MIA3 cDNA ORF clone, Canis lupus familiaris(dog)

The following MIA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe285057 XM_022415456.1
Latest version!
Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe45302 XM_014111164.2
Latest version!
Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe285056 XM_022415455.1
Latest version!
Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe31714 XM_005640835.3
Latest version!
Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe31715 XM_005640836.3
Latest version!
Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe31716 XM_005640837.3
Latest version!
Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe45301 XM_014111163.1
Latest version!
Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
View Old Accession Versions >>
OCe45302 XM_014111164.1 Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $426.30
$609.00
OCe31716 XM_005640837.2 Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe31714 XM_005640835.2 Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
OCe31715 XM_005640836.2 Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1021.30
$1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe285057
    Clone ID Related Accession (Same CDS sequence) XM_022415456.1
    Accession Version XM_022415456.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2430bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product transport and Golgi organization protein 1 homolog isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGACTCCG TTCCCGCCAC TGTGCCTTCT GTCACCGCCT CCCCGGGGGA CTCGGAGCTC 
    CTGGGACCCC TGTCGGTGCT CTACGCGGCC CTCATAGCCA AGCTGCTGGA GTTAGTTGCT
    ACATTGCCTG ATGACGTTCA GCCTGGGCCT GATTTTTATG GCTTGCCATG GAAGCCTGTA
    CTTCTCACTG CCTTCTTGGG AATTGGTTCA TTTGCGGTCT TCTTCTGGAA AACTGTCCTT
    GTTGTAAAGA GTAGAGTCTA TCAAGTCACT GAACAACAAA TTTCTGAGAA GTTGAAGAAC
    ATCATGAAAG AAAATGCAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATTAAG
    GAATCAAAAA AACATGTTCA AGAAACCAAG AAACAAAATA TAATTCTGTC TGATGAAGCA
    ATTAAATTTA AGGATAAAAT CAAGAAACTT GAAGAAACAA ATGAAATTCT GGACGATACA
    GCTAAAAATC TGCGTGTTAT GTTAGAATCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC
    TTGATACTGG AAAACAAGAA ATCTATAGAG AAGCTGAAGG ATGTTATTTC AGTGAATGCC
    TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAGGTG
    AAGTCTGAAT GCCATCGAGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA GAAGGAACAG
    TTGCAGCAAG AAATCAAAGA TTGGAGCAAA TCACACGCTG AGCTCAGTGA GCAAATCAAA
    TCATTTGAGA CATCTCAGAA AAATTTGGAA GTAGCTCTTA CTCACAAAGA TGATAATATT
    AATGCTTTGA CTAATTGCAT TACCCAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG
    GATCAGAGTA AAGGAGGAAG TGAGTTAGAT GAGTTAGCAA ATGGAGAAGT AGGAGGTGAC
    CGGAGTGAGA AAATGAAAAA TCAGATTAAG CAGATGATGG ACATTTCTCG GACACAAACT
    GCAATATCAG TAGTTGAAGA GGATCTAAAA CTTCTGCAGA TTAAGCTTAG AGCCTCAATG
    TCTACTAAAT GCAACCTGGA AGACCAAATA AAGAAATTAG AAGATGACCG TGATTCACTG
    CAGTCTGCCA AAGCCGGCCT GGAGGACGAA TGCAAAACCC TGAGGCAAAA AGTGGAGATT
    CTGAACGAAC TCTATCAACA GAAGGAAATG GCTCTGCAAA AGAAACTGAG TCAGGAAGAG
    TATGAGCGGC AGGAGAGAGA GCAGCGGCTG TCAGCTGCAG ACGAGAAGGC GCTTCTGGCT
    GCGGAGGAAG TAAAAACGTA CAAGCGTAGA ATCGAAGAGA TGGAGGATGA GTTGCAGAAG
    ACAGAGCGCT CGTTTAAAAA CCAGATTGCG ACTCATGAGA AGAAAGCTCA TGATAACTGG
    CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCCGAGGAGA AGAGGGAAGC TGCCAACTTG
    AGACACAAGT TACTGGAATT AACCCAGAAG ATGGCAATGC TGCAAGAGGA GCCTGTGATT
    GTGAAGCCGA GGCCGGGCCG ACCCAGCGCC CAGAACCCTC CGTGGAGAGG TCCTTTGAGC
    CAGAACGGCT CTTTTGGTCC ATCCCCTGTG AGTGGGGGGG AGTGCTCCCC ACCTCCCACG
    GCTGACCTGC CTGCGAGACC GCTCTCTGCT ACGCTGAATC GAAGAGAGAT GCCTAGAGGT
    GAATTTGGGT CAGTGGACGG GCCTCTACCT CGTCCTCGTT GGTCATCTGA GGCATCTGGG
    AAACCCTCTG CCTCTGATCT GGGATCTGGT GCAGCTCCCA CGATGAACAG CAGCTCGAGA
    AGCTCTTCCC CTACCAAGGT GACGGATGAA GGCAAGCAAA CTGTTCCCCA AGACCCCGAA
    GGCCCTTCAG TTCCCAGCAG TTCACCTGTG GCTGAGCAGT CGGTAGTAGT TCCTATGGCT
    GCAAAAGGGC CGCCTCCTTT CCCAGGGGTG CCCCTCATGG GCTCCCCCGT GGGCGGCCCT
    CTACCACCAC CCATTCGCTA CGGACCACCC CCTCAACTTT GCGGGCCTTT CGGGCCTCGG
    CCGCTTCCTC CACCATTTGG TCCTGCTTTA CGTCCACCAC TAGGCTTAAG AGAATACGCA
    CCAGGCATTC CACCTGGAAA ACGGGACCTG TCTCTTGATC CTCGAGAATT TTTACCGGGA
    CATGCACCTT TTAGACCTTT AGGCTCTTTT GGCCCAAGAG AGTACTTTAT TCCCGGTACC
    CGACTACCAC CCCCTGCCCA TGGTCCCCAG GATTATCCAC CTCCACCTGC TGCAAGAGAC
    TTACTGCCTT CTGGCTCCAG AGACGAGCCT CCACCTGCCT CCCAGGGCAG TAGCCAGGAC
    TGTTCACAGG CTTTAAAACA GAGCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271164.1
    CDS165..2594
    Translation

    Target ORF information:

    RefSeq Version XM_022415456.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022415456.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    ATGGACTCCG TTCCCGCCAC TGTGCCTTCT GTCACCGCCT CCCCGGGGGA CTCGGAGCTC 
    CTGGGACCCC TGTCGGTGCT CTACGCGGCC CTCATAGCCA AGCTGCTGGA GTTAGTTGCT
    ACATTGCCTG ATGACGTTCA GCCTGGGCCT GATTTTTATG GCTTGCCATG GAAGCCTGTA
    CTTCTCACTG CCTTCTTGGG AATTGGTTCA TTTGCGGTCT TCTTCTGGAA AACTGTCCTT
    GTTGTAAAGA GTAGAGTCTA TCAAGTCACT GAACAACAAA TTTCTGAGAA GTTGAAGAAC
    ATCATGAAAG AAAATGCAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATTAAG
    GAATCAAAAA AACATGTTCA AGAAACCAAG AAACAAAATA TAATTCTGTC TGATGAAGCA
    ATTAAATTTA AGGATAAAAT CAAGAAACTT GAAGAAACAA ATGAAATTCT GGACGATACA
    GCTAAAAATC TGCGTGTTAT GTTAGAATCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC
    TTGATACTGG AAAACAAGAA ATCTATAGAG AAGCTGAAGG ATGTTATTTC AGTGAATGCC
    TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAGGTG
    AAGTCTGAAT GCCATCGAGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA GAAGGAACAG
    TTGCAGCAAG AAATCAAAGA TTGGAGCAAA TCACACGCTG AGCTCAGTGA GCAAATCAAA
    TCATTTGAGA CATCTCAGAA AAATTTGGAA GTAGCTCTTA CTCACAAAGA TGATAATATT
    AATGCTTTGA CTAATTGCAT TACCCAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG
    GATCAGAGTA AAGGAGGAAG TGAGTTAGAT GAGTTAGCAA ATGGAGAAGT AGGAGGTGAC
    CGGAGTGAGA AAATGAAAAA TCAGATTAAG CAGATGATGG ACATTTCTCG GACACAAACT
    GCAATATCAG TAGTTGAAGA GGATCTAAAA CTTCTGCAGA TTAAGCTTAG AGCCTCAATG
    TCTACTAAAT GCAACCTGGA AGACCAAATA AAGAAATTAG AAGATGACCG TGATTCACTG
    CAGTCTGCCA AAGCCGGCCT GGAGGACGAA TGCAAAACCC TGAGGCAAAA AGTGGAGATT
    CTGAACGAAC TCTATCAACA GAAGGAAATG GCTCTGCAAA AGAAACTGAG TCAGGAAGAG
    TATGAGCGGC AGGAGAGAGA GCAGCGGCTG TCAGCTGCAG ACGAGAAGGC GCTTCTGGCT
    GCGGAGGAAG TAAAAACGTA CAAGCGTAGA ATCGAAGAGA TGGAGGATGA GTTGCAGAAG
    ACAGAGCGCT CGTTTAAAAA CCAGATTGCG ACTCATGAGA AGAAAGCTCA TGATAACTGG
    CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCCGAGGAGA AGAGGGAAGC TGCCAACTTG
    AGACACAAGT TACTGGAATT AACCCAGAAG ATGGCAATGC TGCAAGAGGA GCCTGTGATT
    GTGAAGCCGA GGCCGGGCCG ACCCAGCGCC CAGAACCCTC CGTGGAGAGG TCCTTTGAGC
    CAGAACGGCT CTTTTGGTCC ATCCCCTGTG AGTGGGGGGG AGTGCTCCCC ACCTCCCACG
    GCTGACCTGC CTGCGAGACC GCTCTCTGCT ACGCTGAATC GAAGAGAGAT GCCTAGAGGT
    GAATTTGGGT CAGTGGACGG GCCTCTACCT CGTCCTCGTT GGTCATCTGA GGCATCTGGG
    AAACCCTCTG CCTCTGATCT GGGATCTGGT GCAGCTCCCA CGATGAACAG CAGCTCGAGA
    AGCTCTTCCC CTACCAAGGT GACGGATGAA GGCAAGCAAA CTGTTCCCCA AGACCCCGAA
    GGCCCTTCAG TTCCCAGCAG TTCACCTGTG GCTGAGCAGT CGGTAGTAGT TCCTATGGCT
    GCAAAAGGGC CGCCTCCTTT CCCAGGGGTG CCCCTCATGG GCTCCCCCGT GGGCGGCCCT
    CTACCACCAC CCATTCGCTA CGGACCACCC CCTCAACTTT GCGGGCCTTT CGGGCCTCGG
    CCGCTTCCTC CACCATTTGG TCCTGCTTTA CGTCCACCAC TAGGCTTAAG AGAATACGCA
    CCAGGCATTC CACCTGGAAA ACGGGACCTG TCTCTTGATC CTCGAGAATT TTTACCGGGA
    CATGCACCTT TTAGACCTTT AGGCTCTTTT GGCCCAAGAG AGTACTTTAT TCCCGGTACC
    CGACTACCAC CCCCTGCCCA TGGTCCCCAG GATTATCCAC CTCCACCTGC TGCAAGAGAC
    TTACTGCCTT CTGGCTCCAG AGACGAGCCT CCACCTGCCT CCCAGGGCAG TAGCCAGGAC
    TGTTCACAGG CTTTAAAACA GAGCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45302
    Clone ID Related Accession (Same CDS sequence) XM_014111164.2 , XM_014111164.1
    Accession Version XM_014111164.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product transport and Golgi organization protein 1 homolog isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111164.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGACTCCG TTCCCGCCAC TGTGCCTTCT GTCACCGCCT CCCCGGGGGA CTCGGAGCTC 
    CTGGGACCCC TGTCGGTGCT CTACGCGGCC CTCATAGCCA AGCTGCTGGA GTTAGTTGCT
    ACATTGCCTG ATGACGTTCA GCCTGGGCCT GATTTTTATG GCTTGCCATG GAAGCCTGTA
    CTTCTCACTG CCTTCTTGGG AATTGGTTCA TTTGCGGTCT TCTTCTGGAA AACTGTCCTT
    GTTGTAAAGA GTAGAGTCTA TCAAGTCACT GAACAACAAA TTTCTGAGAA GTTGAAGAAC
    ATCATGAAAG AAAATGCAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATTAAG
    GAATCAAAAA AACATGTTCA AGAAACCAAG AAACAAAATA TAATTCTGTC TGATGAAGCA
    ATTAAATTTA AGGATAAAAT CAAGAAACTT GAAGAAACAA ATGAAATTCT GGACGATACA
    GCTAAAAATC TGCGTGTTAT GTTAGAATCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC
    TTGATACTGG AAAACAAGAA ATCTATAGAG AAGCTGAAGG ATGTTATTTC AGTGAATGCC
    TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAGGTG
    AAGTCTGAAT GCCATCGAGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA GAAGGAACAG
    TTGCAGCAAG AAATCAAAGA TTGGAGCAAA TCACACGCTG AGCTCAGTGA GCAAATCAAA
    TCATTTGAGA CATCTCAGAA AAATTTGGAA GTAGCTCTTA CTCACAAAGA TGATAATATT
    AATGCTTTGA CTAATTGCAT TACCCAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG
    GATCAGAGTA AAGGAGGAAG TGAGTTAGAT GAGTTAGCAA ATGGAGAAGT AGGAGGTGAC
    CGGAGTGAGA AAATGAAAAA TCAGATTAAG CAGATGATGG ACATTTCTCG GACACAAACT
    GCAATATCAG TAGTTGAAGA GGATCTAAAA CTTCTGCAGA TTAAGCTTAG AGCCTCAATG
    TCTACTAAAT GCAACCTGGA AGACCAAATA AAGAAATTAG AAGATGACCG TGATTCACTG
    CAGTCTGCCA AAGCCGGCCT GGAGGACGAA TGCAAAACCC TGAGGCAAAA AGTGGAGATT
    CTGAACGAAC TCTATCAACA GAAGGAAATG GCTCTGCAAA AGAAACTGAG TCAGGAAGAG
    TATGAGCGGC AGGAGAGAGA GCAGCGGCTG TCAGCTGCAG ACGAGAAGGC GCTTCTGGCT
    GCGGAGGAAG TAAAAACGTA CAAGCGTAGA ATCGAAGAGA TGGAGGATGA GTTGCAGAAG
    ACAGAGCGCT CGTTTAAAAA CCAGATTGCG ACTCATGAGA AGAAAGCTCA TGATAACTGG
    CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCCGAGGAGA AGAGGGAAGC TGCCAACTTG
    AGACACAAGT TACTGGAATT AACCCAGAAG ATGGCAATGC TGCAAGAGGA GCCTGTGATT
    GTGAAGCCGA GGCCGGGCCG ACCCAGCGCC CAGAACCCTC CGTGGAGAGG TCCTTTGAGC
    CAGAACGGCT CTTTTGGTCC ATCCCCTGTG AGTGGGGGGG AGTGCTCCCC ACCTCCCACG
    GCTGACCTGC CTGCGAGACC GCTCTCTGCT ACGCTGAATC GAAGAGAGAT GCCTAGAGGT
    GAATTTGGGT CAGTGGACGG GCCTCTACCT CGTCCTCGTT GGTCATCTGA GGCATCTGGG
    AAACCCTCTG CCTCTGATCT GGGATCTGGT GCAGCTCCCA CGATGAACAG CAGCTCGAGA
    AGCTCTTCCC CTACCAAGGT GACGGATGAA GGCAAGGTTC CTATGGCTGC AAAAGGGCCG
    CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC
    ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA
    CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA
    CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT
    AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC
    CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT
    GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT
    TTAAAACAGA GCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966639.1
    CDS1..2358
    Translation

    Target ORF information:

    RefSeq Version XM_014111164.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111164.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGACTCCG TTCCCGCCAC TGTGCCTTCT GTCACCGCCT CCCCGGGGGA CTCGGAGCTC 
    CTGGGACCCC TGTCGGTGCT CTACGCGGCC CTCATAGCCA AGCTGCTGGA GTTAGTTGCT
    ACATTGCCTG ATGACGTTCA GCCTGGGCCT GATTTTTATG GCTTGCCATG GAAGCCTGTA
    CTTCTCACTG CCTTCTTGGG AATTGGTTCA TTTGCGGTCT TCTTCTGGAA AACTGTCCTT
    GTTGTAAAGA GTAGAGTCTA TCAAGTCACT GAACAACAAA TTTCTGAGAA GTTGAAGAAC
    ATCATGAAAG AAAATGCAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATTAAG
    GAATCAAAAA AACATGTTCA AGAAACCAAG AAACAAAATA TAATTCTGTC TGATGAAGCA
    ATTAAATTTA AGGATAAAAT CAAGAAACTT GAAGAAACAA ATGAAATTCT GGACGATACA
    GCTAAAAATC TGCGTGTTAT GTTAGAATCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC
    TTGATACTGG AAAACAAGAA ATCTATAGAG AAGCTGAAGG ATGTTATTTC AGTGAATGCC
    TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAGGTG
    AAGTCTGAAT GCCATCGAGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA GAAGGAACAG
    TTGCAGCAAG AAATCAAAGA TTGGAGCAAA TCACACGCTG AGCTCAGTGA GCAAATCAAA
    TCATTTGAGA CATCTCAGAA AAATTTGGAA GTAGCTCTTA CTCACAAAGA TGATAATATT
    AATGCTTTGA CTAATTGCAT TACCCAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG
    GATCAGAGTA AAGGAGGAAG TGAGTTAGAT GAGTTAGCAA ATGGAGAAGT AGGAGGTGAC
    CGGAGTGAGA AAATGAAAAA TCAGATTAAG CAGATGATGG ACATTTCTCG GACACAAACT
    GCAATATCAG TAGTTGAAGA GGATCTAAAA CTTCTGCAGA TTAAGCTTAG AGCCTCAATG
    TCTACTAAAT GCAACCTGGA AGACCAAATA AAGAAATTAG AAGATGACCG TGATTCACTG
    CAGTCTGCCA AAGCCGGCCT GGAGGACGAA TGCAAAACCC TGAGGCAAAA AGTGGAGATT
    CTGAACGAAC TCTATCAACA GAAGGAAATG GCTCTGCAAA AGAAACTGAG TCAGGAAGAG
    TATGAGCGGC AGGAGAGAGA GCAGCGGCTG TCAGCTGCAG ACGAGAAGGC GCTTCTGGCT
    GCGGAGGAAG TAAAAACGTA CAAGCGTAGA ATCGAAGAGA TGGAGGATGA GTTGCAGAAG
    ACAGAGCGCT CGTTTAAAAA CCAGATTGCG ACTCATGAGA AGAAAGCTCA TGATAACTGG
    CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCCGAGGAGA AGAGGGAAGC TGCCAACTTG
    AGACACAAGT TACTGGAATT AACCCAGAAG ATGGCAATGC TGCAAGAGGA GCCTGTGATT
    GTGAAGCCGA GGCCGGGCCG ACCCAGCGCC CAGAACCCTC CGTGGAGAGG TCCTTTGAGC
    CAGAACGGCT CTTTTGGTCC ATCCCCTGTG AGTGGGGGGG AGTGCTCCCC ACCTCCCACG
    GCTGACCTGC CTGCGAGACC GCTCTCTGCT ACGCTGAATC GAAGAGAGAT GCCTAGAGGT
    GAATTTGGGT CAGTGGACGG GCCTCTACCT CGTCCTCGTT GGTCATCTGA GGCATCTGGG
    AAACCCTCTG CCTCTGATCT GGGATCTGGT GCAGCTCCCA CGATGAACAG CAGCTCGAGA
    AGCTCTTCCC CTACCAAGGT GACGGATGAA GGCAAGGTTC CTATGGCTGC AAAAGGGCCG
    CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC
    ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA
    CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA
    CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT
    AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC
    CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT
    GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT
    TTAAAACAGA GCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45302
    Clone ID Related Accession (Same CDS sequence) XM_014111164.2 , XM_014111164.1
    Accession Version XM_014111164.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product melanoma inhibitory activity protein 3 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGACTCCG TTCCCGCCAC TGTGCCTTCT GTCACCGCCT CCCCGGGGGA CTCGGAGCTC 
    CTGGGACCCC TGTCGGTGCT CTACGCGGCC CTCATAGCCA AGCTGCTGGA GTTAGTTGCT
    ACATTGCCTG ATGACGTTCA GCCTGGGCCT GATTTTTATG GCTTGCCATG GAAGCCTGTA
    CTTCTCACTG CCTTCTTGGG AATTGGTTCA TTTGCGGTCT TCTTCTGGAA AACTGTCCTT
    GTTGTAAAGA GTAGAGTCTA TCAAGTCACT GAACAACAAA TTTCTGAGAA GTTGAAGAAC
    ATCATGAAAG AAAATGCAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATTAAG
    GAATCAAAAA AACATGTTCA AGAAACCAAG AAACAAAATA TAATTCTGTC TGATGAAGCA
    ATTAAATTTA AGGATAAAAT CAAGAAACTT GAAGAAACAA ATGAAATTCT GGACGATACA
    GCTAAAAATC TGCGTGTTAT GTTAGAATCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC
    TTGATACTGG AAAACAAGAA ATCTATAGAG AAGCTGAAGG ATGTTATTTC AGTGAATGCC
    TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAGGTG
    AAGTCTGAAT GCCATCGAGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA GAAGGAACAG
    TTGCAGCAAG AAATCAAAGA TTGGAGCAAA TCACACGCTG AGCTCAGTGA GCAAATCAAA
    TCATTTGAGA CATCTCAGAA AAATTTGGAA GTAGCTCTTA CTCACAAAGA TGATAATATT
    AATGCTTTGA CTAATTGCAT TACCCAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG
    GATCAGAGTA AAGGAGGAAG TGAGTTAGAT GAGTTAGCAA ATGGAGAAGT AGGAGGTGAC
    CGGAGTGAGA AAATGAAAAA TCAGATTAAG CAGATGATGG ACATTTCTCG GACACAAACT
    GCAATATCAG TAGTTGAAGA GGATCTAAAA CTTCTGCAGA TTAAGCTTAG AGCCTCAATG
    TCTACTAAAT GCAACCTGGA AGACCAAATA AAGAAATTAG AAGATGACCG TGATTCACTG
    CAGTCTGCCA AAGCCGGCCT GGAGGACGAA TGCAAAACCC TGAGGCAAAA AGTGGAGATT
    CTGAACGAAC TCTATCAACA GAAGGAAATG GCTCTGCAAA AGAAACTGAG TCAGGAAGAG
    TATGAGCGGC AGGAGAGAGA GCAGCGGCTG TCAGCTGCAG ACGAGAAGGC GCTTCTGGCT
    GCGGAGGAAG TAAAAACGTA CAAGCGTAGA ATCGAAGAGA TGGAGGATGA GTTGCAGAAG
    ACAGAGCGCT CGTTTAAAAA CCAGATTGCG ACTCATGAGA AGAAAGCTCA TGATAACTGG
    CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCCGAGGAGA AGAGGGAAGC TGCCAACTTG
    AGACACAAGT TACTGGAATT AACCCAGAAG ATGGCAATGC TGCAAGAGGA GCCTGTGATT
    GTGAAGCCGA GGCCGGGCCG ACCCAGCGCC CAGAACCCTC CGTGGAGAGG TCCTTTGAGC
    CAGAACGGCT CTTTTGGTCC ATCCCCTGTG AGTGGGGGGG AGTGCTCCCC ACCTCCCACG
    GCTGACCTGC CTGCGAGACC GCTCTCTGCT ACGCTGAATC GAAGAGAGAT GCCTAGAGGT
    GAATTTGGGT CAGTGGACGG GCCTCTACCT CGTCCTCGTT GGTCATCTGA GGCATCTGGG
    AAACCCTCTG CCTCTGATCT GGGATCTGGT GCAGCTCCCA CGATGAACAG CAGCTCGAGA
    AGCTCTTCCC CTACCAAGGT GACGGATGAA GGCAAGGTTC CTATGGCTGC AAAAGGGCCG
    CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC
    ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA
    CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA
    CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT
    AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC
    CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT
    GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT
    TTAAAACAGA GCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966639.1
    CDS167..2524
    Translation

    Target ORF information:

    RefSeq Version XM_014111164.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111164.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGGACTCCG TTCCCGCCAC TGTGCCTTCT GTCACCGCCT CCCCGGGGGA CTCGGAGCTC 
    CTGGGACCCC TGTCGGTGCT CTACGCGGCC CTCATAGCCA AGCTGCTGGA GTTAGTTGCT
    ACATTGCCTG ATGACGTTCA GCCTGGGCCT GATTTTTATG GCTTGCCATG GAAGCCTGTA
    CTTCTCACTG CCTTCTTGGG AATTGGTTCA TTTGCGGTCT TCTTCTGGAA AACTGTCCTT
    GTTGTAAAGA GTAGAGTCTA TCAAGTCACT GAACAACAAA TTTCTGAGAA GTTGAAGAAC
    ATCATGAAAG AAAATGCAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATTAAG
    GAATCAAAAA AACATGTTCA AGAAACCAAG AAACAAAATA TAATTCTGTC TGATGAAGCA
    ATTAAATTTA AGGATAAAAT CAAGAAACTT GAAGAAACAA ATGAAATTCT GGACGATACA
    GCTAAAAATC TGCGTGTTAT GTTAGAATCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC
    TTGATACTGG AAAACAAGAA ATCTATAGAG AAGCTGAAGG ATGTTATTTC AGTGAATGCC
    TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAGGTG
    AAGTCTGAAT GCCATCGAGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA GAAGGAACAG
    TTGCAGCAAG AAATCAAAGA TTGGAGCAAA TCACACGCTG AGCTCAGTGA GCAAATCAAA
    TCATTTGAGA CATCTCAGAA AAATTTGGAA GTAGCTCTTA CTCACAAAGA TGATAATATT
    AATGCTTTGA CTAATTGCAT TACCCAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG
    GATCAGAGTA AAGGAGGAAG TGAGTTAGAT GAGTTAGCAA ATGGAGAAGT AGGAGGTGAC
    CGGAGTGAGA AAATGAAAAA TCAGATTAAG CAGATGATGG ACATTTCTCG GACACAAACT
    GCAATATCAG TAGTTGAAGA GGATCTAAAA CTTCTGCAGA TTAAGCTTAG AGCCTCAATG
    TCTACTAAAT GCAACCTGGA AGACCAAATA AAGAAATTAG AAGATGACCG TGATTCACTG
    CAGTCTGCCA AAGCCGGCCT GGAGGACGAA TGCAAAACCC TGAGGCAAAA AGTGGAGATT
    CTGAACGAAC TCTATCAACA GAAGGAAATG GCTCTGCAAA AGAAACTGAG TCAGGAAGAG
    TATGAGCGGC AGGAGAGAGA GCAGCGGCTG TCAGCTGCAG ACGAGAAGGC GCTTCTGGCT
    GCGGAGGAAG TAAAAACGTA CAAGCGTAGA ATCGAAGAGA TGGAGGATGA GTTGCAGAAG
    ACAGAGCGCT CGTTTAAAAA CCAGATTGCG ACTCATGAGA AGAAAGCTCA TGATAACTGG
    CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCCGAGGAGA AGAGGGAAGC TGCCAACTTG
    AGACACAAGT TACTGGAATT AACCCAGAAG ATGGCAATGC TGCAAGAGGA GCCTGTGATT
    GTGAAGCCGA GGCCGGGCCG ACCCAGCGCC CAGAACCCTC CGTGGAGAGG TCCTTTGAGC
    CAGAACGGCT CTTTTGGTCC ATCCCCTGTG AGTGGGGGGG AGTGCTCCCC ACCTCCCACG
    GCTGACCTGC CTGCGAGACC GCTCTCTGCT ACGCTGAATC GAAGAGAGAT GCCTAGAGGT
    GAATTTGGGT CAGTGGACGG GCCTCTACCT CGTCCTCGTT GGTCATCTGA GGCATCTGGG
    AAACCCTCTG CCTCTGATCT GGGATCTGGT GCAGCTCCCA CGATGAACAG CAGCTCGAGA
    AGCTCTTCCC CTACCAAGGT GACGGATGAA GGCAAGGTTC CTATGGCTGC AAAAGGGCCG
    CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC
    ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA
    CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA
    CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT
    AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC
    CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT
    GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT
    TTAAAACAGA GCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe285056
    Clone ID Related Accession (Same CDS sequence) XM_022415455.1
    Accession Version XM_022415455.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product transport and Golgi organization protein 1 homolog isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
    AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT
    GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA
    GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA
    GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG
    GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG
    ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA
    CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA
    GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA
    GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA
    CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT
    GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA
    GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG
    ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT
    GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA
    GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA
    GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT
    GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA
    AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT
    ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG
    GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG
    AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT
    GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA
    ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC
    GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT
    GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT
    GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT
    GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA
    GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA
    CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA
    GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA
    CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA
    GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA
    GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG
    GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG
    GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA
    CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC
    GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG
    CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC
    AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG
    GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT
    CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT
    CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC
    AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC
    TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC
    CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC
    TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC
    TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG
    CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT
    GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA
    GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA
    GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC
    ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT
    GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC
    AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC
    CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT
    CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA
    GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA
    AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT
    CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT
    GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC
    TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT
    AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA
    AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA
    CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG
    GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG
    CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA
    AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA
    GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC
    CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA
    ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA
    TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT
    AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA
    GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA
    ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA
    GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC
    AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA
    GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC
    TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG
    GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA
    AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG
    TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT
    GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA
    CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG
    CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT
    TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT
    GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGATCTG
    GGATCTGGTG CAGCTCCCAC GATGAACAGC AGCTCGAGAA GCTCTTCCCC TACCAAGGTG
    ACGGATGAAG GCAAGGTTCC TATGGCTGCA AAAGGGCCGC CTCCTTTCCC AGGGGTGCCC
    CTCATGGGCT CCCCCGTGGG CGGCCCTCTA CCACCACCCA TTCGCTACGG ACCACCCCCT
    CAACTTTGCG GGCCTTTCGG GCCTCGGCCG CTTCCTCCAC CATTTGGTCC TGCTTTACGT
    CCACCACTAG GCTTAAGAGA ATACGCACCA GGCATTCCAC CTGGAAAACG GGACCTGTCT
    CTTGATCCTC GAGAATTTTT ACCGGGACAT GCACCTTTTA GACCTTTAGG CTCTTTTGGC
    CCAAGAGAGT ACTTTATTCC CGGTACCCGA CTACCACCCC CTGCCCATGG TCCCCAGGAT
    TATCCACCTC CACCTGCTGC AAGAGACTTA CTGCCTTCTG GCTCCAGAGA CGAGCCTCCA
    CCTGCCTCCC AGGGCAGTAG CCAGGACTGT TCACAGGCTT TAAAACAGAG CCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022271163.1
    CDS105..5861
    Translation

    Target ORF information:

    RefSeq Version XM_022415455.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022415455.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
    AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT
    GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA
    GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA
    GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG
    GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG
    ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA
    CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA
    GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA
    GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA
    CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT
    GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA
    GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG
    ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT
    GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA
    GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA
    GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT
    GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA
    AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT
    ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG
    GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG
    AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT
    GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA
    ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC
    GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT
    GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT
    GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT
    GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA
    GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA
    CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA
    GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA
    CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA
    GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA
    GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG
    GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG
    GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA
    CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC
    GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG
    CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC
    AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG
    GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT
    CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT
    CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC
    AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC
    TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC
    CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC
    TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC
    TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG
    CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT
    GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA
    GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA
    GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC
    ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT
    GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC
    AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC
    CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT
    CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA
    GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA
    AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT
    CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT
    GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC
    TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT
    AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA
    AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA
    CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG
    GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG
    CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA
    AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA
    GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC
    CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA
    ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA
    TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT
    AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA
    GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA
    ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA
    GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC
    AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA
    GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC
    TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG
    GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA
    AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG
    TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT
    GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA
    CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG
    CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT
    TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT
    GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGATCTG
    GGATCTGGTG CAGCTCCCAC GATGAACAGC AGCTCGAGAA GCTCTTCCCC TACCAAGGTG
    ACGGATGAAG GCAAGGTTCC TATGGCTGCA AAAGGGCCGC CTCCTTTCCC AGGGGTGCCC
    CTCATGGGCT CCCCCGTGGG CGGCCCTCTA CCACCACCCA TTCGCTACGG ACCACCCCCT
    CAACTTTGCG GGCCTTTCGG GCCTCGGCCG CTTCCTCCAC CATTTGGTCC TGCTTTACGT
    CCACCACTAG GCTTAAGAGA ATACGCACCA GGCATTCCAC CTGGAAAACG GGACCTGTCT
    CTTGATCCTC GAGAATTTTT ACCGGGACAT GCACCTTTTA GACCTTTAGG CTCTTTTGGC
    CCAAGAGAGT ACTTTATTCC CGGTACCCGA CTACCACCCC CTGCCCATGG TCCCCAGGAT
    TATCCACCTC CACCTGCTGC AAGAGACTTA CTGCCTTCTG GCTCCAGAGA CGAGCCTCCA
    CCTGCCTCCC AGGGCAGTAG CCAGGACTGT TCACAGGCTT TAAAACAGAG CCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31714
    Clone ID Related Accession (Same CDS sequence) XM_005640835.2 , XM_005640835.3
    Accession Version XM_005640835.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product melanoma inhibitory activity protein 3 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
    AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT
    GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA
    GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA
    GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG
    GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG
    ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA
    CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA
    GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA
    GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA
    CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT
    GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA
    GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG
    ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT
    GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA
    GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA
    GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT
    GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA
    AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT
    ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG
    GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG
    AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT
    GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA
    ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC
    GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT
    GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT
    GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT
    GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA
    GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA
    CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA
    GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA
    CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA
    GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA
    GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG
    GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG
    GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA
    CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC
    GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG
    CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC
    AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG
    GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT
    CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT
    CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC
    AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC
    TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC
    CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC
    TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC
    TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG
    CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT
    GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA
    GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA
    GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC
    ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT
    GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC
    AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC
    CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT
    CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA
    GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA
    AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT
    CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT
    GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC
    TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT
    AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA
    AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA
    CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG
    GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG
    CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA
    AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA
    GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC
    CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA
    ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA
    TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT
    AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA
    GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA
    ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA
    GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC
    AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA
    GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC
    TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG
    GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA
    AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG
    TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT
    GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA
    CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG
    CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT
    TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT
    GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA
    GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC
    TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT
    ACCAAGGTGA CGGATGAAGG CAAGCAAACT GTTCCCCAAG ACCCCGAAGG CCCTTCAGTT
    CCCAGCAGTT CACCTGTGGC TGAGCAGTCG GTAGTAGTTC CTATGGCTGC AAAAGGGCCG
    CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC
    ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA
    CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA
    CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT
    AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC
    CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT
    GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT
    TTAAAACAGA GCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640892.1
    CDS444..6341
    Translation

    Target ORF information:

    RefSeq Version XM_005640835.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640835.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
    AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT
    GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA
    GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA
    GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG
    GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG
    ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA
    CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA
    GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA
    GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA
    CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT
    GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA
    GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG
    ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT
    GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA
    GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA
    GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT
    GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA
    AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT
    ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG
    GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG
    AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT
    GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA
    ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC
    GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT
    GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT
    GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT
    GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA
    GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA
    CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA
    GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA
    CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA
    GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA
    GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG
    GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG
    GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA
    CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC
    GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG
    CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC
    AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG
    GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT
    CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT
    CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC
    AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC
    TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC
    CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC
    TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC
    TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG
    CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT
    GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA
    GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA
    GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC
    ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT
    GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC
    AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC
    CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT
    CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA
    GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA
    AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT
    CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT
    GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC
    TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT
    AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA
    AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA
    CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG
    GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG
    CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA
    AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA
    GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC
    CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA
    ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA
    TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT
    AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA
    GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA
    ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA
    GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC
    AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA
    GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC
    TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG
    GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA
    AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG
    TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT
    GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA
    CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG
    CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT
    TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT
    GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA
    GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC
    TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT
    ACCAAGGTGA CGGATGAAGG CAAGCAAACT GTTCCCCAAG ACCCCGAAGG CCCTTCAGTT
    CCCAGCAGTT CACCTGTGGC TGAGCAGTCG GTAGTAGTTC CTATGGCTGC AAAAGGGCCG
    CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC
    ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA
    CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA
    CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT
    AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC
    CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT
    GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT
    TTAAAACAGA GCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31714
    Clone ID Related Accession (Same CDS sequence) XM_005640835.2 , XM_005640835.3
    Accession Version XM_005640835.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5898bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product transport and Golgi organization protein 1 homolog isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640835.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
    AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT
    GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA
    GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA
    GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG
    GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG
    ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA
    CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA
    GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA
    GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA
    CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT
    GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA
    GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG
    ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT
    GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA
    GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA
    GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT
    GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA
    AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT
    ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG
    GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG
    AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT
    GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA
    ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC
    GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT
    GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT
    GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT
    GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA
    GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA
    CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA
    GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA
    CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA
    GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA
    GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG
    GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG
    GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA
    CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC
    GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG
    CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC
    AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG
    GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT
    CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT
    CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC
    AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC
    TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC
    CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC
    TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC
    TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG
    CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT
    GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA
    GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA
    GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC
    ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT
    GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC
    AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC
    CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT
    CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA
    GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA
    AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT
    CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT
    GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC
    TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT
    AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA
    AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA
    CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG
    GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG
    CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA
    AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA
    GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC
    CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA
    ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA
    TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT
    AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA
    GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA
    ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA
    GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC
    AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA
    GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC
    TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG
    GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA
    AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG
    TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT
    GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA
    CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG
    CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT
    TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT
    GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA
    GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC
    TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT
    ACCAAGGTGA CGGATGAAGG CAAGCAAACT GTTCCCCAAG ACCCCGAAGG CCCTTCAGTT
    CCCAGCAGTT CACCTGTGGC TGAGCAGTCG GTAGTAGTTC CTATGGCTGC AAAAGGGCCG
    CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC
    ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA
    CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA
    CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT
    AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC
    CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT
    GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT
    TTAAAACAGA GCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640892.1
    CDS109..6006
    Translation

    Target ORF information:

    RefSeq Version XM_005640835.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640835.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
    AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT
    GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA
    GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA
    GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG
    GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG
    ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA
    CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA
    GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA
    GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA
    CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT
    GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA
    GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG
    ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT
    GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA
    GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA
    GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT
    GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA
    AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT
    ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG
    GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG
    AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT
    GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA
    ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC
    GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT
    GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT
    GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT
    GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA
    GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA
    CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA
    GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA
    CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA
    GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA
    GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG
    GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG
    GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA
    CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC
    GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG
    CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC
    AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG
    GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT
    CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT
    CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC
    AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC
    TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC
    CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC
    TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC
    TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG
    CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT
    GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA
    GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA
    GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC
    ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT
    GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC
    AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC
    CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT
    CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA
    GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA
    AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT
    CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT
    GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC
    TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT
    AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA
    AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA
    CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG
    GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG
    CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA
    AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA
    GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC
    CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA
    ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA
    TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT
    AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA
    GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA
    ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA
    GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC
    AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA
    GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC
    TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG
    GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA
    AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG
    TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT
    GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA
    CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG
    CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT
    TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT
    GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA
    GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC
    TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT
    ACCAAGGTGA CGGATGAAGG CAAGCAAACT GTTCCCCAAG ACCCCGAAGG CCCTTCAGTT
    CCCAGCAGTT CACCTGTGGC TGAGCAGTCG GTAGTAGTTC CTATGGCTGC AAAAGGGCCG
    CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC
    ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA
    CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA
    CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT
    AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC
    CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT
    GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT
    TTAAAACAGA GCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31715
    Clone ID Related Accession (Same CDS sequence) XM_005640836.2 , XM_005640836.3
    Accession Version XM_005640836.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product melanoma inhibitory activity protein 3 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640836.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
    AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT
    GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA
    GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA
    GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG
    GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG
    ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA
    CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA
    GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA
    GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA
    CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT
    GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA
    GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG
    ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT
    GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA
    GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA
    GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT
    GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA
    AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT
    ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG
    GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG
    AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT
    GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA
    ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC
    GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT
    GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT
    GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT
    GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA
    GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA
    CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA
    GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA
    CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA
    GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA
    GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG
    GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG
    GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA
    CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC
    GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG
    CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC
    AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG
    GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT
    CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT
    CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC
    AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC
    TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC
    CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC
    TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC
    TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG
    CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT
    GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA
    GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA
    GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC
    ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT
    GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC
    AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC
    CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT
    CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA
    GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA
    AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT
    CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT
    GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC
    TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT
    AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA
    AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA
    CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG
    GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG
    CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA
    AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA
    GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC
    CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA
    ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA
    TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT
    AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA
    GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA
    ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA
    GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC
    AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA
    GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC
    TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG
    GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA
    AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG
    TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT
    GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA
    CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG
    CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT
    TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT
    GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGATCTG
    GGATCTGGTG CAGCTCCCAC GATGAACAGC AGCTCGAGAA GCTCTTCCCC TACCAAGGTG
    ACGGATGAAG GCAAGCAAAC TGTTCCCCAA GACCCCGAAG GCCCTTCAGT TCCCAGCAGT
    TCACCTGTGG CTGAGCAGTC GGTAGTAGTT CCTATGGCTG CAAAAGGGCC GCCTCCTTTC
    CCAGGGGTGC CCCTCATGGG CTCCCCCGTG GGCGGCCCTC TACCACCACC CATTCGCTAC
    GGACCACCCC CTCAACTTTG CGGGCCTTTC GGGCCTCGGC CGCTTCCTCC ACCATTTGGT
    CCTGCTTTAC GTCCACCACT AGGCTTAAGA GAATACGCAC CAGGCATTCC ACCTGGAAAA
    CGGGACCTGT CTCTTGATCC TCGAGAATTT TTACCGGGAC ATGCACCTTT TAGACCTTTA
    GGCTCTTTTG GCCCAAGAGA GTACTTTATT CCCGGTACCC GACTACCACC CCCTGCCCAT
    GGTCCCCAGG ATTATCCACC TCCACCTGCT GCAAGAGACT TACTGCCTTC TGGCTCCAGA
    GACGAGCCTC CACCTGCCTC CCAGGGCAGT AGCCAGGACT GTTCACAGGC TTTAAAACAG
    AGCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640893.1
    CDS545..6373
    Translation

    Target ORF information:

    RefSeq Version XM_005640836.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640836.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
    AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT
    GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA
    GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA
    GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG
    GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG
    ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA
    CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA
    GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA
    GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA
    CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT
    GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA
    GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG
    ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT
    GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA
    GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA
    GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT
    GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA
    AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT
    ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG
    GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG
    AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT
    GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA
    ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC
    GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT
    GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT
    GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT
    GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA
    GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA
    CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA
    GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA
    CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA
    GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA
    GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG
    GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG
    GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA
    CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC
    GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG
    CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC
    AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG
    GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT
    CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT
    CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC
    AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC
    TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC
    CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC
    TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC
    TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG
    CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT
    GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA
    GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA
    GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC
    ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT
    GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC
    AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC
    CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT
    CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA
    GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA
    AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT
    CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT
    GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC
    TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT
    AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA
    AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA
    CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG
    GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG
    CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA
    AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA
    GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC
    CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA
    ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA
    TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT
    AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA
    GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA
    ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA
    GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC
    AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA
    GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC
    TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG
    GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA
    AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG
    TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT
    GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA
    CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG
    CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT
    TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT
    GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGATCTG
    GGATCTGGTG CAGCTCCCAC GATGAACAGC AGCTCGAGAA GCTCTTCCCC TACCAAGGTG
    ACGGATGAAG GCAAGCAAAC TGTTCCCCAA GACCCCGAAG GCCCTTCAGT TCCCAGCAGT
    TCACCTGTGG CTGAGCAGTC GGTAGTAGTT CCTATGGCTG CAAAAGGGCC GCCTCCTTTC
    CCAGGGGTGC CCCTCATGGG CTCCCCCGTG GGCGGCCCTC TACCACCACC CATTCGCTAC
    GGACCACCCC CTCAACTTTG CGGGCCTTTC GGGCCTCGGC CGCTTCCTCC ACCATTTGGT
    CCTGCTTTAC GTCCACCACT AGGCTTAAGA GAATACGCAC CAGGCATTCC ACCTGGAAAA
    CGGGACCTGT CTCTTGATCC TCGAGAATTT TTACCGGGAC ATGCACCTTT TAGACCTTTA
    GGCTCTTTTG GCCCAAGAGA GTACTTTATT CCCGGTACCC GACTACCACC CCCTGCCCAT
    GGTCCCCAGG ATTATCCACC TCCACCTGCT GCAAGAGACT TACTGCCTTC TGGCTCCAGA
    GACGAGCCTC CACCTGCCTC CCAGGGCAGT AGCCAGGACT GTTCACAGGC TTTAAAACAG
    AGCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31715
    Clone ID Related Accession (Same CDS sequence) XM_005640836.2 , XM_005640836.3
    Accession Version XM_005640836.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5829bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product transport and Golgi organization protein 1 homolog isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640836.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
    AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT
    GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA
    GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA
    GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG
    GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG
    ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA
    CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA
    GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA
    GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA
    CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT
    GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA
    GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG
    ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT
    GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA
    GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA
    GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT
    GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA
    AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT
    ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG
    GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG
    AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT
    GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA
    ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC
    GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT
    GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT
    GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT
    GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA
    GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA
    CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA
    GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA
    CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA
    GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA
    GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG
    GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG
    GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA
    CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC
    GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG
    CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC
    AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG
    GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT
    CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT
    CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC
    AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC
    TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC
    CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC
    TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC
    TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG
    CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT
    GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA
    GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA
    GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC
    ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT
    GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC
    AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC
    CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT
    CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA
    GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA
    AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT
    CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT
    GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC
    TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT
    AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA
    AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA
    CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG
    GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG
    CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA
    AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA
    GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC
    CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA
    ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA
    TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT
    AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA
    GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA
    ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA
    GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC
    AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA
    GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC
    TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG
    GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA
    AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG
    TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT
    GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA
    CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG
    CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT
    TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT
    GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGATCTG
    GGATCTGGTG CAGCTCCCAC GATGAACAGC AGCTCGAGAA GCTCTTCCCC TACCAAGGTG
    ACGGATGAAG GCAAGCAAAC TGTTCCCCAA GACCCCGAAG GCCCTTCAGT TCCCAGCAGT
    TCACCTGTGG CTGAGCAGTC GGTAGTAGTT CCTATGGCTG CAAAAGGGCC GCCTCCTTTC
    CCAGGGGTGC CCCTCATGGG CTCCCCCGTG GGCGGCCCTC TACCACCACC CATTCGCTAC
    GGACCACCCC CTCAACTTTG CGGGCCTTTC GGGCCTCGGC CGCTTCCTCC ACCATTTGGT
    CCTGCTTTAC GTCCACCACT AGGCTTAAGA GAATACGCAC CAGGCATTCC ACCTGGAAAA
    CGGGACCTGT CTCTTGATCC TCGAGAATTT TTACCGGGAC ATGCACCTTT TAGACCTTTA
    GGCTCTTTTG GCCCAAGAGA GTACTTTATT CCCGGTACCC GACTACCACC CCCTGCCCAT
    GGTCCCCAGG ATTATCCACC TCCACCTGCT GCAAGAGACT TACTGCCTTC TGGCTCCAGA
    GACGAGCCTC CACCTGCCTC CCAGGGCAGT AGCCAGGACT GTTCACAGGC TTTAAAACAG
    AGCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640893.1
    CDS109..5937
    Translation

    Target ORF information:

    RefSeq Version XM_005640836.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640836.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
    AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT
    GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA
    GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA
    GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG
    GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG
    ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA
    CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA
    GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA
    GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA
    CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT
    GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA
    GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG
    ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT
    GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA
    GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA
    GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT
    GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA
    AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT
    ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG
    GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG
    AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT
    GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA
    ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC
    GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT
    GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT
    GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT
    GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA
    GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA
    CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA
    GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA
    CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA
    GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA
    GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG
    GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG
    GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA
    CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC
    GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG
    CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC
    AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG
    GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT
    CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT
    CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC
    AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC
    TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC
    CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC
    TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC
    TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG
    CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT
    GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA
    GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA
    GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC
    ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT
    GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC
    AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC
    CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT
    CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA
    GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA
    AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT
    CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT
    GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC
    TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT
    AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA
    AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA
    CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG
    GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG
    CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA
    AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA
    GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC
    CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA
    ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA
    TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT
    AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA
    GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA
    ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA
    GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC
    AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA
    GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC
    TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG
    GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA
    AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG
    TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT
    GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA
    CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG
    CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT
    TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT
    GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGATCTG
    GGATCTGGTG CAGCTCCCAC GATGAACAGC AGCTCGAGAA GCTCTTCCCC TACCAAGGTG
    ACGGATGAAG GCAAGCAAAC TGTTCCCCAA GACCCCGAAG GCCCTTCAGT TCCCAGCAGT
    TCACCTGTGG CTGAGCAGTC GGTAGTAGTT CCTATGGCTG CAAAAGGGCC GCCTCCTTTC
    CCAGGGGTGC CCCTCATGGG CTCCCCCGTG GGCGGCCCTC TACCACCACC CATTCGCTAC
    GGACCACCCC CTCAACTTTG CGGGCCTTTC GGGCCTCGGC CGCTTCCTCC ACCATTTGGT
    CCTGCTTTAC GTCCACCACT AGGCTTAAGA GAATACGCAC CAGGCATTCC ACCTGGAAAA
    CGGGACCTGT CTCTTGATCC TCGAGAATTT TTACCGGGAC ATGCACCTTT TAGACCTTTA
    GGCTCTTTTG GCCCAAGAGA GTACTTTATT CCCGGTACCC GACTACCACC CCCTGCCCAT
    GGTCCCCAGG ATTATCCACC TCCACCTGCT GCAAGAGACT TACTGCCTTC TGGCTCCAGA
    GACGAGCCTC CACCTGCCTC CCAGGGCAGT AGCCAGGACT GTTCACAGGC TTTAAAACAG
    AGCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31716
    Clone ID Related Accession (Same CDS sequence) XM_005640837.2 , XM_005640837.3
    Accession Version XM_005640837.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5826bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product melanoma inhibitory activity protein 3 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640837.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
    AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT
    GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA
    GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA
    GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG
    GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG
    ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA
    CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA
    GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA
    GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA
    CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT
    GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA
    GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG
    ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT
    GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA
    GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA
    GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT
    GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA
    AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT
    ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG
    GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG
    AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT
    GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA
    ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC
    GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT
    GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT
    GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT
    GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA
    GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA
    CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA
    GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA
    CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA
    GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA
    GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG
    GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG
    GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA
    CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC
    GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG
    CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC
    AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG
    GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT
    CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT
    CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC
    AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC
    TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC
    CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC
    TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC
    TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG
    CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT
    GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA
    GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA
    GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC
    ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT
    GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC
    AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC
    CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT
    CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA
    GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA
    AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT
    CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT
    GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC
    TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT
    AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA
    AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA
    CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG
    GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG
    CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA
    AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA
    GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC
    CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA
    ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA
    TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT
    AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA
    GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA
    ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA
    GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC
    AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA
    GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC
    TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG
    GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA
    AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG
    TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT
    GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA
    CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG
    CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT
    TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT
    GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA
    GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC
    TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT
    ACCAAGGTGA CGGATGAAGG CAAGGTTCCT ATGGCTGCAA AAGGGCCGCC TCCTTTCCCA
    GGGGTGCCCC TCATGGGCTC CCCCGTGGGC GGCCCTCTAC CACCACCCAT TCGCTACGGA
    CCACCCCCTC AACTTTGCGG GCCTTTCGGG CCTCGGCCGC TTCCTCCACC ATTTGGTCCT
    GCTTTACGTC CACCACTAGG CTTAAGAGAA TACGCACCAG GCATTCCACC TGGAAAACGG
    GACCTGTCTC TTGATCCTCG AGAATTTTTA CCGGGACATG CACCTTTTAG ACCTTTAGGC
    TCTTTTGGCC CAAGAGAGTA CTTTATTCCC GGTACCCGAC TACCACCCCC TGCCCATGGT
    CCCCAGGATT ATCCACCTCC ACCTGCTGCA AGAGACTTAC TGCCTTCTGG CTCCAGAGAC
    GAGCCTCCAC CTGCCTCCCA GGGCAGTAGC CAGGACTGTT CACAGGCTTT AAAACAGAGC
    CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640894.1
    CDS439..6264
    Translation

    Target ORF information:

    RefSeq Version XM_005640837.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640837.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
    AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT
    GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA
    GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA
    GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG
    GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG
    ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA
    CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA
    GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA
    GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA
    CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT
    GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA
    GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG
    ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT
    GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA
    GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA
    GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT
    GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA
    AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT
    ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG
    GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG
    AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT
    GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA
    ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC
    GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT
    GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT
    GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT
    GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA
    GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA
    CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA
    GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA
    CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA
    GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA
    GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG
    GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG
    GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA
    CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC
    GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG
    CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC
    AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG
    GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT
    CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT
    CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC
    AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC
    TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC
    CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC
    TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC
    TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG
    CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT
    GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA
    GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA
    GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC
    ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT
    GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC
    AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC
    CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT
    CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA
    GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA
    AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT
    CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT
    GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC
    TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT
    AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA
    AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA
    CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG
    GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG
    CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA
    AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA
    GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC
    CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA
    ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA
    TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT
    AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA
    GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA
    ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA
    GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC
    AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA
    GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC
    TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG
    GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA
    AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG
    TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT
    GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA
    CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG
    CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT
    TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT
    GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA
    GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC
    TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT
    ACCAAGGTGA CGGATGAAGG CAAGGTTCCT ATGGCTGCAA AAGGGCCGCC TCCTTTCCCA
    GGGGTGCCCC TCATGGGCTC CCCCGTGGGC GGCCCTCTAC CACCACCCAT TCGCTACGGA
    CCACCCCCTC AACTTTGCGG GCCTTTCGGG CCTCGGCCGC TTCCTCCACC ATTTGGTCCT
    GCTTTACGTC CACCACTAGG CTTAAGAGAA TACGCACCAG GCATTCCACC TGGAAAACGG
    GACCTGTCTC TTGATCCTCG AGAATTTTTA CCGGGACATG CACCTTTTAG ACCTTTAGGC
    TCTTTTGGCC CAAGAGAGTA CTTTATTCCC GGTACCCGAC TACCACCCCC TGCCCATGGT
    CCCCAGGATT ATCCACCTCC ACCTGCTGCA AGAGACTTAC TGCCTTCTGG CTCCAGAGAC
    GAGCCTCCAC CTGCCTCCCA GGGCAGTAGC CAGGACTGTT CACAGGCTTT AAAACAGAGC
    CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe31716
    Clone ID Related Accession (Same CDS sequence) XM_005640837.2 , XM_005640837.3
    Accession Version XM_005640837.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5826bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product transport and Golgi organization protein 1 homolog isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640837.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
    AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT
    GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA
    GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA
    GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG
    GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG
    ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA
    CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA
    GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA
    GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA
    CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT
    GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA
    GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG
    ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT
    GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA
    GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA
    GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT
    GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA
    AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT
    ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG
    GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG
    AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT
    GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA
    ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC
    GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT
    GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT
    GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT
    GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA
    GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA
    CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA
    GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA
    CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA
    GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA
    GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG
    GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG
    GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA
    CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC
    GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG
    CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC
    AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG
    GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT
    CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT
    CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC
    AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC
    TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC
    CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC
    TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC
    TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG
    CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT
    GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA
    GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA
    GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC
    ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT
    GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC
    AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC
    CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT
    CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA
    GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA
    AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT
    CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT
    GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC
    TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT
    AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA
    AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA
    CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG
    GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG
    CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA
    AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA
    GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC
    CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA
    ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA
    TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT
    AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA
    GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA
    ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA
    GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC
    AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA
    GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC
    TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG
    GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA
    AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG
    TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT
    GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA
    CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG
    CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT
    TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT
    GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA
    GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC
    TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT
    ACCAAGGTGA CGGATGAAGG CAAGGTTCCT ATGGCTGCAA AAGGGCCGCC TCCTTTCCCA
    GGGGTGCCCC TCATGGGCTC CCCCGTGGGC GGCCCTCTAC CACCACCCAT TCGCTACGGA
    CCACCCCCTC AACTTTGCGG GCCTTTCGGG CCTCGGCCGC TTCCTCCACC ATTTGGTCCT
    GCTTTACGTC CACCACTAGG CTTAAGAGAA TACGCACCAG GCATTCCACC TGGAAAACGG
    GACCTGTCTC TTGATCCTCG AGAATTTTTA CCGGGACATG CACCTTTTAG ACCTTTAGGC
    TCTTTTGGCC CAAGAGAGTA CTTTATTCCC GGTACCCGAC TACCACCCCC TGCCCATGGT
    CCCCAGGATT ATCCACCTCC ACCTGCTGCA AGAGACTTAC TGCCTTCTGG CTCCAGAGAC
    GAGCCTCCAC CTGCCTCCCA GGGCAGTAGC CAGGACTGTT CACAGGCTTT AAAACAGAGC
    CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005640894.1
    CDS107..5932
    Translation

    Target ORF information:

    RefSeq Version XM_005640837.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005640837.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
    AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT
    GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA
    GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA
    GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG
    GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG
    ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA
    CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA
    GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA
    GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA
    CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT
    GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA
    GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG
    ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT
    GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA
    GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA
    GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT
    GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA
    AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT
    ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG
    GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG
    AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT
    GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA
    ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC
    GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT
    GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT
    GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT
    GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA
    GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA
    CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA
    GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA
    CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA
    GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA
    GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG
    GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG
    GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA
    CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC
    GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG
    CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC
    AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG
    GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT
    CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT
    CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC
    AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC
    TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC
    CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC
    TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC
    TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG
    CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT
    GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA
    GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA
    GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC
    ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT
    GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC
    AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC
    CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT
    CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA
    GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA
    AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT
    CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT
    GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC
    TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT
    AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA
    AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA
    CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG
    GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG
    CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA
    AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA
    GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC
    CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA
    ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA
    TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT
    AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA
    GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA
    ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA
    GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC
    AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA
    GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC
    TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG
    GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA
    AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG
    TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT
    GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA
    CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG
    CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT
    TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT
    GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA
    GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC
    TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT
    ACCAAGGTGA CGGATGAAGG CAAGGTTCCT ATGGCTGCAA AAGGGCCGCC TCCTTTCCCA
    GGGGTGCCCC TCATGGGCTC CCCCGTGGGC GGCCCTCTAC CACCACCCAT TCGCTACGGA
    CCACCCCCTC AACTTTGCGG GCCTTTCGGG CCTCGGCCGC TTCCTCCACC ATTTGGTCCT
    GCTTTACGTC CACCACTAGG CTTAAGAGAA TACGCACCAG GCATTCCACC TGGAAAACGG
    GACCTGTCTC TTGATCCTCG AGAATTTTTA CCGGGACATG CACCTTTTAG ACCTTTAGGC
    TCTTTTGGCC CAAGAGAGTA CTTTATTCCC GGTACCCGAC TACCACCCCC TGCCCATGGT
    CCCCAGGATT ATCCACCTCC ACCTGCTGCA AGAGACTTAC TGCCTTCTGG CTCCAGAGAC
    GAGCCTCCAC CTGCCTCCCA GGGCAGTAGC CAGGACTGTT CACAGGCTTT AAAACAGAGC
    CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45301
    Clone ID Related Accession (Same CDS sequence) XM_014111163.1
    Accession Version XM_014111163.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5757bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product melanoma inhibitory activity protein 3 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTACCGAG GTGAGGCTCT AGAAGATTTT ATAGGCCCAG ATTGTCGTTT CGTGAGTTTT 
    AAAAAAGGTG ATGCTGTGTA TGTCTACTAT AAACTGGCAG GGAGACCGCC TGAAGTTTGG
    GCCGGAAGTG TTGGGCGTAT TTTTGGATAT TTTCCAAAGG ATTTAATACA AGTAGTCCAT
    GAATATACTA AAGAGGAGCT ACAGGTTCCA ACAGATGAGA CAGATTTTGT TTGTTTTGAT
    GGGGGAAGAG ATGATTTTGA TAATTATAAT GTAGAAGAAC TTTTAGGATT TTTGGAATTA
    CACAATTCTG CAACTGAAGA GTCTGAGAAA GCCATAGAAG AAACTCAGTA TGAGGAGGAC
    ACCCCTCCTG AAGTATTGGA GGAAAGCGAC CTCGAACTAG AACCAGTAGA AGTTAACTCA
    GAGGAGAGTG AAAGTGTAGC CTCAGAAAAC ACTAAGGAAC TCAAGGAAAG ATCTGGGGCT
    CAGAAGAACC ACCCGCATGT AAACAGTCCA GCAGATCATG CTCAGGGAGA TCCATCTTCA
    TTTGAACCTT TTGAGGAAAT GCTACAAGAT AAACTAAAAG TGCCAGAAAG TGAAAACAAC
    AAAACCAGCA ATGGTTCTCA GGTCTCGAAT GAACAGCAGA CAATTGATGC TTATAAACTT
    TTGAAAAAAG AAATGACCCT GGACTTAAAA ACCAAATTTG GCTCCACTGC TGATGCACTT
    GTATCTGATG ATGAGACAAC CAGACTTGTT ACTTCATTAG AAGATGATTT TGATGAGGAA
    TTGGATGCTG AGTATCACAT AGTTGGCAAG GACGAAGAAG AGAATGAAGA CAACTTTGAC
    GAGCTGCCGT TACTGACCTT CGCACATGGT GGAGAAGATG TGAAAACTCC AGTGAAGTCC
    GGAGTTGAGA AATACTCAGC AGAGAGCGAG CAGAGTTCAA ATGAAGAGGA TAAGGTTGAG
    GCACCTCAGC CCCCTGGCAT CAAAAACTAT GATAAAGATA TATTAACAAC CTGGGAGGAT
    ACTGTCTTCT TCACAGAAGA TGACAGCGTA ACAAGTATGG ATTTGGGGAG TTCTGAATCA
    CAGGAAGGCA AGGAAGGTGA TGATGCGTTC ATCCCAGAGA GCAAATGGAG GAAACCACGG
    TCAGCAGCGG ATTACATTGA CCCTAAGAAG GCAGAAGATG GTCTTTTGCC TACAGCTAAC
    GATGTAGGTA CTAAAACAGA TAACGACGAA GACCTGGAAA TTCACATTGC AGGAAAAGGG
    AGGGGAGCCA AGGAACCCAA GCCGGGCCTG GCACAGGCCG AGATGGGGCC GGAGGAGGAG
    AAGCAGGAAG GCATGACCAC ACACGGTTCT ACTCATAGTG GTGACCTCGG CTCTTTGCCA
    TCTACTGGAA AGGGTAAAGA AATGTTGGAA TCAGCATTTG ATAACAAAGA AGATGACCTA
    AAAGGAGCGG CTATTCATAT TTCAAAAGGA ATGCTCCATG AAGAAAAGCC CGGAGAGCAG
    ATTTCAGAAG GGGGCTCGGA GCGTGAACCC CTCCCTCAAG CCACAGGGGG TCAAGTGAAC
    GAAGAAAAGC CTGACCAGGA GTCTGTGGGT ATCGCAGCAC CCTCTGGGGG TGGTCAGCAT
    AATGCCTCCA GGGACCGTGT GGAAGACGTC CACGCTTTAG CAGGATTGAA ACCACATACA
    CCGTCAGTGG AGCATCCAAA GGAGGAATTT AGAGAGGAAC GGCTTCTTAA AACTCAGAAT
    CAACCTAGGC CCTCCCCTCT GGATGGAGTT GGTTTGCCAG GAGAACTGAA AGAAGAGGGT
    CCCATTTGGG GAAGAAATCT TTCCTGGCCG CAAGGAGGAG AAGTGGCTGC TGCACATAGT
    AAGCAGATGG ATGAGAAGGG GAGGCTTGCC GAGGAGGAGG TCAGAGAGGA CTCCTCAGCT
    GAAGAGTTAG CTCCTCAACA GCCCAAGGCG GGCTCCCAGG AAGTGGAGCC CACAGGCCGG
    CCAGGTAGCC CGGAAGTGCC AGGTGTCCAT ACTGAGAAAC CAGAGGCAGA AGACGATCAC
    AGCCCTGAAG AACTCCTGGA GGATGAAAAT GCTCTCAGCG CAAAACAGGC TAAAGAAAAA
    AGCCCCGGGA TTCAGGGCAG GTGGTTAGAC GTTAACCTGC AGTCCCCCTC AAGAGCTCCT
    TTAGGTGTGG TTAATACCGA TGCAGAAATG GAAGGAAACA AAGAAGAAGC TAGTACTATT
    TTGGAAATAG AAAGAAAAGG TGAAACTTTT GGCAAAGAGG TAGAGCCAGG GGGCAGGGGC
    AGGGAAGCAG GTAGTGGGGT GGTGGAAACA GAAAGCCCTC TTGCAGGTGC AAAAGCACAG
    ACGGCATCTG GAGGAAGTGA CTCTCCTGAC AAGAAAAATC GGACTCCGGG GTTAGGTGAA
    ATGTTTCAGA ATCAAGACTC TGATTATTTT AAAGAAGACA GCGCTGAGAA ATCTGCACAG
    AAGCCTGGGG TAGAAGAACT CTCTGAAGAG GACCAGGACT TTGAGAGACC CGCGGACACA
    CAGAGCCAGG GCCCTGCCCC CGAGGAGCAG GAAGGTGGCC CGCTGCGCGG GACGCCACCC
    ACGGCGGTGA GGCCAATGCG TAGCGACTAC GTGAAGGACT TGCCCATAAT TGGCAGCTTC
    TTTAAAGAAC AACAGTCTCT GCAGAGGTTC CAGAAGTACT TCGATGTCCA CGAGCTGGAA
    GCCATGTTCC ACGAAATGTC ATTAAAGCTG AAGTTGGCGC AGCAGGAGAG CCTGCCTTAT
    AACGTGGAGA AAGTCCTAGA TAAGGTCTTC CGTGCATCTG AGTCACAGAT TCTGAGCACA
    GCAGAGAAAA TGCTTGATAC CCGTGTGGCT GAAAACAGAG ATCTGGGAAT GATGGAAAGT
    AACATATTTG AGGAGGCAGC CGTACTAGAC GATATTCAAG ACCTGATCTA TTTTGTCAGG
    TACAGACACT CCACCATGGA GGAGACTGCC CCGCTGGCCA CGGGACAGCC CCCCCAGGAA
    GGCTGGGGTG GCACGTTGGA AGGGATGCAG CCACACCTTG AAGATAATTT CCCACAAGGT
    CACACCAAAG ATAATTTCCC ACAAGACCAC ATAGAAGACA GTTTCCCACA AGATCACACA
    GAAGACAGTT TCCCACAAGA TCACACAGAA GACAATTTCC CACAAGATCA CACAGAAGAC
    CTTCAAATAC AGATTCTCAA AGAGCCCAGC CAGTTGGATC AACCTGTGGT TGGTGACATG
    GGTGCCTCAG AAGTGTCACA GCAGCCAAAT ACTGAGAAAG ATGGAGACCC AGGGATAATT
    ACAACAGAAA GCAGTCCTGT TGATGCTGAT GATGCAAAAA AGCAACTACA AACCAATGCT
    GAAGAGCCCG CAAGCGTCAC ACCTTTGGAA AATGCAATTC TTTTAATATA TTCATTCATA
    TTTTATTTAA CTAAGACGTT AGTTGCTACA TTGCCTGATG ACGTTCAGCC TGGGCCTGAT
    TTTTATGGCT TGCCATGGAA GCCTGTACTT CTCACTGCCT TCTTGGGAAT TGGTTCATTT
    GCGGTCTTCT TCTGGAAAAC TGTCCTTGTT GTAAAGAGTA GAGTCTATCA AGTCACTGAA
    CAACAAATTT CTGAGAAGTT GAAGAACATC ATGAAAGAAA ATGCAGAACT TGTACAAAAA
    TTGTCAAATT ATGAACAGAA GATTAAGGAA TCAAAAAAAC ATGTTCAAGA AACCAAGAAA
    CAAAATATAA TTCTGTCTGA TGAAGCAATT AAATTTAAGG ATAAAATCAA GAAACTTGAA
    GAAACAAATG AAATTCTGGA CGATACAGCT AAAAATCTGC GTGTTATGTT AGAATCTGAG
    AGAGAACAGA ATGCCAAGAA TCAGGACTTG ATACTGGAAA ACAAGAAATC TATAGAGAAG
    CTGAAGGATG TTATTTCAGT GAATGCCTCA GAATTTTCAG AGGTTCAAAT TGCCCTTAAT
    GAAGCTAAAC TTAGTGAAGA GAAGGTGAAG TCTGAATGCC ATCGAGTTCA AGAAGAAAAT
    GCTAGGCTTA AGAAGAAGAA GGAACAGTTG CAGCAAGAAA TCAAAGATTG GAGCAAATCA
    CACGCTGAGC TCAGTGAGCA AATCAAATCA TTTGAGACAT CTCAGAAAAA TTTGGAAGTA
    GCTCTTACTC ACAAAGATGA TAATATTAAT GCTTTGACTA ATTGCATTAC CCAGTTGAAT
    CGGTTAGATT GTGAATCTGA ATCTGAGGAT CAGAGTAAAG GAGGAAGTGA GTTAGATGAG
    TTAGCAAATG GAGAAGTAGG AGGTGACCGG AGTGAGAAAA TGAAAAATCA GATTAAGCAG
    ATGATGGACA TTTCTCGGAC ACAAACTGCA ATATCAGTAG TTGAAGAGGA TCTAAAACTT
    CTGCAGATTA AGCTTAGAGC CTCAATGTCT ACTAAATGCA ACCTGGAAGA CCAAATAAAG
    AAATTAGAAG ATGACCGTGA TTCACTGCAG TCTGCCAAAG CCGGCCTGGA GGACGAATGC
    AAAACCCTGA GGCAAAAAGT GGAGATTCTG AACGAACTCT ATCAACAGAA GGAAATGGCT
    CTGCAAAAGA AACTGAGTCA GGAAGAGTAT GAGCGGCAGG AGAGAGAGCA GCGGCTGTCA
    GCTGCAGACG AGAAGGCGCT TCTGGCTGCG GAGGAAGTAA AAACGTACAA GCGTAGAATC
    GAAGAGATGG AGGATGAGTT GCAGAAGACA GAGCGCTCGT TTAAAAACCA GATTGCGACT
    CATGAGAAGA AAGCTCATGA TAACTGGCTC AAAGCTCGTG CTGCAGAAAG AGCTATAGCC
    GAGGAGAAGA GGGAAGCTGC CAACTTGAGA CACAAGTTAC TGGAATTAAC CCAGAAGATG
    GCAATGCTGC AAGAGGAGCC TGTGATTGTG AAGCCGAGGC CGGGCCGACC CAGCGCCCAG
    AACCCTCCGT GGAGAGGTCC TTTGAGCCAG AACGGCTCTT TTGGTCCATC CCCTGTGAGT
    GGGGGGGAGT GCTCCCCACC TCCCACGGCT GACCTGCCTG CGAGACCGCT CTCTGCTACG
    CTGAATCGAA GAGAGATGCC TAGAGGTGAA TTTGGGTCAG TGGACGGGCC TCTACCTCGT
    CCTCGTTGGT CATCTGAGGC ATCTGGGAAA CCCTCTGCCT CTGATCTGGG ATCTGGTGCA
    GCTCCCACGA TGAACAGCAG CTCGAGAAGC TCTTCCCCTA CCAAGGTGAC GGATGAAGGC
    AAGCAAACTG TTCCCCAAGA CCCCGAAGGC CCTTCAGTTC CCAGCAGTTC ACCTGTGGCT
    GAGCAGTCGG TAGTAGTTCC TATGGCTGCA AAAGGGCCGC CTCCTTTCCC AGGGGTGCCC
    CTCATGGGCT CCCCCGTGGG CGGCCCTCTA CCACCACCCA TTCGCTACGG ACCACCCCCT
    CAACTTTGCG GGCCTTTCGG GCCTCGGCCG CTTCCTCCAC CATTTGGTCC TGCTTTACGT
    CCACCACTAG GCTTAAGAGA ATACGCACCA GGCATTCCAC CTGGAAAACG GGACCTGTCT
    CTTGATCCTC GAGAATTTTT ACCGGGACAT GCACCTTTTA GACCTTTAGG CTCTTTTGGC
    CCAAGAGAGT ACTTTATTCC CGGTACCCGA CTACCACCCC CTGCCCATGG TCCCCAGGAT
    TATCCACCTC CACCTGCTGC AAGAGACTTA CTGCCTTCTG GCTCCAGAGA CGAGCCTCCA
    CCTGCCTCCC AGGGCAGTAG CCAGGACTGT TCACAGGCTT TAAAACAGAG CCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966638.1
    CDS68..5824
    Translation

    Target ORF information:

    RefSeq Version XM_014111163.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014111163.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    ATGTACCGAG GTGAGGCTCT AGAAGATTTT ATAGGCCCAG ATTGTCGTTT CGTGAGTTTT 
    AAAAAAGGTG ATGCTGTGTA TGTCTACTAT AAACTGGCAG GGAGACCGCC TGAAGTTTGG
    GCCGGAAGTG TTGGGCGTAT TTTTGGATAT TTTCCAAAGG ATTTAATACA AGTAGTCCAT
    GAATATACTA AAGAGGAGCT ACAGGTTCCA ACAGATGAGA CAGATTTTGT TTGTTTTGAT
    GGGGGAAGAG ATGATTTTGA TAATTATAAT GTAGAAGAAC TTTTAGGATT TTTGGAATTA
    CACAATTCTG CAACTGAAGA GTCTGAGAAA GCCATAGAAG AAACTCAGTA TGAGGAGGAC
    ACCCCTCCTG AAGTATTGGA GGAAAGCGAC CTCGAACTAG AACCAGTAGA AGTTAACTCA
    GAGGAGAGTG AAAGTGTAGC CTCAGAAAAC ACTAAGGAAC TCAAGGAAAG ATCTGGGGCT
    CAGAAGAACC ACCCGCATGT AAACAGTCCA GCAGATCATG CTCAGGGAGA TCCATCTTCA
    TTTGAACCTT TTGAGGAAAT GCTACAAGAT AAACTAAAAG TGCCAGAAAG TGAAAACAAC
    AAAACCAGCA ATGGTTCTCA GGTCTCGAAT GAACAGCAGA CAATTGATGC TTATAAACTT
    TTGAAAAAAG AAATGACCCT GGACTTAAAA ACCAAATTTG GCTCCACTGC TGATGCACTT
    GTATCTGATG ATGAGACAAC CAGACTTGTT ACTTCATTAG AAGATGATTT TGATGAGGAA
    TTGGATGCTG AGTATCACAT AGTTGGCAAG GACGAAGAAG AGAATGAAGA CAACTTTGAC
    GAGCTGCCGT TACTGACCTT CGCACATGGT GGAGAAGATG TGAAAACTCC AGTGAAGTCC
    GGAGTTGAGA AATACTCAGC AGAGAGCGAG CAGAGTTCAA ATGAAGAGGA TAAGGTTGAG
    GCACCTCAGC CCCCTGGCAT CAAAAACTAT GATAAAGATA TATTAACAAC CTGGGAGGAT
    ACTGTCTTCT TCACAGAAGA TGACAGCGTA ACAAGTATGG ATTTGGGGAG TTCTGAATCA
    CAGGAAGGCA AGGAAGGTGA TGATGCGTTC ATCCCAGAGA GCAAATGGAG GAAACCACGG
    TCAGCAGCGG ATTACATTGA CCCTAAGAAG GCAGAAGATG GTCTTTTGCC TACAGCTAAC
    GATGTAGGTA CTAAAACAGA TAACGACGAA GACCTGGAAA TTCACATTGC AGGAAAAGGG
    AGGGGAGCCA AGGAACCCAA GCCGGGCCTG GCACAGGCCG AGATGGGGCC GGAGGAGGAG
    AAGCAGGAAG GCATGACCAC ACACGGTTCT ACTCATAGTG GTGACCTCGG CTCTTTGCCA
    TCTACTGGAA AGGGTAAAGA AATGTTGGAA TCAGCATTTG ATAACAAAGA AGATGACCTA
    AAAGGAGCGG CTATTCATAT TTCAAAAGGA ATGCTCCATG AAGAAAAGCC CGGAGAGCAG
    ATTTCAGAAG GGGGCTCGGA GCGTGAACCC CTCCCTCAAG CCACAGGGGG TCAAGTGAAC
    GAAGAAAAGC CTGACCAGGA GTCTGTGGGT ATCGCAGCAC CCTCTGGGGG TGGTCAGCAT
    AATGCCTCCA GGGACCGTGT GGAAGACGTC CACGCTTTAG CAGGATTGAA ACCACATACA
    CCGTCAGTGG AGCATCCAAA GGAGGAATTT AGAGAGGAAC GGCTTCTTAA AACTCAGAAT
    CAACCTAGGC CCTCCCCTCT GGATGGAGTT GGTTTGCCAG GAGAACTGAA AGAAGAGGGT
    CCCATTTGGG GAAGAAATCT TTCCTGGCCG CAAGGAGGAG AAGTGGCTGC TGCACATAGT
    AAGCAGATGG ATGAGAAGGG GAGGCTTGCC GAGGAGGAGG TCAGAGAGGA CTCCTCAGCT
    GAAGAGTTAG CTCCTCAACA GCCCAAGGCG GGCTCCCAGG AAGTGGAGCC CACAGGCCGG
    CCAGGTAGCC CGGAAGTGCC AGGTGTCCAT ACTGAGAAAC CAGAGGCAGA AGACGATCAC
    AGCCCTGAAG AACTCCTGGA GGATGAAAAT GCTCTCAGCG CAAAACAGGC TAAAGAAAAA
    AGCCCCGGGA TTCAGGGCAG GTGGTTAGAC GTTAACCTGC AGTCCCCCTC AAGAGCTCCT
    TTAGGTGTGG TTAATACCGA TGCAGAAATG GAAGGAAACA AAGAAGAAGC TAGTACTATT
    TTGGAAATAG AAAGAAAAGG TGAAACTTTT GGCAAAGAGG TAGAGCCAGG GGGCAGGGGC
    AGGGAAGCAG GTAGTGGGGT GGTGGAAACA GAAAGCCCTC TTGCAGGTGC AAAAGCACAG
    ACGGCATCTG GAGGAAGTGA CTCTCCTGAC AAGAAAAATC GGACTCCGGG GTTAGGTGAA
    ATGTTTCAGA ATCAAGACTC TGATTATTTT AAAGAAGACA GCGCTGAGAA ATCTGCACAG
    AAGCCTGGGG TAGAAGAACT CTCTGAAGAG GACCAGGACT TTGAGAGACC CGCGGACACA
    CAGAGCCAGG GCCCTGCCCC CGAGGAGCAG GAAGGTGGCC CGCTGCGCGG GACGCCACCC
    ACGGCGGTGA GGCCAATGCG TAGCGACTAC GTGAAGGACT TGCCCATAAT TGGCAGCTTC
    TTTAAAGAAC AACAGTCTCT GCAGAGGTTC CAGAAGTACT TCGATGTCCA CGAGCTGGAA
    GCCATGTTCC ACGAAATGTC ATTAAAGCTG AAGTTGGCGC AGCAGGAGAG CCTGCCTTAT
    AACGTGGAGA AAGTCCTAGA TAAGGTCTTC CGTGCATCTG AGTCACAGAT TCTGAGCACA
    GCAGAGAAAA TGCTTGATAC CCGTGTGGCT GAAAACAGAG ATCTGGGAAT GATGGAAAGT
    AACATATTTG AGGAGGCAGC CGTACTAGAC GATATTCAAG ACCTGATCTA TTTTGTCAGG
    TACAGACACT CCACCATGGA GGAGACTGCC CCGCTGGCCA CGGGACAGCC CCCCCAGGAA
    GGCTGGGGTG GCACGTTGGA AGGGATGCAG CCACACCTTG AAGATAATTT CCCACAAGGT
    CACACCAAAG ATAATTTCCC ACAAGACCAC ATAGAAGACA GTTTCCCACA AGATCACACA
    GAAGACAGTT TCCCACAAGA TCACACAGAA GACAATTTCC CACAAGATCA CACAGAAGAC
    CTTCAAATAC AGATTCTCAA AGAGCCCAGC CAGTTGGATC AACCTGTGGT TGGTGACATG
    GGTGCCTCAG AAGTGTCACA GCAGCCAAAT ACTGAGAAAG ATGGAGACCC AGGGATAATT
    ACAACAGAAA GCAGTCCTGT TGATGCTGAT GATGCAAAAA AGCAACTACA AACCAATGCT
    GAAGAGCCCG CAAGCGTCAC ACCTTTGGAA AATGCAATTC TTTTAATATA TTCATTCATA
    TTTTATTTAA CTAAGACGTT AGTTGCTACA TTGCCTGATG ACGTTCAGCC TGGGCCTGAT
    TTTTATGGCT TGCCATGGAA GCCTGTACTT CTCACTGCCT TCTTGGGAAT TGGTTCATTT
    GCGGTCTTCT TCTGGAAAAC TGTCCTTGTT GTAAAGAGTA GAGTCTATCA AGTCACTGAA
    CAACAAATTT CTGAGAAGTT GAAGAACATC ATGAAAGAAA ATGCAGAACT TGTACAAAAA
    TTGTCAAATT ATGAACAGAA GATTAAGGAA TCAAAAAAAC ATGTTCAAGA AACCAAGAAA
    CAAAATATAA TTCTGTCTGA TGAAGCAATT AAATTTAAGG ATAAAATCAA GAAACTTGAA
    GAAACAAATG AAATTCTGGA CGATACAGCT AAAAATCTGC GTGTTATGTT AGAATCTGAG
    AGAGAACAGA ATGCCAAGAA TCAGGACTTG ATACTGGAAA ACAAGAAATC TATAGAGAAG
    CTGAAGGATG TTATTTCAGT GAATGCCTCA GAATTTTCAG AGGTTCAAAT TGCCCTTAAT
    GAAGCTAAAC TTAGTGAAGA GAAGGTGAAG TCTGAATGCC ATCGAGTTCA AGAAGAAAAT
    GCTAGGCTTA AGAAGAAGAA GGAACAGTTG CAGCAAGAAA TCAAAGATTG GAGCAAATCA
    CACGCTGAGC TCAGTGAGCA AATCAAATCA TTTGAGACAT CTCAGAAAAA TTTGGAAGTA
    GCTCTTACTC ACAAAGATGA TAATATTAAT GCTTTGACTA ATTGCATTAC CCAGTTGAAT
    CGGTTAGATT GTGAATCTGA ATCTGAGGAT CAGAGTAAAG GAGGAAGTGA GTTAGATGAG
    TTAGCAAATG GAGAAGTAGG AGGTGACCGG AGTGAGAAAA TGAAAAATCA GATTAAGCAG
    ATGATGGACA TTTCTCGGAC ACAAACTGCA ATATCAGTAG TTGAAGAGGA TCTAAAACTT
    CTGCAGATTA AGCTTAGAGC CTCAATGTCT ACTAAATGCA ACCTGGAAGA CCAAATAAAG
    AAATTAGAAG ATGACCGTGA TTCACTGCAG TCTGCCAAAG CCGGCCTGGA GGACGAATGC
    AAAACCCTGA GGCAAAAAGT GGAGATTCTG AACGAACTCT ATCAACAGAA GGAAATGGCT
    CTGCAAAAGA AACTGAGTCA GGAAGAGTAT GAGCGGCAGG AGAGAGAGCA GCGGCTGTCA
    GCTGCAGACG AGAAGGCGCT TCTGGCTGCG GAGGAAGTAA AAACGTACAA GCGTAGAATC
    GAAGAGATGG AGGATGAGTT GCAGAAGACA GAGCGCTCGT TTAAAAACCA GATTGCGACT
    CATGAGAAGA AAGCTCATGA TAACTGGCTC AAAGCTCGTG CTGCAGAAAG AGCTATAGCC
    GAGGAGAAGA GGGAAGCTGC CAACTTGAGA CACAAGTTAC TGGAATTAAC CCAGAAGATG
    GCAATGCTGC AAGAGGAGCC TGTGATTGTG AAGCCGAGGC CGGGCCGACC CAGCGCCCAG
    AACCCTCCGT GGAGAGGTCC TTTGAGCCAG AACGGCTCTT TTGGTCCATC CCCTGTGAGT
    GGGGGGGAGT GCTCCCCACC TCCCACGGCT GACCTGCCTG CGAGACCGCT CTCTGCTACG
    CTGAATCGAA GAGAGATGCC TAGAGGTGAA TTTGGGTCAG TGGACGGGCC TCTACCTCGT
    CCTCGTTGGT CATCTGAGGC ATCTGGGAAA CCCTCTGCCT CTGATCTGGG ATCTGGTGCA
    GCTCCCACGA TGAACAGCAG CTCGAGAAGC TCTTCCCCTA CCAAGGTGAC GGATGAAGGC
    AAGCAAACTG TTCCCCAAGA CCCCGAAGGC CCTTCAGTTC CCAGCAGTTC ACCTGTGGCT
    GAGCAGTCGG TAGTAGTTCC TATGGCTGCA AAAGGGCCGC CTCCTTTCCC AGGGGTGCCC
    CTCATGGGCT CCCCCGTGGG CGGCCCTCTA CCACCACCCA TTCGCTACGG ACCACCCCCT
    CAACTTTGCG GGCCTTTCGG GCCTCGGCCG CTTCCTCCAC CATTTGGTCC TGCTTTACGT
    CCACCACTAG GCTTAAGAGA ATACGCACCA GGCATTCCAC CTGGAAAACG GGACCTGTCT
    CTTGATCCTC GAGAATTTTT ACCGGGACAT GCACCTTTTA GACCTTTAGG CTCTTTTGGC
    CCAAGAGAGT ACTTTATTCC CGGTACCCGA CTACCACCCC CTGCCCATGG TCCCCAGGAT
    TATCCACCTC CACCTGCTGC AAGAGACTTA CTGCCTTCTG GCTCCAGAGA CGAGCCTCCA
    CCTGCCTCCC AGGGCAGTAG CCAGGACTGT TCACAGGCTT TAAAACAGAG CCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.