G6PC cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	G6PC
Entrez Gene ID	403492
Full Name	glucose-6-phosphatase catalytic subunit
Synonyms	G6Pase
General protein information	Preferred Names glucose-6-phosphatase catalytic subunit Names glucose-6-phosphatase glucose-6-phosphatase, catalytic (glycogen storage disease type I, von Gierke disease)
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_022422515.1	XP_022278223.1	glucose-6-phosphatase isoform X1
NM_001002993.2	NP_001002993.2	glucose-6-phosphatase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	G6PC	XP_003315515.1
Danio rerio (zebrafish)	g6pca.2	NP_001157278.1
Homo sapiens (human)	G6PC	NP_000142.2
Xenopus tropicalis (tropical clawed frog)	g6pc2	NP_001096531.1
	G6PC	XP_001112333.1
Gallus gallus (chicken)	LOC100857298	XP_003642865.1
Canis lupus familiaris (dog)	G6PC	NP_001002993.1
Xenopus tropicalis (tropical clawed frog)	g6pc	NP_001005682.1
Bos taurus (cattle)	G6PC	NP_001069592.1
Mus musculus (house mouse)	G6pc	NP_032087.2
Rattus norvegicus (Norway rat)	G6pc	NP_037230.2

Gene Ontology
Bibliography

Related articles in PubMed

Selective stimulation of G-6-Pase catalytic subunit but not G-6-P transporter gene expression by glucagon in vivo and cAMP in situ.
Hornbuckle LA, Everett CA, Martin CC, Gustavson SS, Svitek CA, Oeser JK, Neal DW, Cherrington AD, O'Brien RM
American journal of physiology. Endocrinology and metabolism286(5)E795-808(2004 May)

Selective tonic inhibition of G-6-Pase catalytic subunit, but not G-6-P transporter, gene expression by insulin in vivo.
Hornbuckle LA, Edgerton DS, Ayala JE, Svitek CA, Oeser JK, Neal DW, Cardin S, Cherrington AD, O'Brien RM
American journal of physiology. Endocrinology and metabolism281(4)E713-25(2001 Oct)

Isolation and nucleotide sequence of canine glucose-6-phosphatase mRNA: identification of mutation in puppies with glycogen storage disease type Ia.
Kishnani PS, Bao Y, Wu JY, Brix AE, Lin JL, Chen YT
Biochemical and molecular medicine61(2)168-77(1997 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Glucagon induces G-6-Pase catalytic subunit gene expression in the absence of insulin. Additionally, cAMP was shown to stimulate endogenous G-6-Pase catalytic subunit gene expression in HepG2 cells.
Title: Selective stimulation of G-6-Pase catalytic subunit but not G-6-P transporter gene expression by glucagon in vivo and cAMP in situ.

The following G6PC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the G6PC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCe11461	NM_001002993.2 Latest version!	Canis lupus familiaris glucose-6-phosphatase catalytic subunit (G6PC), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OCe11461	XM_022422515.1 Latest version!	Canis lupus familiaris glucose-6-phosphatase catalytic subunit (G6PC), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

G6PC ( NM_001002993.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> NP_001002993.2 Canis lupus familiaris glucose-6-phosphatase catalytic subunit (G6PC), mRNA.

Price & Availability↑

CloneID	OCe11461
Clone ID Related Accession (Same CDS sequence)	XM_022422515.1 , NM_001002993.2
Accession Version	NM_001002993.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-28
Organism	Canis lupus familiaris(dog)
Product	glucose-6-phosphatase
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AAEX03006440.1. On or before Sep 2, 2015 this sequence version replaced XM_005624319.1, NM_001002993.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: U91844.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00013515, SAMN00013638 [ECO:0000348] ##Evidence-Data-END##

ATGGAGAAAG GAATGGATGT GCTCCATGAC TTTGGAATCC AGTCAACGCA CTACCTCCAG 
GTGAATTACC AGGACTCTCA GGATTGGTTC ATCTTGGTGT CCGTGATTGC AGACCTCAGG 
AATGCCTTCT ATGTCCTCTT TCCCATCTGG TTCCATCTGC GTGAAGCTGT GGGCATCAAA 
CTTCTCTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAATG GATTCTGTTT 
GGACAGCGTC CATACTGGTG GGTCATGGAC ACCGACTACT ATAGCAACAC CTCTGTGCCA 
CTGATAAAGC AATTTCCAGT TACCTGTGAA ACTGGACCAG GGAGTCCCTC TGGTCATGCC 
ATGGGTACAG CAGGTGTATA CTACGTGATG GTCACATCTA CCCTTTCTAT CTTTCGGGGG 
AGAAAAAGGC CAACCTACAG ATTTCGGTGC TTGAATATCC TTCTGTGGTT GGGATTCTGG 
GCTGTGCAGC TGAACGTCTG TCTGTCCCGA ATCTACCTTG CTGCTCATTT CCCCCATCAG 
GTTGTTGCTG GAGTCCTGTC AGGCATTGCT GTTGCTGAAA CTTTCCGCCA CATCCAGAGC 
ATCTACAATG CCAGCCTCAA GAAATATTTT CTCATTACTT TCTTCCTGTT CAGTTTTGCC 
ATTGGATTTT ACCTGCTGCT CAAGGGGCTG GGTGTGGACC TCCTGTGGAC ACTGGAAAAA 
GCCAGGAGAT GGTGTGAGCG GCCGGAATGG GTTCACATTG ACACCACACC CTTTGCCAGC 
CTTCTCAAGA ACGTGGGGAC CCTCTTTGGC CTGGGGGTGG CTCTCAACTC CAGCATGTAC 
AGGGAAAGCT GCAAGGGCAA GCTTAGCAAG TGGTTCCCAT TCCGCCTCAG CTGCATTGTG 
GTGTCTCTCA TCCTCCTGCA CCTCTTTGAC TCTTTGAAAC CCCCATCCCA AACTGAGCTG 
ATCTTCTACA CCTTGTCCTT CTGCAAGAGT GCAGCAGTGC CCCTGGCATC TGTCAGCCTC 
ATCCCCTACT GCCTTGCCCG GGTCTTCGAC CAGCCAGACA AGAAGTCTTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001002993.2
CDS	66..1139
Translation	MEKGMDVLHDFGIQSTHYLQVNYQDSQDWFILVSVIADLRNAFYVLFPIWFHLREAVGIKLLWVAVIGDWLNLVFKWILFGQRPYWWVMDTDYYSNTSVPLIKQFPVTCETGPGSPSGHAMGTAGVYYVMVTSTLSIFRGRKRPTYRFRCLNILLWLGFWAVQLNVCLSRIYLAAHFPHQVVAGVLSGIAVAETFRHIQSIYNASLKKYFLITFFLFSFAIGFYLLLKGLGVDLLWTLEKARRWCERPEWVHIDTTPFASLLKNVGTLFGLGVALNSSMYRESCKGKLSKWFPFRLSCIVVSLILLHLFDSLKPPSQTELIFYTLSFCKSAAVPLASVSLIPYCLARVFDQPDKKSL

Target ORF information:

RefSeq Version	NM_001002993.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucose-6-phosphatase catalytic subunit (G6PC), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001002993.2

ORF Insert Sequence:

ATGGAGAAAG GAATGGATGT GCTCCATGAC TTTGGAATCC AGTCAACGCA CTACCTCCAG 
GTGAATTACC AGGACTCTCA GGATTGGTTC ATCTTGGTGT CCGTGATTGC AGACCTCAGG 
AATGCCTTCT ATGTCCTCTT TCCCATCTGG TTCCATCTGC GTGAAGCTGT GGGCATCAAA 
CTTCTCTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAATG GATTCTGTTT 
GGACAGCGTC CATACTGGTG GGTCATGGAC ACCGACTACT ATAGCAACAC CTCTGTGCCA 
CTGATAAAGC AATTTCCAGT TACCTGTGAA ACTGGACCAG GGAGTCCCTC TGGTCATGCC 
ATGGGTACAG CAGGTGTATA CTACGTGATG GTCACATCTA CCCTTTCTAT CTTTCGGGGG 
AGAAAAAGGC CAACCTACAG ATTTCGGTGC TTGAATATCC TTCTGTGGTT GGGATTCTGG 
GCTGTGCAGC TGAACGTCTG TCTGTCCCGA ATCTACCTTG CTGCTCATTT CCCCCATCAG 
GTTGTTGCTG GAGTCCTGTC AGGCATTGCT GTTGCTGAAA CTTTCCGCCA CATCCAGAGC 
ATCTACAATG CCAGCCTCAA GAAATATTTT CTCATTACTT TCTTCCTGTT CAGTTTTGCC 
ATTGGATTTT ACCTGCTGCT CAAGGGGCTG GGTGTGGACC TCCTGTGGAC ACTGGAAAAA 
GCCAGGAGAT GGTGTGAGCG GCCGGAATGG GTTCACATTG ACACCACACC CTTTGCCAGC 
CTTCTCAAGA ACGTGGGGAC CCTCTTTGGC CTGGGGGTGG CTCTCAACTC CAGCATGTAC 
AGGGAAAGCT GCAAGGGCAA GCTTAGCAAG TGGTTCCCAT TCCGCCTCAG CTGCATTGTG 
GTGTCTCTCA TCCTCCTGCA CCTCTTTGAC TCTTTGAAAC CCCCATCCCA AACTGAGCTG 
ATCTTCTACA CCTTGTCCTT CTGCAAGAGT GCAGCAGTGC CCCTGGCATC TGTCAGCCTC 
ATCCCCTACT GCCTTGCCCG GGTCTTCGAC CAGCCAGACA AGAAGTCTTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

G6PC ( XM_022422515.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022278223.1 Canis lupus familiaris glucose-6-phosphatase catalytic subunit (G6PC), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe11461
Clone ID Related Accession (Same CDS sequence)	XM_022422515.1 , NM_001002993.2
Accession Version	XM_022422515.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	glucose-6-phosphatase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006591.3) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAAAG GAATGGATGT GCTCCATGAC TTTGGAATCC AGTCAACGCA CTACCTCCAG 
GTGAATTACC AGGACTCTCA GGATTGGTTC ATCTTGGTGT CCGTGATTGC AGACCTCAGG 
AATGCCTTCT ATGTCCTCTT TCCCATCTGG TTCCATCTGC GTGAAGCTGT GGGCATCAAA 
CTTCTCTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAATG GATTCTGTTT 
GGACAGCGTC CATACTGGTG GGTCATGGAC ACCGACTACT ATAGCAACAC CTCTGTGCCA 
CTGATAAAGC AATTTCCAGT TACCTGTGAA ACTGGACCAG GGAGTCCCTC TGGTCATGCC 
ATGGGTACAG CAGGTGTATA CTACGTGATG GTCACATCTA CCCTTTCTAT CTTTCGGGGG 
AGAAAAAGGC CAACCTACAG ATTTCGGTGC TTGAATATCC TTCTGTGGTT GGGATTCTGG 
GCTGTGCAGC TGAACGTCTG TCTGTCCCGA ATCTACCTTG CTGCTCATTT CCCCCATCAG 
GTTGTTGCTG GAGTCCTGTC AGGCATTGCT GTTGCTGAAA CTTTCCGCCA CATCCAGAGC 
ATCTACAATG CCAGCCTCAA GAAATATTTT CTCATTACTT TCTTCCTGTT CAGTTTTGCC 
ATTGGATTTT ACCTGCTGCT CAAGGGGCTG GGTGTGGACC TCCTGTGGAC ACTGGAAAAA 
GCCAGGAGAT GGTGTGAGCG GCCGGAATGG GTTCACATTG ACACCACACC CTTTGCCAGC 
CTTCTCAAGA ACGTGGGGAC CCTCTTTGGC CTGGGGGTGG CTCTCAACTC CAGCATGTAC 
AGGGAAAGCT GCAAGGGCAA GCTTAGCAAG TGGTTCCCAT TCCGCCTCAG CTGCATTGTG 
GTGTCTCTCA TCCTCCTGCA CCTCTTTGAC TCTTTGAAAC CCCCATCCCA AACTGAGCTG 
ATCTTCTACA CCTTGTCCTT CTGCAAGAGT GCAGCAGTGC CCCTGGCATC TGTCAGCCTC 
ATCCCCTACT GCCTTGCCCG GGTCTTCGAC CAGCCAGACA AGAAGTCTTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022278223.1
CDS	66..1139
Translation	MEKGMDVLHDFGIQSTHYLQVNYQDSQDWFILVSVIADLRNAFYVLFPIWFHLREAVGIKLLWVAVIGDWLNLVFKWILFGQRPYWWVMDTDYYSNTSVPLIKQFPVTCETGPGSPSGHAMGTAGVYYVMVTSTLSIFRGRKRPTYRFRCLNILLWLGFWAVQLNVCLSRIYLAAHFPHQVVAGVLSGIAVAETFRHIQSIYNASLKKYFLITFFLFSFAIGFYLLLKGLGVDLLWTLEKARRWCERPEWVHIDTTPFASLLKNVGTLFGLGVALNSSMYRESCKGKLSKWFPFRLSCIVVSLILLHLFDSLKPPSQTELIFYTLSFCKSAAVPLASVSLIPYCLARVFDQPDKKSL

Target ORF information:

RefSeq Version	XM_022422515.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris glucose-6-phosphatase catalytic subunit (G6PC), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022422515.1

ORF Insert Sequence:

ATGGAGAAAG GAATGGATGT GCTCCATGAC TTTGGAATCC AGTCAACGCA CTACCTCCAG 
GTGAATTACC AGGACTCTCA GGATTGGTTC ATCTTGGTGT CCGTGATTGC AGACCTCAGG 
AATGCCTTCT ATGTCCTCTT TCCCATCTGG TTCCATCTGC GTGAAGCTGT GGGCATCAAA 
CTTCTCTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAATG GATTCTGTTT 
GGACAGCGTC CATACTGGTG GGTCATGGAC ACCGACTACT ATAGCAACAC CTCTGTGCCA 
CTGATAAAGC AATTTCCAGT TACCTGTGAA ACTGGACCAG GGAGTCCCTC TGGTCATGCC 
ATGGGTACAG CAGGTGTATA CTACGTGATG GTCACATCTA CCCTTTCTAT CTTTCGGGGG 
AGAAAAAGGC CAACCTACAG ATTTCGGTGC TTGAATATCC TTCTGTGGTT GGGATTCTGG 
GCTGTGCAGC TGAACGTCTG TCTGTCCCGA ATCTACCTTG CTGCTCATTT CCCCCATCAG 
GTTGTTGCTG GAGTCCTGTC AGGCATTGCT GTTGCTGAAA CTTTCCGCCA CATCCAGAGC 
ATCTACAATG CCAGCCTCAA GAAATATTTT CTCATTACTT TCTTCCTGTT CAGTTTTGCC 
ATTGGATTTT ACCTGCTGCT CAAGGGGCTG GGTGTGGACC TCCTGTGGAC ACTGGAAAAA 
GCCAGGAGAT GGTGTGAGCG GCCGGAATGG GTTCACATTG ACACCACACC CTTTGCCAGC 
CTTCTCAAGA ACGTGGGGAC CCTCTTTGGC CTGGGGGTGG CTCTCAACTC CAGCATGTAC 
AGGGAAAGCT GCAAGGGCAA GCTTAGCAAG TGGTTCCCAT TCCGCCTCAG CTGCATTGTG 
GTGTCTCTCA TCCTCCTGCA CCTCTTTGAC TCTTTGAAAC CCCCATCCCA AACTGAGCTG 
ATCTTCTACA CCTTGTCCTT CTGCAAGAGT GCAGCAGTGC CCCTGGCATC TGTCAGCCTC 
ATCCCCTACT GCCTTGCCCG GGTCTTCGAC CAGCCAGACA AGAAGTCTTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selective stimulation of G-6-Pase catalytic subunit but not G-6-P transporter gene expression by glucagon in vivo and cAMP in situ.
American journal of physiology. Endocrinology and metabolism286(5)E795-808(2004 May)
Hornbuckle LA,Everett CA,Martin CC,Gustavson SS,Svitek CA,Oeser JK,Neal DW,Cherrington AD,O'Brien RM

Selective tonic inhibition of G-6-Pase catalytic subunit, but not G-6-P transporter, gene expression by insulin in vivo.
American journal of physiology. Endocrinology and metabolism281(4)E713-25(2001 Oct)
Hornbuckle LA,Edgerton DS,Ayala JE,Svitek CA,Oeser JK,Neal DW,Cardin S,Cherrington AD,O'Brien RM

Isolation and nucleotide sequence of canine glucose-6-phosphatase mRNA: identification of mutation in puppies with glycogen storage disease type Ia.
Biochemical and molecular medicine61(2)168-77(1997 Aug)
Kishnani PS,Bao Y,Wu JY,Brix AE,Lin JL,Chen YT