC
    AGCGGGGGTG CGGCCCCGGG CCCGAGGCTG GTGCTTGGCA CCCCCAACAC CGTGACGAAC
    CTGCACGTGG CTGCGCCGTC GGGGCCCGAG GCCGCCGAGT GCCTGGTGTG CTCGGAGCTG
    GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACTG TGTGCGAGGA GTGCGCTCGC
    AGGATGAAGA AGTGCATCAG ATGCCAGGTG GTCATCGGCA AGAAGCTGCG ACCAGACGGC
    ACAGAGGTGG CCAGCGCGAC CCCCGCGTCC GGCCCGCCGC GGCAGCTGGT GGAGGAGCTG
    CAGAGTCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC
    ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCGTGCGCAC CCTGCGGCGC CGCGCTCAGC
    GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06735
    Clone ID Related Accession (Same CDS sequence) XM_005620389.3 , XM_014113814.1 , XM_014113815.1 , XM_022418904.1 , XM_005620389.2 , XM_014113813.1
    Accession Version XM_014113815.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACG
    GACCGTGGGG ATGTGCGTGT GCAGTTCGGC CGTGAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCACAATGC CTTCTGGGTG GGCGATGTGG TACGGGTCAT CGATGACCTT
    GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CAGATGACAT GGCCCCCGCC
    CTGGGCCGCA TTGGGAAGGT GGTGAAAGTT TTCAGAGACG GTAACCTGCG TGTGGCAGTC
    GGTGGTCAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAGGAT
    GCCAACCTGG ATGTGGCCGA ACGTGCCAGG GAGAACAAAA GCTTCCTGAG TGTTGCTCTG
    GAGAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGACTGGT GGTGGAGGTG
    GCCTTGGGCA GTATGGCTGG GGCTCTGGAC CAGCTGAGGC GGCACCCAGA GCAGGTGGAC
    ACCAAGAACC AGGGCAGGAC CGCTCTGCAG GTGGCCGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC ACGAGCGGGC ATGGACCTGG CAGATGATGA GGGCAATACG
    GCGCTGCACT ATGCAGCCTT GGGGAACCAG CCTGAGGCTG CCCGGTTGCT CCTGAGCTCC
    GGGTGTGGGG CCAATGCTCT TAACAGCACC CGGAGCGCAG CACTGCATGT GGCTGTGCAG
    AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAACGTG GCTGTGACGT CAACCTGCCT
    GATGCACACG CGGACACACC TCTGCACTGT GCCATCTCGG CGGGCGCCGG CGCCAGCGGC
    ATCGTGGAGG TCCTCACTGA GGTGCCAGGT GTTGATGTCA CGGCCACTAA CAGCCAGGGC
    TTCACCCTGC TGCATCACGC ATCCCTCAAG GGCCACACGC TAGCTGTCAG GAGGATTCTA
    GCTCGGGCAC GACAGCTGGT GGACGCCAAG AAGGAGGATG GCTTCACAGC CTTACACCTG
    GCTGCCCTCA ACAACCACCG GGAAGTGGCC CAGATTCTGA TCCGAGAGGG CCGCTGTGAT
    GTGAACGTTC GCAACCGTAA GCTCCAGTCC CCCCTGCACC TGGCCGTGCA GCAGGCTCAT
    GTGGGGCTGG TGCCGCTGCT AGTGGACGCG GGCTGCAGTG TCAACGCCGA GGATGAGGAA
    GGGGACACAG CCTTGCACGT GGCCCTGCAG CGTCATCAGC TGCTGCCCCT GGCAGCAGAT
    GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGCTCCAGGC CTCAGGCCTC
    CCGGGCAGCG GGGAGCTGAC GGTGGGAGCG GCGGTCGCAT GCTTCCTGGC GCTGGAGGGC
    GCCGACGTGA GCTACGCCAA CCACCGCGGC CGCAGCCCGC TGGACCTGGC CGCCGAGGGC
    CGTGTACTCA AGGCCCTGCA GGGCTGTGCG CAGCGCTTCC GGGAGAGGCA CGCGGGCGGC
    AGCGGGGGTG CGGCCCCGGG CCCGAGGCTG GTGCTTGGCA CCCCCAACAC CGTGACGAAC
    CTGCACGTGG CTGCGCCGTC GGGGCCCGAG GCCGCCGAGT GCCTGGTGTG CTCGGAGCTG
    GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACTG TGTGCGAGGA GTGCGCTCGC
    AGGATGAAGA AGTGCATCAG ATGCCAGGTG GTCATCGGCA AGAAGCTGCG ACCAGACGGC
    ACAGAGGTGG CCAGCGCGAC CCCCGCGTCC GGCCCGCCGC GGCAGCTGGT GGAGGAGCTG
    CAGAGTCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC
    ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCGTGCGCAC CCTGCGGCGC CGCGCTCAGC
    GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969290.1
    CDS61..2934
    Translation

    Target ORF information:

    RefSeq Version XM_014113815.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113815.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACG
    GACCGTGGGG ATGTGCGTGT GCAGTTCGGC CGTGAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCACAATGC CTTCTGGGTG GGCGATGTGG TACGGGTCAT CGATGACCTT
    GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CAGATGACAT GGCCCCCGCC
    CTGGGCCGCA TTGGGAAGGT GGTGAAAGTT TTCAGAGACG GTAACCTGCG TGTGGCAGTC
    GGTGGTCAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAGGAT
    GCCAACCTGG ATGTGGCCGA ACGTGCCAGG GAGAACAAAA GCTTCCTGAG TGTTGCTCTG
    GAGAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGACTGGT GGTGGAGGTG
    GCCTTGGGCA GTATGGCTGG GGCTCTGGAC CAGCTGAGGC GGCACCCAGA GCAGGTGGAC
    ACCAAGAACC AGGGCAGGAC CGCTCTGCAG GTGGCCGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC ACGAGCGGGC ATGGACCTGG CAGATGATGA GGGCAATACG
    GCGCTGCACT ATGCAGCCTT GGGGAACCAG CCTGAGGCTG CCCGGTTGCT CCTGAGCTCC
    GGGTGTGGGG CCAATGCTCT TAACAGCACC CGGAGCGCAG CACTGCATGT GGCTGTGCAG
    AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAACGTG GCTGTGACGT CAACCTGCCT
    GATGCACACG CGGACACACC TCTGCACTGT GCCATCTCGG CGGGCGCCGG CGCCAGCGGC
    ATCGTGGAGG TCCTCACTGA GGTGCCAGGT GTTGATGTCA CGGCCACTAA CAGCCAGGGC
    TTCACCCTGC TGCATCACGC ATCCCTCAAG GGCCACACGC TAGCTGTCAG GAGGATTCTA
    GCTCGGGCAC GACAGCTGGT GGACGCCAAG AAGGAGGATG GCTTCACAGC CTTACACCTG
    GCTGCCCTCA ACAACCACCG GGAAGTGGCC CAGATTCTGA TCCGAGAGGG CCGCTGTGAT
    GTGAACGTTC GCAACCGTAA GCTCCAGTCC CCCCTGCACC TGGCCGTGCA GCAGGCTCAT
    GTGGGGCTGG TGCCGCTGCT AGTGGACGCG GGCTGCAGTG TCAACGCCGA GGATGAGGAA
    GGGGACACAG CCTTGCACGT GGCCCTGCAG CGTCATCAGC TGCTGCCCCT GGCAGCAGAT
    GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGCTCCAGGC CTCAGGCCTC
    CCGGGCAGCG GGGAGCTGAC GGTGGGAGCG GCGGTCGCAT GCTTCCTGGC GCTGGAGGGC
    GCCGACGTGA GCTACGCCAA CCACCGCGGC CGCAGCCCGC TGGACCTGGC CGCCGAGGGC
    CGTGTACTCA AGGCCCTGCA GGGCTGTGCG CAGCGCTTCC GGGAGAGGCA CGCGGGCGGC
    AGCGGGGGTG CGGCCCCGGG CCCGAGGCTG GTGCTTGGCA CCCCCAACAC CGTGACGAAC
    CTGCACGTGG CTGCGCCGTC GGGGCCCGAG GCCGCCGAGT GCCTGGTGTG CTCGGAGCTG
    GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACTG TGTGCGAGGA GTGCGCTCGC
    AGGATGAAGA AGTGCATCAG ATGCCAGGTG GTCATCGGCA AGAAGCTGCG ACCAGACGGC
    ACAGAGGTGG CCAGCGCGAC CCCCGCGTCC GGCCCGCCGC GGCAGCTGGT GGAGGAGCTG
    CAGAGTCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC
    ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCGTGCGCAC CCTGCGGCGC CGCGCTCAGC
    GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06735
    Clone ID Related Accession (Same CDS sequence) XM_005620389.3 , XM_014113814.1 , XM_014113815.1 , XM_022418904.1 , XM_005620389.2 , XM_014113813.1
    Accession Version XM_022418904.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACG
    GACCGTGGGG ATGTGCGTGT GCAGTTCGGC CGTGAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCACAATGC CTTCTGGGTG GGCGATGTGG TACGGGTCAT CGATGACCTT
    GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CAGATGACAT GGCCCCCGCC
    CTGGGCCGCA TTGGGAAGGT GGTGAAAGTT TTCAGAGACG GTAACCTGCG TGTGGCAGTC
    GGTGGTCAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAGGAT
    GCCAACCTGG ATGTGGCCGA ACGTGCCAGG GAGAACAAAA GCTTCCTGAG TGTTGCTCTG
    GAGAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGACTGGT GGTGGAGGTG
    GCCTTGGGCA GTATGGCTGG GGCTCTGGAC CAGCTGAGGC GGCACCCAGA GCAGGTGGAC
    ACCAAGAACC AGGGCAGGAC CGCTCTGCAG GTGGCCGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC ACGAGCGGGC ATGGACCTGG CAGATGATGA GGGCAATACG
    GCGCTGCACT ATGCAGCCTT GGGGAACCAG CCTGAGGCTG CCCGGTTGCT CCTGAGCTCC
    GGGTGTGGGG CCAATGCTCT TAACAGCACC CGGAGCGCAG CACTGCATGT GGCTGTGCAG
    AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAACGTG GCTGTGACGT CAACCTGCCT
    GATGCACACG CGGACACACC TCTGCACTGT GCCATCTCGG CGGGCGCCGG CGCCAGCGGC
    ATCGTGGAGG TCCTCACTGA GGTGCCAGGT GTTGATGTCA CGGCCACTAA CAGCCAGGGC
    TTCACCCTGC TGCATCACGC ATCCCTCAAG GGCCACACGC TAGCTGTCAG GAGGATTCTA
    GCTCGGGCAC GACAGCTGGT GGACGCCAAG AAGGAGGATG GCTTCACAGC CTTACACCTG
    GCTGCCCTCA ACAACCACCG GGAAGTGGCC CAGATTCTGA TCCGAGAGGG CCGCTGTGAT
    GTGAACGTTC GCAACCGTAA GCTCCAGTCC CCCCTGCACC TGGCCGTGCA GCAGGCTCAT
    GTGGGGCTGG TGCCGCTGCT AGTGGACGCG GGCTGCAGTG TCAACGCCGA GGATGAGGAA
    GGGGACACAG CCTTGCACGT GGCCCTGCAG CGTCATCAGC TGCTGCCCCT GGCAGCAGAT
    GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGCTCCAGGC CTCAGGCCTC
    CCGGGCAGCG GGGAGCTGAC GGTGGGAGCG GCGGTCGCAT GCTTCCTGGC GCTGGAGGGC
    GCCGACGTGA GCTACGCCAA CCACCGCGGC CGCAGCCCGC TGGACCTGGC CGCCGAGGGC
    CGTGTACTCA AGGCCCTGCA GGGCTGTGCG CAGCGCTTCC GGGAGAGGCA CGCGGGCGGC
    AGCGGGGGTG CGGCCCCGGG CCCGAGGCTG GTGCTTGGCA CCCCCAACAC CGTGACGAAC
    CTGCACGTGG CTGCGCCGTC GGGGCCCGAG GCCGCCGAGT GCCTGGTGTG CTCGGAGCTG
    GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACTG TGTGCGAGGA GTGCGCTCGC
    AGGATGAAGA AGTGCATCAG ATGCCAGGTG GTCATCGGCA AGAAGCTGCG ACCAGACGGC
    ACAGAGGTGG CCAGCGCGAC CCCCGCGTCC GGCCCGCCGC GGCAGCTGGT GGAGGAGCTG
    CAGAGTCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC
    ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCGTGCGCAC CCTGCGGCGC CGCGCTCAGC
    GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274612.1
    CDS178..3051
    Translation

    Target ORF information:

    RefSeq Version XM_022418904.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418904.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACG
    GACCGTGGGG ATGTGCGTGT GCAGTTCGGC CGTGAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCACAATGC CTTCTGGGTG GGCGATGTGG TACGGGTCAT CGATGACCTT
    GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CAGATGACAT GGCCCCCGCC
    CTGGGCCGCA TTGGGAAGGT GGTGAAAGTT TTCAGAGACG GTAACCTGCG TGTGGCAGTC
    GGTGGTCAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAGGAT
    GCCAACCTGG ATGTGGCCGA ACGTGCCAGG GAGAACAAAA GCTTCCTGAG TGTTGCTCTG
    GAGAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGACTGGT GGTGGAGGTG
    GCCTTGGGCA GTATGGCTGG GGCTCTGGAC CAGCTGAGGC GGCACCCAGA GCAGGTGGAC
    ACCAAGAACC AGGGCAGGAC CGCTCTGCAG GTGGCCGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC ACGAGCGGGC ATGGACCTGG CAGATGATGA GGGCAATACG
    GCGCTGCACT ATGCAGCCTT GGGGAACCAG CCTGAGGCTG CCCGGTTGCT CCTGAGCTCC
    GGGTGTGGGG CCAATGCTCT TAACAGCACC CGGAGCGCAG CACTGCATGT GGCTGTGCAG
    AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAACGTG GCTGTGACGT CAACCTGCCT
    GATGCACACG CGGACACACC TCTGCACTGT GCCATCTCGG CGGGCGCCGG CGCCAGCGGC
    ATCGTGGAGG TCCTCACTGA GGTGCCAGGT GTTGATGTCA CGGCCACTAA CAGCCAGGGC
    TTCACCCTGC TGCATCACGC ATCCCTCAAG GGCCACACGC TAGCTGTCAG GAGGATTCTA
    GCTCGGGCAC GACAGCTGGT GGACGCCAAG AAGGAGGATG GCTTCACAGC CTTACACCTG
    GCTGCCCTCA ACAACCACCG GGAAGTGGCC CAGATTCTGA TCCGAGAGGG CCGCTGTGAT
    GTGAACGTTC GCAACCGTAA GCTCCAGTCC CCCCTGCACC TGGCCGTGCA GCAGGCTCAT
    GTGGGGCTGG TGCCGCTGCT AGTGGACGCG GGCTGCAGTG TCAACGCCGA GGATGAGGAA
    GGGGACACAG CCTTGCACGT GGCCCTGCAG CGTCATCAGC TGCTGCCCCT GGCAGCAGAT
    GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGCTCCAGGC CTCAGGCCTC
    CCGGGCAGCG GGGAGCTGAC GGTGGGAGCG GCGGTCGCAT GCTTCCTGGC GCTGGAGGGC
    GCCGACGTGA GCTACGCCAA CCACCGCGGC CGCAGCCCGC TGGACCTGGC CGCCGAGGGC
    CGTGTACTCA AGGCCCTGCA GGGCTGTGCG CAGCGCTTCC GGGAGAGGCA CGCGGGCGGC
    AGCGGGGGTG CGGCCCCGGG CCCGAGGCTG GTGCTTGGCA CCCCCAACAC CGTGACGAAC
    CTGCACGTGG CTGCGCCGTC GGGGCCCGAG GCCGCCGAGT GCCTGGTGTG CTCGGAGCTG
    GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACTG TGTGCGAGGA GTGCGCTCGC
    AGGATGAAGA AGTGCATCAG ATGCCAGGTG GTCATCGGCA AGAAGCTGCG ACCAGACGGC
    ACAGAGGTGG CCAGCGCGAC CCCCGCGTCC GGCCCGCCGC GGCAGCTGGT GGAGGAGCTG
    CAGAGTCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC
    ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCGTGCGCAC CCTGCGGCGC CGCGCTCAGC
    GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06735
    Clone ID Related Accession (Same CDS sequence) XM_005620389.3 , XM_014113814.1 , XM_014113815.1 , XM_022418904.1 , XM_005620389.2 , XM_014113813.1
    Accession Version XM_005620389.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620389.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACG
    GACCGTGGGG ATGTGCGTGT GCAGTTCGGC CGTGAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCACAATGC CTTCTGGGTG GGCGATGTGG TACGGGTCAT CGATGACCTT
    GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CAGATGACAT GGCCCCCGCC
    CTGGGCCGCA TTGGGAAGGT GGTGAAAGTT TTCAGAGACG GTAACCTGCG TGTGGCAGTC
    GGTGGTCAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAGGAT
    GCCAACCTGG ATGTGGCCGA ACGTGCCAGG GAGAACAAAA GCTTCCTGAG TGTTGCTCTG
    GAGAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGACTGGT GGTGGAGGTG
    GCCTTGGGCA GTATGGCTGG GGCTCTGGAC CAGCTGAGGC GGCACCCAGA GCAGGTGGAC
    ACCAAGAACC AGGGCAGGAC CGCTCTGCAG GTGGCCGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC ACGAGCGGGC ATGGACCTGG CAGATGATGA GGGCAATACG
    GCGCTGCACT ATGCAGCCTT GGGGAACCAG CCTGAGGCTG CCCGGTTGCT CCTGAGCTCC
    GGGTGTGGGG CCAATGCTCT TAACAGCACC CGGAGCGCAG CACTGCATGT GGCTGTGCAG
    AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAACGTG GCTGTGACGT CAACCTGCCT
    GATGCACACG CGGACACACC TCTGCACTGT GCCATCTCGG CGGGCGCCGG CGCCAGCGGC
    ATCGTGGAGG TCCTCACTGA GGTGCCAGGT GTTGATGTCA CGGCCACTAA CAGCCAGGGC
    TTCACCCTGC TGCATCACGC ATCCCTCAAG GGCCACACGC TAGCTGTCAG GAGGATTCTA
    GCTCGGGCAC GACAGCTGGT GGACGCCAAG AAGGAGGATG GCTTCACAGC CTTACACCTG
    GCTGCCCTCA ACAACCACCG GGAAGTGGCC CAGATTCTGA TCCGAGAGGG CCGCTGTGAT
    GTGAACGTTC GCAACCGTAA GCTCCAGTCC CCCCTGCACC TGGCCGTGCA GCAGGCTCAT
    GTGGGGCTGG TGCCGCTGCT AGTGGACGCG GGCTGCAGTG TCAACGCCGA GGATGAGGAA
    GGGGACACAG CCTTGCACGT GGCCCTGCAG CGTCATCAGC TGCTGCCCCT GGCAGCAGAT
    GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGCTCCAGGC CTCAGGCCTC
    CCGGGCAGCG GGGAGCTGAC GGTGGGAGCG GCGGTCGCAT GCTTCCTGGC GCTGGAGGGC
    GCCGACGTGA GCTACGCCAA CCACCGCGGC CGCAGCCCGC TGGACCTGGC CGCCGAGGGC
    CGTGTACTCA AGGCCCTGCA GGGCTGTGCG CAGCGCTTCC GGGAGAGGCA CGCGGGCGGC
    AGCGGGGGTG CGGCCCCGGG CCCGAGGCTG GTGCTTGGCA CCCCCAACAC CGTGACGAAC
    CTGCACGTGG CTGCGCCGTC GGGGCCCGAG GCCGCCGAGT GCCTGGTGTG CTCGGAGCTG
    GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACTG TGTGCGAGGA GTGCGCTCGC
    AGGATGAAGA AGTGCATCAG ATGCCAGGTG GTCATCGGCA AGAAGCTGCG ACCAGACGGC
    ACAGAGGTGG CCAGCGCGAC CCCCGCGTCC GGCCCGCCGC GGCAGCTGGT GGAGGAGCTG
    CAGAGTCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC
    ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCGTGCGCAC CCTGCGGCGC CGCGCTCAGC
    GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620446.1
    CDS2101..4974
    Translation

    Target ORF information:

    RefSeq Version XM_005620389.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620389.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACG
    GACCGTGGGG ATGTGCGTGT GCAGTTCGGC CGTGAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCACAATGC CTTCTGGGTG GGCGATGTGG TACGGGTCAT CGATGACCTT
    GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CAGATGACAT GGCCCCCGCC
    CTGGGCCGCA TTGGGAAGGT GGTGAAAGTT TTCAGAGACG GTAACCTGCG TGTGGCAGTC
    GGTGGTCAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAGGAT
    GCCAACCTGG ATGTGGCCGA ACGTGCCAGG GAGAACAAAA GCTTCCTGAG TGTTGCTCTG
    GAGAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGACTGGT GGTGGAGGTG
    GCCTTGGGCA GTATGGCTGG GGCTCTGGAC CAGCTGAGGC GGCACCCAGA GCAGGTGGAC
    ACCAAGAACC AGGGCAGGAC CGCTCTGCAG GTGGCCGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC ACGAGCGGGC ATGGACCTGG CAGATGATGA GGGCAATACG
    GCGCTGCACT ATGCAGCCTT GGGGAACCAG CCTGAGGCTG CCCGGTTGCT CCTGAGCTCC
    GGGTGTGGGG CCAATGCTCT TAACAGCACC CGGAGCGCAG CACTGCATGT GGCTGTGCAG
    AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAACGTG GCTGTGACGT CAACCTGCCT
    GATGCACACG CGGACACACC TCTGCACTGT GCCATCTCGG CGGGCGCCGG CGCCAGCGGC
    ATCGTGGAGG TCCTCACTGA GGTGCCAGGT GTTGATGTCA CGGCCACTAA CAGCCAGGGC
    TTCACCCTGC TGCATCACGC ATCCCTCAAG GGCCACACGC TAGCTGTCAG GAGGATTCTA
    GCTCGGGCAC GACAGCTGGT GGACGCCAAG AAGGAGGATG GCTTCACAGC CTTACACCTG
    GCTGCCCTCA ACAACCACCG GGAAGTGGCC CAGATTCTGA TCCGAGAGGG CCGCTGTGAT
    GTGAACGTTC GCAACCGTAA GCTCCAGTCC CCCCTGCACC TGGCCGTGCA GCAGGCTCAT
    GTGGGGCTGG TGCCGCTGCT AGTGGACGCG GGCTGCAGTG TCAACGCCGA GGATGAGGAA
    GGGGACACAG CCTTGCACGT GGCCCTGCAG CGTCATCAGC TGCTGCCCCT GGCAGCAGAT
    GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGCTCCAGGC CTCAGGCCTC
    CCGGGCAGCG GGGAGCTGAC GGTGGGAGCG GCGGTCGCAT GCTTCCTGGC GCTGGAGGGC
    GCCGACGTGA GCTACGCCAA CCACCGCGGC CGCAGCCCGC TGGACCTGGC CGCCGAGGGC
    CGTGTACTCA AGGCCCTGCA GGGCTGTGCG CAGCGCTTCC GGGAGAGGCA CGCGGGCGGC
    AGCGGGGGTG CGGCCCCGGG CCCGAGGCTG GTGCTTGGCA CCCCCAACAC CGTGACGAAC
    CTGCACGTGG CTGCGCCGTC GGGGCCCGAG GCCGCCGAGT GCCTGGTGTG CTCGGAGCTG
    GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACTG TGTGCGAGGA GTGCGCTCGC
    AGGATGAAGA AGTGCATCAG ATGCCAGGTG GTCATCGGCA AGAAGCTGCG ACCAGACGGC
    ACAGAGGTGG CCAGCGCGAC CCCCGCGTCC GGCCCGCCGC GGCAGCTGGT GGAGGAGCTG
    CAGAGTCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC
    ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCGTGCGCAC CCTGCGGCGC CGCGCTCAGC
    GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06735
    Clone ID Related Accession (Same CDS sequence) XM_005620389.3 , XM_014113814.1 , XM_014113815.1 , XM_022418904.1 , XM_005620389.2 , XM_014113813.1
    Accession Version XM_014113813.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2874bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACG
    GACCGTGGGG ATGTGCGTGT GCAGTTCGGC CGTGAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCACAATGC CTTCTGGGTG GGCGATGTGG TACGGGTCAT CGATGACCTT
    GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CAGATGACAT GGCCCCCGCC
    CTGGGCCGCA TTGGGAAGGT GGTGAAAGTT TTCAGAGACG GTAACCTGCG TGTGGCAGTC
    GGTGGTCAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAGGAT
    GCCAACCTGG ATGTGGCCGA ACGTGCCAGG GAGAACAAAA GCTTCCTGAG TGTTGCTCTG
    GAGAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGACTGGT GGTGGAGGTG
    GCCTTGGGCA GTATGGCTGG GGCTCTGGAC CAGCTGAGGC GGCACCCAGA GCAGGTGGAC
    ACCAAGAACC AGGGCAGGAC CGCTCTGCAG GTGGCCGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC ACGAGCGGGC ATGGACCTGG CAGATGATGA GGGCAATACG
    GCGCTGCACT ATGCAGCCTT GGGGAACCAG CCTGAGGCTG CCCGGTTGCT CCTGAGCTCC
    GGGTGTGGGG CCAATGCTCT TAACAGCACC CGGAGCGCAG CACTGCATGT GGCTGTGCAG
    AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAACGTG GCTGTGACGT CAACCTGCCT
    GATGCACACG CGGACACACC TCTGCACTGT GCCATCTCGG CGGGCGCCGG CGCCAGCGGC
    ATCGTGGAGG TCCTCACTGA GGTGCCAGGT GTTGATGTCA CGGCCACTAA CAGCCAGGGC
    TTCACCCTGC TGCATCACGC ATCCCTCAAG GGCCACACGC TAGCTGTCAG GAGGATTCTA
    GCTCGGGCAC GACAGCTGGT GGACGCCAAG AAGGAGGATG GCTTCACAGC CTTACACCTG
    GCTGCCCTCA ACAACCACCG GGAAGTGGCC CAGATTCTGA TCCGAGAGGG CCGCTGTGAT
    GTGAACGTTC GCAACCGTAA GCTCCAGTCC CCCCTGCACC TGGCCGTGCA GCAGGCTCAT
    GTGGGGCTGG TGCCGCTGCT AGTGGACGCG GGCTGCAGTG TCAACGCCGA GGATGAGGAA
    GGGGACACAG CCTTGCACGT GGCCCTGCAG CGTCATCAGC TGCTGCCCCT GGCAGCAGAT
    GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGCTCCAGGC CTCAGGCCTC
    CCGGGCAGCG GGGAGCTGAC GGTGGGAGCG GCGGTCGCAT GCTTCCTGGC GCTGGAGGGC
    GCCGACGTGA GCTACGCCAA CCACCGCGGC CGCAGCCCGC TGGACCTGGC CGCCGAGGGC
    CGTGTACTCA AGGCCCTGCA GGGCTGTGCG CAGCGCTTCC GGGAGAGGCA CGCGGGCGGC
    AGCGGGGGTG CGGCCCCGGG CCCGAGGCTG GTGCTTGGCA CCCCCAACAC CGTGACGAAC
    CTGCACGTGG CTGCGCCGTC GGGGCCCGAG GCCGCCGAGT GCCTGGTGTG CTCGGAGCTG
    GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACTG TGTGCGAGGA GTGCGCTCGC
    AGGATGAAGA AGTGCATCAG ATGCCAGGTG GTCATCGGCA AGAAGCTGCG ACCAGACGGC
    ACAGAGGTGG CCAGCGCGAC CCCCGCGTCC GGCCCGCCGC GGCAGCTGGT GGAGGAGCTG
    CAGAGTCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC
    ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCGTGCGCAC CCTGCGGCGC CGCGCTCAGC
    GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969288.1
    CDS420..3293
    Translation

    Target ORF information:

    RefSeq Version XM_014113813.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113813.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACG
    GACCGTGGGG ATGTGCGTGT GCAGTTCGGC CGTGAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCACAATGC CTTCTGGGTG GGCGATGTGG TACGGGTCAT CGATGACCTT
    GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CAGATGACAT GGCCCCCGCC
    CTGGGCCGCA TTGGGAAGGT GGTGAAAGTT TTCAGAGACG GTAACCTGCG TGTGGCAGTC
    GGTGGTCAGC TGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAGGAT
    GCCAACCTGG ATGTGGCCGA ACGTGCCAGG GAGAACAAAA GCTTCCTGAG TGTTGCTCTG
    GAGAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAGACTGGT GGTGGAGGTG
    GCCTTGGGCA GTATGGCTGG GGCTCTGGAC CAGCTGAGGC GGCACCCAGA GCAGGTGGAC
    ACCAAGAACC AGGGCAGGAC CGCTCTGCAG GTGGCCGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC ACGAGCGGGC ATGGACCTGG CAGATGATGA GGGCAATACG
    GCGCTGCACT ATGCAGCCTT GGGGAACCAG CCTGAGGCTG CCCGGTTGCT CCTGAGCTCC
    GGGTGTGGGG CCAATGCTCT TAACAGCACC CGGAGCGCAG CACTGCATGT GGCTGTGCAG
    AGGGGCTTCC TGGAGGTGGT GAAGGTCCTC TGTGAACGTG GCTGTGACGT CAACCTGCCT
    GATGCACACG CGGACACACC TCTGCACTGT GCCATCTCGG CGGGCGCCGG CGCCAGCGGC
    ATCGTGGAGG TCCTCACTGA GGTGCCAGGT GTTGATGTCA CGGCCACTAA CAGCCAGGGC
    TTCACCCTGC TGCATCACGC ATCCCTCAAG GGCCACACGC TAGCTGTCAG GAGGATTCTA
    GCTCGGGCAC GACAGCTGGT GGACGCCAAG AAGGAGGATG GCTTCACAGC CTTACACCTG
    GCTGCCCTCA ACAACCACCG GGAAGTGGCC CAGATTCTGA TCCGAGAGGG CCGCTGTGAT
    GTGAACGTTC GCAACCGTAA GCTCCAGTCC CCCCTGCACC TGGCCGTGCA GCAGGCTCAT
    GTGGGGCTGG TGCCGCTGCT AGTGGACGCG GGCTGCAGTG TCAACGCCGA GGATGAGGAA
    GGGGACACAG CCTTGCACGT GGCCCTGCAG CGTCATCAGC TGCTGCCCCT GGCAGCAGAT
    GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGCTCCAGGC CTCAGGCCTC
    CCGGGCAGCG GGGAGCTGAC GGTGGGAGCG GCGGTCGCAT GCTTCCTGGC GCTGGAGGGC
    GCCGACGTGA GCTACGCCAA CCACCGCGGC CGCAGCCCGC TGGACCTGGC CGCCGAGGGC
    CGTGTACTCA AGGCCCTGCA GGGCTGTGCG CAGCGCTTCC GGGAGAGGCA CGCGGGCGGC
    AGCGGGGGTG CGGCCCCGGG CCCGAGGCTG GTGCTTGGCA CCCCCAACAC CGTGACGAAC
    CTGCACGTGG CTGCGCCGTC GGGGCCCGAG GCCGCCGAGT GCCTGGTGTG CTCGGAGCTG
    GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACTG TGTGCGAGGA GTGCGCTCGC
    AGGATGAAGA AGTGCATCAG ATGCCAGGTG GTCATCGGCA AGAAGCTGCG ACCAGACGGC
    ACAGAGGTGG CCAGCGCGAC CCCCGCGTCC GGCCCGCCGC GGCAGCTGGT GGAGGAGCTG
    CAGAGTCGCT ACCGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC
    ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCGTGCGCAC CCTGCGGCGC CGCGCTCAGC
    GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06736
    Clone ID Related Accession (Same CDS sequence) XM_005620394.2 , XM_022418905.1
    Accession Version XM_005620394.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2862bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620394.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGACGGGC ACCGTGCACC GCATCACGGA CCGTGGGGAT
    GTGCGTGTGC AGTTCGGCCG TGAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG
    CACAATGCCT TCTGGGTGGG CGATGTGGTA CGGGTCATCG ATGACCTTGA CACAGTGAAG
    CGGCTGCAGG CTGGGCATGG CGAGTGGACA GATGACATGG CCCCCGCCCT GGGCCGCATT
    GGGAAGGTGG TGAAAGTTTT CAGAGACGGT AACCTGCGTG TGGCAGTCGG TGGTCAGCTG
    TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAGGATGC CAACCTGGAT
    GTGGCCGAAC GTGCCAGGGA GAACAAAAGC TTCCTGAGTG TTGCTCTGGA GAAGCTTCGA
    GCCCAGAAGA GTGACCTGGA GCACCCAGGG AGACTGGTGG TGGAGGTGGC CTTGGGCAGT
    ATGGCTGGGG CTCTGGACCA GCTGAGGCGG CACCCAGAGC AGGTGGACAC CAAGAACCAG
    GGCAGGACCG CTCTGCAGGT GGCCGCCTAC CTAGGCCAGG TGGAGCTGGT GCGGCTGCTG
    CTGCAGGCAC GAGCGGGCAT GGACCTGGCA GATGATGAGG GCAATACGGC GCTGCACTAT
    GCAGCCTTGG GGAACCAGCC TGAGGCTGCC CGGTTGCTCC TGAGCTCCGG GTGTGGGGCC
    AATGCTCTTA ACAGCACCCG GAGCGCAGCA CTGCATGTGG CTGTGCAGAG GGGCTTCCTG
    GAGGTGGTGA AGGTCCTCTG TGAACGTGGC TGTGACGTCA ACCTGCCTGA TGCACACGCG
    GACACACCTC TGCACTGTGC CATCTCGGCG GGCGCCGGCG CCAGCGGCAT CGTGGAGGTC
    CTCACTGAGG TGCCAGGTGT TGATGTCACG GCCACTAACA GCCAGGGCTT CACCCTGCTG
    CATCACGCAT CCCTCAAGGG CCACACGCTA GCTGTCAGGA GGATTCTAGC TCGGGCACGA
    CAGCTGGTGG ACGCCAAGAA GGAGGATGGC TTCACAGCCT TACACCTGGC TGCCCTCAAC
    AACCACCGGG AAGTGGCCCA GATTCTGATC CGAGAGGGCC GCTGTGATGT GAACGTTCGC
    AACCGTAAGC TCCAGTCCCC CCTGCACCTG GCCGTGCAGC AGGCTCATGT GGGGCTGGTG
    CCGCTGCTAG TGGACGCGGG CTGCAGTGTC AACGCCGAGG ATGAGGAAGG GGACACAGCC
    TTGCACGTGG CCCTGCAGCG TCATCAGCTG CTGCCCCTGG CAGCAGATGG GGCCGGGGGG
    GACCCAGGGC CCTTGCAGCT GCTGTCCAGG CTCCAGGCCT CAGGCCTCCC GGGCAGCGGG
    GAGCTGACGG TGGGAGCGGC GGTCGCATGC TTCCTGGCGC TGGAGGGCGC CGACGTGAGC
    TACGCCAACC ACCGCGGCCG CAGCCCGCTG GACCTGGCCG CCGAGGGCCG TGTACTCAAG
    GCCCTGCAGG GCTGTGCGCA GCGCTTCCGG GAGAGGCACG CGGGCGGCAG CGGGGGTGCG
    GCCCCGGGCC CGAGGCTGGT GCTTGGCACC CCCAACACCG TGACGAACCT GCACGTGGCT
    GCGCCGTCGG GGCCCGAGGC CGCCGAGTGC CTGGTGTGCT CGGAGCTGGC GCTGCTGGTG
    CTGTTCTCGC CGTGCCAGCA CCGCACTGTG TGCGAGGAGT GCGCTCGCAG GATGAAGAAG
    TGCATCAGAT GCCAGGTGGT CATCGGCAAG AAGCTGCGAC CAGACGGCAC AGAGGTGGCC
    AGCGCGACCC CCGCGTCCGG CCCGCCGCGG CAGCTGGTGG AGGAGCTGCA GAGTCGCTAC
    CGGCAGATGG AGGAGCGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG
    TTCCAGTGCG GCCACGGCGC GTGCGCACCC TGCGGCGCCG CGCTCAGCGC CTGCCCCATC
    TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620451.1
    CDS420..3281
    Translation

    Target ORF information:

    RefSeq Version XM_005620394.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620394.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGACGGGC ACCGTGCACC GCATCACGGA CCGTGGGGAT
    GTGCGTGTGC AGTTCGGCCG TGAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG
    CACAATGCCT TCTGGGTGGG CGATGTGGTA CGGGTCATCG ATGACCTTGA CACAGTGAAG
    CGGCTGCAGG CTGGGCATGG CGAGTGGACA GATGACATGG CCCCCGCCCT GGGCCGCATT
    GGGAAGGTGG TGAAAGTTTT CAGAGACGGT AACCTGCGTG TGGCAGTCGG TGGTCAGCTG
    TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAGGATGC CAACCTGGAT
    GTGGCCGAAC GTGCCAGGGA GAACAAAAGC TTCCTGAGTG TTGCTCTGGA GAAGCTTCGA
    GCCCAGAAGA GTGACCTGGA GCACCCAGGG AGACTGGTGG TGGAGGTGGC CTTGGGCAGT
    ATGGCTGGGG CTCTGGACCA GCTGAGGCGG CACCCAGAGC AGGTGGACAC CAAGAACCAG
    GGCAGGACCG CTCTGCAGGT GGCCGCCTAC CTAGGCCAGG TGGAGCTGGT GCGGCTGCTG
    CTGCAGGCAC GAGCGGGCAT GGACCTGGCA GATGATGAGG GCAATACGGC GCTGCACTAT
    GCAGCCTTGG GGAACCAGCC TGAGGCTGCC CGGTTGCTCC TGAGCTCCGG GTGTGGGGCC
    AATGCTCTTA ACAGCACCCG GAGCGCAGCA CTGCATGTGG CTGTGCAGAG GGGCTTCCTG
    GAGGTGGTGA AGGTCCTCTG TGAACGTGGC TGTGACGTCA ACCTGCCTGA TGCACACGCG
    GACACACCTC TGCACTGTGC CATCTCGGCG GGCGCCGGCG CCAGCGGCAT CGTGGAGGTC
    CTCACTGAGG TGCCAGGTGT TGATGTCACG GCCACTAACA GCCAGGGCTT CACCCTGCTG
    CATCACGCAT CCCTCAAGGG CCACACGCTA GCTGTCAGGA GGATTCTAGC TCGGGCACGA
    CAGCTGGTGG ACGCCAAGAA GGAGGATGGC TTCACAGCCT TACACCTGGC TGCCCTCAAC
    AACCACCGGG AAGTGGCCCA GATTCTGATC CGAGAGGGCC GCTGTGATGT GAACGTTCGC
    AACCGTAAGC TCCAGTCCCC CCTGCACCTG GCCGTGCAGC AGGCTCATGT GGGGCTGGTG
    CCGCTGCTAG TGGACGCGGG CTGCAGTGTC AACGCCGAGG ATGAGGAAGG GGACACAGCC
    TTGCACGTGG CCCTGCAGCG TCATCAGCTG CTGCCCCTGG CAGCAGATGG GGCCGGGGGG
    GACCCAGGGC CCTTGCAGCT GCTGTCCAGG CTCCAGGCCT CAGGCCTCCC GGGCAGCGGG
    GAGCTGACGG TGGGAGCGGC GGTCGCATGC TTCCTGGCGC TGGAGGGCGC CGACGTGAGC
    TACGCCAACC ACCGCGGCCG CAGCCCGCTG GACCTGGCCG CCGAGGGCCG TGTACTCAAG
    GCCCTGCAGG GCTGTGCGCA GCGCTTCCGG GAGAGGCACG CGGGCGGCAG CGGGGGTGCG
    GCCCCGGGCC CGAGGCTGGT GCTTGGCACC CCCAACACCG TGACGAACCT GCACGTGGCT
    GCGCCGTCGG GGCCCGAGGC CGCCGAGTGC CTGGTGTGCT CGGAGCTGGC GCTGCTGGTG
    CTGTTCTCGC CGTGCCAGCA CCGCACTGTG TGCGAGGAGT GCGCTCGCAG GATGAAGAAG
    TGCATCAGAT GCCAGGTGGT CATCGGCAAG AAGCTGCGAC CAGACGGCAC AGAGGTGGCC
    AGCGCGACCC CCGCGTCCGG CCCGCCGCGG CAGCTGGTGG AGGAGCTGCA GAGTCGCTAC
    CGGCAGATGG AGGAGCGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG
    TTCCAGTGCG GCCACGGCGC GTGCGCACCC TGCGGCGCCG CGCTCAGCGC CTGCCCCATC
    TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06736
    Clone ID Related Accession (Same CDS sequence) XM_005620394.2 , XM_022418905.1
    Accession Version XM_022418905.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2862bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGACGGGC ACCGTGCACC GCATCACGGA CCGTGGGGAT
    GTGCGTGTGC AGTTCGGCCG TGAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG
    CACAATGCCT TCTGGGTGGG CGATGTGGTA CGGGTCATCG ATGACCTTGA CACAGTGAAG
    CGGCTGCAGG CTGGGCATGG CGAGTGGACA GATGACATGG CCCCCGCCCT GGGCCGCATT
    GGGAAGGTGG TGAAAGTTTT CAGAGACGGT AACCTGCGTG TGGCAGTCGG TGGTCAGCTG
    TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAGGATGC CAACCTGGAT
    GTGGCCGAAC GTGCCAGGGA GAACAAAAGC TTCCTGAGTG TTGCTCTGGA GAAGCTTCGA
    GCCCAGAAGA GTGACCTGGA GCACCCAGGG AGACTGGTGG TGGAGGTGGC CTTGGGCAGT
    ATGGCTGGGG CTCTGGACCA GCTGAGGCGG CACCCAGAGC AGGTGGACAC CAAGAACCAG
    GGCAGGACCG CTCTGCAGGT GGCCGCCTAC CTAGGCCAGG TGGAGCTGGT GCGGCTGCTG
    CTGCAGGCAC GAGCGGGCAT GGACCTGGCA GATGATGAGG GCAATACGGC GCTGCACTAT
    GCAGCCTTGG GGAACCAGCC TGAGGCTGCC CGGTTGCTCC TGAGCTCCGG GTGTGGGGCC
    AATGCTCTTA ACAGCACCCG GAGCGCAGCA CTGCATGTGG CTGTGCAGAG GGGCTTCCTG
    GAGGTGGTGA AGGTCCTCTG TGAACGTGGC TGTGACGTCA ACCTGCCTGA TGCACACGCG
    GACACACCTC TGCACTGTGC CATCTCGGCG GGCGCCGGCG CCAGCGGCAT CGTGGAGGTC
    CTCACTGAGG TGCCAGGTGT TGATGTCACG GCCACTAACA GCCAGGGCTT CACCCTGCTG
    CATCACGCAT CCCTCAAGGG CCACACGCTA GCTGTCAGGA GGATTCTAGC TCGGGCACGA
    CAGCTGGTGG ACGCCAAGAA GGAGGATGGC TTCACAGCCT TACACCTGGC TGCCCTCAAC
    AACCACCGGG AAGTGGCCCA GATTCTGATC CGAGAGGGCC GCTGTGATGT GAACGTTCGC
    AACCGTAAGC TCCAGTCCCC CCTGCACCTG GCCGTGCAGC AGGCTCATGT GGGGCTGGTG
    CCGCTGCTAG TGGACGCGGG CTGCAGTGTC AACGCCGAGG ATGAGGAAGG GGACACAGCC
    TTGCACGTGG CCCTGCAGCG TCATCAGCTG CTGCCCCTGG CAGCAGATGG GGCCGGGGGG
    GACCCAGGGC CCTTGCAGCT GCTGTCCAGG CTCCAGGCCT CAGGCCTCCC GGGCAGCGGG
    GAGCTGACGG TGGGAGCGGC GGTCGCATGC TTCCTGGCGC TGGAGGGCGC CGACGTGAGC
    TACGCCAACC ACCGCGGCCG CAGCCCGCTG GACCTGGCCG CCGAGGGCCG TGTACTCAAG
    GCCCTGCAGG GCTGTGCGCA GCGCTTCCGG GAGAGGCACG CGGGCGGCAG CGGGGGTGCG
    GCCCCGGGCC CGAGGCTGGT GCTTGGCACC CCCAACACCG TGACGAACCT GCACGTGGCT
    GCGCCGTCGG GGCCCGAGGC CGCCGAGTGC CTGGTGTGCT CGGAGCTGGC GCTGCTGGTG
    CTGTTCTCGC CGTGCCAGCA CCGCACTGTG TGCGAGGAGT GCGCTCGCAG GATGAAGAAG
    TGCATCAGAT GCCAGGTGGT CATCGGCAAG AAGCTGCGAC CAGACGGCAC AGAGGTGGCC
    AGCGCGACCC CCGCGTCCGG CCCGCCGCGG CAGCTGGTGG AGGAGCTGCA GAGTCGCTAC
    CGGCAGATGG AGGAGCGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG
    TTCCAGTGCG GCCACGGCGC GTGCGCACCC TGCGGCGCCG CGCTCAGCGC CTGCCCCATC
    TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274613.1
    CDS510..3371
    Translation

    Target ORF information:

    RefSeq Version XM_022418905.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418905.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGACGGGC ACCGTGCACC GCATCACGGA CCGTGGGGAT
    GTGCGTGTGC AGTTCGGCCG TGAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG
    CACAATGCCT TCTGGGTGGG CGATGTGGTA CGGGTCATCG ATGACCTTGA CACAGTGAAG
    CGGCTGCAGG CTGGGCATGG CGAGTGGACA GATGACATGG CCCCCGCCCT GGGCCGCATT
    GGGAAGGTGG TGAAAGTTTT CAGAGACGGT AACCTGCGTG TGGCAGTCGG TGGTCAGCTG
    TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAGGATGC CAACCTGGAT
    GTGGCCGAAC GTGCCAGGGA GAACAAAAGC TTCCTGAGTG TTGCTCTGGA GAAGCTTCGA
    GCCCAGAAGA GTGACCTGGA GCACCCAGGG AGACTGGTGG TGGAGGTGGC CTTGGGCAGT
    ATGGCTGGGG CTCTGGACCA GCTGAGGCGG CACCCAGAGC AGGTGGACAC CAAGAACCAG
    GGCAGGACCG CTCTGCAGGT GGCCGCCTAC CTAGGCCAGG TGGAGCTGGT GCGGCTGCTG
    CTGCAGGCAC GAGCGGGCAT GGACCTGGCA GATGATGAGG GCAATACGGC GCTGCACTAT
    GCAGCCTTGG GGAACCAGCC TGAGGCTGCC CGGTTGCTCC TGAGCTCCGG GTGTGGGGCC
    AATGCTCTTA ACAGCACCCG GAGCGCAGCA CTGCATGTGG CTGTGCAGAG GGGCTTCCTG
    GAGGTGGTGA AGGTCCTCTG TGAACGTGGC TGTGACGTCA ACCTGCCTGA TGCACACGCG
    GACACACCTC TGCACTGTGC CATCTCGGCG GGCGCCGGCG CCAGCGGCAT CGTGGAGGTC
    CTCACTGAGG TGCCAGGTGT TGATGTCACG GCCACTAACA GCCAGGGCTT CACCCTGCTG
    CATCACGCAT CCCTCAAGGG CCACACGCTA GCTGTCAGGA GGATTCTAGC TCGGGCACGA
    CAGCTGGTGG ACGCCAAGAA GGAGGATGGC TTCACAGCCT TACACCTGGC TGCCCTCAAC
    AACCACCGGG AAGTGGCCCA GATTCTGATC CGAGAGGGCC GCTGTGATGT GAACGTTCGC
    AACCGTAAGC TCCAGTCCCC CCTGCACCTG GCCGTGCAGC AGGCTCATGT GGGGCTGGTG
    CCGCTGCTAG TGGACGCGGG CTGCAGTGTC AACGCCGAGG ATGAGGAAGG GGACACAGCC
    TTGCACGTGG CCCTGCAGCG TCATCAGCTG CTGCCCCTGG CAGCAGATGG GGCCGGGGGG
    GACCCAGGGC CCTTGCAGCT GCTGTCCAGG CTCCAGGCCT CAGGCCTCCC GGGCAGCGGG
    GAGCTGACGG TGGGAGCGGC GGTCGCATGC TTCCTGGCGC TGGAGGGCGC CGACGTGAGC
    TACGCCAACC ACCGCGGCCG CAGCCCGCTG GACCTGGCCG CCGAGGGCCG TGTACTCAAG
    GCCCTGCAGG GCTGTGCGCA GCGCTTCCGG GAGAGGCACG CGGGCGGCAG CGGGGGTGCG
    GCCCCGGGCC CGAGGCTGGT GCTTGGCACC CCCAACACCG TGACGAACCT GCACGTGGCT
    GCGCCGTCGG GGCCCGAGGC CGCCGAGTGC CTGGTGTGCT CGGAGCTGGC GCTGCTGGTG
    CTGTTCTCGC CGTGCCAGCA CCGCACTGTG TGCGAGGAGT GCGCTCGCAG GATGAAGAAG
    TGCATCAGAT GCCAGGTGGT CATCGGCAAG AAGCTGCGAC CAGACGGCAC AGAGGTGGCC
    AGCGCGACCC CCGCGTCCGG CCCGCCGCGG CAGCTGGTGG AGGAGCTGCA GAGTCGCTAC
    CGGCAGATGG AGGAGCGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG
    TTCCAGTGCG GCCACGGCGC GTGCGCACCC TGCGGCGCCG CGCTCAGCGC CTGCCCCATC
    TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06737
    Clone ID Related Accession (Same CDS sequence) XM_022418906.1 , XM_014113817.1
    Accession Version XM_022418906.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2847bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGCACAAT GCCTTCTGGG TGGGCGATGT GGTACGGGTC
    ATCGATGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACAGATGAC
    ATGGCCCCCG CCCTGGGCCG CATTGGGAAG GTGGTGAAAG TTTTCAGAGA CGGTAACCTG
    CGTGTGGCAG TCGGTGGTCA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAGG ATGCCAACCT GGATGTGGCC GAACGTGCCA GGGAGAACAA AAGTGGGTGT
    GCCCACCTTG GGTGGCCGGC AGGAGGCAGG GCTGCCTTTG GCCTCCACTT AACTGAAGCC
    CTGCCCCACC CAGGCTTCCT GAGTGTTGCT CTGGAGAAGC TTCGAGCCCA GAAGAGTGAC
    CTGGAGCACC CAGGGAGACT GGTGGTGGAG GTGGCCTTGG GCAGTATGGC TGGGGCTCTG
    GACCAGCTGA GGCGGCACCC AGAGCAGGTG GACACCAAGA ACCAGGGCAG GACCGCTCTG
    CAGGTGGCCG CCTACCTAGG CCAGGTGGAG CTGGTGCGGC TGCTGCTGCA GGCACGAGCG
    GGCATGGACC TGGCAGATGA TGAGGGCAAT ACGGCGCTGC ACTATGCAGC CTTGGGGAAC
    CAGCCTGAGG CTGCCCGGTT GCTCCTGAGC TCCGGGTGTG GGGCCAATGC TCTTAACAGC
    ACCCGGAGCG CAGCACTGCA TGTGGCTGTG CAGAGGGGCT TCCTGGAGGT GGTGAAGGTC
    CTCTGTGAAC GTGGCTGTGA CGTCAACCTG CCTGATGCAC ACGCGGACAC ACCTCTGCAC
    TGTGCCATCT CGGCGGGCGC CGGCGCCAGC GGCATCGTGG AGGTCCTCAC TGAGGTGCCA
    GGTGTTGATG TCACGGCCAC TAACAGCCAG GGCTTCACCC TGCTGCATCA CGCATCCCTC
    AAGGGCCACA CGCTAGCTGT CAGGAGGATT CTAGCTCGGG CACGACAGCT GGTGGACGCC
    AAGAAGGAGG ATGGCTTCAC AGCCTTACAC CTGGCTGCCC TCAACAACCA CCGGGAAGTG
    GCCCAGATTC TGATCCGAGA GGGCCGCTGT GATGTGAACG TTCGCAACCG TAAGCTCCAG
    TCCCCCCTGC ACCTGGCCGT GCAGCAGGCT CATGTGGGGC TGGTGCCGCT GCTAGTGGAC
    GCGGGCTGCA GTGTCAACGC CGAGGATGAG GAAGGGGACA CAGCCTTGCA CGTGGCCCTG
    CAGCGTCATC AGCTGCTGCC CCTGGCAGCA GATGGGGCCG GGGGGGACCC AGGGCCCTTG
    CAGCTGCTGT CCAGGCTCCA GGCCTCAGGC CTCCCGGGCA GCGGGGAGCT GACGGTGGGA
    GCGGCGGTCG CATGCTTCCT GGCGCTGGAG GGCGCCGACG TGAGCTACGC CAACCACCGC
    GGCCGCAGCC CGCTGGACCT GGCCGCCGAG GGCCGTGTAC TCAAGGCCCT GCAGGGCTGT
    GCGCAGCGCT TCCGGGAGAG GCACGCGGGC GGCAGCGGGG GTGCGGCCCC GGGCCCGAGG
    CTGGTGCTTG GCACCCCCAA CACCGTGACG AACCTGCACG TGGCTGCGCC GTCGGGGCCC
    GAGGCCGCCG AGTGCCTGGT GTGCTCGGAG CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC
    CAGCACCGCA CTGTGTGCGA GGAGTGCGCT CGCAGGATGA AGAAGTGCAT CAGATGCCAG
    GTGGTCATCG GCAAGAAGCT GCGACCAGAC GGCACAGAGG TGGCCAGCGC GACCCCCGCG
    TCCGGCCCGC CGCGGCAGCT GGTGGAGGAG CTGCAGAGTC GCTACCGGCA GATGGAGGAG
    CGCATCACCT GCCCCATCTG CATCGACAGC CACATCCGCC TCGTGTTCCA GTGCGGCCAC
    GGCGCGTGCG CACCCTGCGG CGCCGCGCTC AGCGCCTGCC CCATCTGCCG CCAGCCCATC
    CGCGACCGCA TCCAGATCTT CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274614.1
    CDS511..3357
    Translation

    Target ORF information:

    RefSeq Version XM_022418906.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418906.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGCACAAT GCCTTCTGGG TGGGCGATGT GGTACGGGTC
    ATCGATGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACAGATGAC
    ATGGCCCCCG CCCTGGGCCG CATTGGGAAG GTGGTGAAAG TTTTCAGAGA CGGTAACCTG
    CGTGTGGCAG TCGGTGGTCA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAGG ATGCCAACCT GGATGTGGCC GAACGTGCCA GGGAGAACAA AAGTGGGTGT
    GCCCACCTTG GGTGGCCGGC AGGAGGCAGG GCTGCCTTTG GCCTCCACTT AACTGAAGCC
    CTGCCCCACC CAGGCTTCCT GAGTGTTGCT CTGGAGAAGC TTCGAGCCCA GAAGAGTGAC
    CTGGAGCACC CAGGGAGACT GGTGGTGGAG GTGGCCTTGG GCAGTATGGC TGGGGCTCTG
    GACCAGCTGA GGCGGCACCC AGAGCAGGTG GACACCAAGA ACCAGGGCAG GACCGCTCTG
    CAGGTGGCCG CCTACCTAGG CCAGGTGGAG CTGGTGCGGC TGCTGCTGCA GGCACGAGCG
    GGCATGGACC TGGCAGATGA TGAGGGCAAT ACGGCGCTGC ACTATGCAGC CTTGGGGAAC
    CAGCCTGAGG CTGCCCGGTT GCTCCTGAGC TCCGGGTGTG GGGCCAATGC TCTTAACAGC
    ACCCGGAGCG CAGCACTGCA TGTGGCTGTG CAGAGGGGCT TCCTGGAGGT GGTGAAGGTC
    CTCTGTGAAC GTGGCTGTGA CGTCAACCTG CCTGATGCAC ACGCGGACAC ACCTCTGCAC
    TGTGCCATCT CGGCGGGCGC CGGCGCCAGC GGCATCGTGG AGGTCCTCAC TGAGGTGCCA
    GGTGTTGATG TCACGGCCAC TAACAGCCAG GGCTTCACCC TGCTGCATCA CGCATCCCTC
    AAGGGCCACA CGCTAGCTGT CAGGAGGATT CTAGCTCGGG CACGACAGCT GGTGGACGCC
    AAGAAGGAGG ATGGCTTCAC AGCCTTACAC CTGGCTGCCC TCAACAACCA CCGGGAAGTG
    GCCCAGATTC TGATCCGAGA GGGCCGCTGT GATGTGAACG TTCGCAACCG TAAGCTCCAG
    TCCCCCCTGC ACCTGGCCGT GCAGCAGGCT CATGTGGGGC TGGTGCCGCT GCTAGTGGAC
    GCGGGCTGCA GTGTCAACGC CGAGGATGAG GAAGGGGACA CAGCCTTGCA CGTGGCCCTG
    CAGCGTCATC AGCTGCTGCC CCTGGCAGCA GATGGGGCCG GGGGGGACCC AGGGCCCTTG
    CAGCTGCTGT CCAGGCTCCA GGCCTCAGGC CTCCCGGGCA GCGGGGAGCT GACGGTGGGA
    GCGGCGGTCG CATGCTTCCT GGCGCTGGAG GGCGCCGACG TGAGCTACGC CAACCACCGC
    GGCCGCAGCC CGCTGGACCT GGCCGCCGAG GGCCGTGTAC TCAAGGCCCT GCAGGGCTGT
    GCGCAGCGCT TCCGGGAGAG GCACGCGGGC GGCAGCGGGG GTGCGGCCCC GGGCCCGAGG
    CTGGTGCTTG GCACCCCCAA CACCGTGACG AACCTGCACG TGGCTGCGCC GTCGGGGCCC
    GAGGCCGCCG AGTGCCTGGT GTGCTCGGAG CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC
    CAGCACCGCA CTGTGTGCGA GGAGTGCGCT CGCAGGATGA AGAAGTGCAT CAGATGCCAG
    GTGGTCATCG GCAAGAAGCT GCGACCAGAC GGCACAGAGG TGGCCAGCGC GACCCCCGCG
    TCCGGCCCGC CGCGGCAGCT GGTGGAGGAG CTGCAGAGTC GCTACCGGCA GATGGAGGAG
    CGCATCACCT GCCCCATCTG CATCGACAGC CACATCCGCC TCGTGTTCCA GTGCGGCCAC
    GGCGCGTGCG CACCCTGCGG CGCCGCGCTC AGCGCCTGCC CCATCTGCCG CCAGCCCATC
    CGCGACCGCA TCCAGATCTT CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06737
    Clone ID Related Accession (Same CDS sequence) XM_022418906.1 , XM_014113817.1
    Accession Version XM_014113817.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2847bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGCACAAT GCCTTCTGGG TGGGCGATGT GGTACGGGTC
    ATCGATGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACAGATGAC
    ATGGCCCCCG CCCTGGGCCG CATTGGGAAG GTGGTGAAAG TTTTCAGAGA CGGTAACCTG
    CGTGTGGCAG TCGGTGGTCA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAGG ATGCCAACCT GGATGTGGCC GAACGTGCCA GGGAGAACAA AAGTGGGTGT
    GCCCACCTTG GGTGGCCGGC AGGAGGCAGG GCTGCCTTTG GCCTCCACTT AACTGAAGCC
    CTGCCCCACC CAGGCTTCCT GAGTGTTGCT CTGGAGAAGC TTCGAGCCCA GAAGAGTGAC
    CTGGAGCACC CAGGGAGACT GGTGGTGGAG GTGGCCTTGG GCAGTATGGC TGGGGCTCTG
    GACCAGCTGA GGCGGCACCC AGAGCAGGTG GACACCAAGA ACCAGGGCAG GACCGCTCTG
    CAGGTGGCCG CCTACCTAGG CCAGGTGGAG CTGGTGCGGC TGCTGCTGCA GGCACGAGCG
    GGCATGGACC TGGCAGATGA TGAGGGCAAT ACGGCGCTGC ACTATGCAGC CTTGGGGAAC
    CAGCCTGAGG CTGCCCGGTT GCTCCTGAGC TCCGGGTGTG GGGCCAATGC TCTTAACAGC
    ACCCGGAGCG CAGCACTGCA TGTGGCTGTG CAGAGGGGCT TCCTGGAGGT GGTGAAGGTC
    CTCTGTGAAC GTGGCTGTGA CGTCAACCTG CCTGATGCAC ACGCGGACAC ACCTCTGCAC
    TGTGCCATCT CGGCGGGCGC CGGCGCCAGC GGCATCGTGG AGGTCCTCAC TGAGGTGCCA
    GGTGTTGATG TCACGGCCAC TAACAGCCAG GGCTTCACCC TGCTGCATCA CGCATCCCTC
    AAGGGCCACA CGCTAGCTGT CAGGAGGATT CTAGCTCGGG CACGACAGCT GGTGGACGCC
    AAGAAGGAGG ATGGCTTCAC AGCCTTACAC CTGGCTGCCC TCAACAACCA CCGGGAAGTG
    GCCCAGATTC TGATCCGAGA GGGCCGCTGT GATGTGAACG TTCGCAACCG TAAGCTCCAG
    TCCCCCCTGC ACCTGGCCGT GCAGCAGGCT CATGTGGGGC TGGTGCCGCT GCTAGTGGAC
    GCGGGCTGCA GTGTCAACGC CGAGGATGAG GAAGGGGACA CAGCCTTGCA CGTGGCCCTG
    CAGCGTCATC AGCTGCTGCC CCTGGCAGCA GATGGGGCCG GGGGGGACCC AGGGCCCTTG
    CAGCTGCTGT CCAGGCTCCA GGCCTCAGGC CTCCCGGGCA GCGGGGAGCT GACGGTGGGA
    GCGGCGGTCG CATGCTTCCT GGCGCTGGAG GGCGCCGACG TGAGCTACGC CAACCACCGC
    GGCCGCAGCC CGCTGGACCT GGCCGCCGAG GGCCGTGTAC TCAAGGCCCT GCAGGGCTGT
    GCGCAGCGCT TCCGGGAGAG GCACGCGGGC GGCAGCGGGG GTGCGGCCCC GGGCCCGAGG
    CTGGTGCTTG GCACCCCCAA CACCGTGACG AACCTGCACG TGGCTGCGCC GTCGGGGCCC
    GAGGCCGCCG AGTGCCTGGT GTGCTCGGAG CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC
    CAGCACCGCA CTGTGTGCGA GGAGTGCGCT CGCAGGATGA AGAAGTGCAT CAGATGCCAG
    GTGGTCATCG GCAAGAAGCT GCGACCAGAC GGCACAGAGG TGGCCAGCGC GACCCCCGCG
    TCCGGCCCGC CGCGGCAGCT GGTGGAGGAG CTGCAGAGTC GCTACCGGCA GATGGAGGAG
    CGCATCACCT GCCCCATCTG CATCGACAGC CACATCCGCC TCGTGTTCCA GTGCGGCCAC
    GGCGCGTGCG CACCCTGCGG CGCCGCGCTC AGCGCCTGCC CCATCTGCCG CCAGCCCATC
    CGCGACCGCA TCCAGATCTT CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969292.1
    CDS420..3266
    Translation

    Target ORF information:

    RefSeq Version XM_014113817.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113817.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGCACAAT GCCTTCTGGG TGGGCGATGT GGTACGGGTC
    ATCGATGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACAGATGAC
    ATGGCCCCCG CCCTGGGCCG CATTGGGAAG GTGGTGAAAG TTTTCAGAGA CGGTAACCTG
    CGTGTGGCAG TCGGTGGTCA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAGG ATGCCAACCT GGATGTGGCC GAACGTGCCA GGGAGAACAA AAGTGGGTGT
    GCCCACCTTG GGTGGCCGGC AGGAGGCAGG GCTGCCTTTG GCCTCCACTT AACTGAAGCC
    CTGCCCCACC CAGGCTTCCT GAGTGTTGCT CTGGAGAAGC TTCGAGCCCA GAAGAGTGAC
    CTGGAGCACC CAGGGAGACT GGTGGTGGAG GTGGCCTTGG GCAGTATGGC TGGGGCTCTG
    GACCAGCTGA GGCGGCACCC AGAGCAGGTG GACACCAAGA ACCAGGGCAG GACCGCTCTG
    CAGGTGGCCG CCTACCTAGG CCAGGTGGAG CTGGTGCGGC TGCTGCTGCA GGCACGAGCG
    GGCATGGACC TGGCAGATGA TGAGGGCAAT ACGGCGCTGC ACTATGCAGC CTTGGGGAAC
    CAGCCTGAGG CTGCCCGGTT GCTCCTGAGC TCCGGGTGTG GGGCCAATGC TCTTAACAGC
    ACCCGGAGCG CAGCACTGCA TGTGGCTGTG CAGAGGGGCT TCCTGGAGGT GGTGAAGGTC
    CTCTGTGAAC GTGGCTGTGA CGTCAACCTG CCTGATGCAC ACGCGGACAC ACCTCTGCAC
    TGTGCCATCT CGGCGGGCGC CGGCGCCAGC GGCATCGTGG AGGTCCTCAC TGAGGTGCCA
    GGTGTTGATG TCACGGCCAC TAACAGCCAG GGCTTCACCC TGCTGCATCA CGCATCCCTC
    AAGGGCCACA CGCTAGCTGT CAGGAGGATT CTAGCTCGGG CACGACAGCT GGTGGACGCC
    AAGAAGGAGG ATGGCTTCAC AGCCTTACAC CTGGCTGCCC TCAACAACCA CCGGGAAGTG
    GCCCAGATTC TGATCCGAGA GGGCCGCTGT GATGTGAACG TTCGCAACCG TAAGCTCCAG
    TCCCCCCTGC ACCTGGCCGT GCAGCAGGCT CATGTGGGGC TGGTGCCGCT GCTAGTGGAC
    GCGGGCTGCA GTGTCAACGC CGAGGATGAG GAAGGGGACA CAGCCTTGCA CGTGGCCCTG
    CAGCGTCATC AGCTGCTGCC CCTGGCAGCA GATGGGGCCG GGGGGGACCC AGGGCCCTTG
    CAGCTGCTGT CCAGGCTCCA GGCCTCAGGC CTCCCGGGCA GCGGGGAGCT GACGGTGGGA
    GCGGCGGTCG CATGCTTCCT GGCGCTGGAG GGCGCCGACG TGAGCTACGC CAACCACCGC
    GGCCGCAGCC CGCTGGACCT GGCCGCCGAG GGCCGTGTAC TCAAGGCCCT GCAGGGCTGT
    GCGCAGCGCT TCCGGGAGAG GCACGCGGGC GGCAGCGGGG GTGCGGCCCC GGGCCCGAGG
    CTGGTGCTTG GCACCCCCAA CACCGTGACG AACCTGCACG TGGCTGCGCC GTCGGGGCCC
    GAGGCCGCCG AGTGCCTGGT GTGCTCGGAG CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC
    CAGCACCGCA CTGTGTGCGA GGAGTGCGCT CGCAGGATGA AGAAGTGCAT CAGATGCCAG
    GTGGTCATCG GCAAGAAGCT GCGACCAGAC GGCACAGAGG TGGCCAGCGC GACCCCCGCG
    TCCGGCCCGC CGCGGCAGCT GGTGGAGGAG CTGCAGAGTC GCTACCGGCA GATGGAGGAG
    CGCATCACCT GCCCCATCTG CATCGACAGC CACATCCGCC TCGTGTTCCA GTGCGGCCAC
    GGCGCGTGCG CACCCTGCGG CGCCGCGCTC AGCGCCTGCC CCATCTGCCG CCAGCCCATC
    CGCGACCGCA TCCAGATCTT CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06738
    Clone ID Related Accession (Same CDS sequence) XM_022418907.1 , XM_014113818.1
    Accession Version XM_022418907.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGCACAAT GCCTTCTGGG TGGGCGATGT GGTACGGGTC
    ATCGATGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACAGATGAC
    ATGGCCCCCG CCCTGGGCCG CATTGGGAAG GTGGTGAAAG TTTTCAGAGA CGGTAACCTG
    CGTGTGGCAG TCGGTGGTCA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAGG ATGCCAACCT GGATGTGGCC GAACGTGCCA GGGAGAACAA AAGCTTCCTG
    AGTGTTGCTC TGGAGAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAGACTG
    GTGGTGGAGG TGGCCTTGGG CAGTATGGCT GGGGCTCTGG ACCAGCTGAG GCGGCACCCA
    GAGCAGGTGG ACACCAAGAA CCAGGGCAGG ACCGCTCTGC AGGTGGCCGC CTACCTAGGC
    CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCACGAGCGG GCATGGACCT GGCAGATGAT
    GAGGGCAATA CGGCGCTGCA CTATGCAGCC TTGGGGAACC AGCCTGAGGC TGCCCGGTTG
    CTCCTGAGCT CCGGGTGTGG GGCCAATGCT CTTAACAGCA CCCGGAGCGC AGCACTGCAT
    GTGGCTGTGC AGAGGGGCTT CCTGGAGGTG GTGAAGGTCC TCTGTGAACG TGGCTGTGAC
    GTCAACCTGC CTGATGCACA CGCGGACACA CCTCTGCACT GTGCCATCTC GGCGGGCGCC
    GGCGCCAGCG GCATCGTGGA GGTCCTCACT GAGGTGCCAG GTGTTGATGT CACGGCCACT
    AACAGCCAGG GCTTCACCCT GCTGCATCAC GCATCCCTCA AGGGCCACAC GCTAGCTGTC
    AGGAGGATTC TAGCTCGGGC ACGACAGCTG GTGGACGCCA AGAAGGAGGA TGGCTTCACA
    GCCTTACACC TGGCTGCCCT CAACAACCAC CGGGAAGTGG CCCAGATTCT GATCCGAGAG
    GGCCGCTGTG ATGTGAACGT TCGCAACCGT AAGCTCCAGT CCCCCCTGCA CCTGGCCGTG
    CAGCAGGCTC ATGTGGGGCT GGTGCCGCTG CTAGTGGACG CGGGCTGCAG TGTCAACGCC
    GAGGATGAGG AAGGGGACAC AGCCTTGCAC GTGGCCCTGC AGCGTCATCA GCTGCTGCCC
    CTGGCAGCAG ATGGGGCCGG GGGGGACCCA GGGCCCTTGC AGCTGCTGTC CAGGCTCCAG
    GCCTCAGGCC TCCCGGGCAG CGGGGAGCTG ACGGTGGGAG CGGCGGTCGC ATGCTTCCTG
    GCGCTGGAGG GCGCCGACGT GAGCTACGCC AACCACCGCG GCCGCAGCCC GCTGGACCTG
    GCCGCCGAGG GCCGTGTACT CAAGGCCCTG CAGGGCTGTG CGCAGCGCTT CCGGGAGAGG
    CACGCGGGCG GCAGCGGGGG TGCGGCCCCG GGCCCGAGGC TGGTGCTTGG CACCCCCAAC
    ACCGTGACGA ACCTGCACGT GGCTGCGCCG TCGGGGCCCG AGGCCGCCGA GTGCCTGGTG
    TGCTCGGAGC TGGCGCTGCT GGTGCTGTTC TCGCCGTGCC AGCACCGCAC TGTGTGCGAG
    GAGTGCGCTC GCAGGATGAA GAAGTGCATC AGATGCCAGG TGGTCATCGG CAAGAAGCTG
    CGACCAGACG GCACAGAGGT GGCCAGCGCG ACCCCCGCGT CCGGCCCGCC GCGGCAGCTG
    GTGGAGGAGC TGCAGAGTCG CTACCGGCAG ATGGAGGAGC GCATCACCTG CCCCATCTGC
    ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCACG GCGCGTGCGC ACCCTGCGGC
    GCCGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT CCAGATCTTC
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274615.1
    CDS510..3275
    Translation

    Target ORF information:

    RefSeq Version XM_022418907.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418907.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGCACAAT GCCTTCTGGG TGGGCGATGT GGTACGGGTC
    ATCGATGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACAGATGAC
    ATGGCCCCCG CCCTGGGCCG CATTGGGAAG GTGGTGAAAG TTTTCAGAGA CGGTAACCTG
    CGTGTGGCAG TCGGTGGTCA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAGG ATGCCAACCT GGATGTGGCC GAACGTGCCA GGGAGAACAA AAGCTTCCTG
    AGTGTTGCTC TGGAGAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAGACTG
    GTGGTGGAGG TGGCCTTGGG CAGTATGGCT GGGGCTCTGG ACCAGCTGAG GCGGCACCCA
    GAGCAGGTGG ACACCAAGAA CCAGGGCAGG ACCGCTCTGC AGGTGGCCGC CTACCTAGGC
    CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCACGAGCGG GCATGGACCT GGCAGATGAT
    GAGGGCAATA CGGCGCTGCA CTATGCAGCC TTGGGGAACC AGCCTGAGGC TGCCCGGTTG
    CTCCTGAGCT CCGGGTGTGG GGCCAATGCT CTTAACAGCA CCCGGAGCGC AGCACTGCAT
    GTGGCTGTGC AGAGGGGCTT CCTGGAGGTG GTGAAGGTCC TCTGTGAACG TGGCTGTGAC
    GTCAACCTGC CTGATGCACA CGCGGACACA CCTCTGCACT GTGCCATCTC GGCGGGCGCC
    GGCGCCAGCG GCATCGTGGA GGTCCTCACT GAGGTGCCAG GTGTTGATGT CACGGCCACT
    AACAGCCAGG GCTTCACCCT GCTGCATCAC GCATCCCTCA AGGGCCACAC GCTAGCTGTC
    AGGAGGATTC TAGCTCGGGC ACGACAGCTG GTGGACGCCA AGAAGGAGGA TGGCTTCACA
    GCCTTACACC TGGCTGCCCT CAACAACCAC CGGGAAGTGG CCCAGATTCT GATCCGAGAG
    GGCCGCTGTG ATGTGAACGT TCGCAACCGT AAGCTCCAGT CCCCCCTGCA CCTGGCCGTG
    CAGCAGGCTC ATGTGGGGCT GGTGCCGCTG CTAGTGGACG CGGGCTGCAG TGTCAACGCC
    GAGGATGAGG AAGGGGACAC AGCCTTGCAC GTGGCCCTGC AGCGTCATCA GCTGCTGCCC
    CTGGCAGCAG ATGGGGCCGG GGGGGACCCA GGGCCCTTGC AGCTGCTGTC CAGGCTCCAG
    GCCTCAGGCC TCCCGGGCAG CGGGGAGCTG ACGGTGGGAG CGGCGGTCGC ATGCTTCCTG
    GCGCTGGAGG GCGCCGACGT GAGCTACGCC AACCACCGCG GCCGCAGCCC GCTGGACCTG
    GCCGCCGAGG GCCGTGTACT CAAGGCCCTG CAGGGCTGTG CGCAGCGCTT CCGGGAGAGG
    CACGCGGGCG GCAGCGGGGG TGCGGCCCCG GGCCCGAGGC TGGTGCTTGG CACCCCCAAC
    ACCGTGACGA ACCTGCACGT GGCTGCGCCG TCGGGGCCCG AGGCCGCCGA GTGCCTGGTG
    TGCTCGGAGC TGGCGCTGCT GGTGCTGTTC TCGCCGTGCC AGCACCGCAC TGTGTGCGAG
    GAGTGCGCTC GCAGGATGAA GAAGTGCATC AGATGCCAGG TGGTCATCGG CAAGAAGCTG
    CGACCAGACG GCACAGAGGT GGCCAGCGCG ACCCCCGCGT CCGGCCCGCC GCGGCAGCTG
    GTGGAGGAGC TGCAGAGTCG CTACCGGCAG ATGGAGGAGC GCATCACCTG CCCCATCTGC
    ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCACG GCGCGTGCGC ACCCTGCGGC
    GCCGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT CCAGATCTTC
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06738
    Clone ID Related Accession (Same CDS sequence) XM_022418907.1 , XM_014113818.1
    Accession Version XM_014113818.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2766bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGCACAAT GCCTTCTGGG TGGGCGATGT GGTACGGGTC
    ATCGATGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACAGATGAC
    ATGGCCCCCG CCCTGGGCCG CATTGGGAAG GTGGTGAAAG TTTTCAGAGA CGGTAACCTG
    CGTGTGGCAG TCGGTGGTCA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAGG ATGCCAACCT GGATGTGGCC GAACGTGCCA GGGAGAACAA AAGCTTCCTG
    AGTGTTGCTC TGGAGAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAGACTG
    GTGGTGGAGG TGGCCTTGGG CAGTATGGCT GGGGCTCTGG ACCAGCTGAG GCGGCACCCA
    GAGCAGGTGG ACACCAAGAA CCAGGGCAGG ACCGCTCTGC AGGTGGCCGC CTACCTAGGC
    CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCACGAGCGG GCATGGACCT GGCAGATGAT
    GAGGGCAATA CGGCGCTGCA CTATGCAGCC TTGGGGAACC AGCCTGAGGC TGCCCGGTTG
    CTCCTGAGCT CCGGGTGTGG GGCCAATGCT CTTAACAGCA CCCGGAGCGC AGCACTGCAT
    GTGGCTGTGC AGAGGGGCTT CCTGGAGGTG GTGAAGGTCC TCTGTGAACG TGGCTGTGAC
    GTCAACCTGC CTGATGCACA CGCGGACACA CCTCTGCACT GTGCCATCTC GGCGGGCGCC
    GGCGCCAGCG GCATCGTGGA GGTCCTCACT GAGGTGCCAG GTGTTGATGT CACGGCCACT
    AACAGCCAGG GCTTCACCCT GCTGCATCAC GCATCCCTCA AGGGCCACAC GCTAGCTGTC
    AGGAGGATTC TAGCTCGGGC ACGACAGCTG GTGGACGCCA AGAAGGAGGA TGGCTTCACA
    GCCTTACACC TGGCTGCCCT CAACAACCAC CGGGAAGTGG CCCAGATTCT GATCCGAGAG
    GGCCGCTGTG ATGTGAACGT TCGCAACCGT AAGCTCCAGT CCCCCCTGCA CCTGGCCGTG
    CAGCAGGCTC ATGTGGGGCT GGTGCCGCTG CTAGTGGACG CGGGCTGCAG TGTCAACGCC
    GAGGATGAGG AAGGGGACAC AGCCTTGCAC GTGGCCCTGC AGCGTCATCA GCTGCTGCCC
    CTGGCAGCAG ATGGGGCCGG GGGGGACCCA GGGCCCTTGC AGCTGCTGTC CAGGCTCCAG
    GCCTCAGGCC TCCCGGGCAG CGGGGAGCTG ACGGTGGGAG CGGCGGTCGC ATGCTTCCTG
    GCGCTGGAGG GCGCCGACGT GAGCTACGCC AACCACCGCG GCCGCAGCCC GCTGGACCTG
    GCCGCCGAGG GCCGTGTACT CAAGGCCCTG CAGGGCTGTG CGCAGCGCTT CCGGGAGAGG
    CACGCGGGCG GCAGCGGGGG TGCGGCCCCG GGCCCGAGGC TGGTGCTTGG CACCCCCAAC
    ACCGTGACGA ACCTGCACGT GGCTGCGCCG TCGGGGCCCG AGGCCGCCGA GTGCCTGGTG
    TGCTCGGAGC TGGCGCTGCT GGTGCTGTTC TCGCCGTGCC AGCACCGCAC TGTGTGCGAG
    GAGTGCGCTC GCAGGATGAA GAAGTGCATC AGATGCCAGG TGGTCATCGG CAAGAAGCTG
    CGACCAGACG GCACAGAGGT GGCCAGCGCG ACCCCCGCGT CCGGCCCGCC GCGGCAGCTG
    GTGGAGGAGC TGCAGAGTCG CTACCGGCAG ATGGAGGAGC GCATCACCTG CCCCATCTGC
    ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCACG GCGCGTGCGC ACCCTGCGGC
    GCCGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT CCAGATCTTC
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969293.1
    CDS420..3185
    Translation

    Target ORF information:

    RefSeq Version XM_014113818.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113818.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGACCCAG ACCCCCAGGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA TGGCGGTGAG GGCGGCGTGG GCACAGTGGT GGAGCTCGGC
    CGCCATGGTA GCCCCTCGAC CCCCGACCGC ACGGTAGTTG TGCAGTGGGA CCACGGCACC
    CGCACCAACT ACCGCGCTGG CTACCAGGGT GCCCACGACC TGCTGCTCTA TGACAACGCC
    CAGATCGGCG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGCCTGCGA
    GGCATGCGCT GGAAGTGCCG CGTCTGCTTC GACTACGACC TGTGCACGCA GTGCTACATG
    CACAACAAGC ATGACCTCAC CCACGCCTTC GAGCGCTACG AGACAGCCCA CTCCCGCCCG
    GTCACGCTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAT TGAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TGCGGGGCCC TGACTGGGAG TGGGGTTCGC AGGATGGAGG GGAAGGGAAG
    CTGGGCCGAG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CGGGCCGGAG TGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAATGTG TACCGTGTGG GTCACAAGGG CAAGGTGGAC
    CTCAAGTGTG TGAACGAGGC AGCTGGTGGC TTCTACTACA AGGAGCACCT CCCGAAGCTT
    GGTAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCCGACA GCCAGCCTTT CCAGCACGGG
    GACAAGGTTA AGTGTCTGCT GGACACAGAC ATCCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCAGGATGGC GGAGCACAAT GCCTTCTGGG TGGGCGATGT GGTACGGGTC
    ATCGATGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACAGATGAC
    ATGGCCCCCG CCCTGGGCCG CATTGGGAAG GTGGTGAAAG TTTTCAGAGA CGGTAACCTG
    CGTGTGGCAG TCGGTGGTCA GCTGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAGG ATGCCAACCT GGATGTGGCC GAACGTGCCA GGGAGAACAA AAGCTTCCTG
    AGTGTTGCTC TGGAGAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAGACTG
    GTGGTGGAGG TGGCCTTGGG CAGTATGGCT GGGGCTCTGG ACCAGCTGAG GCGGCACCCA
    GAGCAGGTGG ACACCAAGAA CCAGGGCAGG ACCGCTCTGC AGGTGGCCGC CTACCTAGGC
    CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCACGAGCGG GCATGGACCT GGCAGATGAT
    GAGGGCAATA CGGCGCTGCA CTATGCAGCC TTGGGGAACC AGCCTGAGGC TGCCCGGTTG
    CTCCTGAGCT CCGGGTGTGG GGCCAATGCT CTTAACAGCA CCCGGAGCGC AGCACTGCAT
    GTGGCTGTGC AGAGGGGCTT CCTGGAGGTG GTGAAGGTCC TCTGTGAACG TGGCTGTGAC
    GTCAACCTGC CTGATGCACA CGCGGACACA CCTCTGCACT GTGCCATCTC GGCGGGCGCC
    GGCGCCAGCG GCATCGTGGA GGTCCTCACT GAGGTGCCAG GTGTTGATGT CACGGCCACT
    AACAGCCAGG GCTTCACCCT GCTGCATCAC GCATCCCTCA AGGGCCACAC GCTAGCTGTC
    AGGAGGATTC TAGCTCGGGC ACGACAGCTG GTGGACGCCA AGAAGGAGGA TGGCTTCACA
    GCCTTACACC TGGCTGCCCT CAACAACCAC CGGGAAGTGG CCCAGATTCT GATCCGAGAG
    GGCCGCTGTG ATGTGAACGT TCGCAACCGT AAGCTCCAGT CCCCCCTGCA CCTGGCCGTG
    CAGCAGGCTC ATGTGGGGCT GGTGCCGCTG CTAGTGGACG CGGGCTGCAG TGTCAACGCC
    GAGGATGAGG AAGGGGACAC AGCCTTGCAC GTGGCCCTGC AGCGTCATCA GCTGCTGCCC
    CTGGCAGCAG ATGGGGCCGG GGGGGACCCA GGGCCCTTGC AGCTGCTGTC CAGGCTCCAG
    GCCTCAGGCC TCCCGGGCAG CGGGGAGCTG ACGGTGGGAG CGGCGGTCGC ATGCTTCCTG
    GCGCTGGAGG GCGCCGACGT GAGCTACGCC AACCACCGCG GCCGCAGCCC GCTGGACCTG
    GCCGCCGAGG GCCGTGTACT CAAGGCCCTG CAGGGCTGTG CGCAGCGCTT CCGGGAGAGG
    CACGCGGGCG GCAGCGGGGG TGCGGCCCCG GGCCCGAGGC TGGTGCTTGG CACCCCCAAC
    ACCGTGACGA ACCTGCACGT GGCTGCGCCG TCGGGGCCCG AGGCCGCCGA GTGCCTGGTG
    TGCTCGGAGC TGGCGCTGCT GGTGCTGTTC TCGCCGTGCC AGCACCGCAC TGTGTGCGAG
    GAGTGCGCTC GCAGGATGAA GAAGTGCATC AGATGCCAGG TGGTCATCGG CAAGAAGCTG
    CGACCAGACG GCACAGAGGT GGCCAGCGCG ACCCCCGCGT CCGGCCCGCC GCGGCAGCTG
    GTGGAGGAGC TGCAGAGTCG CTACCGGCAG ATGGAGGAGC GCATCACCTG CCCCATCTGC
    ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCACG GCGCGTGCGC ACCCTGCGGC
    GCCGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT CCAGATCTTC
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06739
    Clone ID Related Accession (Same CDS sequence) XM_005620393.2 , XM_022418908.1
    Accession Version XM_005620393.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005620393.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCGCTGGA AGTGCCGCGT CTGCTTCGAC TACGACCTGT GCACGCAGTG CTACATGCAC 
    AACAAGCATG ACCTCACCCA CGCCTTCGAG CGCTACGAGA CAGCCCACTC CCGCCCGGTC
    ACGCTGAGTC CCCGCCAGGG CCTCCCGAGG ATCCCATTGA GGGGCATCTT CCAGGGGGCG
    AAGGTGGTGC GGGGCCCTGA CTGGGAGTGG GGTTCGCAGG ATGGAGGGGA AGGGAAGCTG
    GGCCGAGTGG TGGACATCCG TGGCTGGGAT GTGGAGACGG GCCGGAGTGT GGCCAGTGTG
    ACGTGGGCTG ATGGCACCAC CAATGTGTAC CGTGTGGGTC ACAAGGGCAA GGTGGACCTC
    AAGTGTGTGA ACGAGGCAGC TGGTGGCTTC TACTACAAGG AGCACCTCCC GAAGCTTGGT
    AAGCCAGCAG AGCTGCAGCG CAGGGTGAGT GCCGACAGCC AGCCTTTCCA GCACGGGGAC
    AAGGTTAAGT GTCTGCTGGA CACAGACATC CTGAGGGAGA TGCAGGAAGG CCACGGCGGG
    TGGAACCCCA GGATGGCGGA GTTTATCGGA CAGACGGGCA CCGTGCACCG CATCACGGAC
    CGTGGGGATG TGCGTGTGCA GTTCGGCCGT GAGACCCGCT GGACCTTCCA CCCTGGGGCT
    CTCACCAAGC ACAATGCCTT CTGGGTGGGC GATGTGGTAC GGGTCATCGA TGACCTTGAC
    ACAGTGAAGC GGCTGCAGGC TGGGCATGGC GAGTGGACAG ATGACATGGC CCCCGCCCTG
    GGCCGCATTG GGAAGGTGGT GAAAGTTTTC AGAGACGGTA ACCTGCGTGT GGCAGTCGGT
    GGTCAGCTGT GGACCTTCAG CCCCTCCTGC CTGGTGGCCT ACCGGCCTGA GGAGGATGCC
    AACCTGGATG TGGCCGAACG TGCCAGGGAG AACAAAAGCT TCCTGAGTGT TGCTCTGGAG
    AAGCTTCGAG CCCAGAAGAG TGACCTGGAG CACCCAGGGA GACTGGTGGT GGAGGTGGCC
    TTGGGCAGTA TGGCTGGGGC TCTGGACCAG CTGAGGCGGC ACCCAGAGCA GGTGGACACC
    AAGAACCAGG GCAGGACCGC TCTGCAGGTG GCCGCCTACC TAGGCCAGGT GGAGCTGGTG
    CGGCTGCTGC TGCAGGCACG AGCGGGCATG GACCTGGCAG ATGATGAGGG CAATACGGCG
    CTGCACTATG CAGCCTTGGG GAACCAGCCT GAGGCTGCCC GGTTGCTCCT GAGCTCCGGG
    TGTGGGGCCA ATGCTCTTAA CAGCACCCGG AGCGCAGCAC TGCATGTGGC TGTGCAGAGG
    GGCTTCCTGG AGGTGGTGAA GGTCCTCTGT GAACGTGGCT GTGACGTCAA CCTGCCTGAT
    GCACACGCGG ACACACCTCT GCACTGTGCC ATCTCGGCGG GCGCCGGCGC CAGCGGCATC
    GTGGAGGTCC TCACTGAGGT GCCAGGTGTT GATGTCACGG CCACTAACAG CCAGGGCTTC
    ACCCTGCTGC ATCACGCATC CCTCAAGGGC CACACGCTAG CTGTCAGGAG GATTCTAGCT
    CGGGCACGAC AGCTGGTGGA CGCCAAGAAG GAGGATGGCT TCACAGCCTT ACACCTGGCT
    GCCCTCAACA ACCACCGGGA AGTGGCCCAG ATTCTGATCC GAGAGGGCCG CTGTGATGTG
    AACGTTCGCA ACCGTAAGCT CCAGTCCCCC CTGCACCTGG CCGTGCAGCA GGCTCATGTG
    GGGCTGGTGC CGCTGCTAGT GGACGCGGGC TGCAGTGTCA ACGCCGAGGA TGAGGAAGGG
    GACACAGCCT TGCACGTGGC CCTGCAGCGT CATCAGCTGC TGCCCCTGGC AGCAGATGGG
    GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG CTGTCCAGGC TCCAGGCCTC AGGCCTCCCG
    GGCAGCGGGG AGCTGACGGT GGGAGCGGCG GTCGCATGCT TCCTGGCGCT GGAGGGCGCC
    GACGTGAGCT ACGCCAACCA CCGCGGCCGC AGCCCGCTGG ACCTGGCCGC CGAGGGCCGT
    GTACTCAAGG CCCTGCAGGG CTGTGCGCAG CGCTTCCGGG AGAGGCACGC GGGCGGCAGC
    GGGGGTGCGG CCCCGGGCCC GAGGCTGGTG CTTGGCACCC CCAACACCGT GACGAACCTG
    CACGTGGCTG CGCCGTCGGG GCCCGAGGCC GCCGAGTGCC TGGTGTGCTC GGAGCTGGCG
    CTGCTGGTGC TGTTCTCGCC GTGCCAGCAC CGCACTGTGT GCGAGGAGTG CGCTCGCAGG
    ATGAAGAAGT GCATCAGATG CCAGGTGGTC ATCGGCAAGA AGCTGCGACC AGACGGCACA
    GAGGTGGCCA GCGCGACCCC CGCGTCCGGC CCGCCGCGGC AGCTGGTGGA GGAGCTGCAG
    AGTCGCTACC GGCAGATGGA GGAGCGCATC ACCTGCCCCA TCTGCATCGA CAGCCACATC
    CGCCTCGTGT TCCAGTGCGG CCACGGCGCG TGCGCACCCT GCGGCGCCGC GCTCAGCGCC
    TGCCCCATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA TCTTCGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005620450.1
    CDS506..3076
    Translation

    Target ORF information:

    RefSeq Version XM_005620393.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005620393.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCGCTGGA AGTGCCGCGT CTGCTTCGAC TACGACCTGT GCACGCAGTG CTACATGCAC 
    AACAAGCATG ACCTCACCCA CGCCTTCGAG CGCTACGAGA CAGCCCACTC CCGCCCGGTC
    ACGCTGAGTC CCCGCCAGGG CCTCCCGAGG ATCCCATTGA GGGGCATCTT CCAGGGGGCG
    AAGGTGGTGC GGGGCCCTGA CTGGGAGTGG GGTTCGCAGG ATGGAGGGGA AGGGAAGCTG
    GGCCGAGTGG TGGACATCCG TGGCTGGGAT GTGGAGACGG GCCGGAGTGT GGCCAGTGTG
    ACGTGGGCTG ATGGCACCAC CAATGTGTAC CGTGTGGGTC ACAAGGGCAA GGTGGACCTC
    AAGTGTGTGA ACGAGGCAGC TGGTGGCTTC TACTACAAGG AGCACCTCCC GAAGCTTGGT
    AAGCCAGCAG AGCTGCAGCG CAGGGTGAGT GCCGACAGCC AGCCTTTCCA GCACGGGGAC
    AAGGTTAAGT GTCTGCTGGA CACAGACATC CTGAGGGAGA TGCAGGAAGG CCACGGCGGG
    TGGAACCCCA GGATGGCGGA GTTTATCGGA CAGACGGGCA CCGTGCACCG CATCACGGAC
    CGTGGGGATG TGCGTGTGCA GTTCGGCCGT GAGACCCGCT GGACCTTCCA CCCTGGGGCT
    CTCACCAAGC ACAATGCCTT CTGGGTGGGC GATGTGGTAC GGGTCATCGA TGACCTTGAC
    ACAGTGAAGC GGCTGCAGGC TGGGCATGGC GAGTGGACAG ATGACATGGC CCCCGCCCTG
    GGCCGCATTG GGAAGGTGGT GAAAGTTTTC AGAGACGGTA ACCTGCGTGT GGCAGTCGGT
    GGTCAGCTGT GGACCTTCAG CCCCTCCTGC CTGGTGGCCT ACCGGCCTGA GGAGGATGCC
    AACCTGGATG TGGCCGAACG TGCCAGGGAG AACAAAAGCT TCCTGAGTGT TGCTCTGGAG
    AAGCTTCGAG CCCAGAAGAG TGACCTGGAG CACCCAGGGA GACTGGTGGT GGAGGTGGCC
    TTGGGCAGTA TGGCTGGGGC TCTGGACCAG CTGAGGCGGC ACCCAGAGCA GGTGGACACC
    AAGAACCAGG GCAGGACCGC TCTGCAGGTG GCCGCCTACC TAGGCCAGGT GGAGCTGGTG
    CGGCTGCTGC TGCAGGCACG AGCGGGCATG GACCTGGCAG ATGATGAGGG CAATACGGCG
    CTGCACTATG CAGCCTTGGG GAACCAGCCT GAGGCTGCCC GGTTGCTCCT GAGCTCCGGG
    TGTGGGGCCA ATGCTCTTAA CAGCACCCGG AGCGCAGCAC TGCATGTGGC TGTGCAGAGG
    GGCTTCCTGG AGGTGGTGAA GGTCCTCTGT GAACGTGGCT GTGACGTCAA CCTGCCTGAT
    GCACACGCGG ACACACCTCT GCACTGTGCC ATCTCGGCGG GCGCCGGCGC CAGCGGCATC
    GTGGAGGTCC TCACTGAGGT GCCAGGTGTT GATGTCACGG CCACTAACAG CCAGGGCTTC
    ACCCTGCTGC ATCACGCATC CCTCAAGGGC CACACGCTAG CTGTCAGGAG GATTCTAGCT
    CGGGCACGAC AGCTGGTGGA CGCCAAGAAG GAGGATGGCT TCACAGCCTT ACACCTGGCT
    GCCCTCAACA ACCACCGGGA AGTGGCCCAG ATTCTGATCC GAGAGGGCCG CTGTGATGTG
    AACGTTCGCA ACCGTAAGCT CCAGTCCCCC CTGCACCTGG CCGTGCAGCA GGCTCATGTG
    GGGCTGGTGC CGCTGCTAGT GGACGCGGGC TGCAGTGTCA ACGCCGAGGA TGAGGAAGGG
    GACACAGCCT TGCACGTGGC CCTGCAGCGT CATCAGCTGC TGCCCCTGGC AGCAGATGGG
    GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG CTGTCCAGGC TCCAGGCCTC AGGCCTCCCG
    GGCAGCGGGG AGCTGACGGT GGGAGCGGCG GTCGCATGCT TCCTGGCGCT GGAGGGCGCC
    GACGTGAGCT ACGCCAACCA CCGCGGCCGC AGCCCGCTGG ACCTGGCCGC CGAGGGCCGT
    GTACTCAAGG CCCTGCAGGG CTGTGCGCAG CGCTTCCGGG AGAGGCACGC GGGCGGCAGC
    GGGGGTGCGG CCCCGGGCCC GAGGCTGGTG CTTGGCACCC CCAACACCGT GACGAACCTG
    CACGTGGCTG CGCCGTCGGG GCCCGAGGCC GCCGAGTGCC TGGTGTGCTC GGAGCTGGCG
    CTGCTGGTGC TGTTCTCGCC GTGCCAGCAC CGCACTGTGT GCGAGGAGTG CGCTCGCAGG
    ATGAAGAAGT GCATCAGATG CCAGGTGGTC ATCGGCAAGA AGCTGCGACC AGACGGCACA
    GAGGTGGCCA GCGCGACCCC CGCGTCCGGC CCGCCGCGGC AGCTGGTGGA GGAGCTGCAG
    AGTCGCTACC GGCAGATGGA GGAGCGCATC ACCTGCCCCA TCTGCATCGA CAGCCACATC
    CGCCTCGTGT TCCAGTGCGG CCACGGCGCG TGCGCACCCT GCGGCGCCGC GCTCAGCGCC
    TGCCCCATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA TCTTCGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe06739
    Clone ID Related Accession (Same CDS sequence) XM_005620393.2 , XM_022418908.1
    Accession Version XM_022418908.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2571bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCGCTGGA AGTGCCGCGT CTGCTTCGAC TACGACCTGT GCACGCAGTG CTACATGCAC 
    AACAAGCATG ACCTCACCCA CGCCTTCGAG CGCTACGAGA CAGCCCACTC CCGCCCGGTC
    ACGCTGAGTC CCCGCCAGGG CCTCCCGAGG ATCCCATTGA GGGGCATCTT CCAGGGGGCG
    AAGGTGGTGC GGGGCCCTGA CTGGGAGTGG GGTTCGCAGG ATGGAGGGGA AGGGAAGCTG
    GGCCGAGTGG TGGACATCCG TGGCTGGGAT GTGGAGACGG GCCGGAGTGT GGCCAGTGTG
    ACGTGGGCTG ATGGCACCAC CAATGTGTAC CGTGTGGGTC ACAAGGGCAA GGTGGACCTC
    AAGTGTGTGA ACGAGGCAGC TGGTGGCTTC TACTACAAGG AGCACCTCCC GAAGCTTGGT
    AAGCCAGCAG AGCTGCAGCG CAGGGTGAGT GCCGACAGCC AGCCTTTCCA GCACGGGGAC
    AAGGTTAAGT GTCTGCTGGA CACAGACATC CTGAGGGAGA TGCAGGAAGG CCACGGCGGG
    TGGAACCCCA GGATGGCGGA GTTTATCGGA CAGACGGGCA CCGTGCACCG CATCACGGAC
    CGTGGGGATG TGCGTGTGCA GTTCGGCCGT GAGACCCGCT GGACCTTCCA CCCTGGGGCT
    CTCACCAAGC ACAATGCCTT CTGGGTGGGC GATGTGGTAC GGGTCATCGA TGACCTTGAC
    ACAGTGAAGC GGCTGCAGGC TGGGCATGGC GAGTGGACAG ATGACATGGC CCCCGCCCTG
    GGCCGCATTG GGAAGGTGGT GAAAGTTTTC AGAGACGGTA ACCTGCGTGT GGCAGTCGGT
    GGTCAGCTGT GGACCTTCAG CCCCTCCTGC CTGGTGGCCT ACCGGCCTGA GGAGGATGCC
    AACCTGGATG TGGCCGAACG TGCCAGGGAG AACAAAAGCT TCCTGAGTGT TGCTCTGGAG
    AAGCTTCGAG CCCAGAAGAG TGACCTGGAG CACCCAGGGA GACTGGTGGT GGAGGTGGCC
    TTGGGCAGTA TGGCTGGGGC TCTGGACCAG CTGAGGCGGC ACCCAGAGCA GGTGGACACC
    AAGAACCAGG GCAGGACCGC TCTGCAGGTG GCCGCCTACC TAGGCCAGGT GGAGCTGGTG
    CGGCTGCTGC TGCAGGCACG AGCGGGCATG GACCTGGCAG ATGATGAGGG CAATACGGCG
    CTGCACTATG CAGCCTTGGG GAACCAGCCT GAGGCTGCCC GGTTGCTCCT GAGCTCCGGG
    TGTGGGGCCA ATGCTCTTAA CAGCACCCGG AGCGCAGCAC TGCATGTGGC TGTGCAGAGG
    GGCTTCCTGG AGGTGGTGAA GGTCCTCTGT GAACGTGGCT GTGACGTCAA CCTGCCTGAT
    GCACACGCGG ACACACCTCT GCACTGTGCC ATCTCGGCGG GCGCCGGCGC CAGCGGCATC
    GTGGAGGTCC TCACTGAGGT GCCAGGTGTT GATGTCACGG CCACTAACAG CCAGGGCTTC
    ACCCTGCTGC ATCACGCATC CCTCAAGGGC CACACGCTAG CTGTCAGGAG GATTCTAGCT
    CGGGCACGAC AGCTGGTGGA CGCCAAGAAG GAGGATGGCT TCACAGCCTT ACACCTGGCT
    GCCCTCAACA ACCACCGGGA AGTGGCCCAG ATTCTGATCC GAGAGGGCCG CTGTGATGTG
    AACGTTCGCA ACCGTAAGCT CCAGTCCCCC CTGCACCTGG CCGTGCAGCA GGCTCATGTG
    GGGCTGGTGC CGCTGCTAGT GGACGCGGGC TGCAGTGTCA ACGCCGAGGA TGAGGAAGGG
    GACACAGCCT TGCACGTGGC CCTGCAGCGT CATCAGCTGC TGCCCCTGGC AGCAGATGGG
    GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG CTGTCCAGGC TCCAGGCCTC AGGCCTCCCG
    GGCAGCGGGG AGCTGACGGT GGGAGCGGCG GTCGCATGCT TCCTGGCGCT GGAGGGCGCC
    GACGTGAGCT ACGCCAACCA CCGCGGCCGC AGCCCGCTGG ACCTGGCCGC CGAGGGCCGT
    GTACTCAAGG CCCTGCAGGG CTGTGCGCAG CGCTTCCGGG AGAGGCACGC GGGCGGCAGC
    GGGGGTGCGG CCCCGGGCCC GAGGCTGGTG CTTGGCACCC CCAACACCGT GACGAACCTG
    CACGTGGCTG CGCCGTCGGG GCCCGAGGCC GCCGAGTGCC TGGTGTGCTC GGAGCTGGCG
    CTGCTGGTGC TGTTCTCGCC GTGCCAGCAC CGCACTGTGT GCGAGGAGTG CGCTCGCAGG
    ATGAAGAAGT GCATCAGATG CCAGGTGGTC ATCGGCAAGA AGCTGCGACC AGACGGCACA
    GAGGTGGCCA GCGCGACCCC CGCGTCCGGC CCGCCGCGGC AGCTGGTGGA GGAGCTGCAG
    AGTCGCTACC GGCAGATGGA GGAGCGCATC ACCTGCCCCA TCTGCATCGA CAGCCACATC
    CGCCTCGTGT TCCAGTGCGG CCACGGCGCG TGCGCACCCT GCGGCGCCGC GCTCAGCGCC
    TGCCCCATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA TCTTCGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022274616.1
    CDS359..2929
    Translation

    Target ORF information:

    RefSeq Version XM_022418908.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022418908.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGCGCTGGA AGTGCCGCGT CTGCTTCGAC TACGACCTGT GCACGCAGTG CTACATGCAC 
    AACAAGCATG ACCTCACCCA CGCCTTCGAG CGCTACGAGA CAGCCCACTC CCGCCCGGTC
    ACGCTGAGTC CCCGCCAGGG CCTCCCGAGG ATCCCATTGA GGGGCATCTT CCAGGGGGCG
    AAGGTGGTGC GGGGCCCTGA CTGGGAGTGG GGTTCGCAGG ATGGAGGGGA AGGGAAGCTG
    GGCCGAGTGG TGGACATCCG TGGCTGGGAT GTGGAGACGG GCCGGAGTGT GGCCAGTGTG
    ACGTGGGCTG ATGGCACCAC CAATGTGTAC CGTGTGGGTC ACAAGGGCAA GGTGGACCTC
    AAGTGTGTGA ACGAGGCAGC TGGTGGCTTC TACTACAAGG AGCACCTCCC GAAGCTTGGT
    AAGCCAGCAG AGCTGCAGCG CAGGGTGAGT GCCGACAGCC AGCCTTTCCA GCACGGGGAC
    AAGGTTAAGT GTCTGCTGGA CACAGACATC CTGAGGGAGA TGCAGGAAGG CCACGGCGGG
    TGGAACCCCA GGATGGCGGA GTTTATCGGA CAGACGGGCA CCGTGCACCG CATCACGGAC
    CGTGGGGATG TGCGTGTGCA GTTCGGCCGT GAGACCCGCT GGACCTTCCA CCCTGGGGCT
    CTCACCAAGC ACAATGCCTT CTGGGTGGGC GATGTGGTAC GGGTCATCGA TGACCTTGAC
    ACAGTGAAGC GGCTGCAGGC TGGGCATGGC GAGTGGACAG ATGACATGGC CCCCGCCCTG
    GGCCGCATTG GGAAGGTGGT GAAAGTTTTC AGAGACGGTA ACCTGCGTGT GGCAGTCGGT
    GGTCAGCTGT GGACCTTCAG CCCCTCCTGC CTGGTGGCCT ACCGGCCTGA GGAGGATGCC
    AACCTGGATG TGGCCGAACG TGCCAGGGAG AACAAAAGCT TCCTGAGTGT TGCTCTGGAG
    AAGCTTCGAG CCCAGAAGAG TGACCTGGAG CACCCAGGGA GACTGGTGGT GGAGGTGGCC
    TTGGGCAGTA TGGCTGGGGC TCTGGACCAG CTGAGGCGGC ACCCAGAGCA GGTGGACACC
    AAGAACCAGG GCAGGACCGC TCTGCAGGTG GCCGCCTACC TAGGCCAGGT GGAGCTGGTG
    CGGCTGCTGC TGCAGGCACG AGCGGGCATG GACCTGGCAG ATGATGAGGG CAATACGGCG
    CTGCACTATG CAGCCTTGGG GAACCAGCCT GAGGCTGCCC GGTTGCTCCT GAGCTCCGGG
    TGTGGGGCCA ATGCTCTTAA CAGCACCCGG AGCGCAGCAC TGCATGTGGC TGTGCAGAGG
    GGCTTCCTGG AGGTGGTGAA GGTCCTCTGT GAACGTGGCT GTGACGTCAA CCTGCCTGAT
    GCACACGCGG ACACACCTCT GCACTGTGCC ATCTCGGCGG GCGCCGGCGC CAGCGGCATC
    GTGGAGGTCC TCACTGAGGT GCCAGGTGTT GATGTCACGG CCACTAACAG CCAGGGCTTC
    ACCCTGCTGC ATCACGCATC CCTCAAGGGC CACACGCTAG CTGTCAGGAG GATTCTAGCT
    CGGGCACGAC AGCTGGTGGA CGCCAAGAAG GAGGATGGCT TCACAGCCTT ACACCTGGCT
    GCCCTCAACA ACCACCGGGA AGTGGCCCAG ATTCTGATCC GAGAGGGCCG CTGTGATGTG
    AACGTTCGCA ACCGTAAGCT CCAGTCCCCC CTGCACCTGG CCGTGCAGCA GGCTCATGTG
    GGGCTGGTGC CGCTGCTAGT GGACGCGGGC TGCAGTGTCA ACGCCGAGGA TGAGGAAGGG
    GACACAGCCT TGCACGTGGC CCTGCAGCGT CATCAGCTGC TGCCCCTGGC AGCAGATGGG
    GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG CTGTCCAGGC TCCAGGCCTC AGGCCTCCCG
    GGCAGCGGGG AGCTGACGGT GGGAGCGGCG GTCGCATGCT TCCTGGCGCT GGAGGGCGCC
    GACGTGAGCT ACGCCAACCA CCGCGGCCGC AGCCCGCTGG ACCTGGCCGC CGAGGGCCGT
    GTACTCAAGG CCCTGCAGGG CTGTGCGCAG CGCTTCCGGG AGAGGCACGC GGGCGGCAGC
    GGGGGTGCGG CCCCGGGCCC GAGGCTGGTG CTTGGCACCC CCAACACCGT GACGAACCTG
    CACGTGGCTG CGCCGTCGGG GCCCGAGGCC GCCGAGTGCC TGGTGTGCTC GGAGCTGGCG
    CTGCTGGTGC TGTTCTCGCC GTGCCAGCAC CGCACTGTGT GCGAGGAGTG CGCTCGCAGG
    ATGAAGAAGT GCATCAGATG CCAGGTGGTC ATCGGCAAGA AGCTGCGACC AGACGGCACA
    GAGGTGGCCA GCGCGACCCC CGCGTCCGGC CCGCCGCGGC AGCTGGTGGA GGAGCTGCAG
    AGTCGCTACC GGCAGATGGA GGAGCGCATC ACCTGCCCCA TCTGCATCGA CAGCCACATC
    CGCCTCGTGT TCCAGTGCGG CCACGGCGCG TGCGCACCCT GCGGCGCCGC GCTCAGCGCC
    TGCCCCATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA TCTTCGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36586
    Clone ID Related Accession (Same CDS sequence) XM_014113812.2 , XM_014113812.1
    Accession Version XM_014113812.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2877bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113812.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGCAGTG CCCCTTCCCT GCCTATCCTG TGCTGGACAT GGGTCTTGGT GTGGACCTGG 
    GCAGACGGTG GATGTCAGAG GGCCCGTGGG GCTCGGCCCC TGCTCCTGGC AAAACCTGGC
    CACTGCCCCT CCTCACTGCT CACCTGTGGC CTCAGCTGGC TGGCAGTGTT TCCTGAGGGA
    GCCAGGCCTG TGATATGGTC CCGGAGCAGT CCTGCCCACC ATGGACCCAG ACCCCCAGGC
    AGGTGTGCAG GCGTCCGCCA CCCCAACATC ATCTGCGACT GCTGCAAGAA GCACGGCCTG
    CGAGGCATGC GCTGGAAGTG CCGCGTCTGC TTCGACTACG ACCTGTGCAC GCAGTGCTAC
    ATGCACAACA AGCATGACCT CACCCACGCC TTCGAGCGCT ACGAGACAGC CCACTCCCGC
    CCGGTCACGC TGAGTCCCCG CCAGGGCCTC CCGAGGATCC CATTGAGGGG CATCTTCCAG
    GGGGCGAAGG TGGTGCGGGG CCCTGACTGG GAGTGGGGTT CGCAGGATGG AGGGGAAGGG
    AAGCTGGGCC GAGTGGTGGA CATCCGTGGC TGGGATGTGG AGACGGGCCG GAGTGTGGCC
    AGTGTGACGT GGGCTGATGG CACCACCAAT GTGTACCGTG TGGGTCACAA GGGCAAGGTG
    GACCTCAAGT GTGTGAACGA GGCAGCTGGT GGCTTCTACT ACAAGGAGCA CCTCCCGAAG
    CTTGGTAAGC CAGCAGAGCT GCAGCGCAGG GTGAGTGCCG ACAGCCAGCC TTTCCAGCAC
    GGGGACAAGG TTAAGTGTCT GCTGGACACA GACATCCTGA GGGAGATGCA GGAAGGCCAC
    GGCGGGTGGA ACCCCAGGAT GGCGGAGTTT ATCGGACAGA CGGGCACCGT GCACCGCATC
    ACGGACCGTG GGGATGTGCG TGTGCAGTTC GGCCGTGAGA CCCGCTGGAC CTTCCACCCT
    GGGGCTCTCA CCAAGCACAA TGCCTTCTGG GTGGGCGATG TGGTACGGGT CATCGATGAC
    CTTGACACAG TGAAGCGGCT GCAGGCTGGG CATGGCGAGT GGACAGATGA CATGGCCCCC
    GCCCTGGGCC GCATTGGGAA GGTGGTGAAA GTTTTCAGAG ACGGTAACCT GCGTGTGGCA
    GTCGGTGGTC AGCTGTGGAC CTTCAGCCCC TCCTGCCTGG TGGCCTACCG GCCTGAGGAG
    GATGCCAACC TGGATGTGGC CGAACGTGCC AGGGAGAACA AAAGCTTCCT GAGTGTTGCT
    CTGGAGAAGC TTCGAGCCCA GAAGAGTGAC CTGGAGCACC CAGGGAGACT GGTGGTGGAG
    GTGGCCTTGG GCAGTATGGC TGGGGCTCTG GACCAGCTGA GGCGGCACCC AGAGCAGGTG
    GACACCAAGA ACCAGGGCAG GACCGCTCTG CAGGTGGCCG CCTACCTAGG CCAGGTGGAG
    CTGGTGCGGC TGCTGCTGCA GGCACGAGCG GGCATGGACC TGGCAGATGA TGAGGGCAAT
    ACGGCGCTGC ACTATGCAGC CTTGGGGAAC CAGCCTGAGG CTGCCCGGTT GCTCCTGAGC
    TCCGGGTGTG GGGCCAATGC TCTTAACAGC ACCCGGAGCG CAGCACTGCA TGTGGCTGTG
    CAGAGGGGCT TCCTGGAGGT GGTGAAGGTC CTCTGTGAAC GTGGCTGTGA CGTCAACCTG
    CCTGATGCAC ACGCGGACAC ACCTCTGCAC TGTGCCATCT CGGCGGGCGC CGGCGCCAGC
    GGCATCGTGG AGGTCCTCAC TGAGGTGCCA GGTGTTGATG TCACGGCCAC TAACAGCCAG
    GGCTTCACCC TGCTGCATCA CGCATCCCTC AAGGGCCACA CGCTAGCTGT CAGGAGGATT
    CTAGCTCGGG CACGACAGCT GGTGGACGCC AAGAAGGAGG ATGGCTTCAC AGCCTTACAC
    CTGGCTGCCC TCAACAACCA CCGGGAAGTG GCCCAGATTC TGATCCGAGA GGGCCGCTGT
    GATGTGAACG TTCGCAACCG TAAGCTCCAG TCCCCCCTGC ACCTGGCCGT GCAGCAGGCT
    CATGTGGGGC TGGTGCCGCT GCTAGTGGAC GCGGGCTGCA GTGTCAACGC CGAGGATGAG
    GAAGGGGACA CAGCCTTGCA CGTGGCCCTG CAGCGTCATC AGCTGCTGCC CCTGGCAGCA
    GATGGGGCCG GGGGGGACCC AGGGCCCTTG CAGCTGCTGT CCAGGCTCCA GGCCTCAGGC
    CTCCCGGGCA GCGGGGAGCT GACGGTGGGA GCGGCGGTCG CATGCTTCCT GGCGCTGGAG
    GGCGCCGACG TGAGCTACGC CAACCACCGC GGCCGCAGCC CGCTGGACCT GGCCGCCGAG
    GGCCGTGTAC TCAAGGCCCT GCAGGGCTGT GCGCAGCGCT TCCGGGAGAG GCACGCGGGC
    GGCAGCGGGG GTGCGGCCCC GGGCCCGAGG CTGGTGCTTG GCACCCCCAA CACCGTGACG
    AACCTGCACG TGGCTGCGCC GTCGGGGCCC GAGGCCGCCG AGTGCCTGGT GTGCTCGGAG
    CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC CAGCACCGCA CTGTGTGCGA GGAGTGCGCT
    CGCAGGATGA AGAAGTGCAT CAGATGCCAG GTGGTCATCG GCAAGAAGCT GCGACCAGAC
    GGCACAGAGG TGGCCAGCGC GACCCCCGCG TCCGGCCCGC CGCGGCAGCT GGTGGAGGAG
    CTGCAGAGTC GCTACCGGCA GATGGAGGAG CGCATCACCT GCCCCATCTG CATCGACAGC
    CACATCCGCC TCGTGTTCCA GTGCGGCCAC GGCGCGTGCG CACCCTGCGG CGCCGCGCTC
    AGCGCCTGCC CCATCTGCCG CCAGCCCATC CGCGACCGCA TCCAGATCTT CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969287.1
    CDS454..3330
    Translation

    Target ORF information:

    RefSeq Version XM_014113812.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113812.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGCAGTG CCCCTTCCCT GCCTATCCTG TGCTGGACAT GGGTCTTGGT GTGGACCTGG 
    GCAGACGGTG GATGTCAGAG GGCCCGTGGG GCTCGGCCCC TGCTCCTGGC AAAACCTGGC
    CACTGCCCCT CCTCACTGCT CACCTGTGGC CTCAGCTGGC TGGCAGTGTT TCCTGAGGGA
    GCCAGGCCTG TGATATGGTC CCGGAGCAGT CCTGCCCACC ATGGACCCAG ACCCCCAGGC
    AGGTGTGCAG GCGTCCGCCA CCCCAACATC ATCTGCGACT GCTGCAAGAA GCACGGCCTG
    CGAGGCATGC GCTGGAAGTG CCGCGTCTGC TTCGACTACG ACCTGTGCAC GCAGTGCTAC
    ATGCACAACA AGCATGACCT CACCCACGCC TTCGAGCGCT ACGAGACAGC CCACTCCCGC
    CCGGTCACGC TGAGTCCCCG CCAGGGCCTC CCGAGGATCC CATTGAGGGG CATCTTCCAG
    GGGGCGAAGG TGGTGCGGGG CCCTGACTGG GAGTGGGGTT CGCAGGATGG AGGGGAAGGG
    AAGCTGGGCC GAGTGGTGGA CATCCGTGGC TGGGATGTGG AGACGGGCCG GAGTGTGGCC
    AGTGTGACGT GGGCTGATGG CACCACCAAT GTGTACCGTG TGGGTCACAA GGGCAAGGTG
    GACCTCAAGT GTGTGAACGA GGCAGCTGGT GGCTTCTACT ACAAGGAGCA CCTCCCGAAG
    CTTGGTAAGC CAGCAGAGCT GCAGCGCAGG GTGAGTGCCG ACAGCCAGCC TTTCCAGCAC
    GGGGACAAGG TTAAGTGTCT GCTGGACACA GACATCCTGA GGGAGATGCA GGAAGGCCAC
    GGCGGGTGGA ACCCCAGGAT GGCGGAGTTT ATCGGACAGA CGGGCACCGT GCACCGCATC
    ACGGACCGTG GGGATGTGCG TGTGCAGTTC GGCCGTGAGA CCCGCTGGAC CTTCCACCCT
    GGGGCTCTCA CCAAGCACAA TGCCTTCTGG GTGGGCGATG TGGTACGGGT CATCGATGAC
    CTTGACACAG TGAAGCGGCT GCAGGCTGGG CATGGCGAGT GGACAGATGA CATGGCCCCC
    GCCCTGGGCC GCATTGGGAA GGTGGTGAAA GTTTTCAGAG ACGGTAACCT GCGTGTGGCA
    GTCGGTGGTC AGCTGTGGAC CTTCAGCCCC TCCTGCCTGG TGGCCTACCG GCCTGAGGAG
    GATGCCAACC TGGATGTGGC CGAACGTGCC AGGGAGAACA AAAGCTTCCT GAGTGTTGCT
    CTGGAGAAGC TTCGAGCCCA GAAGAGTGAC CTGGAGCACC CAGGGAGACT GGTGGTGGAG
    GTGGCCTTGG GCAGTATGGC TGGGGCTCTG GACCAGCTGA GGCGGCACCC AGAGCAGGTG
    GACACCAAGA ACCAGGGCAG GACCGCTCTG CAGGTGGCCG CCTACCTAGG CCAGGTGGAG
    CTGGTGCGGC TGCTGCTGCA GGCACGAGCG GGCATGGACC TGGCAGATGA TGAGGGCAAT
    ACGGCGCTGC ACTATGCAGC CTTGGGGAAC CAGCCTGAGG CTGCCCGGTT GCTCCTGAGC
    TCCGGGTGTG GGGCCAATGC TCTTAACAGC ACCCGGAGCG CAGCACTGCA TGTGGCTGTG
    CAGAGGGGCT TCCTGGAGGT GGTGAAGGTC CTCTGTGAAC GTGGCTGTGA CGTCAACCTG
    CCTGATGCAC ACGCGGACAC ACCTCTGCAC TGTGCCATCT CGGCGGGCGC CGGCGCCAGC
    GGCATCGTGG AGGTCCTCAC TGAGGTGCCA GGTGTTGATG TCACGGCCAC TAACAGCCAG
    GGCTTCACCC TGCTGCATCA CGCATCCCTC AAGGGCCACA CGCTAGCTGT CAGGAGGATT
    CTAGCTCGGG CACGACAGCT GGTGGACGCC AAGAAGGAGG ATGGCTTCAC AGCCTTACAC
    CTGGCTGCCC TCAACAACCA CCGGGAAGTG GCCCAGATTC TGATCCGAGA GGGCCGCTGT
    GATGTGAACG TTCGCAACCG TAAGCTCCAG TCCCCCCTGC ACCTGGCCGT GCAGCAGGCT
    CATGTGGGGC TGGTGCCGCT GCTAGTGGAC GCGGGCTGCA GTGTCAACGC CGAGGATGAG
    GAAGGGGACA CAGCCTTGCA CGTGGCCCTG CAGCGTCATC AGCTGCTGCC CCTGGCAGCA
    GATGGGGCCG GGGGGGACCC AGGGCCCTTG CAGCTGCTGT CCAGGCTCCA GGCCTCAGGC
    CTCCCGGGCA GCGGGGAGCT GACGGTGGGA GCGGCGGTCG CATGCTTCCT GGCGCTGGAG
    GGCGCCGACG TGAGCTACGC CAACCACCGC GGCCGCAGCC CGCTGGACCT GGCCGCCGAG
    GGCCGTGTAC TCAAGGCCCT GCAGGGCTGT GCGCAGCGCT TCCGGGAGAG GCACGCGGGC
    GGCAGCGGGG GTGCGGCCCC GGGCCCGAGG CTGGTGCTTG GCACCCCCAA CACCGTGACG
    AACCTGCACG TGGCTGCGCC GTCGGGGCCC GAGGCCGCCG AGTGCCTGGT GTGCTCGGAG
    CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC CAGCACCGCA CTGTGTGCGA GGAGTGCGCT
    CGCAGGATGA AGAAGTGCAT CAGATGCCAG GTGGTCATCG GCAAGAAGCT GCGACCAGAC
    GGCACAGAGG TGGCCAGCGC GACCCCCGCG TCCGGCCCGC CGCGGCAGCT GGTGGAGGAG
    CTGCAGAGTC GCTACCGGCA GATGGAGGAG CGCATCACCT GCCCCATCTG CATCGACAGC
    CACATCCGCC TCGTGTTCCA GTGCGGCCAC GGCGCGTGCG CACCCTGCGG CGCCGCGCTC
    AGCGCCTGCC CCATCTGCCG CCAGCCCATC CGCGACCGCA TCCAGATCTT CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36586
    Clone ID Related Accession (Same CDS sequence) XM_014113812.2 , XM_014113812.1
    Accession Version XM_014113812.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2877bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGCAGTG CCCCTTCCCT GCCTATCCTG TGCTGGACAT GGGTCTTGGT GTGGACCTGG 
    GCAGACGGTG GATGTCAGAG GGCCCGTGGG GCTCGGCCCC TGCTCCTGGC AAAACCTGGC
    CACTGCCCCT CCTCACTGCT CACCTGTGGC CTCAGCTGGC TGGCAGTGTT TCCTGAGGGA
    GCCAGGCCTG TGATATGGTC CCGGAGCAGT CCTGCCCACC ATGGACCCAG ACCCCCAGGC
    AGGTGTGCAG GCGTCCGCCA CCCCAACATC ATCTGCGACT GCTGCAAGAA GCACGGCCTG
    CGAGGCATGC GCTGGAAGTG CCGCGTCTGC TTCGACTACG ACCTGTGCAC GCAGTGCTAC
    ATGCACAACA AGCATGACCT CACCCACGCC TTCGAGCGCT ACGAGACAGC CCACTCCCGC
    CCGGTCACGC TGAGTCCCCG CCAGGGCCTC CCGAGGATCC CATTGAGGGG CATCTTCCAG
    GGGGCGAAGG TGGTGCGGGG CCCTGACTGG GAGTGGGGTT CGCAGGATGG AGGGGAAGGG
    AAGCTGGGCC GAGTGGTGGA CATCCGTGGC TGGGATGTGG AGACGGGCCG GAGTGTGGCC
    AGTGTGACGT GGGCTGATGG CACCACCAAT GTGTACCGTG TGGGTCACAA GGGCAAGGTG
    GACCTCAAGT GTGTGAACGA GGCAGCTGGT GGCTTCTACT ACAAGGAGCA CCTCCCGAAG
    CTTGGTAAGC CAGCAGAGCT GCAGCGCAGG GTGAGTGCCG ACAGCCAGCC TTTCCAGCAC
    GGGGACAAGG TTAAGTGTCT GCTGGACACA GACATCCTGA GGGAGATGCA GGAAGGCCAC
    GGCGGGTGGA ACCCCAGGAT GGCGGAGTTT ATCGGACAGA CGGGCACCGT GCACCGCATC
    ACGGACCGTG GGGATGTGCG TGTGCAGTTC GGCCGTGAGA CCCGCTGGAC CTTCCACCCT
    GGGGCTCTCA CCAAGCACAA TGCCTTCTGG GTGGGCGATG TGGTACGGGT CATCGATGAC
    CTTGACACAG TGAAGCGGCT GCAGGCTGGG CATGGCGAGT GGACAGATGA CATGGCCCCC
    GCCCTGGGCC GCATTGGGAA GGTGGTGAAA GTTTTCAGAG ACGGTAACCT GCGTGTGGCA
    GTCGGTGGTC AGCTGTGGAC CTTCAGCCCC TCCTGCCTGG TGGCCTACCG GCCTGAGGAG
    GATGCCAACC TGGATGTGGC CGAACGTGCC AGGGAGAACA AAAGCTTCCT GAGTGTTGCT
    CTGGAGAAGC TTCGAGCCCA GAAGAGTGAC CTGGAGCACC CAGGGAGACT GGTGGTGGAG
    GTGGCCTTGG GCAGTATGGC TGGGGCTCTG GACCAGCTGA GGCGGCACCC AGAGCAGGTG
    GACACCAAGA ACCAGGGCAG GACCGCTCTG CAGGTGGCCG CCTACCTAGG CCAGGTGGAG
    CTGGTGCGGC TGCTGCTGCA GGCACGAGCG GGCATGGACC TGGCAGATGA TGAGGGCAAT
    ACGGCGCTGC ACTATGCAGC CTTGGGGAAC CAGCCTGAGG CTGCCCGGTT GCTCCTGAGC
    TCCGGGTGTG GGGCCAATGC TCTTAACAGC ACCCGGAGCG CAGCACTGCA TGTGGCTGTG
    CAGAGGGGCT TCCTGGAGGT GGTGAAGGTC CTCTGTGAAC GTGGCTGTGA CGTCAACCTG
    CCTGATGCAC ACGCGGACAC ACCTCTGCAC TGTGCCATCT CGGCGGGCGC CGGCGCCAGC
    GGCATCGTGG AGGTCCTCAC TGAGGTGCCA GGTGTTGATG TCACGGCCAC TAACAGCCAG
    GGCTTCACCC TGCTGCATCA CGCATCCCTC AAGGGCCACA CGCTAGCTGT CAGGAGGATT
    CTAGCTCGGG CACGACAGCT GGTGGACGCC AAGAAGGAGG ATGGCTTCAC AGCCTTACAC
    CTGGCTGCCC TCAACAACCA CCGGGAAGTG GCCCAGATTC TGATCCGAGA GGGCCGCTGT
    GATGTGAACG TTCGCAACCG TAAGCTCCAG TCCCCCCTGC ACCTGGCCGT GCAGCAGGCT
    CATGTGGGGC TGGTGCCGCT GCTAGTGGAC GCGGGCTGCA GTGTCAACGC CGAGGATGAG
    GAAGGGGACA CAGCCTTGCA CGTGGCCCTG CAGCGTCATC AGCTGCTGCC CCTGGCAGCA
    GATGGGGCCG GGGGGGACCC AGGGCCCTTG CAGCTGCTGT CCAGGCTCCA GGCCTCAGGC
    CTCCCGGGCA GCGGGGAGCT GACGGTGGGA GCGGCGGTCG CATGCTTCCT GGCGCTGGAG
    GGCGCCGACG TGAGCTACGC CAACCACCGC GGCCGCAGCC CGCTGGACCT GGCCGCCGAG
    GGCCGTGTAC TCAAGGCCCT GCAGGGCTGT GCGCAGCGCT TCCGGGAGAG GCACGCGGGC
    GGCAGCGGGG GTGCGGCCCC GGGCCCGAGG CTGGTGCTTG GCACCCCCAA CACCGTGACG
    AACCTGCACG TGGCTGCGCC GTCGGGGCCC GAGGCCGCCG AGTGCCTGGT GTGCTCGGAG
    CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC CAGCACCGCA CTGTGTGCGA GGAGTGCGCT
    CGCAGGATGA AGAAGTGCAT CAGATGCCAG GTGGTCATCG GCAAGAAGCT GCGACCAGAC
    GGCACAGAGG TGGCCAGCGC GACCCCCGCG TCCGGCCCGC CGCGGCAGCT GGTGGAGGAG
    CTGCAGAGTC GCTACCGGCA GATGGAGGAG CGCATCACCT GCCCCATCTG CATCGACAGC
    CACATCCGCC TCGTGTTCCA GTGCGGCCAC GGCGCGTGCG CACCCTGCGG CGCCGCGCTC
    AGCGCCTGCC CCATCTGCCG CCAGCCCATC CGCGACCGCA TCCAGATCTT CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969287.1
    CDS1898..4774
    Translation

    Target ORF information:

    RefSeq Version XM_014113812.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113812.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGCAGTG CCCCTTCCCT GCCTATCCTG TGCTGGACAT GGGTCTTGGT GTGGACCTGG 
    GCAGACGGTG GATGTCAGAG GGCCCGTGGG GCTCGGCCCC TGCTCCTGGC AAAACCTGGC
    CACTGCCCCT CCTCACTGCT CACCTGTGGC CTCAGCTGGC TGGCAGTGTT TCCTGAGGGA
    GCCAGGCCTG TGATATGGTC CCGGAGCAGT CCTGCCCACC ATGGACCCAG ACCCCCAGGC
    AGGTGTGCAG GCGTCCGCCA CCCCAACATC ATCTGCGACT GCTGCAAGAA GCACGGCCTG
    CGAGGCATGC GCTGGAAGTG CCGCGTCTGC TTCGACTACG ACCTGTGCAC GCAGTGCTAC
    ATGCACAACA AGCATGACCT CACCCACGCC TTCGAGCGCT ACGAGACAGC CCACTCCCGC
    CCGGTCACGC TGAGTCCCCG CCAGGGCCTC CCGAGGATCC CATTGAGGGG CATCTTCCAG
    GGGGCGAAGG TGGTGCGGGG CCCTGACTGG GAGTGGGGTT CGCAGGATGG AGGGGAAGGG
    AAGCTGGGCC GAGTGGTGGA CATCCGTGGC TGGGATGTGG AGACGGGCCG GAGTGTGGCC
    AGTGTGACGT GGGCTGATGG CACCACCAAT GTGTACCGTG TGGGTCACAA GGGCAAGGTG
    GACCTCAAGT GTGTGAACGA GGCAGCTGGT GGCTTCTACT ACAAGGAGCA CCTCCCGAAG
    CTTGGTAAGC CAGCAGAGCT GCAGCGCAGG GTGAGTGCCG ACAGCCAGCC TTTCCAGCAC
    GGGGACAAGG TTAAGTGTCT GCTGGACACA GACATCCTGA GGGAGATGCA GGAAGGCCAC
    GGCGGGTGGA ACCCCAGGAT GGCGGAGTTT ATCGGACAGA CGGGCACCGT GCACCGCATC
    ACGGACCGTG GGGATGTGCG TGTGCAGTTC GGCCGTGAGA CCCGCTGGAC CTTCCACCCT
    GGGGCTCTCA CCAAGCACAA TGCCTTCTGG GTGGGCGATG TGGTACGGGT CATCGATGAC
    CTTGACACAG TGAAGCGGCT GCAGGCTGGG CATGGCGAGT GGACAGATGA CATGGCCCCC
    GCCCTGGGCC GCATTGGGAA GGTGGTGAAA GTTTTCAGAG ACGGTAACCT GCGTGTGGCA
    GTCGGTGGTC AGCTGTGGAC CTTCAGCCCC TCCTGCCTGG TGGCCTACCG GCCTGAGGAG
    GATGCCAACC TGGATGTGGC CGAACGTGCC AGGGAGAACA AAAGCTTCCT GAGTGTTGCT
    CTGGAGAAGC TTCGAGCCCA GAAGAGTGAC CTGGAGCACC CAGGGAGACT GGTGGTGGAG
    GTGGCCTTGG GCAGTATGGC TGGGGCTCTG GACCAGCTGA GGCGGCACCC AGAGCAGGTG
    GACACCAAGA ACCAGGGCAG GACCGCTCTG CAGGTGGCCG CCTACCTAGG CCAGGTGGAG
    CTGGTGCGGC TGCTGCTGCA GGCACGAGCG GGCATGGACC TGGCAGATGA TGAGGGCAAT
    ACGGCGCTGC ACTATGCAGC CTTGGGGAAC CAGCCTGAGG CTGCCCGGTT GCTCCTGAGC
    TCCGGGTGTG GGGCCAATGC TCTTAACAGC ACCCGGAGCG CAGCACTGCA TGTGGCTGTG
    CAGAGGGGCT TCCTGGAGGT GGTGAAGGTC CTCTGTGAAC GTGGCTGTGA CGTCAACCTG
    CCTGATGCAC ACGCGGACAC ACCTCTGCAC TGTGCCATCT CGGCGGGCGC CGGCGCCAGC
    GGCATCGTGG AGGTCCTCAC TGAGGTGCCA GGTGTTGATG TCACGGCCAC TAACAGCCAG
    GGCTTCACCC TGCTGCATCA CGCATCCCTC AAGGGCCACA CGCTAGCTGT CAGGAGGATT
    CTAGCTCGGG CACGACAGCT GGTGGACGCC AAGAAGGAGG ATGGCTTCAC AGCCTTACAC
    CTGGCTGCCC TCAACAACCA CCGGGAAGTG GCCCAGATTC TGATCCGAGA GGGCCGCTGT
    GATGTGAACG TTCGCAACCG TAAGCTCCAG TCCCCCCTGC ACCTGGCCGT GCAGCAGGCT
    CATGTGGGGC TGGTGCCGCT GCTAGTGGAC GCGGGCTGCA GTGTCAACGC CGAGGATGAG
    GAAGGGGACA CAGCCTTGCA CGTGGCCCTG CAGCGTCATC AGCTGCTGCC CCTGGCAGCA
    GATGGGGCCG GGGGGGACCC AGGGCCCTTG CAGCTGCTGT CCAGGCTCCA GGCCTCAGGC
    CTCCCGGGCA GCGGGGAGCT GACGGTGGGA GCGGCGGTCG CATGCTTCCT GGCGCTGGAG
    GGCGCCGACG TGAGCTACGC CAACCACCGC GGCCGCAGCC CGCTGGACCT GGCCGCCGAG
    GGCCGTGTAC TCAAGGCCCT GCAGGGCTGT GCGCAGCGCT TCCGGGAGAG GCACGCGGGC
    GGCAGCGGGG GTGCGGCCCC GGGCCCGAGG CTGGTGCTTG GCACCCCCAA CACCGTGACG
    AACCTGCACG TGGCTGCGCC GTCGGGGCCC GAGGCCGCCG AGTGCCTGGT GTGCTCGGAG
    CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC CAGCACCGCA CTGTGTGCGA GGAGTGCGCT
    CGCAGGATGA AGAAGTGCAT CAGATGCCAG GTGGTCATCG GCAAGAAGCT GCGACCAGAC
    GGCACAGAGG TGGCCAGCGC GACCCCCGCG TCCGGCCCGC CGCGGCAGCT GGTGGAGGAG
    CTGCAGAGTC GCTACCGGCA GATGGAGGAG CGCATCACCT GCCCCATCTG CATCGACAGC
    CACATCCGCC TCGTGTTCCA GTGCGGCCAC GGCGCGTGCG CACCCTGCGG CGCCGCGCTC
    AGCGCCTGCC CCATCTGCCG CCAGCCCATC CGCGACCGCA TCCAGATCTT CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36587
    Clone ID Related Accession (Same CDS sequence) XM_014113816.2 , XM_014113816.1
    Accession Version XM_014113816.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2865bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006587.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014113816.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGCAGTG CCCCTTCCCT GCCTATCCTG TGCTGGACAT GGGTCTTGGT GTGGACCTGG 
    GCAGACGGTG GATGTCAGAG GGCCCGTGGG GCTCGGCCCC TGCTCCTGGC AAAACCTGGC
    CACTGCCCCT CCTCACTGCT CACCTGTGGC CTCAGCTGGC TGGCAGTGTT TCCTGAGGGA
    GCCAGGCCTG TGATATGGTC CCGGAGCAGT CCTGCCCACC ATGGACCCAG ACCCCCAGGC
    AGGTGTGCAG GCGTCCGCCA CCCCAACATC ATCTGCGACT GCTGCAAGAA GCACGGCCTG
    CGAGGCATGC GCTGGAAGTG CCGCGTCTGC TTCGACTACG ACCTGTGCAC GCAGTGCTAC
    ATGCACAACA AGCATGACCT CACCCACGCC TTCGAGCGCT ACGAGACAGC CCACTCCCGC
    CCGGTCACGC TGAGTCCCCG CCAGGGCCTC CCGAGGATCC CATTGAGGGG CATCTTCCAG
    GGGGCGAAGG TGGTGCGGGG CCCTGACTGG GAGTGGGGTT CGCAGGATGG AGGGGAAGGG
    AAGCTGGGCC GAGTGGTGGA CATCCGTGGC TGGGATGTGG AGACGGGCCG GAGTGTGGCC
    AGTGTGACGT GGGCTGATGG CACCACCAAT GTGTACCGTG TGGGTCACAA GGGCAAGGTG
    GACCTCAAGT GTGTGAACGA GGCAGCTGGT GGCTTCTACT ACAAGGAGCA CCTCCCGAAG
    CTTGGTAAGC CAGCAGAGCT GCAGCGCAGG GTGAGTGCCG ACAGCCAGCC TTTCCAGCAC
    GGGGACAAGG TTAAGTGTCT GCTGGACACA GACATCCTGA GGGAGATGCA GGAAGGCCAC
    GGCGGGTGGA ACCCCAGGAT GGCGGAGACG GGCACCGTGC ACCGCATCAC GGACCGTGGG
    GATGTGCGTG TGCAGTTCGG CCGTGAGACC CGCTGGACCT TCCACCCTGG GGCTCTCACC
    AAGCACAATG CCTTCTGGGT GGGCGATGTG GTACGGGTCA TCGATGACCT TGACACAGTG
    AAGCGGCTGC AGGCTGGGCA TGGCGAGTGG ACAGATGACA TGGCCCCCGC CCTGGGCCGC
    ATTGGGAAGG TGGTGAAAGT TTTCAGAGAC GGTAACCTGC GTGTGGCAGT CGGTGGTCAG
    CTGTGGACCT TCAGCCCCTC CTGCCTGGTG GCCTACCGGC CTGAGGAGGA TGCCAACCTG
    GATGTGGCCG AACGTGCCAG GGAGAACAAA AGCTTCCTGA GTGTTGCTCT GGAGAAGCTT
    CGAGCCCAGA AGAGTGACCT GGAGCACCCA GGGAGACTGG TGGTGGAGGT GGCCTTGGGC
    AGTATGGCTG GGGCTCTGGA CCAGCTGAGG CGGCACCCAG AGCAGGTGGA CACCAAGAAC
    CAGGGCAGGA CCGCTCTGCA GGTGGCCGCC TACCTAGGCC AGGTGGAGCT GGTGCGGCTG
    CTGCTGCAGG CACGAGCGGG CATGGACCTG GCAGATGATG AGGGCAATAC GGCGCTGCAC
    TATGCAGCCT TGGGGAACCA GCCTGAGGCT GCCCGGTTGC TCCTGAGCTC CGGGTGTGGG
    GCCAATGCTC TTAACAGCAC CCGGAGCGCA GCACTGCATG TGGCTGTGCA GAGGGGCTTC
    CTGGAGGTGG TGAAGGTCCT CTGTGAACGT GGCTGTGACG TCAACCTGCC TGATGCACAC
    GCGGACACAC CTCTGCACTG TGCCATCTCG GCGGGCGCCG GCGCCAGCGG CATCGTGGAG
    GTCCTCACTG AGGTGCCAGG TGTTGATGTC ACGGCCACTA ACAGCCAGGG CTTCACCCTG
    CTGCATCACG CATCCCTCAA GGGCCACACG CTAGCTGTCA GGAGGATTCT AGCTCGGGCA
    CGACAGCTGG TGGACGCCAA GAAGGAGGAT GGCTTCACAG CCTTACACCT GGCTGCCCTC
    AACAACCACC GGGAAGTGGC CCAGATTCTG ATCCGAGAGG GCCGCTGTGA TGTGAACGTT
    CGCAACCGTA AGCTCCAGTC CCCCCTGCAC CTGGCCGTGC AGCAGGCTCA TGTGGGGCTG
    GTGCCGCTGC TAGTGGACGC GGGCTGCAGT GTCAACGCCG AGGATGAGGA AGGGGACACA
    GCCTTGCACG TGGCCCTGCA GCGTCATCAG CTGCTGCCCC TGGCAGCAGA TGGGGCCGGG
    GGGGACCCAG GGCCCTTGCA GCTGCTGTCC AGGCTCCAGG CCTCAGGCCT CCCGGGCAGC
    GGGGAGCTGA CGGTGGGAGC GGCGGTCGCA TGCTTCCTGG CGCTGGAGGG CGCCGACGTG
    AGCTACGCCA ACCACCGCGG CCGCAGCCCG CTGGACCTGG CCGCCGAGGG CCGTGTACTC
    AAGGCCCTGC AGGGCTGTGC GCAGCGCTTC CGGGAGAGGC ACGCGGGCGG CAGCGGGGGT
    GCGGCCCCGG GCCCGAGGCT GGTGCTTGGC ACCCCCAACA CCGTGACGAA CCTGCACGTG
    GCTGCGCCGT CGGGGCCCGA GGCCGCCGAG TGCCTGGTGT GCTCGGAGCT GGCGCTGCTG
    GTGCTGTTCT CGCCGTGCCA GCACCGCACT GTGTGCGAGG AGTGCGCTCG CAGGATGAAG
    AAGTGCATCA GATGCCAGGT GGTCATCGGC AAGAAGCTGC GACCAGACGG CACAGAGGTG
    GCCAGCGCGA CCCCCGCGTC CGGCCCGCCG CGGCAGCTGG TGGAGGAGCT GCAGAGTCGC
    TACCGGCAGA TGGAGGAGCG CATCACCTGC CCCATCTGCA TCGACAGCCA CATCCGCCTC
    GTGTTCCAGT GCGGCCACGG CGCGTGCGCA CCCTGCGGCG CCGCGCTCAG CGCCTGCCCC
    ATCTGCCGCC AGCCCATCCG CGACCGCATC CAGATCTTCG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969291.1
    CDS456..3320
    Translation

    Target ORF information:

    RefSeq Version XM_014113816.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113816.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGCAGTG CCCCTTCCCT GCCTATCCTG TGCTGGACAT GGGTCTTGGT GTGGACCTGG 
    GCAGACGGTG GATGTCAGAG GGCCCGTGGG GCTCGGCCCC TGCTCCTGGC AAAACCTGGC
    CACTGCCCCT CCTCACTGCT CACCTGTGGC CTCAGCTGGC TGGCAGTGTT TCCTGAGGGA
    GCCAGGCCTG TGATATGGTC CCGGAGCAGT CCTGCCCACC ATGGACCCAG ACCCCCAGGC
    AGGTGTGCAG GCGTCCGCCA CCCCAACATC ATCTGCGACT GCTGCAAGAA GCACGGCCTG
    CGAGGCATGC GCTGGAAGTG CCGCGTCTGC TTCGACTACG ACCTGTGCAC GCAGTGCTAC
    ATGCACAACA AGCATGACCT CACCCACGCC TTCGAGCGCT ACGAGACAGC CCACTCCCGC
    CCGGTCACGC TGAGTCCCCG CCAGGGCCTC CCGAGGATCC CATTGAGGGG CATCTTCCAG
    GGGGCGAAGG TGGTGCGGGG CCCTGACTGG GAGTGGGGTT CGCAGGATGG AGGGGAAGGG
    AAGCTGGGCC GAGTGGTGGA CATCCGTGGC TGGGATGTGG AGACGGGCCG GAGTGTGGCC
    AGTGTGACGT GGGCTGATGG CACCACCAAT GTGTACCGTG TGGGTCACAA GGGCAAGGTG
    GACCTCAAGT GTGTGAACGA GGCAGCTGGT GGCTTCTACT ACAAGGAGCA CCTCCCGAAG
    CTTGGTAAGC CAGCAGAGCT GCAGCGCAGG GTGAGTGCCG ACAGCCAGCC TTTCCAGCAC
    GGGGACAAGG TTAAGTGTCT GCTGGACACA GACATCCTGA GGGAGATGCA GGAAGGCCAC
    GGCGGGTGGA ACCCCAGGAT GGCGGAGACG GGCACCGTGC ACCGCATCAC GGACCGTGGG
    GATGTGCGTG TGCAGTTCGG CCGTGAGACC CGCTGGACCT TCCACCCTGG GGCTCTCACC
    AAGCACAATG CCTTCTGGGT GGGCGATGTG GTACGGGTCA TCGATGACCT TGACACAGTG
    AAGCGGCTGC AGGCTGGGCA TGGCGAGTGG ACAGATGACA TGGCCCCCGC CCTGGGCCGC
    ATTGGGAAGG TGGTGAAAGT TTTCAGAGAC GGTAACCTGC GTGTGGCAGT CGGTGGTCAG
    CTGTGGACCT TCAGCCCCTC CTGCCTGGTG GCCTACCGGC CTGAGGAGGA TGCCAACCTG
    GATGTGGCCG AACGTGCCAG GGAGAACAAA AGCTTCCTGA GTGTTGCTCT GGAGAAGCTT
    CGAGCCCAGA AGAGTGACCT GGAGCACCCA GGGAGACTGG TGGTGGAGGT GGCCTTGGGC
    AGTATGGCTG GGGCTCTGGA CCAGCTGAGG CGGCACCCAG AGCAGGTGGA CACCAAGAAC
    CAGGGCAGGA CCGCTCTGCA GGTGGCCGCC TACCTAGGCC AGGTGGAGCT GGTGCGGCTG
    CTGCTGCAGG CACGAGCGGG CATGGACCTG GCAGATGATG AGGGCAATAC GGCGCTGCAC
    TATGCAGCCT TGGGGAACCA GCCTGAGGCT GCCCGGTTGC TCCTGAGCTC CGGGTGTGGG
    GCCAATGCTC TTAACAGCAC CCGGAGCGCA GCACTGCATG TGGCTGTGCA GAGGGGCTTC
    CTGGAGGTGG TGAAGGTCCT CTGTGAACGT GGCTGTGACG TCAACCTGCC TGATGCACAC
    GCGGACACAC CTCTGCACTG TGCCATCTCG GCGGGCGCCG GCGCCAGCGG CATCGTGGAG
    GTCCTCACTG AGGTGCCAGG TGTTGATGTC ACGGCCACTA ACAGCCAGGG CTTCACCCTG
    CTGCATCACG CATCCCTCAA GGGCCACACG CTAGCTGTCA GGAGGATTCT AGCTCGGGCA
    CGACAGCTGG TGGACGCCAA GAAGGAGGAT GGCTTCACAG CCTTACACCT GGCTGCCCTC
    AACAACCACC GGGAAGTGGC CCAGATTCTG ATCCGAGAGG GCCGCTGTGA TGTGAACGTT
    CGCAACCGTA AGCTCCAGTC CCCCCTGCAC CTGGCCGTGC AGCAGGCTCA TGTGGGGCTG
    GTGCCGCTGC TAGTGGACGC GGGCTGCAGT GTCAACGCCG AGGATGAGGA AGGGGACACA
    GCCTTGCACG TGGCCCTGCA GCGTCATCAG CTGCTGCCCC TGGCAGCAGA TGGGGCCGGG
    GGGGACCCAG GGCCCTTGCA GCTGCTGTCC AGGCTCCAGG CCTCAGGCCT CCCGGGCAGC
    GGGGAGCTGA CGGTGGGAGC GGCGGTCGCA TGCTTCCTGG CGCTGGAGGG CGCCGACGTG
    AGCTACGCCA ACCACCGCGG CCGCAGCCCG CTGGACCTGG CCGCCGAGGG CCGTGTACTC
    AAGGCCCTGC AGGGCTGTGC GCAGCGCTTC CGGGAGAGGC ACGCGGGCGG CAGCGGGGGT
    GCGGCCCCGG GCCCGAGGCT GGTGCTTGGC ACCCCCAACA CCGTGACGAA CCTGCACGTG
    GCTGCGCCGT CGGGGCCCGA GGCCGCCGAG TGCCTGGTGT GCTCGGAGCT GGCGCTGCTG
    GTGCTGTTCT CGCCGTGCCA GCACCGCACT GTGTGCGAGG AGTGCGCTCG CAGGATGAAG
    AAGTGCATCA GATGCCAGGT GGTCATCGGC AAGAAGCTGC GACCAGACGG CACAGAGGTG
    GCCAGCGCGA CCCCCGCGTC CGGCCCGCCG CGGCAGCTGG TGGAGGAGCT GCAGAGTCGC
    TACCGGCAGA TGGAGGAGCG CATCACCTGC CCCATCTGCA TCGACAGCCA CATCCGCCTC
    GTGTTCCAGT GCGGCCACGG CGCGTGCGCA CCCTGCGGCG CCGCGCTCAG CGCCTGCCCC
    ATCTGCCGCC AGCCCATCCG CGACCGCATC CAGATCTTCG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe36587
    Clone ID Related Accession (Same CDS sequence) XM_014113816.2 , XM_014113816.1
    Accession Version XM_014113816.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2865bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726058.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGCAGTG CCCCTTCCCT GCCTATCCTG TGCTGGACAT GGGTCTTGGT GTGGACCTGG 
    GCAGACGGTG GATGTCAGAG GGCCCGTGGG GCTCGGCCCC TGCTCCTGGC AAAACCTGGC
    CACTGCCCCT CCTCACTGCT CACCTGTGGC CTCAGCTGGC TGGCAGTGTT TCCTGAGGGA
    GCCAGGCCTG TGATATGGTC CCGGAGCAGT CCTGCCCACC ATGGACCCAG ACCCCCAGGC
    AGGTGTGCAG GCGTCCGCCA CCCCAACATC ATCTGCGACT GCTGCAAGAA GCACGGCCTG
    CGAGGCATGC GCTGGAAGTG CCGCGTCTGC TTCGACTACG ACCTGTGCAC GCAGTGCTAC
    ATGCACAACA AGCATGACCT CACCCACGCC TTCGAGCGCT ACGAGACAGC CCACTCCCGC
    CCGGTCACGC TGAGTCCCCG CCAGGGCCTC CCGAGGATCC CATTGAGGGG CATCTTCCAG
    GGGGCGAAGG TGGTGCGGGG CCCTGACTGG GAGTGGGGTT CGCAGGATGG AGGGGAAGGG
    AAGCTGGGCC GAGTGGTGGA CATCCGTGGC TGGGATGTGG AGACGGGCCG GAGTGTGGCC
    AGTGTGACGT GGGCTGATGG CACCACCAAT GTGTACCGTG TGGGTCACAA GGGCAAGGTG
    GACCTCAAGT GTGTGAACGA GGCAGCTGGT GGCTTCTACT ACAAGGAGCA CCTCCCGAAG
    CTTGGTAAGC CAGCAGAGCT GCAGCGCAGG GTGAGTGCCG ACAGCCAGCC TTTCCAGCAC
    GGGGACAAGG TTAAGTGTCT GCTGGACACA GACATCCTGA GGGAGATGCA GGAAGGCCAC
    GGCGGGTGGA ACCCCAGGAT GGCGGAGACG GGCACCGTGC ACCGCATCAC GGACCGTGGG
    GATGTGCGTG TGCAGTTCGG CCGTGAGACC CGCTGGACCT TCCACCCTGG GGCTCTCACC
    AAGCACAATG CCTTCTGGGT GGGCGATGTG GTACGGGTCA TCGATGACCT TGACACAGTG
    AAGCGGCTGC AGGCTGGGCA TGGCGAGTGG ACAGATGACA TGGCCCCCGC CCTGGGCCGC
    ATTGGGAAGG TGGTGAAAGT TTTCAGAGAC GGTAACCTGC GTGTGGCAGT CGGTGGTCAG
    CTGTGGACCT TCAGCCCCTC CTGCCTGGTG GCCTACCGGC CTGAGGAGGA TGCCAACCTG
    GATGTGGCCG AACGTGCCAG GGAGAACAAA AGCTTCCTGA GTGTTGCTCT GGAGAAGCTT
    CGAGCCCAGA AGAGTGACCT GGAGCACCCA GGGAGACTGG TGGTGGAGGT GGCCTTGGGC
    AGTATGGCTG GGGCTCTGGA CCAGCTGAGG CGGCACCCAG AGCAGGTGGA CACCAAGAAC
    CAGGGCAGGA CCGCTCTGCA GGTGGCCGCC TACCTAGGCC AGGTGGAGCT GGTGCGGCTG
    CTGCTGCAGG CACGAGCGGG CATGGACCTG GCAGATGATG AGGGCAATAC GGCGCTGCAC
    TATGCAGCCT TGGGGAACCA GCCTGAGGCT GCCCGGTTGC TCCTGAGCTC CGGGTGTGGG
    GCCAATGCTC TTAACAGCAC CCGGAGCGCA GCACTGCATG TGGCTGTGCA GAGGGGCTTC
    CTGGAGGTGG TGAAGGTCCT CTGTGAACGT GGCTGTGACG TCAACCTGCC TGATGCACAC
    GCGGACACAC CTCTGCACTG TGCCATCTCG GCGGGCGCCG GCGCCAGCGG CATCGTGGAG
    GTCCTCACTG AGGTGCCAGG TGTTGATGTC ACGGCCACTA ACAGCCAGGG CTTCACCCTG
    CTGCATCACG CATCCCTCAA GGGCCACACG CTAGCTGTCA GGAGGATTCT AGCTCGGGCA
    CGACAGCTGG TGGACGCCAA GAAGGAGGAT GGCTTCACAG CCTTACACCT GGCTGCCCTC
    AACAACCACC GGGAAGTGGC CCAGATTCTG ATCCGAGAGG GCCGCTGTGA TGTGAACGTT
    CGCAACCGTA AGCTCCAGTC CCCCCTGCAC CTGGCCGTGC AGCAGGCTCA TGTGGGGCTG
    GTGCCGCTGC TAGTGGACGC GGGCTGCAGT GTCAACGCCG AGGATGAGGA AGGGGACACA
    GCCTTGCACG TGGCCCTGCA GCGTCATCAG CTGCTGCCCC TGGCAGCAGA TGGGGCCGGG
    GGGGACCCAG GGCCCTTGCA GCTGCTGTCC AGGCTCCAGG CCTCAGGCCT CCCGGGCAGC
    GGGGAGCTGA CGGTGGGAGC GGCGGTCGCA TGCTTCCTGG CGCTGGAGGG CGCCGACGTG
    AGCTACGCCA ACCACCGCGG CCGCAGCCCG CTGGACCTGG CCGCCGAGGG CCGTGTACTC
    AAGGCCCTGC AGGGCTGTGC GCAGCGCTTC CGGGAGAGGC ACGCGGGCGG CAGCGGGGGT
    GCGGCCCCGG GCCCGAGGCT GGTGCTTGGC ACCCCCAACA CCGTGACGAA CCTGCACGTG
    GCTGCGCCGT CGGGGCCCGA GGCCGCCGAG TGCCTGGTGT GCTCGGAGCT GGCGCTGCTG
    GTGCTGTTCT CGCCGTGCCA GCACCGCACT GTGTGCGAGG AGTGCGCTCG CAGGATGAAG
    AAGTGCATCA GATGCCAGGT GGTCATCGGC AAGAAGCTGC GACCAGACGG CACAGAGGTG
    GCCAGCGCGA CCCCCGCGTC CGGCCCGCCG CGGCAGCTGG TGGAGGAGCT GCAGAGTCGC
    TACCGGCAGA TGGAGGAGCG CATCACCTGC CCCATCTGCA TCGACAGCCA CATCCGCCTC
    GTGTTCCAGT GCGGCCACGG CGCGTGCGCA CCCTGCGGCG CCGCGCTCAG CGCCTGCCCC
    ATCTGCCGCC AGCCCATCCG CGACCGCATC CAGATCTTCG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013969291.1
    CDS1898..4762
    Translation

    Target ORF information:

    RefSeq Version XM_014113816.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014113816.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGGCAGTG CCCCTTCCCT GCCTATCCTG TGCTGGACAT GGGTCTTGGT GTGGACCTGG 
    GCAGACGGTG GATGTCAGAG GGCCCGTGGG GCTCGGCCCC TGCTCCTGGC AAAACCTGGC
    CACTGCCCCT CCTCACTGCT CACCTGTGGC CTCAGCTGGC TGGCAGTGTT TCCTGAGGGA
    GCCAGGCCTG TGATATGGTC CCGGAGCAGT CCTGCCCACC ATGGACCCAG ACCCCCAGGC
    AGGTGTGCAG GCGTCCGCCA CCCCAACATC ATCTGCGACT GCTGCAAGAA GCACGGCCTG
    CGAGGCATGC GCTGGAAGTG CCGCGTCTGC TTCGACTACG ACCTGTGCAC GCAGTGCTAC
    ATGCACAACA AGCATGACCT CACCCACGCC TTCGAGCGCT ACGAGACAGC CCACTCCCGC
    CCGGTCACGC TGAGTCCCCG CCAGGGCCTC CCGAGGATCC CATTGAGGGG CATCTTCCAG
    GGGGCGAAGG TGGTGCGGGG CCCTGACTGG GAGTGGGGTT CGCAGGATGG AGGGGAAGGG
    AAGCTGGGCC GAGTGGTGGA CATCCGTGGC TGGGATGTGG AGACGGGCCG GAGTGTGGCC
    AGTGTGACGT GGGCTGATGG CACCACCAAT GTGTACCGTG TGGGTCACAA GGGCAAGGTG
    GACCTCAAGT GTGTGAACGA GGCAGCTGGT GGCTTCTACT ACAAGGAGCA CCTCCCGAAG
    CTTGGTAAGC CAGCAGAGCT GCAGCGCAGG GTGAGTGCCG ACAGCCAGCC TTTCCAGCAC
    GGGGACAAGG TTAAGTGTCT GCTGGACACA GACATCCTGA GGGAGATGCA GGAAGGCCAC
    GGCGGGTGGA ACCCCAGGAT GGCGGAGACG GGCACCGTGC ACCGCATCAC GGACCGTGGG
    GATGTGCGTG TGCAGTTCGG CCGTGAGACC CGCTGGACCT TCCACCCTGG GGCTCTCACC
    AAGCACAATG CCTTCTGGGT GGGCGATGTG GTACGGGTCA TCGATGACCT TGACACAGTG
    AAGCGGCTGC AGGCTGGGCA TGGCGAGTGG ACAGATGACA TGGCCCCCGC CCTGGGCCGC
    ATTGGGAAGG TGGTGAAAGT TTTCAGAGAC GGTAACCTGC GTGTGGCAGT CGGTGGTCAG
    CTGTGGACCT TCAGCCCCTC CTGCCTGGTG GCCTACCGGC CTGAGGAGGA TGCCAACCTG
    GATGTGGCCG AACGTGCCAG GGAGAACAAA AGCTTCCTGA GTGTTGCTCT GGAGAAGCTT
    CGAGCCCAGA AGAGTGACCT GGAGCACCCA GGGAGACTGG TGGTGGAGGT GGCCTTGGGC
    AGTATGGCTG GGGCTCTGGA CCAGCTGAGG CGGCACCCAG AGCAGGTGGA CACCAAGAAC
    CAGGGCAGGA CCGCTCTGCA GGTGGCCGCC TACCTAGGCC AGGTGGAGCT GGTGCGGCTG
    CTGCTGCAGG CACGAGCGGG CATGGACCTG GCAGATGATG AGGGCAATAC GGCGCTGCAC
    TATGCAGCCT TGGGGAACCA GCCTGAGGCT GCCCGGTTGC TCCTGAGCTC CGGGTGTGGG
    GCCAATGCTC TTAACAGCAC CCGGAGCGCA GCACTGCATG TGGCTGTGCA GAGGGGCTTC
    CTGGAGGTGG TGAAGGTCCT CTGTGAACGT GGCTGTGACG TCAACCTGCC TGATGCACAC
    GCGGACACAC CTCTGCACTG TGCCATCTCG GCGGGCGCCG GCGCCAGCGG CATCGTGGAG
    GTCCTCACTG AGGTGCCAGG TGTTGATGTC ACGGCCACTA ACAGCCAGGG CTTCACCCTG
    CTGCATCACG CATCCCTCAA GGGCCACACG CTAGCTGTCA GGAGGATTCT AGCTCGGGCA
    CGACAGCTGG TGGACGCCAA GAAGGAGGAT GGCTTCACAG CCTTACACCT GGCTGCCCTC
    AACAACCACC GGGAAGTGGC CCAGATTCTG ATCCGAGAGG GCCGCTGTGA TGTGAACGTT
    CGCAACCGTA AGCTCCAGTC CCCCCTGCAC CTGGCCGTGC AGCAGGCTCA TGTGGGGCTG
    GTGCCGCTGC TAGTGGACGC GGGCTGCAGT GTCAACGCCG AGGATGAGGA AGGGGACACA
    GCCTTGCACG TGGCCCTGCA GCGTCATCAG CTGCTGCCCC TGGCAGCAGA TGGGGCCGGG
    GGGGACCCAG GGCCCTTGCA GCTGCTGTCC AGGCTCCAGG CCTCAGGCCT CCCGGGCAGC
    GGGGAGCTGA CGGTGGGAGC GGCGGTCGCA TGCTTCCTGG CGCTGGAGGG CGCCGACGTG
    AGCTACGCCA ACCACCGCGG CCGCAGCCCG CTGGACCTGG CCGCCGAGGG CCGTGTACTC
    AAGGCCCTGC AGGGCTGTGC GCAGCGCTTC CGGGAGAGGC ACGCGGGCGG CAGCGGGGGT
    GCGGCCCCGG GCCCGAGGCT GGTGCTTGGC ACCCCCAACA CCGTGACGAA CCTGCACGTG
    GCTGCGCCGT CGGGGCCCGA GGCCGCCGAG TGCCTGGTGT GCTCGGAGCT GGCGCTGCTG
    GTGCTGTTCT CGCCGTGCCA GCACCGCACT GTGTGCGAGG AGTGCGCTCG CAGGATGAAG
    AAGTGCATCA GATGCCAGGT GGTCATCGGC AAGAAGCTGC GACCAGACGG CACAGAGGTG
    GCCAGCGCGA CCCCCGCGTC CGGCCCGCCG CGGCAGCTGG TGGAGGAGCT GCAGAGTCGC
    TACCGGCAGA TGGAGGAGCG CATCACCTGC CCCATCTGCA TCGACAGCCA CATCCGCCTC
    GTGTTCCAGT GCGGCCACGG CGCGTGCGCA CCCTGCGGCG CCGCGCTCAG CGCCTGCCCC
    ATCTGCCGCC AGCCCATCCG CGACCGCATC CAGATCTTCG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.