Mib2 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mib2
Entrez Gene ID	76580
Full Name	mindbomb E3 ubiquitin protein ligase 2
Synonyms	2210008I11Rik,Zzank1,skd
General protein information	Preferred Names mindbomb E3 ubiquitin protein ligase 2 Names E3 ubiquitin-protein ligase MIB2 RING-type E3 ubiquitin transferase MIB2 dyslike dystrophin-like protein mind bomb-2 mindbomb homolog 2 skeletrophin zinc finger, ZZ type with ankyrin repeat domain 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 4 Map Location: 4\|4 E2

mRNA and Protein(s)

mRNA	Protein	Name
XM_006539250.2	XP_006539313.1	E3 ubiquitin-protein ligase MIB2 isoform X2
XM_006539252.2	XP_006539315.1	E3 ubiquitin-protein ligase MIB2 isoform X3
NM_001369168.1	NP_001356097.1	E3 ubiquitin-protein ligase MIB2 isoform 6
NM_001369166.1	NP_001356095.1	E3 ubiquitin-protein ligase MIB2 isoform 4
NM_001369167.1	NP_001356096.1	E3 ubiquitin-protein ligase MIB2 isoform 5
NM_001369163.1	NP_001356092.1	E3 ubiquitin-protein ligase MIB2 isoform 2
XM_006539251.3	XP_006539314.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_006539254.3	XP_006539317.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_030253883.1	XP_030109743.1	E3 ubiquitin-protein ligase MIB2 isoform X6
XM_006539249.3	XP_006539312.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_030253879.1	XP_030109739.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_006539249.4	XP_006539312.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_006539253.2	XP_006539316.1	E3 ubiquitin-protein ligase MIB2 isoform X3
XM_011250353.2	XP_011248655.1	E3 ubiquitin-protein ligase MIB2 isoform X7
NM_001256108.2	NP_001243037.2	E3 ubiquitin-protein ligase MIB2 isoform 2
NM_001369164.1	NP_001356093.1	E3 ubiquitin-protein ligase MIB2 isoform 3
NM_001369161.1	NP_001356090.1	E3 ubiquitin-protein ligase MIB2 isoform 1
XM_006539251.2	XP_006539314.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_017320438.1	XP_017175927.1	E3 ubiquitin-protein ligase MIB2 isoform X7
XM_006539247.2	XP_006539310.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_030253881.1	XP_030109741.1	E3 ubiquitin-protein ligase MIB2 isoform X3
XM_030253882.1	XP_030109742.1	E3 ubiquitin-protein ligase MIB2 isoform X3
XM_006539255.2	XP_006539318.1	E3 ubiquitin-protein ligase MIB2 isoform X4
NM_001369165.1	NP_001356094.1	E3 ubiquitin-protein ligase MIB2 isoform 3
NM_001256107.1	NP_001243036.1	E3 ubiquitin-protein ligase MIB2 isoform 1
XM_030253880.1	XP_030109740.1	E3 ubiquitin-protein ligase MIB2 isoform X2
XM_017320437.1	XP_017175926.1	E3 ubiquitin-protein ligase MIB2 isoform X5
NM_145124.3	NP_660106.2	E3 ubiquitin-protein ligase MIB2 isoform 2
NM_001369162.1	NP_001356091.1	E3 ubiquitin-protein ligase MIB2 isoform 2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mib2	NP_001005901.2
Xenopus tropicalis (tropical clawed frog)	mib2	NP_001116916.1
Homo sapiens (human)	MIB2	NP_543151.2
Bos taurus (cattle)	MIB2	XP_002694171.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008851	XP_319593.4
Gallus gallus (chicken)	MIB2	NP_001006301.1
Drosophila melanogaster (fruit fly)	mib2	NP_001260554.1
Pan troglodytes (chimpanzee)	MIB2	XP_001155032.3
Mus musculus (house mouse)	Mib2	NP_001243036.1
	MIB2	XP_001096926.2
Canis lupus familiaris (dog)	MIB2	XP_005620446.1
Danio rerio (zebrafish)	mib2	NP_001073146.1

Gene Ontology
Bibliography

Related articles in PubMed

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Thompson CL, Ng L, Menon V, Martinez S, Lee CK, Glattfelder K, Sunkin SM, Henry A, Lau C, Dang C, Garcia-Lopez R, Martinez-Ferre A, Pombero A, Rubenstein JLR, Wakeman WB, Hohmann J, Dee N, Sodt AJ, Young R, Smith K, Nguyen TN, Kidney J, Kuan L, Jeromin A, Kaykas A, Miller J, Page D, Orta G, Bernard A, Riley Z, Smith S, Wohnoutka P, Hawrylycz MJ, Puelles L, Jones AR
Neuron83(2)309-323(2014 Jul)

The E3 ubiquitin ligase mind bomb-2 (MIB2) protein controls B-cell CLL/lymphoma 10 (BCL10)-dependent NF-?B activation.
Stempin CC, Chi L, Giraldo-Vela JP, High AA, H?cker H, Redecke V
The Journal of biological chemistry286(43)37147-57(2011 Oct)

Mapping a dynamic innate immunity protein interaction network regulating type I interferon production.
Li S, Wang L, Berman M, Kong YY, Dorf ME
Immunity35(3)426-40(2011 Sep)

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Yokoyama S, Ito Y, Ueno-Kudoh H, Shimizu H, Uchibe K, Albini S, Mitsuoka K, Miyaki S, Kiso M, Nagai A, Hikata T, Osada T, Fukuda N, Yamashita S, Harada D, Mezzano V, Kasai M, Puri PL, Hayashizaki Y, Okado H, Hashimoto M, Asahara H
Developmental cell17(6)836-48(2009 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

identify MIB2 as a novel component of the activated BCL10 signaling complex and a missing link in the BCL10-dependent NF-kappaB signaling pathway.
Title: The E3 ubiquitin ligase mind bomb-2 (MIB2) protein controls B-cell CLL/lymphoma 10 (BCL10)-dependent NF-?B activation.

created a targeted mutation of Mib2 and show that its phenotype is a variable penetrance, failure to close the cranial neural tube
Title: Targeted disruption of Mib2 causes exencephaly with a variable penetrance.

Mib2 has functional similarities to Mib1, but might have distinct roles in Notch signaling as an E3 ubiquitin ligase
Title: Mind bomb-2 is an E3 ligase for Notch ligand.

The following Mib2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mib2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu06022	NM_001256107.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu06022	NM_001369161.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu06024	NM_001369163.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu06024	NM_001369162.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu06024	NM_001256108.2 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu06024	NM_145124.3 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu34473	NM_001369164.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu34473	NM_001369165.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu34472	XM_006539250.2 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu34473	XM_006539252.2 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu34474	XM_006539253.2 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu34475	XM_006539255.2 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu71497	XM_011250353.2 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OMu84716	NM_001369168.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu84717	NM_001369166.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu84718	NM_001369167.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu34471	XM_006539251.3 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu34471	XM_006539249.4 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu34471	XM_006539247.2 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu34471	XM_030253879.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu34471	XM_006539254.3 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu34472	XM_030253880.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu34474	XM_030253882.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu34474	XM_030253881.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OMu81120	XM_017320437.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu81121	XM_017320438.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OMu88645	XM_030253883.1 Latest version!	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OMu34471	XM_006539249.3	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu34471	XM_006539251.2	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mib2 ( NM_001369161.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001356090.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OMu06022
Clone ID Related Accession (Same CDS sequence)	NM_001369161.1 , NM_001256107.1
Accession Version	NM_001369161.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627405.17. Transcript Variant: This variant (4), as well as variant 1, encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.3401345.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGATGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAACCA CGAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG 
CACAACAGCT TCTGGGTGGG TGATGTGGTC CGGGTCATCG GTGACCTTGA CACTGTGAAG 
CGACTTCAGG CCGGACATGG TGAATGGACC GACGACATGG CTCCCGCCCT GGGCCGAGTG 
GGGAAAGTGG TGAAGGTGTT TGGAGATGGA AACTTGCGTG TGGCGGTTGG CGGTCAGCGG 
TGGACCTTCA GCCCCTCTTG CCTAGTGGCC TACCGGCCTG AGGAAGACGC CAACCTGGAT 
GTGGCTGAGC GTGCCAGAGA GAACAAAAGC TCACTGAGTG TGGCCCTGGA CAAGCTTCGG 
ACCCAGAAGA GTGACCCAGA GCACCCGGGG AGGCTGGTAG TAGAGGCCGC ACTAGGAAAT 
GTAGCCCGGG CTCTAGATCT TCTGCGGAGG CATCCAGAGC AGGTGGACAC CAAGAACCAG 
GGTAGAACTG CCCTGCAGGT GGCTGCTTAC CTGGGCCAGG TAGAGCTGGT GCGGCTGCTG 
CTGCAGGCAA GAGCAAGTAT GGACCTGCCG GATGATGAGG GCAACACTGT GCTGCACTAC 
ACAGCCATGG GGAACCAGCC TGAGGCCACA AGGGTGCTCC TGAGTGCAGG GTGTGCAGTG 
GATGCTCGGA ATGGCACACG CAGCACAGCC CTACATGTGG CTGTACAGAG GGGCTTCCTA 
GAGGTGGTAA AGATCCTGTG TGAACGTGGT TGTGATGTCA ACTTGCCGGA TGCGCATGCA 
GACACACCCC TGCATTCTGC CATCTCTGCT GGTGCTGGTG CCAGCAGCAT TGTTGAAGTC 
CTCACCGAGG TCCCTGGCAT CGATGTCACT GCTACCAATA GCCAGGGCTT CACACTGCTA 
CACCATGCAT CCCTCAAGGG CCATGTGCTA GCAGTCAGGA AGATTCTGGC AAGGGCCCGG 
CAGCTGGTGG ATGCCAAGAA GGAGGATGGC TTTACCGCAC TGCATCTGGC AGCCCTCAAC 
AACCACCGGG AGGTGGCCCA GGTCCTAATC CGAGAGGGTC GCTGTGATGT GAATGTGCGG 
AACCGGAAGC TTCAGTCTCC ACTTCATCTG GCTGTACAGC AGGCCCACCT GGGGCTGGTA 
CCGCTGCTAG TGGACGCAGG CTGCAGTGTC AACACGGAGG ACGAGGAGGG TGACACGGCC 
CTGCATGTGG CACTACAGCG TCACCAGCTG TTGCCCCTTG TGGCTGACAG GGCTGGGGGA 
GACCCAGGGC CCTTGCAGCT GCTGTCAAGG CTACAGGCCT CAGGCCTCCC AGGCTGCACA 
GAGTTGACTG TAGGCGCTGC AGTGGCCTGC TTCCTGGCGT TGGAGGGTGC TGATGTGAGC 
TACGCAAACC ACCGCGGCCG GAGCCCACTG GACCTGGCCA CAGAGGGCCG AGTGCTCAAG 
GCCTTGCAGG GCTGTGCCCA GCGCTTCAGG GAGCGACAGG CAGGTGGCGG TGGGGGTGTG 
CCCCCGGGCC CCCGGCATGT GCTGAGTACT CCTAACACCG TGACGAACCT GCACGTGTCT 
GGCACGGCAG GGCCAGAAGC TGCCGAGTGC CTGGTGTGCT CGGAGCTGGC ATTGCTAATT 
CTGTTCTCAC CGTGTCAGCA CCGCACAGTG TGTGAGGAGT GCGCTCGAAG GATGAAGAAG 
TGTATCAGGT GCCAGGTGGT CATCAGCAAG AAGCTCCGCC CAGACGGCTC AGAGGTGGTA 
AATGCCATCC AGGTGCCCGG CCCGCCTCGG CAACTGGTGG AGGAGCTACA GAGCCGCTAC 
AGGCAGATGG AGGAGCGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTGGTG 
TTCCAGTGCG GCCATGGAGC GTGCGCGCCC TGTGGCGCCG CGCTTAACGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATTCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356090.1
CDS	741..3602
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_001369161.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369161.1

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGATGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAACCA CGAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG 
CACAACAGCT TCTGGGTGGG TGATGTGGTC CGGGTCATCG GTGACCTTGA CACTGTGAAG 
CGACTTCAGG CCGGACATGG TGAATGGACC GACGACATGG CTCCCGCCCT GGGCCGAGTG 
GGGAAAGTGG TGAAGGTGTT TGGAGATGGA AACTTGCGTG TGGCGGTTGG CGGTCAGCGG 
TGGACCTTCA GCCCCTCTTG CCTAGTGGCC TACCGGCCTG AGGAAGACGC CAACCTGGAT 
GTGGCTGAGC GTGCCAGAGA GAACAAAAGC TCACTGAGTG TGGCCCTGGA CAAGCTTCGG 
ACCCAGAAGA GTGACCCAGA GCACCCGGGG AGGCTGGTAG TAGAGGCCGC ACTAGGAAAT 
GTAGCCCGGG CTCTAGATCT TCTGCGGAGG CATCCAGAGC AGGTGGACAC CAAGAACCAG 
GGTAGAACTG CCCTGCAGGT GGCTGCTTAC CTGGGCCAGG TAGAGCTGGT GCGGCTGCTG 
CTGCAGGCAA GAGCAAGTAT GGACCTGCCG GATGATGAGG GCAACACTGT GCTGCACTAC 
ACAGCCATGG GGAACCAGCC TGAGGCCACA AGGGTGCTCC TGAGTGCAGG GTGTGCAGTG 
GATGCTCGGA ATGGCACACG CAGCACAGCC CTACATGTGG CTGTACAGAG GGGCTTCCTA 
GAGGTGGTAA AGATCCTGTG TGAACGTGGT TGTGATGTCA ACTTGCCGGA TGCGCATGCA 
GACACACCCC TGCATTCTGC CATCTCTGCT GGTGCTGGTG CCAGCAGCAT TGTTGAAGTC 
CTCACCGAGG TCCCTGGCAT CGATGTCACT GCTACCAATA GCCAGGGCTT CACACTGCTA 
CACCATGCAT CCCTCAAGGG CCATGTGCTA GCAGTCAGGA AGATTCTGGC AAGGGCCCGG 
CAGCTGGTGG ATGCCAAGAA GGAGGATGGC TTTACCGCAC TGCATCTGGC AGCCCTCAAC 
AACCACCGGG AGGTGGCCCA GGTCCTAATC CGAGAGGGTC GCTGTGATGT GAATGTGCGG 
AACCGGAAGC TTCAGTCTCC ACTTCATCTG GCTGTACAGC AGGCCCACCT GGGGCTGGTA 
CCGCTGCTAG TGGACGCAGG CTGCAGTGTC AACACGGAGG ACGAGGAGGG TGACACGGCC 
CTGCATGTGG CACTACAGCG TCACCAGCTG TTGCCCCTTG TGGCTGACAG GGCTGGGGGA 
GACCCAGGGC CCTTGCAGCT GCTGTCAAGG CTACAGGCCT CAGGCCTCCC AGGCTGCACA 
GAGTTGACTG TAGGCGCTGC AGTGGCCTGC TTCCTGGCGT TGGAGGGTGC TGATGTGAGC 
TACGCAAACC ACCGCGGCCG GAGCCCACTG GACCTGGCCA CAGAGGGCCG AGTGCTCAAG 
GCCTTGCAGG GCTGTGCCCA GCGCTTCAGG GAGCGACAGG CAGGTGGCGG TGGGGGTGTG 
CCCCCGGGCC CCCGGCATGT GCTGAGTACT CCTAACACCG TGACGAACCT GCACGTGTCT 
GGCACGGCAG GGCCAGAAGC TGCCGAGTGC CTGGTGTGCT CGGAGCTGGC ATTGCTAATT 
CTGTTCTCAC CGTGTCAGCA CCGCACAGTG TGTGAGGAGT GCGCTCGAAG GATGAAGAAG 
TGTATCAGGT GCCAGGTGGT CATCAGCAAG AAGCTCCGCC CAGACGGCTC AGAGGTGGTA 
AATGCCATCC AGGTGCCCGG CCCGCCTCGG CAACTGGTGG AGGAGCTACA GAGCCGCTAC 
AGGCAGATGG AGGAGCGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTGGTG 
TTCCAGTGCG GCCATGGAGC GTGCGCGCCC TGTGGCGCCG CGCTTAACGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATTCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( NM_001256107.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001243036.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu06022
Clone ID Related Accession (Same CDS sequence)	NM_001369161.1 , NM_001256107.1
Accession Version	NM_001256107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2862bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ076202.1, AK147940.1, AK159611.1 and AI837194.1. Transcript Variant: This variant (1), as well as variant 4, encodes isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK159611.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGATGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAACCA CGAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG 
CACAACAGCT TCTGGGTGGG TGATGTGGTC CGGGTCATCG GTGACCTTGA CACTGTGAAG 
CGACTTCAGG CCGGACATGG TGAATGGACC GACGACATGG CTCCCGCCCT GGGCCGAGTG 
GGGAAAGTGG TGAAGGTGTT TGGAGATGGA AACTTGCGTG TGGCGGTTGG CGGTCAGCGG 
TGGACCTTCA GCCCCTCTTG CCTAGTGGCC TACCGGCCTG AGGAAGACGC CAACCTGGAT 
GTGGCTGAGC GTGCCAGAGA GAACAAAAGC TCACTGAGTG TGGCCCTGGA CAAGCTTCGG 
ACCCAGAAGA GTGACCCAGA GCACCCGGGG AGGCTGGTAG TAGAGGCCGC ACTAGGAAAT 
GTAGCCCGGG CTCTAGATCT TCTGCGGAGG CATCCAGAGC AGGTGGACAC CAAGAACCAG 
GGTAGAACTG CCCTGCAGGT GGCTGCTTAC CTGGGCCAGG TAGAGCTGGT GCGGCTGCTG 
CTGCAGGCAA GAGCAAGTAT GGACCTGCCG GATGATGAGG GCAACACTGT GCTGCACTAC 
ACAGCCATGG GGAACCAGCC TGAGGCCACA AGGGTGCTCC TGAGTGCAGG GTGTGCAGTG 
GATGCTCGGA ATGGCACACG CAGCACAGCC CTACATGTGG CTGTACAGAG GGGCTTCCTA 
GAGGTGGTAA AGATCCTGTG TGAACGTGGT TGTGATGTCA ACTTGCCGGA TGCGCATGCA 
GACACACCCC TGCATTCTGC CATCTCTGCT GGTGCTGGTG CCAGCAGCAT TGTTGAAGTC 
CTCACCGAGG TCCCTGGCAT CGATGTCACT GCTACCAATA GCCAGGGCTT CACACTGCTA 
CACCATGCAT CCCTCAAGGG CCATGTGCTA GCAGTCAGGA AGATTCTGGC AAGGGCCCGG 
CAGCTGGTGG ATGCCAAGAA GGAGGATGGC TTTACCGCAC TGCATCTGGC AGCCCTCAAC 
AACCACCGGG AGGTGGCCCA GGTCCTAATC CGAGAGGGTC GCTGTGATGT GAATGTGCGG 
AACCGGAAGC TTCAGTCTCC ACTTCATCTG GCTGTACAGC AGGCCCACCT GGGGCTGGTA 
CCGCTGCTAG TGGACGCAGG CTGCAGTGTC AACACGGAGG ACGAGGAGGG TGACACGGCC 
CTGCATGTGG CACTACAGCG TCACCAGCTG TTGCCCCTTG TGGCTGACAG GGCTGGGGGA 
GACCCAGGGC CCTTGCAGCT GCTGTCAAGG CTACAGGCCT CAGGCCTCCC AGGCTGCACA 
GAGTTGACTG TAGGCGCTGC AGTGGCCTGC TTCCTGGCGT TGGAGGGTGC TGATGTGAGC 
TACGCAAACC ACCGCGGCCG GAGCCCACTG GACCTGGCCA CAGAGGGCCG AGTGCTCAAG 
GCCTTGCAGG GCTGTGCCCA GCGCTTCAGG GAGCGACAGG CAGGTGGCGG TGGGGGTGTG 
CCCCCGGGCC CCCGGCATGT GCTGAGTACT CCTAACACCG TGACGAACCT GCACGTGTCT 
GGCACGGCAG GGCCAGAAGC TGCCGAGTGC CTGGTGTGCT CGGAGCTGGC ATTGCTAATT 
CTGTTCTCAC CGTGTCAGCA CCGCACAGTG TGTGAGGAGT GCGCTCGAAG GATGAAGAAG 
TGTATCAGGT GCCAGGTGGT CATCAGCAAG AAGCTCCGCC CAGACGGCTC AGAGGTGGTA 
AATGCCATCC AGGTGCCCGG CCCGCCTCGG CAACTGGTGG AGGAGCTACA GAGCCGCTAC 
AGGCAGATGG AGGAGCGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTGGTG 
TTCCAGTGCG GCCATGGAGC GTGCGCGCCC TGTGGCGCCG CGCTTAACGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATTCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243036.1
CDS	860..3721
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_001256107.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256107.1

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGATGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAACCA CGAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG 
CACAACAGCT TCTGGGTGGG TGATGTGGTC CGGGTCATCG GTGACCTTGA CACTGTGAAG 
CGACTTCAGG CCGGACATGG TGAATGGACC GACGACATGG CTCCCGCCCT GGGCCGAGTG 
GGGAAAGTGG TGAAGGTGTT TGGAGATGGA AACTTGCGTG TGGCGGTTGG CGGTCAGCGG 
TGGACCTTCA GCCCCTCTTG CCTAGTGGCC TACCGGCCTG AGGAAGACGC CAACCTGGAT 
GTGGCTGAGC GTGCCAGAGA GAACAAAAGC TCACTGAGTG TGGCCCTGGA CAAGCTTCGG 
ACCCAGAAGA GTGACCCAGA GCACCCGGGG AGGCTGGTAG TAGAGGCCGC ACTAGGAAAT 
GTAGCCCGGG CTCTAGATCT TCTGCGGAGG CATCCAGAGC AGGTGGACAC CAAGAACCAG 
GGTAGAACTG CCCTGCAGGT GGCTGCTTAC CTGGGCCAGG TAGAGCTGGT GCGGCTGCTG 
CTGCAGGCAA GAGCAAGTAT GGACCTGCCG GATGATGAGG GCAACACTGT GCTGCACTAC 
ACAGCCATGG GGAACCAGCC TGAGGCCACA AGGGTGCTCC TGAGTGCAGG GTGTGCAGTG 
GATGCTCGGA ATGGCACACG CAGCACAGCC CTACATGTGG CTGTACAGAG GGGCTTCCTA 
GAGGTGGTAA AGATCCTGTG TGAACGTGGT TGTGATGTCA ACTTGCCGGA TGCGCATGCA 
GACACACCCC TGCATTCTGC CATCTCTGCT GGTGCTGGTG CCAGCAGCAT TGTTGAAGTC 
CTCACCGAGG TCCCTGGCAT CGATGTCACT GCTACCAATA GCCAGGGCTT CACACTGCTA 
CACCATGCAT CCCTCAAGGG CCATGTGCTA GCAGTCAGGA AGATTCTGGC AAGGGCCCGG 
CAGCTGGTGG ATGCCAAGAA GGAGGATGGC TTTACCGCAC TGCATCTGGC AGCCCTCAAC 
AACCACCGGG AGGTGGCCCA GGTCCTAATC CGAGAGGGTC GCTGTGATGT GAATGTGCGG 
AACCGGAAGC TTCAGTCTCC ACTTCATCTG GCTGTACAGC AGGCCCACCT GGGGCTGGTA 
CCGCTGCTAG TGGACGCAGG CTGCAGTGTC AACACGGAGG ACGAGGAGGG TGACACGGCC 
CTGCATGTGG CACTACAGCG TCACCAGCTG TTGCCCCTTG TGGCTGACAG GGCTGGGGGA 
GACCCAGGGC CCTTGCAGCT GCTGTCAAGG CTACAGGCCT CAGGCCTCCC AGGCTGCACA 
GAGTTGACTG TAGGCGCTGC AGTGGCCTGC TTCCTGGCGT TGGAGGGTGC TGATGTGAGC 
TACGCAAACC ACCGCGGCCG GAGCCCACTG GACCTGGCCA CAGAGGGCCG AGTGCTCAAG 
GCCTTGCAGG GCTGTGCCCA GCGCTTCAGG GAGCGACAGG CAGGTGGCGG TGGGGGTGTG 
CCCCCGGGCC CCCGGCATGT GCTGAGTACT CCTAACACCG TGACGAACCT GCACGTGTCT 
GGCACGGCAG GGCCAGAAGC TGCCGAGTGC CTGGTGTGCT CGGAGCTGGC ATTGCTAATT 
CTGTTCTCAC CGTGTCAGCA CCGCACAGTG TGTGAGGAGT GCGCTCGAAG GATGAAGAAG 
TGTATCAGGT GCCAGGTGGT CATCAGCAAG AAGCTCCGCC CAGACGGCTC AGAGGTGGTA 
AATGCCATCC AGGTGCCCGG CCCGCCTCGG CAACTGGTGG AGGAGCTACA GAGCCGCTAC 
AGGCAGATGG AGGAGCGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTGGTG 
TTCCAGTGCG GCCATGGAGC GTGCGCGCCC TGTGGCGCCG CGCTTAACGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATTCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( NM_001369163.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001356092.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OMu06024
Clone ID Related Accession (Same CDS sequence)	NM_001369163.1 , NM_001256108.2 , NM_145124.3 , NM_001369162.1
Accession Version	NM_001369163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627405.17. Transcript Variant: This variant (6), as well as variants 2, 3, and 5, encodes isoform 2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.1964239.1, SRR7345562.3514257.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGCTCACTG 
AGTGTGGCCC TGGACAAGCT TCGGACCCAG AAGAGTGACC CAGAGCACCC GGGGAGGCTG 
GTAGTAGAGG CCGCACTAGG AAATGTAGCC CGGGCTCTAG ATCTTCTGCG GAGGCATCCA 
GAGCAGGTGG ACACCAAGAA CCAGGGTAGA ACTGCCCTGC AGGTGGCTGC TTACCTGGGC 
CAGGTAGAGC TGGTGCGGCT GCTGCTGCAG GCAAGAGCAA GTATGGACCT GCCGGATGAT 
GAGGGCAACA CTGTGCTGCA CTACACAGCC ATGGGGAACC AGCCTGAGGC CACAAGGGTG 
CTCCTGAGTG CAGGGTGTGC AGTGGATGCT CGGAATGGCA CACGCAGCAC AGCCCTACAT 
GTGGCTGTAC AGAGGGGCTT CCTAGAGGTG GTAAAGATCC TGTGTGAACG TGGTTGTGAT 
GTCAACTTGC CGGATGCGCA TGCAGACACA CCCCTGCATT CTGCCATCTC TGCTGGTGCT 
GGTGCCAGCA GCATTGTTGA AGTCCTCACC GAGGTCCCTG GCATCGATGT CACTGCTACC 
AATAGCCAGG GCTTCACACT GCTACACCAT GCATCCCTCA AGGGCCATGT GCTAGCAGTC 
AGGAAGATTC TGGCAAGGGC CCGGCAGCTG GTGGATGCCA AGAAGGAGGA TGGCTTTACC 
GCACTGCATC TGGCAGCCCT CAACAACCAC CGGGAGGTGG CCCAGGTCCT AATCCGAGAG 
GGTCGCTGTG ATGTGAATGT GCGGAACCGG AAGCTTCAGT CTCCACTTCA TCTGGCTGTA 
CAGCAGGCCC ACCTGGGGCT GGTACCGCTG CTAGTGGACG CAGGCTGCAG TGTCAACACG 
GAGGACGAGG AGGGTGACAC GGCCCTGCAT GTGGCACTAC AGCGTCACCA GCTGTTGCCC 
CTTGTGGCTG ACAGGGCTGG GGGAGACCCA GGGCCCTTGC AGCTGCTGTC AAGGCTACAG 
GCCTCAGGCC TCCCAGGCTG CACAGAGTTG ACTGTAGGCG CTGCAGTGGC CTGCTTCCTG 
GCGTTGGAGG GTGCTGATGT GAGCTACGCA AACCACCGCG GCCGGAGCCC ACTGGACCTG 
GCCACAGAGG GCCGAGTGCT CAAGGCCTTG CAGGGCTGTG CCCAGCGCTT CAGGGAGCGA 
CAGGCAGGTG GCGGTGGGGG TGTGCCCCCG GGCCCCCGGC ATGTGCTGAG TACTCCTAAC 
ACCGTGACGA ACCTGCACGT GTCTGGCACG GCAGGGCCAG AAGCTGCCGA GTGCCTGGTG 
TGCTCGGAGC TGGCATTGCT AATTCTGTTC TCACCGTGTC AGCACCGCAC AGTGTGTGAG 
GAGTGCGCTC GAAGGATGAA GAAGTGTATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTC 
CGCCCAGACG GCTCAGAGGT GGTAAATGCC ATCCAGGTGC CCGGCCCGCC TCGGCAACTG 
GTGGAGGAGC TACAGAGCCG CTACAGGCAG ATGGAGGAGC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT GGTGTTCCAG TGCGGCCATG GAGCGTGCGC GCCCTGTGGC 
GCCGCGCTTA ACGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT TCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356092.1
CDS	565..3330
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_001369163.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369163.1

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGCTCACTG 
AGTGTGGCCC TGGACAAGCT TCGGACCCAG AAGAGTGACC CAGAGCACCC GGGGAGGCTG 
GTAGTAGAGG CCGCACTAGG AAATGTAGCC CGGGCTCTAG ATCTTCTGCG GAGGCATCCA 
GAGCAGGTGG ACACCAAGAA CCAGGGTAGA ACTGCCCTGC AGGTGGCTGC TTACCTGGGC 
CAGGTAGAGC TGGTGCGGCT GCTGCTGCAG GCAAGAGCAA GTATGGACCT GCCGGATGAT 
GAGGGCAACA CTGTGCTGCA CTACACAGCC ATGGGGAACC AGCCTGAGGC CACAAGGGTG 
CTCCTGAGTG CAGGGTGTGC AGTGGATGCT CGGAATGGCA CACGCAGCAC AGCCCTACAT 
GTGGCTGTAC AGAGGGGCTT CCTAGAGGTG GTAAAGATCC TGTGTGAACG TGGTTGTGAT 
GTCAACTTGC CGGATGCGCA TGCAGACACA CCCCTGCATT CTGCCATCTC TGCTGGTGCT 
GGTGCCAGCA GCATTGTTGA AGTCCTCACC GAGGTCCCTG GCATCGATGT CACTGCTACC 
AATAGCCAGG GCTTCACACT GCTACACCAT GCATCCCTCA AGGGCCATGT GCTAGCAGTC 
AGGAAGATTC TGGCAAGGGC CCGGCAGCTG GTGGATGCCA AGAAGGAGGA TGGCTTTACC 
GCACTGCATC TGGCAGCCCT CAACAACCAC CGGGAGGTGG CCCAGGTCCT AATCCGAGAG 
GGTCGCTGTG ATGTGAATGT GCGGAACCGG AAGCTTCAGT CTCCACTTCA TCTGGCTGTA 
CAGCAGGCCC ACCTGGGGCT GGTACCGCTG CTAGTGGACG CAGGCTGCAG TGTCAACACG 
GAGGACGAGG AGGGTGACAC GGCCCTGCAT GTGGCACTAC AGCGTCACCA GCTGTTGCCC 
CTTGTGGCTG ACAGGGCTGG GGGAGACCCA GGGCCCTTGC AGCTGCTGTC AAGGCTACAG 
GCCTCAGGCC TCCCAGGCTG CACAGAGTTG ACTGTAGGCG CTGCAGTGGC CTGCTTCCTG 
GCGTTGGAGG GTGCTGATGT GAGCTACGCA AACCACCGCG GCCGGAGCCC ACTGGACCTG 
GCCACAGAGG GCCGAGTGCT CAAGGCCTTG CAGGGCTGTG CCCAGCGCTT CAGGGAGCGA 
CAGGCAGGTG GCGGTGGGGG TGTGCCCCCG GGCCCCCGGC ATGTGCTGAG TACTCCTAAC 
ACCGTGACGA ACCTGCACGT GTCTGGCACG GCAGGGCCAG AAGCTGCCGA GTGCCTGGTG 
TGCTCGGAGC TGGCATTGCT AATTCTGTTC TCACCGTGTC AGCACCGCAC AGTGTGTGAG 
GAGTGCGCTC GAAGGATGAA GAAGTGTATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTC 
CGCCCAGACG GCTCAGAGGT GGTAAATGCC ATCCAGGTGC CCGGCCCGCC TCGGCAACTG 
GTGGAGGAGC TACAGAGCCG CTACAGGCAG ATGGAGGAGC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT GGTGTTCCAG TGCGGCCATG GAGCGTGCGC GCCCTGTGGC 
GCCGCGCTTA ACGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT TCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( NM_001256108.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001243037.2 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OMu06024
Clone ID Related Accession (Same CDS sequence)	NM_001369163.1 , NM_001256108.2 , NM_145124.3 , NM_001369162.1
Accession Version	NM_001256108.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ076202.1, AK147940.1, AK159611.1 and AI837194.1. On Aug 22, 2012 this sequence version replaced NM_001256108.1. Transcript Variant: This variant (3) differs in the 5' UTR and lacks an in-frame exon in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Variants 2, 3, 5, and 6 all encode the same isoform (2). ##Evidence-Data-START## CDS exon combination :: AB072336.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGCTCACTG 
AGTGTGGCCC TGGACAAGCT TCGGACCCAG AAGAGTGACC CAGAGCACCC GGGGAGGCTG 
GTAGTAGAGG CCGCACTAGG AAATGTAGCC CGGGCTCTAG ATCTTCTGCG GAGGCATCCA 
GAGCAGGTGG ACACCAAGAA CCAGGGTAGA ACTGCCCTGC AGGTGGCTGC TTACCTGGGC 
CAGGTAGAGC TGGTGCGGCT GCTGCTGCAG GCAAGAGCAA GTATGGACCT GCCGGATGAT 
GAGGGCAACA CTGTGCTGCA CTACACAGCC ATGGGGAACC AGCCTGAGGC CACAAGGGTG 
CTCCTGAGTG CAGGGTGTGC AGTGGATGCT CGGAATGGCA CACGCAGCAC AGCCCTACAT 
GTGGCTGTAC AGAGGGGCTT CCTAGAGGTG GTAAAGATCC TGTGTGAACG TGGTTGTGAT 
GTCAACTTGC CGGATGCGCA TGCAGACACA CCCCTGCATT CTGCCATCTC TGCTGGTGCT 
GGTGCCAGCA GCATTGTTGA AGTCCTCACC GAGGTCCCTG GCATCGATGT CACTGCTACC 
AATAGCCAGG GCTTCACACT GCTACACCAT GCATCCCTCA AGGGCCATGT GCTAGCAGTC 
AGGAAGATTC TGGCAAGGGC CCGGCAGCTG GTGGATGCCA AGAAGGAGGA TGGCTTTACC 
GCACTGCATC TGGCAGCCCT CAACAACCAC CGGGAGGTGG CCCAGGTCCT AATCCGAGAG 
GGTCGCTGTG ATGTGAATGT GCGGAACCGG AAGCTTCAGT CTCCACTTCA TCTGGCTGTA 
CAGCAGGCCC ACCTGGGGCT GGTACCGCTG CTAGTGGACG CAGGCTGCAG TGTCAACACG 
GAGGACGAGG AGGGTGACAC GGCCCTGCAT GTGGCACTAC AGCGTCACCA GCTGTTGCCC 
CTTGTGGCTG ACAGGGCTGG GGGAGACCCA GGGCCCTTGC AGCTGCTGTC AAGGCTACAG 
GCCTCAGGCC TCCCAGGCTG CACAGAGTTG ACTGTAGGCG CTGCAGTGGC CTGCTTCCTG 
GCGTTGGAGG GTGCTGATGT GAGCTACGCA AACCACCGCG GCCGGAGCCC ACTGGACCTG 
GCCACAGAGG GCCGAGTGCT CAAGGCCTTG CAGGGCTGTG CCCAGCGCTT CAGGGAGCGA 
CAGGCAGGTG GCGGTGGGGG TGTGCCCCCG GGCCCCCGGC ATGTGCTGAG TACTCCTAAC 
ACCGTGACGA ACCTGCACGT GTCTGGCACG GCAGGGCCAG AAGCTGCCGA GTGCCTGGTG 
TGCTCGGAGC TGGCATTGCT AATTCTGTTC TCACCGTGTC AGCACCGCAC AGTGTGTGAG 
GAGTGCGCTC GAAGGATGAA GAAGTGTATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTC 
CGCCCAGACG GCTCAGAGGT GGTAAATGCC ATCCAGGTGC CCGGCCCGCC TCGGCAACTG 
GTGGAGGAGC TACAGAGCCG CTACAGGCAG ATGGAGGAGC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT GGTGTTCCAG TGCGGCCATG GAGCGTGCGC GCCCTGTGGC 
GCCGCGCTTA ACGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT TCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243037.2
CDS	776..3541
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_001256108.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256108.2

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGCTCACTG 
AGTGTGGCCC TGGACAAGCT TCGGACCCAG AAGAGTGACC CAGAGCACCC GGGGAGGCTG 
GTAGTAGAGG CCGCACTAGG AAATGTAGCC CGGGCTCTAG ATCTTCTGCG GAGGCATCCA 
GAGCAGGTGG ACACCAAGAA CCAGGGTAGA ACTGCCCTGC AGGTGGCTGC TTACCTGGGC 
CAGGTAGAGC TGGTGCGGCT GCTGCTGCAG GCAAGAGCAA GTATGGACCT GCCGGATGAT 
GAGGGCAACA CTGTGCTGCA CTACACAGCC ATGGGGAACC AGCCTGAGGC CACAAGGGTG 
CTCCTGAGTG CAGGGTGTGC AGTGGATGCT CGGAATGGCA CACGCAGCAC AGCCCTACAT 
GTGGCTGTAC AGAGGGGCTT CCTAGAGGTG GTAAAGATCC TGTGTGAACG TGGTTGTGAT 
GTCAACTTGC CGGATGCGCA TGCAGACACA CCCCTGCATT CTGCCATCTC TGCTGGTGCT 
GGTGCCAGCA GCATTGTTGA AGTCCTCACC GAGGTCCCTG GCATCGATGT CACTGCTACC 
AATAGCCAGG GCTTCACACT GCTACACCAT GCATCCCTCA AGGGCCATGT GCTAGCAGTC 
AGGAAGATTC TGGCAAGGGC CCGGCAGCTG GTGGATGCCA AGAAGGAGGA TGGCTTTACC 
GCACTGCATC TGGCAGCCCT CAACAACCAC CGGGAGGTGG CCCAGGTCCT AATCCGAGAG 
GGTCGCTGTG ATGTGAATGT GCGGAACCGG AAGCTTCAGT CTCCACTTCA TCTGGCTGTA 
CAGCAGGCCC ACCTGGGGCT GGTACCGCTG CTAGTGGACG CAGGCTGCAG TGTCAACACG 
GAGGACGAGG AGGGTGACAC GGCCCTGCAT GTGGCACTAC AGCGTCACCA GCTGTTGCCC 
CTTGTGGCTG ACAGGGCTGG GGGAGACCCA GGGCCCTTGC AGCTGCTGTC AAGGCTACAG 
GCCTCAGGCC TCCCAGGCTG CACAGAGTTG ACTGTAGGCG CTGCAGTGGC CTGCTTCCTG 
GCGTTGGAGG GTGCTGATGT GAGCTACGCA AACCACCGCG GCCGGAGCCC ACTGGACCTG 
GCCACAGAGG GCCGAGTGCT CAAGGCCTTG CAGGGCTGTG CCCAGCGCTT CAGGGAGCGA 
CAGGCAGGTG GCGGTGGGGG TGTGCCCCCG GGCCCCCGGC ATGTGCTGAG TACTCCTAAC 
ACCGTGACGA ACCTGCACGT GTCTGGCACG GCAGGGCCAG AAGCTGCCGA GTGCCTGGTG 
TGCTCGGAGC TGGCATTGCT AATTCTGTTC TCACCGTGTC AGCACCGCAC AGTGTGTGAG 
GAGTGCGCTC GAAGGATGAA GAAGTGTATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTC 
CGCCCAGACG GCTCAGAGGT GGTAAATGCC ATCCAGGTGC CCGGCCCGCC TCGGCAACTG 
GTGGAGGAGC TACAGAGCCG CTACAGGCAG ATGGAGGAGC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT GGTGTTCCAG TGCGGCCATG GAGCGTGCGC GCCCTGTGGC 
GCCGCGCTTA ACGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT TCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( NM_145124.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_660106.2 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu06024
Clone ID Related Accession (Same CDS sequence)	NM_001369163.1 , NM_001256108.2 , NM_145124.3 , NM_001369162.1
Accession Version	NM_145124.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ076202.1, AK147940.1, AK159611.1 and AI837194.1. On Jan 5, 2012 this sequence version replaced NM_145124.2. Transcript Variant: This variant (2) lacks an in-frame exon in the 5' coding region compared to variant 1. The resulting protein (isoform 2) is shorter but has the same N- and C-termini compared to isoform 1. Variants 2, 3, 5, and 6 all encode the same isoform (2). ##Evidence-Data-START## Transcript exon combination :: AB072336.1, SRR1660821.145734.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGCTCACTG 
AGTGTGGCCC TGGACAAGCT TCGGACCCAG AAGAGTGACC CAGAGCACCC GGGGAGGCTG 
GTAGTAGAGG CCGCACTAGG AAATGTAGCC CGGGCTCTAG ATCTTCTGCG GAGGCATCCA 
GAGCAGGTGG ACACCAAGAA CCAGGGTAGA ACTGCCCTGC AGGTGGCTGC TTACCTGGGC 
CAGGTAGAGC TGGTGCGGCT GCTGCTGCAG GCAAGAGCAA GTATGGACCT GCCGGATGAT 
GAGGGCAACA CTGTGCTGCA CTACACAGCC ATGGGGAACC AGCCTGAGGC CACAAGGGTG 
CTCCTGAGTG CAGGGTGTGC AGTGGATGCT CGGAATGGCA CACGCAGCAC AGCCCTACAT 
GTGGCTGTAC AGAGGGGCTT CCTAGAGGTG GTAAAGATCC TGTGTGAACG TGGTTGTGAT 
GTCAACTTGC CGGATGCGCA TGCAGACACA CCCCTGCATT CTGCCATCTC TGCTGGTGCT 
GGTGCCAGCA GCATTGTTGA AGTCCTCACC GAGGTCCCTG GCATCGATGT CACTGCTACC 
AATAGCCAGG GCTTCACACT GCTACACCAT GCATCCCTCA AGGGCCATGT GCTAGCAGTC 
AGGAAGATTC TGGCAAGGGC CCGGCAGCTG GTGGATGCCA AGAAGGAGGA TGGCTTTACC 
GCACTGCATC TGGCAGCCCT CAACAACCAC CGGGAGGTGG CCCAGGTCCT AATCCGAGAG 
GGTCGCTGTG ATGTGAATGT GCGGAACCGG AAGCTTCAGT CTCCACTTCA TCTGGCTGTA 
CAGCAGGCCC ACCTGGGGCT GGTACCGCTG CTAGTGGACG CAGGCTGCAG TGTCAACACG 
GAGGACGAGG AGGGTGACAC GGCCCTGCAT GTGGCACTAC AGCGTCACCA GCTGTTGCCC 
CTTGTGGCTG ACAGGGCTGG GGGAGACCCA GGGCCCTTGC AGCTGCTGTC AAGGCTACAG 
GCCTCAGGCC TCCCAGGCTG CACAGAGTTG ACTGTAGGCG CTGCAGTGGC CTGCTTCCTG 
GCGTTGGAGG GTGCTGATGT GAGCTACGCA AACCACCGCG GCCGGAGCCC ACTGGACCTG 
GCCACAGAGG GCCGAGTGCT CAAGGCCTTG CAGGGCTGTG CCCAGCGCTT CAGGGAGCGA 
CAGGCAGGTG GCGGTGGGGG TGTGCCCCCG GGCCCCCGGC ATGTGCTGAG TACTCCTAAC 
ACCGTGACGA ACCTGCACGT GTCTGGCACG GCAGGGCCAG AAGCTGCCGA GTGCCTGGTG 
TGCTCGGAGC TGGCATTGCT AATTCTGTTC TCACCGTGTC AGCACCGCAC AGTGTGTGAG 
GAGTGCGCTC GAAGGATGAA GAAGTGTATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTC 
CGCCCAGACG GCTCAGAGGT GGTAAATGCC ATCCAGGTGC CCGGCCCGCC TCGGCAACTG 
GTGGAGGAGC TACAGAGCCG CTACAGGCAG ATGGAGGAGC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT GGTGTTCCAG TGCGGCCATG GAGCGTGCGC GCCCTGTGGC 
GCCGCGCTTA ACGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT TCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_660106.2
CDS	860..3625
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_145124.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145124.3

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGCTCACTG 
AGTGTGGCCC TGGACAAGCT TCGGACCCAG AAGAGTGACC CAGAGCACCC GGGGAGGCTG 
GTAGTAGAGG CCGCACTAGG AAATGTAGCC CGGGCTCTAG ATCTTCTGCG GAGGCATCCA 
GAGCAGGTGG ACACCAAGAA CCAGGGTAGA ACTGCCCTGC AGGTGGCTGC TTACCTGGGC 
CAGGTAGAGC TGGTGCGGCT GCTGCTGCAG GCAAGAGCAA GTATGGACCT GCCGGATGAT 
GAGGGCAACA CTGTGCTGCA CTACACAGCC ATGGGGAACC AGCCTGAGGC CACAAGGGTG 
CTCCTGAGTG CAGGGTGTGC AGTGGATGCT CGGAATGGCA CACGCAGCAC AGCCCTACAT 
GTGGCTGTAC AGAGGGGCTT CCTAGAGGTG GTAAAGATCC TGTGTGAACG TGGTTGTGAT 
GTCAACTTGC CGGATGCGCA TGCAGACACA CCCCTGCATT CTGCCATCTC TGCTGGTGCT 
GGTGCCAGCA GCATTGTTGA AGTCCTCACC GAGGTCCCTG GCATCGATGT CACTGCTACC 
AATAGCCAGG GCTTCACACT GCTACACCAT GCATCCCTCA AGGGCCATGT GCTAGCAGTC 
AGGAAGATTC TGGCAAGGGC CCGGCAGCTG GTGGATGCCA AGAAGGAGGA TGGCTTTACC 
GCACTGCATC TGGCAGCCCT CAACAACCAC CGGGAGGTGG CCCAGGTCCT AATCCGAGAG 
GGTCGCTGTG ATGTGAATGT GCGGAACCGG AAGCTTCAGT CTCCACTTCA TCTGGCTGTA 
CAGCAGGCCC ACCTGGGGCT GGTACCGCTG CTAGTGGACG CAGGCTGCAG TGTCAACACG 
GAGGACGAGG AGGGTGACAC GGCCCTGCAT GTGGCACTAC AGCGTCACCA GCTGTTGCCC 
CTTGTGGCTG ACAGGGCTGG GGGAGACCCA GGGCCCTTGC AGCTGCTGTC AAGGCTACAG 
GCCTCAGGCC TCCCAGGCTG CACAGAGTTG ACTGTAGGCG CTGCAGTGGC CTGCTTCCTG 
GCGTTGGAGG GTGCTGATGT GAGCTACGCA AACCACCGCG GCCGGAGCCC ACTGGACCTG 
GCCACAGAGG GCCGAGTGCT CAAGGCCTTG CAGGGCTGTG CCCAGCGCTT CAGGGAGCGA 
CAGGCAGGTG GCGGTGGGGG TGTGCCCCCG GGCCCCCGGC ATGTGCTGAG TACTCCTAAC 
ACCGTGACGA ACCTGCACGT GTCTGGCACG GCAGGGCCAG AAGCTGCCGA GTGCCTGGTG 
TGCTCGGAGC TGGCATTGCT AATTCTGTTC TCACCGTGTC AGCACCGCAC AGTGTGTGAG 
GAGTGCGCTC GAAGGATGAA GAAGTGTATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTC 
CGCCCAGACG GCTCAGAGGT GGTAAATGCC ATCCAGGTGC CCGGCCCGCC TCGGCAACTG 
GTGGAGGAGC TACAGAGCCG CTACAGGCAG ATGGAGGAGC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT GGTGTTCCAG TGCGGCCATG GAGCGTGCGC GCCCTGTGGC 
GCCGCGCTTA ACGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT TCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( NM_001369162.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001356091.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OMu06024
Clone ID Related Accession (Same CDS sequence)	NM_001369163.1 , NM_001256108.2 , NM_145124.3 , NM_001369162.1
Accession Version	NM_001369162.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627405.17. Transcript Variant: This variant (5), as well as variants 2, 3, and 6, encodes isoform 2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.2765015.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGCTCACTG 
AGTGTGGCCC TGGACAAGCT TCGGACCCAG AAGAGTGACC CAGAGCACCC GGGGAGGCTG 
GTAGTAGAGG CCGCACTAGG AAATGTAGCC CGGGCTCTAG ATCTTCTGCG GAGGCATCCA 
GAGCAGGTGG ACACCAAGAA CCAGGGTAGA ACTGCCCTGC AGGTGGCTGC TTACCTGGGC 
CAGGTAGAGC TGGTGCGGCT GCTGCTGCAG GCAAGAGCAA GTATGGACCT GCCGGATGAT 
GAGGGCAACA CTGTGCTGCA CTACACAGCC ATGGGGAACC AGCCTGAGGC CACAAGGGTG 
CTCCTGAGTG CAGGGTGTGC AGTGGATGCT CGGAATGGCA CACGCAGCAC AGCCCTACAT 
GTGGCTGTAC AGAGGGGCTT CCTAGAGGTG GTAAAGATCC TGTGTGAACG TGGTTGTGAT 
GTCAACTTGC CGGATGCGCA TGCAGACACA CCCCTGCATT CTGCCATCTC TGCTGGTGCT 
GGTGCCAGCA GCATTGTTGA AGTCCTCACC GAGGTCCCTG GCATCGATGT CACTGCTACC 
AATAGCCAGG GCTTCACACT GCTACACCAT GCATCCCTCA AGGGCCATGT GCTAGCAGTC 
AGGAAGATTC TGGCAAGGGC CCGGCAGCTG GTGGATGCCA AGAAGGAGGA TGGCTTTACC 
GCACTGCATC TGGCAGCCCT CAACAACCAC CGGGAGGTGG CCCAGGTCCT AATCCGAGAG 
GGTCGCTGTG ATGTGAATGT GCGGAACCGG AAGCTTCAGT CTCCACTTCA TCTGGCTGTA 
CAGCAGGCCC ACCTGGGGCT GGTACCGCTG CTAGTGGACG CAGGCTGCAG TGTCAACACG 
GAGGACGAGG AGGGTGACAC GGCCCTGCAT GTGGCACTAC AGCGTCACCA GCTGTTGCCC 
CTTGTGGCTG ACAGGGCTGG GGGAGACCCA GGGCCCTTGC AGCTGCTGTC AAGGCTACAG 
GCCTCAGGCC TCCCAGGCTG CACAGAGTTG ACTGTAGGCG CTGCAGTGGC CTGCTTCCTG 
GCGTTGGAGG GTGCTGATGT GAGCTACGCA AACCACCGCG GCCGGAGCCC ACTGGACCTG 
GCCACAGAGG GCCGAGTGCT CAAGGCCTTG CAGGGCTGTG CCCAGCGCTT CAGGGAGCGA 
CAGGCAGGTG GCGGTGGGGG TGTGCCCCCG GGCCCCCGGC ATGTGCTGAG TACTCCTAAC 
ACCGTGACGA ACCTGCACGT GTCTGGCACG GCAGGGCCAG AAGCTGCCGA GTGCCTGGTG 
TGCTCGGAGC TGGCATTGCT AATTCTGTTC TCACCGTGTC AGCACCGCAC AGTGTGTGAG 
GAGTGCGCTC GAAGGATGAA GAAGTGTATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTC 
CGCCCAGACG GCTCAGAGGT GGTAAATGCC ATCCAGGTGC CCGGCCCGCC TCGGCAACTG 
GTGGAGGAGC TACAGAGCCG CTACAGGCAG ATGGAGGAGC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT GGTGTTCCAG TGCGGCCATG GAGCGTGCGC GCCCTGTGGC 
GCCGCGCTTA ACGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT TCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356091.1
CDS	669..3434
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_001369162.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369162.1

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGCTCACTG 
AGTGTGGCCC TGGACAAGCT TCGGACCCAG AAGAGTGACC CAGAGCACCC GGGGAGGCTG 
GTAGTAGAGG CCGCACTAGG AAATGTAGCC CGGGCTCTAG ATCTTCTGCG GAGGCATCCA 
GAGCAGGTGG ACACCAAGAA CCAGGGTAGA ACTGCCCTGC AGGTGGCTGC TTACCTGGGC 
CAGGTAGAGC TGGTGCGGCT GCTGCTGCAG GCAAGAGCAA GTATGGACCT GCCGGATGAT 
GAGGGCAACA CTGTGCTGCA CTACACAGCC ATGGGGAACC AGCCTGAGGC CACAAGGGTG 
CTCCTGAGTG CAGGGTGTGC AGTGGATGCT CGGAATGGCA CACGCAGCAC AGCCCTACAT 
GTGGCTGTAC AGAGGGGCTT CCTAGAGGTG GTAAAGATCC TGTGTGAACG TGGTTGTGAT 
GTCAACTTGC CGGATGCGCA TGCAGACACA CCCCTGCATT CTGCCATCTC TGCTGGTGCT 
GGTGCCAGCA GCATTGTTGA AGTCCTCACC GAGGTCCCTG GCATCGATGT CACTGCTACC 
AATAGCCAGG GCTTCACACT GCTACACCAT GCATCCCTCA AGGGCCATGT GCTAGCAGTC 
AGGAAGATTC TGGCAAGGGC CCGGCAGCTG GTGGATGCCA AGAAGGAGGA TGGCTTTACC 
GCACTGCATC TGGCAGCCCT CAACAACCAC CGGGAGGTGG CCCAGGTCCT AATCCGAGAG 
GGTCGCTGTG ATGTGAATGT GCGGAACCGG AAGCTTCAGT CTCCACTTCA TCTGGCTGTA 
CAGCAGGCCC ACCTGGGGCT GGTACCGCTG CTAGTGGACG CAGGCTGCAG TGTCAACACG 
GAGGACGAGG AGGGTGACAC GGCCCTGCAT GTGGCACTAC AGCGTCACCA GCTGTTGCCC 
CTTGTGGCTG ACAGGGCTGG GGGAGACCCA GGGCCCTTGC AGCTGCTGTC AAGGCTACAG 
GCCTCAGGCC TCCCAGGCTG CACAGAGTTG ACTGTAGGCG CTGCAGTGGC CTGCTTCCTG 
GCGTTGGAGG GTGCTGATGT GAGCTACGCA AACCACCGCG GCCGGAGCCC ACTGGACCTG 
GCCACAGAGG GCCGAGTGCT CAAGGCCTTG CAGGGCTGTG CCCAGCGCTT CAGGGAGCGA 
CAGGCAGGTG GCGGTGGGGG TGTGCCCCCG GGCCCCCGGC ATGTGCTGAG TACTCCTAAC 
ACCGTGACGA ACCTGCACGT GTCTGGCACG GCAGGGCCAG AAGCTGCCGA GTGCCTGGTG 
TGCTCGGAGC TGGCATTGCT AATTCTGTTC TCACCGTGTC AGCACCGCAC AGTGTGTGAG 
GAGTGCGCTC GAAGGATGAA GAAGTGTATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTC 
CGCCCAGACG GCTCAGAGGT GGTAAATGCC ATCCAGGTGC CCGGCCCGCC TCGGCAACTG 
GTGGAGGAGC TACAGAGCCG CTACAGGCAG ATGGAGGAGC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT GGTGTTCCAG TGCGGCCATG GAGCGTGCGC GCCCTGTGGC 
GCCGCGCTTA ACGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT TCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( NM_001369164.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001356093.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OMu34473
Clone ID Related Accession (Same CDS sequence)	XM_006539252.2 , NM_001369164.1 , NM_001369165.1
Accession Version	NM_001369164.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627405.17. On Mar 21, 2019 this sequence version replaced XM_006539252.2. Transcript Variant: This variant (7), as well as variant 8, encodes the longest isoform (3). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.4797018.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GCTCACTGAG TGTGGCCCTG 
GACAAGCTTC GGACCCAGAA GAGTGACCCA GAGCACCCGG GGAGGCTGGT AGTAGAGGCC 
GCACTAGGAA ATGTAGCCCG GGCTCTAGAT CTTCTGCGGA GGCATCCAGA GCAGGTGGAC 
ACCAAGAACC AGGGTAGAAC TGCCCTGCAG GTGGCTGCTT ACCTGGGCCA GGTAGAGCTG 
GTGCGGCTGC TGCTGCAGGC AAGAGCAAGT ATGGACCTGC CGGATGATGA GGGCAACACT 
GTGCTGCACT ACACAGCCAT GGGGAACCAG CCTGAGGCCA CAAGGGTGCT CCTGAGTGCA 
GGGTGTGCAG TGGATGCTCG GAATGGCACA CGCAGCACAG CCCTACATGT GGCTGTACAG 
AGGGGCTTCC TAGAGGTGGT AAAGATCCTG TGTGAACGTG GTTGTGATGT CAACTTGCCG 
GATGCGCATG CAGACACACC CCTGCATTCT GCCATCTCTG CTGGTGCTGG TGCCAGCAGC 
ATTGTTGAAG TCCTCACCGA GGTCCCTGGC ATCGATGTCA CTGCTACCAA TAGCCAGGGC 
TTCACACTGC TACACCATGC ATCCCTCAAG GGCCATGTGC TAGCAGTCAG GAAGATTCTG 
GCAAGGGCCC GGCAGCTGGT GGATGCCAAG AAGGAGGATG GCTTTACCGC ACTGCATCTG 
GCAGCCCTCA ACAACCACCG GGAGGTGGCC CAGGTCCTAA TCCGAGAGGG TCGCTGTGAT 
GTGAATGTGC GGAACCGGAA GCTTCAGTCT CCACTTCATC TGGCTGTACA GCAGGCCCAC 
CTGGGGCTGG TACCGCTGCT AGTGGACGCA GGCTGCAGTG TCAACACGGA GGACGAGGAG 
GGTGACACGG CCCTGCATGT GGCACTACAG CGTCACCAGC TGTTGCCCCT TGTGGCTGAC 
AGGGCTGGGG GAGACCCAGG GCCCTTGCAG CTGCTGTCAA GGCTACAGGC CTCAGGCCTC 
CCAGGCTGCA CAGAGTTGAC TGTAGGCGCT GCAGTGGCCT GCTTCCTGGC GTTGGAGGGT 
GCTGATGTGA GCTACGCAAA CCACCGCGGC CGGAGCCCAC TGGACCTGGC CACAGAGGGC 
CGAGTGCTCA AGGCCTTGCA GGGCTGTGCC CAGCGCTTCA GGGAGCGACA GGCAGGTGGC 
GGTGGGGGTG TGCCCCCGGG CCCCCGGCAT GTGCTGAGTA CTCCTAACAC CGTGACGAAC 
CTGCACGTGT CTGGCACGGC AGGGCCAGAA GCTGCCGAGT GCCTGGTGTG CTCGGAGCTG 
GCATTGCTAA TTCTGTTCTC ACCGTGTCAG CACCGCACAG TGTGTGAGGA GTGCGCTCGA 
AGGATGAAGA AGTGTATCAG GTGCCAGGTG GTCATCAGCA AGAAGCTCCG CCCAGACGGC 
TCAGAGGTGG TAAATGCCAT CCAGGTGCCC GGCCCGCCTC GGCAACTGGT GGAGGAGCTA 
CAGAGCCGCT ACAGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC 
ATCCGCCTGG TGTTCCAGTG CGGCCATGGA GCGTGCGCGC CCTGTGGCGC CGCGCTTAAC 
GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATTC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356093.1
CDS	825..3698
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_001369164.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369164.1

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GCTCACTGAG TGTGGCCCTG 
GACAAGCTTC GGACCCAGAA GAGTGACCCA GAGCACCCGG GGAGGCTGGT AGTAGAGGCC 
GCACTAGGAA ATGTAGCCCG GGCTCTAGAT CTTCTGCGGA GGCATCCAGA GCAGGTGGAC 
ACCAAGAACC AGGGTAGAAC TGCCCTGCAG GTGGCTGCTT ACCTGGGCCA GGTAGAGCTG 
GTGCGGCTGC TGCTGCAGGC AAGAGCAAGT ATGGACCTGC CGGATGATGA GGGCAACACT 
GTGCTGCACT ACACAGCCAT GGGGAACCAG CCTGAGGCCA CAAGGGTGCT CCTGAGTGCA 
GGGTGTGCAG TGGATGCTCG GAATGGCACA CGCAGCACAG CCCTACATGT GGCTGTACAG 
AGGGGCTTCC TAGAGGTGGT AAAGATCCTG TGTGAACGTG GTTGTGATGT CAACTTGCCG 
GATGCGCATG CAGACACACC CCTGCATTCT GCCATCTCTG CTGGTGCTGG TGCCAGCAGC 
ATTGTTGAAG TCCTCACCGA GGTCCCTGGC ATCGATGTCA CTGCTACCAA TAGCCAGGGC 
TTCACACTGC TACACCATGC ATCCCTCAAG GGCCATGTGC TAGCAGTCAG GAAGATTCTG 
GCAAGGGCCC GGCAGCTGGT GGATGCCAAG AAGGAGGATG GCTTTACCGC ACTGCATCTG 
GCAGCCCTCA ACAACCACCG GGAGGTGGCC CAGGTCCTAA TCCGAGAGGG TCGCTGTGAT 
GTGAATGTGC GGAACCGGAA GCTTCAGTCT CCACTTCATC TGGCTGTACA GCAGGCCCAC 
CTGGGGCTGG TACCGCTGCT AGTGGACGCA GGCTGCAGTG TCAACACGGA GGACGAGGAG 
GGTGACACGG CCCTGCATGT GGCACTACAG CGTCACCAGC TGTTGCCCCT TGTGGCTGAC 
AGGGCTGGGG GAGACCCAGG GCCCTTGCAG CTGCTGTCAA GGCTACAGGC CTCAGGCCTC 
CCAGGCTGCA CAGAGTTGAC TGTAGGCGCT GCAGTGGCCT GCTTCCTGGC GTTGGAGGGT 
GCTGATGTGA GCTACGCAAA CCACCGCGGC CGGAGCCCAC TGGACCTGGC CACAGAGGGC 
CGAGTGCTCA AGGCCTTGCA GGGCTGTGCC CAGCGCTTCA GGGAGCGACA GGCAGGTGGC 
GGTGGGGGTG TGCCCCCGGG CCCCCGGCAT GTGCTGAGTA CTCCTAACAC CGTGACGAAC 
CTGCACGTGT CTGGCACGGC AGGGCCAGAA GCTGCCGAGT GCCTGGTGTG CTCGGAGCTG 
GCATTGCTAA TTCTGTTCTC ACCGTGTCAG CACCGCACAG TGTGTGAGGA GTGCGCTCGA 
AGGATGAAGA AGTGTATCAG GTGCCAGGTG GTCATCAGCA AGAAGCTCCG CCCAGACGGC 
TCAGAGGTGG TAAATGCCAT CCAGGTGCCC GGCCCGCCTC GGCAACTGGT GGAGGAGCTA 
CAGAGCCGCT ACAGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC 
ATCCGCCTGG TGTTCCAGTG CGGCCATGGA GCGTGCGCGC CCTGTGGCGC CGCGCTTAAC 
GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATTC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( NM_001369165.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001356094.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 8, mRNA.

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CloneID	OMu34473
Clone ID Related Accession (Same CDS sequence)	XM_006539252.2 , NM_001369164.1 , NM_001369165.1
Accession Version	NM_001369165.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627405.17. Transcript Variant: This variant (8), as well as variant 7, encodes the longest isoform (3). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.1169191.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GCTCACTGAG TGTGGCCCTG 
GACAAGCTTC GGACCCAGAA GAGTGACCCA GAGCACCCGG GGAGGCTGGT AGTAGAGGCC 
GCACTAGGAA ATGTAGCCCG GGCTCTAGAT CTTCTGCGGA GGCATCCAGA GCAGGTGGAC 
ACCAAGAACC AGGGTAGAAC TGCCCTGCAG GTGGCTGCTT ACCTGGGCCA GGTAGAGCTG 
GTGCGGCTGC TGCTGCAGGC AAGAGCAAGT ATGGACCTGC CGGATGATGA GGGCAACACT 
GTGCTGCACT ACACAGCCAT GGGGAACCAG CCTGAGGCCA CAAGGGTGCT CCTGAGTGCA 
GGGTGTGCAG TGGATGCTCG GAATGGCACA CGCAGCACAG CCCTACATGT GGCTGTACAG 
AGGGGCTTCC TAGAGGTGGT AAAGATCCTG TGTGAACGTG GTTGTGATGT CAACTTGCCG 
GATGCGCATG CAGACACACC CCTGCATTCT GCCATCTCTG CTGGTGCTGG TGCCAGCAGC 
ATTGTTGAAG TCCTCACCGA GGTCCCTGGC ATCGATGTCA CTGCTACCAA TAGCCAGGGC 
TTCACACTGC TACACCATGC ATCCCTCAAG GGCCATGTGC TAGCAGTCAG GAAGATTCTG 
GCAAGGGCCC GGCAGCTGGT GGATGCCAAG AAGGAGGATG GCTTTACCGC ACTGCATCTG 
GCAGCCCTCA ACAACCACCG GGAGGTGGCC CAGGTCCTAA TCCGAGAGGG TCGCTGTGAT 
GTGAATGTGC GGAACCGGAA GCTTCAGTCT CCACTTCATC TGGCTGTACA GCAGGCCCAC 
CTGGGGCTGG TACCGCTGCT AGTGGACGCA GGCTGCAGTG TCAACACGGA GGACGAGGAG 
GGTGACACGG CCCTGCATGT GGCACTACAG CGTCACCAGC TGTTGCCCCT TGTGGCTGAC 
AGGGCTGGGG GAGACCCAGG GCCCTTGCAG CTGCTGTCAA GGCTACAGGC CTCAGGCCTC 
CCAGGCTGCA CAGAGTTGAC TGTAGGCGCT GCAGTGGCCT GCTTCCTGGC GTTGGAGGGT 
GCTGATGTGA GCTACGCAAA CCACCGCGGC CGGAGCCCAC TGGACCTGGC CACAGAGGGC 
CGAGTGCTCA AGGCCTTGCA GGGCTGTGCC CAGCGCTTCA GGGAGCGACA GGCAGGTGGC 
GGTGGGGGTG TGCCCCCGGG CCCCCGGCAT GTGCTGAGTA CTCCTAACAC CGTGACGAAC 
CTGCACGTGT CTGGCACGGC AGGGCCAGAA GCTGCCGAGT GCCTGGTGTG CTCGGAGCTG 
GCATTGCTAA TTCTGTTCTC ACCGTGTCAG CACCGCACAG TGTGTGAGGA GTGCGCTCGA 
AGGATGAAGA AGTGTATCAG GTGCCAGGTG GTCATCAGCA AGAAGCTCCG CCCAGACGGC 
TCAGAGGTGG TAAATGCCAT CCAGGTGCCC GGCCCGCCTC GGCAACTGGT GGAGGAGCTA 
CAGAGCCGCT ACAGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC 
ATCCGCCTGG TGTTCCAGTG CGGCCATGGA GCGTGCGCGC CCTGTGGCGC CGCGCTTAAC 
GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATTC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356094.1
CDS	741..3614
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_001369165.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369165.1

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GCTCACTGAG TGTGGCCCTG 
GACAAGCTTC GGACCCAGAA GAGTGACCCA GAGCACCCGG GGAGGCTGGT AGTAGAGGCC 
GCACTAGGAA ATGTAGCCCG GGCTCTAGAT CTTCTGCGGA GGCATCCAGA GCAGGTGGAC 
ACCAAGAACC AGGGTAGAAC TGCCCTGCAG GTGGCTGCTT ACCTGGGCCA GGTAGAGCTG 
GTGCGGCTGC TGCTGCAGGC AAGAGCAAGT ATGGACCTGC CGGATGATGA GGGCAACACT 
GTGCTGCACT ACACAGCCAT GGGGAACCAG CCTGAGGCCA CAAGGGTGCT CCTGAGTGCA 
GGGTGTGCAG TGGATGCTCG GAATGGCACA CGCAGCACAG CCCTACATGT GGCTGTACAG 
AGGGGCTTCC TAGAGGTGGT AAAGATCCTG TGTGAACGTG GTTGTGATGT CAACTTGCCG 
GATGCGCATG CAGACACACC CCTGCATTCT GCCATCTCTG CTGGTGCTGG TGCCAGCAGC 
ATTGTTGAAG TCCTCACCGA GGTCCCTGGC ATCGATGTCA CTGCTACCAA TAGCCAGGGC 
TTCACACTGC TACACCATGC ATCCCTCAAG GGCCATGTGC TAGCAGTCAG GAAGATTCTG 
GCAAGGGCCC GGCAGCTGGT GGATGCCAAG AAGGAGGATG GCTTTACCGC ACTGCATCTG 
GCAGCCCTCA ACAACCACCG GGAGGTGGCC CAGGTCCTAA TCCGAGAGGG TCGCTGTGAT 
GTGAATGTGC GGAACCGGAA GCTTCAGTCT CCACTTCATC TGGCTGTACA GCAGGCCCAC 
CTGGGGCTGG TACCGCTGCT AGTGGACGCA GGCTGCAGTG TCAACACGGA GGACGAGGAG 
GGTGACACGG CCCTGCATGT GGCACTACAG CGTCACCAGC TGTTGCCCCT TGTGGCTGAC 
AGGGCTGGGG GAGACCCAGG GCCCTTGCAG CTGCTGTCAA GGCTACAGGC CTCAGGCCTC 
CCAGGCTGCA CAGAGTTGAC TGTAGGCGCT GCAGTGGCCT GCTTCCTGGC GTTGGAGGGT 
GCTGATGTGA GCTACGCAAA CCACCGCGGC CGGAGCCCAC TGGACCTGGC CACAGAGGGC 
CGAGTGCTCA AGGCCTTGCA GGGCTGTGCC CAGCGCTTCA GGGAGCGACA GGCAGGTGGC 
GGTGGGGGTG TGCCCCCGGG CCCCCGGCAT GTGCTGAGTA CTCCTAACAC CGTGACGAAC 
CTGCACGTGT CTGGCACGGC AGGGCCAGAA GCTGCCGAGT GCCTGGTGTG CTCGGAGCTG 
GCATTGCTAA TTCTGTTCTC ACCGTGTCAG CACCGCACAG TGTGTGAGGA GTGCGCTCGA 
AGGATGAAGA AGTGTATCAG GTGCCAGGTG GTCATCAGCA AGAAGCTCCG CCCAGACGGC 
TCAGAGGTGG TAAATGCCAT CCAGGTGCCC GGCCCGCCTC GGCAACTGGT GGAGGAGCTA 
CAGAGCCGCT ACAGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC 
ATCCGCCTGG TGTTCCAGTG CGGCCATGGA GCGTGCGCGC CCTGTGGCGC CGCGCTTAAC 
GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATTC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_006539250.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539313.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X3, mRNA.

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CloneID	OMu34472
Clone ID Related Accession (Same CDS sequence)	XM_006539250.2 , XM_030253880.1
Accession Version	XM_006539250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGATGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAACCA CGAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG 
CACAACAGCT TCTGGGTGGG TGATGTGGTC CGGGTCATCG GTGACCTTGA CACTGTGAAG 
CGACTTCAGG CCGGACATGG TGAATGGACC GACGACATGG CTCCCGCCCT GGGCCGAGTG 
GGGAAAGTGG TGAAGGTGTT TGGAGATGGA AACTTGCGTG TGGCGGTTGG CGGTCAGCGG 
TGGACCTTCA GCCCCTCTTG CCTAGTGGCC TACCGGCCTG AGGAAGACGC CAACCTGGAT 
GTGGCTGAGC GTGCCAGAGA GAACAAAAGT GCGGCATCAG TCTCAGTGGC TGGTGGGAGG 
CAGGGCAGCC CCTGGCCAGC ACTTACCTCA ACCCTGCCCC CAGGCTCACT GAGTGTGGCC 
CTGGACAAGC TTCGGACCCA GAAGAGTGAC CCAGAGCACC CGGGGAGGCT GGTAGTAGAG 
GCCGCACTAG GAAATGTAGC CCGGGCTCTA GATCTTCTGC GGAGGCATCC AGAGCAGGTG 
GACACCAAGA ACCAGGGTAG AACTGCCCTG CAGGTGGCTG CTTACCTGGG CCAGGTAGAG 
CTGGTGCGGC TGCTGCTGCA GGCAAGAGCA AGTATGGACC TGCCGGATGA TGAGGGCAAC 
ACTGTGCTGC ACTACACAGC CATGGGGAAC CAGCCTGAGG CCACAAGGGT GCTCCTGAGT 
GCAGGGTGTG CAGTGGATGC TCGGAATGGC ACACGCAGCA CAGCCCTACA TGTGGCTGTA 
CAGAGGGGCT TCCTAGAGGT GGTAAAGATC CTGTGTGAAC GTGGTTGTGA TGTCAACTTG 
CCGGATGCGC ATGCAGACAC ACCCCTGCAT TCTGCCATCT CTGCTGGTGC TGGTGCCAGC 
AGCATTGTTG AAGTCCTCAC CGAGGTCCCT GGCATCGATG TCACTGCTAC CAATAGCCAG 
GGCTTCACAC TGCTACACCA TGCATCCCTC AAGGGCCATG TGCTAGCAGT CAGGAAGATT 
CTGGCAAGGG CCCGGCAGCT GGTGGATGCC AAGAAGGAGG ATGGCTTTAC CGCACTGCAT 
CTGGCAGCCC TCAACAACCA CCGGGAGGTG GCCCAGGTCC TAATCCGAGA GGGTCGCTGT 
GATGTGAATG TGCGGAACCG GAAGCTTCAG TCTCCACTTC ATCTGGCTGT ACAGCAGGCC 
CACCTGGGGC TGGTACCGCT GCTAGTGGAC GCAGGCTGCA GTGTCAACAC GGAGGACGAG 
GAGGGTGACA CGGCCCTGCA TGTGGCACTA CAGCGTCACC AGCTGTTGCC CCTTGTGGCT 
GACAGGGCTG GGGGAGACCC AGGGCCCTTG CAGCTGCTGT CAAGGCTACA GGCCTCAGGC 
CTCCCAGGCT GCACAGAGTT GACTGTAGGC GCTGCAGTGG CCTGCTTCCT GGCGTTGGAG 
GGTGCTGATG TGAGCTACGC AAACCACCGC GGCCGGAGCC CACTGGACCT GGCCACAGAG 
GGCCGAGTGC TCAAGGCCTT GCAGGGCTGT GCCCAGCGCT TCAGGGAGCG ACAGGCAGGT 
GGCGGTGGGG GTGTGCCCCC GGGCCCCCGG CATGTGCTGA GTACTCCTAA CACCGTGACG 
AACCTGCACG TGTCTGGCAC GGCAGGGCCA GAAGCTGCCG AGTGCCTGGT GTGCTCGGAG 
CTGGCATTGC TAATTCTGTT CTCACCGTGT CAGCACCGCA CAGTGTGTGA GGAGTGCGCT 
CGAAGGATGA AGAAGTGTAT CAGGTGCCAG GTGGTCATCA GCAAGAAGCT CCGCCCAGAC 
GGCTCAGAGG TGGTAAATGC CATCCAGGTG CCCGGCCCGC CTCGGCAACT GGTGGAGGAG 
CTACAGAGCC GCTACAGGCA GATGGAGGAG CGCATCACCT GCCCCATCTG CATCGACAGC 
CACATCCGCC TGGTGTTCCA GTGCGGCCAT GGAGCGTGCG CGCCCTGTGG CGCCGCGCTT 
AACGCCTGCC CCATCTGCCG CCAGCCCATC CGCGACCGCA TTCAGATCTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539313.1
CDS	855..3791
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_006539250.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539250.2

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGATGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAACCA CGAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG 
CACAACAGCT TCTGGGTGGG TGATGTGGTC CGGGTCATCG GTGACCTTGA CACTGTGAAG 
CGACTTCAGG CCGGACATGG TGAATGGACC GACGACATGG CTCCCGCCCT GGGCCGAGTG 
GGGAAAGTGG TGAAGGTGTT TGGAGATGGA AACTTGCGTG TGGCGGTTGG CGGTCAGCGG 
TGGACCTTCA GCCCCTCTTG CCTAGTGGCC TACCGGCCTG AGGAAGACGC CAACCTGGAT 
GTGGCTGAGC GTGCCAGAGA GAACAAAAGT GCGGCATCAG TCTCAGTGGC TGGTGGGAGG 
CAGGGCAGCC CCTGGCCAGC ACTTACCTCA ACCCTGCCCC CAGGCTCACT GAGTGTGGCC 
CTGGACAAGC TTCGGACCCA GAAGAGTGAC CCAGAGCACC CGGGGAGGCT GGTAGTAGAG 
GCCGCACTAG GAAATGTAGC CCGGGCTCTA GATCTTCTGC GGAGGCATCC AGAGCAGGTG 
GACACCAAGA ACCAGGGTAG AACTGCCCTG CAGGTGGCTG CTTACCTGGG CCAGGTAGAG 
CTGGTGCGGC TGCTGCTGCA GGCAAGAGCA AGTATGGACC TGCCGGATGA TGAGGGCAAC 
ACTGTGCTGC ACTACACAGC CATGGGGAAC CAGCCTGAGG CCACAAGGGT GCTCCTGAGT 
GCAGGGTGTG CAGTGGATGC TCGGAATGGC ACACGCAGCA CAGCCCTACA TGTGGCTGTA 
CAGAGGGGCT TCCTAGAGGT GGTAAAGATC CTGTGTGAAC GTGGTTGTGA TGTCAACTTG 
CCGGATGCGC ATGCAGACAC ACCCCTGCAT TCTGCCATCT CTGCTGGTGC TGGTGCCAGC 
AGCATTGTTG AAGTCCTCAC CGAGGTCCCT GGCATCGATG TCACTGCTAC CAATAGCCAG 
GGCTTCACAC TGCTACACCA TGCATCCCTC AAGGGCCATG TGCTAGCAGT CAGGAAGATT 
CTGGCAAGGG CCCGGCAGCT GGTGGATGCC AAGAAGGAGG ATGGCTTTAC CGCACTGCAT 
CTGGCAGCCC TCAACAACCA CCGGGAGGTG GCCCAGGTCC TAATCCGAGA GGGTCGCTGT 
GATGTGAATG TGCGGAACCG GAAGCTTCAG TCTCCACTTC ATCTGGCTGT ACAGCAGGCC 
CACCTGGGGC TGGTACCGCT GCTAGTGGAC GCAGGCTGCA GTGTCAACAC GGAGGACGAG 
GAGGGTGACA CGGCCCTGCA TGTGGCACTA CAGCGTCACC AGCTGTTGCC CCTTGTGGCT 
GACAGGGCTG GGGGAGACCC AGGGCCCTTG CAGCTGCTGT CAAGGCTACA GGCCTCAGGC 
CTCCCAGGCT GCACAGAGTT GACTGTAGGC GCTGCAGTGG CCTGCTTCCT GGCGTTGGAG 
GGTGCTGATG TGAGCTACGC AAACCACCGC GGCCGGAGCC CACTGGACCT GGCCACAGAG 
GGCCGAGTGC TCAAGGCCTT GCAGGGCTGT GCCCAGCGCT TCAGGGAGCG ACAGGCAGGT 
GGCGGTGGGG GTGTGCCCCC GGGCCCCCGG CATGTGCTGA GTACTCCTAA CACCGTGACG 
AACCTGCACG TGTCTGGCAC GGCAGGGCCA GAAGCTGCCG AGTGCCTGGT GTGCTCGGAG 
CTGGCATTGC TAATTCTGTT CTCACCGTGT CAGCACCGCA CAGTGTGTGA GGAGTGCGCT 
CGAAGGATGA AGAAGTGTAT CAGGTGCCAG GTGGTCATCA GCAAGAAGCT CCGCCCAGAC 
GGCTCAGAGG TGGTAAATGC CATCCAGGTG CCCGGCCCGC CTCGGCAACT GGTGGAGGAG 
CTACAGAGCC GCTACAGGCA GATGGAGGAG CGCATCACCT GCCCCATCTG CATCGACAGC 
CACATCCGCC TGGTGTTCCA GTGCGGCCAT GGAGCGTGCG CGCCCTGTGG CGCCGCGCTT 
AACGCCTGCC CCATCTGCCG CCAGCCCATC CGCGACCGCA TTCAGATCTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_006539252.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539315.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu34473
Clone ID Related Accession (Same CDS sequence)	XM_006539252.2 , NM_001369164.1 , NM_001369165.1
Accession Version	XM_006539252.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GCTCACTGAG TGTGGCCCTG 
GACAAGCTTC GGACCCAGAA GAGTGACCCA GAGCACCCGG GGAGGCTGGT AGTAGAGGCC 
GCACTAGGAA ATGTAGCCCG GGCTCTAGAT CTTCTGCGGA GGCATCCAGA GCAGGTGGAC 
ACCAAGAACC AGGGTAGAAC TGCCCTGCAG GTGGCTGCTT ACCTGGGCCA GGTAGAGCTG 
GTGCGGCTGC TGCTGCAGGC AAGAGCAAGT ATGGACCTGC CGGATGATGA GGGCAACACT 
GTGCTGCACT ACACAGCCAT GGGGAACCAG CCTGAGGCCA CAAGGGTGCT CCTGAGTGCA 
GGGTGTGCAG TGGATGCTCG GAATGGCACA CGCAGCACAG CCCTACATGT GGCTGTACAG 
AGGGGCTTCC TAGAGGTGGT AAAGATCCTG TGTGAACGTG GTTGTGATGT CAACTTGCCG 
GATGCGCATG CAGACACACC CCTGCATTCT GCCATCTCTG CTGGTGCTGG TGCCAGCAGC 
ATTGTTGAAG TCCTCACCGA GGTCCCTGGC ATCGATGTCA CTGCTACCAA TAGCCAGGGC 
TTCACACTGC TACACCATGC ATCCCTCAAG GGCCATGTGC TAGCAGTCAG GAAGATTCTG 
GCAAGGGCCC GGCAGCTGGT GGATGCCAAG AAGGAGGATG GCTTTACCGC ACTGCATCTG 
GCAGCCCTCA ACAACCACCG GGAGGTGGCC CAGGTCCTAA TCCGAGAGGG TCGCTGTGAT 
GTGAATGTGC GGAACCGGAA GCTTCAGTCT CCACTTCATC TGGCTGTACA GCAGGCCCAC 
CTGGGGCTGG TACCGCTGCT AGTGGACGCA GGCTGCAGTG TCAACACGGA GGACGAGGAG 
GGTGACACGG CCCTGCATGT GGCACTACAG CGTCACCAGC TGTTGCCCCT TGTGGCTGAC 
AGGGCTGGGG GAGACCCAGG GCCCTTGCAG CTGCTGTCAA GGCTACAGGC CTCAGGCCTC 
CCAGGCTGCA CAGAGTTGAC TGTAGGCGCT GCAGTGGCCT GCTTCCTGGC GTTGGAGGGT 
GCTGATGTGA GCTACGCAAA CCACCGCGGC CGGAGCCCAC TGGACCTGGC CACAGAGGGC 
CGAGTGCTCA AGGCCTTGCA GGGCTGTGCC CAGCGCTTCA GGGAGCGACA GGCAGGTGGC 
GGTGGGGGTG TGCCCCCGGG CCCCCGGCAT GTGCTGAGTA CTCCTAACAC CGTGACGAAC 
CTGCACGTGT CTGGCACGGC AGGGCCAGAA GCTGCCGAGT GCCTGGTGTG CTCGGAGCTG 
GCATTGCTAA TTCTGTTCTC ACCGTGTCAG CACCGCACAG TGTGTGAGGA GTGCGCTCGA 
AGGATGAAGA AGTGTATCAG GTGCCAGGTG GTCATCAGCA AGAAGCTCCG CCCAGACGGC 
TCAGAGGTGG TAAATGCCAT CCAGGTGCCC GGCCCGCCTC GGCAACTGGT GGAGGAGCTA 
CAGAGCCGCT ACAGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC 
ATCCGCCTGG TGTTCCAGTG CGGCCATGGA GCGTGCGCGC CCTGTGGCGC CGCGCTTAAC 
GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATTC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539315.1
CDS	855..3728
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_006539252.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539252.2

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GCTCACTGAG TGTGGCCCTG 
GACAAGCTTC GGACCCAGAA GAGTGACCCA GAGCACCCGG GGAGGCTGGT AGTAGAGGCC 
GCACTAGGAA ATGTAGCCCG GGCTCTAGAT CTTCTGCGGA GGCATCCAGA GCAGGTGGAC 
ACCAAGAACC AGGGTAGAAC TGCCCTGCAG GTGGCTGCTT ACCTGGGCCA GGTAGAGCTG 
GTGCGGCTGC TGCTGCAGGC AAGAGCAAGT ATGGACCTGC CGGATGATGA GGGCAACACT 
GTGCTGCACT ACACAGCCAT GGGGAACCAG CCTGAGGCCA CAAGGGTGCT CCTGAGTGCA 
GGGTGTGCAG TGGATGCTCG GAATGGCACA CGCAGCACAG CCCTACATGT GGCTGTACAG 
AGGGGCTTCC TAGAGGTGGT AAAGATCCTG TGTGAACGTG GTTGTGATGT CAACTTGCCG 
GATGCGCATG CAGACACACC CCTGCATTCT GCCATCTCTG CTGGTGCTGG TGCCAGCAGC 
ATTGTTGAAG TCCTCACCGA GGTCCCTGGC ATCGATGTCA CTGCTACCAA TAGCCAGGGC 
TTCACACTGC TACACCATGC ATCCCTCAAG GGCCATGTGC TAGCAGTCAG GAAGATTCTG 
GCAAGGGCCC GGCAGCTGGT GGATGCCAAG AAGGAGGATG GCTTTACCGC ACTGCATCTG 
GCAGCCCTCA ACAACCACCG GGAGGTGGCC CAGGTCCTAA TCCGAGAGGG TCGCTGTGAT 
GTGAATGTGC GGAACCGGAA GCTTCAGTCT CCACTTCATC TGGCTGTACA GCAGGCCCAC 
CTGGGGCTGG TACCGCTGCT AGTGGACGCA GGCTGCAGTG TCAACACGGA GGACGAGGAG 
GGTGACACGG CCCTGCATGT GGCACTACAG CGTCACCAGC TGTTGCCCCT TGTGGCTGAC 
AGGGCTGGGG GAGACCCAGG GCCCTTGCAG CTGCTGTCAA GGCTACAGGC CTCAGGCCTC 
CCAGGCTGCA CAGAGTTGAC TGTAGGCGCT GCAGTGGCCT GCTTCCTGGC GTTGGAGGGT 
GCTGATGTGA GCTACGCAAA CCACCGCGGC CGGAGCCCAC TGGACCTGGC CACAGAGGGC 
CGAGTGCTCA AGGCCTTGCA GGGCTGTGCC CAGCGCTTCA GGGAGCGACA GGCAGGTGGC 
GGTGGGGGTG TGCCCCCGGG CCCCCGGCAT GTGCTGAGTA CTCCTAACAC CGTGACGAAC 
CTGCACGTGT CTGGCACGGC AGGGCCAGAA GCTGCCGAGT GCCTGGTGTG CTCGGAGCTG 
GCATTGCTAA TTCTGTTCTC ACCGTGTCAG CACCGCACAG TGTGTGAGGA GTGCGCTCGA 
AGGATGAAGA AGTGTATCAG GTGCCAGGTG GTCATCAGCA AGAAGCTCCG CCCAGACGGC 
TCAGAGGTGG TAAATGCCAT CCAGGTGCCC GGCCCGCCTC GGCAACTGGT GGAGGAGCTA 
CAGAGCCGCT ACAGGCAGAT GGAGGAGCGC ATCACCTGCC CCATCTGCAT CGACAGCCAC 
ATCCGCCTGG TGTTCCAGTG CGGCCATGGA GCGTGCGCGC CCTGTGGCGC CGCGCTTAAC 
GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATTC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_006539253.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539316.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu34474
Clone ID Related Accession (Same CDS sequence)	XM_006539253.2 , XM_030253881.1 , XM_030253882.1
Accession Version	XM_006539253.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539253.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGTGCGGCA 
TCAGTCTCAG TGGCTGGTGG GAGGCAGGGC AGCCCCTGGC CAGCACTTAC CTCAACCCTG 
CCCCCAGGCT CACTGAGTGT GGCCCTGGAC AAGCTTCGGA CCCAGAAGAG TGACCCAGAG 
CACCCGGGGA GGCTGGTAGT AGAGGCCGCA CTAGGAAATG TAGCCCGGGC TCTAGATCTT 
CTGCGGAGGC ATCCAGAGCA GGTGGACACC AAGAACCAGG GTAGAACTGC CCTGCAGGTG 
GCTGCTTACC TGGGCCAGGT AGAGCTGGTG CGGCTGCTGC TGCAGGCAAG AGCAAGTATG 
GACCTGCCGG ATGATGAGGG CAACACTGTG CTGCACTACA CAGCCATGGG GAACCAGCCT 
GAGGCCACAA GGGTGCTCCT GAGTGCAGGG TGTGCAGTGG ATGCTCGGAA TGGCACACGC 
AGCACAGCCC TACATGTGGC TGTACAGAGG GGCTTCCTAG AGGTGGTAAA GATCCTGTGT 
GAACGTGGTT GTGATGTCAA CTTGCCGGAT GCGCATGCAG ACACACCCCT GCATTCTGCC 
ATCTCTGCTG GTGCTGGTGC CAGCAGCATT GTTGAAGTCC TCACCGAGGT CCCTGGCATC 
GATGTCACTG CTACCAATAG CCAGGGCTTC ACACTGCTAC ACCATGCATC CCTCAAGGGC 
CATGTGCTAG CAGTCAGGAA GATTCTGGCA AGGGCCCGGC AGCTGGTGGA TGCCAAGAAG 
GAGGATGGCT TTACCGCACT GCATCTGGCA GCCCTCAACA ACCACCGGGA GGTGGCCCAG 
GTCCTAATCC GAGAGGGTCG CTGTGATGTG AATGTGCGGA ACCGGAAGCT TCAGTCTCCA 
CTTCATCTGG CTGTACAGCA GGCCCACCTG GGGCTGGTAC CGCTGCTAGT GGACGCAGGC 
TGCAGTGTCA ACACGGAGGA CGAGGAGGGT GACACGGCCC TGCATGTGGC ACTACAGCGT 
CACCAGCTGT TGCCCCTTGT GGCTGACAGG GCTGGGGGAG ACCCAGGGCC CTTGCAGCTG 
CTGTCAAGGC TACAGGCCTC AGGCCTCCCA GGCTGCACAG AGTTGACTGT AGGCGCTGCA 
GTGGCCTGCT TCCTGGCGTT GGAGGGTGCT GATGTGAGCT ACGCAAACCA CCGCGGCCGG 
AGCCCACTGG ACCTGGCCAC AGAGGGCCGA GTGCTCAAGG CCTTGCAGGG CTGTGCCCAG 
CGCTTCAGGG AGCGACAGGC AGGTGGCGGT GGGGGTGTGC CCCCGGGCCC CCGGCATGTG 
CTGAGTACTC CTAACACCGT GACGAACCTG CACGTGTCTG GCACGGCAGG GCCAGAAGCT 
GCCGAGTGCC TGGTGTGCTC GGAGCTGGCA TTGCTAATTC TGTTCTCACC GTGTCAGCAC 
CGCACAGTGT GTGAGGAGTG CGCTCGAAGG ATGAAGAAGT GTATCAGGTG CCAGGTGGTC 
ATCAGCAAGA AGCTCCGCCC AGACGGCTCA GAGGTGGTAA ATGCCATCCA GGTGCCCGGC 
CCGCCTCGGC AACTGGTGGA GGAGCTACAG AGCCGCTACA GGCAGATGGA GGAGCGCATC 
ACCTGCCCCA TCTGCATCGA CAGCCACATC CGCCTGGTGT TCCAGTGCGG CCATGGAGCG 
TGCGCGCCCT GTGGCGCCGC GCTTAACGCC TGCCCCATCT GCCGCCAGCC CATCCGCGAC 
CGCATTCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539316.1
CDS	855..3695
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_006539253.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539253.2

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGTGCGGCA 
TCAGTCTCAG TGGCTGGTGG GAGGCAGGGC AGCCCCTGGC CAGCACTTAC CTCAACCCTG 
CCCCCAGGCT CACTGAGTGT GGCCCTGGAC AAGCTTCGGA CCCAGAAGAG TGACCCAGAG 
CACCCGGGGA GGCTGGTAGT AGAGGCCGCA CTAGGAAATG TAGCCCGGGC TCTAGATCTT 
CTGCGGAGGC ATCCAGAGCA GGTGGACACC AAGAACCAGG GTAGAACTGC CCTGCAGGTG 
GCTGCTTACC TGGGCCAGGT AGAGCTGGTG CGGCTGCTGC TGCAGGCAAG AGCAAGTATG 
GACCTGCCGG ATGATGAGGG CAACACTGTG CTGCACTACA CAGCCATGGG GAACCAGCCT 
GAGGCCACAA GGGTGCTCCT GAGTGCAGGG TGTGCAGTGG ATGCTCGGAA TGGCACACGC 
AGCACAGCCC TACATGTGGC TGTACAGAGG GGCTTCCTAG AGGTGGTAAA GATCCTGTGT 
GAACGTGGTT GTGATGTCAA CTTGCCGGAT GCGCATGCAG ACACACCCCT GCATTCTGCC 
ATCTCTGCTG GTGCTGGTGC CAGCAGCATT GTTGAAGTCC TCACCGAGGT CCCTGGCATC 
GATGTCACTG CTACCAATAG CCAGGGCTTC ACACTGCTAC ACCATGCATC CCTCAAGGGC 
CATGTGCTAG CAGTCAGGAA GATTCTGGCA AGGGCCCGGC AGCTGGTGGA TGCCAAGAAG 
GAGGATGGCT TTACCGCACT GCATCTGGCA GCCCTCAACA ACCACCGGGA GGTGGCCCAG 
GTCCTAATCC GAGAGGGTCG CTGTGATGTG AATGTGCGGA ACCGGAAGCT TCAGTCTCCA 
CTTCATCTGG CTGTACAGCA GGCCCACCTG GGGCTGGTAC CGCTGCTAGT GGACGCAGGC 
TGCAGTGTCA ACACGGAGGA CGAGGAGGGT GACACGGCCC TGCATGTGGC ACTACAGCGT 
CACCAGCTGT TGCCCCTTGT GGCTGACAGG GCTGGGGGAG ACCCAGGGCC CTTGCAGCTG 
CTGTCAAGGC TACAGGCCTC AGGCCTCCCA GGCTGCACAG AGTTGACTGT AGGCGCTGCA 
GTGGCCTGCT TCCTGGCGTT GGAGGGTGCT GATGTGAGCT ACGCAAACCA CCGCGGCCGG 
AGCCCACTGG ACCTGGCCAC AGAGGGCCGA GTGCTCAAGG CCTTGCAGGG CTGTGCCCAG 
CGCTTCAGGG AGCGACAGGC AGGTGGCGGT GGGGGTGTGC CCCCGGGCCC CCGGCATGTG 
CTGAGTACTC CTAACACCGT GACGAACCTG CACGTGTCTG GCACGGCAGG GCCAGAAGCT 
GCCGAGTGCC TGGTGTGCTC GGAGCTGGCA TTGCTAATTC TGTTCTCACC GTGTCAGCAC 
CGCACAGTGT GTGAGGAGTG CGCTCGAAGG ATGAAGAAGT GTATCAGGTG CCAGGTGGTC 
ATCAGCAAGA AGCTCCGCCC AGACGGCTCA GAGGTGGTAA ATGCCATCCA GGTGCCCGGC 
CCGCCTCGGC AACTGGTGGA GGAGCTACAG AGCCGCTACA GGCAGATGGA GGAGCGCATC 
ACCTGCCCCA TCTGCATCGA CAGCCACATC CGCCTGGTGT TCCAGTGCGG CCATGGAGCG 
TGCGCGCCCT GTGGCGCCGC GCTTAACGCC TGCCCCATCT GCCGCCAGCC CATCCGCGAC 
CGCATTCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_006539255.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539318.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X10, mRNA.

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CloneID	OMu34475
Clone ID Related Accession (Same CDS sequence)	XM_006539255.2
Accession Version	XM_006539255.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539255.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTGGA AGTGCCGCGT CTGCTTTGAC TATGACCTCT GCACACAGTG CTACATGCAC 
AACAAGCATG ACCTGACCCA TGCCTTCGAG CGCTATGAGA CATCTCACTC ACGCCCGGTT 
ACGCTGAGCC CCCGACAGGG CCTCCCTCGG ATCCCACTGA GGGGCATCTT TCAAGGAGCT 
AAGGTGGTAC GAGGCCCTGA CTGGGAATGG GGCTCACAAG ATGGAGGGGA AGGCAAGACA 
GGTCGTGTGG TGGACATCCG TGGCTGGGAT GTGGAGACAG GCCGAAGTGT GGCCAGTGTG 
ACGTGGGCAG ATGGAACCAC GAACGTGTAC CGAGTGGGCC ACAAGGGCAA GGTGGATCTC 
AGATGTGTTG GTGAGGCAGC TGGCGGCTTT TACTACAAGG AGCACCTCCC AAAGCTTGGC 
AAGCCAGCAG AGCTGCAGCG CAGGGTGAGT GCTGATGGCC AGCCCTTCCA GCGTGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACAGATGTC CTAAGGGATA TGCAAGAAGG CCATGGTGGC 
TGGAACCCTA GGATGGCAGA GTTTATCGGA CAGATGGGCA CCGTGCACCG CATCACAGAC 
CGTGGGGACG TGCGTGTGCA GTTCAACCAC GAGACCCGCT GGACCTTCCA CCCTGGGGCT 
CTCACCAAGC ACAACAGCTT CTGGGTGGGT GATGTGGTCC GGGTCATCGG TGACCTTGAC 
ACTGTGAAGC GACTTCAGGC CGGACATGGT GAATGGACCG ACGACATGGC TCCCGCCCTG 
GGCCGAGTGG GGAAAGTGGT GAAGGTGTTT GGAGATGGAA ACTTGCGTGT GGCGGTTGGC 
GGTCAGCGGT GGACCTTCAG CCCCTCTTGC CTAGTGGCCT ACCGGCCTGA GGAAGACGCC 
AACCTGGATG TGGCTGAGCG TGCCAGAGAG AACAAAAGTG CGGCATCAGT CTCAGTGGCT 
GGTGGGAGGC AGGGCAGCCC CTGGCCAGCA CTTACCTCAA CCCTGCCCCC AGGCTCACTG 
AGTGTGGCCC TGGACAAGCT TCGGACCCAG AAGAGTGACC CAGAGCACCC GGGGAGGCTG 
GTAGTAGAGG CCGCACTAGG AAATGTAGCC CGGGCTCTAG ATCTTCTGCG GAGGCATCCA 
GAGCAGGTGG ACACCAAGAA CCAGGGTAGA ACTGCCCTGC AGGTGGCTGC TTACCTGGGC 
CAGGTAGAGC TGGTGCGGCT GCTGCTGCAG GCAAGAGCAA GTATGGACCT GCCGGATGAT 
GAGGGCAACA CTGTGCTGCA CTACACAGCC ATGGGGAACC AGCCTGAGGC CACAAGGGTG 
CTCCTGAGTG CAGGGTGTGC AGTGGATGCT CGGAATGGCA CACGCAGCAC AGCCCTACAT 
GTGGCTGTAC AGAGGGGCTT CCTAGAGGTG GTAAAGATCC TGTGTGAACG TGGTTGTGAT 
GTCAACTTGC CGGATGCGCA TGCAGACACA CCCCTGCATT CTGCCATCTC TGCTGGTGCT 
GGTGCCAGCA GCATTGTTGA AGTCCTCACC GAGGTCCCTG GCATCGATGT CACTGCTACC 
AATAGCCAGG GCTTCACACT GCTACACCAT GCATCCCTCA AGGGCCATGT GCTAGCAGTC 
AGGAAGATTC TGGCAAGGGC CCGGCAGCTG GTGGATGCCA AGAAGGAGGA TGGCTTTACC 
GCACTGCATC TGGCAGCCCT CAACAACCAC CGGGAGGTGG CCCAGGTCCT AATCCGAGAG 
GGTCGCTGTG ATGTGAATGT GCGGAACCGG AAGCTTCAGT CTCCACTTCA TCTGGCTGTA 
CAGCAGGCCC ACCTGGGGCT GGTACCGCTG CTAGTGGACG CAGGCTGCAG TGTCAACACG 
GAGGACGAGG AGGGTGACAC GGCCCTGCAT GTGGCACTAC AGCGTCACCA GCTGTTGCCC 
CTTGTGGCTG ACAGGGCTGG GGGAGACCCA GGGCCCTTGC AGCTGCTGTC AAGGCTACAG 
GCCTCAGGCC TCCCAGGCTG CACAGAGTTG ACTGTAGGCG CTGCAGTGGC CTGCTTCCTG 
GCGTTGGAGG GTGCTGATGT GAGCTACGCA AACCACCGCG GCCGGAGCCC ACTGGACCTG 
GCCACAGAGG GCCGAGTGCT CAAGGCCTTG CAGGGCTGTG CCCAGCGCTT CAGGGAGCGA 
CAGGCAGGTG GCGGTGGGGG TGTGCCCCCG GGCCCCCGGC ATGTGCTGAG TACTCCTAAC 
ACCGTGACGA ACCTGCACGT GTCTGGCACG GCAGGGCCAG AAGCTGCCGA GTGCCTGGTG 
TGCTCGGAGC TGGCATTGCT AATTCTGTTC TCACCGTGTC AGCACCGCAC AGTGTGTGAG 
GAGTGCGCTC GAAGGATGAA GAAGTGTATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTC 
CGCCCAGACG GCTCAGAGGT GGTAAATGCC ATCCAGGTGC CCGGCCCGCC TCGGCAACTG 
GTGGAGGAGC TACAGAGCCG CTACAGGCAG ATGGAGGAGC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT GGTGTTCCAG TGCGGCCATG GAGCGTGCGC GCCCTGTGGC 
GCCGCGCTTA ACGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT TCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539318.1
CDS	941..3586
Translation	MRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_006539255.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539255.2

ORF Insert Sequence:

ATGCGTTGGA AGTGCCGCGT CTGCTTTGAC TATGACCTCT GCACACAGTG CTACATGCAC 
AACAAGCATG ACCTGACCCA TGCCTTCGAG CGCTATGAGA CATCTCACTC ACGCCCGGTT 
ACGCTGAGCC CCCGACAGGG CCTCCCTCGG ATCCCACTGA GGGGCATCTT TCAAGGAGCT 
AAGGTGGTAC GAGGCCCTGA CTGGGAATGG GGCTCACAAG ATGGAGGGGA AGGCAAGACA 
GGTCGTGTGG TGGACATCCG TGGCTGGGAT GTGGAGACAG GCCGAAGTGT GGCCAGTGTG 
ACGTGGGCAG ATGGAACCAC GAACGTGTAC CGAGTGGGCC ACAAGGGCAA GGTGGATCTC 
AGATGTGTTG GTGAGGCAGC TGGCGGCTTT TACTACAAGG AGCACCTCCC AAAGCTTGGC 
AAGCCAGCAG AGCTGCAGCG CAGGGTGAGT GCTGATGGCC AGCCCTTCCA GCGTGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACAGATGTC CTAAGGGATA TGCAAGAAGG CCATGGTGGC 
TGGAACCCTA GGATGGCAGA GTTTATCGGA CAGATGGGCA CCGTGCACCG CATCACAGAC 
CGTGGGGACG TGCGTGTGCA GTTCAACCAC GAGACCCGCT GGACCTTCCA CCCTGGGGCT 
CTCACCAAGC ACAACAGCTT CTGGGTGGGT GATGTGGTCC GGGTCATCGG TGACCTTGAC 
ACTGTGAAGC GACTTCAGGC CGGACATGGT GAATGGACCG ACGACATGGC TCCCGCCCTG 
GGCCGAGTGG GGAAAGTGGT GAAGGTGTTT GGAGATGGAA ACTTGCGTGT GGCGGTTGGC 
GGTCAGCGGT GGACCTTCAG CCCCTCTTGC CTAGTGGCCT ACCGGCCTGA GGAAGACGCC 
AACCTGGATG TGGCTGAGCG TGCCAGAGAG AACAAAAGTG CGGCATCAGT CTCAGTGGCT 
GGTGGGAGGC AGGGCAGCCC CTGGCCAGCA CTTACCTCAA CCCTGCCCCC AGGCTCACTG 
AGTGTGGCCC TGGACAAGCT TCGGACCCAG AAGAGTGACC CAGAGCACCC GGGGAGGCTG 
GTAGTAGAGG CCGCACTAGG AAATGTAGCC CGGGCTCTAG ATCTTCTGCG GAGGCATCCA 
GAGCAGGTGG ACACCAAGAA CCAGGGTAGA ACTGCCCTGC AGGTGGCTGC TTACCTGGGC 
CAGGTAGAGC TGGTGCGGCT GCTGCTGCAG GCAAGAGCAA GTATGGACCT GCCGGATGAT 
GAGGGCAACA CTGTGCTGCA CTACACAGCC ATGGGGAACC AGCCTGAGGC CACAAGGGTG 
CTCCTGAGTG CAGGGTGTGC AGTGGATGCT CGGAATGGCA CACGCAGCAC AGCCCTACAT 
GTGGCTGTAC AGAGGGGCTT CCTAGAGGTG GTAAAGATCC TGTGTGAACG TGGTTGTGAT 
GTCAACTTGC CGGATGCGCA TGCAGACACA CCCCTGCATT CTGCCATCTC TGCTGGTGCT 
GGTGCCAGCA GCATTGTTGA AGTCCTCACC GAGGTCCCTG GCATCGATGT CACTGCTACC 
AATAGCCAGG GCTTCACACT GCTACACCAT GCATCCCTCA AGGGCCATGT GCTAGCAGTC 
AGGAAGATTC TGGCAAGGGC CCGGCAGCTG GTGGATGCCA AGAAGGAGGA TGGCTTTACC 
GCACTGCATC TGGCAGCCCT CAACAACCAC CGGGAGGTGG CCCAGGTCCT AATCCGAGAG 
GGTCGCTGTG ATGTGAATGT GCGGAACCGG AAGCTTCAGT CTCCACTTCA TCTGGCTGTA 
CAGCAGGCCC ACCTGGGGCT GGTACCGCTG CTAGTGGACG CAGGCTGCAG TGTCAACACG 
GAGGACGAGG AGGGTGACAC GGCCCTGCAT GTGGCACTAC AGCGTCACCA GCTGTTGCCC 
CTTGTGGCTG ACAGGGCTGG GGGAGACCCA GGGCCCTTGC AGCTGCTGTC AAGGCTACAG 
GCCTCAGGCC TCCCAGGCTG CACAGAGTTG ACTGTAGGCG CTGCAGTGGC CTGCTTCCTG 
GCGTTGGAGG GTGCTGATGT GAGCTACGCA AACCACCGCG GCCGGAGCCC ACTGGACCTG 
GCCACAGAGG GCCGAGTGCT CAAGGCCTTG CAGGGCTGTG CCCAGCGCTT CAGGGAGCGA 
CAGGCAGGTG GCGGTGGGGG TGTGCCCCCG GGCCCCCGGC ATGTGCTGAG TACTCCTAAC 
ACCGTGACGA ACCTGCACGT GTCTGGCACG GCAGGGCCAG AAGCTGCCGA GTGCCTGGTG 
TGCTCGGAGC TGGCATTGCT AATTCTGTTC TCACCGTGTC AGCACCGCAC AGTGTGTGAG 
GAGTGCGCTC GAAGGATGAA GAAGTGTATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTC 
CGCCCAGACG GCTCAGAGGT GGTAAATGCC ATCCAGGTGC CCGGCCCGCC TCGGCAACTG 
GTGGAGGAGC TACAGAGCCG CTACAGGCAG ATGGAGGAGC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT GGTGTTCCAG TGCGGCCATG GAGCGTGCGC GCCCTGTGGC 
GCCGCGCTTA ACGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT TCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_011250353.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_011248655.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OMu71497
Clone ID Related Accession (Same CDS sequence)	XM_011250353.2
Accession Version	XM_011250353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_011250353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCACCG TGCACCGCAT CACAGACCGT GGGGACGTGC GTGTGCAGTT CAACCACGAG 
ACCCGCTGGA CCTTCCACCC TGGGGCTCTC ACCAAGCACA ACAGCTTCTG GGTGGGTGAT 
GTGGTCCGGG TCATCGGTGA CCTTGACACT GTGAAGCGAC TTCAGGCCGG ACATGGTGAA 
TGGACCGACG ACATGGCTCC CGCCCTGGGC CGAGTGGGGA AAGTGGTGAA GGTGTTTGGA 
GATGGAAACT TGCGTGTGGC GGTTGGCGGT CAGCGGTGGA CCTTCAGCCC CTCTTGCCTA 
GTGGCCTACC GGCCTGAGGA AGACGCCAAC CTGGATGTGG CTGAGCGTGC CAGAGAGAAC 
AAAAGTGCGG CATCAGTCTC AGTGGCTGGT GGGAGGCAGG GCAGCCCCTG GCCAGCACTT 
ACCTCAACCC TGCCCCCAGG CTCACTGAGT GTGGCCCTGG ACAAGCTTCG GACCCAGAAG 
AGTGACCCAG AGCACCCGGG GAGGCTGGTA GTAGAGGCCG CACTAGGAAA TGTAGCCCGG 
GCTCTAGATC TTCTGCGGAG GCATCCAGAG CAGGTGGACA CCAAGAACCA GGGTAGAACT 
GCCCTGCAGG TGGCTGCTTA CCTGGGCCAG GTAGAGCTGG TGCGGCTGCT GCTGCAGGCA 
AGAGCAAGTA TGGACCTGCC GGATGATGAG GGCAACACTG TGCTGCACTA CACAGCCATG 
GGGAACCAGC CTGAGGCCAC AAGGGTGCTC CTGAGTGCAG GGTGTGCAGT GGATGCTCGG 
AATGGCACAC GCAGCACAGC CCTACATGTG GCTGTACAGA GGGGCTTCCT AGAGGTGGTA 
AAGATCCTGT GTGAACGTGG TTGTGATGTC AACTTGCCGG ATGCGCATGC AGACACACCC 
CTGCATTCTG CCATCTCTGC TGGTGCTGGT GCCAGCAGCA TTGTTGAAGT CCTCACCGAG 
GTCCCTGGCA TCGATGTCAC TGCTACCAAT AGCCAGGGCT TCACACTGCT ACACCATGCA 
TCCCTCAAGG GCCATGTGCT AGCAGTCAGG AAGATTCTGG CAAGGGCCCG GCAGCTGGTG 
GATGCCAAGA AGGAGGATGG CTTTACCGCA CTGCATCTGG CAGCCCTCAA CAACCACCGG 
GAGGTGGCCC AGGTCCTAAT CCGAGAGGGT CGCTGTGATG TGAATGTGCG GAACCGGAAG 
CTTCAGTCTC CACTTCATCT GGCTGTACAG CAGGCCCACC TGGGGCTGGT ACCGCTGCTA 
GTGGACGCAG GCTGCAGTGT CAACACGGAG GACGAGGAGG GTGACACGGC CCTGCATGTG 
GCACTACAGC GTCACCAGCT GTTGCCCCTT GTGGCTGACA GGGCTGGGGG AGACCCAGGG 
CCCTTGCAGC TGCTGTCAAG GCTACAGGCC TCAGGCCTCC CAGGCTGCAC AGAGTTGACT 
GTAGGCGCTG CAGTGGCCTG CTTCCTGGCG TTGGAGGGTG CTGATGTGAG CTACGCAAAC 
CACCGCGGCC GGAGCCCACT GGACCTGGCC ACAGAGGGCC GAGTGCTCAA GGCCTTGCAG 
GGCTGTGCCC AGCGCTTCAG GGAGCGACAG GCAGGTGGCG GTGGGGGTGT GCCCCCGGGC 
CCCCGGCATG TGCTGAGTAC TCCTAACACC GTGACGAACC TGCACGTGTC TGGCACGGCA 
GGGCCAGAAG CTGCCGAGTG CCTGGTGTGC TCGGAGCTGG CATTGCTAAT TCTGTTCTCA 
CCGTGTCAGC ACCGCACAGT GTGTGAGGAG TGCGCTCGAA GGATGAAGAA GTGTATCAGG 
TGCCAGGTGG TCATCAGCAA GAAGCTCCGC CCAGACGGCT CAGAGGTGGT AAATGCCATC 
CAGGTGCCCG GCCCGCCTCG GCAACTGGTG GAGGAGCTAC AGAGCCGCTA CAGGCAGATG 
GAGGAGCGCA TCACCTGCCC CATCTGCATC GACAGCCACA TCCGCCTGGT GTTCCAGTGC 
GGCCATGGAG CGTGCGCGCC CTGTGGCGCC GCGCTTAACG CCTGCCCCAT CTGCCGCCAG 
CCCATCCGCG ACCGCATTCA GATCTTCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011248655.1
CDS	148..2220
Translation	MGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_011250353.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011250353.2

ORF Insert Sequence:

ATGGGCACCG TGCACCGCAT CACAGACCGT GGGGACGTGC GTGTGCAGTT CAACCACGAG 
ACCCGCTGGA CCTTCCACCC TGGGGCTCTC ACCAAGCACA ACAGCTTCTG GGTGGGTGAT 
GTGGTCCGGG TCATCGGTGA CCTTGACACT GTGAAGCGAC TTCAGGCCGG ACATGGTGAA 
TGGACCGACG ACATGGCTCC CGCCCTGGGC CGAGTGGGGA AAGTGGTGAA GGTGTTTGGA 
GATGGAAACT TGCGTGTGGC GGTTGGCGGT CAGCGGTGGA CCTTCAGCCC CTCTTGCCTA 
GTGGCCTACC GGCCTGAGGA AGACGCCAAC CTGGATGTGG CTGAGCGTGC CAGAGAGAAC 
AAAAGTGCGG CATCAGTCTC AGTGGCTGGT GGGAGGCAGG GCAGCCCCTG GCCAGCACTT 
ACCTCAACCC TGCCCCCAGG CTCACTGAGT GTGGCCCTGG ACAAGCTTCG GACCCAGAAG 
AGTGACCCAG AGCACCCGGG GAGGCTGGTA GTAGAGGCCG CACTAGGAAA TGTAGCCCGG 
GCTCTAGATC TTCTGCGGAG GCATCCAGAG CAGGTGGACA CCAAGAACCA GGGTAGAACT 
GCCCTGCAGG TGGCTGCTTA CCTGGGCCAG GTAGAGCTGG TGCGGCTGCT GCTGCAGGCA 
AGAGCAAGTA TGGACCTGCC GGATGATGAG GGCAACACTG TGCTGCACTA CACAGCCATG 
GGGAACCAGC CTGAGGCCAC AAGGGTGCTC CTGAGTGCAG GGTGTGCAGT GGATGCTCGG 
AATGGCACAC GCAGCACAGC CCTACATGTG GCTGTACAGA GGGGCTTCCT AGAGGTGGTA 
AAGATCCTGT GTGAACGTGG TTGTGATGTC AACTTGCCGG ATGCGCATGC AGACACACCC 
CTGCATTCTG CCATCTCTGC TGGTGCTGGT GCCAGCAGCA TTGTTGAAGT CCTCACCGAG 
GTCCCTGGCA TCGATGTCAC TGCTACCAAT AGCCAGGGCT TCACACTGCT ACACCATGCA 
TCCCTCAAGG GCCATGTGCT AGCAGTCAGG AAGATTCTGG CAAGGGCCCG GCAGCTGGTG 
GATGCCAAGA AGGAGGATGG CTTTACCGCA CTGCATCTGG CAGCCCTCAA CAACCACCGG 
GAGGTGGCCC AGGTCCTAAT CCGAGAGGGT CGCTGTGATG TGAATGTGCG GAACCGGAAG 
CTTCAGTCTC CACTTCATCT GGCTGTACAG CAGGCCCACC TGGGGCTGGT ACCGCTGCTA 
GTGGACGCAG GCTGCAGTGT CAACACGGAG GACGAGGAGG GTGACACGGC CCTGCATGTG 
GCACTACAGC GTCACCAGCT GTTGCCCCTT GTGGCTGACA GGGCTGGGGG AGACCCAGGG 
CCCTTGCAGC TGCTGTCAAG GCTACAGGCC TCAGGCCTCC CAGGCTGCAC AGAGTTGACT 
GTAGGCGCTG CAGTGGCCTG CTTCCTGGCG TTGGAGGGTG CTGATGTGAG CTACGCAAAC 
CACCGCGGCC GGAGCCCACT GGACCTGGCC ACAGAGGGCC GAGTGCTCAA GGCCTTGCAG 
GGCTGTGCCC AGCGCTTCAG GGAGCGACAG GCAGGTGGCG GTGGGGGTGT GCCCCCGGGC 
CCCCGGCATG TGCTGAGTAC TCCTAACACC GTGACGAACC TGCACGTGTC TGGCACGGCA 
GGGCCAGAAG CTGCCGAGTG CCTGGTGTGC TCGGAGCTGG CATTGCTAAT TCTGTTCTCA 
CCGTGTCAGC ACCGCACAGT GTGTGAGGAG TGCGCTCGAA GGATGAAGAA GTGTATCAGG 
TGCCAGGTGG TCATCAGCAA GAAGCTCCGC CCAGACGGCT CAGAGGTGGT AAATGCCATC 
CAGGTGCCCG GCCCGCCTCG GCAACTGGTG GAGGAGCTAC AGAGCCGCTA CAGGCAGATG 
GAGGAGCGCA TCACCTGCCC CATCTGCATC GACAGCCACA TCCGCCTGGT GTTCCAGTGC 
GGCCATGGAG CGTGCGCGCC CTGTGGCGCC GCGCTTAACG CCTGCCCCAT CTGCCGCCAG 
CCCATCCGCG ACCGCATTCA GATCTTCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( NM_001369168.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001356097.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OMu84716
Clone ID Related Accession (Same CDS sequence)	NM_001369168.1
Accession Version	NM_001369168.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627405.17. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7974084.31217.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGCTCACTG 
AGTGTGGCCC TGGACAAGCT TCGGACCCAG AAGAGTGACC CAGAGCACCC GGGGAGGCTG 
GTAGTAGAGG CCGCACTAGG AAATGTAGCC CGGGCTCTAG ATCTTCTGCG GAGGCATCCA 
GAGCAGGTGG ACACCAAGAA CCAGGGTAGA ACTGCCCTGC AGGTGGCTGC TTACCTGGGC 
CAGGTAGAGC TGGTGCGGCT GCTGCTGCAG GCAAGAGCAA GTATGGACCT GCCGGATGAT 
GAGGGCAACA CTGTGCTGCA CTACACAGCC ATGGGGAACC AGCCTGAGGC CACAAGGGTG 
CTCCTGAGTG CAGGGTGTGC AGTGGATGCT CGGAATGGCA CACGCAGCAC AGCCCTACAT 
GTGGCTGTAC AGAGGGGCTT CCTAGAGGTG GTAAAGATCC TGTGTGAACG TGGTTGTGAT 
GTCAACTTGC CGGATGCGCA TGCAGACACA CCCCTGCATT CTGCCATCTC TGCTGGTGCT 
GGTGCCAGCA GCATTGTTGA AGTCCTCACC GAGGTCCCTG GCATCGATGT CACTGCTACC 
AATAGCCAGG GCTTCACACT GCTACACCAT GCATCCCTCA AGGGCCATGT GCTAGCAGTC 
AGGAAGATTC TGGCAAGGGC CCGGCAGCTG GTGGATGCCA AGAAGGAGGA TGGCTTTACC 
GCACTGCATC TGGCAGCCCT CAACAACCAC CGGGAGGTGG CCCAGGTCCT AATCCGAGAG 
GGTCGCTGTG ATGTGAATGT GCGGAACCGG AAGCTTCAGT CTCCACTTCA TCTGGCTGTA 
CAGCAGGCCC ACCTGGGGCT GGTACCGCTG CTAGTGGACG CAGGCTGCAG TGTCAACACG 
GAGGACGAGG AGGGTGACAC GGCCCTGCAT GTGGCACTAC AGCGTCACCA GCTGTTGCCC 
CTTGTGGCTG ACAGGGCTGG GGGAGACCCA GGGCCCTTGC AGCTGCTGTC AAGGCTACAG 
GCCTCAGGCC TCCCAGGCTG CACAGAGTTG ACTGTAGGCG CTGCAGTGGC CTGCTTCCTG 
GCGTTGGAGG GTGCTGATGT GAGCTACGCA AACCACCGCG GCCGGAGCCC ACTGGACCTG 
GCCACAGAGG GCCGAGTGCT CAAGGCCTTG CAGGGCTGTG CCCAGCGCTT CAGCACCGCA 
CAGTGTGTGA GGAGTGCGCT CGAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356097.1
CDS	825..3134
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFSTAQCVRSALEG

Target ORF information:

RefSeq Version	NM_001369168.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369168.1

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGCTCACTG 
AGTGTGGCCC TGGACAAGCT TCGGACCCAG AAGAGTGACC CAGAGCACCC GGGGAGGCTG 
GTAGTAGAGG CCGCACTAGG AAATGTAGCC CGGGCTCTAG ATCTTCTGCG GAGGCATCCA 
GAGCAGGTGG ACACCAAGAA CCAGGGTAGA ACTGCCCTGC AGGTGGCTGC TTACCTGGGC 
CAGGTAGAGC TGGTGCGGCT GCTGCTGCAG GCAAGAGCAA GTATGGACCT GCCGGATGAT 
GAGGGCAACA CTGTGCTGCA CTACACAGCC ATGGGGAACC AGCCTGAGGC CACAAGGGTG 
CTCCTGAGTG CAGGGTGTGC AGTGGATGCT CGGAATGGCA CACGCAGCAC AGCCCTACAT 
GTGGCTGTAC AGAGGGGCTT CCTAGAGGTG GTAAAGATCC TGTGTGAACG TGGTTGTGAT 
GTCAACTTGC CGGATGCGCA TGCAGACACA CCCCTGCATT CTGCCATCTC TGCTGGTGCT 
GGTGCCAGCA GCATTGTTGA AGTCCTCACC GAGGTCCCTG GCATCGATGT CACTGCTACC 
AATAGCCAGG GCTTCACACT GCTACACCAT GCATCCCTCA AGGGCCATGT GCTAGCAGTC 
AGGAAGATTC TGGCAAGGGC CCGGCAGCTG GTGGATGCCA AGAAGGAGGA TGGCTTTACC 
GCACTGCATC TGGCAGCCCT CAACAACCAC CGGGAGGTGG CCCAGGTCCT AATCCGAGAG 
GGTCGCTGTG ATGTGAATGT GCGGAACCGG AAGCTTCAGT CTCCACTTCA TCTGGCTGTA 
CAGCAGGCCC ACCTGGGGCT GGTACCGCTG CTAGTGGACG CAGGCTGCAG TGTCAACACG 
GAGGACGAGG AGGGTGACAC GGCCCTGCAT GTGGCACTAC AGCGTCACCA GCTGTTGCCC 
CTTGTGGCTG ACAGGGCTGG GGGAGACCCA GGGCCCTTGC AGCTGCTGTC AAGGCTACAG 
GCCTCAGGCC TCCCAGGCTG CACAGAGTTG ACTGTAGGCG CTGCAGTGGC CTGCTTCCTG 
GCGTTGGAGG GTGCTGATGT GAGCTACGCA AACCACCGCG GCCGGAGCCC ACTGGACCTG 
GCCACAGAGG GCCGAGTGCT CAAGGCCTTG CAGGGCTGTG CCCAGCGCTT CAGCACCGCA 
CAGTGTGTGA GGAGTGCGCT CGAAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( NM_001369166.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001356095.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OMu84717
Clone ID Related Accession (Same CDS sequence)	NM_001369166.1
Accession Version	NM_001369166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform 4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627405.17. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.3693750.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGCGTTGGA AGTGCCGCGT CTGCTTTGAC TATGACCTCT GCACACAGTG CTACATGCAC 
AACAAGCATG ACCTGACCCA TGCCTTCGAG CGCTATGAGA CATCTCACTC ACGCCCGGTT 
ACGCTGAGCC CCCGACAGGG CCTCCCTCGG ATCCCACTGA GGGGCATCTT TCAAGGAGCT 
AAGGTGGTAC GAGGCCCTGA CTGGGAATGG GGCTCACAAG ATGGAGGGGA AGGCAAGACA 
GGTCGTGTGG TGGACATCCG TGGCTGGGAT GTGGAGACAG GCCGAAGTGT GGCCAGTGTG 
ACGTGGGCAG ATGGAACCAC GAACGTGTAC CGAGTGGGCC ACAAGGGCAA GGTGGATCTC 
AGATGTGTTG GTGAGGCAGC TGGCGGCTTT TACTACAAGG AGCACCTCCC AAAGCTTGGC 
AAGCCAGCAG AGCTGCAGCG CAGGGTGAGT GCTGATGGCC AGCCCTTCCA GCGTGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACAGATGTC CTAAGGGATA TGCAAGAAGG CCATGGTGGC 
TGGAACCCTA GGATGGCAGA GATGGGCACC GTGCACCGCA TCACAGACCG TGGGGACGTG 
CGTGTGCAGT TCAACCACGA GACCCGCTGG ACCTTCCACC CTGGGGCTCT CACCAAGCAC 
AACAGCTTCT GGGTGGGTGA TGTGGTCCGG GTCATCGGTG ACCTTGACAC TGTGAAGCGA 
CTTCAGGCCG GACATGGTGA ATGGACCGAC GACATGGCTC CCGCCCTGGG CCGAGTGGGG 
AAAGTGGTGA AGGTGTTTGG AGATGGAAAC TTGCGTGTGG CGGTTGGCGG TCAGCGGTGG 
ACCTTCAGCC CCTCTTGCCT AGTGGCCTAC CGGCCTGAGG AAGACGCCAA CCTGGATGTG 
GCTGAGCGTG CCAGAGAGAA CAAAAGCTCA CTGAGTGTGG CCCTGGACAA GCTTCGGACC 
CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG CTGGTAGTAG AGGCCGCACT AGGAAATGTA 
GCCCGGGCTC TAGATCTTCT GCGGAGGCAT CCAGAGCAGG TGGACACCAA GAACCAGGGT 
AGAACTGCCC TGCAGGTGGC TGCTTACCTG GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG 
CAGGCAAGAG CAAGTATGGA CCTGCCGGAT GATGAGGGCA ACACTGTGCT GCACTACACA 
GCCATGGGGA ACCAGCCTGA GGCCACAAGG GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT 
GCTCGGAATG GCACACGCAG CACAGCCCTA CATGTGGCTG TACAGAGGGG CTTCCTAGAG 
GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT GATGTCAACT TGCCGGATGC GCATGCAGAC 
ACACCCCTGC ATTCTGCCAT CTCTGCTGGT GCTGGTGCCA GCAGCATTGT TGAAGTCCTC 
ACCGAGGTCC CTGGCATCGA TGTCACTGCT ACCAATAGCC AGGGCTTCAC ACTGCTACAC 
CATGCATCCC TCAAGGGCCA TGTGCTAGCA GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG 
CTGGTGGATG CCAAGAAGGA GGATGGCTTT ACCGCACTGC ATCTGGCAGC CCTCAACAAC 
CACCGGGAGG TGGCCCAGGT CCTAATCCGA GAGGGTCGCT GTGATGTGAA TGTGCGGAAC 
CGGAAGCTTC AGTCTCCACT TCATCTGGCT GTACAGCAGG CCCACCTGGG GCTGGTACCG 
CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC ACGGAGGACG AGGAGGGTGA CACGGCCCTG 
CATGTGGCAC TACAGCGTCA CCAGCTGTTG CCCCTTGTGG CTGACAGGGC TGGGGGAGAC 
CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA CAGGCCTCAG GCCTCCCAGG CTGCACAGAG 
TTGACTGTAG GCGCTGCAGT GGCCTGCTTC CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC 
GCAAACCACC GCGGCCGGAG CCCACTGGAC CTGGCCACAG AGGGCCGAGT GCTCAAGGCC 
TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG CGACAGGCAG GTGGCGGTGG GGGTGTGCCC 
CCGGGCCCCC GGCATGTGCT GAGTACTCCT AACACCGTGA CGAACCTGCA CGTGTCTGGC 
ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG GTGTGCTCGG AGCTGGCATT GCTAATTCTG 
TTCTCACCGT GTCAGCACCG CACAGTGTGT GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT 
ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT 
GCCATCCAGG TGCCCGGCCC GCCTCGGCAA CTGGTGGAGG AGCTACAGAG CCGCTACAGG 
CAGATGGAGG AGCGCATCAC CTGCCCCATC TGCATCGACA GCCACATCCG CCTGGTGTTC 
CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT GGCGCCGCGC TTAACGCCTG CCCCATCTGC 
CGCCAGCCCA TCCGCGACCG CATTCAGATC TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356095.1
CDS	911..3469
Translation	MRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_001369166.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369166.1

ORF Insert Sequence:

ATGCGTTGGA AGTGCCGCGT CTGCTTTGAC TATGACCTCT GCACACAGTG CTACATGCAC 
AACAAGCATG ACCTGACCCA TGCCTTCGAG CGCTATGAGA CATCTCACTC ACGCCCGGTT 
ACGCTGAGCC CCCGACAGGG CCTCCCTCGG ATCCCACTGA GGGGCATCTT TCAAGGAGCT 
AAGGTGGTAC GAGGCCCTGA CTGGGAATGG GGCTCACAAG ATGGAGGGGA AGGCAAGACA 
GGTCGTGTGG TGGACATCCG TGGCTGGGAT GTGGAGACAG GCCGAAGTGT GGCCAGTGTG 
ACGTGGGCAG ATGGAACCAC GAACGTGTAC CGAGTGGGCC ACAAGGGCAA GGTGGATCTC 
AGATGTGTTG GTGAGGCAGC TGGCGGCTTT TACTACAAGG AGCACCTCCC AAAGCTTGGC 
AAGCCAGCAG AGCTGCAGCG CAGGGTGAGT GCTGATGGCC AGCCCTTCCA GCGTGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACAGATGTC CTAAGGGATA TGCAAGAAGG CCATGGTGGC 
TGGAACCCTA GGATGGCAGA GATGGGCACC GTGCACCGCA TCACAGACCG TGGGGACGTG 
CGTGTGCAGT TCAACCACGA GACCCGCTGG ACCTTCCACC CTGGGGCTCT CACCAAGCAC 
AACAGCTTCT GGGTGGGTGA TGTGGTCCGG GTCATCGGTG ACCTTGACAC TGTGAAGCGA 
CTTCAGGCCG GACATGGTGA ATGGACCGAC GACATGGCTC CCGCCCTGGG CCGAGTGGGG 
AAAGTGGTGA AGGTGTTTGG AGATGGAAAC TTGCGTGTGG CGGTTGGCGG TCAGCGGTGG 
ACCTTCAGCC CCTCTTGCCT AGTGGCCTAC CGGCCTGAGG AAGACGCCAA CCTGGATGTG 
GCTGAGCGTG CCAGAGAGAA CAAAAGCTCA CTGAGTGTGG CCCTGGACAA GCTTCGGACC 
CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG CTGGTAGTAG AGGCCGCACT AGGAAATGTA 
GCCCGGGCTC TAGATCTTCT GCGGAGGCAT CCAGAGCAGG TGGACACCAA GAACCAGGGT 
AGAACTGCCC TGCAGGTGGC TGCTTACCTG GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG 
CAGGCAAGAG CAAGTATGGA CCTGCCGGAT GATGAGGGCA ACACTGTGCT GCACTACACA 
GCCATGGGGA ACCAGCCTGA GGCCACAAGG GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT 
GCTCGGAATG GCACACGCAG CACAGCCCTA CATGTGGCTG TACAGAGGGG CTTCCTAGAG 
GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT GATGTCAACT TGCCGGATGC GCATGCAGAC 
ACACCCCTGC ATTCTGCCAT CTCTGCTGGT GCTGGTGCCA GCAGCATTGT TGAAGTCCTC 
ACCGAGGTCC CTGGCATCGA TGTCACTGCT ACCAATAGCC AGGGCTTCAC ACTGCTACAC 
CATGCATCCC TCAAGGGCCA TGTGCTAGCA GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG 
CTGGTGGATG CCAAGAAGGA GGATGGCTTT ACCGCACTGC ATCTGGCAGC CCTCAACAAC 
CACCGGGAGG TGGCCCAGGT CCTAATCCGA GAGGGTCGCT GTGATGTGAA TGTGCGGAAC 
CGGAAGCTTC AGTCTCCACT TCATCTGGCT GTACAGCAGG CCCACCTGGG GCTGGTACCG 
CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC ACGGAGGACG AGGAGGGTGA CACGGCCCTG 
CATGTGGCAC TACAGCGTCA CCAGCTGTTG CCCCTTGTGG CTGACAGGGC TGGGGGAGAC 
CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA CAGGCCTCAG GCCTCCCAGG CTGCACAGAG 
TTGACTGTAG GCGCTGCAGT GGCCTGCTTC CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC 
GCAAACCACC GCGGCCGGAG CCCACTGGAC CTGGCCACAG AGGGCCGAGT GCTCAAGGCC 
TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG CGACAGGCAG GTGGCGGTGG GGGTGTGCCC 
CCGGGCCCCC GGCATGTGCT GAGTACTCCT AACACCGTGA CGAACCTGCA CGTGTCTGGC 
ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG GTGTGCTCGG AGCTGGCATT GCTAATTCTG 
TTCTCACCGT GTCAGCACCG CACAGTGTGT GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT 
ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT 
GCCATCCAGG TGCCCGGCCC GCCTCGGCAA CTGGTGGAGG AGCTACAGAG CCGCTACAGG 
CAGATGGAGG AGCGCATCAC CTGCCCCATC TGCATCGACA GCCACATCCG CCTGGTGTTC 
CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT GGCGCCGCGC TTAACGCCTG CCCCATCTGC 
CGCCAGCCCA TCCGCGACCG CATTCAGATC TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( NM_001369167.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001356096.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 10, mRNA.

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CloneID	OMu84718
Clone ID Related Accession (Same CDS sequence)	NM_001369167.1
Accession Version	NM_001369167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2418bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-11
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL627405.17. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR7345562.2834852.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GCTCACTGAG TGTGGCCCTG 
GACAAGCTTC GGACCCAGAA GAGTGACCCA GAGCACCCGG GGAGGCTGGT AGTAGAGGCC 
GCACTAGGAA ATGTAGCCCG GGCTCTAGAT CTTCTGCGGA GGCATCCAGA GCAGGTGGAC 
ACCAAGAACC AGGGTAGAAC TGCCCTGCAG GTGGCTGCTT ACCTGGGCCA GGTAGAGCTG 
GTGCGGCTGC TGCTGCAGGC AAGAGCAAGT ATGGACCTGC CGGATGATGA GGGCAACACT 
GTGCTGCACT ACACAGCCAT GGGGAACCAG CCTGAGGCCA CAAGGGTGCT CCTGAGTGCA 
GGGTGTGCAG TGGATGCTCG GAATGGCACA CGCAGCACAG CCCTACATGT GGCTGTACAG 
AGGGGCTTCC TAGAGGTGGT AAAGATCCTG TGTGAACGTG GTTGTGATGT CAACTTGCCG 
GATGCGCATG CAGACACACC CCTGCATTCT GCCATCTCTG CTGGTGCTGG TGCCAGCAGC 
ATTGTTGAAG TCCTCACCGA GGTCCCTGGC ATCGATGTCA CTGCTACCAA TAGCCAGGGC 
TTCACACTGC TACACCATGC ATCCCTCAAG GGCCATGTGC TAGCAGTCAG GAAGATTCTG 
GCAAGGGCCC GGCAGCTGGT GGATGCCAAG AAGGAGGATG GCTTTACCGC ACTGCATCTG 
GCAGCCCTCA ACAACCACCG GGAGGTGGCC CAGGTCCTAA TCCGAGAGGG TCGCTGTGAT 
GTGAATGTGC GGAACCGGAA GCTTCAGTCT CCACTTCATC TGGCTGTACA GCAGGCCCAC 
CTGGGGCTGG TACCGCTGCT AGTGGACGCA GGCTGCAGTG TCAACACGGA GGACGAGGAG 
GGTGACACGG CCCTGCATGT GGCACTACAG CGTCACCAGC TGTTGCCCCT TGTGGCTGAC 
AGGGCTGGGG GAGACCCAGG GCCCTTGCAG CTGCTGTCAA GGCTACAGGC CTCAGGCCTC 
CCAGGCTGCA CAGAGTTGAC TGTAGGCGCT GCAGTGGCCT GCTTCCTGGC GTTGGAGGGT 
GCTGATGTGA GCTACGCAAA CCACCGCGGC CGGAGCCCAC TGGACCTGGC CACAGAGGGC 
CGAGTGCTCA AGGCCTTGCA GGGCTGTGCC CAGCGCTTCA GCACCGCACA GTGTGTGAGG 
AGTGCGCTCG AAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356096.1
CDS	825..3242
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFSTAQCVRSALEG

Target ORF information:

RefSeq Version	NM_001369167.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369167.1

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GCTCACTGAG TGTGGCCCTG 
GACAAGCTTC GGACCCAGAA GAGTGACCCA GAGCACCCGG GGAGGCTGGT AGTAGAGGCC 
GCACTAGGAA ATGTAGCCCG GGCTCTAGAT CTTCTGCGGA GGCATCCAGA GCAGGTGGAC 
ACCAAGAACC AGGGTAGAAC TGCCCTGCAG GTGGCTGCTT ACCTGGGCCA GGTAGAGCTG 
GTGCGGCTGC TGCTGCAGGC AAGAGCAAGT ATGGACCTGC CGGATGATGA GGGCAACACT 
GTGCTGCACT ACACAGCCAT GGGGAACCAG CCTGAGGCCA CAAGGGTGCT CCTGAGTGCA 
GGGTGTGCAG TGGATGCTCG GAATGGCACA CGCAGCACAG CCCTACATGT GGCTGTACAG 
AGGGGCTTCC TAGAGGTGGT AAAGATCCTG TGTGAACGTG GTTGTGATGT CAACTTGCCG 
GATGCGCATG CAGACACACC CCTGCATTCT GCCATCTCTG CTGGTGCTGG TGCCAGCAGC 
ATTGTTGAAG TCCTCACCGA GGTCCCTGGC ATCGATGTCA CTGCTACCAA TAGCCAGGGC 
TTCACACTGC TACACCATGC ATCCCTCAAG GGCCATGTGC TAGCAGTCAG GAAGATTCTG 
GCAAGGGCCC GGCAGCTGGT GGATGCCAAG AAGGAGGATG GCTTTACCGC ACTGCATCTG 
GCAGCCCTCA ACAACCACCG GGAGGTGGCC CAGGTCCTAA TCCGAGAGGG TCGCTGTGAT 
GTGAATGTGC GGAACCGGAA GCTTCAGTCT CCACTTCATC TGGCTGTACA GCAGGCCCAC 
CTGGGGCTGG TACCGCTGCT AGTGGACGCA GGCTGCAGTG TCAACACGGA GGACGAGGAG 
GGTGACACGG CCCTGCATGT GGCACTACAG CGTCACCAGC TGTTGCCCCT TGTGGCTGAC 
AGGGCTGGGG GAGACCCAGG GCCCTTGCAG CTGCTGTCAA GGCTACAGGC CTCAGGCCTC 
CCAGGCTGCA CAGAGTTGAC TGTAGGCGCT GCAGTGGCCT GCTTCCTGGC GTTGGAGGGT 
GCTGATGTGA GCTACGCAAA CCACCGCGGC CGGAGCCCAC TGGACCTGGC CACAGAGGGC 
CGAGTGCTCA AGGCCTTGCA GGGCTGTGCC CAGCGCTTCA GCACCGCACA GTGTGTGAGG 
AGTGCGCTCG AAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_006539251.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539314.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X5, mRNA.

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CloneID	OMu34471
Clone ID Related Accession (Same CDS sequence)	XM_006539251.3 , XM_006539254.3 , XM_006539249.3 , XM_030253879.1 , XM_006539249.4 , XM_006539251.2 , XM_006539247.2
Accession Version	XM_006539251.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539251.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG 
CGACAGGCAG GTGGCGGTGG GGGTGTGCCC CCGGGCCCCC GGCATGTGCT GAGTACTCCT 
AACACCGTGA CGAACCTGCA CGTGTCTGGC ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG 
GTGTGCTCGG AGCTGGCATT GCTAATTCTG TTCTCACCGT GTCAGCACCG CACAGTGTGT 
GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG 
CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT GCCATCCAGG TGCCCGGCCC GCCTCGGCAA 
CTGGTGGAGG AGCTACAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGCCCCATC 
TGCATCGACA GCCACATCCG CCTGGTGTTC CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT 
GGCGCCGCGC TTAACGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATTCAGATC 
TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539314.1
CDS	531..3479
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_006539251.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539251.3

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG 
CGACAGGCAG GTGGCGGTGG GGGTGTGCCC CCGGGCCCCC GGCATGTGCT GAGTACTCCT 
AACACCGTGA CGAACCTGCA CGTGTCTGGC ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG 
GTGTGCTCGG AGCTGGCATT GCTAATTCTG TTCTCACCGT GTCAGCACCG CACAGTGTGT 
GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG 
CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT GCCATCCAGG TGCCCGGCCC GCCTCGGCAA 
CTGGTGGAGG AGCTACAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGCCCCATC 
TGCATCGACA GCCACATCCG CCTGGTGTTC CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT 
GGCGCCGCGC TTAACGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATTCAGATC 
TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_006539254.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539317.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OMu34471
Clone ID Related Accession (Same CDS sequence)	XM_006539251.3 , XM_006539254.3 , XM_006539249.3 , XM_030253879.1 , XM_006539249.4 , XM_006539251.2 , XM_006539247.2
Accession Version	XM_006539254.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539254.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG 
CGACAGGCAG GTGGCGGTGG GGGTGTGCCC CCGGGCCCCC GGCATGTGCT GAGTACTCCT 
AACACCGTGA CGAACCTGCA CGTGTCTGGC ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG 
GTGTGCTCGG AGCTGGCATT GCTAATTCTG TTCTCACCGT GTCAGCACCG CACAGTGTGT 
GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG 
CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT GCCATCCAGG TGCCCGGCCC GCCTCGGCAA 
CTGGTGGAGG AGCTACAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGCCCCATC 
TGCATCGACA GCCACATCCG CCTGGTGTTC CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT 
GGCGCCGCGC TTAACGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATTCAGATC 
TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539317.1
CDS	306..3254
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_006539254.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539254.3

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG 
CGACAGGCAG GTGGCGGTGG GGGTGTGCCC CCGGGCCCCC GGCATGTGCT GAGTACTCCT 
AACACCGTGA CGAACCTGCA CGTGTCTGGC ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG 
GTGTGCTCGG AGCTGGCATT GCTAATTCTG TTCTCACCGT GTCAGCACCG CACAGTGTGT 
GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG 
CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT GCCATCCAGG TGCCCGGCCC GCCTCGGCAA 
CTGGTGGAGG AGCTACAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGCCCCATC 
TGCATCGACA GCCACATCCG CCTGGTGTTC CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT 
GGCGCCGCGC TTAACGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATTCAGATC 
TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_006539249.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539312.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu34471
Clone ID Related Accession (Same CDS sequence)	XM_006539251.3 , XM_006539254.3 , XM_006539249.3 , XM_030253879.1 , XM_006539249.4 , XM_006539251.2 , XM_006539247.2
Accession Version	XM_006539249.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539249.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG 
CGACAGGCAG GTGGCGGTGG GGGTGTGCCC CCGGGCCCCC GGCATGTGCT GAGTACTCCT 
AACACCGTGA CGAACCTGCA CGTGTCTGGC ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG 
GTGTGCTCGG AGCTGGCATT GCTAATTCTG TTCTCACCGT GTCAGCACCG CACAGTGTGT 
GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG 
CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT GCCATCCAGG TGCCCGGCCC GCCTCGGCAA 
CTGGTGGAGG AGCTACAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGCCCCATC 
TGCATCGACA GCCACATCCG CCTGGTGTTC CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT 
GGCGCCGCGC TTAACGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATTCAGATC 
TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539312.1
CDS	111..3059
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_006539249.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539249.3

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG 
CGACAGGCAG GTGGCGGTGG GGGTGTGCCC CCGGGCCCCC GGCATGTGCT GAGTACTCCT 
AACACCGTGA CGAACCTGCA CGTGTCTGGC ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG 
GTGTGCTCGG AGCTGGCATT GCTAATTCTG TTCTCACCGT GTCAGCACCG CACAGTGTGT 
GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG 
CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT GCCATCCAGG TGCCCGGCCC GCCTCGGCAA 
CTGGTGGAGG AGCTACAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGCCCCATC 
TGCATCGACA GCCACATCCG CCTGGTGTTC CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT 
GGCGCCGCGC TTAACGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATTCAGATC 
TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_030253879.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109739.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OMu34471
Clone ID Related Accession (Same CDS sequence)	XM_006539251.3 , XM_006539254.3 , XM_006539249.3 , XM_030253879.1 , XM_006539249.4 , XM_006539251.2 , XM_006539247.2
Accession Version	XM_030253879.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG 
CGACAGGCAG GTGGCGGTGG GGGTGTGCCC CCGGGCCCCC GGCATGTGCT GAGTACTCCT 
AACACCGTGA CGAACCTGCA CGTGTCTGGC ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG 
GTGTGCTCGG AGCTGGCATT GCTAATTCTG TTCTCACCGT GTCAGCACCG CACAGTGTGT 
GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG 
CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT GCCATCCAGG TGCCCGGCCC GCCTCGGCAA 
CTGGTGGAGG AGCTACAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGCCCCATC 
TGCATCGACA GCCACATCCG CCTGGTGTTC CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT 
GGCGCCGCGC TTAACGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATTCAGATC 
TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109739.1
CDS	577..3525
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_030253879.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253879.1

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG 
CGACAGGCAG GTGGCGGTGG GGGTGTGCCC CCGGGCCCCC GGCATGTGCT GAGTACTCCT 
AACACCGTGA CGAACCTGCA CGTGTCTGGC ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG 
GTGTGCTCGG AGCTGGCATT GCTAATTCTG TTCTCACCGT GTCAGCACCG CACAGTGTGT 
GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG 
CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT GCCATCCAGG TGCCCGGCCC GCCTCGGCAA 
CTGGTGGAGG AGCTACAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGCCCCATC 
TGCATCGACA GCCACATCCG CCTGGTGTTC CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT 
GGCGCCGCGC TTAACGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATTCAGATC 
TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_006539249.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539312.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OMu34471
Clone ID Related Accession (Same CDS sequence)	XM_006539251.3 , XM_006539254.3 , XM_006539249.3 , XM_030253879.1 , XM_006539249.4 , XM_006539251.2 , XM_006539247.2
Accession Version	XM_006539249.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006539249.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG 
CGACAGGCAG GTGGCGGTGG GGGTGTGCCC CCGGGCCCCC GGCATGTGCT GAGTACTCCT 
AACACCGTGA CGAACCTGCA CGTGTCTGGC ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG 
GTGTGCTCGG AGCTGGCATT GCTAATTCTG TTCTCACCGT GTCAGCACCG CACAGTGTGT 
GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG 
CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT GCCATCCAGG TGCCCGGCCC GCCTCGGCAA 
CTGGTGGAGG AGCTACAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGCCCCATC 
TGCATCGACA GCCACATCCG CCTGGTGTTC CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT 
GGCGCCGCGC TTAACGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATTCAGATC 
TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539312.1
CDS	104..3052
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_006539249.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539249.4

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG 
CGACAGGCAG GTGGCGGTGG GGGTGTGCCC CCGGGCCCCC GGCATGTGCT GAGTACTCCT 
AACACCGTGA CGAACCTGCA CGTGTCTGGC ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG 
GTGTGCTCGG AGCTGGCATT GCTAATTCTG TTCTCACCGT GTCAGCACCG CACAGTGTGT 
GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG 
CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT GCCATCCAGG TGCCCGGCCC GCCTCGGCAA 
CTGGTGGAGG AGCTACAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGCCCCATC 
TGCATCGACA GCCACATCCG CCTGGTGTTC CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT 
GGCGCCGCGC TTAACGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATTCAGATC 
TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_006539251.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539314.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu34471
Clone ID Related Accession (Same CDS sequence)	XM_006539251.3 , XM_006539254.3 , XM_006539249.3 , XM_030253879.1 , XM_006539249.4 , XM_006539251.2 , XM_006539247.2
Accession Version	XM_006539251.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539251.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG 
CGACAGGCAG GTGGCGGTGG GGGTGTGCCC CCGGGCCCCC GGCATGTGCT GAGTACTCCT 
AACACCGTGA CGAACCTGCA CGTGTCTGGC ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG 
GTGTGCTCGG AGCTGGCATT GCTAATTCTG TTCTCACCGT GTCAGCACCG CACAGTGTGT 
GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG 
CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT GCCATCCAGG TGCCCGGCCC GCCTCGGCAA 
CTGGTGGAGG AGCTACAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGCCCCATC 
TGCATCGACA GCCACATCCG CCTGGTGTTC CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT 
GGCGCCGCGC TTAACGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATTCAGATC 
TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539314.1
CDS	540..3488
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_006539251.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539251.2

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG 
CGACAGGCAG GTGGCGGTGG GGGTGTGCCC CCGGGCCCCC GGCATGTGCT GAGTACTCCT 
AACACCGTGA CGAACCTGCA CGTGTCTGGC ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG 
GTGTGCTCGG AGCTGGCATT GCTAATTCTG TTCTCACCGT GTCAGCACCG CACAGTGTGT 
GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG 
CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT GCCATCCAGG TGCCCGGCCC GCCTCGGCAA 
CTGGTGGAGG AGCTACAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGCCCCATC 
TGCATCGACA GCCACATCCG CCTGGTGTTC CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT 
GGCGCCGCGC TTAACGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATTCAGATC 
TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_006539247.2 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006539310.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu34471
Clone ID Related Accession (Same CDS sequence)	XM_006539251.3 , XM_006539254.3 , XM_006539249.3 , XM_030253879.1 , XM_006539249.4 , XM_006539251.2 , XM_006539247.2
Accession Version	XM_006539247.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006539247.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG 
CGACAGGCAG GTGGCGGTGG GGGTGTGCCC CCGGGCCCCC GGCATGTGCT GAGTACTCCT 
AACACCGTGA CGAACCTGCA CGTGTCTGGC ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG 
GTGTGCTCGG AGCTGGCATT GCTAATTCTG TTCTCACCGT GTCAGCACCG CACAGTGTGT 
GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG 
CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT GCCATCCAGG TGCCCGGCCC GCCTCGGCAA 
CTGGTGGAGG AGCTACAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGCCCCATC 
TGCATCGACA GCCACATCCG CCTGGTGTTC CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT 
GGCGCCGCGC TTAACGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATTCAGATC 
TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006539310.1
CDS	771..3719
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_006539247.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006539247.2

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGGGAG 
CGACAGGCAG GTGGCGGTGG GGGTGTGCCC CCGGGCCCCC GGCATGTGCT GAGTACTCCT 
AACACCGTGA CGAACCTGCA CGTGTCTGGC ACGGCAGGGC CAGAAGCTGC CGAGTGCCTG 
GTGTGCTCGG AGCTGGCATT GCTAATTCTG TTCTCACCGT GTCAGCACCG CACAGTGTGT 
GAGGAGTGCG CTCGAAGGAT GAAGAAGTGT ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG 
CTCCGCCCAG ACGGCTCAGA GGTGGTAAAT GCCATCCAGG TGCCCGGCCC GCCTCGGCAA 
CTGGTGGAGG AGCTACAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGCCCCATC 
TGCATCGACA GCCACATCCG CCTGGTGTTC CAGTGCGGCC ATGGAGCGTG CGCGCCCTGT 
GGCGCCGCGC TTAACGCCTG CCCCATCTGC CGCCAGCCCA TCCGCGACCG CATTCAGATC 
TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_030253880.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109740.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OMu34472
Clone ID Related Accession (Same CDS sequence)	XM_006539250.2 , XM_030253880.1
Accession Version	XM_030253880.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2937bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGATGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAACCA CGAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG 
CACAACAGCT TCTGGGTGGG TGATGTGGTC CGGGTCATCG GTGACCTTGA CACTGTGAAG 
CGACTTCAGG CCGGACATGG TGAATGGACC GACGACATGG CTCCCGCCCT GGGCCGAGTG 
GGGAAAGTGG TGAAGGTGTT TGGAGATGGA AACTTGCGTG TGGCGGTTGG CGGTCAGCGG 
TGGACCTTCA GCCCCTCTTG CCTAGTGGCC TACCGGCCTG AGGAAGACGC CAACCTGGAT 
GTGGCTGAGC GTGCCAGAGA GAACAAAAGT GCGGCATCAG TCTCAGTGGC TGGTGGGAGG 
CAGGGCAGCC CCTGGCCAGC ACTTACCTCA ACCCTGCCCC CAGGCTCACT GAGTGTGGCC 
CTGGACAAGC TTCGGACCCA GAAGAGTGAC CCAGAGCACC CGGGGAGGCT GGTAGTAGAG 
GCCGCACTAG GAAATGTAGC CCGGGCTCTA GATCTTCTGC GGAGGCATCC AGAGCAGGTG 
GACACCAAGA ACCAGGGTAG AACTGCCCTG CAGGTGGCTG CTTACCTGGG CCAGGTAGAG 
CTGGTGCGGC TGCTGCTGCA GGCAAGAGCA AGTATGGACC TGCCGGATGA TGAGGGCAAC 
ACTGTGCTGC ACTACACAGC CATGGGGAAC CAGCCTGAGG CCACAAGGGT GCTCCTGAGT 
GCAGGGTGTG CAGTGGATGC TCGGAATGGC ACACGCAGCA CAGCCCTACA TGTGGCTGTA 
CAGAGGGGCT TCCTAGAGGT GGTAAAGATC CTGTGTGAAC GTGGTTGTGA TGTCAACTTG 
CCGGATGCGC ATGCAGACAC ACCCCTGCAT TCTGCCATCT CTGCTGGTGC TGGTGCCAGC 
AGCATTGTTG AAGTCCTCAC CGAGGTCCCT GGCATCGATG TCACTGCTAC CAATAGCCAG 
GGCTTCACAC TGCTACACCA TGCATCCCTC AAGGGCCATG TGCTAGCAGT CAGGAAGATT 
CTGGCAAGGG CCCGGCAGCT GGTGGATGCC AAGAAGGAGG ATGGCTTTAC CGCACTGCAT 
CTGGCAGCCC TCAACAACCA CCGGGAGGTG GCCCAGGTCC TAATCCGAGA GGGTCGCTGT 
GATGTGAATG TGCGGAACCG GAAGCTTCAG TCTCCACTTC ATCTGGCTGT ACAGCAGGCC 
CACCTGGGGC TGGTACCGCT GCTAGTGGAC GCAGGCTGCA GTGTCAACAC GGAGGACGAG 
GAGGGTGACA CGGCCCTGCA TGTGGCACTA CAGCGTCACC AGCTGTTGCC CCTTGTGGCT 
GACAGGGCTG GGGGAGACCC AGGGCCCTTG CAGCTGCTGT CAAGGCTACA GGCCTCAGGC 
CTCCCAGGCT GCACAGAGTT GACTGTAGGC GCTGCAGTGG CCTGCTTCCT GGCGTTGGAG 
GGTGCTGATG TGAGCTACGC AAACCACCGC GGCCGGAGCC CACTGGACCT GGCCACAGAG 
GGCCGAGTGC TCAAGGCCTT GCAGGGCTGT GCCCAGCGCT TCAGGGAGCG ACAGGCAGGT 
GGCGGTGGGG GTGTGCCCCC GGGCCCCCGG CATGTGCTGA GTACTCCTAA CACCGTGACG 
AACCTGCACG TGTCTGGCAC GGCAGGGCCA GAAGCTGCCG AGTGCCTGGT GTGCTCGGAG 
CTGGCATTGC TAATTCTGTT CTCACCGTGT CAGCACCGCA CAGTGTGTGA GGAGTGCGCT 
CGAAGGATGA AGAAGTGTAT CAGGTGCCAG GTGGTCATCA GCAAGAAGCT CCGCCCAGAC 
GGCTCAGAGG TGGTAAATGC CATCCAGGTG CCCGGCCCGC CTCGGCAACT GGTGGAGGAG 
CTACAGAGCC GCTACAGGCA GATGGAGGAG CGCATCACCT GCCCCATCTG CATCGACAGC 
CACATCCGCC TGGTGTTCCA GTGCGGCCAT GGAGCGTGCG CGCCCTGTGG CGCCGCGCTT 
AACGCCTGCC CCATCTGCCG CCAGCCCATC CGCGACCGCA TTCAGATCTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109740.1
CDS	771..3707
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_030253880.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253880.1

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGATGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAACCA CGAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG 
CACAACAGCT TCTGGGTGGG TGATGTGGTC CGGGTCATCG GTGACCTTGA CACTGTGAAG 
CGACTTCAGG CCGGACATGG TGAATGGACC GACGACATGG CTCCCGCCCT GGGCCGAGTG 
GGGAAAGTGG TGAAGGTGTT TGGAGATGGA AACTTGCGTG TGGCGGTTGG CGGTCAGCGG 
TGGACCTTCA GCCCCTCTTG CCTAGTGGCC TACCGGCCTG AGGAAGACGC CAACCTGGAT 
GTGGCTGAGC GTGCCAGAGA GAACAAAAGT GCGGCATCAG TCTCAGTGGC TGGTGGGAGG 
CAGGGCAGCC CCTGGCCAGC ACTTACCTCA ACCCTGCCCC CAGGCTCACT GAGTGTGGCC 
CTGGACAAGC TTCGGACCCA GAAGAGTGAC CCAGAGCACC CGGGGAGGCT GGTAGTAGAG 
GCCGCACTAG GAAATGTAGC CCGGGCTCTA GATCTTCTGC GGAGGCATCC AGAGCAGGTG 
GACACCAAGA ACCAGGGTAG AACTGCCCTG CAGGTGGCTG CTTACCTGGG CCAGGTAGAG 
CTGGTGCGGC TGCTGCTGCA GGCAAGAGCA AGTATGGACC TGCCGGATGA TGAGGGCAAC 
ACTGTGCTGC ACTACACAGC CATGGGGAAC CAGCCTGAGG CCACAAGGGT GCTCCTGAGT 
GCAGGGTGTG CAGTGGATGC TCGGAATGGC ACACGCAGCA CAGCCCTACA TGTGGCTGTA 
CAGAGGGGCT TCCTAGAGGT GGTAAAGATC CTGTGTGAAC GTGGTTGTGA TGTCAACTTG 
CCGGATGCGC ATGCAGACAC ACCCCTGCAT TCTGCCATCT CTGCTGGTGC TGGTGCCAGC 
AGCATTGTTG AAGTCCTCAC CGAGGTCCCT GGCATCGATG TCACTGCTAC CAATAGCCAG 
GGCTTCACAC TGCTACACCA TGCATCCCTC AAGGGCCATG TGCTAGCAGT CAGGAAGATT 
CTGGCAAGGG CCCGGCAGCT GGTGGATGCC AAGAAGGAGG ATGGCTTTAC CGCACTGCAT 
CTGGCAGCCC TCAACAACCA CCGGGAGGTG GCCCAGGTCC TAATCCGAGA GGGTCGCTGT 
GATGTGAATG TGCGGAACCG GAAGCTTCAG TCTCCACTTC ATCTGGCTGT ACAGCAGGCC 
CACCTGGGGC TGGTACCGCT GCTAGTGGAC GCAGGCTGCA GTGTCAACAC GGAGGACGAG 
GAGGGTGACA CGGCCCTGCA TGTGGCACTA CAGCGTCACC AGCTGTTGCC CCTTGTGGCT 
GACAGGGCTG GGGGAGACCC AGGGCCCTTG CAGCTGCTGT CAAGGCTACA GGCCTCAGGC 
CTCCCAGGCT GCACAGAGTT GACTGTAGGC GCTGCAGTGG CCTGCTTCCT GGCGTTGGAG 
GGTGCTGATG TGAGCTACGC AAACCACCGC GGCCGGAGCC CACTGGACCT GGCCACAGAG 
GGCCGAGTGC TCAAGGCCTT GCAGGGCTGT GCCCAGCGCT TCAGGGAGCG ACAGGCAGGT 
GGCGGTGGGG GTGTGCCCCC GGGCCCCCGG CATGTGCTGA GTACTCCTAA CACCGTGACG 
AACCTGCACG TGTCTGGCAC GGCAGGGCCA GAAGCTGCCG AGTGCCTGGT GTGCTCGGAG 
CTGGCATTGC TAATTCTGTT CTCACCGTGT CAGCACCGCA CAGTGTGTGA GGAGTGCGCT 
CGAAGGATGA AGAAGTGTAT CAGGTGCCAG GTGGTCATCA GCAAGAAGCT CCGCCCAGAC 
GGCTCAGAGG TGGTAAATGC CATCCAGGTG CCCGGCCCGC CTCGGCAACT GGTGGAGGAG 
CTACAGAGCC GCTACAGGCA GATGGAGGAG CGCATCACCT GCCCCATCTG CATCGACAGC 
CACATCCGCC TGGTGTTCCA GTGCGGCCAT GGAGCGTGCG CGCCCTGTGG CGCCGCGCTT 
AACGCCTGCC CCATCTGCCG CCAGCCCATC CGCGACCGCA TTCAGATCTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_030253881.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109741.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu34474
Clone ID Related Accession (Same CDS sequence)	XM_006539253.2 , XM_030253881.1 , XM_030253882.1
Accession Version	XM_030253881.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGTGCGGCA 
TCAGTCTCAG TGGCTGGTGG GAGGCAGGGC AGCCCCTGGC CAGCACTTAC CTCAACCCTG 
CCCCCAGGCT CACTGAGTGT GGCCCTGGAC AAGCTTCGGA CCCAGAAGAG TGACCCAGAG 
CACCCGGGGA GGCTGGTAGT AGAGGCCGCA CTAGGAAATG TAGCCCGGGC TCTAGATCTT 
CTGCGGAGGC ATCCAGAGCA GGTGGACACC AAGAACCAGG GTAGAACTGC CCTGCAGGTG 
GCTGCTTACC TGGGCCAGGT AGAGCTGGTG CGGCTGCTGC TGCAGGCAAG AGCAAGTATG 
GACCTGCCGG ATGATGAGGG CAACACTGTG CTGCACTACA CAGCCATGGG GAACCAGCCT 
GAGGCCACAA GGGTGCTCCT GAGTGCAGGG TGTGCAGTGG ATGCTCGGAA TGGCACACGC 
AGCACAGCCC TACATGTGGC TGTACAGAGG GGCTTCCTAG AGGTGGTAAA GATCCTGTGT 
GAACGTGGTT GTGATGTCAA CTTGCCGGAT GCGCATGCAG ACACACCCCT GCATTCTGCC 
ATCTCTGCTG GTGCTGGTGC CAGCAGCATT GTTGAAGTCC TCACCGAGGT CCCTGGCATC 
GATGTCACTG CTACCAATAG CCAGGGCTTC ACACTGCTAC ACCATGCATC CCTCAAGGGC 
CATGTGCTAG CAGTCAGGAA GATTCTGGCA AGGGCCCGGC AGCTGGTGGA TGCCAAGAAG 
GAGGATGGCT TTACCGCACT GCATCTGGCA GCCCTCAACA ACCACCGGGA GGTGGCCCAG 
GTCCTAATCC GAGAGGGTCG CTGTGATGTG AATGTGCGGA ACCGGAAGCT TCAGTCTCCA 
CTTCATCTGG CTGTACAGCA GGCCCACCTG GGGCTGGTAC CGCTGCTAGT GGACGCAGGC 
TGCAGTGTCA ACACGGAGGA CGAGGAGGGT GACACGGCCC TGCATGTGGC ACTACAGCGT 
CACCAGCTGT TGCCCCTTGT GGCTGACAGG GCTGGGGGAG ACCCAGGGCC CTTGCAGCTG 
CTGTCAAGGC TACAGGCCTC AGGCCTCCCA GGCTGCACAG AGTTGACTGT AGGCGCTGCA 
GTGGCCTGCT TCCTGGCGTT GGAGGGTGCT GATGTGAGCT ACGCAAACCA CCGCGGCCGG 
AGCCCACTGG ACCTGGCCAC AGAGGGCCGA GTGCTCAAGG CCTTGCAGGG CTGTGCCCAG 
CGCTTCAGGG AGCGACAGGC AGGTGGCGGT GGGGGTGTGC CCCCGGGCCC CCGGCATGTG 
CTGAGTACTC CTAACACCGT GACGAACCTG CACGTGTCTG GCACGGCAGG GCCAGAAGCT 
GCCGAGTGCC TGGTGTGCTC GGAGCTGGCA TTGCTAATTC TGTTCTCACC GTGTCAGCAC 
CGCACAGTGT GTGAGGAGTG CGCTCGAAGG ATGAAGAAGT GTATCAGGTG CCAGGTGGTC 
ATCAGCAAGA AGCTCCGCCC AGACGGCTCA GAGGTGGTAA ATGCCATCCA GGTGCCCGGC 
CCGCCTCGGC AACTGGTGGA GGAGCTACAG AGCCGCTACA GGCAGATGGA GGAGCGCATC 
ACCTGCCCCA TCTGCATCGA CAGCCACATC CGCCTGGTGT TCCAGTGCGG CCATGGAGCG 
TGCGCGCCCT GTGGCGCCGC GCTTAACGCC TGCCCCATCT GCCGCCAGCC CATCCGCGAC 
CGCATTCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109741.1
CDS	771..3611
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_030253881.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253881.1

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGTGCGGCA 
TCAGTCTCAG TGGCTGGTGG GAGGCAGGGC AGCCCCTGGC CAGCACTTAC CTCAACCCTG 
CCCCCAGGCT CACTGAGTGT GGCCCTGGAC AAGCTTCGGA CCCAGAAGAG TGACCCAGAG 
CACCCGGGGA GGCTGGTAGT AGAGGCCGCA CTAGGAAATG TAGCCCGGGC TCTAGATCTT 
CTGCGGAGGC ATCCAGAGCA GGTGGACACC AAGAACCAGG GTAGAACTGC CCTGCAGGTG 
GCTGCTTACC TGGGCCAGGT AGAGCTGGTG CGGCTGCTGC TGCAGGCAAG AGCAAGTATG 
GACCTGCCGG ATGATGAGGG CAACACTGTG CTGCACTACA CAGCCATGGG GAACCAGCCT 
GAGGCCACAA GGGTGCTCCT GAGTGCAGGG TGTGCAGTGG ATGCTCGGAA TGGCACACGC 
AGCACAGCCC TACATGTGGC TGTACAGAGG GGCTTCCTAG AGGTGGTAAA GATCCTGTGT 
GAACGTGGTT GTGATGTCAA CTTGCCGGAT GCGCATGCAG ACACACCCCT GCATTCTGCC 
ATCTCTGCTG GTGCTGGTGC CAGCAGCATT GTTGAAGTCC TCACCGAGGT CCCTGGCATC 
GATGTCACTG CTACCAATAG CCAGGGCTTC ACACTGCTAC ACCATGCATC CCTCAAGGGC 
CATGTGCTAG CAGTCAGGAA GATTCTGGCA AGGGCCCGGC AGCTGGTGGA TGCCAAGAAG 
GAGGATGGCT TTACCGCACT GCATCTGGCA GCCCTCAACA ACCACCGGGA GGTGGCCCAG 
GTCCTAATCC GAGAGGGTCG CTGTGATGTG AATGTGCGGA ACCGGAAGCT TCAGTCTCCA 
CTTCATCTGG CTGTACAGCA GGCCCACCTG GGGCTGGTAC CGCTGCTAGT GGACGCAGGC 
TGCAGTGTCA ACACGGAGGA CGAGGAGGGT GACACGGCCC TGCATGTGGC ACTACAGCGT 
CACCAGCTGT TGCCCCTTGT GGCTGACAGG GCTGGGGGAG ACCCAGGGCC CTTGCAGCTG 
CTGTCAAGGC TACAGGCCTC AGGCCTCCCA GGCTGCACAG AGTTGACTGT AGGCGCTGCA 
GTGGCCTGCT TCCTGGCGTT GGAGGGTGCT GATGTGAGCT ACGCAAACCA CCGCGGCCGG 
AGCCCACTGG ACCTGGCCAC AGAGGGCCGA GTGCTCAAGG CCTTGCAGGG CTGTGCCCAG 
CGCTTCAGGG AGCGACAGGC AGGTGGCGGT GGGGGTGTGC CCCCGGGCCC CCGGCATGTG 
CTGAGTACTC CTAACACCGT GACGAACCTG CACGTGTCTG GCACGGCAGG GCCAGAAGCT 
GCCGAGTGCC TGGTGTGCTC GGAGCTGGCA TTGCTAATTC TGTTCTCACC GTGTCAGCAC 
CGCACAGTGT GTGAGGAGTG CGCTCGAAGG ATGAAGAAGT GTATCAGGTG CCAGGTGGTC 
ATCAGCAAGA AGCTCCGCCC AGACGGCTCA GAGGTGGTAA ATGCCATCCA GGTGCCCGGC 
CCGCCTCGGC AACTGGTGGA GGAGCTACAG AGCCGCTACA GGCAGATGGA GGAGCGCATC 
ACCTGCCCCA TCTGCATCGA CAGCCACATC CGCCTGGTGT TCCAGTGCGG CCATGGAGCG 
TGCGCGCCCT GTGGCGCCGC GCTTAACGCC TGCCCCATCT GCCGCCAGCC CATCCGCGAC 
CGCATTCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_030253882.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109742.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OMu34474
Clone ID Related Accession (Same CDS sequence)	XM_006539253.2 , XM_030253881.1 , XM_030253882.1
Accession Version	XM_030253882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2841bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGTGCGGCA 
TCAGTCTCAG TGGCTGGTGG GAGGCAGGGC AGCCCCTGGC CAGCACTTAC CTCAACCCTG 
CCCCCAGGCT CACTGAGTGT GGCCCTGGAC AAGCTTCGGA CCCAGAAGAG TGACCCAGAG 
CACCCGGGGA GGCTGGTAGT AGAGGCCGCA CTAGGAAATG TAGCCCGGGC TCTAGATCTT 
CTGCGGAGGC ATCCAGAGCA GGTGGACACC AAGAACCAGG GTAGAACTGC CCTGCAGGTG 
GCTGCTTACC TGGGCCAGGT AGAGCTGGTG CGGCTGCTGC TGCAGGCAAG AGCAAGTATG 
GACCTGCCGG ATGATGAGGG CAACACTGTG CTGCACTACA CAGCCATGGG GAACCAGCCT 
GAGGCCACAA GGGTGCTCCT GAGTGCAGGG TGTGCAGTGG ATGCTCGGAA TGGCACACGC 
AGCACAGCCC TACATGTGGC TGTACAGAGG GGCTTCCTAG AGGTGGTAAA GATCCTGTGT 
GAACGTGGTT GTGATGTCAA CTTGCCGGAT GCGCATGCAG ACACACCCCT GCATTCTGCC 
ATCTCTGCTG GTGCTGGTGC CAGCAGCATT GTTGAAGTCC TCACCGAGGT CCCTGGCATC 
GATGTCACTG CTACCAATAG CCAGGGCTTC ACACTGCTAC ACCATGCATC CCTCAAGGGC 
CATGTGCTAG CAGTCAGGAA GATTCTGGCA AGGGCCCGGC AGCTGGTGGA TGCCAAGAAG 
GAGGATGGCT TTACCGCACT GCATCTGGCA GCCCTCAACA ACCACCGGGA GGTGGCCCAG 
GTCCTAATCC GAGAGGGTCG CTGTGATGTG AATGTGCGGA ACCGGAAGCT TCAGTCTCCA 
CTTCATCTGG CTGTACAGCA GGCCCACCTG GGGCTGGTAC CGCTGCTAGT GGACGCAGGC 
TGCAGTGTCA ACACGGAGGA CGAGGAGGGT GACACGGCCC TGCATGTGGC ACTACAGCGT 
CACCAGCTGT TGCCCCTTGT GGCTGACAGG GCTGGGGGAG ACCCAGGGCC CTTGCAGCTG 
CTGTCAAGGC TACAGGCCTC AGGCCTCCCA GGCTGCACAG AGTTGACTGT AGGCGCTGCA 
GTGGCCTGCT TCCTGGCGTT GGAGGGTGCT GATGTGAGCT ACGCAAACCA CCGCGGCCGG 
AGCCCACTGG ACCTGGCCAC AGAGGGCCGA GTGCTCAAGG CCTTGCAGGG CTGTGCCCAG 
CGCTTCAGGG AGCGACAGGC AGGTGGCGGT GGGGGTGTGC CCCCGGGCCC CCGGCATGTG 
CTGAGTACTC CTAACACCGT GACGAACCTG CACGTGTCTG GCACGGCAGG GCCAGAAGCT 
GCCGAGTGCC TGGTGTGCTC GGAGCTGGCA TTGCTAATTC TGTTCTCACC GTGTCAGCAC 
CGCACAGTGT GTGAGGAGTG CGCTCGAAGG ATGAAGAAGT GTATCAGGTG CCAGGTGGTC 
ATCAGCAAGA AGCTCCGCCC AGACGGCTCA GAGGTGGTAA ATGCCATCCA GGTGCCCGGC 
CCGCCTCGGC AACTGGTGGA GGAGCTACAG AGCCGCTACA GGCAGATGGA GGAGCGCATC 
ACCTGCCCCA TCTGCATCGA CAGCCACATC CGCCTGGTGT TCCAGTGCGG CCATGGAGCG 
TGCGCGCCCT GTGGCGCCGC GCTTAACGCC TGCCCCATCT GCCGCCAGCC CATCCGCGAC 
CGCATTCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109742.1
CDS	577..3417
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_030253882.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253882.1

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGCACAAC AGCTTCTGGG TGGGTGATGT GGTCCGGGTC 
ATCGGTGACC TTGACACTGT GAAGCGACTT CAGGCCGGAC ATGGTGAATG GACCGACGAC 
ATGGCTCCCG CCCTGGGCCG AGTGGGGAAA GTGGTGAAGG TGTTTGGAGA TGGAAACTTG 
CGTGTGGCGG TTGGCGGTCA GCGGTGGACC TTCAGCCCCT CTTGCCTAGT GGCCTACCGG 
CCTGAGGAAG ACGCCAACCT GGATGTGGCT GAGCGTGCCA GAGAGAACAA AAGTGCGGCA 
TCAGTCTCAG TGGCTGGTGG GAGGCAGGGC AGCCCCTGGC CAGCACTTAC CTCAACCCTG 
CCCCCAGGCT CACTGAGTGT GGCCCTGGAC AAGCTTCGGA CCCAGAAGAG TGACCCAGAG 
CACCCGGGGA GGCTGGTAGT AGAGGCCGCA CTAGGAAATG TAGCCCGGGC TCTAGATCTT 
CTGCGGAGGC ATCCAGAGCA GGTGGACACC AAGAACCAGG GTAGAACTGC CCTGCAGGTG 
GCTGCTTACC TGGGCCAGGT AGAGCTGGTG CGGCTGCTGC TGCAGGCAAG AGCAAGTATG 
GACCTGCCGG ATGATGAGGG CAACACTGTG CTGCACTACA CAGCCATGGG GAACCAGCCT 
GAGGCCACAA GGGTGCTCCT GAGTGCAGGG TGTGCAGTGG ATGCTCGGAA TGGCACACGC 
AGCACAGCCC TACATGTGGC TGTACAGAGG GGCTTCCTAG AGGTGGTAAA GATCCTGTGT 
GAACGTGGTT GTGATGTCAA CTTGCCGGAT GCGCATGCAG ACACACCCCT GCATTCTGCC 
ATCTCTGCTG GTGCTGGTGC CAGCAGCATT GTTGAAGTCC TCACCGAGGT CCCTGGCATC 
GATGTCACTG CTACCAATAG CCAGGGCTTC ACACTGCTAC ACCATGCATC CCTCAAGGGC 
CATGTGCTAG CAGTCAGGAA GATTCTGGCA AGGGCCCGGC AGCTGGTGGA TGCCAAGAAG 
GAGGATGGCT TTACCGCACT GCATCTGGCA GCCCTCAACA ACCACCGGGA GGTGGCCCAG 
GTCCTAATCC GAGAGGGTCG CTGTGATGTG AATGTGCGGA ACCGGAAGCT TCAGTCTCCA 
CTTCATCTGG CTGTACAGCA GGCCCACCTG GGGCTGGTAC CGCTGCTAGT GGACGCAGGC 
TGCAGTGTCA ACACGGAGGA CGAGGAGGGT GACACGGCCC TGCATGTGGC ACTACAGCGT 
CACCAGCTGT TGCCCCTTGT GGCTGACAGG GCTGGGGGAG ACCCAGGGCC CTTGCAGCTG 
CTGTCAAGGC TACAGGCCTC AGGCCTCCCA GGCTGCACAG AGTTGACTGT AGGCGCTGCA 
GTGGCCTGCT TCCTGGCGTT GGAGGGTGCT GATGTGAGCT ACGCAAACCA CCGCGGCCGG 
AGCCCACTGG ACCTGGCCAC AGAGGGCCGA GTGCTCAAGG CCTTGCAGGG CTGTGCCCAG 
CGCTTCAGGG AGCGACAGGC AGGTGGCGGT GGGGGTGTGC CCCCGGGCCC CCGGCATGTG 
CTGAGTACTC CTAACACCGT GACGAACCTG CACGTGTCTG GCACGGCAGG GCCAGAAGCT 
GCCGAGTGCC TGGTGTGCTC GGAGCTGGCA TTGCTAATTC TGTTCTCACC GTGTCAGCAC 
CGCACAGTGT GTGAGGAGTG CGCTCGAAGG ATGAAGAAGT GTATCAGGTG CCAGGTGGTC 
ATCAGCAAGA AGCTCCGCCC AGACGGCTCA GAGGTGGTAA ATGCCATCCA GGTGCCCGGC 
CCGCCTCGGC AACTGGTGGA GGAGCTACAG AGCCGCTACA GGCAGATGGA GGAGCGCATC 
ACCTGCCCCA TCTGCATCGA CAGCCACATC CGCCTGGTGT TCCAGTGCGG CCATGGAGCG 
TGCGCGCCCT GTGGCGCCGC GCTTAACGCC TGCCCCATCT GCCGCCAGCC CATCCGCGAC 
CGCATTCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_017320437.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175926.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OMu81120
Clone ID Related Accession (Same CDS sequence)	XM_017320437.1
Accession Version	XM_017320437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGCACC 
GCACAGTGTG TGAGGAGTGC GCTCGAAGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175926.1
CDS	855..3347
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEFIGQMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSAASVSVAGGRQGSPWPALTSTLPPGSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFSTAQCVRSALEG

Target ORF information:

RefSeq Version	XM_017320437.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320437.1

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGTTTATC GGACAGATGG GCACCGTGCA CCGCATCACA 
GACCGTGGGG ACGTGCGTGT GCAGTTCAAC CACGAGACCC GCTGGACCTT CCACCCTGGG 
GCTCTCACCA AGCACAACAG CTTCTGGGTG GGTGATGTGG TCCGGGTCAT CGGTGACCTT 
GACACTGTGA AGCGACTTCA GGCCGGACAT GGTGAATGGA CCGACGACAT GGCTCCCGCC 
CTGGGCCGAG TGGGGAAAGT GGTGAAGGTG TTTGGAGATG GAAACTTGCG TGTGGCGGTT 
GGCGGTCAGC GGTGGACCTT CAGCCCCTCT TGCCTAGTGG CCTACCGGCC TGAGGAAGAC 
GCCAACCTGG ATGTGGCTGA GCGTGCCAGA GAGAACAAAA GTGCGGCATC AGTCTCAGTG 
GCTGGTGGGA GGCAGGGCAG CCCCTGGCCA GCACTTACCT CAACCCTGCC CCCAGGCTCA 
CTGAGTGTGG CCCTGGACAA GCTTCGGACC CAGAAGAGTG ACCCAGAGCA CCCGGGGAGG 
CTGGTAGTAG AGGCCGCACT AGGAAATGTA GCCCGGGCTC TAGATCTTCT GCGGAGGCAT 
CCAGAGCAGG TGGACACCAA GAACCAGGGT AGAACTGCCC TGCAGGTGGC TGCTTACCTG 
GGCCAGGTAG AGCTGGTGCG GCTGCTGCTG CAGGCAAGAG CAAGTATGGA CCTGCCGGAT 
GATGAGGGCA ACACTGTGCT GCACTACACA GCCATGGGGA ACCAGCCTGA GGCCACAAGG 
GTGCTCCTGA GTGCAGGGTG TGCAGTGGAT GCTCGGAATG GCACACGCAG CACAGCCCTA 
CATGTGGCTG TACAGAGGGG CTTCCTAGAG GTGGTAAAGA TCCTGTGTGA ACGTGGTTGT 
GATGTCAACT TGCCGGATGC GCATGCAGAC ACACCCCTGC ATTCTGCCAT CTCTGCTGGT 
GCTGGTGCCA GCAGCATTGT TGAAGTCCTC ACCGAGGTCC CTGGCATCGA TGTCACTGCT 
ACCAATAGCC AGGGCTTCAC ACTGCTACAC CATGCATCCC TCAAGGGCCA TGTGCTAGCA 
GTCAGGAAGA TTCTGGCAAG GGCCCGGCAG CTGGTGGATG CCAAGAAGGA GGATGGCTTT 
ACCGCACTGC ATCTGGCAGC CCTCAACAAC CACCGGGAGG TGGCCCAGGT CCTAATCCGA 
GAGGGTCGCT GTGATGTGAA TGTGCGGAAC CGGAAGCTTC AGTCTCCACT TCATCTGGCT 
GTACAGCAGG CCCACCTGGG GCTGGTACCG CTGCTAGTGG ACGCAGGCTG CAGTGTCAAC 
ACGGAGGACG AGGAGGGTGA CACGGCCCTG CATGTGGCAC TACAGCGTCA CCAGCTGTTG 
CCCCTTGTGG CTGACAGGGC TGGGGGAGAC CCAGGGCCCT TGCAGCTGCT GTCAAGGCTA 
CAGGCCTCAG GCCTCCCAGG CTGCACAGAG TTGACTGTAG GCGCTGCAGT GGCCTGCTTC 
CTGGCGTTGG AGGGTGCTGA TGTGAGCTAC GCAAACCACC GCGGCCGGAG CCCACTGGAC 
CTGGCCACAG AGGGCCGAGT GCTCAAGGCC TTGCAGGGCT GTGCCCAGCG CTTCAGCACC 
GCACAGTGTG TGAGGAGTGC GCTCGAAGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_017320438.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_017175927.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OMu81121
Clone ID Related Accession (Same CDS sequence)	XM_017320438.1
Accession Version	XM_017320438.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2406bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGATGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAACCA CGAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG 
CACAACAGCT TCTGGGTGGG TGATGTGGTC CGGGTCATCG GTGACCTTGA CACTGTGAAG 
CGACTTCAGG CCGGACATGG TGAATGGACC GACGACATGG CTCCCGCCCT GGGCCGAGTG 
GGGAAAGTGG TGAAGGTGTT TGGAGATGGA AACTTGCGTG TGGCGGTTGG CGGTCAGCGG 
TGGACCTTCA GCCCCTCTTG CCTAGTGGCC TACCGGCCTG AGGAAGACGC CAACCTGGAT 
GTGGCTGAGC GTGCCAGAGA GAACAAAAGC TCACTGAGTG TGGCCCTGGA CAAGCTTCGG 
ACCCAGAAGA GTGACCCAGA GCACCCGGGG AGGCTGGTAG TAGAGGCCGC ACTAGGAAAT 
GTAGCCCGGG CTCTAGATCT TCTGCGGAGG CATCCAGAGC AGGTGGACAC CAAGAACCAG 
GGTAGAACTG CCCTGCAGGT GGCTGCTTAC CTGGGCCAGG TAGAGCTGGT GCGGCTGCTG 
CTGCAGGCAA GAGCAAGTAT GGACCTGCCG GATGATGAGG GCAACACTGT GCTGCACTAC 
ACAGCCATGG GGAACCAGCC TGAGGCCACA AGGGTGCTCC TGAGTGCAGG GTGTGCAGTG 
GATGCTCGGA ATGGCACACG CAGCACAGCC CTACATGTGG CTGTACAGAG GGGCTTCCTA 
GAGGTGGTAA AGATCCTGTG TGAACGTGGT TGTGATGTCA ACTTGCCGGA TGCGCATGCA 
GACACACCCC TGCATTCTGC CATCTCTGCT GGTGCTGGTG CCAGCAGCAT TGTTGAAGTC 
CTCACCGAGG TCCCTGGCAT CGATGTCACT GCTACCAATA GCCAGGGCTT CACACTGCTA 
CACCATGCAT CCCTCAAGGG CCATGTGCTA GCAGTCAGGA AGATTCTGGC AAGGGCCCGG 
CAGCTGGTGG ATGCCAAGAA GGAGGATGGC TTTACCGCAC TGCATCTGGC AGCCCTCAAC 
AACCACCGGG AGGTGGCCCA GGTCCTAATC CGAGAGGGTC GCTGTGATGT GAATGTGCGG 
AACCGGAAGC TTCAGTCTCC ACTTCATCTG GCTGTACAGC AGGCCCACCT GGGGCTGGTA 
CCGCTGCTAG TGGACGCAGG CTGCAGTGTC AACACGGAGG ACGAGGAGGG TGACACGGCC 
CTGCATGTGG CACTACAGCG TCACCAGCTG TTGCCCCTTG TGGCTGACAG GGCTGGGGGA 
GACCCAGGGC CCTTGCAGCT GCTGTCAAGG CTACAGGCCT CAGGCCTCCC AGGCTGCACA 
GAGTTGACTG TAGGCGCTGC AGTGGCCTGC TTCCTGGCGT TGGAGGGTGC TGATGTGAGC 
TACGCAAACC ACCGCGGCCG GAGCCCACTG GACCTGGCCA CAGAGGGCCG AGTGCTCAAG 
GCCTTGCAGG GCTGTGCCCA GCGCTTCAGC ACCGCACAGT GTGTGAGGAG TGCGCTCGAA 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017175927.1
CDS	855..3260
Translation	MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFSTAQCVRSALEG

Target ORF information:

RefSeq Version	XM_017320438.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017320438.1

ORF Insert Sequence:

ATGGACCTGG ACCCCCATGC AGGTGTGCAG GTGGGCATGC GTGTGGTACG CGGAATGGAC 
TGGAAATGGG GCCAGCAGGA TGGAGGTGAG GGCGGTGTGG GCACCGTGGT GGAGCTTGGC 
CGCCATGGCA GCCCCTCGAC CCCCGACCGT ACAGTTGTCG TTCAGTGGGA CCAGGGCACA 
CGTACCAACT ATCGAGCTGG CTACCAAGGT GCCCATGACC TGTTGCTCTA TGACAACGCC 
CAAATCGGTA TCCGCCACCC CAATATCATC TGTGACTGCT GCAAGAAACA TGGGCTTCGG 
GGCATGCGTT GGAAGTGCCG CGTCTGCTTT GACTATGACC TCTGCACACA GTGCTACATG 
CACAACAAGC ATGACCTGAC CCATGCCTTC GAGCGCTATG AGACATCTCA CTCACGCCCG 
GTTACGCTGA GCCCCCGACA GGGCCTCCCT CGGATCCCAC TGAGGGGCAT CTTTCAAGGA 
GCTAAGGTGG TACGAGGCCC TGACTGGGAA TGGGGCTCAC AAGATGGAGG GGAAGGCAAG 
ACAGGTCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGAAG TGTGGCCAGT 
GTGACGTGGG CAGATGGAAC CACGAACGTG TACCGAGTGG GCCACAAGGG CAAGGTGGAT 
CTCAGATGTG TTGGTGAGGC AGCTGGCGGC TTTTACTACA AGGAGCACCT CCCAAAGCTT 
GGCAAGCCAG CAGAGCTGCA GCGCAGGGTG AGTGCTGATG GCCAGCCCTT CCAGCGTGGG 
GACAAGGTCA AGTGTCTGCT GGACACAGAT GTCCTAAGGG ATATGCAAGA AGGCCATGGT 
GGCTGGAACC CTAGGATGGC AGAGATGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC 
GTGCGTGTGC AGTTCAACCA CGAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG 
CACAACAGCT TCTGGGTGGG TGATGTGGTC CGGGTCATCG GTGACCTTGA CACTGTGAAG 
CGACTTCAGG CCGGACATGG TGAATGGACC GACGACATGG CTCCCGCCCT GGGCCGAGTG 
GGGAAAGTGG TGAAGGTGTT TGGAGATGGA AACTTGCGTG TGGCGGTTGG CGGTCAGCGG 
TGGACCTTCA GCCCCTCTTG CCTAGTGGCC TACCGGCCTG AGGAAGACGC CAACCTGGAT 
GTGGCTGAGC GTGCCAGAGA GAACAAAAGC TCACTGAGTG TGGCCCTGGA CAAGCTTCGG 
ACCCAGAAGA GTGACCCAGA GCACCCGGGG AGGCTGGTAG TAGAGGCCGC ACTAGGAAAT 
GTAGCCCGGG CTCTAGATCT TCTGCGGAGG CATCCAGAGC AGGTGGACAC CAAGAACCAG 
GGTAGAACTG CCCTGCAGGT GGCTGCTTAC CTGGGCCAGG TAGAGCTGGT GCGGCTGCTG 
CTGCAGGCAA GAGCAAGTAT GGACCTGCCG GATGATGAGG GCAACACTGT GCTGCACTAC 
ACAGCCATGG GGAACCAGCC TGAGGCCACA AGGGTGCTCC TGAGTGCAGG GTGTGCAGTG 
GATGCTCGGA ATGGCACACG CAGCACAGCC CTACATGTGG CTGTACAGAG GGGCTTCCTA 
GAGGTGGTAA AGATCCTGTG TGAACGTGGT TGTGATGTCA ACTTGCCGGA TGCGCATGCA 
GACACACCCC TGCATTCTGC CATCTCTGCT GGTGCTGGTG CCAGCAGCAT TGTTGAAGTC 
CTCACCGAGG TCCCTGGCAT CGATGTCACT GCTACCAATA GCCAGGGCTT CACACTGCTA 
CACCATGCAT CCCTCAAGGG CCATGTGCTA GCAGTCAGGA AGATTCTGGC AAGGGCCCGG 
CAGCTGGTGG ATGCCAAGAA GGAGGATGGC TTTACCGCAC TGCATCTGGC AGCCCTCAAC 
AACCACCGGG AGGTGGCCCA GGTCCTAATC CGAGAGGGTC GCTGTGATGT GAATGTGCGG 
AACCGGAAGC TTCAGTCTCC ACTTCATCTG GCTGTACAGC AGGCCCACCT GGGGCTGGTA 
CCGCTGCTAG TGGACGCAGG CTGCAGTGTC AACACGGAGG ACGAGGAGGG TGACACGGCC 
CTGCATGTGG CACTACAGCG TCACCAGCTG TTGCCCCTTG TGGCTGACAG GGCTGGGGGA 
GACCCAGGGC CCTTGCAGCT GCTGTCAAGG CTACAGGCCT CAGGCCTCCC AGGCTGCACA 
GAGTTGACTG TAGGCGCTGC AGTGGCCTGC TTCCTGGCGT TGGAGGGTGC TGATGTGAGC 
TACGCAAACC ACCGCGGCCG GAGCCCACTG GACCTGGCCA CAGAGGGCCG AGTGCTCAAG 
GCCTTGCAGG GCTGTGCCCA GCGCTTCAGC ACCGCACAGT GTGTGAGGAG TGCGCTCGAA 
GGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Mib2 ( XM_030253883.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_030109743.1 Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OMu88645
Clone ID Related Accession (Same CDS sequence)	XM_030253883.1
Accession Version	XM_030253883.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	E3 ubiquitin-protein ligase MIB2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000070.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGTTGGA AGTGCCGCGT CTGCTTTGAC TATGACCTCT GCACACAGTG CTACATGCAC 
AACAAGCATG ACCTGACCCA TGCCTTCGAG CGCTATGAGA CATCTCACTC ACGCCCGGTT 
ACGCTGAGCC CCCGACAGGG CCTCCCTCGG ATCCCACTGA GGGGCATCTT TCAAGGAGCT 
AAGGTGGTAC GAGGCCCTGA CTGGGAATGG GGCTCACAAG ATGGAGGGGA AGGCAAGACA 
GGTCGTGTGG TGGACATCCG TGGCTGGGAT GTGGAGACAG GCCGAAGTGT GGCCAGTGTG 
ACGTGGGCAG ATGGAACCAC GAACGTGTAC CGAGTGGGCC ACAAGGGCAA GGTGGATCTC 
AGATGTGTTG GTGAGGCAGC TGGCGGCTTT TACTACAAGG AGCACCTCCC AAAGCTTGGC 
AAGCCAGCAG AGCTGCAGCG CAGGGTGAGT GCTGATGGCC AGCCCTTCCA GCGTGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACAGATGTC CTAAGGGATA TGCAAGAAGG CCATGGTGGC 
TGGAACCCTA GGATGGCAGA GCACAACAGC TTCTGGGTGG GTGATGTGGT CCGGGTCATC 
GGTGACCTTG ACACTGTGAA GCGACTTCAG GCCGGACATG GTGAATGGAC CGACGACATG 
GCTCCCGCCC TGGGCCGAGT GGGGAAAGTG GTGAAGGTGT TTGGAGATGG AAACTTGCGT 
GTGGCGGTTG GCGGTCAGCG GTGGACCTTC AGCCCCTCTT GCCTAGTGGC CTACCGGCCT 
GAGGAAGACG CCAACCTGGA TGTGGCTGAG CGTGCCAGAG AGAACAAAAG CTCACTGAGT 
GTGGCCCTGG ACAAGCTTCG GACCCAGAAG AGTGACCCAG AGCACCCGGG GAGGCTGGTA 
GTAGAGGCCG CACTAGGAAA TGTAGCCCGG GCTCTAGATC TTCTGCGGAG GCATCCAGAG 
CAGGTGGACA CCAAGAACCA GGGTAGAACT GCCCTGCAGG TGGCTGCTTA CCTGGGCCAG 
GTAGAGCTGG TGCGGCTGCT GCTGCAGGCA AGAGCAAGTA TGGACCTGCC GGATGATGAG 
GGCAACACTG TGCTGCACTA CACAGCCATG GGGAACCAGC CTGAGGCCAC AAGGGTGCTC 
CTGAGTGCAG GGTGTGCAGT GGATGCTCGG AATGGCACAC GCAGCACAGC CCTACATGTG 
GCTGTACAGA GGGGCTTCCT AGAGGTGGTA AAGATCCTGT GTGAACGTGG TTGTGATGTC 
AACTTGCCGG ATGCGCATGC AGACACACCC CTGCATTCTG CCATCTCTGC TGGTGCTGGT 
GCCAGCAGCA TTGTTGAAGT CCTCACCGAG GTCCCTGGCA TCGATGTCAC TGCTACCAAT 
AGCCAGGGCT TCACACTGCT ACACCATGCA TCCCTCAAGG GCCATGTGCT AGCAGTCAGG 
AAGATTCTGG CAAGGGCCCG GCAGCTGGTG GATGCCAAGA AGGAGGATGG CTTTACCGCA 
CTGCATCTGG CAGCCCTCAA CAACCACCGG GAGGTGGCCC AGGTCCTAAT CCGAGAGGGT 
CGCTGTGATG TGAATGTGCG GAACCGGAAG CTTCAGTCTC CACTTCATCT GGCTGTACAG 
CAGGCCCACC TGGGGCTGGT ACCGCTGCTA GTGGACGCAG GCTGCAGTGT CAACACGGAG 
GACGAGGAGG GTGACACGGC CCTGCATGTG GCACTACAGC GTCACCAGCT GTTGCCCCTT 
GTGGCTGACA GGGCTGGGGG AGACCCAGGG CCCTTGCAGC TGCTGTCAAG GCTACAGGCC 
TCAGGCCTCC CAGGCTGCAC AGAGTTGACT GTAGGCGCTG CAGTGGCCTG CTTCCTGGCG 
TTGGAGGGTG CTGATGTGAG CTACGCAAAC CACCGCGGCC GGAGCCCACT GGACCTGGCC 
ACAGAGGGCC GAGTGCTCAA GGCCTTGCAG GGCTGTGCCC AGCGCTTCAG GGAGCGACAG 
GCAGGTGGCG GTGGGGGTGT GCCCCCGGGC CCCCGGCATG TGCTGAGTAC TCCTAACACC 
GTGACGAACC TGCACGTGTC TGGCACGGCA GGGCCAGAAG CTGCCGAGTG CCTGGTGTGC 
TCGGAGCTGG CATTGCTAAT TCTGTTCTCA CCGTGTCAGC ACCGCACAGT GTGTGAGGAG 
TGCGCTCGAA GGATGAAGAA GTGTATCAGG TGCCAGGTGG TCATCAGCAA GAAGCTCCGC 
CCAGACGGCT CAGAGGTGGT AAATGCCATC CAGGTGCCCG GCCCGCCTCG GCAACTGGTG 
GAGGAGCTAC AGAGCCGCTA CAGGCAGATG GAGGAGCGCA TCACCTGCCC CATCTGCATC 
GACAGCCACA TCCGCCTGGT GTTCCAGTGC GGCCATGGAG CGTGCGCGCC CTGTGGCGCC 
GCGCTTAACG CCTGCCCCAT CTGCCGCCAG CCCATCCGCG ACCGCATTCA GATCTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_030109743.1
CDS	941..3403
Translation	MRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHGGWNPRMAEHNSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRTQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLPDDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHADTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTALHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPLDLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLILFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_030253883.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus mindbomb E3 ubiquitin protein ligase 2 (Mib2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_030253883.1

ORF Insert Sequence:

ATGCGTTGGA AGTGCCGCGT CTGCTTTGAC TATGACCTCT GCACACAGTG CTACATGCAC 
AACAAGCATG ACCTGACCCA TGCCTTCGAG CGCTATGAGA CATCTCACTC ACGCCCGGTT 
ACGCTGAGCC CCCGACAGGG CCTCCCTCGG ATCCCACTGA GGGGCATCTT TCAAGGAGCT 
AAGGTGGTAC GAGGCCCTGA CTGGGAATGG GGCTCACAAG ATGGAGGGGA AGGCAAGACA 
GGTCGTGTGG TGGACATCCG TGGCTGGGAT GTGGAGACAG GCCGAAGTGT GGCCAGTGTG 
ACGTGGGCAG ATGGAACCAC GAACGTGTAC CGAGTGGGCC ACAAGGGCAA GGTGGATCTC 
AGATGTGTTG GTGAGGCAGC TGGCGGCTTT TACTACAAGG AGCACCTCCC AAAGCTTGGC 
AAGCCAGCAG AGCTGCAGCG CAGGGTGAGT GCTGATGGCC AGCCCTTCCA GCGTGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACAGATGTC CTAAGGGATA TGCAAGAAGG CCATGGTGGC 
TGGAACCCTA GGATGGCAGA GCACAACAGC TTCTGGGTGG GTGATGTGGT CCGGGTCATC 
GGTGACCTTG ACACTGTGAA GCGACTTCAG GCCGGACATG GTGAATGGAC CGACGACATG 
GCTCCCGCCC TGGGCCGAGT GGGGAAAGTG GTGAAGGTGT TTGGAGATGG AAACTTGCGT 
GTGGCGGTTG GCGGTCAGCG GTGGACCTTC AGCCCCTCTT GCCTAGTGGC CTACCGGCCT 
GAGGAAGACG CCAACCTGGA TGTGGCTGAG CGTGCCAGAG AGAACAAAAG CTCACTGAGT 
GTGGCCCTGG ACAAGCTTCG GACCCAGAAG AGTGACCCAG AGCACCCGGG GAGGCTGGTA 
GTAGAGGCCG CACTAGGAAA TGTAGCCCGG GCTCTAGATC TTCTGCGGAG GCATCCAGAG 
CAGGTGGACA CCAAGAACCA GGGTAGAACT GCCCTGCAGG TGGCTGCTTA CCTGGGCCAG 
GTAGAGCTGG TGCGGCTGCT GCTGCAGGCA AGAGCAAGTA TGGACCTGCC GGATGATGAG 
GGCAACACTG TGCTGCACTA CACAGCCATG GGGAACCAGC CTGAGGCCAC AAGGGTGCTC 
CTGAGTGCAG GGTGTGCAGT GGATGCTCGG AATGGCACAC GCAGCACAGC CCTACATGTG 
GCTGTACAGA GGGGCTTCCT AGAGGTGGTA AAGATCCTGT GTGAACGTGG TTGTGATGTC 
AACTTGCCGG ATGCGCATGC AGACACACCC CTGCATTCTG CCATCTCTGC TGGTGCTGGT 
GCCAGCAGCA TTGTTGAAGT CCTCACCGAG GTCCCTGGCA TCGATGTCAC TGCTACCAAT 
AGCCAGGGCT TCACACTGCT ACACCATGCA TCCCTCAAGG GCCATGTGCT AGCAGTCAGG 
AAGATTCTGG CAAGGGCCCG GCAGCTGGTG GATGCCAAGA AGGAGGATGG CTTTACCGCA 
CTGCATCTGG CAGCCCTCAA CAACCACCGG GAGGTGGCCC AGGTCCTAAT CCGAGAGGGT 
CGCTGTGATG TGAATGTGCG GAACCGGAAG CTTCAGTCTC CACTTCATCT GGCTGTACAG 
CAGGCCCACC TGGGGCTGGT ACCGCTGCTA GTGGACGCAG GCTGCAGTGT CAACACGGAG 
GACGAGGAGG GTGACACGGC CCTGCATGTG GCACTACAGC GTCACCAGCT GTTGCCCCTT 
GTGGCTGACA GGGCTGGGGG AGACCCAGGG CCCTTGCAGC TGCTGTCAAG GCTACAGGCC 
TCAGGCCTCC CAGGCTGCAC AGAGTTGACT GTAGGCGCTG CAGTGGCCTG CTTCCTGGCG 
TTGGAGGGTG CTGATGTGAG CTACGCAAAC CACCGCGGCC GGAGCCCACT GGACCTGGCC 
ACAGAGGGCC GAGTGCTCAA GGCCTTGCAG GGCTGTGCCC AGCGCTTCAG GGAGCGACAG 
GCAGGTGGCG GTGGGGGTGT GCCCCCGGGC CCCCGGCATG TGCTGAGTAC TCCTAACACC 
GTGACGAACC TGCACGTGTC TGGCACGGCA GGGCCAGAAG CTGCCGAGTG CCTGGTGTGC 
TCGGAGCTGG CATTGCTAAT TCTGTTCTCA CCGTGTCAGC ACCGCACAGT GTGTGAGGAG 
TGCGCTCGAA GGATGAAGAA GTGTATCAGG TGCCAGGTGG TCATCAGCAA GAAGCTCCGC 
CCAGACGGCT CAGAGGTGGT AAATGCCATC CAGGTGCCCG GCCCGCCTCG GCAACTGGTG 
GAGGAGCTAC AGAGCCGCTA CAGGCAGATG GAGGAGCGCA TCACCTGCCC CATCTGCATC 
GACAGCCACA TCCGCCTGGT GTTCCAGTGC GGCCATGGAG CGTGCGCGCC CTGTGGCGCC 
GCGCTTAACG CCTGCCCCAT CTGCCGCCAG CCCATCCGCG ACCGCATTCA GATCTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A high-resolution spatiotemporal atlas of gene expression of the developing mouse brain.
Neuron83(2)309-323(2014 Jul)
Thompson CL,Ng L,Menon V,Martinez S,Lee CK,Glattfelder K,Sunkin SM,Henry A,Lau C,Dang C,Garcia-Lopez R,Martinez-Ferre A,Pombero A,Rubenstein JLR,Wakeman WB,Hohmann J,Dee N,Sodt AJ,Young R,Smith K,Nguyen TN,Kidney J,Kuan L,Jeromin A,Kaykas A,Miller J,Page D,Orta G,Bernard A,Riley Z,Smith S,Wohnoutka P,Hawrylycz MJ,Puelles L,Jones AR

The E3 ubiquitin ligase mind bomb-2 (MIB2) protein controls B-cell CLL/lymphoma 10 (BCL10)-dependent NF-?B activation.
The Journal of biological chemistry286(43)37147-57(2011 Oct)
Stempin CC,Chi L,Giraldo-Vela JP,High AA,H?cker H,Redecke V

Mapping a dynamic innate immunity protein interaction network regulating type I interferon production.
Immunity35(3)426-40(2011 Sep)
Li S,Wang L,Berman M,Kong YY,Dorf ME

A systems approach reveals that the myogenesis genome network is regulated by the transcriptional repressor RP58.
Developmental cell17(6)836-48(2009 Dec)
Yokoyama S,Ito Y,Ueno-Kudoh H,Shimizu H,Uchibe K,Albini S,Mitsuoka K,Miyaki S,Kiso M,Nagai A,Hikata T,Osada T,Fukuda N,Yamashita S,Harada D,Mezzano V,Kasai M,Puri PL,Hayashizaki Y,Okado H,Hashimoto M,Asahara H

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Mib2 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

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