mib2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	mib2
Entrez Gene ID	100144683
Full Name	mindbomb E3 ubiquitin protein ligase 2
Synonyms	zzank1,zzz5
General protein information	Preferred Names mindbomb E3 ubiquitin protein ligase 2 Names E3 ubiquitin-protein ligase MIB2 mindbomb homolog 2
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012966027.3	XP_012821481.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_012966025.2	XP_012821479.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_012966027.2	XP_012821481.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_012966025.3	XP_012821479.1	E3 ubiquitin-protein ligase MIB2 isoform X1
XM_012966028.3	XP_012821482.1	E3 ubiquitin-protein ligase MIB2 isoform X2
NM_001123444.1	NP_001116916.1	E3 ubiquitin-protein ligase MIB2
XM_012966028.2	XP_012821482.1	E3 ubiquitin-protein ligase MIB2 isoform X2

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mib2	NP_001005901.2
Xenopus tropicalis (tropical clawed frog)	mib2	NP_001116916.1
Homo sapiens (human)	MIB2	NP_543151.2
Bos taurus (cattle)	MIB2	XP_002694171.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008851	XP_319593.4
Gallus gallus (chicken)	MIB2	NP_001006301.1
Drosophila melanogaster (fruit fly)	mib2	NP_001260554.1
Pan troglodytes (chimpanzee)	MIB2	XP_001155032.3
Mus musculus (house mouse)	Mib2	NP_001243036.1
	MIB2	XP_001096926.2
Canis lupus familiaris (dog)	MIB2	XP_005620446.1
Danio rerio (zebrafish)	mib2	NP_001073146.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following mib2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mib2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa21593	NM_001123444.1 Latest version!	Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa31374	XM_012966027.3 Latest version!	Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa31374	XM_012966025.3 Latest version!	Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa31375	XM_012966028.3 Latest version!	Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OXa31374	XM_012966027.2	Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa31374	XM_012966025.2	Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa31375	XM_012966028.2	Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mib2 ( NM_001123444.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001116916.1 Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), mRNA.

Price & Availability↑

CloneID	OXa21593
Clone ID Related Accession (Same CDS sequence)	NM_001123444.1
Accession Version	NM_001123444.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase MIB2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC161083.1. ##Evidence-Data-START## Transcript exon combination :: BC161083.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
TGGAAATGGT CAAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA 
CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC 
AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT 
CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT 
GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG 
AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA 
GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT 
GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA 
GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT 
GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT 
CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT 
GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA 
GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG 
GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA 
GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG 
GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG 
GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG 
CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT 
GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT 
GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA 
GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA 
GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT 
ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG 
GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT 
GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA 
GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC 
AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG 
GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT 
GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT 
CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA 
GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA 
CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT 
CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC 
TTGGTGCATC TCTTAGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT 
ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA 
GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG 
TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT 
GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT 
GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT 
TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT 
GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC 
CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA 
TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC 
AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA 
ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG 
TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG 
CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001116916.1
CDS	200..3055
Translation	MDWDNSAAMQVGMRVVRGIDWKWSNQDNGEGSMGTVVEIGRQGSPTTPDKTVVVQWDHGTRTNYRTGFQGAYDLLLYDNAQTGVRHPNIICDCCKKHGIRGMRWKCKVCFDYDLCTQCYMNNKHDLSHIFERYETADSRPVILSTRQGLPRVVLKGIFQGAKVVRGPDWEWGNQDGGEGKVGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCITDAPGGHYYRDHLPKLGKPAELQRKESSERHPFQHGDKVKCLLDVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERGDVRVQYNSETRWTFHPGALTKQNSFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKLLSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNPNCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILERARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNIELNVGSAMACFLALSGADINYANHRGKSPLDLIADGRITHLVKNFAQKFREQVELDSPTVSCSLRRVHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLRQDSTEVECSPSLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQIFV

Target ORF information:

RefSeq Version	NM_001123444.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001123444.1

ORF Insert Sequence:

ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
TGGAAATGGT CAAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA 
CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC 
AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT 
CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT 
GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG 
AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA 
GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT 
GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA 
GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT 
GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT 
CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT 
GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA 
GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG 
GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA 
GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG 
GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG 
GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG 
CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT 
GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT 
GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA 
GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA 
GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT 
ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG 
GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT 
GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA 
GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC 
AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG 
GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT 
GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT 
CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA 
GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA 
CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT 
CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC 
TTGGTGCATC TCTTAGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT 
ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA 
GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG 
TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT 
GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT 
GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT 
TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT 
GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC 
CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA 
TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC 
AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA 
ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG 
TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG 
CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib2 ( XM_012966025.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821479.1 Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa31374
Clone ID Related Accession (Same CDS sequence)	XM_012966027.3 , XM_012966025.2 , XM_012966027.2 , XM_012966025.3
Accession Version	XM_012966025.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966025.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA 
CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC 
AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT 
CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT 
GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG 
AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA 
GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT 
GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA 
GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT 
GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT 
CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT 
GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA 
GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG 
GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA 
GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG 
GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG 
GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG 
CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT 
GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT 
GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA 
GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA 
GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT 
ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG 
GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT 
GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA 
GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC 
AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG 
GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT 
GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT 
CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA 
GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA 
CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT 
CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC 
TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT 
ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA 
GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG 
TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT 
GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT 
GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT 
TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT 
GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC 
CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA 
TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC 
AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA 
ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG 
TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG 
CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821479.1
CDS	154..3009
Translation	MDWDNSAAMQVGMRVVRGIDWKWSNQDNGEGSMGTVVEIGRQGSPTTPDKTVVVQWDHGTRTNYRTGFQGAYDLLLYDNAQTGVRHPNIICDCCKKHGIRGMRWKCKVCFDYDLCTQCYMNNKHDLSHIFERYETADSRPVILSTRQGLPRVVLKGIFQGAKVVRGPDWEWGNQDGGEGKVGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCITDAPGGHYYRDHLPKLGKPAELQRKESSERHPFQHGDKVKCLLDVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERGDVRVQYNSETRWTFHPGALTKQNSFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKLLSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNPNCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILERARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNIELNVGSAMACFLALSGADINYANHRGKSPLDLIADGRITHLVKNFAQKFREQVELDSPTVSCSLRRVHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLRQDSTEVECSPSLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQIFV

Target ORF information:

RefSeq Version	XM_012966025.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966025.2

ORF Insert Sequence:

ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA 
CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC 
AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT 
CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT 
GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG 
AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA 
GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT 
GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA 
GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT 
GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT 
CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT 
GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA 
GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG 
GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA 
GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG 
GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG 
GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG 
CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT 
GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT 
GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA 
GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA 
GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT 
ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG 
GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT 
GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA 
GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC 
AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG 
GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT 
GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT 
CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA 
GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA 
CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT 
CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC 
TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT 
ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA 
GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG 
TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT 
GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT 
GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT 
TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT 
GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC 
CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA 
TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC 
AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA 
ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG 
TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG 
CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib2 ( XM_012966027.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821481.1 Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa31374
Clone ID Related Accession (Same CDS sequence)	XM_012966027.3 , XM_012966025.2 , XM_012966027.2 , XM_012966025.3
Accession Version	XM_012966027.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966027.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA 
CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC 
AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT 
CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT 
GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG 
AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA 
GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT 
GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA 
GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT 
GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT 
CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT 
GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA 
GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG 
GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA 
GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG 
GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG 
GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG 
CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT 
GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT 
GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA 
GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA 
GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT 
ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG 
GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT 
GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA 
GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC 
AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG 
GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT 
GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT 
CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA 
GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA 
CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT 
CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC 
TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT 
ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA 
GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG 
TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT 
GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT 
GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT 
TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT 
GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC 
CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA 
TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC 
AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA 
ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG 
TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG 
CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821481.1
CDS	671..3526
Translation	MDWDNSAAMQVGMRVVRGIDWKWSNQDNGEGSMGTVVEIGRQGSPTTPDKTVVVQWDHGTRTNYRTGFQGAYDLLLYDNAQTGVRHPNIICDCCKKHGIRGMRWKCKVCFDYDLCTQCYMNNKHDLSHIFERYETADSRPVILSTRQGLPRVVLKGIFQGAKVVRGPDWEWGNQDGGEGKVGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCITDAPGGHYYRDHLPKLGKPAELQRKESSERHPFQHGDKVKCLLDVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERGDVRVQYNSETRWTFHPGALTKQNSFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKLLSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNPNCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILERARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNIELNVGSAMACFLALSGADINYANHRGKSPLDLIADGRITHLVKNFAQKFREQVELDSPTVSCSLRRVHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLRQDSTEVECSPSLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQIFV

Target ORF information:

RefSeq Version	XM_012966027.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966027.2

ORF Insert Sequence:

ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA 
CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC 
AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT 
CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT 
GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG 
AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA 
GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT 
GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA 
GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT 
GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT 
CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT 
GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA 
GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG 
GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA 
GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG 
GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG 
GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG 
CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT 
GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT 
GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA 
GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA 
GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT 
ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG 
GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT 
GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA 
GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC 
AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG 
GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT 
GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT 
CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA 
GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA 
CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT 
CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC 
TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT 
ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA 
GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG 
TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT 
GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT 
GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT 
TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT 
GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC 
CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA 
TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC 
AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA 
ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG 
TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG 
CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib2 ( XM_012966028.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821482.1 Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa31375
Clone ID Related Accession (Same CDS sequence)	XM_012966028.3 , XM_012966028.2
Accession Version	XM_012966028.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012966028.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA 
CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC 
AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT 
CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT 
GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG 
AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA 
GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT 
GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA 
GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT 
GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT 
CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT 
GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA 
GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG 
GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA 
GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG 
GCTCTAACAA AGGCGCTGGG TCAAATTGGC AAGGTGATCA AAGTTTTTGG GGATGGAGAC 
ATGCGAGTTT CTGTTGGAGG CCAGTCCTGG ACATTCAACC CTGCGTGCCT CACGTCATAC 
CAACGGGAAG AGGATGCTAA CCTCATGACC ACTGAAAATG CCAAGGAGTC TAAAAACACC 
CTGGTTTCCA TATTAGAAAA GTTGCTTTCT CAGAAGACTG ATTGTGAAAG TCCTACTTCC 
TTGGTTATTG AAGCAGCCCA GGGCAATACA GCCAAAGTCA GGGAGATGCT CCAGAAATAC 
CCCGACAAGG TGGATATTAA GAATCAGGGC AGGACGGCTT TGCAGGTTGC CTCCCATTTA 
GGCTATATGG AGGTGGTGAA GGTTTTGCTT CAAGCCAATG CAAACATTGA CCTCAGGGAT 
GACGAGGGGG ACACTGCTTT GCATTATGCT GCTTATGGAA ACCAGGCTGG TGTGGTTAGA 
GTTTTGCTAT CTAAAGGAGC CAATGCAGAA CTTCTAAACA ATGCCAAGTG TACAGCGCTC 
TACATTGCAG TTAACAAAGG GTTCACAGAA GTAGTCCAAG TCCTCTGCAA CCCAAATTGT 
GCCATCAACA TGCAGGATTC CTTTGGGGAC ACACCTCTGC ATTATGCAAT CACCGCAGAC 
TTTAGAAGCA TCATTGAGAT CCTCACTGAA GTACCCAATA TAGATTTCAC TGTGCAGAAC 
AATCAAGGGT TTAATCTACT GCATCACTCA GCTCTCAAGG GAAATGTCCT GGCTGTAAGC 
AAGATCCTGG AGAGAGCAAG ACAGCTTGTG GACTCTAAAA AAGAAGATGG TTTCACTGCC 
CTTCATCTTG CAACACTGAA CAACCACCAG GAGGTGGTAG AAATATTGAT CAAAGAGGGC 
CGCTGTGATG TCAATCTCAG GAATAACAGA AACCAGACCC CTCTGCATTT GGCTGTCGCC 
CAGGGCCACA TCAGCTTGGT GCATCTCTTG GTGACTGAGG GTGCCGATGT GAATGCAGAG 
GATGAGGATG GAGATACCCC AATGCACATA GTATTTGTGC GACAGCATCT CAAATCCATT 
GACTCTCAGC AAGAAGGCAA TGGGTCATCA CTACTCACAA AGTTGGAGGC CTCTGGGTTG 
CCGGGAAATA TAGAGTTAAA TGTTGGTTCA GCCATGGCTT GCTTTTTAGC TCTGTCTGGA 
GCTGACATTA ACTATGCCAA TCACCGTGGG AAGTCTCCCC TGGACCTGAT CGCAGATGGA 
AGGATAACGC ACCTTGTGAA AAACTTTGCA CAGAAATTCA GAGAACAAGT GGAGTTAGAC 
TCACCAACCG TCAGTTGCAG CCTCCGCAGA GTCCACACTA CACCTAATAC TATGACTAAC 
CTTAGCCCCT GTAGTGCCGC CAGTCCTGCA GAGTGTCTAG TATGCTCTGA GCTGGCCGTA 
CTAGTCAGTT TCTTCCCCTG CCAACACAGC ATAGTATGTG AAGAATGTTC CAGACGAATG 
AAGAAGTGCA TCAAATGTCA AGTGTGCATA GCAAGAAAGC TTAGGCAAGA CAGTACTGAA 
GTTGAATGCA GTCCCAGTTT AGAAGTGTCA GACCAGAAGA AAGTAATGGA GGAGCTCCAG 
AATCGATACC GCCAAATGGA GGAAAGGATC ACCTGTCCTA TTTGTATCGA CAATCACATC 
AAGCTGGTGT TCCAGTGTGG ACATGGGTCT TGTACTGAAT GCAGCACTTC CCTGACTGCC 
TGTCCCATAT GCAGGCAGCT TATCAGAGAG AGGATACAGA TATTTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821482.1
CDS	483..3233
Translation	MDWDNSAAMQVGMRVVRGIDWKWSNQDNGEGSMGTVVEIGRQGSPTTPDKTVVVQWDHGTRTNYRTGFQGAYDLLLYDNAQTGVRHPNIICDCCKKHGIRGMRWKCKVCFDYDLCTQCYMNNKHDLSHIFERYETADSRPVILSTRQGLPRVVLKGIFQGAKVVRGPDWEWGNQDGGEGKVGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCITDAPGGHYYRDHLPKLGKPAELQRKESSERHPFQHGDKVKCLLDVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERGDVRVQYNSETRWTFHPGALTKALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKLLSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNPNCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILERARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNIELNVGSAMACFLALSGADINYANHRGKSPLDLIADGRITHLVKNFAQKFREQVELDSPTVSCSLRRVHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLRQDSTEVECSPSLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQIFV

Target ORF information:

RefSeq Version	XM_012966028.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966028.2

ORF Insert Sequence:

ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA 
CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC 
AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT 
CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT 
GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG 
AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA 
GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT 
GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA 
GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT 
GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT 
CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT 
GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA 
GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG 
GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA 
GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG 
GCTCTAACAA AGGCGCTGGG TCAAATTGGC AAGGTGATCA AAGTTTTTGG GGATGGAGAC 
ATGCGAGTTT CTGTTGGAGG CCAGTCCTGG ACATTCAACC CTGCGTGCCT CACGTCATAC 
CAACGGGAAG AGGATGCTAA CCTCATGACC ACTGAAAATG CCAAGGAGTC TAAAAACACC 
CTGGTTTCCA TATTAGAAAA GTTGCTTTCT CAGAAGACTG ATTGTGAAAG TCCTACTTCC 
TTGGTTATTG AAGCAGCCCA GGGCAATACA GCCAAAGTCA GGGAGATGCT CCAGAAATAC 
CCCGACAAGG TGGATATTAA GAATCAGGGC AGGACGGCTT TGCAGGTTGC CTCCCATTTA 
GGCTATATGG AGGTGGTGAA GGTTTTGCTT CAAGCCAATG CAAACATTGA CCTCAGGGAT 
GACGAGGGGG ACACTGCTTT GCATTATGCT GCTTATGGAA ACCAGGCTGG TGTGGTTAGA 
GTTTTGCTAT CTAAAGGAGC CAATGCAGAA CTTCTAAACA ATGCCAAGTG TACAGCGCTC 
TACATTGCAG TTAACAAAGG GTTCACAGAA GTAGTCCAAG TCCTCTGCAA CCCAAATTGT 
GCCATCAACA TGCAGGATTC CTTTGGGGAC ACACCTCTGC ATTATGCAAT CACCGCAGAC 
TTTAGAAGCA TCATTGAGAT CCTCACTGAA GTACCCAATA TAGATTTCAC TGTGCAGAAC 
AATCAAGGGT TTAATCTACT GCATCACTCA GCTCTCAAGG GAAATGTCCT GGCTGTAAGC 
AAGATCCTGG AGAGAGCAAG ACAGCTTGTG GACTCTAAAA AAGAAGATGG TTTCACTGCC 
CTTCATCTTG CAACACTGAA CAACCACCAG GAGGTGGTAG AAATATTGAT CAAAGAGGGC 
CGCTGTGATG TCAATCTCAG GAATAACAGA AACCAGACCC CTCTGCATTT GGCTGTCGCC 
CAGGGCCACA TCAGCTTGGT GCATCTCTTG GTGACTGAGG GTGCCGATGT GAATGCAGAG 
GATGAGGATG GAGATACCCC AATGCACATA GTATTTGTGC GACAGCATCT CAAATCCATT 
GACTCTCAGC AAGAAGGCAA TGGGTCATCA CTACTCACAA AGTTGGAGGC CTCTGGGTTG 
CCGGGAAATA TAGAGTTAAA TGTTGGTTCA GCCATGGCTT GCTTTTTAGC TCTGTCTGGA 
GCTGACATTA ACTATGCCAA TCACCGTGGG AAGTCTCCCC TGGACCTGAT CGCAGATGGA 
AGGATAACGC ACCTTGTGAA AAACTTTGCA CAGAAATTCA GAGAACAAGT GGAGTTAGAC 
TCACCAACCG TCAGTTGCAG CCTCCGCAGA GTCCACACTA CACCTAATAC TATGACTAAC 
CTTAGCCCCT GTAGTGCCGC CAGTCCTGCA GAGTGTCTAG TATGCTCTGA GCTGGCCGTA 
CTAGTCAGTT TCTTCCCCTG CCAACACAGC ATAGTATGTG AAGAATGTTC CAGACGAATG 
AAGAAGTGCA TCAAATGTCA AGTGTGCATA GCAAGAAAGC TTAGGCAAGA CAGTACTGAA 
GTTGAATGCA GTCCCAGTTT AGAAGTGTCA GACCAGAAGA AAGTAATGGA GGAGCTCCAG 
AATCGATACC GCCAAATGGA GGAAAGGATC ACCTGTCCTA TTTGTATCGA CAATCACATC 
AAGCTGGTGT TCCAGTGTGG ACATGGGTCT TGTACTGAAT GCAGCACTTC CCTGACTGCC 
TGTCCCATAT GCAGGCAGCT TATCAGAGAG AGGATACAGA TATTTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib2 ( XM_012966027.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821481.1 Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa31374
Clone ID Related Accession (Same CDS sequence)	XM_012966027.3 , XM_012966025.2 , XM_012966027.2 , XM_012966025.3
Accession Version	XM_012966027.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966027.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA 
CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC 
AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT 
CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT 
GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG 
AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA 
GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT 
GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA 
GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT 
GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT 
CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT 
GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA 
GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG 
GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA 
GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG 
GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG 
GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG 
CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT 
GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT 
GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA 
GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA 
GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT 
ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG 
GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT 
GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA 
GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC 
AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG 
GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT 
GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT 
CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA 
GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA 
CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT 
CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC 
TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT 
ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA 
GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG 
TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT 
GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT 
GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT 
TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT 
GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC 
CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA 
TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC 
AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA 
ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG 
TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG 
CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821481.1
CDS	254..3109
Translation	MDWDNSAAMQVGMRVVRGIDWKWSNQDNGEGSMGTVVEIGRQGSPTTPDKTVVVQWDHGTRTNYRTGFQGAYDLLLYDNAQTGVRHPNIICDCCKKHGIRGMRWKCKVCFDYDLCTQCYMNNKHDLSHIFERYETADSRPVILSTRQGLPRVVLKGIFQGAKVVRGPDWEWGNQDGGEGKVGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCITDAPGGHYYRDHLPKLGKPAELQRKESSERHPFQHGDKVKCLLDVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERGDVRVQYNSETRWTFHPGALTKQNSFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKLLSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNPNCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILERARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNIELNVGSAMACFLALSGADINYANHRGKSPLDLIADGRITHLVKNFAQKFREQVELDSPTVSCSLRRVHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLRQDSTEVECSPSLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQIFV

Target ORF information:

RefSeq Version	XM_012966027.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966027.3

ORF Insert Sequence:

ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA 
CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC 
AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT 
CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT 
GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG 
AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA 
GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT 
GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA 
GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT 
GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT 
CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT 
GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA 
GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG 
GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA 
GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG 
GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG 
GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG 
CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT 
GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT 
GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA 
GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA 
GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT 
ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG 
GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT 
GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA 
GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC 
AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG 
GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT 
GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT 
CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA 
GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA 
CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT 
CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC 
TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT 
ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA 
GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG 
TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT 
GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT 
GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT 
TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT 
GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC 
CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA 
TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC 
AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA 
ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG 
TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG 
CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib2 ( XM_012966025.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821479.1 Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa31374
Clone ID Related Accession (Same CDS sequence)	XM_012966027.3 , XM_012966025.2 , XM_012966027.2 , XM_012966025.3
Accession Version	XM_012966025.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2856bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase MIB2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966025.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA 
CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC 
AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT 
CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT 
GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG 
AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA 
GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT 
GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA 
GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT 
GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT 
CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT 
GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA 
GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG 
GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA 
GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG 
GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG 
GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG 
CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT 
GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT 
GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA 
GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA 
GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT 
ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG 
GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT 
GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA 
GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC 
AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG 
GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT 
GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT 
CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA 
GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA 
CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT 
CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC 
TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT 
ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA 
GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG 
TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT 
GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT 
GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT 
TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT 
GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC 
CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA 
TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC 
AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA 
ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG 
TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG 
CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821479.1
CDS	154..3009
Translation	MDWDNSAAMQVGMRVVRGIDWKWSNQDNGEGSMGTVVEIGRQGSPTTPDKTVVVQWDHGTRTNYRTGFQGAYDLLLYDNAQTGVRHPNIICDCCKKHGIRGMRWKCKVCFDYDLCTQCYMNNKHDLSHIFERYETADSRPVILSTRQGLPRVVLKGIFQGAKVVRGPDWEWGNQDGGEGKVGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCITDAPGGHYYRDHLPKLGKPAELQRKESSERHPFQHGDKVKCLLDVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERGDVRVQYNSETRWTFHPGALTKQNSFWVGDVVRVMDNMDAVKKLQVGHGEWTDDMVSALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKLLSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNPNCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILERARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNIELNVGSAMACFLALSGADINYANHRGKSPLDLIADGRITHLVKNFAQKFREQVELDSPTVSCSLRRVHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLRQDSTEVECSPSLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQIFV

Target ORF information:

RefSeq Version	XM_012966025.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966025.3

ORF Insert Sequence:

ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA 
CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC 
AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT 
CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT 
GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG 
AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA 
GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT 
GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA 
GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT 
GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT 
CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT 
GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA 
GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG 
GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA 
GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG 
GCTCTAACAA AGCAAAACAG CTTTTGGGTT GGTGATGTTG TGCGTGTGAT GGATAACATG 
GATGCAGTCA AGAAGCTCCA AGTTGGTCAT GGGGAGTGGA CAGATGATAT GGTTTCTGCG 
CTGGGTCAAA TTGGCAAGGT GATCAAAGTT TTTGGGGATG GAGACATGCG AGTTTCTGTT 
GGAGGCCAGT CCTGGACATT CAACCCTGCG TGCCTCACGT CATACCAACG GGAAGAGGAT 
GCTAACCTCA TGACCACTGA AAATGCCAAG GAGTCTAAAA ACACCCTGGT TTCCATATTA 
GAAAAGTTGC TTTCTCAGAA GACTGATTGT GAAAGTCCTA CTTCCTTGGT TATTGAAGCA 
GCCCAGGGCA ATACAGCCAA AGTCAGGGAG ATGCTCCAGA AATACCCCGA CAAGGTGGAT 
ATTAAGAATC AGGGCAGGAC GGCTTTGCAG GTTGCCTCCC ATTTAGGCTA TATGGAGGTG 
GTGAAGGTTT TGCTTCAAGC CAATGCAAAC ATTGACCTCA GGGATGACGA GGGGGACACT 
GCTTTGCATT ATGCTGCTTA TGGAAACCAG GCTGGTGTGG TTAGAGTTTT GCTATCTAAA 
GGAGCCAATG CAGAACTTCT AAACAATGCC AAGTGTACAG CGCTCTACAT TGCAGTTAAC 
AAAGGGTTCA CAGAAGTAGT CCAAGTCCTC TGCAACCCAA ATTGTGCCAT CAACATGCAG 
GATTCCTTTG GGGACACACC TCTGCATTAT GCAATCACCG CAGACTTTAG AAGCATCATT 
GAGATCCTCA CTGAAGTACC CAATATAGAT TTCACTGTGC AGAACAATCA AGGGTTTAAT 
CTACTGCATC ACTCAGCTCT CAAGGGAAAT GTCCTGGCTG TAAGCAAGAT CCTGGAGAGA 
GCAAGACAGC TTGTGGACTC TAAAAAAGAA GATGGTTTCA CTGCCCTTCA TCTTGCAACA 
CTGAACAACC ACCAGGAGGT GGTAGAAATA TTGATCAAAG AGGGCCGCTG TGATGTCAAT 
CTCAGGAATA ACAGAAACCA GACCCCTCTG CATTTGGCTG TCGCCCAGGG CCACATCAGC 
TTGGTGCATC TCTTGGTGAC TGAGGGTGCC GATGTGAATG CAGAGGATGA GGATGGAGAT 
ACCCCAATGC ACATAGTATT TGTGCGACAG CATCTCAAAT CCATTGACTC TCAGCAAGAA 
GGCAATGGGT CATCACTACT CACAAAGTTG GAGGCCTCTG GGTTGCCGGG AAATATAGAG 
TTAAATGTTG GTTCAGCCAT GGCTTGCTTT TTAGCTCTGT CTGGAGCTGA CATTAACTAT 
GCCAATCACC GTGGGAAGTC TCCCCTGGAC CTGATCGCAG ATGGAAGGAT AACGCACCTT 
GTGAAAAACT TTGCACAGAA ATTCAGAGAA CAAGTGGAGT TAGACTCACC AACCGTCAGT 
TGCAGCCTCC GCAGAGTCCA CACTACACCT AATACTATGA CTAACCTTAG CCCCTGTAGT 
GCCGCCAGTC CTGCAGAGTG TCTAGTATGC TCTGAGCTGG CCGTACTAGT CAGTTTCTTC 
CCCTGCCAAC ACAGCATAGT ATGTGAAGAA TGTTCCAGAC GAATGAAGAA GTGCATCAAA 
TGTCAAGTGT GCATAGCAAG AAAGCTTAGG CAAGACAGTA CTGAAGTTGA ATGCAGTCCC 
AGTTTAGAAG TGTCAGACCA GAAGAAAGTA ATGGAGGAGC TCCAGAATCG ATACCGCCAA 
ATGGAGGAAA GGATCACCTG TCCTATTTGT ATCGACAATC ACATCAAGCT GGTGTTCCAG 
TGTGGACATG GGTCTTGTAC TGAATGCAGC ACTTCCCTGA CTGCCTGTCC CATATGCAGG 
CAGCTTATCA GAGAGAGGAT ACAGATATTT GTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mib2 ( XM_012966028.3 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_012821482.1 Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X3, mRNA.

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CloneID	OXa31375
Clone ID Related Accession (Same CDS sequence)	XM_012966028.3 , XM_012966028.2
Accession Version	XM_012966028.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2751bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	E3 ubiquitin-protein ligase MIB2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030683.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012966028.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA 
CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC 
AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT 
CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT 
GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG 
AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA 
GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT 
GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA 
GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT 
GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT 
CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT 
GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA 
GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG 
GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA 
GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG 
GCTCTAACAA AGGCGCTGGG TCAAATTGGC AAGGTGATCA AAGTTTTTGG GGATGGAGAC 
ATGCGAGTTT CTGTTGGAGG CCAGTCCTGG ACATTCAACC CTGCGTGCCT CACGTCATAC 
CAACGGGAAG AGGATGCTAA CCTCATGACC ACTGAAAATG CCAAGGAGTC TAAAAACACC 
CTGGTTTCCA TATTAGAAAA GTTGCTTTCT CAGAAGACTG ATTGTGAAAG TCCTACTTCC 
TTGGTTATTG AAGCAGCCCA GGGCAATACA GCCAAAGTCA GGGAGATGCT CCAGAAATAC 
CCCGACAAGG TGGATATTAA GAATCAGGGC AGGACGGCTT TGCAGGTTGC CTCCCATTTA 
GGCTATATGG AGGTGGTGAA GGTTTTGCTT CAAGCCAATG CAAACATTGA CCTCAGGGAT 
GACGAGGGGG ACACTGCTTT GCATTATGCT GCTTATGGAA ACCAGGCTGG TGTGGTTAGA 
GTTTTGCTAT CTAAAGGAGC CAATGCAGAA CTTCTAAACA ATGCCAAGTG TACAGCGCTC 
TACATTGCAG TTAACAAAGG GTTCACAGAA GTAGTCCAAG TCCTCTGCAA CCCAAATTGT 
GCCATCAACA TGCAGGATTC CTTTGGGGAC ACACCTCTGC ATTATGCAAT CACCGCAGAC 
TTTAGAAGCA TCATTGAGAT CCTCACTGAA GTACCCAATA TAGATTTCAC TGTGCAGAAC 
AATCAAGGGT TTAATCTACT GCATCACTCA GCTCTCAAGG GAAATGTCCT GGCTGTAAGC 
AAGATCCTGG AGAGAGCAAG ACAGCTTGTG GACTCTAAAA AAGAAGATGG TTTCACTGCC 
CTTCATCTTG CAACACTGAA CAACCACCAG GAGGTGGTAG AAATATTGAT CAAAGAGGGC 
CGCTGTGATG TCAATCTCAG GAATAACAGA AACCAGACCC CTCTGCATTT GGCTGTCGCC 
CAGGGCCACA TCAGCTTGGT GCATCTCTTG GTGACTGAGG GTGCCGATGT GAATGCAGAG 
GATGAGGATG GAGATACCCC AATGCACATA GTATTTGTGC GACAGCATCT CAAATCCATT 
GACTCTCAGC AAGAAGGCAA TGGGTCATCA CTACTCACAA AGTTGGAGGC CTCTGGGTTG 
CCGGGAAATA TAGAGTTAAA TGTTGGTTCA GCCATGGCTT GCTTTTTAGC TCTGTCTGGA 
GCTGACATTA ACTATGCCAA TCACCGTGGG AAGTCTCCCC TGGACCTGAT CGCAGATGGA 
AGGATAACGC ACCTTGTGAA AAACTTTGCA CAGAAATTCA GAGAACAAGT GGAGTTAGAC 
TCACCAACCG TCAGTTGCAG CCTCCGCAGA GTCCACACTA CACCTAATAC TATGACTAAC 
CTTAGCCCCT GTAGTGCCGC CAGTCCTGCA GAGTGTCTAG TATGCTCTGA GCTGGCCGTA 
CTAGTCAGTT TCTTCCCCTG CCAACACAGC ATAGTATGTG AAGAATGTTC CAGACGAATG 
AAGAAGTGCA TCAAATGTCA AGTGTGCATA GCAAGAAAGC TTAGGCAAGA CAGTACTGAA 
GTTGAATGCA GTCCCAGTTT AGAAGTGTCA GACCAGAAGA AAGTAATGGA GGAGCTCCAG 
AATCGATACC GCCAAATGGA GGAAAGGATC ACCTGTCCTA TTTGTATCGA CAATCACATC 
AAGCTGGTGT TCCAGTGTGG ACATGGGTCT TGTACTGAAT GCAGCACTTC CCTGACTGCC 
TGTCCCATAT GCAGGCAGCT TATCAGAGAG AGGATACAGA TATTTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012821482.1
CDS	471..3221
Translation	MDWDNSAAMQVGMRVVRGIDWKWSNQDNGEGSMGTVVEIGRQGSPTTPDKTVVVQWDHGTRTNYRTGFQGAYDLLLYDNAQTGVRHPNIICDCCKKHGIRGMRWKCKVCFDYDLCTQCYMNNKHDLSHIFERYETADSRPVILSTRQGLPRVVLKGIFQGAKVVRGPDWEWGNQDGGEGKVGRVVDIRGWDVETGRSVASVTWADGTTNVYRVGHKGKVDLKCITDAPGGHYYRDHLPKLGKPAELQRKESSERHPFQHGDKVKCLLDVEILREMQEGHGGWNPKMAEFIGQTGTVHRITERGDVRVQYNSETRWTFHPGALTKALGQIGKVIKVFGDGDMRVSVGGQSWTFNPACLTSYQREEDANLMTTENAKESKNTLVSILEKLLSQKTDCESPTSLVIEAAQGNTAKVREMLQKYPDKVDIKNQGRTALQVASHLGYMEVVKVLLQANANIDLRDDEGDTALHYAAYGNQAGVVRVLLSKGANAELLNNAKCTALYIAVNKGFTEVVQVLCNPNCAINMQDSFGDTPLHYAITADFRSIIEILTEVPNIDFTVQNNQGFNLLHHSALKGNVLAVSKILERARQLVDSKKEDGFTALHLATLNNHQEVVEILIKEGRCDVNLRNNRNQTPLHLAVAQGHISLVHLLVTEGADVNAEDEDGDTPMHIVFVRQHLKSIDSQQEGNGSSLLTKLEASGLPGNIELNVGSAMACFLALSGADINYANHRGKSPLDLIADGRITHLVKNFAQKFREQVELDSPTVSCSLRRVHTTPNTMTNLSPCSAASPAECLVCSELAVLVSFFPCQHSIVCEECSRRMKKCIKCQVCIARKLRQDSTEVECSPSLEVSDQKKVMEELQNRYRQMEERITCPICIDNHIKLVFQCGHGSCTECSTSLTACPICRQLIRERIQIFV

Target ORF information:

RefSeq Version	XM_012966028.3
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis mindbomb E3 ubiquitin protein ligase 2 (mib2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012966028.3

ORF Insert Sequence:

ATGGACTGGG ACAACTCGGC TGCAATGCAA GTGGGAATGC GTGTGGTGAG AGGTATAGAC 
TGGAAATGGT CTAACCAGGA CAATGGTGAA GGCAGCATGG GCACTGTGGT AGAGATCGGA 
CGTCAGGGAA GCCCTACCAC ACCTGACAAA ACAGTAGTTG TTCAATGGGA TCATGGCACC 
AGGACTAATT ATCGGACTGG CTTCCAAGGA GCTTATGACC TTCTGCTGTA TGACAATGCT 
CAGACAGGTG TCCGCCATCC CAACATCATC TGTGACTGCT GCAAGAAGCA TGGAATCCGT 
GGCATGCGCT GGAAGTGCAA AGTCTGTTTC GACTATGATC TGTGCACACA GTGCTATATG 
AATAACAAGC ACGATTTGTC CCATATCTTC GAGAGATATG AGACCGCCGA TTCCCGACCA 
GTCATACTGA GCACTCGGCA AGGCTTGCCC CGGGTTGTGT TAAAAGGAAT CTTCCAAGGT 
GCAAAAGTGG TGCGTGGTCC TGACTGGGAA TGGGGAAACC AAGATGGTGG GGAAGGTAAA 
GTTGGCCGAG TCGTTGATAT CCGTGGATGG GATGTAGAGA CTGGCCGCAG TGTGGCTAGT 
GTAACATGGG CTGATGGAAC CACTAATGTG TACAGAGTTG GCCACAAGGG AAAGGTTGAT 
CTTAAGTGCA TTACTGATGC TCCGGGAGGT CATTATTACA GGGATCACTT GCCCAAGCTT 
GGTAAACCTG CTGAGTTGCA AAGAAAAGAA AGTTCAGAAA GGCACCCGTT TCAGCATGGA 
GACAAAGTGA AGTGTCTTTT AGATGTTGAG ATATTACGAG AGATGCAAGA AGGACATGGG 
GGATGGAACC CTAAAATGGC AGAGTTTATA GGTCAGACTG GAACAGTACA TAGGATTACA 
GAGCGAGGTG ATGTCAGAGT TCAGTACAAC AGTGAGACAC GATGGACATT TCATCCCGGG 
GCTCTAACAA AGGCGCTGGG TCAAATTGGC AAGGTGATCA AAGTTTTTGG GGATGGAGAC 
ATGCGAGTTT CTGTTGGAGG CCAGTCCTGG ACATTCAACC CTGCGTGCCT CACGTCATAC 
CAACGGGAAG AGGATGCTAA CCTCATGACC ACTGAAAATG CCAAGGAGTC TAAAAACACC 
CTGGTTTCCA TATTAGAAAA GTTGCTTTCT CAGAAGACTG ATTGTGAAAG TCCTACTTCC 
TTGGTTATTG AAGCAGCCCA GGGCAATACA GCCAAAGTCA GGGAGATGCT CCAGAAATAC 
CCCGACAAGG TGGATATTAA GAATCAGGGC AGGACGGCTT TGCAGGTTGC CTCCCATTTA 
GGCTATATGG AGGTGGTGAA GGTTTTGCTT CAAGCCAATG CAAACATTGA CCTCAGGGAT 
GACGAGGGGG ACACTGCTTT GCATTATGCT GCTTATGGAA ACCAGGCTGG TGTGGTTAGA 
GTTTTGCTAT CTAAAGGAGC CAATGCAGAA CTTCTAAACA ATGCCAAGTG TACAGCGCTC 
TACATTGCAG TTAACAAAGG GTTCACAGAA GTAGTCCAAG TCCTCTGCAA CCCAAATTGT 
GCCATCAACA TGCAGGATTC CTTTGGGGAC ACACCTCTGC ATTATGCAAT CACCGCAGAC 
TTTAGAAGCA TCATTGAGAT CCTCACTGAA GTACCCAATA TAGATTTCAC TGTGCAGAAC 
AATCAAGGGT TTAATCTACT GCATCACTCA GCTCTCAAGG GAAATGTCCT GGCTGTAAGC 
AAGATCCTGG AGAGAGCAAG ACAGCTTGTG GACTCTAAAA AAGAAGATGG TTTCACTGCC 
CTTCATCTTG CAACACTGAA CAACCACCAG GAGGTGGTAG AAATATTGAT CAAAGAGGGC 
CGCTGTGATG TCAATCTCAG GAATAACAGA AACCAGACCC CTCTGCATTT GGCTGTCGCC 
CAGGGCCACA TCAGCTTGGT GCATCTCTTG GTGACTGAGG GTGCCGATGT GAATGCAGAG 
GATGAGGATG GAGATACCCC AATGCACATA GTATTTGTGC GACAGCATCT CAAATCCATT 
GACTCTCAGC AAGAAGGCAA TGGGTCATCA CTACTCACAA AGTTGGAGGC CTCTGGGTTG 
CCGGGAAATA TAGAGTTAAA TGTTGGTTCA GCCATGGCTT GCTTTTTAGC TCTGTCTGGA 
GCTGACATTA ACTATGCCAA TCACCGTGGG AAGTCTCCCC TGGACCTGAT CGCAGATGGA 
AGGATAACGC ACCTTGTGAA AAACTTTGCA CAGAAATTCA GAGAACAAGT GGAGTTAGAC 
TCACCAACCG TCAGTTGCAG CCTCCGCAGA GTCCACACTA CACCTAATAC TATGACTAAC 
CTTAGCCCCT GTAGTGCCGC CAGTCCTGCA GAGTGTCTAG TATGCTCTGA GCTGGCCGTA 
CTAGTCAGTT TCTTCCCCTG CCAACACAGC ATAGTATGTG AAGAATGTTC CAGACGAATG 
AAGAAGTGCA TCAAATGTCA AGTGTGCATA GCAAGAAAGC TTAGGCAAGA CAGTACTGAA 
GTTGAATGCA GTCCCAGTTT AGAAGTGTCA GACCAGAAGA AAGTAATGGA GGAGCTCCAG 
AATCGATACC GCCAAATGGA GGAAAGGATC ACCTGTCCTA TTTGTATCGA CAATCACATC 
AAGCTGGTGT TCCAGTGTGG ACATGGGTCT TGTACTGAAT GCAGCACTTC CCTGACTGCC 
TGTCCCATAT GCAGGCAGCT TATCAGAGAG AGGATACAGA TATTTGTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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mib2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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