MIB2 cDNA ORF clone, Bos taurus(cattle)

The following MIB2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIB2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa27381 XM_010813440.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa223976 XM_024976796.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa223977 XM_024976797.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa223978 XM_024976799.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa223978 XM_024976798.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa223979 XM_024976800.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa223980 XM_024976801.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa223981 XM_024976802.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa223981 XM_024976803.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa223982 XM_024976804.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa223983 XM_024976805.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa223984 XM_024976806.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OBa94880 XM_015466875.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa94881 XM_015466876.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa94882 XM_015466877.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa94883 XM_015466878.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa94884 XM_015466879.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa94885 XM_015466880.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa94886 XM_015466881.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa94887 XM_015466882.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa94888 XM_015466883.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
OBa94878 XM_015466873.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OBa94879 XM_015466874.1
Latest version!
Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa27381
    Clone ID Related Accession (Same CDS sequence) XM_010813440.1
    Accession Version XM_010813440.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2859bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104439.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGACCTC
    AGCCCCGAGC CCCCGCCCCT GACCTGGACC TCTTCCGCAG ACGGCACGGA GGTGGTCAGC
    GCAGCCCCCG CGCCCGGCCC ACCGCGGCAG TTGGTGGAGG AGCTGCAGAG CCGCTACAGG
    CAGATGGAGG AGCGCATCAC CTGTCCGATT TGCATCGACA GCCACATCCG CCTCGTGTTC
    CAGTGCGGCC ACGGCGCGTG CGCGCCCTGT GGCGCGGCGC TCAGCGCCTG CCCGATCTGC
    CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010811742.1
    CDS1594..4452
    Translation

    Target ORF information:

    RefSeq Version XM_010813440.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010813440.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGACCTC
    AGCCCCGAGC CCCCGCCCCT GACCTGGACC TCTTCCGCAG ACGGCACGGA GGTGGTCAGC
    GCAGCCCCCG CGCCCGGCCC ACCGCGGCAG TTGGTGGAGG AGCTGCAGAG CCGCTACAGG
    CAGATGGAGG AGCGCATCAC CTGTCCGATT TGCATCGACA GCCACATCCG CCTCGTGTTC
    CAGTGCGGCC ACGGCGCGTG CGCGCCCTGT GGCGCGGCGC TCAGCGCCTG CCCGATCTGC
    CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223976
    Clone ID Related Accession (Same CDS sequence) XM_024976796.1
    Accession Version XM_024976796.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2925bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC
    GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA
    GACCTCAGCC CCGAGCCCCC GCCCCTGACC TGGACCTCTT CCGCAGACGG CACGGAGGTG
    GTCAGCGCAG CCCCCGCGCC CGGCCCACCG CGGCAGTTGG TGGAGGAGCT GCAGAGCCGC
    TACAGGCAGA TGGAGGAGCG CATCACCTGT CCGATTTGCA TCGACAGCCA CATCCGCCTC
    GTGTTCCAGT GCGGCCACGG CGCGTGCGCG CCCTGTGGCG CGGCGCTCAG CGCCTGCCCG
    ATCTGCCGCC AGCCCATCCG CGACCGCATC CAGATCTTCG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832564.1
    CDS455..3379
    Translation

    Target ORF information:

    RefSeq Version XM_024976796.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976796.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC
    GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA
    GACCTCAGCC CCGAGCCCCC GCCCCTGACC TGGACCTCTT CCGCAGACGG CACGGAGGTG
    GTCAGCGCAG CCCCCGCGCC CGGCCCACCG CGGCAGTTGG TGGAGGAGCT GCAGAGCCGC
    TACAGGCAGA TGGAGGAGCG CATCACCTGT CCGATTTGCA TCGACAGCCA CATCCGCCTC
    GTGTTCCAGT GCGGCCACGG CGCGTGCGCG CCCTGTGGCG CGGCGCTCAG CGCCTGCCCG
    ATCTGCCGCC AGCCCATCCG CGACCGCATC CAGATCTTCG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223977
    Clone ID Related Accession (Same CDS sequence) XM_024976797.1
    Accession Version XM_024976797.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2913bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC
    GTGCGTGTGC AGTTCAGCCA CAAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG
    CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ATGACCTTGA CACGGTGAAG
    CGGCTGCAGG CTGGCCATGG GGAATGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT
    GGGAGAGTGC TGAAGGTTTT CGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG
    TGGACCTTTA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC
    GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGCG TTGTCTTGGA CAAGCTTCGA
    GCCCAGAAGA GTGACCTGGA GCACCCAGGG AAGCTGGTGG TGGAGGTGGC TCTGGGCAAT
    GTGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG
    GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGCCAGG TGGAGCTGGT GCGGCTGCTG
    CTGCAGGCTC AGGCGGGCGT TGACCTGCCG GATGATGAGG GCAACACGGC ACTGCACTAC
    GCAGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCAGG TTGTGGGGCC
    AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CCGTGCAGAG GGGCTTCCTG
    GAAGTGGTGA AGGTCCTGTG TGAGCGTGGC TGTGACGTCA ACCTGCCTGA TGCCCATGCT
    GATACACCCC TGCACTGCGC CATCTCAGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC
    CTCACCGAGG TGCCAGGCAT CGATGTCACT GCCACCAACA GCCAGGGCTT CACTCTGCTG
    CACCATGCGT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC ACGGGCACGG
    CAGCTGGTGG ACGCCAAGAA GGAGGATGGT TTTACGGCAC TGCACCTGGC CGCCTTCAAC
    AACCATGGGG AGGTGGCCCA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC
    AACCGTAAGC TGCAGTCCCC ACTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG
    CCACTGCTGG TGGATGCGGG CTGCAGCGTC AACGCCGAGG ATGAGGAGGG GGACACGGCT
    CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG CAGCTGATGG GAGCGCAGGG
    GACCCGGGTC CCCTGCAGCT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC GAGCAGTGCA
    GAGCTGACGG CAGGCGCGGC TATGGCCTAC TACTTAGCGC TGGAGGGCGC CGACCTGAGC
    TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA
    GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTGGAGGCAG TGGCAGCGGG
    GGCTCGGCCC AGGGTCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTAAC CAACCTGCAT
    GTGGCCGCGC CGACGGGCCC CGAGGCCGCT GAGTGCCTAG TGTGCTCGGA GCTGGCGCTG
    CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGAGTGCGC GCGCAGGATG
    AAGAAATGCA TCAGGTGCCA GGTGGTCATC AGCAAGAAGC TGCGCCCAGA CCTCAGCCCC
    GAGCCCCCGC CCCTGACCTG GACCTCTTCC GCAGACGGCA CGGAGGTGGT CAGCGCAGCC
    CCCGCGCCCG GCCCACCGCG GCAGTTGGTG GAGGAGCTGC AGAGCCGCTA CAGGCAGATG
    GAGGAGCGCA TCACCTGTCC GATTTGCATC GACAGCCACA TCCGCCTCGT GTTCCAGTGC
    GGCCACGGCG CGTGCGCGCC CTGTGGCGCG GCGCTCAGCG CCTGCCCGAT CTGCCGCCAG
    CCCATCCGCG ACCGCATCCA GATCTTCGTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832565.1
    CDS1594..4506
    Translation

    Target ORF information:

    RefSeq Version XM_024976797.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976797.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC
    GTGCGTGTGC AGTTCAGCCA CAAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG
    CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ATGACCTTGA CACGGTGAAG
    CGGCTGCAGG CTGGCCATGG GGAATGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT
    GGGAGAGTGC TGAAGGTTTT CGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG
    TGGACCTTTA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC
    GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGCG TTGTCTTGGA CAAGCTTCGA
    GCCCAGAAGA GTGACCTGGA GCACCCAGGG AAGCTGGTGG TGGAGGTGGC TCTGGGCAAT
    GTGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG
    GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGCCAGG TGGAGCTGGT GCGGCTGCTG
    CTGCAGGCTC AGGCGGGCGT TGACCTGCCG GATGATGAGG GCAACACGGC ACTGCACTAC
    GCAGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCAGG TTGTGGGGCC
    AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CCGTGCAGAG GGGCTTCCTG
    GAAGTGGTGA AGGTCCTGTG TGAGCGTGGC TGTGACGTCA ACCTGCCTGA TGCCCATGCT
    GATACACCCC TGCACTGCGC CATCTCAGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC
    CTCACCGAGG TGCCAGGCAT CGATGTCACT GCCACCAACA GCCAGGGCTT CACTCTGCTG
    CACCATGCGT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC ACGGGCACGG
    CAGCTGGTGG ACGCCAAGAA GGAGGATGGT TTTACGGCAC TGCACCTGGC CGCCTTCAAC
    AACCATGGGG AGGTGGCCCA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC
    AACCGTAAGC TGCAGTCCCC ACTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG
    CCACTGCTGG TGGATGCGGG CTGCAGCGTC AACGCCGAGG ATGAGGAGGG GGACACGGCT
    CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG CAGCTGATGG GAGCGCAGGG
    GACCCGGGTC CCCTGCAGCT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC GAGCAGTGCA
    GAGCTGACGG CAGGCGCGGC TATGGCCTAC TACTTAGCGC TGGAGGGCGC CGACCTGAGC
    TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA
    GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTGGAGGCAG TGGCAGCGGG
    GGCTCGGCCC AGGGTCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTAAC CAACCTGCAT
    GTGGCCGCGC CGACGGGCCC CGAGGCCGCT GAGTGCCTAG TGTGCTCGGA GCTGGCGCTG
    CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGAGTGCGC GCGCAGGATG
    AAGAAATGCA TCAGGTGCCA GGTGGTCATC AGCAAGAAGC TGCGCCCAGA CCTCAGCCCC
    GAGCCCCCGC CCCTGACCTG GACCTCTTCC GCAGACGGCA CGGAGGTGGT CAGCGCAGCC
    CCCGCGCCCG GCCCACCGCG GCAGTTGGTG GAGGAGCTGC AGAGCCGCTA CAGGCAGATG
    GAGGAGCGCA TCACCTGTCC GATTTGCATC GACAGCCACA TCCGCCTCGT GTTCCAGTGC
    GGCCACGGCG CGTGCGCGCC CTGTGGCGCG GCGCTCAGCG CCTGCCCGAT CTGCCGCCAG
    CCCATCCGCG ACCGCATCCA GATCTTCGTG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223978
    Clone ID Related Accession (Same CDS sequence) XM_024976798.1 , XM_024976799.1
    Accession Version XM_024976798.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2880bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC
    GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA
    GACGGCACGG AGGTGGTCAG CGCAGCCCCC GCGCCCGGCC CACCGCGGCA GTTGGTGGAG
    GAGCTGCAGA GCCGCTACAG GCAGATGGAG GAGCGCATCA CCTGTCCGAT TTGCATCGAC
    AGCCACATCC GCCTCGTGTT CCAGTGCGGC CACGGCGCGT GCGCGCCCTG TGGCGCGGCG
    CTCAGCGCCT GCCCGATCTG CCGCCAGCCC ATCCGCGACC GCATCCAGAT CTTCGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832566.1
    CDS453..3332
    Translation

    Target ORF information:

    RefSeq Version XM_024976798.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976798.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC
    GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA
    GACGGCACGG AGGTGGTCAG CGCAGCCCCC GCGCCCGGCC CACCGCGGCA GTTGGTGGAG
    GAGCTGCAGA GCCGCTACAG GCAGATGGAG GAGCGCATCA CCTGTCCGAT TTGCATCGAC
    AGCCACATCC GCCTCGTGTT CCAGTGCGGC CACGGCGCGT GCGCGCCCTG TGGCGCGGCG
    CTCAGCGCCT GCCCGATCTG CCGCCAGCCC ATCCGCGACC GCATCCAGAT CTTCGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223978
    Clone ID Related Accession (Same CDS sequence) XM_024976798.1 , XM_024976799.1
    Accession Version XM_024976799.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2880bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC
    GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA
    GACGGCACGG AGGTGGTCAG CGCAGCCCCC GCGCCCGGCC CACCGCGGCA GTTGGTGGAG
    GAGCTGCAGA GCCGCTACAG GCAGATGGAG GAGCGCATCA CCTGTCCGAT TTGCATCGAC
    AGCCACATCC GCCTCGTGTT CCAGTGCGGC CACGGCGCGT GCGCGCCCTG TGGCGCGGCG
    CTCAGCGCCT GCCCGATCTG CCGCCAGCCC ATCCGCGACC GCATCCAGAT CTTCGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832567.1
    CDS385..3264
    Translation

    Target ORF information:

    RefSeq Version XM_024976799.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976799.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC
    GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTGCGCCCA
    GACGGCACGG AGGTGGTCAG CGCAGCCCCC GCGCCCGGCC CACCGCGGCA GTTGGTGGAG
    GAGCTGCAGA GCCGCTACAG GCAGATGGAG GAGCGCATCA CCTGTCCGAT TTGCATCGAC
    AGCCACATCC GCCTCGTGTT CCAGTGCGGC CACGGCGCGT GCGCGCCCTG TGGCGCGGCG
    CTCAGCGCCT GCCCGATCTG CCGCCAGCCC ATCCGCGACC GCATCCAGAT CTTCGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223979
    Clone ID Related Accession (Same CDS sequence) XM_024976800.1
    Accession Version XM_024976800.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2868bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC
    GTGCGTGTGC AGTTCAGCCA CAAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG
    CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ATGACCTTGA CACGGTGAAG
    CGGCTGCAGG CTGGCCATGG GGAATGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT
    GGGAGAGTGC TGAAGGTTTT CGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG
    TGGACCTTTA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC
    GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGCG TTGTCTTGGA CAAGCTTCGA
    GCCCAGAAGA GTGACCTGGA GCACCCAGGG AAGCTGGTGG TGGAGGTGGC TCTGGGCAAT
    GTGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG
    GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGCCAGG TGGAGCTGGT GCGGCTGCTG
    CTGCAGGCTC AGGCGGGCGT TGACCTGCCG GATGATGAGG GCAACACGGC ACTGCACTAC
    GCAGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCAGG TTGTGGGGCC
    AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CCGTGCAGAG GGGCTTCCTG
    GAAGTGGTGA AGGTCCTGTG TGAGCGTGGC TGTGACGTCA ACCTGCCTGA TGCCCATGCT
    GATACACCCC TGCACTGCGC CATCTCAGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC
    CTCACCGAGG TGCCAGGCAT CGATGTCACT GCCACCAACA GCCAGGGCTT CACTCTGCTG
    CACCATGCGT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC ACGGGCACGG
    CAGCTGGTGG ACGCCAAGAA GGAGGATGGT TTTACGGCAC TGCACCTGGC CGCCTTCAAC
    AACCATGGGG AGGTGGCCCA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC
    AACCGTAAGC TGCAGTCCCC ACTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG
    CCACTGCTGG TGGATGCGGG CTGCAGCGTC AACGCCGAGG ATGAGGAGGG GGACACGGCT
    CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG CAGCTGATGG GAGCGCAGGG
    GACCCGGGTC CCCTGCAGCT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC GAGCAGTGCA
    GAGCTGACGG CAGGCGCGGC TATGGCCTAC TACTTAGCGC TGGAGGGCGC CGACCTGAGC
    TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA
    GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTGGAGGCAG TGGCAGCGGG
    GGCTCGGCCC AGGGTCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTAAC CAACCTGCAT
    GTGGCCGCGC CGACGGGCCC CGAGGCCGCT GAGTGCCTAG TGTGCTCGGA GCTGGCGCTG
    CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGAGTGCGC GCGCAGGATG
    AAGAAATGCA TCAGGTGCCA GGTGGTCATC AGCAAGAAGC TGCGCCCAGA CGGCACGGAG
    GTGGTCAGCG CAGCCCCCGC GCCCGGCCCA CCGCGGCAGT TGGTGGAGGA GCTGCAGAGC
    CGCTACAGGC AGATGGAGGA GCGCATCACC TGTCCGATTT GCATCGACAG CCACATCCGC
    CTCGTGTTCC AGTGCGGCCA CGGCGCGTGC GCGCCCTGTG GCGCGGCGCT CAGCGCCTGC
    CCGATCTGCC GCCAGCCCAT CCGCGACCGC ATCCAGATCT TCGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832568.1
    CDS460..3327
    Translation

    Target ORF information:

    RefSeq Version XM_024976800.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976800.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC
    GTGCGTGTGC AGTTCAGCCA CAAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG
    CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ATGACCTTGA CACGGTGAAG
    CGGCTGCAGG CTGGCCATGG GGAATGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT
    GGGAGAGTGC TGAAGGTTTT CGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG
    TGGACCTTTA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC
    GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGCG TTGTCTTGGA CAAGCTTCGA
    GCCCAGAAGA GTGACCTGGA GCACCCAGGG AAGCTGGTGG TGGAGGTGGC TCTGGGCAAT
    GTGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG
    GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGCCAGG TGGAGCTGGT GCGGCTGCTG
    CTGCAGGCTC AGGCGGGCGT TGACCTGCCG GATGATGAGG GCAACACGGC ACTGCACTAC
    GCAGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCAGG TTGTGGGGCC
    AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CCGTGCAGAG GGGCTTCCTG
    GAAGTGGTGA AGGTCCTGTG TGAGCGTGGC TGTGACGTCA ACCTGCCTGA TGCCCATGCT
    GATACACCCC TGCACTGCGC CATCTCAGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC
    CTCACCGAGG TGCCAGGCAT CGATGTCACT GCCACCAACA GCCAGGGCTT CACTCTGCTG
    CACCATGCGT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC ACGGGCACGG
    CAGCTGGTGG ACGCCAAGAA GGAGGATGGT TTTACGGCAC TGCACCTGGC CGCCTTCAAC
    AACCATGGGG AGGTGGCCCA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC
    AACCGTAAGC TGCAGTCCCC ACTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG
    CCACTGCTGG TGGATGCGGG CTGCAGCGTC AACGCCGAGG ATGAGGAGGG GGACACGGCT
    CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG CAGCTGATGG GAGCGCAGGG
    GACCCGGGTC CCCTGCAGCT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC GAGCAGTGCA
    GAGCTGACGG CAGGCGCGGC TATGGCCTAC TACTTAGCGC TGGAGGGCGC CGACCTGAGC
    TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA
    GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTGGAGGCAG TGGCAGCGGG
    GGCTCGGCCC AGGGTCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTAAC CAACCTGCAT
    GTGGCCGCGC CGACGGGCCC CGAGGCCGCT GAGTGCCTAG TGTGCTCGGA GCTGGCGCTG
    CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGAGTGCGC GCGCAGGATG
    AAGAAATGCA TCAGGTGCCA GGTGGTCATC AGCAAGAAGC TGCGCCCAGA CGGCACGGAG
    GTGGTCAGCG CAGCCCCCGC GCCCGGCCCA CCGCGGCAGT TGGTGGAGGA GCTGCAGAGC
    CGCTACAGGC AGATGGAGGA GCGCATCACC TGTCCGATTT GCATCGACAG CCACATCCGC
    CTCGTGTTCC AGTGCGGCCA CGGCGCGTGC GCGCCCTGTG GCGCGGCGCT CAGCGCCTGC
    CCGATCTGCC GCCAGCCCAT CCGCGACCGC ATCCAGATCT TCGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223980
    Clone ID Related Accession (Same CDS sequence) XM_024976801.1
    Accession Version XM_024976801.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2817bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT
    ATCGATGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAATG GACCGATGAC
    ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTCGGAGA CGGGAACGTG
    GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTTAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG
    AGCGTTGTCT TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAAGCTG
    GTGGTGGAGG TGGCTCTGGG CAATGTGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA
    GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGC
    CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCGGATGAT
    GAGGGCAACA CGGCACTGCA CTACGCAGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG
    CTCTTGAGCT CAGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT
    GTGGCCGTGC AGAGGGGCTT CCTGGAAGTG GTGAAGGTCC TGTGTGAGCG TGGCTGTGAC
    GTCAACCTGC CTGATGCCCA TGCTGATACA CCCCTGCACT GCGCCATCTC AGCGGGCACT
    GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGATGT CACTGCCACC
    AACAGCCAGG GCTTCACTCT GCTGCACCAT GCGTCCCTCA AAGGCCACAC ACTAGCTGTC
    AGGAGGATTC TGGCACGGGC ACGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGTTTTACG
    GCACTGCACC TGGCCGCCTT CAACAACCAT GGGGAGGTGG CCCAGGTTCT CATCCAAGAG
    GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCACTGCA CCTGGCTGTG
    CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGATG CGGGCTGCAG CGTCAACGCC
    GAGGATGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT
    CTGGCAGCTG ATGGGAGCGC AGGGGACCCG GGTCCCCTGC AGCTGCTGTC CAGGCTACAG
    GCCTCGGGCC TTCCGAGCAG TGCAGAGCTG ACGGCAGGCG CGGCTATGGC CTACTACTTA
    GCGCTGGAGG GCGCCGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG
    GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG
    CAGGCTGGAG GCAGTGGCAG CGGGGGCTCG GCCCAGGGTC CAAGACTGGT GCTCAGTGCT
    CCGAACACTG TAACCAACCT GCATGTGGCC GCGCCGACGG GCCCCGAGGC CGCTGAGTGC
    CTAGTGTGCT CGGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG
    TGCGAGGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG
    AAGCTGCGCC CAGACCTCAG CCCCGAGCCC CCGCCCCTGA CCTGGACCTC TTCCGCAGAC
    GGCACGGAGG TGGTCAGCGC AGCCCCCGCG CCCGGCCCAC CGCGGCAGTT GGTGGAGGAG
    CTGCAGAGCC GCTACAGGCA GATGGAGGAG CGCATCACCT GTCCGATTTG CATCGACAGC
    CACATCCGCC TCGTGTTCCA GTGCGGCCAC GGCGCGTGCG CGCCCTGTGG CGCGGCGCTC
    AGCGCCTGCC CGATCTGCCG CCAGCCCATC CGCGACCGCA TCCAGATCTT CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832569.1
    CDS1594..4410
    Translation

    Target ORF information:

    RefSeq Version XM_024976801.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976801.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT
    ATCGATGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAATG GACCGATGAC
    ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTCGGAGA CGGGAACGTG
    GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTTAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG
    AGCGTTGTCT TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAAGCTG
    GTGGTGGAGG TGGCTCTGGG CAATGTGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA
    GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGC
    CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCGGATGAT
    GAGGGCAACA CGGCACTGCA CTACGCAGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG
    CTCTTGAGCT CAGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT
    GTGGCCGTGC AGAGGGGCTT CCTGGAAGTG GTGAAGGTCC TGTGTGAGCG TGGCTGTGAC
    GTCAACCTGC CTGATGCCCA TGCTGATACA CCCCTGCACT GCGCCATCTC AGCGGGCACT
    GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGATGT CACTGCCACC
    AACAGCCAGG GCTTCACTCT GCTGCACCAT GCGTCCCTCA AAGGCCACAC ACTAGCTGTC
    AGGAGGATTC TGGCACGGGC ACGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGTTTTACG
    GCACTGCACC TGGCCGCCTT CAACAACCAT GGGGAGGTGG CCCAGGTTCT CATCCAAGAG
    GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCACTGCA CCTGGCTGTG
    CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGATG CGGGCTGCAG CGTCAACGCC
    GAGGATGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT
    CTGGCAGCTG ATGGGAGCGC AGGGGACCCG GGTCCCCTGC AGCTGCTGTC CAGGCTACAG
    GCCTCGGGCC TTCCGAGCAG TGCAGAGCTG ACGGCAGGCG CGGCTATGGC CTACTACTTA
    GCGCTGGAGG GCGCCGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG
    GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG
    CAGGCTGGAG GCAGTGGCAG CGGGGGCTCG GCCCAGGGTC CAAGACTGGT GCTCAGTGCT
    CCGAACACTG TAACCAACCT GCATGTGGCC GCGCCGACGG GCCCCGAGGC CGCTGAGTGC
    CTAGTGTGCT CGGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG
    TGCGAGGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG
    AAGCTGCGCC CAGACCTCAG CCCCGAGCCC CCGCCCCTGA CCTGGACCTC TTCCGCAGAC
    GGCACGGAGG TGGTCAGCGC AGCCCCCGCG CCCGGCCCAC CGCGGCAGTT GGTGGAGGAG
    CTGCAGAGCC GCTACAGGCA GATGGAGGAG CGCATCACCT GTCCGATTTG CATCGACAGC
    CACATCCGCC TCGTGTTCCA GTGCGGCCAC GGCGCGTGCG CGCCCTGTGG CGCGGCGCTC
    AGCGCCTGCC CGATCTGCCG CCAGCCCATC CGCGACCGCA TCCAGATCTT CGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223981
    Clone ID Related Accession (Same CDS sequence) XM_024976803.1 , XM_024976802.1
    Accession Version XM_024976803.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT
    ATCGATGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAATG GACCGATGAC
    ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTCGGAGA CGGGAACGTG
    GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTTAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG
    AGCGTTGTCT TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAAGCTG
    GTGGTGGAGG TGGCTCTGGG CAATGTGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA
    GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGC
    CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCGGATGAT
    GAGGGCAACA CGGCACTGCA CTACGCAGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG
    CTCTTGAGCT CAGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT
    GTGGCCGTGC AGAGGGGCTT CCTGGAAGTG GTGAAGGTCC TGTGTGAGCG TGGCTGTGAC
    GTCAACCTGC CTGATGCCCA TGCTGATACA CCCCTGCACT GCGCCATCTC AGCGGGCACT
    GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGATGT CACTGCCACC
    AACAGCCAGG GCTTCACTCT GCTGCACCAT GCGTCCCTCA AAGGCCACAC ACTAGCTGTC
    AGGAGGATTC TGGCACGGGC ACGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGTTTTACG
    GCACTGCACC TGGCCGCCTT CAACAACCAT GGGGAGGTGG CCCAGGTTCT CATCCAAGAG
    GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCACTGCA CCTGGCTGTG
    CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGATG CGGGCTGCAG CGTCAACGCC
    GAGGATGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT
    CTGGCAGCTG ATGGGAGCGC AGGGGACCCG GGTCCCCTGC AGCTGCTGTC CAGGCTACAG
    GCCTCGGGCC TTCCGAGCAG TGCAGAGCTG ACGGCAGGCG CGGCTATGGC CTACTACTTA
    GCGCTGGAGG GCGCCGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG
    GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG
    CAGGCTGGAG GCAGTGGCAG CGGGGGCTCG GCCCAGGGTC CAAGACTGGT GCTCAGTGCT
    CCGAACACTG TAACCAACCT GCATGTGGCC GCGCCGACGG GCCCCGAGGC CGCTGAGTGC
    CTAGTGTGCT CGGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG
    TGCGAGGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG
    AAGCTGCGCC CAGACGGCAC GGAGGTGGTC AGCGCAGCCC CCGCGCCCGG CCCACCGCGG
    CAGTTGGTGG AGGAGCTGCA GAGCCGCTAC AGGCAGATGG AGGAGCGCAT CACCTGTCCG
    ATTTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCACGGCGC GTGCGCGCCC
    TGTGGCGCGG CGCTCAGCGC CTGCCCGATC TGCCGCCAGC CCATCCGCGA CCGCATCCAG
    ATCTTCGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832571.1
    CDS350..3121
    Translation

    Target ORF information:

    RefSeq Version XM_024976803.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976803.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT
    ATCGATGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAATG GACCGATGAC
    ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTCGGAGA CGGGAACGTG
    GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTTAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG
    AGCGTTGTCT TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAAGCTG
    GTGGTGGAGG TGGCTCTGGG CAATGTGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA
    GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGC
    CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCGGATGAT
    GAGGGCAACA CGGCACTGCA CTACGCAGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG
    CTCTTGAGCT CAGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT
    GTGGCCGTGC AGAGGGGCTT CCTGGAAGTG GTGAAGGTCC TGTGTGAGCG TGGCTGTGAC
    GTCAACCTGC CTGATGCCCA TGCTGATACA CCCCTGCACT GCGCCATCTC AGCGGGCACT
    GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGATGT CACTGCCACC
    AACAGCCAGG GCTTCACTCT GCTGCACCAT GCGTCCCTCA AAGGCCACAC ACTAGCTGTC
    AGGAGGATTC TGGCACGGGC ACGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGTTTTACG
    GCACTGCACC TGGCCGCCTT CAACAACCAT GGGGAGGTGG CCCAGGTTCT CATCCAAGAG
    GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCACTGCA CCTGGCTGTG
    CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGATG CGGGCTGCAG CGTCAACGCC
    GAGGATGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT
    CTGGCAGCTG ATGGGAGCGC AGGGGACCCG GGTCCCCTGC AGCTGCTGTC CAGGCTACAG
    GCCTCGGGCC TTCCGAGCAG TGCAGAGCTG ACGGCAGGCG CGGCTATGGC CTACTACTTA
    GCGCTGGAGG GCGCCGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG
    GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG
    CAGGCTGGAG GCAGTGGCAG CGGGGGCTCG GCCCAGGGTC CAAGACTGGT GCTCAGTGCT
    CCGAACACTG TAACCAACCT GCATGTGGCC GCGCCGACGG GCCCCGAGGC CGCTGAGTGC
    CTAGTGTGCT CGGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG
    TGCGAGGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG
    AAGCTGCGCC CAGACGGCAC GGAGGTGGTC AGCGCAGCCC CCGCGCCCGG CCCACCGCGG
    CAGTTGGTGG AGGAGCTGCA GAGCCGCTAC AGGCAGATGG AGGAGCGCAT CACCTGTCCG
    ATTTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCACGGCGC GTGCGCGCCC
    TGTGGCGCGG CGCTCAGCGC CTGCCCGATC TGCCGCCAGC CCATCCGCGA CCGCATCCAG
    ATCTTCGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223981
    Clone ID Related Accession (Same CDS sequence) XM_024976803.1 , XM_024976802.1
    Accession Version XM_024976802.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT
    ATCGATGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAATG GACCGATGAC
    ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTCGGAGA CGGGAACGTG
    GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTTAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG
    AGCGTTGTCT TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAAGCTG
    GTGGTGGAGG TGGCTCTGGG CAATGTGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA
    GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGC
    CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCGGATGAT
    GAGGGCAACA CGGCACTGCA CTACGCAGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG
    CTCTTGAGCT CAGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT
    GTGGCCGTGC AGAGGGGCTT CCTGGAAGTG GTGAAGGTCC TGTGTGAGCG TGGCTGTGAC
    GTCAACCTGC CTGATGCCCA TGCTGATACA CCCCTGCACT GCGCCATCTC AGCGGGCACT
    GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGATGT CACTGCCACC
    AACAGCCAGG GCTTCACTCT GCTGCACCAT GCGTCCCTCA AAGGCCACAC ACTAGCTGTC
    AGGAGGATTC TGGCACGGGC ACGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGTTTTACG
    GCACTGCACC TGGCCGCCTT CAACAACCAT GGGGAGGTGG CCCAGGTTCT CATCCAAGAG
    GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCACTGCA CCTGGCTGTG
    CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGATG CGGGCTGCAG CGTCAACGCC
    GAGGATGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT
    CTGGCAGCTG ATGGGAGCGC AGGGGACCCG GGTCCCCTGC AGCTGCTGTC CAGGCTACAG
    GCCTCGGGCC TTCCGAGCAG TGCAGAGCTG ACGGCAGGCG CGGCTATGGC CTACTACTTA
    GCGCTGGAGG GCGCCGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG
    GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG
    CAGGCTGGAG GCAGTGGCAG CGGGGGCTCG GCCCAGGGTC CAAGACTGGT GCTCAGTGCT
    CCGAACACTG TAACCAACCT GCATGTGGCC GCGCCGACGG GCCCCGAGGC CGCTGAGTGC
    CTAGTGTGCT CGGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG
    TGCGAGGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG
    AAGCTGCGCC CAGACGGCAC GGAGGTGGTC AGCGCAGCCC CCGCGCCCGG CCCACCGCGG
    CAGTTGGTGG AGGAGCTGCA GAGCCGCTAC AGGCAGATGG AGGAGCGCAT CACCTGTCCG
    ATTTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCACGGCGC GTGCGCGCCC
    TGTGGCGCGG CGCTCAGCGC CTGCCCGATC TGCCGCCAGC CCATCCGCGA CCGCATCCAG
    ATCTTCGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832570.1
    CDS460..3231
    Translation

    Target ORF information:

    RefSeq Version XM_024976802.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976802.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT
    ATCGATGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAATG GACCGATGAC
    ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTCGGAGA CGGGAACGTG
    GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTTAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG
    AGCGTTGTCT TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAAGCTG
    GTGGTGGAGG TGGCTCTGGG CAATGTGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA
    GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGC
    CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCGGATGAT
    GAGGGCAACA CGGCACTGCA CTACGCAGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG
    CTCTTGAGCT CAGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT
    GTGGCCGTGC AGAGGGGCTT CCTGGAAGTG GTGAAGGTCC TGTGTGAGCG TGGCTGTGAC
    GTCAACCTGC CTGATGCCCA TGCTGATACA CCCCTGCACT GCGCCATCTC AGCGGGCACT
    GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGATGT CACTGCCACC
    AACAGCCAGG GCTTCACTCT GCTGCACCAT GCGTCCCTCA AAGGCCACAC ACTAGCTGTC
    AGGAGGATTC TGGCACGGGC ACGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGTTTTACG
    GCACTGCACC TGGCCGCCTT CAACAACCAT GGGGAGGTGG CCCAGGTTCT CATCCAAGAG
    GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCACTGCA CCTGGCTGTG
    CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGATG CGGGCTGCAG CGTCAACGCC
    GAGGATGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT
    CTGGCAGCTG ATGGGAGCGC AGGGGACCCG GGTCCCCTGC AGCTGCTGTC CAGGCTACAG
    GCCTCGGGCC TTCCGAGCAG TGCAGAGCTG ACGGCAGGCG CGGCTATGGC CTACTACTTA
    GCGCTGGAGG GCGCCGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG
    GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG
    CAGGCTGGAG GCAGTGGCAG CGGGGGCTCG GCCCAGGGTC CAAGACTGGT GCTCAGTGCT
    CCGAACACTG TAACCAACCT GCATGTGGCC GCGCCGACGG GCCCCGAGGC CGCTGAGTGC
    CTAGTGTGCT CGGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG
    TGCGAGGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG
    AAGCTGCGCC CAGACGGCAC GGAGGTGGTC AGCGCAGCCC CCGCGCCCGG CCCACCGCGG
    CAGTTGGTGG AGGAGCTGCA GAGCCGCTAC AGGCAGATGG AGGAGCGCAT CACCTGTCCG
    ATTTGCATCG ACAGCCACAT CCGCCTCGTG TTCCAGTGCG GCCACGGCGC GTGCGCGCCC
    TGTGGCGCGG CGCTCAGCGC CTGCCCGATC TGCCGCCAGC CCATCCGCGA CCGCATCCAG
    ATCTTCGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223982
    Clone ID Related Accession (Same CDS sequence) XM_024976804.1
    Accession Version XM_024976804.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2511bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCGGGTCA TGCTGAGTCC CCGCCAGGGC CTCCCAAGGA TCCCGTTAAG GGGCATCTTC 
    CAGGGGGCGA AGGTGGTTCG GGGCCCCGAC TGGGAGTGGG GCTCACAGGA TGGAGGAGAA
    GGGAAGCCAG GCCGAGTAGT GGACATCCGT GGCTGGGACG TGGAGACAGG CCGGAGCGTG
    GCCAGTGTGA CGTGGGCTGA TGGCACCACC AACGTGTACC GTGTGGGCCA CAAGGGCAAG
    GTGGATCTCA AGTGTGTGGG CGAGGCAGCT GGCGGCTTCT ACTACAGGGA CCACCTCCCA
    AGGCTTGGCA AGCCCGCAGA GCTGCAGCGC AGGGTCAGTG CTGATGGCCA GCCCTTCCAG
    CGTGGGGACA AGGTCAAGTG TCTGCTGGAC GCGGATGTGC TGAGGGAGAT GCAGGAAGGC
    CACGGCGGGT GGAACCCCCG GATGGCCAAG TTTATCGGAC AGACGGGCAC CGTGCACCGC
    ATCACAGACC GTGGGGACGT GCGTGTGCAG TTCAGCCACA AGACCCGCTG GACCTTCCAC
    CCTGGGGCTC TCACCAAGCA AAACTCCTGC TTGGTGGGTG AGGTGGTGCG GGTTATCGAT
    GACCTTGACA CGGTGAAGCG GCTGCAGGCT GGCCATGGGG AATGGACCGA TGACATGGCC
    CCTGCTCTGG GCCGCATTGG GAGAGTGCTG AAGGTTTTCG GAGACGGGAA CGTGGGTGTG
    CTGGTCAGTG GGAAGCTGTG GACCTTTAGC CCCTCCTGCC TGGTGGCCTA CCGGCCTGAG
    GAAGATGCCA ACCTGGACGT AGCTGGGCGA GCCCGGGAGA ACAAAAGCTC CCTGAGCGTT
    GTCTTGGACA AGCTTCGAGC CCAGAAGAGT GACCTGGAGC ACCCAGGGAA GCTGGTGGTG
    GAGGTGGCTC TGGGCAATGT GGCCCGGGCT CTGGACCTGC TGCGGCGGCA CCCAGAACAG
    GTGGACACCA AGAATCAGGG CAGGACTGCC CTGCAGATGG CTGCCTACCT AGGCCAGGTG
    GAGCTGGTGC GGCTGCTGCT GCAGGCTCAG GCGGGCGTTG ACCTGCCGGA TGATGAGGGC
    AACACGGCAC TGCACTACGC AGCCCTGGGG AACCAGCCTG AGGCTGCCCG GGTGCTCTTG
    AGCTCAGGTT GTGGGGCCAA TGCTCTGAAC AGCACTCGGA GCTCAGCACT GCATGTGGCC
    GTGCAGAGGG GCTTCCTGGA AGTGGTGAAG GTCCTGTGTG AGCGTGGCTG TGACGTCAAC
    CTGCCTGATG CCCATGCTGA TACACCCCTG CACTGCGCCA TCTCAGCGGG CACTGGTGCC
    AGCGGCATTG TGGAGGTCCT CACCGAGGTG CCAGGCATCG ATGTCACTGC CACCAACAGC
    CAGGGCTTCA CTCTGCTGCA CCATGCGTCC CTCAAAGGCC ACACACTAGC TGTCAGGAGG
    ATTCTGGCAC GGGCACGGCA GCTGGTGGAC GCCAAGAAGG AGGATGGTTT TACGGCACTG
    CACCTGGCCG CCTTCAACAA CCATGGGGAG GTGGCCCAGG TTCTCATCCA AGAGGGCCAC
    TGTGATGTGA ACGCTCGCAA CCGTAAGCTG CAGTCCCCAC TGCACCTGGC TGTGCAGCAG
    GCCCACGTGG GGCTGGTGCC ACTGCTGGTG GATGCGGGCT GCAGCGTCAA CGCCGAGGAT
    GAGGAGGGGG ACACGGCTCT GCACGTGGCC CTGCAGCGCC ACCAGCTGCT GCCTCTGGCA
    GCTGATGGGA GCGCAGGGGA CCCGGGTCCC CTGCAGCTGC TGTCCAGGCT ACAGGCCTCG
    GGCCTTCCGA GCAGTGCAGA GCTGACGGCA GGCGCGGCTA TGGCCTACTA CTTAGCGCTG
    GAGGGCGCCG ACCTGAGCTA CGCCAACCAC CGCGGCCGGA GCCCCCTGGA CCTGGCTGCC
    GAGGGTCGCC TGCTCAAAGC CCTGCAGGGC TGTGCTCAGC GCTTCCGGGA GCGGCAGGCT
    GGAGGCAGTG GCAGCGGGGG CTCGGCCCAG GGTCCAAGAC TGGTGCTCAG TGCTCCGAAC
    ACTGTAACCA ACCTGCATGT GGCCGCGCCG ACGGGCCCCG AGGCCGCTGA GTGCCTAGTG
    TGCTCGGAGC TGGCGCTGCT GGTGCTGTTC TCGCCCTGCC AGCATCGCAC AGTGTGCGAG
    GAGTGCGCGC GCAGGATGAA GAAATGCATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTG
    CGCCCAGACC TCAGCCCCGA GCCCCCGCCC CTGACCTGGA CCTCTTCCGC AGACGGCACG
    GAGGTGGTCA GCGCAGCCCC CGCGCCCGGC CCACCGCGGC AGTTGGTGGA GGAGCTGCAG
    AGCCGCTACA GGCAGATGGA GGAGCGCATC ACCTGTCCGA TTTGCATCGA CAGCCACATC
    CGCCTCGTGT TCCAGTGCGG CCACGGCGCG TGCGCGCCCT GTGGCGCGGC GCTCAGCGCC
    TGCCCGATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA TCTTCGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832572.1
    CDS676..3186
    Translation

    Target ORF information:

    RefSeq Version XM_024976804.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976804.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCGGGTCA TGCTGAGTCC CCGCCAGGGC CTCCCAAGGA TCCCGTTAAG GGGCATCTTC 
    CAGGGGGCGA AGGTGGTTCG GGGCCCCGAC TGGGAGTGGG GCTCACAGGA TGGAGGAGAA
    GGGAAGCCAG GCCGAGTAGT GGACATCCGT GGCTGGGACG TGGAGACAGG CCGGAGCGTG
    GCCAGTGTGA CGTGGGCTGA TGGCACCACC AACGTGTACC GTGTGGGCCA CAAGGGCAAG
    GTGGATCTCA AGTGTGTGGG CGAGGCAGCT GGCGGCTTCT ACTACAGGGA CCACCTCCCA
    AGGCTTGGCA AGCCCGCAGA GCTGCAGCGC AGGGTCAGTG CTGATGGCCA GCCCTTCCAG
    CGTGGGGACA AGGTCAAGTG TCTGCTGGAC GCGGATGTGC TGAGGGAGAT GCAGGAAGGC
    CACGGCGGGT GGAACCCCCG GATGGCCAAG TTTATCGGAC AGACGGGCAC CGTGCACCGC
    ATCACAGACC GTGGGGACGT GCGTGTGCAG TTCAGCCACA AGACCCGCTG GACCTTCCAC
    CCTGGGGCTC TCACCAAGCA AAACTCCTGC TTGGTGGGTG AGGTGGTGCG GGTTATCGAT
    GACCTTGACA CGGTGAAGCG GCTGCAGGCT GGCCATGGGG AATGGACCGA TGACATGGCC
    CCTGCTCTGG GCCGCATTGG GAGAGTGCTG AAGGTTTTCG GAGACGGGAA CGTGGGTGTG
    CTGGTCAGTG GGAAGCTGTG GACCTTTAGC CCCTCCTGCC TGGTGGCCTA CCGGCCTGAG
    GAAGATGCCA ACCTGGACGT AGCTGGGCGA GCCCGGGAGA ACAAAAGCTC CCTGAGCGTT
    GTCTTGGACA AGCTTCGAGC CCAGAAGAGT GACCTGGAGC ACCCAGGGAA GCTGGTGGTG
    GAGGTGGCTC TGGGCAATGT GGCCCGGGCT CTGGACCTGC TGCGGCGGCA CCCAGAACAG
    GTGGACACCA AGAATCAGGG CAGGACTGCC CTGCAGATGG CTGCCTACCT AGGCCAGGTG
    GAGCTGGTGC GGCTGCTGCT GCAGGCTCAG GCGGGCGTTG ACCTGCCGGA TGATGAGGGC
    AACACGGCAC TGCACTACGC AGCCCTGGGG AACCAGCCTG AGGCTGCCCG GGTGCTCTTG
    AGCTCAGGTT GTGGGGCCAA TGCTCTGAAC AGCACTCGGA GCTCAGCACT GCATGTGGCC
    GTGCAGAGGG GCTTCCTGGA AGTGGTGAAG GTCCTGTGTG AGCGTGGCTG TGACGTCAAC
    CTGCCTGATG CCCATGCTGA TACACCCCTG CACTGCGCCA TCTCAGCGGG CACTGGTGCC
    AGCGGCATTG TGGAGGTCCT CACCGAGGTG CCAGGCATCG ATGTCACTGC CACCAACAGC
    CAGGGCTTCA CTCTGCTGCA CCATGCGTCC CTCAAAGGCC ACACACTAGC TGTCAGGAGG
    ATTCTGGCAC GGGCACGGCA GCTGGTGGAC GCCAAGAAGG AGGATGGTTT TACGGCACTG
    CACCTGGCCG CCTTCAACAA CCATGGGGAG GTGGCCCAGG TTCTCATCCA AGAGGGCCAC
    TGTGATGTGA ACGCTCGCAA CCGTAAGCTG CAGTCCCCAC TGCACCTGGC TGTGCAGCAG
    GCCCACGTGG GGCTGGTGCC ACTGCTGGTG GATGCGGGCT GCAGCGTCAA CGCCGAGGAT
    GAGGAGGGGG ACACGGCTCT GCACGTGGCC CTGCAGCGCC ACCAGCTGCT GCCTCTGGCA
    GCTGATGGGA GCGCAGGGGA CCCGGGTCCC CTGCAGCTGC TGTCCAGGCT ACAGGCCTCG
    GGCCTTCCGA GCAGTGCAGA GCTGACGGCA GGCGCGGCTA TGGCCTACTA CTTAGCGCTG
    GAGGGCGCCG ACCTGAGCTA CGCCAACCAC CGCGGCCGGA GCCCCCTGGA CCTGGCTGCC
    GAGGGTCGCC TGCTCAAAGC CCTGCAGGGC TGTGCTCAGC GCTTCCGGGA GCGGCAGGCT
    GGAGGCAGTG GCAGCGGGGG CTCGGCCCAG GGTCCAAGAC TGGTGCTCAG TGCTCCGAAC
    ACTGTAACCA ACCTGCATGT GGCCGCGCCG ACGGGCCCCG AGGCCGCTGA GTGCCTAGTG
    TGCTCGGAGC TGGCGCTGCT GGTGCTGTTC TCGCCCTGCC AGCATCGCAC AGTGTGCGAG
    GAGTGCGCGC GCAGGATGAA GAAATGCATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTG
    CGCCCAGACC TCAGCCCCGA GCCCCCGCCC CTGACCTGGA CCTCTTCCGC AGACGGCACG
    GAGGTGGTCA GCGCAGCCCC CGCGCCCGGC CCACCGCGGC AGTTGGTGGA GGAGCTGCAG
    AGCCGCTACA GGCAGATGGA GGAGCGCATC ACCTGTCCGA TTTGCATCGA CAGCCACATC
    CGCCTCGTGT TCCAGTGCGG CCACGGCGCG TGCGCGCCCT GTGGCGCGGC GCTCAGCGCC
    TGCCCGATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA TCTTCGTGTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223983
    Clone ID Related Accession (Same CDS sequence) XM_024976805.1
    Accession Version XM_024976805.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2502bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTGAGTC CCCGCCAGGG CCTCCCAAGG ATCCCGTTAA GGGGCATCTT CCAGGGGGCG 
    AAGGTGGTTC GGGGCCCCGA CTGGGAGTGG GGCTCACAGG ATGGAGGAGA AGGGAAGCCA
    GGCCGAGTAG TGGACATCCG TGGCTGGGAC GTGGAGACAG GCCGGAGCGT GGCCAGTGTG
    ACGTGGGCTG ATGGCACCAC CAACGTGTAC CGTGTGGGCC ACAAGGGCAA GGTGGATCTC
    AAGTGTGTGG GCGAGGCAGC TGGCGGCTTC TACTACAGGG ACCACCTCCC AAGGCTTGGC
    AAGCCCGCAG AGCTGCAGCG CAGGGTCAGT GCTGATGGCC AGCCCTTCCA GCGTGGGGAC
    AAGGTCAAGT GTCTGCTGGA CGCGGATGTG CTGAGGGAGA TGCAGGAAGG CCACGGCGGG
    TGGAACCCCC GGATGGCCAA GTTTATCGGA CAGACGGGCA CCGTGCACCG CATCACAGAC
    CGTGGGGACG TGCGTGTGCA GTTCAGCCAC AAGACCCGCT GGACCTTCCA CCCTGGGGCT
    CTCACCAAGC AAAACTCCTG CTTGGTGGGT GAGGTGGTGC GGGTTATCGA TGACCTTGAC
    ACGGTGAAGC GGCTGCAGGC TGGCCATGGG GAATGGACCG ATGACATGGC CCCTGCTCTG
    GGCCGCATTG GGAGAGTGCT GAAGGTTTTC GGAGACGGGA ACGTGGGTGT GCTGGTCAGT
    GGGAAGCTGT GGACCTTTAG CCCCTCCTGC CTGGTGGCCT ACCGGCCTGA GGAAGATGCC
    AACCTGGACG TAGCTGGGCG AGCCCGGGAG AACAAAAGCT CCCTGAGCGT TGTCTTGGAC
    AAGCTTCGAG CCCAGAAGAG TGACCTGGAG CACCCAGGGA AGCTGGTGGT GGAGGTGGCT
    CTGGGCAATG TGGCCCGGGC TCTGGACCTG CTGCGGCGGC ACCCAGAACA GGTGGACACC
    AAGAATCAGG GCAGGACTGC CCTGCAGATG GCTGCCTACC TAGGCCAGGT GGAGCTGGTG
    CGGCTGCTGC TGCAGGCTCA GGCGGGCGTT GACCTGCCGG ATGATGAGGG CAACACGGCA
    CTGCACTACG CAGCCCTGGG GAACCAGCCT GAGGCTGCCC GGGTGCTCTT GAGCTCAGGT
    TGTGGGGCCA ATGCTCTGAA CAGCACTCGG AGCTCAGCAC TGCATGTGGC CGTGCAGAGG
    GGCTTCCTGG AAGTGGTGAA GGTCCTGTGT GAGCGTGGCT GTGACGTCAA CCTGCCTGAT
    GCCCATGCTG ATACACCCCT GCACTGCGCC ATCTCAGCGG GCACTGGTGC CAGCGGCATT
    GTGGAGGTCC TCACCGAGGT GCCAGGCATC GATGTCACTG CCACCAACAG CCAGGGCTTC
    ACTCTGCTGC ACCATGCGTC CCTCAAAGGC CACACACTAG CTGTCAGGAG GATTCTGGCA
    CGGGCACGGC AGCTGGTGGA CGCCAAGAAG GAGGATGGTT TTACGGCACT GCACCTGGCC
    GCCTTCAACA ACCATGGGGA GGTGGCCCAG GTTCTCATCC AAGAGGGCCA CTGTGATGTG
    AACGCTCGCA ACCGTAAGCT GCAGTCCCCA CTGCACCTGG CTGTGCAGCA GGCCCACGTG
    GGGCTGGTGC CACTGCTGGT GGATGCGGGC TGCAGCGTCA ACGCCGAGGA TGAGGAGGGG
    GACACGGCTC TGCACGTGGC CCTGCAGCGC CACCAGCTGC TGCCTCTGGC AGCTGATGGG
    AGCGCAGGGG ACCCGGGTCC CCTGCAGCTG CTGTCCAGGC TACAGGCCTC GGGCCTTCCG
    AGCAGTGCAG AGCTGACGGC AGGCGCGGCT ATGGCCTACT ACTTAGCGCT GGAGGGCGCC
    GACCTGAGCT ACGCCAACCA CCGCGGCCGG AGCCCCCTGG ACCTGGCTGC CGAGGGTCGC
    CTGCTCAAAG CCCTGCAGGG CTGTGCTCAG CGCTTCCGGG AGCGGCAGGC TGGAGGCAGT
    GGCAGCGGGG GCTCGGCCCA GGGTCCAAGA CTGGTGCTCA GTGCTCCGAA CACTGTAACC
    AACCTGCATG TGGCCGCGCC GACGGGCCCC GAGGCCGCTG AGTGCCTAGT GTGCTCGGAG
    CTGGCGCTGC TGGTGCTGTT CTCGCCCTGC CAGCATCGCA CAGTGTGCGA GGAGTGCGCG
    CGCAGGATGA AGAAATGCAT CAGGTGCCAG GTGGTCATCA GCAAGAAGCT GCGCCCAGAC
    CTCAGCCCCG AGCCCCCGCC CCTGACCTGG ACCTCTTCCG CAGACGGCAC GGAGGTGGTC
    AGCGCAGCCC CCGCGCCCGG CCCACCGCGG CAGTTGGTGG AGGAGCTGCA GAGCCGCTAC
    AGGCAGATGG AGGAGCGCAT CACCTGTCCG ATTTGCATCG ACAGCCACAT CCGCCTCGTG
    TTCCAGTGCG GCCACGGCGC GTGCGCGCCC TGTGGCGCGG CGCTCAGCGC CTGCCCGATC
    TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832573.1
    CDS255..2756
    Translation

    Target ORF information:

    RefSeq Version XM_024976805.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976805.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCTGAGTC CCCGCCAGGG CCTCCCAAGG ATCCCGTTAA GGGGCATCTT CCAGGGGGCG 
    AAGGTGGTTC GGGGCCCCGA CTGGGAGTGG GGCTCACAGG ATGGAGGAGA AGGGAAGCCA
    GGCCGAGTAG TGGACATCCG TGGCTGGGAC GTGGAGACAG GCCGGAGCGT GGCCAGTGTG
    ACGTGGGCTG ATGGCACCAC CAACGTGTAC CGTGTGGGCC ACAAGGGCAA GGTGGATCTC
    AAGTGTGTGG GCGAGGCAGC TGGCGGCTTC TACTACAGGG ACCACCTCCC AAGGCTTGGC
    AAGCCCGCAG AGCTGCAGCG CAGGGTCAGT GCTGATGGCC AGCCCTTCCA GCGTGGGGAC
    AAGGTCAAGT GTCTGCTGGA CGCGGATGTG CTGAGGGAGA TGCAGGAAGG CCACGGCGGG
    TGGAACCCCC GGATGGCCAA GTTTATCGGA CAGACGGGCA CCGTGCACCG CATCACAGAC
    CGTGGGGACG TGCGTGTGCA GTTCAGCCAC AAGACCCGCT GGACCTTCCA CCCTGGGGCT
    CTCACCAAGC AAAACTCCTG CTTGGTGGGT GAGGTGGTGC GGGTTATCGA TGACCTTGAC
    ACGGTGAAGC GGCTGCAGGC TGGCCATGGG GAATGGACCG ATGACATGGC CCCTGCTCTG
    GGCCGCATTG GGAGAGTGCT GAAGGTTTTC GGAGACGGGA ACGTGGGTGT GCTGGTCAGT
    GGGAAGCTGT GGACCTTTAG CCCCTCCTGC CTGGTGGCCT ACCGGCCTGA GGAAGATGCC
    AACCTGGACG TAGCTGGGCG AGCCCGGGAG AACAAAAGCT CCCTGAGCGT TGTCTTGGAC
    AAGCTTCGAG CCCAGAAGAG TGACCTGGAG CACCCAGGGA AGCTGGTGGT GGAGGTGGCT
    CTGGGCAATG TGGCCCGGGC TCTGGACCTG CTGCGGCGGC ACCCAGAACA GGTGGACACC
    AAGAATCAGG GCAGGACTGC CCTGCAGATG GCTGCCTACC TAGGCCAGGT GGAGCTGGTG
    CGGCTGCTGC TGCAGGCTCA GGCGGGCGTT GACCTGCCGG ATGATGAGGG CAACACGGCA
    CTGCACTACG CAGCCCTGGG GAACCAGCCT GAGGCTGCCC GGGTGCTCTT GAGCTCAGGT
    TGTGGGGCCA ATGCTCTGAA CAGCACTCGG AGCTCAGCAC TGCATGTGGC CGTGCAGAGG
    GGCTTCCTGG AAGTGGTGAA GGTCCTGTGT GAGCGTGGCT GTGACGTCAA CCTGCCTGAT
    GCCCATGCTG ATACACCCCT GCACTGCGCC ATCTCAGCGG GCACTGGTGC CAGCGGCATT
    GTGGAGGTCC TCACCGAGGT GCCAGGCATC GATGTCACTG CCACCAACAG CCAGGGCTTC
    ACTCTGCTGC ACCATGCGTC CCTCAAAGGC CACACACTAG CTGTCAGGAG GATTCTGGCA
    CGGGCACGGC AGCTGGTGGA CGCCAAGAAG GAGGATGGTT TTACGGCACT GCACCTGGCC
    GCCTTCAACA ACCATGGGGA GGTGGCCCAG GTTCTCATCC AAGAGGGCCA CTGTGATGTG
    AACGCTCGCA ACCGTAAGCT GCAGTCCCCA CTGCACCTGG CTGTGCAGCA GGCCCACGTG
    GGGCTGGTGC CACTGCTGGT GGATGCGGGC TGCAGCGTCA ACGCCGAGGA TGAGGAGGGG
    GACACGGCTC TGCACGTGGC CCTGCAGCGC CACCAGCTGC TGCCTCTGGC AGCTGATGGG
    AGCGCAGGGG ACCCGGGTCC CCTGCAGCTG CTGTCCAGGC TACAGGCCTC GGGCCTTCCG
    AGCAGTGCAG AGCTGACGGC AGGCGCGGCT ATGGCCTACT ACTTAGCGCT GGAGGGCGCC
    GACCTGAGCT ACGCCAACCA CCGCGGCCGG AGCCCCCTGG ACCTGGCTGC CGAGGGTCGC
    CTGCTCAAAG CCCTGCAGGG CTGTGCTCAG CGCTTCCGGG AGCGGCAGGC TGGAGGCAGT
    GGCAGCGGGG GCTCGGCCCA GGGTCCAAGA CTGGTGCTCA GTGCTCCGAA CACTGTAACC
    AACCTGCATG TGGCCGCGCC GACGGGCCCC GAGGCCGCTG AGTGCCTAGT GTGCTCGGAG
    CTGGCGCTGC TGGTGCTGTT CTCGCCCTGC CAGCATCGCA CAGTGTGCGA GGAGTGCGCG
    CGCAGGATGA AGAAATGCAT CAGGTGCCAG GTGGTCATCA GCAAGAAGCT GCGCCCAGAC
    CTCAGCCCCG AGCCCCCGCC CCTGACCTGG ACCTCTTCCG CAGACGGCAC GGAGGTGGTC
    AGCGCAGCCC CCGCGCCCGG CCCACCGCGG CAGTTGGTGG AGGAGCTGCA GAGCCGCTAC
    AGGCAGATGG AGGAGCGCAT CACCTGTCCG ATTTGCATCG ACAGCCACAT CCGCCTCGTG
    TTCCAGTGCG GCCACGGCGC GTGCGCGCCC TGTGGCGCGG CGCTCAGCGC CTGCCCGATC
    TGCCGCCAGC CCATCCGCGA CCGCATCCAG ATCTTCGTGT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa223984
    Clone ID Related Accession (Same CDS sequence) XM_024976806.1
    Accession Version XM_024976806.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2466bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCGGGTCA TGCTGAGTCC CCGCCAGGGC CTCCCAAGGA TCCCGTTAAG GGGCATCTTC 
    CAGGGGGCGA AGGTGGTTCG GGGCCCCGAC TGGGAGTGGG GCTCACAGGA TGGAGGAGAA
    GGGAAGCCAG GCCGAGTAGT GGACATCCGT GGCTGGGACG TGGAGACAGG CCGGAGCGTG
    GCCAGTGTGA CGTGGGCTGA TGGCACCACC AACGTGTACC GTGTGGGCCA CAAGGGCAAG
    GTGGATCTCA AGTGTGTGGG CGAGGCAGCT GGCGGCTTCT ACTACAGGGA CCACCTCCCA
    AGGCTTGGCA AGCCCGCAGA GCTGCAGCGC AGGGTCAGTG CTGATGGCCA GCCCTTCCAG
    CGTGGGGACA AGGTCAAGTG TCTGCTGGAC GCGGATGTGC TGAGGGAGAT GCAGGAAGGC
    CACGGCGGGT GGAACCCCCG GATGGCCAAG TTTATCGGAC AGACGGGCAC CGTGCACCGC
    ATCACAGACC GTGGGGACGT GCGTGTGCAG TTCAGCCACA AGACCCGCTG GACCTTCCAC
    CCTGGGGCTC TCACCAAGCA AAACTCCTGC TTGGTGGGTG AGGTGGTGCG GGTTATCGAT
    GACCTTGACA CGGTGAAGCG GCTGCAGGCT GGCCATGGGG AATGGACCGA TGACATGGCC
    CCTGCTCTGG GCCGCATTGG GAGAGTGCTG AAGGTTTTCG GAGACGGGAA CGTGGGTGTG
    CTGGTCAGTG GGAAGCTGTG GACCTTTAGC CCCTCCTGCC TGGTGGCCTA CCGGCCTGAG
    GAAGATGCCA ACCTGGACGT AGCTGGGCGA GCCCGGGAGA ACAAAAGCTC CCTGAGCGTT
    GTCTTGGACA AGCTTCGAGC CCAGAAGAGT GACCTGGAGC ACCCAGGGAA GCTGGTGGTG
    GAGGTGGCTC TGGGCAATGT GGCCCGGGCT CTGGACCTGC TGCGGCGGCA CCCAGAACAG
    GTGGACACCA AGAATCAGGG CAGGACTGCC CTGCAGATGG CTGCCTACCT AGGCCAGGTG
    GAGCTGGTGC GGCTGCTGCT GCAGGCTCAG GCGGGCGTTG ACCTGCCGGA TGATGAGGGC
    AACACGGCAC TGCACTACGC AGCCCTGGGG AACCAGCCTG AGGCTGCCCG GGTGCTCTTG
    AGCTCAGGTT GTGGGGCCAA TGCTCTGAAC AGCACTCGGA GCTCAGCACT GCATGTGGCC
    GTGCAGAGGG GCTTCCTGGA AGTGGTGAAG GTCCTGTGTG AGCGTGGCTG TGACGTCAAC
    CTGCCTGATG CCCATGCTGA TACACCCCTG CACTGCGCCA TCTCAGCGGG CACTGGTGCC
    AGCGGCATTG TGGAGGTCCT CACCGAGGTG CCAGGCATCG ATGTCACTGC CACCAACAGC
    CAGGGCTTCA CTCTGCTGCA CCATGCGTCC CTCAAAGGCC ACACACTAGC TGTCAGGAGG
    ATTCTGGCAC GGGCACGGCA GCTGGTGGAC GCCAAGAAGG AGGATGGTTT TACGGCACTG
    CACCTGGCCG CCTTCAACAA CCATGGGGAG GTGGCCCAGG TTCTCATCCA AGAGGGCCAC
    TGTGATGTGA ACGCTCGCAA CCGTAAGCTG CAGTCCCCAC TGCACCTGGC TGTGCAGCAG
    GCCCACGTGG GGCTGGTGCC ACTGCTGGTG GATGCGGGCT GCAGCGTCAA CGCCGAGGAT
    GAGGAGGGGG ACACGGCTCT GCACGTGGCC CTGCAGCGCC ACCAGCTGCT GCCTCTGGCA
    GCTGATGGGA GCGCAGGGGA CCCGGGTCCC CTGCAGCTGC TGTCCAGGCT ACAGGCCTCG
    GGCCTTCCGA GCAGTGCAGA GCTGACGGCA GGCGCGGCTA TGGCCTACTA CTTAGCGCTG
    GAGGGCGCCG ACCTGAGCTA CGCCAACCAC CGCGGCCGGA GCCCCCTGGA CCTGGCTGCC
    GAGGGTCGCC TGCTCAAAGC CCTGCAGGGC TGTGCTCAGC GCTTCCGGGA GCGGCAGGCT
    GGAGGCAGTG GCAGCGGGGG CTCGGCCCAG GGTCCAAGAC TGGTGCTCAG TGCTCCGAAC
    ACTGTAACCA ACCTGCATGT GGCCGCGCCG ACGGGCCCCG AGGCCGCTGA GTGCCTAGTG
    TGCTCGGAGC TGGCGCTGCT GGTGCTGTTC TCGCCCTGCC AGCATCGCAC AGTGTGCGAG
    GAGTGCGCGC GCAGGATGAA GAAATGCATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTG
    CGCCCAGACG GCACGGAGGT GGTCAGCGCA GCCCCCGCGC CCGGCCCACC GCGGCAGTTG
    GTGGAGGAGC TGCAGAGCCG CTACAGGCAG ATGGAGGAGC GCATCACCTG TCCGATTTGC
    ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCACG GCGCGTGCGC GCCCTGTGGC
    GCGGCGCTCA GCGCCTGCCC GATCTGCCGC CAGCCCATCC GCGACCGCAT CCAGATCTTC
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024832574.1
    CDS3272..5737
    Translation

    Target ORF information:

    RefSeq Version XM_024976806.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024976806.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    ATGCGGGTCA TGCTGAGTCC CCGCCAGGGC CTCCCAAGGA TCCCGTTAAG GGGCATCTTC 
    CAGGGGGCGA AGGTGGTTCG GGGCCCCGAC TGGGAGTGGG GCTCACAGGA TGGAGGAGAA
    GGGAAGCCAG GCCGAGTAGT GGACATCCGT GGCTGGGACG TGGAGACAGG CCGGAGCGTG
    GCCAGTGTGA CGTGGGCTGA TGGCACCACC AACGTGTACC GTGTGGGCCA CAAGGGCAAG
    GTGGATCTCA AGTGTGTGGG CGAGGCAGCT GGCGGCTTCT ACTACAGGGA CCACCTCCCA
    AGGCTTGGCA AGCCCGCAGA GCTGCAGCGC AGGGTCAGTG CTGATGGCCA GCCCTTCCAG
    CGTGGGGACA AGGTCAAGTG TCTGCTGGAC GCGGATGTGC TGAGGGAGAT GCAGGAAGGC
    CACGGCGGGT GGAACCCCCG GATGGCCAAG TTTATCGGAC AGACGGGCAC CGTGCACCGC
    ATCACAGACC GTGGGGACGT GCGTGTGCAG TTCAGCCACA AGACCCGCTG GACCTTCCAC
    CCTGGGGCTC TCACCAAGCA AAACTCCTGC TTGGTGGGTG AGGTGGTGCG GGTTATCGAT
    GACCTTGACA CGGTGAAGCG GCTGCAGGCT GGCCATGGGG AATGGACCGA TGACATGGCC
    CCTGCTCTGG GCCGCATTGG GAGAGTGCTG AAGGTTTTCG GAGACGGGAA CGTGGGTGTG
    CTGGTCAGTG GGAAGCTGTG GACCTTTAGC CCCTCCTGCC TGGTGGCCTA CCGGCCTGAG
    GAAGATGCCA ACCTGGACGT AGCTGGGCGA GCCCGGGAGA ACAAAAGCTC CCTGAGCGTT
    GTCTTGGACA AGCTTCGAGC CCAGAAGAGT GACCTGGAGC ACCCAGGGAA GCTGGTGGTG
    GAGGTGGCTC TGGGCAATGT GGCCCGGGCT CTGGACCTGC TGCGGCGGCA CCCAGAACAG
    GTGGACACCA AGAATCAGGG CAGGACTGCC CTGCAGATGG CTGCCTACCT AGGCCAGGTG
    GAGCTGGTGC GGCTGCTGCT GCAGGCTCAG GCGGGCGTTG ACCTGCCGGA TGATGAGGGC
    AACACGGCAC TGCACTACGC AGCCCTGGGG AACCAGCCTG AGGCTGCCCG GGTGCTCTTG
    AGCTCAGGTT GTGGGGCCAA TGCTCTGAAC AGCACTCGGA GCTCAGCACT GCATGTGGCC
    GTGCAGAGGG GCTTCCTGGA AGTGGTGAAG GTCCTGTGTG AGCGTGGCTG TGACGTCAAC
    CTGCCTGATG CCCATGCTGA TACACCCCTG CACTGCGCCA TCTCAGCGGG CACTGGTGCC
    AGCGGCATTG TGGAGGTCCT CACCGAGGTG CCAGGCATCG ATGTCACTGC CACCAACAGC
    CAGGGCTTCA CTCTGCTGCA CCATGCGTCC CTCAAAGGCC ACACACTAGC TGTCAGGAGG
    ATTCTGGCAC GGGCACGGCA GCTGGTGGAC GCCAAGAAGG AGGATGGTTT TACGGCACTG
    CACCTGGCCG CCTTCAACAA CCATGGGGAG GTGGCCCAGG TTCTCATCCA AGAGGGCCAC
    TGTGATGTGA ACGCTCGCAA CCGTAAGCTG CAGTCCCCAC TGCACCTGGC TGTGCAGCAG
    GCCCACGTGG GGCTGGTGCC ACTGCTGGTG GATGCGGGCT GCAGCGTCAA CGCCGAGGAT
    GAGGAGGGGG ACACGGCTCT GCACGTGGCC CTGCAGCGCC ACCAGCTGCT GCCTCTGGCA
    GCTGATGGGA GCGCAGGGGA CCCGGGTCCC CTGCAGCTGC TGTCCAGGCT ACAGGCCTCG
    GGCCTTCCGA GCAGTGCAGA GCTGACGGCA GGCGCGGCTA TGGCCTACTA CTTAGCGCTG
    GAGGGCGCCG ACCTGAGCTA CGCCAACCAC CGCGGCCGGA GCCCCCTGGA CCTGGCTGCC
    GAGGGTCGCC TGCTCAAAGC CCTGCAGGGC TGTGCTCAGC GCTTCCGGGA GCGGCAGGCT
    GGAGGCAGTG GCAGCGGGGG CTCGGCCCAG GGTCCAAGAC TGGTGCTCAG TGCTCCGAAC
    ACTGTAACCA ACCTGCATGT GGCCGCGCCG ACGGGCCCCG AGGCCGCTGA GTGCCTAGTG
    TGCTCGGAGC TGGCGCTGCT GGTGCTGTTC TCGCCCTGCC AGCATCGCAC AGTGTGCGAG
    GAGTGCGCGC GCAGGATGAA GAAATGCATC AGGTGCCAGG TGGTCATCAG CAAGAAGCTG
    CGCCCAGACG GCACGGAGGT GGTCAGCGCA GCCCCCGCGC CCGGCCCACC GCGGCAGTTG
    GTGGAGGAGC TGCAGAGCCG CTACAGGCAG ATGGAGGAGC GCATCACCTG TCCGATTTGC
    ATCGACAGCC ACATCCGCCT CGTGTTCCAG TGCGGCCACG GCGCGTGCGC GCCCTGTGGC
    GCGGCGCTCA GCGCCTGCCC GATCTGCCGC CAGCCCATCC GCGACCGCAT CCAGATCTTC
    GTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94880
    Clone ID Related Accession (Same CDS sequence) XM_015466875.1
    Accession Version XM_015466875.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2952bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC
    GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTCGCCCAG
    ACGGCACGGA GGTGGTCAGC GCAGCCCCCG CGCCCGGCCC ACCGCGGCAG TTGGTGGAGG
    AGCTGCAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGTCCGATT TGCATCGACA
    GCCACATCCG CCTCGTGTTC CAGTGCGGCC ACGGCGCGTG CGCGCCCTGT GGCGCGGCGC
    TCAGCGCCTG CCCGATCTGC CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGTAGC
    CGGCGCCCCC CGCCTCGTTC CGGGGCCATG CCCCGCGGCC GCCCTCACGA GCCCTTTCTC
    TGTATTTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322361.1
    CDS1594..4545
    Translation

    Target ORF information:

    RefSeq Version XM_015466875.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466875.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC
    GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTCGCCCAG
    ACGGCACGGA GGTGGTCAGC GCAGCCCCCG CGCCCGGCCC ACCGCGGCAG TTGGTGGAGG
    AGCTGCAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGTCCGATT TGCATCGACA
    GCCACATCCG CCTCGTGTTC CAGTGCGGCC ACGGCGCGTG CGCGCCCTGT GGCGCGGCGC
    TCAGCGCCTG CCCGATCTGC CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGTAGC
    CGGCGCCCCC CGCCTCGTTC CGGGGCCATG CCCCGCGGCC GCCCTCACGA GCCCTTTCTC
    TGTATTTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94881
    Clone ID Related Accession (Same CDS sequence) XM_015466876.1
    Accession Version XM_015466876.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2940bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC
    GTGCGTGTGC AGTTCAGCCA CAAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG
    CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ATGACCTTGA CACGGTGAAG
    CGGCTGCAGG CTGGCCATGG GGAATGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT
    GGGAGAGTGC TGAAGGTTTT CGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG
    TGGACCTTTA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC
    GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGCG TTGTCTTGGA CAAGCTTCGA
    GCCCAGAAGA GTGACCTGGA GCACCCAGGG AAGCTGGTGG TGGAGGTGGC TCTGGGCAAT
    GTGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG
    GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGCCAGG TGGAGCTGGT GCGGCTGCTG
    CTGCAGGCTC AGGCGGGCGT TGACCTGCCG GATGATGAGG GCAACACGGC ACTGCACTAC
    GCAGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCAGG TTGTGGGGCC
    AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CCGTGCAGAG GGGCTTCCTG
    GAAGTGGTGA AGGTCCTGTG TGAGCGTGGC TGTGACGTCA ACCTGCCTGA TGCCCATGCT
    GATACACCCC TGCACTGCGC CATCTCAGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC
    CTCACCGAGG TGCCAGGCAT CGATGTCACT GCCACCAACA GCCAGGGCTT CACTCTGCTG
    CACCATGCGT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC ACGGGCACGG
    CAGCTGGTGG ACGCCAAGAA GGAGGATGGT TTTACGGCAC TGCACCTGGC CGCCTTCAAC
    AACCATGGGG AGGTGGCCCA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC
    AACCGTAAGC TGCAGTCCCC ACTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG
    CCACTGCTGG TGGATGCGGG CTGCAGCGTC AACGCCGAGG ATGAGGAGGG GGACACGGCT
    CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG CAGCTGATGG GAGCGCAGGG
    GACCCGGGTC CCCTGCAGCT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC GAGCAGTGCA
    GAGCTGACGG CAGGCGCGGC TATGGCCTAC TACTTAGCGC TGGAGGGCGC CGACCTGAGC
    TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA
    GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTGGAGGCAG TGGCAGCGGG
    GGCTCGGCCC AGGGTCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTAAC CAACCTGCAT
    GTGGCCGCGC CGACGGGCCC CGAGGCCGCT GAGTGCCTAG TGTGCTCGGA GCTGGCGCTG
    CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGAGTGCGC GCGCAGGATG
    AAGAAATGCA TCAGGTGCCA GGTGGTCATC AGCAAGAAGC TCGCCCAGAC GGCACGGAGG
    TGGTCAGCGC AGCCCCCGCG CCCGGCCCAC CGCGGCAGTT GGTGGAGGAG CTGCAGAGCC
    GCTACAGGCA GATGGAGGAG CGCATCACCT GTCCGATTTG CATCGACAGC CACATCCGCC
    TCGTGTTCCA GTGCGGCCAC GGCGCGTGCG CGCCCTGTGG CGCGGCGCTC AGCGCCTGCC
    CGATCTGCCG CCAGCCCATC CGCGACCGCA TCCAGATCTT CGTGTAGCCG GCGCCCCCCG
    CCTCGTTCCG GGGCCATGCC CCGCGGCCGC CCTCACGAGC CCTTTCTCTG TATTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322362.1
    CDS1594..4533
    Translation

    Target ORF information:

    RefSeq Version XM_015466876.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466876.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC
    GTGCGTGTGC AGTTCAGCCA CAAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG
    CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ATGACCTTGA CACGGTGAAG
    CGGCTGCAGG CTGGCCATGG GGAATGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT
    GGGAGAGTGC TGAAGGTTTT CGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG
    TGGACCTTTA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC
    GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGCG TTGTCTTGGA CAAGCTTCGA
    GCCCAGAAGA GTGACCTGGA GCACCCAGGG AAGCTGGTGG TGGAGGTGGC TCTGGGCAAT
    GTGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG
    GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGCCAGG TGGAGCTGGT GCGGCTGCTG
    CTGCAGGCTC AGGCGGGCGT TGACCTGCCG GATGATGAGG GCAACACGGC ACTGCACTAC
    GCAGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCAGG TTGTGGGGCC
    AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CCGTGCAGAG GGGCTTCCTG
    GAAGTGGTGA AGGTCCTGTG TGAGCGTGGC TGTGACGTCA ACCTGCCTGA TGCCCATGCT
    GATACACCCC TGCACTGCGC CATCTCAGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC
    CTCACCGAGG TGCCAGGCAT CGATGTCACT GCCACCAACA GCCAGGGCTT CACTCTGCTG
    CACCATGCGT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC ACGGGCACGG
    CAGCTGGTGG ACGCCAAGAA GGAGGATGGT TTTACGGCAC TGCACCTGGC CGCCTTCAAC
    AACCATGGGG AGGTGGCCCA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC
    AACCGTAAGC TGCAGTCCCC ACTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG
    CCACTGCTGG TGGATGCGGG CTGCAGCGTC AACGCCGAGG ATGAGGAGGG GGACACGGCT
    CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG CAGCTGATGG GAGCGCAGGG
    GACCCGGGTC CCCTGCAGCT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC GAGCAGTGCA
    GAGCTGACGG CAGGCGCGGC TATGGCCTAC TACTTAGCGC TGGAGGGCGC CGACCTGAGC
    TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA
    GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTGGAGGCAG TGGCAGCGGG
    GGCTCGGCCC AGGGTCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTAAC CAACCTGCAT
    GTGGCCGCGC CGACGGGCCC CGAGGCCGCT GAGTGCCTAG TGTGCTCGGA GCTGGCGCTG
    CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGAGTGCGC GCGCAGGATG
    AAGAAATGCA TCAGGTGCCA GGTGGTCATC AGCAAGAAGC TCGCCCAGAC GGCACGGAGG
    TGGTCAGCGC AGCCCCCGCG CCCGGCCCAC CGCGGCAGTT GGTGGAGGAG CTGCAGAGCC
    GCTACAGGCA GATGGAGGAG CGCATCACCT GTCCGATTTG CATCGACAGC CACATCCGCC
    TCGTGTTCCA GTGCGGCCAC GGCGCGTGCG CGCCCTGTGG CGCGGCGCTC AGCGCCTGCC
    CGATCTGCCG CCAGCCCATC CGCGACCGCA TCCAGATCTT CGTGTAGCCG GCGCCCCCCG
    CCTCGTTCCG GGGCCATGCC CCGCGGCCGC CCTCACGAGC CCTTTCTCTG TATTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94882
    Clone ID Related Accession (Same CDS sequence) XM_015466877.1
    Accession Version XM_015466877.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2916bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT
    ATCGATGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAATG GACCGATGAC
    ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTCGGAGA CGGGAACGTG
    GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTTAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG
    AGCGTTGTCT TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAAGCTG
    GTGGTGGAGG TGGCTCTGGG CAATGTGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA
    GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGC
    CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCGGATGAT
    GAGGGCAACA CGGCACTGCA CTACGCAGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG
    CTCTTGAGCT CAGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT
    GTGGCCGTGC AGAGGGGCTT CCTGGAAGTG GTGAAGGTCC TGTGTGAGCG TGGCTGTGAC
    GTCAACCTGC CTGATGCCCA TGCTGATACA CCCCTGCACT GCGCCATCTC AGCGGGCACT
    GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGATGT CACTGCCACC
    AACAGCCAGG GCTTCACTCT GCTGCACCAT GCGTCCCTCA AAGGCCACAC ACTAGCTGTC
    AGGAGGATTC TGGCACGGGC ACGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGTTTTACG
    GCACTGCACC TGGCCGCCTT CAACAACCAT GGGGAGGTGG CCCAGGTTCT CATCCAAGAG
    GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCACTGCA CCTGGCTGTG
    CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGATG CGGGCTGCAG CGTCAACGCC
    GAGGATGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT
    CTGGCAGCTG ATGGGAGCGC AGGGGACCCG GGTCCCCTGC AGCTGCTGTC CAGGCTACAG
    GCCTCGGGCC TTCCGAGCAG TGCAGAGCTG ACGGCAGGCG CGGCTATGGC CTACTACTTA
    GCGCTGGAGG GCGCCGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG
    GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG
    CAGGCTGGAG GCAGTGGCAG CGGGGGCTCG GCCCAGGGTC CAAGACTGGT GCTCAGTGCT
    CCGAACACTG TAACCAACCT GCATGTGGCC GCGCCGACGG GCCCCGAGGC CGCTGAGTGC
    CTAGTGTGCT CGGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG
    TGCGAGGGTG AGTGGCCGGG TGGGGAGGGG GCCGCGGCCG GCTGGACGGC CCTCACTCAA
    CCGTGCCCTC CCCCCGCAGA GTGCGCGCGC AGGATGAAGA AATGCATCAG GTGCCAGGTG
    GTCATCAGCA AGAAGCTCGC CCAGACGGCA CGGAGGTGGT CAGCGCAGCC CCCGCGCCCG
    GCCCACCGCG GCAGTTGGTG GAGGAGCTGC AGAGCCGCTA CAGGCAGATG GAGGAGCGCA
    TCACCTGTCC GATTTGCATC GACAGCCACA TCCGCCTCGT GTTCCAGTGC GGCCACGGCG
    CGTGCGCGCC CTGTGGCGCG GCGCTCAGCG CCTGCCCGAT CTGCCGCCAG CCCATCCGCG
    ACCGCATCCA GATCTTCGTG TAGCCGGCGC CCCCCGCCTC GTTCCGGGGC CATGCCCCGC
    GGCCGCCCTC ACGAGCCCTT TCTCTGTATT TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322363.1
    CDS1594..4509
    Translation

    Target ORF information:

    RefSeq Version XM_015466877.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466877.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT
    ATCGATGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAATG GACCGATGAC
    ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTCGGAGA CGGGAACGTG
    GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTTAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG
    AGCGTTGTCT TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAAGCTG
    GTGGTGGAGG TGGCTCTGGG CAATGTGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA
    GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGC
    CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCGGATGAT
    GAGGGCAACA CGGCACTGCA CTACGCAGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG
    CTCTTGAGCT CAGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT
    GTGGCCGTGC AGAGGGGCTT CCTGGAAGTG GTGAAGGTCC TGTGTGAGCG TGGCTGTGAC
    GTCAACCTGC CTGATGCCCA TGCTGATACA CCCCTGCACT GCGCCATCTC AGCGGGCACT
    GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGATGT CACTGCCACC
    AACAGCCAGG GCTTCACTCT GCTGCACCAT GCGTCCCTCA AAGGCCACAC ACTAGCTGTC
    AGGAGGATTC TGGCACGGGC ACGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGTTTTACG
    GCACTGCACC TGGCCGCCTT CAACAACCAT GGGGAGGTGG CCCAGGTTCT CATCCAAGAG
    GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCACTGCA CCTGGCTGTG
    CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGATG CGGGCTGCAG CGTCAACGCC
    GAGGATGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT
    CTGGCAGCTG ATGGGAGCGC AGGGGACCCG GGTCCCCTGC AGCTGCTGTC CAGGCTACAG
    GCCTCGGGCC TTCCGAGCAG TGCAGAGCTG ACGGCAGGCG CGGCTATGGC CTACTACTTA
    GCGCTGGAGG GCGCCGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG
    GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG
    CAGGCTGGAG GCAGTGGCAG CGGGGGCTCG GCCCAGGGTC CAAGACTGGT GCTCAGTGCT
    CCGAACACTG TAACCAACCT GCATGTGGCC GCGCCGACGG GCCCCGAGGC CGCTGAGTGC
    CTAGTGTGCT CGGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG
    TGCGAGGGTG AGTGGCCGGG TGGGGAGGGG GCCGCGGCCG GCTGGACGGC CCTCACTCAA
    CCGTGCCCTC CCCCCGCAGA GTGCGCGCGC AGGATGAAGA AATGCATCAG GTGCCAGGTG
    GTCATCAGCA AGAAGCTCGC CCAGACGGCA CGGAGGTGGT CAGCGCAGCC CCCGCGCCCG
    GCCCACCGCG GCAGTTGGTG GAGGAGCTGC AGAGCCGCTA CAGGCAGATG GAGGAGCGCA
    TCACCTGTCC GATTTGCATC GACAGCCACA TCCGCCTCGT GTTCCAGTGC GGCCACGGCG
    CGTGCGCGCC CTGTGGCGCG GCGCTCAGCG CCTGCCCGAT CTGCCGCCAG CCCATCCGCG
    ACCGCATCCA GATCTTCGTG TAGCCGGCGC CCCCCGCCTC GTTCCGGGGC CATGCCCCGC
    GGCCGCCCTC ACGAGCCCTT TCTCTGTATT TTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94883
    Clone ID Related Accession (Same CDS sequence) XM_015466878.1
    Accession Version XM_015466878.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2844bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT
    ATCGATGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAATG GACCGATGAC
    ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTCGGAGA CGGGAACGTG
    GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTTAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG
    AGCGTTGTCT TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAAGCTG
    GTGGTGGAGG TGGCTCTGGG CAATGTGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA
    GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGC
    CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCGGATGAT
    GAGGGCAACA CGGCACTGCA CTACGCAGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG
    CTCTTGAGCT CAGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT
    GTGGCCGTGC AGAGGGGCTT CCTGGAAGTG GTGAAGGTCC TGTGTGAGCG TGGCTGTGAC
    GTCAACCTGC CTGATGCCCA TGCTGATACA CCCCTGCACT GCGCCATCTC AGCGGGCACT
    GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGATGT CACTGCCACC
    AACAGCCAGG GCTTCACTCT GCTGCACCAT GCGTCCCTCA AAGGCCACAC ACTAGCTGTC
    AGGAGGATTC TGGCACGGGC ACGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGTTTTACG
    GCACTGCACC TGGCCGCCTT CAACAACCAT GGGGAGGTGG CCCAGGTTCT CATCCAAGAG
    GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCACTGCA CCTGGCTGTG
    CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGATG CGGGCTGCAG CGTCAACGCC
    GAGGATGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT
    CTGGCAGCTG ATGGGAGCGC AGGGGACCCG GGTCCCCTGC AGCTGCTGTC CAGGCTACAG
    GCCTCGGGCC TTCCGAGCAG TGCAGAGCTG ACGGCAGGCG CGGCTATGGC CTACTACTTA
    GCGCTGGAGG GCGCCGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG
    GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG
    CAGGCTGGAG GCAGTGGCAG CGGGGGCTCG GCCCAGGGTC CAAGACTGGT GCTCAGTGCT
    CCGAACACTG TAACCAACCT GCATGTGGCC GCGCCGACGG GCCCCGAGGC CGCTGAGTGC
    CTAGTGTGCT CGGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG
    TGCGAGGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG
    AAGCTCGCCC AGACGGCACG GAGGTGGTCA GCGCAGCCCC CGCGCCCGGC CCACCGCGGC
    AGTTGGTGGA GGAGCTGCAG AGCCGCTACA GGCAGATGGA GGAGCGCATC ACCTGTCCGA
    TTTGCATCGA CAGCCACATC CGCCTCGTGT TCCAGTGCGG CCACGGCGCG TGCGCGCCCT
    GTGGCGCGGC GCTCAGCGCC TGCCCGATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA
    TCTTCGTGTA GCCGGCGCCC CCCGCCTCGT TCCGGGGCCA TGCCCCGCGG CCGCCCTCAC
    GAGCCCTTTC TCTGTATTTT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322364.1
    CDS1594..4437
    Translation

    Target ORF information:

    RefSeq Version XM_015466878.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466878.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGCAAAAC TCCTGCTTGG TGGGTGAGGT GGTGCGGGTT
    ATCGATGACC TTGACACGGT GAAGCGGCTG CAGGCTGGCC ATGGGGAATG GACCGATGAC
    ATGGCCCCTG CTCTGGGCCG CATTGGGAGA GTGCTGAAGG TTTTCGGAGA CGGGAACGTG
    GGTGTGCTGG TCAGTGGGAA GCTGTGGACC TTTAGCCCCT CCTGCCTGGT GGCCTACCGG
    CCTGAGGAAG ATGCCAACCT GGACGTAGCT GGGCGAGCCC GGGAGAACAA AAGCTCCCTG
    AGCGTTGTCT TGGACAAGCT TCGAGCCCAG AAGAGTGACC TGGAGCACCC AGGGAAGCTG
    GTGGTGGAGG TGGCTCTGGG CAATGTGGCC CGGGCTCTGG ACCTGCTGCG GCGGCACCCA
    GAACAGGTGG ACACCAAGAA TCAGGGCAGG ACTGCCCTGC AGATGGCTGC CTACCTAGGC
    CAGGTGGAGC TGGTGCGGCT GCTGCTGCAG GCTCAGGCGG GCGTTGACCT GCCGGATGAT
    GAGGGCAACA CGGCACTGCA CTACGCAGCC CTGGGGAACC AGCCTGAGGC TGCCCGGGTG
    CTCTTGAGCT CAGGTTGTGG GGCCAATGCT CTGAACAGCA CTCGGAGCTC AGCACTGCAT
    GTGGCCGTGC AGAGGGGCTT CCTGGAAGTG GTGAAGGTCC TGTGTGAGCG TGGCTGTGAC
    GTCAACCTGC CTGATGCCCA TGCTGATACA CCCCTGCACT GCGCCATCTC AGCGGGCACT
    GGTGCCAGCG GCATTGTGGA GGTCCTCACC GAGGTGCCAG GCATCGATGT CACTGCCACC
    AACAGCCAGG GCTTCACTCT GCTGCACCAT GCGTCCCTCA AAGGCCACAC ACTAGCTGTC
    AGGAGGATTC TGGCACGGGC ACGGCAGCTG GTGGACGCCA AGAAGGAGGA TGGTTTTACG
    GCACTGCACC TGGCCGCCTT CAACAACCAT GGGGAGGTGG CCCAGGTTCT CATCCAAGAG
    GGCCACTGTG ATGTGAACGC TCGCAACCGT AAGCTGCAGT CCCCACTGCA CCTGGCTGTG
    CAGCAGGCCC ACGTGGGGCT GGTGCCACTG CTGGTGGATG CGGGCTGCAG CGTCAACGCC
    GAGGATGAGG AGGGGGACAC GGCTCTGCAC GTGGCCCTGC AGCGCCACCA GCTGCTGCCT
    CTGGCAGCTG ATGGGAGCGC AGGGGACCCG GGTCCCCTGC AGCTGCTGTC CAGGCTACAG
    GCCTCGGGCC TTCCGAGCAG TGCAGAGCTG ACGGCAGGCG CGGCTATGGC CTACTACTTA
    GCGCTGGAGG GCGCCGACCT GAGCTACGCC AACCACCGCG GCCGGAGCCC CCTGGACCTG
    GCTGCCGAGG GTCGCCTGCT CAAAGCCCTG CAGGGCTGTG CTCAGCGCTT CCGGGAGCGG
    CAGGCTGGAG GCAGTGGCAG CGGGGGCTCG GCCCAGGGTC CAAGACTGGT GCTCAGTGCT
    CCGAACACTG TAACCAACCT GCATGTGGCC GCGCCGACGG GCCCCGAGGC CGCTGAGTGC
    CTAGTGTGCT CGGAGCTGGC GCTGCTGGTG CTGTTCTCGC CCTGCCAGCA TCGCACAGTG
    TGCGAGGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG
    AAGCTCGCCC AGACGGCACG GAGGTGGTCA GCGCAGCCCC CGCGCCCGGC CCACCGCGGC
    AGTTGGTGGA GGAGCTGCAG AGCCGCTACA GGCAGATGGA GGAGCGCATC ACCTGTCCGA
    TTTGCATCGA CAGCCACATC CGCCTCGTGT TCCAGTGCGG CCACGGCGCG TGCGCGCCCT
    GTGGCGCGGC GCTCAGCGCC TGCCCGATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA
    TCTTCGTGTA GCCGGCGCCC CCCGCCTCGT TCCGGGGCCA TGCCCCGCGG CCGCCCTCAC
    GAGCCCTTTC TCTGTATTTT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94884
    Clone ID Related Accession (Same CDS sequence) XM_015466879.1
    Accession Version XM_015466879.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2688bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGGAGCAG GGCTGGCAGA GCATAGGGCC TGCCTCTGGC ACCACTGCCC TTCATGGCCT 
    GGGACCCACT CTGTTTCCCG TAGGGTCATG CTGAGTCCCC GCCAGGGCCT CCCAAGGATC
    CCGTTAAGGG GCATCTTCCA GGGGGCGAAG GTGGTTCGGG GCCCCGACTG GGAGTGGGGC
    TCACAGGATG GAGGAGAAGG GAAGCCAGGC CGAGTAGTGG ACATCCGTGG CTGGGACGTG
    GAGACAGGCC GGAGCGTGGC CAGTGTGACG TGGGCTGATG GCACCACCAA CGTGTACCGT
    GTGGGCCACA AGGGCAAGGT GGATCTCAAG TGTGTGGGCG AGGCAGCTGG CGGCTTCTAC
    TACAGGGACC ACCTCCCAAG GCTTGGCAAG CCCGCAGAGC TGCAGCGCAG GGTCAGTGCT
    GATGGCCAGC CCTTCCAGCG TGGGGACAAG GTCAAGTGTC TGCTGGACGC GGATGTGCTG
    AGGGAGATGC AGGAAGGCCA CGGCGGGTGG AACCCCCGGA TGGCCAAGTT TATCGGACAG
    ACGGGCACCG TGCACCGCAT CACAGACCGT GGGGACGTGC GTGTGCAGTT CAGCCACAAG
    ACCCGCTGGA CCTTCCACCC TGGGGCTCTC ACCAAGCAAA ACTCCTGCTT GGTGGGTGAG
    GTGGTGCGGG TTATCGATGA CCTTGACACG GTGAAGCGGC TGCAGGCTGG CCATGGGGAA
    TGGACCGATG ACATGGCCCC TGCTCTGGGC CGCATTGGGA GAGTGCTGAA GGTTTTCGGA
    GACGGGAACG TGGGTGTGCT GGTCAGTGGG AAGCTGTGGA CCTTTAGCCC CTCCTGCCTG
    GTGGCCTACC GGCCTGAGGA AGATGCCAAC CTGGACGTAG CTGGGCGAGC CCGGGAGAAC
    AAAAGCTCCC TGAGCGTTGT CTTGGACAAG CTTCGAGCCC AGAAGAGTGA CCTGGAGCAC
    CCAGGGAAGC TGGTGGTGGA GGTGGCTCTG GGCAATGTGG CCCGGGCTCT GGACCTGCTG
    CGGCGGCACC CAGAACAGGT GGACACCAAG AATCAGGGCA GGACTGCCCT GCAGATGGCT
    GCCTACCTAG GCCAGGTGGA GCTGGTGCGG CTGCTGCTGC AGGCTCAGGC GGGCGTTGAC
    CTGCCGGATG ATGAGGGCAA CACGGCACTG CACTACGCAG CCCTGGGGAA CCAGCCTGAG
    GCTGCCCGGG TGCTCTTGAG CTCAGGTTGT GGGGCCAATG CTCTGAACAG CACTCGGAGC
    TCAGCACTGC ATGTGGCCGT GCAGAGGGGC TTCCTGGAAG TGGTGAAGGT CCTGTGTGAG
    CGTGGCTGTG ACGTCAACCT GCCTGATGCC CATGCTGATA CACCCCTGCA CTGCGCCATC
    TCAGCGGGCA CTGGTGCCAG CGGCATTGTG GAGGTCCTCA CCGAGGTGCC AGGCATCGAT
    GTCACTGCCA CCAACAGCCA GGGCTTCACT CTGCTGCACC ATGCGTCCCT CAAAGGCCAC
    ACACTAGCTG TCAGGAGGAT TCTGGCACGG GCACGGCAGC TGGTGGACGC CAAGAAGGAG
    GATGGTTTTA CGGCACTGCA CCTGGCCGCC TTCAACAACC ATGGGGAGGT GGCCCAGGTT
    CTCATCCAAG AGGGCCACTG TGATGTGAAC GCTCGCAACC GTAAGCTGCA GTCCCCACTG
    CACCTGGCTG TGCAGCAGGC CCACGTGGGG CTGGTGCCAC TGCTGGTGGA TGCGGGCTGC
    AGCGTCAACG CCGAGGATGA GGAGGGGGAC ACGGCTCTGC ACGTGGCCCT GCAGCGCCAC
    CAGCTGCTGC CTCTGGCAGC TGATGGGAGC GCAGGGGACC CGGGTCCCCT GCAGCTGCTG
    TCCAGGCTAC AGGCCTCGGG CCTTCCGAGC AGTGCAGAGC TGACGGCAGG CGCGGCTATG
    GCCTACTACT TAGCGCTGGA GGGCGCCGAC CTGAGCTACG CCAACCACCG CGGCCGGAGC
    CCCCTGGACC TGGCTGCCGA GGGTCGCCTG CTCAAAGCCC TGCAGGGCTG TGCTCAGCGC
    TTCCGGGAGC GGCAGGCTGG AGGCAGTGGC AGCGGGGGCT CGGCCCAGGG TCCAAGACTG
    GTGCTCAGTG CTCCGAACAC TGTAACCAAC CTGCATGTGG CCGCGCCGAC GGGCCCCGAG
    GCCGCTGAGT GCCTAGTGTG CTCGGAGCTG GCGCTGCTGG TGCTGTTCTC GCCCTGCCAG
    CATCGCACAG TGTGCGAGGG TGAGTGGCCG GGTGGGGAGG GGGCCGCGGC CGGCTGGACG
    GCCCTCACTC AACCGTGCCC TCCCCCCGCA GAGTGCGCGC GCAGGATGAA GAAATGCATC
    AGGTGCCAGG TGGTCATCAG CAAGAAGCTC GCCCAGACGG CACGGAGGTG GTCAGCGCAG
    CCCCCGCGCC CGGCCCACCG CGGCAGTTGG TGGAGGAGCT GCAGAGCCGC TACAGGCAGA
    TGGAGGAGCG CATCACCTGT CCGATTTGCA TCGACAGCCA CATCCGCCTC GTGTTCCAGT
    GCGGCCACGG CGCGTGCGCG CCCTGTGGCG CGGCGCTCAG CGCCTGCCCG ATCTGCCGCC
    AGCCCATCCG CGACCGCATC CAGATCTTCG TGTAGCCGGC GCCCCCCGCC TCGTTCCGGG
    GCCATGCCCC GCGGCCGCCC TCACGAGCCC TTTCTCTGTA TTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322365.1
    CDS502..3189
    Translation

    Target ORF information:

    RefSeq Version XM_015466879.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466879.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGGAGCAG GGCTGGCAGA GCATAGGGCC TGCCTCTGGC ACCACTGCCC TTCATGGCCT 
    GGGACCCACT CTGTTTCCCG TAGGGTCATG CTGAGTCCCC GCCAGGGCCT CCCAAGGATC
    CCGTTAAGGG GCATCTTCCA GGGGGCGAAG GTGGTTCGGG GCCCCGACTG GGAGTGGGGC
    TCACAGGATG GAGGAGAAGG GAAGCCAGGC CGAGTAGTGG ACATCCGTGG CTGGGACGTG
    GAGACAGGCC GGAGCGTGGC CAGTGTGACG TGGGCTGATG GCACCACCAA CGTGTACCGT
    GTGGGCCACA AGGGCAAGGT GGATCTCAAG TGTGTGGGCG AGGCAGCTGG CGGCTTCTAC
    TACAGGGACC ACCTCCCAAG GCTTGGCAAG CCCGCAGAGC TGCAGCGCAG GGTCAGTGCT
    GATGGCCAGC CCTTCCAGCG TGGGGACAAG GTCAAGTGTC TGCTGGACGC GGATGTGCTG
    AGGGAGATGC AGGAAGGCCA CGGCGGGTGG AACCCCCGGA TGGCCAAGTT TATCGGACAG
    ACGGGCACCG TGCACCGCAT CACAGACCGT GGGGACGTGC GTGTGCAGTT CAGCCACAAG
    ACCCGCTGGA CCTTCCACCC TGGGGCTCTC ACCAAGCAAA ACTCCTGCTT GGTGGGTGAG
    GTGGTGCGGG TTATCGATGA CCTTGACACG GTGAAGCGGC TGCAGGCTGG CCATGGGGAA
    TGGACCGATG ACATGGCCCC TGCTCTGGGC CGCATTGGGA GAGTGCTGAA GGTTTTCGGA
    GACGGGAACG TGGGTGTGCT GGTCAGTGGG AAGCTGTGGA CCTTTAGCCC CTCCTGCCTG
    GTGGCCTACC GGCCTGAGGA AGATGCCAAC CTGGACGTAG CTGGGCGAGC CCGGGAGAAC
    AAAAGCTCCC TGAGCGTTGT CTTGGACAAG CTTCGAGCCC AGAAGAGTGA CCTGGAGCAC
    CCAGGGAAGC TGGTGGTGGA GGTGGCTCTG GGCAATGTGG CCCGGGCTCT GGACCTGCTG
    CGGCGGCACC CAGAACAGGT GGACACCAAG AATCAGGGCA GGACTGCCCT GCAGATGGCT
    GCCTACCTAG GCCAGGTGGA GCTGGTGCGG CTGCTGCTGC AGGCTCAGGC GGGCGTTGAC
    CTGCCGGATG ATGAGGGCAA CACGGCACTG CACTACGCAG CCCTGGGGAA CCAGCCTGAG
    GCTGCCCGGG TGCTCTTGAG CTCAGGTTGT GGGGCCAATG CTCTGAACAG CACTCGGAGC
    TCAGCACTGC ATGTGGCCGT GCAGAGGGGC TTCCTGGAAG TGGTGAAGGT CCTGTGTGAG
    CGTGGCTGTG ACGTCAACCT GCCTGATGCC CATGCTGATA CACCCCTGCA CTGCGCCATC
    TCAGCGGGCA CTGGTGCCAG CGGCATTGTG GAGGTCCTCA CCGAGGTGCC AGGCATCGAT
    GTCACTGCCA CCAACAGCCA GGGCTTCACT CTGCTGCACC ATGCGTCCCT CAAAGGCCAC
    ACACTAGCTG TCAGGAGGAT TCTGGCACGG GCACGGCAGC TGGTGGACGC CAAGAAGGAG
    GATGGTTTTA CGGCACTGCA CCTGGCCGCC TTCAACAACC ATGGGGAGGT GGCCCAGGTT
    CTCATCCAAG AGGGCCACTG TGATGTGAAC GCTCGCAACC GTAAGCTGCA GTCCCCACTG
    CACCTGGCTG TGCAGCAGGC CCACGTGGGG CTGGTGCCAC TGCTGGTGGA TGCGGGCTGC
    AGCGTCAACG CCGAGGATGA GGAGGGGGAC ACGGCTCTGC ACGTGGCCCT GCAGCGCCAC
    CAGCTGCTGC CTCTGGCAGC TGATGGGAGC GCAGGGGACC CGGGTCCCCT GCAGCTGCTG
    TCCAGGCTAC AGGCCTCGGG CCTTCCGAGC AGTGCAGAGC TGACGGCAGG CGCGGCTATG
    GCCTACTACT TAGCGCTGGA GGGCGCCGAC CTGAGCTACG CCAACCACCG CGGCCGGAGC
    CCCCTGGACC TGGCTGCCGA GGGTCGCCTG CTCAAAGCCC TGCAGGGCTG TGCTCAGCGC
    TTCCGGGAGC GGCAGGCTGG AGGCAGTGGC AGCGGGGGCT CGGCCCAGGG TCCAAGACTG
    GTGCTCAGTG CTCCGAACAC TGTAACCAAC CTGCATGTGG CCGCGCCGAC GGGCCCCGAG
    GCCGCTGAGT GCCTAGTGTG CTCGGAGCTG GCGCTGCTGG TGCTGTTCTC GCCCTGCCAG
    CATCGCACAG TGTGCGAGGG TGAGTGGCCG GGTGGGGAGG GGGCCGCGGC CGGCTGGACG
    GCCCTCACTC AACCGTGCCC TCCCCCCGCA GAGTGCGCGC GCAGGATGAA GAAATGCATC
    AGGTGCCAGG TGGTCATCAG CAAGAAGCTC GCCCAGACGG CACGGAGGTG GTCAGCGCAG
    CCCCCGCGCC CGGCCCACCG CGGCAGTTGG TGGAGGAGCT GCAGAGCCGC TACAGGCAGA
    TGGAGGAGCG CATCACCTGT CCGATTTGCA TCGACAGCCA CATCCGCCTC GTGTTCCAGT
    GCGGCCACGG CGCGTGCGCG CCCTGTGGCG CGGCGCTCAG CGCCTGCCCG ATCTGCCGCC
    AGCCCATCCG CGACCGCATC CAGATCTTCG TGTAGCCGGC GCCCCCCGCC TCGTTCCGGG
    GCCATGCCCC GCGGCCGCCC TCACGAGCCC TTTCTCTGTA TTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94885
    Clone ID Related Accession (Same CDS sequence) XM_015466880.1
    Accession Version XM_015466880.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2667bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC
    GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTCGCCCAG
    ACCTCAGCCC CGAGCCCCCG CCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322366.1
    CDS1594..4260
    Translation

    Target ORF information:

    RefSeq Version XM_015466880.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466880.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC
    GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTCGCCCAG
    ACCTCAGCCC CGAGCCCCCG CCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94886
    Clone ID Related Accession (Same CDS sequence) XM_015466881.1
    Accession Version XM_015466881.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2610bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCGGGTCA TGCTGAGTCC CCGCCAGGGC CTCCCAAGGA TCCCGTTAAG GGGCATCTTC 
    CAGGGGGCGA AGGTGGTTCG GGGCCCCGAC TGGGAGTGGG GCTCACAGGA TGGAGGAGAA
    GGGAAGCCAG GCCGAGTAGT GGACATCCGT GGCTGGGACG TGGAGACAGG CCGGAGCGTG
    GCCAGTGTGA CGTGGGCTGA TGGCACCACC AACGTGTACC GTGTGGGCCA CAAGGGCAAG
    GTGGATCTCA AGTGTGTGGG CGAGGCAGCT GGCGGCTTCT ACTACAGGGA CCACCTCCCA
    AGGCTTGGCA AGCCCGCAGA GCTGCAGCGC AGGGTCAGTG CTGATGGCCA GCCCTTCCAG
    CGTGGGGACA AGGTCAAGTG TCTGCTGGAC GCGGATGTGC TGAGGGAGAT GCAGGAAGGC
    CACGGCGGGT GGAACCCCCG GATGGCCAAG TTTATCGGAC AGACGGGCAC CGTGCACCGC
    ATCACAGACC GTGGGGACGT GCGTGTGCAG TTCAGCCACA AGACCCGCTG GACCTTCCAC
    CCTGGGGCTC TCACCAAGCA AAACTCCTGC TTGGTGGGTG AGGTGGTGCG GGTTATCGAT
    GACCTTGACA CGGTGAAGCG GCTGCAGGCT GGCCATGGGG AATGGACCGA TGACATGGCC
    CCTGCTCTGG GCCGCATTGG GAGAGTGCTG AAGGTTTTCG GAGACGGGAA CGTGGGTGTG
    CTGGTCAGTG GGAAGCTGTG GACCTTTAGC CCCTCCTGCC TGGTGGCCTA CCGGCCTGAG
    GAAGATGCCA ACCTGGACGT AGCTGGGCGA GCCCGGGAGA ACAAAAGCTC CCTGAGCGTT
    GTCTTGGACA AGCTTCGAGC CCAGAAGAGT GACCTGGAGC ACCCAGGGAA GCTGGTGGTG
    GAGGTGGCTC TGGGCAATGT GGCCCGGGCT CTGGACCTGC TGCGGCGGCA CCCAGAACAG
    GTGGACACCA AGAATCAGGG CAGGACTGCC CTGCAGATGG CTGCCTACCT AGGCCAGGTG
    GAGCTGGTGC GGCTGCTGCT GCAGGCTCAG GCGGGCGTTG ACCTGCCGGA TGATGAGGGC
    AACACGGCAC TGCACTACGC AGCCCTGGGG AACCAGCCTG AGGCTGCCCG GGTGCTCTTG
    AGCTCAGGTT GTGGGGCCAA TGCTCTGAAC AGCACTCGGA GCTCAGCACT GCATGTGGCC
    GTGCAGAGGG GCTTCCTGGA AGTGGTGAAG GTCCTGTGTG AGCGTGGCTG TGACGTCAAC
    CTGCCTGATG CCCATGCTGA TACACCCCTG CACTGCGCCA TCTCAGCGGG CACTGGTGCC
    AGCGGCATTG TGGAGGTCCT CACCGAGGTG CCAGGCATCG ATGTCACTGC CACCAACAGC
    CAGGGCTTCA CTCTGCTGCA CCATGCGTCC CTCAAAGGCC ACACACTAGC TGTCAGGAGG
    ATTCTGGCAC GGGCACGGCA GCTGGTGGAC GCCAAGAAGG AGGATGGTTT TACGGCACTG
    CACCTGGCCG CCTTCAACAA CCATGGGGAG GTGGCCCAGG TTCTCATCCA AGAGGGCCAC
    TGTGATGTGA ACGCTCGCAA CCGTAAGCTG CAGTCCCCAC TGCACCTGGC TGTGCAGCAG
    GCCCACGTGG GGCTGGTGCC ACTGCTGGTG GATGCGGGCT GCAGCGTCAA CGCCGAGGAT
    GAGGAGGGGG ACACGGCTCT GCACGTGGCC CTGCAGCGCC ACCAGCTGCT GCCTCTGGCA
    GCTGATGGGA GCGCAGGGGA CCCGGGTCCC CTGCAGCTGC TGTCCAGGCT ACAGGCCTCG
    GGCCTTCCGA GCAGTGCAGA GCTGACGGCA GGCGCGGCTA TGGCCTACTA CTTAGCGCTG
    GAGGGCGCCG ACCTGAGCTA CGCCAACCAC CGCGGCCGGA GCCCCCTGGA CCTGGCTGCC
    GAGGGTCGCC TGCTCAAAGC CCTGCAGGGC TGTGCTCAGC GCTTCCGGGA GCGGCAGGCT
    GGAGGCAGTG GCAGCGGGGG CTCGGCCCAG GGTCCAAGAC TGGTGCTCAG TGCTCCGAAC
    ACTGTAACCA ACCTGCATGT GGCCGCGCCG ACGGGCCCCG AGGCCGCTGA GTGCCTAGTG
    TGCTCGGAGC TGGCGCTGCT GGTGCTGTTC TCGCCCTGCC AGCATCGCAC AGTGTGCGAG
    GGTGAGTGGC CGGGTGGGGA GGGGGCCGCG GCCGGCTGGA CGGCCCTCAC TCAACCGTGC
    CCTCCCCCCG CAGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC
    AGCAAGAAGC TCGCCCAGAC GGCACGGAGG TGGTCAGCGC AGCCCCCGCG CCCGGCCCAC
    CGCGGCAGTT GGTGGAGGAG CTGCAGAGCC GCTACAGGCA GATGGAGGAG CGCATCACCT
    GTCCGATTTG CATCGACAGC CACATCCGCC TCGTGTTCCA GTGCGGCCAC GGCGCGTGCG
    CGCCCTGTGG CGCGGCGCTC AGCGCCTGCC CGATCTGCCG CCAGCCCATC CGCGACCGCA
    TCCAGATCTT CGTGTAGCCG GCGCCCCCCG CCTCGTTCCG GGGCCATGCC CCGCGGCCGC
    CCTCACGAGC CCTTTCTCTG TATTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322367.1
    CDS350..2959
    Translation

    Target ORF information:

    RefSeq Version XM_015466881.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466881.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCGGGTCA TGCTGAGTCC CCGCCAGGGC CTCCCAAGGA TCCCGTTAAG GGGCATCTTC 
    CAGGGGGCGA AGGTGGTTCG GGGCCCCGAC TGGGAGTGGG GCTCACAGGA TGGAGGAGAA
    GGGAAGCCAG GCCGAGTAGT GGACATCCGT GGCTGGGACG TGGAGACAGG CCGGAGCGTG
    GCCAGTGTGA CGTGGGCTGA TGGCACCACC AACGTGTACC GTGTGGGCCA CAAGGGCAAG
    GTGGATCTCA AGTGTGTGGG CGAGGCAGCT GGCGGCTTCT ACTACAGGGA CCACCTCCCA
    AGGCTTGGCA AGCCCGCAGA GCTGCAGCGC AGGGTCAGTG CTGATGGCCA GCCCTTCCAG
    CGTGGGGACA AGGTCAAGTG TCTGCTGGAC GCGGATGTGC TGAGGGAGAT GCAGGAAGGC
    CACGGCGGGT GGAACCCCCG GATGGCCAAG TTTATCGGAC AGACGGGCAC CGTGCACCGC
    ATCACAGACC GTGGGGACGT GCGTGTGCAG TTCAGCCACA AGACCCGCTG GACCTTCCAC
    CCTGGGGCTC TCACCAAGCA AAACTCCTGC TTGGTGGGTG AGGTGGTGCG GGTTATCGAT
    GACCTTGACA CGGTGAAGCG GCTGCAGGCT GGCCATGGGG AATGGACCGA TGACATGGCC
    CCTGCTCTGG GCCGCATTGG GAGAGTGCTG AAGGTTTTCG GAGACGGGAA CGTGGGTGTG
    CTGGTCAGTG GGAAGCTGTG GACCTTTAGC CCCTCCTGCC TGGTGGCCTA CCGGCCTGAG
    GAAGATGCCA ACCTGGACGT AGCTGGGCGA GCCCGGGAGA ACAAAAGCTC CCTGAGCGTT
    GTCTTGGACA AGCTTCGAGC CCAGAAGAGT GACCTGGAGC ACCCAGGGAA GCTGGTGGTG
    GAGGTGGCTC TGGGCAATGT GGCCCGGGCT CTGGACCTGC TGCGGCGGCA CCCAGAACAG
    GTGGACACCA AGAATCAGGG CAGGACTGCC CTGCAGATGG CTGCCTACCT AGGCCAGGTG
    GAGCTGGTGC GGCTGCTGCT GCAGGCTCAG GCGGGCGTTG ACCTGCCGGA TGATGAGGGC
    AACACGGCAC TGCACTACGC AGCCCTGGGG AACCAGCCTG AGGCTGCCCG GGTGCTCTTG
    AGCTCAGGTT GTGGGGCCAA TGCTCTGAAC AGCACTCGGA GCTCAGCACT GCATGTGGCC
    GTGCAGAGGG GCTTCCTGGA AGTGGTGAAG GTCCTGTGTG AGCGTGGCTG TGACGTCAAC
    CTGCCTGATG CCCATGCTGA TACACCCCTG CACTGCGCCA TCTCAGCGGG CACTGGTGCC
    AGCGGCATTG TGGAGGTCCT CACCGAGGTG CCAGGCATCG ATGTCACTGC CACCAACAGC
    CAGGGCTTCA CTCTGCTGCA CCATGCGTCC CTCAAAGGCC ACACACTAGC TGTCAGGAGG
    ATTCTGGCAC GGGCACGGCA GCTGGTGGAC GCCAAGAAGG AGGATGGTTT TACGGCACTG
    CACCTGGCCG CCTTCAACAA CCATGGGGAG GTGGCCCAGG TTCTCATCCA AGAGGGCCAC
    TGTGATGTGA ACGCTCGCAA CCGTAAGCTG CAGTCCCCAC TGCACCTGGC TGTGCAGCAG
    GCCCACGTGG GGCTGGTGCC ACTGCTGGTG GATGCGGGCT GCAGCGTCAA CGCCGAGGAT
    GAGGAGGGGG ACACGGCTCT GCACGTGGCC CTGCAGCGCC ACCAGCTGCT GCCTCTGGCA
    GCTGATGGGA GCGCAGGGGA CCCGGGTCCC CTGCAGCTGC TGTCCAGGCT ACAGGCCTCG
    GGCCTTCCGA GCAGTGCAGA GCTGACGGCA GGCGCGGCTA TGGCCTACTA CTTAGCGCTG
    GAGGGCGCCG ACCTGAGCTA CGCCAACCAC CGCGGCCGGA GCCCCCTGGA CCTGGCTGCC
    GAGGGTCGCC TGCTCAAAGC CCTGCAGGGC TGTGCTCAGC GCTTCCGGGA GCGGCAGGCT
    GGAGGCAGTG GCAGCGGGGG CTCGGCCCAG GGTCCAAGAC TGGTGCTCAG TGCTCCGAAC
    ACTGTAACCA ACCTGCATGT GGCCGCGCCG ACGGGCCCCG AGGCCGCTGA GTGCCTAGTG
    TGCTCGGAGC TGGCGCTGCT GGTGCTGTTC TCGCCCTGCC AGCATCGCAC AGTGTGCGAG
    GGTGAGTGGC CGGGTGGGGA GGGGGCCGCG GCCGGCTGGA CGGCCCTCAC TCAACCGTGC
    CCTCCCCCCG CAGAGTGCGC GCGCAGGATG AAGAAATGCA TCAGGTGCCA GGTGGTCATC
    AGCAAGAAGC TCGCCCAGAC GGCACGGAGG TGGTCAGCGC AGCCCCCGCG CCCGGCCCAC
    CGCGGCAGTT GGTGGAGGAG CTGCAGAGCC GCTACAGGCA GATGGAGGAG CGCATCACCT
    GTCCGATTTG CATCGACAGC CACATCCGCC TCGTGTTCCA GTGCGGCCAC GGCGCGTGCG
    CGCCCTGTGG CGCGGCGCTC AGCGCCTGCC CGATCTGCCG CCAGCCCATC CGCGACCGCA
    TCCAGATCTT CGTGTAGCCG GCGCCCCCCG CCTCGTTCCG GGGCCATGCC CCGCGGCCGC
    CCTCACGAGC CCTTTCTCTG TATTTTATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94887
    Clone ID Related Accession (Same CDS sequence) XM_015466882.1
    Accession Version XM_015466882.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2601bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTGAGTC CCCGCCAGGG CCTCCCAAGG ATCCCGTTAA GGGGCATCTT CCAGGGGGCG 
    AAGGTGGTTC GGGGCCCCGA CTGGGAGTGG GGCTCACAGG ATGGAGGAGA AGGGAAGCCA
    GGCCGAGTAG TGGACATCCG TGGCTGGGAC GTGGAGACAG GCCGGAGCGT GGCCAGTGTG
    ACGTGGGCTG ATGGCACCAC CAACGTGTAC CGTGTGGGCC ACAAGGGCAA GGTGGATCTC
    AAGTGTGTGG GCGAGGCAGC TGGCGGCTTC TACTACAGGG ACCACCTCCC AAGGCTTGGC
    AAGCCCGCAG AGCTGCAGCG CAGGGTCAGT GCTGATGGCC AGCCCTTCCA GCGTGGGGAC
    AAGGTCAAGT GTCTGCTGGA CGCGGATGTG CTGAGGGAGA TGCAGGAAGG CCACGGCGGG
    TGGAACCCCC GGATGGCCAA GTTTATCGGA CAGACGGGCA CCGTGCACCG CATCACAGAC
    CGTGGGGACG TGCGTGTGCA GTTCAGCCAC AAGACCCGCT GGACCTTCCA CCCTGGGGCT
    CTCACCAAGC AAAACTCCTG CTTGGTGGGT GAGGTGGTGC GGGTTATCGA TGACCTTGAC
    ACGGTGAAGC GGCTGCAGGC TGGCCATGGG GAATGGACCG ATGACATGGC CCCTGCTCTG
    GGCCGCATTG GGAGAGTGCT GAAGGTTTTC GGAGACGGGA ACGTGGGTGT GCTGGTCAGT
    GGGAAGCTGT GGACCTTTAG CCCCTCCTGC CTGGTGGCCT ACCGGCCTGA GGAAGATGCC
    AACCTGGACG TAGCTGGGCG AGCCCGGGAG AACAAAAGCT CCCTGAGCGT TGTCTTGGAC
    AAGCTTCGAG CCCAGAAGAG TGACCTGGAG CACCCAGGGA AGCTGGTGGT GGAGGTGGCT
    CTGGGCAATG TGGCCCGGGC TCTGGACCTG CTGCGGCGGC ACCCAGAACA GGTGGACACC
    AAGAATCAGG GCAGGACTGC CCTGCAGATG GCTGCCTACC TAGGCCAGGT GGAGCTGGTG
    CGGCTGCTGC TGCAGGCTCA GGCGGGCGTT GACCTGCCGG ATGATGAGGG CAACACGGCA
    CTGCACTACG CAGCCCTGGG GAACCAGCCT GAGGCTGCCC GGGTGCTCTT GAGCTCAGGT
    TGTGGGGCCA ATGCTCTGAA CAGCACTCGG AGCTCAGCAC TGCATGTGGC CGTGCAGAGG
    GGCTTCCTGG AAGTGGTGAA GGTCCTGTGT GAGCGTGGCT GTGACGTCAA CCTGCCTGAT
    GCCCATGCTG ATACACCCCT GCACTGCGCC ATCTCAGCGG GCACTGGTGC CAGCGGCATT
    GTGGAGGTCC TCACCGAGGT GCCAGGCATC GATGTCACTG CCACCAACAG CCAGGGCTTC
    ACTCTGCTGC ACCATGCGTC CCTCAAAGGC CACACACTAG CTGTCAGGAG GATTCTGGCA
    CGGGCACGGC AGCTGGTGGA CGCCAAGAAG GAGGATGGTT TTACGGCACT GCACCTGGCC
    GCCTTCAACA ACCATGGGGA GGTGGCCCAG GTTCTCATCC AAGAGGGCCA CTGTGATGTG
    AACGCTCGCA ACCGTAAGCT GCAGTCCCCA CTGCACCTGG CTGTGCAGCA GGCCCACGTG
    GGGCTGGTGC CACTGCTGGT GGATGCGGGC TGCAGCGTCA ACGCCGAGGA TGAGGAGGGG
    GACACGGCTC TGCACGTGGC CCTGCAGCGC CACCAGCTGC TGCCTCTGGC AGCTGATGGG
    AGCGCAGGGG ACCCGGGTCC CCTGCAGCTG CTGTCCAGGC TACAGGCCTC GGGCCTTCCG
    AGCAGTGCAG AGCTGACGGC AGGCGCGGCT ATGGCCTACT ACTTAGCGCT GGAGGGCGCC
    GACCTGAGCT ACGCCAACCA CCGCGGCCGG AGCCCCCTGG ACCTGGCTGC CGAGGGTCGC
    CTGCTCAAAG CCCTGCAGGG CTGTGCTCAG CGCTTCCGGG AGCGGCAGGC TGGAGGCAGT
    GGCAGCGGGG GCTCGGCCCA GGGTCCAAGA CTGGTGCTCA GTGCTCCGAA CACTGTAACC
    AACCTGCATG TGGCCGCGCC GACGGGCCCC GAGGCCGCTG AGTGCCTAGT GTGCTCGGAG
    CTGGCGCTGC TGGTGCTGTT CTCGCCCTGC CAGCATCGCA CAGTGTGCGA GGGTGAGTGG
    CCGGGTGGGG AGGGGGCCGC GGCCGGCTGG ACGGCCCTCA CTCAACCGTG CCCTCCCCCC
    GCAGAGTGCG CGCGCAGGAT GAAGAAATGC ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG
    CTCGCCCAGA CGGCACGGAG GTGGTCAGCG CAGCCCCCGC GCCCGGCCCA CCGCGGCAGT
    TGGTGGAGGA GCTGCAGAGC CGCTACAGGC AGATGGAGGA GCGCATCACC TGTCCGATTT
    GCATCGACAG CCACATCCGC CTCGTGTTCC AGTGCGGCCA CGGCGCGTGC GCGCCCTGTG
    GCGCGGCGCT CAGCGCCTGC CCGATCTGCC GCCAGCCCAT CCGCGACCGC ATCCAGATCT
    TCGTGTAGCC GGCGCCCCCC GCCTCGTTCC GGGGCCATGC CCCGCGGCCG CCCTCACGAG
    CCCTTTCTCT GTATTTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322368.1
    CDS262..2862
    Translation

    Target ORF information:

    RefSeq Version XM_015466882.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466882.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGCTGAGTC CCCGCCAGGG CCTCCCAAGG ATCCCGTTAA GGGGCATCTT CCAGGGGGCG 
    AAGGTGGTTC GGGGCCCCGA CTGGGAGTGG GGCTCACAGG ATGGAGGAGA AGGGAAGCCA
    GGCCGAGTAG TGGACATCCG TGGCTGGGAC GTGGAGACAG GCCGGAGCGT GGCCAGTGTG
    ACGTGGGCTG ATGGCACCAC CAACGTGTAC CGTGTGGGCC ACAAGGGCAA GGTGGATCTC
    AAGTGTGTGG GCGAGGCAGC TGGCGGCTTC TACTACAGGG ACCACCTCCC AAGGCTTGGC
    AAGCCCGCAG AGCTGCAGCG CAGGGTCAGT GCTGATGGCC AGCCCTTCCA GCGTGGGGAC
    AAGGTCAAGT GTCTGCTGGA CGCGGATGTG CTGAGGGAGA TGCAGGAAGG CCACGGCGGG
    TGGAACCCCC GGATGGCCAA GTTTATCGGA CAGACGGGCA CCGTGCACCG CATCACAGAC
    CGTGGGGACG TGCGTGTGCA GTTCAGCCAC AAGACCCGCT GGACCTTCCA CCCTGGGGCT
    CTCACCAAGC AAAACTCCTG CTTGGTGGGT GAGGTGGTGC GGGTTATCGA TGACCTTGAC
    ACGGTGAAGC GGCTGCAGGC TGGCCATGGG GAATGGACCG ATGACATGGC CCCTGCTCTG
    GGCCGCATTG GGAGAGTGCT GAAGGTTTTC GGAGACGGGA ACGTGGGTGT GCTGGTCAGT
    GGGAAGCTGT GGACCTTTAG CCCCTCCTGC CTGGTGGCCT ACCGGCCTGA GGAAGATGCC
    AACCTGGACG TAGCTGGGCG AGCCCGGGAG AACAAAAGCT CCCTGAGCGT TGTCTTGGAC
    AAGCTTCGAG CCCAGAAGAG TGACCTGGAG CACCCAGGGA AGCTGGTGGT GGAGGTGGCT
    CTGGGCAATG TGGCCCGGGC TCTGGACCTG CTGCGGCGGC ACCCAGAACA GGTGGACACC
    AAGAATCAGG GCAGGACTGC CCTGCAGATG GCTGCCTACC TAGGCCAGGT GGAGCTGGTG
    CGGCTGCTGC TGCAGGCTCA GGCGGGCGTT GACCTGCCGG ATGATGAGGG CAACACGGCA
    CTGCACTACG CAGCCCTGGG GAACCAGCCT GAGGCTGCCC GGGTGCTCTT GAGCTCAGGT
    TGTGGGGCCA ATGCTCTGAA CAGCACTCGG AGCTCAGCAC TGCATGTGGC CGTGCAGAGG
    GGCTTCCTGG AAGTGGTGAA GGTCCTGTGT GAGCGTGGCT GTGACGTCAA CCTGCCTGAT
    GCCCATGCTG ATACACCCCT GCACTGCGCC ATCTCAGCGG GCACTGGTGC CAGCGGCATT
    GTGGAGGTCC TCACCGAGGT GCCAGGCATC GATGTCACTG CCACCAACAG CCAGGGCTTC
    ACTCTGCTGC ACCATGCGTC CCTCAAAGGC CACACACTAG CTGTCAGGAG GATTCTGGCA
    CGGGCACGGC AGCTGGTGGA CGCCAAGAAG GAGGATGGTT TTACGGCACT GCACCTGGCC
    GCCTTCAACA ACCATGGGGA GGTGGCCCAG GTTCTCATCC AAGAGGGCCA CTGTGATGTG
    AACGCTCGCA ACCGTAAGCT GCAGTCCCCA CTGCACCTGG CTGTGCAGCA GGCCCACGTG
    GGGCTGGTGC CACTGCTGGT GGATGCGGGC TGCAGCGTCA ACGCCGAGGA TGAGGAGGGG
    GACACGGCTC TGCACGTGGC CCTGCAGCGC CACCAGCTGC TGCCTCTGGC AGCTGATGGG
    AGCGCAGGGG ACCCGGGTCC CCTGCAGCTG CTGTCCAGGC TACAGGCCTC GGGCCTTCCG
    AGCAGTGCAG AGCTGACGGC AGGCGCGGCT ATGGCCTACT ACTTAGCGCT GGAGGGCGCC
    GACCTGAGCT ACGCCAACCA CCGCGGCCGG AGCCCCCTGG ACCTGGCTGC CGAGGGTCGC
    CTGCTCAAAG CCCTGCAGGG CTGTGCTCAG CGCTTCCGGG AGCGGCAGGC TGGAGGCAGT
    GGCAGCGGGG GCTCGGCCCA GGGTCCAAGA CTGGTGCTCA GTGCTCCGAA CACTGTAACC
    AACCTGCATG TGGCCGCGCC GACGGGCCCC GAGGCCGCTG AGTGCCTAGT GTGCTCGGAG
    CTGGCGCTGC TGGTGCTGTT CTCGCCCTGC CAGCATCGCA CAGTGTGCGA GGGTGAGTGG
    CCGGGTGGGG AGGGGGCCGC GGCCGGCTGG ACGGCCCTCA CTCAACCGTG CCCTCCCCCC
    GCAGAGTGCG CGCGCAGGAT GAAGAAATGC ATCAGGTGCC AGGTGGTCAT CAGCAAGAAG
    CTCGCCCAGA CGGCACGGAG GTGGTCAGCG CAGCCCCCGC GCCCGGCCCA CCGCGGCAGT
    TGGTGGAGGA GCTGCAGAGC CGCTACAGGC AGATGGAGGA GCGCATCACC TGTCCGATTT
    GCATCGACAG CCACATCCGC CTCGTGTTCC AGTGCGGCCA CGGCGCGTGC GCGCCCTGTG
    GCGCGGCGCT CAGCGCCTGC CCGATCTGCC GCCAGCCCAT CCGCGACCGC ATCCAGATCT
    TCGTGTAGCC GGCGCCCCCC GCCTCGTTCC GGGGCCATGC CCCGCGGCCG CCCTCACGAG
    CCCTTTCTCT GTATTTTATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94888
    Clone ID Related Accession (Same CDS sequence) XM_015466883.1
    Accession Version XM_015466883.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2889bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC
    GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTCGCCCAG
    GTGGGGCCCG ACGGCACGGA GGTGGTCAGC GCAGCCCCCG CGCCCGGCCC ACCGCGGCAG
    TTGGTGGAGG AGCTGCAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGTCCGATT
    TGCATCGACA GCCACATCCG CCTCGTGTTC CAGTGCGGCC ACGGCGCGTG CGCGCCCTGT
    GGCGCGGCGC TCAGCGCCTG CCCGATCTGC CGCCAGCCCA TCCGCGACCG CATCCAGATC
    TTCGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322369.1
    CDS1594..4482
    Translation

    Target ORF information:

    RefSeq Version XM_015466883.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466883.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGAGTGC
    GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTCGCCCAG
    GTGGGGCCCG ACGGCACGGA GGTGGTCAGC GCAGCCCCCG CGCCCGGCCC ACCGCGGCAG
    TTGGTGGAGG AGCTGCAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGTCCGATT
    TGCATCGACA GCCACATCCG CCTCGTGTTC CAGTGCGGCC ACGGCGCGTG CGCGCCCTGT
    GGCGCGGCGC TCAGCGCCTG CCCGATCTGC CGCCAGCCCA TCCGCGACCG CATCCAGATC
    TTCGTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94878
    Clone ID Related Accession (Same CDS sequence) XM_015466873.1
    Accession Version XM_015466873.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3024bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGGTGAG
    TGGCCGGGTG GGGAGGGGGC CGCGGCCGGC TGGACGGCCC TCACTCAACC GTGCCCTCCC
    CCCGCAGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG
    AAGCTCGCCC AGACGGCACG GAGGTGGTCA GCGCAGCCCC CGCGCCCGGC CCACCGCGGC
    AGTTGGTGGA GGAGCTGCAG AGCCGCTACA GGCAGATGGA GGAGCGCATC ACCTGTCCGA
    TTTGCATCGA CAGCCACATC CGCCTCGTGT TCCAGTGCGG CCACGGCGCG TGCGCGCCCT
    GTGGCGCGGC GCTCAGCGCC TGCCCGATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA
    TCTTCGTGTA GCCGGCGCCC CCCGCCTCGT TCCGGGGCCA TGCCCCGCGG CCGCCCTCAC
    GAGCCCTTTC TCTGTATTTT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322359.1
    CDS460..3483
    Translation

    Target ORF information:

    RefSeq Version XM_015466873.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466873.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGTTTATC GGACAGACGG GCACCGTGCA CCGCATCACA
    GACCGTGGGG ACGTGCGTGT GCAGTTCAGC CACAAGACCC GCTGGACCTT CCACCCTGGG
    GCTCTCACCA AGCAAAACTC CTGCTTGGTG GGTGAGGTGG TGCGGGTTAT CGATGACCTT
    GACACGGTGA AGCGGCTGCA GGCTGGCCAT GGGGAATGGA CCGATGACAT GGCCCCTGCT
    CTGGGCCGCA TTGGGAGAGT GCTGAAGGTT TTCGGAGACG GGAACGTGGG TGTGCTGGTC
    AGTGGGAAGC TGTGGACCTT TAGCCCCTCC TGCCTGGTGG CCTACCGGCC TGAGGAAGAT
    GCCAACCTGG ACGTAGCTGG GCGAGCCCGG GAGAACAAAA GCTCCCTGAG CGTTGTCTTG
    GACAAGCTTC GAGCCCAGAA GAGTGACCTG GAGCACCCAG GGAAGCTGGT GGTGGAGGTG
    GCTCTGGGCA ATGTGGCCCG GGCTCTGGAC CTGCTGCGGC GGCACCCAGA ACAGGTGGAC
    ACCAAGAATC AGGGCAGGAC TGCCCTGCAG ATGGCTGCCT ACCTAGGCCA GGTGGAGCTG
    GTGCGGCTGC TGCTGCAGGC TCAGGCGGGC GTTGACCTGC CGGATGATGA GGGCAACACG
    GCACTGCACT ACGCAGCCCT GGGGAACCAG CCTGAGGCTG CCCGGGTGCT CTTGAGCTCA
    GGTTGTGGGG CCAATGCTCT GAACAGCACT CGGAGCTCAG CACTGCATGT GGCCGTGCAG
    AGGGGCTTCC TGGAAGTGGT GAAGGTCCTG TGTGAGCGTG GCTGTGACGT CAACCTGCCT
    GATGCCCATG CTGATACACC CCTGCACTGC GCCATCTCAG CGGGCACTGG TGCCAGCGGC
    ATTGTGGAGG TCCTCACCGA GGTGCCAGGC ATCGATGTCA CTGCCACCAA CAGCCAGGGC
    TTCACTCTGC TGCACCATGC GTCCCTCAAA GGCCACACAC TAGCTGTCAG GAGGATTCTG
    GCACGGGCAC GGCAGCTGGT GGACGCCAAG AAGGAGGATG GTTTTACGGC ACTGCACCTG
    GCCGCCTTCA ACAACCATGG GGAGGTGGCC CAGGTTCTCA TCCAAGAGGG CCACTGTGAT
    GTGAACGCTC GCAACCGTAA GCTGCAGTCC CCACTGCACC TGGCTGTGCA GCAGGCCCAC
    GTGGGGCTGG TGCCACTGCT GGTGGATGCG GGCTGCAGCG TCAACGCCGA GGATGAGGAG
    GGGGACACGG CTCTGCACGT GGCCCTGCAG CGCCACCAGC TGCTGCCTCT GGCAGCTGAT
    GGGAGCGCAG GGGACCCGGG TCCCCTGCAG CTGCTGTCCA GGCTACAGGC CTCGGGCCTT
    CCGAGCAGTG CAGAGCTGAC GGCAGGCGCG GCTATGGCCT ACTACTTAGC GCTGGAGGGC
    GCCGACCTGA GCTACGCCAA CCACCGCGGC CGGAGCCCCC TGGACCTGGC TGCCGAGGGT
    CGCCTGCTCA AAGCCCTGCA GGGCTGTGCT CAGCGCTTCC GGGAGCGGCA GGCTGGAGGC
    AGTGGCAGCG GGGGCTCGGC CCAGGGTCCA AGACTGGTGC TCAGTGCTCC GAACACTGTA
    ACCAACCTGC ATGTGGCCGC GCCGACGGGC CCCGAGGCCG CTGAGTGCCT AGTGTGCTCG
    GAGCTGGCGC TGCTGGTGCT GTTCTCGCCC TGCCAGCATC GCACAGTGTG CGAGGGTGAG
    TGGCCGGGTG GGGAGGGGGC CGCGGCCGGC TGGACGGCCC TCACTCAACC GTGCCCTCCC
    CCCGCAGAGT GCGCGCGCAG GATGAAGAAA TGCATCAGGT GCCAGGTGGT CATCAGCAAG
    AAGCTCGCCC AGACGGCACG GAGGTGGTCA GCGCAGCCCC CGCGCCCGGC CCACCGCGGC
    AGTTGGTGGA GGAGCTGCAG AGCCGCTACA GGCAGATGGA GGAGCGCATC ACCTGTCCGA
    TTTGCATCGA CAGCCACATC CGCCTCGTGT TCCAGTGCGG CCACGGCGCG TGCGCGCCCT
    GTGGCGCGGC GCTCAGCGCC TGCCCGATCT GCCGCCAGCC CATCCGCGAC CGCATCCAGA
    TCTTCGTGTA GCCGGCGCCC CCCGCCTCGT TCCGGGGCCA TGCCCCGCGG CCGCCCTCAC
    GAGCCCTTTC TCTGTATTTT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa94879
    Clone ID Related Accession (Same CDS sequence) XM_015466874.1
    Accession Version XM_015466874.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3012bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase MIB2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014646324.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC
    GTGCGTGTGC AGTTCAGCCA CAAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG
    CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ATGACCTTGA CACGGTGAAG
    CGGCTGCAGG CTGGCCATGG GGAATGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT
    GGGAGAGTGC TGAAGGTTTT CGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG
    TGGACCTTTA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC
    GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGCG TTGTCTTGGA CAAGCTTCGA
    GCCCAGAAGA GTGACCTGGA GCACCCAGGG AAGCTGGTGG TGGAGGTGGC TCTGGGCAAT
    GTGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG
    GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGCCAGG TGGAGCTGGT GCGGCTGCTG
    CTGCAGGCTC AGGCGGGCGT TGACCTGCCG GATGATGAGG GCAACACGGC ACTGCACTAC
    GCAGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCAGG TTGTGGGGCC
    AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CCGTGCAGAG GGGCTTCCTG
    GAAGTGGTGA AGGTCCTGTG TGAGCGTGGC TGTGACGTCA ACCTGCCTGA TGCCCATGCT
    GATACACCCC TGCACTGCGC CATCTCAGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC
    CTCACCGAGG TGCCAGGCAT CGATGTCACT GCCACCAACA GCCAGGGCTT CACTCTGCTG
    CACCATGCGT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC ACGGGCACGG
    CAGCTGGTGG ACGCCAAGAA GGAGGATGGT TTTACGGCAC TGCACCTGGC CGCCTTCAAC
    AACCATGGGG AGGTGGCCCA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC
    AACCGTAAGC TGCAGTCCCC ACTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG
    CCACTGCTGG TGGATGCGGG CTGCAGCGTC AACGCCGAGG ATGAGGAGGG GGACACGGCT
    CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG CAGCTGATGG GAGCGCAGGG
    GACCCGGGTC CCCTGCAGCT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC GAGCAGTGCA
    GAGCTGACGG CAGGCGCGGC TATGGCCTAC TACTTAGCGC TGGAGGGCGC CGACCTGAGC
    TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA
    GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTGGAGGCAG TGGCAGCGGG
    GGCTCGGCCC AGGGTCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTAAC CAACCTGCAT
    GTGGCCGCGC CGACGGGCCC CGAGGCCGCT GAGTGCCTAG TGTGCTCGGA GCTGGCGCTG
    CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGGTGAGTG GCCGGGTGGG
    GAGGGGGCCG CGGCCGGCTG GACGGCCCTC ACTCAACCGT GCCCTCCCCC CGCAGAGTGC
    GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTCGCCCAG
    ACGGCACGGA GGTGGTCAGC GCAGCCCCCG CGCCCGGCCC ACCGCGGCAG TTGGTGGAGG
    AGCTGCAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGTCCGATT TGCATCGACA
    GCCACATCCG CCTCGTGTTC CAGTGCGGCC ACGGCGCGTG CGCGCCCTGT GGCGCGGCGC
    TCAGCGCCTG CCCGATCTGC CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGTAGC
    CGGCGCCCCC CGCCTCGTTC CGGGGCCATG CCCCGCGGCC GCCCTCACGA GCCCTTTCTC
    TGTATTTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015322360.1
    CDS1594..4605
    Translation

    Target ORF information:

    RefSeq Version XM_015466874.1
    Organism Bos taurus(cattle)
    Definition Bos taurus mindbomb E3 ubiquitin protein ligase 2 (MIB2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015466874.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGACCCAG ACCCCCAAGC AGGCGTGCAG GTGGGCATGC GCGTGGTGCG TGGCGTGGAC 
    TGGAAGTGGG GCCAGCAGGA CGGCGGCGAG GGCGGCGTGG GCACGGTGGT GGAGCTCGGC
    CGCCACGGCA GCCCTTCGAC CCCCGACCGC ACGGTGGTCG TGCAGTGGGA CCACGGCACT
    CGTACCAACT ACCGCGCGGG CTACCAGGGC GCACACGACC TGCTGCTCTA TGACAACGCC
    CAAATCGGTG TCCGCCACCC CAACATCATC TGCGACTGCT GCAAGAAGCA CGGGCTGCGG
    GGCATGCGCT GGAAGTGCCG TATCTGCTTC GACTACGATC TCTGCACGCA ATGCTACATG
    CACAACAAAC ACGACCTGGC CCACGCCTTC GAGCGCTACG AGACGGCCCA CTCGCGCCCG
    GTCATGCTGA GTCCCCGCCA GGGCCTCCCA AGGATCCCGT TAAGGGGCAT CTTCCAGGGG
    GCGAAGGTGG TTCGGGGCCC CGACTGGGAG TGGGGCTCAC AGGATGGAGG AGAAGGGAAG
    CCAGGCCGAG TAGTGGACAT CCGTGGCTGG GACGTGGAGA CAGGCCGGAG CGTGGCCAGT
    GTGACGTGGG CTGATGGCAC CACCAACGTG TACCGTGTGG GCCACAAGGG CAAGGTGGAT
    CTCAAGTGTG TGGGCGAGGC AGCTGGCGGC TTCTACTACA GGGACCACCT CCCAAGGCTT
    GGCAAGCCCG CAGAGCTGCA GCGCAGGGTC AGTGCTGATG GCCAGCCCTT CCAGCGTGGG
    GACAAGGTCA AGTGTCTGCT GGACGCGGAT GTGCTGAGGG AGATGCAGGA AGGCCACGGC
    GGGTGGAACC CCCGGATGGC CAAGACGGGC ACCGTGCACC GCATCACAGA CCGTGGGGAC
    GTGCGTGTGC AGTTCAGCCA CAAGACCCGC TGGACCTTCC ACCCTGGGGC TCTCACCAAG
    CAAAACTCCT GCTTGGTGGG TGAGGTGGTG CGGGTTATCG ATGACCTTGA CACGGTGAAG
    CGGCTGCAGG CTGGCCATGG GGAATGGACC GATGACATGG CCCCTGCTCT GGGCCGCATT
    GGGAGAGTGC TGAAGGTTTT CGGAGACGGG AACGTGGGTG TGCTGGTCAG TGGGAAGCTG
    TGGACCTTTA GCCCCTCCTG CCTGGTGGCC TACCGGCCTG AGGAAGATGC CAACCTGGAC
    GTAGCTGGGC GAGCCCGGGA GAACAAAAGC TCCCTGAGCG TTGTCTTGGA CAAGCTTCGA
    GCCCAGAAGA GTGACCTGGA GCACCCAGGG AAGCTGGTGG TGGAGGTGGC TCTGGGCAAT
    GTGGCCCGGG CTCTGGACCT GCTGCGGCGG CACCCAGAAC AGGTGGACAC CAAGAATCAG
    GGCAGGACTG CCCTGCAGAT GGCTGCCTAC CTAGGCCAGG TGGAGCTGGT GCGGCTGCTG
    CTGCAGGCTC AGGCGGGCGT TGACCTGCCG GATGATGAGG GCAACACGGC ACTGCACTAC
    GCAGCCCTGG GGAACCAGCC TGAGGCTGCC CGGGTGCTCT TGAGCTCAGG TTGTGGGGCC
    AATGCTCTGA ACAGCACTCG GAGCTCAGCA CTGCATGTGG CCGTGCAGAG GGGCTTCCTG
    GAAGTGGTGA AGGTCCTGTG TGAGCGTGGC TGTGACGTCA ACCTGCCTGA TGCCCATGCT
    GATACACCCC TGCACTGCGC CATCTCAGCG GGCACTGGTG CCAGCGGCAT TGTGGAGGTC
    CTCACCGAGG TGCCAGGCAT CGATGTCACT GCCACCAACA GCCAGGGCTT CACTCTGCTG
    CACCATGCGT CCCTCAAAGG CCACACACTA GCTGTCAGGA GGATTCTGGC ACGGGCACGG
    CAGCTGGTGG ACGCCAAGAA GGAGGATGGT TTTACGGCAC TGCACCTGGC CGCCTTCAAC
    AACCATGGGG AGGTGGCCCA GGTTCTCATC CAAGAGGGCC ACTGTGATGT GAACGCTCGC
    AACCGTAAGC TGCAGTCCCC ACTGCACCTG GCTGTGCAGC AGGCCCACGT GGGGCTGGTG
    CCACTGCTGG TGGATGCGGG CTGCAGCGTC AACGCCGAGG ATGAGGAGGG GGACACGGCT
    CTGCACGTGG CCCTGCAGCG CCACCAGCTG CTGCCTCTGG CAGCTGATGG GAGCGCAGGG
    GACCCGGGTC CCCTGCAGCT GCTGTCCAGG CTACAGGCCT CGGGCCTTCC GAGCAGTGCA
    GAGCTGACGG CAGGCGCGGC TATGGCCTAC TACTTAGCGC TGGAGGGCGC CGACCTGAGC
    TACGCCAACC ACCGCGGCCG GAGCCCCCTG GACCTGGCTG CCGAGGGTCG CCTGCTCAAA
    GCCCTGCAGG GCTGTGCTCA GCGCTTCCGG GAGCGGCAGG CTGGAGGCAG TGGCAGCGGG
    GGCTCGGCCC AGGGTCCAAG ACTGGTGCTC AGTGCTCCGA ACACTGTAAC CAACCTGCAT
    GTGGCCGCGC CGACGGGCCC CGAGGCCGCT GAGTGCCTAG TGTGCTCGGA GCTGGCGCTG
    CTGGTGCTGT TCTCGCCCTG CCAGCATCGC ACAGTGTGCG AGGGTGAGTG GCCGGGTGGG
    GAGGGGGCCG CGGCCGGCTG GACGGCCCTC ACTCAACCGT GCCCTCCCCC CGCAGAGTGC
    GCGCGCAGGA TGAAGAAATG CATCAGGTGC CAGGTGGTCA TCAGCAAGAA GCTCGCCCAG
    ACGGCACGGA GGTGGTCAGC GCAGCCCCCG CGCCCGGCCC ACCGCGGCAG TTGGTGGAGG
    AGCTGCAGAG CCGCTACAGG CAGATGGAGG AGCGCATCAC CTGTCCGATT TGCATCGACA
    GCCACATCCG CCTCGTGTTC CAGTGCGGCC ACGGCGCGTG CGCGCCCTGT GGCGCGGCGC
    TCAGCGCCTG CCCGATCTGC CGCCAGCCCA TCCGCGACCG CATCCAGATC TTCGTGTAGC
    CGGCGCCCCC CGCCTCGTTC CGGGGCCATG CCCCGCGGCC GCCCTCACGA GCCCTTTCTC
    TGTATTTTAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.