mib2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	mib2
Entrez Gene ID	35170
Full Name	mind bomb 2
Synonyms	Be,CG17492,D-mibl,Dmel\CG17492,Mib-2,i115,l(2)37Be,mib1/2,suelto
General protein information	Preferred Names mind bomb 2 Names CG17492 gene product from transcript CG17492-RA CG17492-PA CG17492-PB CG17492-PC lethal (2) 37Be mib2-PA mib2-PB mib2-PC mindbomb2
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 37B10-37B10\|2-53.9 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001273625.1	NP_001260554.1	mind bomb 2, isoform B
NM_001273626.1	NP_001260555.1	mind bomb 2, isoform C
NM_136089.3	NP_609933.1	mind bomb 2, isoform A

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mib2	NP_001005901.2
Xenopus tropicalis (tropical clawed frog)	mib2	NP_001116916.1
Homo sapiens (human)	MIB2	NP_543151.2
Bos taurus (cattle)	MIB2	XP_002694171.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008851	XP_319593.4
Gallus gallus (chicken)	MIB2	NP_001006301.1
Drosophila melanogaster (fruit fly)	mib2	NP_001260554.1
Pan troglodytes (chimpanzee)	MIB2	XP_001155032.3
Mus musculus (house mouse)	Mib2	NP_001243036.1
	MIB2	XP_001096926.2
Canis lupus familiaris (dog)	MIB2	XP_005620446.1
Danio rerio (zebrafish)	mib2	NP_001073146.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mib2 plays an additional important role in the development of adult thorax muscles, particularly in maintaining the larval templates for the dorsal longitudinal indirect flight muscles during metamorphosis.
Title: Identification of the essential protein domains for Mib2 function during the development of the Drosophila larval musculature and adult flight muscles.

Mib2 acts sequentially in myoblast fusion and sarcomeric stability by two separable processes involving distinct functions of Mib2.
Title: Mind bomb 2, a founder myoblast-specific protein, regulates myoblast fusion and muscle stability.

These data suggest strongly that mib2 is involved in a novel Notch- and integrin-independent pathway that maintains the integrity of fully differentiated muscles and prevents their apoptotic degeneration.
Title: Drosophila mind bomb2 is required for maintaining muscle integrity and survival.

The following mib2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mib2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa14931	NM_001273626.1 Latest version!	Drosophila melanogaster mind bomb 2 (mib2), transcript variant C, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa14931	NM_136089.3 Latest version!	Drosophila melanogaster mind bomb 2 (mib2), transcript variant A, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OFa14931	NM_001273625.1 Latest version!	Drosophila melanogaster mind bomb 2 (mib2), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mib2 ( NM_001273625.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260554.1 Drosophila melanogaster mind bomb 2 (mib2), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa14931
Clone ID Related Accession (Same CDS sequence)	NM_001273625.1 , NM_001273626.1 , NM_136089.3
Accession Version	NM_001273625.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mind bomb 2, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGAGCCT CCATTCCGCC CGGCATTCGA GTGGTGCGCG GACCCAACTG GATCTGGTCG 
AATCAAGACG ACGGAGAAGG TCACGTTGGA ACCGTTTGCG AAATCGGACG TTGCGGATCG 
ACACATTCGC CAGAAAACAC TGTCGTGGTC AACTGGGATT CCGGACACCG GACCAACTAC 
CGTGTGGGCT ACCAAAACCA GTACGACTTG ATAATTGTAG ATAATGCTCA AGTTGGAGTT 
CGTCACTCAA ATGTTGTCTG CGATGGCTGT TCCAAGGCGG GTATCGCTGG CATAGTATTC 
AAGTGCGCCC AGTGTCCCAA CTATCACCTA TGCGCCTATT GCTACGCAGA GGATCTGCAC 
GATATTGAGC ACCCGTTTAT CCGCTACACG ACTCCCAACT CGCTGGGCGT GAGATTGCCC 
ATGAGAAAAG GTGCCAAGCG CATCCAGCTG CGAGGCATCT TTGTGGGTTC CAAAGTAGTG 
CGCGGTCCTG ACTGGGAGTG GAACGAACAG GACGGCGGAG AAGGCAGGAC GGGTCGGGTA 
ATGGAAATCC GAGGCTGGGA CAACGAATCG TGTCGCAGCG TGGCCAACGT AGCGTGGGTA 
ACCGGATCGA CGAACGTCTA CCGTCTAGGT CACAAGGGAA ACGTTGACCT CAAGTACATT 
ACTGCCACGT GCGGTGGTCA CTACTATAAG GACCACATGC CTGTTCTAGG TCAGACAGAG 
GAGTTGCAGC CGGTGGCGCC CATGGTGAAG CCAAGCTTTT CCGTGGGCGA CCGCGTCAAG 
GTTTGCCTAG AGGTTGACGC CCTAATGAAA TTGCAGCAGG GCCATGGCGG ATGGAATCCG 
CGCATGGTCG AGCATTTGTC CAAACTGGGC ACAGTACACC GCATCACGGA CAAGGGGGAT 
ATACGTGTTC AGTATGAAAA CTGTCCCAAT CGATGGACCT TTCATCCCGC CGCACTCGTT 
AAGGTTGTCT CTTTTCGTGT CGGTGACCTG GTCACCATCA TCAACGATGC CAACAAAGTG 
CAGCAGCTGC AGAAGGGTCA CGGCGAATGG ATAGAAATCA TGCGACACGC CCTTGGAAAA 
ATTTGCAAGG TAGTGAAGGT GTACTCGGAC GGTGACCTCC GCATCCAGCA GTTGGACGAC 
GGCTTCGAGT GGACCCTGAA TCCGAAATGT GTCAAACTAG AGCGTTCTCC TTTGGCAACA 
GCAGCTGAGC GCTCTAACAG TATGATGGAC CTAAGCCACC GACGCGCTGA CCATGTAATG 
ACACCGCTCT CTGGCCTCTC CGGTAGTTCG GTTGCCGACA AGCTGGTGCG CGAGGCTGCC 
CAGGGCCATT TGGATTTCGT GCGGCAGTAC CTGGACGTGA ATCCCAGCCA GGTGGATGTG 
ATGAGTGGCG GCAAGGCTTG TATTCAGGTG GCCTCTCATC AGGGCTACGT CGATCTTGTC 
AGCTACCTCA TCTCCAAGGG TGCCAACGTG AATGCGGTCG ACAAGGAAGG TGATTCAGCT 
CTGCACTACG CAGCCTTCGG CAATCAGCCG GCCACCATGA GAGTGCTGCT GCAGCACGGT 
GCTGAGGTGA ACTTCCTAAA CTCGAGCCAC TGCTCTGCAC TGCACATCTG CGCGCACAAG 
AAAACGCCGC ACTGTGTGCG TGAGTTGCTG CAGCACAATG CCAACGTAAA CATTCAGGAT 
TCGTACGGGG ATACGGCGCT TCACGACGCA ATCGGCAAAG AGAATACGGA AGTTGTGGAG 
TTGCTTTGTA ATGCTCCGAA TCTGGACTTT ACGGTTAAGA ATAATCGCGG CTTTAACGTG 
CTACACCATG CAGCGCTTAA AGGAAACGTG GTAGCTGCTC GCCGTATCCT GTTGCTCTCC 
CGCCAGCTGG TCAATGTGCG CAAAGACGAT GGCTTTGCGG CTCTTCATTT GGCAGCCCTT 
AACGGTCATG CCCAGGTTGT GGAAACGCTG GTCACTGAGG GCCAAGCGGA GTTAGATATA 
CGCAATAATC GCCAACAGAC GCCGTTCCTA CTGGCCGTCT CCCAGGGCCA TGCCGGGGTA 
ATCGAGCGAC TCGTTCGCCT CTCCTGCGAC GTGAATGCCA AGGATGAGGA TGGCGACAAT 
GCGATGCACT TGTGCGTCAT TAAGAAGTCC AATCTGCAGT CGGCCGCGGA GCCTCAGCCA 
GAGGAGGCTC CTGAGATTCA CAAGTTTTAC CTGAGTCTAG TACACTCGAG CATTCGTCCT 
GAGGATCGTT TGATGTACAG TATATTGATC TATCTTTCGA GGGCCGGCTG CCGAGTGGAG 
CTGAACAACG CCAATGCAAG CATTTTTGAG TGGATAACGG ACCGCCACAT CAGGCAACTG 
ATTTTTGGGC AGCAGGGCGA AGCTGAATCG CTGCCTAGGA ATCTTCAAGC TTTGGAAGTG 
TCCGCAGGAT CAGCCGACGG TGAGGAGAGC TCTGCGTCTG CTGGAGCTGA ATCCAATGGA 
GCCGGACCTG GAAGCGTTGC AGCACCCCCA CCACCGCAGC GCCAGACATT TGAACTAATG 
CCAAAGCCAA ACGATATCCC AGCCATTGGA GTTGGAGCAT CTCCATCCAC GCCCTCCGCC 
TCGCCGGGCG TAAAGAAACT GAACTCCGAC GCAACACCGC AAAACGTCTC TCCACAGGTG 
GCACCGCGCA AGAAGGCGCC CAAGCCTCCG GTTACCACTA GCTCGAGCAG TACCGCGTTG 
GAAGCCGGAG CGGCGGGACC CAGTACCAGT CCGGTGATCG TCCCCGGTCC CCATGAGTGC 
ATTGTGTGCA ATGAGATCCT GCCGATGGTG CGCTTTGAGC CGTGTCAGCA TCAGATCGCC 
TGCGAGGAGT GCGGCATCCG AATGAAAAAG TGTCTCCGCT GTGCCGTGGC GATCGAGCGG 
CGATTGACGG TCAGCGGCCG AGTGGTGGCC CTGCCCACGT CCACGTCGTC ACCTAGTGAC 
CCCACTCGCC TACCGTCCGG GGATCTACTT CGCTATCTGG AGAACAAAGT GCTGGAGTTT 
GAGGAGTCGC ACTTCTGCGG TATTTGCATG GAGCGAAAAA GGGATGTGGC CTTCCTGTGC 
GGGCACGGCG CGTGCTCCCA TTGTGCCGAA ACGCTCCGCA CATGCCACAT GTGTCGCAAG 
ACAATACTCA AGAAGATCAA CCTGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260554.1
CDS	399..3548
Translation	MRASIPPGIRVVRGPNWIWSNQDDGEGHVGTVCEIGRCGSTHSPENTVVVNWDSGHRTNYRVGYQNQYDLIIVDNAQVGVRHSNVVCDGCSKAGIAGIVFKCAQCPNYHLCAYCYAEDLHDIEHPFIRYTTPNSLGVRLPMRKGAKRIQLRGIFVGSKVVRGPDWEWNEQDGGEGRTGRVMEIRGWDNESCRSVANVAWVTGSTNVYRLGHKGNVDLKYITATCGGHYYKDHMPVLGQTEELQPVAPMVKPSFSVGDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVKVVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVKLERSPLATAAERSNSMMDLSHRRADHVMTPLSGLSGSSVADKLVREAAQGHLDFVRQYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVRELLQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCDVNAKDEDGDNAMHLCVIKKSNLQSAAEPQPEEAPEIHKFYLSLVHSSIRPEDRLMYSILIYLSRAGCRVELNNANASIFEWITDRHIRQLIFGQQGEAESLPRNLQALEVSAGSADGEESSASAGAESNGAGPGSVAAPPPPQRQTFELMPKPNDIPAIGVGASPSTPSASPGVKKLNSDATPQNVSPQVAPRKKAPKPPVTTSSSSTALEAGAAGPSTSPVIVPGPHECIVCNEILPMVRFEPCQHQIACEECGIRMKKCLRCAVAIERRLTVSGRVVALPTSTSSPSDPTRLPSGDLLRYLENKVLEFEESHFCGICMERKRDVAFLCGHGACSHCAETLRTCHMCRKTILKKINLY

Target ORF information:

RefSeq Version	NM_001273625.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mind bomb 2 (mib2), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273625.1

ORF Insert Sequence:

ATGCGAGCCT CCATTCCGCC CGGCATTCGA GTGGTGCGCG GACCCAACTG GATCTGGTCG 
AATCAAGACG ACGGAGAAGG TCACGTTGGA ACCGTTTGCG AAATCGGACG TTGCGGATCG 
ACACATTCGC CAGAAAACAC TGTCGTGGTC AACTGGGATT CCGGACACCG GACCAACTAC 
CGTGTGGGCT ACCAAAACCA GTACGACTTG ATAATTGTAG ATAATGCTCA AGTTGGAGTT 
CGTCACTCAA ATGTTGTCTG CGATGGCTGT TCCAAGGCGG GTATCGCTGG CATAGTATTC 
AAGTGCGCCC AGTGTCCCAA CTATCACCTA TGCGCCTATT GCTACGCAGA GGATCTGCAC 
GATATTGAGC ACCCGTTTAT CCGCTACACG ACTCCCAACT CGCTGGGCGT GAGATTGCCC 
ATGAGAAAAG GTGCCAAGCG CATCCAGCTG CGAGGCATCT TTGTGGGTTC CAAAGTAGTG 
CGCGGTCCTG ACTGGGAGTG GAACGAACAG GACGGCGGAG AAGGCAGGAC GGGTCGGGTA 
ATGGAAATCC GAGGCTGGGA CAACGAATCG TGTCGCAGCG TGGCCAACGT AGCGTGGGTA 
ACCGGATCGA CGAACGTCTA CCGTCTAGGT CACAAGGGAA ACGTTGACCT CAAGTACATT 
ACTGCCACGT GCGGTGGTCA CTACTATAAG GACCACATGC CTGTTCTAGG TCAGACAGAG 
GAGTTGCAGC CGGTGGCGCC CATGGTGAAG CCAAGCTTTT CCGTGGGCGA CCGCGTCAAG 
GTTTGCCTAG AGGTTGACGC CCTAATGAAA TTGCAGCAGG GCCATGGCGG ATGGAATCCG 
CGCATGGTCG AGCATTTGTC CAAACTGGGC ACAGTACACC GCATCACGGA CAAGGGGGAT 
ATACGTGTTC AGTATGAAAA CTGTCCCAAT CGATGGACCT TTCATCCCGC CGCACTCGTT 
AAGGTTGTCT CTTTTCGTGT CGGTGACCTG GTCACCATCA TCAACGATGC CAACAAAGTG 
CAGCAGCTGC AGAAGGGTCA CGGCGAATGG ATAGAAATCA TGCGACACGC CCTTGGAAAA 
ATTTGCAAGG TAGTGAAGGT GTACTCGGAC GGTGACCTCC GCATCCAGCA GTTGGACGAC 
GGCTTCGAGT GGACCCTGAA TCCGAAATGT GTCAAACTAG AGCGTTCTCC TTTGGCAACA 
GCAGCTGAGC GCTCTAACAG TATGATGGAC CTAAGCCACC GACGCGCTGA CCATGTAATG 
ACACCGCTCT CTGGCCTCTC CGGTAGTTCG GTTGCCGACA AGCTGGTGCG CGAGGCTGCC 
CAGGGCCATT TGGATTTCGT GCGGCAGTAC CTGGACGTGA ATCCCAGCCA GGTGGATGTG 
ATGAGTGGCG GCAAGGCTTG TATTCAGGTG GCCTCTCATC AGGGCTACGT CGATCTTGTC 
AGCTACCTCA TCTCCAAGGG TGCCAACGTG AATGCGGTCG ACAAGGAAGG TGATTCAGCT 
CTGCACTACG CAGCCTTCGG CAATCAGCCG GCCACCATGA GAGTGCTGCT GCAGCACGGT 
GCTGAGGTGA ACTTCCTAAA CTCGAGCCAC TGCTCTGCAC TGCACATCTG CGCGCACAAG 
AAAACGCCGC ACTGTGTGCG TGAGTTGCTG CAGCACAATG CCAACGTAAA CATTCAGGAT 
TCGTACGGGG ATACGGCGCT TCACGACGCA ATCGGCAAAG AGAATACGGA AGTTGTGGAG 
TTGCTTTGTA ATGCTCCGAA TCTGGACTTT ACGGTTAAGA ATAATCGCGG CTTTAACGTG 
CTACACCATG CAGCGCTTAA AGGAAACGTG GTAGCTGCTC GCCGTATCCT GTTGCTCTCC 
CGCCAGCTGG TCAATGTGCG CAAAGACGAT GGCTTTGCGG CTCTTCATTT GGCAGCCCTT 
AACGGTCATG CCCAGGTTGT GGAAACGCTG GTCACTGAGG GCCAAGCGGA GTTAGATATA 
CGCAATAATC GCCAACAGAC GCCGTTCCTA CTGGCCGTCT CCCAGGGCCA TGCCGGGGTA 
ATCGAGCGAC TCGTTCGCCT CTCCTGCGAC GTGAATGCCA AGGATGAGGA TGGCGACAAT 
GCGATGCACT TGTGCGTCAT TAAGAAGTCC AATCTGCAGT CGGCCGCGGA GCCTCAGCCA 
GAGGAGGCTC CTGAGATTCA CAAGTTTTAC CTGAGTCTAG TACACTCGAG CATTCGTCCT 
GAGGATCGTT TGATGTACAG TATATTGATC TATCTTTCGA GGGCCGGCTG CCGAGTGGAG 
CTGAACAACG CCAATGCAAG CATTTTTGAG TGGATAACGG ACCGCCACAT CAGGCAACTG 
ATTTTTGGGC AGCAGGGCGA AGCTGAATCG CTGCCTAGGA ATCTTCAAGC TTTGGAAGTG 
TCCGCAGGAT CAGCCGACGG TGAGGAGAGC TCTGCGTCTG CTGGAGCTGA ATCCAATGGA 
GCCGGACCTG GAAGCGTTGC AGCACCCCCA CCACCGCAGC GCCAGACATT TGAACTAATG 
CCAAAGCCAA ACGATATCCC AGCCATTGGA GTTGGAGCAT CTCCATCCAC GCCCTCCGCC 
TCGCCGGGCG TAAAGAAACT GAACTCCGAC GCAACACCGC AAAACGTCTC TCCACAGGTG 
GCACCGCGCA AGAAGGCGCC CAAGCCTCCG GTTACCACTA GCTCGAGCAG TACCGCGTTG 
GAAGCCGGAG CGGCGGGACC CAGTACCAGT CCGGTGATCG TCCCCGGTCC CCATGAGTGC 
ATTGTGTGCA ATGAGATCCT GCCGATGGTG CGCTTTGAGC CGTGTCAGCA TCAGATCGCC 
TGCGAGGAGT GCGGCATCCG AATGAAAAAG TGTCTCCGCT GTGCCGTGGC GATCGAGCGG 
CGATTGACGG TCAGCGGCCG AGTGGTGGCC CTGCCCACGT CCACGTCGTC ACCTAGTGAC 
CCCACTCGCC TACCGTCCGG GGATCTACTT CGCTATCTGG AGAACAAAGT GCTGGAGTTT 
GAGGAGTCGC ACTTCTGCGG TATTTGCATG GAGCGAAAAA GGGATGTGGC CTTCCTGTGC 
GGGCACGGCG CGTGCTCCCA TTGTGCCGAA ACGCTCCGCA CATGCCACAT GTGTCGCAAG 
ACAATACTCA AGAAGATCAA CCTGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib2 ( NM_001273626.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001260555.1 Drosophila melanogaster mind bomb 2 (mib2), transcript variant C, mRNA.

Price & Availability↑

CloneID	OFa14931
Clone ID Related Accession (Same CDS sequence)	NM_001273625.1 , NM_001273626.1 , NM_136089.3
Accession Version	NM_001273626.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mind bomb 2, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGAGCCT CCATTCCGCC CGGCATTCGA GTGGTGCGCG GACCCAACTG GATCTGGTCG 
AATCAAGACG ACGGAGAAGG TCACGTTGGA ACCGTTTGCG AAATCGGACG TTGCGGATCG 
ACACATTCGC CAGAAAACAC TGTCGTGGTC AACTGGGATT CCGGACACCG GACCAACTAC 
CGTGTGGGCT ACCAAAACCA GTACGACTTG ATAATTGTAG ATAATGCTCA AGTTGGAGTT 
CGTCACTCAA ATGTTGTCTG CGATGGCTGT TCCAAGGCGG GTATCGCTGG CATAGTATTC 
AAGTGCGCCC AGTGTCCCAA CTATCACCTA TGCGCCTATT GCTACGCAGA GGATCTGCAC 
GATATTGAGC ACCCGTTTAT CCGCTACACG ACTCCCAACT CGCTGGGCGT GAGATTGCCC 
ATGAGAAAAG GTGCCAAGCG CATCCAGCTG CGAGGCATCT TTGTGGGTTC CAAAGTAGTG 
CGCGGTCCTG ACTGGGAGTG GAACGAACAG GACGGCGGAG AAGGCAGGAC GGGTCGGGTA 
ATGGAAATCC GAGGCTGGGA CAACGAATCG TGTCGCAGCG TGGCCAACGT AGCGTGGGTA 
ACCGGATCGA CGAACGTCTA CCGTCTAGGT CACAAGGGAA ACGTTGACCT CAAGTACATT 
ACTGCCACGT GCGGTGGTCA CTACTATAAG GACCACATGC CTGTTCTAGG TCAGACAGAG 
GAGTTGCAGC CGGTGGCGCC CATGGTGAAG CCAAGCTTTT CCGTGGGCGA CCGCGTCAAG 
GTTTGCCTAG AGGTTGACGC CCTAATGAAA TTGCAGCAGG GCCATGGCGG ATGGAATCCG 
CGCATGGTCG AGCATTTGTC CAAACTGGGC ACAGTACACC GCATCACGGA CAAGGGGGAT 
ATACGTGTTC AGTATGAAAA CTGTCCCAAT CGATGGACCT TTCATCCCGC CGCACTCGTT 
AAGGTTGTCT CTTTTCGTGT CGGTGACCTG GTCACCATCA TCAACGATGC CAACAAAGTG 
CAGCAGCTGC AGAAGGGTCA CGGCGAATGG ATAGAAATCA TGCGACACGC CCTTGGAAAA 
ATTTGCAAGG TAGTGAAGGT GTACTCGGAC GGTGACCTCC GCATCCAGCA GTTGGACGAC 
GGCTTCGAGT GGACCCTGAA TCCGAAATGT GTCAAACTAG AGCGTTCTCC TTTGGCAACA 
GCAGCTGAGC GCTCTAACAG TATGATGGAC CTAAGCCACC GACGCGCTGA CCATGTAATG 
ACACCGCTCT CTGGCCTCTC CGGTAGTTCG GTTGCCGACA AGCTGGTGCG CGAGGCTGCC 
CAGGGCCATT TGGATTTCGT GCGGCAGTAC CTGGACGTGA ATCCCAGCCA GGTGGATGTG 
ATGAGTGGCG GCAAGGCTTG TATTCAGGTG GCCTCTCATC AGGGCTACGT CGATCTTGTC 
AGCTACCTCA TCTCCAAGGG TGCCAACGTG AATGCGGTCG ACAAGGAAGG TGATTCAGCT 
CTGCACTACG CAGCCTTCGG CAATCAGCCG GCCACCATGA GAGTGCTGCT GCAGCACGGT 
GCTGAGGTGA ACTTCCTAAA CTCGAGCCAC TGCTCTGCAC TGCACATCTG CGCGCACAAG 
AAAACGCCGC ACTGTGTGCG TGAGTTGCTG CAGCACAATG CCAACGTAAA CATTCAGGAT 
TCGTACGGGG ATACGGCGCT TCACGACGCA ATCGGCAAAG AGAATACGGA AGTTGTGGAG 
TTGCTTTGTA ATGCTCCGAA TCTGGACTTT ACGGTTAAGA ATAATCGCGG CTTTAACGTG 
CTACACCATG CAGCGCTTAA AGGAAACGTG GTAGCTGCTC GCCGTATCCT GTTGCTCTCC 
CGCCAGCTGG TCAATGTGCG CAAAGACGAT GGCTTTGCGG CTCTTCATTT GGCAGCCCTT 
AACGGTCATG CCCAGGTTGT GGAAACGCTG GTCACTGAGG GCCAAGCGGA GTTAGATATA 
CGCAATAATC GCCAACAGAC GCCGTTCCTA CTGGCCGTCT CCCAGGGCCA TGCCGGGGTA 
ATCGAGCGAC TCGTTCGCCT CTCCTGCGAC GTGAATGCCA AGGATGAGGA TGGCGACAAT 
GCGATGCACT TGTGCGTCAT TAAGAAGTCC AATCTGCAGT CGGCCGCGGA GCCTCAGCCA 
GAGGAGGCTC CTGAGATTCA CAAGTTTTAC CTGAGTCTAG TACACTCGAG CATTCGTCCT 
GAGGATCGTT TGATGTACAG TATATTGATC TATCTTTCGA GGGCCGGCTG CCGAGTGGAG 
CTGAACAACG CCAATGCAAG CATTTTTGAG TGGATAACGG ACCGCCACAT CAGGCAACTG 
ATTTTTGGGC AGCAGGGCGA AGCTGAATCG CTGCCTAGGA ATCTTCAAGC TTTGGAAGTG 
TCCGCAGGAT CAGCCGACGG TGAGGAGAGC TCTGCGTCTG CTGGAGCTGA ATCCAATGGA 
GCCGGACCTG GAAGCGTTGC AGCACCCCCA CCACCGCAGC GCCAGACATT TGAACTAATG 
CCAAAGCCAA ACGATATCCC AGCCATTGGA GTTGGAGCAT CTCCATCCAC GCCCTCCGCC 
TCGCCGGGCG TAAAGAAACT GAACTCCGAC GCAACACCGC AAAACGTCTC TCCACAGGTG 
GCACCGCGCA AGAAGGCGCC CAAGCCTCCG GTTACCACTA GCTCGAGCAG TACCGCGTTG 
GAAGCCGGAG CGGCGGGACC CAGTACCAGT CCGGTGATCG TCCCCGGTCC CCATGAGTGC 
ATTGTGTGCA ATGAGATCCT GCCGATGGTG CGCTTTGAGC CGTGTCAGCA TCAGATCGCC 
TGCGAGGAGT GCGGCATCCG AATGAAAAAG TGTCTCCGCT GTGCCGTGGC GATCGAGCGG 
CGATTGACGG TCAGCGGCCG AGTGGTGGCC CTGCCCACGT CCACGTCGTC ACCTAGTGAC 
CCCACTCGCC TACCGTCCGG GGATCTACTT CGCTATCTGG AGAACAAAGT GCTGGAGTTT 
GAGGAGTCGC ACTTCTGCGG TATTTGCATG GAGCGAAAAA GGGATGTGGC CTTCCTGTGC 
GGGCACGGCG CGTGCTCCCA TTGTGCCGAA ACGCTCCGCA CATGCCACAT GTGTCGCAAG 
ACAATACTCA AGAAGATCAA CCTGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001260555.1
CDS	237..3386
Translation	MRASIPPGIRVVRGPNWIWSNQDDGEGHVGTVCEIGRCGSTHSPENTVVVNWDSGHRTNYRVGYQNQYDLIIVDNAQVGVRHSNVVCDGCSKAGIAGIVFKCAQCPNYHLCAYCYAEDLHDIEHPFIRYTTPNSLGVRLPMRKGAKRIQLRGIFVGSKVVRGPDWEWNEQDGGEGRTGRVMEIRGWDNESCRSVANVAWVTGSTNVYRLGHKGNVDLKYITATCGGHYYKDHMPVLGQTEELQPVAPMVKPSFSVGDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVKVVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVKLERSPLATAAERSNSMMDLSHRRADHVMTPLSGLSGSSVADKLVREAAQGHLDFVRQYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVRELLQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCDVNAKDEDGDNAMHLCVIKKSNLQSAAEPQPEEAPEIHKFYLSLVHSSIRPEDRLMYSILIYLSRAGCRVELNNANASIFEWITDRHIRQLIFGQQGEAESLPRNLQALEVSAGSADGEESSASAGAESNGAGPGSVAAPPPPQRQTFELMPKPNDIPAIGVGASPSTPSASPGVKKLNSDATPQNVSPQVAPRKKAPKPPVTTSSSSTALEAGAAGPSTSPVIVPGPHECIVCNEILPMVRFEPCQHQIACEECGIRMKKCLRCAVAIERRLTVSGRVVALPTSTSSPSDPTRLPSGDLLRYLENKVLEFEESHFCGICMERKRDVAFLCGHGACSHCAETLRTCHMCRKTILKKINLY

Target ORF information:

RefSeq Version	NM_001273626.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mind bomb 2 (mib2), transcript variant C, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001273626.1

ORF Insert Sequence:

ATGCGAGCCT CCATTCCGCC CGGCATTCGA GTGGTGCGCG GACCCAACTG GATCTGGTCG 
AATCAAGACG ACGGAGAAGG TCACGTTGGA ACCGTTTGCG AAATCGGACG TTGCGGATCG 
ACACATTCGC CAGAAAACAC TGTCGTGGTC AACTGGGATT CCGGACACCG GACCAACTAC 
CGTGTGGGCT ACCAAAACCA GTACGACTTG ATAATTGTAG ATAATGCTCA AGTTGGAGTT 
CGTCACTCAA ATGTTGTCTG CGATGGCTGT TCCAAGGCGG GTATCGCTGG CATAGTATTC 
AAGTGCGCCC AGTGTCCCAA CTATCACCTA TGCGCCTATT GCTACGCAGA GGATCTGCAC 
GATATTGAGC ACCCGTTTAT CCGCTACACG ACTCCCAACT CGCTGGGCGT GAGATTGCCC 
ATGAGAAAAG GTGCCAAGCG CATCCAGCTG CGAGGCATCT TTGTGGGTTC CAAAGTAGTG 
CGCGGTCCTG ACTGGGAGTG GAACGAACAG GACGGCGGAG AAGGCAGGAC GGGTCGGGTA 
ATGGAAATCC GAGGCTGGGA CAACGAATCG TGTCGCAGCG TGGCCAACGT AGCGTGGGTA 
ACCGGATCGA CGAACGTCTA CCGTCTAGGT CACAAGGGAA ACGTTGACCT CAAGTACATT 
ACTGCCACGT GCGGTGGTCA CTACTATAAG GACCACATGC CTGTTCTAGG TCAGACAGAG 
GAGTTGCAGC CGGTGGCGCC CATGGTGAAG CCAAGCTTTT CCGTGGGCGA CCGCGTCAAG 
GTTTGCCTAG AGGTTGACGC CCTAATGAAA TTGCAGCAGG GCCATGGCGG ATGGAATCCG 
CGCATGGTCG AGCATTTGTC CAAACTGGGC ACAGTACACC GCATCACGGA CAAGGGGGAT 
ATACGTGTTC AGTATGAAAA CTGTCCCAAT CGATGGACCT TTCATCCCGC CGCACTCGTT 
AAGGTTGTCT CTTTTCGTGT CGGTGACCTG GTCACCATCA TCAACGATGC CAACAAAGTG 
CAGCAGCTGC AGAAGGGTCA CGGCGAATGG ATAGAAATCA TGCGACACGC CCTTGGAAAA 
ATTTGCAAGG TAGTGAAGGT GTACTCGGAC GGTGACCTCC GCATCCAGCA GTTGGACGAC 
GGCTTCGAGT GGACCCTGAA TCCGAAATGT GTCAAACTAG AGCGTTCTCC TTTGGCAACA 
GCAGCTGAGC GCTCTAACAG TATGATGGAC CTAAGCCACC GACGCGCTGA CCATGTAATG 
ACACCGCTCT CTGGCCTCTC CGGTAGTTCG GTTGCCGACA AGCTGGTGCG CGAGGCTGCC 
CAGGGCCATT TGGATTTCGT GCGGCAGTAC CTGGACGTGA ATCCCAGCCA GGTGGATGTG 
ATGAGTGGCG GCAAGGCTTG TATTCAGGTG GCCTCTCATC AGGGCTACGT CGATCTTGTC 
AGCTACCTCA TCTCCAAGGG TGCCAACGTG AATGCGGTCG ACAAGGAAGG TGATTCAGCT 
CTGCACTACG CAGCCTTCGG CAATCAGCCG GCCACCATGA GAGTGCTGCT GCAGCACGGT 
GCTGAGGTGA ACTTCCTAAA CTCGAGCCAC TGCTCTGCAC TGCACATCTG CGCGCACAAG 
AAAACGCCGC ACTGTGTGCG TGAGTTGCTG CAGCACAATG CCAACGTAAA CATTCAGGAT 
TCGTACGGGG ATACGGCGCT TCACGACGCA ATCGGCAAAG AGAATACGGA AGTTGTGGAG 
TTGCTTTGTA ATGCTCCGAA TCTGGACTTT ACGGTTAAGA ATAATCGCGG CTTTAACGTG 
CTACACCATG CAGCGCTTAA AGGAAACGTG GTAGCTGCTC GCCGTATCCT GTTGCTCTCC 
CGCCAGCTGG TCAATGTGCG CAAAGACGAT GGCTTTGCGG CTCTTCATTT GGCAGCCCTT 
AACGGTCATG CCCAGGTTGT GGAAACGCTG GTCACTGAGG GCCAAGCGGA GTTAGATATA 
CGCAATAATC GCCAACAGAC GCCGTTCCTA CTGGCCGTCT CCCAGGGCCA TGCCGGGGTA 
ATCGAGCGAC TCGTTCGCCT CTCCTGCGAC GTGAATGCCA AGGATGAGGA TGGCGACAAT 
GCGATGCACT TGTGCGTCAT TAAGAAGTCC AATCTGCAGT CGGCCGCGGA GCCTCAGCCA 
GAGGAGGCTC CTGAGATTCA CAAGTTTTAC CTGAGTCTAG TACACTCGAG CATTCGTCCT 
GAGGATCGTT TGATGTACAG TATATTGATC TATCTTTCGA GGGCCGGCTG CCGAGTGGAG 
CTGAACAACG CCAATGCAAG CATTTTTGAG TGGATAACGG ACCGCCACAT CAGGCAACTG 
ATTTTTGGGC AGCAGGGCGA AGCTGAATCG CTGCCTAGGA ATCTTCAAGC TTTGGAAGTG 
TCCGCAGGAT CAGCCGACGG TGAGGAGAGC TCTGCGTCTG CTGGAGCTGA ATCCAATGGA 
GCCGGACCTG GAAGCGTTGC AGCACCCCCA CCACCGCAGC GCCAGACATT TGAACTAATG 
CCAAAGCCAA ACGATATCCC AGCCATTGGA GTTGGAGCAT CTCCATCCAC GCCCTCCGCC 
TCGCCGGGCG TAAAGAAACT GAACTCCGAC GCAACACCGC AAAACGTCTC TCCACAGGTG 
GCACCGCGCA AGAAGGCGCC CAAGCCTCCG GTTACCACTA GCTCGAGCAG TACCGCGTTG 
GAAGCCGGAG CGGCGGGACC CAGTACCAGT CCGGTGATCG TCCCCGGTCC CCATGAGTGC 
ATTGTGTGCA ATGAGATCCT GCCGATGGTG CGCTTTGAGC CGTGTCAGCA TCAGATCGCC 
TGCGAGGAGT GCGGCATCCG AATGAAAAAG TGTCTCCGCT GTGCCGTGGC GATCGAGCGG 
CGATTGACGG TCAGCGGCCG AGTGGTGGCC CTGCCCACGT CCACGTCGTC ACCTAGTGAC 
CCCACTCGCC TACCGTCCGG GGATCTACTT CGCTATCTGG AGAACAAAGT GCTGGAGTTT 
GAGGAGTCGC ACTTCTGCGG TATTTGCATG GAGCGAAAAA GGGATGTGGC CTTCCTGTGC 
GGGCACGGCG CGTGCTCCCA TTGTGCCGAA ACGCTCCGCA CATGCCACAT GTGTCGCAAG 
ACAATACTCA AGAAGATCAA CCTGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib2 ( NM_136089.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609933.1 Drosophila melanogaster mind bomb 2 (mib2), transcript variant A, mRNA.

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CloneID	OFa14931
Clone ID Related Accession (Same CDS sequence)	NM_001273625.1 , NM_001273626.1 , NM_136089.3
Accession Version	NM_136089.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3150bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	mind bomb 2, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jan 16, 2013 this sequence version replaced NM_136089.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCGAGCCT CCATTCCGCC CGGCATTCGA GTGGTGCGCG GACCCAACTG GATCTGGTCG 
AATCAAGACG ACGGAGAAGG TCACGTTGGA ACCGTTTGCG AAATCGGACG TTGCGGATCG 
ACACATTCGC CAGAAAACAC TGTCGTGGTC AACTGGGATT CCGGACACCG GACCAACTAC 
CGTGTGGGCT ACCAAAACCA GTACGACTTG ATAATTGTAG ATAATGCTCA AGTTGGAGTT 
CGTCACTCAA ATGTTGTCTG CGATGGCTGT TCCAAGGCGG GTATCGCTGG CATAGTATTC 
AAGTGCGCCC AGTGTCCCAA CTATCACCTA TGCGCCTATT GCTACGCAGA GGATCTGCAC 
GATATTGAGC ACCCGTTTAT CCGCTACACG ACTCCCAACT CGCTGGGCGT GAGATTGCCC 
ATGAGAAAAG GTGCCAAGCG CATCCAGCTG CGAGGCATCT TTGTGGGTTC CAAAGTAGTG 
CGCGGTCCTG ACTGGGAGTG GAACGAACAG GACGGCGGAG AAGGCAGGAC GGGTCGGGTA 
ATGGAAATCC GAGGCTGGGA CAACGAATCG TGTCGCAGCG TGGCCAACGT AGCGTGGGTA 
ACCGGATCGA CGAACGTCTA CCGTCTAGGT CACAAGGGAA ACGTTGACCT CAAGTACATT 
ACTGCCACGT GCGGTGGTCA CTACTATAAG GACCACATGC CTGTTCTAGG TCAGACAGAG 
GAGTTGCAGC CGGTGGCGCC CATGGTGAAG CCAAGCTTTT CCGTGGGCGA CCGCGTCAAG 
GTTTGCCTAG AGGTTGACGC CCTAATGAAA TTGCAGCAGG GCCATGGCGG ATGGAATCCG 
CGCATGGTCG AGCATTTGTC CAAACTGGGC ACAGTACACC GCATCACGGA CAAGGGGGAT 
ATACGTGTTC AGTATGAAAA CTGTCCCAAT CGATGGACCT TTCATCCCGC CGCACTCGTT 
AAGGTTGTCT CTTTTCGTGT CGGTGACCTG GTCACCATCA TCAACGATGC CAACAAAGTG 
CAGCAGCTGC AGAAGGGTCA CGGCGAATGG ATAGAAATCA TGCGACACGC CCTTGGAAAA 
ATTTGCAAGG TAGTGAAGGT GTACTCGGAC GGTGACCTCC GCATCCAGCA GTTGGACGAC 
GGCTTCGAGT GGACCCTGAA TCCGAAATGT GTCAAACTAG AGCGTTCTCC TTTGGCAACA 
GCAGCTGAGC GCTCTAACAG TATGATGGAC CTAAGCCACC GACGCGCTGA CCATGTAATG 
ACACCGCTCT CTGGCCTCTC CGGTAGTTCG GTTGCCGACA AGCTGGTGCG CGAGGCTGCC 
CAGGGCCATT TGGATTTCGT GCGGCAGTAC CTGGACGTGA ATCCCAGCCA GGTGGATGTG 
ATGAGTGGCG GCAAGGCTTG TATTCAGGTG GCCTCTCATC AGGGCTACGT CGATCTTGTC 
AGCTACCTCA TCTCCAAGGG TGCCAACGTG AATGCGGTCG ACAAGGAAGG TGATTCAGCT 
CTGCACTACG CAGCCTTCGG CAATCAGCCG GCCACCATGA GAGTGCTGCT GCAGCACGGT 
GCTGAGGTGA ACTTCCTAAA CTCGAGCCAC TGCTCTGCAC TGCACATCTG CGCGCACAAG 
AAAACGCCGC ACTGTGTGCG TGAGTTGCTG CAGCACAATG CCAACGTAAA CATTCAGGAT 
TCGTACGGGG ATACGGCGCT TCACGACGCA ATCGGCAAAG AGAATACGGA AGTTGTGGAG 
TTGCTTTGTA ATGCTCCGAA TCTGGACTTT ACGGTTAAGA ATAATCGCGG CTTTAACGTG 
CTACACCATG CAGCGCTTAA AGGAAACGTG GTAGCTGCTC GCCGTATCCT GTTGCTCTCC 
CGCCAGCTGG TCAATGTGCG CAAAGACGAT GGCTTTGCGG CTCTTCATTT GGCAGCCCTT 
AACGGTCATG CCCAGGTTGT GGAAACGCTG GTCACTGAGG GCCAAGCGGA GTTAGATATA 
CGCAATAATC GCCAACAGAC GCCGTTCCTA CTGGCCGTCT CCCAGGGCCA TGCCGGGGTA 
ATCGAGCGAC TCGTTCGCCT CTCCTGCGAC GTGAATGCCA AGGATGAGGA TGGCGACAAT 
GCGATGCACT TGTGCGTCAT TAAGAAGTCC AATCTGCAGT CGGCCGCGGA GCCTCAGCCA 
GAGGAGGCTC CTGAGATTCA CAAGTTTTAC CTGAGTCTAG TACACTCGAG CATTCGTCCT 
GAGGATCGTT TGATGTACAG TATATTGATC TATCTTTCGA GGGCCGGCTG CCGAGTGGAG 
CTGAACAACG CCAATGCAAG CATTTTTGAG TGGATAACGG ACCGCCACAT CAGGCAACTG 
ATTTTTGGGC AGCAGGGCGA AGCTGAATCG CTGCCTAGGA ATCTTCAAGC TTTGGAAGTG 
TCCGCAGGAT CAGCCGACGG TGAGGAGAGC TCTGCGTCTG CTGGAGCTGA ATCCAATGGA 
GCCGGACCTG GAAGCGTTGC AGCACCCCCA CCACCGCAGC GCCAGACATT TGAACTAATG 
CCAAAGCCAA ACGATATCCC AGCCATTGGA GTTGGAGCAT CTCCATCCAC GCCCTCCGCC 
TCGCCGGGCG TAAAGAAACT GAACTCCGAC GCAACACCGC AAAACGTCTC TCCACAGGTG 
GCACCGCGCA AGAAGGCGCC CAAGCCTCCG GTTACCACTA GCTCGAGCAG TACCGCGTTG 
GAAGCCGGAG CGGCGGGACC CAGTACCAGT CCGGTGATCG TCCCCGGTCC CCATGAGTGC 
ATTGTGTGCA ATGAGATCCT GCCGATGGTG CGCTTTGAGC CGTGTCAGCA TCAGATCGCC 
TGCGAGGAGT GCGGCATCCG AATGAAAAAG TGTCTCCGCT GTGCCGTGGC GATCGAGCGG 
CGATTGACGG TCAGCGGCCG AGTGGTGGCC CTGCCCACGT CCACGTCGTC ACCTAGTGAC 
CCCACTCGCC TACCGTCCGG GGATCTACTT CGCTATCTGG AGAACAAAGT GCTGGAGTTT 
GAGGAGTCGC ACTTCTGCGG TATTTGCATG GAGCGAAAAA GGGATGTGGC CTTCCTGTGC 
GGGCACGGCG CGTGCTCCCA TTGTGCCGAA ACGCTCCGCA CATGCCACAT GTGTCGCAAG 
ACAATACTCA AGAAGATCAA CCTGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609933.1
CDS	596..3745
Translation	MRASIPPGIRVVRGPNWIWSNQDDGEGHVGTVCEIGRCGSTHSPENTVVVNWDSGHRTNYRVGYQNQYDLIIVDNAQVGVRHSNVVCDGCSKAGIAGIVFKCAQCPNYHLCAYCYAEDLHDIEHPFIRYTTPNSLGVRLPMRKGAKRIQLRGIFVGSKVVRGPDWEWNEQDGGEGRTGRVMEIRGWDNESCRSVANVAWVTGSTNVYRLGHKGNVDLKYITATCGGHYYKDHMPVLGQTEELQPVAPMVKPSFSVGDRVKVCLEVDALMKLQQGHGGWNPRMVEHLSKLGTVHRITDKGDIRVQYENCPNRWTFHPAALVKVVSFRVGDLVTIINDANKVQQLQKGHGEWIEIMRHALGKICKVVKVYSDGDLRIQQLDDGFEWTLNPKCVKLERSPLATAAERSNSMMDLSHRRADHVMTPLSGLSGSSVADKLVREAAQGHLDFVRQYLDVNPSQVDVMSGGKACIQVASHQGYVDLVSYLISKGANVNAVDKEGDSALHYAAFGNQPATMRVLLQHGAEVNFLNSSHCSALHICAHKKTPHCVRELLQHNANVNIQDSYGDTALHDAIGKENTEVVELLCNAPNLDFTVKNNRGFNVLHHAALKGNVVAARRILLLSRQLVNVRKDDGFAALHLAALNGHAQVVETLVTEGQAELDIRNNRQQTPFLLAVSQGHAGVIERLVRLSCDVNAKDEDGDNAMHLCVIKKSNLQSAAEPQPEEAPEIHKFYLSLVHSSIRPEDRLMYSILIYLSRAGCRVELNNANASIFEWITDRHIRQLIFGQQGEAESLPRNLQALEVSAGSADGEESSASAGAESNGAGPGSVAAPPPPQRQTFELMPKPNDIPAIGVGASPSTPSASPGVKKLNSDATPQNVSPQVAPRKKAPKPPVTTSSSSTALEAGAAGPSTSPVIVPGPHECIVCNEILPMVRFEPCQHQIACEECGIRMKKCLRCAVAIERRLTVSGRVVALPTSTSSPSDPTRLPSGDLLRYLENKVLEFEESHFCGICMERKRDVAFLCGHGACSHCAETLRTCHMCRKTILKKINLY

Target ORF information:

RefSeq Version	NM_136089.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster mind bomb 2 (mib2), transcript variant A, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_136089.3

ORF Insert Sequence:

ATGCGAGCCT CCATTCCGCC CGGCATTCGA GTGGTGCGCG GACCCAACTG GATCTGGTCG 
AATCAAGACG ACGGAGAAGG TCACGTTGGA ACCGTTTGCG AAATCGGACG TTGCGGATCG 
ACACATTCGC CAGAAAACAC TGTCGTGGTC AACTGGGATT CCGGACACCG GACCAACTAC 
CGTGTGGGCT ACCAAAACCA GTACGACTTG ATAATTGTAG ATAATGCTCA AGTTGGAGTT 
CGTCACTCAA ATGTTGTCTG CGATGGCTGT TCCAAGGCGG GTATCGCTGG CATAGTATTC 
AAGTGCGCCC AGTGTCCCAA CTATCACCTA TGCGCCTATT GCTACGCAGA GGATCTGCAC 
GATATTGAGC ACCCGTTTAT CCGCTACACG ACTCCCAACT CGCTGGGCGT GAGATTGCCC 
ATGAGAAAAG GTGCCAAGCG CATCCAGCTG CGAGGCATCT TTGTGGGTTC CAAAGTAGTG 
CGCGGTCCTG ACTGGGAGTG GAACGAACAG GACGGCGGAG AAGGCAGGAC GGGTCGGGTA 
ATGGAAATCC GAGGCTGGGA CAACGAATCG TGTCGCAGCG TGGCCAACGT AGCGTGGGTA 
ACCGGATCGA CGAACGTCTA CCGTCTAGGT CACAAGGGAA ACGTTGACCT CAAGTACATT 
ACTGCCACGT GCGGTGGTCA CTACTATAAG GACCACATGC CTGTTCTAGG TCAGACAGAG 
GAGTTGCAGC CGGTGGCGCC CATGGTGAAG CCAAGCTTTT CCGTGGGCGA CCGCGTCAAG 
GTTTGCCTAG AGGTTGACGC CCTAATGAAA TTGCAGCAGG GCCATGGCGG ATGGAATCCG 
CGCATGGTCG AGCATTTGTC CAAACTGGGC ACAGTACACC GCATCACGGA CAAGGGGGAT 
ATACGTGTTC AGTATGAAAA CTGTCCCAAT CGATGGACCT TTCATCCCGC CGCACTCGTT 
AAGGTTGTCT CTTTTCGTGT CGGTGACCTG GTCACCATCA TCAACGATGC CAACAAAGTG 
CAGCAGCTGC AGAAGGGTCA CGGCGAATGG ATAGAAATCA TGCGACACGC CCTTGGAAAA 
ATTTGCAAGG TAGTGAAGGT GTACTCGGAC GGTGACCTCC GCATCCAGCA GTTGGACGAC 
GGCTTCGAGT GGACCCTGAA TCCGAAATGT GTCAAACTAG AGCGTTCTCC TTTGGCAACA 
GCAGCTGAGC GCTCTAACAG TATGATGGAC CTAAGCCACC GACGCGCTGA CCATGTAATG 
ACACCGCTCT CTGGCCTCTC CGGTAGTTCG GTTGCCGACA AGCTGGTGCG CGAGGCTGCC 
CAGGGCCATT TGGATTTCGT GCGGCAGTAC CTGGACGTGA ATCCCAGCCA GGTGGATGTG 
ATGAGTGGCG GCAAGGCTTG TATTCAGGTG GCCTCTCATC AGGGCTACGT CGATCTTGTC 
AGCTACCTCA TCTCCAAGGG TGCCAACGTG AATGCGGTCG ACAAGGAAGG TGATTCAGCT 
CTGCACTACG CAGCCTTCGG CAATCAGCCG GCCACCATGA GAGTGCTGCT GCAGCACGGT 
GCTGAGGTGA ACTTCCTAAA CTCGAGCCAC TGCTCTGCAC TGCACATCTG CGCGCACAAG 
AAAACGCCGC ACTGTGTGCG TGAGTTGCTG CAGCACAATG CCAACGTAAA CATTCAGGAT 
TCGTACGGGG ATACGGCGCT TCACGACGCA ATCGGCAAAG AGAATACGGA AGTTGTGGAG 
TTGCTTTGTA ATGCTCCGAA TCTGGACTTT ACGGTTAAGA ATAATCGCGG CTTTAACGTG 
CTACACCATG CAGCGCTTAA AGGAAACGTG GTAGCTGCTC GCCGTATCCT GTTGCTCTCC 
CGCCAGCTGG TCAATGTGCG CAAAGACGAT GGCTTTGCGG CTCTTCATTT GGCAGCCCTT 
AACGGTCATG CCCAGGTTGT GGAAACGCTG GTCACTGAGG GCCAAGCGGA GTTAGATATA 
CGCAATAATC GCCAACAGAC GCCGTTCCTA CTGGCCGTCT CCCAGGGCCA TGCCGGGGTA 
ATCGAGCGAC TCGTTCGCCT CTCCTGCGAC GTGAATGCCA AGGATGAGGA TGGCGACAAT 
GCGATGCACT TGTGCGTCAT TAAGAAGTCC AATCTGCAGT CGGCCGCGGA GCCTCAGCCA 
GAGGAGGCTC CTGAGATTCA CAAGTTTTAC CTGAGTCTAG TACACTCGAG CATTCGTCCT 
GAGGATCGTT TGATGTACAG TATATTGATC TATCTTTCGA GGGCCGGCTG CCGAGTGGAG 
CTGAACAACG CCAATGCAAG CATTTTTGAG TGGATAACGG ACCGCCACAT CAGGCAACTG 
ATTTTTGGGC AGCAGGGCGA AGCTGAATCG CTGCCTAGGA ATCTTCAAGC TTTGGAAGTG 
TCCGCAGGAT CAGCCGACGG TGAGGAGAGC TCTGCGTCTG CTGGAGCTGA ATCCAATGGA 
GCCGGACCTG GAAGCGTTGC AGCACCCCCA CCACCGCAGC GCCAGACATT TGAACTAATG 
CCAAAGCCAA ACGATATCCC AGCCATTGGA GTTGGAGCAT CTCCATCCAC GCCCTCCGCC 
TCGCCGGGCG TAAAGAAACT GAACTCCGAC GCAACACCGC AAAACGTCTC TCCACAGGTG 
GCACCGCGCA AGAAGGCGCC CAAGCCTCCG GTTACCACTA GCTCGAGCAG TACCGCGTTG 
GAAGCCGGAG CGGCGGGACC CAGTACCAGT CCGGTGATCG TCCCCGGTCC CCATGAGTGC 
ATTGTGTGCA ATGAGATCCT GCCGATGGTG CGCTTTGAGC CGTGTCAGCA TCAGATCGCC 
TGCGAGGAGT GCGGCATCCG AATGAAAAAG TGTCTCCGCT GTGCCGTGGC GATCGAGCGG 
CGATTGACGG TCAGCGGCCG AGTGGTGGCC CTGCCCACGT CCACGTCGTC ACCTAGTGAC 
CCCACTCGCC TACCGTCCGG GGATCTACTT CGCTATCTGG AGAACAAAGT GCTGGAGTTT 
GAGGAGTCGC ACTTCTGCGG TATTTGCATG GAGCGAAAAA GGGATGTGGC CTTCCTGTGC 
GGGCACGGCG CGTGCTCCCA TTGTGCCGAA ACGCTCCGCA CATGCCACAT GTGTCGCAAG 
ACAATACTCA AGAAGATCAA CCTGTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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mib2 cDNA ORF clone, Drosophila melanogaster(fruit fly)

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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