G6PC cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	G6PC
Entrez Gene ID	2538
Full Name	glucose-6-phosphatase catalytic subunit
Synonyms	G6PC1,G6PT,G6Pase,GSD1,GSD1a
General protein information	Preferred Names glucose-6-phosphatase catalytic subunit Names glucose-6-phosphatase G-6-Pase G6Pase-alpha glucose-6-phosphatase alpha
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q21.31
Summary	Glucose-6-phosphatase (G6Pase) is a multi-subunit integral membrane protein of the endoplasmic reticulum that is composed of a catalytic subunit and transporters for G6P, inorganic phosphate, and glucose. This gene (G6PC) is one of the three glucose-6-phosphatase catalytic-subunit-encoding genes in human: G6PC, G6PC2 and G6PC3. Glucose-6-phosphatase catalyzes the hydrolysis of D-glucose 6-phosphate to D-glucose and orthophosphate and is a key enzyme in glucose homeostasis, functioning in gluconeogenesis and glycogenolysis. Mutations in this gene cause glycogen storage disease type I (GSD1). This disease, also known as von Gierke disease, is a metabolic disorder characterized by severe hypoglycemia associated with the accumulation of glycogen and fat in the liver and kidneys.[provided by RefSeq, Feb 2011].
Disorder MIM:	613742
Disorder Html:	Glycogen storage disease Ia, 232200 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001270397.1	NP_001257326.1	glucose-6-phosphatase isoform 2
NM_000151.3	NP_000142.2	glucose-6-phosphatase isoform 1
NM_000151.4	NP_000142.2	glucose-6-phosphatase isoform 1
NM_001270397.2	NP_001257326.1	glucose-6-phosphatase isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	G6PC	XP_003315515.1
Danio rerio (zebrafish)	g6pca.2	NP_001157278.1
Homo sapiens (human)	G6PC	NP_000142.2
Xenopus tropicalis (tropical clawed frog)	g6pc2	NP_001096531.1
	G6PC	XP_001112333.1
Gallus gallus (chicken)	LOC100857298	XP_003642865.1
Canis lupus familiaris (dog)	G6PC	NP_001002993.1
Xenopus tropicalis (tropical clawed frog)	g6pc	NP_001005682.1
Bos taurus (cattle)	G6PC	NP_001069592.1
Mus musculus (house mouse)	G6pc	NP_032087.2
Rattus norvegicus (Norway rat)	G6pc	NP_037230.2

Gene Ontology
Bibliography

Related articles in PubMed

Glucose-6-phosphatase-? participates in dopaminergic differentiation.
Chen J, Wang T, Zhou XY, Tang CX, Gao DS
Neurological research39(10)869-876(2017 Oct)

3'-UTR SNP rs2229611 in G6PC1 affects mRNA stability, expression and Glycogen Storage Disease type-Ia risk.
Karthi S, Rajeshwari M, Francis A, Saravanan M, Varalakshmi P, Houlden H, Thangaraj K, Ashokkumar B
Clinica chimica acta; international journal of clinical chemistry47146-54(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

crystal structures of the FoxO1 DNA binding domain in complex with the G6PC1 promoter
Title: Crystal structures reveal a new and novel FoxO1 binding site within the human glucose-6-phosphatase catalytic subunit 1 gene promoter.

Mutation analysis of the G6PC gene revealed that GSD Ia accounted for 11% in GSD patients with involvement of liver. Three patients were homozygous for R83C mutation. In addition, a novel stop mutation, Y85X, was identified in a patient with the typical features of GSD Ia.
Title: Molecular analysis of glycogen storage disease type Ia in ian Azeri Turks: identification of a novel mutation.

Notch1 expression is reduced and glucose-6-phosphatase and perilipin-5 (G6PC/PLIN5) are upregulated in liver biopsies from nonalcoholic steatohepatitis (NASH) and nonalcoholic fatty liver disease (NAFLD) patients.
Title: Hepatic Notch1 deletion predisposes to diabetes and steatosis via glucose-6-phosphatase and perilipin-5 upregulation.

Post-translational regulation of the glucose-6-phosphatase complex by cyclic AMP is a crucial determinant of endogenous glucose production and is controlled by the glucose-6-phosphate transporter.
Title: Post-Translational Regulation of the Glucose-6-Phosphatase Complex by Cyclic Adenosine Monophosphate Is a Crucial Determinant of Endogenous Glucose Production and Is Controlled by the Glucose-6-Phosphate Transporter.

ApoA-IV colocalizes with NR4A1, which suppresses G6Pase and PEPCK gene expression at the transcriptional level, reducing hepatic glucose output and lowering blood glucose.
Title: Interaction of ApoA-IV with NR4A1 and NR1D1 Represses G6Pase and PEPCK Transcription: Nuclear Receptor-Mediated Downregulation of Hepatic Gluconeogenesis in Mice and a Human Hepatocyte Cell Line.

The following G6PC gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the G6PC cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20803	NM_000151.4 Latest version!	Homo sapiens glucose-6-phosphatase catalytic subunit (G6PC), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu21715	NM_001270397.2 Latest version!	Homo sapiens glucose-6-phosphatase catalytic subunit (G6PC), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu20803	NM_000151.3	Homo sapiens glucose-6-phosphatase catalytic subunit (G6PC), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu21715	NM_001270397.1	Homo sapiens glucose-6-phosphatase catalytic subunit (G6PC), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

G6PC ( NM_000151.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000142.2 Homo sapiens glucose-6-phosphatase catalytic subunit (G6PC), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20803
Clone ID Related Accession (Same CDS sequence)	NM_000151.3 , NM_000151.4
Accession Version	NM_000151.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	glucose-6-phosphatase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK303771.1, U01120.1, AC016889.28 and AW614228.1. On Jul 3, 2012 this sequence version replaced NM_000151.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U01120.1, BC130478.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG 
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA 
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT 
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC 
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAGCCCCTC TGGCCATGCC 
ATGGGCACAG CAGGTGTATA CTACGTGATG GTCACATCTA CTCTTTCCAT CTTTCAGGGA 
AAGATAAAGC CGACCTACAG ATTTCGGTGC TTGAATGTCA TTTTGTGGTT GGGATTCTGG 
GCTGTGCAGC TGAATGTCTG TCTGTCACGA ATCTACCTTG CTGCTCATTT TCCTCATCAA 
GTTGTTGCTG GAGTCCTGTC AGGCATTGCT GTTGCAGAAA CTTTCAGCCA CATCCACAGC 
ATCTATAATG CCAGCCTCAA GAAATATTTT CTCATTACCT TCTTCCTGTT CAGCTTCGCC 
ATCGGATTTT ATCTGCTGCT CAAGGGACTG GGTGTAGACC TCCTGTGGAC TCTGGAGAAA 
GCCCAGAGGT GGTGCGAGCA GCCAGAATGG GTCCACATTG ACACCACACC CTTTGCCAGC 
CTCCTCAAGA ACCTGGGCAC GCTCTTTGGC CTGGGGCTGG CTCTCAACTC CAGCATGTAC 
AGGGAGAGCT GCAAGGGGAA ACTCAGCAAG TGGCTCCCAT TCCGCCTCAG CTCTATTGTA 
GCCTCCCTCG TCCTCCTGCA CGTCTTTGAC TCCTTGAAAC CCCCATCCCA AGTCGAGCTG 
GTCTTCTACG TCTTGTCCTT CTGCAAGAGT GCGGTAGTGC CCCTGGCATC CGTCAGTGTC 
ATCCCCTACT GCCTCGCCCA GGTCCTGGGC CAGCCGCACA AGAAGTCGTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000142.2
CDS	81..1154
Translation	MEEGMNVLHDFGIQSTHYLQVNYQDSQDWFILVSVIADLRNAFYVLFPIWFHLQEAVGIKLLWVAVIGDWLNLVFKWILFGQRPYWWVLDTDYYSNTSVPLIKQFPVTCETGPGSPSGHAMGTAGVYYVMVTSTLSIFQGKIKPTYRFRCLNVILWLGFWAVQLNVCLSRIYLAAHFPHQVVAGVLSGIAVAETFSHIHSIYNASLKKYFLITFFLFSFAIGFYLLLKGLGVDLLWTLEKAQRWCEQPEWVHIDTTPFASLLKNLGTLFGLGLALNSSMYRESCKGKLSKWLPFRLSSIVASLVLLHVFDSLKPPSQVELVFYVLSFCKSAVVPLASVSVIPYCLAQVLGQPHKKSL

Target ORF information:

RefSeq Version	NM_000151.3
Organism	Homo sapiens(human)
Definition	Homo sapiens glucose-6-phosphatase catalytic subunit (G6PC), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000151.3

ORF Insert Sequence:

ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG 
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA 
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT 
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC 
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAGCCCCTC TGGCCATGCC 
ATGGGCACAG CAGGTGTATA CTACGTGATG GTCACATCTA CTCTTTCCAT CTTTCAGGGA 
AAGATAAAGC CGACCTACAG ATTTCGGTGC TTGAATGTCA TTTTGTGGTT GGGATTCTGG 
GCTGTGCAGC TGAATGTCTG TCTGTCACGA ATCTACCTTG CTGCTCATTT TCCTCATCAA 
GTTGTTGCTG GAGTCCTGTC AGGCATTGCT GTTGCAGAAA CTTTCAGCCA CATCCACAGC 
ATCTATAATG CCAGCCTCAA GAAATATTTT CTCATTACCT TCTTCCTGTT CAGCTTCGCC 
ATCGGATTTT ATCTGCTGCT CAAGGGACTG GGTGTAGACC TCCTGTGGAC TCTGGAGAAA 
GCCCAGAGGT GGTGCGAGCA GCCAGAATGG GTCCACATTG ACACCACACC CTTTGCCAGC 
CTCCTCAAGA ACCTGGGCAC GCTCTTTGGC CTGGGGCTGG CTCTCAACTC CAGCATGTAC 
AGGGAGAGCT GCAAGGGGAA ACTCAGCAAG TGGCTCCCAT TCCGCCTCAG CTCTATTGTA 
GCCTCCCTCG TCCTCCTGCA CGTCTTTGAC TCCTTGAAAC CCCCATCCCA AGTCGAGCTG 
GTCTTCTACG TCTTGTCCTT CTGCAAGAGT GCGGTAGTGC CCCTGGCATC CGTCAGTGTC 
ATCCCCTACT GCCTCGCCCA GGTCCTGGGC CAGCCGCACA AGAAGTCGTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

G6PC ( NM_000151.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000142.2 Homo sapiens glucose-6-phosphatase catalytic subunit (G6PC), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20803
Clone ID Related Accession (Same CDS sequence)	NM_000151.3 , NM_000151.4
Accession Version	NM_000151.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1074bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	glucose-6-phosphatase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U01120.1, AC016889.28 and AW614228.1. On May 17, 2019 this sequence version replaced NM_000151.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U01120.1, SRR5189664.10415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2144835 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved MANE Ensembl match :: ENST00000253801.7/ ENSP00000253801.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG 
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA 
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT 
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC 
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAGCCCCTC TGGCCATGCC 
ATGGGCACAG CAGGTGTATA CTACGTGATG GTCACATCTA CTCTTTCCAT CTTTCAGGGA 
AAGATAAAGC CGACCTACAG ATTTCGGTGC TTGAATGTCA TTTTGTGGTT GGGATTCTGG 
GCTGTGCAGC TGAATGTCTG TCTGTCACGA ATCTACCTTG CTGCTCATTT TCCTCATCAA 
GTTGTTGCTG GAGTCCTGTC AGGCATTGCT GTTGCAGAAA CTTTCAGCCA CATCCACAGC 
ATCTATAATG CCAGCCTCAA GAAATATTTT CTCATTACCT TCTTCCTGTT CAGCTTCGCC 
ATCGGATTTT ATCTGCTGCT CAAGGGACTG GGTGTAGACC TCCTGTGGAC TCTGGAGAAA 
GCCCAGAGGT GGTGCGAGCA GCCAGAATGG GTCCACATTG ACACCACACC CTTTGCCAGC 
CTCCTCAAGA ACCTGGGCAC GCTCTTTGGC CTGGGGCTGG CTCTCAACTC CAGCATGTAC 
AGGGAGAGCT GCAAGGGGAA ACTCAGCAAG TGGCTCCCAT TCCGCCTCAG CTCTATTGTA 
GCCTCCCTCG TCCTCCTGCA CGTCTTTGAC TCCTTGAAAC CCCCATCCCA AGTCGAGCTG 
GTCTTCTACG TCTTGTCCTT CTGCAAGAGT GCGGTAGTGC CCCTGGCATC CGTCAGTGTC 
ATCCCCTACT GCCTCGCCCA GGTCCTGGGC CAGCCGCACA AGAAGTCGTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000142.2
CDS	79..1152
Translation	MEEGMNVLHDFGIQSTHYLQVNYQDSQDWFILVSVIADLRNAFYVLFPIWFHLQEAVGIKLLWVAVIGDWLNLVFKWILFGQRPYWWVLDTDYYSNTSVPLIKQFPVTCETGPGSPSGHAMGTAGVYYVMVTSTLSIFQGKIKPTYRFRCLNVILWLGFWAVQLNVCLSRIYLAAHFPHQVVAGVLSGIAVAETFSHIHSIYNASLKKYFLITFFLFSFAIGFYLLLKGLGVDLLWTLEKAQRWCEQPEWVHIDTTPFASLLKNLGTLFGLGLALNSSMYRESCKGKLSKWLPFRLSSIVASLVLLHVFDSLKPPSQVELVFYVLSFCKSAVVPLASVSVIPYCLAQVLGQPHKKSL

Target ORF information:

RefSeq Version	NM_000151.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glucose-6-phosphatase catalytic subunit (G6PC), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000151.4

ORF Insert Sequence:

ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG 
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA 
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT 
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC 
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAGCCCCTC TGGCCATGCC 
ATGGGCACAG CAGGTGTATA CTACGTGATG GTCACATCTA CTCTTTCCAT CTTTCAGGGA 
AAGATAAAGC CGACCTACAG ATTTCGGTGC TTGAATGTCA TTTTGTGGTT GGGATTCTGG 
GCTGTGCAGC TGAATGTCTG TCTGTCACGA ATCTACCTTG CTGCTCATTT TCCTCATCAA 
GTTGTTGCTG GAGTCCTGTC AGGCATTGCT GTTGCAGAAA CTTTCAGCCA CATCCACAGC 
ATCTATAATG CCAGCCTCAA GAAATATTTT CTCATTACCT TCTTCCTGTT CAGCTTCGCC 
ATCGGATTTT ATCTGCTGCT CAAGGGACTG GGTGTAGACC TCCTGTGGAC TCTGGAGAAA 
GCCCAGAGGT GGTGCGAGCA GCCAGAATGG GTCCACATTG ACACCACACC CTTTGCCAGC 
CTCCTCAAGA ACCTGGGCAC GCTCTTTGGC CTGGGGCTGG CTCTCAACTC CAGCATGTAC 
AGGGAGAGCT GCAAGGGGAA ACTCAGCAAG TGGCTCCCAT TCCGCCTCAG CTCTATTGTA 
GCCTCCCTCG TCCTCCTGCA CGTCTTTGAC TCCTTGAAAC CCCCATCCCA AGTCGAGCTG 
GTCTTCTACG TCTTGTCCTT CTGCAAGAGT GCGGTAGTGC CCCTGGCATC CGTCAGTGTC 
ATCCCCTACT GCCTCGCCCA GGTCCTGGGC CAGCCGCACA AGAAGTCGTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

G6PC ( NM_001270397.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257326.1 Homo sapiens glucose-6-phosphatase catalytic subunit (G6PC), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21715
Clone ID Related Accession (Same CDS sequence)	NM_001270397.1 , NM_001270397.2
Accession Version	NM_001270397.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	glucose-6-phosphatase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK303771.1, BC130478.1, AC016889.28 and AW614228.1. Transcript Variant: This variant (2) lacks an internal segment in the coding region, which results in a frameshift, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303771.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG 
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA 
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT 
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC 
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAAAGATAA AGCCGACCTA 
CAGATTTCGG TGCTTGAATG TCATTTTGTG GTTGGGATTC TGGGCTGTGC AGCTGAATGT 
CTGTCTGTCA CGAATCTACC TTGCTGCTCA TTTTCCTCAT CAAGTTGTTG CTGGAGTCCT 
GTCAGGCATT GCTGTTGCAG AAACTTTCAG CCACATCCAC AGCATCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257326.1
CDS	81..611
Translation	MEEGMNVLHDFGIQSTHYLQVNYQDSQDWFILVSVIADLRNAFYVLFPIWFHLQEAVGIKLLWVAVIGDWLNLVFKWILFGQRPYWWVLDTDYYSNTSVPLIKQFPVTCETGPGKDKADLQISVLECHFVVGILGCAAECLSVTNLPCCSFSSSSCCWSPVRHCCCRNFQPHPQHL

Target ORF information:

RefSeq Version	NM_001270397.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glucose-6-phosphatase catalytic subunit (G6PC), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270397.1

ORF Insert Sequence:

ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG 
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA 
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT 
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC 
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAAAGATAA AGCCGACCTA 
CAGATTTCGG TGCTTGAATG TCATTTTGTG GTTGGGATTC TGGGCTGTGC AGCTGAATGT 
CTGTCTGTCA CGAATCTACC TTGCTGCTCA TTTTCCTCAT CAAGTTGTTG CTGGAGTCCT 
GTCAGGCATT GCTGTTGCAG AAACTTTCAG CCACATCCAC AGCATCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

G6PC ( NM_001270397.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001257326.1 Homo sapiens glucose-6-phosphatase catalytic subunit (G6PC), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21715
Clone ID Related Accession (Same CDS sequence)	NM_001270397.1 , NM_001270397.2
Accession Version	NM_001270397.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	glucose-6-phosphatase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK303771.1, BC130478.1, AC016889.28 and AW614228.1. On Jun 1, 2019 this sequence version replaced NM_001270397.1. Transcript Variant: This variant (2) lacks an internal segment in the coding region, which results in a frameshift, compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK303771.1, DRR138515.97107.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145122 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved ##RefSeq-Attributes-END##

ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG 
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA 
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT 
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC 
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAAAGATAA AGCCGACCTA 
CAGATTTCGG TGCTTGAATG TCATTTTGTG GTTGGGATTC TGGGCTGTGC AGCTGAATGT 
CTGTCTGTCA CGAATCTACC TTGCTGCTCA TTTTCCTCAT CAAGTTGTTG CTGGAGTCCT 
GTCAGGCATT GCTGTTGCAG AAACTTTCAG CCACATCCAC AGCATCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001257326.1
CDS	79..609
Translation	MEEGMNVLHDFGIQSTHYLQVNYQDSQDWFILVSVIADLRNAFYVLFPIWFHLQEAVGIKLLWVAVIGDWLNLVFKWILFGQRPYWWVLDTDYYSNTSVPLIKQFPVTCETGPGKDKADLQISVLECHFVVGILGCAAECLSVTNLPCCSFSSSSCCWSPVRHCCCRNFQPHPQHL

Target ORF information:

RefSeq Version	NM_001270397.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glucose-6-phosphatase catalytic subunit (G6PC), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001270397.2

ORF Insert Sequence:

ATGGAGGAAG GAATGAATGT TCTCCATGAC TTTGGGATCC AGTCAACACA TTACCTCCAG 
GTGAATTACC AAGACTCCCA GGACTGGTTC ATCTTGGTGT CCGTGATCGC AGACCTCAGG 
AATGCCTTCT ACGTCCTCTT CCCCATCTGG TTCCATCTTC AGGAAGCTGT GGGCATTAAA 
CTCCTTTGGG TAGCTGTGAT TGGAGACTGG CTCAACCTCG TCTTTAAGTG GATTCTCTTT 
GGACAGCGTC CATACTGGTG GGTTTTGGAT ACTGACTACT ACAGCAACAC TTCCGTGCCC 
CTGATAAAGC AGTTCCCTGT AACCTGTGAG ACTGGACCAG GGAAAGATAA AGCCGACCTA 
CAGATTTCGG TGCTTGAATG TCATTTTGTG GTTGGGATTC TGGGCTGTGC AGCTGAATGT 
CTGTCTGTCA CGAATCTACC TTGCTGCTCA TTTTCCTCAT CAAGTTGTTG CTGGAGTCCT 
GTCAGGCATT GCTGTTGCAG AAACTTTCAG CCACATCCAC AGCATCTATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Glucose-6-phosphatase-? participates in dopaminergic differentiation.
Neurological research39(10)869-876(2017 Oct)
Chen J,Wang T,Zhou XY,Tang CX,Gao DS

3'-UTR SNP rs2229611 in G6PC1 affects mRNA stability, expression and Glycogen Storage Disease type-Ia risk.
Clinica chimica acta; international journal of clinical chemistry47146-54(2017 Aug)
Karthi S,Rajeshwari M,Francis A,Saravanan M,Varalakshmi P,Houlden H,Thangaraj K,Ashokkumar B

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G6PC cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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