mib2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following mib2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mib2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa21593 NM_001123444.1
Latest version!
Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa31374 XM_012966027.3
Latest version!
Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa31374 XM_012966025.3
Latest version!
Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OXa31375 XM_012966028.3
Latest version!
Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OXa31374 XM_012966027.2 Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa31374 XM_012966025.2 Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OXa31375 XM_012966028.2 Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa21593
    Clone ID Related Accession (Same CDS sequence) NM_001123444.1
    Accession Version NM_001123444.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-21
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase MIB2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC161083.1. ##Evidence-Data-START## Transcript exon combination :: BC161083.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
    TGGAAATGGT CAAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA
    CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC
    AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT
    CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT
    GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG
    AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA
    GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT
    GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA
    GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT
    GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT
    CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT
    GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA
    GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG
    GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA
    GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG
    GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG
    GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG
    CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT
    GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT
    GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA
    GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA
    GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT
    ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG
    GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT
    GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA
    GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC
    AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG
    GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT
    GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT
    CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA
    GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA
    CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT
    CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC
    TTGGTGCATC TCTTAGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT
    ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA
    GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG
    TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT
    GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT
    GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT
    TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT
    GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC
    CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA
    TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC
    AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA
    ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG
    TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG
    CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001116916.1
    CDS200..3055
    Translation

    Target ORF information:

    RefSeq Version NM_001123444.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001123444.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
    TGGAAATGGT CAAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA
    CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC
    AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT
    CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT
    GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG
    AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA
    GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT
    GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA
    GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT
    GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT
    CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT
    GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA
    GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG
    GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA
    GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG
    GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG
    GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG
    CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT
    GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT
    GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA
    GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA
    GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT
    ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG
    GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT
    GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA
    GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC
    AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG
    GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT
    GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT
    CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA
    GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA
    CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT
    CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC
    TTGGTGCATC TCTTAGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT
    ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA
    GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG
    TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT
    GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT
    GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT
    TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT
    GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC
    CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA
    TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC
    AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA
    ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG
    TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG
    CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31374
    Clone ID Related Accession (Same CDS sequence) XM_012966027.3 , XM_012966025.2 , XM_012966027.2 , XM_012966025.3
    Accession Version XM_012966025.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966025.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
    TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA
    CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC
    AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT
    CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT
    GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG
    AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA
    GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT
    GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA
    GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT
    GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT
    CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT
    GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA
    GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG
    GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA
    GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG
    GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG
    GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG
    CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT
    GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT
    GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA
    GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA
    GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT
    ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG
    GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT
    GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA
    GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC
    AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG
    GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT
    GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT
    CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA
    GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA
    CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT
    CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC
    TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT
    ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA
    GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG
    TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT
    GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT
    GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT
    TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT
    GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC
    CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA
    TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC
    AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA
    ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG
    TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG
    CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821479.1
    CDS154..3009
    Translation

    Target ORF information:

    RefSeq Version XM_012966025.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966025.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
    TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA
    CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC
    AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT
    CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT
    GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG
    AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA
    GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT
    GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA
    GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT
    GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT
    CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT
    GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA
    GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG
    GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA
    GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG
    GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG
    GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG
    CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT
    GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT
    GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA
    GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA
    GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT
    ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG
    GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT
    GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA
    GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC
    AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG
    GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT
    GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT
    CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA
    GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA
    CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT
    CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC
    TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT
    ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA
    GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG
    TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT
    GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT
    GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT
    TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT
    GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC
    CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA
    TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC
    AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA
    ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG
    TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG
    CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31374
    Clone ID Related Accession (Same CDS sequence) XM_012966027.3 , XM_012966025.2 , XM_012966027.2 , XM_012966025.3
    Accession Version XM_012966027.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966027.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
    TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA
    CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC
    AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT
    CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT
    GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG
    AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA
    GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT
    GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA
    GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT
    GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT
    CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT
    GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA
    GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG
    GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA
    GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG
    GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG
    GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG
    CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT
    GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT
    GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA
    GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA
    GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT
    ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG
    GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT
    GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA
    GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC
    AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG
    GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT
    GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT
    CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA
    GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA
    CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT
    CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC
    TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT
    ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA
    GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG
    TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT
    GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT
    GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT
    TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT
    GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC
    CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA
    TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC
    AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA
    ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG
    TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG
    CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821481.1
    CDS671..3526
    Translation

    Target ORF information:

    RefSeq Version XM_012966027.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966027.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
    TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA
    CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC
    AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT
    CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT
    GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG
    AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA
    GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT
    GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA
    GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT
    GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT
    CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT
    GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA
    GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG
    GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA
    GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG
    GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG
    GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG
    CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT
    GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT
    GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA
    GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA
    GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT
    ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG
    GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT
    GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA
    GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC
    AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG
    GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT
    GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT
    CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA
    GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA
    CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT
    CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC
    TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT
    ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA
    GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG
    TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT
    GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT
    GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT
    TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT
    GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC
    CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA
    TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC
    AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA
    ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG
    TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG
    CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31375
    Clone ID Related Accession (Same CDS sequence) XM_012966028.3 , XM_012966028.2
    Accession Version XM_012966028.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966028.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
    TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA
    CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC
    AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT
    CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT
    GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG
    AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA
    GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT
    GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA
    GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT
    GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT
    CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT
    GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA
    GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG
    GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA
    GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG
    GCTCTAACAA AGGCGCTGGG TCAAATTGGC AAGGTGATCA AAGTTTTTGG GGATGGAGAC
    ATGCGAGTTT CTGTTGGAGG CCAGTCCTGG ACATTCAACC CTGCGTGCCT CACGTCATAC
    CAACGGGAAG AGGATGCTAA CCTCATGACC ACTGAAAATG CCAAGGAGTC TAAAAACACC
    CTGGTTTCCA TATTAGAAAA GTTGCTTTCT CAGAAGACTG ATTGTGAAAG TCCTACTTCC
    TTGGTTATTG AAGCAGCCCA GGGCAATACA GCCAAAGTCA GGGAGATGCT CCAGAAATAC
    CCCGACAAGG TGGATATTAA GAATCAGGGC AGGACGGCTT TGCAGGTTGC CTCCCATTTA
    GGCTATATGG AGGTGGTGAA GGTTTTGCTT CAAGCCAATG CAAACATTGA CCTCAGGGAT
    GACGAGGGGG ACACTGCTTT GCATTATGCT GCTTATGGAA ACCAGGCTGG TGTGGTTAGA
    GTTTTGCTAT CTAAAGGAGC CAATGCAGAA CTTCTAAACA ATGCCAAGTG TACAGCGCTC
    TACATTGCAG TTAACAAAGG GTTCACAGAA GTAGTCCAAG TCCTCTGCAA CCCAAATTGT
    GCCATCAACA TGCAGGATTC CTTTGGGGAC ACACCTCTGC ATTATGCAAT CACCGCAGAC
    TTTAGAAGCA TCATTGAGAT CCTCACTGAA GTACCCAATA TAGATTTCAC TGTGCAGAAC
    AATCAAGGGT TTAATCTACT GCATCACTCA GCTCTCAAGG GAAATGTCCT GGCTGTAAGC
    AAGATCCTGG AGAGAGCAAG ACAGCTTGTG GACTCTAAAA AAGAAGATGG TTTCACTGCC
    CTTCATCTTG CAACACTGAA CAACCACCAG GAGGTGGTAG AAATATTGAT CAAAGAGGGC
    CGCTGTGATG TCAATCTCAG GAATAACAGA AACCAGACCC CTCTGCATTT GGCTGTCGCC
    CAGGGCCACA TCAGCTTGGT GCATCTCTTG GTGACTGAGG GTGCCGATGT GAATGCAGAG
    GATGAGGATG GAGATACCCC AATGCACATA GTATTTGTGC GACAGCATCT CAAATCCATT
    GACTCTCAGC AAGAAGGCAA TGGGTCATCA CTACTCACAA AGTTGGAGGC CTCTGGGTTG
    CCGGGAAATA TAGAGTTAAA TGTTGGTTCA GCCATGGCTT GCTTTTTAGC TCTGTCTGGA
    GCTGACATTA ACTATGCCAA TCACCGTGGG AAGTCTCCCC TGGACCTGAT CGCAGATGGA
    AGGATAACGC ACCTTGTGAA AAACTTTGCA CAGAAATTCA GAGAACAAGT GGAGTTAGAC
    TCACCAACCG TCAGTTGCAG CCTCCGCAGA GTCCACACTA CACCTAATAC TATGACTAAC
    CTTAGCCCCT GTAGTGCCGC CAGTCCTGCA GAGTGTCTAG TATGCTCTGA GCTGGCCGTA
    CTAGTCAGTT TCTTCCCCTG CCAACACAGC ATAGTATGTG AAGAATGTTC CAGACGAATG
    AAGAAGTGCA TCAAATGTCA AGTGTGCATA GCAAGAAAGC TTAGGCAAGA CAGTACTGAA
    GTTGAATGCA GTCCCAGTTT AGAAGTGTCA GACCAGAAGA AAGTAATGGA GGAGCTCCAG
    AATCGATACC GCCAAATGGA GGAAAGGATC ACCTGTCCTA TTTGTATCGA CAATCACATC
    AAGCTGGTGT TCCAGTGTGG ACATGGGTCT TGTACTGAAT GCAGCACTTC CCTGACTGCC
    TGTCCCATAT GCAGGCAGCT TATCAGAGAG AGGATACAGA TATTTGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821482.1
    CDS483..3233
    Translation

    Target ORF information:

    RefSeq Version XM_012966028.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966028.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
    TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA
    CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC
    AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT
    CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT
    GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG
    AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA
    GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT
    GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA
    GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT
    GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT
    CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT
    GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA
    GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG
    GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA
    GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG
    GCTCTAACAA AGGCGCTGGG TCAAATTGGC AAGGTGATCA AAGTTTTTGG GGATGGAGAC
    ATGCGAGTTT CTGTTGGAGG CCAGTCCTGG ACATTCAACC CTGCGTGCCT CACGTCATAC
    CAACGGGAAG AGGATGCTAA CCTCATGACC ACTGAAAATG CCAAGGAGTC TAAAAACACC
    CTGGTTTCCA TATTAGAAAA GTTGCTTTCT CAGAAGACTG ATTGTGAAAG TCCTACTTCC
    TTGGTTATTG AAGCAGCCCA GGGCAATACA GCCAAAGTCA GGGAGATGCT CCAGAAATAC
    CCCGACAAGG TGGATATTAA GAATCAGGGC AGGACGGCTT TGCAGGTTGC CTCCCATTTA
    GGCTATATGG AGGTGGTGAA GGTTTTGCTT CAAGCCAATG CAAACATTGA CCTCAGGGAT
    GACGAGGGGG ACACTGCTTT GCATTATGCT GCTTATGGAA ACCAGGCTGG TGTGGTTAGA
    GTTTTGCTAT CTAAAGGAGC CAATGCAGAA CTTCTAAACA ATGCCAAGTG TACAGCGCTC
    TACATTGCAG TTAACAAAGG GTTCACAGAA GTAGTCCAAG TCCTCTGCAA CCCAAATTGT
    GCCATCAACA TGCAGGATTC CTTTGGGGAC ACACCTCTGC ATTATGCAAT CACCGCAGAC
    TTTAGAAGCA TCATTGAGAT CCTCACTGAA GTACCCAATA TAGATTTCAC TGTGCAGAAC
    AATCAAGGGT TTAATCTACT GCATCACTCA GCTCTCAAGG GAAATGTCCT GGCTGTAAGC
    AAGATCCTGG AGAGAGCAAG ACAGCTTGTG GACTCTAAAA AAGAAGATGG TTTCACTGCC
    CTTCATCTTG CAACACTGAA CAACCACCAG GAGGTGGTAG AAATATTGAT CAAAGAGGGC
    CGCTGTGATG TCAATCTCAG GAATAACAGA AACCAGACCC CTCTGCATTT GGCTGTCGCC
    CAGGGCCACA TCAGCTTGGT GCATCTCTTG GTGACTGAGG GTGCCGATGT GAATGCAGAG
    GATGAGGATG GAGATACCCC AATGCACATA GTATTTGTGC GACAGCATCT CAAATCCATT
    GACTCTCAGC AAGAAGGCAA TGGGTCATCA CTACTCACAA AGTTGGAGGC CTCTGGGTTG
    CCGGGAAATA TAGAGTTAAA TGTTGGTTCA GCCATGGCTT GCTTTTTAGC TCTGTCTGGA
    GCTGACATTA ACTATGCCAA TCACCGTGGG AAGTCTCCCC TGGACCTGAT CGCAGATGGA
    AGGATAACGC ACCTTGTGAA AAACTTTGCA CAGAAATTCA GAGAACAAGT GGAGTTAGAC
    TCACCAACCG TCAGTTGCAG CCTCCGCAGA GTCCACACTA CACCTAATAC TATGACTAAC
    CTTAGCCCCT GTAGTGCCGC CAGTCCTGCA GAGTGTCTAG TATGCTCTGA GCTGGCCGTA
    CTAGTCAGTT TCTTCCCCTG CCAACACAGC ATAGTATGTG AAGAATGTTC CAGACGAATG
    AAGAAGTGCA TCAAATGTCA AGTGTGCATA GCAAGAAAGC TTAGGCAAGA CAGTACTGAA
    GTTGAATGCA GTCCCAGTTT AGAAGTGTCA GACCAGAAGA AAGTAATGGA GGAGCTCCAG
    AATCGATACC GCCAAATGGA GGAAAGGATC ACCTGTCCTA TTTGTATCGA CAATCACATC
    AAGCTGGTGT TCCAGTGTGG ACATGGGTCT TGTACTGAAT GCAGCACTTC CCTGACTGCC
    TGTCCCATAT GCAGGCAGCT TATCAGAGAG AGGATACAGA TATTTGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31374
    Clone ID Related Accession (Same CDS sequence) XM_012966027.3 , XM_012966025.2 , XM_012966027.2 , XM_012966025.3
    Accession Version XM_012966027.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966027.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
    TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA
    CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC
    AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT
    CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT
    GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG
    AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA
    GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT
    GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA
    GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT
    GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT
    CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT
    GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA
    GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG
    GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA
    GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG
    GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG
    GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG
    CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT
    GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT
    GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA
    GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA
    GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT
    ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG
    GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT
    GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA
    GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC
    AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG
    GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT
    GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT
    CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA
    GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA
    CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT
    CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC
    TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT
    ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA
    GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG
    TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT
    GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT
    GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT
    TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT
    GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC
    CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA
    TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC
    AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA
    ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG
    TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG
    CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821481.1
    CDS254..3109
    Translation

    Target ORF information:

    RefSeq Version XM_012966027.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966027.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
    TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA
    CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC
    AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT
    CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT
    GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG
    AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA
    GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT
    GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA
    GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT
    GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT
    CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT
    GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA
    GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG
    GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA
    GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG
    GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG
    GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG
    CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT
    GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT
    GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA
    GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA
    GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT
    ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG
    GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT
    GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA
    GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC
    AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG
    GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT
    GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT
    CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA
    GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA
    CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT
    CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC
    TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT
    ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA
    GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG
    TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT
    GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT
    GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT
    TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT
    GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC
    CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA
    TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC
    AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA
    ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG
    TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG
    CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31374
    Clone ID Related Accession (Same CDS sequence) XM_012966027.3 , XM_012966025.2 , XM_012966027.2 , XM_012966025.3
    Accession Version XM_012966025.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2856bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966025.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
    TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA
    CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC
    AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT
    CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT
    GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG
    AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA
    GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT
    GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA
    GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT
    GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT
    CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT
    GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA
    GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG
    GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA
    GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG
    GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG
    GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG
    CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT
    GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT
    GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA
    GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA
    GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT
    ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG
    GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT
    GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA
    GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC
    AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG
    GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT
    GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT
    CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA
    GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA
    CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT
    CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC
    TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT
    ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA
    GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG
    TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT
    GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT
    GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT
    TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT
    GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC
    CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA
    TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC
    AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA
    ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG
    TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG
    CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821479.1
    CDS154..3009
    Translation

    Target ORF information:

    RefSeq Version XM_012966025.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966025.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
    TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA
    CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC
    AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT
    CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT
    GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG
    AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA
    GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT
    GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA
    GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT
    GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT
    CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT
    GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA
    GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG
    GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA
    GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG
    GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG
    GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG
    CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT
    GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT
    GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA
    GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA
    GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT
    ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG
    GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT
    GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA
    GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC
    AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG
    GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT
    GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT
    CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA
    GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA
    CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT
    CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC
    TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT
    ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA
    GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG
    TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT
    GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT
    GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT
    TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT
    GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC
    CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA
    TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC
    AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA
    ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG
    TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG
    CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa31375
    Clone ID Related Accession (Same CDS sequence) XM_012966028.3 , XM_012966028.2
    Accession Version XM_012966028.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2751bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product E3 ubiquitin-protein ligase MIB2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966028.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
    TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA
    CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC
    AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT
    CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT
    GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG
    AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA
    GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT
    GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA
    GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT
    GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT
    CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT
    GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA
    GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG
    GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA
    GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG
    GCTCTAACAA AGGCGCTGGG TCAAATTGGC AAGGTGATCA AAGTTTTTGG GGATGGAGAC
    ATGCGAGTTT CTGTTGGAGG CCAGTCCTGG ACATTCAACC CTGCGTGCCT CACGTCATAC
    CAACGGGAAG AGGATGCTAA CCTCATGACC ACTGAAAATG CCAAGGAGTC TAAAAACACC
    CTGGTTTCCA TATTAGAAAA GTTGCTTTCT CAGAAGACTG ATTGTGAAAG TCCTACTTCC
    TTGGTTATTG AAGCAGCCCA GGGCAATACA GCCAAAGTCA GGGAGATGCT CCAGAAATAC
    CCCGACAAGG TGGATATTAA GAATCAGGGC AGGACGGCTT TGCAGGTTGC CTCCCATTTA
    GGCTATATGG AGGTGGTGAA GGTTTTGCTT CAAGCCAATG CAAACATTGA CCTCAGGGAT
    GACGAGGGGG ACACTGCTTT GCATTATGCT GCTTATGGAA ACCAGGCTGG TGTGGTTAGA
    GTTTTGCTAT CTAAAGGAGC CAATGCAGAA CTTCTAAACA ATGCCAAGTG TACAGCGCTC
    TACATTGCAG TTAACAAAGG GTTCACAGAA GTAGTCCAAG TCCTCTGCAA CCCAAATTGT
    GCCATCAACA TGCAGGATTC CTTTGGGGAC ACACCTCTGC ATTATGCAAT CACCGCAGAC
    TTTAGAAGCA TCATTGAGAT CCTCACTGAA GTACCCAATA TAGATTTCAC TGTGCAGAAC
    AATCAAGGGT TTAATCTACT GCATCACTCA GCTCTCAAGG GAAATGTCCT GGCTGTAAGC
    AAGATCCTGG AGAGAGCAAG ACAGCTTGTG GACTCTAAAA AAGAAGATGG TTTCACTGCC
    CTTCATCTTG CAACACTGAA CAACCACCAG GAGGTGGTAG AAATATTGAT CAAAGAGGGC
    CGCTGTGATG TCAATCTCAG GAATAACAGA AACCAGACCC CTCTGCATTT GGCTGTCGCC
    CAGGGCCACA TCAGCTTGGT GCATCTCTTG GTGACTGAGG GTGCCGATGT GAATGCAGAG
    GATGAGGATG GAGATACCCC AATGCACATA GTATTTGTGC GACAGCATCT CAAATCCATT
    GACTCTCAGC AAGAAGGCAA TGGGTCATCA CTACTCACAA AGTTGGAGGC CTCTGGGTTG
    CCGGGAAATA TAGAGTTAAA TGTTGGTTCA GCCATGGCTT GCTTTTTAGC TCTGTCTGGA
    GCTGACATTA ACTATGCCAA TCACCGTGGG AAGTCTCCCC TGGACCTGAT CGCAGATGGA
    AGGATAACGC ACCTTGTGAA AAACTTTGCA CAGAAATTCA GAGAACAAGT GGAGTTAGAC
    TCACCAACCG TCAGTTGCAG CCTCCGCAGA GTCCACACTA CACCTAATAC TATGACTAAC
    CTTAGCCCCT GTAGTGCCGC CAGTCCTGCA GAGTGTCTAG TATGCTCTGA GCTGGCCGTA
    CTAGTCAGTT TCTTCCCCTG CCAACACAGC ATAGTATGTG AAGAATGTTC CAGACGAATG
    AAGAAGTGCA TCAAATGTCA AGTGTGCATA GCAAGAAAGC TTAGGCAAGA CAGTACTGAA
    GTTGAATGCA GTCCCAGTTT AGAAGTGTCA GACCAGAAGA AAGTAATGGA GGAGCTCCAG
    AATCGATACC GCCAAATGGA GGAAAGGATC ACCTGTCCTA TTTGTATCGA CAATCACATC
    AAGCTGGTGT TCCAGTGTGG ACATGGGTCT TGTACTGAAT GCAGCACTTC CCTGACTGCC
    TGTCCCATAT GCAGGCAGCT TATCAGAGAG AGGATACAGA TATTTGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012821482.1
    CDS471..3221
    Translation

    Target ORF information:

    RefSeq Version XM_012966028.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012966028.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
    TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA
    CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC
    AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT
    CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT
    GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG
    AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA
    GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT
    GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA
    GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT
    GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT
    CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT
    GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA
    GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG
    GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA
    GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG
    GCTCTAACAA AGGCGCTGGG TCAAATTGGC AAGGTGATCA AAGTTTTTGG GGATGGAGAC
    ATGCGAGTTT CTGTTGGAGG CCAGTCCTGG ACATTCAACC CTGCGTGCCT CACGTCATAC
    CAACGGGAAG AGGATGCTAA CCTCATGACC ACTGAAAATG CCAAGGAGTC TAAAAACACC
    CTGGTTTCCA TATTAGAAAA GTTGCTTTCT CAGAAGACTG ATTGTGAAAG TCCTACTTCC
    TTGGTTATTG AAGCAGCCCA GGGCAATACA GCCAAAGTCA GGGAGATGCT CCAGAAATAC
    CCCGACAAGG TGGATATTAA GAATCAGGGC AGGACGGCTT TGCAGGTTGC CTCCCATTTA
    GGCTATATGG AGGTGGTGAA GGTTTTGCTT CAAGCCAATG CAAACATTGA CCTCAGGGAT
    GACGAGGGGG ACACTGCTTT GCATTATGCT GCTTATGGAA ACCAGGCTGG TGTGGTTAGA
    GTTTTGCTAT CTAAAGGAGC CAATGCAGAA CTTCTAAACA ATGCCAAGTG TACAGCGCTC
    TACATTGCAG TTAACAAAGG GTTCACAGAA GTAGTCCAAG TCCTCTGCAA CCCAAATTGT
    GCCATCAACA TGCAGGATTC CTTTGGGGAC ACACCTCTGC ATTATGCAAT CACCGCAGAC
    TTTAGAAGCA TCATTGAGAT CCTCACTGAA GTACCCAATA TAGATTTCAC TGTGCAGAAC
    AATCAAGGGT TTAATCTACT GCATCACTCA GCTCTCAAGG GAAATGTCCT GGCTGTAAGC
    AAGATCCTGG AGAGAGCAAG ACAGCTTGTG GACTCTAAAA AAGAAGATGG TTTCACTGCC
    CTTCATCTTG CAACACTGAA CAACCACCAG GAGGTGGTAG AAATATTGAT CAAAGAGGGC
    CGCTGTGATG TCAATCTCAG GAATAACAGA AACCAGACCC CTCTGCATTT GGCTGTCGCC
    CAGGGCCACA TCAGCTTGGT GCATCTCTTG GTGACTGAGG GTGCCGATGT GAATGCAGAG
    GATGAGGATG GAGATACCCC AATGCACATA GTATTTGTGC GACAGCATCT CAAATCCATT
    GACTCTCAGC AAGAAGGCAA TGGGTCATCA CTACTCACAA AGTTGGAGGC CTCTGGGTTG
    CCGGGAAATA TAGAGTTAAA TGTTGGTTCA GCCATGGCTT GCTTTTTAGC TCTGTCTGGA
    GCTGACATTA ACTATGCCAA TCACCGTGGG AAGTCTCCCC TGGACCTGAT CGCAGATGGA
    AGGATAACGC ACCTTGTGAA AAACTTTGCA CAGAAATTCA GAGAACAAGT GGAGTTAGAC
    TCACCAACCG TCAGTTGCAG CCTCCGCAGA GTCCACACTA CACCTAATAC TATGACTAAC
    CTTAGCCCCT GTAGTGCCGC CAGTCCTGCA GAGTGTCTAG TATGCTCTGA GCTGGCCGTA
    CTAGTCAGTT TCTTCCCCTG CCAACACAGC ATAGTATGTG AAGAATGTTC CAGACGAATG
    AAGAAGTGCA TCAAATGTCA AGTGTGCATA GCAAGAAAGC TTAGGCAAGA CAGTACTGAA
    GTTGAATGCA GTCCCAGTTT AGAAGTGTCA GACCAGAAGA AAGTAATGGA GGAGCTCCAG
    AATCGATACC GCCAAATGGA GGAAAGGATC ACCTGTCCTA TTTGTATCGA CAATCACATC
    AAGCTGGTGT TCCAGTGTGG ACATGGGTCT TGTACTGAAT GCAGCACTTC CCTGACTGCC
    TGTCCCATAT GCAGGCAGCT TATCAGAGAG AGGATACAGA TATTTGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.