MIB2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MIB2
Entrez Gene ID	142678
Full Name	mindbomb E3 ubiquitin protein ligase 2
Synonyms	ZZANK1,ZZZ5
General protein information	Preferred Names mindbomb E3 ubiquitin protein ligase 2 Names E3 ubiquitin-protein ligase MIB2 RING-type E3 ubiquitin transferase MIB2 mind bomb homolog 2 mindbomb homolog 2 novel zinc finger protein novelzin putative NF-kappa-B-activating protein 002N skeletrophin zinc finger ZZ type with ankyrin repeat domain protein 1 zinc finger, ZZ type with ankyrin repeat domain 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.33
Summary	The protein encoded by this gene is an E3 ubiquitin protein ligase that mediates ubiquitination of proteins in the Notch signaling pathway. The encoded protein may be a suppressor of melanoma invasion. [provided by RefSeq, Mar 2017].
Disorder MIM:	611141

mRNA and Protein(s)

mRNA	Protein	Name
XM_017000357.1	XP_016855846.1	E3 ubiquitin-protein ligase MIB2 isoform X11
XM_011540731.2	XP_011539033.1	E3 ubiquitin-protein ligase MIB2 isoform X6
XM_017000349.2	XP_016855838.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_017000351.2	XP_016855840.1	E3 ubiquitin-protein ligase MIB2 isoform X3
XM_017000354.2	XP_016855843.1	E3 ubiquitin-protein ligase MIB2 isoform X7
XM_017000355.2	XP_016855844.1	E3 ubiquitin-protein ligase MIB2 isoform X8
XM_011540737.3	XP_011539039.1	E3 ubiquitin-protein ligase MIB2 isoform X12
XM_017000362.1	XP_016855851.1	E3 ubiquitin-protein ligase MIB2 isoform X19
XM_006710372.1	XP_006710435.1	E3 ubiquitin-protein ligase MIB2 isoform X21
XM_024453319.1	XP_024309087.1	E3 ubiquitin-protein ligase MIB2 isoform X24
NM_001170689.2	NP_001164160.1	E3 ubiquitin-protein ligase MIB2 isoform 5
NM_001170688.1	NP_001164159.1	E3 ubiquitin-protein ligase MIB2 isoform 4
NM_001170686.2	NP_001164157.2	E3 ubiquitin-protein ligase MIB2 isoform 2
XM_017000352.1	XP_016855841.1	E3 ubiquitin-protein ligase MIB2 isoform X4
XM_011540741.2	XP_011539043.1	E3 ubiquitin-protein ligase MIB2 isoform X13
XM_011540737.2	XP_011539039.1	E3 ubiquitin-protein ligase MIB2 isoform X12
NM_001170689.1	NP_001164160.1	E3 ubiquitin-protein ligase MIB2 isoform 5
NM_001170686.1	NP_001164157.1	E3 ubiquitin-protein ligase MIB2 isoform 2
NM_080875.3	NP_543151.3	E3 ubiquitin-protein ligase MIB2 isoform 1
NM_001170687.2	NP_001164158.2	E3 ubiquitin-protein ligase MIB2 isoform 3
XM_011540731.3	XP_011539033.1	E3 ubiquitin-protein ligase MIB2 isoform X6
XM_024453317.1	XP_024309085.1	E3 ubiquitin-protein ligase MIB2 isoform X19
XM_017000363.1	XP_016855852.1	E3 ubiquitin-protein ligase MIB2 isoform X22
XM_017000364.1	XP_016855853.1	E3 ubiquitin-protein ligase MIB2 isoform X23
XM_017000365.1	XP_016855854.1	E3 ubiquitin-protein ligase MIB2 isoform X25
XM_017000351.1	XP_016855840.1	E3 ubiquitin-protein ligase MIB2 isoform X3
NM_080875.2	NP_543151.2	E3 ubiquitin-protein ligase MIB2 isoform 1
XM_017000354.1	XP_016855843.1	E3 ubiquitin-protein ligase MIB2 isoform X7
XM_017000356.1	XP_016855845.1	E3 ubiquitin-protein ligase MIB2 isoform X10
XM_017000353.1	XP_016855842.1	E3 ubiquitin-protein ligase MIB2 isoform X5
XM_017000352.2	XP_016855841.1	E3 ubiquitin-protein ligase MIB2 isoform X4
XM_017000353.2	XP_016855842.1	E3 ubiquitin-protein ligase MIB2 isoform X5
XM_011540736.3	XP_011539038.1	E3 ubiquitin-protein ligase MIB2 isoform X9
XM_017000356.2	XP_016855845.1	E3 ubiquitin-protein ligase MIB2 isoform X10
XM_017000358.1	XP_016855847.1	E3 ubiquitin-protein ligase MIB2 isoform X15
XM_017000359.1	XP_016855848.1	E3 ubiquitin-protein ligase MIB2 isoform X16
XM_017000361.1	XP_016855850.1	E3 ubiquitin-protein ligase MIB2 isoform X18
XM_011540736.2	XP_011539038.1	E3 ubiquitin-protein ligase MIB2 isoform X9
XM_011540742.2	XP_011539044.1	E3 ubiquitin-protein ligase MIB2 isoform X14
XM_017000350.1	XP_016855839.1	E3 ubiquitin-protein ligase MIB2 isoform X2
XM_011540741.3	XP_011539043.1	E3 ubiquitin-protein ligase MIB2 isoform X13
XM_011540742.3	XP_011539044.1	E3 ubiquitin-protein ligase MIB2 isoform X14
XM_017000360.1	XP_016855849.1	E3 ubiquitin-protein ligase MIB2 isoform X17
XM_017000366.1	XP_016855855.1	E3 ubiquitin-protein ligase MIB2 isoform X26
NM_001170687.1	NP_001164158.1	E3 ubiquitin-protein ligase MIB2 isoform 3
XM_017000349.1	XP_016855838.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_017000355.1	XP_016855844.1	E3 ubiquitin-protein ligase MIB2 isoform X8
XM_017000350.2	XP_016855839.1	E3 ubiquitin-protein ligase MIB2 isoform X2
XM_017000357.2	XP_016855846.1	E3 ubiquitin-protein ligase MIB2 isoform X11
XM_024453318.1	XP_024309086.1	E3 ubiquitin-protein ligase MIB2 isoform X20

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mib2	NP_001005901.2
Xenopus tropicalis (tropical clawed frog)	mib2	NP_001116916.1
Homo sapiens (human)	MIB2	NP_543151.2
Bos taurus (cattle)	MIB2	XP_002694171.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008851	XP_319593.4
Gallus gallus (chicken)	MIB2	NP_001006301.1
Drosophila melanogaster (fruit fly)	mib2	NP_001260554.1
Pan troglodytes (chimpanzee)	MIB2	XP_001155032.3
Mus musculus (house mouse)	Mib2	NP_001243036.1
	MIB2	XP_001096926.2
Canis lupus familiaris (dog)	MIB2	XP_005620446.1
Danio rerio (zebrafish)	mib2	NP_001073146.1

Gene Ontology
Bibliography

Related articles in PubMed

MIB2 variants altering NOTCH signalling result in left ventricle hypertrabeculation/non-compaction and are associated with M?n?trier-like gastropathy.
Piccolo P, Attanasio S, Secco I, Sangermano R, Strisciuglio C, Limongelli G, Miele E, Mutarelli M, Banfi S, Nigro V, Pons T, Valencia A, Zentilin L, Campione S, Nardone G, Lynnes TC, Celestino-Soper PB, Spoonamore KG, D'Armiento FP, Giacca M, Staiano A, Vatta M, Collesi C, Brunetti-Pierri N
Human molecular genetics26(1)33-43(2017 01)

The cylindromatosis gene product, CYLD, interacts with MIB2 to regulate notch signalling.
Rajan N, Elliott RJ, Smith A, Sinclair N, Swift S, Lord CJ, Ashworth A
Oncotarget5(23)12126-40(2014 Dec)

Lysine 63-linked TANK-binding kinase 1 ubiquitination by mindbomb E3 ubiquitin protein ligase 2 is mediated by the mitochondrial antiviral signaling protein.
Ye JS, Kim N, Lee KJ, Nam YR, Lee U, Joo CH
Journal of virology88(21)12765-76(2014 Nov)

The E3 ubiquitin ligase mind bomb-2 (MIB2) protein controls B-cell CLL/lymphoma 10 (BCL10)-dependent NF-?B activation.
Stempin CC, Chi L, Giraldo-Vela JP, High AA, H?cker H, Redecke V
The Journal of biological chemistry286(43)37147-57(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Missense p.V742G variant in MIB2 is associated with left ventricle hypertrabeculation/non-compaction and Menetrier-like gastropathy.
Title: MIB2 variants altering NOTCH signalling result in left ventricle hypertrabeculation/non-compaction and are associated with M?n?trier-like gastropathy.

Here, we report a novel interaction between CYLD and MIB2 that supports the hypothesis that the CYLD/MIB2 interaction might play a pathogenic role in human cancer.
Title: The cylindromatosis gene product, CYLD, interacts with MIB2 to regulate notch signalling.

The DLAIS motif in MAVS was found to be critical for MIB2 binding, the ligation of K63-linked ubiquitin to TANK-binding kinase 1, and phosphorylation-mediated IRF3/7 activation.
Title: Lysine 63-linked TANK-binding kinase 1 ubiquitination by mindbomb E3 ubiquitin protein ligase 2 is mediated by the mitochondrial antiviral signaling protein.

identify MIB2 as a novel component of the activated BCL10 signaling complex and a missing link in the BCL10-dependent NF-kappaB signaling pathway.
Title: The E3 ubiquitin ligase mind bomb-2 (MIB2) protein controls B-cell CLL/lymphoma 10 (BCL10)-dependent NF-?B activation.

Data identify Mind bomb-2 as an E3 ubiquitin ligase that interacts with and ubiquitinates the NR2B subunit of the NMDAR in mammalian cells.
Title: Mind bomb-2 is an E3 ligase that ubiquitinates the N-methyl-D-aspartate receptor NR2B subunit in a phosphorylation-dependent manner.

The following MIB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12048	NM_001170689.2 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00
OHu107540	NM_001170687.2 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu107534	NM_001170686.2 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu107536	NM_080875.3 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu103597	XM_024453318.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu103598	XM_024453319.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu01261	NM_001170688.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OHu01520	NM_001170687.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$741.30 $1059.00
OHu01762	NM_001170686.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$755.30 $1079.00
OHu01981	NM_080875.2 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$755.30 $1079.00
OHu33040	XM_006710372.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu75979	XM_011540731.3 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu75984	XM_011540736.3 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu75985	XM_011540737.3 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu75989	XM_011540741.3 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu75990	XM_011540742.3 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83158	XM_017000349.2 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83159	XM_017000350.2 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83160	XM_017000351.2 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83161	XM_017000352.2 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83162	XM_017000353.2 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83163	XM_017000354.2 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83164	XM_017000355.2 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83165	XM_017000356.2 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83166	XM_017000357.2 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83167	XM_017000358.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu83168	XM_017000359.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu83169	XM_017000360.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu83170	XM_017000361.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu83171	XM_024453317.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu83171	XM_017000362.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu83172	XM_017000363.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu83173	XM_017000364.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$559.30 $799.00
OHu83174	XM_017000365.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu83175	XM_017000366.1 Latest version!	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu75985	XM_011540737.2	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu12048	NM_001170689.1	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00
OHu75979	XM_011540731.2	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83160	XM_017000351.1	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83163	XM_017000354.1	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83161	XM_017000352.1	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83162	XM_017000353.1	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83165	XM_017000356.1	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu75984	XM_011540736.2	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu75989	XM_011540741.2	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu75990	XM_011540742.2	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83158	XM_017000349.1	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83164	XM_017000355.1	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83159	XM_017000350.1	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu83166	XM_017000357.1	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIB2 ( NM_001170689.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001164160.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 5, mRNA.

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CloneID	OHu12048
Clone ID Related Accession (Same CDS sequence)	NM_001170689.2 , NM_001170689.1
Accession Version	NM_001170689.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL691432.54 and FO704657.2. On Dec 21, 2018 this sequence version replaced NM_001170689.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK128167.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2157511 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCAG ACCCCCAGGC GGGCGTGCAG GTGGGCATGC GGGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTTGGC 
CGCCACGGCA GCCCCTCGAC ACCCGACCGC ACAGTGGTCG TGCAGTGGGA CCAGGGCACG 
CGCACCAACT ACCGCGCCGG CTACCAGGGC GCGCACGACC TGCTGCTGTA CGACAACGCC 
CAGATCGGCG TCCGGCACCC CAACATCATC TGTGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGGATGCGCT GGAAGTGCCG TGTGTGCCTG GACTACGACC TCTGCACGCA GTGCTACATG 
CACAACAAGC ATGAGCTCGC CCACGCCTTC GACCGCTACG AGACCGCTCA CTCGCGCCCT 
GTCACACTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAC TAAGGGGCAT CTTCCAGGGA 
GCGAAGGTGG TGCGAGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGT TTATCGGACA GACGGGCACC GTGCATCGTA TCACGGACCG CGGGGACGTG 
CGCGTGCAGT TCAACCACGA GACGCGCTGG ACCTTCCACC CCGGGGCGCT CACCAAGCAC 
CACTCCTTCT GGGTGGGCGA CGTGGTCCGG GTCATCGGCG ACCTTGACAC AGTGAAGCGG 
CTGCAGGCTG GGCATGGCGA GTGGACGGAC GACATGGCCC CTGCCCTGGG CCGCGTCGGG 
AAGGTGGTGA AAGTGTTTGG AGACGGGAAC CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG 
ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC CGGCCCGAGG AGGATGCCAA CCTGGACGTG 
GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA CTGAGCGTGG CCCTGGACAA GCTTCGGGCC 
CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG CTGGTGGTGG AGGTGGCGCT GGGTAACGCA 
GCCCGGGCTC TGGACCTGCT GCGGAGGCGC CCAGAGCAGG TGGACACCAA GAACCAAGGC 
AGGACCGCTC TGCAAGTGGC TGCCTACCTG GGCCAGGTGG AGTTGATACG GCTGCTGCTA 
CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC GACGAGGGCA ACACGGCACT GCACTACGCG 
GCCCTGGGGA ACCAGCCCGA GGCCACCAGG GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC 
GCCATCAACA GCACCCAGAG CACAGCACTG CACGTGGCCG TGCAGAGGGG CTTCCTGGAG 
GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT GACGTCAACC TGCCCGACGC CCACTCGGAC 
ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC ACTGGAGCCA GCGGCATTGT CGAGGTCCTC 
ACGGAGGTGC CAAACATCGA TGTTACCGCC ACCAACAGCC AGGGTTTCAC CCTGCTGCAC 
CATGCCTCCC TCAAGGGTCA CGCGCTAGCT GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG 
CTGGTGGACG CCAAGAAGGA GGACGGCTTC ACGGCGCTGC ATCTGGCTGC CCTCAACAAC 
CACCGCGAGG TGGCCCAGAT CCTCATCCGG GAGGGCCGCT GTGACGTGAA CGTGCGCAAC 
CGGAAGCTGC AGTCCCCGCT GCATCTCGCC GTGCAACAGG CCCACGTGGG GCTGGTGCCG 
CTACTGGTGG ACGCTGGGTG CAGTGTCAAC GCCGAGGACG AGGAGGGGGA CACAGCCCTG 
CACGTGGCGC TGCAGCGTCA TCAGCTGCTG CCCCTGGTGG CTGATGGGGC CGGGGGGGAC 
CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG 
CTGACGGTGG GCGCGGCGGT CGCCTGCTTC CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC 
ACCAACCACC GCGGTCGGAG CCCGCTGGAC CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC 
CTTCAGGGCT GCGCCCAGCG CTTCCGAGTG CGCGCGCAGG ATGAAGAAGT GCATCAGGTG 
CCAGGTGGTC GTCAGCAAGA AACTGCGCCC AGACGGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164160.1
CDS	376..2637
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRVRAQDEEVHQVPGGRQQETAPRRL

Target ORF information:

RefSeq Version	NM_001170689.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170689.2

ORF Insert Sequence:

ATGGACCCAG ACCCCCAGGC GGGCGTGCAG GTGGGCATGC GGGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTTGGC 
CGCCACGGCA GCCCCTCGAC ACCCGACCGC ACAGTGGTCG TGCAGTGGGA CCAGGGCACG 
CGCACCAACT ACCGCGCCGG CTACCAGGGC GCGCACGACC TGCTGCTGTA CGACAACGCC 
CAGATCGGCG TCCGGCACCC CAACATCATC TGTGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGGATGCGCT GGAAGTGCCG TGTGTGCCTG GACTACGACC TCTGCACGCA GTGCTACATG 
CACAACAAGC ATGAGCTCGC CCACGCCTTC GACCGCTACG AGACCGCTCA CTCGCGCCCT 
GTCACACTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAC TAAGGGGCAT CTTCCAGGGA 
GCGAAGGTGG TGCGAGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGT TTATCGGACA GACGGGCACC GTGCATCGTA TCACGGACCG CGGGGACGTG 
CGCGTGCAGT TCAACCACGA GACGCGCTGG ACCTTCCACC CCGGGGCGCT CACCAAGCAC 
CACTCCTTCT GGGTGGGCGA CGTGGTCCGG GTCATCGGCG ACCTTGACAC AGTGAAGCGG 
CTGCAGGCTG GGCATGGCGA GTGGACGGAC GACATGGCCC CTGCCCTGGG CCGCGTCGGG 
AAGGTGGTGA AAGTGTTTGG AGACGGGAAC CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG 
ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC CGGCCCGAGG AGGATGCCAA CCTGGACGTG 
GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA CTGAGCGTGG CCCTGGACAA GCTTCGGGCC 
CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG CTGGTGGTGG AGGTGGCGCT GGGTAACGCA 
GCCCGGGCTC TGGACCTGCT GCGGAGGCGC CCAGAGCAGG TGGACACCAA GAACCAAGGC 
AGGACCGCTC TGCAAGTGGC TGCCTACCTG GGCCAGGTGG AGTTGATACG GCTGCTGCTA 
CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC GACGAGGGCA ACACGGCACT GCACTACGCG 
GCCCTGGGGA ACCAGCCCGA GGCCACCAGG GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC 
GCCATCAACA GCACCCAGAG CACAGCACTG CACGTGGCCG TGCAGAGGGG CTTCCTGGAG 
GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT GACGTCAACC TGCCCGACGC CCACTCGGAC 
ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC ACTGGAGCCA GCGGCATTGT CGAGGTCCTC 
ACGGAGGTGC CAAACATCGA TGTTACCGCC ACCAACAGCC AGGGTTTCAC CCTGCTGCAC 
CATGCCTCCC TCAAGGGTCA CGCGCTAGCT GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG 
CTGGTGGACG CCAAGAAGGA GGACGGCTTC ACGGCGCTGC ATCTGGCTGC CCTCAACAAC 
CACCGCGAGG TGGCCCAGAT CCTCATCCGG GAGGGCCGCT GTGACGTGAA CGTGCGCAAC 
CGGAAGCTGC AGTCCCCGCT GCATCTCGCC GTGCAACAGG CCCACGTGGG GCTGGTGCCG 
CTACTGGTGG ACGCTGGGTG CAGTGTCAAC GCCGAGGACG AGGAGGGGGA CACAGCCCTG 
CACGTGGCGC TGCAGCGTCA TCAGCTGCTG CCCCTGGTGG CTGATGGGGC CGGGGGGGAC 
CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG 
CTGACGGTGG GCGCGGCGGT CGCCTGCTTC CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC 
ACCAACCACC GCGGTCGGAG CCCGCTGGAC CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC 
CTTCAGGGCT GCGCCCAGCG CTTCCGAGTG CGCGCGCAGG ATGAAGAAGT GCATCAGGTG 
CCAGGTGGTC GTCAGCAAGA AACTGCGCCC AGACGGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( NM_001170689.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001164160.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 5, mRNA.

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CloneID	OHu12048
Clone ID Related Accession (Same CDS sequence)	NM_001170689.2 , NM_001170689.1
Accession Version	NM_001170689.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2262bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA391220.1, DA767833.1, AK128167.1, AL691432.54 and BC037542.1. Transcript Variant: This variant (5) differs in the 5' UTR, lacks a portion of the 5' coding region, uses a downstream start codon, lacks an alternate in-frame exon in the central coding region, and lacks an alternate exon that results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (5) has a shorter N-terminus, a distinct C-terminus, and is overall shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AK128167.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2157511 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCAG ACCCCCAGGC GGGCGTGCAG GTGGGCATGC GGGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTTGGC 
CGCCACGGCA GCCCCTCGAC ACCCGACCGC ACAGTGGTCG TGCAGTGGGA CCAGGGCACG 
CGCACCAACT ACCGCGCCGG CTACCAGGGC GCGCACGACC TGCTGCTGTA CGACAACGCC 
CAGATCGGCG TCCGGCACCC CAACATCATC TGTGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGGATGCGCT GGAAGTGCCG TGTGTGCCTG GACTACGACC TCTGCACGCA GTGCTACATG 
CACAACAAGC ATGAGCTCGC CCACGCCTTC GACCGCTACG AGACCGCTCA CTCGCGCCCT 
GTCACACTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAC TAAGGGGCAT CTTCCAGGGA 
GCGAAGGTGG TGCGAGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGT TTATCGGACA GACGGGCACC GTGCATCGTA TCACGGACCG CGGGGACGTG 
CGCGTGCAGT TCAACCACGA GACGCGCTGG ACCTTCCACC CCGGGGCGCT CACCAAGCAC 
CACTCCTTCT GGGTGGGCGA CGTGGTCCGG GTCATCGGCG ACCTTGACAC AGTGAAGCGG 
CTGCAGGCTG GGCATGGCGA GTGGACGGAC GACATGGCCC CTGCCCTGGG CCGCGTCGGG 
AAGGTGGTGA AAGTGTTTGG AGACGGGAAC CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG 
ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC CGGCCCGAGG AGGATGCCAA CCTGGACGTG 
GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA CTGAGCGTGG CCCTGGACAA GCTTCGGGCC 
CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG CTGGTGGTGG AGGTGGCGCT GGGTAACGCA 
GCCCGGGCTC TGGACCTGCT GCGGAGGCGC CCAGAGCAGG TGGACACCAA GAACCAAGGC 
AGGACCGCTC TGCAAGTGGC TGCCTACCTG GGCCAGGTGG AGTTGATACG GCTGCTGCTA 
CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC GACGAGGGCA ACACGGCACT GCACTACGCG 
GCCCTGGGGA ACCAGCCCGA GGCCACCAGG GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC 
GCCATCAACA GCACCCAGAG CACAGCACTG CACGTGGCCG TGCAGAGGGG CTTCCTGGAG 
GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT GACGTCAACC TGCCCGACGC CCACTCGGAC 
ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC ACTGGAGCCA GCGGCATTGT CGAGGTCCTC 
ACGGAGGTGC CAAACATCGA TGTTACCGCC ACCAACAGCC AGGGTTTCAC CCTGCTGCAC 
CATGCCTCCC TCAAGGGTCA CGCGCTAGCT GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG 
CTGGTGGACG CCAAGAAGGA GGACGGCTTC ACGGCGCTGC ATCTGGCTGC CCTCAACAAC 
CACCGCGAGG TGGCCCAGAT CCTCATCCGG GAGGGCCGCT GTGACGTGAA CGTGCGCAAC 
CGGAAGCTGC AGTCCCCGCT GCATCTCGCC GTGCAACAGG CCCACGTGGG GCTGGTGCCG 
CTACTGGTGG ACGCTGGGTG CAGTGTCAAC GCCGAGGACG AGGAGGGGGA CACAGCCCTG 
CACGTGGCGC TGCAGCGTCA TCAGCTGCTG CCCCTGGTGG CTGATGGGGC CGGGGGGGAC 
CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG 
CTGACGGTGG GCGCGGCGGT CGCCTGCTTC CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC 
ACCAACCACC GCGGTCGGAG CCCGCTGGAC CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC 
CTTCAGGGCT GCGCCCAGCG CTTCCGAGTG CGCGCGCAGG ATGAAGAAGT GCATCAGGTG 
CCAGGTGGTC GTCAGCAAGA AACTGCGCCC AGACGGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164160.1
CDS	366..2627
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRVRAQDEEVHQVPGGRQQETAPRRL

Target ORF information:

RefSeq Version	NM_001170689.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170689.1

ORF Insert Sequence:

ATGGACCCAG ACCCCCAGGC GGGCGTGCAG GTGGGCATGC GGGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTTGGC 
CGCCACGGCA GCCCCTCGAC ACCCGACCGC ACAGTGGTCG TGCAGTGGGA CCAGGGCACG 
CGCACCAACT ACCGCGCCGG CTACCAGGGC GCGCACGACC TGCTGCTGTA CGACAACGCC 
CAGATCGGCG TCCGGCACCC CAACATCATC TGTGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGGATGCGCT GGAAGTGCCG TGTGTGCCTG GACTACGACC TCTGCACGCA GTGCTACATG 
CACAACAAGC ATGAGCTCGC CCACGCCTTC GACCGCTACG AGACCGCTCA CTCGCGCCCT 
GTCACACTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAC TAAGGGGCAT CTTCCAGGGA 
GCGAAGGTGG TGCGAGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGT TTATCGGACA GACGGGCACC GTGCATCGTA TCACGGACCG CGGGGACGTG 
CGCGTGCAGT TCAACCACGA GACGCGCTGG ACCTTCCACC CCGGGGCGCT CACCAAGCAC 
CACTCCTTCT GGGTGGGCGA CGTGGTCCGG GTCATCGGCG ACCTTGACAC AGTGAAGCGG 
CTGCAGGCTG GGCATGGCGA GTGGACGGAC GACATGGCCC CTGCCCTGGG CCGCGTCGGG 
AAGGTGGTGA AAGTGTTTGG AGACGGGAAC CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG 
ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC CGGCCCGAGG AGGATGCCAA CCTGGACGTG 
GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA CTGAGCGTGG CCCTGGACAA GCTTCGGGCC 
CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG CTGGTGGTGG AGGTGGCGCT GGGTAACGCA 
GCCCGGGCTC TGGACCTGCT GCGGAGGCGC CCAGAGCAGG TGGACACCAA GAACCAAGGC 
AGGACCGCTC TGCAAGTGGC TGCCTACCTG GGCCAGGTGG AGTTGATACG GCTGCTGCTA 
CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC GACGAGGGCA ACACGGCACT GCACTACGCG 
GCCCTGGGGA ACCAGCCCGA GGCCACCAGG GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC 
GCCATCAACA GCACCCAGAG CACAGCACTG CACGTGGCCG TGCAGAGGGG CTTCCTGGAG 
GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT GACGTCAACC TGCCCGACGC CCACTCGGAC 
ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC ACTGGAGCCA GCGGCATTGT CGAGGTCCTC 
ACGGAGGTGC CAAACATCGA TGTTACCGCC ACCAACAGCC AGGGTTTCAC CCTGCTGCAC 
CATGCCTCCC TCAAGGGTCA CGCGCTAGCT GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG 
CTGGTGGACG CCAAGAAGGA GGACGGCTTC ACGGCGCTGC ATCTGGCTGC CCTCAACAAC 
CACCGCGAGG TGGCCCAGAT CCTCATCCGG GAGGGCCGCT GTGACGTGAA CGTGCGCAAC 
CGGAAGCTGC AGTCCCCGCT GCATCTCGCC GTGCAACAGG CCCACGTGGG GCTGGTGCCG 
CTACTGGTGG ACGCTGGGTG CAGTGTCAAC GCCGAGGACG AGGAGGGGGA CACAGCCCTG 
CACGTGGCGC TGCAGCGTCA TCAGCTGCTG CCCCTGGTGG CTGATGGGGC CGGGGGGGAC 
CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG 
CTGACGGTGG GCGCGGCGGT CGCCTGCTTC CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC 
ACCAACCACC GCGGTCGGAG CCCGCTGGAC CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC 
CTTCAGGGCT GCGCCCAGCG CTTCCGAGTG CGCGCGCAGG ATGAAGAAGT GCATCAGGTG 
CCAGGTGGTC GTCAGCAAGA AACTGCGCCC AGACGGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( NM_001170687.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001164158.2 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 3, mRNA.

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CloneID	OHu107540
Clone ID Related Accession (Same CDS sequence)	NM_001170687.2
Accession Version	NM_001170687.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL691432.54 and FO704657.2. On Dec 21, 2018 this sequence version replaced NM_001170687.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB076691.1, BC037542.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTTGGA AGCCCAGCGA GGCTAGAGGC CAGTCCCAAA GTTTCCAGGC ATCAGGGCTG 
CAGCCCAGGA GCCTCAAGGC GGCCCGGCGG GCGACTGGAC GGCCGGACAG GTCCCGAGCA 
GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC AGGTGGGCAT GCGGGTGGTG 
CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG AGGGCGGCGT GGGCACGGTG 
GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC GCACAGTGGT CGTGCAGTGG 
GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG GCGCGCACGA CCTGCTGCTG 
TACGACAACG CCCAGATCGG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGCACCAC TCCTTCTGGG TGGGCGACGT GGTCCGGGTC 
ATCGGCGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACGGACGAC 
ATGGCCCCTG CCCTGGGCCG CGTCGGGAAG GTGGTGAAAG TGTTTGGAGA CGGGAACCTG 
CGTGTAGCAG TCGCTGGTCA GCGGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCCGAGGAGG ATGCCAACCT GGACGTGGCC GAGCGCGCCC GGGAGAACAA AAGCTCACTG 
AGCGTGGCCC TGGACAAGCT TCGGGCCCAG AAGAGTGACC CAGAGCACCC GGGAAGGCTG 
GTGGTGGAGG TGGCGCTGGG TAACGCAGCC CGGGCTCTGG ACCTGCTGCG GAGGCGCCCA 
GAGCAGGTGG ACACCAAGAA CCAAGGCAGG ACCGCTCTGC AAGTGGCTGC CTACCTGGGC 
CAGGTGGAGT TGATACGGCT GCTGCTACAA GCCAGGGCGG GCGTGGACCT GCCGGACGAC 
GAGGGCAACA CGGCACTGCA CTACGCGGCC CTGGGGAACC AGCCCGAGGC CACCAGGGTG 
CTCCTGAGTG CTGGGTGCCG GGCGGACGCC ATCAACAGCA CCCAGAGCAC AGCACTGCAC 
GTGGCCGTGC AGAGGGGCTT CCTGGAGGTG GTGCGGGCCC TGTGTGAGCG CGGCTGTGAC 
GTCAACCTGC CCGACGCCCA CTCGGACACG CCCCTGCACT CCGCCATCTC GGCGGGCACT 
GGAGCCAGCG GCATTGTCGA GGTCCTCACG GAGGTGCCAA ACATCGATGT TACCGCCACC 
AACAGCCAGG GTTTCACCCT GCTGCACCAT GCCTCCCTCA AGGGTCACGC GCTAGCTGTG 
AGAAAGATTC TGGCTCGGGC GCGGCAGCTG GTGGACGCCA AGAAGGAGGA CGGCTTCACG 
GCGCTGCATC TGGCTGCCCT CAACAACCAC CGCGAGGTGG CCCAGATCCT CATCCGGGAG 
GGCCGCTGTG ACGTGAACGT GCGCAACCGG AAGCTGCAGT CCCCGCTGCA TCTCGCCGTG 
CAACAGGCCC ACGTGGGGCT GGTGCCGCTA CTGGTGGACG CTGGGTGCAG TGTCAACGCC 
GAGGACGAGG AGGGGGACAC AGCCCTGCAC GTGGCGCTGC AGCGTCATCA GCTGCTGCCC 
CTGGTGGCTG ATGGGGCCGG GGGGGACCCA GGGCCCTTGC AGCTGCTGTC CAGGCTACAG 
GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG 
GCGCTGGAGG GCGCCGACGT GAGCTACACC AACCACCGCG GTCGGAGCCC GCTGGACCTG 
GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG 
CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC AGGCAAACGC TCGGGACCCC CAACACCGTG 
ACGAACCTGC ACGTGGGCGC CGCGCCGGGG CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG TGCCAGCACC GCACCGTGTG TGAGGAGTGC 
GCGCGCAGGA TGAAGAAGTG CATCAGGTGC CAGGTGGTCG TCAGCAAGAA ACTGCGCCCA 
GACGGCTCTG AGGTGGCGAG CGCCGCCCCC GCCCCCGGCC CGCCGCGCCA GCTGGTGGAG 
GAGCTGCAGA GCCGCTACCG GCAGATGGAG GAACGCATCA CCTGCCCCAT CTGCATCGAC 
AGCCACATCC GCCTCGTGTT CCAGTGCGGC CACGGCGCAT GCGCCCCCTG CGGCTCCGCG 
CTCAGCGCCT GCCCCATCTG CCGCCAGCCC ATCCGCGACC GCATCCAGAT CTTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164158.2
CDS	132..3131
Translation	MGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_001170687.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170687.2

ORF Insert Sequence:

ATGGGTTGGA AGCCCAGCGA GGCTAGAGGC CAGTCCCAAA GTTTCCAGGC ATCAGGGCTG 
CAGCCCAGGA GCCTCAAGGC GGCCCGGCGG GCGACTGGAC GGCCGGACAG GTCCCGAGCA 
GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC AGGTGGGCAT GCGGGTGGTG 
CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG AGGGCGGCGT GGGCACGGTG 
GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC GCACAGTGGT CGTGCAGTGG 
GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG GCGCGCACGA CCTGCTGCTG 
TACGACAACG CCCAGATCGG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGCACCAC TCCTTCTGGG TGGGCGACGT GGTCCGGGTC 
ATCGGCGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACGGACGAC 
ATGGCCCCTG CCCTGGGCCG CGTCGGGAAG GTGGTGAAAG TGTTTGGAGA CGGGAACCTG 
CGTGTAGCAG TCGCTGGTCA GCGGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCCGAGGAGG ATGCCAACCT GGACGTGGCC GAGCGCGCCC GGGAGAACAA AAGCTCACTG 
AGCGTGGCCC TGGACAAGCT TCGGGCCCAG AAGAGTGACC CAGAGCACCC GGGAAGGCTG 
GTGGTGGAGG TGGCGCTGGG TAACGCAGCC CGGGCTCTGG ACCTGCTGCG GAGGCGCCCA 
GAGCAGGTGG ACACCAAGAA CCAAGGCAGG ACCGCTCTGC AAGTGGCTGC CTACCTGGGC 
CAGGTGGAGT TGATACGGCT GCTGCTACAA GCCAGGGCGG GCGTGGACCT GCCGGACGAC 
GAGGGCAACA CGGCACTGCA CTACGCGGCC CTGGGGAACC AGCCCGAGGC CACCAGGGTG 
CTCCTGAGTG CTGGGTGCCG GGCGGACGCC ATCAACAGCA CCCAGAGCAC AGCACTGCAC 
GTGGCCGTGC AGAGGGGCTT CCTGGAGGTG GTGCGGGCCC TGTGTGAGCG CGGCTGTGAC 
GTCAACCTGC CCGACGCCCA CTCGGACACG CCCCTGCACT CCGCCATCTC GGCGGGCACT 
GGAGCCAGCG GCATTGTCGA GGTCCTCACG GAGGTGCCAA ACATCGATGT TACCGCCACC 
AACAGCCAGG GTTTCACCCT GCTGCACCAT GCCTCCCTCA AGGGTCACGC GCTAGCTGTG 
AGAAAGATTC TGGCTCGGGC GCGGCAGCTG GTGGACGCCA AGAAGGAGGA CGGCTTCACG 
GCGCTGCATC TGGCTGCCCT CAACAACCAC CGCGAGGTGG CCCAGATCCT CATCCGGGAG 
GGCCGCTGTG ACGTGAACGT GCGCAACCGG AAGCTGCAGT CCCCGCTGCA TCTCGCCGTG 
CAACAGGCCC ACGTGGGGCT GGTGCCGCTA CTGGTGGACG CTGGGTGCAG TGTCAACGCC 
GAGGACGAGG AGGGGGACAC AGCCCTGCAC GTGGCGCTGC AGCGTCATCA GCTGCTGCCC 
CTGGTGGCTG ATGGGGCCGG GGGGGACCCA GGGCCCTTGC AGCTGCTGTC CAGGCTACAG 
GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG 
GCGCTGGAGG GCGCCGACGT GAGCTACACC AACCACCGCG GTCGGAGCCC GCTGGACCTG 
GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG 
CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC AGGCAAACGC TCGGGACCCC CAACACCGTG 
ACGAACCTGC ACGTGGGCGC CGCGCCGGGG CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG TGCCAGCACC GCACCGTGTG TGAGGAGTGC 
GCGCGCAGGA TGAAGAAGTG CATCAGGTGC CAGGTGGTCG TCAGCAAGAA ACTGCGCCCA 
GACGGCTCTG AGGTGGCGAG CGCCGCCCCC GCCCCCGGCC CGCCGCGCCA GCTGGTGGAG 
GAGCTGCAGA GCCGCTACCG GCAGATGGAG GAACGCATCA CCTGCCCCAT CTGCATCGAC 
AGCCACATCC GCCTCGTGTT CCAGTGCGGC CACGGCGCAT GCGCCCCCTG CGGCTCCGCG 
CTCAGCGCCT GCCCCATCTG CCGCCAGCCC ATCCGCGACC GCATCCAGAT CTTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( NM_001170686.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001164157.2 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu107534
Clone ID Related Accession (Same CDS sequence)	NM_001170686.2
Accession Version	NM_001170686.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL691432.54 and FO704657.2. On Dec 21, 2018 this sequence version replaced NM_001170686.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB076693.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGTTGGA AGCCCAGCGA GGCTAGAGGC CAGTCCCAAA GTTTCCAGGC ATCAGGGCTG 
CAGCCCAGGA GCCTCAAGGC GGCCCGGCGG GCGACTGGAC GGCCGGACAG GTCCCGAGCA 
GCCCGGCCCA CCATGGACCC CTCTGCCCAC AGGTCCCGAG CAGCCCCGCC CAACATGGAC 
CCAGACCCCC AGGCGGGCGT GCAGGTGGGC ATGCGGGTGG TGCGCGGCGT GGACTGGAAG 
TGGGGCCAGC AGGACGGCGG CGAGGGCGGC GTGGGCACGG TGGTGGAGCT TGGCCGCCAC 
GGCAGCCCCT CGACACCCGA CCGCACAGTG GTCGTGCAGT GGGACCAGGG CACGCGCACC 
AACTACCGCG CCGGCTACCA GGGCGCGCAC GACCTGCTGC TGTACGACAA CGCCCAGATC 
GGCGTCCGGC ACCCCAACAT CATCTGTGAC TGCTGCAAGA AGCACGGGCT GCGGGGGATG 
CGCTGGAAGT GCCGTGTGTG CCTGGACTAC GACCTCTGCA CGCAGTGCTA CATGCACAAC 
AAGCATGAGC TCGCCCACGC CTTCGACCGC TACGAGACCG CTCACTCGCG CCCTGTCACA 
CTGAGTCCCC GCCAGGGCCT CCCGAGGATC CCACTAAGGG GCATCTTCCA GGGAGCGAAG 
GTGGTGCGAG GCCCCGACTG GGAGTGGGGC TCACAGGATG GAGGGGAAGG GAAACCGGGC 
CGTGTGGTGG ACATCCGTGG CTGGGATGTG GAGACAGGCC GGAGTGTGGC CAGCGTGACG 
TGGGCTGATG GTACCACCAA TGTGTACCGT GTGGGCCACA AGGGCAAGGT GGACCTCAAG 
TGTGTGGGCG AGGCAGCGGG CGGCTTCTAC TACAAGGACC ACCTCCCAAG GCTCGGCAAG 
CCGGCGGAGC TGCAGCGCAG GGTGAGTGCT GACAGCCAGC CCTTCCAGCA CGGGGACAAG 
GTCAAGTGTC TGCTGGACAC TGATGTCCTG CGGGAGATGC AGGAAGGCCA CGGCGGCTGG 
AACCCCAGGA TGGCGGAGAC GGGCACCGTG CATCGTATCA CGGACCGCGG GGACGTGCGC 
GTGCAGTTCA ACCACGAGAC GCGCTGGACC TTCCACCCCG GGGCGCTCAC CAAGCACCAC 
TCCTTCTGGG TGGGCGACGT GGTCCGGGTC ATCGGCGACC TTGACACAGT GAAGCGGCTG 
CAGGCTGGGC ATGGCGAGTG GACGGACGAC ATGGCCCCTG CCCTGGGCCG CGTCGGGAAG 
GTGGTGAAAG TGTTTGGAGA CGGGAACCTG CGTGTAGCAG TCGCTGGTCA GCGGTGGACC 
TTCAGCCCCT CCTGCCTGGT GGCCTACCGG CCCGAGGAGG ATGCCAACCT GGACGTGGCC 
GAGCGCGCCC GGGAGAACAA AAGCTCACTG AGCGTGGCCC TGGACAAGCT TCGGGCCCAG 
AAGAGTGACC CAGAGCACCC GGGAAGGCTG GTGGTGGAGG TGGCGCTGGG TAACGCAGCC 
CGGGCTCTGG ACCTGCTGCG GAGGCGCCCA GAGCAGGTGG ACACCAAGAA CCAAGGCAGG 
ACCGCTCTGC AAGTGGCTGC CTACCTGGGC CAGGTGGAGT TGATACGGCT GCTGCTACAA 
GCCAGGGCGG GCGTGGACCT GCCGGACGAC GAGGGCAACA CGGCACTGCA CTACGCGGCC 
CTGGGGAACC AGCCCGAGGC CACCAGGGTG CTCCTGAGTG CTGGGTGCCG GGCGGACGCC 
ATCAACAGCA CCCAGAGCAC AGCACTGCAC GTGGCCGTGC AGAGGGGCTT CCTGGAGGTG 
GTGCGGGCCC TGTGTGAGCG CGGCTGTGAC GTCAACCTGC CCGACGCCCA CTCGGACACG 
CCCCTGCACT CCGCCATCTC GGCGGGCACT GGAGCCAGCG GCATTGTCGA GGTCCTCACG 
GAGGTGCCAA ACATCGATGT TACCGCCACC AACAGCCAGG GTTTCACCCT GCTGCACCAT 
GCCTCCCTCA AGGGTCACGC GCTAGCTGTG AGAAAGATTC TGGCTCGGGC GCGGCAGCTG 
GTGGACGCCA AGAAGGAGGA CGGCTTCACG GCGCTGCATC TGGCTGCCCT CAACAACCAC 
CGCGAGGTGG CCCAGATCCT CATCCGGGAG GGCCGCTGTG ACGTGAACGT GCGCAACCGG 
AAGCTGCAGT CCCCGCTGCA TCTCGCCGTG CAACAGGCCC ACGTGGGGCT GGTGCCGCTA 
CTGGTGGACG CTGGGTGCAG TGTCAACGCC GAGGACGAGG AGGGGGACAC AGCCCTGCAC 
GTGGCGCTGC AGCGTCATCA GCTGCTGCCC CTGGTGGCTG ATGGGGCCGG GGGGGACCCA 
GGGCCCTTGC AGCTGCTGTC CAGGCTACAG GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG 
ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG GCGCTGGAGG GCGCCGACGT GAGCTACACC 
AACCACCGCG GTCGGAGCCC GCTGGACCTG GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT 
CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC 
AGGCAAACGC TCGGGACCCC CAACACCGTG ACGAACCTGC ACGTGGGCGC CGCGCCGGGG 
CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG 
TGCCAGCACC GCACCGTGTG TGAGGAGTGC GCGCGCAGGA TGAAGAAGTG CATCAGGTGC 
CAGGTGGTCG TCAGCAAGAA ACTGCGCCCA GACGGCTCTG AGGTGGCGAG CGCCGCCCCC 
GCCCCCGGCC CGCCGCGCCA GCTGGTGGAG GAGCTGCAGA GCCGCTACCG GCAGATGGAG 
GAACGCATCA CCTGCCCCAT CTGCATCGAC AGCCACATCC GCCTCGTGTT CCAGTGCGGC 
CACGGCGCAT GCGCCCCCTG CGGCTCCGCG CTCAGCGCCT GCCCCATCTG CCGCCAGCCC 
ATCCGCGACC GCATCCAGAT CTTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164157.2
CDS	132..3161
Translation	MGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAARPTMDPSAHRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAETGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_001170686.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170686.2

ORF Insert Sequence:

ATGGGTTGGA AGCCCAGCGA GGCTAGAGGC CAGTCCCAAA GTTTCCAGGC ATCAGGGCTG 
CAGCCCAGGA GCCTCAAGGC GGCCCGGCGG GCGACTGGAC GGCCGGACAG GTCCCGAGCA 
GCCCGGCCCA CCATGGACCC CTCTGCCCAC AGGTCCCGAG CAGCCCCGCC CAACATGGAC 
CCAGACCCCC AGGCGGGCGT GCAGGTGGGC ATGCGGGTGG TGCGCGGCGT GGACTGGAAG 
TGGGGCCAGC AGGACGGCGG CGAGGGCGGC GTGGGCACGG TGGTGGAGCT TGGCCGCCAC 
GGCAGCCCCT CGACACCCGA CCGCACAGTG GTCGTGCAGT GGGACCAGGG CACGCGCACC 
AACTACCGCG CCGGCTACCA GGGCGCGCAC GACCTGCTGC TGTACGACAA CGCCCAGATC 
GGCGTCCGGC ACCCCAACAT CATCTGTGAC TGCTGCAAGA AGCACGGGCT GCGGGGGATG 
CGCTGGAAGT GCCGTGTGTG CCTGGACTAC GACCTCTGCA CGCAGTGCTA CATGCACAAC 
AAGCATGAGC TCGCCCACGC CTTCGACCGC TACGAGACCG CTCACTCGCG CCCTGTCACA 
CTGAGTCCCC GCCAGGGCCT CCCGAGGATC CCACTAAGGG GCATCTTCCA GGGAGCGAAG 
GTGGTGCGAG GCCCCGACTG GGAGTGGGGC TCACAGGATG GAGGGGAAGG GAAACCGGGC 
CGTGTGGTGG ACATCCGTGG CTGGGATGTG GAGACAGGCC GGAGTGTGGC CAGCGTGACG 
TGGGCTGATG GTACCACCAA TGTGTACCGT GTGGGCCACA AGGGCAAGGT GGACCTCAAG 
TGTGTGGGCG AGGCAGCGGG CGGCTTCTAC TACAAGGACC ACCTCCCAAG GCTCGGCAAG 
CCGGCGGAGC TGCAGCGCAG GGTGAGTGCT GACAGCCAGC CCTTCCAGCA CGGGGACAAG 
GTCAAGTGTC TGCTGGACAC TGATGTCCTG CGGGAGATGC AGGAAGGCCA CGGCGGCTGG 
AACCCCAGGA TGGCGGAGAC GGGCACCGTG CATCGTATCA CGGACCGCGG GGACGTGCGC 
GTGCAGTTCA ACCACGAGAC GCGCTGGACC TTCCACCCCG GGGCGCTCAC CAAGCACCAC 
TCCTTCTGGG TGGGCGACGT GGTCCGGGTC ATCGGCGACC TTGACACAGT GAAGCGGCTG 
CAGGCTGGGC ATGGCGAGTG GACGGACGAC ATGGCCCCTG CCCTGGGCCG CGTCGGGAAG 
GTGGTGAAAG TGTTTGGAGA CGGGAACCTG CGTGTAGCAG TCGCTGGTCA GCGGTGGACC 
TTCAGCCCCT CCTGCCTGGT GGCCTACCGG CCCGAGGAGG ATGCCAACCT GGACGTGGCC 
GAGCGCGCCC GGGAGAACAA AAGCTCACTG AGCGTGGCCC TGGACAAGCT TCGGGCCCAG 
AAGAGTGACC CAGAGCACCC GGGAAGGCTG GTGGTGGAGG TGGCGCTGGG TAACGCAGCC 
CGGGCTCTGG ACCTGCTGCG GAGGCGCCCA GAGCAGGTGG ACACCAAGAA CCAAGGCAGG 
ACCGCTCTGC AAGTGGCTGC CTACCTGGGC CAGGTGGAGT TGATACGGCT GCTGCTACAA 
GCCAGGGCGG GCGTGGACCT GCCGGACGAC GAGGGCAACA CGGCACTGCA CTACGCGGCC 
CTGGGGAACC AGCCCGAGGC CACCAGGGTG CTCCTGAGTG CTGGGTGCCG GGCGGACGCC 
ATCAACAGCA CCCAGAGCAC AGCACTGCAC GTGGCCGTGC AGAGGGGCTT CCTGGAGGTG 
GTGCGGGCCC TGTGTGAGCG CGGCTGTGAC GTCAACCTGC CCGACGCCCA CTCGGACACG 
CCCCTGCACT CCGCCATCTC GGCGGGCACT GGAGCCAGCG GCATTGTCGA GGTCCTCACG 
GAGGTGCCAA ACATCGATGT TACCGCCACC AACAGCCAGG GTTTCACCCT GCTGCACCAT 
GCCTCCCTCA AGGGTCACGC GCTAGCTGTG AGAAAGATTC TGGCTCGGGC GCGGCAGCTG 
GTGGACGCCA AGAAGGAGGA CGGCTTCACG GCGCTGCATC TGGCTGCCCT CAACAACCAC 
CGCGAGGTGG CCCAGATCCT CATCCGGGAG GGCCGCTGTG ACGTGAACGT GCGCAACCGG 
AAGCTGCAGT CCCCGCTGCA TCTCGCCGTG CAACAGGCCC ACGTGGGGCT GGTGCCGCTA 
CTGGTGGACG CTGGGTGCAG TGTCAACGCC GAGGACGAGG AGGGGGACAC AGCCCTGCAC 
GTGGCGCTGC AGCGTCATCA GCTGCTGCCC CTGGTGGCTG ATGGGGCCGG GGGGGACCCA 
GGGCCCTTGC AGCTGCTGTC CAGGCTACAG GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG 
ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG GCGCTGGAGG GCGCCGACGT GAGCTACACC 
AACCACCGCG GTCGGAGCCC GCTGGACCTG GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT 
CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC 
AGGCAAACGC TCGGGACCCC CAACACCGTG ACGAACCTGC ACGTGGGCGC CGCGCCGGGG 
CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG 
TGCCAGCACC GCACCGTGTG TGAGGAGTGC GCGCGCAGGA TGAAGAAGTG CATCAGGTGC 
CAGGTGGTCG TCAGCAAGAA ACTGCGCCCA GACGGCTCTG AGGTGGCGAG CGCCGCCCCC 
GCCCCCGGCC CGCCGCGCCA GCTGGTGGAG GAGCTGCAGA GCCGCTACCG GCAGATGGAG 
GAACGCATCA CCTGCCCCAT CTGCATCGAC AGCCACATCC GCCTCGTGTT CCAGTGCGGC 
CACGGCGCAT GCGCCCCCTG CGGCTCCGCG CTCAGCGCCT GCCCCATCTG CCGCCAGCCC 
ATCCGCGACC GCATCCAGAT CTTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( NM_080875.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_543151.3 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu107536
Clone ID Related Accession (Same CDS sequence)	NM_080875.3
Accession Version	NM_080875.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3042bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL691432.54 and FO704657.2. This sequence is a reference standard in the RefSeqGene project. On Dec 21, 2018 this sequence version replaced NM_080875.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB074480.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTTGGA AGCCCAGCGA GGCTAGAGGC CAGTCCCAAA GTTTCCAGGC ATCAGGGCTG 
CAGCCCAGGA GCCTCAAGGC GGCCCGGCGG GCGACTGGAC GGCCGGACAG GTCCCGAGCA 
GCCCGGCCCA CCATGGACCC CTCTGCCCAC AGGTCCCGAG CAGCCCCGCC CAACATGGAC 
CCAGACCCCC AGGCGGGCGT GCAGGTGGGC ATGCGGGTGG TGCGCGGCGT GGACTGGAAG 
TGGGGCCAGC AGGACGGCGG CGAGGGCGGC GTGGGCACGG TGGTGGAGCT TGGCCGCCAC 
GGCAGCCCCT CGACACCCGA CCGCACAGTG GTCGTGCAGT GGGACCAGGG CACGCGCACC 
AACTACCGCG CCGGCTACCA GGGCGCGCAC GACCTGCTGC TGTACGACAA CGCCCAGATC 
GGCGTCCGGC ACCCCAACAT CATCTGTGAC TGCTGCAAGA AGCACGGGCT GCGGGGGATG 
CGCTGGAAGT GCCGTGTGTG CCTGGACTAC GACCTCTGCA CGCAGTGCTA CATGCACAAC 
AAGCATGAGC TCGCCCACGC CTTCGACCGC TACGAGACCG CTCACTCGCG CCCTGTCACA 
CTGAGTCCCC GCCAGGGCCT CCCGAGGATC CCACTAAGGG GCATCTTCCA GGGAGCGAAG 
GTGGTGCGAG GCCCCGACTG GGAGTGGGGC TCACAGGATG GAGGGGAAGG GAAACCGGGC 
CGTGTGGTGG ACATCCGTGG CTGGGATGTG GAGACAGGCC GGAGTGTGGC CAGCGTGACG 
TGGGCTGATG GTACCACCAA TGTGTACCGT GTGGGCCACA AGGGCAAGGT GGACCTCAAG 
TGTGTGGGCG AGGCAGCGGG CGGCTTCTAC TACAAGGACC ACCTCCCAAG GCTCGGCAAG 
CCGGCGGAGC TGCAGCGCAG GGTGAGTGCT GACAGCCAGC CCTTCCAGCA CGGGGACAAG 
GTCAAGTGTC TGCTGGACAC TGATGTCCTG CGGGAGATGC AGGAAGGCCA CGGCGGCTGG 
AACCCCAGGA TGGCGGAGTT TATCGGACAG ACGGGCACCG TGCATCGTAT CACGGACCGC 
GGGGACGTGC GCGTGCAGTT CAACCACGAG ACGCGCTGGA CCTTCCACCC CGGGGCGCTC 
ACCAAGCACC ACTCCTTCTG GGTGGGCGAC GTGGTCCGGG TCATCGGCGA CCTTGACACA 
GTGAAGCGGC TGCAGGCTGG GCATGGCGAG TGGACGGACG ACATGGCCCC TGCCCTGGGC 
CGCGTCGGGA AGGTGGTGAA AGTGTTTGGA GACGGGAACC TGCGTGTAGC AGTCGCTGGT 
CAGCGGTGGA CCTTCAGCCC CTCCTGCCTG GTGGCCTACC GGCCCGAGGA GGATGCCAAC 
CTGGACGTGG CCGAGCGCGC CCGGGAGAAC AAAAGCTCAC TGAGCGTGGC CCTGGACAAG 
CTTCGGGCCC AGAAGAGTGA CCCAGAGCAC CCGGGAAGGC TGGTGGTGGA GGTGGCGCTG 
GGTAACGCAG CCCGGGCTCT GGACCTGCTG CGGAGGCGCC CAGAGCAGGT GGACACCAAG 
AACCAAGGCA GGACCGCTCT GCAAGTGGCT GCCTACCTGG GCCAGGTGGA GTTGATACGG 
CTGCTGCTAC AAGCCAGGGC GGGCGTGGAC CTGCCGGACG ACGAGGGCAA CACGGCACTG 
CACTACGCGG CCCTGGGGAA CCAGCCCGAG GCCACCAGGG TGCTCCTGAG TGCTGGGTGC 
CGGGCGGACG CCATCAACAG CACCCAGAGC ACAGCACTGC ACGTGGCCGT GCAGAGGGGC 
TTCCTGGAGG TGGTGCGGGC CCTGTGTGAG CGCGGCTGTG ACGTCAACCT GCCCGACGCC 
CACTCGGACA CGCCCCTGCA CTCCGCCATC TCGGCGGGCA CTGGAGCCAG CGGCATTGTC 
GAGGTCCTCA CGGAGGTGCC AAACATCGAT GTTACCGCCA CCAACAGCCA GGGTTTCACC 
CTGCTGCACC ATGCCTCCCT CAAGGGTCAC GCGCTAGCTG TGAGAAAGAT TCTGGCTCGG 
GCGCGGCAGC TGGTGGACGC CAAGAAGGAG GACGGCTTCA CGGCGCTGCA TCTGGCTGCC 
CTCAACAACC ACCGCGAGGT GGCCCAGATC CTCATCCGGG AGGGCCGCTG TGACGTGAAC 
GTGCGCAACC GGAAGCTGCA GTCCCCGCTG CATCTCGCCG TGCAACAGGC CCACGTGGGG 
CTGGTGCCGC TACTGGTGGA CGCTGGGTGC AGTGTCAACG CCGAGGACGA GGAGGGGGAC 
ACAGCCCTGC ACGTGGCGCT GCAGCGTCAT CAGCTGCTGC CCCTGGTGGC TGATGGGGCC 
GGGGGGGACC CAGGGCCCTT GCAGCTGCTG TCCAGGCTAC AGGCCTCGGG CCTCCCCGGC 
AGCGCGGAGC TGACGGTGGG CGCGGCGGTC GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC 
GTGAGCTACA CCAACCACCG CGGTCGGAGC CCGCTGGACC TGGCCGCCGA GGGTCGCGTG 
CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG 
GCCCCGGGCC CCAGGCAAAC GCTCGGGACC CCCAACACCG TGACGAACCT GCACGTGGGC 
GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG 
CTGTTCTCGC CGTGCCAGCA CCGCACCGTG TGTGAGGAGT GCGCGCGCAG GATGAAGAAG 
TGCATCAGGT GCCAGGTGGT CGTCAGCAAG AAACTGCGCC CAGACGGCTC TGAGGTGGCG 
AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC 
CGGCAGATGG AGGAACGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG 
TTCCAGTGCG GCCACGGCGC ATGCGCCCCC TGCGGCTCCG CGCTCAGCGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_543151.3
CDS	132..3173
Translation	MGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAARPTMDPSAHRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_080875.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080875.3

ORF Insert Sequence:

ATGGGTTGGA AGCCCAGCGA GGCTAGAGGC CAGTCCCAAA GTTTCCAGGC ATCAGGGCTG 
CAGCCCAGGA GCCTCAAGGC GGCCCGGCGG GCGACTGGAC GGCCGGACAG GTCCCGAGCA 
GCCCGGCCCA CCATGGACCC CTCTGCCCAC AGGTCCCGAG CAGCCCCGCC CAACATGGAC 
CCAGACCCCC AGGCGGGCGT GCAGGTGGGC ATGCGGGTGG TGCGCGGCGT GGACTGGAAG 
TGGGGCCAGC AGGACGGCGG CGAGGGCGGC GTGGGCACGG TGGTGGAGCT TGGCCGCCAC 
GGCAGCCCCT CGACACCCGA CCGCACAGTG GTCGTGCAGT GGGACCAGGG CACGCGCACC 
AACTACCGCG CCGGCTACCA GGGCGCGCAC GACCTGCTGC TGTACGACAA CGCCCAGATC 
GGCGTCCGGC ACCCCAACAT CATCTGTGAC TGCTGCAAGA AGCACGGGCT GCGGGGGATG 
CGCTGGAAGT GCCGTGTGTG CCTGGACTAC GACCTCTGCA CGCAGTGCTA CATGCACAAC 
AAGCATGAGC TCGCCCACGC CTTCGACCGC TACGAGACCG CTCACTCGCG CCCTGTCACA 
CTGAGTCCCC GCCAGGGCCT CCCGAGGATC CCACTAAGGG GCATCTTCCA GGGAGCGAAG 
GTGGTGCGAG GCCCCGACTG GGAGTGGGGC TCACAGGATG GAGGGGAAGG GAAACCGGGC 
CGTGTGGTGG ACATCCGTGG CTGGGATGTG GAGACAGGCC GGAGTGTGGC CAGCGTGACG 
TGGGCTGATG GTACCACCAA TGTGTACCGT GTGGGCCACA AGGGCAAGGT GGACCTCAAG 
TGTGTGGGCG AGGCAGCGGG CGGCTTCTAC TACAAGGACC ACCTCCCAAG GCTCGGCAAG 
CCGGCGGAGC TGCAGCGCAG GGTGAGTGCT GACAGCCAGC CCTTCCAGCA CGGGGACAAG 
GTCAAGTGTC TGCTGGACAC TGATGTCCTG CGGGAGATGC AGGAAGGCCA CGGCGGCTGG 
AACCCCAGGA TGGCGGAGTT TATCGGACAG ACGGGCACCG TGCATCGTAT CACGGACCGC 
GGGGACGTGC GCGTGCAGTT CAACCACGAG ACGCGCTGGA CCTTCCACCC CGGGGCGCTC 
ACCAAGCACC ACTCCTTCTG GGTGGGCGAC GTGGTCCGGG TCATCGGCGA CCTTGACACA 
GTGAAGCGGC TGCAGGCTGG GCATGGCGAG TGGACGGACG ACATGGCCCC TGCCCTGGGC 
CGCGTCGGGA AGGTGGTGAA AGTGTTTGGA GACGGGAACC TGCGTGTAGC AGTCGCTGGT 
CAGCGGTGGA CCTTCAGCCC CTCCTGCCTG GTGGCCTACC GGCCCGAGGA GGATGCCAAC 
CTGGACGTGG CCGAGCGCGC CCGGGAGAAC AAAAGCTCAC TGAGCGTGGC CCTGGACAAG 
CTTCGGGCCC AGAAGAGTGA CCCAGAGCAC CCGGGAAGGC TGGTGGTGGA GGTGGCGCTG 
GGTAACGCAG CCCGGGCTCT GGACCTGCTG CGGAGGCGCC CAGAGCAGGT GGACACCAAG 
AACCAAGGCA GGACCGCTCT GCAAGTGGCT GCCTACCTGG GCCAGGTGGA GTTGATACGG 
CTGCTGCTAC AAGCCAGGGC GGGCGTGGAC CTGCCGGACG ACGAGGGCAA CACGGCACTG 
CACTACGCGG CCCTGGGGAA CCAGCCCGAG GCCACCAGGG TGCTCCTGAG TGCTGGGTGC 
CGGGCGGACG CCATCAACAG CACCCAGAGC ACAGCACTGC ACGTGGCCGT GCAGAGGGGC 
TTCCTGGAGG TGGTGCGGGC CCTGTGTGAG CGCGGCTGTG ACGTCAACCT GCCCGACGCC 
CACTCGGACA CGCCCCTGCA CTCCGCCATC TCGGCGGGCA CTGGAGCCAG CGGCATTGTC 
GAGGTCCTCA CGGAGGTGCC AAACATCGAT GTTACCGCCA CCAACAGCCA GGGTTTCACC 
CTGCTGCACC ATGCCTCCCT CAAGGGTCAC GCGCTAGCTG TGAGAAAGAT TCTGGCTCGG 
GCGCGGCAGC TGGTGGACGC CAAGAAGGAG GACGGCTTCA CGGCGCTGCA TCTGGCTGCC 
CTCAACAACC ACCGCGAGGT GGCCCAGATC CTCATCCGGG AGGGCCGCTG TGACGTGAAC 
GTGCGCAACC GGAAGCTGCA GTCCCCGCTG CATCTCGCCG TGCAACAGGC CCACGTGGGG 
CTGGTGCCGC TACTGGTGGA CGCTGGGTGC AGTGTCAACG CCGAGGACGA GGAGGGGGAC 
ACAGCCCTGC ACGTGGCGCT GCAGCGTCAT CAGCTGCTGC CCCTGGTGGC TGATGGGGCC 
GGGGGGGACC CAGGGCCCTT GCAGCTGCTG TCCAGGCTAC AGGCCTCGGG CCTCCCCGGC 
AGCGCGGAGC TGACGGTGGG CGCGGCGGTC GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC 
GTGAGCTACA CCAACCACCG CGGTCGGAGC CCGCTGGACC TGGCCGCCGA GGGTCGCGTG 
CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG 
GCCCCGGGCC CCAGGCAAAC GCTCGGGACC CCCAACACCG TGACGAACCT GCACGTGGGC 
GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG 
CTGTTCTCGC CGTGCCAGCA CCGCACCGTG TGTGAGGAGT GCGCGCGCAG GATGAAGAAG 
TGCATCAGGT GCCAGGTGGT CGTCAGCAAG AAACTGCGCC CAGACGGCTC TGAGGTGGCG 
AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC 
CGGCAGATGG AGGAACGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG 
TTCCAGTGCG GCCACGGCGC ATGCGCCCCC TGCGGCTCCG CGCTCAGCGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_024453318.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309086.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OHu103597
Clone ID Related Accession (Same CDS sequence)	XM_024453318.1
Accession Version	XM_024453318.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGAG CAGCCCCGCC CAACATGGAC CCAGACCCCC AGGCGGGCGT GCAGGTGGGC 
ATGCGGGTGG TGCGCGGCGT GGACTGGAAG TGGGGCCAGC AGGACGGCGG CGAGGGCGGC 
GTGGGCACGG TGGTGGAGCT TGGCCGCCAC GGCAGCCCCT CGACACCCGA CCGCACAGTG 
GTCGTGCAGT GGGACCAGGG CACGCGCACC AACTACCGCG CCGGCTACCA GGGCGCGCAC 
GACCTGCTGC TGTACGACAA CGCCCAGATC GGCGTCCGGC ACCCCAACAT CATCTGTGAC 
TGCTGCAAGA AGCACGGGCT GCGGGGGATG CGCTGGAAGT GCCGTGTGTG CCTGGACTAC 
GACCTCTGCA CGCAGTGCTA CATGCACAAC AAGCATGAGC TCGCCCACGC CTTCGACCGC 
TACGAGACCG CTCACTCGCG CCCTGTCACA CTGAGTCCCC GCCAGGGCCT CCCGAGGATC 
CCACTAAGGG GCATCTTCCA GGGAGCGAAG GTGGTGCGAG GCCCCGACTG GGAGTGGGGC 
TCACAGGATG GAGGGGAAGG GAAACCGGGC CGTGTGGTGG ACATCCGTGG CTGGGATGTG 
GAGACAGGCC GGAGTGTGGC CAGCGTGACG TGGGCTGATG GTACCACCAA TGTGTACCGT 
GTGGGCCACA AGGGCAAGGT GGACCTCAAG TGTGTGGGCG AGGCAGCGGG CGGCTTCTAC 
TACAAGGACC ACCTCCCAAG GCTCGGCAAG CCGGCGGAGC TGCAGCGCAG GGTGAGTGCT 
GACAGCCAGC CCTTCCAGCA CGGGGACAAG GTCAAGTGTC TGCTGGACAC TGATGTCCTG 
CGGGAGATGC AGGAAGGCCA CGGCGGCTGG AACCCCAGGA TGGCGGAGTT TATCGGACAG 
ACGGGCACCG TGCATCGTAT CACGGACCGC GGGGACGTGC GCGTGCAGTT CAACCACGAG 
ACGCGCTGGA CCTTCCACCC CGGGGCGCTC ACCAAGGACA CCAGGAAGGC AGGACAGCTT 
CGTGGGCGGG AGGGAGGCGG CTGGGCTAAG ATGCTCCTGC ACCACTCCTT CTGGGTGGGC 
GACGTGGTCC GGGTCATCGG CGACCTTGAC ACAGTGAAGC GGCTGCAGGC TGGGCATGGC 
GAGTGGACGG ACGACATGGC CCCTGCCCTG GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT 
GGAGACGGGA ACCTGCGTGT AGCAGTCGCT GGTCAGCGGT GGACCTTCAG CCCCTCCTGC 
CTGGTGGCCT ACCGGCCCGA GGAGGATGCC AACCTGGACG TGGCCGAGCG CGCCCGGGAG 
AACAAAAGCT CACTGAGCGT GGCCCTGGAC AAGCTTCGGG CCCAGAAGAG TGACCCAGAG 
CACCCGGGAA GGCTGGTGGT GGAGGTGGCG CTGGGTAACG CAGCCCGGGC TCTGGACCTG 
CTGCGGAGGC GCCCAGAGCA GGTGGACACC AAGAACCAAG GCAGGACCGC TCTGCAAGTG 
GCTGCCTACC TGGGCCAGGT GGAGTTGATA CGGCTGCTGC TACAAGCCAG GGCGGGCGTG 
GACCTGCCGG ACGACGAGGG CAACACGGCA CTGCACTACG CGGCCCTGGG GAACCAGCCC 
GAGGCCACCA GGGTGCTCCT GAGTGCTGGG TGCCGGGCGG ACGCCATCAA CAGCACCCAG 
AGCACAGCAC TGCACGTGGC CGTGCAGAGG GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT 
GAGCGCGGCT GTGACGTCAA CCTGCCCGAC GCCCACTCGG ACACGCCCCT GCACTCCGCC 
ATCTCGGCGG GCACTGGAGC CAGCGGCATT GTCGAGGTCC TCACGGAGGT GCCAAACATC 
GATGTTACCG CCACCAACAG CCAGGGTTTC ACCCTGCTGC ACCATGCCTC CCTCAAGGGT 
CACGCGCTAG CTGTGAGAAA GATTCTGGCT CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG 
GAGGACGGCT TCACGGCGCT GCATCTGGCT GCCCTCAACA ACCACCGCGA GGTGGCCCAG 
ATCCTCATCC GGGAGGGCCG CTGTGACGTG AACGTGCGCA ACCGGAAGCT GCAGTCCCCG 
CTGCATCTCG CCGTGCAACA GGCCCACGTG GGGCTGGTGC CGCTACTGGT GGACGCTGGG 
TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG GACACAGCCC TGCACGTGGC GCTGCAGCGT 
CATCAGCTGC TGCCCCTGGT GGCTGATGGG GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG 
CTGTCCAGGA CATGGGGCGC CGGCTGCTGG GGCTGCCAGG TGCCAGGAGA CGCCTCCCTC 
GGGCCTGCCC CGGCGCCCGC CCTCACCGGC GTCTGTCCTG CCGCCCAGCT ACAGGCCTCG 
GGCCTCCCCG GCAGCGCGGA GCTGACGGTG GGCGCGGCGG TCGCCTGCTT CCTGGCGCTG 
GAGGGCGCCG ACGTGAGCTA CACCAACCAC CGCGGTCGGA GCCCGCTGGA CCTGGCCGCC 
GAGGGTCGCG TGCTCAAGGC CCTTCAGGGC TGCGCCCAGC GCTTCCGGGA GCGGCAGGCG 
GGCGGGGGCG CGGCCCCGGG CCCCAGGCAA ACGCTCGGGA CCCCCAACAC CGTGACGAAC 
CTGCACGTGG GCGCCGCGCC GGGGCCCGAG GCCGCTGAGT GCCTGGTGTG CTCCGAGCTG 
GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACCG TGTGTGAGGG TGAGTGGGGG 
GCCCCGGGGT GGGGAGGCCC GGCTAGTAGG GCCGCAGCCA ACCGCGCTCT CCTCTTCGCA 
GAGTGCGCGC GCAGGATGAA GAAGTGCATC AGGTGCCAGG TGGTCGTCAG CAAGAAACTG 
CGCCCAGACG GCTCTGAGGT GGCGAGCGCC GCCCCCGCCC CCGGCCCGCC GCGCCAGCTG 
GTGGAGGAGC TGCAGAGCCG CTACCGGCAG ATGGAGGAAC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCACG GCGCATGCGC CCCCTGCGGC 
TCCGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT CCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309086.1
CDS	3721..6846
Translation	MSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_024453318.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453318.1

ORF Insert Sequence:

ATGTCCCGAG CAGCCCCGCC CAACATGGAC CCAGACCCCC AGGCGGGCGT GCAGGTGGGC 
ATGCGGGTGG TGCGCGGCGT GGACTGGAAG TGGGGCCAGC AGGACGGCGG CGAGGGCGGC 
GTGGGCACGG TGGTGGAGCT TGGCCGCCAC GGCAGCCCCT CGACACCCGA CCGCACAGTG 
GTCGTGCAGT GGGACCAGGG CACGCGCACC AACTACCGCG CCGGCTACCA GGGCGCGCAC 
GACCTGCTGC TGTACGACAA CGCCCAGATC GGCGTCCGGC ACCCCAACAT CATCTGTGAC 
TGCTGCAAGA AGCACGGGCT GCGGGGGATG CGCTGGAAGT GCCGTGTGTG CCTGGACTAC 
GACCTCTGCA CGCAGTGCTA CATGCACAAC AAGCATGAGC TCGCCCACGC CTTCGACCGC 
TACGAGACCG CTCACTCGCG CCCTGTCACA CTGAGTCCCC GCCAGGGCCT CCCGAGGATC 
CCACTAAGGG GCATCTTCCA GGGAGCGAAG GTGGTGCGAG GCCCCGACTG GGAGTGGGGC 
TCACAGGATG GAGGGGAAGG GAAACCGGGC CGTGTGGTGG ACATCCGTGG CTGGGATGTG 
GAGACAGGCC GGAGTGTGGC CAGCGTGACG TGGGCTGATG GTACCACCAA TGTGTACCGT 
GTGGGCCACA AGGGCAAGGT GGACCTCAAG TGTGTGGGCG AGGCAGCGGG CGGCTTCTAC 
TACAAGGACC ACCTCCCAAG GCTCGGCAAG CCGGCGGAGC TGCAGCGCAG GGTGAGTGCT 
GACAGCCAGC CCTTCCAGCA CGGGGACAAG GTCAAGTGTC TGCTGGACAC TGATGTCCTG 
CGGGAGATGC AGGAAGGCCA CGGCGGCTGG AACCCCAGGA TGGCGGAGTT TATCGGACAG 
ACGGGCACCG TGCATCGTAT CACGGACCGC GGGGACGTGC GCGTGCAGTT CAACCACGAG 
ACGCGCTGGA CCTTCCACCC CGGGGCGCTC ACCAAGGACA CCAGGAAGGC AGGACAGCTT 
CGTGGGCGGG AGGGAGGCGG CTGGGCTAAG ATGCTCCTGC ACCACTCCTT CTGGGTGGGC 
GACGTGGTCC GGGTCATCGG CGACCTTGAC ACAGTGAAGC GGCTGCAGGC TGGGCATGGC 
GAGTGGACGG ACGACATGGC CCCTGCCCTG GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT 
GGAGACGGGA ACCTGCGTGT AGCAGTCGCT GGTCAGCGGT GGACCTTCAG CCCCTCCTGC 
CTGGTGGCCT ACCGGCCCGA GGAGGATGCC AACCTGGACG TGGCCGAGCG CGCCCGGGAG 
AACAAAAGCT CACTGAGCGT GGCCCTGGAC AAGCTTCGGG CCCAGAAGAG TGACCCAGAG 
CACCCGGGAA GGCTGGTGGT GGAGGTGGCG CTGGGTAACG CAGCCCGGGC TCTGGACCTG 
CTGCGGAGGC GCCCAGAGCA GGTGGACACC AAGAACCAAG GCAGGACCGC TCTGCAAGTG 
GCTGCCTACC TGGGCCAGGT GGAGTTGATA CGGCTGCTGC TACAAGCCAG GGCGGGCGTG 
GACCTGCCGG ACGACGAGGG CAACACGGCA CTGCACTACG CGGCCCTGGG GAACCAGCCC 
GAGGCCACCA GGGTGCTCCT GAGTGCTGGG TGCCGGGCGG ACGCCATCAA CAGCACCCAG 
AGCACAGCAC TGCACGTGGC CGTGCAGAGG GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT 
GAGCGCGGCT GTGACGTCAA CCTGCCCGAC GCCCACTCGG ACACGCCCCT GCACTCCGCC 
ATCTCGGCGG GCACTGGAGC CAGCGGCATT GTCGAGGTCC TCACGGAGGT GCCAAACATC 
GATGTTACCG CCACCAACAG CCAGGGTTTC ACCCTGCTGC ACCATGCCTC CCTCAAGGGT 
CACGCGCTAG CTGTGAGAAA GATTCTGGCT CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG 
GAGGACGGCT TCACGGCGCT GCATCTGGCT GCCCTCAACA ACCACCGCGA GGTGGCCCAG 
ATCCTCATCC GGGAGGGCCG CTGTGACGTG AACGTGCGCA ACCGGAAGCT GCAGTCCCCG 
CTGCATCTCG CCGTGCAACA GGCCCACGTG GGGCTGGTGC CGCTACTGGT GGACGCTGGG 
TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG GACACAGCCC TGCACGTGGC GCTGCAGCGT 
CATCAGCTGC TGCCCCTGGT GGCTGATGGG GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG 
CTGTCCAGGA CATGGGGCGC CGGCTGCTGG GGCTGCCAGG TGCCAGGAGA CGCCTCCCTC 
GGGCCTGCCC CGGCGCCCGC CCTCACCGGC GTCTGTCCTG CCGCCCAGCT ACAGGCCTCG 
GGCCTCCCCG GCAGCGCGGA GCTGACGGTG GGCGCGGCGG TCGCCTGCTT CCTGGCGCTG 
GAGGGCGCCG ACGTGAGCTA CACCAACCAC CGCGGTCGGA GCCCGCTGGA CCTGGCCGCC 
GAGGGTCGCG TGCTCAAGGC CCTTCAGGGC TGCGCCCAGC GCTTCCGGGA GCGGCAGGCG 
GGCGGGGGCG CGGCCCCGGG CCCCAGGCAA ACGCTCGGGA CCCCCAACAC CGTGACGAAC 
CTGCACGTGG GCGCCGCGCC GGGGCCCGAG GCCGCTGAGT GCCTGGTGTG CTCCGAGCTG 
GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACCG TGTGTGAGGG TGAGTGGGGG 
GCCCCGGGGT GGGGAGGCCC GGCTAGTAGG GCCGCAGCCA ACCGCGCTCT CCTCTTCGCA 
GAGTGCGCGC GCAGGATGAA GAAGTGCATC AGGTGCCAGG TGGTCGTCAG CAAGAAACTG 
CGCCCAGACG GCTCTGAGGT GGCGAGCGCC GCCCCCGCCC CCGGCCCGCC GCGCCAGCTG 
GTGGAGGAGC TGCAGAGCCG CTACCGGCAG ATGGAGGAAC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCACG GCGCATGCGC CCCCTGCGGC 
TCCGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT CCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_024453319.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309087.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X25, mRNA.

Price & Availability↑

CloneID	OHu103598
Clone ID Related Accession (Same CDS sequence)	XM_024453319.1
Accession Version	XM_024453319.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAGGC GGGCGTGCAG GTGGGCATGC GGGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTTGGC 
CGCCACGGCA GCCCCTCGAC ACCCGACCGC ACAGTGGTCG TGCAGTGGGA CCAGGGCACG 
CGCACCAACT ACCGCGCCGG CTACCAGGGC GCGCACGACC TGCTGCTGTA CGACAACGCC 
CAGATCGGCG TCCGGCACCC CAACATCATC TGTGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGGATGCGCT GGAAGTGCCG TGTGTGCCTG GACTACGACC TCTGCACGCA GTGCTACATG 
CACAACAAGC ATGAGCTCGC CCACGCCTTC GACCGCTACG AGACCGCTCA CTCGCGCCCT 
GTCACACTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAC TAAGGGGCAT CTTCCAGGGA 
GCGAAGGTGG TGCGAGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG GGAAGGGAAA 
CCGGGCCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGGAG TGTGGCCAGC 
GTGACGTGGG CTGATGGTAC CACCAATGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCGGGCGGC TTCTACTACA AGGACCACCT CCCAAGGCTC 
GGCAAGCCGG CGGAGCTGCA GCGCAGGGTG AGTGCTGACA GCCAGCCCTT CCAGCACGGG 
GACAAGGTCA AGTGTCTGCT GGACACTGAT GTCCTGCGGG AGATGCAGGA AGGCCACGGC 
GGCTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA TCGTATCACG 
GACCGCGGGG ACGTGCGCGT GCAGTTCAAC CACGAGACGC GCTGGACCTT CCACCCCGGG 
GCGCTCACCA AGCACCACTC CTTCTGGGTG GGCGACGTGG TCCGGGTCAT CGGCGACCTT 
GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CGGACGACAT GGCCCCTGCC 
CTGGGCCGCG TCGGGAAGGT GGTGAAAGTG TTTGGAGACG GGAACCTGCG TGTAGCAGTC 
GCTGGTCAGC GGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC CGAGGAGGAT 
GCCAACCTGG ACGTGGCCGA GCGCGCCCGG GAGAACAAAA GCTCACTGAG CGTGGCCCTG 
GACAAGCTTC GGGCCCAGAA GAGTGACCCA GAGCACCCGG GAAGGCTGGT GGTGGAGGTG 
GCGCTGGGTA ACGCAGCCCG GGCTCTGGAC CTGCTGCGGA GGCGCCCAGA GCAGGTGGAC 
ACCAAGAACC AAGGCAGGAC CGCTCTGCAA GTGGCTGCCT ACCTGGGCCA GGTGGAGTTG 
ATACGGCTGC TGCTACAAGC CAGGGCGGGC GTGGACCTGC CGGACGACGA GGGCAACACG 
GCACTGCACT ACGCGGCCCT GGGGAACCAG CCCGAGGCCA CCAGGGTGCT CCTGAGTGCT 
GGGTGCCGGG CGGACGCCAT CAACAGCACC CAGAGCACAG CACTGCACGT GGCCGTGCAG 
AGGGGCTTCC TGGAGGTGGT GCGGGCCCTG TGTGAGCGCG GCTGTGACGT CAACCTGCCC 
GACGCCCACT CGGACACGCC CCTGCACTCC GCCATCTCGG CGGGCACTGG AGCCAGCGGC 
ATTGTCGAGG TCCTCACGGA GGTGCCAAAC ATCGATGTTA CCGCCACCAA CAGCCAGGGT 
TTCACCCTGC TGCACCATGC CTCCCTCAAG GGTCACGCGC TAGCTGTGAG AAAGATTCTG 
GCTCGGGCGC GGCAGCTGGT GGACGCCAAG AAGGAGGACG GCTTCACGGC GCTGCATCTG 
GCTGCCCTCA ACAACCACCG CGAGGTGGCC CAGATCCTCA TCCGGGAGGG CCGCTGTGAC 
GTGAACGTGC GCAACCGGAA GCTGCAGTCC CCGCTGCATC TCGCCGTGCA ACAGGCCCAC 
GTGGGGCTGG TGCCGCTACT GGTGGACGCT GGGTGCAGTG TCAACGCCGA GGACGAGGAG 
GGGGACACAG CCCTGCACGT GGCGCTGCAG CGTCATCAGC TGCTGCCCCT GGTGGCTGAT 
GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGACATGGGG CGCCGGCTGC 
TGGGGCTGCC AGGTGCCAGG AGACGCCTCC CTCGGGCCTG CCCCGGCGCC CGCCCTCACC 
GGCGTCTGTC CTGCCGCCCA GCTACAGGCC TCGGGCCTCC CCGGCAGCGC GGAGCTGACG 
GTGGGCGCGG CGGTCGCCTG CTTCCTGGCG CTGGAGGGCG CCGACGTGAG CTACACCAAC 
CACCGCGGTC GGAGCCCGCT GGACCTGGCC GCCGAGGGTC GCGTGCTCAA GGCCCTTCAG 
GGCTGCGCCC AGCGCTTCCG GGAGCGGCAG GCGGGCGGGG GCGCGGCCCC GGGCCCCAGG 
CAAACGCTCG GGACCCCCAA CACCGTGACG AACCTGCACG TGGGCGCCGC GCCGGGGCCC 
GAGGCCGCTG AGTGCCTGGT GTGCTCCGAG CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC 
CAGCACCGCA CCGTGTGTGA GGGTGAGTGG GGGGCCCCGG GGTGGGGAGG CCCGGCTAGT 
AGGGCCGCAG CCAACCGCGC TCTCCTCTTC GCAGAGTGCG CGCGCAGGAT GAAGAAGTGC 
ATCAGGTGCC AGGTGGTCGT CAGCAAGAAA CTGCGCCCAG ACGGCTCTGA GGTGGCGAGC 
GCCGCCCCCG CCCCCGGCCC GCCGCGCCAG CTGGTGGAGG AGCTGCAGAG CCGCTACCGG 
CAGATGGAGG AACGCATCAC CTGCCCCATC TGCATCGACA GCCACATCCG CCTCGTGTTC 
CAGTGCGGCC ACGGCGCATG CGCCCCCTGC GGCTCCGCGC TCAGCGCCTG CCCCATCTGC 
CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309087.1
CDS	366..3404
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_024453319.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453319.1

ORF Insert Sequence:

ATGGACCCAG ACCCCCAGGC GGGCGTGCAG GTGGGCATGC GGGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTTGGC 
CGCCACGGCA GCCCCTCGAC ACCCGACCGC ACAGTGGTCG TGCAGTGGGA CCAGGGCACG 
CGCACCAACT ACCGCGCCGG CTACCAGGGC GCGCACGACC TGCTGCTGTA CGACAACGCC 
CAGATCGGCG TCCGGCACCC CAACATCATC TGTGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGGATGCGCT GGAAGTGCCG TGTGTGCCTG GACTACGACC TCTGCACGCA GTGCTACATG 
CACAACAAGC ATGAGCTCGC CCACGCCTTC GACCGCTACG AGACCGCTCA CTCGCGCCCT 
GTCACACTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAC TAAGGGGCAT CTTCCAGGGA 
GCGAAGGTGG TGCGAGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG GGAAGGGAAA 
CCGGGCCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGGAG TGTGGCCAGC 
GTGACGTGGG CTGATGGTAC CACCAATGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCGGGCGGC TTCTACTACA AGGACCACCT CCCAAGGCTC 
GGCAAGCCGG CGGAGCTGCA GCGCAGGGTG AGTGCTGACA GCCAGCCCTT CCAGCACGGG 
GACAAGGTCA AGTGTCTGCT GGACACTGAT GTCCTGCGGG AGATGCAGGA AGGCCACGGC 
GGCTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA TCGTATCACG 
GACCGCGGGG ACGTGCGCGT GCAGTTCAAC CACGAGACGC GCTGGACCTT CCACCCCGGG 
GCGCTCACCA AGCACCACTC CTTCTGGGTG GGCGACGTGG TCCGGGTCAT CGGCGACCTT 
GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CGGACGACAT GGCCCCTGCC 
CTGGGCCGCG TCGGGAAGGT GGTGAAAGTG TTTGGAGACG GGAACCTGCG TGTAGCAGTC 
GCTGGTCAGC GGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC CGAGGAGGAT 
GCCAACCTGG ACGTGGCCGA GCGCGCCCGG GAGAACAAAA GCTCACTGAG CGTGGCCCTG 
GACAAGCTTC GGGCCCAGAA GAGTGACCCA GAGCACCCGG GAAGGCTGGT GGTGGAGGTG 
GCGCTGGGTA ACGCAGCCCG GGCTCTGGAC CTGCTGCGGA GGCGCCCAGA GCAGGTGGAC 
ACCAAGAACC AAGGCAGGAC CGCTCTGCAA GTGGCTGCCT ACCTGGGCCA GGTGGAGTTG 
ATACGGCTGC TGCTACAAGC CAGGGCGGGC GTGGACCTGC CGGACGACGA GGGCAACACG 
GCACTGCACT ACGCGGCCCT GGGGAACCAG CCCGAGGCCA CCAGGGTGCT CCTGAGTGCT 
GGGTGCCGGG CGGACGCCAT CAACAGCACC CAGAGCACAG CACTGCACGT GGCCGTGCAG 
AGGGGCTTCC TGGAGGTGGT GCGGGCCCTG TGTGAGCGCG GCTGTGACGT CAACCTGCCC 
GACGCCCACT CGGACACGCC CCTGCACTCC GCCATCTCGG CGGGCACTGG AGCCAGCGGC 
ATTGTCGAGG TCCTCACGGA GGTGCCAAAC ATCGATGTTA CCGCCACCAA CAGCCAGGGT 
TTCACCCTGC TGCACCATGC CTCCCTCAAG GGTCACGCGC TAGCTGTGAG AAAGATTCTG 
GCTCGGGCGC GGCAGCTGGT GGACGCCAAG AAGGAGGACG GCTTCACGGC GCTGCATCTG 
GCTGCCCTCA ACAACCACCG CGAGGTGGCC CAGATCCTCA TCCGGGAGGG CCGCTGTGAC 
GTGAACGTGC GCAACCGGAA GCTGCAGTCC CCGCTGCATC TCGCCGTGCA ACAGGCCCAC 
GTGGGGCTGG TGCCGCTACT GGTGGACGCT GGGTGCAGTG TCAACGCCGA GGACGAGGAG 
GGGGACACAG CCCTGCACGT GGCGCTGCAG CGTCATCAGC TGCTGCCCCT GGTGGCTGAT 
GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGACATGGGG CGCCGGCTGC 
TGGGGCTGCC AGGTGCCAGG AGACGCCTCC CTCGGGCCTG CCCCGGCGCC CGCCCTCACC 
GGCGTCTGTC CTGCCGCCCA GCTACAGGCC TCGGGCCTCC CCGGCAGCGC GGAGCTGACG 
GTGGGCGCGG CGGTCGCCTG CTTCCTGGCG CTGGAGGGCG CCGACGTGAG CTACACCAAC 
CACCGCGGTC GGAGCCCGCT GGACCTGGCC GCCGAGGGTC GCGTGCTCAA GGCCCTTCAG 
GGCTGCGCCC AGCGCTTCCG GGAGCGGCAG GCGGGCGGGG GCGCGGCCCC GGGCCCCAGG 
CAAACGCTCG GGACCCCCAA CACCGTGACG AACCTGCACG TGGGCGCCGC GCCGGGGCCC 
GAGGCCGCTG AGTGCCTGGT GTGCTCCGAG CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC 
CAGCACCGCA CCGTGTGTGA GGGTGAGTGG GGGGCCCCGG GGTGGGGAGG CCCGGCTAGT 
AGGGCCGCAG CCAACCGCGC TCTCCTCTTC GCAGAGTGCG CGCGCAGGAT GAAGAAGTGC 
ATCAGGTGCC AGGTGGTCGT CAGCAAGAAA CTGCGCCCAG ACGGCTCTGA GGTGGCGAGC 
GCCGCCCCCG CCCCCGGCCC GCCGCGCCAG CTGGTGGAGG AGCTGCAGAG CCGCTACCGG 
CAGATGGAGG AACGCATCAC CTGCCCCATC TGCATCGACA GCCACATCCG CCTCGTGTTC 
CAGTGCGGCC ACGGCGCATG CGCCCCCTGC GGCTCCGCGC TCAGCGCCTG CCCCATCTGC 
CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( NM_001170688.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001164159.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 4, mRNA.

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CloneID	OHu01261
Clone ID Related Accession (Same CDS sequence)	NM_001170688.1
Accession Version	NM_001170688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3018bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL691432.54, DC402226.1, AB076692.1 and BC037542.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB076692.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2152798 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGCAAGCCGG CGGAGCTGCA GCGCAGGGTG 
AGTGCTGACA GCCAGCCCTT CCAGCACGGG GACAAGGTCA AGTGTCTGCT GGACACTGAT 
GTCCTGCGGG AGATGCAGGA AGGCCACGGC GGCTGGAACC CCAGGATGGC GGAGTTTATC 
GGACAGACGG GCACCGTGCA TCGTATCACG GACCGCGGGG ACGTGCGCGT GCAGTTCAAC 
CACGAGACGC GCTGGACCTT CCACCCCGGG GCGCTCACCA AGCACCACTC CTTCTGGGTG 
GGCGACGTGG TCCGGGTCAT CGGCGACCTT GACACAGTGA AGCGGCTGCA GGCTGGGCAT 
GGCGAGTGGA CGGACGACAT GGCCCCTGCC CTGGGCCGCG TCGGGAAGGT GGTGAAAGTG 
TTTGGAGACG GGAACCTGCG TGTAGCAGTC GCTGGTCAGC GGTGGACCTT CAGCCCCTCC 
TGCCTGGTGG CCTACCGGCC CGAGGAGGAT GCCAACCTGG ACGTGGCCGA GCGCGCCCGG 
GAGAACAAAA GCTCACTGAG CGTGGCCCTG GACAAGCTTC GGGCCCAGAA GAGTGACCCA 
GAGCACCCGG GAAGGCTGGT GGTGGAGGTG GCGCTGGGTA ACGCAGCCCG GGCTCTGGAC 
CTGCTGCGGA GGCGCCCAGA GCAGGTGGAC ACCAAGAACC AAGGCAGGAC CGCTCTGCAA 
GTGGCTGCCT ACCTGGGCCA GGTGGAGTTG ATACGGCTGC TGCTACAAGC CAGGGCGGGC 
GTGGACCTGC CGGACGACGA GGGCAACACG GCACTGCACT ACGCGGCCCT GGGGAACCAG 
CCCGAGGCCA CCAGGGTGCT CCTGAGTGCT GGGTGCCGGG CGGACGCCAT CAACAGCACC 
CAGAGCACAG CACTGCACGT GGCCGTGCAG AGGGGCTTCC TGGAGGTGGT GCGGGCCCTG 
TGTGAGCGCG GCTGTGACGT CAACCTGCCC GACGCCCACT CGGACACGCC CCTGCACTCC 
GCCATCTCGG CGGGCACTGG AGCCAGCGGC ATTGTCGAGG TCCTCACGGA GGTGCCAAAC 
ATCGATGTTA CCGCCACCAA CAGCCAGGGT TTCACCCTGC TGCACCATGC CTCCCTCAAG 
GGTCACGCGC TAGCTGTGAG AAAGATTCTG GCTCGGGCGC GGCAGCTGGT GGACGCCAAG 
AAGGAGGACG GCTTCACGGC GCTGCATCTG GCTGCCCTCA ACAACCACCG CGAGGTGGCC 
CAGATCCTCA TCCGGGAGGG CCGCTGTGAC GTGAACGTGC GCAACCGGAA GCTGCAGTCC 
CCGCTGCATC TCGCCGTGCA ACAGGCCCAC GTGGGGCTGG TGCCGCTACT GGTGGACGCT 
GGGTGCAGTG TCAACGCCGA GGACGAGGAG GGGGACACAG CCCTGCACGT GGCGCTGCAG 
CGTCATCAGC TGCTGCCCCT GGTGGCTGAT GGGGCCGGGG GGGACCCAGG GCCCTTGCAG 
CTGCTGTCCA GGCTACAGGC CTCGGGCCTC CCCGGCAGCG CGGAGCTGAC GGTGGGCGCG 
GCGGTCGCCT GCTTCCTGGC GCTGGAGGGC GCCGACGTGA GCTACACCAA CCACCGCGGT 
CGGAGCCCGC TGGACCTGGC CGCCGAGGGT CGCGTGCTCA AGGCCCTTCA GGGCTGCGCC 
CAGCGCTTCC GGGAGCGGCA GGCGGGCGGG GGCGCGGCCC CGGGCCCCAG GCAAACGCTC 
GGGACCCCCA ACACCGTGAC GAACCTGCAC GTGGGCGCCG CGCCGGGGCC CGAGGCCGCT 
GAGTGCCTGG TGTGCTCCGA GCTGGCGCTG CTGGTGCTGT TCTCGCCGTG CCAGCACCGC 
ACCGTGTGTG AGGAGTGCGC GCGCAGGATG AAGAAGTGCA TCAGGTGCCA GGTGGTCGTC 
AGCAAGAAAC TGCGCCCAGA CGGCTCTGAG GTGGCGAGCG CCGCCCCCGC CCCCGGCCCG 
CCGCGCCAGC TGGTGGAGGA GCTGCAGAGC CGCTACCGGC AGATGGAGGA ACGCATCACC 
TGCCCCATCT GCATCGACAG CCACATCCGC CTCGTGTTCC AGTGCGGCCA CGGCGCATGC 
GCCCCCTGCG GCTCCGCGCT CAGCGCCTGC CCCATCTGCC GCCAGCCCAT CCGCGACCGC 
ATCCAGATCT TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164159.1
CDS	46..3063
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAARPTMDPSAHRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_001170688.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170688.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGCAAGCCGG CGGAGCTGCA GCGCAGGGTG 
AGTGCTGACA GCCAGCCCTT CCAGCACGGG GACAAGGTCA AGTGTCTGCT GGACACTGAT 
GTCCTGCGGG AGATGCAGGA AGGCCACGGC GGCTGGAACC CCAGGATGGC GGAGTTTATC 
GGACAGACGG GCACCGTGCA TCGTATCACG GACCGCGGGG ACGTGCGCGT GCAGTTCAAC 
CACGAGACGC GCTGGACCTT CCACCCCGGG GCGCTCACCA AGCACCACTC CTTCTGGGTG 
GGCGACGTGG TCCGGGTCAT CGGCGACCTT GACACAGTGA AGCGGCTGCA GGCTGGGCAT 
GGCGAGTGGA CGGACGACAT GGCCCCTGCC CTGGGCCGCG TCGGGAAGGT GGTGAAAGTG 
TTTGGAGACG GGAACCTGCG TGTAGCAGTC GCTGGTCAGC GGTGGACCTT CAGCCCCTCC 
TGCCTGGTGG CCTACCGGCC CGAGGAGGAT GCCAACCTGG ACGTGGCCGA GCGCGCCCGG 
GAGAACAAAA GCTCACTGAG CGTGGCCCTG GACAAGCTTC GGGCCCAGAA GAGTGACCCA 
GAGCACCCGG GAAGGCTGGT GGTGGAGGTG GCGCTGGGTA ACGCAGCCCG GGCTCTGGAC 
CTGCTGCGGA GGCGCCCAGA GCAGGTGGAC ACCAAGAACC AAGGCAGGAC CGCTCTGCAA 
GTGGCTGCCT ACCTGGGCCA GGTGGAGTTG ATACGGCTGC TGCTACAAGC CAGGGCGGGC 
GTGGACCTGC CGGACGACGA GGGCAACACG GCACTGCACT ACGCGGCCCT GGGGAACCAG 
CCCGAGGCCA CCAGGGTGCT CCTGAGTGCT GGGTGCCGGG CGGACGCCAT CAACAGCACC 
CAGAGCACAG CACTGCACGT GGCCGTGCAG AGGGGCTTCC TGGAGGTGGT GCGGGCCCTG 
TGTGAGCGCG GCTGTGACGT CAACCTGCCC GACGCCCACT CGGACACGCC CCTGCACTCC 
GCCATCTCGG CGGGCACTGG AGCCAGCGGC ATTGTCGAGG TCCTCACGGA GGTGCCAAAC 
ATCGATGTTA CCGCCACCAA CAGCCAGGGT TTCACCCTGC TGCACCATGC CTCCCTCAAG 
GGTCACGCGC TAGCTGTGAG AAAGATTCTG GCTCGGGCGC GGCAGCTGGT GGACGCCAAG 
AAGGAGGACG GCTTCACGGC GCTGCATCTG GCTGCCCTCA ACAACCACCG CGAGGTGGCC 
CAGATCCTCA TCCGGGAGGG CCGCTGTGAC GTGAACGTGC GCAACCGGAA GCTGCAGTCC 
CCGCTGCATC TCGCCGTGCA ACAGGCCCAC GTGGGGCTGG TGCCGCTACT GGTGGACGCT 
GGGTGCAGTG TCAACGCCGA GGACGAGGAG GGGGACACAG CCCTGCACGT GGCGCTGCAG 
CGTCATCAGC TGCTGCCCCT GGTGGCTGAT GGGGCCGGGG GGGACCCAGG GCCCTTGCAG 
CTGCTGTCCA GGCTACAGGC CTCGGGCCTC CCCGGCAGCG CGGAGCTGAC GGTGGGCGCG 
GCGGTCGCCT GCTTCCTGGC GCTGGAGGGC GCCGACGTGA GCTACACCAA CCACCGCGGT 
CGGAGCCCGC TGGACCTGGC CGCCGAGGGT CGCGTGCTCA AGGCCCTTCA GGGCTGCGCC 
CAGCGCTTCC GGGAGCGGCA GGCGGGCGGG GGCGCGGCCC CGGGCCCCAG GCAAACGCTC 
GGGACCCCCA ACACCGTGAC GAACCTGCAC GTGGGCGCCG CGCCGGGGCC CGAGGCCGCT 
GAGTGCCTGG TGTGCTCCGA GCTGGCGCTG CTGGTGCTGT TCTCGCCGTG CCAGCACCGC 
ACCGTGTGTG AGGAGTGCGC GCGCAGGATG AAGAAGTGCA TCAGGTGCCA GGTGGTCGTC 
AGCAAGAAAC TGCGCCCAGA CGGCTCTGAG GTGGCGAGCG CCGCCCCCGC CCCCGGCCCG 
CCGCGCCAGC TGGTGGAGGA GCTGCAGAGC CGCTACCGGC AGATGGAGGA ACGCATCACC 
TGCCCCATCT GCATCGACAG CCACATCCGC CTCGTGTTCC AGTGCGGCCA CGGCGCATGC 
GCCCCCTGCG GCTCCGCGCT CAGCGCCTGC CCCATCTGCC GCCAGCCCAT CCGCGACCGC 
ATCCAGATCT TCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( NM_001170687.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001164158.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu01520
Clone ID Related Accession (Same CDS sequence)	NM_001170687.1
Accession Version	NM_001170687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3171bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL691432.54, AK302757.1, AB076691.1, BC016490.1 and BC037542.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (3) that is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC037542.1, AK302757.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCCGCCC 
AACATGGACC CAGACCCCCA GGCGGGCGTG CAGGTGGGCA TGCGGGTGGT GCGCGGCGTG 
GACTGGAAGT GGGGCCAGCA GGACGGCGGC GAGGGCGGCG TGGGCACGGT GGTGGAGCTT 
GGCCGCCACG GCAGCCCCTC GACACCCGAC CGCACAGTGG TCGTGCAGTG GGACCAGGGC 
ACGCGCACCA ACTACCGCGC CGGCTACCAG GGCGCGCACG ACCTGCTGCT GTACGACAAC 
GCCCAGATCG GCGTCCGGCA CCCCAACATC ATCTGTGACT GCTGCAAGAA GCACGGGCTG 
CGGGGGATGC GCTGGAAGTG CCGTGTGTGC CTGGACTACG ACCTCTGCAC GCAGTGCTAC 
ATGCACAACA AGCATGAGCT CGCCCACGCC TTCGACCGCT ACGAGACCGC TCACTCGCGC 
CCTGTCACAC TGAGTCCCCG CCAGGGCCTC CCGAGGATCC CACTAAGGGG CATCTTCCAG 
GGAGCGAAGG TGGTGCGAGG CCCCGACTGG GAGTGGGGCT CACAGGATGG AGGGGAAGGG 
AAACCGGGCC GTGTGGTGGA CATCCGTGGC TGGGATGTGG AGACAGGCCG GAGTGTGGCC 
AGCGTGACGT GGGCTGATGG TACCACCAAT GTGTACCGTG TGGGCCACAA GGGCAAGGTG 
GACCTCAAGT GTGTGGGCGA GGCAGCGGGC GGCTTCTACT ACAAGGACCA CCTCCCAAGG 
CTCGGCAAGC CGGCGGAGCT GCAGCGCAGG GTGAGTGCTG ACAGCCAGCC CTTCCAGCAC 
GGGGACAAGG TCAAGTGTCT GCTGGACACT GATGTCCTGC GGGAGATGCA GGAAGGCCAC 
GGCGGCTGGA ACCCCAGGAT GGCGGAGTTT ATCGGACAGA CGGGCACCGT GCATCGTATC 
ACGGACCGCG GGGACGTGCG CGTGCAGTTC AACCACGAGA CGCGCTGGAC CTTCCACCCC 
GGGGCGCTCA CCAAGCACCA CTCCTTCTGG GTGGGCGACG TGGTCCGGGT CATCGGCGAC 
CTTGACACAG TGAAGCGGCT GCAGGCTGGG CATGGCGAGT GGACGGACGA CATGGCCCCT 
GCCCTGGGCC GCGTCGGGAA GGTGGTGAAA GTGTTTGGAG ACGGGAACCT GCGTGTAGCA 
GTCGCTGGTC AGCGGTGGAC CTTCAGCCCC TCCTGCCTGG TGGCCTACCG GCCCGAGGAG 
GATGCCAACC TGGACGTGGC CGAGCGCGCC CGGGAGAACA AAAGCTCACT GAGCGTGGCC 
CTGGACAAGC TTCGGGCCCA GAAGAGTGAC CCAGAGCACC CGGGAAGGCT GGTGGTGGAG 
GTGGCGCTGG GTAACGCAGC CCGGGCTCTG GACCTGCTGC GGAGGCGCCC AGAGCAGGTG 
GACACCAAGA ACCAAGGCAG GACCGCTCTG CAAGTGGCTG CCTACCTGGG CCAGGTGGAG 
TTGATACGGC TGCTGCTACA AGCCAGGGCG GGCGTGGACC TGCCGGACGA CGAGGGCAAC 
ACGGCACTGC ACTACGCGGC CCTGGGGAAC CAGCCCGAGG CCACCAGGGT GCTCCTGAGT 
GCTGGGTGCC GGGCGGACGC CATCAACAGC ACCCAGAGCA CAGCACTGCA CGTGGCCGTG 
CAGAGGGGCT TCCTGGAGGT GGTGCGGGCC CTGTGTGAGC GCGGCTGTGA CGTCAACCTG 
CCCGACGCCC ACTCGGACAC GCCCCTGCAC TCCGCCATCT CGGCGGGCAC TGGAGCCAGC 
GGCATTGTCG AGGTCCTCAC GGAGGTGCCA AACATCGATG TTACCGCCAC CAACAGCCAG 
GGTTTCACCC TGCTGCACCA TGCCTCCCTC AAGGGTCACG CGCTAGCTGT GAGAAAGATT 
CTGGCTCGGG CGCGGCAGCT GGTGGACGCC AAGAAGGAGG ACGGCTTCAC GGCGCTGCAT 
CTGGCTGCCC TCAACAACCA CCGCGAGGTG GCCCAGATCC TCATCCGGGA GGGCCGCTGT 
GACGTGAACG TGCGCAACCG GAAGCTGCAG TCCCCGCTGC ATCTCGCCGT GCAACAGGCC 
CACGTGGGGC TGGTGCCGCT ACTGGTGGAC GCTGGGTGCA GTGTCAACGC CGAGGACGAG 
GAGGGGGACA CAGCCCTGCA CGTGGCGCTG CAGCGTCATC AGCTGCTGCC CCTGGTGGCT 
GATGGGGCCG GGGGGGACCC AGGGCCCTTG CAGCTGCTGT CCAGGCTACA GGCCTCGGGC 
CTCCCCGGCA GCGCGGAGCT GACGGTGGGC GCGGCGGTCG CCTGCTTCCT GGCGCTGGAG 
GGCGCCGACG TGAGCTACAC CAACCACCGC GGTCGGAGCC CGCTGGACCT GGCCGCCGAG 
GGTCGCGTGC TCAAGGCCCT TCAGGGCTGC GCCCAGCGCT TCCGGGAGCG GCAGGCGGGC 
GGGGGCGCGG CCCCGGGCCC CAGGCAAACG CTCGGGACCC CCAACACCGT GACGAACCTG 
CACGTGGGCG CCGCGCCGGG GCCCGAGGCC GCTGAGTGCC TGGTGTGCTC CGAGCTGGCG 
CTGCTGGTGC TGTTCTCGCC GTGCCAGCAC CGCACCGTGT GTGAGGAGTG CGCGCGCAGG 
ATGAAGAAGT GCATCAGGTG CCAGGTGGTC GTCAGCAAGA AACTGCGCCC AGACGGCTCT 
GAGGTGGCGA GCGCCGCCCC CGCCCCCGGC CCGCCGCGCC AGCTGGTGGA GGAGCTGCAG 
AGCCGCTACC GGCAGATGGA GGAACGCATC ACCTGCCCCA TCTGCATCGA CAGCCACATC 
CGCCTCGTGT TCCAGTGCGG CCACGGCGCA TGCGCCCCCT GCGGCTCCGC GCTCAGCGCC 
TGCCCCATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164158.1
CDS	46..3216
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_001170687.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170687.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCCGCCC 
AACATGGACC CAGACCCCCA GGCGGGCGTG CAGGTGGGCA TGCGGGTGGT GCGCGGCGTG 
GACTGGAAGT GGGGCCAGCA GGACGGCGGC GAGGGCGGCG TGGGCACGGT GGTGGAGCTT 
GGCCGCCACG GCAGCCCCTC GACACCCGAC CGCACAGTGG TCGTGCAGTG GGACCAGGGC 
ACGCGCACCA ACTACCGCGC CGGCTACCAG GGCGCGCACG ACCTGCTGCT GTACGACAAC 
GCCCAGATCG GCGTCCGGCA CCCCAACATC ATCTGTGACT GCTGCAAGAA GCACGGGCTG 
CGGGGGATGC GCTGGAAGTG CCGTGTGTGC CTGGACTACG ACCTCTGCAC GCAGTGCTAC 
ATGCACAACA AGCATGAGCT CGCCCACGCC TTCGACCGCT ACGAGACCGC TCACTCGCGC 
CCTGTCACAC TGAGTCCCCG CCAGGGCCTC CCGAGGATCC CACTAAGGGG CATCTTCCAG 
GGAGCGAAGG TGGTGCGAGG CCCCGACTGG GAGTGGGGCT CACAGGATGG AGGGGAAGGG 
AAACCGGGCC GTGTGGTGGA CATCCGTGGC TGGGATGTGG AGACAGGCCG GAGTGTGGCC 
AGCGTGACGT GGGCTGATGG TACCACCAAT GTGTACCGTG TGGGCCACAA GGGCAAGGTG 
GACCTCAAGT GTGTGGGCGA GGCAGCGGGC GGCTTCTACT ACAAGGACCA CCTCCCAAGG 
CTCGGCAAGC CGGCGGAGCT GCAGCGCAGG GTGAGTGCTG ACAGCCAGCC CTTCCAGCAC 
GGGGACAAGG TCAAGTGTCT GCTGGACACT GATGTCCTGC GGGAGATGCA GGAAGGCCAC 
GGCGGCTGGA ACCCCAGGAT GGCGGAGTTT ATCGGACAGA CGGGCACCGT GCATCGTATC 
ACGGACCGCG GGGACGTGCG CGTGCAGTTC AACCACGAGA CGCGCTGGAC CTTCCACCCC 
GGGGCGCTCA CCAAGCACCA CTCCTTCTGG GTGGGCGACG TGGTCCGGGT CATCGGCGAC 
CTTGACACAG TGAAGCGGCT GCAGGCTGGG CATGGCGAGT GGACGGACGA CATGGCCCCT 
GCCCTGGGCC GCGTCGGGAA GGTGGTGAAA GTGTTTGGAG ACGGGAACCT GCGTGTAGCA 
GTCGCTGGTC AGCGGTGGAC CTTCAGCCCC TCCTGCCTGG TGGCCTACCG GCCCGAGGAG 
GATGCCAACC TGGACGTGGC CGAGCGCGCC CGGGAGAACA AAAGCTCACT GAGCGTGGCC 
CTGGACAAGC TTCGGGCCCA GAAGAGTGAC CCAGAGCACC CGGGAAGGCT GGTGGTGGAG 
GTGGCGCTGG GTAACGCAGC CCGGGCTCTG GACCTGCTGC GGAGGCGCCC AGAGCAGGTG 
GACACCAAGA ACCAAGGCAG GACCGCTCTG CAAGTGGCTG CCTACCTGGG CCAGGTGGAG 
TTGATACGGC TGCTGCTACA AGCCAGGGCG GGCGTGGACC TGCCGGACGA CGAGGGCAAC 
ACGGCACTGC ACTACGCGGC CCTGGGGAAC CAGCCCGAGG CCACCAGGGT GCTCCTGAGT 
GCTGGGTGCC GGGCGGACGC CATCAACAGC ACCCAGAGCA CAGCACTGCA CGTGGCCGTG 
CAGAGGGGCT TCCTGGAGGT GGTGCGGGCC CTGTGTGAGC GCGGCTGTGA CGTCAACCTG 
CCCGACGCCC ACTCGGACAC GCCCCTGCAC TCCGCCATCT CGGCGGGCAC TGGAGCCAGC 
GGCATTGTCG AGGTCCTCAC GGAGGTGCCA AACATCGATG TTACCGCCAC CAACAGCCAG 
GGTTTCACCC TGCTGCACCA TGCCTCCCTC AAGGGTCACG CGCTAGCTGT GAGAAAGATT 
CTGGCTCGGG CGCGGCAGCT GGTGGACGCC AAGAAGGAGG ACGGCTTCAC GGCGCTGCAT 
CTGGCTGCCC TCAACAACCA CCGCGAGGTG GCCCAGATCC TCATCCGGGA GGGCCGCTGT 
GACGTGAACG TGCGCAACCG GAAGCTGCAG TCCCCGCTGC ATCTCGCCGT GCAACAGGCC 
CACGTGGGGC TGGTGCCGCT ACTGGTGGAC GCTGGGTGCA GTGTCAACGC CGAGGACGAG 
GAGGGGGACA CAGCCCTGCA CGTGGCGCTG CAGCGTCATC AGCTGCTGCC CCTGGTGGCT 
GATGGGGCCG GGGGGGACCC AGGGCCCTTG CAGCTGCTGT CCAGGCTACA GGCCTCGGGC 
CTCCCCGGCA GCGCGGAGCT GACGGTGGGC GCGGCGGTCG CCTGCTTCCT GGCGCTGGAG 
GGCGCCGACG TGAGCTACAC CAACCACCGC GGTCGGAGCC CGCTGGACCT GGCCGCCGAG 
GGTCGCGTGC TCAAGGCCCT TCAGGGCTGC GCCCAGCGCT TCCGGGAGCG GCAGGCGGGC 
GGGGGCGCGG CCCCGGGCCC CAGGCAAACG CTCGGGACCC CCAACACCGT GACGAACCTG 
CACGTGGGCG CCGCGCCGGG GCCCGAGGCC GCTGAGTGCC TGGTGTGCTC CGAGCTGGCG 
CTGCTGGTGC TGTTCTCGCC GTGCCAGCAC CGCACCGTGT GTGAGGAGTG CGCGCGCAGG 
ATGAAGAAGT GCATCAGGTG CCAGGTGGTC GTCAGCAAGA AACTGCGCCC AGACGGCTCT 
GAGGTGGCGA GCGCCGCCCC CGCCCCCGGC CCGCCGCGCC AGCTGGTGGA GGAGCTGCAG 
AGCCGCTACC GGCAGATGGA GGAACGCATC ACCTGCCCCA TCTGCATCGA CAGCCACATC 
CGCCTCGTGT TCCAGTGCGG CCACGGCGCA TGCGCCCCCT GCGGCTCCGC GCTCAGCGCC 
TGCCCCATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( NM_001170686.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001164157.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01762
Clone ID Related Accession (Same CDS sequence)	NM_001170686.1
Accession Version	NM_001170686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL691432.54, DC402226.1, AB076693.1 and BC037542.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the central coding region, compared to variant 1, resulting in an isoform (2) that is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AB076693.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGAGGGGAAG GGAAACCGGG CCGTGTGGTG 
GACATCCGTG GCTGGGATGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC GTGGGCTGAT 
GGTACCACCA ATGTGTACCG TGTGGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCGG GCGGCTTCTA CTACAAGGAC CACCTCCCAA GGCTCGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGA CGGGCACCGT GCATCGTATC ACGGACCGCG GGGACGTGCG CGTGCAGTTC 
AACCACGAGA CGCGCTGGAC CTTCCACCCC GGGGCGCTCA CCAAGCACCA CTCCTTCTGG 
GTGGGCGACG TGGTCCGGGT CATCGGCGAC CTTGACACAG TGAAGCGGCT GCAGGCTGGG 
CATGGCGAGT GGACGGACGA CATGGCCCCT GCCCTGGGCC GCGTCGGGAA GGTGGTGAAA 
GTGTTTGGAG ACGGGAACCT GCGTGTAGCA GTCGCTGGTC AGCGGTGGAC CTTCAGCCCC 
TCCTGCCTGG TGGCCTACCG GCCCGAGGAG GATGCCAACC TGGACGTGGC CGAGCGCGCC 
CGGGAGAACA AAAGCTCACT GAGCGTGGCC CTGGACAAGC TTCGGGCCCA GAAGAGTGAC 
CCAGAGCACC CGGGAAGGCT GGTGGTGGAG GTGGCGCTGG GTAACGCAGC CCGGGCTCTG 
GACCTGCTGC GGAGGCGCCC AGAGCAGGTG GACACCAAGA ACCAAGGCAG GACCGCTCTG 
CAAGTGGCTG CCTACCTGGG CCAGGTGGAG TTGATACGGC TGCTGCTACA AGCCAGGGCG 
GGCGTGGACC TGCCGGACGA CGAGGGCAAC ACGGCACTGC ACTACGCGGC CCTGGGGAAC 
CAGCCCGAGG CCACCAGGGT GCTCCTGAGT GCTGGGTGCC GGGCGGACGC CATCAACAGC 
ACCCAGAGCA CAGCACTGCA CGTGGCCGTG CAGAGGGGCT TCCTGGAGGT GGTGCGGGCC 
CTGTGTGAGC GCGGCTGTGA CGTCAACCTG CCCGACGCCC ACTCGGACAC GCCCCTGCAC 
TCCGCCATCT CGGCGGGCAC TGGAGCCAGC GGCATTGTCG AGGTCCTCAC GGAGGTGCCA 
AACATCGATG TTACCGCCAC CAACAGCCAG GGTTTCACCC TGCTGCACCA TGCCTCCCTC 
AAGGGTCACG CGCTAGCTGT GAGAAAGATT CTGGCTCGGG CGCGGCAGCT GGTGGACGCC 
AAGAAGGAGG ACGGCTTCAC GGCGCTGCAT CTGGCTGCCC TCAACAACCA CCGCGAGGTG 
GCCCAGATCC TCATCCGGGA GGGCCGCTGT GACGTGAACG TGCGCAACCG GAAGCTGCAG 
TCCCCGCTGC ATCTCGCCGT GCAACAGGCC CACGTGGGGC TGGTGCCGCT ACTGGTGGAC 
GCTGGGTGCA GTGTCAACGC CGAGGACGAG GAGGGGGACA CAGCCCTGCA CGTGGCGCTG 
CAGCGTCATC AGCTGCTGCC CCTGGTGGCT GATGGGGCCG GGGGGGACCC AGGGCCCTTG 
CAGCTGCTGT CCAGGCTACA GGCCTCGGGC CTCCCCGGCA GCGCGGAGCT GACGGTGGGC 
GCGGCGGTCG CCTGCTTCCT GGCGCTGGAG GGCGCCGACG TGAGCTACAC CAACCACCGC 
GGTCGGAGCC CGCTGGACCT GGCCGCCGAG GGTCGCGTGC TCAAGGCCCT TCAGGGCTGC 
GCCCAGCGCT TCCGGGAGCG GCAGGCGGGC GGGGGCGCGG CCCCGGGCCC CAGGCAAACG 
CTCGGGACCC CCAACACCGT GACGAACCTG CACGTGGGCG CCGCGCCGGG GCCCGAGGCC 
GCTGAGTGCC TGGTGTGCTC CGAGCTGGCG CTGCTGGTGC TGTTCTCGCC GTGCCAGCAC 
CGCACCGTGT GTGAGGAGTG CGCGCGCAGG ATGAAGAAGT GCATCAGGTG CCAGGTGGTC 
GTCAGCAAGA AACTGCGCCC AGACGGCTCT GAGGTGGCGA GCGCCGCCCC CGCCCCCGGC 
CCGCCGCGCC AGCTGGTGGA GGAGCTGCAG AGCCGCTACC GGCAGATGGA GGAACGCATC 
ACCTGCCCCA TCTGCATCGA CAGCCACATC CGCCTCGTGT TCCAGTGCGG CCACGGCGCA 
TGCGCCCCCT GCGGCTCCGC GCTCAGCGCC TGCCCCATCT GCCGCCAGCC CATCCGCGAC 
CGCATCCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164157.1
CDS	46..3246
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAARPTMDPSAHRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAETGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_001170686.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170686.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGAGGGGAAG GGAAACCGGG CCGTGTGGTG 
GACATCCGTG GCTGGGATGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC GTGGGCTGAT 
GGTACCACCA ATGTGTACCG TGTGGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCGG GCGGCTTCTA CTACAAGGAC CACCTCCCAA GGCTCGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGA CGGGCACCGT GCATCGTATC ACGGACCGCG GGGACGTGCG CGTGCAGTTC 
AACCACGAGA CGCGCTGGAC CTTCCACCCC GGGGCGCTCA CCAAGCACCA CTCCTTCTGG 
GTGGGCGACG TGGTCCGGGT CATCGGCGAC CTTGACACAG TGAAGCGGCT GCAGGCTGGG 
CATGGCGAGT GGACGGACGA CATGGCCCCT GCCCTGGGCC GCGTCGGGAA GGTGGTGAAA 
GTGTTTGGAG ACGGGAACCT GCGTGTAGCA GTCGCTGGTC AGCGGTGGAC CTTCAGCCCC 
TCCTGCCTGG TGGCCTACCG GCCCGAGGAG GATGCCAACC TGGACGTGGC CGAGCGCGCC 
CGGGAGAACA AAAGCTCACT GAGCGTGGCC CTGGACAAGC TTCGGGCCCA GAAGAGTGAC 
CCAGAGCACC CGGGAAGGCT GGTGGTGGAG GTGGCGCTGG GTAACGCAGC CCGGGCTCTG 
GACCTGCTGC GGAGGCGCCC AGAGCAGGTG GACACCAAGA ACCAAGGCAG GACCGCTCTG 
CAAGTGGCTG CCTACCTGGG CCAGGTGGAG TTGATACGGC TGCTGCTACA AGCCAGGGCG 
GGCGTGGACC TGCCGGACGA CGAGGGCAAC ACGGCACTGC ACTACGCGGC CCTGGGGAAC 
CAGCCCGAGG CCACCAGGGT GCTCCTGAGT GCTGGGTGCC GGGCGGACGC CATCAACAGC 
ACCCAGAGCA CAGCACTGCA CGTGGCCGTG CAGAGGGGCT TCCTGGAGGT GGTGCGGGCC 
CTGTGTGAGC GCGGCTGTGA CGTCAACCTG CCCGACGCCC ACTCGGACAC GCCCCTGCAC 
TCCGCCATCT CGGCGGGCAC TGGAGCCAGC GGCATTGTCG AGGTCCTCAC GGAGGTGCCA 
AACATCGATG TTACCGCCAC CAACAGCCAG GGTTTCACCC TGCTGCACCA TGCCTCCCTC 
AAGGGTCACG CGCTAGCTGT GAGAAAGATT CTGGCTCGGG CGCGGCAGCT GGTGGACGCC 
AAGAAGGAGG ACGGCTTCAC GGCGCTGCAT CTGGCTGCCC TCAACAACCA CCGCGAGGTG 
GCCCAGATCC TCATCCGGGA GGGCCGCTGT GACGTGAACG TGCGCAACCG GAAGCTGCAG 
TCCCCGCTGC ATCTCGCCGT GCAACAGGCC CACGTGGGGC TGGTGCCGCT ACTGGTGGAC 
GCTGGGTGCA GTGTCAACGC CGAGGACGAG GAGGGGGACA CAGCCCTGCA CGTGGCGCTG 
CAGCGTCATC AGCTGCTGCC CCTGGTGGCT GATGGGGCCG GGGGGGACCC AGGGCCCTTG 
CAGCTGCTGT CCAGGCTACA GGCCTCGGGC CTCCCCGGCA GCGCGGAGCT GACGGTGGGC 
GCGGCGGTCG CCTGCTTCCT GGCGCTGGAG GGCGCCGACG TGAGCTACAC CAACCACCGC 
GGTCGGAGCC CGCTGGACCT GGCCGCCGAG GGTCGCGTGC TCAAGGCCCT TCAGGGCTGC 
GCCCAGCGCT TCCGGGAGCG GCAGGCGGGC GGGGGCGCGG CCCCGGGCCC CAGGCAAACG 
CTCGGGACCC CCAACACCGT GACGAACCTG CACGTGGGCG CCGCGCCGGG GCCCGAGGCC 
GCTGAGTGCC TGGTGTGCTC CGAGCTGGCG CTGCTGGTGC TGTTCTCGCC GTGCCAGCAC 
CGCACCGTGT GTGAGGAGTG CGCGCGCAGG ATGAAGAAGT GCATCAGGTG CCAGGTGGTC 
GTCAGCAAGA AACTGCGCCC AGACGGCTCT GAGGTGGCGA GCGCCGCCCC CGCCCCCGGC 
CCGCCGCGCC AGCTGGTGGA GGAGCTGCAG AGCCGCTACC GGCAGATGGA GGAACGCATC 
ACCTGCCCCA TCTGCATCGA CAGCCACATC CGCCTCGTGT TCCAGTGCGG CCACGGCGCA 
TGCGCCCCCT GCGGCTCCGC GCTCAGCGCC TGCCCCATCT GCCGCCAGCC CATCCGCGAC 
CGCATCCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( NM_080875.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_543151.2 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 1, mRNA.

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CloneID	OHu01981
Clone ID Related Accession (Same CDS sequence)	NM_080875.2
Accession Version	NM_080875.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3213bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL691432.54, DC402226.1, AB074480.1 and BC037542.1. This sequence is a reference standard in the RefSeqGene project. On Dec 30, 2009 this sequence version replaced NM_080875.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). ##Evidence-Data-START## Transcript exon combination :: AB074480.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGAGGGGAAG GGAAACCGGG CCGTGTGGTG 
GACATCCGTG GCTGGGATGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC GTGGGCTGAT 
GGTACCACCA ATGTGTACCG TGTGGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCGG GCGGCTTCTA CTACAAGGAC CACCTCCCAA GGCTCGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGT TTATCGGACA GACGGGCACC GTGCATCGTA TCACGGACCG CGGGGACGTG 
CGCGTGCAGT TCAACCACGA GACGCGCTGG ACCTTCCACC CCGGGGCGCT CACCAAGCAC 
CACTCCTTCT GGGTGGGCGA CGTGGTCCGG GTCATCGGCG ACCTTGACAC AGTGAAGCGG 
CTGCAGGCTG GGCATGGCGA GTGGACGGAC GACATGGCCC CTGCCCTGGG CCGCGTCGGG 
AAGGTGGTGA AAGTGTTTGG AGACGGGAAC CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG 
ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC CGGCCCGAGG AGGATGCCAA CCTGGACGTG 
GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA CTGAGCGTGG CCCTGGACAA GCTTCGGGCC 
CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG CTGGTGGTGG AGGTGGCGCT GGGTAACGCA 
GCCCGGGCTC TGGACCTGCT GCGGAGGCGC CCAGAGCAGG TGGACACCAA GAACCAAGGC 
AGGACCGCTC TGCAAGTGGC TGCCTACCTG GGCCAGGTGG AGTTGATACG GCTGCTGCTA 
CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC GACGAGGGCA ACACGGCACT GCACTACGCG 
GCCCTGGGGA ACCAGCCCGA GGCCACCAGG GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC 
GCCATCAACA GCACCCAGAG CACAGCACTG CACGTGGCCG TGCAGAGGGG CTTCCTGGAG 
GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT GACGTCAACC TGCCCGACGC CCACTCGGAC 
ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC ACTGGAGCCA GCGGCATTGT CGAGGTCCTC 
ACGGAGGTGC CAAACATCGA TGTTACCGCC ACCAACAGCC AGGGTTTCAC CCTGCTGCAC 
CATGCCTCCC TCAAGGGTCA CGCGCTAGCT GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG 
CTGGTGGACG CCAAGAAGGA GGACGGCTTC ACGGCGCTGC ATCTGGCTGC CCTCAACAAC 
CACCGCGAGG TGGCCCAGAT CCTCATCCGG GAGGGCCGCT GTGACGTGAA CGTGCGCAAC 
CGGAAGCTGC AGTCCCCGCT GCATCTCGCC GTGCAACAGG CCCACGTGGG GCTGGTGCCG 
CTACTGGTGG ACGCTGGGTG CAGTGTCAAC GCCGAGGACG AGGAGGGGGA CACAGCCCTG 
CACGTGGCGC TGCAGCGTCA TCAGCTGCTG CCCCTGGTGG CTGATGGGGC CGGGGGGGAC 
CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG 
CTGACGGTGG GCGCGGCGGT CGCCTGCTTC CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC 
ACCAACCACC GCGGTCGGAG CCCGCTGGAC CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC 
CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC 
CCCAGGCAAA CGCTCGGGAC CCCCAACACC GTGACGAACC TGCACGTGGG CGCCGCGCCG 
GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG 
CCGTGCCAGC ACCGCACCGT GTGTGAGGAG TGCGCGCGCA GGATGAAGAA GTGCATCAGG 
TGCCAGGTGG TCGTCAGCAA GAAACTGCGC CCAGACGGCT CTGAGGTGGC GAGCGCCGCC 
CCCGCCCCCG GCCCGCCGCG CCAGCTGGTG GAGGAGCTGC AGAGCCGCTA CCGGCAGATG 
GAGGAACGCA TCACCTGCCC CATCTGCATC GACAGCCACA TCCGCCTCGT GTTCCAGTGC 
GGCCACGGCG CATGCGCCCC CTGCGGCTCC GCGCTCAGCG CCTGCCCCAT CTGCCGCCAG 
CCCATCCGCG ACCGCATCCA GATCTTCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_543151.2
CDS	46..3258
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAARPTMDPSAHRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	NM_080875.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_080875.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGAGGGGAAG GGAAACCGGG CCGTGTGGTG 
GACATCCGTG GCTGGGATGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC GTGGGCTGAT 
GGTACCACCA ATGTGTACCG TGTGGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCGG GCGGCTTCTA CTACAAGGAC CACCTCCCAA GGCTCGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGT TTATCGGACA GACGGGCACC GTGCATCGTA TCACGGACCG CGGGGACGTG 
CGCGTGCAGT TCAACCACGA GACGCGCTGG ACCTTCCACC CCGGGGCGCT CACCAAGCAC 
CACTCCTTCT GGGTGGGCGA CGTGGTCCGG GTCATCGGCG ACCTTGACAC AGTGAAGCGG 
CTGCAGGCTG GGCATGGCGA GTGGACGGAC GACATGGCCC CTGCCCTGGG CCGCGTCGGG 
AAGGTGGTGA AAGTGTTTGG AGACGGGAAC CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG 
ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC CGGCCCGAGG AGGATGCCAA CCTGGACGTG 
GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA CTGAGCGTGG CCCTGGACAA GCTTCGGGCC 
CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG CTGGTGGTGG AGGTGGCGCT GGGTAACGCA 
GCCCGGGCTC TGGACCTGCT GCGGAGGCGC CCAGAGCAGG TGGACACCAA GAACCAAGGC 
AGGACCGCTC TGCAAGTGGC TGCCTACCTG GGCCAGGTGG AGTTGATACG GCTGCTGCTA 
CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC GACGAGGGCA ACACGGCACT GCACTACGCG 
GCCCTGGGGA ACCAGCCCGA GGCCACCAGG GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC 
GCCATCAACA GCACCCAGAG CACAGCACTG CACGTGGCCG TGCAGAGGGG CTTCCTGGAG 
GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT GACGTCAACC TGCCCGACGC CCACTCGGAC 
ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC ACTGGAGCCA GCGGCATTGT CGAGGTCCTC 
ACGGAGGTGC CAAACATCGA TGTTACCGCC ACCAACAGCC AGGGTTTCAC CCTGCTGCAC 
CATGCCTCCC TCAAGGGTCA CGCGCTAGCT GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG 
CTGGTGGACG CCAAGAAGGA GGACGGCTTC ACGGCGCTGC ATCTGGCTGC CCTCAACAAC 
CACCGCGAGG TGGCCCAGAT CCTCATCCGG GAGGGCCGCT GTGACGTGAA CGTGCGCAAC 
CGGAAGCTGC AGTCCCCGCT GCATCTCGCC GTGCAACAGG CCCACGTGGG GCTGGTGCCG 
CTACTGGTGG ACGCTGGGTG CAGTGTCAAC GCCGAGGACG AGGAGGGGGA CACAGCCCTG 
CACGTGGCGC TGCAGCGTCA TCAGCTGCTG CCCCTGGTGG CTGATGGGGC CGGGGGGGAC 
CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG 
CTGACGGTGG GCGCGGCGGT CGCCTGCTTC CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC 
ACCAACCACC GCGGTCGGAG CCCGCTGGAC CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC 
CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC 
CCCAGGCAAA CGCTCGGGAC CCCCAACACC GTGACGAACC TGCACGTGGG CGCCGCGCCG 
GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG 
CCGTGCCAGC ACCGCACCGT GTGTGAGGAG TGCGCGCGCA GGATGAAGAA GTGCATCAGG 
TGCCAGGTGG TCGTCAGCAA GAAACTGCGC CCAGACGGCT CTGAGGTGGC GAGCGCCGCC 
CCCGCCCCCG GCCCGCCGCG CCAGCTGGTG GAGGAGCTGC AGAGCCGCTA CCGGCAGATG 
GAGGAACGCA TCACCTGCCC CATCTGCATC GACAGCCACA TCCGCCTCGT GTTCCAGTGC 
GGCCACGGCG CATGCGCCCC CTGCGGCTCC GCGCTCAGCG CCTGCCCCAT CTGCCGCCAG 
CCCATCCGCG ACCGCATCCA GATCTTCGTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_006710372.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_006710435.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X22, mRNA.

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CloneID	OHu33040
Clone ID Related Accession (Same CDS sequence)	XM_006710372.1
Accession Version	XM_006710372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGAGGGGAAG GGAAACCGGG CCGTGTGGTG 
GACATCCGTG GCTGGGATGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC GTGGGCTGAT 
GGTACCACCA ATGTGTACCG TGTGGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCGG GCGGCTTCTA CTACAAGGAC CACCTCCCAA GGCTCGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGC ACCACTCCTT CTGGGTGGGC GACGTGGTCC GGGTCATCGG CGACCTTGAC 
ACAGTGAAGC GGCTGCAGGC TGGGCATGGC GAGTGGACGG ACGACATGGC CCCTGCCCTG 
GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT GGAGACGGGA ACCTGCGTGT AGCAGTCGCT 
GGTCAGCGGT GGACCTTCAG CCCCTCCTGC CTGGTGGCCT ACCGGCCCGA GGAGGATGCC 
AACCTGGACG TGGCCGAGCG CGCCCGGGAG AACAAAAGCT CACTGAGCGT GGCCCTGGAC 
AAGCTTCGGG CCCAGAAGAG TGACCCAGAG CACCCGGGAA GGCTGGTGGT GGAGGTGGCG 
CTGGGTAACG CAGCCCGGGC TCTGGACCTG CTGCGGAGGC GCCCAGAGCA GGTGGACACC 
AAGAACCAAG GCAGGACCGC TCTGCAAGTG GCTGCCTACC TGGGCCAGGT GGAGTTGATA 
CGGCTGCTGC TACAAGCCAG GGCGGGCGTG GACCTGCCGG ACGACGAGGG CAACACGGCA 
CTGCACTACG CGGCCCTGGG GAACCAGCCC GAGGCCACCA GGGTGCTCCT GAGTGCTGGG 
TGCCGGGCGG ACGCCATCAA CAGCACCCAG AGCACAGCAC TGCACGTGGC CGTGCAGAGG 
GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT GAGCGCGGCT GTGACGTCAA CCTGCCCGAC 
GCCCACTCGG ACACGCCCCT GCACTCCGCC ATCTCGGCGG GCACTGGAGC CAGCGGCATT 
GTCGAGGTCC TCACGGAGGT GCCAAACATC GATGTTACCG CCACCAACAG CCAGGGTTTC 
ACCCTGCTGC ACCATGCCTC CCTCAAGGGT CACGCGCTAG CTGTGAGAAA GATTCTGGCT 
CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG GAGGACGGCT TCACGGCGCT GCATCTGGCT 
GCCCTCAACA ACCACCGCGA GGTGGCCCAG ATCCTCATCC GGGAGGGCCG CTGTGACGTG 
AACGTGCGCA ACCGGAAGCT GCAGTCCCCG CTGCATCTCG CCGTGCAACA GGCCCACGTG 
GGGCTGGTGC CGCTACTGGT GGACGCTGGG TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG 
GACACAGCCC TGCACGTGGC GCTGCAGCGT CATCAGCTGC TGCCCCTGGT GGCTGATGGG 
GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG CTGTCCAGGC TACAGGCCTC GGGCCTCCCC 
GGCAGCGCGG AGCTGACGGT GGGCGCGGCG GTCGCCTGCT TCCTGGCGCT GGAGGGCGCC 
GACGTGAGCT ACACCAACCA CCGCGGTCGG AGCCCGCTGG ACCTGGCCGC CGAGGGTCGC 
GTGCTCAAGG CCCTTCAGGG CTGCGCCCAG CGCTTCCGGG AGCGGCAGGC GGGCGGGGGC 
GCGGCCCCGG GCCCCAGGCA AACGCTCGGG ACCCCCAACA CCGTGACGAA CCTGCACGTG 
GGCGCCGCGC CGGGGCCCGA GGCCGCTGAG TGCCTGGTGT GCTCCGAGCT GGCGCTGCTG 
GTGCTGTTCT CGCCGTGCCA GCACCGCACC GTGTGTGAGG AGTGCGCGCG CAGGATGAAG 
AAGTGCATCA GGTGCCAGGT GGTCGTCAGC AAGAAACTGC GCCCAGACGG CTCTGAGGTG 
GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC 
TACCGGCAGA TGGAGGAACG CATCACCTGC CCCATCTGCA TCGACAGCCA CATCCGCCTC 
GTGTTCCAGT GCGGCCACGG CGCATGCGCC CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC 
ATCTGCCGCC AGCCCATCCG CGACCGCATC CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006710435.1
CDS	18..3122
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAARPTMDPSAHRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_006710372.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006710372.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGAGGGGAAG GGAAACCGGG CCGTGTGGTG 
GACATCCGTG GCTGGGATGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC GTGGGCTGAT 
GGTACCACCA ATGTGTACCG TGTGGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCGG GCGGCTTCTA CTACAAGGAC CACCTCCCAA GGCTCGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGC ACCACTCCTT CTGGGTGGGC GACGTGGTCC GGGTCATCGG CGACCTTGAC 
ACAGTGAAGC GGCTGCAGGC TGGGCATGGC GAGTGGACGG ACGACATGGC CCCTGCCCTG 
GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT GGAGACGGGA ACCTGCGTGT AGCAGTCGCT 
GGTCAGCGGT GGACCTTCAG CCCCTCCTGC CTGGTGGCCT ACCGGCCCGA GGAGGATGCC 
AACCTGGACG TGGCCGAGCG CGCCCGGGAG AACAAAAGCT CACTGAGCGT GGCCCTGGAC 
AAGCTTCGGG CCCAGAAGAG TGACCCAGAG CACCCGGGAA GGCTGGTGGT GGAGGTGGCG 
CTGGGTAACG CAGCCCGGGC TCTGGACCTG CTGCGGAGGC GCCCAGAGCA GGTGGACACC 
AAGAACCAAG GCAGGACCGC TCTGCAAGTG GCTGCCTACC TGGGCCAGGT GGAGTTGATA 
CGGCTGCTGC TACAAGCCAG GGCGGGCGTG GACCTGCCGG ACGACGAGGG CAACACGGCA 
CTGCACTACG CGGCCCTGGG GAACCAGCCC GAGGCCACCA GGGTGCTCCT GAGTGCTGGG 
TGCCGGGCGG ACGCCATCAA CAGCACCCAG AGCACAGCAC TGCACGTGGC CGTGCAGAGG 
GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT GAGCGCGGCT GTGACGTCAA CCTGCCCGAC 
GCCCACTCGG ACACGCCCCT GCACTCCGCC ATCTCGGCGG GCACTGGAGC CAGCGGCATT 
GTCGAGGTCC TCACGGAGGT GCCAAACATC GATGTTACCG CCACCAACAG CCAGGGTTTC 
ACCCTGCTGC ACCATGCCTC CCTCAAGGGT CACGCGCTAG CTGTGAGAAA GATTCTGGCT 
CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG GAGGACGGCT TCACGGCGCT GCATCTGGCT 
GCCCTCAACA ACCACCGCGA GGTGGCCCAG ATCCTCATCC GGGAGGGCCG CTGTGACGTG 
AACGTGCGCA ACCGGAAGCT GCAGTCCCCG CTGCATCTCG CCGTGCAACA GGCCCACGTG 
GGGCTGGTGC CGCTACTGGT GGACGCTGGG TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG 
GACACAGCCC TGCACGTGGC GCTGCAGCGT CATCAGCTGC TGCCCCTGGT GGCTGATGGG 
GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG CTGTCCAGGC TACAGGCCTC GGGCCTCCCC 
GGCAGCGCGG AGCTGACGGT GGGCGCGGCG GTCGCCTGCT TCCTGGCGCT GGAGGGCGCC 
GACGTGAGCT ACACCAACCA CCGCGGTCGG AGCCCGCTGG ACCTGGCCGC CGAGGGTCGC 
GTGCTCAAGG CCCTTCAGGG CTGCGCCCAG CGCTTCCGGG AGCGGCAGGC GGGCGGGGGC 
GCGGCCCCGG GCCCCAGGCA AACGCTCGGG ACCCCCAACA CCGTGACGAA CCTGCACGTG 
GGCGCCGCGC CGGGGCCCGA GGCCGCTGAG TGCCTGGTGT GCTCCGAGCT GGCGCTGCTG 
GTGCTGTTCT CGCCGTGCCA GCACCGCACC GTGTGTGAGG AGTGCGCGCG CAGGATGAAG 
AAGTGCATCA GGTGCCAGGT GGTCGTCAGC AAGAAACTGC GCCCAGACGG CTCTGAGGTG 
GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC 
TACCGGCAGA TGGAGGAACG CATCACCTGC CCCATCTGCA TCGACAGCCA CATCCGCCTC 
GTGTTCCAGT GCGGCCACGG CGCATGCGCC CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC 
ATCTGCCGCC AGCCCATCCG CGACCGCATC CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_011540731.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539033.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu75979
Clone ID Related Accession (Same CDS sequence)	XM_011540731.2 , XM_011540731.3
Accession Version	XM_011540731.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540731.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGACA 
TGGGGCGCCG GCTGCTGGGG CTGCCAGGTG CCAGGAGACG CCTCCCTCGG GCCTGCCCCG 
GCGCCCGCCC TCACCGGCGT CTGTCCTGCC GCCCAGCTAC AGGCCTCGGG CCTCCCCGGC 
AGCGCGGAGC TGACGGTGGG CGCGGCGGTC GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC 
GTGAGCTACA CCAACCACCG CGGTCGGAGC CCGCTGGACC TGGCCGCCGA GGGTCGCGTG 
CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG 
GCCCCGGGCC CCAGGCAAAC GCTCGGGACC CCCAACACCG TGACGAACCT GCACGTGGGC 
GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG 
CTGTTCTCGC CGTGCCAGCA CCGCACCGTG TGTGAGGAGT GCGCGCGCAG GATGAAGAAG 
TGCATCAGGT GCCAGGTGGT CGTCAGCAAG AAACTGCGCC CAGACGGCTC TGAGGTGGCG 
AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC 
CGGCAGATGG AGGAACGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG 
TTCCAGTGCG GCCACGGCGC ATGCGCCCCC TGCGGCTCCG CGCTCAGCGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539033.1
CDS	273..4094
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_011540731.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540731.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGACA 
TGGGGCGCCG GCTGCTGGGG CTGCCAGGTG CCAGGAGACG CCTCCCTCGG GCCTGCCCCG 
GCGCCCGCCC TCACCGGCGT CTGTCCTGCC GCCCAGCTAC AGGCCTCGGG CCTCCCCGGC 
AGCGCGGAGC TGACGGTGGG CGCGGCGGTC GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC 
GTGAGCTACA CCAACCACCG CGGTCGGAGC CCGCTGGACC TGGCCGCCGA GGGTCGCGTG 
CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG 
GCCCCGGGCC CCAGGCAAAC GCTCGGGACC CCCAACACCG TGACGAACCT GCACGTGGGC 
GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG 
CTGTTCTCGC CGTGCCAGCA CCGCACCGTG TGTGAGGAGT GCGCGCGCAG GATGAAGAAG 
TGCATCAGGT GCCAGGTGGT CGTCAGCAAG AAACTGCGCC CAGACGGCTC TGAGGTGGCG 
AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC 
CGGCAGATGG AGGAACGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG 
TTCCAGTGCG GCCACGGCGC ATGCGCCCCC TGCGGCTCCG CGCTCAGCGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_011540731.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539033.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu75979
Clone ID Related Accession (Same CDS sequence)	XM_011540731.2 , XM_011540731.3
Accession Version	XM_011540731.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011540731.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGACA 
TGGGGCGCCG GCTGCTGGGG CTGCCAGGTG CCAGGAGACG CCTCCCTCGG GCCTGCCCCG 
GCGCCCGCCC TCACCGGCGT CTGTCCTGCC GCCCAGCTAC AGGCCTCGGG CCTCCCCGGC 
AGCGCGGAGC TGACGGTGGG CGCGGCGGTC GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC 
GTGAGCTACA CCAACCACCG CGGTCGGAGC CCGCTGGACC TGGCCGCCGA GGGTCGCGTG 
CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG 
GCCCCGGGCC CCAGGCAAAC GCTCGGGACC CCCAACACCG TGACGAACCT GCACGTGGGC 
GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG 
CTGTTCTCGC CGTGCCAGCA CCGCACCGTG TGTGAGGAGT GCGCGCGCAG GATGAAGAAG 
TGCATCAGGT GCCAGGTGGT CGTCAGCAAG AAACTGCGCC CAGACGGCTC TGAGGTGGCG 
AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC 
CGGCAGATGG AGGAACGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG 
TTCCAGTGCG GCCACGGCGC ATGCGCCCCC TGCGGCTCCG CGCTCAGCGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539033.1
CDS	1715..5536
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_011540731.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540731.3

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGACA 
TGGGGCGCCG GCTGCTGGGG CTGCCAGGTG CCAGGAGACG CCTCCCTCGG GCCTGCCCCG 
GCGCCCGCCC TCACCGGCGT CTGTCCTGCC GCCCAGCTAC AGGCCTCGGG CCTCCCCGGC 
AGCGCGGAGC TGACGGTGGG CGCGGCGGTC GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC 
GTGAGCTACA CCAACCACCG CGGTCGGAGC CCGCTGGACC TGGCCGCCGA GGGTCGCGTG 
CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG 
GCCCCGGGCC CCAGGCAAAC GCTCGGGACC CCCAACACCG TGACGAACCT GCACGTGGGC 
GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG 
CTGTTCTCGC CGTGCCAGCA CCGCACCGTG TGTGAGGAGT GCGCGCGCAG GATGAAGAAG 
TGCATCAGGT GCCAGGTGGT CGTCAGCAAG AAACTGCGCC CAGACGGCTC TGAGGTGGCG 
AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC 
CGGCAGATGG AGGAACGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG 
TTCCAGTGCG GCCACGGCGC ATGCGCCCCC TGCGGCTCCG CGCTCAGCGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_011540736.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539038.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.

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CloneID	OHu75984
Clone ID Related Accession (Same CDS sequence)	XM_011540736.3 , XM_011540736.2
Accession Version	XM_011540736.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011540736.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA 
CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG CTGACGGTGG GCGCGGCGGT CGCCTGCTTC 
CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC ACCAACCACC GCGGTCGGAG CCCGCTGGAC 
CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG 
CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC CCCAGGCAAA CGCTCGGGAC CCCCAACACC 
GTGACGAACC TGCACGTGGG CGCCGCGCCG GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC 
TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG CCGTGCCAGC ACCGCACCGT GTGTGAGGAG 
TGCGCGCGCA GGATGAAGAA GTGCATCAGG TGCCAGGTGG TCGTCAGCAA GAAACTGCGC 
CCAGACGGCT CTGAGGTGGC GAGCGCCGCC CCCGCCCCCG GCCCGCCGCG CCAGCTGGTG 
GAGGAGCTGC AGAGCCGCTA CCGGCAGATG GAGGAACGCA TCACCTGCCC CATCTGCATC 
GACAGCCACA TCCGCCTCGT GTTCCAGTGC GGCCACGGCG CATGCGCCCC CTGCGGCTCC 
GCGCTCAGCG CCTGCCCCAT CTGCCGCCAG CCCATCCGCG ACCGCATCCA GATCTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539038.1
CDS	1699..5421
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_011540736.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540736.3

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA 
CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG CTGACGGTGG GCGCGGCGGT CGCCTGCTTC 
CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC ACCAACCACC GCGGTCGGAG CCCGCTGGAC 
CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG 
CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC CCCAGGCAAA CGCTCGGGAC CCCCAACACC 
GTGACGAACC TGCACGTGGG CGCCGCGCCG GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC 
TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG CCGTGCCAGC ACCGCACCGT GTGTGAGGAG 
TGCGCGCGCA GGATGAAGAA GTGCATCAGG TGCCAGGTGG TCGTCAGCAA GAAACTGCGC 
CCAGACGGCT CTGAGGTGGC GAGCGCCGCC CCCGCCCCCG GCCCGCCGCG CCAGCTGGTG 
GAGGAGCTGC AGAGCCGCTA CCGGCAGATG GAGGAACGCA TCACCTGCCC CATCTGCATC 
GACAGCCACA TCCGCCTCGT GTTCCAGTGC GGCCACGGCG CATGCGCCCC CTGCGGCTCC 
GCGCTCAGCG CCTGCCCCAT CTGCCGCCAG CCCATCCGCG ACCGCATCCA GATCTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_011540736.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539038.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu75984
Clone ID Related Accession (Same CDS sequence)	XM_011540736.3 , XM_011540736.2
Accession Version	XM_011540736.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540736.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA 
CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG CTGACGGTGG GCGCGGCGGT CGCCTGCTTC 
CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC ACCAACCACC GCGGTCGGAG CCCGCTGGAC 
CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG 
CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC CCCAGGCAAA CGCTCGGGAC CCCCAACACC 
GTGACGAACC TGCACGTGGG CGCCGCGCCG GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC 
TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG CCGTGCCAGC ACCGCACCGT GTGTGAGGAG 
TGCGCGCGCA GGATGAAGAA GTGCATCAGG TGCCAGGTGG TCGTCAGCAA GAAACTGCGC 
CCAGACGGCT CTGAGGTGGC GAGCGCCGCC CCCGCCCCCG GCCCGCCGCG CCAGCTGGTG 
GAGGAGCTGC AGAGCCGCTA CCGGCAGATG GAGGAACGCA TCACCTGCCC CATCTGCATC 
GACAGCCACA TCCGCCTCGT GTTCCAGTGC GGCCACGGCG CATGCGCCCC CTGCGGCTCC 
GCGCTCAGCG CCTGCCCCAT CTGCCGCCAG CCCATCCGCG ACCGCATCCA GATCTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539038.1
CDS	273..3995
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_011540736.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540736.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA 
CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG CTGACGGTGG GCGCGGCGGT CGCCTGCTTC 
CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC ACCAACCACC GCGGTCGGAG CCCGCTGGAC 
CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG 
CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC CCCAGGCAAA CGCTCGGGAC CCCCAACACC 
GTGACGAACC TGCACGTGGG CGCCGCGCCG GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC 
TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG CCGTGCCAGC ACCGCACCGT GTGTGAGGAG 
TGCGCGCGCA GGATGAAGAA GTGCATCAGG TGCCAGGTGG TCGTCAGCAA GAAACTGCGC 
CCAGACGGCT CTGAGGTGGC GAGCGCCGCC CCCGCCCCCG GCCCGCCGCG CCAGCTGGTG 
GAGGAGCTGC AGAGCCGCTA CCGGCAGATG GAGGAACGCA TCACCTGCCC CATCTGCATC 
GACAGCCACA TCCGCCTCGT GTTCCAGTGC GGCCACGGCG CATGCGCCCC CTGCGGCTCC 
GCGCTCAGCG CCTGCCCCAT CTGCCGCCAG CCCATCCGCG ACCGCATCCA GATCTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_011540737.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539039.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu75985
Clone ID Related Accession (Same CDS sequence)	XM_011540737.3 , XM_011540737.2
Accession Version	XM_011540737.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011540737.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGCACCAC TCCTTCTGGG TGGGCGACGT GGTCCGGGTC 
ATCGGCGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACGGACGAC 
ATGGCCCCTG CCCTGGGCCG CGTCGGGAAG GTGGTGAAAG TGTTTGGAGA CGGGAACCTG 
CGTGTAGCAG TCGCTGGTCA GCGGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCCGAGGAGG ATGCCAACCT GGACGTGGCC GAGCGCGCCC GGGAGAACAA AAGCTCACTG 
AGCGTGGCCC TGGACAAGCT TCGGGCCCAG AAGAGTGACC CAGAGCACCC GGGAAGGCTG 
GTGGTGGAGG TGGCGCTGGG TAACGCAGCC CGGGCTCTGG ACCTGCTGCG GAGGCGCCCA 
GAGCAGGTGG ACACCAAGAA CCAAGGCAGG ACCGCTCTGC AAGTGGCTGC CTACCTGGGC 
CAGGTGGAGT TGATACGGCT GCTGCTACAA GCCAGGGCGG GCGTGGACCT GCCGGACGAC 
GAGGGCAACA CGGCACTGCA CTACGCGGCC CTGGGGAACC AGCCCGAGGC CACCAGGGTG 
CTCCTGAGTG CTGGGTGCCG GGCGGACGCC ATCAACAGCA CCCAGAGCAC AGCACTGCAC 
GTGGCCGTGC AGAGGGGCTT CCTGGAGGTG GTGCGGGCCC TGTGTGAGCG CGGCTGTGAC 
GTCAACCTGC CCGACGCCCA CTCGGACACG CCCCTGCACT CCGCCATCTC GGCGGGCACT 
GGAGCCAGCG GCATTGTCGA GGTCCTCACG GAGGTGCCAA ACATCGATGT TACCGCCACC 
AACAGCCAGG GTTTCACCCT GCTGCACCAT GCCTCCCTCA AGGGTCACGC GCTAGCTGTG 
AGAAAGATTC TGGCTCGGGC GCGGCAGCTG GTGGACGCCA AGAAGGAGGA CGGCTTCACG 
GCGCTGCATC TGGCTGCCCT CAACAACCAC CGCGAGGTGG CCCAGATCCT CATCCGGGAG 
GGCCGCTGTG ACGTGAACGT GCGCAACCGG AAGCTGCAGT CCCCGCTGCA TCTCGCCGTG 
CAACAGGCCC ACGTGGGGCT GGTGCCGCTA CTGGTGGACG CTGGGTGCAG TGTCAACGCC 
GAGGACGAGG AGGGGGACAC AGCCCTGCAC GTGGCGCTGC AGCGTCATCA GCTGCTGCCC 
CTGGTGGCTG ATGGGGCCGG GGGGGACCCA GGGCCCTTGC AGCTGCTGTC CAGGCTACAG 
GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG 
GCGCTGGAGG GCGCCGACGT GAGCTACACC AACCACCGCG GTCGGAGCCC GCTGGACCTG 
GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG 
CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC AGGCAAACGC TCGGGACCCC CAACACCGTG 
ACGAACCTGC ACGTGGGCGC CGCGCCGGGG CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG TGCCAGCACC GCACCGTGTG TGAGGAGTGC 
GCGCGCAGGA TGAAGAAGTG CATCAGGTGC CAGGTGGTCG TCAGCAAGAA ACTGCGCCCA 
GACGGCTCTG AGGTGGCGAG CGCCGCCCCC GCCCCCGGCC CGCCGCGCCA GCTGGTGGAG 
GAGCTGCAGA GCCGCTACCG GCAGATGGAG GAACGCATCA CCTGCCCCAT CTGCATCGAC 
AGCCACATCC GCCTCGTGTT CCAGTGCGGC CACGGCGCAT GCGCCCCCTG CGGCTCCGCG 
CTCAGCGCCT GCCCCATCTG CCGCCAGCCC ATCCGCGACC GCATCCAGAT CTTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539039.1
CDS	1699..5358
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_011540737.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540737.3

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGCACCAC TCCTTCTGGG TGGGCGACGT GGTCCGGGTC 
ATCGGCGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACGGACGAC 
ATGGCCCCTG CCCTGGGCCG CGTCGGGAAG GTGGTGAAAG TGTTTGGAGA CGGGAACCTG 
CGTGTAGCAG TCGCTGGTCA GCGGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCCGAGGAGG ATGCCAACCT GGACGTGGCC GAGCGCGCCC GGGAGAACAA AAGCTCACTG 
AGCGTGGCCC TGGACAAGCT TCGGGCCCAG AAGAGTGACC CAGAGCACCC GGGAAGGCTG 
GTGGTGGAGG TGGCGCTGGG TAACGCAGCC CGGGCTCTGG ACCTGCTGCG GAGGCGCCCA 
GAGCAGGTGG ACACCAAGAA CCAAGGCAGG ACCGCTCTGC AAGTGGCTGC CTACCTGGGC 
CAGGTGGAGT TGATACGGCT GCTGCTACAA GCCAGGGCGG GCGTGGACCT GCCGGACGAC 
GAGGGCAACA CGGCACTGCA CTACGCGGCC CTGGGGAACC AGCCCGAGGC CACCAGGGTG 
CTCCTGAGTG CTGGGTGCCG GGCGGACGCC ATCAACAGCA CCCAGAGCAC AGCACTGCAC 
GTGGCCGTGC AGAGGGGCTT CCTGGAGGTG GTGCGGGCCC TGTGTGAGCG CGGCTGTGAC 
GTCAACCTGC CCGACGCCCA CTCGGACACG CCCCTGCACT CCGCCATCTC GGCGGGCACT 
GGAGCCAGCG GCATTGTCGA GGTCCTCACG GAGGTGCCAA ACATCGATGT TACCGCCACC 
AACAGCCAGG GTTTCACCCT GCTGCACCAT GCCTCCCTCA AGGGTCACGC GCTAGCTGTG 
AGAAAGATTC TGGCTCGGGC GCGGCAGCTG GTGGACGCCA AGAAGGAGGA CGGCTTCACG 
GCGCTGCATC TGGCTGCCCT CAACAACCAC CGCGAGGTGG CCCAGATCCT CATCCGGGAG 
GGCCGCTGTG ACGTGAACGT GCGCAACCGG AAGCTGCAGT CCCCGCTGCA TCTCGCCGTG 
CAACAGGCCC ACGTGGGGCT GGTGCCGCTA CTGGTGGACG CTGGGTGCAG TGTCAACGCC 
GAGGACGAGG AGGGGGACAC AGCCCTGCAC GTGGCGCTGC AGCGTCATCA GCTGCTGCCC 
CTGGTGGCTG ATGGGGCCGG GGGGGACCCA GGGCCCTTGC AGCTGCTGTC CAGGCTACAG 
GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG 
GCGCTGGAGG GCGCCGACGT GAGCTACACC AACCACCGCG GTCGGAGCCC GCTGGACCTG 
GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG 
CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC AGGCAAACGC TCGGGACCCC CAACACCGTG 
ACGAACCTGC ACGTGGGCGC CGCGCCGGGG CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG TGCCAGCACC GCACCGTGTG TGAGGAGTGC 
GCGCGCAGGA TGAAGAAGTG CATCAGGTGC CAGGTGGTCG TCAGCAAGAA ACTGCGCCCA 
GACGGCTCTG AGGTGGCGAG CGCCGCCCCC GCCCCCGGCC CGCCGCGCCA GCTGGTGGAG 
GAGCTGCAGA GCCGCTACCG GCAGATGGAG GAACGCATCA CCTGCCCCAT CTGCATCGAC 
AGCCACATCC GCCTCGTGTT CCAGTGCGGC CACGGCGCAT GCGCCCCCTG CGGCTCCGCG 
CTCAGCGCCT GCCCCATCTG CCGCCAGCCC ATCCGCGACC GCATCCAGAT CTTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_011540737.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539039.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X12, mRNA.

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CloneID	OHu75985
Clone ID Related Accession (Same CDS sequence)	XM_011540737.3 , XM_011540737.2
Accession Version	XM_011540737.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540737.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGCACCAC TCCTTCTGGG TGGGCGACGT GGTCCGGGTC 
ATCGGCGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACGGACGAC 
ATGGCCCCTG CCCTGGGCCG CGTCGGGAAG GTGGTGAAAG TGTTTGGAGA CGGGAACCTG 
CGTGTAGCAG TCGCTGGTCA GCGGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCCGAGGAGG ATGCCAACCT GGACGTGGCC GAGCGCGCCC GGGAGAACAA AAGCTCACTG 
AGCGTGGCCC TGGACAAGCT TCGGGCCCAG AAGAGTGACC CAGAGCACCC GGGAAGGCTG 
GTGGTGGAGG TGGCGCTGGG TAACGCAGCC CGGGCTCTGG ACCTGCTGCG GAGGCGCCCA 
GAGCAGGTGG ACACCAAGAA CCAAGGCAGG ACCGCTCTGC AAGTGGCTGC CTACCTGGGC 
CAGGTGGAGT TGATACGGCT GCTGCTACAA GCCAGGGCGG GCGTGGACCT GCCGGACGAC 
GAGGGCAACA CGGCACTGCA CTACGCGGCC CTGGGGAACC AGCCCGAGGC CACCAGGGTG 
CTCCTGAGTG CTGGGTGCCG GGCGGACGCC ATCAACAGCA CCCAGAGCAC AGCACTGCAC 
GTGGCCGTGC AGAGGGGCTT CCTGGAGGTG GTGCGGGCCC TGTGTGAGCG CGGCTGTGAC 
GTCAACCTGC CCGACGCCCA CTCGGACACG CCCCTGCACT CCGCCATCTC GGCGGGCACT 
GGAGCCAGCG GCATTGTCGA GGTCCTCACG GAGGTGCCAA ACATCGATGT TACCGCCACC 
AACAGCCAGG GTTTCACCCT GCTGCACCAT GCCTCCCTCA AGGGTCACGC GCTAGCTGTG 
AGAAAGATTC TGGCTCGGGC GCGGCAGCTG GTGGACGCCA AGAAGGAGGA CGGCTTCACG 
GCGCTGCATC TGGCTGCCCT CAACAACCAC CGCGAGGTGG CCCAGATCCT CATCCGGGAG 
GGCCGCTGTG ACGTGAACGT GCGCAACCGG AAGCTGCAGT CCCCGCTGCA TCTCGCCGTG 
CAACAGGCCC ACGTGGGGCT GGTGCCGCTA CTGGTGGACG CTGGGTGCAG TGTCAACGCC 
GAGGACGAGG AGGGGGACAC AGCCCTGCAC GTGGCGCTGC AGCGTCATCA GCTGCTGCCC 
CTGGTGGCTG ATGGGGCCGG GGGGGACCCA GGGCCCTTGC AGCTGCTGTC CAGGCTACAG 
GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG 
GCGCTGGAGG GCGCCGACGT GAGCTACACC AACCACCGCG GTCGGAGCCC GCTGGACCTG 
GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG 
CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC AGGCAAACGC TCGGGACCCC CAACACCGTG 
ACGAACCTGC ACGTGGGCGC CGCGCCGGGG CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG TGCCAGCACC GCACCGTGTG TGAGGAGTGC 
GCGCGCAGGA TGAAGAAGTG CATCAGGTGC CAGGTGGTCG TCAGCAAGAA ACTGCGCCCA 
GACGGCTCTG AGGTGGCGAG CGCCGCCCCC GCCCCCGGCC CGCCGCGCCA GCTGGTGGAG 
GAGCTGCAGA GCCGCTACCG GCAGATGGAG GAACGCATCA CCTGCCCCAT CTGCATCGAC 
AGCCACATCC GCCTCGTGTT CCAGTGCGGC CACGGCGCAT GCGCCCCCTG CGGCTCCGCG 
CTCAGCGCCT GCCCCATCTG CCGCCAGCCC ATCCGCGACC GCATCCAGAT CTTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539039.1
CDS	273..3932
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_011540737.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540737.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGCACCAC TCCTTCTGGG TGGGCGACGT GGTCCGGGTC 
ATCGGCGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACGGACGAC 
ATGGCCCCTG CCCTGGGCCG CGTCGGGAAG GTGGTGAAAG TGTTTGGAGA CGGGAACCTG 
CGTGTAGCAG TCGCTGGTCA GCGGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCCGAGGAGG ATGCCAACCT GGACGTGGCC GAGCGCGCCC GGGAGAACAA AAGCTCACTG 
AGCGTGGCCC TGGACAAGCT TCGGGCCCAG AAGAGTGACC CAGAGCACCC GGGAAGGCTG 
GTGGTGGAGG TGGCGCTGGG TAACGCAGCC CGGGCTCTGG ACCTGCTGCG GAGGCGCCCA 
GAGCAGGTGG ACACCAAGAA CCAAGGCAGG ACCGCTCTGC AAGTGGCTGC CTACCTGGGC 
CAGGTGGAGT TGATACGGCT GCTGCTACAA GCCAGGGCGG GCGTGGACCT GCCGGACGAC 
GAGGGCAACA CGGCACTGCA CTACGCGGCC CTGGGGAACC AGCCCGAGGC CACCAGGGTG 
CTCCTGAGTG CTGGGTGCCG GGCGGACGCC ATCAACAGCA CCCAGAGCAC AGCACTGCAC 
GTGGCCGTGC AGAGGGGCTT CCTGGAGGTG GTGCGGGCCC TGTGTGAGCG CGGCTGTGAC 
GTCAACCTGC CCGACGCCCA CTCGGACACG CCCCTGCACT CCGCCATCTC GGCGGGCACT 
GGAGCCAGCG GCATTGTCGA GGTCCTCACG GAGGTGCCAA ACATCGATGT TACCGCCACC 
AACAGCCAGG GTTTCACCCT GCTGCACCAT GCCTCCCTCA AGGGTCACGC GCTAGCTGTG 
AGAAAGATTC TGGCTCGGGC GCGGCAGCTG GTGGACGCCA AGAAGGAGGA CGGCTTCACG 
GCGCTGCATC TGGCTGCCCT CAACAACCAC CGCGAGGTGG CCCAGATCCT CATCCGGGAG 
GGCCGCTGTG ACGTGAACGT GCGCAACCGG AAGCTGCAGT CCCCGCTGCA TCTCGCCGTG 
CAACAGGCCC ACGTGGGGCT GGTGCCGCTA CTGGTGGACG CTGGGTGCAG TGTCAACGCC 
GAGGACGAGG AGGGGGACAC AGCCCTGCAC GTGGCGCTGC AGCGTCATCA GCTGCTGCCC 
CTGGTGGCTG ATGGGGCCGG GGGGGACCCA GGGCCCTTGC AGCTGCTGTC CAGGCTACAG 
GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG 
GCGCTGGAGG GCGCCGACGT GAGCTACACC AACCACCGCG GTCGGAGCCC GCTGGACCTG 
GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG 
CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC AGGCAAACGC TCGGGACCCC CAACACCGTG 
ACGAACCTGC ACGTGGGCGC CGCGCCGGGG CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG TGCCAGCACC GCACCGTGTG TGAGGAGTGC 
GCGCGCAGGA TGAAGAAGTG CATCAGGTGC CAGGTGGTCG TCAGCAAGAA ACTGCGCCCA 
GACGGCTCTG AGGTGGCGAG CGCCGCCCCC GCCCCCGGCC CGCCGCGCCA GCTGGTGGAG 
GAGCTGCAGA GCCGCTACCG GCAGATGGAG GAACGCATCA CCTGCCCCAT CTGCATCGAC 
AGCCACATCC GCCTCGTGTT CCAGTGCGGC CACGGCGCAT GCGCCCCCTG CGGCTCCGCG 
CTCAGCGCCT GCCCCATCTG CCGCCAGCCC ATCCGCGACC GCATCCAGAT CTTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_011540741.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539043.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X13, mRNA.

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CloneID	OHu75989
Clone ID Related Accession (Same CDS sequence)	XM_011540741.2 , XM_011540741.3
Accession Version	XM_011540741.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540741.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGCCCTG GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT 
GGAGACGGGA ACCTGCGTGT AGCAGTCGCT GGTCAGCGGT GGACCTTCAG CCCCTCCTGC 
CTGGTGGCCT ACCGGCCCGA GGAGGATGCC AACCTGGACG TGGCCGAGCG CGCCCGGGAG 
AACAAAAGCT CACTGAGCGT GGCCCTGGAC AAGCTTCGGG CCCAGAAGAG TGACCCAGAG 
CACCCGGGAA GGCTGGTGGT GGAGGTGGCG CTGGGTAACG CAGCCCGGGC TCTGGACCTG 
CTGCGGAGGC GCCCAGAGCA GGTGGACACC AAGAACCAAG GCAGGACCGC TCTGCAAGTG 
GCTGCCTACC TGGGCCAGGT GGAGTTGATA CGGCTGCTGC TACAAGCCAG GGCGGGCGTG 
GACCTGCCGG ACGACGAGGG CAACACGGCA CTGCACTACG CGGCCCTGGG GAACCAGCCC 
GAGGCCACCA GGGTGCTCCT GAGTGCTGGG TGCCGGGCGG ACGCCATCAA CAGCACCCAG 
AGCACAGCAC TGCACGTGGC CGTGCAGAGG GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT 
GAGCGCGGCT GTGACGTCAA CCTGCCCGAC GCCCACTCGG ACACGCCCCT GCACTCCGCC 
ATCTCGGCGG GCACTGGAGC CAGCGGCATT GTCGAGGTCC TCACGGAGGT GCCAAACATC 
GATGTTACCG CCACCAACAG CCAGGGTTTC ACCCTGCTGC ACCATGCCTC CCTCAAGGGT 
CACGCGCTAG CTGTGAGAAA GATTCTGGCT CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG 
GAGGACGGCT TCACGGCGCT GCATCTGGCT GCCCTCAACA ACCACCGCGA GGTGGCCCAG 
ATCCTCATCC GGGAGGGCCG CTGTGACGTG AACGTGCGCA ACCGGAAGCT GCAGTCCCCG 
CTGCATCTCG CCGTGCAACA GGCCCACGTG GGGCTGGTGC CGCTACTGGT GGACGCTGGG 
TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG GACACAGCCC TGCACGTGGC GCTGCAGCGT 
CATCAGCTGC TGCCCCTGGT GGCTGATGGG GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG 
CTGTCCAGGC TACAGGCCTC GGGCCTCCCC GGCAGCGCGG AGCTGACGGT GGGCGCGGCG 
GTCGCCTGCT TCCTGGCGCT GGAGGGCGCC GACGTGAGCT ACACCAACCA CCGCGGTCGG 
AGCCCGCTGG ACCTGGCCGC CGAGGGTCGC GTGCTCAAGG CCCTTCAGGG CTGCGCCCAG 
CGCTTCCGGG AGCGGCAGGC GGGCGGGGGC GCGGCCCCGG GCCCCAGGCA AACGCTCGGG 
ACCCCCAACA CCGTGACGAA CCTGCACGTG GGCGCCGCGC CGGGGCCCGA GGCCGCTGAG 
TGCCTGGTGT GCTCCGAGCT GGCGCTGCTG GTGCTGTTCT CGCCGTGCCA GCACCGCACC 
GTGTGTGAGG AGTGCGCGCG CAGGATGAAG AAGTGCATCA GGTGCCAGGT GGTCGTCAGC 
AAGAAACTGC GCCCAGACGG CTCTGAGGTG GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG 
CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC TACCGGCAGA TGGAGGAACG CATCACCTGC 
CCCATCTGCA TCGACAGCCA CATCCGCCTC GTGTTCCAGT GCGGCCACGG CGCATGCGCC 
CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC ATCTGCCGCC AGCCCATCCG CGACCGCATC 
CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539043.1
CDS	273..3827
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_011540741.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540741.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGCCCTG GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT 
GGAGACGGGA ACCTGCGTGT AGCAGTCGCT GGTCAGCGGT GGACCTTCAG CCCCTCCTGC 
CTGGTGGCCT ACCGGCCCGA GGAGGATGCC AACCTGGACG TGGCCGAGCG CGCCCGGGAG 
AACAAAAGCT CACTGAGCGT GGCCCTGGAC AAGCTTCGGG CCCAGAAGAG TGACCCAGAG 
CACCCGGGAA GGCTGGTGGT GGAGGTGGCG CTGGGTAACG CAGCCCGGGC TCTGGACCTG 
CTGCGGAGGC GCCCAGAGCA GGTGGACACC AAGAACCAAG GCAGGACCGC TCTGCAAGTG 
GCTGCCTACC TGGGCCAGGT GGAGTTGATA CGGCTGCTGC TACAAGCCAG GGCGGGCGTG 
GACCTGCCGG ACGACGAGGG CAACACGGCA CTGCACTACG CGGCCCTGGG GAACCAGCCC 
GAGGCCACCA GGGTGCTCCT GAGTGCTGGG TGCCGGGCGG ACGCCATCAA CAGCACCCAG 
AGCACAGCAC TGCACGTGGC CGTGCAGAGG GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT 
GAGCGCGGCT GTGACGTCAA CCTGCCCGAC GCCCACTCGG ACACGCCCCT GCACTCCGCC 
ATCTCGGCGG GCACTGGAGC CAGCGGCATT GTCGAGGTCC TCACGGAGGT GCCAAACATC 
GATGTTACCG CCACCAACAG CCAGGGTTTC ACCCTGCTGC ACCATGCCTC CCTCAAGGGT 
CACGCGCTAG CTGTGAGAAA GATTCTGGCT CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG 
GAGGACGGCT TCACGGCGCT GCATCTGGCT GCCCTCAACA ACCACCGCGA GGTGGCCCAG 
ATCCTCATCC GGGAGGGCCG CTGTGACGTG AACGTGCGCA ACCGGAAGCT GCAGTCCCCG 
CTGCATCTCG CCGTGCAACA GGCCCACGTG GGGCTGGTGC CGCTACTGGT GGACGCTGGG 
TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG GACACAGCCC TGCACGTGGC GCTGCAGCGT 
CATCAGCTGC TGCCCCTGGT GGCTGATGGG GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG 
CTGTCCAGGC TACAGGCCTC GGGCCTCCCC GGCAGCGCGG AGCTGACGGT GGGCGCGGCG 
GTCGCCTGCT TCCTGGCGCT GGAGGGCGCC GACGTGAGCT ACACCAACCA CCGCGGTCGG 
AGCCCGCTGG ACCTGGCCGC CGAGGGTCGC GTGCTCAAGG CCCTTCAGGG CTGCGCCCAG 
CGCTTCCGGG AGCGGCAGGC GGGCGGGGGC GCGGCCCCGG GCCCCAGGCA AACGCTCGGG 
ACCCCCAACA CCGTGACGAA CCTGCACGTG GGCGCCGCGC CGGGGCCCGA GGCCGCTGAG 
TGCCTGGTGT GCTCCGAGCT GGCGCTGCTG GTGCTGTTCT CGCCGTGCCA GCACCGCACC 
GTGTGTGAGG AGTGCGCGCG CAGGATGAAG AAGTGCATCA GGTGCCAGGT GGTCGTCAGC 
AAGAAACTGC GCCCAGACGG CTCTGAGGTG GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG 
CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC TACCGGCAGA TGGAGGAACG CATCACCTGC 
CCCATCTGCA TCGACAGCCA CATCCGCCTC GTGTTCCAGT GCGGCCACGG CGCATGCGCC 
CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC ATCTGCCGCC AGCCCATCCG CGACCGCATC 
CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_011540741.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539043.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X13, mRNA.

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CloneID	OHu75989
Clone ID Related Accession (Same CDS sequence)	XM_011540741.2 , XM_011540741.3
Accession Version	XM_011540741.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3555bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011540741.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGCCCTG GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT 
GGAGACGGGA ACCTGCGTGT AGCAGTCGCT GGTCAGCGGT GGACCTTCAG CCCCTCCTGC 
CTGGTGGCCT ACCGGCCCGA GGAGGATGCC AACCTGGACG TGGCCGAGCG CGCCCGGGAG 
AACAAAAGCT CACTGAGCGT GGCCCTGGAC AAGCTTCGGG CCCAGAAGAG TGACCCAGAG 
CACCCGGGAA GGCTGGTGGT GGAGGTGGCG CTGGGTAACG CAGCCCGGGC TCTGGACCTG 
CTGCGGAGGC GCCCAGAGCA GGTGGACACC AAGAACCAAG GCAGGACCGC TCTGCAAGTG 
GCTGCCTACC TGGGCCAGGT GGAGTTGATA CGGCTGCTGC TACAAGCCAG GGCGGGCGTG 
GACCTGCCGG ACGACGAGGG CAACACGGCA CTGCACTACG CGGCCCTGGG GAACCAGCCC 
GAGGCCACCA GGGTGCTCCT GAGTGCTGGG TGCCGGGCGG ACGCCATCAA CAGCACCCAG 
AGCACAGCAC TGCACGTGGC CGTGCAGAGG GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT 
GAGCGCGGCT GTGACGTCAA CCTGCCCGAC GCCCACTCGG ACACGCCCCT GCACTCCGCC 
ATCTCGGCGG GCACTGGAGC CAGCGGCATT GTCGAGGTCC TCACGGAGGT GCCAAACATC 
GATGTTACCG CCACCAACAG CCAGGGTTTC ACCCTGCTGC ACCATGCCTC CCTCAAGGGT 
CACGCGCTAG CTGTGAGAAA GATTCTGGCT CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG 
GAGGACGGCT TCACGGCGCT GCATCTGGCT GCCCTCAACA ACCACCGCGA GGTGGCCCAG 
ATCCTCATCC GGGAGGGCCG CTGTGACGTG AACGTGCGCA ACCGGAAGCT GCAGTCCCCG 
CTGCATCTCG CCGTGCAACA GGCCCACGTG GGGCTGGTGC CGCTACTGGT GGACGCTGGG 
TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG GACACAGCCC TGCACGTGGC GCTGCAGCGT 
CATCAGCTGC TGCCCCTGGT GGCTGATGGG GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG 
CTGTCCAGGC TACAGGCCTC GGGCCTCCCC GGCAGCGCGG AGCTGACGGT GGGCGCGGCG 
GTCGCCTGCT TCCTGGCGCT GGAGGGCGCC GACGTGAGCT ACACCAACCA CCGCGGTCGG 
AGCCCGCTGG ACCTGGCCGC CGAGGGTCGC GTGCTCAAGG CCCTTCAGGG CTGCGCCCAG 
CGCTTCCGGG AGCGGCAGGC GGGCGGGGGC GCGGCCCCGG GCCCCAGGCA AACGCTCGGG 
ACCCCCAACA CCGTGACGAA CCTGCACGTG GGCGCCGCGC CGGGGCCCGA GGCCGCTGAG 
TGCCTGGTGT GCTCCGAGCT GGCGCTGCTG GTGCTGTTCT CGCCGTGCCA GCACCGCACC 
GTGTGTGAGG AGTGCGCGCG CAGGATGAAG AAGTGCATCA GGTGCCAGGT GGTCGTCAGC 
AAGAAACTGC GCCCAGACGG CTCTGAGGTG GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG 
CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC TACCGGCAGA TGGAGGAACG CATCACCTGC 
CCCATCTGCA TCGACAGCCA CATCCGCCTC GTGTTCCAGT GCGGCCACGG CGCATGCGCC 
CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC ATCTGCCGCC AGCCCATCCG CGACCGCATC 
CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539043.1
CDS	1710..5264
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_011540741.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540741.3

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGCCCTG GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT 
GGAGACGGGA ACCTGCGTGT AGCAGTCGCT GGTCAGCGGT GGACCTTCAG CCCCTCCTGC 
CTGGTGGCCT ACCGGCCCGA GGAGGATGCC AACCTGGACG TGGCCGAGCG CGCCCGGGAG 
AACAAAAGCT CACTGAGCGT GGCCCTGGAC AAGCTTCGGG CCCAGAAGAG TGACCCAGAG 
CACCCGGGAA GGCTGGTGGT GGAGGTGGCG CTGGGTAACG CAGCCCGGGC TCTGGACCTG 
CTGCGGAGGC GCCCAGAGCA GGTGGACACC AAGAACCAAG GCAGGACCGC TCTGCAAGTG 
GCTGCCTACC TGGGCCAGGT GGAGTTGATA CGGCTGCTGC TACAAGCCAG GGCGGGCGTG 
GACCTGCCGG ACGACGAGGG CAACACGGCA CTGCACTACG CGGCCCTGGG GAACCAGCCC 
GAGGCCACCA GGGTGCTCCT GAGTGCTGGG TGCCGGGCGG ACGCCATCAA CAGCACCCAG 
AGCACAGCAC TGCACGTGGC CGTGCAGAGG GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT 
GAGCGCGGCT GTGACGTCAA CCTGCCCGAC GCCCACTCGG ACACGCCCCT GCACTCCGCC 
ATCTCGGCGG GCACTGGAGC CAGCGGCATT GTCGAGGTCC TCACGGAGGT GCCAAACATC 
GATGTTACCG CCACCAACAG CCAGGGTTTC ACCCTGCTGC ACCATGCCTC CCTCAAGGGT 
CACGCGCTAG CTGTGAGAAA GATTCTGGCT CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG 
GAGGACGGCT TCACGGCGCT GCATCTGGCT GCCCTCAACA ACCACCGCGA GGTGGCCCAG 
ATCCTCATCC GGGAGGGCCG CTGTGACGTG AACGTGCGCA ACCGGAAGCT GCAGTCCCCG 
CTGCATCTCG CCGTGCAACA GGCCCACGTG GGGCTGGTGC CGCTACTGGT GGACGCTGGG 
TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG GACACAGCCC TGCACGTGGC GCTGCAGCGT 
CATCAGCTGC TGCCCCTGGT GGCTGATGGG GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG 
CTGTCCAGGC TACAGGCCTC GGGCCTCCCC GGCAGCGCGG AGCTGACGGT GGGCGCGGCG 
GTCGCCTGCT TCCTGGCGCT GGAGGGCGCC GACGTGAGCT ACACCAACCA CCGCGGTCGG 
AGCCCGCTGG ACCTGGCCGC CGAGGGTCGC GTGCTCAAGG CCCTTCAGGG CTGCGCCCAG 
CGCTTCCGGG AGCGGCAGGC GGGCGGGGGC GCGGCCCCGG GCCCCAGGCA AACGCTCGGG 
ACCCCCAACA CCGTGACGAA CCTGCACGTG GGCGCCGCGC CGGGGCCCGA GGCCGCTGAG 
TGCCTGGTGT GCTCCGAGCT GGCGCTGCTG GTGCTGTTCT CGCCGTGCCA GCACCGCACC 
GTGTGTGAGG AGTGCGCGCG CAGGATGAAG AAGTGCATCA GGTGCCAGGT GGTCGTCAGC 
AAGAAACTGC GCCCAGACGG CTCTGAGGTG GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG 
CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC TACCGGCAGA TGGAGGAACG CATCACCTGC 
CCCATCTGCA TCGACAGCCA CATCCGCCTC GTGTTCCAGT GCGGCCACGG CGCATGCGCC 
CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC ATCTGCCGCC AGCCCATCCG CGACCGCATC 
CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_011540742.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539044.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X14, mRNA.

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CloneID	OHu75990
Clone ID Related Accession (Same CDS sequence)	XM_011540742.2 , XM_011540742.3
Accession Version	XM_011540742.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011540742.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGCACC ACTCCTTCTG GGTGGGCGAC 
GTGGTCCGGG TCATCGGCGA CCTTGACACA GTGAAGCGGC TGCAGGCTGG GCATGGCGAG 
TGGACGGACG ACATGGCCCC TGCCCTGGGC CGCGTCGGGA AGGTGGTGAA AGTGTTTGGA 
GACGGGAACC TGCGTGTAGC AGTCGCTGGT CAGCGGTGGA CCTTCAGCCC CTCCTGCCTG 
GTGGCCTACC GGCCCGAGGA GGATGCCAAC CTGGACGTGG CCGAGCGCGC CCGGGAGAAC 
AAAAGCTCAC TGAGCGTGGC CCTGGACAAG CTTCGGGCCC AGAAGAGTGA CCCAGAGCAC 
CCGGGAAGGC TGGTGGTGGA GGTGGCGCTG GGTAACGCAG CCCGGGCTCT GGACCTGCTG 
CGGAGGCGCC CAGAGCAGGT GGACACCAAG AACCAAGGCA GGACCGCTCT GCAAGTGGCT 
GCCTACCTGG GCCAGGTGGA GTTGATACGG CTGCTGCTAC AAGCCAGGGC GGGCGTGGAC 
CTGCCGGACG ACGAGGGCAA CACGGCACTG CACTACGCGG CCCTGGGGAA CCAGCCCGAG 
GCCACCAGGG TGCTCCTGAG TGCTGGGTGC CGGGCGGACG CCATCAACAG CACCCAGAGC 
ACAGCACTGC ACGTGGCCGT GCAGAGGGGC TTCCTGGAGG TGGTGCGGGC CCTGTGTGAG 
CGCGGCTGTG ACGTCAACCT GCCCGACGCC CACTCGGACA CGCCCCTGCA CTCCGCCATC 
TCGGCGGGCA CTGGAGCCAG CGGCATTGTC GAGGTCCTCA CGGAGGTGCC AAACATCGAT 
GTTACCGCCA CCAACAGCCA GGGTTTCACC CTGCTGCACC ATGCCTCCCT CAAGGGTCAC 
GCGCTAGCTG TGAGAAAGAT TCTGGCTCGG GCGCGGCAGC TGGTGGACGC CAAGAAGGAG 
GACGGCTTCA CGGCGCTGCA TCTGGCTGCC CTCAACAACC ACCGCGAGGT GGCCCAGATC 
CTCATCCGGG AGGGCCGCTG TGACGTGAAC GTGCGCAACC GGAAGCTGCA GTCCCCGCTG 
CATCTCGCCG TGCAACAGGC CCACGTGGGG CTGGTGCCGC TACTGGTGGA CGCTGGGTGC 
AGTGTCAACG CCGAGGACGA GGAGGGGGAC ACAGCCCTGC ACGTGGCGCT GCAGCGTCAT 
CAGCTGCTGC CCCTGGTGGC TGATGGGGCC GGGGGGGACC CAGGGCCCTT GCAGCTGCTG 
TCCAGGCTAC AGGCCTCGGG CCTCCCCGGC AGCGCGGAGC TGACGGTGGG CGCGGCGGTC 
GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC GTGAGCTACA CCAACCACCG CGGTCGGAGC 
CCGCTGGACC TGGCCGCCGA GGGTCGCGTG CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC 
TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG GCCCCGGGCC CCAGGCAAAC GCTCGGGACC 
CCCAACACCG TGACGAACCT GCACGTGGGC GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC 
CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG CTGTTCTCGC CGTGCCAGCA CCGCACCGTG 
TGTGAGGAGT GCGCGCGCAG GATGAAGAAG TGCATCAGGT GCCAGGTGGT CGTCAGCAAG 
AAACTGCGCC CAGACGGCTC TGAGGTGGCG AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC 
CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAACGCAT CACCTGCCCC 
ATCTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCACGGCGC ATGCGCCCCC 
TGCGGCTCCG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA CCGCATCCAG 
ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539044.1
CDS	273..3824
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_011540742.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540742.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGCACC ACTCCTTCTG GGTGGGCGAC 
GTGGTCCGGG TCATCGGCGA CCTTGACACA GTGAAGCGGC TGCAGGCTGG GCATGGCGAG 
TGGACGGACG ACATGGCCCC TGCCCTGGGC CGCGTCGGGA AGGTGGTGAA AGTGTTTGGA 
GACGGGAACC TGCGTGTAGC AGTCGCTGGT CAGCGGTGGA CCTTCAGCCC CTCCTGCCTG 
GTGGCCTACC GGCCCGAGGA GGATGCCAAC CTGGACGTGG CCGAGCGCGC CCGGGAGAAC 
AAAAGCTCAC TGAGCGTGGC CCTGGACAAG CTTCGGGCCC AGAAGAGTGA CCCAGAGCAC 
CCGGGAAGGC TGGTGGTGGA GGTGGCGCTG GGTAACGCAG CCCGGGCTCT GGACCTGCTG 
CGGAGGCGCC CAGAGCAGGT GGACACCAAG AACCAAGGCA GGACCGCTCT GCAAGTGGCT 
GCCTACCTGG GCCAGGTGGA GTTGATACGG CTGCTGCTAC AAGCCAGGGC GGGCGTGGAC 
CTGCCGGACG ACGAGGGCAA CACGGCACTG CACTACGCGG CCCTGGGGAA CCAGCCCGAG 
GCCACCAGGG TGCTCCTGAG TGCTGGGTGC CGGGCGGACG CCATCAACAG CACCCAGAGC 
ACAGCACTGC ACGTGGCCGT GCAGAGGGGC TTCCTGGAGG TGGTGCGGGC CCTGTGTGAG 
CGCGGCTGTG ACGTCAACCT GCCCGACGCC CACTCGGACA CGCCCCTGCA CTCCGCCATC 
TCGGCGGGCA CTGGAGCCAG CGGCATTGTC GAGGTCCTCA CGGAGGTGCC AAACATCGAT 
GTTACCGCCA CCAACAGCCA GGGTTTCACC CTGCTGCACC ATGCCTCCCT CAAGGGTCAC 
GCGCTAGCTG TGAGAAAGAT TCTGGCTCGG GCGCGGCAGC TGGTGGACGC CAAGAAGGAG 
GACGGCTTCA CGGCGCTGCA TCTGGCTGCC CTCAACAACC ACCGCGAGGT GGCCCAGATC 
CTCATCCGGG AGGGCCGCTG TGACGTGAAC GTGCGCAACC GGAAGCTGCA GTCCCCGCTG 
CATCTCGCCG TGCAACAGGC CCACGTGGGG CTGGTGCCGC TACTGGTGGA CGCTGGGTGC 
AGTGTCAACG CCGAGGACGA GGAGGGGGAC ACAGCCCTGC ACGTGGCGCT GCAGCGTCAT 
CAGCTGCTGC CCCTGGTGGC TGATGGGGCC GGGGGGGACC CAGGGCCCTT GCAGCTGCTG 
TCCAGGCTAC AGGCCTCGGG CCTCCCCGGC AGCGCGGAGC TGACGGTGGG CGCGGCGGTC 
GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC GTGAGCTACA CCAACCACCG CGGTCGGAGC 
CCGCTGGACC TGGCCGCCGA GGGTCGCGTG CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC 
TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG GCCCCGGGCC CCAGGCAAAC GCTCGGGACC 
CCCAACACCG TGACGAACCT GCACGTGGGC GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC 
CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG CTGTTCTCGC CGTGCCAGCA CCGCACCGTG 
TGTGAGGAGT GCGCGCGCAG GATGAAGAAG TGCATCAGGT GCCAGGTGGT CGTCAGCAAG 
AAACTGCGCC CAGACGGCTC TGAGGTGGCG AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC 
CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAACGCAT CACCTGCCCC 
ATCTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCACGGCGC ATGCGCCCCC 
TGCGGCTCCG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA CCGCATCCAG 
ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_011540742.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011539044.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X14, mRNA.

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CloneID	OHu75990
Clone ID Related Accession (Same CDS sequence)	XM_011540742.2 , XM_011540742.3
Accession Version	XM_011540742.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011540742.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGCACC ACTCCTTCTG GGTGGGCGAC 
GTGGTCCGGG TCATCGGCGA CCTTGACACA GTGAAGCGGC TGCAGGCTGG GCATGGCGAG 
TGGACGGACG ACATGGCCCC TGCCCTGGGC CGCGTCGGGA AGGTGGTGAA AGTGTTTGGA 
GACGGGAACC TGCGTGTAGC AGTCGCTGGT CAGCGGTGGA CCTTCAGCCC CTCCTGCCTG 
GTGGCCTACC GGCCCGAGGA GGATGCCAAC CTGGACGTGG CCGAGCGCGC CCGGGAGAAC 
AAAAGCTCAC TGAGCGTGGC CCTGGACAAG CTTCGGGCCC AGAAGAGTGA CCCAGAGCAC 
CCGGGAAGGC TGGTGGTGGA GGTGGCGCTG GGTAACGCAG CCCGGGCTCT GGACCTGCTG 
CGGAGGCGCC CAGAGCAGGT GGACACCAAG AACCAAGGCA GGACCGCTCT GCAAGTGGCT 
GCCTACCTGG GCCAGGTGGA GTTGATACGG CTGCTGCTAC AAGCCAGGGC GGGCGTGGAC 
CTGCCGGACG ACGAGGGCAA CACGGCACTG CACTACGCGG CCCTGGGGAA CCAGCCCGAG 
GCCACCAGGG TGCTCCTGAG TGCTGGGTGC CGGGCGGACG CCATCAACAG CACCCAGAGC 
ACAGCACTGC ACGTGGCCGT GCAGAGGGGC TTCCTGGAGG TGGTGCGGGC CCTGTGTGAG 
CGCGGCTGTG ACGTCAACCT GCCCGACGCC CACTCGGACA CGCCCCTGCA CTCCGCCATC 
TCGGCGGGCA CTGGAGCCAG CGGCATTGTC GAGGTCCTCA CGGAGGTGCC AAACATCGAT 
GTTACCGCCA CCAACAGCCA GGGTTTCACC CTGCTGCACC ATGCCTCCCT CAAGGGTCAC 
GCGCTAGCTG TGAGAAAGAT TCTGGCTCGG GCGCGGCAGC TGGTGGACGC CAAGAAGGAG 
GACGGCTTCA CGGCGCTGCA TCTGGCTGCC CTCAACAACC ACCGCGAGGT GGCCCAGATC 
CTCATCCGGG AGGGCCGCTG TGACGTGAAC GTGCGCAACC GGAAGCTGCA GTCCCCGCTG 
CATCTCGCCG TGCAACAGGC CCACGTGGGG CTGGTGCCGC TACTGGTGGA CGCTGGGTGC 
AGTGTCAACG CCGAGGACGA GGAGGGGGAC ACAGCCCTGC ACGTGGCGCT GCAGCGTCAT 
CAGCTGCTGC CCCTGGTGGC TGATGGGGCC GGGGGGGACC CAGGGCCCTT GCAGCTGCTG 
TCCAGGCTAC AGGCCTCGGG CCTCCCCGGC AGCGCGGAGC TGACGGTGGG CGCGGCGGTC 
GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC GTGAGCTACA CCAACCACCG CGGTCGGAGC 
CCGCTGGACC TGGCCGCCGA GGGTCGCGTG CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC 
TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG GCCCCGGGCC CCAGGCAAAC GCTCGGGACC 
CCCAACACCG TGACGAACCT GCACGTGGGC GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC 
CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG CTGTTCTCGC CGTGCCAGCA CCGCACCGTG 
TGTGAGGAGT GCGCGCGCAG GATGAAGAAG TGCATCAGGT GCCAGGTGGT CGTCAGCAAG 
AAACTGCGCC CAGACGGCTC TGAGGTGGCG AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC 
CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAACGCAT CACCTGCCCC 
ATCTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCACGGCGC ATGCGCCCCC 
TGCGGCTCCG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA CCGCATCCAG 
ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011539044.1
CDS	1699..5250
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_011540742.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011540742.3

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGCACC ACTCCTTCTG GGTGGGCGAC 
GTGGTCCGGG TCATCGGCGA CCTTGACACA GTGAAGCGGC TGCAGGCTGG GCATGGCGAG 
TGGACGGACG ACATGGCCCC TGCCCTGGGC CGCGTCGGGA AGGTGGTGAA AGTGTTTGGA 
GACGGGAACC TGCGTGTAGC AGTCGCTGGT CAGCGGTGGA CCTTCAGCCC CTCCTGCCTG 
GTGGCCTACC GGCCCGAGGA GGATGCCAAC CTGGACGTGG CCGAGCGCGC CCGGGAGAAC 
AAAAGCTCAC TGAGCGTGGC CCTGGACAAG CTTCGGGCCC AGAAGAGTGA CCCAGAGCAC 
CCGGGAAGGC TGGTGGTGGA GGTGGCGCTG GGTAACGCAG CCCGGGCTCT GGACCTGCTG 
CGGAGGCGCC CAGAGCAGGT GGACACCAAG AACCAAGGCA GGACCGCTCT GCAAGTGGCT 
GCCTACCTGG GCCAGGTGGA GTTGATACGG CTGCTGCTAC AAGCCAGGGC GGGCGTGGAC 
CTGCCGGACG ACGAGGGCAA CACGGCACTG CACTACGCGG CCCTGGGGAA CCAGCCCGAG 
GCCACCAGGG TGCTCCTGAG TGCTGGGTGC CGGGCGGACG CCATCAACAG CACCCAGAGC 
ACAGCACTGC ACGTGGCCGT GCAGAGGGGC TTCCTGGAGG TGGTGCGGGC CCTGTGTGAG 
CGCGGCTGTG ACGTCAACCT GCCCGACGCC CACTCGGACA CGCCCCTGCA CTCCGCCATC 
TCGGCGGGCA CTGGAGCCAG CGGCATTGTC GAGGTCCTCA CGGAGGTGCC AAACATCGAT 
GTTACCGCCA CCAACAGCCA GGGTTTCACC CTGCTGCACC ATGCCTCCCT CAAGGGTCAC 
GCGCTAGCTG TGAGAAAGAT TCTGGCTCGG GCGCGGCAGC TGGTGGACGC CAAGAAGGAG 
GACGGCTTCA CGGCGCTGCA TCTGGCTGCC CTCAACAACC ACCGCGAGGT GGCCCAGATC 
CTCATCCGGG AGGGCCGCTG TGACGTGAAC GTGCGCAACC GGAAGCTGCA GTCCCCGCTG 
CATCTCGCCG TGCAACAGGC CCACGTGGGG CTGGTGCCGC TACTGGTGGA CGCTGGGTGC 
AGTGTCAACG CCGAGGACGA GGAGGGGGAC ACAGCCCTGC ACGTGGCGCT GCAGCGTCAT 
CAGCTGCTGC CCCTGGTGGC TGATGGGGCC GGGGGGGACC CAGGGCCCTT GCAGCTGCTG 
TCCAGGCTAC AGGCCTCGGG CCTCCCCGGC AGCGCGGAGC TGACGGTGGG CGCGGCGGTC 
GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC GTGAGCTACA CCAACCACCG CGGTCGGAGC 
CCGCTGGACC TGGCCGCCGA GGGTCGCGTG CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC 
TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG GCCCCGGGCC CCAGGCAAAC GCTCGGGACC 
CCCAACACCG TGACGAACCT GCACGTGGGC GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC 
CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG CTGTTCTCGC CGTGCCAGCA CCGCACCGTG 
TGTGAGGAGT GCGCGCGCAG GATGAAGAAG TGCATCAGGT GCCAGGTGGT CGTCAGCAAG 
AAACTGCGCC CAGACGGCTC TGAGGTGGCG AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC 
CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAACGCAT CACCTGCCCC 
ATCTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCACGGCGC ATGCGCCCCC 
TGCGGCTCCG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA CCGCATCCAG 
ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000349.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855838.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu83158
Clone ID Related Accession (Same CDS sequence)	XM_017000349.2 , XM_017000349.1
Accession Version	XM_017000349.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000349.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGACA 
TGGGGCGCCG GCTGCTGGGG CTGCCAGGTG CCAGGAGACG CCTCCCTCGG GCCTGCCCCG 
GCGCCCGCCC TCACCGGCGT CTGTCCTGCC GCCCAGCTAC AGGCCTCGGG CCTCCCCGGC 
AGCGCGGAGC TGACGGTGGG CGCGGCGGTC GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC 
GTGAGCTACA CCAACCACCG CGGTCGGAGC CCGCTGGACC TGGCCGCCGA GGGTCGCGTG 
CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG 
GCCCCGGGCC CCAGGCAAAC GCTCGGGACC CCCAACACCG TGACGAACCT GCACGTGGGC 
GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG 
CTGTTCTCGC CGTGCCAGCA CCGCACCGTG TGTGAGGGTG AGTGGGGGGC CCCGGGGTGG 
GGAGGCCCGG CTAGTAGGGC CGCAGCCAAC CGCGCTCTCC TCTTCGCAGA GTGCGCGCGC 
AGGATGAAGA AGTGCATCAG GTGCCAGGTG GTCGTCAGCA AGAAACTGCG CCCAGACGGC 
TCTGAGGTGG CGAGCGCCGC CCCCGCCCCC GGCCCGCCGC GCCAGCTGGT GGAGGAGCTG 
CAGAGCCGCT ACCGGCAGAT GGAGGAACGC ATCACCTGCC CCATCTGCAT CGACAGCCAC 
ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCATGCGCCC CCTGCGGCTC CGCGCTCAGC 
GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855838.1
CDS	1725..5618
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000349.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000349.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGACA 
TGGGGCGCCG GCTGCTGGGG CTGCCAGGTG CCAGGAGACG CCTCCCTCGG GCCTGCCCCG 
GCGCCCGCCC TCACCGGCGT CTGTCCTGCC GCCCAGCTAC AGGCCTCGGG CCTCCCCGGC 
AGCGCGGAGC TGACGGTGGG CGCGGCGGTC GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC 
GTGAGCTACA CCAACCACCG CGGTCGGAGC CCGCTGGACC TGGCCGCCGA GGGTCGCGTG 
CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG 
GCCCCGGGCC CCAGGCAAAC GCTCGGGACC CCCAACACCG TGACGAACCT GCACGTGGGC 
GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG 
CTGTTCTCGC CGTGCCAGCA CCGCACCGTG TGTGAGGGTG AGTGGGGGGC CCCGGGGTGG 
GGAGGCCCGG CTAGTAGGGC CGCAGCCAAC CGCGCTCTCC TCTTCGCAGA GTGCGCGCGC 
AGGATGAAGA AGTGCATCAG GTGCCAGGTG GTCGTCAGCA AGAAACTGCG CCCAGACGGC 
TCTGAGGTGG CGAGCGCCGC CCCCGCCCCC GGCCCGCCGC GCCAGCTGGT GGAGGAGCTG 
CAGAGCCGCT ACCGGCAGAT GGAGGAACGC ATCACCTGCC CCATCTGCAT CGACAGCCAC 
ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCATGCGCCC CCTGCGGCTC CGCGCTCAGC 
GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000349.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855838.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

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CloneID	OHu83158
Clone ID Related Accession (Same CDS sequence)	XM_017000349.2 , XM_017000349.1
Accession Version	XM_017000349.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3894bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGACA 
TGGGGCGCCG GCTGCTGGGG CTGCCAGGTG CCAGGAGACG CCTCCCTCGG GCCTGCCCCG 
GCGCCCGCCC TCACCGGCGT CTGTCCTGCC GCCCAGCTAC AGGCCTCGGG CCTCCCCGGC 
AGCGCGGAGC TGACGGTGGG CGCGGCGGTC GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC 
GTGAGCTACA CCAACCACCG CGGTCGGAGC CCGCTGGACC TGGCCGCCGA GGGTCGCGTG 
CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG 
GCCCCGGGCC CCAGGCAAAC GCTCGGGACC CCCAACACCG TGACGAACCT GCACGTGGGC 
GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG 
CTGTTCTCGC CGTGCCAGCA CCGCACCGTG TGTGAGGGTG AGTGGGGGGC CCCGGGGTGG 
GGAGGCCCGG CTAGTAGGGC CGCAGCCAAC CGCGCTCTCC TCTTCGCAGA GTGCGCGCGC 
AGGATGAAGA AGTGCATCAG GTGCCAGGTG GTCGTCAGCA AGAAACTGCG CCCAGACGGC 
TCTGAGGTGG CGAGCGCCGC CCCCGCCCCC GGCCCGCCGC GCCAGCTGGT GGAGGAGCTG 
CAGAGCCGCT ACCGGCAGAT GGAGGAACGC ATCACCTGCC CCATCTGCAT CGACAGCCAC 
ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCATGCGCCC CCTGCGGCTC CGCGCTCAGC 
GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855838.1
CDS	273..4166
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000349.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000349.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGACA 
TGGGGCGCCG GCTGCTGGGG CTGCCAGGTG CCAGGAGACG CCTCCCTCGG GCCTGCCCCG 
GCGCCCGCCC TCACCGGCGT CTGTCCTGCC GCCCAGCTAC AGGCCTCGGG CCTCCCCGGC 
AGCGCGGAGC TGACGGTGGG CGCGGCGGTC GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC 
GTGAGCTACA CCAACCACCG CGGTCGGAGC CCGCTGGACC TGGCCGCCGA GGGTCGCGTG 
CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG 
GCCCCGGGCC CCAGGCAAAC GCTCGGGACC CCCAACACCG TGACGAACCT GCACGTGGGC 
GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG 
CTGTTCTCGC CGTGCCAGCA CCGCACCGTG TGTGAGGGTG AGTGGGGGGC CCCGGGGTGG 
GGAGGCCCGG CTAGTAGGGC CGCAGCCAAC CGCGCTCTCC TCTTCGCAGA GTGCGCGCGC 
AGGATGAAGA AGTGCATCAG GTGCCAGGTG GTCGTCAGCA AGAAACTGCG CCCAGACGGC 
TCTGAGGTGG CGAGCGCCGC CCCCGCCCCC GGCCCGCCGC GCCAGCTGGT GGAGGAGCTG 
CAGAGCCGCT ACCGGCAGAT GGAGGAACGC ATCACCTGCC CCATCTGCAT CGACAGCCAC 
ATCCGCCTCG TGTTCCAGTG CGGCCACGGC GCATGCGCCC CCTGCGGCTC CGCGCTCAGC 
GCCTGCCCCA TCTGCCGCCA GCCCATCCGC GACCGCATCC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000350.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855839.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu83159
Clone ID Related Accession (Same CDS sequence)	XM_017000350.1 , XM_017000350.2
Accession Version	XM_017000350.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGACGG GCACCGTGCA TCGTATCACG 
GACCGCGGGG ACGTGCGCGT GCAGTTCAAC CACGAGACGC GCTGGACCTT CCACCCCGGG 
GCGCTCACCA AGGACACCAG GAAGGCAGGA CAGCTTCGTG GGCGGGAGGG AGGCGGCTGG 
GCTAAGATGC TCCTGCACCA CTCCTTCTGG GTGGGCGACG TGGTCCGGGT CATCGGCGAC 
CTTGACACAG TGAAGCGGCT GCAGGCTGGG CATGGCGAGT GGACGGACGA CATGGCCCCT 
GCCCTGGGCC GCGTCGGGAA GGTGGTGAAA GTGTTTGGAG ACGGGAACCT GCGTGTAGCA 
GTCGCTGGTC AGCGGTGGAC CTTCAGCCCC TCCTGCCTGG TGGCCTACCG GCCCGAGGAG 
GATGCCAACC TGGACGTGGC CGAGCGCGCC CGGGAGAACA AAAGCTCACT GAGCGTGGCC 
CTGGACAAGC TTCGGGCCCA GAAGAGTGAC CCAGAGCACC CGGGAAGGCT GGTGGTGGAG 
GTGGCGCTGG GTAACGCAGC CCGGGCTCTG GACCTGCTGC GGAGGCGCCC AGAGCAGGTG 
GACACCAAGA ACCAAGGCAG GACCGCTCTG CAAGTGGCTG CCTACCTGGG CCAGGTGGAG 
TTGATACGGC TGCTGCTACA AGCCAGGGCG GGCGTGGACC TGCCGGACGA CGAGGGCAAC 
ACGGCACTGC ACTACGCGGC CCTGGGGAAC CAGCCCGAGG CCACCAGGGT GCTCCTGAGT 
GCTGGGTGCC GGGCGGACGC CATCAACAGC ACCCAGAGCA CAGCACTGCA CGTGGCCGTG 
CAGAGGGGCT TCCTGGAGGT GGTGCGGGCC CTGTGTGAGC GCGGCTGTGA CGTCAACCTG 
CCCGACGCCC ACTCGGACAC GCCCCTGCAC TCCGCCATCT CGGCGGGCAC TGGAGCCAGC 
GGCATTGTCG AGGTCCTCAC GGAGGTGCCA AACATCGATG TTACCGCCAC CAACAGCCAG 
GGTTTCACCC TGCTGCACCA TGCCTCCCTC AAGGGTCACG CGCTAGCTGT GAGAAAGATT 
CTGGCTCGGG CGCGGCAGCT GGTGGACGCC AAGAAGGAGG ACGGCTTCAC GGCGCTGCAT 
CTGGCTGCCC TCAACAACCA CCGCGAGGTG GCCCAGATCC TCATCCGGGA GGGCCGCTGT 
GACGTGAACG TGCGCAACCG GAAGCTGCAG TCCCCGCTGC ATCTCGCCGT GCAACAGGCC 
CACGTGGGGC TGGTGCCGCT ACTGGTGGAC GCTGGGTGCA GTGTCAACGC CGAGGACGAG 
GAGGGGGACA CAGCCCTGCA CGTGGCGCTG CAGCGTCATC AGCTGCTGCC CCTGGTGGCT 
GATGGGGCCG GGGGGGACCC AGGGCCCTTG CAGCTGCTGT CCAGGACATG GGGCGCCGGC 
TGCTGGGGCT GCCAGGTGCC AGGAGACGCC TCCCTCGGGC CTGCCCCGGC GCCCGCCCTC 
ACCGGCGTCT GTCCTGCCGC CCAGCTACAG GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG 
ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG GCGCTGGAGG GCGCCGACGT GAGCTACACC 
AACCACCGCG GTCGGAGCCC GCTGGACCTG GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT 
CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC 
AGGCAAACGC TCGGGACCCC CAACACCGTG ACGAACCTGC ACGTGGGCGC CGCGCCGGGG 
CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG 
TGCCAGCACC GCACCGTGTG TGAGGGTGAG TGGGGGGCCC CGGGGTGGGG AGGCCCGGCT 
AGTAGGGCCG CAGCCAACCG CGCTCTCCTC TTCGCAGAGT GCGCGCGCAG GATGAAGAAG 
TGCATCAGGT GCCAGGTGGT CGTCAGCAAG AAACTGCGCC CAGACGGCTC TGAGGTGGCG 
AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC 
CGGCAGATGG AGGAACGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG 
TTCCAGTGCG GCCACGGCGC ATGCGCCCCC TGCGGCTCCG CGCTCAGCGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855839.1
CDS	273..4154
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAETGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000350.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000350.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGACGG GCACCGTGCA TCGTATCACG 
GACCGCGGGG ACGTGCGCGT GCAGTTCAAC CACGAGACGC GCTGGACCTT CCACCCCGGG 
GCGCTCACCA AGGACACCAG GAAGGCAGGA CAGCTTCGTG GGCGGGAGGG AGGCGGCTGG 
GCTAAGATGC TCCTGCACCA CTCCTTCTGG GTGGGCGACG TGGTCCGGGT CATCGGCGAC 
CTTGACACAG TGAAGCGGCT GCAGGCTGGG CATGGCGAGT GGACGGACGA CATGGCCCCT 
GCCCTGGGCC GCGTCGGGAA GGTGGTGAAA GTGTTTGGAG ACGGGAACCT GCGTGTAGCA 
GTCGCTGGTC AGCGGTGGAC CTTCAGCCCC TCCTGCCTGG TGGCCTACCG GCCCGAGGAG 
GATGCCAACC TGGACGTGGC CGAGCGCGCC CGGGAGAACA AAAGCTCACT GAGCGTGGCC 
CTGGACAAGC TTCGGGCCCA GAAGAGTGAC CCAGAGCACC CGGGAAGGCT GGTGGTGGAG 
GTGGCGCTGG GTAACGCAGC CCGGGCTCTG GACCTGCTGC GGAGGCGCCC AGAGCAGGTG 
GACACCAAGA ACCAAGGCAG GACCGCTCTG CAAGTGGCTG CCTACCTGGG CCAGGTGGAG 
TTGATACGGC TGCTGCTACA AGCCAGGGCG GGCGTGGACC TGCCGGACGA CGAGGGCAAC 
ACGGCACTGC ACTACGCGGC CCTGGGGAAC CAGCCCGAGG CCACCAGGGT GCTCCTGAGT 
GCTGGGTGCC GGGCGGACGC CATCAACAGC ACCCAGAGCA CAGCACTGCA CGTGGCCGTG 
CAGAGGGGCT TCCTGGAGGT GGTGCGGGCC CTGTGTGAGC GCGGCTGTGA CGTCAACCTG 
CCCGACGCCC ACTCGGACAC GCCCCTGCAC TCCGCCATCT CGGCGGGCAC TGGAGCCAGC 
GGCATTGTCG AGGTCCTCAC GGAGGTGCCA AACATCGATG TTACCGCCAC CAACAGCCAG 
GGTTTCACCC TGCTGCACCA TGCCTCCCTC AAGGGTCACG CGCTAGCTGT GAGAAAGATT 
CTGGCTCGGG CGCGGCAGCT GGTGGACGCC AAGAAGGAGG ACGGCTTCAC GGCGCTGCAT 
CTGGCTGCCC TCAACAACCA CCGCGAGGTG GCCCAGATCC TCATCCGGGA GGGCCGCTGT 
GACGTGAACG TGCGCAACCG GAAGCTGCAG TCCCCGCTGC ATCTCGCCGT GCAACAGGCC 
CACGTGGGGC TGGTGCCGCT ACTGGTGGAC GCTGGGTGCA GTGTCAACGC CGAGGACGAG 
GAGGGGGACA CAGCCCTGCA CGTGGCGCTG CAGCGTCATC AGCTGCTGCC CCTGGTGGCT 
GATGGGGCCG GGGGGGACCC AGGGCCCTTG CAGCTGCTGT CCAGGACATG GGGCGCCGGC 
TGCTGGGGCT GCCAGGTGCC AGGAGACGCC TCCCTCGGGC CTGCCCCGGC GCCCGCCCTC 
ACCGGCGTCT GTCCTGCCGC CCAGCTACAG GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG 
ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG GCGCTGGAGG GCGCCGACGT GAGCTACACC 
AACCACCGCG GTCGGAGCCC GCTGGACCTG GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT 
CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC 
AGGCAAACGC TCGGGACCCC CAACACCGTG ACGAACCTGC ACGTGGGCGC CGCGCCGGGG 
CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG 
TGCCAGCACC GCACCGTGTG TGAGGGTGAG TGGGGGGCCC CGGGGTGGGG AGGCCCGGCT 
AGTAGGGCCG CAGCCAACCG CGCTCTCCTC TTCGCAGAGT GCGCGCGCAG GATGAAGAAG 
TGCATCAGGT GCCAGGTGGT CGTCAGCAAG AAACTGCGCC CAGACGGCTC TGAGGTGGCG 
AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC 
CGGCAGATGG AGGAACGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG 
TTCCAGTGCG GCCACGGCGC ATGCGCCCCC TGCGGCTCCG CGCTCAGCGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000350.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855839.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu83159
Clone ID Related Accession (Same CDS sequence)	XM_017000350.1 , XM_017000350.2
Accession Version	XM_017000350.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000350.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGACGG GCACCGTGCA TCGTATCACG 
GACCGCGGGG ACGTGCGCGT GCAGTTCAAC CACGAGACGC GCTGGACCTT CCACCCCGGG 
GCGCTCACCA AGGACACCAG GAAGGCAGGA CAGCTTCGTG GGCGGGAGGG AGGCGGCTGG 
GCTAAGATGC TCCTGCACCA CTCCTTCTGG GTGGGCGACG TGGTCCGGGT CATCGGCGAC 
CTTGACACAG TGAAGCGGCT GCAGGCTGGG CATGGCGAGT GGACGGACGA CATGGCCCCT 
GCCCTGGGCC GCGTCGGGAA GGTGGTGAAA GTGTTTGGAG ACGGGAACCT GCGTGTAGCA 
GTCGCTGGTC AGCGGTGGAC CTTCAGCCCC TCCTGCCTGG TGGCCTACCG GCCCGAGGAG 
GATGCCAACC TGGACGTGGC CGAGCGCGCC CGGGAGAACA AAAGCTCACT GAGCGTGGCC 
CTGGACAAGC TTCGGGCCCA GAAGAGTGAC CCAGAGCACC CGGGAAGGCT GGTGGTGGAG 
GTGGCGCTGG GTAACGCAGC CCGGGCTCTG GACCTGCTGC GGAGGCGCCC AGAGCAGGTG 
GACACCAAGA ACCAAGGCAG GACCGCTCTG CAAGTGGCTG CCTACCTGGG CCAGGTGGAG 
TTGATACGGC TGCTGCTACA AGCCAGGGCG GGCGTGGACC TGCCGGACGA CGAGGGCAAC 
ACGGCACTGC ACTACGCGGC CCTGGGGAAC CAGCCCGAGG CCACCAGGGT GCTCCTGAGT 
GCTGGGTGCC GGGCGGACGC CATCAACAGC ACCCAGAGCA CAGCACTGCA CGTGGCCGTG 
CAGAGGGGCT TCCTGGAGGT GGTGCGGGCC CTGTGTGAGC GCGGCTGTGA CGTCAACCTG 
CCCGACGCCC ACTCGGACAC GCCCCTGCAC TCCGCCATCT CGGCGGGCAC TGGAGCCAGC 
GGCATTGTCG AGGTCCTCAC GGAGGTGCCA AACATCGATG TTACCGCCAC CAACAGCCAG 
GGTTTCACCC TGCTGCACCA TGCCTCCCTC AAGGGTCACG CGCTAGCTGT GAGAAAGATT 
CTGGCTCGGG CGCGGCAGCT GGTGGACGCC AAGAAGGAGG ACGGCTTCAC GGCGCTGCAT 
CTGGCTGCCC TCAACAACCA CCGCGAGGTG GCCCAGATCC TCATCCGGGA GGGCCGCTGT 
GACGTGAACG TGCGCAACCG GAAGCTGCAG TCCCCGCTGC ATCTCGCCGT GCAACAGGCC 
CACGTGGGGC TGGTGCCGCT ACTGGTGGAC GCTGGGTGCA GTGTCAACGC CGAGGACGAG 
GAGGGGGACA CAGCCCTGCA CGTGGCGCTG CAGCGTCATC AGCTGCTGCC CCTGGTGGCT 
GATGGGGCCG GGGGGGACCC AGGGCCCTTG CAGCTGCTGT CCAGGACATG GGGCGCCGGC 
TGCTGGGGCT GCCAGGTGCC AGGAGACGCC TCCCTCGGGC CTGCCCCGGC GCCCGCCCTC 
ACCGGCGTCT GTCCTGCCGC CCAGCTACAG GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG 
ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG GCGCTGGAGG GCGCCGACGT GAGCTACACC 
AACCACCGCG GTCGGAGCCC GCTGGACCTG GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT 
CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC 
AGGCAAACGC TCGGGACCCC CAACACCGTG ACGAACCTGC ACGTGGGCGC CGCGCCGGGG 
CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG 
TGCCAGCACC GCACCGTGTG TGAGGGTGAG TGGGGGGCCC CGGGGTGGGG AGGCCCGGCT 
AGTAGGGCCG CAGCCAACCG CGCTCTCCTC TTCGCAGAGT GCGCGCGCAG GATGAAGAAG 
TGCATCAGGT GCCAGGTGGT CGTCAGCAAG AAACTGCGCC CAGACGGCTC TGAGGTGGCG 
AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC 
CGGCAGATGG AGGAACGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG 
TTCCAGTGCG GCCACGGCGC ATGCGCCCCC TGCGGCTCCG CGCTCAGCGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855839.1
CDS	1727..5608
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAETGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000350.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000350.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGACGG GCACCGTGCA TCGTATCACG 
GACCGCGGGG ACGTGCGCGT GCAGTTCAAC CACGAGACGC GCTGGACCTT CCACCCCGGG 
GCGCTCACCA AGGACACCAG GAAGGCAGGA CAGCTTCGTG GGCGGGAGGG AGGCGGCTGG 
GCTAAGATGC TCCTGCACCA CTCCTTCTGG GTGGGCGACG TGGTCCGGGT CATCGGCGAC 
CTTGACACAG TGAAGCGGCT GCAGGCTGGG CATGGCGAGT GGACGGACGA CATGGCCCCT 
GCCCTGGGCC GCGTCGGGAA GGTGGTGAAA GTGTTTGGAG ACGGGAACCT GCGTGTAGCA 
GTCGCTGGTC AGCGGTGGAC CTTCAGCCCC TCCTGCCTGG TGGCCTACCG GCCCGAGGAG 
GATGCCAACC TGGACGTGGC CGAGCGCGCC CGGGAGAACA AAAGCTCACT GAGCGTGGCC 
CTGGACAAGC TTCGGGCCCA GAAGAGTGAC CCAGAGCACC CGGGAAGGCT GGTGGTGGAG 
GTGGCGCTGG GTAACGCAGC CCGGGCTCTG GACCTGCTGC GGAGGCGCCC AGAGCAGGTG 
GACACCAAGA ACCAAGGCAG GACCGCTCTG CAAGTGGCTG CCTACCTGGG CCAGGTGGAG 
TTGATACGGC TGCTGCTACA AGCCAGGGCG GGCGTGGACC TGCCGGACGA CGAGGGCAAC 
ACGGCACTGC ACTACGCGGC CCTGGGGAAC CAGCCCGAGG CCACCAGGGT GCTCCTGAGT 
GCTGGGTGCC GGGCGGACGC CATCAACAGC ACCCAGAGCA CAGCACTGCA CGTGGCCGTG 
CAGAGGGGCT TCCTGGAGGT GGTGCGGGCC CTGTGTGAGC GCGGCTGTGA CGTCAACCTG 
CCCGACGCCC ACTCGGACAC GCCCCTGCAC TCCGCCATCT CGGCGGGCAC TGGAGCCAGC 
GGCATTGTCG AGGTCCTCAC GGAGGTGCCA AACATCGATG TTACCGCCAC CAACAGCCAG 
GGTTTCACCC TGCTGCACCA TGCCTCCCTC AAGGGTCACG CGCTAGCTGT GAGAAAGATT 
CTGGCTCGGG CGCGGCAGCT GGTGGACGCC AAGAAGGAGG ACGGCTTCAC GGCGCTGCAT 
CTGGCTGCCC TCAACAACCA CCGCGAGGTG GCCCAGATCC TCATCCGGGA GGGCCGCTGT 
GACGTGAACG TGCGCAACCG GAAGCTGCAG TCCCCGCTGC ATCTCGCCGT GCAACAGGCC 
CACGTGGGGC TGGTGCCGCT ACTGGTGGAC GCTGGGTGCA GTGTCAACGC CGAGGACGAG 
GAGGGGGACA CAGCCCTGCA CGTGGCGCTG CAGCGTCATC AGCTGCTGCC CCTGGTGGCT 
GATGGGGCCG GGGGGGACCC AGGGCCCTTG CAGCTGCTGT CCAGGACATG GGGCGCCGGC 
TGCTGGGGCT GCCAGGTGCC AGGAGACGCC TCCCTCGGGC CTGCCCCGGC GCCCGCCCTC 
ACCGGCGTCT GTCCTGCCGC CCAGCTACAG GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG 
ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG GCGCTGGAGG GCGCCGACGT GAGCTACACC 
AACCACCGCG GTCGGAGCCC GCTGGACCTG GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT 
CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC 
AGGCAAACGC TCGGGACCCC CAACACCGTG ACGAACCTGC ACGTGGGCGC CGCGCCGGGG 
CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG 
TGCCAGCACC GCACCGTGTG TGAGGGTGAG TGGGGGGCCC CGGGGTGGGG AGGCCCGGCT 
AGTAGGGCCG CAGCCAACCG CGCTCTCCTC TTCGCAGAGT GCGCGCGCAG GATGAAGAAG 
TGCATCAGGT GCCAGGTGGT CGTCAGCAAG AAACTGCGCC CAGACGGCTC TGAGGTGGCG 
AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC 
CGGCAGATGG AGGAACGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG 
TTCCAGTGCG GCCACGGCGC ATGCGCCCCC TGCGGCTCCG CGCTCAGCGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000351.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855840.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.

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CloneID	OHu83160
Clone ID Related Accession (Same CDS sequence)	XM_017000351.2 , XM_017000351.1
Accession Version	XM_017000351.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000351.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGGTTGGA AGCCCAGCGA GGCTAGAGGC CAGTCCCAAA 
GTTTCCAGGC ATCAGGGCTG CAGCCCAGGA GCCTCAAGGC GGCCCGGCGG GCGACTGGAC 
GGCCGGACAG GTCCCGAGCA GCCCGGCCCA CCATGGACCC CTCTGCCCAC AGGTCCCGAG 
CAGCCCCGCC CAACATGGAC CCAGACCCCC AGGCGGGCGT GCAGGTGGGC ATGCGGGTGG 
TGCGCGGCGT GGACTGGAAG TGGGGCCAGC AGGACGGCGG CGAGGGCGGC GTGGGCACGG 
TGGTGGAGCT TGGCCGCCAC GGCAGCCCCT CGACACCCGA CCGCACAGTG GTCGTGCAGT 
GGGACCAGGG CACGCGCACC AACTACCGCG CCGGCTACCA GGGCGCGCAC GACCTGCTGC 
TGCGTCCGGC ACCCCAACAT CATCTGTGAC TGCTGCAAGA AGCACGGGCT GCGGGGGATG 
CGCTGGAAGT GCCGTGTGTG CCTGGACTAC GACCTCTGCA CGCAGTGCTA CATGCACAAC 
AAGCATGAGC TCGCCCACGC CTTCGACCGC TACGAGACCG CTCACTCGCG CCCTGTCACA 
CTGAGTCCCC GCCAGGGCCT CCCGAGGATC CCACTAAGGG GCATCTTCCA GGGAGCGAAG 
GTGGTGCGAG GCCCCGACTG GGAGTGGGGC TCACAGGATG GAGGGGAAGG GAAACCGGGC 
CGTGTGGTGG ACATCCGTGG CTGGGATGTG GAGACAGGCC GGAGTGTGGC CAGCGTGACG 
TGGGCTGATG GTACCACCAA TGTGTACCGT GTGGGCCACA AGGGCAAGGT GGACCTCAAG 
TGTGTGGGCG AGGCAGCGGG CGGCTTCTAC TACAAGGACC ACCTCCCAAG GCTCGGCAAG 
CCGGCGGAGC TGCAGCGCAG GGTGAGTGCT GACAGCCAGC CCTTCCAGCA CGGGGACAAG 
GTCAAGTGTC TGCTGGACAC TGATGTCCTG CGGGAGATGC AGGAAGGCCA CGGCGGCTGG 
AACCCCAGGA TGGCGGAGTT TATCGGACAG ACGGGCACCG TGCATCGTAT CACGGACCGC 
GGGGACGTGC GCGTGCAGTT CAACCACGAG ACGCGCTGGA CCTTCCACCC CGGGGCGCTC 
ACCAAGGACA CCAGGAAGGC AGGACAGCTT CGTGGGCGGG AGGGAGGCGG CTGGGCTAAG 
ATGCTCCTGC ACCACTCCTT CTGGGTGGGC GACGTGGTCC GGGTCATCGG CGACCTTGAC 
ACAGTGAAGC GGCTGCAGGC TGGGCATGGC GAGTGGACGG ACGACATGGC CCCTGCCCTG 
GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT GGAGACGGGA ACCTGCGTGT AGCAGTCGCT 
GGTCAGCGGT GGACCTTCAG CCCCTCCTGC CTGGTGGCCT ACCGGCCCGA GGAGGATGCC 
AACCTGGACG TGGCCGAGCG CGCCCGGGAG AACAAAAGCT CACTGAGCGT GGCCCTGGAC 
AAGCTTCGGG CCCAGAAGAG TGACCCAGAG CACCCGGGAA GGCTGGTGGT GGAGGTGGCG 
CTGGGTAACG CAGCCCGGGC TCTGGACCTG CTGCGGAGGC GCCCAGAGCA GGTGGACACC 
AAGAACCAAG GCAGGACCGC TCTGCAAGTG GCTGCCTACC TGGGCCAGGT GGAGTTGATA 
CGGCTGCTGC TACAAGCCAG GGCGGGCGTG GACCTGCCGG ACGACGAGGG CAACACGGCA 
CTGCACTACG CGGCCCTGGG GAACCAGCCC GAGGCCACCA GGGTGCTCCT GAGTGCTGGG 
TGCCGGGCGG ACGCCATCAA CAGCACCCAG AGCACAGCAC TGCACGTGGC CGTGCAGAGG 
GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT GAGCGCGGCT GTGACGTCAA CCTGCCCGAC 
GCCCACTCGG ACACGCCCCT GCACTCCGCC ATCTCGGCGG GCACTGGAGC CAGCGGCATT 
GTCGAGGTCC TCACGGAGGT GCCAAACATC GATGTTACCG CCACCAACAG CCAGGGTTTC 
ACCCTGCTGC ACCATGCCTC CCTCAAGGGT CACGCGCTAG CTGTGAGAAA GATTCTGGCT 
CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG GAGGACGGCT TCACGGCGCT GCATCTGGCT 
GCCCTCAACA ACCACCGCGA GGTGGCCCAG ATCCTCATCC GGGAGGGCCG CTGTGACGTG 
AACGTGCGCA ACCGGAAGCT GCAGTCCCCG CTGCATCTCG CCGTGCAACA GGCCCACGTG 
GGGCTGGTGC CGCTACTGGT GGACGCTGGG TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG 
GACACAGCCC TGCACGTGGC GCTGCAGCGT CATCAGCTGC TGCCCCTGGT GGCTGATGGG 
GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG CTGTCCAGGA CATGGGGCGC CGGCTGCTGG 
GGCTGCCAGG TGCCAGGAGA CGCCTCCCTC GGGCCTGCCC CGGCGCCCGC CCTCACCGGC 
GTCTGTCCTG CCGCCCAGCT ACAGGCCTCG GGCCTCCCCG GCAGCGCGGA GCTGACGGTG 
GGCGCGGCGG TCGCCTGCTT CCTGGCGCTG GAGGGCGCCG ACGTGAGCTA CACCAACCAC 
CGCGGTCGGA GCCCGCTGGA CCTGGCCGCC GAGGGTCGCG TGCTCAAGGC CCTTCAGGGC 
TGCGCCCAGC GCTTCCGGGA GCGGCAGGCG GGCGGGGGCG CGGCCCCGGG CCCCAGGCAA 
ACGCTCGGGA CCCCCAACAC CGTGACGAAC CTGCACGTGG GCGCCGCGCC GGGGCCCGAG 
GCCGCTGAGT GCCTGGTGTG CTCCGAGCTG GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG 
CACCGCACCG TGTGTGAGGG TGAGTGGGGG GCCCCGGGGT GGGGAGGCCC GGCTAGTAGG 
GCCGCAGCCA ACCGCGCTCT CCTCTTCGCA GAGTGCGCGC GCAGGATGAA GAAGTGCATC 
AGGTGCCAGG TGGTCGTCAG CAAGAAACTG CGCCCAGACG GCTCTGAGGT GGCGAGCGCC 
GCCCCCGCCC CCGGCCCGCC GCGCCAGCTG GTGGAGGAGC TGCAGAGCCG CTACCGGCAG 
ATGGAGGAAC GCATCACCTG CCCCATCTGC ATCGACAGCC ACATCCGCCT CGTGTTCCAG 
TGCGGCCACG GCGCATGCGC CCCCTGCGGC TCCGCGCTCA GCGCCTGCCC CATCTGCCGC 
CAGCCCATCC GCGACCGCAT CCAGATCTTC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855840.1
CDS	1726..5601
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000351.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000351.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGGTTGGA AGCCCAGCGA GGCTAGAGGC CAGTCCCAAA 
GTTTCCAGGC ATCAGGGCTG CAGCCCAGGA GCCTCAAGGC GGCCCGGCGG GCGACTGGAC 
GGCCGGACAG GTCCCGAGCA GCCCGGCCCA CCATGGACCC CTCTGCCCAC AGGTCCCGAG 
CAGCCCCGCC CAACATGGAC CCAGACCCCC AGGCGGGCGT GCAGGTGGGC ATGCGGGTGG 
TGCGCGGCGT GGACTGGAAG TGGGGCCAGC AGGACGGCGG CGAGGGCGGC GTGGGCACGG 
TGGTGGAGCT TGGCCGCCAC GGCAGCCCCT CGACACCCGA CCGCACAGTG GTCGTGCAGT 
GGGACCAGGG CACGCGCACC AACTACCGCG CCGGCTACCA GGGCGCGCAC GACCTGCTGC 
TGCGTCCGGC ACCCCAACAT CATCTGTGAC TGCTGCAAGA AGCACGGGCT GCGGGGGATG 
CGCTGGAAGT GCCGTGTGTG CCTGGACTAC GACCTCTGCA CGCAGTGCTA CATGCACAAC 
AAGCATGAGC TCGCCCACGC CTTCGACCGC TACGAGACCG CTCACTCGCG CCCTGTCACA 
CTGAGTCCCC GCCAGGGCCT CCCGAGGATC CCACTAAGGG GCATCTTCCA GGGAGCGAAG 
GTGGTGCGAG GCCCCGACTG GGAGTGGGGC TCACAGGATG GAGGGGAAGG GAAACCGGGC 
CGTGTGGTGG ACATCCGTGG CTGGGATGTG GAGACAGGCC GGAGTGTGGC CAGCGTGACG 
TGGGCTGATG GTACCACCAA TGTGTACCGT GTGGGCCACA AGGGCAAGGT GGACCTCAAG 
TGTGTGGGCG AGGCAGCGGG CGGCTTCTAC TACAAGGACC ACCTCCCAAG GCTCGGCAAG 
CCGGCGGAGC TGCAGCGCAG GGTGAGTGCT GACAGCCAGC CCTTCCAGCA CGGGGACAAG 
GTCAAGTGTC TGCTGGACAC TGATGTCCTG CGGGAGATGC AGGAAGGCCA CGGCGGCTGG 
AACCCCAGGA TGGCGGAGTT TATCGGACAG ACGGGCACCG TGCATCGTAT CACGGACCGC 
GGGGACGTGC GCGTGCAGTT CAACCACGAG ACGCGCTGGA CCTTCCACCC CGGGGCGCTC 
ACCAAGGACA CCAGGAAGGC AGGACAGCTT CGTGGGCGGG AGGGAGGCGG CTGGGCTAAG 
ATGCTCCTGC ACCACTCCTT CTGGGTGGGC GACGTGGTCC GGGTCATCGG CGACCTTGAC 
ACAGTGAAGC GGCTGCAGGC TGGGCATGGC GAGTGGACGG ACGACATGGC CCCTGCCCTG 
GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT GGAGACGGGA ACCTGCGTGT AGCAGTCGCT 
GGTCAGCGGT GGACCTTCAG CCCCTCCTGC CTGGTGGCCT ACCGGCCCGA GGAGGATGCC 
AACCTGGACG TGGCCGAGCG CGCCCGGGAG AACAAAAGCT CACTGAGCGT GGCCCTGGAC 
AAGCTTCGGG CCCAGAAGAG TGACCCAGAG CACCCGGGAA GGCTGGTGGT GGAGGTGGCG 
CTGGGTAACG CAGCCCGGGC TCTGGACCTG CTGCGGAGGC GCCCAGAGCA GGTGGACACC 
AAGAACCAAG GCAGGACCGC TCTGCAAGTG GCTGCCTACC TGGGCCAGGT GGAGTTGATA 
CGGCTGCTGC TACAAGCCAG GGCGGGCGTG GACCTGCCGG ACGACGAGGG CAACACGGCA 
CTGCACTACG CGGCCCTGGG GAACCAGCCC GAGGCCACCA GGGTGCTCCT GAGTGCTGGG 
TGCCGGGCGG ACGCCATCAA CAGCACCCAG AGCACAGCAC TGCACGTGGC CGTGCAGAGG 
GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT GAGCGCGGCT GTGACGTCAA CCTGCCCGAC 
GCCCACTCGG ACACGCCCCT GCACTCCGCC ATCTCGGCGG GCACTGGAGC CAGCGGCATT 
GTCGAGGTCC TCACGGAGGT GCCAAACATC GATGTTACCG CCACCAACAG CCAGGGTTTC 
ACCCTGCTGC ACCATGCCTC CCTCAAGGGT CACGCGCTAG CTGTGAGAAA GATTCTGGCT 
CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG GAGGACGGCT TCACGGCGCT GCATCTGGCT 
GCCCTCAACA ACCACCGCGA GGTGGCCCAG ATCCTCATCC GGGAGGGCCG CTGTGACGTG 
AACGTGCGCA ACCGGAAGCT GCAGTCCCCG CTGCATCTCG CCGTGCAACA GGCCCACGTG 
GGGCTGGTGC CGCTACTGGT GGACGCTGGG TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG 
GACACAGCCC TGCACGTGGC GCTGCAGCGT CATCAGCTGC TGCCCCTGGT GGCTGATGGG 
GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG CTGTCCAGGA CATGGGGCGC CGGCTGCTGG 
GGCTGCCAGG TGCCAGGAGA CGCCTCCCTC GGGCCTGCCC CGGCGCCCGC CCTCACCGGC 
GTCTGTCCTG CCGCCCAGCT ACAGGCCTCG GGCCTCCCCG GCAGCGCGGA GCTGACGGTG 
GGCGCGGCGG TCGCCTGCTT CCTGGCGCTG GAGGGCGCCG ACGTGAGCTA CACCAACCAC 
CGCGGTCGGA GCCCGCTGGA CCTGGCCGCC GAGGGTCGCG TGCTCAAGGC CCTTCAGGGC 
TGCGCCCAGC GCTTCCGGGA GCGGCAGGCG GGCGGGGGCG CGGCCCCGGG CCCCAGGCAA 
ACGCTCGGGA CCCCCAACAC CGTGACGAAC CTGCACGTGG GCGCCGCGCC GGGGCCCGAG 
GCCGCTGAGT GCCTGGTGTG CTCCGAGCTG GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG 
CACCGCACCG TGTGTGAGGG TGAGTGGGGG GCCCCGGGGT GGGGAGGCCC GGCTAGTAGG 
GCCGCAGCCA ACCGCGCTCT CCTCTTCGCA GAGTGCGCGC GCAGGATGAA GAAGTGCATC 
AGGTGCCAGG TGGTCGTCAG CAAGAAACTG CGCCCAGACG GCTCTGAGGT GGCGAGCGCC 
GCCCCCGCCC CCGGCCCGCC GCGCCAGCTG GTGGAGGAGC TGCAGAGCCG CTACCGGCAG 
ATGGAGGAAC GCATCACCTG CCCCATCTGC ATCGACAGCC ACATCCGCCT CGTGTTCCAG 
TGCGGCCACG GCGCATGCGC CCCCTGCGGC TCCGCGCTCA GCGCCTGCCC CATCTGCCGC 
CAGCCCATCC GCGACCGCAT CCAGATCTTC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000351.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855840.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu83160
Clone ID Related Accession (Same CDS sequence)	XM_017000351.2 , XM_017000351.1
Accession Version	XM_017000351.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGGTTGGA AGCCCAGCGA GGCTAGAGGC CAGTCCCAAA 
GTTTCCAGGC ATCAGGGCTG CAGCCCAGGA GCCTCAAGGC GGCCCGGCGG GCGACTGGAC 
GGCCGGACAG GTCCCGAGCA GCCCGGCCCA CCATGGACCC CTCTGCCCAC AGGTCCCGAG 
CAGCCCCGCC CAACATGGAC CCAGACCCCC AGGCGGGCGT GCAGGTGGGC ATGCGGGTGG 
TGCGCGGCGT GGACTGGAAG TGGGGCCAGC AGGACGGCGG CGAGGGCGGC GTGGGCACGG 
TGGTGGAGCT TGGCCGCCAC GGCAGCCCCT CGACACCCGA CCGCACAGTG GTCGTGCAGT 
GGGACCAGGG CACGCGCACC AACTACCGCG CCGGCTACCA GGGCGCGCAC GACCTGCTGC 
TGCGTCCGGC ACCCCAACAT CATCTGTGAC TGCTGCAAGA AGCACGGGCT GCGGGGGATG 
CGCTGGAAGT GCCGTGTGTG CCTGGACTAC GACCTCTGCA CGCAGTGCTA CATGCACAAC 
AAGCATGAGC TCGCCCACGC CTTCGACCGC TACGAGACCG CTCACTCGCG CCCTGTCACA 
CTGAGTCCCC GCCAGGGCCT CCCGAGGATC CCACTAAGGG GCATCTTCCA GGGAGCGAAG 
GTGGTGCGAG GCCCCGACTG GGAGTGGGGC TCACAGGATG GAGGGGAAGG GAAACCGGGC 
CGTGTGGTGG ACATCCGTGG CTGGGATGTG GAGACAGGCC GGAGTGTGGC CAGCGTGACG 
TGGGCTGATG GTACCACCAA TGTGTACCGT GTGGGCCACA AGGGCAAGGT GGACCTCAAG 
TGTGTGGGCG AGGCAGCGGG CGGCTTCTAC TACAAGGACC ACCTCCCAAG GCTCGGCAAG 
CCGGCGGAGC TGCAGCGCAG GGTGAGTGCT GACAGCCAGC CCTTCCAGCA CGGGGACAAG 
GTCAAGTGTC TGCTGGACAC TGATGTCCTG CGGGAGATGC AGGAAGGCCA CGGCGGCTGG 
AACCCCAGGA TGGCGGAGTT TATCGGACAG ACGGGCACCG TGCATCGTAT CACGGACCGC 
GGGGACGTGC GCGTGCAGTT CAACCACGAG ACGCGCTGGA CCTTCCACCC CGGGGCGCTC 
ACCAAGGACA CCAGGAAGGC AGGACAGCTT CGTGGGCGGG AGGGAGGCGG CTGGGCTAAG 
ATGCTCCTGC ACCACTCCTT CTGGGTGGGC GACGTGGTCC GGGTCATCGG CGACCTTGAC 
ACAGTGAAGC GGCTGCAGGC TGGGCATGGC GAGTGGACGG ACGACATGGC CCCTGCCCTG 
GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT GGAGACGGGA ACCTGCGTGT AGCAGTCGCT 
GGTCAGCGGT GGACCTTCAG CCCCTCCTGC CTGGTGGCCT ACCGGCCCGA GGAGGATGCC 
AACCTGGACG TGGCCGAGCG CGCCCGGGAG AACAAAAGCT CACTGAGCGT GGCCCTGGAC 
AAGCTTCGGG CCCAGAAGAG TGACCCAGAG CACCCGGGAA GGCTGGTGGT GGAGGTGGCG 
CTGGGTAACG CAGCCCGGGC TCTGGACCTG CTGCGGAGGC GCCCAGAGCA GGTGGACACC 
AAGAACCAAG GCAGGACCGC TCTGCAAGTG GCTGCCTACC TGGGCCAGGT GGAGTTGATA 
CGGCTGCTGC TACAAGCCAG GGCGGGCGTG GACCTGCCGG ACGACGAGGG CAACACGGCA 
CTGCACTACG CGGCCCTGGG GAACCAGCCC GAGGCCACCA GGGTGCTCCT GAGTGCTGGG 
TGCCGGGCGG ACGCCATCAA CAGCACCCAG AGCACAGCAC TGCACGTGGC CGTGCAGAGG 
GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT GAGCGCGGCT GTGACGTCAA CCTGCCCGAC 
GCCCACTCGG ACACGCCCCT GCACTCCGCC ATCTCGGCGG GCACTGGAGC CAGCGGCATT 
GTCGAGGTCC TCACGGAGGT GCCAAACATC GATGTTACCG CCACCAACAG CCAGGGTTTC 
ACCCTGCTGC ACCATGCCTC CCTCAAGGGT CACGCGCTAG CTGTGAGAAA GATTCTGGCT 
CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG GAGGACGGCT TCACGGCGCT GCATCTGGCT 
GCCCTCAACA ACCACCGCGA GGTGGCCCAG ATCCTCATCC GGGAGGGCCG CTGTGACGTG 
AACGTGCGCA ACCGGAAGCT GCAGTCCCCG CTGCATCTCG CCGTGCAACA GGCCCACGTG 
GGGCTGGTGC CGCTACTGGT GGACGCTGGG TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG 
GACACAGCCC TGCACGTGGC GCTGCAGCGT CATCAGCTGC TGCCCCTGGT GGCTGATGGG 
GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG CTGTCCAGGA CATGGGGCGC CGGCTGCTGG 
GGCTGCCAGG TGCCAGGAGA CGCCTCCCTC GGGCCTGCCC CGGCGCCCGC CCTCACCGGC 
GTCTGTCCTG CCGCCCAGCT ACAGGCCTCG GGCCTCCCCG GCAGCGCGGA GCTGACGGTG 
GGCGCGGCGG TCGCCTGCTT CCTGGCGCTG GAGGGCGCCG ACGTGAGCTA CACCAACCAC 
CGCGGTCGGA GCCCGCTGGA CCTGGCCGCC GAGGGTCGCG TGCTCAAGGC CCTTCAGGGC 
TGCGCCCAGC GCTTCCGGGA GCGGCAGGCG GGCGGGGGCG CGGCCCCGGG CCCCAGGCAA 
ACGCTCGGGA CCCCCAACAC CGTGACGAAC CTGCACGTGG GCGCCGCGCC GGGGCCCGAG 
GCCGCTGAGT GCCTGGTGTG CTCCGAGCTG GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG 
CACCGCACCG TGTGTGAGGG TGAGTGGGGG GCCCCGGGGT GGGGAGGCCC GGCTAGTAGG 
GCCGCAGCCA ACCGCGCTCT CCTCTTCGCA GAGTGCGCGC GCAGGATGAA GAAGTGCATC 
AGGTGCCAGG TGGTCGTCAG CAAGAAACTG CGCCCAGACG GCTCTGAGGT GGCGAGCGCC 
GCCCCCGCCC CCGGCCCGCC GCGCCAGCTG GTGGAGGAGC TGCAGAGCCG CTACCGGCAG 
ATGGAGGAAC GCATCACCTG CCCCATCTGC ATCGACAGCC ACATCCGCCT CGTGTTCCAG 
TGCGGCCACG GCGCATGCGC CCCCTGCGGC TCCGCGCTCA GCGCCTGCCC CATCTGCCGC 
CAGCCCATCC GCGACCGCAT CCAGATCTTC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855840.1
CDS	273..4148
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000351.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000351.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGGTTGGA AGCCCAGCGA GGCTAGAGGC CAGTCCCAAA 
GTTTCCAGGC ATCAGGGCTG CAGCCCAGGA GCCTCAAGGC GGCCCGGCGG GCGACTGGAC 
GGCCGGACAG GTCCCGAGCA GCCCGGCCCA CCATGGACCC CTCTGCCCAC AGGTCCCGAG 
CAGCCCCGCC CAACATGGAC CCAGACCCCC AGGCGGGCGT GCAGGTGGGC ATGCGGGTGG 
TGCGCGGCGT GGACTGGAAG TGGGGCCAGC AGGACGGCGG CGAGGGCGGC GTGGGCACGG 
TGGTGGAGCT TGGCCGCCAC GGCAGCCCCT CGACACCCGA CCGCACAGTG GTCGTGCAGT 
GGGACCAGGG CACGCGCACC AACTACCGCG CCGGCTACCA GGGCGCGCAC GACCTGCTGC 
TGCGTCCGGC ACCCCAACAT CATCTGTGAC TGCTGCAAGA AGCACGGGCT GCGGGGGATG 
CGCTGGAAGT GCCGTGTGTG CCTGGACTAC GACCTCTGCA CGCAGTGCTA CATGCACAAC 
AAGCATGAGC TCGCCCACGC CTTCGACCGC TACGAGACCG CTCACTCGCG CCCTGTCACA 
CTGAGTCCCC GCCAGGGCCT CCCGAGGATC CCACTAAGGG GCATCTTCCA GGGAGCGAAG 
GTGGTGCGAG GCCCCGACTG GGAGTGGGGC TCACAGGATG GAGGGGAAGG GAAACCGGGC 
CGTGTGGTGG ACATCCGTGG CTGGGATGTG GAGACAGGCC GGAGTGTGGC CAGCGTGACG 
TGGGCTGATG GTACCACCAA TGTGTACCGT GTGGGCCACA AGGGCAAGGT GGACCTCAAG 
TGTGTGGGCG AGGCAGCGGG CGGCTTCTAC TACAAGGACC ACCTCCCAAG GCTCGGCAAG 
CCGGCGGAGC TGCAGCGCAG GGTGAGTGCT GACAGCCAGC CCTTCCAGCA CGGGGACAAG 
GTCAAGTGTC TGCTGGACAC TGATGTCCTG CGGGAGATGC AGGAAGGCCA CGGCGGCTGG 
AACCCCAGGA TGGCGGAGTT TATCGGACAG ACGGGCACCG TGCATCGTAT CACGGACCGC 
GGGGACGTGC GCGTGCAGTT CAACCACGAG ACGCGCTGGA CCTTCCACCC CGGGGCGCTC 
ACCAAGGACA CCAGGAAGGC AGGACAGCTT CGTGGGCGGG AGGGAGGCGG CTGGGCTAAG 
ATGCTCCTGC ACCACTCCTT CTGGGTGGGC GACGTGGTCC GGGTCATCGG CGACCTTGAC 
ACAGTGAAGC GGCTGCAGGC TGGGCATGGC GAGTGGACGG ACGACATGGC CCCTGCCCTG 
GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT GGAGACGGGA ACCTGCGTGT AGCAGTCGCT 
GGTCAGCGGT GGACCTTCAG CCCCTCCTGC CTGGTGGCCT ACCGGCCCGA GGAGGATGCC 
AACCTGGACG TGGCCGAGCG CGCCCGGGAG AACAAAAGCT CACTGAGCGT GGCCCTGGAC 
AAGCTTCGGG CCCAGAAGAG TGACCCAGAG CACCCGGGAA GGCTGGTGGT GGAGGTGGCG 
CTGGGTAACG CAGCCCGGGC TCTGGACCTG CTGCGGAGGC GCCCAGAGCA GGTGGACACC 
AAGAACCAAG GCAGGACCGC TCTGCAAGTG GCTGCCTACC TGGGCCAGGT GGAGTTGATA 
CGGCTGCTGC TACAAGCCAG GGCGGGCGTG GACCTGCCGG ACGACGAGGG CAACACGGCA 
CTGCACTACG CGGCCCTGGG GAACCAGCCC GAGGCCACCA GGGTGCTCCT GAGTGCTGGG 
TGCCGGGCGG ACGCCATCAA CAGCACCCAG AGCACAGCAC TGCACGTGGC CGTGCAGAGG 
GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT GAGCGCGGCT GTGACGTCAA CCTGCCCGAC 
GCCCACTCGG ACACGCCCCT GCACTCCGCC ATCTCGGCGG GCACTGGAGC CAGCGGCATT 
GTCGAGGTCC TCACGGAGGT GCCAAACATC GATGTTACCG CCACCAACAG CCAGGGTTTC 
ACCCTGCTGC ACCATGCCTC CCTCAAGGGT CACGCGCTAG CTGTGAGAAA GATTCTGGCT 
CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG GAGGACGGCT TCACGGCGCT GCATCTGGCT 
GCCCTCAACA ACCACCGCGA GGTGGCCCAG ATCCTCATCC GGGAGGGCCG CTGTGACGTG 
AACGTGCGCA ACCGGAAGCT GCAGTCCCCG CTGCATCTCG CCGTGCAACA GGCCCACGTG 
GGGCTGGTGC CGCTACTGGT GGACGCTGGG TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG 
GACACAGCCC TGCACGTGGC GCTGCAGCGT CATCAGCTGC TGCCCCTGGT GGCTGATGGG 
GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG CTGTCCAGGA CATGGGGCGC CGGCTGCTGG 
GGCTGCCAGG TGCCAGGAGA CGCCTCCCTC GGGCCTGCCC CGGCGCCCGC CCTCACCGGC 
GTCTGTCCTG CCGCCCAGCT ACAGGCCTCG GGCCTCCCCG GCAGCGCGGA GCTGACGGTG 
GGCGCGGCGG TCGCCTGCTT CCTGGCGCTG GAGGGCGCCG ACGTGAGCTA CACCAACCAC 
CGCGGTCGGA GCCCGCTGGA CCTGGCCGCC GAGGGTCGCG TGCTCAAGGC CCTTCAGGGC 
TGCGCCCAGC GCTTCCGGGA GCGGCAGGCG GGCGGGGGCG CGGCCCCGGG CCCCAGGCAA 
ACGCTCGGGA CCCCCAACAC CGTGACGAAC CTGCACGTGG GCGCCGCGCC GGGGCCCGAG 
GCCGCTGAGT GCCTGGTGTG CTCCGAGCTG GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG 
CACCGCACCG TGTGTGAGGG TGAGTGGGGG GCCCCGGGGT GGGGAGGCCC GGCTAGTAGG 
GCCGCAGCCA ACCGCGCTCT CCTCTTCGCA GAGTGCGCGC GCAGGATGAA GAAGTGCATC 
AGGTGCCAGG TGGTCGTCAG CAAGAAACTG CGCCCAGACG GCTCTGAGGT GGCGAGCGCC 
GCCCCCGCCC CCGGCCCGCC GCGCCAGCTG GTGGAGGAGC TGCAGAGCCG CTACCGGCAG 
ATGGAGGAAC GCATCACCTG CCCCATCTGC ATCGACAGCC ACATCCGCCT CGTGTTCCAG 
TGCGGCCACG GCGCATGCGC CCCCTGCGGC TCCGCGCTCA GCGCCTGCCC CATCTGCCGC 
CAGCCCATCC GCGACCGCAT CCAGATCTTC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000352.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855841.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu83161
Clone ID Related Accession (Same CDS sequence)	XM_017000352.1 , XM_017000352.2
Accession Version	XM_017000352.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCCGCCCAAC ATGGACCCAG 
ACCCCCAGGC GGGCGTGCAG GTGGGCATGC GGGTGGTGCG CGGCGTGGAC TGGAAGTGGG 
GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTTGGC CGCCACGGCA 
GCCCCTCGAC ACCCGACCGC ACAGTGGTCG TGCAGTGGGA CCAGGGCACG CGCACCAACT 
ACCGCGCCGG CTACCAGGGC GCGCACGACC TGCTGCTGCG TCCGGCACCC CAACATCATC 
TGTGACTGCT GCAAGAAGCA CGGGCTGCGG GGGATGCGCT GGAAGTGCCG TGTGTGCCTG 
GACTACGACC TCTGCACGCA GTGCTACATG CACAACAAGC ATGAGCTCGC CCACGCCTTC 
GACCGCTACG AGACCGCTCA CTCGCGCCCT GTCACACTGA GTCCCCGCCA GGGCCTCCCG 
AGGATCCCAC TAAGGGGCAT CTTCCAGGGA GCGAAGGTGG TGCGAGGCCC CGACTGGGAG 
TGGGGCTCAC AGGATGGAGG GGAAGGGAAA CCGGGCCGTG TGGTGGACAT CCGTGGCTGG 
GATGTGGAGA CAGGCCGGAG TGTGGCCAGC GTGACGTGGG CTGATGGTAC CACCAATGTG 
TACCGTGTGG GCCACAAGGG CAAGGTGGAC CTCAAGTGTG TGGGCGAGGC AGCGGGCGGC 
TTCTACTACA AGGACCACCT CCCAAGGCTC GGCAAGCCGG CGGAGCTGCA GCGCAGGGTG 
AGTGCTGACA GCCAGCCCTT CCAGCACGGG GACAAGGTCA AGTGTCTGCT GGACACTGAT 
GTCCTGCGGG AGATGCAGGA AGGCCACGGC GGCTGGAACC CCAGGATGGC GGAGTTTATC 
GGACAGACGG GCACCGTGCA TCGTATCACG GACCGCGGGG ACGTGCGCGT GCAGTTCAAC 
CACGAGACGC GCTGGACCTT CCACCCCGGG GCGCTCACCA AGGACACCAG GAAGGCAGGA 
CAGCTTCGTG GGCGGGAGGG AGGCGGCTGG GCTAAGATGC TCCTGCACCA CTCCTTCTGG 
GTGGGCGACG TGGTCCGGGT CATCGGCGAC CTTGACACAG TGAAGCGGCT GCAGGCTGGG 
CATGGCGAGT GGACGGACGA CATGGCCCCT GCCCTGGGCC GCGTCGGGAA GGTGGTGAAA 
GTGTTTGGAG ACGGGAACCT GCGTGTAGCA GTCGCTGGTC AGCGGTGGAC CTTCAGCCCC 
TCCTGCCTGG TGGCCTACCG GCCCGAGGAG GATGCCAACC TGGACGTGGC CGAGCGCGCC 
CGGGAGAACA AAAGCTCACT GAGCGTGGCC CTGGACAAGC TTCGGGCCCA GAAGAGTGAC 
CCAGAGCACC CGGGAAGGCT GGTGGTGGAG GTGGCGCTGG GTAACGCAGC CCGGGCTCTG 
GACCTGCTGC GGAGGCGCCC AGAGCAGGTG GACACCAAGA ACCAAGGCAG GACCGCTCTG 
CAAGTGGCTG CCTACCTGGG CCAGGTGGAG TTGATACGGC TGCTGCTACA AGCCAGGGCG 
GGCGTGGACC TGCCGGACGA CGAGGGCAAC ACGGCACTGC ACTACGCGGC CCTGGGGAAC 
CAGCCCGAGG CCACCAGGGT GCTCCTGAGT GCTGGGTGCC GGGCGGACGC CATCAACAGC 
ACCCAGAGCA CAGCACTGCA CGTGGCCGTG CAGAGGGGCT TCCTGGAGGT GGTGCGGGCC 
CTGTGTGAGC GCGGCTGTGA CGTCAACCTG CCCGACGCCC ACTCGGACAC GCCCCTGCAC 
TCCGCCATCT CGGCGGGCAC TGGAGCCAGC GGCATTGTCG AGGTCCTCAC GGAGGTGCCA 
AACATCGATG TTACCGCCAC CAACAGCCAG GGTTTCACCC TGCTGCACCA TGCCTCCCTC 
AAGGGTCACG CGCTAGCTGT GAGAAAGATT CTGGCTCGGG CGCGGCAGCT GGTGGACGCC 
AAGAAGGAGG ACGGCTTCAC GGCGCTGCAT CTGGCTGCCC TCAACAACCA CCGCGAGGTG 
GCCCAGATCC TCATCCGGGA GGGCCGCTGT GACGTGAACG TGCGCAACCG GAAGCTGCAG 
TCCCCGCTGC ATCTCGCCGT GCAACAGGCC CACGTGGGGC TGGTGCCGCT ACTGGTGGAC 
GCTGGGTGCA GTGTCAACGC CGAGGACGAG GAGGGGGACA CAGCCCTGCA CGTGGCGCTG 
CAGCGTCATC AGCTGCTGCC CCTGGTGGCT GATGGGGCCG GGGGGGACCC AGGGCCCTTG 
CAGCTGCTGT CCAGGACATG GGGCGCCGGC TGCTGGGGCT GCCAGGTGCC AGGAGACGCC 
TCCCTCGGGC CTGCCCCGGC GCCCGCCCTC ACCGGCGTCT GTCCTGCCGC CCAGCTACAG 
GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG 
GCGCTGGAGG GCGCCGACGT GAGCTACACC AACCACCGCG GTCGGAGCCC GCTGGACCTG 
GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG 
CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC AGGCAAACGC TCGGGACCCC CAACACCGTG 
ACGAACCTGC ACGTGGGCGC CGCGCCGGGG CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG TGCCAGCACC GCACCGTGTG TGAGGGTGAG 
TGGGGGGCCC CGGGGTGGGG AGGCCCGGCT AGTAGGGCCG CAGCCAACCG CGCTCTCCTC 
TTCGCAGAGT GCGCGCGCAG GATGAAGAAG TGCATCAGGT GCCAGGTGGT CGTCAGCAAG 
AAACTGCGCC CAGACGGCTC TGAGGTGGCG AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC 
CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAACGCAT CACCTGCCCC 
ATCTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCACGGCGC ATGCGCCCCC 
TGCGGCTCCG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA CCGCATCCAG 
ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855841.1
CDS	273..4124
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000352.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000352.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCCGCCCAAC ATGGACCCAG 
ACCCCCAGGC GGGCGTGCAG GTGGGCATGC GGGTGGTGCG CGGCGTGGAC TGGAAGTGGG 
GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTTGGC CGCCACGGCA 
GCCCCTCGAC ACCCGACCGC ACAGTGGTCG TGCAGTGGGA CCAGGGCACG CGCACCAACT 
ACCGCGCCGG CTACCAGGGC GCGCACGACC TGCTGCTGCG TCCGGCACCC CAACATCATC 
TGTGACTGCT GCAAGAAGCA CGGGCTGCGG GGGATGCGCT GGAAGTGCCG TGTGTGCCTG 
GACTACGACC TCTGCACGCA GTGCTACATG CACAACAAGC ATGAGCTCGC CCACGCCTTC 
GACCGCTACG AGACCGCTCA CTCGCGCCCT GTCACACTGA GTCCCCGCCA GGGCCTCCCG 
AGGATCCCAC TAAGGGGCAT CTTCCAGGGA GCGAAGGTGG TGCGAGGCCC CGACTGGGAG 
TGGGGCTCAC AGGATGGAGG GGAAGGGAAA CCGGGCCGTG TGGTGGACAT CCGTGGCTGG 
GATGTGGAGA CAGGCCGGAG TGTGGCCAGC GTGACGTGGG CTGATGGTAC CACCAATGTG 
TACCGTGTGG GCCACAAGGG CAAGGTGGAC CTCAAGTGTG TGGGCGAGGC AGCGGGCGGC 
TTCTACTACA AGGACCACCT CCCAAGGCTC GGCAAGCCGG CGGAGCTGCA GCGCAGGGTG 
AGTGCTGACA GCCAGCCCTT CCAGCACGGG GACAAGGTCA AGTGTCTGCT GGACACTGAT 
GTCCTGCGGG AGATGCAGGA AGGCCACGGC GGCTGGAACC CCAGGATGGC GGAGTTTATC 
GGACAGACGG GCACCGTGCA TCGTATCACG GACCGCGGGG ACGTGCGCGT GCAGTTCAAC 
CACGAGACGC GCTGGACCTT CCACCCCGGG GCGCTCACCA AGGACACCAG GAAGGCAGGA 
CAGCTTCGTG GGCGGGAGGG AGGCGGCTGG GCTAAGATGC TCCTGCACCA CTCCTTCTGG 
GTGGGCGACG TGGTCCGGGT CATCGGCGAC CTTGACACAG TGAAGCGGCT GCAGGCTGGG 
CATGGCGAGT GGACGGACGA CATGGCCCCT GCCCTGGGCC GCGTCGGGAA GGTGGTGAAA 
GTGTTTGGAG ACGGGAACCT GCGTGTAGCA GTCGCTGGTC AGCGGTGGAC CTTCAGCCCC 
TCCTGCCTGG TGGCCTACCG GCCCGAGGAG GATGCCAACC TGGACGTGGC CGAGCGCGCC 
CGGGAGAACA AAAGCTCACT GAGCGTGGCC CTGGACAAGC TTCGGGCCCA GAAGAGTGAC 
CCAGAGCACC CGGGAAGGCT GGTGGTGGAG GTGGCGCTGG GTAACGCAGC CCGGGCTCTG 
GACCTGCTGC GGAGGCGCCC AGAGCAGGTG GACACCAAGA ACCAAGGCAG GACCGCTCTG 
CAAGTGGCTG CCTACCTGGG CCAGGTGGAG TTGATACGGC TGCTGCTACA AGCCAGGGCG 
GGCGTGGACC TGCCGGACGA CGAGGGCAAC ACGGCACTGC ACTACGCGGC CCTGGGGAAC 
CAGCCCGAGG CCACCAGGGT GCTCCTGAGT GCTGGGTGCC GGGCGGACGC CATCAACAGC 
ACCCAGAGCA CAGCACTGCA CGTGGCCGTG CAGAGGGGCT TCCTGGAGGT GGTGCGGGCC 
CTGTGTGAGC GCGGCTGTGA CGTCAACCTG CCCGACGCCC ACTCGGACAC GCCCCTGCAC 
TCCGCCATCT CGGCGGGCAC TGGAGCCAGC GGCATTGTCG AGGTCCTCAC GGAGGTGCCA 
AACATCGATG TTACCGCCAC CAACAGCCAG GGTTTCACCC TGCTGCACCA TGCCTCCCTC 
AAGGGTCACG CGCTAGCTGT GAGAAAGATT CTGGCTCGGG CGCGGCAGCT GGTGGACGCC 
AAGAAGGAGG ACGGCTTCAC GGCGCTGCAT CTGGCTGCCC TCAACAACCA CCGCGAGGTG 
GCCCAGATCC TCATCCGGGA GGGCCGCTGT GACGTGAACG TGCGCAACCG GAAGCTGCAG 
TCCCCGCTGC ATCTCGCCGT GCAACAGGCC CACGTGGGGC TGGTGCCGCT ACTGGTGGAC 
GCTGGGTGCA GTGTCAACGC CGAGGACGAG GAGGGGGACA CAGCCCTGCA CGTGGCGCTG 
CAGCGTCATC AGCTGCTGCC CCTGGTGGCT GATGGGGCCG GGGGGGACCC AGGGCCCTTG 
CAGCTGCTGT CCAGGACATG GGGCGCCGGC TGCTGGGGCT GCCAGGTGCC AGGAGACGCC 
TCCCTCGGGC CTGCCCCGGC GCCCGCCCTC ACCGGCGTCT GTCCTGCCGC CCAGCTACAG 
GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG 
GCGCTGGAGG GCGCCGACGT GAGCTACACC AACCACCGCG GTCGGAGCCC GCTGGACCTG 
GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG 
CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC AGGCAAACGC TCGGGACCCC CAACACCGTG 
ACGAACCTGC ACGTGGGCGC CGCGCCGGGG CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG TGCCAGCACC GCACCGTGTG TGAGGGTGAG 
TGGGGGGCCC CGGGGTGGGG AGGCCCGGCT AGTAGGGCCG CAGCCAACCG CGCTCTCCTC 
TTCGCAGAGT GCGCGCGCAG GATGAAGAAG TGCATCAGGT GCCAGGTGGT CGTCAGCAAG 
AAACTGCGCC CAGACGGCTC TGAGGTGGCG AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC 
CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAACGCAT CACCTGCCCC 
ATCTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCACGGCGC ATGCGCCCCC 
TGCGGCTCCG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA CCGCATCCAG 
ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000352.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855841.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.

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CloneID	OHu83161
Clone ID Related Accession (Same CDS sequence)	XM_017000352.1 , XM_017000352.2
Accession Version	XM_017000352.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000352.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCCGCCCAAC ATGGACCCAG 
ACCCCCAGGC GGGCGTGCAG GTGGGCATGC GGGTGGTGCG CGGCGTGGAC TGGAAGTGGG 
GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTTGGC CGCCACGGCA 
GCCCCTCGAC ACCCGACCGC ACAGTGGTCG TGCAGTGGGA CCAGGGCACG CGCACCAACT 
ACCGCGCCGG CTACCAGGGC GCGCACGACC TGCTGCTGCG TCCGGCACCC CAACATCATC 
TGTGACTGCT GCAAGAAGCA CGGGCTGCGG GGGATGCGCT GGAAGTGCCG TGTGTGCCTG 
GACTACGACC TCTGCACGCA GTGCTACATG CACAACAAGC ATGAGCTCGC CCACGCCTTC 
GACCGCTACG AGACCGCTCA CTCGCGCCCT GTCACACTGA GTCCCCGCCA GGGCCTCCCG 
AGGATCCCAC TAAGGGGCAT CTTCCAGGGA GCGAAGGTGG TGCGAGGCCC CGACTGGGAG 
TGGGGCTCAC AGGATGGAGG GGAAGGGAAA CCGGGCCGTG TGGTGGACAT CCGTGGCTGG 
GATGTGGAGA CAGGCCGGAG TGTGGCCAGC GTGACGTGGG CTGATGGTAC CACCAATGTG 
TACCGTGTGG GCCACAAGGG CAAGGTGGAC CTCAAGTGTG TGGGCGAGGC AGCGGGCGGC 
TTCTACTACA AGGACCACCT CCCAAGGCTC GGCAAGCCGG CGGAGCTGCA GCGCAGGGTG 
AGTGCTGACA GCCAGCCCTT CCAGCACGGG GACAAGGTCA AGTGTCTGCT GGACACTGAT 
GTCCTGCGGG AGATGCAGGA AGGCCACGGC GGCTGGAACC CCAGGATGGC GGAGTTTATC 
GGACAGACGG GCACCGTGCA TCGTATCACG GACCGCGGGG ACGTGCGCGT GCAGTTCAAC 
CACGAGACGC GCTGGACCTT CCACCCCGGG GCGCTCACCA AGGACACCAG GAAGGCAGGA 
CAGCTTCGTG GGCGGGAGGG AGGCGGCTGG GCTAAGATGC TCCTGCACCA CTCCTTCTGG 
GTGGGCGACG TGGTCCGGGT CATCGGCGAC CTTGACACAG TGAAGCGGCT GCAGGCTGGG 
CATGGCGAGT GGACGGACGA CATGGCCCCT GCCCTGGGCC GCGTCGGGAA GGTGGTGAAA 
GTGTTTGGAG ACGGGAACCT GCGTGTAGCA GTCGCTGGTC AGCGGTGGAC CTTCAGCCCC 
TCCTGCCTGG TGGCCTACCG GCCCGAGGAG GATGCCAACC TGGACGTGGC CGAGCGCGCC 
CGGGAGAACA AAAGCTCACT GAGCGTGGCC CTGGACAAGC TTCGGGCCCA GAAGAGTGAC 
CCAGAGCACC CGGGAAGGCT GGTGGTGGAG GTGGCGCTGG GTAACGCAGC CCGGGCTCTG 
GACCTGCTGC GGAGGCGCCC AGAGCAGGTG GACACCAAGA ACCAAGGCAG GACCGCTCTG 
CAAGTGGCTG CCTACCTGGG CCAGGTGGAG TTGATACGGC TGCTGCTACA AGCCAGGGCG 
GGCGTGGACC TGCCGGACGA CGAGGGCAAC ACGGCACTGC ACTACGCGGC CCTGGGGAAC 
CAGCCCGAGG CCACCAGGGT GCTCCTGAGT GCTGGGTGCC GGGCGGACGC CATCAACAGC 
ACCCAGAGCA CAGCACTGCA CGTGGCCGTG CAGAGGGGCT TCCTGGAGGT GGTGCGGGCC 
CTGTGTGAGC GCGGCTGTGA CGTCAACCTG CCCGACGCCC ACTCGGACAC GCCCCTGCAC 
TCCGCCATCT CGGCGGGCAC TGGAGCCAGC GGCATTGTCG AGGTCCTCAC GGAGGTGCCA 
AACATCGATG TTACCGCCAC CAACAGCCAG GGTTTCACCC TGCTGCACCA TGCCTCCCTC 
AAGGGTCACG CGCTAGCTGT GAGAAAGATT CTGGCTCGGG CGCGGCAGCT GGTGGACGCC 
AAGAAGGAGG ACGGCTTCAC GGCGCTGCAT CTGGCTGCCC TCAACAACCA CCGCGAGGTG 
GCCCAGATCC TCATCCGGGA GGGCCGCTGT GACGTGAACG TGCGCAACCG GAAGCTGCAG 
TCCCCGCTGC ATCTCGCCGT GCAACAGGCC CACGTGGGGC TGGTGCCGCT ACTGGTGGAC 
GCTGGGTGCA GTGTCAACGC CGAGGACGAG GAGGGGGACA CAGCCCTGCA CGTGGCGCTG 
CAGCGTCATC AGCTGCTGCC CCTGGTGGCT GATGGGGCCG GGGGGGACCC AGGGCCCTTG 
CAGCTGCTGT CCAGGACATG GGGCGCCGGC TGCTGGGGCT GCCAGGTGCC AGGAGACGCC 
TCCCTCGGGC CTGCCCCGGC GCCCGCCCTC ACCGGCGTCT GTCCTGCCGC CCAGCTACAG 
GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG 
GCGCTGGAGG GCGCCGACGT GAGCTACACC AACCACCGCG GTCGGAGCCC GCTGGACCTG 
GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG 
CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC AGGCAAACGC TCGGGACCCC CAACACCGTG 
ACGAACCTGC ACGTGGGCGC CGCGCCGGGG CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG TGCCAGCACC GCACCGTGTG TGAGGGTGAG 
TGGGGGGCCC CGGGGTGGGG AGGCCCGGCT AGTAGGGCCG CAGCCAACCG CGCTCTCCTC 
TTCGCAGAGT GCGCGCGCAG GATGAAGAAG TGCATCAGGT GCCAGGTGGT CGTCAGCAAG 
AAACTGCGCC CAGACGGCTC TGAGGTGGCG AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC 
CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAACGCAT CACCTGCCCC 
ATCTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCACGGCGC ATGCGCCCCC 
TGCGGCTCCG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA CCGCATCCAG 
ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855841.1
CDS	1723..5574
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000352.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000352.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCCGCCCAAC ATGGACCCAG 
ACCCCCAGGC GGGCGTGCAG GTGGGCATGC GGGTGGTGCG CGGCGTGGAC TGGAAGTGGG 
GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTTGGC CGCCACGGCA 
GCCCCTCGAC ACCCGACCGC ACAGTGGTCG TGCAGTGGGA CCAGGGCACG CGCACCAACT 
ACCGCGCCGG CTACCAGGGC GCGCACGACC TGCTGCTGCG TCCGGCACCC CAACATCATC 
TGTGACTGCT GCAAGAAGCA CGGGCTGCGG GGGATGCGCT GGAAGTGCCG TGTGTGCCTG 
GACTACGACC TCTGCACGCA GTGCTACATG CACAACAAGC ATGAGCTCGC CCACGCCTTC 
GACCGCTACG AGACCGCTCA CTCGCGCCCT GTCACACTGA GTCCCCGCCA GGGCCTCCCG 
AGGATCCCAC TAAGGGGCAT CTTCCAGGGA GCGAAGGTGG TGCGAGGCCC CGACTGGGAG 
TGGGGCTCAC AGGATGGAGG GGAAGGGAAA CCGGGCCGTG TGGTGGACAT CCGTGGCTGG 
GATGTGGAGA CAGGCCGGAG TGTGGCCAGC GTGACGTGGG CTGATGGTAC CACCAATGTG 
TACCGTGTGG GCCACAAGGG CAAGGTGGAC CTCAAGTGTG TGGGCGAGGC AGCGGGCGGC 
TTCTACTACA AGGACCACCT CCCAAGGCTC GGCAAGCCGG CGGAGCTGCA GCGCAGGGTG 
AGTGCTGACA GCCAGCCCTT CCAGCACGGG GACAAGGTCA AGTGTCTGCT GGACACTGAT 
GTCCTGCGGG AGATGCAGGA AGGCCACGGC GGCTGGAACC CCAGGATGGC GGAGTTTATC 
GGACAGACGG GCACCGTGCA TCGTATCACG GACCGCGGGG ACGTGCGCGT GCAGTTCAAC 
CACGAGACGC GCTGGACCTT CCACCCCGGG GCGCTCACCA AGGACACCAG GAAGGCAGGA 
CAGCTTCGTG GGCGGGAGGG AGGCGGCTGG GCTAAGATGC TCCTGCACCA CTCCTTCTGG 
GTGGGCGACG TGGTCCGGGT CATCGGCGAC CTTGACACAG TGAAGCGGCT GCAGGCTGGG 
CATGGCGAGT GGACGGACGA CATGGCCCCT GCCCTGGGCC GCGTCGGGAA GGTGGTGAAA 
GTGTTTGGAG ACGGGAACCT GCGTGTAGCA GTCGCTGGTC AGCGGTGGAC CTTCAGCCCC 
TCCTGCCTGG TGGCCTACCG GCCCGAGGAG GATGCCAACC TGGACGTGGC CGAGCGCGCC 
CGGGAGAACA AAAGCTCACT GAGCGTGGCC CTGGACAAGC TTCGGGCCCA GAAGAGTGAC 
CCAGAGCACC CGGGAAGGCT GGTGGTGGAG GTGGCGCTGG GTAACGCAGC CCGGGCTCTG 
GACCTGCTGC GGAGGCGCCC AGAGCAGGTG GACACCAAGA ACCAAGGCAG GACCGCTCTG 
CAAGTGGCTG CCTACCTGGG CCAGGTGGAG TTGATACGGC TGCTGCTACA AGCCAGGGCG 
GGCGTGGACC TGCCGGACGA CGAGGGCAAC ACGGCACTGC ACTACGCGGC CCTGGGGAAC 
CAGCCCGAGG CCACCAGGGT GCTCCTGAGT GCTGGGTGCC GGGCGGACGC CATCAACAGC 
ACCCAGAGCA CAGCACTGCA CGTGGCCGTG CAGAGGGGCT TCCTGGAGGT GGTGCGGGCC 
CTGTGTGAGC GCGGCTGTGA CGTCAACCTG CCCGACGCCC ACTCGGACAC GCCCCTGCAC 
TCCGCCATCT CGGCGGGCAC TGGAGCCAGC GGCATTGTCG AGGTCCTCAC GGAGGTGCCA 
AACATCGATG TTACCGCCAC CAACAGCCAG GGTTTCACCC TGCTGCACCA TGCCTCCCTC 
AAGGGTCACG CGCTAGCTGT GAGAAAGATT CTGGCTCGGG CGCGGCAGCT GGTGGACGCC 
AAGAAGGAGG ACGGCTTCAC GGCGCTGCAT CTGGCTGCCC TCAACAACCA CCGCGAGGTG 
GCCCAGATCC TCATCCGGGA GGGCCGCTGT GACGTGAACG TGCGCAACCG GAAGCTGCAG 
TCCCCGCTGC ATCTCGCCGT GCAACAGGCC CACGTGGGGC TGGTGCCGCT ACTGGTGGAC 
GCTGGGTGCA GTGTCAACGC CGAGGACGAG GAGGGGGACA CAGCCCTGCA CGTGGCGCTG 
CAGCGTCATC AGCTGCTGCC CCTGGTGGCT GATGGGGCCG GGGGGGACCC AGGGCCCTTG 
CAGCTGCTGT CCAGGACATG GGGCGCCGGC TGCTGGGGCT GCCAGGTGCC AGGAGACGCC 
TCCCTCGGGC CTGCCCCGGC GCCCGCCCTC ACCGGCGTCT GTCCTGCCGC CCAGCTACAG 
GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG 
GCGCTGGAGG GCGCCGACGT GAGCTACACC AACCACCGCG GTCGGAGCCC GCTGGACCTG 
GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG 
CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC AGGCAAACGC TCGGGACCCC CAACACCGTG 
ACGAACCTGC ACGTGGGCGC CGCGCCGGGG CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC 
GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG TGCCAGCACC GCACCGTGTG TGAGGGTGAG 
TGGGGGGCCC CGGGGTGGGG AGGCCCGGCT AGTAGGGCCG CAGCCAACCG CGCTCTCCTC 
TTCGCAGAGT GCGCGCGCAG GATGAAGAAG TGCATCAGGT GCCAGGTGGT CGTCAGCAAG 
AAACTGCGCC CAGACGGCTC TGAGGTGGCG AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC 
CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC CGGCAGATGG AGGAACGCAT CACCTGCCCC 
ATCTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCACGGCGC ATGCGCCCCC 
TGCGGCTCCG CGCTCAGCGC CTGCCCCATC TGCCGCCAGC CCATCCGCGA CCGCATCCAG 
ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000353.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855842.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu83162
Clone ID Related Accession (Same CDS sequence)	XM_017000353.1 , XM_017000353.2
Accession Version	XM_017000353.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGCACCAC TCCTTCTGGG TGGGCGACGT GGTCCGGGTC 
ATCGGCGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACGGACGAC 
ATGGCCCCTG CCCTGGGCCG CGTCGGGAAG GTGGTGAAAG TGTTTGGAGA CGGGAACCTG 
CGTGTAGCAG TCGCTGGTCA GCGGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCCGAGGAGG ATGCCAACCT GGACGTGGCC GAGCGCGCCC GGGAGAACAA AAGCTCACTG 
AGCGTGGCCC TGGACAAGCT TCGGGCCCAG AAGAGTGACC CAGAGCACCC GGGAAGGCTG 
GTGGTGGAGG TGGCGCTGGG TAACGCAGCC CGGGCTCTGG ACCTGCTGCG GAGGCGCCCA 
GAGCAGGTGG ACACCAAGAA CCAAGGCAGG ACCGCTCTGC AAGTGGCTGC CTACCTGGGC 
CAGGTGGAGT TGATACGGCT GCTGCTACAA GCCAGGGCGG GCGTGGACCT GCCGGACGAC 
GAGGGCAACA CGGCACTGCA CTACGCGGCC CTGGGGAACC AGCCCGAGGC CACCAGGGTG 
CTCCTGAGTG CTGGGTGCCG GGCGGACGCC ATCAACAGCA CCCAGAGCAC AGCACTGCAC 
GTGGCCGTGC AGAGGGGCTT CCTGGAGGTG GTGCGGGCCC TGTGTGAGCG CGGCTGTGAC 
GTCAACCTGC CCGACGCCCA CTCGGACACG CCCCTGCACT CCGCCATCTC GGCGGGCACT 
GGAGCCAGCG GCATTGTCGA GGTCCTCACG GAGGTGCCAA ACATCGATGT TACCGCCACC 
AACAGCCAGG GTTTCACCCT GCTGCACCAT GCCTCCCTCA AGGGTCACGC GCTAGCTGTG 
AGAAAGATTC TGGCTCGGGC GCGGCAGCTG GTGGACGCCA AGAAGGAGGA CGGCTTCACG 
GCGCTGCATC TGGCTGCCCT CAACAACCAC CGCGAGGTGG CCCAGATCCT CATCCGGGAG 
GGCCGCTGTG ACGTGAACGT GCGCAACCGG AAGCTGCAGT CCCCGCTGCA TCTCGCCGTG 
CAACAGGCCC ACGTGGGGCT GGTGCCGCTA CTGGTGGACG CTGGGTGCAG TGTCAACGCC 
GAGGACGAGG AGGGGGACAC AGCCCTGCAC GTGGCGCTGC AGCGTCATCA GCTGCTGCCC 
CTGGTGGCTG ATGGGGCCGG GGGGGACCCA GGGCCCTTGC AGCTGCTGTC CAGGACATGG 
GGCGCCGGCT GCTGGGGCTG CCAGGTGCCA GGAGACGCCT CCCTCGGGCC TGCCCCGGCG 
CCCGCCCTCA CCGGCGTCTG TCCTGCCGCC CAGCTACAGG CCTCGGGCCT CCCCGGCAGC 
GCGGAGCTGA CGGTGGGCGC GGCGGTCGCC TGCTTCCTGG CGCTGGAGGG CGCCGACGTG 
AGCTACACCA ACCACCGCGG TCGGAGCCCG CTGGACCTGG CCGCCGAGGG TCGCGTGCTC 
AAGGCCCTTC AGGGCTGCGC CCAGCGCTTC CGGGAGCGGC AGGCGGGCGG GGGCGCGGCC 
CCGGGCCCCA GGCAAACGCT CGGGACCCCC AACACCGTGA CGAACCTGCA CGTGGGCGCC 
GCGCCGGGGC CCGAGGCCGC TGAGTGCCTG GTGTGCTCCG AGCTGGCGCT GCTGGTGCTG 
TTCTCGCCGT GCCAGCACCG CACCGTGTGT GAGGGTGAGT GGGGGGCCCC GGGGTGGGGA 
GGCCCGGCTA GTAGGGCCGC AGCCAACCGC GCTCTCCTCT TCGCAGAGTG CGCGCGCAGG 
ATGAAGAAGT GCATCAGGTG CCAGGTGGTC GTCAGCAAGA AACTGCGCCC AGACGGCTCT 
GAGGTGGCGA GCGCCGCCCC CGCCCCCGGC CCGCCGCGCC AGCTGGTGGA GGAGCTGCAG 
AGCCGCTACC GGCAGATGGA GGAACGCATC ACCTGCCCCA TCTGCATCGA CAGCCACATC 
CGCCTCGTGT TCCAGTGCGG CCACGGCGCA TGCGCCCCCT GCGGCTCCGC GCTCAGCGCC 
TGCCCCATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855842.1
CDS	273..4103
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000353.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000353.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGCACCAC TCCTTCTGGG TGGGCGACGT GGTCCGGGTC 
ATCGGCGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACGGACGAC 
ATGGCCCCTG CCCTGGGCCG CGTCGGGAAG GTGGTGAAAG TGTTTGGAGA CGGGAACCTG 
CGTGTAGCAG TCGCTGGTCA GCGGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCCGAGGAGG ATGCCAACCT GGACGTGGCC GAGCGCGCCC GGGAGAACAA AAGCTCACTG 
AGCGTGGCCC TGGACAAGCT TCGGGCCCAG AAGAGTGACC CAGAGCACCC GGGAAGGCTG 
GTGGTGGAGG TGGCGCTGGG TAACGCAGCC CGGGCTCTGG ACCTGCTGCG GAGGCGCCCA 
GAGCAGGTGG ACACCAAGAA CCAAGGCAGG ACCGCTCTGC AAGTGGCTGC CTACCTGGGC 
CAGGTGGAGT TGATACGGCT GCTGCTACAA GCCAGGGCGG GCGTGGACCT GCCGGACGAC 
GAGGGCAACA CGGCACTGCA CTACGCGGCC CTGGGGAACC AGCCCGAGGC CACCAGGGTG 
CTCCTGAGTG CTGGGTGCCG GGCGGACGCC ATCAACAGCA CCCAGAGCAC AGCACTGCAC 
GTGGCCGTGC AGAGGGGCTT CCTGGAGGTG GTGCGGGCCC TGTGTGAGCG CGGCTGTGAC 
GTCAACCTGC CCGACGCCCA CTCGGACACG CCCCTGCACT CCGCCATCTC GGCGGGCACT 
GGAGCCAGCG GCATTGTCGA GGTCCTCACG GAGGTGCCAA ACATCGATGT TACCGCCACC 
AACAGCCAGG GTTTCACCCT GCTGCACCAT GCCTCCCTCA AGGGTCACGC GCTAGCTGTG 
AGAAAGATTC TGGCTCGGGC GCGGCAGCTG GTGGACGCCA AGAAGGAGGA CGGCTTCACG 
GCGCTGCATC TGGCTGCCCT CAACAACCAC CGCGAGGTGG CCCAGATCCT CATCCGGGAG 
GGCCGCTGTG ACGTGAACGT GCGCAACCGG AAGCTGCAGT CCCCGCTGCA TCTCGCCGTG 
CAACAGGCCC ACGTGGGGCT GGTGCCGCTA CTGGTGGACG CTGGGTGCAG TGTCAACGCC 
GAGGACGAGG AGGGGGACAC AGCCCTGCAC GTGGCGCTGC AGCGTCATCA GCTGCTGCCC 
CTGGTGGCTG ATGGGGCCGG GGGGGACCCA GGGCCCTTGC AGCTGCTGTC CAGGACATGG 
GGCGCCGGCT GCTGGGGCTG CCAGGTGCCA GGAGACGCCT CCCTCGGGCC TGCCCCGGCG 
CCCGCCCTCA CCGGCGTCTG TCCTGCCGCC CAGCTACAGG CCTCGGGCCT CCCCGGCAGC 
GCGGAGCTGA CGGTGGGCGC GGCGGTCGCC TGCTTCCTGG CGCTGGAGGG CGCCGACGTG 
AGCTACACCA ACCACCGCGG TCGGAGCCCG CTGGACCTGG CCGCCGAGGG TCGCGTGCTC 
AAGGCCCTTC AGGGCTGCGC CCAGCGCTTC CGGGAGCGGC AGGCGGGCGG GGGCGCGGCC 
CCGGGCCCCA GGCAAACGCT CGGGACCCCC AACACCGTGA CGAACCTGCA CGTGGGCGCC 
GCGCCGGGGC CCGAGGCCGC TGAGTGCCTG GTGTGCTCCG AGCTGGCGCT GCTGGTGCTG 
TTCTCGCCGT GCCAGCACCG CACCGTGTGT GAGGGTGAGT GGGGGGCCCC GGGGTGGGGA 
GGCCCGGCTA GTAGGGCCGC AGCCAACCGC GCTCTCCTCT TCGCAGAGTG CGCGCGCAGG 
ATGAAGAAGT GCATCAGGTG CCAGGTGGTC GTCAGCAAGA AACTGCGCCC AGACGGCTCT 
GAGGTGGCGA GCGCCGCCCC CGCCCCCGGC CCGCCGCGCC AGCTGGTGGA GGAGCTGCAG 
AGCCGCTACC GGCAGATGGA GGAACGCATC ACCTGCCCCA TCTGCATCGA CAGCCACATC 
CGCCTCGTGT TCCAGTGCGG CCACGGCGCA TGCGCCCCCT GCGGCTCCGC GCTCAGCGCC 
TGCCCCATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000353.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855842.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu83162
Clone ID Related Accession (Same CDS sequence)	XM_017000353.1 , XM_017000353.2
Accession Version	XM_017000353.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3831bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000353.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGCACCAC TCCTTCTGGG TGGGCGACGT GGTCCGGGTC 
ATCGGCGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACGGACGAC 
ATGGCCCCTG CCCTGGGCCG CGTCGGGAAG GTGGTGAAAG TGTTTGGAGA CGGGAACCTG 
CGTGTAGCAG TCGCTGGTCA GCGGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCCGAGGAGG ATGCCAACCT GGACGTGGCC GAGCGCGCCC GGGAGAACAA AAGCTCACTG 
AGCGTGGCCC TGGACAAGCT TCGGGCCCAG AAGAGTGACC CAGAGCACCC GGGAAGGCTG 
GTGGTGGAGG TGGCGCTGGG TAACGCAGCC CGGGCTCTGG ACCTGCTGCG GAGGCGCCCA 
GAGCAGGTGG ACACCAAGAA CCAAGGCAGG ACCGCTCTGC AAGTGGCTGC CTACCTGGGC 
CAGGTGGAGT TGATACGGCT GCTGCTACAA GCCAGGGCGG GCGTGGACCT GCCGGACGAC 
GAGGGCAACA CGGCACTGCA CTACGCGGCC CTGGGGAACC AGCCCGAGGC CACCAGGGTG 
CTCCTGAGTG CTGGGTGCCG GGCGGACGCC ATCAACAGCA CCCAGAGCAC AGCACTGCAC 
GTGGCCGTGC AGAGGGGCTT CCTGGAGGTG GTGCGGGCCC TGTGTGAGCG CGGCTGTGAC 
GTCAACCTGC CCGACGCCCA CTCGGACACG CCCCTGCACT CCGCCATCTC GGCGGGCACT 
GGAGCCAGCG GCATTGTCGA GGTCCTCACG GAGGTGCCAA ACATCGATGT TACCGCCACC 
AACAGCCAGG GTTTCACCCT GCTGCACCAT GCCTCCCTCA AGGGTCACGC GCTAGCTGTG 
AGAAAGATTC TGGCTCGGGC GCGGCAGCTG GTGGACGCCA AGAAGGAGGA CGGCTTCACG 
GCGCTGCATC TGGCTGCCCT CAACAACCAC CGCGAGGTGG CCCAGATCCT CATCCGGGAG 
GGCCGCTGTG ACGTGAACGT GCGCAACCGG AAGCTGCAGT CCCCGCTGCA TCTCGCCGTG 
CAACAGGCCC ACGTGGGGCT GGTGCCGCTA CTGGTGGACG CTGGGTGCAG TGTCAACGCC 
GAGGACGAGG AGGGGGACAC AGCCCTGCAC GTGGCGCTGC AGCGTCATCA GCTGCTGCCC 
CTGGTGGCTG ATGGGGCCGG GGGGGACCCA GGGCCCTTGC AGCTGCTGTC CAGGACATGG 
GGCGCCGGCT GCTGGGGCTG CCAGGTGCCA GGAGACGCCT CCCTCGGGCC TGCCCCGGCG 
CCCGCCCTCA CCGGCGTCTG TCCTGCCGCC CAGCTACAGG CCTCGGGCCT CCCCGGCAGC 
GCGGAGCTGA CGGTGGGCGC GGCGGTCGCC TGCTTCCTGG CGCTGGAGGG CGCCGACGTG 
AGCTACACCA ACCACCGCGG TCGGAGCCCG CTGGACCTGG CCGCCGAGGG TCGCGTGCTC 
AAGGCCCTTC AGGGCTGCGC CCAGCGCTTC CGGGAGCGGC AGGCGGGCGG GGGCGCGGCC 
CCGGGCCCCA GGCAAACGCT CGGGACCCCC AACACCGTGA CGAACCTGCA CGTGGGCGCC 
GCGCCGGGGC CCGAGGCCGC TGAGTGCCTG GTGTGCTCCG AGCTGGCGCT GCTGGTGCTG 
TTCTCGCCGT GCCAGCACCG CACCGTGTGT GAGGGTGAGT GGGGGGCCCC GGGGTGGGGA 
GGCCCGGCTA GTAGGGCCGC AGCCAACCGC GCTCTCCTCT TCGCAGAGTG CGCGCGCAGG 
ATGAAGAAGT GCATCAGGTG CCAGGTGGTC GTCAGCAAGA AACTGCGCCC AGACGGCTCT 
GAGGTGGCGA GCGCCGCCCC CGCCCCCGGC CCGCCGCGCC AGCTGGTGGA GGAGCTGCAG 
AGCCGCTACC GGCAGATGGA GGAACGCATC ACCTGCCCCA TCTGCATCGA CAGCCACATC 
CGCCTCGTGT TCCAGTGCGG CCACGGCGCA TGCGCCCCCT GCGGCTCCGC GCTCAGCGCC 
TGCCCCATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855842.1
CDS	1713..5543
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000353.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000353.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGCACCAC TCCTTCTGGG TGGGCGACGT GGTCCGGGTC 
ATCGGCGACC TTGACACAGT GAAGCGGCTG CAGGCTGGGC ATGGCGAGTG GACGGACGAC 
ATGGCCCCTG CCCTGGGCCG CGTCGGGAAG GTGGTGAAAG TGTTTGGAGA CGGGAACCTG 
CGTGTAGCAG TCGCTGGTCA GCGGTGGACC TTCAGCCCCT CCTGCCTGGT GGCCTACCGG 
CCCGAGGAGG ATGCCAACCT GGACGTGGCC GAGCGCGCCC GGGAGAACAA AAGCTCACTG 
AGCGTGGCCC TGGACAAGCT TCGGGCCCAG AAGAGTGACC CAGAGCACCC GGGAAGGCTG 
GTGGTGGAGG TGGCGCTGGG TAACGCAGCC CGGGCTCTGG ACCTGCTGCG GAGGCGCCCA 
GAGCAGGTGG ACACCAAGAA CCAAGGCAGG ACCGCTCTGC AAGTGGCTGC CTACCTGGGC 
CAGGTGGAGT TGATACGGCT GCTGCTACAA GCCAGGGCGG GCGTGGACCT GCCGGACGAC 
GAGGGCAACA CGGCACTGCA CTACGCGGCC CTGGGGAACC AGCCCGAGGC CACCAGGGTG 
CTCCTGAGTG CTGGGTGCCG GGCGGACGCC ATCAACAGCA CCCAGAGCAC AGCACTGCAC 
GTGGCCGTGC AGAGGGGCTT CCTGGAGGTG GTGCGGGCCC TGTGTGAGCG CGGCTGTGAC 
GTCAACCTGC CCGACGCCCA CTCGGACACG CCCCTGCACT CCGCCATCTC GGCGGGCACT 
GGAGCCAGCG GCATTGTCGA GGTCCTCACG GAGGTGCCAA ACATCGATGT TACCGCCACC 
AACAGCCAGG GTTTCACCCT GCTGCACCAT GCCTCCCTCA AGGGTCACGC GCTAGCTGTG 
AGAAAGATTC TGGCTCGGGC GCGGCAGCTG GTGGACGCCA AGAAGGAGGA CGGCTTCACG 
GCGCTGCATC TGGCTGCCCT CAACAACCAC CGCGAGGTGG CCCAGATCCT CATCCGGGAG 
GGCCGCTGTG ACGTGAACGT GCGCAACCGG AAGCTGCAGT CCCCGCTGCA TCTCGCCGTG 
CAACAGGCCC ACGTGGGGCT GGTGCCGCTA CTGGTGGACG CTGGGTGCAG TGTCAACGCC 
GAGGACGAGG AGGGGGACAC AGCCCTGCAC GTGGCGCTGC AGCGTCATCA GCTGCTGCCC 
CTGGTGGCTG ATGGGGCCGG GGGGGACCCA GGGCCCTTGC AGCTGCTGTC CAGGACATGG 
GGCGCCGGCT GCTGGGGCTG CCAGGTGCCA GGAGACGCCT CCCTCGGGCC TGCCCCGGCG 
CCCGCCCTCA CCGGCGTCTG TCCTGCCGCC CAGCTACAGG CCTCGGGCCT CCCCGGCAGC 
GCGGAGCTGA CGGTGGGCGC GGCGGTCGCC TGCTTCCTGG CGCTGGAGGG CGCCGACGTG 
AGCTACACCA ACCACCGCGG TCGGAGCCCG CTGGACCTGG CCGCCGAGGG TCGCGTGCTC 
AAGGCCCTTC AGGGCTGCGC CCAGCGCTTC CGGGAGCGGC AGGCGGGCGG GGGCGCGGCC 
CCGGGCCCCA GGCAAACGCT CGGGACCCCC AACACCGTGA CGAACCTGCA CGTGGGCGCC 
GCGCCGGGGC CCGAGGCCGC TGAGTGCCTG GTGTGCTCCG AGCTGGCGCT GCTGGTGCTG 
TTCTCGCCGT GCCAGCACCG CACCGTGTGT GAGGGTGAGT GGGGGGCCCC GGGGTGGGGA 
GGCCCGGCTA GTAGGGCCGC AGCCAACCGC GCTCTCCTCT TCGCAGAGTG CGCGCGCAGG 
ATGAAGAAGT GCATCAGGTG CCAGGTGGTC GTCAGCAAGA AACTGCGCCC AGACGGCTCT 
GAGGTGGCGA GCGCCGCCCC CGCCCCCGGC CCGCCGCGCC AGCTGGTGGA GGAGCTGCAG 
AGCCGCTACC GGCAGATGGA GGAACGCATC ACCTGCCCCA TCTGCATCGA CAGCCACATC 
CGCCTCGTGT TCCAGTGCGG CCACGGCGCA TGCGCCCCCT GCGGCTCCGC GCTCAGCGCC 
TGCCCCATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA TCTTCGTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000354.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855843.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.

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CloneID	OHu83163
Clone ID Related Accession (Same CDS sequence)	XM_017000354.2 , XM_017000354.1
Accession Version	XM_017000354.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000354.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA 
CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG CTGACGGTGG GCGCGGCGGT CGCCTGCTTC 
CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC ACCAACCACC GCGGTCGGAG CCCGCTGGAC 
CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG 
CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC CCCAGGCAAA CGCTCGGGAC CCCCAACACC 
GTGACGAACC TGCACGTGGG CGCCGCGCCG GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC 
TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG CCGTGCCAGC ACCGCACCGT GTGTGAGGGT 
GAGTGGGGGG CCCCGGGGTG GGGAGGCCCG GCTAGTAGGG CCGCAGCCAA CCGCGCTCTC 
CTCTTCGCAG AGTGCGCGCG CAGGATGAAG AAGTGCATCA GGTGCCAGGT GGTCGTCAGC 
AAGAAACTGC GCCCAGACGG CTCTGAGGTG GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG 
CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC TACCGGCAGA TGGAGGAACG CATCACCTGC 
CCCATCTGCA TCGACAGCCA CATCCGCCTC GTGTTCCAGT GCGGCCACGG CGCATGCGCC 
CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC ATCTGCCGCC AGCCCATCCG CGACCGCATC 
CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855843.1
CDS	1716..5510
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000354.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000354.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA 
CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG CTGACGGTGG GCGCGGCGGT CGCCTGCTTC 
CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC ACCAACCACC GCGGTCGGAG CCCGCTGGAC 
CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG 
CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC CCCAGGCAAA CGCTCGGGAC CCCCAACACC 
GTGACGAACC TGCACGTGGG CGCCGCGCCG GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC 
TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG CCGTGCCAGC ACCGCACCGT GTGTGAGGGT 
GAGTGGGGGG CCCCGGGGTG GGGAGGCCCG GCTAGTAGGG CCGCAGCCAA CCGCGCTCTC 
CTCTTCGCAG AGTGCGCGCG CAGGATGAAG AAGTGCATCA GGTGCCAGGT GGTCGTCAGC 
AAGAAACTGC GCCCAGACGG CTCTGAGGTG GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG 
CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC TACCGGCAGA TGGAGGAACG CATCACCTGC 
CCCATCTGCA TCGACAGCCA CATCCGCCTC GTGTTCCAGT GCGGCCACGG CGCATGCGCC 
CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC ATCTGCCGCC AGCCCATCCG CGACCGCATC 
CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000354.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855843.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu83163
Clone ID Related Accession (Same CDS sequence)	XM_017000354.2 , XM_017000354.1
Accession Version	XM_017000354.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA 
CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG CTGACGGTGG GCGCGGCGGT CGCCTGCTTC 
CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC ACCAACCACC GCGGTCGGAG CCCGCTGGAC 
CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG 
CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC CCCAGGCAAA CGCTCGGGAC CCCCAACACC 
GTGACGAACC TGCACGTGGG CGCCGCGCCG GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC 
TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG CCGTGCCAGC ACCGCACCGT GTGTGAGGGT 
GAGTGGGGGG CCCCGGGGTG GGGAGGCCCG GCTAGTAGGG CCGCAGCCAA CCGCGCTCTC 
CTCTTCGCAG AGTGCGCGCG CAGGATGAAG AAGTGCATCA GGTGCCAGGT GGTCGTCAGC 
AAGAAACTGC GCCCAGACGG CTCTGAGGTG GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG 
CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC TACCGGCAGA TGGAGGAACG CATCACCTGC 
CCCATCTGCA TCGACAGCCA CATCCGCCTC GTGTTCCAGT GCGGCCACGG CGCATGCGCC 
CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC ATCTGCCGCC AGCCCATCCG CGACCGCATC 
CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855843.1
CDS	273..4067
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000354.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000354.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGACACC AGGAAGGCAG GACAGCTTCG TGGGCGGGAG 
GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC CACTCCTTCT GGGTGGGCGA CGTGGTCCGG 
GTCATCGGCG ACCTTGACAC AGTGAAGCGG CTGCAGGCTG GGCATGGCGA GTGGACGGAC 
GACATGGCCC CTGCCCTGGG CCGCGTCGGG AAGGTGGTGA AAGTGTTTGG AGACGGGAAC 
CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC 
CGGCCCGAGG AGGATGCCAA CCTGGACGTG GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA 
CTGAGCGTGG CCCTGGACAA GCTTCGGGCC CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG 
CTGGTGGTGG AGGTGGCGCT GGGTAACGCA GCCCGGGCTC TGGACCTGCT GCGGAGGCGC 
CCAGAGCAGG TGGACACCAA GAACCAAGGC AGGACCGCTC TGCAAGTGGC TGCCTACCTG 
GGCCAGGTGG AGTTGATACG GCTGCTGCTA CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC 
GACGAGGGCA ACACGGCACT GCACTACGCG GCCCTGGGGA ACCAGCCCGA GGCCACCAGG 
GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC GCCATCAACA GCACCCAGAG CACAGCACTG 
CACGTGGCCG TGCAGAGGGG CTTCCTGGAG GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT 
GACGTCAACC TGCCCGACGC CCACTCGGAC ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC 
ACTGGAGCCA GCGGCATTGT CGAGGTCCTC ACGGAGGTGC CAAACATCGA TGTTACCGCC 
ACCAACAGCC AGGGTTTCAC CCTGCTGCAC CATGCCTCCC TCAAGGGTCA CGCGCTAGCT 
GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG CTGGTGGACG CCAAGAAGGA GGACGGCTTC 
ACGGCGCTGC ATCTGGCTGC CCTCAACAAC CACCGCGAGG TGGCCCAGAT CCTCATCCGG 
GAGGGCCGCT GTGACGTGAA CGTGCGCAAC CGGAAGCTGC AGTCCCCGCT GCATCTCGCC 
GTGCAACAGG CCCACGTGGG GCTGGTGCCG CTACTGGTGG ACGCTGGGTG CAGTGTCAAC 
GCCGAGGACG AGGAGGGGGA CACAGCCCTG CACGTGGCGC TGCAGCGTCA TCAGCTGCTG 
CCCCTGGTGG CTGATGGGGC CGGGGGGGAC CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA 
CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG CTGACGGTGG GCGCGGCGGT CGCCTGCTTC 
CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC ACCAACCACC GCGGTCGGAG CCCGCTGGAC 
CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG 
CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC CCCAGGCAAA CGCTCGGGAC CCCCAACACC 
GTGACGAACC TGCACGTGGG CGCCGCGCCG GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC 
TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG CCGTGCCAGC ACCGCACCGT GTGTGAGGGT 
GAGTGGGGGG CCCCGGGGTG GGGAGGCCCG GCTAGTAGGG CCGCAGCCAA CCGCGCTCTC 
CTCTTCGCAG AGTGCGCGCG CAGGATGAAG AAGTGCATCA GGTGCCAGGT GGTCGTCAGC 
AAGAAACTGC GCCCAGACGG CTCTGAGGTG GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG 
CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC TACCGGCAGA TGGAGGAACG CATCACCTGC 
CCCATCTGCA TCGACAGCCA CATCCGCCTC GTGTTCCAGT GCGGCCACGG CGCATGCGCC 
CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC ATCTGCCGCC AGCCCATCCG CGACCGCATC 
CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000355.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855844.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.

Price & Availability↑

CloneID	OHu83164
Clone ID Related Accession (Same CDS sequence)	XM_017000355.2 , XM_017000355.1
Accession Version	XM_017000355.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGCCCTG GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT 
GGAGACGGGA ACCTGCGTGT AGCAGTCGCT GGTCAGCGGT GGACCTTCAG CCCCTCCTGC 
CTGGTGGCCT ACCGGCCCGA GGAGGATGCC AACCTGGACG TGGCCGAGCG CGCCCGGGAG 
AACAAAAGCT CACTGAGCGT GGCCCTGGAC AAGCTTCGGG CCCAGAAGAG TGACCCAGAG 
CACCCGGGAA GGCTGGTGGT GGAGGTGGCG CTGGGTAACG CAGCCCGGGC TCTGGACCTG 
CTGCGGAGGC GCCCAGAGCA GGTGGACACC AAGAACCAAG GCAGGACCGC TCTGCAAGTG 
GCTGCCTACC TGGGCCAGGT GGAGTTGATA CGGCTGCTGC TACAAGCCAG GGCGGGCGTG 
GACCTGCCGG ACGACGAGGG CAACACGGCA CTGCACTACG CGGCCCTGGG GAACCAGCCC 
GAGGCCACCA GGGTGCTCCT GAGTGCTGGG TGCCGGGCGG ACGCCATCAA CAGCACCCAG 
AGCACAGCAC TGCACGTGGC CGTGCAGAGG GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT 
GAGCGCGGCT GTGACGTCAA CCTGCCCGAC GCCCACTCGG ACACGCCCCT GCACTCCGCC 
ATCTCGGCGG GCACTGGAGC CAGCGGCATT GTCGAGGTCC TCACGGAGGT GCCAAACATC 
GATGTTACCG CCACCAACAG CCAGGGTTTC ACCCTGCTGC ACCATGCCTC CCTCAAGGGT 
CACGCGCTAG CTGTGAGAAA GATTCTGGCT CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG 
GAGGACGGCT TCACGGCGCT GCATCTGGCT GCCCTCAACA ACCACCGCGA GGTGGCCCAG 
ATCCTCATCC GGGAGGGCCG CTGTGACGTG AACGTGCGCA ACCGGAAGCT GCAGTCCCCG 
CTGCATCTCG CCGTGCAACA GGCCCACGTG GGGCTGGTGC CGCTACTGGT GGACGCTGGG 
TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG GACACAGCCC TGCACGTGGC GCTGCAGCGT 
CATCAGCTGC TGCCCCTGGT GGCTGATGGG GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG 
CTGTCCAGGA CATGGGGCGC CGGCTGCTGG GGCTGCCAGG TGCCAGGAGA CGCCTCCCTC 
GGGCCTGCCC CGGCGCCCGC CCTCACCGGC GTCTGTCCTG CCGCCCAGCT ACAGGCCTCG 
GGCCTCCCCG GCAGCGCGGA GCTGACGGTG GGCGCGGCGG TCGCCTGCTT CCTGGCGCTG 
GAGGGCGCCG ACGTGAGCTA CACCAACCAC CGCGGTCGGA GCCCGCTGGA CCTGGCCGCC 
GAGGGTCGCG TGCTCAAGGC CCTTCAGGGC TGCGCCCAGC GCTTCCGGGA GCGGCAGGCG 
GGCGGGGGCG CGGCCCCGGG CCCCAGGCAA ACGCTCGGGA CCCCCAACAC CGTGACGAAC 
CTGCACGTGG GCGCCGCGCC GGGGCCCGAG GCCGCTGAGT GCCTGGTGTG CTCCGAGCTG 
GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACCG TGTGTGAGGG TGAGTGGGGG 
GCCCCGGGGT GGGGAGGCCC GGCTAGTAGG GCCGCAGCCA ACCGCGCTCT CCTCTTCGCA 
GAGTGCGCGC GCAGGATGAA GAAGTGCATC AGGTGCCAGG TGGTCGTCAG CAAGAAACTG 
CGCCCAGACG GCTCTGAGGT GGCGAGCGCC GCCCCCGCCC CCGGCCCGCC GCGCCAGCTG 
GTGGAGGAGC TGCAGAGCCG CTACCGGCAG ATGGAGGAAC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCACG GCGCATGCGC CCCCTGCGGC 
TCCGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT CCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855844.1
CDS	1726..5451
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000355.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000355.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGCCCTG GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT 
GGAGACGGGA ACCTGCGTGT AGCAGTCGCT GGTCAGCGGT GGACCTTCAG CCCCTCCTGC 
CTGGTGGCCT ACCGGCCCGA GGAGGATGCC AACCTGGACG TGGCCGAGCG CGCCCGGGAG 
AACAAAAGCT CACTGAGCGT GGCCCTGGAC AAGCTTCGGG CCCAGAAGAG TGACCCAGAG 
CACCCGGGAA GGCTGGTGGT GGAGGTGGCG CTGGGTAACG CAGCCCGGGC TCTGGACCTG 
CTGCGGAGGC GCCCAGAGCA GGTGGACACC AAGAACCAAG GCAGGACCGC TCTGCAAGTG 
GCTGCCTACC TGGGCCAGGT GGAGTTGATA CGGCTGCTGC TACAAGCCAG GGCGGGCGTG 
GACCTGCCGG ACGACGAGGG CAACACGGCA CTGCACTACG CGGCCCTGGG GAACCAGCCC 
GAGGCCACCA GGGTGCTCCT GAGTGCTGGG TGCCGGGCGG ACGCCATCAA CAGCACCCAG 
AGCACAGCAC TGCACGTGGC CGTGCAGAGG GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT 
GAGCGCGGCT GTGACGTCAA CCTGCCCGAC GCCCACTCGG ACACGCCCCT GCACTCCGCC 
ATCTCGGCGG GCACTGGAGC CAGCGGCATT GTCGAGGTCC TCACGGAGGT GCCAAACATC 
GATGTTACCG CCACCAACAG CCAGGGTTTC ACCCTGCTGC ACCATGCCTC CCTCAAGGGT 
CACGCGCTAG CTGTGAGAAA GATTCTGGCT CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG 
GAGGACGGCT TCACGGCGCT GCATCTGGCT GCCCTCAACA ACCACCGCGA GGTGGCCCAG 
ATCCTCATCC GGGAGGGCCG CTGTGACGTG AACGTGCGCA ACCGGAAGCT GCAGTCCCCG 
CTGCATCTCG CCGTGCAACA GGCCCACGTG GGGCTGGTGC CGCTACTGGT GGACGCTGGG 
TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG GACACAGCCC TGCACGTGGC GCTGCAGCGT 
CATCAGCTGC TGCCCCTGGT GGCTGATGGG GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG 
CTGTCCAGGA CATGGGGCGC CGGCTGCTGG GGCTGCCAGG TGCCAGGAGA CGCCTCCCTC 
GGGCCTGCCC CGGCGCCCGC CCTCACCGGC GTCTGTCCTG CCGCCCAGCT ACAGGCCTCG 
GGCCTCCCCG GCAGCGCGGA GCTGACGGTG GGCGCGGCGG TCGCCTGCTT CCTGGCGCTG 
GAGGGCGCCG ACGTGAGCTA CACCAACCAC CGCGGTCGGA GCCCGCTGGA CCTGGCCGCC 
GAGGGTCGCG TGCTCAAGGC CCTTCAGGGC TGCGCCCAGC GCTTCCGGGA GCGGCAGGCG 
GGCGGGGGCG CGGCCCCGGG CCCCAGGCAA ACGCTCGGGA CCCCCAACAC CGTGACGAAC 
CTGCACGTGG GCGCCGCGCC GGGGCCCGAG GCCGCTGAGT GCCTGGTGTG CTCCGAGCTG 
GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACCG TGTGTGAGGG TGAGTGGGGG 
GCCCCGGGGT GGGGAGGCCC GGCTAGTAGG GCCGCAGCCA ACCGCGCTCT CCTCTTCGCA 
GAGTGCGCGC GCAGGATGAA GAAGTGCATC AGGTGCCAGG TGGTCGTCAG CAAGAAACTG 
CGCCCAGACG GCTCTGAGGT GGCGAGCGCC GCCCCCGCCC CCGGCCCGCC GCGCCAGCTG 
GTGGAGGAGC TGCAGAGCCG CTACCGGCAG ATGGAGGAAC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCACG GCGCATGCGC CCCCTGCGGC 
TCCGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT CCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000355.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855844.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.

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CloneID	OHu83164
Clone ID Related Accession (Same CDS sequence)	XM_017000355.2 , XM_017000355.1
Accession Version	XM_017000355.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGCCCTG GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT 
GGAGACGGGA ACCTGCGTGT AGCAGTCGCT GGTCAGCGGT GGACCTTCAG CCCCTCCTGC 
CTGGTGGCCT ACCGGCCCGA GGAGGATGCC AACCTGGACG TGGCCGAGCG CGCCCGGGAG 
AACAAAAGCT CACTGAGCGT GGCCCTGGAC AAGCTTCGGG CCCAGAAGAG TGACCCAGAG 
CACCCGGGAA GGCTGGTGGT GGAGGTGGCG CTGGGTAACG CAGCCCGGGC TCTGGACCTG 
CTGCGGAGGC GCCCAGAGCA GGTGGACACC AAGAACCAAG GCAGGACCGC TCTGCAAGTG 
GCTGCCTACC TGGGCCAGGT GGAGTTGATA CGGCTGCTGC TACAAGCCAG GGCGGGCGTG 
GACCTGCCGG ACGACGAGGG CAACACGGCA CTGCACTACG CGGCCCTGGG GAACCAGCCC 
GAGGCCACCA GGGTGCTCCT GAGTGCTGGG TGCCGGGCGG ACGCCATCAA CAGCACCCAG 
AGCACAGCAC TGCACGTGGC CGTGCAGAGG GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT 
GAGCGCGGCT GTGACGTCAA CCTGCCCGAC GCCCACTCGG ACACGCCCCT GCACTCCGCC 
ATCTCGGCGG GCACTGGAGC CAGCGGCATT GTCGAGGTCC TCACGGAGGT GCCAAACATC 
GATGTTACCG CCACCAACAG CCAGGGTTTC ACCCTGCTGC ACCATGCCTC CCTCAAGGGT 
CACGCGCTAG CTGTGAGAAA GATTCTGGCT CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG 
GAGGACGGCT TCACGGCGCT GCATCTGGCT GCCCTCAACA ACCACCGCGA GGTGGCCCAG 
ATCCTCATCC GGGAGGGCCG CTGTGACGTG AACGTGCGCA ACCGGAAGCT GCAGTCCCCG 
CTGCATCTCG CCGTGCAACA GGCCCACGTG GGGCTGGTGC CGCTACTGGT GGACGCTGGG 
TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG GACACAGCCC TGCACGTGGC GCTGCAGCGT 
CATCAGCTGC TGCCCCTGGT GGCTGATGGG GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG 
CTGTCCAGGA CATGGGGCGC CGGCTGCTGG GGCTGCCAGG TGCCAGGAGA CGCCTCCCTC 
GGGCCTGCCC CGGCGCCCGC CCTCACCGGC GTCTGTCCTG CCGCCCAGCT ACAGGCCTCG 
GGCCTCCCCG GCAGCGCGGA GCTGACGGTG GGCGCGGCGG TCGCCTGCTT CCTGGCGCTG 
GAGGGCGCCG ACGTGAGCTA CACCAACCAC CGCGGTCGGA GCCCGCTGGA CCTGGCCGCC 
GAGGGTCGCG TGCTCAAGGC CCTTCAGGGC TGCGCCCAGC GCTTCCGGGA GCGGCAGGCG 
GGCGGGGGCG CGGCCCCGGG CCCCAGGCAA ACGCTCGGGA CCCCCAACAC CGTGACGAAC 
CTGCACGTGG GCGCCGCGCC GGGGCCCGAG GCCGCTGAGT GCCTGGTGTG CTCCGAGCTG 
GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACCG TGTGTGAGGG TGAGTGGGGG 
GCCCCGGGGT GGGGAGGCCC GGCTAGTAGG GCCGCAGCCA ACCGCGCTCT CCTCTTCGCA 
GAGTGCGCGC GCAGGATGAA GAAGTGCATC AGGTGCCAGG TGGTCGTCAG CAAGAAACTG 
CGCCCAGACG GCTCTGAGGT GGCGAGCGCC GCCCCCGCCC CCGGCCCGCC GCGCCAGCTG 
GTGGAGGAGC TGCAGAGCCG CTACCGGCAG ATGGAGGAAC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCACG GCGCATGCGC CCCCTGCGGC 
TCCGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT CCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855844.1
CDS	273..3998
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000355.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000355.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGTTTA TCGGACAGAC GGGCACCGTG 
CATCGTATCA CGGACCGCGG GGACGTGCGC GTGCAGTTCA ACCACGAGAC GCGCTGGACC 
TTCCACCCCG GGGCGCTCAC CAAGGCCCTG GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT 
GGAGACGGGA ACCTGCGTGT AGCAGTCGCT GGTCAGCGGT GGACCTTCAG CCCCTCCTGC 
CTGGTGGCCT ACCGGCCCGA GGAGGATGCC AACCTGGACG TGGCCGAGCG CGCCCGGGAG 
AACAAAAGCT CACTGAGCGT GGCCCTGGAC AAGCTTCGGG CCCAGAAGAG TGACCCAGAG 
CACCCGGGAA GGCTGGTGGT GGAGGTGGCG CTGGGTAACG CAGCCCGGGC TCTGGACCTG 
CTGCGGAGGC GCCCAGAGCA GGTGGACACC AAGAACCAAG GCAGGACCGC TCTGCAAGTG 
GCTGCCTACC TGGGCCAGGT GGAGTTGATA CGGCTGCTGC TACAAGCCAG GGCGGGCGTG 
GACCTGCCGG ACGACGAGGG CAACACGGCA CTGCACTACG CGGCCCTGGG GAACCAGCCC 
GAGGCCACCA GGGTGCTCCT GAGTGCTGGG TGCCGGGCGG ACGCCATCAA CAGCACCCAG 
AGCACAGCAC TGCACGTGGC CGTGCAGAGG GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT 
GAGCGCGGCT GTGACGTCAA CCTGCCCGAC GCCCACTCGG ACACGCCCCT GCACTCCGCC 
ATCTCGGCGG GCACTGGAGC CAGCGGCATT GTCGAGGTCC TCACGGAGGT GCCAAACATC 
GATGTTACCG CCACCAACAG CCAGGGTTTC ACCCTGCTGC ACCATGCCTC CCTCAAGGGT 
CACGCGCTAG CTGTGAGAAA GATTCTGGCT CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG 
GAGGACGGCT TCACGGCGCT GCATCTGGCT GCCCTCAACA ACCACCGCGA GGTGGCCCAG 
ATCCTCATCC GGGAGGGCCG CTGTGACGTG AACGTGCGCA ACCGGAAGCT GCAGTCCCCG 
CTGCATCTCG CCGTGCAACA GGCCCACGTG GGGCTGGTGC CGCTACTGGT GGACGCTGGG 
TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG GACACAGCCC TGCACGTGGC GCTGCAGCGT 
CATCAGCTGC TGCCCCTGGT GGCTGATGGG GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG 
CTGTCCAGGA CATGGGGCGC CGGCTGCTGG GGCTGCCAGG TGCCAGGAGA CGCCTCCCTC 
GGGCCTGCCC CGGCGCCCGC CCTCACCGGC GTCTGTCCTG CCGCCCAGCT ACAGGCCTCG 
GGCCTCCCCG GCAGCGCGGA GCTGACGGTG GGCGCGGCGG TCGCCTGCTT CCTGGCGCTG 
GAGGGCGCCG ACGTGAGCTA CACCAACCAC CGCGGTCGGA GCCCGCTGGA CCTGGCCGCC 
GAGGGTCGCG TGCTCAAGGC CCTTCAGGGC TGCGCCCAGC GCTTCCGGGA GCGGCAGGCG 
GGCGGGGGCG CGGCCCCGGG CCCCAGGCAA ACGCTCGGGA CCCCCAACAC CGTGACGAAC 
CTGCACGTGG GCGCCGCGCC GGGGCCCGAG GCCGCTGAGT GCCTGGTGTG CTCCGAGCTG 
GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG CACCGCACCG TGTGTGAGGG TGAGTGGGGG 
GCCCCGGGGT GGGGAGGCCC GGCTAGTAGG GCCGCAGCCA ACCGCGCTCT CCTCTTCGCA 
GAGTGCGCGC GCAGGATGAA GAAGTGCATC AGGTGCCAGG TGGTCGTCAG CAAGAAACTG 
CGCCCAGACG GCTCTGAGGT GGCGAGCGCC GCCCCCGCCC CCGGCCCGCC GCGCCAGCTG 
GTGGAGGAGC TGCAGAGCCG CTACCGGCAG ATGGAGGAAC GCATCACCTG CCCCATCTGC 
ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCACG GCGCATGCGC CCCCTGCGGC 
TCCGCGCTCA GCGCCTGCCC CATCTGCCGC CAGCCCATCC GCGACCGCAT CCAGATCTTC 
GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000356.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855845.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu83165
Clone ID Related Accession (Same CDS sequence)	XM_017000356.1 , XM_017000356.2
Accession Version	XM_017000356.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGCACC ACTCCTTCTG GGTGGGCGAC 
GTGGTCCGGG TCATCGGCGA CCTTGACACA GTGAAGCGGC TGCAGGCTGG GCATGGCGAG 
TGGACGGACG ACATGGCCCC TGCCCTGGGC CGCGTCGGGA AGGTGGTGAA AGTGTTTGGA 
GACGGGAACC TGCGTGTAGC AGTCGCTGGT CAGCGGTGGA CCTTCAGCCC CTCCTGCCTG 
GTGGCCTACC GGCCCGAGGA GGATGCCAAC CTGGACGTGG CCGAGCGCGC CCGGGAGAAC 
AAAAGCTCAC TGAGCGTGGC CCTGGACAAG CTTCGGGCCC AGAAGAGTGA CCCAGAGCAC 
CCGGGAAGGC TGGTGGTGGA GGTGGCGCTG GGTAACGCAG CCCGGGCTCT GGACCTGCTG 
CGGAGGCGCC CAGAGCAGGT GGACACCAAG AACCAAGGCA GGACCGCTCT GCAAGTGGCT 
GCCTACCTGG GCCAGGTGGA GTTGATACGG CTGCTGCTAC AAGCCAGGGC GGGCGTGGAC 
CTGCCGGACG ACGAGGGCAA CACGGCACTG CACTACGCGG CCCTGGGGAA CCAGCCCGAG 
GCCACCAGGG TGCTCCTGAG TGCTGGGTGC CGGGCGGACG CCATCAACAG CACCCAGAGC 
ACAGCACTGC ACGTGGCCGT GCAGAGGGGC TTCCTGGAGG TGGTGCGGGC CCTGTGTGAG 
CGCGGCTGTG ACGTCAACCT GCCCGACGCC CACTCGGACA CGCCCCTGCA CTCCGCCATC 
TCGGCGGGCA CTGGAGCCAG CGGCATTGTC GAGGTCCTCA CGGAGGTGCC AAACATCGAT 
GTTACCGCCA CCAACAGCCA GGGTTTCACC CTGCTGCACC ATGCCTCCCT CAAGGGTCAC 
GCGCTAGCTG TGAGAAAGAT TCTGGCTCGG GCGCGGCAGC TGGTGGACGC CAAGAAGGAG 
GACGGCTTCA CGGCGCTGCA TCTGGCTGCC CTCAACAACC ACCGCGAGGT GGCCCAGATC 
CTCATCCGGG AGGGCCGCTG TGACGTGAAC GTGCGCAACC GGAAGCTGCA GTCCCCGCTG 
CATCTCGCCG TGCAACAGGC CCACGTGGGG CTGGTGCCGC TACTGGTGGA CGCTGGGTGC 
AGTGTCAACG CCGAGGACGA GGAGGGGGAC ACAGCCCTGC ACGTGGCGCT GCAGCGTCAT 
CAGCTGCTGC CCCTGGTGGC TGATGGGGCC GGGGGGGACC CAGGGCCCTT GCAGCTGCTG 
TCCAGGACAT GGGGCGCCGG CTGCTGGGGC TGCCAGGTGC CAGGAGACGC CTCCCTCGGG 
CCTGCCCCGG CGCCCGCCCT CACCGGCGTC TGTCCTGCCG CCCAGCTACA GGCCTCGGGC 
CTCCCCGGCA GCGCGGAGCT GACGGTGGGC GCGGCGGTCG CCTGCTTCCT GGCGCTGGAG 
GGCGCCGACG TGAGCTACAC CAACCACCGC GGTCGGAGCC CGCTGGACCT GGCCGCCGAG 
GGTCGCGTGC TCAAGGCCCT TCAGGGCTGC GCCCAGCGCT TCCGGGAGCG GCAGGCGGGC 
GGGGGCGCGG CCCCGGGCCC CAGGCAAACG CTCGGGACCC CCAACACCGT GACGAACCTG 
CACGTGGGCG CCGCGCCGGG GCCCGAGGCC GCTGAGTGCC TGGTGTGCTC CGAGCTGGCG 
CTGCTGGTGC TGTTCTCGCC GTGCCAGCAC CGCACCGTGT GTGAGGGTGA GTGGGGGGCC 
CCGGGGTGGG GAGGCCCGGC TAGTAGGGCC GCAGCCAACC GCGCTCTCCT CTTCGCAGAG 
TGCGCGCGCA GGATGAAGAA GTGCATCAGG TGCCAGGTGG TCGTCAGCAA GAAACTGCGC 
CCAGACGGCT CTGAGGTGGC GAGCGCCGCC CCCGCCCCCG GCCCGCCGCG CCAGCTGGTG 
GAGGAGCTGC AGAGCCGCTA CCGGCAGATG GAGGAACGCA TCACCTGCCC CATCTGCATC 
GACAGCCACA TCCGCCTCGT GTTCCAGTGC GGCCACGGCG CATGCGCCCC CTGCGGCTCC 
GCGCTCAGCG CCTGCCCCAT CTGCCGCCAG CCCATCCGCG ACCGCATCCA GATCTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855845.1
CDS	273..3995
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000356.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000356.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGCACC ACTCCTTCTG GGTGGGCGAC 
GTGGTCCGGG TCATCGGCGA CCTTGACACA GTGAAGCGGC TGCAGGCTGG GCATGGCGAG 
TGGACGGACG ACATGGCCCC TGCCCTGGGC CGCGTCGGGA AGGTGGTGAA AGTGTTTGGA 
GACGGGAACC TGCGTGTAGC AGTCGCTGGT CAGCGGTGGA CCTTCAGCCC CTCCTGCCTG 
GTGGCCTACC GGCCCGAGGA GGATGCCAAC CTGGACGTGG CCGAGCGCGC CCGGGAGAAC 
AAAAGCTCAC TGAGCGTGGC CCTGGACAAG CTTCGGGCCC AGAAGAGTGA CCCAGAGCAC 
CCGGGAAGGC TGGTGGTGGA GGTGGCGCTG GGTAACGCAG CCCGGGCTCT GGACCTGCTG 
CGGAGGCGCC CAGAGCAGGT GGACACCAAG AACCAAGGCA GGACCGCTCT GCAAGTGGCT 
GCCTACCTGG GCCAGGTGGA GTTGATACGG CTGCTGCTAC AAGCCAGGGC GGGCGTGGAC 
CTGCCGGACG ACGAGGGCAA CACGGCACTG CACTACGCGG CCCTGGGGAA CCAGCCCGAG 
GCCACCAGGG TGCTCCTGAG TGCTGGGTGC CGGGCGGACG CCATCAACAG CACCCAGAGC 
ACAGCACTGC ACGTGGCCGT GCAGAGGGGC TTCCTGGAGG TGGTGCGGGC CCTGTGTGAG 
CGCGGCTGTG ACGTCAACCT GCCCGACGCC CACTCGGACA CGCCCCTGCA CTCCGCCATC 
TCGGCGGGCA CTGGAGCCAG CGGCATTGTC GAGGTCCTCA CGGAGGTGCC AAACATCGAT 
GTTACCGCCA CCAACAGCCA GGGTTTCACC CTGCTGCACC ATGCCTCCCT CAAGGGTCAC 
GCGCTAGCTG TGAGAAAGAT TCTGGCTCGG GCGCGGCAGC TGGTGGACGC CAAGAAGGAG 
GACGGCTTCA CGGCGCTGCA TCTGGCTGCC CTCAACAACC ACCGCGAGGT GGCCCAGATC 
CTCATCCGGG AGGGCCGCTG TGACGTGAAC GTGCGCAACC GGAAGCTGCA GTCCCCGCTG 
CATCTCGCCG TGCAACAGGC CCACGTGGGG CTGGTGCCGC TACTGGTGGA CGCTGGGTGC 
AGTGTCAACG CCGAGGACGA GGAGGGGGAC ACAGCCCTGC ACGTGGCGCT GCAGCGTCAT 
CAGCTGCTGC CCCTGGTGGC TGATGGGGCC GGGGGGGACC CAGGGCCCTT GCAGCTGCTG 
TCCAGGACAT GGGGCGCCGG CTGCTGGGGC TGCCAGGTGC CAGGAGACGC CTCCCTCGGG 
CCTGCCCCGG CGCCCGCCCT CACCGGCGTC TGTCCTGCCG CCCAGCTACA GGCCTCGGGC 
CTCCCCGGCA GCGCGGAGCT GACGGTGGGC GCGGCGGTCG CCTGCTTCCT GGCGCTGGAG 
GGCGCCGACG TGAGCTACAC CAACCACCGC GGTCGGAGCC CGCTGGACCT GGCCGCCGAG 
GGTCGCGTGC TCAAGGCCCT TCAGGGCTGC GCCCAGCGCT TCCGGGAGCG GCAGGCGGGC 
GGGGGCGCGG CCCCGGGCCC CAGGCAAACG CTCGGGACCC CCAACACCGT GACGAACCTG 
CACGTGGGCG CCGCGCCGGG GCCCGAGGCC GCTGAGTGCC TGGTGTGCTC CGAGCTGGCG 
CTGCTGGTGC TGTTCTCGCC GTGCCAGCAC CGCACCGTGT GTGAGGGTGA GTGGGGGGCC 
CCGGGGTGGG GAGGCCCGGC TAGTAGGGCC GCAGCCAACC GCGCTCTCCT CTTCGCAGAG 
TGCGCGCGCA GGATGAAGAA GTGCATCAGG TGCCAGGTGG TCGTCAGCAA GAAACTGCGC 
CCAGACGGCT CTGAGGTGGC GAGCGCCGCC CCCGCCCCCG GCCCGCCGCG CCAGCTGGTG 
GAGGAGCTGC AGAGCCGCTA CCGGCAGATG GAGGAACGCA TCACCTGCCC CATCTGCATC 
GACAGCCACA TCCGCCTCGT GTTCCAGTGC GGCCACGGCG CATGCGCCCC CTGCGGCTCC 
GCGCTCAGCG CCTGCCCCAT CTGCCGCCAG CCCATCCGCG ACCGCATCCA GATCTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000356.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855845.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu83165
Clone ID Related Accession (Same CDS sequence)	XM_017000356.1 , XM_017000356.2
Accession Version	XM_017000356.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGCACC ACTCCTTCTG GGTGGGCGAC 
GTGGTCCGGG TCATCGGCGA CCTTGACACA GTGAAGCGGC TGCAGGCTGG GCATGGCGAG 
TGGACGGACG ACATGGCCCC TGCCCTGGGC CGCGTCGGGA AGGTGGTGAA AGTGTTTGGA 
GACGGGAACC TGCGTGTAGC AGTCGCTGGT CAGCGGTGGA CCTTCAGCCC CTCCTGCCTG 
GTGGCCTACC GGCCCGAGGA GGATGCCAAC CTGGACGTGG CCGAGCGCGC CCGGGAGAAC 
AAAAGCTCAC TGAGCGTGGC CCTGGACAAG CTTCGGGCCC AGAAGAGTGA CCCAGAGCAC 
CCGGGAAGGC TGGTGGTGGA GGTGGCGCTG GGTAACGCAG CCCGGGCTCT GGACCTGCTG 
CGGAGGCGCC CAGAGCAGGT GGACACCAAG AACCAAGGCA GGACCGCTCT GCAAGTGGCT 
GCCTACCTGG GCCAGGTGGA GTTGATACGG CTGCTGCTAC AAGCCAGGGC GGGCGTGGAC 
CTGCCGGACG ACGAGGGCAA CACGGCACTG CACTACGCGG CCCTGGGGAA CCAGCCCGAG 
GCCACCAGGG TGCTCCTGAG TGCTGGGTGC CGGGCGGACG CCATCAACAG CACCCAGAGC 
ACAGCACTGC ACGTGGCCGT GCAGAGGGGC TTCCTGGAGG TGGTGCGGGC CCTGTGTGAG 
CGCGGCTGTG ACGTCAACCT GCCCGACGCC CACTCGGACA CGCCCCTGCA CTCCGCCATC 
TCGGCGGGCA CTGGAGCCAG CGGCATTGTC GAGGTCCTCA CGGAGGTGCC AAACATCGAT 
GTTACCGCCA CCAACAGCCA GGGTTTCACC CTGCTGCACC ATGCCTCCCT CAAGGGTCAC 
GCGCTAGCTG TGAGAAAGAT TCTGGCTCGG GCGCGGCAGC TGGTGGACGC CAAGAAGGAG 
GACGGCTTCA CGGCGCTGCA TCTGGCTGCC CTCAACAACC ACCGCGAGGT GGCCCAGATC 
CTCATCCGGG AGGGCCGCTG TGACGTGAAC GTGCGCAACC GGAAGCTGCA GTCCCCGCTG 
CATCTCGCCG TGCAACAGGC CCACGTGGGG CTGGTGCCGC TACTGGTGGA CGCTGGGTGC 
AGTGTCAACG CCGAGGACGA GGAGGGGGAC ACAGCCCTGC ACGTGGCGCT GCAGCGTCAT 
CAGCTGCTGC CCCTGGTGGC TGATGGGGCC GGGGGGGACC CAGGGCCCTT GCAGCTGCTG 
TCCAGGACAT GGGGCGCCGG CTGCTGGGGC TGCCAGGTGC CAGGAGACGC CTCCCTCGGG 
CCTGCCCCGG CGCCCGCCCT CACCGGCGTC TGTCCTGCCG CCCAGCTACA GGCCTCGGGC 
CTCCCCGGCA GCGCGGAGCT GACGGTGGGC GCGGCGGTCG CCTGCTTCCT GGCGCTGGAG 
GGCGCCGACG TGAGCTACAC CAACCACCGC GGTCGGAGCC CGCTGGACCT GGCCGCCGAG 
GGTCGCGTGC TCAAGGCCCT TCAGGGCTGC GCCCAGCGCT TCCGGGAGCG GCAGGCGGGC 
GGGGGCGCGG CCCCGGGCCC CAGGCAAACG CTCGGGACCC CCAACACCGT GACGAACCTG 
CACGTGGGCG CCGCGCCGGG GCCCGAGGCC GCTGAGTGCC TGGTGTGCTC CGAGCTGGCG 
CTGCTGGTGC TGTTCTCGCC GTGCCAGCAC CGCACCGTGT GTGAGGGTGA GTGGGGGGCC 
CCGGGGTGGG GAGGCCCGGC TAGTAGGGCC GCAGCCAACC GCGCTCTCCT CTTCGCAGAG 
TGCGCGCGCA GGATGAAGAA GTGCATCAGG TGCCAGGTGG TCGTCAGCAA GAAACTGCGC 
CCAGACGGCT CTGAGGTGGC GAGCGCCGCC CCCGCCCCCG GCCCGCCGCG CCAGCTGGTG 
GAGGAGCTGC AGAGCCGCTA CCGGCAGATG GAGGAACGCA TCACCTGCCC CATCTGCATC 
GACAGCCACA TCCGCCTCGT GTTCCAGTGC GGCCACGGCG CATGCGCCCC CTGCGGCTCC 
GCGCTCAGCG CCTGCCCCAT CTGCCGCCAG CCCATCCGCG ACCGCATCCA GATCTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855845.1
CDS	1726..5448
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000356.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000356.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGA 
GGGGAAGGGA AACCGGGCCG TGTGGTGGAC ATCCGTGGCT GGGATGTGGA GACAGGCCGG 
AGTGTGGCCA GCGTGACGTG GGCTGATGGT ACCACCAATG TGTACCGTGT GGGCCACAAG 
GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG GCAGCGGGCG GCTTCTACTA CAAGGACCAC 
CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC 
TTCCAGCACG GGGACAAGGT CAAGTGTCTG CTGGACACTG ATGTCCTGCG GGAGATGCAG 
GAAGGCCACG GCGGCTGGAA CCCCAGGATG GCGGAGCACC ACTCCTTCTG GGTGGGCGAC 
GTGGTCCGGG TCATCGGCGA CCTTGACACA GTGAAGCGGC TGCAGGCTGG GCATGGCGAG 
TGGACGGACG ACATGGCCCC TGCCCTGGGC CGCGTCGGGA AGGTGGTGAA AGTGTTTGGA 
GACGGGAACC TGCGTGTAGC AGTCGCTGGT CAGCGGTGGA CCTTCAGCCC CTCCTGCCTG 
GTGGCCTACC GGCCCGAGGA GGATGCCAAC CTGGACGTGG CCGAGCGCGC CCGGGAGAAC 
AAAAGCTCAC TGAGCGTGGC CCTGGACAAG CTTCGGGCCC AGAAGAGTGA CCCAGAGCAC 
CCGGGAAGGC TGGTGGTGGA GGTGGCGCTG GGTAACGCAG CCCGGGCTCT GGACCTGCTG 
CGGAGGCGCC CAGAGCAGGT GGACACCAAG AACCAAGGCA GGACCGCTCT GCAAGTGGCT 
GCCTACCTGG GCCAGGTGGA GTTGATACGG CTGCTGCTAC AAGCCAGGGC GGGCGTGGAC 
CTGCCGGACG ACGAGGGCAA CACGGCACTG CACTACGCGG CCCTGGGGAA CCAGCCCGAG 
GCCACCAGGG TGCTCCTGAG TGCTGGGTGC CGGGCGGACG CCATCAACAG CACCCAGAGC 
ACAGCACTGC ACGTGGCCGT GCAGAGGGGC TTCCTGGAGG TGGTGCGGGC CCTGTGTGAG 
CGCGGCTGTG ACGTCAACCT GCCCGACGCC CACTCGGACA CGCCCCTGCA CTCCGCCATC 
TCGGCGGGCA CTGGAGCCAG CGGCATTGTC GAGGTCCTCA CGGAGGTGCC AAACATCGAT 
GTTACCGCCA CCAACAGCCA GGGTTTCACC CTGCTGCACC ATGCCTCCCT CAAGGGTCAC 
GCGCTAGCTG TGAGAAAGAT TCTGGCTCGG GCGCGGCAGC TGGTGGACGC CAAGAAGGAG 
GACGGCTTCA CGGCGCTGCA TCTGGCTGCC CTCAACAACC ACCGCGAGGT GGCCCAGATC 
CTCATCCGGG AGGGCCGCTG TGACGTGAAC GTGCGCAACC GGAAGCTGCA GTCCCCGCTG 
CATCTCGCCG TGCAACAGGC CCACGTGGGG CTGGTGCCGC TACTGGTGGA CGCTGGGTGC 
AGTGTCAACG CCGAGGACGA GGAGGGGGAC ACAGCCCTGC ACGTGGCGCT GCAGCGTCAT 
CAGCTGCTGC CCCTGGTGGC TGATGGGGCC GGGGGGGACC CAGGGCCCTT GCAGCTGCTG 
TCCAGGACAT GGGGCGCCGG CTGCTGGGGC TGCCAGGTGC CAGGAGACGC CTCCCTCGGG 
CCTGCCCCGG CGCCCGCCCT CACCGGCGTC TGTCCTGCCG CCCAGCTACA GGCCTCGGGC 
CTCCCCGGCA GCGCGGAGCT GACGGTGGGC GCGGCGGTCG CCTGCTTCCT GGCGCTGGAG 
GGCGCCGACG TGAGCTACAC CAACCACCGC GGTCGGAGCC CGCTGGACCT GGCCGCCGAG 
GGTCGCGTGC TCAAGGCCCT TCAGGGCTGC GCCCAGCGCT TCCGGGAGCG GCAGGCGGGC 
GGGGGCGCGG CCCCGGGCCC CAGGCAAACG CTCGGGACCC CCAACACCGT GACGAACCTG 
CACGTGGGCG CCGCGCCGGG GCCCGAGGCC GCTGAGTGCC TGGTGTGCTC CGAGCTGGCG 
CTGCTGGTGC TGTTCTCGCC GTGCCAGCAC CGCACCGTGT GTGAGGGTGA GTGGGGGGCC 
CCGGGGTGGG GAGGCCCGGC TAGTAGGGCC GCAGCCAACC GCGCTCTCCT CTTCGCAGAG 
TGCGCGCGCA GGATGAAGAA GTGCATCAGG TGCCAGGTGG TCGTCAGCAA GAAACTGCGC 
CCAGACGGCT CTGAGGTGGC GAGCGCCGCC CCCGCCCCCG GCCCGCCGCG CCAGCTGGTG 
GAGGAGCTGC AGAGCCGCTA CCGGCAGATG GAGGAACGCA TCACCTGCCC CATCTGCATC 
GACAGCCACA TCCGCCTCGT GTTCCAGTGC GGCCACGGCG CATGCGCCCC CTGCGGCTCC 
GCGCTCAGCG CCTGCCCCAT CTGCCGCCAG CCCATCCGCG ACCGCATCCA GATCTTCGTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000357.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855846.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA.

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CloneID	OHu83166
Clone ID Related Accession (Same CDS sequence)	XM_017000357.1 , XM_017000357.2
Accession Version	XM_017000357.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGC 
AAGCCGGCGG AGCTGCAGCG CAGGGTGAGT GCTGACAGCC AGCCCTTCCA GCACGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACTGATGTC CTGCGGGAGA TGCAGGAAGG CCACGGCGGC 
TGGAACCCCA GGATGGCGGA GTTTATCGGA CAGACGGGCA CCGTGCATCG TATCACGGAC 
CGCGGGGACG TGCGCGTGCA GTTCAACCAC GAGACGCGCT GGACCTTCCA CCCCGGGGCG 
CTCACCAAGG ACACCAGGAA GGCAGGACAG CTTCGTGGGC GGGAGGGAGG CGGCTGGGCT 
AAGATGCTCC TGCACCACTC CTTCTGGGTG GGCGACGTGG TCCGGGTCAT CGGCGACCTT 
GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CGGACGACAT GGCCCCTGCC 
CTGGGCCGCG TCGGGAAGGT GGTGAAAGTG TTTGGAGACG GGAACCTGCG TGTAGCAGTC 
GCTGGTCAGC GGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC CGAGGAGGAT 
GCCAACCTGG ACGTGGCCGA GCGCGCCCGG GAGAACAAAA GCTCACTGAG CGTGGCCCTG 
GACAAGCTTC GGGCCCAGAA GAGTGACCCA GAGCACCCGG GAAGGCTGGT GGTGGAGGTG 
GCGCTGGGTA ACGCAGCCCG GGCTCTGGAC CTGCTGCGGA GGCGCCCAGA GCAGGTGGAC 
ACCAAGAACC AAGGCAGGAC CGCTCTGCAA GTGGCTGCCT ACCTGGGCCA GGTGGAGTTG 
ATACGGCTGC TGCTACAAGC CAGGGCGGGC GTGGACCTGC CGGACGACGA GGGCAACACG 
GCACTGCACT ACGCGGCCCT GGGGAACCAG CCCGAGGCCA CCAGGGTGCT CCTGAGTGCT 
GGGTGCCGGG CGGACGCCAT CAACAGCACC CAGAGCACAG CACTGCACGT GGCCGTGCAG 
AGGGGCTTCC TGGAGGTGGT GCGGGCCCTG TGTGAGCGCG GCTGTGACGT CAACCTGCCC 
GACGCCCACT CGGACACGCC CCTGCACTCC GCCATCTCGG CGGGCACTGG AGCCAGCGGC 
ATTGTCGAGG TCCTCACGGA GGTGCCAAAC ATCGATGTTA CCGCCACCAA CAGCCAGGGT 
TTCACCCTGC TGCACCATGC CTCCCTCAAG GGTCACGCGC TAGCTGTGAG AAAGATTCTG 
GCTCGGGCGC GGCAGCTGGT GGACGCCAAG AAGGAGGACG GCTTCACGGC GCTGCATCTG 
GCTGCCCTCA ACAACCACCG CGAGGTGGCC CAGATCCTCA TCCGGGAGGG CCGCTGTGAC 
GTGAACGTGC GCAACCGGAA GCTGCAGTCC CCGCTGCATC TCGCCGTGCA ACAGGCCCAC 
GTGGGGCTGG TGCCGCTACT GGTGGACGCT GGGTGCAGTG TCAACGCCGA GGACGAGGAG 
GGGGACACAG CCCTGCACGT GGCGCTGCAG CGTCATCAGC TGCTGCCCCT GGTGGCTGAT 
GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGACATGGGG CGCCGGCTGC 
TGGGGCTGCC AGGTGCCAGG AGACGCCTCC CTCGGGCCTG CCCCGGCGCC CGCCCTCACC 
GGCGTCTGTC CTGCCGCCCA GCTACAGGCC TCGGGCCTCC CCGGCAGCGC GGAGCTGACG 
GTGGGCGCGG CGGTCGCCTG CTTCCTGGCG CTGGAGGGCG CCGACGTGAG CTACACCAAC 
CACCGCGGTC GGAGCCCGCT GGACCTGGCC GCCGAGGGTC GCGTGCTCAA GGCCCTTCAG 
GGCTGCGCCC AGCGCTTCCG GGAGCGGCAG GCGGGCGGGG GCGCGGCCCC GGGCCCCAGG 
CAAACGCTCG GGACCCCCAA CACCGTGACG AACCTGCACG TGGGCGCCGC GCCGGGGCCC 
GAGGCCGCTG AGTGCCTGGT GTGCTCCGAG CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC 
CAGCACCGCA CCGTGTGTGA GGGTGAGTGG GGGGCCCCGG GGTGGGGAGG CCCGGCTAGT 
AGGGCCGCAG CCAACCGCGC TCTCCTCTTC GCAGAGTGCG CGCGCAGGAT GAAGAAGTGC 
ATCAGGTGCC AGGTGGTCGT CAGCAAGAAA CTGCGCCCAG ACGGCTCTGA GGTGGCGAGC 
GCCGCCCCCG CCCCCGGCCC GCCGCGCCAG CTGGTGGAGG AGCTGCAGAG CCGCTACCGG 
CAGATGGAGG AACGCATCAC CTGCCCCATC TGCATCGACA GCCACATCCG CCTCGTGTTC 
CAGTGCGGCC ACGGCGCATG CGCCCCCTGC GGCTCCGCGC TCAGCGCCTG CCCCATCTGC 
CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855846.1
CDS	273..3971
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000357.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000357.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGC 
AAGCCGGCGG AGCTGCAGCG CAGGGTGAGT GCTGACAGCC AGCCCTTCCA GCACGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACTGATGTC CTGCGGGAGA TGCAGGAAGG CCACGGCGGC 
TGGAACCCCA GGATGGCGGA GTTTATCGGA CAGACGGGCA CCGTGCATCG TATCACGGAC 
CGCGGGGACG TGCGCGTGCA GTTCAACCAC GAGACGCGCT GGACCTTCCA CCCCGGGGCG 
CTCACCAAGG ACACCAGGAA GGCAGGACAG CTTCGTGGGC GGGAGGGAGG CGGCTGGGCT 
AAGATGCTCC TGCACCACTC CTTCTGGGTG GGCGACGTGG TCCGGGTCAT CGGCGACCTT 
GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CGGACGACAT GGCCCCTGCC 
CTGGGCCGCG TCGGGAAGGT GGTGAAAGTG TTTGGAGACG GGAACCTGCG TGTAGCAGTC 
GCTGGTCAGC GGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC CGAGGAGGAT 
GCCAACCTGG ACGTGGCCGA GCGCGCCCGG GAGAACAAAA GCTCACTGAG CGTGGCCCTG 
GACAAGCTTC GGGCCCAGAA GAGTGACCCA GAGCACCCGG GAAGGCTGGT GGTGGAGGTG 
GCGCTGGGTA ACGCAGCCCG GGCTCTGGAC CTGCTGCGGA GGCGCCCAGA GCAGGTGGAC 
ACCAAGAACC AAGGCAGGAC CGCTCTGCAA GTGGCTGCCT ACCTGGGCCA GGTGGAGTTG 
ATACGGCTGC TGCTACAAGC CAGGGCGGGC GTGGACCTGC CGGACGACGA GGGCAACACG 
GCACTGCACT ACGCGGCCCT GGGGAACCAG CCCGAGGCCA CCAGGGTGCT CCTGAGTGCT 
GGGTGCCGGG CGGACGCCAT CAACAGCACC CAGAGCACAG CACTGCACGT GGCCGTGCAG 
AGGGGCTTCC TGGAGGTGGT GCGGGCCCTG TGTGAGCGCG GCTGTGACGT CAACCTGCCC 
GACGCCCACT CGGACACGCC CCTGCACTCC GCCATCTCGG CGGGCACTGG AGCCAGCGGC 
ATTGTCGAGG TCCTCACGGA GGTGCCAAAC ATCGATGTTA CCGCCACCAA CAGCCAGGGT 
TTCACCCTGC TGCACCATGC CTCCCTCAAG GGTCACGCGC TAGCTGTGAG AAAGATTCTG 
GCTCGGGCGC GGCAGCTGGT GGACGCCAAG AAGGAGGACG GCTTCACGGC GCTGCATCTG 
GCTGCCCTCA ACAACCACCG CGAGGTGGCC CAGATCCTCA TCCGGGAGGG CCGCTGTGAC 
GTGAACGTGC GCAACCGGAA GCTGCAGTCC CCGCTGCATC TCGCCGTGCA ACAGGCCCAC 
GTGGGGCTGG TGCCGCTACT GGTGGACGCT GGGTGCAGTG TCAACGCCGA GGACGAGGAG 
GGGGACACAG CCCTGCACGT GGCGCTGCAG CGTCATCAGC TGCTGCCCCT GGTGGCTGAT 
GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGACATGGGG CGCCGGCTGC 
TGGGGCTGCC AGGTGCCAGG AGACGCCTCC CTCGGGCCTG CCCCGGCGCC CGCCCTCACC 
GGCGTCTGTC CTGCCGCCCA GCTACAGGCC TCGGGCCTCC CCGGCAGCGC GGAGCTGACG 
GTGGGCGCGG CGGTCGCCTG CTTCCTGGCG CTGGAGGGCG CCGACGTGAG CTACACCAAC 
CACCGCGGTC GGAGCCCGCT GGACCTGGCC GCCGAGGGTC GCGTGCTCAA GGCCCTTCAG 
GGCTGCGCCC AGCGCTTCCG GGAGCGGCAG GCGGGCGGGG GCGCGGCCCC GGGCCCCAGG 
CAAACGCTCG GGACCCCCAA CACCGTGACG AACCTGCACG TGGGCGCCGC GCCGGGGCCC 
GAGGCCGCTG AGTGCCTGGT GTGCTCCGAG CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC 
CAGCACCGCA CCGTGTGTGA GGGTGAGTGG GGGGCCCCGG GGTGGGGAGG CCCGGCTAGT 
AGGGCCGCAG CCAACCGCGC TCTCCTCTTC GCAGAGTGCG CGCGCAGGAT GAAGAAGTGC 
ATCAGGTGCC AGGTGGTCGT CAGCAAGAAA CTGCGCCCAG ACGGCTCTGA GGTGGCGAGC 
GCCGCCCCCG CCCCCGGCCC GCCGCGCCAG CTGGTGGAGG AGCTGCAGAG CCGCTACCGG 
CAGATGGAGG AACGCATCAC CTGCCCCATC TGCATCGACA GCCACATCCG CCTCGTGTTC 
CAGTGCGGCC ACGGCGCATG CGCCCCCTGC GGCTCCGCGC TCAGCGCCTG CCCCATCTGC 
CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000357.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855846.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu83166
Clone ID Related Accession (Same CDS sequence)	XM_017000357.1 , XM_017000357.2
Accession Version	XM_017000357.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017000357.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGC 
AAGCCGGCGG AGCTGCAGCG CAGGGTGAGT GCTGACAGCC AGCCCTTCCA GCACGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACTGATGTC CTGCGGGAGA TGCAGGAAGG CCACGGCGGC 
TGGAACCCCA GGATGGCGGA GTTTATCGGA CAGACGGGCA CCGTGCATCG TATCACGGAC 
CGCGGGGACG TGCGCGTGCA GTTCAACCAC GAGACGCGCT GGACCTTCCA CCCCGGGGCG 
CTCACCAAGG ACACCAGGAA GGCAGGACAG CTTCGTGGGC GGGAGGGAGG CGGCTGGGCT 
AAGATGCTCC TGCACCACTC CTTCTGGGTG GGCGACGTGG TCCGGGTCAT CGGCGACCTT 
GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CGGACGACAT GGCCCCTGCC 
CTGGGCCGCG TCGGGAAGGT GGTGAAAGTG TTTGGAGACG GGAACCTGCG TGTAGCAGTC 
GCTGGTCAGC GGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC CGAGGAGGAT 
GCCAACCTGG ACGTGGCCGA GCGCGCCCGG GAGAACAAAA GCTCACTGAG CGTGGCCCTG 
GACAAGCTTC GGGCCCAGAA GAGTGACCCA GAGCACCCGG GAAGGCTGGT GGTGGAGGTG 
GCGCTGGGTA ACGCAGCCCG GGCTCTGGAC CTGCTGCGGA GGCGCCCAGA GCAGGTGGAC 
ACCAAGAACC AAGGCAGGAC CGCTCTGCAA GTGGCTGCCT ACCTGGGCCA GGTGGAGTTG 
ATACGGCTGC TGCTACAAGC CAGGGCGGGC GTGGACCTGC CGGACGACGA GGGCAACACG 
GCACTGCACT ACGCGGCCCT GGGGAACCAG CCCGAGGCCA CCAGGGTGCT CCTGAGTGCT 
GGGTGCCGGG CGGACGCCAT CAACAGCACC CAGAGCACAG CACTGCACGT GGCCGTGCAG 
AGGGGCTTCC TGGAGGTGGT GCGGGCCCTG TGTGAGCGCG GCTGTGACGT CAACCTGCCC 
GACGCCCACT CGGACACGCC CCTGCACTCC GCCATCTCGG CGGGCACTGG AGCCAGCGGC 
ATTGTCGAGG TCCTCACGGA GGTGCCAAAC ATCGATGTTA CCGCCACCAA CAGCCAGGGT 
TTCACCCTGC TGCACCATGC CTCCCTCAAG GGTCACGCGC TAGCTGTGAG AAAGATTCTG 
GCTCGGGCGC GGCAGCTGGT GGACGCCAAG AAGGAGGACG GCTTCACGGC GCTGCATCTG 
GCTGCCCTCA ACAACCACCG CGAGGTGGCC CAGATCCTCA TCCGGGAGGG CCGCTGTGAC 
GTGAACGTGC GCAACCGGAA GCTGCAGTCC CCGCTGCATC TCGCCGTGCA ACAGGCCCAC 
GTGGGGCTGG TGCCGCTACT GGTGGACGCT GGGTGCAGTG TCAACGCCGA GGACGAGGAG 
GGGGACACAG CCCTGCACGT GGCGCTGCAG CGTCATCAGC TGCTGCCCCT GGTGGCTGAT 
GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGACATGGGG CGCCGGCTGC 
TGGGGCTGCC AGGTGCCAGG AGACGCCTCC CTCGGGCCTG CCCCGGCGCC CGCCCTCACC 
GGCGTCTGTC CTGCCGCCCA GCTACAGGCC TCGGGCCTCC CCGGCAGCGC GGAGCTGACG 
GTGGGCGCGG CGGTCGCCTG CTTCCTGGCG CTGGAGGGCG CCGACGTGAG CTACACCAAC 
CACCGCGGTC GGAGCCCGCT GGACCTGGCC GCCGAGGGTC GCGTGCTCAA GGCCCTTCAG 
GGCTGCGCCC AGCGCTTCCG GGAGCGGCAG GCGGGCGGGG GCGCGGCCCC GGGCCCCAGG 
CAAACGCTCG GGACCCCCAA CACCGTGACG AACCTGCACG TGGGCGCCGC GCCGGGGCCC 
GAGGCCGCTG AGTGCCTGGT GTGCTCCGAG CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC 
CAGCACCGCA CCGTGTGTGA GGGTGAGTGG GGGGCCCCGG GGTGGGGAGG CCCGGCTAGT 
AGGGCCGCAG CCAACCGCGC TCTCCTCTTC GCAGAGTGCG CGCGCAGGAT GAAGAAGTGC 
ATCAGGTGCC AGGTGGTCGT CAGCAAGAAA CTGCGCCCAG ACGGCTCTGA GGTGGCGAGC 
GCCGCCCCCG CCCCCGGCCC GCCGCGCCAG CTGGTGGAGG AGCTGCAGAG CCGCTACCGG 
CAGATGGAGG AACGCATCAC CTGCCCCATC TGCATCGACA GCCACATCCG CCTCGTGTTC 
CAGTGCGGCC ACGGCGCATG CGCCCCCTGC GGCTCCGCGC TCAGCGCCTG CCCCATCTGC 
CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855846.1
CDS	1731..5429
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMVRAGADLLPWSSAPSPSRSPLWQNASAVRFPLPLAQQPRAGLGVGSARDGLGLRAQRRRGASWELWSGHPRGTPAPGRPRAARGQRRWGRRWGRRPGRVGCGARQALWPGLAAAEQSGPVGGLERRDRPTDGLAGGPAPPSGAGGTETSGQVGVGRVGSPARLEASPKVSRHQGCSPGASRRPGGRLDGRTGPEQPGPPWTPLPTGPEQPRPTWTQTPRRACRWACGWCAAWTGSGASRTAARAAWARWWSLAATAAPRHPTAQWSCSGTRARAPTTAPATRARTTCCCVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000357.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000357.2

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGTGCGT 
GCGGGCGCGG ATCTCCTCCC CTGGTCCTCC GCACCGTCCC CGAGTCGTTC TCCGCTCTGG 
CAGAACGCGA GCGCCGTCCG GTTCCCGCTC CCGCTGGCCC AGCAGCCCCG GGCTGGACTC 
GGCGTAGGGT CCGCACGGGA TGGGCTGGGG CTGCGCGCGC AGCGCCGCCG CGGGGCCTCC 
TGGGAGTTGT GGTCCGGCCA CCCGCGCGGG ACTCCAGCTC CCGGCAGGCC TCGCGCGGCC 
CGGGGCCAGC GGCGCTGGGG TCGGCGCTGG GGTCGTCGTC CGGGCCGGGT GGGCTGCGGC 
GCGCGGCAGG CGCTCTGGCC TGGCCTGGCC GCCGCTGAGC AGTCGGGGCC GGTGGGTGGC 
CTCGAACGCC GGGACAGACC GACAGACGGA CTGGCCGGCG GTCCCGCGCC CCCGAGCGGA 
GCGGGCGGGA CGGAAACGTC CGGGCAAGTT GGGGTCGGCA GGGTTGGAAG CCCAGCGAGG 
CTAGAGGCCA GTCCCAAAGT TTCCAGGCAT CAGGGCTGCA GCCCAGGAGC CTCAAGGCGG 
CCCGGCGGGC GACTGGACGG CCGGACAGGT CCCGAGCAGC CCGGCCCACC ATGGACCCCT 
CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG GCGGGCGTGC 
AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG GACGGCGGCG 
AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG ACACCCGACC 
GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC GGCTACCAGG 
GCGCGCACGA CCTGCTGCTG CGTCCGGCAC CCCAACATCA TCTGTGACTG CTGCAAGAAG 
CACGGGCTGC GGGGGATGCG CTGGAAGTGC CGTGTGTGCC TGGACTACGA CCTCTGCACG 
CAGTGCTACA TGCACAACAA GCATGAGCTC GCCCACGCCT TCGACCGCTA CGAGACCGCT 
CACTCGCGCC CTGTCACACT GAGTCCCCGC CAGGGCCTCC CGAGGATCCC ACTAAGGGGC 
ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC CCCGACTGGG AGTGGGGCTC ACAGGATGGC 
AAGCCGGCGG AGCTGCAGCG CAGGGTGAGT GCTGACAGCC AGCCCTTCCA GCACGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACTGATGTC CTGCGGGAGA TGCAGGAAGG CCACGGCGGC 
TGGAACCCCA GGATGGCGGA GTTTATCGGA CAGACGGGCA CCGTGCATCG TATCACGGAC 
CGCGGGGACG TGCGCGTGCA GTTCAACCAC GAGACGCGCT GGACCTTCCA CCCCGGGGCG 
CTCACCAAGG ACACCAGGAA GGCAGGACAG CTTCGTGGGC GGGAGGGAGG CGGCTGGGCT 
AAGATGCTCC TGCACCACTC CTTCTGGGTG GGCGACGTGG TCCGGGTCAT CGGCGACCTT 
GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CGGACGACAT GGCCCCTGCC 
CTGGGCCGCG TCGGGAAGGT GGTGAAAGTG TTTGGAGACG GGAACCTGCG TGTAGCAGTC 
GCTGGTCAGC GGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC CGAGGAGGAT 
GCCAACCTGG ACGTGGCCGA GCGCGCCCGG GAGAACAAAA GCTCACTGAG CGTGGCCCTG 
GACAAGCTTC GGGCCCAGAA GAGTGACCCA GAGCACCCGG GAAGGCTGGT GGTGGAGGTG 
GCGCTGGGTA ACGCAGCCCG GGCTCTGGAC CTGCTGCGGA GGCGCCCAGA GCAGGTGGAC 
ACCAAGAACC AAGGCAGGAC CGCTCTGCAA GTGGCTGCCT ACCTGGGCCA GGTGGAGTTG 
ATACGGCTGC TGCTACAAGC CAGGGCGGGC GTGGACCTGC CGGACGACGA GGGCAACACG 
GCACTGCACT ACGCGGCCCT GGGGAACCAG CCCGAGGCCA CCAGGGTGCT CCTGAGTGCT 
GGGTGCCGGG CGGACGCCAT CAACAGCACC CAGAGCACAG CACTGCACGT GGCCGTGCAG 
AGGGGCTTCC TGGAGGTGGT GCGGGCCCTG TGTGAGCGCG GCTGTGACGT CAACCTGCCC 
GACGCCCACT CGGACACGCC CCTGCACTCC GCCATCTCGG CGGGCACTGG AGCCAGCGGC 
ATTGTCGAGG TCCTCACGGA GGTGCCAAAC ATCGATGTTA CCGCCACCAA CAGCCAGGGT 
TTCACCCTGC TGCACCATGC CTCCCTCAAG GGTCACGCGC TAGCTGTGAG AAAGATTCTG 
GCTCGGGCGC GGCAGCTGGT GGACGCCAAG AAGGAGGACG GCTTCACGGC GCTGCATCTG 
GCTGCCCTCA ACAACCACCG CGAGGTGGCC CAGATCCTCA TCCGGGAGGG CCGCTGTGAC 
GTGAACGTGC GCAACCGGAA GCTGCAGTCC CCGCTGCATC TCGCCGTGCA ACAGGCCCAC 
GTGGGGCTGG TGCCGCTACT GGTGGACGCT GGGTGCAGTG TCAACGCCGA GGACGAGGAG 
GGGGACACAG CCCTGCACGT GGCGCTGCAG CGTCATCAGC TGCTGCCCCT GGTGGCTGAT 
GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGACATGGGG CGCCGGCTGC 
TGGGGCTGCC AGGTGCCAGG AGACGCCTCC CTCGGGCCTG CCCCGGCGCC CGCCCTCACC 
GGCGTCTGTC CTGCCGCCCA GCTACAGGCC TCGGGCCTCC CCGGCAGCGC GGAGCTGACG 
GTGGGCGCGG CGGTCGCCTG CTTCCTGGCG CTGGAGGGCG CCGACGTGAG CTACACCAAC 
CACCGCGGTC GGAGCCCGCT GGACCTGGCC GCCGAGGGTC GCGTGCTCAA GGCCCTTCAG 
GGCTGCGCCC AGCGCTTCCG GGAGCGGCAG GCGGGCGGGG GCGCGGCCCC GGGCCCCAGG 
CAAACGCTCG GGACCCCCAA CACCGTGACG AACCTGCACG TGGGCGCCGC GCCGGGGCCC 
GAGGCCGCTG AGTGCCTGGT GTGCTCCGAG CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC 
CAGCACCGCA CCGTGTGTGA GGGTGAGTGG GGGGCCCCGG GGTGGGGAGG CCCGGCTAGT 
AGGGCCGCAG CCAACCGCGC TCTCCTCTTC GCAGAGTGCG CGCGCAGGAT GAAGAAGTGC 
ATCAGGTGCC AGGTGGTCGT CAGCAAGAAA CTGCGCCCAG ACGGCTCTGA GGTGGCGAGC 
GCCGCCCCCG CCCCCGGCCC GCCGCGCCAG CTGGTGGAGG AGCTGCAGAG CCGCTACCGG 
CAGATGGAGG AACGCATCAC CTGCCCCATC TGCATCGACA GCCACATCCG CCTCGTGTTC 
CAGTGCGGCC ACGGCGCATG CGCCCCCTGC GGCTCCGCGC TCAGCGCCTG CCCCATCTGC 
CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000358.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855847.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu83167
Clone ID Related Accession (Same CDS sequence)	XM_017000358.1
Accession Version	XM_017000358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGAGGGGAAG GGAAACCGGG CCGTGTGGTG 
GACATCCGTG GCTGGGATGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC GTGGGCTGAT 
GGTACCACCA ATGTGTACCG TGTGGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCGG GCGGCTTCTA CTACAAGGAC CACCTCCCAA GGCTCGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGT TTATCGGACA GACGGGCACC GTGCATCGTA TCACGGACCG CGGGGACGTG 
CGCGTGCAGT TCAACCACGA GACGCGCTGG ACCTTCCACC CCGGGGCGCT CACCAAGGAC 
ACCAGGAAGG CAGGACAGCT TCGTGGGCGG GAGGGAGGCG GCTGGGCTAA GATGCTCCTG 
CACCACTCCT TCTGGGTGGG CGACGTGGTC CGGGTCATCG GCGACCTTGA CACAGTGAAG 
CGGCTGCAGG CTGGGCATGG CGAGTGGACG GACGACATGG CCCCTGCCCT GGGCCGCGTC 
GGGAAGGTGG TGAAAGTGTT TGGAGACGGG AACCTGCGTG TAGCAGTCGC TGGTCAGCGG 
TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCCG AGGAGGATGC CAACCTGGAC 
GTGGCCGAGC GCGCCCGGGA GAACAAAAGC TCACTGAGCG TGGCCCTGGA CAAGCTTCGG 
GCCCAGAAGA GTGACCCAGA GCACCCGGGA AGGCTGGTGG TGGAGGTGGC GCTGGGTAAC 
GCAGCCCGGG CTCTGGACCT GCTGCGGAGG CGCCCAGAGC AGGTGGACAC CAAGAACCAA 
GGCAGGACCG CTCTGCAAGT GGCTGCCTAC CTGGGCCAGG TGGAGTTGAT ACGGCTGCTG 
CTACAAGCCA GGGCGGGCGT GGACCTGCCG GACGACGAGG GCAACACGGC ACTGCACTAC 
GCGGCCCTGG GGAACCAGCC CGAGGCCACC AGGGTGCTCC TGAGTGCTGG GTGCCGGGCG 
GACGCCATCA ACAGCACCCA GAGCACAGCA CTGCACGTGG CCGTGCAGAG GGGCTTCCTG 
GAGGTGGTGC GGGCCCTGTG TGAGCGCGGC TGTGACGTCA ACCTGCCCGA CGCCCACTCG 
GACACGCCCC TGCACTCCGC CATCTCGGCG GGCACTGGAG CCAGCGGCAT TGTCGAGGTC 
CTCACGGAGG TGCCAAACAT CGATGTTACC GCCACCAACA GCCAGGGTTT CACCCTGCTG 
CACCATGCCT CCCTCAAGGG TCACGCGCTA GCTGTGAGAA AGATTCTGGC TCGGGCGCGG 
CAGCTGGTGG ACGCCAAGAA GGAGGACGGC TTCACGGCGC TGCATCTGGC TGCCCTCAAC 
AACCACCGCG AGGTGGCCCA GATCCTCATC CGGGAGGGCC GCTGTGACGT GAACGTGCGC 
AACCGGAAGC TGCAGTCCCC GCTGCATCTC GCCGTGCAAC AGGCCCACGT GGGGCTGGTG 
CCGCTACTGG TGGACGCTGG GTGCAGTGTC AACGCCGAGG ACGAGGAGGG GGACACAGCC 
CTGCACGTGG CGCTGCAGCG TCATCAGCTG CTGCCCCTGG TGGCTGATGG GGCCGGGGGG 
GACCCAGGGC CCTTGCAGCT GCTGTCCAGG ACATGGGGCG CCGGCTGCTG GGGCTGCCAG 
GTGCCAGGAG ACGCCTCCCT CGGGCCTGCC CCGGCGCCCG CCCTCACCGG CGTCTGTCCT 
GCCGCCCAGC TACAGGCCTC GGGCCTCCCC GGCAGCGCGG AGCTGACGGT GGGCGCGGCG 
GTCGCCTGCT TCCTGGCGCT GGAGGGCGCC GACGTGAGCT ACACCAACCA CCGCGGTCGG 
AGCCCGCTGG ACCTGGCCGC CGAGGGTCGC GTGCTCAAGG CCCTTCAGGG CTGCGCCCAG 
CGCTTCCGGG AGCGGCAGGC GGGCGGGGGC GCGGCCCCGG GCCCCAGGCA AACGCTCGGG 
ACCCCCAACA CCGTGACGAA CCTGCACGTG GGCGCCGCGC CGGGGCCCGA GGCCGCTGAG 
TGCCTGGTGT GCTCCGAGCT GGCGCTGCTG GTGCTGTTCT CGCCGTGCCA GCACCGCACC 
GTGTGTGAGG GTGAGTGGGG GGCCCCGGGG TGGGGAGGCC CGGCTAGTAG GGCCGCAGCC 
AACCGCGCTC TCCTCTTCGC AGAGTGCGCG CGCAGGATGA AGAAGTGCAT CAGGTGCCAG 
GTGGTCGTCA GCAAGAAACT GCGCCCAGAC GGCTCTGAGG TGGCGAGCGC CGCCCCCGCC 
CCCGGCCCGC CGCGCCAGCT GGTGGAGGAG CTGCAGAGCC GCTACCGGCA GATGGAGGAA 
CGCATCACCT GCCCCATCTG CATCGACAGC CACATCCGCC TCGTGTTCCA GTGCGGCCAC 
GGCGCATGCG CCCCCTGCGG CTCCGCGCTC AGCGCCTGCC CCATCTGCCG CCAGCCCATC 
CGCGACCGCA TCCAGATCTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855847.1
CDS	18..3464
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAARPTMDPSAHRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000358.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000358.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGAGGGGAAG GGAAACCGGG CCGTGTGGTG 
GACATCCGTG GCTGGGATGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC GTGGGCTGAT 
GGTACCACCA ATGTGTACCG TGTGGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCGG GCGGCTTCTA CTACAAGGAC CACCTCCCAA GGCTCGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGT TTATCGGACA GACGGGCACC GTGCATCGTA TCACGGACCG CGGGGACGTG 
CGCGTGCAGT TCAACCACGA GACGCGCTGG ACCTTCCACC CCGGGGCGCT CACCAAGGAC 
ACCAGGAAGG CAGGACAGCT TCGTGGGCGG GAGGGAGGCG GCTGGGCTAA GATGCTCCTG 
CACCACTCCT TCTGGGTGGG CGACGTGGTC CGGGTCATCG GCGACCTTGA CACAGTGAAG 
CGGCTGCAGG CTGGGCATGG CGAGTGGACG GACGACATGG CCCCTGCCCT GGGCCGCGTC 
GGGAAGGTGG TGAAAGTGTT TGGAGACGGG AACCTGCGTG TAGCAGTCGC TGGTCAGCGG 
TGGACCTTCA GCCCCTCCTG CCTGGTGGCC TACCGGCCCG AGGAGGATGC CAACCTGGAC 
GTGGCCGAGC GCGCCCGGGA GAACAAAAGC TCACTGAGCG TGGCCCTGGA CAAGCTTCGG 
GCCCAGAAGA GTGACCCAGA GCACCCGGGA AGGCTGGTGG TGGAGGTGGC GCTGGGTAAC 
GCAGCCCGGG CTCTGGACCT GCTGCGGAGG CGCCCAGAGC AGGTGGACAC CAAGAACCAA 
GGCAGGACCG CTCTGCAAGT GGCTGCCTAC CTGGGCCAGG TGGAGTTGAT ACGGCTGCTG 
CTACAAGCCA GGGCGGGCGT GGACCTGCCG GACGACGAGG GCAACACGGC ACTGCACTAC 
GCGGCCCTGG GGAACCAGCC CGAGGCCACC AGGGTGCTCC TGAGTGCTGG GTGCCGGGCG 
GACGCCATCA ACAGCACCCA GAGCACAGCA CTGCACGTGG CCGTGCAGAG GGGCTTCCTG 
GAGGTGGTGC GGGCCCTGTG TGAGCGCGGC TGTGACGTCA ACCTGCCCGA CGCCCACTCG 
GACACGCCCC TGCACTCCGC CATCTCGGCG GGCACTGGAG CCAGCGGCAT TGTCGAGGTC 
CTCACGGAGG TGCCAAACAT CGATGTTACC GCCACCAACA GCCAGGGTTT CACCCTGCTG 
CACCATGCCT CCCTCAAGGG TCACGCGCTA GCTGTGAGAA AGATTCTGGC TCGGGCGCGG 
CAGCTGGTGG ACGCCAAGAA GGAGGACGGC TTCACGGCGC TGCATCTGGC TGCCCTCAAC 
AACCACCGCG AGGTGGCCCA GATCCTCATC CGGGAGGGCC GCTGTGACGT GAACGTGCGC 
AACCGGAAGC TGCAGTCCCC GCTGCATCTC GCCGTGCAAC AGGCCCACGT GGGGCTGGTG 
CCGCTACTGG TGGACGCTGG GTGCAGTGTC AACGCCGAGG ACGAGGAGGG GGACACAGCC 
CTGCACGTGG CGCTGCAGCG TCATCAGCTG CTGCCCCTGG TGGCTGATGG GGCCGGGGGG 
GACCCAGGGC CCTTGCAGCT GCTGTCCAGG ACATGGGGCG CCGGCTGCTG GGGCTGCCAG 
GTGCCAGGAG ACGCCTCCCT CGGGCCTGCC CCGGCGCCCG CCCTCACCGG CGTCTGTCCT 
GCCGCCCAGC TACAGGCCTC GGGCCTCCCC GGCAGCGCGG AGCTGACGGT GGGCGCGGCG 
GTCGCCTGCT TCCTGGCGCT GGAGGGCGCC GACGTGAGCT ACACCAACCA CCGCGGTCGG 
AGCCCGCTGG ACCTGGCCGC CGAGGGTCGC GTGCTCAAGG CCCTTCAGGG CTGCGCCCAG 
CGCTTCCGGG AGCGGCAGGC GGGCGGGGGC GCGGCCCCGG GCCCCAGGCA AACGCTCGGG 
ACCCCCAACA CCGTGACGAA CCTGCACGTG GGCGCCGCGC CGGGGCCCGA GGCCGCTGAG 
TGCCTGGTGT GCTCCGAGCT GGCGCTGCTG GTGCTGTTCT CGCCGTGCCA GCACCGCACC 
GTGTGTGAGG GTGAGTGGGG GGCCCCGGGG TGGGGAGGCC CGGCTAGTAG GGCCGCAGCC 
AACCGCGCTC TCCTCTTCGC AGAGTGCGCG CGCAGGATGA AGAAGTGCAT CAGGTGCCAG 
GTGGTCGTCA GCAAGAAACT GCGCCCAGAC GGCTCTGAGG TGGCGAGCGC CGCCCCCGCC 
CCCGGCCCGC CGCGCCAGCT GGTGGAGGAG CTGCAGAGCC GCTACCGGCA GATGGAGGAA 
CGCATCACCT GCCCCATCTG CATCGACAGC CACATCCGCC TCGTGTTCCA GTGCGGCCAC 
GGCGCATGCG CCCCCTGCGG CTCCGCGCTC AGCGCCTGCC CCATCTGCCG CCAGCCCATC 
CGCGACCGCA TCCAGATCTT CGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000359.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855848.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu83168
Clone ID Related Accession (Same CDS sequence)	XM_017000359.1
Accession Version	XM_017000359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGAGGGGAAG GGAAACCGGG CCGTGTGGTG 
GACATCCGTG GCTGGGATGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC GTGGGCTGAT 
GGTACCACCA ATGTGTACCG TGTGGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCGG GCGGCTTCTA CTACAAGGAC CACCTCCCAA GGCTCGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGA CGGGCACCGT GCATCGTATC ACGGACCGCG GGGACGTGCG CGTGCAGTTC 
AACCACGAGA CGCGCTGGAC CTTCCACCCC GGGGCGCTCA CCAAGGACAC CAGGAAGGCA 
GGACAGCTTC GTGGGCGGGA GGGAGGCGGC TGGGCTAAGA TGCTCCTGCA CCACTCCTTC 
TGGGTGGGCG ACGTGGTCCG GGTCATCGGC GACCTTGACA CAGTGAAGCG GCTGCAGGCT 
GGGCATGGCG AGTGGACGGA CGACATGGCC CCTGCCCTGG GCCGCGTCGG GAAGGTGGTG 
AAAGTGTTTG GAGACGGGAA CCTGCGTGTA GCAGTCGCTG GTCAGCGGTG GACCTTCAGC 
CCCTCCTGCC TGGTGGCCTA CCGGCCCGAG GAGGATGCCA ACCTGGACGT GGCCGAGCGC 
GCCCGGGAGA ACAAAAGCTC ACTGAGCGTG GCCCTGGACA AGCTTCGGGC CCAGAAGAGT 
GACCCAGAGC ACCCGGGAAG GCTGGTGGTG GAGGTGGCGC TGGGTAACGC AGCCCGGGCT 
CTGGACCTGC TGCGGAGGCG CCCAGAGCAG GTGGACACCA AGAACCAAGG CAGGACCGCT 
CTGCAAGTGG CTGCCTACCT GGGCCAGGTG GAGTTGATAC GGCTGCTGCT ACAAGCCAGG 
GCGGGCGTGG ACCTGCCGGA CGACGAGGGC AACACGGCAC TGCACTACGC GGCCCTGGGG 
AACCAGCCCG AGGCCACCAG GGTGCTCCTG AGTGCTGGGT GCCGGGCGGA CGCCATCAAC 
AGCACCCAGA GCACAGCACT GCACGTGGCC GTGCAGAGGG GCTTCCTGGA GGTGGTGCGG 
GCCCTGTGTG AGCGCGGCTG TGACGTCAAC CTGCCCGACG CCCACTCGGA CACGCCCCTG 
CACTCCGCCA TCTCGGCGGG CACTGGAGCC AGCGGCATTG TCGAGGTCCT CACGGAGGTG 
CCAAACATCG ATGTTACCGC CACCAACAGC CAGGGTTTCA CCCTGCTGCA CCATGCCTCC 
CTCAAGGGTC ACGCGCTAGC TGTGAGAAAG ATTCTGGCTC GGGCGCGGCA GCTGGTGGAC 
GCCAAGAAGG AGGACGGCTT CACGGCGCTG CATCTGGCTG CCCTCAACAA CCACCGCGAG 
GTGGCCCAGA TCCTCATCCG GGAGGGCCGC TGTGACGTGA ACGTGCGCAA CCGGAAGCTG 
CAGTCCCCGC TGCATCTCGC CGTGCAACAG GCCCACGTGG GGCTGGTGCC GCTACTGGTG 
GACGCTGGGT GCAGTGTCAA CGCCGAGGAC GAGGAGGGGG ACACAGCCCT GCACGTGGCG 
CTGCAGCGTC ATCAGCTGCT GCCCCTGGTG GCTGATGGGG CCGGGGGGGA CCCAGGGCCC 
TTGCAGCTGC TGTCCAGGAC ATGGGGCGCC GGCTGCTGGG GCTGCCAGGT GCCAGGAGAC 
GCCTCCCTCG GGCCTGCCCC GGCGCCCGCC CTCACCGGCG TCTGTCCTGC CGCCCAGCTA 
CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG CTGACGGTGG GCGCGGCGGT CGCCTGCTTC 
CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC ACCAACCACC GCGGTCGGAG CCCGCTGGAC 
CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG 
CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC CCCAGGCAAA CGCTCGGGAC CCCCAACACC 
GTGACGAACC TGCACGTGGG CGCCGCGCCG GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC 
TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG CCGTGCCAGC ACCGCACCGT GTGTGAGGGT 
GAGTGGGGGG CCCCGGGGTG GGGAGGCCCG GCTAGTAGGG CCGCAGCCAA CCGCGCTCTC 
CTCTTCGCAG AGTGCGCGCG CAGGATGAAG AAGTGCATCA GGTGCCAGGT GGTCGTCAGC 
AAGAAACTGC GCCCAGACGG CTCTGAGGTG GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG 
CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC TACCGGCAGA TGGAGGAACG CATCACCTGC 
CCCATCTGCA TCGACAGCCA CATCCGCCTC GTGTTCCAGT GCGGCCACGG CGCATGCGCC 
CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC ATCTGCCGCC AGCCCATCCG CGACCGCATC 
CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855848.1
CDS	18..3452
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAARPTMDPSAHRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAETGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000359.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000359.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGAGGGGAAG GGAAACCGGG CCGTGTGGTG 
GACATCCGTG GCTGGGATGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC GTGGGCTGAT 
GGTACCACCA ATGTGTACCG TGTGGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCGG GCGGCTTCTA CTACAAGGAC CACCTCCCAA GGCTCGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGA CGGGCACCGT GCATCGTATC ACGGACCGCG GGGACGTGCG CGTGCAGTTC 
AACCACGAGA CGCGCTGGAC CTTCCACCCC GGGGCGCTCA CCAAGGACAC CAGGAAGGCA 
GGACAGCTTC GTGGGCGGGA GGGAGGCGGC TGGGCTAAGA TGCTCCTGCA CCACTCCTTC 
TGGGTGGGCG ACGTGGTCCG GGTCATCGGC GACCTTGACA CAGTGAAGCG GCTGCAGGCT 
GGGCATGGCG AGTGGACGGA CGACATGGCC CCTGCCCTGG GCCGCGTCGG GAAGGTGGTG 
AAAGTGTTTG GAGACGGGAA CCTGCGTGTA GCAGTCGCTG GTCAGCGGTG GACCTTCAGC 
CCCTCCTGCC TGGTGGCCTA CCGGCCCGAG GAGGATGCCA ACCTGGACGT GGCCGAGCGC 
GCCCGGGAGA ACAAAAGCTC ACTGAGCGTG GCCCTGGACA AGCTTCGGGC CCAGAAGAGT 
GACCCAGAGC ACCCGGGAAG GCTGGTGGTG GAGGTGGCGC TGGGTAACGC AGCCCGGGCT 
CTGGACCTGC TGCGGAGGCG CCCAGAGCAG GTGGACACCA AGAACCAAGG CAGGACCGCT 
CTGCAAGTGG CTGCCTACCT GGGCCAGGTG GAGTTGATAC GGCTGCTGCT ACAAGCCAGG 
GCGGGCGTGG ACCTGCCGGA CGACGAGGGC AACACGGCAC TGCACTACGC GGCCCTGGGG 
AACCAGCCCG AGGCCACCAG GGTGCTCCTG AGTGCTGGGT GCCGGGCGGA CGCCATCAAC 
AGCACCCAGA GCACAGCACT GCACGTGGCC GTGCAGAGGG GCTTCCTGGA GGTGGTGCGG 
GCCCTGTGTG AGCGCGGCTG TGACGTCAAC CTGCCCGACG CCCACTCGGA CACGCCCCTG 
CACTCCGCCA TCTCGGCGGG CACTGGAGCC AGCGGCATTG TCGAGGTCCT CACGGAGGTG 
CCAAACATCG ATGTTACCGC CACCAACAGC CAGGGTTTCA CCCTGCTGCA CCATGCCTCC 
CTCAAGGGTC ACGCGCTAGC TGTGAGAAAG ATTCTGGCTC GGGCGCGGCA GCTGGTGGAC 
GCCAAGAAGG AGGACGGCTT CACGGCGCTG CATCTGGCTG CCCTCAACAA CCACCGCGAG 
GTGGCCCAGA TCCTCATCCG GGAGGGCCGC TGTGACGTGA ACGTGCGCAA CCGGAAGCTG 
CAGTCCCCGC TGCATCTCGC CGTGCAACAG GCCCACGTGG GGCTGGTGCC GCTACTGGTG 
GACGCTGGGT GCAGTGTCAA CGCCGAGGAC GAGGAGGGGG ACACAGCCCT GCACGTGGCG 
CTGCAGCGTC ATCAGCTGCT GCCCCTGGTG GCTGATGGGG CCGGGGGGGA CCCAGGGCCC 
TTGCAGCTGC TGTCCAGGAC ATGGGGCGCC GGCTGCTGGG GCTGCCAGGT GCCAGGAGAC 
GCCTCCCTCG GGCCTGCCCC GGCGCCCGCC CTCACCGGCG TCTGTCCTGC CGCCCAGCTA 
CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG CTGACGGTGG GCGCGGCGGT CGCCTGCTTC 
CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC ACCAACCACC GCGGTCGGAG CCCGCTGGAC 
CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG 
CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC CCCAGGCAAA CGCTCGGGAC CCCCAACACC 
GTGACGAACC TGCACGTGGG CGCCGCGCCG GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC 
TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG CCGTGCCAGC ACCGCACCGT GTGTGAGGGT 
GAGTGGGGGG CCCCGGGGTG GGGAGGCCCG GCTAGTAGGG CCGCAGCCAA CCGCGCTCTC 
CTCTTCGCAG AGTGCGCGCG CAGGATGAAG AAGTGCATCA GGTGCCAGGT GGTCGTCAGC 
AAGAAACTGC GCCCAGACGG CTCTGAGGTG GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG 
CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC TACCGGCAGA TGGAGGAACG CATCACCTGC 
CCCATCTGCA TCGACAGCCA CATCCGCCTC GTGTTCCAGT GCGGCCACGG CGCATGCGCC 
CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC ATCTGCCGCC AGCCCATCCG CGACCGCATC 
CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000360.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855849.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X17, mRNA.

Price & Availability↑

CloneID	OHu83169
Clone ID Related Accession (Same CDS sequence)	XM_017000360.1
Accession Version	XM_017000360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCCGCCC 
AACATGGACC CAGACCCCCA GGCGGGCGTG CAGGTGGGCA TGCGGGTGGT GCGCGGCGTG 
GACTGGAAGT GGGGCCAGCA GGACGGCGGC GAGGGCGGCG TGGGCACGGT GGTGGAGCTT 
GGCCGCCACG GCAGCCCCTC GACACCCGAC CGCACAGTGG TCGTGCAGTG GGACCAGGGC 
ACGCGCACCA ACTACCGCGC CGGCTACCAG GGCGCGCACG ACCTGCTGCT GTACGACAAC 
GCCCAGATCG GCGTCCGGCA CCCCAACATC ATCTGTGACT GCTGCAAGAA GCACGGGCTG 
CGGGGGATGC GCTGGAAGTG CCGTGTGTGC CTGGACTACG ACCTCTGCAC GCAGTGCTAC 
ATGCACAACA AGCATGAGCT CGCCCACGCC TTCGACCGCT ACGAGACCGC TCACTCGCGC 
CCTGTCACAC TGAGTCCCCG CCAGGGCCTC CCGAGGATCC CACTAAGGGG CATCTTCCAG 
GGAGCGAAGG TGGTGCGAGG CCCCGACTGG GAGTGGGGCT CACAGGATGG AGGGGAAGGG 
AAACCGGGCC GTGTGGTGGA CATCCGTGGC TGGGATGTGG AGACAGGCCG GAGTGTGGCC 
AGCGTGACGT GGGCTGATGG TACCACCAAT GTGTACCGTG TGGGCCACAA GGGCAAGGTG 
GACCTCAAGT GTGTGGGCGA GGCAGCGGGC GGCTTCTACT ACAAGGACCA CCTCCCAAGG 
CTCGGCAAGC CGGCGGAGCT GCAGCGCAGG GTGAGTGCTG ACAGCCAGCC CTTCCAGCAC 
GGGGACAAGG TCAAGTGTCT GCTGGACACT GATGTCCTGC GGGAGATGCA GGAAGGCCAC 
GGCGGCTGGA ACCCCAGGAT GGCGGAGTTT ATCGGACAGA CGGGCACCGT GCATCGTATC 
ACGGACCGCG GGGACGTGCG CGTGCAGTTC AACCACGAGA CGCGCTGGAC CTTCCACCCC 
GGGGCGCTCA CCAAGGACAC CAGGAAGGCA GGACAGCTTC GTGGGCGGGA GGGAGGCGGC 
TGGGCTAAGA TGCTCCTGCA CCACTCCTTC TGGGTGGGCG ACGTGGTCCG GGTCATCGGC 
GACCTTGACA CAGTGAAGCG GCTGCAGGCT GGGCATGGCG AGTGGACGGA CGACATGGCC 
CCTGCCCTGG GCCGCGTCGG GAAGGTGGTG AAAGTGTTTG GAGACGGGAA CCTGCGTGTA 
GCAGTCGCTG GTCAGCGGTG GACCTTCAGC CCCTCCTGCC TGGTGGCCTA CCGGCCCGAG 
GAGGATGCCA ACCTGGACGT GGCCGAGCGC GCCCGGGAGA ACAAAAGCTC ACTGAGCGTG 
GCCCTGGACA AGCTTCGGGC CCAGAAGAGT GACCCAGAGC ACCCGGGAAG GCTGGTGGTG 
GAGGTGGCGC TGGGTAACGC AGCCCGGGCT CTGGACCTGC TGCGGAGGCG CCCAGAGCAG 
GTGGACACCA AGAACCAAGG CAGGACCGCT CTGCAAGTGG CTGCCTACCT GGGCCAGGTG 
GAGTTGATAC GGCTGCTGCT ACAAGCCAGG GCGGGCGTGG ACCTGCCGGA CGACGAGGGC 
AACACGGCAC TGCACTACGC GGCCCTGGGG AACCAGCCCG AGGCCACCAG GGTGCTCCTG 
AGTGCTGGGT GCCGGGCGGA CGCCATCAAC AGCACCCAGA GCACAGCACT GCACGTGGCC 
GTGCAGAGGG GCTTCCTGGA GGTGGTGCGG GCCCTGTGTG AGCGCGGCTG TGACGTCAAC 
CTGCCCGACG CCCACTCGGA CACGCCCCTG CACTCCGCCA TCTCGGCGGG CACTGGAGCC 
AGCGGCATTG TCGAGGTCCT CACGGAGGTG CCAAACATCG ATGTTACCGC CACCAACAGC 
CAGGGTTTCA CCCTGCTGCA CCATGCCTCC CTCAAGGGTC ACGCGCTAGC TGTGAGAAAG 
ATTCTGGCTC GGGCGCGGCA GCTGGTGGAC GCCAAGAAGG AGGACGGCTT CACGGCGCTG 
CATCTGGCTG CCCTCAACAA CCACCGCGAG GTGGCCCAGA TCCTCATCCG GGAGGGCCGC 
TGTGACGTGA ACGTGCGCAA CCGGAAGCTG CAGTCCCCGC TGCATCTCGC CGTGCAACAG 
GCCCACGTGG GGCTGGTGCC GCTACTGGTG GACGCTGGGT GCAGTGTCAA CGCCGAGGAC 
GAGGAGGGGG ACACAGCCCT GCACGTGGCG CTGCAGCGTC ATCAGCTGCT GCCCCTGGTG 
GCTGATGGGG CCGGGGGGGA CCCAGGGCCC TTGCAGCTGC TGTCCAGGAC ATGGGGCGCC 
GGCTGCTGGG GCTGCCAGGT GCCAGGAGAC GCCTCCCTCG GGCCTGCCCC GGCGCCCGCC 
CTCACCGGCG TCTGTCCTGC CGCCCAGCTA CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG 
CTGACGGTGG GCGCGGCGGT CGCCTGCTTC CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC 
ACCAACCACC GCGGTCGGAG CCCGCTGGAC CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC 
CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC 
CCCAGGCAAA CGCTCGGGAC CCCCAACACC GTGACGAACC TGCACGTGGG CGCCGCGCCG 
GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG 
CCGTGCCAGC ACCGCACCGT GTGTGAGGGT GAGTGGGGGG CCCCGGGGTG GGGAGGCCCG 
GCTAGTAGGG CCGCAGCCAA CCGCGCTCTC CTCTTCGCAG AGTGCGCGCG CAGGATGAAG 
AAGTGCATCA GGTGCCAGGT GGTCGTCAGC AAGAAACTGC GCCCAGACGG CTCTGAGGTG 
GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC 
TACCGGCAGA TGGAGGAACG CATCACCTGC CCCATCTGCA TCGACAGCCA CATCCGCCTC 
GTGTTCCAGT GCGGCCACGG CGCATGCGCC CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC 
ATCTGCCGCC AGCCCATCCG CGACCGCATC CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855849.1
CDS	7..3411
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000360.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000360.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCCGCCC 
AACATGGACC CAGACCCCCA GGCGGGCGTG CAGGTGGGCA TGCGGGTGGT GCGCGGCGTG 
GACTGGAAGT GGGGCCAGCA GGACGGCGGC GAGGGCGGCG TGGGCACGGT GGTGGAGCTT 
GGCCGCCACG GCAGCCCCTC GACACCCGAC CGCACAGTGG TCGTGCAGTG GGACCAGGGC 
ACGCGCACCA ACTACCGCGC CGGCTACCAG GGCGCGCACG ACCTGCTGCT GTACGACAAC 
GCCCAGATCG GCGTCCGGCA CCCCAACATC ATCTGTGACT GCTGCAAGAA GCACGGGCTG 
CGGGGGATGC GCTGGAAGTG CCGTGTGTGC CTGGACTACG ACCTCTGCAC GCAGTGCTAC 
ATGCACAACA AGCATGAGCT CGCCCACGCC TTCGACCGCT ACGAGACCGC TCACTCGCGC 
CCTGTCACAC TGAGTCCCCG CCAGGGCCTC CCGAGGATCC CACTAAGGGG CATCTTCCAG 
GGAGCGAAGG TGGTGCGAGG CCCCGACTGG GAGTGGGGCT CACAGGATGG AGGGGAAGGG 
AAACCGGGCC GTGTGGTGGA CATCCGTGGC TGGGATGTGG AGACAGGCCG GAGTGTGGCC 
AGCGTGACGT GGGCTGATGG TACCACCAAT GTGTACCGTG TGGGCCACAA GGGCAAGGTG 
GACCTCAAGT GTGTGGGCGA GGCAGCGGGC GGCTTCTACT ACAAGGACCA CCTCCCAAGG 
CTCGGCAAGC CGGCGGAGCT GCAGCGCAGG GTGAGTGCTG ACAGCCAGCC CTTCCAGCAC 
GGGGACAAGG TCAAGTGTCT GCTGGACACT GATGTCCTGC GGGAGATGCA GGAAGGCCAC 
GGCGGCTGGA ACCCCAGGAT GGCGGAGTTT ATCGGACAGA CGGGCACCGT GCATCGTATC 
ACGGACCGCG GGGACGTGCG CGTGCAGTTC AACCACGAGA CGCGCTGGAC CTTCCACCCC 
GGGGCGCTCA CCAAGGACAC CAGGAAGGCA GGACAGCTTC GTGGGCGGGA GGGAGGCGGC 
TGGGCTAAGA TGCTCCTGCA CCACTCCTTC TGGGTGGGCG ACGTGGTCCG GGTCATCGGC 
GACCTTGACA CAGTGAAGCG GCTGCAGGCT GGGCATGGCG AGTGGACGGA CGACATGGCC 
CCTGCCCTGG GCCGCGTCGG GAAGGTGGTG AAAGTGTTTG GAGACGGGAA CCTGCGTGTA 
GCAGTCGCTG GTCAGCGGTG GACCTTCAGC CCCTCCTGCC TGGTGGCCTA CCGGCCCGAG 
GAGGATGCCA ACCTGGACGT GGCCGAGCGC GCCCGGGAGA ACAAAAGCTC ACTGAGCGTG 
GCCCTGGACA AGCTTCGGGC CCAGAAGAGT GACCCAGAGC ACCCGGGAAG GCTGGTGGTG 
GAGGTGGCGC TGGGTAACGC AGCCCGGGCT CTGGACCTGC TGCGGAGGCG CCCAGAGCAG 
GTGGACACCA AGAACCAAGG CAGGACCGCT CTGCAAGTGG CTGCCTACCT GGGCCAGGTG 
GAGTTGATAC GGCTGCTGCT ACAAGCCAGG GCGGGCGTGG ACCTGCCGGA CGACGAGGGC 
AACACGGCAC TGCACTACGC GGCCCTGGGG AACCAGCCCG AGGCCACCAG GGTGCTCCTG 
AGTGCTGGGT GCCGGGCGGA CGCCATCAAC AGCACCCAGA GCACAGCACT GCACGTGGCC 
GTGCAGAGGG GCTTCCTGGA GGTGGTGCGG GCCCTGTGTG AGCGCGGCTG TGACGTCAAC 
CTGCCCGACG CCCACTCGGA CACGCCCCTG CACTCCGCCA TCTCGGCGGG CACTGGAGCC 
AGCGGCATTG TCGAGGTCCT CACGGAGGTG CCAAACATCG ATGTTACCGC CACCAACAGC 
CAGGGTTTCA CCCTGCTGCA CCATGCCTCC CTCAAGGGTC ACGCGCTAGC TGTGAGAAAG 
ATTCTGGCTC GGGCGCGGCA GCTGGTGGAC GCCAAGAAGG AGGACGGCTT CACGGCGCTG 
CATCTGGCTG CCCTCAACAA CCACCGCGAG GTGGCCCAGA TCCTCATCCG GGAGGGCCGC 
TGTGACGTGA ACGTGCGCAA CCGGAAGCTG CAGTCCCCGC TGCATCTCGC CGTGCAACAG 
GCCCACGTGG GGCTGGTGCC GCTACTGGTG GACGCTGGGT GCAGTGTCAA CGCCGAGGAC 
GAGGAGGGGG ACACAGCCCT GCACGTGGCG CTGCAGCGTC ATCAGCTGCT GCCCCTGGTG 
GCTGATGGGG CCGGGGGGGA CCCAGGGCCC TTGCAGCTGC TGTCCAGGAC ATGGGGCGCC 
GGCTGCTGGG GCTGCCAGGT GCCAGGAGAC GCCTCCCTCG GGCCTGCCCC GGCGCCCGCC 
CTCACCGGCG TCTGTCCTGC CGCCCAGCTA CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG 
CTGACGGTGG GCGCGGCGGT CGCCTGCTTC CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC 
ACCAACCACC GCGGTCGGAG CCCGCTGGAC CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC 
CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC 
CCCAGGCAAA CGCTCGGGAC CCCCAACACC GTGACGAACC TGCACGTGGG CGCCGCGCCG 
GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG 
CCGTGCCAGC ACCGCACCGT GTGTGAGGGT GAGTGGGGGG CCCCGGGGTG GGGAGGCCCG 
GCTAGTAGGG CCGCAGCCAA CCGCGCTCTC CTCTTCGCAG AGTGCGCGCG CAGGATGAAG 
AAGTGCATCA GGTGCCAGGT GGTCGTCAGC AAGAAACTGC GCCCAGACGG CTCTGAGGTG 
GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC 
TACCGGCAGA TGGAGGAACG CATCACCTGC CCCATCTGCA TCGACAGCCA CATCCGCCTC 
GTGTTCCAGT GCGGCCACGG CGCATGCGCC CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC 
ATCTGCCGCC AGCCCATCCG CGACCGCATC CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000361.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855850.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X18, mRNA.

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CloneID	OHu83170
Clone ID Related Accession (Same CDS sequence)	XM_017000361.1
Accession Version	XM_017000361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3285bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGAGGGGAAG GGAAACCGGG CCGTGTGGTG 
GACATCCGTG GCTGGGATGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC GTGGGCTGAT 
GGTACCACCA ATGTGTACCG TGTGGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCGG GCGGCTTCTA CTACAAGGAC CACCTCCCAA GGCTCGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGT TTATCGGACA GACGGGCACC GTGCATCGTA TCACGGACCG CGGGGACGTG 
CGCGTGCAGT TCAACCACGA GACGCGCTGG ACCTTCCACC CCGGGGCGCT CACCAAGCAC 
CACTCCTTCT GGGTGGGCGA CGTGGTCCGG GTCATCGGCG ACCTTGACAC AGTGAAGCGG 
CTGCAGGCTG GGCATGGCGA GTGGACGGAC GACATGGCCC CTGCCCTGGG CCGCGTCGGG 
AAGGTGGTGA AAGTGTTTGG AGACGGGAAC CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG 
ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC CGGCCCGAGG AGGATGCCAA CCTGGACGTG 
GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA CTGAGCGTGG CCCTGGACAA GCTTCGGGCC 
CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG CTGGTGGTGG AGGTGGCGCT GGGTAACGCA 
GCCCGGGCTC TGGACCTGCT GCGGAGGCGC CCAGAGCAGG TGGACACCAA GAACCAAGGC 
AGGACCGCTC TGCAAGTGGC TGCCTACCTG GGCCAGGTGG AGTTGATACG GCTGCTGCTA 
CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC GACGAGGGCA ACACGGCACT GCACTACGCG 
GCCCTGGGGA ACCAGCCCGA GGCCACCAGG GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC 
GCCATCAACA GCACCCAGAG CACAGCACTG CACGTGGCCG TGCAGAGGGG CTTCCTGGAG 
GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT GACGTCAACC TGCCCGACGC CCACTCGGAC 
ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC ACTGGAGCCA GCGGCATTGT CGAGGTCCTC 
ACGGAGGTGC CAAACATCGA TGTTACCGCC ACCAACAGCC AGGGTTTCAC CCTGCTGCAC 
CATGCCTCCC TCAAGGGTCA CGCGCTAGCT GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG 
CTGGTGGACG CCAAGAAGGA GGACGGCTTC ACGGCGCTGC ATCTGGCTGC CCTCAACAAC 
CACCGCGAGG TGGCCCAGAT CCTCATCCGG GAGGGCCGCT GTGACGTGAA CGTGCGCAAC 
CGGAAGCTGC AGTCCCCGCT GCATCTCGCC GTGCAACAGG CCCACGTGGG GCTGGTGCCG 
CTACTGGTGG ACGCTGGGTG CAGTGTCAAC GCCGAGGACG AGGAGGGGGA CACAGCCCTG 
CACGTGGCGC TGCAGCGTCA TCAGCTGCTG CCCCTGGTGG CTGATGGGGC CGGGGGGGAC 
CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG 
CTGACGGTGG GCGCGGCGGT CGCCTGCTTC CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC 
ACCAACCACC GCGGTCGGAG CCCGCTGGAC CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC 
CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC 
CCCAGGCAAA CGCTCGGGAC CCCCAACACC GTGACGAACC TGCACGTGGG CGCCGCGCCG 
GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG 
CCGTGCCAGC ACCGCACCGT GTGTGAGGGT GAGTGGGGGG CCCCGGGGTG GGGAGGCCCG 
GCTAGTAGGG CCGCAGCCAA CCGCGCTCTC CTCTTCGCAG AGTGCGCGCG CAGGATGAAG 
AAGTGCATCA GGTGCCAGGT GGTCGTCAGC AAGAAACTGC GCCCAGACGG CTCTGAGGTG 
GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC 
TACCGGCAGA TGGAGGAACG CATCACCTGC CCCATCTGCA TCGACAGCCA CATCCGCCTC 
GTGTTCCAGT GCGGCCACGG CGCATGCGCC CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC 
ATCTGCCGCC AGCCCATCCG CGACCGCATC CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855850.1
CDS	18..3302
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAARPTMDPSAHRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000361.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000361.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGAGGGGAAG GGAAACCGGG CCGTGTGGTG 
GACATCCGTG GCTGGGATGT GGAGACAGGC CGGAGTGTGG CCAGCGTGAC GTGGGCTGAT 
GGTACCACCA ATGTGTACCG TGTGGGCCAC AAGGGCAAGG TGGACCTCAA GTGTGTGGGC 
GAGGCAGCGG GCGGCTTCTA CTACAAGGAC CACCTCCCAA GGCTCGGCAA GCCGGCGGAG 
CTGCAGCGCA GGGTGAGTGC TGACAGCCAG CCCTTCCAGC ACGGGGACAA GGTCAAGTGT 
CTGCTGGACA CTGATGTCCT GCGGGAGATG CAGGAAGGCC ACGGCGGCTG GAACCCCAGG 
ATGGCGGAGT TTATCGGACA GACGGGCACC GTGCATCGTA TCACGGACCG CGGGGACGTG 
CGCGTGCAGT TCAACCACGA GACGCGCTGG ACCTTCCACC CCGGGGCGCT CACCAAGCAC 
CACTCCTTCT GGGTGGGCGA CGTGGTCCGG GTCATCGGCG ACCTTGACAC AGTGAAGCGG 
CTGCAGGCTG GGCATGGCGA GTGGACGGAC GACATGGCCC CTGCCCTGGG CCGCGTCGGG 
AAGGTGGTGA AAGTGTTTGG AGACGGGAAC CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG 
ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC CGGCCCGAGG AGGATGCCAA CCTGGACGTG 
GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA CTGAGCGTGG CCCTGGACAA GCTTCGGGCC 
CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG CTGGTGGTGG AGGTGGCGCT GGGTAACGCA 
GCCCGGGCTC TGGACCTGCT GCGGAGGCGC CCAGAGCAGG TGGACACCAA GAACCAAGGC 
AGGACCGCTC TGCAAGTGGC TGCCTACCTG GGCCAGGTGG AGTTGATACG GCTGCTGCTA 
CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC GACGAGGGCA ACACGGCACT GCACTACGCG 
GCCCTGGGGA ACCAGCCCGA GGCCACCAGG GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC 
GCCATCAACA GCACCCAGAG CACAGCACTG CACGTGGCCG TGCAGAGGGG CTTCCTGGAG 
GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT GACGTCAACC TGCCCGACGC CCACTCGGAC 
ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC ACTGGAGCCA GCGGCATTGT CGAGGTCCTC 
ACGGAGGTGC CAAACATCGA TGTTACCGCC ACCAACAGCC AGGGTTTCAC CCTGCTGCAC 
CATGCCTCCC TCAAGGGTCA CGCGCTAGCT GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG 
CTGGTGGACG CCAAGAAGGA GGACGGCTTC ACGGCGCTGC ATCTGGCTGC CCTCAACAAC 
CACCGCGAGG TGGCCCAGAT CCTCATCCGG GAGGGCCGCT GTGACGTGAA CGTGCGCAAC 
CGGAAGCTGC AGTCCCCGCT GCATCTCGCC GTGCAACAGG CCCACGTGGG GCTGGTGCCG 
CTACTGGTGG ACGCTGGGTG CAGTGTCAAC GCCGAGGACG AGGAGGGGGA CACAGCCCTG 
CACGTGGCGC TGCAGCGTCA TCAGCTGCTG CCCCTGGTGG CTGATGGGGC CGGGGGGGAC 
CCAGGGCCCT TGCAGCTGCT GTCCAGGCTA CAGGCCTCGG GCCTCCCCGG CAGCGCGGAG 
CTGACGGTGG GCGCGGCGGT CGCCTGCTTC CTGGCGCTGG AGGGCGCCGA CGTGAGCTAC 
ACCAACCACC GCGGTCGGAG CCCGCTGGAC CTGGCCGCCG AGGGTCGCGT GCTCAAGGCC 
CTTCAGGGCT GCGCCCAGCG CTTCCGGGAG CGGCAGGCGG GCGGGGGCGC GGCCCCGGGC 
CCCAGGCAAA CGCTCGGGAC CCCCAACACC GTGACGAACC TGCACGTGGG CGCCGCGCCG 
GGGCCCGAGG CCGCTGAGTG CCTGGTGTGC TCCGAGCTGG CGCTGCTGGT GCTGTTCTCG 
CCGTGCCAGC ACCGCACCGT GTGTGAGGGT GAGTGGGGGG CCCCGGGGTG GGGAGGCCCG 
GCTAGTAGGG CCGCAGCCAA CCGCGCTCTC CTCTTCGCAG AGTGCGCGCG CAGGATGAAG 
AAGTGCATCA GGTGCCAGGT GGTCGTCAGC AAGAAACTGC GCCCAGACGG CTCTGAGGTG 
GCGAGCGCCG CCCCCGCCCC CGGCCCGCCG CGCCAGCTGG TGGAGGAGCT GCAGAGCCGC 
TACCGGCAGA TGGAGGAACG CATCACCTGC CCCATCTGCA TCGACAGCCA CATCCGCCTC 
GTGTTCCAGT GCGGCCACGG CGCATGCGCC CCCTGCGGCT CCGCGCTCAG CGCCTGCCCC 
ATCTGCCGCC AGCCCATCCG CGACCGCATC CAGATCTTCG TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000362.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855851.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OHu83171
Clone ID Related Accession (Same CDS sequence)	XM_017000362.1 , XM_024453317.1
Accession Version	XM_017000362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCCT CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG 
GCGGGCGTGC AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG 
GACGGCGGCG AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG 
ACACCCGACC GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC 
GGCTACCAGG GCGCGCACGA CCTGCTGCTG TACGACAACG CCCAGATCGG CGTCCGGCAC 
CCCAACATCA TCTGTGACTG CTGCAAGAAG CACGGGCTGC GGGGGATGCG CTGGAAGTGC 
CGTGTGTGCC TGGACTACGA CCTCTGCACG CAGTGCTACA TGCACAACAA GCATGAGCTC 
GCCCACGCCT TCGACCGCTA CGAGACCGCT CACTCGCGCC CTGTCACACT GAGTCCCCGC 
CAGGGCCTCC CGAGGATCCC ACTAAGGGGC ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC 
CCCGACTGGG AGTGGGGCTC ACAGGATGGA GGGGAAGGGA AACCGGGCCG TGTGGTGGAC 
ATCCGTGGCT GGGATGTGGA GACAGGCCGG AGTGTGGCCA GCGTGACGTG GGCTGATGGT 
ACCACCAATG TGTACCGTGT GGGCCACAAG GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG 
GCAGCGGGCG GCTTCTACTA CAAGGACCAC CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG 
CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC TTCCAGCACG GGGACAAGGT CAAGTGTCTG 
CTGGACACTG ATGTCCTGCG GGAGATGCAG GAAGGCCACG GCGGCTGGAA CCCCAGGATG 
GCGGAGTTTA TCGGACAGAC GGGCACCGTG CATCGTATCA CGGACCGCGG GGACGTGCGC 
GTGCAGTTCA ACCACGAGAC GCGCTGGACC TTCCACCCCG GGGCGCTCAC CAAGGACACC 
AGGAAGGCAG GACAGCTTCG TGGGCGGGAG GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC 
CACTCCTTCT GGGTGGGCGA CGTGGTCCGG GTCATCGGCG ACCTTGACAC AGTGAAGCGG 
CTGCAGGCTG GGCATGGCGA GTGGACGGAC GACATGGCCC CTGCCCTGGG CCGCGTCGGG 
AAGGTGGTGA AAGTGTTTGG AGACGGGAAC CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG 
ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC CGGCCCGAGG AGGATGCCAA CCTGGACGTG 
GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA CTGAGCGTGG CCCTGGACAA GCTTCGGGCC 
CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG CTGGTGGTGG AGGTGGCGCT GGGTAACGCA 
GCCCGGGCTC TGGACCTGCT GCGGAGGCGC CCAGAGCAGG TGGACACCAA GAACCAAGGC 
AGGACCGCTC TGCAAGTGGC TGCCTACCTG GGCCAGGTGG AGTTGATACG GCTGCTGCTA 
CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC GACGAGGGCA ACACGGCACT GCACTACGCG 
GCCCTGGGGA ACCAGCCCGA GGCCACCAGG GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC 
GCCATCAACA GCACCCAGAG CACAGCACTG CACGTGGCCG TGCAGAGGGG CTTCCTGGAG 
GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT GACGTCAACC TGCCCGACGC CCACTCGGAC 
ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC ACTGGAGCCA GCGGCATTGT CGAGGTCCTC 
ACGGAGGTGC CAAACATCGA TGTTACCGCC ACCAACAGCC AGGGTTTCAC CCTGCTGCAC 
CATGCCTCCC TCAAGGGTCA CGCGCTAGCT GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG 
CTGGTGGACG CCAAGAAGGA GGACGGCTTC ACGGCGCTGC ATCTGGCTGC CCTCAACAAC 
CACCGCGAGG TGGCCCAGAT CCTCATCCGG GAGGGCCGCT GTGACGTGAA CGTGCGCAAC 
CGGAAGCTGC AGTCCCCGCT GCATCTCGCC GTGCAACAGG CCCACGTGGG GCTGGTGCCG 
CTACTGGTGG ACGCTGGGTG CAGTGTCAAC GCCGAGGACG AGGAGGGGGA CACAGCCCTG 
CACGTGGCGC TGCAGCGTCA TCAGCTGCTG CCCCTGGTGG CTGATGGGGC CGGGGGGGAC 
CCAGGGCCCT TGCAGCTGCT GTCCAGGACA TGGGGCGCCG GCTGCTGGGG CTGCCAGGTG 
CCAGGAGACG CCTCCCTCGG GCCTGCCCCG GCGCCCGCCC TCACCGGCGT CTGTCCTGCC 
GCCCAGCTAC AGGCCTCGGG CCTCCCCGGC AGCGCGGAGC TGACGGTGGG CGCGGCGGTC 
GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC GTGAGCTACA CCAACCACCG CGGTCGGAGC 
CCGCTGGACC TGGCCGCCGA GGGTCGCGTG CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC 
TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG GCCCCGGGCC CCAGGCAAAC GCTCGGGACC 
CCCAACACCG TGACGAACCT GCACGTGGGC GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC 
CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG CTGTTCTCGC CGTGCCAGCA CCGCACCGTG 
TGTGAGGGTG AGTGGGGGGC CCCGGGGTGG GGAGGCCCGG CTAGTAGGGC CGCAGCCAAC 
CGCGCTCTCC TCTTCGCAGA GTGCGCGCGC AGGATGAAGA AGTGCATCAG GTGCCAGGTG 
GTCGTCAGCA AGAAACTGCG CCCAGACGGC TCTGAGGTGG CGAGCGCCGC CCCCGCCCCC 
GGCCCGCCGC GCCAGCTGGT GGAGGAGCTG CAGAGCCGCT ACCGGCAGAT GGAGGAACGC 
ATCACCTGCC CCATCTGCAT CGACAGCCAC ATCCGCCTCG TGTTCCAGTG CGGCCACGGC 
GCATGCGCCC CCTGCGGCTC CGCGCTCAGC GCCTGCCCCA TCTGCCGCCA GCCCATCCGC 
GACCGCATCC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855851.1
CDS	361..3504
Translation	MDPSAHRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000362.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000362.1

ORF Insert Sequence:

ATGGACCCCT CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG 
GCGGGCGTGC AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG 
GACGGCGGCG AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG 
ACACCCGACC GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC 
GGCTACCAGG GCGCGCACGA CCTGCTGCTG TACGACAACG CCCAGATCGG CGTCCGGCAC 
CCCAACATCA TCTGTGACTG CTGCAAGAAG CACGGGCTGC GGGGGATGCG CTGGAAGTGC 
CGTGTGTGCC TGGACTACGA CCTCTGCACG CAGTGCTACA TGCACAACAA GCATGAGCTC 
GCCCACGCCT TCGACCGCTA CGAGACCGCT CACTCGCGCC CTGTCACACT GAGTCCCCGC 
CAGGGCCTCC CGAGGATCCC ACTAAGGGGC ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC 
CCCGACTGGG AGTGGGGCTC ACAGGATGGA GGGGAAGGGA AACCGGGCCG TGTGGTGGAC 
ATCCGTGGCT GGGATGTGGA GACAGGCCGG AGTGTGGCCA GCGTGACGTG GGCTGATGGT 
ACCACCAATG TGTACCGTGT GGGCCACAAG GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG 
GCAGCGGGCG GCTTCTACTA CAAGGACCAC CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG 
CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC TTCCAGCACG GGGACAAGGT CAAGTGTCTG 
CTGGACACTG ATGTCCTGCG GGAGATGCAG GAAGGCCACG GCGGCTGGAA CCCCAGGATG 
GCGGAGTTTA TCGGACAGAC GGGCACCGTG CATCGTATCA CGGACCGCGG GGACGTGCGC 
GTGCAGTTCA ACCACGAGAC GCGCTGGACC TTCCACCCCG GGGCGCTCAC CAAGGACACC 
AGGAAGGCAG GACAGCTTCG TGGGCGGGAG GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC 
CACTCCTTCT GGGTGGGCGA CGTGGTCCGG GTCATCGGCG ACCTTGACAC AGTGAAGCGG 
CTGCAGGCTG GGCATGGCGA GTGGACGGAC GACATGGCCC CTGCCCTGGG CCGCGTCGGG 
AAGGTGGTGA AAGTGTTTGG AGACGGGAAC CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG 
ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC CGGCCCGAGG AGGATGCCAA CCTGGACGTG 
GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA CTGAGCGTGG CCCTGGACAA GCTTCGGGCC 
CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG CTGGTGGTGG AGGTGGCGCT GGGTAACGCA 
GCCCGGGCTC TGGACCTGCT GCGGAGGCGC CCAGAGCAGG TGGACACCAA GAACCAAGGC 
AGGACCGCTC TGCAAGTGGC TGCCTACCTG GGCCAGGTGG AGTTGATACG GCTGCTGCTA 
CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC GACGAGGGCA ACACGGCACT GCACTACGCG 
GCCCTGGGGA ACCAGCCCGA GGCCACCAGG GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC 
GCCATCAACA GCACCCAGAG CACAGCACTG CACGTGGCCG TGCAGAGGGG CTTCCTGGAG 
GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT GACGTCAACC TGCCCGACGC CCACTCGGAC 
ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC ACTGGAGCCA GCGGCATTGT CGAGGTCCTC 
ACGGAGGTGC CAAACATCGA TGTTACCGCC ACCAACAGCC AGGGTTTCAC CCTGCTGCAC 
CATGCCTCCC TCAAGGGTCA CGCGCTAGCT GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG 
CTGGTGGACG CCAAGAAGGA GGACGGCTTC ACGGCGCTGC ATCTGGCTGC CCTCAACAAC 
CACCGCGAGG TGGCCCAGAT CCTCATCCGG GAGGGCCGCT GTGACGTGAA CGTGCGCAAC 
CGGAAGCTGC AGTCCCCGCT GCATCTCGCC GTGCAACAGG CCCACGTGGG GCTGGTGCCG 
CTACTGGTGG ACGCTGGGTG CAGTGTCAAC GCCGAGGACG AGGAGGGGGA CACAGCCCTG 
CACGTGGCGC TGCAGCGTCA TCAGCTGCTG CCCCTGGTGG CTGATGGGGC CGGGGGGGAC 
CCAGGGCCCT TGCAGCTGCT GTCCAGGACA TGGGGCGCCG GCTGCTGGGG CTGCCAGGTG 
CCAGGAGACG CCTCCCTCGG GCCTGCCCCG GCGCCCGCCC TCACCGGCGT CTGTCCTGCC 
GCCCAGCTAC AGGCCTCGGG CCTCCCCGGC AGCGCGGAGC TGACGGTGGG CGCGGCGGTC 
GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC GTGAGCTACA CCAACCACCG CGGTCGGAGC 
CCGCTGGACC TGGCCGCCGA GGGTCGCGTG CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC 
TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG GCCCCGGGCC CCAGGCAAAC GCTCGGGACC 
CCCAACACCG TGACGAACCT GCACGTGGGC GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC 
CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG CTGTTCTCGC CGTGCCAGCA CCGCACCGTG 
TGTGAGGGTG AGTGGGGGGC CCCGGGGTGG GGAGGCCCGG CTAGTAGGGC CGCAGCCAAC 
CGCGCTCTCC TCTTCGCAGA GTGCGCGCGC AGGATGAAGA AGTGCATCAG GTGCCAGGTG 
GTCGTCAGCA AGAAACTGCG CCCAGACGGC TCTGAGGTGG CGAGCGCCGC CCCCGCCCCC 
GGCCCGCCGC GCCAGCTGGT GGAGGAGCTG CAGAGCCGCT ACCGGCAGAT GGAGGAACGC 
ATCACCTGCC CCATCTGCAT CGACAGCCAC ATCCGCCTCG TGTTCCAGTG CGGCCACGGC 
GCATGCGCCC CCTGCGGCTC CGCGCTCAGC GCCTGCCCCA TCTGCCGCCA GCCCATCCGC 
GACCGCATCC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_024453317.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024309085.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X20, mRNA.

Price & Availability↑

CloneID	OHu83171
Clone ID Related Accession (Same CDS sequence)	XM_017000362.1 , XM_024453317.1
Accession Version	XM_024453317.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3144bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCCT CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG 
GCGGGCGTGC AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG 
GACGGCGGCG AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG 
ACACCCGACC GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC 
GGCTACCAGG GCGCGCACGA CCTGCTGCTG TACGACAACG CCCAGATCGG CGTCCGGCAC 
CCCAACATCA TCTGTGACTG CTGCAAGAAG CACGGGCTGC GGGGGATGCG CTGGAAGTGC 
CGTGTGTGCC TGGACTACGA CCTCTGCACG CAGTGCTACA TGCACAACAA GCATGAGCTC 
GCCCACGCCT TCGACCGCTA CGAGACCGCT CACTCGCGCC CTGTCACACT GAGTCCCCGC 
CAGGGCCTCC CGAGGATCCC ACTAAGGGGC ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC 
CCCGACTGGG AGTGGGGCTC ACAGGATGGA GGGGAAGGGA AACCGGGCCG TGTGGTGGAC 
ATCCGTGGCT GGGATGTGGA GACAGGCCGG AGTGTGGCCA GCGTGACGTG GGCTGATGGT 
ACCACCAATG TGTACCGTGT GGGCCACAAG GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG 
GCAGCGGGCG GCTTCTACTA CAAGGACCAC CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG 
CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC TTCCAGCACG GGGACAAGGT CAAGTGTCTG 
CTGGACACTG ATGTCCTGCG GGAGATGCAG GAAGGCCACG GCGGCTGGAA CCCCAGGATG 
GCGGAGTTTA TCGGACAGAC GGGCACCGTG CATCGTATCA CGGACCGCGG GGACGTGCGC 
GTGCAGTTCA ACCACGAGAC GCGCTGGACC TTCCACCCCG GGGCGCTCAC CAAGGACACC 
AGGAAGGCAG GACAGCTTCG TGGGCGGGAG GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC 
CACTCCTTCT GGGTGGGCGA CGTGGTCCGG GTCATCGGCG ACCTTGACAC AGTGAAGCGG 
CTGCAGGCTG GGCATGGCGA GTGGACGGAC GACATGGCCC CTGCCCTGGG CCGCGTCGGG 
AAGGTGGTGA AAGTGTTTGG AGACGGGAAC CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG 
ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC CGGCCCGAGG AGGATGCCAA CCTGGACGTG 
GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA CTGAGCGTGG CCCTGGACAA GCTTCGGGCC 
CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG CTGGTGGTGG AGGTGGCGCT GGGTAACGCA 
GCCCGGGCTC TGGACCTGCT GCGGAGGCGC CCAGAGCAGG TGGACACCAA GAACCAAGGC 
AGGACCGCTC TGCAAGTGGC TGCCTACCTG GGCCAGGTGG AGTTGATACG GCTGCTGCTA 
CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC GACGAGGGCA ACACGGCACT GCACTACGCG 
GCCCTGGGGA ACCAGCCCGA GGCCACCAGG GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC 
GCCATCAACA GCACCCAGAG CACAGCACTG CACGTGGCCG TGCAGAGGGG CTTCCTGGAG 
GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT GACGTCAACC TGCCCGACGC CCACTCGGAC 
ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC ACTGGAGCCA GCGGCATTGT CGAGGTCCTC 
ACGGAGGTGC CAAACATCGA TGTTACCGCC ACCAACAGCC AGGGTTTCAC CCTGCTGCAC 
CATGCCTCCC TCAAGGGTCA CGCGCTAGCT GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG 
CTGGTGGACG CCAAGAAGGA GGACGGCTTC ACGGCGCTGC ATCTGGCTGC CCTCAACAAC 
CACCGCGAGG TGGCCCAGAT CCTCATCCGG GAGGGCCGCT GTGACGTGAA CGTGCGCAAC 
CGGAAGCTGC AGTCCCCGCT GCATCTCGCC GTGCAACAGG CCCACGTGGG GCTGGTGCCG 
CTACTGGTGG ACGCTGGGTG CAGTGTCAAC GCCGAGGACG AGGAGGGGGA CACAGCCCTG 
CACGTGGCGC TGCAGCGTCA TCAGCTGCTG CCCCTGGTGG CTGATGGGGC CGGGGGGGAC 
CCAGGGCCCT TGCAGCTGCT GTCCAGGACA TGGGGCGCCG GCTGCTGGGG CTGCCAGGTG 
CCAGGAGACG CCTCCCTCGG GCCTGCCCCG GCGCCCGCCC TCACCGGCGT CTGTCCTGCC 
GCCCAGCTAC AGGCCTCGGG CCTCCCCGGC AGCGCGGAGC TGACGGTGGG CGCGGCGGTC 
GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC GTGAGCTACA CCAACCACCG CGGTCGGAGC 
CCGCTGGACC TGGCCGCCGA GGGTCGCGTG CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC 
TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG GCCCCGGGCC CCAGGCAAAC GCTCGGGACC 
CCCAACACCG TGACGAACCT GCACGTGGGC GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC 
CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG CTGTTCTCGC CGTGCCAGCA CCGCACCGTG 
TGTGAGGGTG AGTGGGGGGC CCCGGGGTGG GGAGGCCCGG CTAGTAGGGC CGCAGCCAAC 
CGCGCTCTCC TCTTCGCAGA GTGCGCGCGC AGGATGAAGA AGTGCATCAG GTGCCAGGTG 
GTCGTCAGCA AGAAACTGCG CCCAGACGGC TCTGAGGTGG CGAGCGCCGC CCCCGCCCCC 
GGCCCGCCGC GCCAGCTGGT GGAGGAGCTG CAGAGCCGCT ACCGGCAGAT GGAGGAACGC 
ATCACCTGCC CCATCTGCAT CGACAGCCAC ATCCGCCTCG TGTTCCAGTG CGGCCACGGC 
GCATGCGCCC CCTGCGGCTC CGCGCTCAGC GCCTGCCCCA TCTGCCGCCA GCCCATCCGC 
GACCGCATCC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024309085.1
CDS	326..3469
Translation	MDPSAHRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_024453317.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024453317.1

ORF Insert Sequence:

ATGGACCCCT CTGCCCACAG GTCCCGAGCA GCCCCGCCCA ACATGGACCC AGACCCCCAG 
GCGGGCGTGC AGGTGGGCAT GCGGGTGGTG CGCGGCGTGG ACTGGAAGTG GGGCCAGCAG 
GACGGCGGCG AGGGCGGCGT GGGCACGGTG GTGGAGCTTG GCCGCCACGG CAGCCCCTCG 
ACACCCGACC GCACAGTGGT CGTGCAGTGG GACCAGGGCA CGCGCACCAA CTACCGCGCC 
GGCTACCAGG GCGCGCACGA CCTGCTGCTG TACGACAACG CCCAGATCGG CGTCCGGCAC 
CCCAACATCA TCTGTGACTG CTGCAAGAAG CACGGGCTGC GGGGGATGCG CTGGAAGTGC 
CGTGTGTGCC TGGACTACGA CCTCTGCACG CAGTGCTACA TGCACAACAA GCATGAGCTC 
GCCCACGCCT TCGACCGCTA CGAGACCGCT CACTCGCGCC CTGTCACACT GAGTCCCCGC 
CAGGGCCTCC CGAGGATCCC ACTAAGGGGC ATCTTCCAGG GAGCGAAGGT GGTGCGAGGC 
CCCGACTGGG AGTGGGGCTC ACAGGATGGA GGGGAAGGGA AACCGGGCCG TGTGGTGGAC 
ATCCGTGGCT GGGATGTGGA GACAGGCCGG AGTGTGGCCA GCGTGACGTG GGCTGATGGT 
ACCACCAATG TGTACCGTGT GGGCCACAAG GGCAAGGTGG ACCTCAAGTG TGTGGGCGAG 
GCAGCGGGCG GCTTCTACTA CAAGGACCAC CTCCCAAGGC TCGGCAAGCC GGCGGAGCTG 
CAGCGCAGGG TGAGTGCTGA CAGCCAGCCC TTCCAGCACG GGGACAAGGT CAAGTGTCTG 
CTGGACACTG ATGTCCTGCG GGAGATGCAG GAAGGCCACG GCGGCTGGAA CCCCAGGATG 
GCGGAGTTTA TCGGACAGAC GGGCACCGTG CATCGTATCA CGGACCGCGG GGACGTGCGC 
GTGCAGTTCA ACCACGAGAC GCGCTGGACC TTCCACCCCG GGGCGCTCAC CAAGGACACC 
AGGAAGGCAG GACAGCTTCG TGGGCGGGAG GGAGGCGGCT GGGCTAAGAT GCTCCTGCAC 
CACTCCTTCT GGGTGGGCGA CGTGGTCCGG GTCATCGGCG ACCTTGACAC AGTGAAGCGG 
CTGCAGGCTG GGCATGGCGA GTGGACGGAC GACATGGCCC CTGCCCTGGG CCGCGTCGGG 
AAGGTGGTGA AAGTGTTTGG AGACGGGAAC CTGCGTGTAG CAGTCGCTGG TCAGCGGTGG 
ACCTTCAGCC CCTCCTGCCT GGTGGCCTAC CGGCCCGAGG AGGATGCCAA CCTGGACGTG 
GCCGAGCGCG CCCGGGAGAA CAAAAGCTCA CTGAGCGTGG CCCTGGACAA GCTTCGGGCC 
CAGAAGAGTG ACCCAGAGCA CCCGGGAAGG CTGGTGGTGG AGGTGGCGCT GGGTAACGCA 
GCCCGGGCTC TGGACCTGCT GCGGAGGCGC CCAGAGCAGG TGGACACCAA GAACCAAGGC 
AGGACCGCTC TGCAAGTGGC TGCCTACCTG GGCCAGGTGG AGTTGATACG GCTGCTGCTA 
CAAGCCAGGG CGGGCGTGGA CCTGCCGGAC GACGAGGGCA ACACGGCACT GCACTACGCG 
GCCCTGGGGA ACCAGCCCGA GGCCACCAGG GTGCTCCTGA GTGCTGGGTG CCGGGCGGAC 
GCCATCAACA GCACCCAGAG CACAGCACTG CACGTGGCCG TGCAGAGGGG CTTCCTGGAG 
GTGGTGCGGG CCCTGTGTGA GCGCGGCTGT GACGTCAACC TGCCCGACGC CCACTCGGAC 
ACGCCCCTGC ACTCCGCCAT CTCGGCGGGC ACTGGAGCCA GCGGCATTGT CGAGGTCCTC 
ACGGAGGTGC CAAACATCGA TGTTACCGCC ACCAACAGCC AGGGTTTCAC CCTGCTGCAC 
CATGCCTCCC TCAAGGGTCA CGCGCTAGCT GTGAGAAAGA TTCTGGCTCG GGCGCGGCAG 
CTGGTGGACG CCAAGAAGGA GGACGGCTTC ACGGCGCTGC ATCTGGCTGC CCTCAACAAC 
CACCGCGAGG TGGCCCAGAT CCTCATCCGG GAGGGCCGCT GTGACGTGAA CGTGCGCAAC 
CGGAAGCTGC AGTCCCCGCT GCATCTCGCC GTGCAACAGG CCCACGTGGG GCTGGTGCCG 
CTACTGGTGG ACGCTGGGTG CAGTGTCAAC GCCGAGGACG AGGAGGGGGA CACAGCCCTG 
CACGTGGCGC TGCAGCGTCA TCAGCTGCTG CCCCTGGTGG CTGATGGGGC CGGGGGGGAC 
CCAGGGCCCT TGCAGCTGCT GTCCAGGACA TGGGGCGCCG GCTGCTGGGG CTGCCAGGTG 
CCAGGAGACG CCTCCCTCGG GCCTGCCCCG GCGCCCGCCC TCACCGGCGT CTGTCCTGCC 
GCCCAGCTAC AGGCCTCGGG CCTCCCCGGC AGCGCGGAGC TGACGGTGGG CGCGGCGGTC 
GCCTGCTTCC TGGCGCTGGA GGGCGCCGAC GTGAGCTACA CCAACCACCG CGGTCGGAGC 
CCGCTGGACC TGGCCGCCGA GGGTCGCGTG CTCAAGGCCC TTCAGGGCTG CGCCCAGCGC 
TTCCGGGAGC GGCAGGCGGG CGGGGGCGCG GCCCCGGGCC CCAGGCAAAC GCTCGGGACC 
CCCAACACCG TGACGAACCT GCACGTGGGC GCCGCGCCGG GGCCCGAGGC CGCTGAGTGC 
CTGGTGTGCT CCGAGCTGGC GCTGCTGGTG CTGTTCTCGC CGTGCCAGCA CCGCACCGTG 
TGTGAGGGTG AGTGGGGGGC CCCGGGGTGG GGAGGCCCGG CTAGTAGGGC CGCAGCCAAC 
CGCGCTCTCC TCTTCGCAGA GTGCGCGCGC AGGATGAAGA AGTGCATCAG GTGCCAGGTG 
GTCGTCAGCA AGAAACTGCG CCCAGACGGC TCTGAGGTGG CGAGCGCCGC CCCCGCCCCC 
GGCCCGCCGC GCCAGCTGGT GGAGGAGCTG CAGAGCCGCT ACCGGCAGAT GGAGGAACGC 
ATCACCTGCC CCATCTGCAT CGACAGCCAC ATCCGCCTCG TGTTCCAGTG CGGCCACGGC 
GCATGCGCCC CCTGCGGCTC CGCGCTCAGC GCCTGCCCCA TCTGCCGCCA GCCCATCCGC 
GACCGCATCC AGATCTTCGT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000363.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855852.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OHu83172
Clone ID Related Accession (Same CDS sequence)	XM_017000363.1
Accession Version	XM_017000363.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCCAG ACCCCCAGGC GGGCGTGCAG GTGGGCATGC GGGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTTGGC 
CGCCACGGCA GCCCCTCGAC ACCCGACCGC ACAGTGGTCG TGCAGTGGGA CCAGGGCACG 
CGCACCAACT ACCGCGCCGG CTACCAGGGC GCGCACGACC TGCTGCTGTA CGACAACGCC 
CAGATCGGCG TCCGGCACCC CAACATCATC TGTGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGGATGCGCT GGAAGTGCCG TGTGTGCCTG GACTACGACC TCTGCACGCA GTGCTACATG 
CACAACAAGC ATGAGCTCGC CCACGCCTTC GACCGCTACG AGACCGCTCA CTCGCGCCCT 
GTCACACTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAC TAAGGGGCAT CTTCCAGGGA 
GCGAAGGTGG TGCGAGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG GGAAGGGAAA 
CCGGGCCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGGAG TGTGGCCAGC 
GTGACGTGGG CTGATGGTAC CACCAATGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCGGGCGGC TTCTACTACA AGGACCACCT CCCAAGGCTC 
GGCAAGCCGG CGGAGCTGCA GCGCAGGGTG AGTGCTGACA GCCAGCCCTT CCAGCACGGG 
GACAAGGTCA AGTGTCTGCT GGACACTGAT GTCCTGCGGG AGATGCAGGA AGGCCACGGC 
GGCTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA TCGTATCACG 
GACCGCGGGG ACGTGCGCGT GCAGTTCAAC CACGAGACGC GCTGGACCTT CCACCCCGGG 
GCGCTCACCA AGGACACCAG GAAGGCAGGA CAGCTTCGTG GGCGGGAGGG AGGCGGCTGG 
GCTAAGATGC TCCTGCACCA CTCCTTCTGG GTGGGCGACG TGGTCCGGGT CATCGGCGAC 
CTTGACACAG TGAAGCGGCT GCAGGCTGGG CATGGCGAGT GGACGGACGA CATGGCCCCT 
GCCCTGGGCC GCGTCGGGAA GGTGGTGAAA GTGTTTGGAG ACGGGAACCT GCGTGTAGCA 
GTCGCTGGTC AGCGGTGGAC CTTCAGCCCC TCCTGCCTGG TGGCCTACCG GCCCGAGGAG 
GATGCCAACC TGGACGTGGC CGAGCGCGCC CGGGAGAACA AAAGCTCACT GAGCGTGGCC 
CTGGACAAGC TTCGGGCCCA GAAGAGTGAC CCAGAGCACC CGGGAAGGCT GGTGGTGGAG 
GTGGCGCTGG GTAACGCAGC CCGGGCTCTG GACCTGCTGC GGAGGCGCCC AGAGCAGGTG 
GACACCAAGA ACCAAGGCAG GACCGCTCTG CAAGTGGCTG CCTACCTGGG CCAGGTGGAG 
TTGATACGGC TGCTGCTACA AGCCAGGGCG GGCGTGGACC TGCCGGACGA CGAGGGCAAC 
ACGGCACTGC ACTACGCGGC CCTGGGGAAC CAGCCCGAGG CCACCAGGGT GCTCCTGAGT 
GCTGGGTGCC GGGCGGACGC CATCAACAGC ACCCAGAGCA CAGCACTGCA CGTGGCCGTG 
CAGAGGGGCT TCCTGGAGGT GGTGCGGGCC CTGTGTGAGC GCGGCTGTGA CGTCAACCTG 
CCCGACGCCC ACTCGGACAC GCCCCTGCAC TCCGCCATCT CGGCGGGCAC TGGAGCCAGC 
GGCATTGTCG AGGTCCTCAC GGAGGTGCCA AACATCGATG TTACCGCCAC CAACAGCCAG 
GGTTTCACCC TGCTGCACCA TGCCTCCCTC AAGGGTCACG CGCTAGCTGT GAGAAAGATT 
CTGGCTCGGG CGCGGCAGCT GGTGGACGCC AAGAAGGAGG ACGGCTTCAC GGCGCTGCAT 
CTGGCTGCCC TCAACAACCA CCGCGAGGTG GCCCAGATCC TCATCCGGGA GGGCCGCTGT 
GACGTGAACG TGCGCAACCG GAAGCTGCAG TCCCCGCTGC ATCTCGCCGT GCAACAGGCC 
CACGTGGGGC TGGTGCCGCT ACTGGTGGAC GCTGGGTGCA GTGTCAACGC CGAGGACGAG 
GAGGGGGACA CAGCCCTGCA CGTGGCGCTG CAGCGTCATC AGCTGCTGCC CCTGGTGGCT 
GATGGGGCCG GGGGGGACCC AGGGCCCTTG CAGCTGCTGT CCAGGACATG GGGCGCCGGC 
TGCTGGGGCT GCCAGGTGCC AGGAGACGCC TCCCTCGGGC CTGCCCCGGC GCCCGCCCTC 
ACCGGCGTCT GTCCTGCCGC CCAGCTACAG GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG 
ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG GCGCTGGAGG GCGCCGACGT GAGCTACACC 
AACCACCGCG GTCGGAGCCC GCTGGACCTG GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT 
CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC 
AGGCAAACGC TCGGGACCCC CAACACCGTG ACGAACCTGC ACGTGGGCGC CGCGCCGGGG 
CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG 
TGCCAGCACC GCACCGTGTG TGAGGGTGAG TGGGGGGCCC CGGGGTGGGG AGGCCCGGCT 
AGTAGGGCCG CAGCCAACCG CGCTCTCCTC TTCGCAGAGT GCGCGCGCAG GATGAAGAAG 
TGCATCAGGT GCCAGGTGGT CGTCAGCAAG AAACTGCGCC CAGACGGCTC TGAGGTGGCG 
AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC 
CGGCAGATGG AGGAACGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG 
TTCCAGTGCG GCCACGGCGC ATGCGCCCCC TGCGGCTCCG CGCTCAGCGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855852.1
CDS	366..3467
Translation	MDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000363.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000363.1

ORF Insert Sequence:

ATGGACCCAG ACCCCCAGGC GGGCGTGCAG GTGGGCATGC GGGTGGTGCG CGGCGTGGAC 
TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTTGGC 
CGCCACGGCA GCCCCTCGAC ACCCGACCGC ACAGTGGTCG TGCAGTGGGA CCAGGGCACG 
CGCACCAACT ACCGCGCCGG CTACCAGGGC GCGCACGACC TGCTGCTGTA CGACAACGCC 
CAGATCGGCG TCCGGCACCC CAACATCATC TGTGACTGCT GCAAGAAGCA CGGGCTGCGG 
GGGATGCGCT GGAAGTGCCG TGTGTGCCTG GACTACGACC TCTGCACGCA GTGCTACATG 
CACAACAAGC ATGAGCTCGC CCACGCCTTC GACCGCTACG AGACCGCTCA CTCGCGCCCT 
GTCACACTGA GTCCCCGCCA GGGCCTCCCG AGGATCCCAC TAAGGGGCAT CTTCCAGGGA 
GCGAAGGTGG TGCGAGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG GGAAGGGAAA 
CCGGGCCGTG TGGTGGACAT CCGTGGCTGG GATGTGGAGA CAGGCCGGAG TGTGGCCAGC 
GTGACGTGGG CTGATGGTAC CACCAATGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAC 
CTCAAGTGTG TGGGCGAGGC AGCGGGCGGC TTCTACTACA AGGACCACCT CCCAAGGCTC 
GGCAAGCCGG CGGAGCTGCA GCGCAGGGTG AGTGCTGACA GCCAGCCCTT CCAGCACGGG 
GACAAGGTCA AGTGTCTGCT GGACACTGAT GTCCTGCGGG AGATGCAGGA AGGCCACGGC 
GGCTGGAACC CCAGGATGGC GGAGTTTATC GGACAGACGG GCACCGTGCA TCGTATCACG 
GACCGCGGGG ACGTGCGCGT GCAGTTCAAC CACGAGACGC GCTGGACCTT CCACCCCGGG 
GCGCTCACCA AGGACACCAG GAAGGCAGGA CAGCTTCGTG GGCGGGAGGG AGGCGGCTGG 
GCTAAGATGC TCCTGCACCA CTCCTTCTGG GTGGGCGACG TGGTCCGGGT CATCGGCGAC 
CTTGACACAG TGAAGCGGCT GCAGGCTGGG CATGGCGAGT GGACGGACGA CATGGCCCCT 
GCCCTGGGCC GCGTCGGGAA GGTGGTGAAA GTGTTTGGAG ACGGGAACCT GCGTGTAGCA 
GTCGCTGGTC AGCGGTGGAC CTTCAGCCCC TCCTGCCTGG TGGCCTACCG GCCCGAGGAG 
GATGCCAACC TGGACGTGGC CGAGCGCGCC CGGGAGAACA AAAGCTCACT GAGCGTGGCC 
CTGGACAAGC TTCGGGCCCA GAAGAGTGAC CCAGAGCACC CGGGAAGGCT GGTGGTGGAG 
GTGGCGCTGG GTAACGCAGC CCGGGCTCTG GACCTGCTGC GGAGGCGCCC AGAGCAGGTG 
GACACCAAGA ACCAAGGCAG GACCGCTCTG CAAGTGGCTG CCTACCTGGG CCAGGTGGAG 
TTGATACGGC TGCTGCTACA AGCCAGGGCG GGCGTGGACC TGCCGGACGA CGAGGGCAAC 
ACGGCACTGC ACTACGCGGC CCTGGGGAAC CAGCCCGAGG CCACCAGGGT GCTCCTGAGT 
GCTGGGTGCC GGGCGGACGC CATCAACAGC ACCCAGAGCA CAGCACTGCA CGTGGCCGTG 
CAGAGGGGCT TCCTGGAGGT GGTGCGGGCC CTGTGTGAGC GCGGCTGTGA CGTCAACCTG 
CCCGACGCCC ACTCGGACAC GCCCCTGCAC TCCGCCATCT CGGCGGGCAC TGGAGCCAGC 
GGCATTGTCG AGGTCCTCAC GGAGGTGCCA AACATCGATG TTACCGCCAC CAACAGCCAG 
GGTTTCACCC TGCTGCACCA TGCCTCCCTC AAGGGTCACG CGCTAGCTGT GAGAAAGATT 
CTGGCTCGGG CGCGGCAGCT GGTGGACGCC AAGAAGGAGG ACGGCTTCAC GGCGCTGCAT 
CTGGCTGCCC TCAACAACCA CCGCGAGGTG GCCCAGATCC TCATCCGGGA GGGCCGCTGT 
GACGTGAACG TGCGCAACCG GAAGCTGCAG TCCCCGCTGC ATCTCGCCGT GCAACAGGCC 
CACGTGGGGC TGGTGCCGCT ACTGGTGGAC GCTGGGTGCA GTGTCAACGC CGAGGACGAG 
GAGGGGGACA CAGCCCTGCA CGTGGCGCTG CAGCGTCATC AGCTGCTGCC CCTGGTGGCT 
GATGGGGCCG GGGGGGACCC AGGGCCCTTG CAGCTGCTGT CCAGGACATG GGGCGCCGGC 
TGCTGGGGCT GCCAGGTGCC AGGAGACGCC TCCCTCGGGC CTGCCCCGGC GCCCGCCCTC 
ACCGGCGTCT GTCCTGCCGC CCAGCTACAG GCCTCGGGCC TCCCCGGCAG CGCGGAGCTG 
ACGGTGGGCG CGGCGGTCGC CTGCTTCCTG GCGCTGGAGG GCGCCGACGT GAGCTACACC 
AACCACCGCG GTCGGAGCCC GCTGGACCTG GCCGCCGAGG GTCGCGTGCT CAAGGCCCTT 
CAGGGCTGCG CCCAGCGCTT CCGGGAGCGG CAGGCGGGCG GGGGCGCGGC CCCGGGCCCC 
AGGCAAACGC TCGGGACCCC CAACACCGTG ACGAACCTGC ACGTGGGCGC CGCGCCGGGG 
CCCGAGGCCG CTGAGTGCCT GGTGTGCTCC GAGCTGGCGC TGCTGGTGCT GTTCTCGCCG 
TGCCAGCACC GCACCGTGTG TGAGGGTGAG TGGGGGGCCC CGGGGTGGGG AGGCCCGGCT 
AGTAGGGCCG CAGCCAACCG CGCTCTCCTC TTCGCAGAGT GCGCGCGCAG GATGAAGAAG 
TGCATCAGGT GCCAGGTGGT CGTCAGCAAG AAACTGCGCC CAGACGGCTC TGAGGTGGCG 
AGCGCCGCCC CCGCCCCCGG CCCGCCGCGC CAGCTGGTGG AGGAGCTGCA GAGCCGCTAC 
CGGCAGATGG AGGAACGCAT CACCTGCCCC ATCTGCATCG ACAGCCACAT CCGCCTCGTG 
TTCCAGTGCG GCCACGGCGC ATGCGCCCCC TGCGGCTCCG CGCTCAGCGC CTGCCCCATC 
TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000364.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855853.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OHu83173
Clone ID Related Accession (Same CDS sequence)	XM_017000364.1
Accession Version	XM_017000364.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGCAAGCCGG CGGAGCTGCA GCGCAGGGTG 
AGTGCTGACA GCCAGCCCTT CCAGCACGGG GACAAGGTCA AGTGTCTGCT GGACACTGAT 
GTCCTGCGGG AGATGCAGGA AGGCCACGGC GGCTGGAACC CCAGGATGGC GGAGTTTATC 
GGACAGACGG GCACCGTGCA TCGTATCACG GACCGCGGGG ACGTGCGCGT GCAGTTCAAC 
CACGAGACGC GCTGGACCTT CCACCCCGGG GCGCTCACCA AGCACCACTC CTTCTGGGTG 
GGCGACGTGG TCCGGGTCAT CGGCGACCTT GACACAGTGA AGCGGCTGCA GGCTGGGCAT 
GGCGAGTGGA CGGACGACAT GGCCCCTGCC CTGGGCCGCG TCGGGAAGGT GGTGAAAGTG 
TTTGGAGACG GGAACCTGCG TGTAGCAGTC GCTGGTCAGC GGTGGACCTT CAGCCCCTCC 
TGCCTGGTGG CCTACCGGCC CGAGGAGGAT GCCAACCTGG ACGTGGCCGA GCGCGCCCGG 
GAGAACAAAA GCTCACTGAG CGTGGCCCTG GACAAGCTTC GGGCCCAGAA GAGTGACCCA 
GAGCACCCGG GAAGGCTGGT GGTGGAGGTG GCGCTGGGTA ACGCAGCCCG GGCTCTGGAC 
CTGCTGCGGA GGCGCCCAGA GCAGGTGGAC ACCAAGAACC AAGGCAGGAC CGCTCTGCAA 
GTGGCTGCCT ACCTGGGCCA GGTGGAGTTG ATACGGCTGC TGCTACAAGC CAGGGCGGGC 
GTGGACCTGC CGGACGACGA GGGCAACACG GCACTGCACT ACGCGGCCCT GGGGAACCAG 
CCCGAGGCCA CCAGGGTGCT CCTGAGTGCT GGGTGCCGGG CGGACGCCAT CAACAGCACC 
CAGAGCACAG CACTGCACGT GGCCGTGCAG AGGGGCTTCC TGGAGGTGGT GCGGGCCCTG 
TGTGAGCGCG GCTGTGACGT CAACCTGCCC GACGCCCACT CGGACACGCC CCTGCACTCC 
GCCATCTCGG CGGGCACTGG AGCCAGCGGC ATTGTCGAGG TCCTCACGGA GGTGCCAAAC 
ATCGATGTTA CCGCCACCAA CAGCCAGGGT TTCACCCTGC TGCACCATGC CTCCCTCAAG 
GGTCACGCGC TAGCTGTGAG AAAGATTCTG GCTCGGGCGC GGCAGCTGGT GGACGCCAAG 
AAGGAGGACG GCTTCACGGC GCTGCATCTG GCTGCCCTCA ACAACCACCG CGAGGTGGCC 
CAGATCCTCA TCCGGGAGGG CCGCTGTGAC GTGAACGTGC GCAACCGGAA GCTGCAGTCC 
CCGCTGCATC TCGCCGTGCA ACAGGCCCAC GTGGGGCTGG TGCCGCTACT GGTGGACGCT 
GGGTGCAGTG TCAACGCCGA GGACGAGGAG GGGGACACAG CCCTGCACGT GGCGCTGCAG 
CGTCATCAGC TGCTGCCCCT GGTGGCTGAT GGGGCCGGGG GGGACCCAGG GCCCTTGCAG 
CTGCTGTCCA GGCTACAGGC CTCGGGCCTC CCCGGCAGCG CGGAGCTGAC GGTGGGCGCG 
GCGGTCGCCT GCTTCCTGGC GCTGGAGGGC GCCGACGTGA GCTACACCAA CCACCGCGGT 
CGGAGCCCGC TGGACCTGGC CGCCGAGGGT CGCGTGCTCA AGGCCCTTCA GGGCTGCGCC 
CAGCGCTTCC GGGAGCGGCA GGCGGGCGGG GGCGCGGCCC CGGGCCCCAG GCAAACGCTC 
GGGACCCCCA ACACCGTGAC GAACCTGCAC GTGGGCGCCG CGCCGGGGCC CGAGGCCGCT 
GAGTGCCTGG TGTGCTCCGA GCTGGCGCTG CTGGTGCTGT TCTCGCCGTG CCAGCACCGC 
ACCGTGTGTG AGGGTGAGTG GGGGGCCCCG GGGTGGGGAG GCCCGGCTAG TAGGGCCGCA 
GCCAACCGCG CTCTCCTCTT CGCAGAGTGC GCGCGCAGGA TGAAGAAGTG CATCAGGTGC 
CAGGTGGTCG TCAGCAAGAA ACTGCGCCCA GACGGCTCTG AGGTGGCGAG CGCCGCCCCC 
GCCCCCGGCC CGCCGCGCCA GCTGGTGGAG GAGCTGCAGA GCCGCTACCG GCAGATGGAG 
GAACGCATCA CCTGCCCCAT CTGCATCGAC AGCCACATCC GCCTCGTGTT CCAGTGCGGC 
CACGGCGCAT GCGCCCCCTG CGGCTCCGCG CTCAGCGCCT GCCCCATCTG CCGCCAGCCC 
ATCCGCGACC GCATCCAGAT CTTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855853.1
CDS	18..3107
Translation	MAGALRRGRALGSRPSGPTVSSRRSPQCPVAQEGLGARSRPRVAPRSLARCGPSSRLMGWKPSEARGQSQSFQASGLQPRSLKAARRATGRPDRSRAARPTMDPSAHRSRAAPPNMDPDPQAGVQVGMRVVRGVDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQGAHDLLLYDNAQIGVRHPNIICDCCKKHGLRGMRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000364.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000364.1

ORF Insert Sequence:

ATGGCGGGGG CGCTCCGGCG GGGGCGGGCC CTGGGCTCCC GCCCTTCGGG TCCCACAGTT 
TCCAGCCGCC GCTCTCCTCA GTGCCCGGTG GCCCAGGAGG GCCTGGGAGC CCGAAGCCGT 
CCCCGAGTCG CTCCTAGGTC ACTGGCGCGA TGCGGGCCGT CCTCTCGGCT GATGGGTTGG 
AAGCCCAGCG AGGCTAGAGG CCAGTCCCAA AGTTTCCAGG CATCAGGGCT GCAGCCCAGG 
AGCCTCAAGG CGGCCCGGCG GGCGACTGGA CGGCCGGACA GGTCCCGAGC AGCCCGGCCC 
ACCATGGACC CCTCTGCCCA CAGGTCCCGA GCAGCCCCGC CCAACATGGA CCCAGACCCC 
CAGGCGGGCG TGCAGGTGGG CATGCGGGTG GTGCGCGGCG TGGACTGGAA GTGGGGCCAG 
CAGGACGGCG GCGAGGGCGG CGTGGGCACG GTGGTGGAGC TTGGCCGCCA CGGCAGCCCC 
TCGACACCCG ACCGCACAGT GGTCGTGCAG TGGGACCAGG GCACGCGCAC CAACTACCGC 
GCCGGCTACC AGGGCGCGCA CGACCTGCTG CTGTACGACA ACGCCCAGAT CGGCGTCCGG 
CACCCCAACA TCATCTGTGA CTGCTGCAAG AAGCACGGGC TGCGGGGGAT GCGCTGGAAG 
TGCCGTGTGT GCCTGGACTA CGACCTCTGC ACGCAGTGCT ACATGCACAA CAAGCATGAG 
CTCGCCCACG CCTTCGACCG CTACGAGACC GCTCACTCGC GCCCTGTCAC ACTGAGTCCC 
CGCCAGGGCC TCCCGAGGAT CCCACTAAGG GGCATCTTCC AGGGAGCGAA GGTGGTGCGA 
GGCCCCGACT GGGAGTGGGG CTCACAGGAT GGCAAGCCGG CGGAGCTGCA GCGCAGGGTG 
AGTGCTGACA GCCAGCCCTT CCAGCACGGG GACAAGGTCA AGTGTCTGCT GGACACTGAT 
GTCCTGCGGG AGATGCAGGA AGGCCACGGC GGCTGGAACC CCAGGATGGC GGAGTTTATC 
GGACAGACGG GCACCGTGCA TCGTATCACG GACCGCGGGG ACGTGCGCGT GCAGTTCAAC 
CACGAGACGC GCTGGACCTT CCACCCCGGG GCGCTCACCA AGCACCACTC CTTCTGGGTG 
GGCGACGTGG TCCGGGTCAT CGGCGACCTT GACACAGTGA AGCGGCTGCA GGCTGGGCAT 
GGCGAGTGGA CGGACGACAT GGCCCCTGCC CTGGGCCGCG TCGGGAAGGT GGTGAAAGTG 
TTTGGAGACG GGAACCTGCG TGTAGCAGTC GCTGGTCAGC GGTGGACCTT CAGCCCCTCC 
TGCCTGGTGG CCTACCGGCC CGAGGAGGAT GCCAACCTGG ACGTGGCCGA GCGCGCCCGG 
GAGAACAAAA GCTCACTGAG CGTGGCCCTG GACAAGCTTC GGGCCCAGAA GAGTGACCCA 
GAGCACCCGG GAAGGCTGGT GGTGGAGGTG GCGCTGGGTA ACGCAGCCCG GGCTCTGGAC 
CTGCTGCGGA GGCGCCCAGA GCAGGTGGAC ACCAAGAACC AAGGCAGGAC CGCTCTGCAA 
GTGGCTGCCT ACCTGGGCCA GGTGGAGTTG ATACGGCTGC TGCTACAAGC CAGGGCGGGC 
GTGGACCTGC CGGACGACGA GGGCAACACG GCACTGCACT ACGCGGCCCT GGGGAACCAG 
CCCGAGGCCA CCAGGGTGCT CCTGAGTGCT GGGTGCCGGG CGGACGCCAT CAACAGCACC 
CAGAGCACAG CACTGCACGT GGCCGTGCAG AGGGGCTTCC TGGAGGTGGT GCGGGCCCTG 
TGTGAGCGCG GCTGTGACGT CAACCTGCCC GACGCCCACT CGGACACGCC CCTGCACTCC 
GCCATCTCGG CGGGCACTGG AGCCAGCGGC ATTGTCGAGG TCCTCACGGA GGTGCCAAAC 
ATCGATGTTA CCGCCACCAA CAGCCAGGGT TTCACCCTGC TGCACCATGC CTCCCTCAAG 
GGTCACGCGC TAGCTGTGAG AAAGATTCTG GCTCGGGCGC GGCAGCTGGT GGACGCCAAG 
AAGGAGGACG GCTTCACGGC GCTGCATCTG GCTGCCCTCA ACAACCACCG CGAGGTGGCC 
CAGATCCTCA TCCGGGAGGG CCGCTGTGAC GTGAACGTGC GCAACCGGAA GCTGCAGTCC 
CCGCTGCATC TCGCCGTGCA ACAGGCCCAC GTGGGGCTGG TGCCGCTACT GGTGGACGCT 
GGGTGCAGTG TCAACGCCGA GGACGAGGAG GGGGACACAG CCCTGCACGT GGCGCTGCAG 
CGTCATCAGC TGCTGCCCCT GGTGGCTGAT GGGGCCGGGG GGGACCCAGG GCCCTTGCAG 
CTGCTGTCCA GGCTACAGGC CTCGGGCCTC CCCGGCAGCG CGGAGCTGAC GGTGGGCGCG 
GCGGTCGCCT GCTTCCTGGC GCTGGAGGGC GCCGACGTGA GCTACACCAA CCACCGCGGT 
CGGAGCCCGC TGGACCTGGC CGCCGAGGGT CGCGTGCTCA AGGCCCTTCA GGGCTGCGCC 
CAGCGCTTCC GGGAGCGGCA GGCGGGCGGG GGCGCGGCCC CGGGCCCCAG GCAAACGCTC 
GGGACCCCCA ACACCGTGAC GAACCTGCAC GTGGGCGCCG CGCCGGGGCC CGAGGCCGCT 
GAGTGCCTGG TGTGCTCCGA GCTGGCGCTG CTGGTGCTGT TCTCGCCGTG CCAGCACCGC 
ACCGTGTGTG AGGGTGAGTG GGGGGCCCCG GGGTGGGGAG GCCCGGCTAG TAGGGCCGCA 
GCCAACCGCG CTCTCCTCTT CGCAGAGTGC GCGCGCAGGA TGAAGAAGTG CATCAGGTGC 
CAGGTGGTCG TCAGCAAGAA ACTGCGCCCA GACGGCTCTG AGGTGGCGAG CGCCGCCCCC 
GCCCCCGGCC CGCCGCGCCA GCTGGTGGAG GAGCTGCAGA GCCGCTACCG GCAGATGGAG 
GAACGCATCA CCTGCCCCAT CTGCATCGAC AGCCACATCC GCCTCGTGTT CCAGTGCGGC 
CACGGCGCAT GCGCCCCCTG CGGCTCCGCG CTCAGCGCCT GCCCCATCTG CCGCCAGCCC 
ATCCGCGACC GCATCCAGAT CTTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000365.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855854.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OHu83174
Clone ID Related Accession (Same CDS sequence)	XM_017000365.1
Accession Version	XM_017000365.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2799bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTGGA AGTGCCGTGT GTGCCTGGAC TACGACCTCT GCACGCAGTG CTACATGCAC 
AACAAGCATG AGCTCGCCCA CGCCTTCGAC CGCTACGAGA CCGCTCACTC GCGCCCTGTC 
ACACTGAGTC CCCGCCAGGG CCTCCCGAGG ATCCCACTAA GGGGCATCTT CCAGGGAGCG 
AAGGTGGTGC GAGGCCCCGA CTGGGAGTGG GGCTCACAGG ATGGAGGGGA AGGGAAACCG 
GGCCGTGTGG TGGACATCCG TGGCTGGGAT GTGGAGACAG GCCGGAGTGT GGCCAGCGTG 
ACGTGGGCTG ATGGTACCAC CAATGTGTAC CGTGTGGGCC ACAAGGGCAA GGTGGACCTC 
AAGTGTGTGG GCGAGGCAGC GGGCGGCTTC TACTACAAGG ACCACCTCCC AAGGCTCGGC 
AAGCCGGCGG AGCTGCAGCG CAGGGTGAGT GCTGACAGCC AGCCCTTCCA GCACGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACTGATGTC CTGCGGGAGA TGCAGGAAGG CCACGGCGGC 
TGGAACCCCA GGATGGCGGA GTTTATCGGA CAGACGGGCA CCGTGCATCG TATCACGGAC 
CGCGGGGACG TGCGCGTGCA GTTCAACCAC GAGACGCGCT GGACCTTCCA CCCCGGGGCG 
CTCACCAAGG ACACCAGGAA GGCAGGACAG CTTCGTGGGC GGGAGGGAGG CGGCTGGGCT 
AAGATGCTCC TGCACCACTC CTTCTGGGTG GGCGACGTGG TCCGGGTCAT CGGCGACCTT 
GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CGGACGACAT GGCCCCTGCC 
CTGGGCCGCG TCGGGAAGGT GGTGAAAGTG TTTGGAGACG GGAACCTGCG TGTAGCAGTC 
GCTGGTCAGC GGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC CGAGGAGGAT 
GCCAACCTGG ACGTGGCCGA GCGCGCCCGG GAGAACAAAA GCTCACTGAG CGTGGCCCTG 
GACAAGCTTC GGGCCCAGAA GAGTGACCCA GAGCACCCGG GAAGGCTGGT GGTGGAGGTG 
GCGCTGGGTA ACGCAGCCCG GGCTCTGGAC CTGCTGCGGA GGCGCCCAGA GCAGGTGGAC 
ACCAAGAACC AAGGCAGGAC CGCTCTGCAA GTGGCTGCCT ACCTGGGCCA GGTGGAGTTG 
ATACGGCTGC TGCTACAAGC CAGGGCGGGC GTGGACCTGC CGGACGACGA GGGCAACACG 
GCACTGCACT ACGCGGCCCT GGGGAACCAG CCCGAGGCCA CCAGGGTGCT CCTGAGTGCT 
GGGTGCCGGG CGGACGCCAT CAACAGCACC CAGAGCACAG CACTGCACGT GGCCGTGCAG 
AGGGGCTTCC TGGAGGTGGT GCGGGCCCTG TGTGAGCGCG GCTGTGACGT CAACCTGCCC 
GACGCCCACT CGGACACGCC CCTGCACTCC GCCATCTCGG CGGGCACTGG AGCCAGCGGC 
ATTGTCGAGG TCCTCACGGA GGTGCCAAAC ATCGATGTTA CCGCCACCAA CAGCCAGGGT 
TTCACCCTGC TGCACCATGC CTCCCTCAAG GGTCACGCGC TAGCTGTGAG AAAGATTCTG 
GCTCGGGCGC GGCAGCTGGT GGACGCCAAG AAGGAGGACG GCTTCACGGC GCTGCATCTG 
GCTGCCCTCA ACAACCACCG CGAGGTGGCC CAGATCCTCA TCCGGGAGGG CCGCTGTGAC 
GTGAACGTGC GCAACCGGAA GCTGCAGTCC CCGCTGCATC TCGCCGTGCA ACAGGCCCAC 
GTGGGGCTGG TGCCGCTACT GGTGGACGCT GGGTGCAGTG TCAACGCCGA GGACGAGGAG 
GGGGACACAG CCCTGCACGT GGCGCTGCAG CGTCATCAGC TGCTGCCCCT GGTGGCTGAT 
GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGACATGGGG CGCCGGCTGC 
TGGGGCTGCC AGGTGCCAGG AGACGCCTCC CTCGGGCCTG CCCCGGCGCC CGCCCTCACC 
GGCGTCTGTC CTGCCGCCCA GCTACAGGCC TCGGGCCTCC CCGGCAGCGC GGAGCTGACG 
GTGGGCGCGG CGGTCGCCTG CTTCCTGGCG CTGGAGGGCG CCGACGTGAG CTACACCAAC 
CACCGCGGTC GGAGCCCGCT GGACCTGGCC GCCGAGGGTC GCGTGCTCAA GGCCCTTCAG 
GGCTGCGCCC AGCGCTTCCG GGAGCGGCAG GCGGGCGGGG GCGCGGCCCC GGGCCCCAGG 
CAAACGCTCG GGACCCCCAA CACCGTGACG AACCTGCACG TGGGCGCCGC GCCGGGGCCC 
GAGGCCGCTG AGTGCCTGGT GTGCTCCGAG CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC 
CAGCACCGCA CCGTGTGTGA GGGTGAGTGG GGGGCCCCGG GGTGGGGAGG CCCGGCTAGT 
AGGGCCGCAG CCAACCGCGC TCTCCTCTTC GCAGAGTGCG CGCGCAGGAT GAAGAAGTGC 
ATCAGGTGCC AGGTGGTCGT CAGCAAGAAA CTGCGCCCAG ACGGCTCTGA GGTGGCGAGC 
GCCGCCCCCG CCCCCGGCCC GCCGCGCCAG CTGGTGGAGG AGCTGCAGAG CCGCTACCGG 
CAGATGGAGG AACGCATCAC CTGCCCCATC TGCATCGACA GCCACATCCG CCTCGTGTTC 
CAGTGCGGCC ACGGCGCATG CGCCCCCTGC GGCTCCGCGC TCAGCGCCTG CCCCATCTGC 
CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855854.1
CDS	396..3194
Translation	MRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKDTRKAGQLRGREGGGWAKMLLHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000365.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000365.1

ORF Insert Sequence:

ATGCGCTGGA AGTGCCGTGT GTGCCTGGAC TACGACCTCT GCACGCAGTG CTACATGCAC 
AACAAGCATG AGCTCGCCCA CGCCTTCGAC CGCTACGAGA CCGCTCACTC GCGCCCTGTC 
ACACTGAGTC CCCGCCAGGG CCTCCCGAGG ATCCCACTAA GGGGCATCTT CCAGGGAGCG 
AAGGTGGTGC GAGGCCCCGA CTGGGAGTGG GGCTCACAGG ATGGAGGGGA AGGGAAACCG 
GGCCGTGTGG TGGACATCCG TGGCTGGGAT GTGGAGACAG GCCGGAGTGT GGCCAGCGTG 
ACGTGGGCTG ATGGTACCAC CAATGTGTAC CGTGTGGGCC ACAAGGGCAA GGTGGACCTC 
AAGTGTGTGG GCGAGGCAGC GGGCGGCTTC TACTACAAGG ACCACCTCCC AAGGCTCGGC 
AAGCCGGCGG AGCTGCAGCG CAGGGTGAGT GCTGACAGCC AGCCCTTCCA GCACGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACTGATGTC CTGCGGGAGA TGCAGGAAGG CCACGGCGGC 
TGGAACCCCA GGATGGCGGA GTTTATCGGA CAGACGGGCA CCGTGCATCG TATCACGGAC 
CGCGGGGACG TGCGCGTGCA GTTCAACCAC GAGACGCGCT GGACCTTCCA CCCCGGGGCG 
CTCACCAAGG ACACCAGGAA GGCAGGACAG CTTCGTGGGC GGGAGGGAGG CGGCTGGGCT 
AAGATGCTCC TGCACCACTC CTTCTGGGTG GGCGACGTGG TCCGGGTCAT CGGCGACCTT 
GACACAGTGA AGCGGCTGCA GGCTGGGCAT GGCGAGTGGA CGGACGACAT GGCCCCTGCC 
CTGGGCCGCG TCGGGAAGGT GGTGAAAGTG TTTGGAGACG GGAACCTGCG TGTAGCAGTC 
GCTGGTCAGC GGTGGACCTT CAGCCCCTCC TGCCTGGTGG CCTACCGGCC CGAGGAGGAT 
GCCAACCTGG ACGTGGCCGA GCGCGCCCGG GAGAACAAAA GCTCACTGAG CGTGGCCCTG 
GACAAGCTTC GGGCCCAGAA GAGTGACCCA GAGCACCCGG GAAGGCTGGT GGTGGAGGTG 
GCGCTGGGTA ACGCAGCCCG GGCTCTGGAC CTGCTGCGGA GGCGCCCAGA GCAGGTGGAC 
ACCAAGAACC AAGGCAGGAC CGCTCTGCAA GTGGCTGCCT ACCTGGGCCA GGTGGAGTTG 
ATACGGCTGC TGCTACAAGC CAGGGCGGGC GTGGACCTGC CGGACGACGA GGGCAACACG 
GCACTGCACT ACGCGGCCCT GGGGAACCAG CCCGAGGCCA CCAGGGTGCT CCTGAGTGCT 
GGGTGCCGGG CGGACGCCAT CAACAGCACC CAGAGCACAG CACTGCACGT GGCCGTGCAG 
AGGGGCTTCC TGGAGGTGGT GCGGGCCCTG TGTGAGCGCG GCTGTGACGT CAACCTGCCC 
GACGCCCACT CGGACACGCC CCTGCACTCC GCCATCTCGG CGGGCACTGG AGCCAGCGGC 
ATTGTCGAGG TCCTCACGGA GGTGCCAAAC ATCGATGTTA CCGCCACCAA CAGCCAGGGT 
TTCACCCTGC TGCACCATGC CTCCCTCAAG GGTCACGCGC TAGCTGTGAG AAAGATTCTG 
GCTCGGGCGC GGCAGCTGGT GGACGCCAAG AAGGAGGACG GCTTCACGGC GCTGCATCTG 
GCTGCCCTCA ACAACCACCG CGAGGTGGCC CAGATCCTCA TCCGGGAGGG CCGCTGTGAC 
GTGAACGTGC GCAACCGGAA GCTGCAGTCC CCGCTGCATC TCGCCGTGCA ACAGGCCCAC 
GTGGGGCTGG TGCCGCTACT GGTGGACGCT GGGTGCAGTG TCAACGCCGA GGACGAGGAG 
GGGGACACAG CCCTGCACGT GGCGCTGCAG CGTCATCAGC TGCTGCCCCT GGTGGCTGAT 
GGGGCCGGGG GGGACCCAGG GCCCTTGCAG CTGCTGTCCA GGACATGGGG CGCCGGCTGC 
TGGGGCTGCC AGGTGCCAGG AGACGCCTCC CTCGGGCCTG CCCCGGCGCC CGCCCTCACC 
GGCGTCTGTC CTGCCGCCCA GCTACAGGCC TCGGGCCTCC CCGGCAGCGC GGAGCTGACG 
GTGGGCGCGG CGGTCGCCTG CTTCCTGGCG CTGGAGGGCG CCGACGTGAG CTACACCAAC 
CACCGCGGTC GGAGCCCGCT GGACCTGGCC GCCGAGGGTC GCGTGCTCAA GGCCCTTCAG 
GGCTGCGCCC AGCGCTTCCG GGAGCGGCAG GCGGGCGGGG GCGCGGCCCC GGGCCCCAGG 
CAAACGCTCG GGACCCCCAA CACCGTGACG AACCTGCACG TGGGCGCCGC GCCGGGGCCC 
GAGGCCGCTG AGTGCCTGGT GTGCTCCGAG CTGGCGCTGC TGGTGCTGTT CTCGCCGTGC 
CAGCACCGCA CCGTGTGTGA GGGTGAGTGG GGGGCCCCGG GGTGGGGAGG CCCGGCTAGT 
AGGGCCGCAG CCAACCGCGC TCTCCTCTTC GCAGAGTGCG CGCGCAGGAT GAAGAAGTGC 
ATCAGGTGCC AGGTGGTCGT CAGCAAGAAA CTGCGCCCAG ACGGCTCTGA GGTGGCGAGC 
GCCGCCCCCG CCCCCGGCCC GCCGCGCCAG CTGGTGGAGG AGCTGCAGAG CCGCTACCGG 
CAGATGGAGG AACGCATCAC CTGCCCCATC TGCATCGACA GCCACATCCG CCTCGTGTTC 
CAGTGCGGCC ACGGCGCATG CGCCCCCTGC GGCTCCGCGC TCAGCGCCTG CCCCATCTGC 
CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIB2 ( XM_017000366.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016855855.1 Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X27, mRNA.

Price & Availability↑

CloneID	OHu83175
Clone ID Related Accession (Same CDS sequence)	XM_017000366.1
Accession Version	XM_017000366.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	E3 ubiquitin-protein ligase MIB2 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCTGGA AGTGCCGTGT GTGCCTGGAC TACGACCTCT GCACGCAGTG CTACATGCAC 
AACAAGCATG AGCTCGCCCA CGCCTTCGAC CGCTACGAGA CCGCTCACTC GCGCCCTGTC 
ACACTGAGTC CCCGCCAGGG CCTCCCGAGG ATCCCACTAA GGGGCATCTT CCAGGGAGCG 
AAGGTGGTGC GAGGCCCCGA CTGGGAGTGG GGCTCACAGG ATGGAGGGGA AGGGAAACCG 
GGCCGTGTGG TGGACATCCG TGGCTGGGAT GTGGAGACAG GCCGGAGTGT GGCCAGCGTG 
ACGTGGGCTG ATGGTACCAC CAATGTGTAC CGTGTGGGCC ACAAGGGCAA GGTGGACCTC 
AAGTGTGTGG GCGAGGCAGC GGGCGGCTTC TACTACAAGG ACCACCTCCC AAGGCTCGGC 
AAGCCGGCGG AGCTGCAGCG CAGGGTGAGT GCTGACAGCC AGCCCTTCCA GCACGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACTGATGTC CTGCGGGAGA TGCAGGAAGG CCACGGCGGC 
TGGAACCCCA GGATGGCGGA GTTTATCGGA CAGACGGGCA CCGTGCATCG TATCACGGAC 
CGCGGGGACG TGCGCGTGCA GTTCAACCAC GAGACGCGCT GGACCTTCCA CCCCGGGGCG 
CTCACCAAGC ACCACTCCTT CTGGGTGGGC GACGTGGTCC GGGTCATCGG CGACCTTGAC 
ACAGTGAAGC GGCTGCAGGC TGGGCATGGC GAGTGGACGG ACGACATGGC CCCTGCCCTG 
GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT GGAGACGGGA ACCTGCGTGT AGCAGTCGCT 
GGTCAGCGGT GGACCTTCAG CCCCTCCTGC CTGGTGGCCT ACCGGCCCGA GGAGGATGCC 
AACCTGGACG TGGCCGAGCG CGCCCGGGAG AACAAAAGCT CACTGAGCGT GGCCCTGGAC 
AAGCTTCGGG CCCAGAAGAG TGACCCAGAG CACCCGGGAA GGCTGGTGGT GGAGGTGGCG 
CTGGGTAACG CAGCCCGGGC TCTGGACCTG CTGCGGAGGC GCCCAGAGCA GGTGGACACC 
AAGAACCAAG GCAGGACCGC TCTGCAAGTG GCTGCCTACC TGGGCCAGGT GGAGTTGATA 
CGGCTGCTGC TACAAGCCAG GGCGGGCGTG GACCTGCCGG ACGACGAGGG CAACACGGCA 
CTGCACTACG CGGCCCTGGG GAACCAGCCC GAGGCCACCA GGGTGCTCCT GAGTGCTGGG 
TGCCGGGCGG ACGCCATCAA CAGCACCCAG AGCACAGCAC TGCACGTGGC CGTGCAGAGG 
GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT GAGCGCGGCT GTGACGTCAA CCTGCCCGAC 
GCCCACTCGG ACACGCCCCT GCACTCCGCC ATCTCGGCGG GCACTGGAGC CAGCGGCATT 
GTCGAGGTCC TCACGGAGGT GCCAAACATC GATGTTACCG CCACCAACAG CCAGGGTTTC 
ACCCTGCTGC ACCATGCCTC CCTCAAGGGT CACGCGCTAG CTGTGAGAAA GATTCTGGCT 
CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG GAGGACGGCT TCACGGCGCT GCATCTGGCT 
GCCCTCAACA ACCACCGCGA GGTGGCCCAG ATCCTCATCC GGGAGGGCCG CTGTGACGTG 
AACGTGCGCA ACCGGAAGCT GCAGTCCCCG CTGCATCTCG CCGTGCAACA GGCCCACGTG 
GGGCTGGTGC CGCTACTGGT GGACGCTGGG TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG 
GACACAGCCC TGCACGTGGC GCTGCAGCGT CATCAGCTGC TGCCCCTGGT GGCTGATGGG 
GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG CTGTCCAGGA CATGGGGCGC CGGCTGCTGG 
GGCTGCCAGG TGCCAGGAGA CGCCTCCCTC GGGCCTGCCC CGGCGCCCGC CCTCACCGGC 
GTCTGTCCTG CCGCCCAGCT ACAGGCCTCG GGCCTCCCCG GCAGCGCGGA GCTGACGGTG 
GGCGCGGCGG TCGCCTGCTT CCTGGCGCTG GAGGGCGCCG ACGTGAGCTA CACCAACCAC 
CGCGGTCGGA GCCCGCTGGA CCTGGCCGCC GAGGGTCGCG TGCTCAAGGC CCTTCAGGGC 
TGCGCCCAGC GCTTCCGGGA GCGGCAGGCG GGCGGGGGCG CGGCCCCGGG CCCCAGGCAA 
ACGCTCGGGA CCCCCAACAC CGTGACGAAC CTGCACGTGG GCGCCGCGCC GGGGCCCGAG 
GCCGCTGAGT GCCTGGTGTG CTCCGAGCTG GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG 
CACCGCACCG TGTGTGAGGG TGAGTGGGGG GCCCCGGGGT GGGGAGGCCC GGCTAGTAGG 
GCCGCAGCCA ACCGCGCTCT CCTCTTCGCA GAGTGCGCGC GCAGGATGAA GAAGTGCATC 
AGGTGCCAGG TGGTCGTCAG CAAGAAACTG CGCCCAGACG GCTCTGAGGT GGCGAGCGCC 
GCCCCCGCCC CCGGCCCGCC GCGCCAGCTG GTGGAGGAGC TGCAGAGCCG CTACCGGCAG 
ATGGAGGAAC GCATCACCTG CCCCATCTGC ATCGACAGCC ACATCCGCCT CGTGTTCCAG 
TGCGGCCACG GCGCATGCGC CCCCTGCGGC TCCGCGCTCA GCGCCTGCCC CATCTGCCGC 
CAGCCCATCC GCGACCGCAT CCAGATCTTC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016855855.1
CDS	396..3131
Translation	MRWKCRVCLDYDLCTQCYMHNKHELAHAFDRYETAHSRPVTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKPGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCVGEAAGGFYYKDHLPRLGKPAELQRRVSADSQPFQHGDKVKCLLDTDVLREMQEGHGGWNPRMAEFIGQTGTVHRITDRGDVRVQFNHETRWTFHPGALTKHHSFWVGDVVRVIGDLDTVKRLQAGHGEWTDDMAPALGRVGKVVKVFGDGNLRVAVAGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLRAQKSDPEHPGRLVVEVALGNAARALDLLRRRPEQVDTKNQGRTALQVAAYLGQVELIRLLLQARAGVDLPDDEGNTALHYAALGNQPEATRVLLSAGCRADAINSTQSTALHVAVQRGFLEVVRALCERGCDVNLPDAHSDTPLHSAISAGTGASGIVEVLTEVPNIDVTATNSQGFTLLHHASLKGHALAVRKILARARQLVDAKKEDGFTALHLAALNNHREVAQILIREGRCDVNVRNRKLQSPLHLAVQQAHVGLVPLLVDAGCSVNAEDEEGDTALHVALQRHQLLPLVADGAGGDPGPLQLLSRTWGAGCWGCQVPGDASLGPAPAPALTGVCPAAQLQASGLPGSAELTVGAAVACFLALEGADVSYTNHRGRSPLDLAAEGRVLKALQGCAQRFRERQAGGGAAPGPRQTLGTPNTVTNLHVGAAPGPEAAECLVCSELALLVLFSPCQHRTVCEGEWGAPGWGGPASRAAANRALLFAECARRMKKCIRCQVVVSKKLRPDGSEVASAAPAPGPPRQLVEELQSRYRQMEERITCPICIDSHIRLVFQCGHGACAPCGSALSACPICRQPIRDRIQIFV

Target ORF information:

RefSeq Version	XM_017000366.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017000366.1

ORF Insert Sequence:

ATGCGCTGGA AGTGCCGTGT GTGCCTGGAC TACGACCTCT GCACGCAGTG CTACATGCAC 
AACAAGCATG AGCTCGCCCA CGCCTTCGAC CGCTACGAGA CCGCTCACTC GCGCCCTGTC 
ACACTGAGTC CCCGCCAGGG CCTCCCGAGG ATCCCACTAA GGGGCATCTT CCAGGGAGCG 
AAGGTGGTGC GAGGCCCCGA CTGGGAGTGG GGCTCACAGG ATGGAGGGGA AGGGAAACCG 
GGCCGTGTGG TGGACATCCG TGGCTGGGAT GTGGAGACAG GCCGGAGTGT GGCCAGCGTG 
ACGTGGGCTG ATGGTACCAC CAATGTGTAC CGTGTGGGCC ACAAGGGCAA GGTGGACCTC 
AAGTGTGTGG GCGAGGCAGC GGGCGGCTTC TACTACAAGG ACCACCTCCC AAGGCTCGGC 
AAGCCGGCGG AGCTGCAGCG CAGGGTGAGT GCTGACAGCC AGCCCTTCCA GCACGGGGAC 
AAGGTCAAGT GTCTGCTGGA CACTGATGTC CTGCGGGAGA TGCAGGAAGG CCACGGCGGC 
TGGAACCCCA GGATGGCGGA GTTTATCGGA CAGACGGGCA CCGTGCATCG TATCACGGAC 
CGCGGGGACG TGCGCGTGCA GTTCAACCAC GAGACGCGCT GGACCTTCCA CCCCGGGGCG 
CTCACCAAGC ACCACTCCTT CTGGGTGGGC GACGTGGTCC GGGTCATCGG CGACCTTGAC 
ACAGTGAAGC GGCTGCAGGC TGGGCATGGC GAGTGGACGG ACGACATGGC CCCTGCCCTG 
GGCCGCGTCG GGAAGGTGGT GAAAGTGTTT GGAGACGGGA ACCTGCGTGT AGCAGTCGCT 
GGTCAGCGGT GGACCTTCAG CCCCTCCTGC CTGGTGGCCT ACCGGCCCGA GGAGGATGCC 
AACCTGGACG TGGCCGAGCG CGCCCGGGAG AACAAAAGCT CACTGAGCGT GGCCCTGGAC 
AAGCTTCGGG CCCAGAAGAG TGACCCAGAG CACCCGGGAA GGCTGGTGGT GGAGGTGGCG 
CTGGGTAACG CAGCCCGGGC TCTGGACCTG CTGCGGAGGC GCCCAGAGCA GGTGGACACC 
AAGAACCAAG GCAGGACCGC TCTGCAAGTG GCTGCCTACC TGGGCCAGGT GGAGTTGATA 
CGGCTGCTGC TACAAGCCAG GGCGGGCGTG GACCTGCCGG ACGACGAGGG CAACACGGCA 
CTGCACTACG CGGCCCTGGG GAACCAGCCC GAGGCCACCA GGGTGCTCCT GAGTGCTGGG 
TGCCGGGCGG ACGCCATCAA CAGCACCCAG AGCACAGCAC TGCACGTGGC CGTGCAGAGG 
GGCTTCCTGG AGGTGGTGCG GGCCCTGTGT GAGCGCGGCT GTGACGTCAA CCTGCCCGAC 
GCCCACTCGG ACACGCCCCT GCACTCCGCC ATCTCGGCGG GCACTGGAGC CAGCGGCATT 
GTCGAGGTCC TCACGGAGGT GCCAAACATC GATGTTACCG CCACCAACAG CCAGGGTTTC 
ACCCTGCTGC ACCATGCCTC CCTCAAGGGT CACGCGCTAG CTGTGAGAAA GATTCTGGCT 
CGGGCGCGGC AGCTGGTGGA CGCCAAGAAG GAGGACGGCT TCACGGCGCT GCATCTGGCT 
GCCCTCAACA ACCACCGCGA GGTGGCCCAG ATCCTCATCC GGGAGGGCCG CTGTGACGTG 
AACGTGCGCA ACCGGAAGCT GCAGTCCCCG CTGCATCTCG CCGTGCAACA GGCCCACGTG 
GGGCTGGTGC CGCTACTGGT GGACGCTGGG TGCAGTGTCA ACGCCGAGGA CGAGGAGGGG 
GACACAGCCC TGCACGTGGC GCTGCAGCGT CATCAGCTGC TGCCCCTGGT GGCTGATGGG 
GCCGGGGGGG ACCCAGGGCC CTTGCAGCTG CTGTCCAGGA CATGGGGCGC CGGCTGCTGG 
GGCTGCCAGG TGCCAGGAGA CGCCTCCCTC GGGCCTGCCC CGGCGCCCGC CCTCACCGGC 
GTCTGTCCTG CCGCCCAGCT ACAGGCCTCG GGCCTCCCCG GCAGCGCGGA GCTGACGGTG 
GGCGCGGCGG TCGCCTGCTT CCTGGCGCTG GAGGGCGCCG ACGTGAGCTA CACCAACCAC 
CGCGGTCGGA GCCCGCTGGA CCTGGCCGCC GAGGGTCGCG TGCTCAAGGC CCTTCAGGGC 
TGCGCCCAGC GCTTCCGGGA GCGGCAGGCG GGCGGGGGCG CGGCCCCGGG CCCCAGGCAA 
ACGCTCGGGA CCCCCAACAC CGTGACGAAC CTGCACGTGG GCGCCGCGCC GGGGCCCGAG 
GCCGCTGAGT GCCTGGTGTG CTCCGAGCTG GCGCTGCTGG TGCTGTTCTC GCCGTGCCAG 
CACCGCACCG TGTGTGAGGG TGAGTGGGGG GCCCCGGGGT GGGGAGGCCC GGCTAGTAGG 
GCCGCAGCCA ACCGCGCTCT CCTCTTCGCA GAGTGCGCGC GCAGGATGAA GAAGTGCATC 
AGGTGCCAGG TGGTCGTCAG CAAGAAACTG CGCCCAGACG GCTCTGAGGT GGCGAGCGCC 
GCCCCCGCCC CCGGCCCGCC GCGCCAGCTG GTGGAGGAGC TGCAGAGCCG CTACCGGCAG 
ATGGAGGAAC GCATCACCTG CCCCATCTGC ATCGACAGCC ACATCCGCCT CGTGTTCCAG 
TGCGGCCACG GCGCATGCGC CCCCTGCGGC TCCGCGCTCA GCGCCTGCCC CATCTGCCGC 
CAGCCCATCC GCGACCGCAT CCAGATCTTC GTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MIB2 variants altering NOTCH signalling result in left ventricle hypertrabeculation/non-compaction and are associated with M?n?trier-like gastropathy.
Human molecular genetics26(1)33-43(2017 01)
Piccolo P,Attanasio S,Secco I,Sangermano R,Strisciuglio C,Limongelli G,Miele E,Mutarelli M,Banfi S,Nigro V,Pons T,Valencia A,Zentilin L,Campione S,Nardone G,Lynnes TC,Celestino-Soper PB,Spoonamore KG,D'Armiento FP,Giacca M,Staiano A,Vatta M,Collesi C,Brunetti-Pierri N

The cylindromatosis gene product, CYLD, interacts with MIB2 to regulate notch signalling.
Oncotarget5(23)12126-40(2014 Dec)
Rajan N,Elliott RJ,Smith A,Sinclair N,Swift S,Lord CJ,Ashworth A

Lysine 63-linked TANK-binding kinase 1 ubiquitination by mindbomb E3 ubiquitin protein ligase 2 is mediated by the mitochondrial antiviral signaling protein.
Journal of virology88(21)12765-76(2014 Nov)
Ye JS,Kim N,Lee KJ,Nam YR,Lee U,Joo CH

The E3 ubiquitin ligase mind bomb-2 (MIB2) protein controls B-cell CLL/lymphoma 10 (BCL10)-dependent NF-?B activation.
The Journal of biological chemistry286(43)37147-57(2011 Oct)
Stempin CC,Chi L,Giraldo-Vela JP,High AA,H?cker H,Redecke V

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MIB2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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