MIA3 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_198551.3	NP_940953.2	transport and Golgi organization protein 1 homolog isoform 1 precursor
XM_006711304.3	XP_006711367.2	melanoma inhibitory activity protein 3 isoform X1
NM_001324063.1	NP_001310992.1	transport and Golgi organization protein 1 homolog isoform 4 precursor
XM_011509513.3	XP_011507815.3	transport and Golgi organization protein 1 homolog isoform X3
XM_017001243.1	XP_016856732.1	melanoma inhibitory activity protein 3 isoform X2
NM_001300867.1	NP_001287796.1	transport and Golgi organization protein 1 homolog isoform 2
NM_001324065.1	NP_001310994.1	transport and Golgi organization protein 1 homolog isoform 6
NM_001324064.1	NP_001310993.1	transport and Golgi organization protein 1 homolog isoform 5
XM_011509513.2	XP_011507815.2	melanoma inhibitory activity protein 3 isoform X3
NM_198551.4	NP_940953.2	transport and Golgi organization protein 1 homolog isoform 1 precursor
XM_006711304.4	XP_006711367.3	transport and Golgi organization protein 1 homolog isoform X1
NM_001324062.1	NP_001310991.1	transport and Golgi organization protein 1 homolog isoform 3 precursor
XM_017001243.2	XP_016856732.1	transport and Golgi organization protein 1 homolog isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu34360	NM_001300867.1 Latest version!	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu100270	NM_001324065.1 Latest version!	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu100260	NM_001324064.1 Latest version!	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$951.30 $1359.00
OHu100264	NM_001324063.1 Latest version!	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu34359	XM_006711304.4 Latest version!	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu79727	XM_011509513.3 Latest version!	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu87371	XM_017001243.2 Latest version!	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu13395	NM_198551.4 Latest version!	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu34358	NM_001324062.1 Latest version!	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu87370	XM_006711304.3 Latest version!	Homo sapiens melanoma inhibitory activity family member 3 (MIA3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu87372	XM_011509513.2 Latest version!	Homo sapiens melanoma inhibitory activity family member 3 (MIA3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
View Old Accession Versions >>
OHu13395	NM_198551.3	Homo sapiens MIA family member 3, ER export factor (MIA3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu87371	XM_017001243.1	Homo sapiens melanoma inhibitory activity family member 3 (MIA3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

MIA3 ( NM_001300867.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287796.1 Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu34360
Clone ID Related Accession (Same CDS sequence)	NM_001300867.1
Accession Version	NM_001300867.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	transport and Golgi organization protein 1 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BG429750.1, AK296712.1, BC047116.1 and AL592148.7. On Jul 15, 2014 this sequence version replaced XM_005273122.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.192906.1, SRR1803612.202539.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTCAG TACCTGCCAC TGTGCCTTCT ATCGCCGCTA CCCCGGGGGA CCCGGAACTT 
GTGGGACCCT TGTCTGTGCT CTACGCAGCC TTCATAGCCA AGCTGCTGGA GCTAGTTGCT 
ACATTGCCTG ATGATGTTCA GCCTGGGCCT GATTTTTATG GACTGCCATG GAAACCTGTA 
TTTATCACTG CCTTCTTGGG AATTGCTTCG TTTGCCATTT TCTTATGGAG AACTGTCCTT 
GTTGTGAAGG ATAGAGTATA TCAAGTCACG GAACAGCAAA TTTCTGAGAA GTTGAAGACT 
ATCATGAAAG AAAATACAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATCAAG 
GAATCAAAGA AACATGTTCA GGAAACCAGG AAACAAAATA TGATTCTCTC TGATGAAGCA 
ATTAAATATA AGGATAAAAT CAAGACACTT GAAAAAAATC AGGAAATTCT GGATGACACA 
GCTAAAAATC TTCGTGTTAT GCTAGAATCT GAGAGAGAAC AGAATGTCAA GAATCAGGAC 
TTGATATCAG AAAACAAGAA ATCTATAGAG AAGTTAAAGG ATGTTATTTC AATGAATGCC 
TCAGAATTTT CAGAGGTTCA GATTGCACTT AATGAAGCTA AGCTTAGTGA AGAGAAGGTG 
AAGTCTGAAT GCCATCGGGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA AAAAGAGCAG 
TTGCAGCAGG AAATCGAAGA CTGGAGTAAA TTACATGCTG AGCTCAGTGA GCAAATCAAA 
TCATTTGAGA AGTCTCAGAA AGATTTGGAA GTAGCTCTTA CTCACAAGGA TGATAATATT 
AATGCTTTGA CTAACTGCAT TACACAGTTG AATCTGTTAG AGTGTGAATC TGAATCTGAG 
GGTCAAAATA AAGGTGGAAA TGATTCAGAT GAATTAGCAA ATGGAGAAGT GGGAGGTGAC 
CGGAATGAGA AGATGAAAAA TCAAATTAAG CAGATGATGG ATGTCTCTCG GACACAGACT 
GCAATATCGG TAGTTGAAGA GGATCTAAAG CTTTTACAGC TTAAGCTAAG AGCCTCCGTG 
TCCACTAAAT GTAACCTGGA AGACCAGGTA AAGAAATTGG AAGATGACCG CAACTCACTA 
CAAGCTGCCA AAGCTGGACT GGAAGATGAA TGCAAAACCT TGAGGCAGAA AGTGGAGATT 
CTGAATGAGC TCTATCAGCA GAAGGAGATG GCTTTGCAAA AGAAACTGAG TCAAGAAGAG 
TATGAACGGC AAGAAAGAGA GCACAGGCTG TCAGCTGCAG ATGAAAAGGC AGTTTCGGCT 
GCAGAGGAAG TAAAAACTTA CAAGCGGAGA ATTGAAGAAA TGGAGGATGA ATTACAGAAG 
ACAGAGCGGT CATTTAAAAA CCAGATCGCT ACCCATGAGA AGAAAGCTCA TGAAAACTGG 
CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCTGAAGAGA AAAGGGAAGC TGCCAATTTG 
AGACACAAAT TATTAGAATT AACACAAAAG ATGGCAATGC TGCAAGAAGA ACCTGTGATT 
GTAAAACCAA TGCCAGGAAA ACCAAATACA CAAAACCCTC CACGGAGAGG TCCTCTGAGC 
CAGAATGGCT CTTTTGGCCC ATCCCCTGTG AGTGGTGGAG AATGCTCCCC TCCATTGACA 
GTGGAGCCAC CCGTGAGACC TCTCTCTGCT ACTCTCAATC GAAGAGATAT GCCTAGAAGT 
GAATTTGGAT CAGTGGACGG GCCTCTACCT CATCCTCGAT GGTCAGCTGA GGCATCTGGG 
AAACCCTCTC CTTCTGATCC AGGATCTGGT ACAGCTACCA TGATGAACAG CAGCTCAAGA 
GGCTCTTCCC CTACCAGGGT ACTCGATGAA GGCAAGGTTA ATATGGCTCC AAAAGGGCCC 
CCTCCTTTCC CAGGAGTCCC TCTCATGAGC ACCCCCATGG GAGGCCCTGT ACCACCACCC 
ATTCGATATG GACCACCACC TCAGCTCTGC GGACCTTTTG GGCCTCGGCC ACTTCCTCCA 
CCCTTTGGCC CTGGTATGCG TCCACCACTA GGCTTAAGAG AATTTGCACC AGGCGTTCCA 
CCAGGAAGAC GGGACCTGCC TCTCCACCCT CGGGGATTTT TACCTGGACA CGCACCATTT 
AGACCTTTAG GTTCACTTGG CCCAAGAGAG TACTTTATTC CTGGTACCCG ATTACCACCC 
CCAACCCATG GTCCCCAGGA ATACCCACCA CCACCTGCTG TAAGAGACTT ACTGCCGTCA 
GGCTCTAGAG ATGAGCCTCC ACCTGCCTCT CAGAGCACTA GCCAGGACTG TTCACAGGCT 
TTAAAACAGA GCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287796.1
CDS	174..2531
Translation	MDSVPATVPSIAATPGDPELVGPLSVLYAAFIAKLLELVATLPDDVQPGPDFYGLPWKPVFITAFLGIASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLISENKKSIEKLKDVISMNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREHRLSAADEKAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGKPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

Target ORF information:

RefSeq Version	NM_001300867.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001300867.1

ORF Insert Sequence:

ATGGACTCAG TACCTGCCAC TGTGCCTTCT ATCGCCGCTA CCCCGGGGGA CCCGGAACTT 
GTGGGACCCT TGTCTGTGCT CTACGCAGCC TTCATAGCCA AGCTGCTGGA GCTAGTTGCT 
ACATTGCCTG ATGATGTTCA GCCTGGGCCT GATTTTTATG GACTGCCATG GAAACCTGTA 
TTTATCACTG CCTTCTTGGG AATTGCTTCG TTTGCCATTT TCTTATGGAG AACTGTCCTT 
GTTGTGAAGG ATAGAGTATA TCAAGTCACG GAACAGCAAA TTTCTGAGAA GTTGAAGACT 
ATCATGAAAG AAAATACAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATCAAG 
GAATCAAAGA AACATGTTCA GGAAACCAGG AAACAAAATA TGATTCTCTC TGATGAAGCA 
ATTAAATATA AGGATAAAAT CAAGACACTT GAAAAAAATC AGGAAATTCT GGATGACACA 
GCTAAAAATC TTCGTGTTAT GCTAGAATCT GAGAGAGAAC AGAATGTCAA GAATCAGGAC 
TTGATATCAG AAAACAAGAA ATCTATAGAG AAGTTAAAGG ATGTTATTTC AATGAATGCC 
TCAGAATTTT CAGAGGTTCA GATTGCACTT AATGAAGCTA AGCTTAGTGA AGAGAAGGTG 
AAGTCTGAAT GCCATCGGGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA AAAAGAGCAG 
TTGCAGCAGG AAATCGAAGA CTGGAGTAAA TTACATGCTG AGCTCAGTGA GCAAATCAAA 
TCATTTGAGA AGTCTCAGAA AGATTTGGAA GTAGCTCTTA CTCACAAGGA TGATAATATT 
AATGCTTTGA CTAACTGCAT TACACAGTTG AATCTGTTAG AGTGTGAATC TGAATCTGAG 
GGTCAAAATA AAGGTGGAAA TGATTCAGAT GAATTAGCAA ATGGAGAAGT GGGAGGTGAC 
CGGAATGAGA AGATGAAAAA TCAAATTAAG CAGATGATGG ATGTCTCTCG GACACAGACT 
GCAATATCGG TAGTTGAAGA GGATCTAAAG CTTTTACAGC TTAAGCTAAG AGCCTCCGTG 
TCCACTAAAT GTAACCTGGA AGACCAGGTA AAGAAATTGG AAGATGACCG CAACTCACTA 
CAAGCTGCCA AAGCTGGACT GGAAGATGAA TGCAAAACCT TGAGGCAGAA AGTGGAGATT 
CTGAATGAGC TCTATCAGCA GAAGGAGATG GCTTTGCAAA AGAAACTGAG TCAAGAAGAG 
TATGAACGGC AAGAAAGAGA GCACAGGCTG TCAGCTGCAG ATGAAAAGGC AGTTTCGGCT 
GCAGAGGAAG TAAAAACTTA CAAGCGGAGA ATTGAAGAAA TGGAGGATGA ATTACAGAAG 
ACAGAGCGGT CATTTAAAAA CCAGATCGCT ACCCATGAGA AGAAAGCTCA TGAAAACTGG 
CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCTGAAGAGA AAAGGGAAGC TGCCAATTTG 
AGACACAAAT TATTAGAATT AACACAAAAG ATGGCAATGC TGCAAGAAGA ACCTGTGATT 
GTAAAACCAA TGCCAGGAAA ACCAAATACA CAAAACCCTC CACGGAGAGG TCCTCTGAGC 
CAGAATGGCT CTTTTGGCCC ATCCCCTGTG AGTGGTGGAG AATGCTCCCC TCCATTGACA 
GTGGAGCCAC CCGTGAGACC TCTCTCTGCT ACTCTCAATC GAAGAGATAT GCCTAGAAGT 
GAATTTGGAT CAGTGGACGG GCCTCTACCT CATCCTCGAT GGTCAGCTGA GGCATCTGGG 
AAACCCTCTC CTTCTGATCC AGGATCTGGT ACAGCTACCA TGATGAACAG CAGCTCAAGA 
GGCTCTTCCC CTACCAGGGT ACTCGATGAA GGCAAGGTTA ATATGGCTCC AAAAGGGCCC 
CCTCCTTTCC CAGGAGTCCC TCTCATGAGC ACCCCCATGG GAGGCCCTGT ACCACCACCC 
ATTCGATATG GACCACCACC TCAGCTCTGC GGACCTTTTG GGCCTCGGCC ACTTCCTCCA 
CCCTTTGGCC CTGGTATGCG TCCACCACTA GGCTTAAGAG AATTTGCACC AGGCGTTCCA 
CCAGGAAGAC GGGACCTGCC TCTCCACCCT CGGGGATTTT TACCTGGACA CGCACCATTT 
AGACCTTTAG GTTCACTTGG CCCAAGAGAG TACTTTATTC CTGGTACCCG ATTACCACCC 
CCAACCCATG GTCCCCAGGA ATACCCACCA CCACCTGCTG TAAGAGACTT ACTGCCGTCA 
GGCTCTAGAG ATGAGCCTCC ACCTGCCTCT CAGAGCACTA GCCAGGACTG TTCACAGGCT 
TTAAAACAGA GCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( NM_001324065.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310994.1 Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu100270
Clone ID Related Accession (Same CDS sequence)	NM_001324065.1
Accession Version	NM_001324065.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	transport and Golgi organization protein 1 homolog isoform 6
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592148.7 and KF459535.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.231967.1, SRR1803612.62326.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACTCAG TACCTGCCAC TGTGCCTTCT ATCGCCGCTA CCCCGGGGGA CCCGGAACTT 
GTGGGACCCT TGTCTGTGCT CTACGCAGCC TTCATAGCCA AGCTGCTGGA GCTAGTTGCT 
ACATTGCCTG ATGATGTTCA GCCTGGGCCT GATTTTTATG GACTGCCATG GAAACCTGTA 
TTTATCACTG CCTTCTTGGG AATTGCTTCG TTTGCCATTT TCTTATGGAG AACTGTCCTT 
GTTGTGAAGG ATAGAGTATA TCAAGTCACG GAACAGCAAA TTTCTGAGAA GTTGAAGACT 
ATCATGAAAG AAAATACAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATCAAG 
GAATCAAAGA AACATGTTCA GGAAACCAGG AAACAAAATA TGATTCTCTC TGATGAAGCA 
ATTAAATATA AGGATAAAAT CAAGACACTT GAAAAAAATC AGGAAATTCT GGATGACACA 
GCTAAAAATC TTCGTGTTAT GCTAGAATCT GAGAGAGAAC AGAATGTCAA GAATCAGGAC 
TTGATATCAG AAAACAAGAA ATCTATAGAG AAGTTAAAGG ATGTTATTTC AATGAATGCC 
TCAGAATTTT CAGAGGTTCA GATTGCACTT AATGAAGCTA AGCTTAGTGA AGAGAAGGTG 
AAGTCTGAAT GCCATCGGGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA AAAAGAGCAG 
TTGCAGCAGG AAATCGAAGA CTGGAGTAAA TTACATGCTG AGCTCAGTGA GCAAATCAAA 
TCATTTGAGA AGTCTCAGAA AGATTTGGAA GTAGCTCTTA CTCACAAGGA TGATAATATT 
AATGCTTTGA CTAACTGCAT TACACAGTTG AATCTGTTAG AGTGTGAATC TGAATCTGAG 
GGTCAAAATA AAGGTGGAAA TGATTCAGAT GAATTAGCAA ATGGAGAAGT GGGAGGTGAC 
CGGAATGAGA AGATGAAAAA TCAAATTAAG CAGATGATGG ATGTCTCTCG GACACAGACT 
GCAATATCGG TAGTTGAAGA GGATCTAAAG CTTTTACAGC TTAAGCTAAG AGCCTCCGTG 
TCCACTAAAT GTAACCTGGA AGACCAGGTA AAGAAATTGG AAGATGACCG CAACTCACTA 
CAAGCTGCCA AAGCTGGACT GGAAGATGAA TGCAAAACCT TGAGGCAGAA AGTGGAGATT 
CTGAATGAGC TCTATCAGCA GAAGGAGATG GCTTTGCAAA AGAAACTGAG TCAAGAAGAG 
TATGAACGGC AAGAAAGAGA GCACAGGCTG TCAGCTGCAG ATGAAAAGGC AGTTTCGGCT 
GCAGAGGAAG TAAAAACTTA CAAGCGGAGA ATTGAAGAAA TGGAGGATGA ATTACAGAAG 
ACAGAGCGGT CATTTAAAAA CCAGATCGCT ACCCATGAGA AGAAAGCTCA TGAAAACTGG 
CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCTGAAGAGA AAAGGGAAGC TGCCAATTTG 
AGACACAAAT TATTAGAATT AACACAAAAG ATGGCAATGC TGCAAGAAGA ACCTGTGATT 
GTAAAACCAA TGCCAGGAAA ACCAAATACA CAAAACCCTC CACGGAGAGG TCCTCTGAGC 
CAGAATGGCT CTTTTGGCCC ATCCCCTGTG AGTGGTGGAG AATGCTCCCC TCCATTGACA 
GTGGAGCCAC CCGTGAGACC TCTCTCTGCT ACTCTCAATC GAAGAGATAT GCCTAGAAGT 
GAATTTGGAT CAGTGGACGG GCCTCTACCT CATCCTCGAT GGTCAGCTGA GGCATCTGGG 
AAACCCTCTC CTTCTGATCC AGGATCTGGT ACAGCTACCA TGATGAACAG CAGCTCAAGA 
GGCTCTTCCC CTACCAGGGT ACTCGATGAA GGCAAGCAAA CTGTTCTCCA AGAGCCTGAA 
GTCCCCTCAG TTCCCAGCAT TACATCTTTG GCTGAGCGTC CAGTAGCAGT TAATATGGCT 
CCAAAAGGGC CCCCTCCTTT CCCAGGAGTC CCTCTCATGA GCACCCCCAT GGGAGGCCCT 
GTACCACCAC CCATTCGATA TGGACCACCA CCTCAGCTCT GCGGACCTTT TGGGCCTCGG 
CCACTTCCTC CACCCTTTGG CCCTGGTATG CGTCCACCAC TAGGCTTAAG AGAATTTGCA 
CCAGGCGTTC CACCAGGAAG ACGGGACCTG CCTCTCCACC CTCGGGGATT TTTACCTGGA 
CACGCACCAT TTAGACCTTT AGGTTCACTT GGCCCAAGAG AGTACTTTAT TCCTGGTACC 
CGATTACCAC CCCCAACCCA TGGTCCCCAG GAATACCCAC CACCACCTGC TGTAAGAGAC 
TTACTGCCGT CAGGCTCTAG AGATGAGCCT CCACCTGCCT CTCAGAGCAC TAGCCAGGAC 
TGTTCACAGG CTTTAAAACA GAGCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310994.1
CDS	174..2603
Translation	MDSVPATVPSIAATPGDPELVGPLSVLYAAFIAKLLELVATLPDDVQPGPDFYGLPWKPVFITAFLGIASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLISENKKSIEKLKDVISMNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREHRLSAADEKAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGKPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKQTVLQEPEVPSVPSITSLAERPVAVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

Target ORF information:

RefSeq Version	NM_001324065.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324065.1

ORF Insert Sequence:

ATGGACTCAG TACCTGCCAC TGTGCCTTCT ATCGCCGCTA CCCCGGGGGA CCCGGAACTT 
GTGGGACCCT TGTCTGTGCT CTACGCAGCC TTCATAGCCA AGCTGCTGGA GCTAGTTGCT 
ACATTGCCTG ATGATGTTCA GCCTGGGCCT GATTTTTATG GACTGCCATG GAAACCTGTA 
TTTATCACTG CCTTCTTGGG AATTGCTTCG TTTGCCATTT TCTTATGGAG AACTGTCCTT 
GTTGTGAAGG ATAGAGTATA TCAAGTCACG GAACAGCAAA TTTCTGAGAA GTTGAAGACT 
ATCATGAAAG AAAATACAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATCAAG 
GAATCAAAGA AACATGTTCA GGAAACCAGG AAACAAAATA TGATTCTCTC TGATGAAGCA 
ATTAAATATA AGGATAAAAT CAAGACACTT GAAAAAAATC AGGAAATTCT GGATGACACA 
GCTAAAAATC TTCGTGTTAT GCTAGAATCT GAGAGAGAAC AGAATGTCAA GAATCAGGAC 
TTGATATCAG AAAACAAGAA ATCTATAGAG AAGTTAAAGG ATGTTATTTC AATGAATGCC 
TCAGAATTTT CAGAGGTTCA GATTGCACTT AATGAAGCTA AGCTTAGTGA AGAGAAGGTG 
AAGTCTGAAT GCCATCGGGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA AAAAGAGCAG 
TTGCAGCAGG AAATCGAAGA CTGGAGTAAA TTACATGCTG AGCTCAGTGA GCAAATCAAA 
TCATTTGAGA AGTCTCAGAA AGATTTGGAA GTAGCTCTTA CTCACAAGGA TGATAATATT 
AATGCTTTGA CTAACTGCAT TACACAGTTG AATCTGTTAG AGTGTGAATC TGAATCTGAG 
GGTCAAAATA AAGGTGGAAA TGATTCAGAT GAATTAGCAA ATGGAGAAGT GGGAGGTGAC 
CGGAATGAGA AGATGAAAAA TCAAATTAAG CAGATGATGG ATGTCTCTCG GACACAGACT 
GCAATATCGG TAGTTGAAGA GGATCTAAAG CTTTTACAGC TTAAGCTAAG AGCCTCCGTG 
TCCACTAAAT GTAACCTGGA AGACCAGGTA AAGAAATTGG AAGATGACCG CAACTCACTA 
CAAGCTGCCA AAGCTGGACT GGAAGATGAA TGCAAAACCT TGAGGCAGAA AGTGGAGATT 
CTGAATGAGC TCTATCAGCA GAAGGAGATG GCTTTGCAAA AGAAACTGAG TCAAGAAGAG 
TATGAACGGC AAGAAAGAGA GCACAGGCTG TCAGCTGCAG ATGAAAAGGC AGTTTCGGCT 
GCAGAGGAAG TAAAAACTTA CAAGCGGAGA ATTGAAGAAA TGGAGGATGA ATTACAGAAG 
ACAGAGCGGT CATTTAAAAA CCAGATCGCT ACCCATGAGA AGAAAGCTCA TGAAAACTGG 
CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCTGAAGAGA AAAGGGAAGC TGCCAATTTG 
AGACACAAAT TATTAGAATT AACACAAAAG ATGGCAATGC TGCAAGAAGA ACCTGTGATT 
GTAAAACCAA TGCCAGGAAA ACCAAATACA CAAAACCCTC CACGGAGAGG TCCTCTGAGC 
CAGAATGGCT CTTTTGGCCC ATCCCCTGTG AGTGGTGGAG AATGCTCCCC TCCATTGACA 
GTGGAGCCAC CCGTGAGACC TCTCTCTGCT ACTCTCAATC GAAGAGATAT GCCTAGAAGT 
GAATTTGGAT CAGTGGACGG GCCTCTACCT CATCCTCGAT GGTCAGCTGA GGCATCTGGG 
AAACCCTCTC CTTCTGATCC AGGATCTGGT ACAGCTACCA TGATGAACAG CAGCTCAAGA 
GGCTCTTCCC CTACCAGGGT ACTCGATGAA GGCAAGCAAA CTGTTCTCCA AGAGCCTGAA 
GTCCCCTCAG TTCCCAGCAT TACATCTTTG GCTGAGCGTC CAGTAGCAGT TAATATGGCT 
CCAAAAGGGC CCCCTCCTTT CCCAGGAGTC CCTCTCATGA GCACCCCCAT GGGAGGCCCT 
GTACCACCAC CCATTCGATA TGGACCACCA CCTCAGCTCT GCGGACCTTT TGGGCCTCGG 
CCACTTCCTC CACCCTTTGG CCCTGGTATG CGTCCACCAC TAGGCTTAAG AGAATTTGCA 
CCAGGCGTTC CACCAGGAAG ACGGGACCTG CCTCTCCACC CTCGGGGATT TTTACCTGGA 
CACGCACCAT TTAGACCTTT AGGTTCACTT GGCCCAAGAG AGTACTTTAT TCCTGGTACC 
CGATTACCAC CCCCAACCCA TGGTCCCCAG GAATACCCAC CACCACCTGC TGTAAGAGAC 
TTACTGCCGT CAGGCTCTAG AGATGAGCCT CCACCTGCCT CTCAGAGCAC TAGCCAGGAC 
TGTTCACAGG CTTTAAAACA GAGCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( NM_001324064.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310993.1 Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu100260
Clone ID Related Accession (Same CDS sequence)	NM_001324064.1
Accession Version	NM_001324064.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	transport and Golgi organization protein 1 homolog isoform 5
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592148.7 and KF459535.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.158883.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAAAA ACCCTGAATT ATCTAAGGAA AGGGAACCTG AACCTGAACC AGTAGAAGCC 
AACTCAGAGG AAAGTGATAG TGTATTCTCA GAAAACACTG AGGATCTTCA GGAACAGTTT 
ACAACTCAGA AGCACCACTC CCATGCAAAC AGCCAAGCAA ATCATGCTCA GGGAGAGCAG 
GCTTCATTTG AATCTTTTGA AGAAATGCTG CAAGATAAAC TAAAAGTGCC AGAAAGTGAA 
AACAACAAAA CCAGCAATAG TTCTCAGGTC TCAAATGAAC AGGATAAGAT TGATGCCTAT 
AAACTTTTGA AAAAAGAAAT GACTCTAGAC TTGAAAACCA AATTTGGCTC AACAGCTGAT 
GCACTTGTAT CTGATGATGA GACAACCAGA CTCGTTACTT CATTAGAAGA TGATTTTGAT 
GAGGAATTGG ATACTGAGTA TTATGCAGTT GGAAAGGAAG ATGAGGAGAA CCAAGAAGAC 
TTTGATGAGT TGCCATTACT TACCTTTACA GATGGGGAAG ATATGAAAAC TCCAGCAAAG 
TCTGGCGTTG AGAAATATCC AACAGATAAA GAGCAGAATT CAAATGAAGA GGACAAGGTT 
CAGCTAACTG TGCCCCCTGG CATCAAAAAT GATGATAAAA ATATACTAAC AACCTGGGGG 
GACACTATCT TCTCTATTGT CACAGGAGGT GAAGAAACAA GAGATACGAT GGATTTAGAG 
AGCTCTAGTT CAGAGGAAGA AAAAGAAGAT GATGATGATG CATTAGTCCC AGATAGCAAA 
CAGGGGAAAC CACAGTCAGC AACAGATTAT AGTGACCCTG ACAATGTAGA TGATGGTCTT 
TTTATTGTAG ACATTCCTAA AACAAATAAT GACAAAGAAG TAAACGCAGA ACATCACATT 
AAAGGAAAAG GGAGGGGAGT TCAGGAATCC AAGAGGGGCC TGGTACAAGA TAAGACAGAA 
TTAGAGGATG AAAATCAAGA AGGCATGACT GTGCACAGTT CTGTTCACAG CAATAACCTC 
AACTCTATGC CAGCTGCTGA AAAGGGTAAA GACACATTAA AATCAGCTTA TGATGATACA 
GAAAATGACC TAAAAGGAGC AGCTATTCAT ATCTCAAAAG GAATGCTCCA CGAAGAAAAG 
CCTGGAGAGC AGATTTTGGA AGGTGGCTCA GAGAGTGAAT CTGCACAGAA AGCTGCAGGG 
AATCAAATGA ATGACAGAAA GATTCAACAG GAATCCCTGG GTAGTGCACC ACTCATGGGA 
GATGACCACC CTAACGCATC CAGAGACAGT GTGGAGGGAG ACGCTTTGGT AAATGGGGCC 
AAACTGCACA CGCTTTCAGT GGAGCATCAA CGTGAGGAAT TGAAAGAGGA ATTAGTTCTT 
AAAACTCAAA ACCAACCTAG ATTCTCCTCT CCAGATGAGA TTGATTTGCC CAGAGAACTG 
GAAGACGAGG TTCCCATTCT GGGAAGAAAT CTTCCCTGGC AACAAGAAAG AGATGTGGCT 
GCCACAGCCA GTAAGCAAAT GAGTGAGAAG ATAAGGCTCT CTGAGGGAGA AGCCAAAGAG 
GACTCCTTGG ATGAAGAGTT TTTTCATCAC AAGGCAATGC AGGGCACAGA GGTAGGACAG 
ACAGACCAAA CTGACAGCAC AGGAGGACCA GCTTTCCTTT CTAAAGTAGA AGAGGATGAT 
TATCCCTCTG AAGAACTACT AGAGGATGAA AACGCTATAA ATGCAAAACG GTCTAAAGAA 
AAAAACCCTG GGAATCAGGG CAGGCAGTTT GATGTTAATC TGCAAGTCCC TGACAGAGCA 
GTTTTAGGGA CCATTCATCC AGATCCAGAA ATTGAAGAAA GCAAGCAAGA AACTAGTATG 
ATTTTGGATA GCGAAAAAAC AAGTGAGACT GCTGCCAAAG GGGTCAACAC AGGAGGCAGG 
GAACCAAATA CAATGGTGGA AAAAGAACGC CCTCTGGCAG ATAAGAAAGC ACAGAGACCA 
TTTGAACGAA GTGACTTTTC TGACAGCATA AAAATTCAGA CTCCAGAATT AGGTGAAGTG 
TTTCAGAATA AAGATTCTGA TTATCTGAAG AACGACAACC CTGAGGAACA TCTGAAGACC 
TCAGGGCTTG CAGGGGAGCC TGAGGGAGAA CTCTCAAAAG AGGACCATGA GAACACAGAG 
AAGTACATGG GCACAGAAAG CCAGGGGTCT GCTGCTGCAG AACCTGAAGA TGACTCGTTC 
CACTGGACTC CACATACAAG TGTAGAGCCA GGGCATAGTG ACAAGAGGGA GGACTTACTT 
ATCATAAGCA GCTTCTTTAA AGAACAACAG TCTTTGCAGC GGTTCCAGAA GTACTTTAAT 
GTCCATGAGC TGGAAGCCTT GCTACAAGAA ATGTCATCAA AACTGAAGTC AGCGCAGCAG 
GAGAGCCTGC CCTATAATAT GGAAAAAGTC CTAGATAAGG TCTTCCGTGC TTCTGAGTCA 
CAAATTCTGA GCATAGCAGA AAAAATGCTT GATACTCGTG TGGCTGAAAA TAGAGATCTG 
GGAATGAACG AAAATAACAT ATTTGAAGAG GCTGCAGTGC TTGATGACAT TCAAGACCTC 
ATCTATTTTG TCAGGTACAA GCACTCCACA GCAGAGGAGA CAGCCACACT GGTGATGGCA 
CCACCTCTAG AGGAAGGCTT GGGTGGAGCA ATGGAAGAGA TGCAACCACT GCATGAAGAT 
AATTTCTCAC GAGAGAAGAC AGCAGAACTT AATGTGCAGG TTCCTGAAGA ACCCACCCAC 
TTGGACCAAC GTGTGATTGG GGACACTCAT GCCTCAGAAG TGTCACAGAA GCCAAATACT 
GAGAAAGACC TGGACCCAGG GCCAGTTACA ACAGAAGACA CTCCTATGGA TGCTATTGAT 
GCAAACAAGC AACCAGAGAC AGCCGCCGAA GAGCCGGCAA GTGTCACACC TTTGGAAAAC 
GCAATCCTTC TAATATATTC ATTCATGTTT TATTTAACTA AGTCGCTAGT TGCTACATTG 
CCTGATGATG TTCAGCCTGG GCCTGATTTT TATGGACTGC CATGGAAACC TGTATTTATC 
ACTGCCTTCT TGGGAATTGC TTCGTTTGCC ATTTTCTTAT GGAGAACTGT CCTTGTTGTG 
AAGGATAGAG TATATCAAGT CACGGAACAG CAAATTTCTG AGAAGTTGAA GACTATCATG 
AAAGAAAATA CAGAACTTGT ACAAAAATTG TCAAATTATG AACAGAAGAT CAAGGAATCA 
AAGAAACATG TTCAGGAAAC CAGGAAACAA AATATGATTC TCTCTGATGA AGCAATTAAA 
TATAAGGATA AAATCAAGAC ACTTGAAAAA AATCAGGAAA TTCTGGATGA CACAGCTAAA 
AATCTTCGTG TTATGCTAGA ATCTGAGAGA GAACAGAATG TCAAGAATCA GGACTTGATA 
TCAGAAAACA AGAAATCTAT AGAGAAGTTA AAGGATGTTA TTTCAATGAA TGCCTCAGAA 
TTTTCAGAGG TTCAGATTGC ACTTAATGAA GCTAAGCTTA GTGAAGAGAA GGTGAAGTCT 
GAATGCCATC GGGTTCAAGA AGAAAATGCT AGGCTTAAGA AGAAAAAAGA GCAGTTGCAG 
CAGGAAATCG AAGACTGGAG TAAATTACAT GCTGAGCTCA GTGAGCAAAT CAAATCATTT 
GAGAAGTCTC AGAAAGATTT GGAAGTAGCT CTTACTCACA AGGATGATAA TATTAATGCT 
TTGACTAACT GCATTACACA GTTGAATCTG TTAGAGTGTG AATCTGAATC TGAGGGTCAA 
AATAAAGGTG GAAATGATTC AGATGAATTA GCAAATGGAG AAGTGGGAGG TGACCGGAAT 
GAGAAGATGA AAAATCAAAT TAAGCAGATG ATGGATGTCT CTCGGACACA GACTGCAATA 
TCGGTAGTTG AAGAGGATCT AAAGCTTTTA CAGCTTAAGC TAAGAGCCTC CGTGTCCACT 
AAATGTAACC TGGAAGACCA GGTAAAGAAA TTGGAAGATG ACCGCAACTC ACTACAAGCT 
GCCAAAGCTG GACTGGAAGA TGAATGCAAA ACCTTGAGGC AGAAAGTGGA GATTCTGAAT 
GAGCTCTATC AGCAGAAGGA GATGGCTTTG CAAAAGAAAC TGAGTCAAGA AGAGTATGAA 
CGGCAAGAAA GAGAGCACAG GCTGTCAGCT GCAGATGAAA AGGCAGTTTC GGCTGCAGAG 
GAAGTAAAAA CTTACAAGCG GAGAATTGAA GAAATGGAGG ATGAATTACA GAAGACAGAG 
CGGTCATTTA AAAACCAGAT CGCTACCCAT GAGAAGAAAG CTCATGAAAA CTGGCTCAAA 
GCTCGTGCTG CAGAAAGAGC TATAGCTGAA GAGAAAAGGG AAGCTGCCAA TTTGAGACAC 
AAATTATTAG AATTAACACA AAAGATGGCA ATGCTGCAAG AAGAACCTGT GATTGTAAAA 
CCAATGCCAG GAAAACCAAA TACACAAAAC CCTCCACGGA GAGGTCCTCT GAGCCAGAAT 
GGCTCTTTTG GCCCATCCCC TGTGAGTGGT GGAGAATGCT CCCCTCCATT GACAGTGGAG 
CCACCCGTGA GACCTCTCTC TGCTACTCTC AATCGAAGAG ATATGCCTAG AAGTGAATTT 
GGATCAGTGG ACGGGCCTCT ACCTCATCCT CGATGGTCAG CTGAGGCATC TGGGAAACCC 
TCTCCTTCTG ATCCAGGATC TGGTACAGCT ACCATGATGA ACAGCAGCTC AAGAGGCTCT 
TCCCCTACCA GGGTACTCGA TGAAGGCAAG GTTAATATGG CTCCAAAAGG GCCCCCTCCT 
TTCCCAGGAG TCCCTCTCAT GAGCACCCCC ATGGGAGGCC CTGTACCACC ACCCATTCGA 
TATGGACCAC CACCTCAGCT CTGCGGACCT TTTGGGCCTC GGCCACTTCC TCCACCCTTT 
GGCCCTGGTA TGCGTCCACC ACTAGGCTTA AGAGAATTTG CACCAGGCGT TCCACCAGGA 
AGACGGGACC TGCCTCTCCA CCCTCGGGGA TTTTTACCTG GACACGCACC ATTTAGACCT 
TTAGGTTCAC TTGGCCCAAG AGAGTACTTT ATTCCTGGTA CCCGATTACC ACCCCCAACC 
CATGGTCCCC AGGAATACCC ACCACCACCT GCTGTAAGAG ACTTACTGCC GTCAGGCTCT 
AGAGATGAGC CTCCACCTGC CTCTCAGAGC ACTAGCCAGG ACTGTTCACA GGCTTTAAAA 
CAGAGCCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310993.1
CDS	547..5778
Translation	MEKNPELSKEREPEPEPVEANSEESDSVFSENTEDLQEQFTTQKHHSHANSQANHAQGEQASFESFEEMLQDKLKVPESENNKTSNSSQVSNEQDKIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDTEYYAVGKEDEENQEDFDELPLLTFTDGEDMKTPAKSGVEKYPTDKEQNSNEEDKVQLTVPPGIKNDDKNILTTWGDTIFSIVTGGEETRDTMDLESSSSEEEKEDDDDALVPDSKQGKPQSATDYSDPDNVDDGLFIVDIPKTNNDKEVNAEHHIKGKGRGVQESKRGLVQDKTELEDENQEGMTVHSSVHSNNLNSMPAAEKGKDTLKSAYDDTENDLKGAAIHISKGMLHEEKPGEQILEGGSESESAQKAAGNQMNDRKIQQESLGSAPLMGDDHPNASRDSVEGDALVNGAKLHTLSVEHQREELKEELVLKTQNQPRFSSPDEIDLPRELEDEVPILGRNLPWQQERDVAATASKQMSEKIRLSEGEAKEDSLDEEFFHHKAMQGTEVGQTDQTDSTGGPAFLSKVEEDDYPSEELLEDENAINAKRSKEKNPGNQGRQFDVNLQVPDRAVLGTIHPDPEIEESKQETSMILDSEKTSETAAKGVNTGGREPNTMVEKERPLADKKAQRPFERSDFSDSIKIQTPELGEVFQNKDSDYLKNDNPEEHLKTSGLAGEPEGELSKEDHENTEKYMGTESQGSAAAEPEDDSFHWTPHTSVEPGHSDKREDLLIISSFFKEQQSLQRFQKYFNVHELEALLQEMSSKLKSAQQESLPYNMEKVLDKVFRASESQILSIAEKMLDTRVAENRDLGMNENNIFEEAAVLDDIQDLIYFVRYKHSTAEETATLVMAPPLEEGLGGAMEEMQPLHEDNFSREKTAELNVQVPEEPTHLDQRVIGDTHASEVSQKPNTEKDLDPGPVTTEDTPMDAIDANKQPETAAEEPASVTPLENAILLIYSFMFYLTKSLVATLPDDVQPGPDFYGLPWKPVFITAFLGIASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLISENKKSIEKLKDVISMNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREHRLSAADEKAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGKPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

Target ORF information:

RefSeq Version	NM_001324064.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324064.1

ORF Insert Sequence:

ATGGAAAAAA ACCCTGAATT ATCTAAGGAA AGGGAACCTG AACCTGAACC AGTAGAAGCC 
AACTCAGAGG AAAGTGATAG TGTATTCTCA GAAAACACTG AGGATCTTCA GGAACAGTTT 
ACAACTCAGA AGCACCACTC CCATGCAAAC AGCCAAGCAA ATCATGCTCA GGGAGAGCAG 
GCTTCATTTG AATCTTTTGA AGAAATGCTG CAAGATAAAC TAAAAGTGCC AGAAAGTGAA 
AACAACAAAA CCAGCAATAG TTCTCAGGTC TCAAATGAAC AGGATAAGAT TGATGCCTAT 
AAACTTTTGA AAAAAGAAAT GACTCTAGAC TTGAAAACCA AATTTGGCTC AACAGCTGAT 
GCACTTGTAT CTGATGATGA GACAACCAGA CTCGTTACTT CATTAGAAGA TGATTTTGAT 
GAGGAATTGG ATACTGAGTA TTATGCAGTT GGAAAGGAAG ATGAGGAGAA CCAAGAAGAC 
TTTGATGAGT TGCCATTACT TACCTTTACA GATGGGGAAG ATATGAAAAC TCCAGCAAAG 
TCTGGCGTTG AGAAATATCC AACAGATAAA GAGCAGAATT CAAATGAAGA GGACAAGGTT 
CAGCTAACTG TGCCCCCTGG CATCAAAAAT GATGATAAAA ATATACTAAC AACCTGGGGG 
GACACTATCT TCTCTATTGT CACAGGAGGT GAAGAAACAA GAGATACGAT GGATTTAGAG 
AGCTCTAGTT CAGAGGAAGA AAAAGAAGAT GATGATGATG CATTAGTCCC AGATAGCAAA 
CAGGGGAAAC CACAGTCAGC AACAGATTAT AGTGACCCTG ACAATGTAGA TGATGGTCTT 
TTTATTGTAG ACATTCCTAA AACAAATAAT GACAAAGAAG TAAACGCAGA ACATCACATT 
AAAGGAAAAG GGAGGGGAGT TCAGGAATCC AAGAGGGGCC TGGTACAAGA TAAGACAGAA 
TTAGAGGATG AAAATCAAGA AGGCATGACT GTGCACAGTT CTGTTCACAG CAATAACCTC 
AACTCTATGC CAGCTGCTGA AAAGGGTAAA GACACATTAA AATCAGCTTA TGATGATACA 
GAAAATGACC TAAAAGGAGC AGCTATTCAT ATCTCAAAAG GAATGCTCCA CGAAGAAAAG 
CCTGGAGAGC AGATTTTGGA AGGTGGCTCA GAGAGTGAAT CTGCACAGAA AGCTGCAGGG 
AATCAAATGA ATGACAGAAA GATTCAACAG GAATCCCTGG GTAGTGCACC ACTCATGGGA 
GATGACCACC CTAACGCATC CAGAGACAGT GTGGAGGGAG ACGCTTTGGT AAATGGGGCC 
AAACTGCACA CGCTTTCAGT GGAGCATCAA CGTGAGGAAT TGAAAGAGGA ATTAGTTCTT 
AAAACTCAAA ACCAACCTAG ATTCTCCTCT CCAGATGAGA TTGATTTGCC CAGAGAACTG 
GAAGACGAGG TTCCCATTCT GGGAAGAAAT CTTCCCTGGC AACAAGAAAG AGATGTGGCT 
GCCACAGCCA GTAAGCAAAT GAGTGAGAAG ATAAGGCTCT CTGAGGGAGA AGCCAAAGAG 
GACTCCTTGG ATGAAGAGTT TTTTCATCAC AAGGCAATGC AGGGCACAGA GGTAGGACAG 
ACAGACCAAA CTGACAGCAC AGGAGGACCA GCTTTCCTTT CTAAAGTAGA AGAGGATGAT 
TATCCCTCTG AAGAACTACT AGAGGATGAA AACGCTATAA ATGCAAAACG GTCTAAAGAA 
AAAAACCCTG GGAATCAGGG CAGGCAGTTT GATGTTAATC TGCAAGTCCC TGACAGAGCA 
GTTTTAGGGA CCATTCATCC AGATCCAGAA ATTGAAGAAA GCAAGCAAGA AACTAGTATG 
ATTTTGGATA GCGAAAAAAC AAGTGAGACT GCTGCCAAAG GGGTCAACAC AGGAGGCAGG 
GAACCAAATA CAATGGTGGA AAAAGAACGC CCTCTGGCAG ATAAGAAAGC ACAGAGACCA 
TTTGAACGAA GTGACTTTTC TGACAGCATA AAAATTCAGA CTCCAGAATT AGGTGAAGTG 
TTTCAGAATA AAGATTCTGA TTATCTGAAG AACGACAACC CTGAGGAACA TCTGAAGACC 
TCAGGGCTTG CAGGGGAGCC TGAGGGAGAA CTCTCAAAAG AGGACCATGA GAACACAGAG 
AAGTACATGG GCACAGAAAG CCAGGGGTCT GCTGCTGCAG AACCTGAAGA TGACTCGTTC 
CACTGGACTC CACATACAAG TGTAGAGCCA GGGCATAGTG ACAAGAGGGA GGACTTACTT 
ATCATAAGCA GCTTCTTTAA AGAACAACAG TCTTTGCAGC GGTTCCAGAA GTACTTTAAT 
GTCCATGAGC TGGAAGCCTT GCTACAAGAA ATGTCATCAA AACTGAAGTC AGCGCAGCAG 
GAGAGCCTGC CCTATAATAT GGAAAAAGTC CTAGATAAGG TCTTCCGTGC TTCTGAGTCA 
CAAATTCTGA GCATAGCAGA AAAAATGCTT GATACTCGTG TGGCTGAAAA TAGAGATCTG 
GGAATGAACG AAAATAACAT ATTTGAAGAG GCTGCAGTGC TTGATGACAT TCAAGACCTC 
ATCTATTTTG TCAGGTACAA GCACTCCACA GCAGAGGAGA CAGCCACACT GGTGATGGCA 
CCACCTCTAG AGGAAGGCTT GGGTGGAGCA ATGGAAGAGA TGCAACCACT GCATGAAGAT 
AATTTCTCAC GAGAGAAGAC AGCAGAACTT AATGTGCAGG TTCCTGAAGA ACCCACCCAC 
TTGGACCAAC GTGTGATTGG GGACACTCAT GCCTCAGAAG TGTCACAGAA GCCAAATACT 
GAGAAAGACC TGGACCCAGG GCCAGTTACA ACAGAAGACA CTCCTATGGA TGCTATTGAT 
GCAAACAAGC AACCAGAGAC AGCCGCCGAA GAGCCGGCAA GTGTCACACC TTTGGAAAAC 
GCAATCCTTC TAATATATTC ATTCATGTTT TATTTAACTA AGTCGCTAGT TGCTACATTG 
CCTGATGATG TTCAGCCTGG GCCTGATTTT TATGGACTGC CATGGAAACC TGTATTTATC 
ACTGCCTTCT TGGGAATTGC TTCGTTTGCC ATTTTCTTAT GGAGAACTGT CCTTGTTGTG 
AAGGATAGAG TATATCAAGT CACGGAACAG CAAATTTCTG AGAAGTTGAA GACTATCATG 
AAAGAAAATA CAGAACTTGT ACAAAAATTG TCAAATTATG AACAGAAGAT CAAGGAATCA 
AAGAAACATG TTCAGGAAAC CAGGAAACAA AATATGATTC TCTCTGATGA AGCAATTAAA 
TATAAGGATA AAATCAAGAC ACTTGAAAAA AATCAGGAAA TTCTGGATGA CACAGCTAAA 
AATCTTCGTG TTATGCTAGA ATCTGAGAGA GAACAGAATG TCAAGAATCA GGACTTGATA 
TCAGAAAACA AGAAATCTAT AGAGAAGTTA AAGGATGTTA TTTCAATGAA TGCCTCAGAA 
TTTTCAGAGG TTCAGATTGC ACTTAATGAA GCTAAGCTTA GTGAAGAGAA GGTGAAGTCT 
GAATGCCATC GGGTTCAAGA AGAAAATGCT AGGCTTAAGA AGAAAAAAGA GCAGTTGCAG 
CAGGAAATCG AAGACTGGAG TAAATTACAT GCTGAGCTCA GTGAGCAAAT CAAATCATTT 
GAGAAGTCTC AGAAAGATTT GGAAGTAGCT CTTACTCACA AGGATGATAA TATTAATGCT 
TTGACTAACT GCATTACACA GTTGAATCTG TTAGAGTGTG AATCTGAATC TGAGGGTCAA 
AATAAAGGTG GAAATGATTC AGATGAATTA GCAAATGGAG AAGTGGGAGG TGACCGGAAT 
GAGAAGATGA AAAATCAAAT TAAGCAGATG ATGGATGTCT CTCGGACACA GACTGCAATA 
TCGGTAGTTG AAGAGGATCT AAAGCTTTTA CAGCTTAAGC TAAGAGCCTC CGTGTCCACT 
AAATGTAACC TGGAAGACCA GGTAAAGAAA TTGGAAGATG ACCGCAACTC ACTACAAGCT 
GCCAAAGCTG GACTGGAAGA TGAATGCAAA ACCTTGAGGC AGAAAGTGGA GATTCTGAAT 
GAGCTCTATC AGCAGAAGGA GATGGCTTTG CAAAAGAAAC TGAGTCAAGA AGAGTATGAA 
CGGCAAGAAA GAGAGCACAG GCTGTCAGCT GCAGATGAAA AGGCAGTTTC GGCTGCAGAG 
GAAGTAAAAA CTTACAAGCG GAGAATTGAA GAAATGGAGG ATGAATTACA GAAGACAGAG 
CGGTCATTTA AAAACCAGAT CGCTACCCAT GAGAAGAAAG CTCATGAAAA CTGGCTCAAA 
GCTCGTGCTG CAGAAAGAGC TATAGCTGAA GAGAAAAGGG AAGCTGCCAA TTTGAGACAC 
AAATTATTAG AATTAACACA AAAGATGGCA ATGCTGCAAG AAGAACCTGT GATTGTAAAA 
CCAATGCCAG GAAAACCAAA TACACAAAAC CCTCCACGGA GAGGTCCTCT GAGCCAGAAT 
GGCTCTTTTG GCCCATCCCC TGTGAGTGGT GGAGAATGCT CCCCTCCATT GACAGTGGAG 
CCACCCGTGA GACCTCTCTC TGCTACTCTC AATCGAAGAG ATATGCCTAG AAGTGAATTT 
GGATCAGTGG ACGGGCCTCT ACCTCATCCT CGATGGTCAG CTGAGGCATC TGGGAAACCC 
TCTCCTTCTG ATCCAGGATC TGGTACAGCT ACCATGATGA ACAGCAGCTC AAGAGGCTCT 
TCCCCTACCA GGGTACTCGA TGAAGGCAAG GTTAATATGG CTCCAAAAGG GCCCCCTCCT 
TTCCCAGGAG TCCCTCTCAT GAGCACCCCC ATGGGAGGCC CTGTACCACC ACCCATTCGA 
TATGGACCAC CACCTCAGCT CTGCGGACCT TTTGGGCCTC GGCCACTTCC TCCACCCTTT 
GGCCCTGGTA TGCGTCCACC ACTAGGCTTA AGAGAATTTG CACCAGGCGT TCCACCAGGA 
AGACGGGACC TGCCTCTCCA CCCTCGGGGA TTTTTACCTG GACACGCACC ATTTAGACCT 
TTAGGTTCAC TTGGCCCAAG AGAGTACTTT ATTCCTGGTA CCCGATTACC ACCCCCAACC 
CATGGTCCCC AGGAATACCC ACCACCACCT GCTGTAAGAG ACTTACTGCC GTCAGGCTCT 
AGAGATGAGC CTCCACCTGC CTCTCAGAGC ACTAGCCAGG ACTGTTCACA GGCTTTAAAA 
CAGAGCCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( NM_001324063.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310992.1 Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu100264
Clone ID Related Accession (Same CDS sequence)	NM_001324063.1
Accession Version	NM_001324063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	transport and Golgi organization protein 1 homolog isoform 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592148.7 and KF459535.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.814.1, SRR1803617.252802.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGG CGCCTGGGCT GCTCGTCTGG CTGCTCGTGC TCCGGCTGCC CTGGCGGGTG 
CCGGGCCAGC TGGACCCCAG CACTGGCCGG CGGTTCTCGG AGCACAAACT CTGCGCGGAC 
GACGAATGCA GCATGTTAAT GTACCGCGGT GAGGCTCTTG AAGATTTCAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGTGAT CCTGTATATG TTTACTATAA ACTGGCAAGA 
GGATGGCCTG AAGTTTGGGC TGGAAGTGTT GGACGCACTT TTGGATATTT TCCAAAAGAT 
TTAATCCAGG TAGTTCATGA ATATACCAAA GAAGAGCTAC AAGTTCCAAC AGATGAGACG 
GATTTTGTTT GTTTTGATGG AGGAAGAGAT GATTTTCATA ATTATAATGT AGAAGAACTT 
TTAGGGTTTT TGGAACTGTA CAATTCTGCA GCTACAGATT CTGAGAAAGC TGTAGAAAAA 
ACTTTACAGG ATATGGAAAA AAACCCTGAA TTATCTAAGG AAAGGGAACC TGAACCTGAA 
CCAGTAGAAG CCAACTCAGA GGAAAGTGAT AGTGTATTCT CAGAAAACAC TGAGGATCTT 
CAGGAACAGT TTACAACTCA GAAGCACCAC TCCCATGCAA ACAGCCAAGC AAATCATGCT 
CAGGGAGAGC AGGCTTCATT TGAATCTTTT GAAGAAATGC TGCAAGATAA ACTAAAAGTG 
CCAGAAAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCAAATGA ACAGGATAAG 
ATTGATGCCT ATAAACTTTT GAAAAAAGAA ATGACTCTAG ACTTGAAAAC CAAATTTGGC 
TCAACAGCTG ATGCACTTGT ATCTGATGAT GAGACAACCA GACTCGTTAC TTCATTAGAA 
GATGATTTTG ATGAGGAATT GGATACTGAG TATTATGCAG TTGGAAAGGA AGATGAGGAG 
AACCAAGAAG ACTTTGATGA GTTGCCATTA CTTACCTTTA CAGATGGGGA AGATATGAAA 
ACTCCAGCAA AGTCTGGCGT TGAGAAATAT CCAACAGATA AAGAGCAGAA TTCAAATGAA 
GAGGACAAGG TTCAGCTAAC TGTGCCCCCT GGCATCAAAA ATGATGATAA AAATATACTA 
ACAACCTGGG GGGACACTAT CTTCTCTATT GTCACAGGAG GTGAAGAAAC AAGAGATACG 
ATGGATTTAG AGAGCTCTAG TTCAGAGGAA GAAAAAGAAG ATGATGATGA TGCATTAGTC 
CCAGATAGCA AACAGGGGAA ACCACAGTCA GCAACAGATT ATAGTGACCC TGACAATGTA 
GATGATGGTC TTTTTATTGT AGACATTCCT AAAACAAATA ATGACAAAGA AGTAAACGCA 
GAACATCACA TTAAAGGAAA AGGGAGGGGA GTTCAGGAAT CCAAGAGGGG CCTGGTACAA 
GATAAGACAG AATTAGAGGA TGAAAATCAA GAAGGCATGA CTGTGCACAG TTCTGTTCAC 
AGCAATAACC TCAACTCTAT GCCAGCTGCT GAAAAGGGTA AAGACACATT AAAATCAGCT 
TATGATGATA CAGAAAATGA CCTAAAAGGA GCAGCTATTC ATATCTCAAA AGGAATGCTC 
CACGAAGAAA AGCCTGGAGA GCAGATTTTG GAAGGTGGCT CAGAGAGTGA ATCTGCACAG 
AAAGCTGCAG GGAATCAAAT GAATGACAGA AAGATTCAAC AGGAATCCCT GGGTAGTGCA 
CCACTCATGG GAGATGACCA CCCTAACGCA TCCAGAGACA GTGTGGAGGG AGACGCTTTG 
GTAAATGGGG CCAAACTGCA CACGCTTTCA GTGGAGCATC AACGTGAGGA ATTGAAAGAG 
GAATTAGTTC TTAAAACTCA AAACCAACCT AGATTCTCCT CTCCAGATGA GATTGATTTG 
CCCAGAGAAC TGGAAGACGA GGTTCCCATT CTGGGAAGAA ATCTTCCCTG GCAACAAGAA 
AGAGATGTGG CTGCCACAGC CAGTAAGCAA ATGAGTGAGA AGATAAGGCT CTCTGAGGGA 
GAAGCCAAAG AGGACTCCTT GGATGAAGAG TTTTTTCATC ACAAGGCAAT GCAGGGCACA 
GAGGTAGGAC AGACAGACCA AACTGACAGC ACAGGAGGAC CAGCTTTCCT TTCTAAAGTA 
GAAGAGGATG ATTATCCCTC TGAAGAACTA CTAGAGGATG AAAACGCTAT AAATGCAAAA 
CGGTCTAAAG AAAAAAACCC TGGGAATCAG GGCAGGCAGT TTGATGTTAA TCTGCAAGTC 
CCTGACAGAG CAGTTTTAGG GACCATTCAT CCAGATCCAG AAATTGAAGA AAGCAAGCAA 
GAAACTAGTA TGATTTTGGA TAGCGAAAAA ACAAGTGAGA CTGCTGCCAA AGGGGTCAAC 
ACAGGAGGCA GGGAACCAAA TACAATGGTG GAAAAAGAAC GCCCTCTGGC AGATAAGAAA 
GCACAGAGAC CATTTGAACG AAGTGACTTT TCTGACAGCA TAAAAATTCA GACTCCAGAA 
TTAGGTGAAG TGTTTCAGAA TAAAGATTCT GATTATCTGA AGAACGACAA CCCTGAGGAA 
CATCTGAAGA CCTCAGGGCT TGCAGGGGAG CCTGAGGGAG AACTCTCAAA AGAGGACCAT 
GAGAACACAG AGAAGTACAT GGGCACAGAA AGCCAGGGGT CTGCTGCTGC AGAACCTGAA 
GATGACTCGT TCCACTGGAC TCCACATACA AGTGTAGAGC CAGGGCATAG TGACAAGAGG 
GAGGACTTAC TTATCATAAG CAGCTTCTTT AAAGAACAAC AGTCTTTGCA GCGGTTCCAG 
AAGTACTTTA ATGTCCATGA GCTGGAAGCC TTGCTACAAG AAATGTCATC AAAACTGAAG 
TCAGCGCAGC AGGAGAGCCT GCCCTATAAT ATGGAAAAAG TCCTAGATAA GGTCTTCCGT 
GCTTCTGAGT CACAAATTCT GAGCATAGCA GAAAAAATGC TTGATACTCG TGTGGCTGAA 
AATAGAGATC TGGGAATGAA CGAAAATAAC ATATTTGAAG AGGCTGCAGT GCTTGATGAC 
ATTCAAGACC TCATCTATTT TGTCAGGTAC AAGCACTCCA CAGCAGAGGA GACAGCCACA 
CTGGTGATGG CACCACCTCT AGAGGAAGGC TTGGGTGGAG CAATGGAAGA GATGCAACCA 
CTGCATGAAG ATAATTTCTC ACGAGAGAAG ACAGCAGAAC TTAATGTGCA GGTTCCTGAA 
GAACCCACCC ACTTGGACCA ACGTGTGATT GGGGACACTC ATGCCTCAGA AGTGTCACAG 
AAGCCAAATA CTGAGAAAGA CCTGGACCCA GGGCCAGTTA CAACAGAAGA CACTCCTATG 
GATGCTATTG ATGCAAACAA GCAACCAGAG ACAGCCGCCG AAGAGCCGGC AAGTGTCACA 
CCTTTGGAAA ACGCAATCCT TCTAATATAT TCATTCATGT TTTATTTAAC TAAGTCGCTA 
GTTGCTACAT TGCCTGATGA TGTTCAGCCT GGGCCTGATT TTTATGGACT GCCATGGAAA 
CCTGTATTTA TCACTGCCTT CTTGGGAATT GCTTCGTTTG CCATTTTCTT ATGGAGAACT 
GTCCTTGTTG TGAAGGATAG AGTATATCAA GTCACGGAAC AGCAAATTTC TGAGAAGTTG 
AAGACTATCA TGAAAGAAAA TACAGAACTT GTACAAAAAT TGTCAAATTA TGAACAGAAG 
ATCAAGGAAT CAAAGAAACA TGTTCAGGAA ACCAGGAAAC AAAATATGAT TCTCTCTGAT 
GAAGCAATTA AATATAAGGA TAAAATCAAG ACACTTGAAA AAAATCAGGA AATTCTGGAT 
GACACAGCTA AAAATCTTCG TGTTATGCTA GAATCTGAGA GAGAACAGAA TGTCAAGAAT 
CAGGACTTGT TGCAGCAGGA AATCGAAGAC TGGAGTAAAT TACATGCTGA GCTCAGTGAG 
CAAATCAAAT CATTTGAGAA GTCTCAGAAA GATTTGGAAG TAGCTCTTAC TCACAAGGAT 
GATAATATTA ATGCTTTGAC TAACTGCATT ACACAGTTGA ATCTGTTAGA GTGTGAATCT 
GAATCTGAGG GTCAAAATAA AGGTGGAAAT GATTCAGATG AATTAGCAAA TGGAGAAGTG 
GGAGGTGACC GGAATGAGAA GATGAAAAAT CAAATTAAGC AGATGATGGA TGTCTCTCGG 
ACACAGACTG CAATATCGGT AGTTGAAGAG GATCTAAAGC TTTTACAGCT TAAGCTAAGA 
GCCTCCGTGT CCACTAAATG TAACCTGGAA GACCAGGTAA AGAAATTGGA AGATGACCGC 
AACTCACTAC AAGCTGCCAA AGCTGGACTG GAAGATGAAT GCAAAACCTT GAGGCAGAAA 
GTGGAGATTC TGAATGAGCT CTATCAGCAG AAGGAGATGG CTTTGCAAAA GAAACTGAGT 
CAAGAAGAGT ATGAACGGCA AGAAAGAGAG CACAGGCTGT CAGCTGCAGA TGAAAAGGCA 
GTTTCGGCTG CAGAGGAAGT AAAAACTTAC AAGCGGAGAA TTGAAGAAAT GGAGGATGAA 
TTACAGAAGA CAGAGCGGTC ATTTAAAAAC CAGATCGCTA CCCATGAGAA GAAAGCTCAT 
GAAAACTGGC TCAAAGCTCG TGCTGCAGAA AGAGCTATAG CTGAAGAGAA AAGGGAAGCT 
GCCAATTTGA GACACAAATT ATTAGAATTA ACACAAAAGA TGGCAATGCT GCAAGAAGAA 
CCTGTGATTG TAAAACCAAT GCCAGGAAAA CCAAATACAC AAAACCCTCC ACGGAGAGGT 
CCTCTGAGCC AGAATGGCTC TTTTGGCCCA TCCCCTGTGA GTGGTGGAGA ATGCTCCCCT 
CCATTGACAG TGGAGCCACC CGTGAGACCT CTCTCTGCTA CTCTCAATCG AAGAGATATG 
CCTAGAAGTG AATTTGGATC AGTGGACGGG CCTCTACCTC ATCCTCGATG GTCAGCTGAG 
GCATCTGGGA AACCCTCTCC TTCTGATCCA GGATCTGGTA CAGCTACCAT GATGAACAGC 
AGCTCAAGAG GCTCTTCCCC TACCAGGGTA CTCGATGAAG GCAAGGTTAA TATGGCTCCA 
AAAGGGCCCC CTCCTTTCCC AGGAGTCCCT CTCATGAGCA CCCCCATGGG AGGCCCTGTA 
CCACCACCCA TTCGATATGG ACCACCACCT CAGCTCTGCG GACCTTTTGG GCCTCGGCCA 
CTTCCTCCAC CCTTTGGCCC TGGTATGCGT CCACCACTAG GCTTAAGAGA ATTTGCACCA 
GGCGTTCCAC CAGGAAGACG GGACCTGCCT CTCCACCCTC GGGGATTTTT ACCTGGACAC 
GCACCATTTA GACCTTTAGG TTCACTTGGC CCAAGAGAGT ACTTTATTCC TGGTACCCGA 
TTACCACCCC CAACCCATGG TCCCCAGGAA TACCCACCAC CACCTGCTGT AAGAGACTTA 
CTGCCGTCAG GCTCTAGAGA TGAGCCTCCA CCTGCCTCTC AGAGCACTAG CCAGGACTGT 
TCACAGGCTT TAAAACAGAG CCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310992.1
CDS	10..5556
Translation	MAAAPGLLVWLLVLRLPWRVPGQLDPSTGRRFSEHKLCADDECSMLMYRGEALEDFTGPDCRFVNFKKGDPVYVYYKLARGWPEVWAGSVGRTFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFHNYNVEELLGFLELYNSAATDSEKAVEKTLQDMEKNPELSKEREPEPEPVEANSEESDSVFSENTEDLQEQFTTQKHHSHANSQANHAQGEQASFESFEEMLQDKLKVPESENNKTSNSSQVSNEQDKIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDTEYYAVGKEDEENQEDFDELPLLTFTDGEDMKTPAKSGVEKYPTDKEQNSNEEDKVQLTVPPGIKNDDKNILTTWGDTIFSIVTGGEETRDTMDLESSSSEEEKEDDDDALVPDSKQGKPQSATDYSDPDNVDDGLFIVDIPKTNNDKEVNAEHHIKGKGRGVQESKRGLVQDKTELEDENQEGMTVHSSVHSNNLNSMPAAEKGKDTLKSAYDDTENDLKGAAIHISKGMLHEEKPGEQILEGGSESESAQKAAGNQMNDRKIQQESLGSAPLMGDDHPNASRDSVEGDALVNGAKLHTLSVEHQREELKEELVLKTQNQPRFSSPDEIDLPRELEDEVPILGRNLPWQQERDVAATASKQMSEKIRLSEGEAKEDSLDEEFFHHKAMQGTEVGQTDQTDSTGGPAFLSKVEEDDYPSEELLEDENAINAKRSKEKNPGNQGRQFDVNLQVPDRAVLGTIHPDPEIEESKQETSMILDSEKTSETAAKGVNTGGREPNTMVEKERPLADKKAQRPFERSDFSDSIKIQTPELGEVFQNKDSDYLKNDNPEEHLKTSGLAGEPEGELSKEDHENTEKYMGTESQGSAAAEPEDDSFHWTPHTSVEPGHSDKREDLLIISSFFKEQQSLQRFQKYFNVHELEALLQEMSSKLKSAQQESLPYNMEKVLDKVFRASESQILSIAEKMLDTRVAENRDLGMNENNIFEEAAVLDDIQDLIYFVRYKHSTAEETATLVMAPPLEEGLGGAMEEMQPLHEDNFSREKTAELNVQVPEEPTHLDQRVIGDTHASEVSQKPNTEKDLDPGPVTTEDTPMDAIDANKQPETAAEEPASVTPLENAILLIYSFMFYLTKSLVATLPDDVQPGPDFYGLPWKPVFITAFLGIASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREHRLSAADEKAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGKPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

Target ORF information:

RefSeq Version	NM_001324063.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324063.1

ORF Insert Sequence:

ATGGCTGCGG CGCCTGGGCT GCTCGTCTGG CTGCTCGTGC TCCGGCTGCC CTGGCGGGTG 
CCGGGCCAGC TGGACCCCAG CACTGGCCGG CGGTTCTCGG AGCACAAACT CTGCGCGGAC 
GACGAATGCA GCATGTTAAT GTACCGCGGT GAGGCTCTTG AAGATTTCAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGTGAT CCTGTATATG TTTACTATAA ACTGGCAAGA 
GGATGGCCTG AAGTTTGGGC TGGAAGTGTT GGACGCACTT TTGGATATTT TCCAAAAGAT 
TTAATCCAGG TAGTTCATGA ATATACCAAA GAAGAGCTAC AAGTTCCAAC AGATGAGACG 
GATTTTGTTT GTTTTGATGG AGGAAGAGAT GATTTTCATA ATTATAATGT AGAAGAACTT 
TTAGGGTTTT TGGAACTGTA CAATTCTGCA GCTACAGATT CTGAGAAAGC TGTAGAAAAA 
ACTTTACAGG ATATGGAAAA AAACCCTGAA TTATCTAAGG AAAGGGAACC TGAACCTGAA 
CCAGTAGAAG CCAACTCAGA GGAAAGTGAT AGTGTATTCT CAGAAAACAC TGAGGATCTT 
CAGGAACAGT TTACAACTCA GAAGCACCAC TCCCATGCAA ACAGCCAAGC AAATCATGCT 
CAGGGAGAGC AGGCTTCATT TGAATCTTTT GAAGAAATGC TGCAAGATAA ACTAAAAGTG 
CCAGAAAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCAAATGA ACAGGATAAG 
ATTGATGCCT ATAAACTTTT GAAAAAAGAA ATGACTCTAG ACTTGAAAAC CAAATTTGGC 
TCAACAGCTG ATGCACTTGT ATCTGATGAT GAGACAACCA GACTCGTTAC TTCATTAGAA 
GATGATTTTG ATGAGGAATT GGATACTGAG TATTATGCAG TTGGAAAGGA AGATGAGGAG 
AACCAAGAAG ACTTTGATGA GTTGCCATTA CTTACCTTTA CAGATGGGGA AGATATGAAA 
ACTCCAGCAA AGTCTGGCGT TGAGAAATAT CCAACAGATA AAGAGCAGAA TTCAAATGAA 
GAGGACAAGG TTCAGCTAAC TGTGCCCCCT GGCATCAAAA ATGATGATAA AAATATACTA 
ACAACCTGGG GGGACACTAT CTTCTCTATT GTCACAGGAG GTGAAGAAAC AAGAGATACG 
ATGGATTTAG AGAGCTCTAG TTCAGAGGAA GAAAAAGAAG ATGATGATGA TGCATTAGTC 
CCAGATAGCA AACAGGGGAA ACCACAGTCA GCAACAGATT ATAGTGACCC TGACAATGTA 
GATGATGGTC TTTTTATTGT AGACATTCCT AAAACAAATA ATGACAAAGA AGTAAACGCA 
GAACATCACA TTAAAGGAAA AGGGAGGGGA GTTCAGGAAT CCAAGAGGGG CCTGGTACAA 
GATAAGACAG AATTAGAGGA TGAAAATCAA GAAGGCATGA CTGTGCACAG TTCTGTTCAC 
AGCAATAACC TCAACTCTAT GCCAGCTGCT GAAAAGGGTA AAGACACATT AAAATCAGCT 
TATGATGATA CAGAAAATGA CCTAAAAGGA GCAGCTATTC ATATCTCAAA AGGAATGCTC 
CACGAAGAAA AGCCTGGAGA GCAGATTTTG GAAGGTGGCT CAGAGAGTGA ATCTGCACAG 
AAAGCTGCAG GGAATCAAAT GAATGACAGA AAGATTCAAC AGGAATCCCT GGGTAGTGCA 
CCACTCATGG GAGATGACCA CCCTAACGCA TCCAGAGACA GTGTGGAGGG AGACGCTTTG 
GTAAATGGGG CCAAACTGCA CACGCTTTCA GTGGAGCATC AACGTGAGGA ATTGAAAGAG 
GAATTAGTTC TTAAAACTCA AAACCAACCT AGATTCTCCT CTCCAGATGA GATTGATTTG 
CCCAGAGAAC TGGAAGACGA GGTTCCCATT CTGGGAAGAA ATCTTCCCTG GCAACAAGAA 
AGAGATGTGG CTGCCACAGC CAGTAAGCAA ATGAGTGAGA AGATAAGGCT CTCTGAGGGA 
GAAGCCAAAG AGGACTCCTT GGATGAAGAG TTTTTTCATC ACAAGGCAAT GCAGGGCACA 
GAGGTAGGAC AGACAGACCA AACTGACAGC ACAGGAGGAC CAGCTTTCCT TTCTAAAGTA 
GAAGAGGATG ATTATCCCTC TGAAGAACTA CTAGAGGATG AAAACGCTAT AAATGCAAAA 
CGGTCTAAAG AAAAAAACCC TGGGAATCAG GGCAGGCAGT TTGATGTTAA TCTGCAAGTC 
CCTGACAGAG CAGTTTTAGG GACCATTCAT CCAGATCCAG AAATTGAAGA AAGCAAGCAA 
GAAACTAGTA TGATTTTGGA TAGCGAAAAA ACAAGTGAGA CTGCTGCCAA AGGGGTCAAC 
ACAGGAGGCA GGGAACCAAA TACAATGGTG GAAAAAGAAC GCCCTCTGGC AGATAAGAAA 
GCACAGAGAC CATTTGAACG AAGTGACTTT TCTGACAGCA TAAAAATTCA GACTCCAGAA 
TTAGGTGAAG TGTTTCAGAA TAAAGATTCT GATTATCTGA AGAACGACAA CCCTGAGGAA 
CATCTGAAGA CCTCAGGGCT TGCAGGGGAG CCTGAGGGAG AACTCTCAAA AGAGGACCAT 
GAGAACACAG AGAAGTACAT GGGCACAGAA AGCCAGGGGT CTGCTGCTGC AGAACCTGAA 
GATGACTCGT TCCACTGGAC TCCACATACA AGTGTAGAGC CAGGGCATAG TGACAAGAGG 
GAGGACTTAC TTATCATAAG CAGCTTCTTT AAAGAACAAC AGTCTTTGCA GCGGTTCCAG 
AAGTACTTTA ATGTCCATGA GCTGGAAGCC TTGCTACAAG AAATGTCATC AAAACTGAAG 
TCAGCGCAGC AGGAGAGCCT GCCCTATAAT ATGGAAAAAG TCCTAGATAA GGTCTTCCGT 
GCTTCTGAGT CACAAATTCT GAGCATAGCA GAAAAAATGC TTGATACTCG TGTGGCTGAA 
AATAGAGATC TGGGAATGAA CGAAAATAAC ATATTTGAAG AGGCTGCAGT GCTTGATGAC 
ATTCAAGACC TCATCTATTT TGTCAGGTAC AAGCACTCCA CAGCAGAGGA GACAGCCACA 
CTGGTGATGG CACCACCTCT AGAGGAAGGC TTGGGTGGAG CAATGGAAGA GATGCAACCA 
CTGCATGAAG ATAATTTCTC ACGAGAGAAG ACAGCAGAAC TTAATGTGCA GGTTCCTGAA 
GAACCCACCC ACTTGGACCA ACGTGTGATT GGGGACACTC ATGCCTCAGA AGTGTCACAG 
AAGCCAAATA CTGAGAAAGA CCTGGACCCA GGGCCAGTTA CAACAGAAGA CACTCCTATG 
GATGCTATTG ATGCAAACAA GCAACCAGAG ACAGCCGCCG AAGAGCCGGC AAGTGTCACA 
CCTTTGGAAA ACGCAATCCT TCTAATATAT TCATTCATGT TTTATTTAAC TAAGTCGCTA 
GTTGCTACAT TGCCTGATGA TGTTCAGCCT GGGCCTGATT TTTATGGACT GCCATGGAAA 
CCTGTATTTA TCACTGCCTT CTTGGGAATT GCTTCGTTTG CCATTTTCTT ATGGAGAACT 
GTCCTTGTTG TGAAGGATAG AGTATATCAA GTCACGGAAC AGCAAATTTC TGAGAAGTTG 
AAGACTATCA TGAAAGAAAA TACAGAACTT GTACAAAAAT TGTCAAATTA TGAACAGAAG 
ATCAAGGAAT CAAAGAAACA TGTTCAGGAA ACCAGGAAAC AAAATATGAT TCTCTCTGAT 
GAAGCAATTA AATATAAGGA TAAAATCAAG ACACTTGAAA AAAATCAGGA AATTCTGGAT 
GACACAGCTA AAAATCTTCG TGTTATGCTA GAATCTGAGA GAGAACAGAA TGTCAAGAAT 
CAGGACTTGT TGCAGCAGGA AATCGAAGAC TGGAGTAAAT TACATGCTGA GCTCAGTGAG 
CAAATCAAAT CATTTGAGAA GTCTCAGAAA GATTTGGAAG TAGCTCTTAC TCACAAGGAT 
GATAATATTA ATGCTTTGAC TAACTGCATT ACACAGTTGA ATCTGTTAGA GTGTGAATCT 
GAATCTGAGG GTCAAAATAA AGGTGGAAAT GATTCAGATG AATTAGCAAA TGGAGAAGTG 
GGAGGTGACC GGAATGAGAA GATGAAAAAT CAAATTAAGC AGATGATGGA TGTCTCTCGG 
ACACAGACTG CAATATCGGT AGTTGAAGAG GATCTAAAGC TTTTACAGCT TAAGCTAAGA 
GCCTCCGTGT CCACTAAATG TAACCTGGAA GACCAGGTAA AGAAATTGGA AGATGACCGC 
AACTCACTAC AAGCTGCCAA AGCTGGACTG GAAGATGAAT GCAAAACCTT GAGGCAGAAA 
GTGGAGATTC TGAATGAGCT CTATCAGCAG AAGGAGATGG CTTTGCAAAA GAAACTGAGT 
CAAGAAGAGT ATGAACGGCA AGAAAGAGAG CACAGGCTGT CAGCTGCAGA TGAAAAGGCA 
GTTTCGGCTG CAGAGGAAGT AAAAACTTAC AAGCGGAGAA TTGAAGAAAT GGAGGATGAA 
TTACAGAAGA CAGAGCGGTC ATTTAAAAAC CAGATCGCTA CCCATGAGAA GAAAGCTCAT 
GAAAACTGGC TCAAAGCTCG TGCTGCAGAA AGAGCTATAG CTGAAGAGAA AAGGGAAGCT 
GCCAATTTGA GACACAAATT ATTAGAATTA ACACAAAAGA TGGCAATGCT GCAAGAAGAA 
CCTGTGATTG TAAAACCAAT GCCAGGAAAA CCAAATACAC AAAACCCTCC ACGGAGAGGT 
CCTCTGAGCC AGAATGGCTC TTTTGGCCCA TCCCCTGTGA GTGGTGGAGA ATGCTCCCCT 
CCATTGACAG TGGAGCCACC CGTGAGACCT CTCTCTGCTA CTCTCAATCG AAGAGATATG 
CCTAGAAGTG AATTTGGATC AGTGGACGGG CCTCTACCTC ATCCTCGATG GTCAGCTGAG 
GCATCTGGGA AACCCTCTCC TTCTGATCCA GGATCTGGTA CAGCTACCAT GATGAACAGC 
AGCTCAAGAG GCTCTTCCCC TACCAGGGTA CTCGATGAAG GCAAGGTTAA TATGGCTCCA 
AAAGGGCCCC CTCCTTTCCC AGGAGTCCCT CTCATGAGCA CCCCCATGGG AGGCCCTGTA 
CCACCACCCA TTCGATATGG ACCACCACCT CAGCTCTGCG GACCTTTTGG GCCTCGGCCA 
CTTCCTCCAC CCTTTGGCCC TGGTATGCGT CCACCACTAG GCTTAAGAGA ATTTGCACCA 
GGCGTTCCAC CAGGAAGACG GGACCTGCCT CTCCACCCTC GGGGATTTTT ACCTGGACAC 
GCACCATTTA GACCTTTAGG TTCACTTGGC CCAAGAGAGT ACTTTATTCC TGGTACCCGA 
TTACCACCCC CAACCCATGG TCCCCAGGAA TACCCACCAC CACCTGCTGT AAGAGACTTA 
CTGCCGTCAG GCTCTAGAGA TGAGCCTCCA CCTGCCTCTC AGAGCACTAG CCAGGACTGT 
TCACAGGCTT TAAAACAGAG CCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_006711304.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_006711367.3 Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant X1, mRNA.

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CloneID	OHu34359
Clone ID Related Accession (Same CDS sequence)	XM_006711304.4
Accession Version	XM_006711304.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transport and Golgi organization protein 1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006711304.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG CGCCTGGGCT GCTCGTCTGG CTGCTCGTGC TCCGGCTGCC CTGGCGGGTG 
CCGGGCCAGC TGGACCCCAG CACTGGCCGG CGGTTCTCGG AGCACAAACT CTGCGCGGAC 
GACGAATGCA GCATGTTAAT GTACCGCGGT GAGGCTCTTG AAGATTTCAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGTGAT CCTGTATATG TTTACTATAA ACTGGCAAGA 
GGATGGCCTG AAGTTTGGGC TGGAAGTGTT GGACGCACTT TTGGATATTT TCCAAAAGAT 
TTAATCCAGG TAGTTCATGA ATATACCAAA GAAGAGCTAC AAGTTCCAAC AGATGAGACG 
GATTTTGTTT GTTTTGATGG AGGAAGAGAT GATTTTCATA ATTATAATGT AGAAGAACTT 
TTAGGGTTTT TGGAACTGTA CAATTCTGCA GCTACAGATT CTGAGAAAGC TGTAGAAAAA 
ACTTTACAGG ATATGGAAAA AAACCCTGAA TTATCTAAGG AAAGGGAACC TGAACCTGAA 
CCAGTAGAAG CCAACTCAGA GGAAAGTGAT AGTGTATTCT CAGAAAACAC TGAGGATCTT 
CAGGAACAGT TTACAACTCA GAAGCACCAC TCCCATGCAA ACAGCCAAGC AAATCATGCT 
CAGGGAGAGC AGGCTTCATT TGAATCTTTT GAAGAAATGC TGCAAGATAA ACTAAAAGTG 
CCAGAAAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCAAATGA ACAGGATAAG 
ATTGATGCCT ATAAACTTTT GAAAAAAGAA ATGACTCTAG ACTTGAAAAC CAAATTTGGC 
TCAACAGCTG ATGCACTTGT ATCTGATGAT GAGACAACCA GACTCGTTAC TTCATTAGAA 
GATGATTTTG ATGAGGAATT GGATACTGAG TATTATGCAG TTGGAAAGGA AGATGAGGAG 
AACCAAGAAG ACTTTGATGA GTTGCCATTA CTTACCTTTA CAGATGGGGA AGATATGAAA 
ACTCCAGCAA AGTCTGGCGT TGAGAAATAT CCAACAGATA AAGAGCAGAA TTCAAATGAA 
GAGGACAAGG TTCAGCTAAC TGTGCCCCCT GGCATCAAAA ATGATGATAA AAATATACTA 
ACAACCTGGG GGGACACTAT CTTCTCTATT GTCACAGGAG GTGAAGAAAC AAGAGATACG 
ATGGATTTAG AGAGCTCTAG TTCAGAGGAA GAAAAAGAAG ATGATGATGA TGCATTAGTC 
CCAGATAGCA AACAGGGGAA ACCACAGTCA GCAACAGATT ATAGTGACCC TGACAATGTA 
GATGATGGTC TTTTTATTGT AGACATTCCT AAAACAAATA ATGACAAAGA AGTAAACGCA 
GAACATCACA TTAAAGGAAA AGGGAGGGGA GTTCAGGAAT CCAAGAGGGG CCTGGTACAA 
GATAAGACAG AATTAGAGGA TGAAAATCAA GAAGGCATGA CTGTGCACAG TTCTGTTCAC 
AGCAATAACC TCAACTCTAT GCCAGCTGCT GAAAAGGGTA AAGACACATT AAAATCAGCT 
TATGATGATA CAGAAAATGA CCTAAAAGGA GCAGCTATTC ATATCTCAAA AGGAATGCTC 
CACGAAGAAA AGCCTGGAGA GCAGATTTTG GAAGGTGGCT CAGAGAGTGA ATCTGCACAG 
AAAGCTGCAG GGAATCAAAT GAATGACAGA AAGATTCAAC AGGAATCCCT GGGTAGTGCA 
CCACTCATGG GAGATGACCA CCCTAACGCA TCCAGAGACA GTGTGGAGGG AGACGCTTTG 
GTAAATGGGG CCAAACTGCA CACGCTTTCA GTGGAGCATC AACGTGAGGA ATTGAAAGAG 
GAATTAGTTC TTAAAACTCA AAACCAACCT AGATTCTCCT CTCCAGATGA GATTGATTTG 
CCCAGAGAAC TGGAAGACGA GGTTCCCATT CTGGGAAGAA ATCTTCCCTG GCAACAAGAA 
AGAGATGTGG CTGCCACAGC CAGTAAGCAA ATGAGTGAGA AGATAAGGCT CTCTGAGGGA 
GAAGCCAAAG AGGACTCCTT GGATGAAGAG TTTTTTCATC ACAAGGCAAT GCAGGGCACA 
GAGGTAGGAC AGACAGACCA AACTGACAGC ACAGGAGGAC CAGCTTTCCT TTCTAAAGTA 
GAAGAGGATG ATTATCCCTC TGAAGAACTA CTAGAGGATG AAAACGCTAT AAATGCAAAA 
CGGTCTAAAG AAAAAAACCC TGGGAATCAG GGCAGGCAGT TTGATGTTAA TCTGCAAGTC 
CCTGACAGAG CAGTTTTAGG GACCATTCAT CCAGATCCAG AAATTGAAGA AAGCAAGCAA 
GAAACTAGTA TGATTTTGGA TAGCGAAAAA ACAAGTGAGA CTGCTGCCAA AGGGGTCAAC 
ACAGGAGGCA GGGAACCAAA TACAATGGTG GAAAAAGAAC GCCCTCTGGC AGATAAGAAA 
GCACAGAGAC CATTTGAACG AAGTGACTTT TCTGACAGCA TAAAAATTCA GACTCCAGAA 
TTAGGTGAAG TGTTTCAGAA TAAAGATTCT GATTATCTGA AGAACGACAA CCCTGAGGAA 
CATCTGAAGA CCTCAGGGCT TGCAGGGGAG CCTGAGGGAG AACTCTCAAA AGAGGACCAT 
GAGAACACAG AGAAGTACAT GGGCACAGAA AGCCAGGGGT CTGCTGCTGC AGAACCTGAA 
GATGACTCGT TCCACTGGAC TCCACATACA AGTGTAGAGC CAGGGCATAG TGACAAGAGG 
GAGGACTTAC TTATCATAAG CAGCTTCTTT AAAGAACAAC AGTCTTTGCA GCGGTTCCAG 
AAGTACTTTA ATGTCCATGA GCTGGAAGCC TTGCTACAAG AAATGTCATC AAAACTGAAG 
TCAGCGCAGC AGGAGAGCCT GCCCTATAAT ATGGAAAAAG TCCTAGATAA GGTCTTCCGT 
GCTTCTGAGT CACAAATTCT GAGCATAGCA GAAAAAATGC TTGATACTCG TGTGGCTGAA 
AATAGAGATC TGGGAATGAA CGAAAATAAC ATATTTGAAG AGGCTGCAGT GCTTGATGAC 
ATTCAAGACC TCATCTATTT TGTCAGGTAC AAGCACTCCA CAGCAGAGGA GACAGCCACA 
CTGGTGATGG CACCACCTCT AGAGGAAGGC TTGGGTGGAG CAATGGAAGA GATGCAACCA 
CTGCATGAAG ATAATTTCTC ACGAGAGAAG ACAGCAGAAC TTAATGTGCA GGTTCCTGAA 
GAACCCACCC ACTTGGACCA ACGTGTGATT GGGGACACTC ATGCCTCAGA AGTGTCACAG 
AAGCCAAATA CTGAGAAAGA CCTGGACCCA GGGCCAGTTA CAACAGAAGA CACTCCTATG 
GATGCTATTG ATGCAAACAA GCAACCAGAG ACAGCCGCCG AAGAGCCGGC AAGTGTCACA 
CCTTTGGAAA ACGCAATCCT TCTAATATAT TCATTCATGT TTTATTTAAC TAAGTCGCTA 
GTTGCTACAT TGCCTGATGA TGTTCAGCCT GGGCCTGATT TTTATGGACT GCCATGGAAA 
CCTGTATTTA TCACTGCCTT CTTGGGAATT GCTTCGTTTG CCATTTTCTT ATGGAGAACT 
GTCCTTGTTG TGAAGGATAG AGTATATCAA GTCACGGAAC AGCAAATTTC TGAGAAGTTG 
AAGACTATCA TGAAAGAAAA TACAGAACTT GTACAAAAAT TGTCAAATTA TGAACAGAAG 
ATCAAGGAAT CAAAGAAACA TGTTCAGGAA ACCAGGAAAC AAAATATGAT TCTCTCTGAT 
GAAGCAATTA AATATAAGGA TAAAATCAAG ACACTTGAAA AAAATCAGGA AATTCTGGAT 
GACACAGCTA AAAATCTTCG TGTTATGCTA GAATCTGAGA GAGAACAGAA TGTCAAGAAT 
CAGGACTTGT TGCAGCAGGA AATCGAAGAC TGGAGTAAAT TACATGCTGA GCTCAGTGAG 
CAAATCAAAT CATTTGAGAA GTCTCAGAAA GATTTGGAAG TAGCTCTTAC TCACAAGGAT 
GATAATATTA ATGCTTTGAC TAACTGCATT ACACAGTTGA ATCTGTTAGA GTGTGAATCT 
GAATCTGAGG GTCAAAATAA AGGTGGAAAT GATTCAGATG AATTAGCAAA TGGAGAAGTG 
GGAGGTGACC GGAATGAGAA GATGAAAAAT CAAATTAAGC AGATGATGGA TGTCTCTCGG 
ACACAGACTG CAATATCGGT AGTTGAAGAG GATCTAAAGC TTTTACAGCT TAAGCTAAGA 
GCCTCCGTGT CCACTAAATG TAACCTGGAA GACCAGGTAA AGAAATTGGA AGATGACCGC 
AACTCACTAC AAGCTGCCAA AGCTGGACTG GAAGATGAAT GCAAAACCTT GAGGCAGAAA 
GTGGAGATTC TGAATGAGCT CTATCAGCAG AAGGAGATGG CTTTGCAAAA GAAACTGAGT 
CAAGAAGAGT ATGAACGGCA AGAAAGAGAG CACAGGCTGT CAGCTGCAGA TGAAAAGGCA 
GTTTCGGCTG CAGAGGAAGT AAAAACTTAC AAGCGGAGAA TTGAAGAAAT GGAGGATGAA 
TTACAGAAGA CAGAGCGGTC ATTTAAAAAC CAGATCGCTA CCCATGAGAA GAAAGCTCAT 
GAAAACTGGC TCAAAGCTCG TGCTGCAGAA AGAGCTATAG CTGAAGAGAA AAGGGAAGCT 
GCCAATTTGA GACACAAATT ATTAGAATTA ACACAAAAGA TGGCAATGCT GCAAGAAGAA 
CCTGTGATTG TAAAACCAAT GCCAGGAAAA CCAAATACAC AAAACCCTCC ACGGAGAGGT 
CCTCTGAGCC AGAATGGCTC TTTTGGCCCA TCCCCTGTGA GTGGTGGAGA ATGCTCCCCT 
CCATTGACAG TGGAGCCACC CGTGAGACCT CTCTCTGCTA CTCTCAATCG AAGAGATATG 
CCTAGAAGTG AATTTGGATC AGTGGACGGG CCTCTACCTC ATCCTCGATG GTCAGCTGAG 
GCATCTGGGA AACCCTCTCC TTCTGATCCA GGATCTGGTA CAGCTACCAT GATGAACAGC 
AGCTCAAGAG GCTCTTCCCC TACCAGGGTA CTCGATGAAG GCAAGCAAAC TGTTCTCCAA 
GAGCCTGAAG TCCCCTCAGT TCCCAGCATT ACATCTTTGG CTGAGCGTCC AGTAGCAGTT 
AATATGGCTC CAAAAGGGCC CCCTCCTTTC CCAGGAGTCC CTCTCATGAG CACCCCCATG 
GGAGGCCCTG TACCACCACC CATTCGATAT GGACCACCAC CTCAGCTCTG CGGACCTTTT 
GGGCCTCGGC CACTTCCTCC ACCCTTTGGC CCTGGTATGC GTCCACCACT AGGCTTAAGA 
GAATTTGCAC CAGGCGTTCC ACCAGGAAGA CGGGACCTGC CTCTCCACCC TCGGGGATTT 
TTACCTGGAC ACGCACCATT TAGACCTTTA GGTTCACTTG GCCCAAGAGA GTACTTTATT 
CCTGGTACCC GATTACCACC CCCAACCCAT GGTCCCCAGG AATACCCACC ACCACCTGCT 
GTAAGAGACT TACTGCCGTC AGGCTCTAGA GATGAGCCTC CACCTGCCTC TCAGAGCACT 
AGCCAGGACT GTTCACAGGC TTTAAAACAG AGCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006711367.3
CDS	10..5628
Translation	MAAAPGLLVWLLVLRLPWRVPGQLDPSTGRRFSEHKLCADDECSMLMYRGEALEDFTGPDCRFVNFKKGDPVYVYYKLARGWPEVWAGSVGRTFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFHNYNVEELLGFLELYNSAATDSEKAVEKTLQDMEKNPELSKEREPEPEPVEANSEESDSVFSENTEDLQEQFTTQKHHSHANSQANHAQGEQASFESFEEMLQDKLKVPESENNKTSNSSQVSNEQDKIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDTEYYAVGKEDEENQEDFDELPLLTFTDGEDMKTPAKSGVEKYPTDKEQNSNEEDKVQLTVPPGIKNDDKNILTTWGDTIFSIVTGGEETRDTMDLESSSSEEEKEDDDDALVPDSKQGKPQSATDYSDPDNVDDGLFIVDIPKTNNDKEVNAEHHIKGKGRGVQESKRGLVQDKTELEDENQEGMTVHSSVHSNNLNSMPAAEKGKDTLKSAYDDTENDLKGAAIHISKGMLHEEKPGEQILEGGSESESAQKAAGNQMNDRKIQQESLGSAPLMGDDHPNASRDSVEGDALVNGAKLHTLSVEHQREELKEELVLKTQNQPRFSSPDEIDLPRELEDEVPILGRNLPWQQERDVAATASKQMSEKIRLSEGEAKEDSLDEEFFHHKAMQGTEVGQTDQTDSTGGPAFLSKVEEDDYPSEELLEDENAINAKRSKEKNPGNQGRQFDVNLQVPDRAVLGTIHPDPEIEESKQETSMILDSEKTSETAAKGVNTGGREPNTMVEKERPLADKKAQRPFERSDFSDSIKIQTPELGEVFQNKDSDYLKNDNPEEHLKTSGLAGEPEGELSKEDHENTEKYMGTESQGSAAAEPEDDSFHWTPHTSVEPGHSDKREDLLIISSFFKEQQSLQRFQKYFNVHELEALLQEMSSKLKSAQQESLPYNMEKVLDKVFRASESQILSIAEKMLDTRVAENRDLGMNENNIFEEAAVLDDIQDLIYFVRYKHSTAEETATLVMAPPLEEGLGGAMEEMQPLHEDNFSREKTAELNVQVPEEPTHLDQRVIGDTHASEVSQKPNTEKDLDPGPVTTEDTPMDAIDANKQPETAAEEPASVTPLENAILLIYSFMFYLTKSLVATLPDDVQPGPDFYGLPWKPVFITAFLGIASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREHRLSAADEKAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGKPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKQTVLQEPEVPSVPSITSLAERPVAVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_006711304.4
Organism	Homo sapiens(human)
Definition	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006711304.4

ORF Insert Sequence:

ATGGCTGCGG CGCCTGGGCT GCTCGTCTGG CTGCTCGTGC TCCGGCTGCC CTGGCGGGTG 
CCGGGCCAGC TGGACCCCAG CACTGGCCGG CGGTTCTCGG AGCACAAACT CTGCGCGGAC 
GACGAATGCA GCATGTTAAT GTACCGCGGT GAGGCTCTTG AAGATTTCAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGTGAT CCTGTATATG TTTACTATAA ACTGGCAAGA 
GGATGGCCTG AAGTTTGGGC TGGAAGTGTT GGACGCACTT TTGGATATTT TCCAAAAGAT 
TTAATCCAGG TAGTTCATGA ATATACCAAA GAAGAGCTAC AAGTTCCAAC AGATGAGACG 
GATTTTGTTT GTTTTGATGG AGGAAGAGAT GATTTTCATA ATTATAATGT AGAAGAACTT 
TTAGGGTTTT TGGAACTGTA CAATTCTGCA GCTACAGATT CTGAGAAAGC TGTAGAAAAA 
ACTTTACAGG ATATGGAAAA AAACCCTGAA TTATCTAAGG AAAGGGAACC TGAACCTGAA 
CCAGTAGAAG CCAACTCAGA GGAAAGTGAT AGTGTATTCT CAGAAAACAC TGAGGATCTT 
CAGGAACAGT TTACAACTCA GAAGCACCAC TCCCATGCAA ACAGCCAAGC AAATCATGCT 
CAGGGAGAGC AGGCTTCATT TGAATCTTTT GAAGAAATGC TGCAAGATAA ACTAAAAGTG 
CCAGAAAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCAAATGA ACAGGATAAG 
ATTGATGCCT ATAAACTTTT GAAAAAAGAA ATGACTCTAG ACTTGAAAAC CAAATTTGGC 
TCAACAGCTG ATGCACTTGT ATCTGATGAT GAGACAACCA GACTCGTTAC TTCATTAGAA 
GATGATTTTG ATGAGGAATT GGATACTGAG TATTATGCAG TTGGAAAGGA AGATGAGGAG 
AACCAAGAAG ACTTTGATGA GTTGCCATTA CTTACCTTTA CAGATGGGGA AGATATGAAA 
ACTCCAGCAA AGTCTGGCGT TGAGAAATAT CCAACAGATA AAGAGCAGAA TTCAAATGAA 
GAGGACAAGG TTCAGCTAAC TGTGCCCCCT GGCATCAAAA ATGATGATAA AAATATACTA 
ACAACCTGGG GGGACACTAT CTTCTCTATT GTCACAGGAG GTGAAGAAAC AAGAGATACG 
ATGGATTTAG AGAGCTCTAG TTCAGAGGAA GAAAAAGAAG ATGATGATGA TGCATTAGTC 
CCAGATAGCA AACAGGGGAA ACCACAGTCA GCAACAGATT ATAGTGACCC TGACAATGTA 
GATGATGGTC TTTTTATTGT AGACATTCCT AAAACAAATA ATGACAAAGA AGTAAACGCA 
GAACATCACA TTAAAGGAAA AGGGAGGGGA GTTCAGGAAT CCAAGAGGGG CCTGGTACAA 
GATAAGACAG AATTAGAGGA TGAAAATCAA GAAGGCATGA CTGTGCACAG TTCTGTTCAC 
AGCAATAACC TCAACTCTAT GCCAGCTGCT GAAAAGGGTA AAGACACATT AAAATCAGCT 
TATGATGATA CAGAAAATGA CCTAAAAGGA GCAGCTATTC ATATCTCAAA AGGAATGCTC 
CACGAAGAAA AGCCTGGAGA GCAGATTTTG GAAGGTGGCT CAGAGAGTGA ATCTGCACAG 
AAAGCTGCAG GGAATCAAAT GAATGACAGA AAGATTCAAC AGGAATCCCT GGGTAGTGCA 
CCACTCATGG GAGATGACCA CCCTAACGCA TCCAGAGACA GTGTGGAGGG AGACGCTTTG 
GTAAATGGGG CCAAACTGCA CACGCTTTCA GTGGAGCATC AACGTGAGGA ATTGAAAGAG 
GAATTAGTTC TTAAAACTCA AAACCAACCT AGATTCTCCT CTCCAGATGA GATTGATTTG 
CCCAGAGAAC TGGAAGACGA GGTTCCCATT CTGGGAAGAA ATCTTCCCTG GCAACAAGAA 
AGAGATGTGG CTGCCACAGC CAGTAAGCAA ATGAGTGAGA AGATAAGGCT CTCTGAGGGA 
GAAGCCAAAG AGGACTCCTT GGATGAAGAG TTTTTTCATC ACAAGGCAAT GCAGGGCACA 
GAGGTAGGAC AGACAGACCA AACTGACAGC ACAGGAGGAC CAGCTTTCCT TTCTAAAGTA 
GAAGAGGATG ATTATCCCTC TGAAGAACTA CTAGAGGATG AAAACGCTAT AAATGCAAAA 
CGGTCTAAAG AAAAAAACCC TGGGAATCAG GGCAGGCAGT TTGATGTTAA TCTGCAAGTC 
CCTGACAGAG CAGTTTTAGG GACCATTCAT CCAGATCCAG AAATTGAAGA AAGCAAGCAA 
GAAACTAGTA TGATTTTGGA TAGCGAAAAA ACAAGTGAGA CTGCTGCCAA AGGGGTCAAC 
ACAGGAGGCA GGGAACCAAA TACAATGGTG GAAAAAGAAC GCCCTCTGGC AGATAAGAAA 
GCACAGAGAC CATTTGAACG AAGTGACTTT TCTGACAGCA TAAAAATTCA GACTCCAGAA 
TTAGGTGAAG TGTTTCAGAA TAAAGATTCT GATTATCTGA AGAACGACAA CCCTGAGGAA 
CATCTGAAGA CCTCAGGGCT TGCAGGGGAG CCTGAGGGAG AACTCTCAAA AGAGGACCAT 
GAGAACACAG AGAAGTACAT GGGCACAGAA AGCCAGGGGT CTGCTGCTGC AGAACCTGAA 
GATGACTCGT TCCACTGGAC TCCACATACA AGTGTAGAGC CAGGGCATAG TGACAAGAGG 
GAGGACTTAC TTATCATAAG CAGCTTCTTT AAAGAACAAC AGTCTTTGCA GCGGTTCCAG 
AAGTACTTTA ATGTCCATGA GCTGGAAGCC TTGCTACAAG AAATGTCATC AAAACTGAAG 
TCAGCGCAGC AGGAGAGCCT GCCCTATAAT ATGGAAAAAG TCCTAGATAA GGTCTTCCGT 
GCTTCTGAGT CACAAATTCT GAGCATAGCA GAAAAAATGC TTGATACTCG TGTGGCTGAA 
AATAGAGATC TGGGAATGAA CGAAAATAAC ATATTTGAAG AGGCTGCAGT GCTTGATGAC 
ATTCAAGACC TCATCTATTT TGTCAGGTAC AAGCACTCCA CAGCAGAGGA GACAGCCACA 
CTGGTGATGG CACCACCTCT AGAGGAAGGC TTGGGTGGAG CAATGGAAGA GATGCAACCA 
CTGCATGAAG ATAATTTCTC ACGAGAGAAG ACAGCAGAAC TTAATGTGCA GGTTCCTGAA 
GAACCCACCC ACTTGGACCA ACGTGTGATT GGGGACACTC ATGCCTCAGA AGTGTCACAG 
AAGCCAAATA CTGAGAAAGA CCTGGACCCA GGGCCAGTTA CAACAGAAGA CACTCCTATG 
GATGCTATTG ATGCAAACAA GCAACCAGAG ACAGCCGCCG AAGAGCCGGC AAGTGTCACA 
CCTTTGGAAA ACGCAATCCT TCTAATATAT TCATTCATGT TTTATTTAAC TAAGTCGCTA 
GTTGCTACAT TGCCTGATGA TGTTCAGCCT GGGCCTGATT TTTATGGACT GCCATGGAAA 
CCTGTATTTA TCACTGCCTT CTTGGGAATT GCTTCGTTTG CCATTTTCTT ATGGAGAACT 
GTCCTTGTTG TGAAGGATAG AGTATATCAA GTCACGGAAC AGCAAATTTC TGAGAAGTTG 
AAGACTATCA TGAAAGAAAA TACAGAACTT GTACAAAAAT TGTCAAATTA TGAACAGAAG 
ATCAAGGAAT CAAAGAAACA TGTTCAGGAA ACCAGGAAAC AAAATATGAT TCTCTCTGAT 
GAAGCAATTA AATATAAGGA TAAAATCAAG ACACTTGAAA AAAATCAGGA AATTCTGGAT 
GACACAGCTA AAAATCTTCG TGTTATGCTA GAATCTGAGA GAGAACAGAA TGTCAAGAAT 
CAGGACTTGT TGCAGCAGGA AATCGAAGAC TGGAGTAAAT TACATGCTGA GCTCAGTGAG 
CAAATCAAAT CATTTGAGAA GTCTCAGAAA GATTTGGAAG TAGCTCTTAC TCACAAGGAT 
GATAATATTA ATGCTTTGAC TAACTGCATT ACACAGTTGA ATCTGTTAGA GTGTGAATCT 
GAATCTGAGG GTCAAAATAA AGGTGGAAAT GATTCAGATG AATTAGCAAA TGGAGAAGTG 
GGAGGTGACC GGAATGAGAA GATGAAAAAT CAAATTAAGC AGATGATGGA TGTCTCTCGG 
ACACAGACTG CAATATCGGT AGTTGAAGAG GATCTAAAGC TTTTACAGCT TAAGCTAAGA 
GCCTCCGTGT CCACTAAATG TAACCTGGAA GACCAGGTAA AGAAATTGGA AGATGACCGC 
AACTCACTAC AAGCTGCCAA AGCTGGACTG GAAGATGAAT GCAAAACCTT GAGGCAGAAA 
GTGGAGATTC TGAATGAGCT CTATCAGCAG AAGGAGATGG CTTTGCAAAA GAAACTGAGT 
CAAGAAGAGT ATGAACGGCA AGAAAGAGAG CACAGGCTGT CAGCTGCAGA TGAAAAGGCA 
GTTTCGGCTG CAGAGGAAGT AAAAACTTAC AAGCGGAGAA TTGAAGAAAT GGAGGATGAA 
TTACAGAAGA CAGAGCGGTC ATTTAAAAAC CAGATCGCTA CCCATGAGAA GAAAGCTCAT 
GAAAACTGGC TCAAAGCTCG TGCTGCAGAA AGAGCTATAG CTGAAGAGAA AAGGGAAGCT 
GCCAATTTGA GACACAAATT ATTAGAATTA ACACAAAAGA TGGCAATGCT GCAAGAAGAA 
CCTGTGATTG TAAAACCAAT GCCAGGAAAA CCAAATACAC AAAACCCTCC ACGGAGAGGT 
CCTCTGAGCC AGAATGGCTC TTTTGGCCCA TCCCCTGTGA GTGGTGGAGA ATGCTCCCCT 
CCATTGACAG TGGAGCCACC CGTGAGACCT CTCTCTGCTA CTCTCAATCG AAGAGATATG 
CCTAGAAGTG AATTTGGATC AGTGGACGGG CCTCTACCTC ATCCTCGATG GTCAGCTGAG 
GCATCTGGGA AACCCTCTCC TTCTGATCCA GGATCTGGTA CAGCTACCAT GATGAACAGC 
AGCTCAAGAG GCTCTTCCCC TACCAGGGTA CTCGATGAAG GCAAGCAAAC TGTTCTCCAA 
GAGCCTGAAG TCCCCTCAGT TCCCAGCATT ACATCTTTGG CTGAGCGTCC AGTAGCAGTT 
AATATGGCTC CAAAAGGGCC CCCTCCTTTC CCAGGAGTCC CTCTCATGAG CACCCCCATG 
GGAGGCCCTG TACCACCACC CATTCGATAT GGACCACCAC CTCAGCTCTG CGGACCTTTT 
GGGCCTCGGC CACTTCCTCC ACCCTTTGGC CCTGGTATGC GTCCACCACT AGGCTTAAGA 
GAATTTGCAC CAGGCGTTCC ACCAGGAAGA CGGGACCTGC CTCTCCACCC TCGGGGATTT 
TTACCTGGAC ACGCACCATT TAGACCTTTA GGTTCACTTG GCCCAAGAGA GTACTTTATT 
CCTGGTACCC GATTACCACC CCCAACCCAT GGTCCCCAGG AATACCCACC ACCACCTGCT 
GTAAGAGACT TACTGCCGTC AGGCTCTAGA GATGAGCCTC CACCTGCCTC TCAGAGCACT 
AGCCAGGACT GTTCACAGGC TTTAAAACAG AGCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_011509513.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507815.3 Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant X3, mRNA.

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CloneID	OHu79727
Clone ID Related Accession (Same CDS sequence)	XM_011509513.3
Accession Version	XM_011509513.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4662bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transport and Golgi organization protein 1 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509513.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG CGCCTGGGCT GCTCGTCTGG CTGCTCGTGC TCCGGCTGCC CTGGCGGGTG 
CCGGGCCAGC TGGACCCCAG CACTGGCCGG CGGTTCTCGG AGCACAAACT CTGCGCGGAC 
GACGAATGCA GCATGTTAAT GTACCGCGGT GAGGCTCTTG AAGATTTCAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGTGAT CCTGTATATG TTTACTATAA ACTGGCAAGA 
GGATGGCCTG AAGTTTGGGC TGGAAGTGTT GGACGCACTT TTGGATATTT TCCAAAAGAT 
TTAATCCAGG TAGTTCATGA ATATACCAAA GAAGAGCTAC AAGTTCCAAC AGATGAGACG 
GATTTTGTTT GTTTTGATGG AGGAAGAGAT GATTTTCATA ATTATAATGT AGAAGAACTT 
TTAGGGTTTT TGGAACTGTA CAATTCTGCA GCTACAGATT CTGAGAAAGC TGTAGAAAAA 
ACTTTACAGG ATATGGAAAA AAACCCTGAA TTATCTAAGG AAAGGGAACC TGAACCTGAA 
CCAGTAGAAG CCAACTCAGA GGAAAGTGAT AGTGTATTCT CAGAAAACAC TGAGGATCTT 
CAGGAACAGT TTACAACTCA GAAGCACCAC TCCCATGCAA ACAGCCAAGC AAATCATGCT 
CAGGGAGAGC AGGCTTCATT TGAATCTTTT GAAGAAATGC TGCAAGATAA ACTAAAAGTG 
CCAGAAAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCAAATGA ACAGGATAAG 
ATTGATGCCT ATAAACTTTT GAAAAAAGAA ATGACTCTAG ACTTGAAAAC CAAATTTGGC 
TCAACAGCTG ATGCACTTGT ATCTGATGAT GAGACAACCA GACTCGTTAC TTCATTAGAA 
GATGATTTTG ATGAGGAATT GGATACTGAG TATTATGCAG TTGGAAAGGA AGATGAGGAG 
AACCAAGAAG ACTTTGATGA GTTGCCATTA CTTACCTTTA CAGATGGGGA AGATATGAAA 
ACTCCAGCAA AGTCTGGCGT TGAGAAATAT CCAACAGATA AAGAGCAGAA TTCAAATGAA 
GAGGACAAGG TTCAGCTAAC TGTGCCCCCT GGCATCAAAA ATGATGATAA AAATATACTA 
ACAACCTGGG GGGACACTAT CTTCTCTATT GTCACAGGAG GTGAAGAAAC AAGAGATACG 
ATGGATTTAG AGAGCTCTAG TTCAGAGGAA GAAAAAGAAG ATGATGATGA TGCATTAGTC 
CCAGATAGCA AACAGGGGAA ACCACAGTCA GCAACAGATT ATAGTGACCC TGACAATGTA 
GATGATGGTC TTTTTATTGT AGACATTCCT AAAACAAATA ATGACAAAGA AGTAAACGCA 
GAACATCACA TTAAAGGAAA AGGGAGGGGA GTTCAGGAAT CCAAGAGGGG CCTGGTACAA 
GATAAGACAG AATTAGAGGA TGAAAATCAA GAAGGCATGA CTGTGCACAG TTCTGTTCAC 
AGCAATAACC TCAACTCTAT GCCAGCTGCT GAAAAGGGTA AAGACACATT AAAATCAGCT 
TATGATGATA CAGAAAATGA CCTAAAAGGA GCAGCTATTC ATATCTCAAA AGGAATGCTC 
CACGAAGAAA AGCCTGGAGA GCAGATTTTG GAAGGTGGCT CAGAGAGTGA ATCTGCACAG 
AAAGCTGCAG GGAATCAAAT GAATGACAGA AAGATTCAAC AGGAATCCCT GGGTAGTGCA 
CCACTCATGG GAGATGACCA CCCTAACGCA TCCAGAGACA GTGTGGAGGG AGACGCTTTG 
GTAAATGGGG CCAAACTGCA CACGCTTTCA GTGGAGCATC AACGTGAGGA ATTGAAAGAG 
GAATTAGTTC TTAAAACTCA AAACCAACCT AGATTCTCCT CTCCAGATGA GATTGATTTG 
CCCAGAGAAC TGGAAGACGA GGTTCCCATT CTGGGAAGAA ATCTTCCCTG GCAACAAGAA 
AGAGATGTGG CTGCCACAGC CAGTAAGCAA ATGAGTGAGA AGATAAGGCT CTCTGAGGGA 
GAAGCCAAAG AGGACTCCTT GGATGAAGAG TTTTTTCATC ACAAGGCAAT GCAGGGCACA 
GAGGTAGGAC AGACAGACCA AACTGACAGC ACAGGAGGAC CAGCTTTCCT TTCTAAAGTA 
GAAGAGGATG ATTATCCCTC TGAAGAACTA CTAGAGGATG AAAACGCTAT AAATGCAAAA 
CGGTCTAAAG AAAAAAACCC TGGGAATCAG GGCAGGCAGT TTGATGTTAA TCTGCAAGTC 
CCTGACAGAG CAGTTTTAGG GACCATTCAT CCAGATCCAG AAATTGAAGA AAGCAAGCAA 
GAAACTAGTA TGATTTTGGA TAGCGAAAAA ACAAGTGAGA CTGCTGCCAA AGGGGTCAAC 
ACAGGAGGCA GGGAACCAAA TACAATGGTG GAAAAAGAAC GCCCTCTGGC AGATAAGAAA 
GCACAGAGAC CATTTGAACG AAGTGACTTT TCTGACAGCA TAAAAATTCA GACTCCAGAA 
TTAGGTGAAG TGTTTCAGAA TAAAGATTCT GATTATCTGA AGAACGACAA CCCTGAGGAA 
CATCTGAAGA CCTCAGGGCT TGCAGGGGAG CCTGAGGGAG AACTCTCAAA AGAGGACCAT 
GAGAACACAG AGAAGTACAT GGGCACAGAA AGCCAGGGGT CTGCTGCTGC AGAACCTGAA 
GATGACTCGT TCCACTGGAC TCCACATACA AGTGTAGAGC CAGGGCATAG TGACAAGAGG 
GAGGACTTAC TTATCATAAG CAGCTTCTTT AAAGAACAAC AGTCTTTGCA GCGGTTCCAG 
AAGTACTTTA ATGTCCATGA GCTGGAAGCC TTGCTACAAG AAATGTCATC AAAACTGAAG 
TCAGCGCAGC AGGAGAGCCT GCCCTATAAT ATGGAAAAAG TCCTAGATAA GGTCTTCCGT 
GCTTCTGAGT CACAAATTCT GAGCATAGCA GAAAAAATGC TTGATACTCG TGTGGCTGAA 
AATAGAGATC TGGGAATGAA CGAAAATAAC ATATTTGAAG AGGCTGCAGT GCTTGATGAC 
ATTCAAGACC TCATCTATTT TGTCAGGTAC AAGCACTCCA CAGCAGAGGA GACAGCCACA 
CTGGTGATGG CACCACCTCT AGAGGAAGGC TTGGGTGGAG CAATGGAAGA GATGCAACCA 
CTGCATGAAG ATAATTTCTC ACGAGAGAAG ACAGCAGAAC TTAATGTGCA GGTTCCTGAA 
GAACCCACCC ACTTGGACCA ACGTGTGATT GGGGACACTC ATGCCTCAGA AGTGTCACAG 
AAGCCAAATA CTGAGAAAGA CCTGGACCCA GGGCCAGTTA CAACAGAAGA CACTCCTATG 
GATGCTATTG ATGCAAACAA GCAACCAGAG ACAGCCGCCG AAGAGCCGGC AAGTGTCACA 
CCTTTGGAAA ACGCAATCCT TCTAATATAT TCATTCATGT TTTATTTAAC TAAGTCGCTA 
GTTGCTACAT TGCCTGATGA TGTTCAGCCT GGGCCTGATT TTTATGGACT GCCATGGAAA 
CCTGTATTTA TCACTGCCTT CTTGGGAATT GCTTCGTTTG CCATTTTCTT ATGGAGAACT 
GTCCTTGTTG TGAAGGATAG AGTATATCAA GTCACGGAAC AGCAAATTTC TGAGAAGTTG 
AAGACTATCA TGAAAGAAAA TACAGAACTT GTACAAAAAT TGTCAAATTA TGAACAGAAG 
ATCAAGGAAT CAAAGAAACA TGTTCAGGAA ACCAGGAAAC AAAATATGAT TCTCTCTGAT 
GAAGCAATTA AATATAAGGA TAAAATCAAG ACACTTGAAA AAAATCAGGA AATTCTGGAT 
GACACAGCTA AAAATCTTCG TGTTATGCTA GAATCTGAGA GAGAACAGAA TGTCAAGAAT 
CAGGACTTGA TATCAGAAAA CAAGAAATCT ATAGAGAAGT TAAAGGATGT TATTTCAATG 
AATGCCTCAG AATTTTCAGA GGTTCAGATT GCACTTAATG AAGCTAAGCT TAGTGAAGAG 
AAGGTGAAGT CTGAATGCCA TCGGGTTCAA GAAGAAAATG CTAGGCTTAA GAAGAAAAAA 
GAGCAGTTGC AGCAGGAAAT CGAAGACTGG AGTAAATTAC ATGCTGAGCT CAGTGAGCAA 
ATCAAATCAT TTGAGAAGTC TCAGAAAGAT TTGGAAGTAG CTCTTACTCA CAAGGATGAT 
AATATTAATG CTTTGACTAA CTGCATTACA CAGTTGAATC TGTTAGAGTG TGAATCTGAA 
TCTGAGGGTC AAAATAAAGG TGGAAATGAT TCAGATGAAT TAGCAAATGG AGAAGTGGGA 
GGTGACCGGA ATGAGAAGAT GAAAAATCAA ATTAAGCAGA TGATGGATGT CTCTCGGACA 
CAGACTGCAA TATCGGTAGT TGAAGAGGAT CTAAAGCTTT TACAGCTTAA GCTAAGAGCC 
TCCGTGTCCA CTAAATGTAA CCTGGAAGAC CAGGTAAAGA AATTGGAAGA TGACCGCAAC 
TCACTACAAG CTGCCAAAGC TGGACTGGAA GATGAATGCA AAACCTTGAG GCAGAAAGTG 
GAGATTCTGA ATGAGCTCTA TCAGCAGAAG GAGATGGCTT TGCAAAAAAA ATTTGCAAGT 
ATCTTTTGCA TCAGAACTAT GAGTAAAGAA GTACAAGGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507815.3
CDS	10..4671
Translation	MAAAPGLLVWLLVLRLPWRVPGQLDPSTGRRFSEHKLCADDECSMLMYRGEALEDFTGPDCRFVNFKKGDPVYVYYKLARGWPEVWAGSVGRTFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFHNYNVEELLGFLELYNSAATDSEKAVEKTLQDMEKNPELSKEREPEPEPVEANSEESDSVFSENTEDLQEQFTTQKHHSHANSQANHAQGEQASFESFEEMLQDKLKVPESENNKTSNSSQVSNEQDKIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDTEYYAVGKEDEENQEDFDELPLLTFTDGEDMKTPAKSGVEKYPTDKEQNSNEEDKVQLTVPPGIKNDDKNILTTWGDTIFSIVTGGEETRDTMDLESSSSEEEKEDDDDALVPDSKQGKPQSATDYSDPDNVDDGLFIVDIPKTNNDKEVNAEHHIKGKGRGVQESKRGLVQDKTELEDENQEGMTVHSSVHSNNLNSMPAAEKGKDTLKSAYDDTENDLKGAAIHISKGMLHEEKPGEQILEGGSESESAQKAAGNQMNDRKIQQESLGSAPLMGDDHPNASRDSVEGDALVNGAKLHTLSVEHQREELKEELVLKTQNQPRFSSPDEIDLPRELEDEVPILGRNLPWQQERDVAATASKQMSEKIRLSEGEAKEDSLDEEFFHHKAMQGTEVGQTDQTDSTGGPAFLSKVEEDDYPSEELLEDENAINAKRSKEKNPGNQGRQFDVNLQVPDRAVLGTIHPDPEIEESKQETSMILDSEKTSETAAKGVNTGGREPNTMVEKERPLADKKAQRPFERSDFSDSIKIQTPELGEVFQNKDSDYLKNDNPEEHLKTSGLAGEPEGELSKEDHENTEKYMGTESQGSAAAEPEDDSFHWTPHTSVEPGHSDKREDLLIISSFFKEQQSLQRFQKYFNVHELEALLQEMSSKLKSAQQESLPYNMEKVLDKVFRASESQILSIAEKMLDTRVAENRDLGMNENNIFEEAAVLDDIQDLIYFVRYKHSTAEETATLVMAPPLEEGLGGAMEEMQPLHEDNFSREKTAELNVQVPEEPTHLDQRVIGDTHASEVSQKPNTEKDLDPGPVTTEDTPMDAIDANKQPETAAEEPASVTPLENAILLIYSFMFYLTKSLVATLPDDVQPGPDFYGLPWKPVFITAFLGIASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLISENKKSIEKLKDVISMNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKFASIFCIRTMSKEVQG

Target ORF information:

RefSeq Version	XM_011509513.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509513.3

ORF Insert Sequence:

ATGGCTGCGG CGCCTGGGCT GCTCGTCTGG CTGCTCGTGC TCCGGCTGCC CTGGCGGGTG 
CCGGGCCAGC TGGACCCCAG CACTGGCCGG CGGTTCTCGG AGCACAAACT CTGCGCGGAC 
GACGAATGCA GCATGTTAAT GTACCGCGGT GAGGCTCTTG AAGATTTCAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGTGAT CCTGTATATG TTTACTATAA ACTGGCAAGA 
GGATGGCCTG AAGTTTGGGC TGGAAGTGTT GGACGCACTT TTGGATATTT TCCAAAAGAT 
TTAATCCAGG TAGTTCATGA ATATACCAAA GAAGAGCTAC AAGTTCCAAC AGATGAGACG 
GATTTTGTTT GTTTTGATGG AGGAAGAGAT GATTTTCATA ATTATAATGT AGAAGAACTT 
TTAGGGTTTT TGGAACTGTA CAATTCTGCA GCTACAGATT CTGAGAAAGC TGTAGAAAAA 
ACTTTACAGG ATATGGAAAA AAACCCTGAA TTATCTAAGG AAAGGGAACC TGAACCTGAA 
CCAGTAGAAG CCAACTCAGA GGAAAGTGAT AGTGTATTCT CAGAAAACAC TGAGGATCTT 
CAGGAACAGT TTACAACTCA GAAGCACCAC TCCCATGCAA ACAGCCAAGC AAATCATGCT 
CAGGGAGAGC AGGCTTCATT TGAATCTTTT GAAGAAATGC TGCAAGATAA ACTAAAAGTG 
CCAGAAAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCAAATGA ACAGGATAAG 
ATTGATGCCT ATAAACTTTT GAAAAAAGAA ATGACTCTAG ACTTGAAAAC CAAATTTGGC 
TCAACAGCTG ATGCACTTGT ATCTGATGAT GAGACAACCA GACTCGTTAC TTCATTAGAA 
GATGATTTTG ATGAGGAATT GGATACTGAG TATTATGCAG TTGGAAAGGA AGATGAGGAG 
AACCAAGAAG ACTTTGATGA GTTGCCATTA CTTACCTTTA CAGATGGGGA AGATATGAAA 
ACTCCAGCAA AGTCTGGCGT TGAGAAATAT CCAACAGATA AAGAGCAGAA TTCAAATGAA 
GAGGACAAGG TTCAGCTAAC TGTGCCCCCT GGCATCAAAA ATGATGATAA AAATATACTA 
ACAACCTGGG GGGACACTAT CTTCTCTATT GTCACAGGAG GTGAAGAAAC AAGAGATACG 
ATGGATTTAG AGAGCTCTAG TTCAGAGGAA GAAAAAGAAG ATGATGATGA TGCATTAGTC 
CCAGATAGCA AACAGGGGAA ACCACAGTCA GCAACAGATT ATAGTGACCC TGACAATGTA 
GATGATGGTC TTTTTATTGT AGACATTCCT AAAACAAATA ATGACAAAGA AGTAAACGCA 
GAACATCACA TTAAAGGAAA AGGGAGGGGA GTTCAGGAAT CCAAGAGGGG CCTGGTACAA 
GATAAGACAG AATTAGAGGA TGAAAATCAA GAAGGCATGA CTGTGCACAG TTCTGTTCAC 
AGCAATAACC TCAACTCTAT GCCAGCTGCT GAAAAGGGTA AAGACACATT AAAATCAGCT 
TATGATGATA CAGAAAATGA CCTAAAAGGA GCAGCTATTC ATATCTCAAA AGGAATGCTC 
CACGAAGAAA AGCCTGGAGA GCAGATTTTG GAAGGTGGCT CAGAGAGTGA ATCTGCACAG 
AAAGCTGCAG GGAATCAAAT GAATGACAGA AAGATTCAAC AGGAATCCCT GGGTAGTGCA 
CCACTCATGG GAGATGACCA CCCTAACGCA TCCAGAGACA GTGTGGAGGG AGACGCTTTG 
GTAAATGGGG CCAAACTGCA CACGCTTTCA GTGGAGCATC AACGTGAGGA ATTGAAAGAG 
GAATTAGTTC TTAAAACTCA AAACCAACCT AGATTCTCCT CTCCAGATGA GATTGATTTG 
CCCAGAGAAC TGGAAGACGA GGTTCCCATT CTGGGAAGAA ATCTTCCCTG GCAACAAGAA 
AGAGATGTGG CTGCCACAGC CAGTAAGCAA ATGAGTGAGA AGATAAGGCT CTCTGAGGGA 
GAAGCCAAAG AGGACTCCTT GGATGAAGAG TTTTTTCATC ACAAGGCAAT GCAGGGCACA 
GAGGTAGGAC AGACAGACCA AACTGACAGC ACAGGAGGAC CAGCTTTCCT TTCTAAAGTA 
GAAGAGGATG ATTATCCCTC TGAAGAACTA CTAGAGGATG AAAACGCTAT AAATGCAAAA 
CGGTCTAAAG AAAAAAACCC TGGGAATCAG GGCAGGCAGT TTGATGTTAA TCTGCAAGTC 
CCTGACAGAG CAGTTTTAGG GACCATTCAT CCAGATCCAG AAATTGAAGA AAGCAAGCAA 
GAAACTAGTA TGATTTTGGA TAGCGAAAAA ACAAGTGAGA CTGCTGCCAA AGGGGTCAAC 
ACAGGAGGCA GGGAACCAAA TACAATGGTG GAAAAAGAAC GCCCTCTGGC AGATAAGAAA 
GCACAGAGAC CATTTGAACG AAGTGACTTT TCTGACAGCA TAAAAATTCA GACTCCAGAA 
TTAGGTGAAG TGTTTCAGAA TAAAGATTCT GATTATCTGA AGAACGACAA CCCTGAGGAA 
CATCTGAAGA CCTCAGGGCT TGCAGGGGAG CCTGAGGGAG AACTCTCAAA AGAGGACCAT 
GAGAACACAG AGAAGTACAT GGGCACAGAA AGCCAGGGGT CTGCTGCTGC AGAACCTGAA 
GATGACTCGT TCCACTGGAC TCCACATACA AGTGTAGAGC CAGGGCATAG TGACAAGAGG 
GAGGACTTAC TTATCATAAG CAGCTTCTTT AAAGAACAAC AGTCTTTGCA GCGGTTCCAG 
AAGTACTTTA ATGTCCATGA GCTGGAAGCC TTGCTACAAG AAATGTCATC AAAACTGAAG 
TCAGCGCAGC AGGAGAGCCT GCCCTATAAT ATGGAAAAAG TCCTAGATAA GGTCTTCCGT 
GCTTCTGAGT CACAAATTCT GAGCATAGCA GAAAAAATGC TTGATACTCG TGTGGCTGAA 
AATAGAGATC TGGGAATGAA CGAAAATAAC ATATTTGAAG AGGCTGCAGT GCTTGATGAC 
ATTCAAGACC TCATCTATTT TGTCAGGTAC AAGCACTCCA CAGCAGAGGA GACAGCCACA 
CTGGTGATGG CACCACCTCT AGAGGAAGGC TTGGGTGGAG CAATGGAAGA GATGCAACCA 
CTGCATGAAG ATAATTTCTC ACGAGAGAAG ACAGCAGAAC TTAATGTGCA GGTTCCTGAA 
GAACCCACCC ACTTGGACCA ACGTGTGATT GGGGACACTC ATGCCTCAGA AGTGTCACAG 
AAGCCAAATA CTGAGAAAGA CCTGGACCCA GGGCCAGTTA CAACAGAAGA CACTCCTATG 
GATGCTATTG ATGCAAACAA GCAACCAGAG ACAGCCGCCG AAGAGCCGGC AAGTGTCACA 
CCTTTGGAAA ACGCAATCCT TCTAATATAT TCATTCATGT TTTATTTAAC TAAGTCGCTA 
GTTGCTACAT TGCCTGATGA TGTTCAGCCT GGGCCTGATT TTTATGGACT GCCATGGAAA 
CCTGTATTTA TCACTGCCTT CTTGGGAATT GCTTCGTTTG CCATTTTCTT ATGGAGAACT 
GTCCTTGTTG TGAAGGATAG AGTATATCAA GTCACGGAAC AGCAAATTTC TGAGAAGTTG 
AAGACTATCA TGAAAGAAAA TACAGAACTT GTACAAAAAT TGTCAAATTA TGAACAGAAG 
ATCAAGGAAT CAAAGAAACA TGTTCAGGAA ACCAGGAAAC AAAATATGAT TCTCTCTGAT 
GAAGCAATTA AATATAAGGA TAAAATCAAG ACACTTGAAA AAAATCAGGA AATTCTGGAT 
GACACAGCTA AAAATCTTCG TGTTATGCTA GAATCTGAGA GAGAACAGAA TGTCAAGAAT 
CAGGACTTGA TATCAGAAAA CAAGAAATCT ATAGAGAAGT TAAAGGATGT TATTTCAATG 
AATGCCTCAG AATTTTCAGA GGTTCAGATT GCACTTAATG AAGCTAAGCT TAGTGAAGAG 
AAGGTGAAGT CTGAATGCCA TCGGGTTCAA GAAGAAAATG CTAGGCTTAA GAAGAAAAAA 
GAGCAGTTGC AGCAGGAAAT CGAAGACTGG AGTAAATTAC ATGCTGAGCT CAGTGAGCAA 
ATCAAATCAT TTGAGAAGTC TCAGAAAGAT TTGGAAGTAG CTCTTACTCA CAAGGATGAT 
AATATTAATG CTTTGACTAA CTGCATTACA CAGTTGAATC TGTTAGAGTG TGAATCTGAA 
TCTGAGGGTC AAAATAAAGG TGGAAATGAT TCAGATGAAT TAGCAAATGG AGAAGTGGGA 
GGTGACCGGA ATGAGAAGAT GAAAAATCAA ATTAAGCAGA TGATGGATGT CTCTCGGACA 
CAGACTGCAA TATCGGTAGT TGAAGAGGAT CTAAAGCTTT TACAGCTTAA GCTAAGAGCC 
TCCGTGTCCA CTAAATGTAA CCTGGAAGAC CAGGTAAAGA AATTGGAAGA TGACCGCAAC 
TCACTACAAG CTGCCAAAGC TGGACTGGAA GATGAATGCA AAACCTTGAG GCAGAAAGTG 
GAGATTCTGA ATGAGCTCTA TCAGCAGAAG GAGATGGCTT TGCAAAAAAA ATTTGCAAGT 
ATCTTTTGCA TCAGAACTAT GAGTAAAGAA GTACAAGGGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_017001243.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856732.1 Homo sapiens melanoma inhibitory activity family member 3 (MIA3), transcript variant X2, mRNA.

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CloneID	OHu87371
Clone ID Related Accession (Same CDS sequence)	XM_017001243.1 , XM_017001243.2
Accession Version	XM_017001243.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	melanoma inhibitory activity protein 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAA ACCCTGAATT ATCTAAGGAA AGGGAACCTG AACCTGAACC AGTAGAAGCC 
AACTCAGAGG AAAGTGATAG TGTATTCTCA GAAAACACTG AGGATCTTCA GGAACAGTTT 
ACAACTCAGA AGCACCACTC CCATGCAAAC AGCCAAGCAA ATCATGCTCA GGGAGAGCAG 
GCTTCATTTG AATCTTTTGA AGAAATGCTG CAAGATAAAC TAAAAGTGCC AGAAAGTGAA 
AACAACAAAA CCAGCAATAG TTCTCAGGTC TCAAATGAAC AGGATAAGAT TGATGCCTAT 
AAACTTTTGA AAAAAGAAAT GACTCTAGAC TTGAAAACCA AATTTGGCTC AACAGCTGAT 
GCACTTGTAT CTGATGATGA GACAACCAGA CTCGTTACTT CATTAGAAGA TGATTTTGAT 
GAGGAATTGG ATACTGAGTA TTATGCAGTT GGAAAGGAAG ATGAGGAGAA CCAAGAAGAC 
TTTGATGAGT TGCCATTACT TACCTTTACA GATGGGGAAG ATATGAAAAC TCCAGCAAAG 
TCTGGCGTTG AGAAATATCC AACAGATAAA GAGCAGAATT CAAATGAAGA GGACAAGGTT 
CAGCTAACTG TGCCCCCTGG CATCAAAAAT GATGATAAAA ATATACTAAC AACCTGGGGG 
GACACTATCT TCTCTATTGT CACAGGAGGT GAAGAAACAA GAGATACGAT GGATTTAGAG 
AGCTCTAGTT CAGAGGAAGA AAAAGAAGAT GATGATGATG CATTAGTCCC AGATAGCAAA 
CAGGGGAAAC CACAGTCAGC AACAGATTAT AGTGACCCTG ACAATGTAGA TGATGGTCTT 
TTTATTGTAG ACATTCCTAA AACAAATAAT GACAAAGAAG TAAACGCAGA ACATCACATT 
AAAGGAAAAG GGAGGGGAGT TCAGGAATCC AAGAGGGGCC TGGTACAAGA TAAGACAGAA 
TTAGAGGATG AAAATCAAGA AGGCATGACT GTGCACAGTT CTGTTCACAG CAATAACCTC 
AACTCTATGC CAGCTGCTGA AAAGGGTAAA GACACATTAA AATCAGCTTA TGATGATACA 
GAAAATGACC TAAAAGGAGC AGCTATTCAT ATCTCAAAAG GAATGCTCCA CGAAGAAAAG 
CCTGGAGAGC AGATTTTGGA AGGTGGCTCA GAGAGTGAAT CTGCACAGAA AGCTGCAGGG 
AATCAAATGA ATGACAGAAA GATTCAACAG GAATCCCTGG GTAGTGCACC ACTCATGGGA 
GATGACCACC CTAACGCATC CAGAGACAGT GTGGAGGGAG ACGCTTTGGT AAATGGGGCC 
AAACTGCACA CGCTTTCAGT GGAGCATCAA CGTGAGGAAT TGAAAGAGGA ATTAGTTCTT 
AAAACTCAAA ACCAACCTAG ATTCTCCTCT CCAGATGAGA TTGATTTGCC CAGAGAACTG 
GAAGACGAGG TTCCCATTCT GGGAAGAAAT CTTCCCTGGC AACAAGAAAG AGATGTGGCT 
GCCACAGCCA GTAAGCAAAT GAGTGAGAAG ATAAGGCTCT CTGAGGGAGA AGCCAAAGAG 
GACTCCTTGG ATGAAGAGTT TTTTCATCAC AAGGCAATGC AGGGCACAGA GGTAGGACAG 
ACAGACCAAA CTGACAGCAC AGGAGGACCA GCTTTCCTTT CTAAAGTAGA AGAGGATGAT 
TATCCCTCTG AAGAACTACT AGAGGATGAA AACGCTATAA ATGCAAAACG GTCTAAAGAA 
AAAAACCCTG GGAATCAGGG CAGGCAGTTT GATGTTAATC TGCAAGTCCC TGACAGAGCA 
GTTTTAGGGA CCATTCATCC AGATCCAGAA ATTGAAGAAA GCAAGCAAGA AACTAGTATG 
ATTTTGGATA GCGAAAAAAC AAGTGAGACT GCTGCCAAAG GGGTCAACAC AGGAGGCAGG 
GAACCAAATA CAATGGTGGA AAAAGAACGC CCTCTGGCAG ATAAGAAAGC ACAGAGACCA 
TTTGAACGAA GTGACTTTTC TGACAGCATA AAAATTCAGA CTCCAGAATT AGGTGAAGTG 
TTTCAGAATA AAGATTCTGA TTATCTGAAG AACGACAACC CTGAGGAACA TCTGAAGACC 
TCAGGGCTTG CAGGGGAGCC TGAGGGAGAA CTCTCAAAAG AGGACCATGA GAACACAGAG 
AAGTACATGG GCACAGAAAG CCAGGGGTCT GCTGCTGCAG AACCTGAAGA TGACTCGTTC 
CACTGGACTC CACATACAAG TGTAGAGCCA GGGCATAGTG ACAAGAGGGA GGACTTACTT 
ATCATAAGCA GCTTCTTTAA AGAACAACAG TCTTTGCAGC GGTTCCAGAA GTACTTTAAT 
GTCCATGAGC TGGAAGCCTT GCTACAAGAA ATGTCATCAA AACTGAAGTC AGCGCAGCAG 
GAGAGCCTGC CCTATAATAT GGAAAAAGTC CTAGATAAGG TCTTCCGTGC TTCTGAGTCA 
CAAATTCTGA GCATAGCAGA AAAAATGCTT GATACTCGTG TGGCTGAAAA TAGAGATCTG 
GGAATGAACG AAAATAACAT ATTTGAAGAG GCTGCAGTGC TTGATGACAT TCAAGACCTC 
ATCTATTTTG TCAGGTACAA GCACTCCACA GCAGAGGAGA CAGCCACACT GGTGATGGCA 
CCACCTCTAG AGGAAGGCTT GGGTGGAGCA ATGGAAGAGA TGCAACCACT GCATGAAGAT 
AATTTCTCAC GAGAGAAGAC AGCAGAACTT AATGTGCAGG TTCCTGAAGA ACCCACCCAC 
TTGGACCAAC GTGTGATTGG GGACACTCAT GCCTCAGAAG TGTCACAGAA GCCAAATACT 
GAGAAAGACC TGGACCCAGG GCCAGTTACA ACAGAAGACA CTCCTATGGA TGCTATTGAT 
GCAAACAAGC AACCAGAGAC AGCCGCCGAA GAGCCGGCAA GTGTCACACC TTTGGAAAAC 
GCAATCCTTC TAATATATTC ATTCATGTTT TATTTAACTA AGTCGCTAGT TGCTACATTG 
CCTGATGATG TTCAGCCTGG GCCTGATTTT TATGGACTGC CATGGAAACC TGTATTTATC 
ACTGCCTTCT TGGGAATTGC TTCGTTTGCC ATTTTCTTAT GGAGAACTGT CCTTGTTGTG 
AAGGATAGAG TATATCAAGT CACGGAACAG CAAATTTCTG AGAAGTTGAA GACTATCATG 
AAAGAAAATA CAGAACTTGT ACAAAAATTG TCAAATTATG AACAGAAGAT CAAGGAATCA 
AAGAAACATG TTCAGGAAAC CAGGAAACAA AATATGATTC TCTCTGATGA AGCAATTAAA 
TATAAGGATA AAATCAAGAC ACTTGAAAAA AATCAGGAAA TTCTGGATGA CACAGCTAAA 
AATCTTCGTG TTATGCTAGA ATCTGAGAGA GAACAGAATG TCAAGAATCA GGACTTGATA 
TCAGAAAACA AGAAATCTAT AGAGAAGTTA AAGGATGTTA TTTCAATGAA TGCCTCAGAA 
TTTTCAGAGG TTCAGATTGC ACTTAATGAA GCTAAGCTTA GTGAAGAGAA GGTGAAGTCT 
GAATGCCATC GGGTTCAAGA AGAAAATGCT AGGCTTAAGA AGAAAAAAGA GCAGTTGCAG 
CAGGAAATCG AAGACTGGAG TAAATTACAT GCTGAGCTCA GTGAGCAAAT CAAATCATTT 
GAGAAGTCTC AGAAAGATTT GGAAGTAGCT CTTACTCACA AGGATGATAA TATTAATGCT 
TTGACTAACT GCATTACACA GTTGAATCTG TTAGAGTGTG AATCTGAATC TGAGGGTCAA 
AATAAAGGTG GAAATGATTC AGATGAATTA GCAAATGGAG AAGTGGGAGG TGACCGGAAT 
GAGAAGATGA AAAATCAAAT TAAGCAGATG ATGGATGTCT CTCGGACACA GACTGCAATA 
TCGGTAGTTG AAGAGGATCT AAAGCTTTTA CAGCTTAAGC TAAGAGCCTC CGTGTCCACT 
AAATGTAACC TGGAAGACCA GGTAAAGAAA TTGGAAGATG ACCGCAACTC ACTACAAGCT 
GCCAAAGCTG GACTGGAAGA TGAATGCAAA ACCTTGAGGC AGAAAGTGGA GATTCTGAAT 
GAGCTCTATC AGCAGAAGGA GATGGCTTTG CAAAAGAAAC TGAGTCAAGA AGAGTATGAA 
CGGCAAGAAA GAGAGCACAG GCTGTCAGCT GCAGATGAAA AGGCAGTTTC GGCTGCAGAG 
GAAGTAAAAA CTTACAAGCG GAGAATTGAA GAAATGGAGG ATGAATTACA GAAGACAGAG 
CGGTCATTTA AAAACCAGAT CGCTACCCAT GAGAAGAAAG CTCATGAAAA CTGGCTCAAA 
GCTCGTGCTG CAGAAAGAGC TATAGCTGAA GAGAAAAGGG AAGCTGCCAA TTTGAGACAC 
AAATTATTAG AATTAACACA AAAGATGGCA ATGCTGCAAG AAGAACCTGT GATTGTAAAA 
CCAATGCCAG GAAAACCAAA TACACAAAAC CCTCCACGGA GAGGTCCTCT GAGCCAGAAT 
GGCTCTTTTG GCCCATCCCC TGTGAGTGGT GGAGAATGCT CCCCTCCATT GACAGTGGAG 
CCACCCGTGA GACCTCTCTC TGCTACTCTC AATCGAAGAG ATATGCCTAG AAGTGAATTT 
GGATCAGTGG ACGGGCCTCT ACCTCATCCT CGATGGTCAG CTGAGGCATC TGGGAAACCC 
TCTCCTTCTG ATCCAGGATC TGGTACAGCT ACCATGATGA ACAGCAGCTC AAGAGGCTCT 
TCCCCTACCA GGGTACTCGA TGAAGGCAAG CAAACTGTTC TCCAAGAGCC TGAAGTCCCC 
TCAGTTCCCA GCATTACATC TTTGGCTGAG CGTCCAGTAG CAGTTAATAT GGCTCCAAAA 
GGGCCCCCTC CTTTCCCAGG AGTCCCTCTC ATGAGCACCC CCATGGGAGG CCCTGTACCA 
CCACCCATTC GATATGGACC ACCACCTCAG CTCTGCGGAC CTTTTGGGCC TCGGCCACTT 
CCTCCACCCT TTGGCCCTGG TATGCGTCCA CCACTAGGCT TAAGAGAATT TGCACCAGGC 
GTTCCACCAG GAAGACGGGA CCTGCCTCTC CACCCTCGGG GATTTTTACC TGGACACGCA 
CCATTTAGAC CTTTAGGTTC ACTTGGCCCA AGAGAGTACT TTATTCCTGG TACCCGATTA 
CCACCCCCAA CCCATGGTCC CCAGGAATAC CCACCACCAC CTGCTGTAAG AGACTTACTG 
CCGTCAGGCT CTAGAGATGA GCCTCCACCT GCCTCTCAGA GCACTAGCCA GGACTGTTCA 
CAGGCTTTAA AACAGAGCCC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856732.1
CDS	327..5630
Translation	MEKNPELSKEREPEPEPVEANSEESDSVFSENTEDLQEQFTTQKHHSHANSQANHAQGEQASFESFEEMLQDKLKVPESENNKTSNSSQVSNEQDKIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDTEYYAVGKEDEENQEDFDELPLLTFTDGEDMKTPAKSGVEKYPTDKEQNSNEEDKVQLTVPPGIKNDDKNILTTWGDTIFSIVTGGEETRDTMDLESSSSEEEKEDDDDALVPDSKQGKPQSATDYSDPDNVDDGLFIVDIPKTNNDKEVNAEHHIKGKGRGVQESKRGLVQDKTELEDENQEGMTVHSSVHSNNLNSMPAAEKGKDTLKSAYDDTENDLKGAAIHISKGMLHEEKPGEQILEGGSESESAQKAAGNQMNDRKIQQESLGSAPLMGDDHPNASRDSVEGDALVNGAKLHTLSVEHQREELKEELVLKTQNQPRFSSPDEIDLPRELEDEVPILGRNLPWQQERDVAATASKQMSEKIRLSEGEAKEDSLDEEFFHHKAMQGTEVGQTDQTDSTGGPAFLSKVEEDDYPSEELLEDENAINAKRSKEKNPGNQGRQFDVNLQVPDRAVLGTIHPDPEIEESKQETSMILDSEKTSETAAKGVNTGGREPNTMVEKERPLADKKAQRPFERSDFSDSIKIQTPELGEVFQNKDSDYLKNDNPEEHLKTSGLAGEPEGELSKEDHENTEKYMGTESQGSAAAEPEDDSFHWTPHTSVEPGHSDKREDLLIISSFFKEQQSLQRFQKYFNVHELEALLQEMSSKLKSAQQESLPYNMEKVLDKVFRASESQILSIAEKMLDTRVAENRDLGMNENNIFEEAAVLDDIQDLIYFVRYKHSTAEETATLVMAPPLEEGLGGAMEEMQPLHEDNFSREKTAELNVQVPEEPTHLDQRVIGDTHASEVSQKPNTEKDLDPGPVTTEDTPMDAIDANKQPETAAEEPASVTPLENAILLIYSFMFYLTKSLVATLPDDVQPGPDFYGLPWKPVFITAFLGIASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLISENKKSIEKLKDVISMNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREHRLSAADEKAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGKPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKQTVLQEPEVPSVPSITSLAERPVAVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_017001243.1
Organism	Homo sapiens(human)
Definition	Homo sapiens melanoma inhibitory activity family member 3 (MIA3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001243.1

ORF Insert Sequence:

ATGGAAAAAA ACCCTGAATT ATCTAAGGAA AGGGAACCTG AACCTGAACC AGTAGAAGCC 
AACTCAGAGG AAAGTGATAG TGTATTCTCA GAAAACACTG AGGATCTTCA GGAACAGTTT 
ACAACTCAGA AGCACCACTC CCATGCAAAC AGCCAAGCAA ATCATGCTCA GGGAGAGCAG 
GCTTCATTTG AATCTTTTGA AGAAATGCTG CAAGATAAAC TAAAAGTGCC AGAAAGTGAA 
AACAACAAAA CCAGCAATAG TTCTCAGGTC TCAAATGAAC AGGATAAGAT TGATGCCTAT 
AAACTTTTGA AAAAAGAAAT GACTCTAGAC TTGAAAACCA AATTTGGCTC AACAGCTGAT 
GCACTTGTAT CTGATGATGA GACAACCAGA CTCGTTACTT CATTAGAAGA TGATTTTGAT 
GAGGAATTGG ATACTGAGTA TTATGCAGTT GGAAAGGAAG ATGAGGAGAA CCAAGAAGAC 
TTTGATGAGT TGCCATTACT TACCTTTACA GATGGGGAAG ATATGAAAAC TCCAGCAAAG 
TCTGGCGTTG AGAAATATCC AACAGATAAA GAGCAGAATT CAAATGAAGA GGACAAGGTT 
CAGCTAACTG TGCCCCCTGG CATCAAAAAT GATGATAAAA ATATACTAAC AACCTGGGGG 
GACACTATCT TCTCTATTGT CACAGGAGGT GAAGAAACAA GAGATACGAT GGATTTAGAG 
AGCTCTAGTT CAGAGGAAGA AAAAGAAGAT GATGATGATG CATTAGTCCC AGATAGCAAA 
CAGGGGAAAC CACAGTCAGC AACAGATTAT AGTGACCCTG ACAATGTAGA TGATGGTCTT 
TTTATTGTAG ACATTCCTAA AACAAATAAT GACAAAGAAG TAAACGCAGA ACATCACATT 
AAAGGAAAAG GGAGGGGAGT TCAGGAATCC AAGAGGGGCC TGGTACAAGA TAAGACAGAA 
TTAGAGGATG AAAATCAAGA AGGCATGACT GTGCACAGTT CTGTTCACAG CAATAACCTC 
AACTCTATGC CAGCTGCTGA AAAGGGTAAA GACACATTAA AATCAGCTTA TGATGATACA 
GAAAATGACC TAAAAGGAGC AGCTATTCAT ATCTCAAAAG GAATGCTCCA CGAAGAAAAG 
CCTGGAGAGC AGATTTTGGA AGGTGGCTCA GAGAGTGAAT CTGCACAGAA AGCTGCAGGG 
AATCAAATGA ATGACAGAAA GATTCAACAG GAATCCCTGG GTAGTGCACC ACTCATGGGA 
GATGACCACC CTAACGCATC CAGAGACAGT GTGGAGGGAG ACGCTTTGGT AAATGGGGCC 
AAACTGCACA CGCTTTCAGT GGAGCATCAA CGTGAGGAAT TGAAAGAGGA ATTAGTTCTT 
AAAACTCAAA ACCAACCTAG ATTCTCCTCT CCAGATGAGA TTGATTTGCC CAGAGAACTG 
GAAGACGAGG TTCCCATTCT GGGAAGAAAT CTTCCCTGGC AACAAGAAAG AGATGTGGCT 
GCCACAGCCA GTAAGCAAAT GAGTGAGAAG ATAAGGCTCT CTGAGGGAGA AGCCAAAGAG 
GACTCCTTGG ATGAAGAGTT TTTTCATCAC AAGGCAATGC AGGGCACAGA GGTAGGACAG 
ACAGACCAAA CTGACAGCAC AGGAGGACCA GCTTTCCTTT CTAAAGTAGA AGAGGATGAT 
TATCCCTCTG AAGAACTACT AGAGGATGAA AACGCTATAA ATGCAAAACG GTCTAAAGAA 
AAAAACCCTG GGAATCAGGG CAGGCAGTTT GATGTTAATC TGCAAGTCCC TGACAGAGCA 
GTTTTAGGGA CCATTCATCC AGATCCAGAA ATTGAAGAAA GCAAGCAAGA AACTAGTATG 
ATTTTGGATA GCGAAAAAAC AAGTGAGACT GCTGCCAAAG GGGTCAACAC AGGAGGCAGG 
GAACCAAATA CAATGGTGGA AAAAGAACGC CCTCTGGCAG ATAAGAAAGC ACAGAGACCA 
TTTGAACGAA GTGACTTTTC TGACAGCATA AAAATTCAGA CTCCAGAATT AGGTGAAGTG 
TTTCAGAATA AAGATTCTGA TTATCTGAAG AACGACAACC CTGAGGAACA TCTGAAGACC 
TCAGGGCTTG CAGGGGAGCC TGAGGGAGAA CTCTCAAAAG AGGACCATGA GAACACAGAG 
AAGTACATGG GCACAGAAAG CCAGGGGTCT GCTGCTGCAG AACCTGAAGA TGACTCGTTC 
CACTGGACTC CACATACAAG TGTAGAGCCA GGGCATAGTG ACAAGAGGGA GGACTTACTT 
ATCATAAGCA GCTTCTTTAA AGAACAACAG TCTTTGCAGC GGTTCCAGAA GTACTTTAAT 
GTCCATGAGC TGGAAGCCTT GCTACAAGAA ATGTCATCAA AACTGAAGTC AGCGCAGCAG 
GAGAGCCTGC CCTATAATAT GGAAAAAGTC CTAGATAAGG TCTTCCGTGC TTCTGAGTCA 
CAAATTCTGA GCATAGCAGA AAAAATGCTT GATACTCGTG TGGCTGAAAA TAGAGATCTG 
GGAATGAACG AAAATAACAT ATTTGAAGAG GCTGCAGTGC TTGATGACAT TCAAGACCTC 
ATCTATTTTG TCAGGTACAA GCACTCCACA GCAGAGGAGA CAGCCACACT GGTGATGGCA 
CCACCTCTAG AGGAAGGCTT GGGTGGAGCA ATGGAAGAGA TGCAACCACT GCATGAAGAT 
AATTTCTCAC GAGAGAAGAC AGCAGAACTT AATGTGCAGG TTCCTGAAGA ACCCACCCAC 
TTGGACCAAC GTGTGATTGG GGACACTCAT GCCTCAGAAG TGTCACAGAA GCCAAATACT 
GAGAAAGACC TGGACCCAGG GCCAGTTACA ACAGAAGACA CTCCTATGGA TGCTATTGAT 
GCAAACAAGC AACCAGAGAC AGCCGCCGAA GAGCCGGCAA GTGTCACACC TTTGGAAAAC 
GCAATCCTTC TAATATATTC ATTCATGTTT TATTTAACTA AGTCGCTAGT TGCTACATTG 
CCTGATGATG TTCAGCCTGG GCCTGATTTT TATGGACTGC CATGGAAACC TGTATTTATC 
ACTGCCTTCT TGGGAATTGC TTCGTTTGCC ATTTTCTTAT GGAGAACTGT CCTTGTTGTG 
AAGGATAGAG TATATCAAGT CACGGAACAG CAAATTTCTG AGAAGTTGAA GACTATCATG 
AAAGAAAATA CAGAACTTGT ACAAAAATTG TCAAATTATG AACAGAAGAT CAAGGAATCA 
AAGAAACATG TTCAGGAAAC CAGGAAACAA AATATGATTC TCTCTGATGA AGCAATTAAA 
TATAAGGATA AAATCAAGAC ACTTGAAAAA AATCAGGAAA TTCTGGATGA CACAGCTAAA 
AATCTTCGTG TTATGCTAGA ATCTGAGAGA GAACAGAATG TCAAGAATCA GGACTTGATA 
TCAGAAAACA AGAAATCTAT AGAGAAGTTA AAGGATGTTA TTTCAATGAA TGCCTCAGAA 
TTTTCAGAGG TTCAGATTGC ACTTAATGAA GCTAAGCTTA GTGAAGAGAA GGTGAAGTCT 
GAATGCCATC GGGTTCAAGA AGAAAATGCT AGGCTTAAGA AGAAAAAAGA GCAGTTGCAG 
CAGGAAATCG AAGACTGGAG TAAATTACAT GCTGAGCTCA GTGAGCAAAT CAAATCATTT 
GAGAAGTCTC AGAAAGATTT GGAAGTAGCT CTTACTCACA AGGATGATAA TATTAATGCT 
TTGACTAACT GCATTACACA GTTGAATCTG TTAGAGTGTG AATCTGAATC TGAGGGTCAA 
AATAAAGGTG GAAATGATTC AGATGAATTA GCAAATGGAG AAGTGGGAGG TGACCGGAAT 
GAGAAGATGA AAAATCAAAT TAAGCAGATG ATGGATGTCT CTCGGACACA GACTGCAATA 
TCGGTAGTTG AAGAGGATCT AAAGCTTTTA CAGCTTAAGC TAAGAGCCTC CGTGTCCACT 
AAATGTAACC TGGAAGACCA GGTAAAGAAA TTGGAAGATG ACCGCAACTC ACTACAAGCT 
GCCAAAGCTG GACTGGAAGA TGAATGCAAA ACCTTGAGGC AGAAAGTGGA GATTCTGAAT 
GAGCTCTATC AGCAGAAGGA GATGGCTTTG CAAAAGAAAC TGAGTCAAGA AGAGTATGAA 
CGGCAAGAAA GAGAGCACAG GCTGTCAGCT GCAGATGAAA AGGCAGTTTC GGCTGCAGAG 
GAAGTAAAAA CTTACAAGCG GAGAATTGAA GAAATGGAGG ATGAATTACA GAAGACAGAG 
CGGTCATTTA AAAACCAGAT CGCTACCCAT GAGAAGAAAG CTCATGAAAA CTGGCTCAAA 
GCTCGTGCTG CAGAAAGAGC TATAGCTGAA GAGAAAAGGG AAGCTGCCAA TTTGAGACAC 
AAATTATTAG AATTAACACA AAAGATGGCA ATGCTGCAAG AAGAACCTGT GATTGTAAAA 
CCAATGCCAG GAAAACCAAA TACACAAAAC CCTCCACGGA GAGGTCCTCT GAGCCAGAAT 
GGCTCTTTTG GCCCATCCCC TGTGAGTGGT GGAGAATGCT CCCCTCCATT GACAGTGGAG 
CCACCCGTGA GACCTCTCTC TGCTACTCTC AATCGAAGAG ATATGCCTAG AAGTGAATTT 
GGATCAGTGG ACGGGCCTCT ACCTCATCCT CGATGGTCAG CTGAGGCATC TGGGAAACCC 
TCTCCTTCTG ATCCAGGATC TGGTACAGCT ACCATGATGA ACAGCAGCTC AAGAGGCTCT 
TCCCCTACCA GGGTACTCGA TGAAGGCAAG CAAACTGTTC TCCAAGAGCC TGAAGTCCCC 
TCAGTTCCCA GCATTACATC TTTGGCTGAG CGTCCAGTAG CAGTTAATAT GGCTCCAAAA 
GGGCCCCCTC CTTTCCCAGG AGTCCCTCTC ATGAGCACCC CCATGGGAGG CCCTGTACCA 
CCACCCATTC GATATGGACC ACCACCTCAG CTCTGCGGAC CTTTTGGGCC TCGGCCACTT 
CCTCCACCCT TTGGCCCTGG TATGCGTCCA CCACTAGGCT TAAGAGAATT TGCACCAGGC 
GTTCCACCAG GAAGACGGGA CCTGCCTCTC CACCCTCGGG GATTTTTACC TGGACACGCA 
CCATTTAGAC CTTTAGGTTC ACTTGGCCCA AGAGAGTACT TTATTCCTGG TACCCGATTA 
CCACCCCCAA CCCATGGTCC CCAGGAATAC CCACCACCAC CTGCTGTAAG AGACTTACTG 
CCGTCAGGCT CTAGAGATGA GCCTCCACCT GCCTCTCAGA GCACTAGCCA GGACTGTTCA 
CAGGCTTTAA AACAGAGCCC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_017001243.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856732.1 Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant X2, mRNA.

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CloneID	OHu87371
Clone ID Related Accession (Same CDS sequence)	XM_017001243.1 , XM_017001243.2
Accession Version	XM_017001243.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	transport and Golgi organization protein 1 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017001243.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAA ACCCTGAATT ATCTAAGGAA AGGGAACCTG AACCTGAACC AGTAGAAGCC 
AACTCAGAGG AAAGTGATAG TGTATTCTCA GAAAACACTG AGGATCTTCA GGAACAGTTT 
ACAACTCAGA AGCACCACTC CCATGCAAAC AGCCAAGCAA ATCATGCTCA GGGAGAGCAG 
GCTTCATTTG AATCTTTTGA AGAAATGCTG CAAGATAAAC TAAAAGTGCC AGAAAGTGAA 
AACAACAAAA CCAGCAATAG TTCTCAGGTC TCAAATGAAC AGGATAAGAT TGATGCCTAT 
AAACTTTTGA AAAAAGAAAT GACTCTAGAC TTGAAAACCA AATTTGGCTC AACAGCTGAT 
GCACTTGTAT CTGATGATGA GACAACCAGA CTCGTTACTT CATTAGAAGA TGATTTTGAT 
GAGGAATTGG ATACTGAGTA TTATGCAGTT GGAAAGGAAG ATGAGGAGAA CCAAGAAGAC 
TTTGATGAGT TGCCATTACT TACCTTTACA GATGGGGAAG ATATGAAAAC TCCAGCAAAG 
TCTGGCGTTG AGAAATATCC AACAGATAAA GAGCAGAATT CAAATGAAGA GGACAAGGTT 
CAGCTAACTG TGCCCCCTGG CATCAAAAAT GATGATAAAA ATATACTAAC AACCTGGGGG 
GACACTATCT TCTCTATTGT CACAGGAGGT GAAGAAACAA GAGATACGAT GGATTTAGAG 
AGCTCTAGTT CAGAGGAAGA AAAAGAAGAT GATGATGATG CATTAGTCCC AGATAGCAAA 
CAGGGGAAAC CACAGTCAGC AACAGATTAT AGTGACCCTG ACAATGTAGA TGATGGTCTT 
TTTATTGTAG ACATTCCTAA AACAAATAAT GACAAAGAAG TAAACGCAGA ACATCACATT 
AAAGGAAAAG GGAGGGGAGT TCAGGAATCC AAGAGGGGCC TGGTACAAGA TAAGACAGAA 
TTAGAGGATG AAAATCAAGA AGGCATGACT GTGCACAGTT CTGTTCACAG CAATAACCTC 
AACTCTATGC CAGCTGCTGA AAAGGGTAAA GACACATTAA AATCAGCTTA TGATGATACA 
GAAAATGACC TAAAAGGAGC AGCTATTCAT ATCTCAAAAG GAATGCTCCA CGAAGAAAAG 
CCTGGAGAGC AGATTTTGGA AGGTGGCTCA GAGAGTGAAT CTGCACAGAA AGCTGCAGGG 
AATCAAATGA ATGACAGAAA GATTCAACAG GAATCCCTGG GTAGTGCACC ACTCATGGGA 
GATGACCACC CTAACGCATC CAGAGACAGT GTGGAGGGAG ACGCTTTGGT AAATGGGGCC 
AAACTGCACA CGCTTTCAGT GGAGCATCAA CGTGAGGAAT TGAAAGAGGA ATTAGTTCTT 
AAAACTCAAA ACCAACCTAG ATTCTCCTCT CCAGATGAGA TTGATTTGCC CAGAGAACTG 
GAAGACGAGG TTCCCATTCT GGGAAGAAAT CTTCCCTGGC AACAAGAAAG AGATGTGGCT 
GCCACAGCCA GTAAGCAAAT GAGTGAGAAG ATAAGGCTCT CTGAGGGAGA AGCCAAAGAG 
GACTCCTTGG ATGAAGAGTT TTTTCATCAC AAGGCAATGC AGGGCACAGA GGTAGGACAG 
ACAGACCAAA CTGACAGCAC AGGAGGACCA GCTTTCCTTT CTAAAGTAGA AGAGGATGAT 
TATCCCTCTG AAGAACTACT AGAGGATGAA AACGCTATAA ATGCAAAACG GTCTAAAGAA 
AAAAACCCTG GGAATCAGGG CAGGCAGTTT GATGTTAATC TGCAAGTCCC TGACAGAGCA 
GTTTTAGGGA CCATTCATCC AGATCCAGAA ATTGAAGAAA GCAAGCAAGA AACTAGTATG 
ATTTTGGATA GCGAAAAAAC AAGTGAGACT GCTGCCAAAG GGGTCAACAC AGGAGGCAGG 
GAACCAAATA CAATGGTGGA AAAAGAACGC CCTCTGGCAG ATAAGAAAGC ACAGAGACCA 
TTTGAACGAA GTGACTTTTC TGACAGCATA AAAATTCAGA CTCCAGAATT AGGTGAAGTG 
TTTCAGAATA AAGATTCTGA TTATCTGAAG AACGACAACC CTGAGGAACA TCTGAAGACC 
TCAGGGCTTG CAGGGGAGCC TGAGGGAGAA CTCTCAAAAG AGGACCATGA GAACACAGAG 
AAGTACATGG GCACAGAAAG CCAGGGGTCT GCTGCTGCAG AACCTGAAGA TGACTCGTTC 
CACTGGACTC CACATACAAG TGTAGAGCCA GGGCATAGTG ACAAGAGGGA GGACTTACTT 
ATCATAAGCA GCTTCTTTAA AGAACAACAG TCTTTGCAGC GGTTCCAGAA GTACTTTAAT 
GTCCATGAGC TGGAAGCCTT GCTACAAGAA ATGTCATCAA AACTGAAGTC AGCGCAGCAG 
GAGAGCCTGC CCTATAATAT GGAAAAAGTC CTAGATAAGG TCTTCCGTGC TTCTGAGTCA 
CAAATTCTGA GCATAGCAGA AAAAATGCTT GATACTCGTG TGGCTGAAAA TAGAGATCTG 
GGAATGAACG AAAATAACAT ATTTGAAGAG GCTGCAGTGC TTGATGACAT TCAAGACCTC 
ATCTATTTTG TCAGGTACAA GCACTCCACA GCAGAGGAGA CAGCCACACT GGTGATGGCA 
CCACCTCTAG AGGAAGGCTT GGGTGGAGCA ATGGAAGAGA TGCAACCACT GCATGAAGAT 
AATTTCTCAC GAGAGAAGAC AGCAGAACTT AATGTGCAGG TTCCTGAAGA ACCCACCCAC 
TTGGACCAAC GTGTGATTGG GGACACTCAT GCCTCAGAAG TGTCACAGAA GCCAAATACT 
GAGAAAGACC TGGACCCAGG GCCAGTTACA ACAGAAGACA CTCCTATGGA TGCTATTGAT 
GCAAACAAGC AACCAGAGAC AGCCGCCGAA GAGCCGGCAA GTGTCACACC TTTGGAAAAC 
GCAATCCTTC TAATATATTC ATTCATGTTT TATTTAACTA AGTCGCTAGT TGCTACATTG 
CCTGATGATG TTCAGCCTGG GCCTGATTTT TATGGACTGC CATGGAAACC TGTATTTATC 
ACTGCCTTCT TGGGAATTGC TTCGTTTGCC ATTTTCTTAT GGAGAACTGT CCTTGTTGTG 
AAGGATAGAG TATATCAAGT CACGGAACAG CAAATTTCTG AGAAGTTGAA GACTATCATG 
AAAGAAAATA CAGAACTTGT ACAAAAATTG TCAAATTATG AACAGAAGAT CAAGGAATCA 
AAGAAACATG TTCAGGAAAC CAGGAAACAA AATATGATTC TCTCTGATGA AGCAATTAAA 
TATAAGGATA AAATCAAGAC ACTTGAAAAA AATCAGGAAA TTCTGGATGA CACAGCTAAA 
AATCTTCGTG TTATGCTAGA ATCTGAGAGA GAACAGAATG TCAAGAATCA GGACTTGATA 
TCAGAAAACA AGAAATCTAT AGAGAAGTTA AAGGATGTTA TTTCAATGAA TGCCTCAGAA 
TTTTCAGAGG TTCAGATTGC ACTTAATGAA GCTAAGCTTA GTGAAGAGAA GGTGAAGTCT 
GAATGCCATC GGGTTCAAGA AGAAAATGCT AGGCTTAAGA AGAAAAAAGA GCAGTTGCAG 
CAGGAAATCG AAGACTGGAG TAAATTACAT GCTGAGCTCA GTGAGCAAAT CAAATCATTT 
GAGAAGTCTC AGAAAGATTT GGAAGTAGCT CTTACTCACA AGGATGATAA TATTAATGCT 
TTGACTAACT GCATTACACA GTTGAATCTG TTAGAGTGTG AATCTGAATC TGAGGGTCAA 
AATAAAGGTG GAAATGATTC AGATGAATTA GCAAATGGAG AAGTGGGAGG TGACCGGAAT 
GAGAAGATGA AAAATCAAAT TAAGCAGATG ATGGATGTCT CTCGGACACA GACTGCAATA 
TCGGTAGTTG AAGAGGATCT AAAGCTTTTA CAGCTTAAGC TAAGAGCCTC CGTGTCCACT 
AAATGTAACC TGGAAGACCA GGTAAAGAAA TTGGAAGATG ACCGCAACTC ACTACAAGCT 
GCCAAAGCTG GACTGGAAGA TGAATGCAAA ACCTTGAGGC AGAAAGTGGA GATTCTGAAT 
GAGCTCTATC AGCAGAAGGA GATGGCTTTG CAAAAGAAAC TGAGTCAAGA AGAGTATGAA 
CGGCAAGAAA GAGAGCACAG GCTGTCAGCT GCAGATGAAA AGGCAGTTTC GGCTGCAGAG 
GAAGTAAAAA CTTACAAGCG GAGAATTGAA GAAATGGAGG ATGAATTACA GAAGACAGAG 
CGGTCATTTA AAAACCAGAT CGCTACCCAT GAGAAGAAAG CTCATGAAAA CTGGCTCAAA 
GCTCGTGCTG CAGAAAGAGC TATAGCTGAA GAGAAAAGGG AAGCTGCCAA TTTGAGACAC 
AAATTATTAG AATTAACACA AAAGATGGCA ATGCTGCAAG AAGAACCTGT GATTGTAAAA 
CCAATGCCAG GAAAACCAAA TACACAAAAC CCTCCACGGA GAGGTCCTCT GAGCCAGAAT 
GGCTCTTTTG GCCCATCCCC TGTGAGTGGT GGAGAATGCT CCCCTCCATT GACAGTGGAG 
CCACCCGTGA GACCTCTCTC TGCTACTCTC AATCGAAGAG ATATGCCTAG AAGTGAATTT 
GGATCAGTGG ACGGGCCTCT ACCTCATCCT CGATGGTCAG CTGAGGCATC TGGGAAACCC 
TCTCCTTCTG ATCCAGGATC TGGTACAGCT ACCATGATGA ACAGCAGCTC AAGAGGCTCT 
TCCCCTACCA GGGTACTCGA TGAAGGCAAG CAAACTGTTC TCCAAGAGCC TGAAGTCCCC 
TCAGTTCCCA GCATTACATC TTTGGCTGAG CGTCCAGTAG CAGTTAATAT GGCTCCAAAA 
GGGCCCCCTC CTTTCCCAGG AGTCCCTCTC ATGAGCACCC CCATGGGAGG CCCTGTACCA 
CCACCCATTC GATATGGACC ACCACCTCAG CTCTGCGGAC CTTTTGGGCC TCGGCCACTT 
CCTCCACCCT TTGGCCCTGG TATGCGTCCA CCACTAGGCT TAAGAGAATT TGCACCAGGC 
GTTCCACCAG GAAGACGGGA CCTGCCTCTC CACCCTCGGG GATTTTTACC TGGACACGCA 
CCATTTAGAC CTTTAGGTTC ACTTGGCCCA AGAGAGTACT TTATTCCTGG TACCCGATTA 
CCACCCCCAA CCCATGGTCC CCAGGAATAC CCACCACCAC CTGCTGTAAG AGACTTACTG 
CCGTCAGGCT CTAGAGATGA GCCTCCACCT GCCTCTCAGA GCACTAGCCA GGACTGTTCA 
CAGGCTTTAA AACAGAGCCC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856732.1
CDS	327..5630
Translation	MEKNPELSKEREPEPEPVEANSEESDSVFSENTEDLQEQFTTQKHHSHANSQANHAQGEQASFESFEEMLQDKLKVPESENNKTSNSSQVSNEQDKIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDTEYYAVGKEDEENQEDFDELPLLTFTDGEDMKTPAKSGVEKYPTDKEQNSNEEDKVQLTVPPGIKNDDKNILTTWGDTIFSIVTGGEETRDTMDLESSSSEEEKEDDDDALVPDSKQGKPQSATDYSDPDNVDDGLFIVDIPKTNNDKEVNAEHHIKGKGRGVQESKRGLVQDKTELEDENQEGMTVHSSVHSNNLNSMPAAEKGKDTLKSAYDDTENDLKGAAIHISKGMLHEEKPGEQILEGGSESESAQKAAGNQMNDRKIQQESLGSAPLMGDDHPNASRDSVEGDALVNGAKLHTLSVEHQREELKEELVLKTQNQPRFSSPDEIDLPRELEDEVPILGRNLPWQQERDVAATASKQMSEKIRLSEGEAKEDSLDEEFFHHKAMQGTEVGQTDQTDSTGGPAFLSKVEEDDYPSEELLEDENAINAKRSKEKNPGNQGRQFDVNLQVPDRAVLGTIHPDPEIEESKQETSMILDSEKTSETAAKGVNTGGREPNTMVEKERPLADKKAQRPFERSDFSDSIKIQTPELGEVFQNKDSDYLKNDNPEEHLKTSGLAGEPEGELSKEDHENTEKYMGTESQGSAAAEPEDDSFHWTPHTSVEPGHSDKREDLLIISSFFKEQQSLQRFQKYFNVHELEALLQEMSSKLKSAQQESLPYNMEKVLDKVFRASESQILSIAEKMLDTRVAENRDLGMNENNIFEEAAVLDDIQDLIYFVRYKHSTAEETATLVMAPPLEEGLGGAMEEMQPLHEDNFSREKTAELNVQVPEEPTHLDQRVIGDTHASEVSQKPNTEKDLDPGPVTTEDTPMDAIDANKQPETAAEEPASVTPLENAILLIYSFMFYLTKSLVATLPDDVQPGPDFYGLPWKPVFITAFLGIASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLISENKKSIEKLKDVISMNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREHRLSAADEKAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGKPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKQTVLQEPEVPSVPSITSLAERPVAVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_017001243.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001243.2

ORF Insert Sequence:

ATGGAAAAAA ACCCTGAATT ATCTAAGGAA AGGGAACCTG AACCTGAACC AGTAGAAGCC 
AACTCAGAGG AAAGTGATAG TGTATTCTCA GAAAACACTG AGGATCTTCA GGAACAGTTT 
ACAACTCAGA AGCACCACTC CCATGCAAAC AGCCAAGCAA ATCATGCTCA GGGAGAGCAG 
GCTTCATTTG AATCTTTTGA AGAAATGCTG CAAGATAAAC TAAAAGTGCC AGAAAGTGAA 
AACAACAAAA CCAGCAATAG TTCTCAGGTC TCAAATGAAC AGGATAAGAT TGATGCCTAT 
AAACTTTTGA AAAAAGAAAT GACTCTAGAC TTGAAAACCA AATTTGGCTC AACAGCTGAT 
GCACTTGTAT CTGATGATGA GACAACCAGA CTCGTTACTT CATTAGAAGA TGATTTTGAT 
GAGGAATTGG ATACTGAGTA TTATGCAGTT GGAAAGGAAG ATGAGGAGAA CCAAGAAGAC 
TTTGATGAGT TGCCATTACT TACCTTTACA GATGGGGAAG ATATGAAAAC TCCAGCAAAG 
TCTGGCGTTG AGAAATATCC AACAGATAAA GAGCAGAATT CAAATGAAGA GGACAAGGTT 
CAGCTAACTG TGCCCCCTGG CATCAAAAAT GATGATAAAA ATATACTAAC AACCTGGGGG 
GACACTATCT TCTCTATTGT CACAGGAGGT GAAGAAACAA GAGATACGAT GGATTTAGAG 
AGCTCTAGTT CAGAGGAAGA AAAAGAAGAT GATGATGATG CATTAGTCCC AGATAGCAAA 
CAGGGGAAAC CACAGTCAGC AACAGATTAT AGTGACCCTG ACAATGTAGA TGATGGTCTT 
TTTATTGTAG ACATTCCTAA AACAAATAAT GACAAAGAAG TAAACGCAGA ACATCACATT 
AAAGGAAAAG GGAGGGGAGT TCAGGAATCC AAGAGGGGCC TGGTACAAGA TAAGACAGAA 
TTAGAGGATG AAAATCAAGA AGGCATGACT GTGCACAGTT CTGTTCACAG CAATAACCTC 
AACTCTATGC CAGCTGCTGA AAAGGGTAAA GACACATTAA AATCAGCTTA TGATGATACA 
GAAAATGACC TAAAAGGAGC AGCTATTCAT ATCTCAAAAG GAATGCTCCA CGAAGAAAAG 
CCTGGAGAGC AGATTTTGGA AGGTGGCTCA GAGAGTGAAT CTGCACAGAA AGCTGCAGGG 
AATCAAATGA ATGACAGAAA GATTCAACAG GAATCCCTGG GTAGTGCACC ACTCATGGGA 
GATGACCACC CTAACGCATC CAGAGACAGT GTGGAGGGAG ACGCTTTGGT AAATGGGGCC 
AAACTGCACA CGCTTTCAGT GGAGCATCAA CGTGAGGAAT TGAAAGAGGA ATTAGTTCTT 
AAAACTCAAA ACCAACCTAG ATTCTCCTCT CCAGATGAGA TTGATTTGCC CAGAGAACTG 
GAAGACGAGG TTCCCATTCT GGGAAGAAAT CTTCCCTGGC AACAAGAAAG AGATGTGGCT 
GCCACAGCCA GTAAGCAAAT GAGTGAGAAG ATAAGGCTCT CTGAGGGAGA AGCCAAAGAG 
GACTCCTTGG ATGAAGAGTT TTTTCATCAC AAGGCAATGC AGGGCACAGA GGTAGGACAG 
ACAGACCAAA CTGACAGCAC AGGAGGACCA GCTTTCCTTT CTAAAGTAGA AGAGGATGAT 
TATCCCTCTG AAGAACTACT AGAGGATGAA AACGCTATAA ATGCAAAACG GTCTAAAGAA 
AAAAACCCTG GGAATCAGGG CAGGCAGTTT GATGTTAATC TGCAAGTCCC TGACAGAGCA 
GTTTTAGGGA CCATTCATCC AGATCCAGAA ATTGAAGAAA GCAAGCAAGA AACTAGTATG 
ATTTTGGATA GCGAAAAAAC AAGTGAGACT GCTGCCAAAG GGGTCAACAC AGGAGGCAGG 
GAACCAAATA CAATGGTGGA AAAAGAACGC CCTCTGGCAG ATAAGAAAGC ACAGAGACCA 
TTTGAACGAA GTGACTTTTC TGACAGCATA AAAATTCAGA CTCCAGAATT AGGTGAAGTG 
TTTCAGAATA AAGATTCTGA TTATCTGAAG AACGACAACC CTGAGGAACA TCTGAAGACC 
TCAGGGCTTG CAGGGGAGCC TGAGGGAGAA CTCTCAAAAG AGGACCATGA GAACACAGAG 
AAGTACATGG GCACAGAAAG CCAGGGGTCT GCTGCTGCAG AACCTGAAGA TGACTCGTTC 
CACTGGACTC CACATACAAG TGTAGAGCCA GGGCATAGTG ACAAGAGGGA GGACTTACTT 
ATCATAAGCA GCTTCTTTAA AGAACAACAG TCTTTGCAGC GGTTCCAGAA GTACTTTAAT 
GTCCATGAGC TGGAAGCCTT GCTACAAGAA ATGTCATCAA AACTGAAGTC AGCGCAGCAG 
GAGAGCCTGC CCTATAATAT GGAAAAAGTC CTAGATAAGG TCTTCCGTGC TTCTGAGTCA 
CAAATTCTGA GCATAGCAGA AAAAATGCTT GATACTCGTG TGGCTGAAAA TAGAGATCTG 
GGAATGAACG AAAATAACAT ATTTGAAGAG GCTGCAGTGC TTGATGACAT TCAAGACCTC 
ATCTATTTTG TCAGGTACAA GCACTCCACA GCAGAGGAGA CAGCCACACT GGTGATGGCA 
CCACCTCTAG AGGAAGGCTT GGGTGGAGCA ATGGAAGAGA TGCAACCACT GCATGAAGAT 
AATTTCTCAC GAGAGAAGAC AGCAGAACTT AATGTGCAGG TTCCTGAAGA ACCCACCCAC 
TTGGACCAAC GTGTGATTGG GGACACTCAT GCCTCAGAAG TGTCACAGAA GCCAAATACT 
GAGAAAGACC TGGACCCAGG GCCAGTTACA ACAGAAGACA CTCCTATGGA TGCTATTGAT 
GCAAACAAGC AACCAGAGAC AGCCGCCGAA GAGCCGGCAA GTGTCACACC TTTGGAAAAC 
GCAATCCTTC TAATATATTC ATTCATGTTT TATTTAACTA AGTCGCTAGT TGCTACATTG 
CCTGATGATG TTCAGCCTGG GCCTGATTTT TATGGACTGC CATGGAAACC TGTATTTATC 
ACTGCCTTCT TGGGAATTGC TTCGTTTGCC ATTTTCTTAT GGAGAACTGT CCTTGTTGTG 
AAGGATAGAG TATATCAAGT CACGGAACAG CAAATTTCTG AGAAGTTGAA GACTATCATG 
AAAGAAAATA CAGAACTTGT ACAAAAATTG TCAAATTATG AACAGAAGAT CAAGGAATCA 
AAGAAACATG TTCAGGAAAC CAGGAAACAA AATATGATTC TCTCTGATGA AGCAATTAAA 
TATAAGGATA AAATCAAGAC ACTTGAAAAA AATCAGGAAA TTCTGGATGA CACAGCTAAA 
AATCTTCGTG TTATGCTAGA ATCTGAGAGA GAACAGAATG TCAAGAATCA GGACTTGATA 
TCAGAAAACA AGAAATCTAT AGAGAAGTTA AAGGATGTTA TTTCAATGAA TGCCTCAGAA 
TTTTCAGAGG TTCAGATTGC ACTTAATGAA GCTAAGCTTA GTGAAGAGAA GGTGAAGTCT 
GAATGCCATC GGGTTCAAGA AGAAAATGCT AGGCTTAAGA AGAAAAAAGA GCAGTTGCAG 
CAGGAAATCG AAGACTGGAG TAAATTACAT GCTGAGCTCA GTGAGCAAAT CAAATCATTT 
GAGAAGTCTC AGAAAGATTT GGAAGTAGCT CTTACTCACA AGGATGATAA TATTAATGCT 
TTGACTAACT GCATTACACA GTTGAATCTG TTAGAGTGTG AATCTGAATC TGAGGGTCAA 
AATAAAGGTG GAAATGATTC AGATGAATTA GCAAATGGAG AAGTGGGAGG TGACCGGAAT 
GAGAAGATGA AAAATCAAAT TAAGCAGATG ATGGATGTCT CTCGGACACA GACTGCAATA 
TCGGTAGTTG AAGAGGATCT AAAGCTTTTA CAGCTTAAGC TAAGAGCCTC CGTGTCCACT 
AAATGTAACC TGGAAGACCA GGTAAAGAAA TTGGAAGATG ACCGCAACTC ACTACAAGCT 
GCCAAAGCTG GACTGGAAGA TGAATGCAAA ACCTTGAGGC AGAAAGTGGA GATTCTGAAT 
GAGCTCTATC AGCAGAAGGA GATGGCTTTG CAAAAGAAAC TGAGTCAAGA AGAGTATGAA 
CGGCAAGAAA GAGAGCACAG GCTGTCAGCT GCAGATGAAA AGGCAGTTTC GGCTGCAGAG 
GAAGTAAAAA CTTACAAGCG GAGAATTGAA GAAATGGAGG ATGAATTACA GAAGACAGAG 
CGGTCATTTA AAAACCAGAT CGCTACCCAT GAGAAGAAAG CTCATGAAAA CTGGCTCAAA 
GCTCGTGCTG CAGAAAGAGC TATAGCTGAA GAGAAAAGGG AAGCTGCCAA TTTGAGACAC 
AAATTATTAG AATTAACACA AAAGATGGCA ATGCTGCAAG AAGAACCTGT GATTGTAAAA 
CCAATGCCAG GAAAACCAAA TACACAAAAC CCTCCACGGA GAGGTCCTCT GAGCCAGAAT 
GGCTCTTTTG GCCCATCCCC TGTGAGTGGT GGAGAATGCT CCCCTCCATT GACAGTGGAG 
CCACCCGTGA GACCTCTCTC TGCTACTCTC AATCGAAGAG ATATGCCTAG AAGTGAATTT 
GGATCAGTGG ACGGGCCTCT ACCTCATCCT CGATGGTCAG CTGAGGCATC TGGGAAACCC 
TCTCCTTCTG ATCCAGGATC TGGTACAGCT ACCATGATGA ACAGCAGCTC AAGAGGCTCT 
TCCCCTACCA GGGTACTCGA TGAAGGCAAG CAAACTGTTC TCCAAGAGCC TGAAGTCCCC 
TCAGTTCCCA GCATTACATC TTTGGCTGAG CGTCCAGTAG CAGTTAATAT GGCTCCAAAA 
GGGCCCCCTC CTTTCCCAGG AGTCCCTCTC ATGAGCACCC CCATGGGAGG CCCTGTACCA 
CCACCCATTC GATATGGACC ACCACCTCAG CTCTGCGGAC CTTTTGGGCC TCGGCCACTT 
CCTCCACCCT TTGGCCCTGG TATGCGTCCA CCACTAGGCT TAAGAGAATT TGCACCAGGC 
GTTCCACCAG GAAGACGGGA CCTGCCTCTC CACCCTCGGG GATTTTTACC TGGACACGCA 
CCATTTAGAC CTTTAGGTTC ACTTGGCCCA AGAGAGTACT TTATTCCTGG TACCCGATTA 
CCACCCCCAA CCCATGGTCC CCAGGAATAC CCACCACCAC CTGCTGTAAG AGACTTACTG 
CCGTCAGGCT CTAGAGATGA GCCTCCACCT GCCTCTCAGA GCACTAGCCA GGACTGTTCA 
CAGGCTTTAA AACAGAGCCC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( NM_198551.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_940953.2 Homo sapiens MIA family member 3, ER export factor (MIA3), transcript variant 1, mRNA.

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CloneID	OHu13395
Clone ID Related Accession (Same CDS sequence)	NM_198551.3 , NM_198551.4
Accession Version	NM_198551.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	transport and Golgi organization protein 1 homolog isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA040423.1, AL592148.7, AK096526.1, D87742.1, BP423646.1 and CK724924.1. On Jul 15, 2014 this sequence version replaced NM_198551.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGG CGCCTGGGCT GCTCGTCTGG CTGCTCGTGC TCCGGCTGCC CTGGCGGGTG 
CCGGGCCAGC TGGACCCCAG CACTGGCCGG CGGTTCTCGG AGCACAAACT CTGCGCGGAC 
GACGAATGCA GCATGTTAAT GTACCGCGGT GAGGCTCTTG AAGATTTCAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGTGAT CCTGTATATG TTTACTATAA ACTGGCAAGA 
GGATGGCCTG AAGTTTGGGC TGGAAGTGTT GGACGCACTT TTGGATATTT TCCAAAAGAT 
TTAATCCAGG TAGTTCATGA ATATACCAAA GAAGAGCTAC AAGTTCCAAC AGATGAGACG 
GATTTTGTTT GTTTTGATGG AGGAAGAGAT GATTTTCATA ATTATAATGT AGAAGAACTT 
TTAGGGTTTT TGGAACTGTA CAATTCTGCA GCTACAGATT CTGAGAAAGC TGTAGAAAAA 
ACTTTACAGG ATATGGAAAA AAACCCTGAA TTATCTAAGG AAAGGGAACC TGAACCTGAA 
CCAGTAGAAG CCAACTCAGA GGAAAGTGAT AGTGTATTCT CAGAAAACAC TGAGGATCTT 
CAGGAACAGT TTACAACTCA GAAGCACCAC TCCCATGCAA ACAGCCAAGC AAATCATGCT 
CAGGGAGAGC AGGCTTCATT TGAATCTTTT GAAGAAATGC TGCAAGATAA ACTAAAAGTG 
CCAGAAAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCAAATGA ACAGGATAAG 
ATTGATGCCT ATAAACTTTT GAAAAAAGAA ATGACTCTAG ACTTGAAAAC CAAATTTGGC 
TCAACAGCTG ATGCACTTGT ATCTGATGAT GAGACAACCA GACTCGTTAC TTCATTAGAA 
GATGATTTTG ATGAGGAATT GGATACTGAG TATTATGCAG TTGGAAAGGA AGATGAGGAG 
AACCAAGAAG ACTTTGATGA GTTGCCATTA CTTACCTTTA CAGATGGGGA AGATATGAAA 
ACTCCAGCAA AGTCTGGCGT TGAGAAATAT CCAACAGATA AAGAGCAGAA TTCAAATGAA 
GAGGACAAGG TTCAGCTAAC TGTGCCCCCT GGCATCAAAA ATGATGATAA AAATATACTA 
ACAACCTGGG GGGACACTAT CTTCTCTATT GTCACAGGAG GTGAAGAAAC AAGAGATACG 
ATGGATTTAG AGAGCTCTAG TTCAGAGGAA GAAAAAGAAG ATGATGATGA TGCATTAGTC 
CCAGATAGCA AACAGGGGAA ACCACAGTCA GCAACAGATT ATAGTGACCC TGACAATGTA 
GATGATGGTC TTTTTATTGT AGACATTCCT AAAACAAATA ATGACAAAGA AGTAAACGCA 
GAACATCACA TTAAAGGAAA AGGGAGGGGA GTTCAGGAAT CCAAGAGGGG CCTGGTACAA 
GATAAGACAG AATTAGAGGA TGAAAATCAA GAAGGCATGA CTGTGCACAG TTCTGTTCAC 
AGCAATAACC TCAACTCTAT GCCAGCTGCT GAAAAGGGTA AAGACACATT AAAATCAGCT 
TATGATGATA CAGAAAATGA CCTAAAAGGA GCAGCTATTC ATATCTCAAA AGGAATGCTC 
CACGAAGAAA AGCCTGGAGA GCAGATTTTG GAAGGTGGCT CAGAGAGTGA ATCTGCACAG 
AAAGCTGCAG GGAATCAAAT GAATGACAGA AAGATTCAAC AGGAATCCCT GGGTAGTGCA 
CCACTCATGG GAGATGACCA CCCTAACGCA TCCAGAGACA GTGTGGAGGG AGACGCTTTG 
GTAAATGGGG CCAAACTGCA CACGCTTTCA GTGGAGCATC AACGTGAGGA ATTGAAAGAG 
GAATTAGTTC TTAAAACTCA AAACCAACCT AGATTCTCCT CTCCAGATGA GATTGATTTG 
CCCAGAGAAC TGGAAGACGA GGTTCCCATT CTGGGAAGAA ATCTTCCCTG GCAACAAGAA 
AGAGATGTGG CTGCCACAGC CAGTAAGCAA ATGAGTGAGA AGATAAGGCT CTCTGAGGGA 
GAAGCCAAAG AGGACTCCTT GGATGAAGAG TTTTTTCATC ACAAGGCAAT GCAGGGCACA 
GAGGTAGGAC AGACAGACCA AACTGACAGC ACAGGAGGAC CAGCTTTCCT TTCTAAAGTA 
GAAGAGGATG ATTATCCCTC TGAAGAACTA CTAGAGGATG AAAACGCTAT AAATGCAAAA 
CGGTCTAAAG AAAAAAACCC TGGGAATCAG GGCAGGCAGT TTGATGTTAA TCTGCAAGTC 
CCTGACAGAG CAGTTTTAGG GACCATTCAT CCAGATCCAG AAATTGAAGA AAGCAAGCAA 
GAAACTAGTA TGATTTTGGA TAGCGAAAAA ACAAGTGAGA CTGCTGCCAA AGGGGTCAAC 
ACAGGAGGCA GGGAACCAAA TACAATGGTG GAAAAAGAAC GCCCTCTGGC AGATAAGAAA 
GCACAGAGAC CATTTGAACG AAGTGACTTT TCTGACAGCA TAAAAATTCA GACTCCAGAA 
TTAGGTGAAG TGTTTCAGAA TAAAGATTCT GATTATCTGA AGAACGACAA CCCTGAGGAA 
CATCTGAAGA CCTCAGGGCT TGCAGGGGAG CCTGAGGGAG AACTCTCAAA AGAGGACCAT 
GAGAACACAG AGAAGTACAT GGGCACAGAA AGCCAGGGGT CTGCTGCTGC AGAACCTGAA 
GATGACTCGT TCCACTGGAC TCCACATACA AGTGTAGAGC CAGGGCATAG TGACAAGAGG 
GAGGACTTAC TTATCATAAG CAGCTTCTTT AAAGAACAAC AGTCTTTGCA GCGGTTCCAG 
AAGTACTTTA ATGTCCATGA GCTGGAAGCC TTGCTACAAG AAATGTCATC AAAACTGAAG 
TCAGCGCAGC AGGAGAGCCT GCCCTATAAT ATGGAAAAAG TCCTAGATAA GGTCTTCCGT 
GCTTCTGAGT CACAAATTCT GAGCATAGCA GAAAAAATGC TTGATACTCG TGTGGCTGAA 
AATAGAGATC TGGGAATGAA CGAAAATAAC ATATTTGAAG AGGCTGCAGT GCTTGATGAC 
ATTCAAGACC TCATCTATTT TGTCAGGTAC AAGCACTCCA CAGCAGAGGA GACAGCCACA 
CTGGTGATGG CACCACCTCT AGAGGAAGGC TTGGGTGGAG CAATGGAAGA GATGCAACCA 
CTGCATGAAG ATAATTTCTC ACGAGAGAAG ACAGCAGAAC TTAATGTGCA GGTTCCTGAA 
GAACCCACCC ACTTGGACCA ACGTGTGATT GGGGACACTC ATGCCTCAGA AGTGTCACAG 
AAGCCAAATA CTGAGAAAGA CCTGGACCCA GGGCCAGTTA CAACAGAAGA CACTCCTATG 
GATGCTATTG ATGCAAACAA GCAACCAGAG ACAGCCGCCG AAGAGCCGGC AAGTGTCACA 
CCTTTGGAAA ACGCAATCCT TCTAATATAT TCATTCATGT TTTATTTAAC TAAGTCGCTA 
GTTGCTACAT TGCCTGATGA TGTTCAGCCT GGGCCTGATT TTTATGGACT GCCATGGAAA 
CCTGTATTTA TCACTGCCTT CTTGGGAATT GCTTCGTTTG CCATTTTCTT ATGGAGAACT 
GTCCTTGTTG TGAAGGATAG AGTATATCAA GTCACGGAAC AGCAAATTTC TGAGAAGTTG 
AAGACTATCA TGAAAGAAAA TACAGAACTT GTACAAAAAT TGTCAAATTA TGAACAGAAG 
ATCAAGGAAT CAAAGAAACA TGTTCAGGAA ACCAGGAAAC AAAATATGAT TCTCTCTGAT 
GAAGCAATTA AATATAAGGA TAAAATCAAG ACACTTGAAA AAAATCAGGA AATTCTGGAT 
GACACAGCTA AAAATCTTCG TGTTATGCTA GAATCTGAGA GAGAACAGAA TGTCAAGAAT 
CAGGACTTGA TATCAGAAAA CAAGAAATCT ATAGAGAAGT TAAAGGATGT TATTTCAATG 
AATGCCTCAG AATTTTCAGA GGTTCAGATT GCACTTAATG AAGCTAAGCT TAGTGAAGAG 
AAGGTGAAGT CTGAATGCCA TCGGGTTCAA GAAGAAAATG CTAGGCTTAA GAAGAAAAAA 
GAGCAGTTGC AGCAGGAAAT CGAAGACTGG AGTAAATTAC ATGCTGAGCT CAGTGAGCAA 
ATCAAATCAT TTGAGAAGTC TCAGAAAGAT TTGGAAGTAG CTCTTACTCA CAAGGATGAT 
AATATTAATG CTTTGACTAA CTGCATTACA CAGTTGAATC TGTTAGAGTG TGAATCTGAA 
TCTGAGGGTC AAAATAAAGG TGGAAATGAT TCAGATGAAT TAGCAAATGG AGAAGTGGGA 
GGTGACCGGA ATGAGAAGAT GAAAAATCAA ATTAAGCAGA TGATGGATGT CTCTCGGACA 
CAGACTGCAA TATCGGTAGT TGAAGAGGAT CTAAAGCTTT TACAGCTTAA GCTAAGAGCC 
TCCGTGTCCA CTAAATGTAA CCTGGAAGAC CAGGTAAAGA AATTGGAAGA TGACCGCAAC 
TCACTACAAG CTGCCAAAGC TGGACTGGAA GATGAATGCA AAACCTTGAG GCAGAAAGTG 
GAGATTCTGA ATGAGCTCTA TCAGCAGAAG GAGATGGCTT TGCAAAAGAA ACTGAGTCAA 
GAAGAGTATG AACGGCAAGA AAGAGAGCAC AGGCTGTCAG CTGCAGATGA AAAGGCAGTT 
TCGGCTGCAG AGGAAGTAAA AACTTACAAG CGGAGAATTG AAGAAATGGA GGATGAATTA 
CAGAAGACAG AGCGGTCATT TAAAAACCAG ATCGCTACCC ATGAGAAGAA AGCTCATGAA 
AACTGGCTCA AAGCTCGTGC TGCAGAAAGA GCTATAGCTG AAGAGAAAAG GGAAGCTGCC 
AATTTGAGAC ACAAATTATT AGAATTAACA CAAAAGATGG CAATGCTGCA AGAAGAACCT 
GTGATTGTAA AACCAATGCC AGGAAAACCA AATACACAAA ACCCTCCACG GAGAGGTCCT 
CTGAGCCAGA ATGGCTCTTT TGGCCCATCC CCTGTGAGTG GTGGAGAATG CTCCCCTCCA 
TTGACAGTGG AGCCACCCGT GAGACCTCTC TCTGCTACTC TCAATCGAAG AGATATGCCT 
AGAAGTGAAT TTGGATCAGT GGACGGGCCT CTACCTCATC CTCGATGGTC AGCTGAGGCA 
TCTGGGAAAC CCTCTCCTTC TGATCCAGGA TCTGGTACAG CTACCATGAT GAACAGCAGC 
TCAAGAGGCT CTTCCCCTAC CAGGGTACTC GATGAAGGCA AGGTTAATAT GGCTCCAAAA 
GGGCCCCCTC CTTTCCCAGG AGTCCCTCTC ATGAGCACCC CCATGGGAGG CCCTGTACCA 
CCACCCATTC GATATGGACC ACCACCTCAG CTCTGCGGAC CTTTTGGGCC TCGGCCACTT 
CCTCCACCCT TTGGCCCTGG TATGCGTCCA CCACTAGGCT TAAGAGAATT TGCACCAGGC 
GTTCCACCAG GAAGACGGGA CCTGCCTCTC CACCCTCGGG GATTTTTACC TGGACACGCA 
CCATTTAGAC CTTTAGGTTC ACTTGGCCCA AGAGAGTACT TTATTCCTGG TACCCGATTA 
CCACCCCCAA CCCATGGTCC CCAGGAATAC CCACCACCAC CTGCTGTAAG AGACTTACTG 
CCGTCAGGCT CTAGAGATGA GCCTCCACCT GCCTCTCAGA GCACTAGCCA GGACTGTTCA 
CAGGCTTTAA AACAGAGCCC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_940953.2
CDS	10..5733
Translation	MAAAPGLLVWLLVLRLPWRVPGQLDPSTGRRFSEHKLCADDECSMLMYRGEALEDFTGPDCRFVNFKKGDPVYVYYKLARGWPEVWAGSVGRTFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFHNYNVEELLGFLELYNSAATDSEKAVEKTLQDMEKNPELSKEREPEPEPVEANSEESDSVFSENTEDLQEQFTTQKHHSHANSQANHAQGEQASFESFEEMLQDKLKVPESENNKTSNSSQVSNEQDKIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDTEYYAVGKEDEENQEDFDELPLLTFTDGEDMKTPAKSGVEKYPTDKEQNSNEEDKVQLTVPPGIKNDDKNILTTWGDTIFSIVTGGEETRDTMDLESSSSEEEKEDDDDALVPDSKQGKPQSATDYSDPDNVDDGLFIVDIPKTNNDKEVNAEHHIKGKGRGVQESKRGLVQDKTELEDENQEGMTVHSSVHSNNLNSMPAAEKGKDTLKSAYDDTENDLKGAAIHISKGMLHEEKPGEQILEGGSESESAQKAAGNQMNDRKIQQESLGSAPLMGDDHPNASRDSVEGDALVNGAKLHTLSVEHQREELKEELVLKTQNQPRFSSPDEIDLPRELEDEVPILGRNLPWQQERDVAATASKQMSEKIRLSEGEAKEDSLDEEFFHHKAMQGTEVGQTDQTDSTGGPAFLSKVEEDDYPSEELLEDENAINAKRSKEKNPGNQGRQFDVNLQVPDRAVLGTIHPDPEIEESKQETSMILDSEKTSETAAKGVNTGGREPNTMVEKERPLADKKAQRPFERSDFSDSIKIQTPELGEVFQNKDSDYLKNDNPEEHLKTSGLAGEPEGELSKEDHENTEKYMGTESQGSAAAEPEDDSFHWTPHTSVEPGHSDKREDLLIISSFFKEQQSLQRFQKYFNVHELEALLQEMSSKLKSAQQESLPYNMEKVLDKVFRASESQILSIAEKMLDTRVAENRDLGMNENNIFEEAAVLDDIQDLIYFVRYKHSTAEETATLVMAPPLEEGLGGAMEEMQPLHEDNFSREKTAELNVQVPEEPTHLDQRVIGDTHASEVSQKPNTEKDLDPGPVTTEDTPMDAIDANKQPETAAEEPASVTPLENAILLIYSFMFYLTKSLVATLPDDVQPGPDFYGLPWKPVFITAFLGIASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLISENKKSIEKLKDVISMNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREHRLSAADEKAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGKPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

Target ORF information:

RefSeq Version	NM_198551.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MIA family member 3, ER export factor (MIA3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198551.3

ORF Insert Sequence:

ATGGCTGCGG CGCCTGGGCT GCTCGTCTGG CTGCTCGTGC TCCGGCTGCC CTGGCGGGTG 
CCGGGCCAGC TGGACCCCAG CACTGGCCGG CGGTTCTCGG AGCACAAACT CTGCGCGGAC 
GACGAATGCA GCATGTTAAT GTACCGCGGT GAGGCTCTTG AAGATTTCAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGTGAT CCTGTATATG TTTACTATAA ACTGGCAAGA 
GGATGGCCTG AAGTTTGGGC TGGAAGTGTT GGACGCACTT TTGGATATTT TCCAAAAGAT 
TTAATCCAGG TAGTTCATGA ATATACCAAA GAAGAGCTAC AAGTTCCAAC AGATGAGACG 
GATTTTGTTT GTTTTGATGG AGGAAGAGAT GATTTTCATA ATTATAATGT AGAAGAACTT 
TTAGGGTTTT TGGAACTGTA CAATTCTGCA GCTACAGATT CTGAGAAAGC TGTAGAAAAA 
ACTTTACAGG ATATGGAAAA AAACCCTGAA TTATCTAAGG AAAGGGAACC TGAACCTGAA 
CCAGTAGAAG CCAACTCAGA GGAAAGTGAT AGTGTATTCT CAGAAAACAC TGAGGATCTT 
CAGGAACAGT TTACAACTCA GAAGCACCAC TCCCATGCAA ACAGCCAAGC AAATCATGCT 
CAGGGAGAGC AGGCTTCATT TGAATCTTTT GAAGAAATGC TGCAAGATAA ACTAAAAGTG 
CCAGAAAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCAAATGA ACAGGATAAG 
ATTGATGCCT ATAAACTTTT GAAAAAAGAA ATGACTCTAG ACTTGAAAAC CAAATTTGGC 
TCAACAGCTG ATGCACTTGT ATCTGATGAT GAGACAACCA GACTCGTTAC TTCATTAGAA 
GATGATTTTG ATGAGGAATT GGATACTGAG TATTATGCAG TTGGAAAGGA AGATGAGGAG 
AACCAAGAAG ACTTTGATGA GTTGCCATTA CTTACCTTTA CAGATGGGGA AGATATGAAA 
ACTCCAGCAA AGTCTGGCGT TGAGAAATAT CCAACAGATA AAGAGCAGAA TTCAAATGAA 
GAGGACAAGG TTCAGCTAAC TGTGCCCCCT GGCATCAAAA ATGATGATAA AAATATACTA 
ACAACCTGGG GGGACACTAT CTTCTCTATT GTCACAGGAG GTGAAGAAAC AAGAGATACG 
ATGGATTTAG AGAGCTCTAG TTCAGAGGAA GAAAAAGAAG ATGATGATGA TGCATTAGTC 
CCAGATAGCA AACAGGGGAA ACCACAGTCA GCAACAGATT ATAGTGACCC TGACAATGTA 
GATGATGGTC TTTTTATTGT AGACATTCCT AAAACAAATA ATGACAAAGA AGTAAACGCA 
GAACATCACA TTAAAGGAAA AGGGAGGGGA GTTCAGGAAT CCAAGAGGGG CCTGGTACAA 
GATAAGACAG AATTAGAGGA TGAAAATCAA GAAGGCATGA CTGTGCACAG TTCTGTTCAC 
AGCAATAACC TCAACTCTAT GCCAGCTGCT GAAAAGGGTA AAGACACATT AAAATCAGCT 
TATGATGATA CAGAAAATGA CCTAAAAGGA GCAGCTATTC ATATCTCAAA AGGAATGCTC 
CACGAAGAAA AGCCTGGAGA GCAGATTTTG GAAGGTGGCT CAGAGAGTGA ATCTGCACAG 
AAAGCTGCAG GGAATCAAAT GAATGACAGA AAGATTCAAC AGGAATCCCT GGGTAGTGCA 
CCACTCATGG GAGATGACCA CCCTAACGCA TCCAGAGACA GTGTGGAGGG AGACGCTTTG 
GTAAATGGGG CCAAACTGCA CACGCTTTCA GTGGAGCATC AACGTGAGGA ATTGAAAGAG 
GAATTAGTTC TTAAAACTCA AAACCAACCT AGATTCTCCT CTCCAGATGA GATTGATTTG 
CCCAGAGAAC TGGAAGACGA GGTTCCCATT CTGGGAAGAA ATCTTCCCTG GCAACAAGAA 
AGAGATGTGG CTGCCACAGC CAGTAAGCAA ATGAGTGAGA AGATAAGGCT CTCTGAGGGA 
GAAGCCAAAG AGGACTCCTT GGATGAAGAG TTTTTTCATC ACAAGGCAAT GCAGGGCACA 
GAGGTAGGAC AGACAGACCA AACTGACAGC ACAGGAGGAC CAGCTTTCCT TTCTAAAGTA 
GAAGAGGATG ATTATCCCTC TGAAGAACTA CTAGAGGATG AAAACGCTAT AAATGCAAAA 
CGGTCTAAAG AAAAAAACCC TGGGAATCAG GGCAGGCAGT TTGATGTTAA TCTGCAAGTC 
CCTGACAGAG CAGTTTTAGG GACCATTCAT CCAGATCCAG AAATTGAAGA AAGCAAGCAA 
GAAACTAGTA TGATTTTGGA TAGCGAAAAA ACAAGTGAGA CTGCTGCCAA AGGGGTCAAC 
ACAGGAGGCA GGGAACCAAA TACAATGGTG GAAAAAGAAC GCCCTCTGGC AGATAAGAAA 
GCACAGAGAC CATTTGAACG AAGTGACTTT TCTGACAGCA TAAAAATTCA GACTCCAGAA 
TTAGGTGAAG TGTTTCAGAA TAAAGATTCT GATTATCTGA AGAACGACAA CCCTGAGGAA 
CATCTGAAGA CCTCAGGGCT TGCAGGGGAG CCTGAGGGAG AACTCTCAAA AGAGGACCAT 
GAGAACACAG AGAAGTACAT GGGCACAGAA AGCCAGGGGT CTGCTGCTGC AGAACCTGAA 
GATGACTCGT TCCACTGGAC TCCACATACA AGTGTAGAGC CAGGGCATAG TGACAAGAGG 
GAGGACTTAC TTATCATAAG CAGCTTCTTT AAAGAACAAC AGTCTTTGCA GCGGTTCCAG 
AAGTACTTTA ATGTCCATGA GCTGGAAGCC TTGCTACAAG AAATGTCATC AAAACTGAAG 
TCAGCGCAGC AGGAGAGCCT GCCCTATAAT ATGGAAAAAG TCCTAGATAA GGTCTTCCGT 
GCTTCTGAGT CACAAATTCT GAGCATAGCA GAAAAAATGC TTGATACTCG TGTGGCTGAA 
AATAGAGATC TGGGAATGAA CGAAAATAAC ATATTTGAAG AGGCTGCAGT GCTTGATGAC 
ATTCAAGACC TCATCTATTT TGTCAGGTAC AAGCACTCCA CAGCAGAGGA GACAGCCACA 
CTGGTGATGG CACCACCTCT AGAGGAAGGC TTGGGTGGAG CAATGGAAGA GATGCAACCA 
CTGCATGAAG ATAATTTCTC ACGAGAGAAG ACAGCAGAAC TTAATGTGCA GGTTCCTGAA 
GAACCCACCC ACTTGGACCA ACGTGTGATT GGGGACACTC ATGCCTCAGA AGTGTCACAG 
AAGCCAAATA CTGAGAAAGA CCTGGACCCA GGGCCAGTTA CAACAGAAGA CACTCCTATG 
GATGCTATTG ATGCAAACAA GCAACCAGAG ACAGCCGCCG AAGAGCCGGC AAGTGTCACA 
CCTTTGGAAA ACGCAATCCT TCTAATATAT TCATTCATGT TTTATTTAAC TAAGTCGCTA 
GTTGCTACAT TGCCTGATGA TGTTCAGCCT GGGCCTGATT TTTATGGACT GCCATGGAAA 
CCTGTATTTA TCACTGCCTT CTTGGGAATT GCTTCGTTTG CCATTTTCTT ATGGAGAACT 
GTCCTTGTTG TGAAGGATAG AGTATATCAA GTCACGGAAC AGCAAATTTC TGAGAAGTTG 
AAGACTATCA TGAAAGAAAA TACAGAACTT GTACAAAAAT TGTCAAATTA TGAACAGAAG 
ATCAAGGAAT CAAAGAAACA TGTTCAGGAA ACCAGGAAAC AAAATATGAT TCTCTCTGAT 
GAAGCAATTA AATATAAGGA TAAAATCAAG ACACTTGAAA AAAATCAGGA AATTCTGGAT 
GACACAGCTA AAAATCTTCG TGTTATGCTA GAATCTGAGA GAGAACAGAA TGTCAAGAAT 
CAGGACTTGA TATCAGAAAA CAAGAAATCT ATAGAGAAGT TAAAGGATGT TATTTCAATG 
AATGCCTCAG AATTTTCAGA GGTTCAGATT GCACTTAATG AAGCTAAGCT TAGTGAAGAG 
AAGGTGAAGT CTGAATGCCA TCGGGTTCAA GAAGAAAATG CTAGGCTTAA GAAGAAAAAA 
GAGCAGTTGC AGCAGGAAAT CGAAGACTGG AGTAAATTAC ATGCTGAGCT CAGTGAGCAA 
ATCAAATCAT TTGAGAAGTC TCAGAAAGAT TTGGAAGTAG CTCTTACTCA CAAGGATGAT 
AATATTAATG CTTTGACTAA CTGCATTACA CAGTTGAATC TGTTAGAGTG TGAATCTGAA 
TCTGAGGGTC AAAATAAAGG TGGAAATGAT TCAGATGAAT TAGCAAATGG AGAAGTGGGA 
GGTGACCGGA ATGAGAAGAT GAAAAATCAA ATTAAGCAGA TGATGGATGT CTCTCGGACA 
CAGACTGCAA TATCGGTAGT TGAAGAGGAT CTAAAGCTTT TACAGCTTAA GCTAAGAGCC 
TCCGTGTCCA CTAAATGTAA CCTGGAAGAC CAGGTAAAGA AATTGGAAGA TGACCGCAAC 
TCACTACAAG CTGCCAAAGC TGGACTGGAA GATGAATGCA AAACCTTGAG GCAGAAAGTG 
GAGATTCTGA ATGAGCTCTA TCAGCAGAAG GAGATGGCTT TGCAAAAGAA ACTGAGTCAA 
GAAGAGTATG AACGGCAAGA AAGAGAGCAC AGGCTGTCAG CTGCAGATGA AAAGGCAGTT 
TCGGCTGCAG AGGAAGTAAA AACTTACAAG CGGAGAATTG AAGAAATGGA GGATGAATTA 
CAGAAGACAG AGCGGTCATT TAAAAACCAG ATCGCTACCC ATGAGAAGAA AGCTCATGAA 
AACTGGCTCA AAGCTCGTGC TGCAGAAAGA GCTATAGCTG AAGAGAAAAG GGAAGCTGCC 
AATTTGAGAC ACAAATTATT AGAATTAACA CAAAAGATGG CAATGCTGCA AGAAGAACCT 
GTGATTGTAA AACCAATGCC AGGAAAACCA AATACACAAA ACCCTCCACG GAGAGGTCCT 
CTGAGCCAGA ATGGCTCTTT TGGCCCATCC CCTGTGAGTG GTGGAGAATG CTCCCCTCCA 
TTGACAGTGG AGCCACCCGT GAGACCTCTC TCTGCTACTC TCAATCGAAG AGATATGCCT 
AGAAGTGAAT TTGGATCAGT GGACGGGCCT CTACCTCATC CTCGATGGTC AGCTGAGGCA 
TCTGGGAAAC CCTCTCCTTC TGATCCAGGA TCTGGTACAG CTACCATGAT GAACAGCAGC 
TCAAGAGGCT CTTCCCCTAC CAGGGTACTC GATGAAGGCA AGGTTAATAT GGCTCCAAAA 
GGGCCCCCTC CTTTCCCAGG AGTCCCTCTC ATGAGCACCC CCATGGGAGG CCCTGTACCA 
CCACCCATTC GATATGGACC ACCACCTCAG CTCTGCGGAC CTTTTGGGCC TCGGCCACTT 
CCTCCACCCT TTGGCCCTGG TATGCGTCCA CCACTAGGCT TAAGAGAATT TGCACCAGGC 
GTTCCACCAG GAAGACGGGA CCTGCCTCTC CACCCTCGGG GATTTTTACC TGGACACGCA 
CCATTTAGAC CTTTAGGTTC ACTTGGCCCA AGAGAGTACT TTATTCCTGG TACCCGATTA 
CCACCCCCAA CCCATGGTCC CCAGGAATAC CCACCACCAC CTGCTGTAAG AGACTTACTG 
CCGTCAGGCT CTAGAGATGA GCCTCCACCT GCCTCTCAGA GCACTAGCCA GGACTGTTCA 
CAGGCTTTAA AACAGAGCCC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( NM_198551.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_940953.2 Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 1, mRNA.

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CloneID	OHu13395
Clone ID Related Accession (Same CDS sequence)	NM_198551.3 , NM_198551.4
Accession Version	NM_198551.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	transport and Golgi organization protein 1 homolog isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592148.7, DA040423.1, AK096526.1, D87742.1, BP423646.1 and CK724924.1. On Nov 23, 2018 this sequence version replaced NM_198551.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.49234.1, SRR1803617.42223.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000344922.10/ ENSP00000340900.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGCGG CGCCTGGGCT GCTCGTCTGG CTGCTCGTGC TCCGGCTGCC CTGGCGGGTG 
CCGGGCCAGC TGGACCCCAG CACTGGCCGG CGGTTCTCGG AGCACAAACT CTGCGCGGAC 
GACGAATGCA GCATGTTAAT GTACCGCGGT GAGGCTCTTG AAGATTTCAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGTGAT CCTGTATATG TTTACTATAA ACTGGCAAGA 
GGATGGCCTG AAGTTTGGGC TGGAAGTGTT GGACGCACTT TTGGATATTT TCCAAAAGAT 
TTAATCCAGG TAGTTCATGA ATATACCAAA GAAGAGCTAC AAGTTCCAAC AGATGAGACG 
GATTTTGTTT GTTTTGATGG AGGAAGAGAT GATTTTCATA ATTATAATGT AGAAGAACTT 
TTAGGGTTTT TGGAACTGTA CAATTCTGCA GCTACAGATT CTGAGAAAGC TGTAGAAAAA 
ACTTTACAGG ATATGGAAAA AAACCCTGAA TTATCTAAGG AAAGGGAACC TGAACCTGAA 
CCAGTAGAAG CCAACTCAGA GGAAAGTGAT AGTGTATTCT CAGAAAACAC TGAGGATCTT 
CAGGAACAGT TTACAACTCA GAAGCACCAC TCCCATGCAA ACAGCCAAGC AAATCATGCT 
CAGGGAGAGC AGGCTTCATT TGAATCTTTT GAAGAAATGC TGCAAGATAA ACTAAAAGTG 
CCAGAAAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCAAATGA ACAGGATAAG 
ATTGATGCCT ATAAACTTTT GAAAAAAGAA ATGACTCTAG ACTTGAAAAC CAAATTTGGC 
TCAACAGCTG ATGCACTTGT ATCTGATGAT GAGACAACCA GACTCGTTAC TTCATTAGAA 
GATGATTTTG ATGAGGAATT GGATACTGAG TATTATGCAG TTGGAAAGGA AGATGAGGAG 
AACCAAGAAG ACTTTGATGA GTTGCCATTA CTTACCTTTA CAGATGGGGA AGATATGAAA 
ACTCCAGCAA AGTCTGGCGT TGAGAAATAT CCAACAGATA AAGAGCAGAA TTCAAATGAA 
GAGGACAAGG TTCAGCTAAC TGTGCCCCCT GGCATCAAAA ATGATGATAA AAATATACTA 
ACAACCTGGG GGGACACTAT CTTCTCTATT GTCACAGGAG GTGAAGAAAC AAGAGATACG 
ATGGATTTAG AGAGCTCTAG TTCAGAGGAA GAAAAAGAAG ATGATGATGA TGCATTAGTC 
CCAGATAGCA AACAGGGGAA ACCACAGTCA GCAACAGATT ATAGTGACCC TGACAATGTA 
GATGATGGTC TTTTTATTGT AGACATTCCT AAAACAAATA ATGACAAAGA AGTAAACGCA 
GAACATCACA TTAAAGGAAA AGGGAGGGGA GTTCAGGAAT CCAAGAGGGG CCTGGTACAA 
GATAAGACAG AATTAGAGGA TGAAAATCAA GAAGGCATGA CTGTGCACAG TTCTGTTCAC 
AGCAATAACC TCAACTCTAT GCCAGCTGCT GAAAAGGGTA AAGACACATT AAAATCAGCT 
TATGATGATA CAGAAAATGA CCTAAAAGGA GCAGCTATTC ATATCTCAAA AGGAATGCTC 
CACGAAGAAA AGCCTGGAGA GCAGATTTTG GAAGGTGGCT CAGAGAGTGA ATCTGCACAG 
AAAGCTGCAG GGAATCAAAT GAATGACAGA AAGATTCAAC AGGAATCCCT GGGTAGTGCA 
CCACTCATGG GAGATGACCA CCCTAACGCA TCCAGAGACA GTGTGGAGGG AGACGCTTTG 
GTAAATGGGG CCAAACTGCA CACGCTTTCA GTGGAGCATC AACGTGAGGA ATTGAAAGAG 
GAATTAGTTC TTAAAACTCA AAACCAACCT AGATTCTCCT CTCCAGATGA GATTGATTTG 
CCCAGAGAAC TGGAAGACGA GGTTCCCATT CTGGGAAGAA ATCTTCCCTG GCAACAAGAA 
AGAGATGTGG CTGCCACAGC CAGTAAGCAA ATGAGTGAGA AGATAAGGCT CTCTGAGGGA 
GAAGCCAAAG AGGACTCCTT GGATGAAGAG TTTTTTCATC ACAAGGCAAT GCAGGGCACA 
GAGGTAGGAC AGACAGACCA AACTGACAGC ACAGGAGGAC CAGCTTTCCT TTCTAAAGTA 
GAAGAGGATG ATTATCCCTC TGAAGAACTA CTAGAGGATG AAAACGCTAT AAATGCAAAA 
CGGTCTAAAG AAAAAAACCC TGGGAATCAG GGCAGGCAGT TTGATGTTAA TCTGCAAGTC 
CCTGACAGAG CAGTTTTAGG GACCATTCAT CCAGATCCAG AAATTGAAGA AAGCAAGCAA 
GAAACTAGTA TGATTTTGGA TAGCGAAAAA ACAAGTGAGA CTGCTGCCAA AGGGGTCAAC 
ACAGGAGGCA GGGAACCAAA TACAATGGTG GAAAAAGAAC GCCCTCTGGC AGATAAGAAA 
GCACAGAGAC CATTTGAACG AAGTGACTTT TCTGACAGCA TAAAAATTCA GACTCCAGAA 
TTAGGTGAAG TGTTTCAGAA TAAAGATTCT GATTATCTGA AGAACGACAA CCCTGAGGAA 
CATCTGAAGA CCTCAGGGCT TGCAGGGGAG CCTGAGGGAG AACTCTCAAA AGAGGACCAT 
GAGAACACAG AGAAGTACAT GGGCACAGAA AGCCAGGGGT CTGCTGCTGC AGAACCTGAA 
GATGACTCGT TCCACTGGAC TCCACATACA AGTGTAGAGC CAGGGCATAG TGACAAGAGG 
GAGGACTTAC TTATCATAAG CAGCTTCTTT AAAGAACAAC AGTCTTTGCA GCGGTTCCAG 
AAGTACTTTA ATGTCCATGA GCTGGAAGCC TTGCTACAAG AAATGTCATC AAAACTGAAG 
TCAGCGCAGC AGGAGAGCCT GCCCTATAAT ATGGAAAAAG TCCTAGATAA GGTCTTCCGT 
GCTTCTGAGT CACAAATTCT GAGCATAGCA GAAAAAATGC TTGATACTCG TGTGGCTGAA 
AATAGAGATC TGGGAATGAA CGAAAATAAC ATATTTGAAG AGGCTGCAGT GCTTGATGAC 
ATTCAAGACC TCATCTATTT TGTCAGGTAC AAGCACTCCA CAGCAGAGGA GACAGCCACA 
CTGGTGATGG CACCACCTCT AGAGGAAGGC TTGGGTGGAG CAATGGAAGA GATGCAACCA 
CTGCATGAAG ATAATTTCTC ACGAGAGAAG ACAGCAGAAC TTAATGTGCA GGTTCCTGAA 
GAACCCACCC ACTTGGACCA ACGTGTGATT GGGGACACTC ATGCCTCAGA AGTGTCACAG 
AAGCCAAATA CTGAGAAAGA CCTGGACCCA GGGCCAGTTA CAACAGAAGA CACTCCTATG 
GATGCTATTG ATGCAAACAA GCAACCAGAG ACAGCCGCCG AAGAGCCGGC AAGTGTCACA 
CCTTTGGAAA ACGCAATCCT TCTAATATAT TCATTCATGT TTTATTTAAC TAAGTCGCTA 
GTTGCTACAT TGCCTGATGA TGTTCAGCCT GGGCCTGATT TTTATGGACT GCCATGGAAA 
CCTGTATTTA TCACTGCCTT CTTGGGAATT GCTTCGTTTG CCATTTTCTT ATGGAGAACT 
GTCCTTGTTG TGAAGGATAG AGTATATCAA GTCACGGAAC AGCAAATTTC TGAGAAGTTG 
AAGACTATCA TGAAAGAAAA TACAGAACTT GTACAAAAAT TGTCAAATTA TGAACAGAAG 
ATCAAGGAAT CAAAGAAACA TGTTCAGGAA ACCAGGAAAC AAAATATGAT TCTCTCTGAT 
GAAGCAATTA AATATAAGGA TAAAATCAAG ACACTTGAAA AAAATCAGGA AATTCTGGAT 
GACACAGCTA AAAATCTTCG TGTTATGCTA GAATCTGAGA GAGAACAGAA TGTCAAGAAT 
CAGGACTTGA TATCAGAAAA CAAGAAATCT ATAGAGAAGT TAAAGGATGT TATTTCAATG 
AATGCCTCAG AATTTTCAGA GGTTCAGATT GCACTTAATG AAGCTAAGCT TAGTGAAGAG 
AAGGTGAAGT CTGAATGCCA TCGGGTTCAA GAAGAAAATG CTAGGCTTAA GAAGAAAAAA 
GAGCAGTTGC AGCAGGAAAT CGAAGACTGG AGTAAATTAC ATGCTGAGCT CAGTGAGCAA 
ATCAAATCAT TTGAGAAGTC TCAGAAAGAT TTGGAAGTAG CTCTTACTCA CAAGGATGAT 
AATATTAATG CTTTGACTAA CTGCATTACA CAGTTGAATC TGTTAGAGTG TGAATCTGAA 
TCTGAGGGTC AAAATAAAGG TGGAAATGAT TCAGATGAAT TAGCAAATGG AGAAGTGGGA 
GGTGACCGGA ATGAGAAGAT GAAAAATCAA ATTAAGCAGA TGATGGATGT CTCTCGGACA 
CAGACTGCAA TATCGGTAGT TGAAGAGGAT CTAAAGCTTT TACAGCTTAA GCTAAGAGCC 
TCCGTGTCCA CTAAATGTAA CCTGGAAGAC CAGGTAAAGA AATTGGAAGA TGACCGCAAC 
TCACTACAAG CTGCCAAAGC TGGACTGGAA GATGAATGCA AAACCTTGAG GCAGAAAGTG 
GAGATTCTGA ATGAGCTCTA TCAGCAGAAG GAGATGGCTT TGCAAAAGAA ACTGAGTCAA 
GAAGAGTATG AACGGCAAGA AAGAGAGCAC AGGCTGTCAG CTGCAGATGA AAAGGCAGTT 
TCGGCTGCAG AGGAAGTAAA AACTTACAAG CGGAGAATTG AAGAAATGGA GGATGAATTA 
CAGAAGACAG AGCGGTCATT TAAAAACCAG ATCGCTACCC ATGAGAAGAA AGCTCATGAA 
AACTGGCTCA AAGCTCGTGC TGCAGAAAGA GCTATAGCTG AAGAGAAAAG GGAAGCTGCC 
AATTTGAGAC ACAAATTATT AGAATTAACA CAAAAGATGG CAATGCTGCA AGAAGAACCT 
GTGATTGTAA AACCAATGCC AGGAAAACCA AATACACAAA ACCCTCCACG GAGAGGTCCT 
CTGAGCCAGA ATGGCTCTTT TGGCCCATCC CCTGTGAGTG GTGGAGAATG CTCCCCTCCA 
TTGACAGTGG AGCCACCCGT GAGACCTCTC TCTGCTACTC TCAATCGAAG AGATATGCCT 
AGAAGTGAAT TTGGATCAGT GGACGGGCCT CTACCTCATC CTCGATGGTC AGCTGAGGCA 
TCTGGGAAAC CCTCTCCTTC TGATCCAGGA TCTGGTACAG CTACCATGAT GAACAGCAGC 
TCAAGAGGCT CTTCCCCTAC CAGGGTACTC GATGAAGGCA AGGTTAATAT GGCTCCAAAA 
GGGCCCCCTC CTTTCCCAGG AGTCCCTCTC ATGAGCACCC CCATGGGAGG CCCTGTACCA 
CCACCCATTC GATATGGACC ACCACCTCAG CTCTGCGGAC CTTTTGGGCC TCGGCCACTT 
CCTCCACCCT TTGGCCCTGG TATGCGTCCA CCACTAGGCT TAAGAGAATT TGCACCAGGC 
GTTCCACCAG GAAGACGGGA CCTGCCTCTC CACCCTCGGG GATTTTTACC TGGACACGCA 
CCATTTAGAC CTTTAGGTTC ACTTGGCCCA AGAGAGTACT TTATTCCTGG TACCCGATTA 
CCACCCCCAA CCCATGGTCC CCAGGAATAC CCACCACCAC CTGCTGTAAG AGACTTACTG 
CCGTCAGGCT CTAGAGATGA GCCTCCACCT GCCTCTCAGA GCACTAGCCA GGACTGTTCA 
CAGGCTTTAA AACAGAGCCC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_940953.2
CDS	15..5738
Translation	MAAAPGLLVWLLVLRLPWRVPGQLDPSTGRRFSEHKLCADDECSMLMYRGEALEDFTGPDCRFVNFKKGDPVYVYYKLARGWPEVWAGSVGRTFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFHNYNVEELLGFLELYNSAATDSEKAVEKTLQDMEKNPELSKEREPEPEPVEANSEESDSVFSENTEDLQEQFTTQKHHSHANSQANHAQGEQASFESFEEMLQDKLKVPESENNKTSNSSQVSNEQDKIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDTEYYAVGKEDEENQEDFDELPLLTFTDGEDMKTPAKSGVEKYPTDKEQNSNEEDKVQLTVPPGIKNDDKNILTTWGDTIFSIVTGGEETRDTMDLESSSSEEEKEDDDDALVPDSKQGKPQSATDYSDPDNVDDGLFIVDIPKTNNDKEVNAEHHIKGKGRGVQESKRGLVQDKTELEDENQEGMTVHSSVHSNNLNSMPAAEKGKDTLKSAYDDTENDLKGAAIHISKGMLHEEKPGEQILEGGSESESAQKAAGNQMNDRKIQQESLGSAPLMGDDHPNASRDSVEGDALVNGAKLHTLSVEHQREELKEELVLKTQNQPRFSSPDEIDLPRELEDEVPILGRNLPWQQERDVAATASKQMSEKIRLSEGEAKEDSLDEEFFHHKAMQGTEVGQTDQTDSTGGPAFLSKVEEDDYPSEELLEDENAINAKRSKEKNPGNQGRQFDVNLQVPDRAVLGTIHPDPEIEESKQETSMILDSEKTSETAAKGVNTGGREPNTMVEKERPLADKKAQRPFERSDFSDSIKIQTPELGEVFQNKDSDYLKNDNPEEHLKTSGLAGEPEGELSKEDHENTEKYMGTESQGSAAAEPEDDSFHWTPHTSVEPGHSDKREDLLIISSFFKEQQSLQRFQKYFNVHELEALLQEMSSKLKSAQQESLPYNMEKVLDKVFRASESQILSIAEKMLDTRVAENRDLGMNENNIFEEAAVLDDIQDLIYFVRYKHSTAEETATLVMAPPLEEGLGGAMEEMQPLHEDNFSREKTAELNVQVPEEPTHLDQRVIGDTHASEVSQKPNTEKDLDPGPVTTEDTPMDAIDANKQPETAAEEPASVTPLENAILLIYSFMFYLTKSLVATLPDDVQPGPDFYGLPWKPVFITAFLGIASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLISENKKSIEKLKDVISMNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREHRLSAADEKAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGKPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

Target ORF information:

RefSeq Version	NM_198551.4
Organism	Homo sapiens(human)
Definition	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198551.4

ORF Insert Sequence:

ATGGCTGCGG CGCCTGGGCT GCTCGTCTGG CTGCTCGTGC TCCGGCTGCC CTGGCGGGTG 
CCGGGCCAGC TGGACCCCAG CACTGGCCGG CGGTTCTCGG AGCACAAACT CTGCGCGGAC 
GACGAATGCA GCATGTTAAT GTACCGCGGT GAGGCTCTTG AAGATTTCAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGTGAT CCTGTATATG TTTACTATAA ACTGGCAAGA 
GGATGGCCTG AAGTTTGGGC TGGAAGTGTT GGACGCACTT TTGGATATTT TCCAAAAGAT 
TTAATCCAGG TAGTTCATGA ATATACCAAA GAAGAGCTAC AAGTTCCAAC AGATGAGACG 
GATTTTGTTT GTTTTGATGG AGGAAGAGAT GATTTTCATA ATTATAATGT AGAAGAACTT 
TTAGGGTTTT TGGAACTGTA CAATTCTGCA GCTACAGATT CTGAGAAAGC TGTAGAAAAA 
ACTTTACAGG ATATGGAAAA AAACCCTGAA TTATCTAAGG AAAGGGAACC TGAACCTGAA 
CCAGTAGAAG CCAACTCAGA GGAAAGTGAT AGTGTATTCT CAGAAAACAC TGAGGATCTT 
CAGGAACAGT TTACAACTCA GAAGCACCAC TCCCATGCAA ACAGCCAAGC AAATCATGCT 
CAGGGAGAGC AGGCTTCATT TGAATCTTTT GAAGAAATGC TGCAAGATAA ACTAAAAGTG 
CCAGAAAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCAAATGA ACAGGATAAG 
ATTGATGCCT ATAAACTTTT GAAAAAAGAA ATGACTCTAG ACTTGAAAAC CAAATTTGGC 
TCAACAGCTG ATGCACTTGT ATCTGATGAT GAGACAACCA GACTCGTTAC TTCATTAGAA 
GATGATTTTG ATGAGGAATT GGATACTGAG TATTATGCAG TTGGAAAGGA AGATGAGGAG 
AACCAAGAAG ACTTTGATGA GTTGCCATTA CTTACCTTTA CAGATGGGGA AGATATGAAA 
ACTCCAGCAA AGTCTGGCGT TGAGAAATAT CCAACAGATA AAGAGCAGAA TTCAAATGAA 
GAGGACAAGG TTCAGCTAAC TGTGCCCCCT GGCATCAAAA ATGATGATAA AAATATACTA 
ACAACCTGGG GGGACACTAT CTTCTCTATT GTCACAGGAG GTGAAGAAAC AAGAGATACG 
ATGGATTTAG AGAGCTCTAG TTCAGAGGAA GAAAAAGAAG ATGATGATGA TGCATTAGTC 
CCAGATAGCA AACAGGGGAA ACCACAGTCA GCAACAGATT ATAGTGACCC TGACAATGTA 
GATGATGGTC TTTTTATTGT AGACATTCCT AAAACAAATA ATGACAAAGA AGTAAACGCA 
GAACATCACA TTAAAGGAAA AGGGAGGGGA GTTCAGGAAT CCAAGAGGGG CCTGGTACAA 
GATAAGACAG AATTAGAGGA TGAAAATCAA GAAGGCATGA CTGTGCACAG TTCTGTTCAC 
AGCAATAACC TCAACTCTAT GCCAGCTGCT GAAAAGGGTA AAGACACATT AAAATCAGCT 
TATGATGATA CAGAAAATGA CCTAAAAGGA GCAGCTATTC ATATCTCAAA AGGAATGCTC 
CACGAAGAAA AGCCTGGAGA GCAGATTTTG GAAGGTGGCT CAGAGAGTGA ATCTGCACAG 
AAAGCTGCAG GGAATCAAAT GAATGACAGA AAGATTCAAC AGGAATCCCT GGGTAGTGCA 
CCACTCATGG GAGATGACCA CCCTAACGCA TCCAGAGACA GTGTGGAGGG AGACGCTTTG 
GTAAATGGGG CCAAACTGCA CACGCTTTCA GTGGAGCATC AACGTGAGGA ATTGAAAGAG 
GAATTAGTTC TTAAAACTCA AAACCAACCT AGATTCTCCT CTCCAGATGA GATTGATTTG 
CCCAGAGAAC TGGAAGACGA GGTTCCCATT CTGGGAAGAA ATCTTCCCTG GCAACAAGAA 
AGAGATGTGG CTGCCACAGC CAGTAAGCAA ATGAGTGAGA AGATAAGGCT CTCTGAGGGA 
GAAGCCAAAG AGGACTCCTT GGATGAAGAG TTTTTTCATC ACAAGGCAAT GCAGGGCACA 
GAGGTAGGAC AGACAGACCA AACTGACAGC ACAGGAGGAC CAGCTTTCCT TTCTAAAGTA 
GAAGAGGATG ATTATCCCTC TGAAGAACTA CTAGAGGATG AAAACGCTAT AAATGCAAAA 
CGGTCTAAAG AAAAAAACCC TGGGAATCAG GGCAGGCAGT TTGATGTTAA TCTGCAAGTC 
CCTGACAGAG CAGTTTTAGG GACCATTCAT CCAGATCCAG AAATTGAAGA AAGCAAGCAA 
GAAACTAGTA TGATTTTGGA TAGCGAAAAA ACAAGTGAGA CTGCTGCCAA AGGGGTCAAC 
ACAGGAGGCA GGGAACCAAA TACAATGGTG GAAAAAGAAC GCCCTCTGGC AGATAAGAAA 
GCACAGAGAC CATTTGAACG AAGTGACTTT TCTGACAGCA TAAAAATTCA GACTCCAGAA 
TTAGGTGAAG TGTTTCAGAA TAAAGATTCT GATTATCTGA AGAACGACAA CCCTGAGGAA 
CATCTGAAGA CCTCAGGGCT TGCAGGGGAG CCTGAGGGAG AACTCTCAAA AGAGGACCAT 
GAGAACACAG AGAAGTACAT GGGCACAGAA AGCCAGGGGT CTGCTGCTGC AGAACCTGAA 
GATGACTCGT TCCACTGGAC TCCACATACA AGTGTAGAGC CAGGGCATAG TGACAAGAGG 
GAGGACTTAC TTATCATAAG CAGCTTCTTT AAAGAACAAC AGTCTTTGCA GCGGTTCCAG 
AAGTACTTTA ATGTCCATGA GCTGGAAGCC TTGCTACAAG AAATGTCATC AAAACTGAAG 
TCAGCGCAGC AGGAGAGCCT GCCCTATAAT ATGGAAAAAG TCCTAGATAA GGTCTTCCGT 
GCTTCTGAGT CACAAATTCT GAGCATAGCA GAAAAAATGC TTGATACTCG TGTGGCTGAA 
AATAGAGATC TGGGAATGAA CGAAAATAAC ATATTTGAAG AGGCTGCAGT GCTTGATGAC 
ATTCAAGACC TCATCTATTT TGTCAGGTAC AAGCACTCCA CAGCAGAGGA GACAGCCACA 
CTGGTGATGG CACCACCTCT AGAGGAAGGC TTGGGTGGAG CAATGGAAGA GATGCAACCA 
CTGCATGAAG ATAATTTCTC ACGAGAGAAG ACAGCAGAAC TTAATGTGCA GGTTCCTGAA 
GAACCCACCC ACTTGGACCA ACGTGTGATT GGGGACACTC ATGCCTCAGA AGTGTCACAG 
AAGCCAAATA CTGAGAAAGA CCTGGACCCA GGGCCAGTTA CAACAGAAGA CACTCCTATG 
GATGCTATTG ATGCAAACAA GCAACCAGAG ACAGCCGCCG AAGAGCCGGC AAGTGTCACA 
CCTTTGGAAA ACGCAATCCT TCTAATATAT TCATTCATGT TTTATTTAAC TAAGTCGCTA 
GTTGCTACAT TGCCTGATGA TGTTCAGCCT GGGCCTGATT TTTATGGACT GCCATGGAAA 
CCTGTATTTA TCACTGCCTT CTTGGGAATT GCTTCGTTTG CCATTTTCTT ATGGAGAACT 
GTCCTTGTTG TGAAGGATAG AGTATATCAA GTCACGGAAC AGCAAATTTC TGAGAAGTTG 
AAGACTATCA TGAAAGAAAA TACAGAACTT GTACAAAAAT TGTCAAATTA TGAACAGAAG 
ATCAAGGAAT CAAAGAAACA TGTTCAGGAA ACCAGGAAAC AAAATATGAT TCTCTCTGAT 
GAAGCAATTA AATATAAGGA TAAAATCAAG ACACTTGAAA AAAATCAGGA AATTCTGGAT 
GACACAGCTA AAAATCTTCG TGTTATGCTA GAATCTGAGA GAGAACAGAA TGTCAAGAAT 
CAGGACTTGA TATCAGAAAA CAAGAAATCT ATAGAGAAGT TAAAGGATGT TATTTCAATG 
AATGCCTCAG AATTTTCAGA GGTTCAGATT GCACTTAATG AAGCTAAGCT TAGTGAAGAG 
AAGGTGAAGT CTGAATGCCA TCGGGTTCAA GAAGAAAATG CTAGGCTTAA GAAGAAAAAA 
GAGCAGTTGC AGCAGGAAAT CGAAGACTGG AGTAAATTAC ATGCTGAGCT CAGTGAGCAA 
ATCAAATCAT TTGAGAAGTC TCAGAAAGAT TTGGAAGTAG CTCTTACTCA CAAGGATGAT 
AATATTAATG CTTTGACTAA CTGCATTACA CAGTTGAATC TGTTAGAGTG TGAATCTGAA 
TCTGAGGGTC AAAATAAAGG TGGAAATGAT TCAGATGAAT TAGCAAATGG AGAAGTGGGA 
GGTGACCGGA ATGAGAAGAT GAAAAATCAA ATTAAGCAGA TGATGGATGT CTCTCGGACA 
CAGACTGCAA TATCGGTAGT TGAAGAGGAT CTAAAGCTTT TACAGCTTAA GCTAAGAGCC 
TCCGTGTCCA CTAAATGTAA CCTGGAAGAC CAGGTAAAGA AATTGGAAGA TGACCGCAAC 
TCACTACAAG CTGCCAAAGC TGGACTGGAA GATGAATGCA AAACCTTGAG GCAGAAAGTG 
GAGATTCTGA ATGAGCTCTA TCAGCAGAAG GAGATGGCTT TGCAAAAGAA ACTGAGTCAA 
GAAGAGTATG AACGGCAAGA AAGAGAGCAC AGGCTGTCAG CTGCAGATGA AAAGGCAGTT 
TCGGCTGCAG AGGAAGTAAA AACTTACAAG CGGAGAATTG AAGAAATGGA GGATGAATTA 
CAGAAGACAG AGCGGTCATT TAAAAACCAG ATCGCTACCC ATGAGAAGAA AGCTCATGAA 
AACTGGCTCA AAGCTCGTGC TGCAGAAAGA GCTATAGCTG AAGAGAAAAG GGAAGCTGCC 
AATTTGAGAC ACAAATTATT AGAATTAACA CAAAAGATGG CAATGCTGCA AGAAGAACCT 
GTGATTGTAA AACCAATGCC AGGAAAACCA AATACACAAA ACCCTCCACG GAGAGGTCCT 
CTGAGCCAGA ATGGCTCTTT TGGCCCATCC CCTGTGAGTG GTGGAGAATG CTCCCCTCCA 
TTGACAGTGG AGCCACCCGT GAGACCTCTC TCTGCTACTC TCAATCGAAG AGATATGCCT 
AGAAGTGAAT TTGGATCAGT GGACGGGCCT CTACCTCATC CTCGATGGTC AGCTGAGGCA 
TCTGGGAAAC CCTCTCCTTC TGATCCAGGA TCTGGTACAG CTACCATGAT GAACAGCAGC 
TCAAGAGGCT CTTCCCCTAC CAGGGTACTC GATGAAGGCA AGGTTAATAT GGCTCCAAAA 
GGGCCCCCTC CTTTCCCAGG AGTCCCTCTC ATGAGCACCC CCATGGGAGG CCCTGTACCA 
CCACCCATTC GATATGGACC ACCACCTCAG CTCTGCGGAC CTTTTGGGCC TCGGCCACTT 
CCTCCACCCT TTGGCCCTGG TATGCGTCCA CCACTAGGCT TAAGAGAATT TGCACCAGGC 
GTTCCACCAG GAAGACGGGA CCTGCCTCTC CACCCTCGGG GATTTTTACC TGGACACGCA 
CCATTTAGAC CTTTAGGTTC ACTTGGCCCA AGAGAGTACT TTATTCCTGG TACCCGATTA 
CCACCCCCAA CCCATGGTCC CCAGGAATAC CCACCACCAC CTGCTGTAAG AGACTTACTG 
CCGTCAGGCT CTAGAGATGA GCCTCCACCT GCCTCTCAGA GCACTAGCCA GGACTGTTCA 
CAGGCTTTAA AACAGAGCCC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( NM_001324062.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001310991.1 Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 3, mRNA.

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CloneID	OHu34358
Clone ID Related Accession (Same CDS sequence)	NM_001324062.1
Accession Version	NM_001324062.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5796bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-09
Organism	Homo sapiens(human)
Product	transport and Golgi organization protein 1 homolog isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL592148.7 and KF459535.1. On Apr 26, 2016 this sequence version replaced XM_005273121.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.33501.1, SRR1660809.142172.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGCGG CGCCTGGGCT GCTCGTCTGG CTGCTCGTGC TCCGGCTGCC CTGGCGGGTG 
CCGGGCCAGC TGGACCCCAG CACTGGCCGG CGGTTCTCGG AGCACAAACT CTGCGCGGAC 
GACGAATGCA GCATGTTAAT GTACCGCGGT GAGGCTCTTG AAGATTTCAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGTGAT CCTGTATATG TTTACTATAA ACTGGCAAGA 
GGATGGCCTG AAGTTTGGGC TGGAAGTGTT GGACGCACTT TTGGATATTT TCCAAAAGAT 
TTAATCCAGG TAGTTCATGA ATATACCAAA GAAGAGCTAC AAGTTCCAAC AGATGAGACG 
GATTTTGTTT GTTTTGATGG AGGAAGAGAT GATTTTCATA ATTATAATGT AGAAGAACTT 
TTAGGGTTTT TGGAACTGTA CAATTCTGCA GCTACAGATT CTGAGAAAGC TGTAGAAAAA 
ACTTTACAGG ATATGGAAAA AAACCCTGAA TTATCTAAGG AAAGGGAACC TGAACCTGAA 
CCAGTAGAAG CCAACTCAGA GGAAAGTGAT AGTGTATTCT CAGAAAACAC TGAGGATCTT 
CAGGAACAGT TTACAACTCA GAAGCACCAC TCCCATGCAA ACAGCCAAGC AAATCATGCT 
CAGGGAGAGC AGGCTTCATT TGAATCTTTT GAAGAAATGC TGCAAGATAA ACTAAAAGTG 
CCAGAAAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCAAATGA ACAGGATAAG 
ATTGATGCCT ATAAACTTTT GAAAAAAGAA ATGACTCTAG ACTTGAAAAC CAAATTTGGC 
TCAACAGCTG ATGCACTTGT ATCTGATGAT GAGACAACCA GACTCGTTAC TTCATTAGAA 
GATGATTTTG ATGAGGAATT GGATACTGAG TATTATGCAG TTGGAAAGGA AGATGAGGAG 
AACCAAGAAG ACTTTGATGA GTTGCCATTA CTTACCTTTA CAGATGGGGA AGATATGAAA 
ACTCCAGCAA AGTCTGGCGT TGAGAAATAT CCAACAGATA AAGAGCAGAA TTCAAATGAA 
GAGGACAAGG TTCAGCTAAC TGTGCCCCCT GGCATCAAAA ATGATGATAA AAATATACTA 
ACAACCTGGG GGGACACTAT CTTCTCTATT GTCACAGGAG GTGAAGAAAC AAGAGATACG 
ATGGATTTAG AGAGCTCTAG TTCAGAGGAA GAAAAAGAAG ATGATGATGA TGCATTAGTC 
CCAGATAGCA AACAGGGGAA ACCACAGTCA GCAACAGATT ATAGTGACCC TGACAATGTA 
GATGATGGTC TTTTTATTGT AGACATTCCT AAAACAAATA ATGACAAAGA AGTAAACGCA 
GAACATCACA TTAAAGGAAA AGGGAGGGGA GTTCAGGAAT CCAAGAGGGG CCTGGTACAA 
GATAAGACAG AATTAGAGGA TGAAAATCAA GAAGGCATGA CTGTGCACAG TTCTGTTCAC 
AGCAATAACC TCAACTCTAT GCCAGCTGCT GAAAAGGGTA AAGACACATT AAAATCAGCT 
TATGATGATA CAGAAAATGA CCTAAAAGGA GCAGCTATTC ATATCTCAAA AGGAATGCTC 
CACGAAGAAA AGCCTGGAGA GCAGATTTTG GAAGGTGGCT CAGAGAGTGA ATCTGCACAG 
AAAGCTGCAG GGAATCAAAT GAATGACAGA AAGATTCAAC AGGAATCCCT GGGTAGTGCA 
CCACTCATGG GAGATGACCA CCCTAACGCA TCCAGAGACA GTGTGGAGGG AGACGCTTTG 
GTAAATGGGG CCAAACTGCA CACGCTTTCA GTGGAGCATC AACGTGAGGA ATTGAAAGAG 
GAATTAGTTC TTAAAACTCA AAACCAACCT AGATTCTCCT CTCCAGATGA GATTGATTTG 
CCCAGAGAAC TGGAAGACGA GGTTCCCATT CTGGGAAGAA ATCTTCCCTG GCAACAAGAA 
AGAGATGTGG CTGCCACAGC CAGTAAGCAA ATGAGTGAGA AGATAAGGCT CTCTGAGGGA 
GAAGCCAAAG AGGACTCCTT GGATGAAGAG TTTTTTCATC ACAAGGCAAT GCAGGGCACA 
GAGGTAGGAC AGACAGACCA AACTGACAGC ACAGGAGGAC CAGCTTTCCT TTCTAAAGTA 
GAAGAGGATG ATTATCCCTC TGAAGAACTA CTAGAGGATG AAAACGCTAT AAATGCAAAA 
CGGTCTAAAG AAAAAAACCC TGGGAATCAG GGCAGGCAGT TTGATGTTAA TCTGCAAGTC 
CCTGACAGAG CAGTTTTAGG GACCATTCAT CCAGATCCAG AAATTGAAGA AAGCAAGCAA 
GAAACTAGTA TGATTTTGGA TAGCGAAAAA ACAAGTGAGA CTGCTGCCAA AGGGGTCAAC 
ACAGGAGGCA GGGAACCAAA TACAATGGTG GAAAAAGAAC GCCCTCTGGC AGATAAGAAA 
GCACAGAGAC CATTTGAACG AAGTGACTTT TCTGACAGCA TAAAAATTCA GACTCCAGAA 
TTAGGTGAAG TGTTTCAGAA TAAAGATTCT GATTATCTGA AGAACGACAA CCCTGAGGAA 
CATCTGAAGA CCTCAGGGCT TGCAGGGGAG CCTGAGGGAG AACTCTCAAA AGAGGACCAT 
GAGAACACAG AGAAGTACAT GGGCACAGAA AGCCAGGGGT CTGCTGCTGC AGAACCTGAA 
GATGACTCGT TCCACTGGAC TCCACATACA AGTGTAGAGC CAGGGCATAG TGACAAGAGG 
GAGGACTTAC TTATCATAAG CAGCTTCTTT AAAGAACAAC AGTCTTTGCA GCGGTTCCAG 
AAGTACTTTA ATGTCCATGA GCTGGAAGCC TTGCTACAAG AAATGTCATC AAAACTGAAG 
TCAGCGCAGC AGGAGAGCCT GCCCTATAAT ATGGAAAAAG TCCTAGATAA GGTCTTCCGT 
GCTTCTGAGT CACAAATTCT GAGCATAGCA GAAAAAATGC TTGATACTCG TGTGGCTGAA 
AATAGAGATC TGGGAATGAA CGAAAATAAC ATATTTGAAG AGGCTGCAGT GCTTGATGAC 
ATTCAAGACC TCATCTATTT TGTCAGGTAC AAGCACTCCA CAGCAGAGGA GACAGCCACA 
CTGGTGATGG CACCACCTCT AGAGGAAGGC TTGGGTGGAG CAATGGAAGA GATGCAACCA 
CTGCATGAAG ATAATTTCTC ACGAGAGAAG ACAGCAGAAC TTAATGTGCA GGTTCCTGAA 
GAACCCACCC ACTTGGACCA ACGTGTGATT GGGGACACTC ATGCCTCAGA AGTGTCACAG 
AAGCCAAATA CTGAGAAAGA CCTGGACCCA GGGCCAGTTA CAACAGAAGA CACTCCTATG 
GATGCTATTG ATGCAAACAA GCAACCAGAG ACAGCCGCCG AAGAGCCGGC AAGTGTCACA 
CCTTTGGAAA ACGCAATCCT TCTAATATAT TCATTCATGT TTTATTTAAC TAAGTCGCTA 
GTTGCTACAT TGCCTGATGA TGTTCAGCCT GGGCCTGATT TTTATGGACT GCCATGGAAA 
CCTGTATTTA TCACTGCCTT CTTGGGAATT GCTTCGTTTG CCATTTTCTT ATGGAGAACT 
GTCCTTGTTG TGAAGGATAG AGTATATCAA GTCACGGAAC AGCAAATTTC TGAGAAGTTG 
AAGACTATCA TGAAAGAAAA TACAGAACTT GTACAAAAAT TGTCAAATTA TGAACAGAAG 
ATCAAGGAAT CAAAGAAACA TGTTCAGGAA ACCAGGAAAC AAAATATGAT TCTCTCTGAT 
GAAGCAATTA AATATAAGGA TAAAATCAAG ACACTTGAAA AAAATCAGGA AATTCTGGAT 
GACACAGCTA AAAATCTTCG TGTTATGCTA GAATCTGAGA GAGAACAGAA TGTCAAGAAT 
CAGGACTTGA TATCAGAAAA CAAGAAATCT ATAGAGAAGT TAAAGGATGT TATTTCAATG 
AATGCCTCAG AATTTTCAGA GGTTCAGATT GCACTTAATG AAGCTAAGCT TAGTGAAGAG 
AAGGTGAAGT CTGAATGCCA TCGGGTTCAA GAAGAAAATG CTAGGCTTAA GAAGAAAAAA 
GAGCAGTTGC AGCAGGAAAT CGAAGACTGG AGTAAATTAC ATGCTGAGCT CAGTGAGCAA 
ATCAAATCAT TTGAGAAGTC TCAGAAAGAT TTGGAAGTAG CTCTTACTCA CAAGGATGAT 
AATATTAATG CTTTGACTAA CTGCATTACA CAGTTGAATC TGTTAGAGTG TGAATCTGAA 
TCTGAGGGTC AAAATAAAGG TGGAAATGAT TCAGATGAAT TAGCAAATGG AGAAGTGGGA 
GGTGACCGGA ATGAGAAGAT GAAAAATCAA ATTAAGCAGA TGATGGATGT CTCTCGGACA 
CAGACTGCAA TATCGGTAGT TGAAGAGGAT CTAAAGCTTT TACAGCTTAA GCTAAGAGCC 
TCCGTGTCCA CTAAATGTAA CCTGGAAGAC CAGGTAAAGA AATTGGAAGA TGACCGCAAC 
TCACTACAAG CTGCCAAAGC TGGACTGGAA GATGAATGCA AAACCTTGAG GCAGAAAGTG 
GAGATTCTGA ATGAGCTCTA TCAGCAGAAG GAGATGGCTT TGCAAAAGAA ACTGAGTCAA 
GAAGAGTATG AACGGCAAGA AAGAGAGCAC AGGCTGTCAG CTGCAGATGA AAAGGCAGTT 
TCGGCTGCAG AGGAAGTAAA AACTTACAAG CGGAGAATTG AAGAAATGGA GGATGAATTA 
CAGAAGACAG AGCGGTCATT TAAAAACCAG ATCGCTACCC ATGAGAAGAA AGCTCATGAA 
AACTGGCTCA AAGCTCGTGC TGCAGAAAGA GCTATAGCTG AAGAGAAAAG GGAAGCTGCC 
AATTTGAGAC ACAAATTATT AGAATTAACA CAAAAGATGG CAATGCTGCA AGAAGAACCT 
GTGATTGTAA AACCAATGCC AGGAAAACCA AATACACAAA ACCCTCCACG GAGAGGTCCT 
CTGAGCCAGA ATGGCTCTTT TGGCCCATCC CCTGTGAGTG GTGGAGAATG CTCCCCTCCA 
TTGACAGTGG AGCCACCCGT GAGACCTCTC TCTGCTACTC TCAATCGAAG AGATATGCCT 
AGAAGTGAAT TTGGATCAGT GGACGGGCCT CTACCTCATC CTCGATGGTC AGCTGAGGCA 
TCTGGGAAAC CCTCTCCTTC TGATCCAGGA TCTGGTACAG CTACCATGAT GAACAGCAGC 
TCAAGAGGCT CTTCCCCTAC CAGGGTACTC GATGAAGGCA AGCAAACTGT TCTCCAAGAG 
CCTGAAGTCC CCTCAGTTCC CAGCATTACA TCTTTGGCTG AGCGTCCAGT AGCAGTTAAT 
ATGGCTCCAA AAGGGCCCCC TCCTTTCCCA GGAGTCCCTC TCATGAGCAC CCCCATGGGA 
GGCCCTGTAC CACCACCCAT TCGATATGGA CCACCACCTC AGCTCTGCGG ACCTTTTGGG 
CCTCGGCCAC TTCCTCCACC CTTTGGCCCT GGTATGCGTC CACCACTAGG CTTAAGAGAA 
TTTGCACCAG GCGTTCCACC AGGAAGACGG GACCTGCCTC TCCACCCTCG GGGATTTTTA 
CCTGGACACG CACCATTTAG ACCTTTAGGT TCACTTGGCC CAAGAGAGTA CTTTATTCCT 
GGTACCCGAT TACCACCCCC AACCCATGGT CCCCAGGAAT ACCCACCACC ACCTGCTGTA 
AGAGACTTAC TGCCGTCAGG CTCTAGAGAT GAGCCTCCAC CTGCCTCTCA GAGCACTAGC 
CAGGACTGTT CACAGGCTTT AAAACAGAGC CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001310991.1
CDS	10..5805
Translation	MAAAPGLLVWLLVLRLPWRVPGQLDPSTGRRFSEHKLCADDECSMLMYRGEALEDFTGPDCRFVNFKKGDPVYVYYKLARGWPEVWAGSVGRTFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFHNYNVEELLGFLELYNSAATDSEKAVEKTLQDMEKNPELSKEREPEPEPVEANSEESDSVFSENTEDLQEQFTTQKHHSHANSQANHAQGEQASFESFEEMLQDKLKVPESENNKTSNSSQVSNEQDKIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDTEYYAVGKEDEENQEDFDELPLLTFTDGEDMKTPAKSGVEKYPTDKEQNSNEEDKVQLTVPPGIKNDDKNILTTWGDTIFSIVTGGEETRDTMDLESSSSEEEKEDDDDALVPDSKQGKPQSATDYSDPDNVDDGLFIVDIPKTNNDKEVNAEHHIKGKGRGVQESKRGLVQDKTELEDENQEGMTVHSSVHSNNLNSMPAAEKGKDTLKSAYDDTENDLKGAAIHISKGMLHEEKPGEQILEGGSESESAQKAAGNQMNDRKIQQESLGSAPLMGDDHPNASRDSVEGDALVNGAKLHTLSVEHQREELKEELVLKTQNQPRFSSPDEIDLPRELEDEVPILGRNLPWQQERDVAATASKQMSEKIRLSEGEAKEDSLDEEFFHHKAMQGTEVGQTDQTDSTGGPAFLSKVEEDDYPSEELLEDENAINAKRSKEKNPGNQGRQFDVNLQVPDRAVLGTIHPDPEIEESKQETSMILDSEKTSETAAKGVNTGGREPNTMVEKERPLADKKAQRPFERSDFSDSIKIQTPELGEVFQNKDSDYLKNDNPEEHLKTSGLAGEPEGELSKEDHENTEKYMGTESQGSAAAEPEDDSFHWTPHTSVEPGHSDKREDLLIISSFFKEQQSLQRFQKYFNVHELEALLQEMSSKLKSAQQESLPYNMEKVLDKVFRASESQILSIAEKMLDTRVAENRDLGMNENNIFEEAAVLDDIQDLIYFVRYKHSTAEETATLVMAPPLEEGLGGAMEEMQPLHEDNFSREKTAELNVQVPEEPTHLDQRVIGDTHASEVSQKPNTEKDLDPGPVTTEDTPMDAIDANKQPETAAEEPASVTPLENAILLIYSFMFYLTKSLVATLPDDVQPGPDFYGLPWKPVFITAFLGIASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLISENKKSIEKLKDVISMNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREHRLSAADEKAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGKPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKQTVLQEPEVPSVPSITSLAERPVAVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

Target ORF information:

RefSeq Version	NM_001324062.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MIA SH3 domain ER export factor 3 (MIA3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001324062.1

ORF Insert Sequence:

ATGGCTGCGG CGCCTGGGCT GCTCGTCTGG CTGCTCGTGC TCCGGCTGCC CTGGCGGGTG 
CCGGGCCAGC TGGACCCCAG CACTGGCCGG CGGTTCTCGG AGCACAAACT CTGCGCGGAC 
GACGAATGCA GCATGTTAAT GTACCGCGGT GAGGCTCTTG AAGATTTCAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGTGAT CCTGTATATG TTTACTATAA ACTGGCAAGA 
GGATGGCCTG AAGTTTGGGC TGGAAGTGTT GGACGCACTT TTGGATATTT TCCAAAAGAT 
TTAATCCAGG TAGTTCATGA ATATACCAAA GAAGAGCTAC AAGTTCCAAC AGATGAGACG 
GATTTTGTTT GTTTTGATGG AGGAAGAGAT GATTTTCATA ATTATAATGT AGAAGAACTT 
TTAGGGTTTT TGGAACTGTA CAATTCTGCA GCTACAGATT CTGAGAAAGC TGTAGAAAAA 
ACTTTACAGG ATATGGAAAA AAACCCTGAA TTATCTAAGG AAAGGGAACC TGAACCTGAA 
CCAGTAGAAG CCAACTCAGA GGAAAGTGAT AGTGTATTCT CAGAAAACAC TGAGGATCTT 
CAGGAACAGT TTACAACTCA GAAGCACCAC TCCCATGCAA ACAGCCAAGC AAATCATGCT 
CAGGGAGAGC AGGCTTCATT TGAATCTTTT GAAGAAATGC TGCAAGATAA ACTAAAAGTG 
CCAGAAAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCAAATGA ACAGGATAAG 
ATTGATGCCT ATAAACTTTT GAAAAAAGAA ATGACTCTAG ACTTGAAAAC CAAATTTGGC 
TCAACAGCTG ATGCACTTGT ATCTGATGAT GAGACAACCA GACTCGTTAC TTCATTAGAA 
GATGATTTTG ATGAGGAATT GGATACTGAG TATTATGCAG TTGGAAAGGA AGATGAGGAG 
AACCAAGAAG ACTTTGATGA GTTGCCATTA CTTACCTTTA CAGATGGGGA AGATATGAAA 
ACTCCAGCAA AGTCTGGCGT TGAGAAATAT CCAACAGATA AAGAGCAGAA TTCAAATGAA 
GAGGACAAGG TTCAGCTAAC TGTGCCCCCT GGCATCAAAA ATGATGATAA AAATATACTA 
ACAACCTGGG GGGACACTAT CTTCTCTATT GTCACAGGAG GTGAAGAAAC AAGAGATACG 
ATGGATTTAG AGAGCTCTAG TTCAGAGGAA GAAAAAGAAG ATGATGATGA TGCATTAGTC 
CCAGATAGCA AACAGGGGAA ACCACAGTCA GCAACAGATT ATAGTGACCC TGACAATGTA 
GATGATGGTC TTTTTATTGT AGACATTCCT AAAACAAATA ATGACAAAGA AGTAAACGCA 
GAACATCACA TTAAAGGAAA AGGGAGGGGA GTTCAGGAAT CCAAGAGGGG CCTGGTACAA 
GATAAGACAG AATTAGAGGA TGAAAATCAA GAAGGCATGA CTGTGCACAG TTCTGTTCAC 
AGCAATAACC TCAACTCTAT GCCAGCTGCT GAAAAGGGTA AAGACACATT AAAATCAGCT 
TATGATGATA CAGAAAATGA CCTAAAAGGA GCAGCTATTC ATATCTCAAA AGGAATGCTC 
CACGAAGAAA AGCCTGGAGA GCAGATTTTG GAAGGTGGCT CAGAGAGTGA ATCTGCACAG 
AAAGCTGCAG GGAATCAAAT GAATGACAGA AAGATTCAAC AGGAATCCCT GGGTAGTGCA 
CCACTCATGG GAGATGACCA CCCTAACGCA TCCAGAGACA GTGTGGAGGG AGACGCTTTG 
GTAAATGGGG CCAAACTGCA CACGCTTTCA GTGGAGCATC AACGTGAGGA ATTGAAAGAG 
GAATTAGTTC TTAAAACTCA AAACCAACCT AGATTCTCCT CTCCAGATGA GATTGATTTG 
CCCAGAGAAC TGGAAGACGA GGTTCCCATT CTGGGAAGAA ATCTTCCCTG GCAACAAGAA 
AGAGATGTGG CTGCCACAGC CAGTAAGCAA ATGAGTGAGA AGATAAGGCT CTCTGAGGGA 
GAAGCCAAAG AGGACTCCTT GGATGAAGAG TTTTTTCATC ACAAGGCAAT GCAGGGCACA 
GAGGTAGGAC AGACAGACCA AACTGACAGC ACAGGAGGAC CAGCTTTCCT TTCTAAAGTA 
GAAGAGGATG ATTATCCCTC TGAAGAACTA CTAGAGGATG AAAACGCTAT AAATGCAAAA 
CGGTCTAAAG AAAAAAACCC TGGGAATCAG GGCAGGCAGT TTGATGTTAA TCTGCAAGTC 
CCTGACAGAG CAGTTTTAGG GACCATTCAT CCAGATCCAG AAATTGAAGA AAGCAAGCAA 
GAAACTAGTA TGATTTTGGA TAGCGAAAAA ACAAGTGAGA CTGCTGCCAA AGGGGTCAAC 
ACAGGAGGCA GGGAACCAAA TACAATGGTG GAAAAAGAAC GCCCTCTGGC AGATAAGAAA 
GCACAGAGAC CATTTGAACG AAGTGACTTT TCTGACAGCA TAAAAATTCA GACTCCAGAA 
TTAGGTGAAG TGTTTCAGAA TAAAGATTCT GATTATCTGA AGAACGACAA CCCTGAGGAA 
CATCTGAAGA CCTCAGGGCT TGCAGGGGAG CCTGAGGGAG AACTCTCAAA AGAGGACCAT 
GAGAACACAG AGAAGTACAT GGGCACAGAA AGCCAGGGGT CTGCTGCTGC AGAACCTGAA 
GATGACTCGT TCCACTGGAC TCCACATACA AGTGTAGAGC CAGGGCATAG TGACAAGAGG 
GAGGACTTAC TTATCATAAG CAGCTTCTTT AAAGAACAAC AGTCTTTGCA GCGGTTCCAG 
AAGTACTTTA ATGTCCATGA GCTGGAAGCC TTGCTACAAG AAATGTCATC AAAACTGAAG 
TCAGCGCAGC AGGAGAGCCT GCCCTATAAT ATGGAAAAAG TCCTAGATAA GGTCTTCCGT 
GCTTCTGAGT CACAAATTCT GAGCATAGCA GAAAAAATGC TTGATACTCG TGTGGCTGAA 
AATAGAGATC TGGGAATGAA CGAAAATAAC ATATTTGAAG AGGCTGCAGT GCTTGATGAC 
ATTCAAGACC TCATCTATTT TGTCAGGTAC AAGCACTCCA CAGCAGAGGA GACAGCCACA 
CTGGTGATGG CACCACCTCT AGAGGAAGGC TTGGGTGGAG CAATGGAAGA GATGCAACCA 
CTGCATGAAG ATAATTTCTC ACGAGAGAAG ACAGCAGAAC TTAATGTGCA GGTTCCTGAA 
GAACCCACCC ACTTGGACCA ACGTGTGATT GGGGACACTC ATGCCTCAGA AGTGTCACAG 
AAGCCAAATA CTGAGAAAGA CCTGGACCCA GGGCCAGTTA CAACAGAAGA CACTCCTATG 
GATGCTATTG ATGCAAACAA GCAACCAGAG ACAGCCGCCG AAGAGCCGGC AAGTGTCACA 
CCTTTGGAAA ACGCAATCCT TCTAATATAT TCATTCATGT TTTATTTAAC TAAGTCGCTA 
GTTGCTACAT TGCCTGATGA TGTTCAGCCT GGGCCTGATT TTTATGGACT GCCATGGAAA 
CCTGTATTTA TCACTGCCTT CTTGGGAATT GCTTCGTTTG CCATTTTCTT ATGGAGAACT 
GTCCTTGTTG TGAAGGATAG AGTATATCAA GTCACGGAAC AGCAAATTTC TGAGAAGTTG 
AAGACTATCA TGAAAGAAAA TACAGAACTT GTACAAAAAT TGTCAAATTA TGAACAGAAG 
ATCAAGGAAT CAAAGAAACA TGTTCAGGAA ACCAGGAAAC AAAATATGAT TCTCTCTGAT 
GAAGCAATTA AATATAAGGA TAAAATCAAG ACACTTGAAA AAAATCAGGA AATTCTGGAT 
GACACAGCTA AAAATCTTCG TGTTATGCTA GAATCTGAGA GAGAACAGAA TGTCAAGAAT 
CAGGACTTGA TATCAGAAAA CAAGAAATCT ATAGAGAAGT TAAAGGATGT TATTTCAATG 
AATGCCTCAG AATTTTCAGA GGTTCAGATT GCACTTAATG AAGCTAAGCT TAGTGAAGAG 
AAGGTGAAGT CTGAATGCCA TCGGGTTCAA GAAGAAAATG CTAGGCTTAA GAAGAAAAAA 
GAGCAGTTGC AGCAGGAAAT CGAAGACTGG AGTAAATTAC ATGCTGAGCT CAGTGAGCAA 
ATCAAATCAT TTGAGAAGTC TCAGAAAGAT TTGGAAGTAG CTCTTACTCA CAAGGATGAT 
AATATTAATG CTTTGACTAA CTGCATTACA CAGTTGAATC TGTTAGAGTG TGAATCTGAA 
TCTGAGGGTC AAAATAAAGG TGGAAATGAT TCAGATGAAT TAGCAAATGG AGAAGTGGGA 
GGTGACCGGA ATGAGAAGAT GAAAAATCAA ATTAAGCAGA TGATGGATGT CTCTCGGACA 
CAGACTGCAA TATCGGTAGT TGAAGAGGAT CTAAAGCTTT TACAGCTTAA GCTAAGAGCC 
TCCGTGTCCA CTAAATGTAA CCTGGAAGAC CAGGTAAAGA AATTGGAAGA TGACCGCAAC 
TCACTACAAG CTGCCAAAGC TGGACTGGAA GATGAATGCA AAACCTTGAG GCAGAAAGTG 
GAGATTCTGA ATGAGCTCTA TCAGCAGAAG GAGATGGCTT TGCAAAAGAA ACTGAGTCAA 
GAAGAGTATG AACGGCAAGA AAGAGAGCAC AGGCTGTCAG CTGCAGATGA AAAGGCAGTT 
TCGGCTGCAG AGGAAGTAAA AACTTACAAG CGGAGAATTG AAGAAATGGA GGATGAATTA 
CAGAAGACAG AGCGGTCATT TAAAAACCAG ATCGCTACCC ATGAGAAGAA AGCTCATGAA 
AACTGGCTCA AAGCTCGTGC TGCAGAAAGA GCTATAGCTG AAGAGAAAAG GGAAGCTGCC 
AATTTGAGAC ACAAATTATT AGAATTAACA CAAAAGATGG CAATGCTGCA AGAAGAACCT 
GTGATTGTAA AACCAATGCC AGGAAAACCA AATACACAAA ACCCTCCACG GAGAGGTCCT 
CTGAGCCAGA ATGGCTCTTT TGGCCCATCC CCTGTGAGTG GTGGAGAATG CTCCCCTCCA 
TTGACAGTGG AGCCACCCGT GAGACCTCTC TCTGCTACTC TCAATCGAAG AGATATGCCT 
AGAAGTGAAT TTGGATCAGT GGACGGGCCT CTACCTCATC CTCGATGGTC AGCTGAGGCA 
TCTGGGAAAC CCTCTCCTTC TGATCCAGGA TCTGGTACAG CTACCATGAT GAACAGCAGC 
TCAAGAGGCT CTTCCCCTAC CAGGGTACTC GATGAAGGCA AGCAAACTGT TCTCCAAGAG 
CCTGAAGTCC CCTCAGTTCC CAGCATTACA TCTTTGGCTG AGCGTCCAGT AGCAGTTAAT 
ATGGCTCCAA AAGGGCCCCC TCCTTTCCCA GGAGTCCCTC TCATGAGCAC CCCCATGGGA 
GGCCCTGTAC CACCACCCAT TCGATATGGA CCACCACCTC AGCTCTGCGG ACCTTTTGGG 
CCTCGGCCAC TTCCTCCACC CTTTGGCCCT GGTATGCGTC CACCACTAGG CTTAAGAGAA 
TTTGCACCAG GCGTTCCACC AGGAAGACGG GACCTGCCTC TCCACCCTCG GGGATTTTTA 
CCTGGACACG CACCATTTAG ACCTTTAGGT TCACTTGGCC CAAGAGAGTA CTTTATTCCT 
GGTACCCGAT TACCACCCCC AACCCATGGT CCCCAGGAAT ACCCACCACC ACCTGCTGTA 
AGAGACTTAC TGCCGTCAGG CTCTAGAGAT GAGCCTCCAC CTGCCTCTCA GAGCACTAGC 
CAGGACTGTT CACAGGCTTT AAAACAGAGC CCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_006711304.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006711367.2 Homo sapiens melanoma inhibitory activity family member 3 (MIA3), transcript variant X1, mRNA.

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CloneID	OHu87370
Clone ID Related Accession (Same CDS sequence)	XM_006711304.3
Accession Version	XM_006711304.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	melanoma inhibitory activity protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006711304.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCCGG CGCTCCGCCT GGTCCCTGCC CGCGTCCCTG CCCCCGTCCC TGCCCCCGTC 
CCTGCCCCCG TCCCCGCCCC GTCCCCGCCC CCGCCCCCGC CCCCGTCCCC GCCCCCGTCC 
CCGCCCCGTC CCCGCCCCAG CCCCCGCCCC AGCCCCCCCG TCCCCGCCCC GTCCCCGCCC 
CCGTCCCGCC CCTCTGCGCC CGCCCCGGCC TCCTCCTCCG CGTCACCGGC TCCCCGAGGT 
GACCACAACA TGGCTGCGGC GCCTGGGCTG CTCGTCTGGC TGCTCGTGCT CCGGCTGCCC 
TGGCGGGTGC CGGGCCAGCT GGACCCCAGC ACTGGCCGGC GGTTCTCGGA GCACAAACTC 
TGCGCGGACG ACGAATGCAG CATGTTAATG TACCGCGGTG AGGCTCTTGA AGATTTCACA 
GGCCCGGATT GTCGTTTTGT GAATTTTAAA AAAGGTGATC CTGTATATGT TTACTATAAA 
CTGGCAAGAG GATGGCCTGA AGTTTGGGCT GGAAGTGTTG GACGCACTTT TGGATATTTT 
CCAAAAGATT TAATCCAGGT AGTTCATGAA TATACCAAAG AAGAGCTACA AGTTCCAACA 
GATGAGACGG ATTTTGTTTG TTTTGATGGA GGAAGAGATG ATTTTCATAA TTATAATGTA 
GAAGAACTTT TAGGGTTTTT GGAACTGTAC AATTCTGCAG CTACAGATTC TGAGAAAGCT 
GTAGAAAAAA CTTTACAGGA TATGGAAAAA AACCCTGAAT TATCTAAGGA AAGGGAACCT 
GAACCTGAAC CAGTAGAAGC CAACTCAGAG GAAAGTGATA GTGTATTCTC AGAAAACACT 
GAGGATCTTC AGGAACAGTT TACAACTCAG AAGCACCACT CCCATGCAAA CAGCCAAGCA 
AATCATGCTC AGGGAGAGCA GGCTTCATTT GAATCTTTTG AAGAAATGCT GCAAGATAAA 
CTAAAAGTGC CAGAAAGTGA AAACAACAAA ACCAGCAATA GTTCTCAGGT CTCAAATGAA 
CAGGATAAGA TTGATGCCTA TAAACTTTTG AAAAAAGAAA TGACTCTAGA CTTGAAAACC 
AAATTTGGCT CAACAGCTGA TGCACTTGTA TCTGATGATG AGACAACCAG ACTCGTTACT 
TCATTAGAAG ATGATTTTGA TGAGGAATTG GATACTGAGT ATTATGCAGT TGGAAAGGAA 
GATGAGGAGA ACCAAGAAGA CTTTGATGAG TTGCCATTAC TTACCTTTAC AGATGGGGAA 
GATATGAAAA CTCCAGCAAA GTCTGGCGTT GAGAAATATC CAACAGATAA AGAGCAGAAT 
TCAAATGAAG AGGACAAGGT TCAGCTAACT GTGCCCCCTG GCATCAAAAA TGATGATAAA 
AATATACTAA CAACCTGGGG GGACACTATC TTCTCTATTG TCACAGGAGG TGAAGAAACA 
AGAGATACGA TGGATTTAGA GAGCTCTAGT TCAGAGGAAG AAAAAGAAGA TGATGATGAT 
GCATTAGTCC CAGATAGCAA ACAGGGGAAA CCACAGTCAG CAACAGATTA TAGTGACCCT 
GACAATGTAG ATGATGGTCT TTTTATTGTA GACATTCCTA AAACAAATAA TGACAAAGAA 
GTAAACGCAG AACATCACAT TAAAGGAAAA GGGAGGGGAG TTCAGGAATC CAAGAGGGGC 
CTGGTACAAG ATAAGACAGA ATTAGAGGAT GAAAATCAAG AAGGCATGAC TGTGCACAGT 
TCTGTTCACA GCAATAACCT CAACTCTATG CCAGCTGCTG AAAAGGGTAA AGACACATTA 
AAATCAGCTT ATGATGATAC AGAAAATGAC CTAAAAGGAG CAGCTATTCA TATCTCAAAA 
GGAATGCTCC ACGAAGAAAA GCCTGGAGAG CAGATTTTGG AAGGTGGCTC AGAGAGTGAA 
TCTGCACAGA AAGCTGCAGG GAATCAAATG AATGACAGAA AGATTCAACA GGAATCCCTG 
GGTAGTGCAC CACTCATGGG AGATGACCAC CCTAACGCAT CCAGAGACAG TGTGGAGGGA 
GACGCTTTGG TAAATGGGGC CAAACTGCAC ACGCTTTCAG TGGAGCATCA ACGTGAGGAA 
TTGAAAGAGG AATTAGTTCT TAAAACTCAA AACCAACCTA GATTCTCCTC TCCAGATGAG 
ATTGATTTGC CCAGAGAACT GGAAGACGAG GTTCCCATTC TGGGAAGAAA TCTTCCCTGG 
CAACAAGAAA GAGATGTGGC TGCCACAGCC AGTAAGCAAA TGAGTGAGAA GATAAGGCTC 
TCTGAGGGAG AAGCCAAAGA GGACTCCTTG GATGAAGAGT TTTTTCATCA CAAGGCAATG 
CAGGGCACAG AGGTAGGACA GACAGACCAA ACTGACAGCA CAGGAGGACC AGCTTTCCTT 
TCTAAAGTAG AAGAGGATGA TTATCCCTCT GAAGAACTAC TAGAGGATGA AAACGCTATA 
AATGCAAAAC GGTCTAAAGA AAAAAACCCT GGGAATCAGG GCAGGCAGTT TGATGTTAAT 
CTGCAAGTCC CTGACAGAGC AGTTTTAGGG ACCATTCATC CAGATCCAGA AATTGAAGAA 
AGCAAGCAAG AAACTAGTAT GATTTTGGAT AGCGAAAAAA CAAGTGAGAC TGCTGCCAAA 
GGGGTCAACA CAGGAGGCAG GGAACCAAAT ACAATGGTGG AAAAAGAACG CCCTCTGGCA 
GATAAGAAAG CACAGAGACC ATTTGAACGA AGTGACTTTT CTGACAGCAT AAAAATTCAG 
ACTCCAGAAT TAGGTGAAGT GTTTCAGAAT AAAGATTCTG ATTATCTGAA GAACGACAAC 
CCTGAGGAAC ATCTGAAGAC CTCAGGGCTT GCAGGGGAGC CTGAGGGAGA ACTCTCAAAA 
GAGGACCATG AGAACACAGA GAAGTACATG GGCACAGAAA GCCAGGGGTC TGCTGCTGCA 
GAACCTGAAG ATGACTCGTT CCACTGGACT CCACATACAA GTGTAGAGCC AGGGCATAGT 
GACAAGAGGG AGGACTTACT TATCATAAGC AGCTTCTTTA AAGAACAACA GTCTTTGCAG 
CGGTTCCAGA AGTACTTTAA TGTCCATGAG CTGGAAGCCT TGCTACAAGA AATGTCATCA 
AAACTGAAGT CAGCGCAGCA GGAGAGCCTG CCCTATAATA TGGAAAAAGT CCTAGATAAG 
GTCTTCCGTG CTTCTGAGTC ACAAATTCTG AGCATAGCAG AAAAAATGCT TGATACTCGT 
GTGGCTGAAA ATAGAGATCT GGGAATGAAC GAAAATAACA TATTTGAAGA GGCTGCAGTG 
CTTGATGACA TTCAAGACCT CATCTATTTT GTCAGGTACA AGCACTCCAC AGCAGAGGAG 
ACAGCCACAC TGGTGATGGC ACCACCTCTA GAGGAAGGCT TGGGTGGAGC AATGGAAGAG 
ATGCAACCAC TGCATGAAGA TAATTTCTCA CGAGAGAAGA CAGCAGAACT TAATGTGCAG 
GTTCCTGAAG AACCCACCCA CTTGGACCAA CGTGTGATTG GGGACACTCA TGCCTCAGAA 
GTGTCACAGA AGCCAAATAC TGAGAAAGAC CTGGACCCAG GGCCAGTTAC AACAGAAGAC 
ACTCCTATGG ATGCTATTGA TGCAAACAAG CAACCAGAGA CAGCCGCCGA AGAGCCGGCA 
AGTGTCACAC CTTTGGAAAA CGCAATCCTT CTAATATATT CATTCATGTT TTATTTAACT 
AAGTCGCTAG TTGCTACATT GCCTGATGAT GTTCAGCCTG GGCCTGATTT TTATGGACTG 
CCATGGAAAC CTGTATTTAT CACTGCCTTC TTGGGAATTG CTTCGTTTGC CATTTTCTTA 
TGGAGAACTG TCCTTGTTGT GAAGGATAGA GTATATCAAG TCACGGAACA GCAAATTTCT 
GAGAAGTTGA AGACTATCAT GAAAGAAAAT ACAGAACTTG TACAAAAATT GTCAAATTAT 
GAACAGAAGA TCAAGGAATC AAAGAAACAT GTTCAGGAAA CCAGGAAACA AAATATGATT 
CTCTCTGATG AAGCAATTAA ATATAAGGAT AAAATCAAGA CACTTGAAAA AAATCAGGAA 
ATTCTGGATG ACACAGCTAA AAATCTTCGT GTTATGCTAG AATCTGAGAG AGAACAGAAT 
GTCAAGAATC AGGACTTGTT GCAGCAGGAA ATCGAAGACT GGAGTAAATT ACATGCTGAG 
CTCAGTGAGC AAATCAAATC ATTTGAGAAG TCTCAGAAAG ATTTGGAAGT AGCTCTTACT 
CACAAGGATG ATAATATTAA TGCTTTGACT AACTGCATTA CACAGTTGAA TCTGTTAGAG 
TGTGAATCTG AATCTGAGGG TCAAAATAAA GGTGGAAATG ATTCAGATGA ATTAGCAAAT 
GGAGAAGTGG GAGGTGACCG GAATGAGAAG ATGAAAAATC AAATTAAGCA GATGATGGAT 
GTCTCTCGGA CACAGACTGC AATATCGGTA GTTGAAGAGG ATCTAAAGCT TTTACAGCTT 
AAGCTAAGAG CCTCCGTGTC CACTAAATGT AACCTGGAAG ACCAGGTAAA GAAATTGGAA 
GATGACCGCA ACTCACTACA AGCTGCCAAA GCTGGACTGG AAGATGAATG CAAAACCTTG 
AGGCAGAAAG TGGAGATTCT GAATGAGCTC TATCAGCAGA AGGAGATGGC TTTGCAAAAG 
AAACTGAGTC AAGAAGAGTA TGAACGGCAA GAAAGAGAGC ACAGGCTGTC AGCTGCAGAT 
GAAAAGGCAG TTTCGGCTGC AGAGGAAGTA AAAACTTACA AGCGGAGAAT TGAAGAAATG 
GAGGATGAAT TACAGAAGAC AGAGCGGTCA TTTAAAAACC AGATCGCTAC CCATGAGAAG 
AAAGCTCATG AAAACTGGCT CAAAGCTCGT GCTGCAGAAA GAGCTATAGC TGAAGAGAAA 
AGGGAAGCTG CCAATTTGAG ACACAAATTA TTAGAATTAA CACAAAAGAT GGCAATGCTG 
CAAGAAGAAC CTGTGATTGT AAAACCAATG CCAGGAAAAC CAAATACACA AAACCCTCCA 
CGGAGAGGTC CTCTGAGCCA GAATGGCTCT TTTGGCCCAT CCCCTGTGAG TGGTGGAGAA 
TGCTCCCCTC CATTGACAGT GGAGCCACCC GTGAGACCTC TCTCTGCTAC TCTCAATCGA 
AGAGATATGC CTAGAAGTGA ATTTGGATCA GTGGACGGGC CTCTACCTCA TCCTCGATGG 
TCAGCTGAGG CATCTGGGAA ACCCTCTCCT TCTGATCCAG GATCTGGTAC AGCTACCATG 
ATGAACAGCA GCTCAAGAGG CTCTTCCCCT ACCAGGGTAC TCGATGAAGG CAAGCAAACT 
GTTCTCCAAG AGCCTGAAGT CCCCTCAGTT CCCAGCATTA CATCTTTGGC TGAGCGTCCA 
GTAGCAGTTA ATATGGCTCC AAAAGGGCCC CCTCCTTTCC CAGGAGTCCC TCTCATGAGC 
ACCCCCATGG GAGGCCCTGT ACCACCACCC ATTCGATATG GACCACCACC TCAGCTCTGC 
GGACCTTTTG GGCCTCGGCC ACTTCCTCCA CCCTTTGGCC CTGGTATGCG TCCACCACTA 
GGCTTAAGAG AATTTGCACC AGGCGTTCCA CCAGGAAGAC GGGACCTGCC TCTCCACCCT 
CGGGGATTTT TACCTGGACA CGCACCATTT AGACCTTTAG GTTCACTTGG CCCAAGAGAG 
TACTTTATTC CTGGTACCCG ATTACCACCC CCAACCCATG GTCCCCAGGA ATACCCACCA 
CCACCTGCTG TAAGAGACTT ACTGCCGTCA GGCTCTAGAG ATGAGCCTCC ACCTGCCTCT 
CAGAGCACTA GCCAGGACTG TTCACAGGCT TTAAAACAGA GCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006711367.2
CDS	73..5940
Translation	MPPALRLVPARVPAPVPAPVPAPVPAPSPPPPPPPSPPPSPPRPRPSPRPSPPVPAPSPPPSRPSAPAPASSSASPAPRGDHNMAAAPGLLVWLLVLRLPWRVPGQLDPSTGRRFSEHKLCADDECSMLMYRGEALEDFTGPDCRFVNFKKGDPVYVYYKLARGWPEVWAGSVGRTFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFHNYNVEELLGFLELYNSAATDSEKAVEKTLQDMEKNPELSKEREPEPEPVEANSEESDSVFSENTEDLQEQFTTQKHHSHANSQANHAQGEQASFESFEEMLQDKLKVPESENNKTSNSSQVSNEQDKIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDTEYYAVGKEDEENQEDFDELPLLTFTDGEDMKTPAKSGVEKYPTDKEQNSNEEDKVQLTVPPGIKNDDKNILTTWGDTIFSIVTGGEETRDTMDLESSSSEEEKEDDDDALVPDSKQGKPQSATDYSDPDNVDDGLFIVDIPKTNNDKEVNAEHHIKGKGRGVQESKRGLVQDKTELEDENQEGMTVHSSVHSNNLNSMPAAEKGKDTLKSAYDDTENDLKGAAIHISKGMLHEEKPGEQILEGGSESESAQKAAGNQMNDRKIQQESLGSAPLMGDDHPNASRDSVEGDALVNGAKLHTLSVEHQREELKEELVLKTQNQPRFSSPDEIDLPRELEDEVPILGRNLPWQQERDVAATASKQMSEKIRLSEGEAKEDSLDEEFFHHKAMQGTEVGQTDQTDSTGGPAFLSKVEEDDYPSEELLEDENAINAKRSKEKNPGNQGRQFDVNLQVPDRAVLGTIHPDPEIEESKQETSMILDSEKTSETAAKGVNTGGREPNTMVEKERPLADKKAQRPFERSDFSDSIKIQTPELGEVFQNKDSDYLKNDNPEEHLKTSGLAGEPEGELSKEDHENTEKYMGTESQGSAAAEPEDDSFHWTPHTSVEPGHSDKREDLLIISSFFKEQQSLQRFQKYFNVHELEALLQEMSSKLKSAQQESLPYNMEKVLDKVFRASESQILSIAEKMLDTRVAENRDLGMNENNIFEEAAVLDDIQDLIYFVRYKHSTAEETATLVMAPPLEEGLGGAMEEMQPLHEDNFSREKTAELNVQVPEEPTHLDQRVIGDTHASEVSQKPNTEKDLDPGPVTTEDTPMDAIDANKQPETAAEEPASVTPLENAILLIYSFMFYLTKSLVATLPDDVQPGPDFYGLPWKPVFITAFLGIASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREHRLSAADEKAVSAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHENWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPMPGKPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTVEPPVRPLSATLNRRDMPRSEFGSVDGPLPHPRWSAEASGKPSPSDPGSGTATMMNSSSRGSSPTRVLDEGKQTVLQEPEVPSVPSITSLAERPVAVNMAPKGPPPFPGVPLMSTPMGGPVPPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREFAPGVPPGRRDLPLHPRGFLPGHAPFRPLGSLGPREYFIPGTRLPPPTHGPQEYPPPPAVRDLLPSGSRDEPPPASQSTSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_006711304.3
Organism	Homo sapiens(human)
Definition	Homo sapiens melanoma inhibitory activity family member 3 (MIA3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006711304.3

ORF Insert Sequence:

ATGCCCCCGG CGCTCCGCCT GGTCCCTGCC CGCGTCCCTG CCCCCGTCCC TGCCCCCGTC 
CCTGCCCCCG TCCCCGCCCC GTCCCCGCCC CCGCCCCCGC CCCCGTCCCC GCCCCCGTCC 
CCGCCCCGTC CCCGCCCCAG CCCCCGCCCC AGCCCCCCCG TCCCCGCCCC GTCCCCGCCC 
CCGTCCCGCC CCTCTGCGCC CGCCCCGGCC TCCTCCTCCG CGTCACCGGC TCCCCGAGGT 
GACCACAACA TGGCTGCGGC GCCTGGGCTG CTCGTCTGGC TGCTCGTGCT CCGGCTGCCC 
TGGCGGGTGC CGGGCCAGCT GGACCCCAGC ACTGGCCGGC GGTTCTCGGA GCACAAACTC 
TGCGCGGACG ACGAATGCAG CATGTTAATG TACCGCGGTG AGGCTCTTGA AGATTTCACA 
GGCCCGGATT GTCGTTTTGT GAATTTTAAA AAAGGTGATC CTGTATATGT TTACTATAAA 
CTGGCAAGAG GATGGCCTGA AGTTTGGGCT GGAAGTGTTG GACGCACTTT TGGATATTTT 
CCAAAAGATT TAATCCAGGT AGTTCATGAA TATACCAAAG AAGAGCTACA AGTTCCAACA 
GATGAGACGG ATTTTGTTTG TTTTGATGGA GGAAGAGATG ATTTTCATAA TTATAATGTA 
GAAGAACTTT TAGGGTTTTT GGAACTGTAC AATTCTGCAG CTACAGATTC TGAGAAAGCT 
GTAGAAAAAA CTTTACAGGA TATGGAAAAA AACCCTGAAT TATCTAAGGA AAGGGAACCT 
GAACCTGAAC CAGTAGAAGC CAACTCAGAG GAAAGTGATA GTGTATTCTC AGAAAACACT 
GAGGATCTTC AGGAACAGTT TACAACTCAG AAGCACCACT CCCATGCAAA CAGCCAAGCA 
AATCATGCTC AGGGAGAGCA GGCTTCATTT GAATCTTTTG AAGAAATGCT GCAAGATAAA 
CTAAAAGTGC CAGAAAGTGA AAACAACAAA ACCAGCAATA GTTCTCAGGT CTCAAATGAA 
CAGGATAAGA TTGATGCCTA TAAACTTTTG AAAAAAGAAA TGACTCTAGA CTTGAAAACC 
AAATTTGGCT CAACAGCTGA TGCACTTGTA TCTGATGATG AGACAACCAG ACTCGTTACT 
TCATTAGAAG ATGATTTTGA TGAGGAATTG GATACTGAGT ATTATGCAGT TGGAAAGGAA 
GATGAGGAGA ACCAAGAAGA CTTTGATGAG TTGCCATTAC TTACCTTTAC AGATGGGGAA 
GATATGAAAA CTCCAGCAAA GTCTGGCGTT GAGAAATATC CAACAGATAA AGAGCAGAAT 
TCAAATGAAG AGGACAAGGT TCAGCTAACT GTGCCCCCTG GCATCAAAAA TGATGATAAA 
AATATACTAA CAACCTGGGG GGACACTATC TTCTCTATTG TCACAGGAGG TGAAGAAACA 
AGAGATACGA TGGATTTAGA GAGCTCTAGT TCAGAGGAAG AAAAAGAAGA TGATGATGAT 
GCATTAGTCC CAGATAGCAA ACAGGGGAAA CCACAGTCAG CAACAGATTA TAGTGACCCT 
GACAATGTAG ATGATGGTCT TTTTATTGTA GACATTCCTA AAACAAATAA TGACAAAGAA 
GTAAACGCAG AACATCACAT TAAAGGAAAA GGGAGGGGAG TTCAGGAATC CAAGAGGGGC 
CTGGTACAAG ATAAGACAGA ATTAGAGGAT GAAAATCAAG AAGGCATGAC TGTGCACAGT 
TCTGTTCACA GCAATAACCT CAACTCTATG CCAGCTGCTG AAAAGGGTAA AGACACATTA 
AAATCAGCTT ATGATGATAC AGAAAATGAC CTAAAAGGAG CAGCTATTCA TATCTCAAAA 
GGAATGCTCC ACGAAGAAAA GCCTGGAGAG CAGATTTTGG AAGGTGGCTC AGAGAGTGAA 
TCTGCACAGA AAGCTGCAGG GAATCAAATG AATGACAGAA AGATTCAACA GGAATCCCTG 
GGTAGTGCAC CACTCATGGG AGATGACCAC CCTAACGCAT CCAGAGACAG TGTGGAGGGA 
GACGCTTTGG TAAATGGGGC CAAACTGCAC ACGCTTTCAG TGGAGCATCA ACGTGAGGAA 
TTGAAAGAGG AATTAGTTCT TAAAACTCAA AACCAACCTA GATTCTCCTC TCCAGATGAG 
ATTGATTTGC CCAGAGAACT GGAAGACGAG GTTCCCATTC TGGGAAGAAA TCTTCCCTGG 
CAACAAGAAA GAGATGTGGC TGCCACAGCC AGTAAGCAAA TGAGTGAGAA GATAAGGCTC 
TCTGAGGGAG AAGCCAAAGA GGACTCCTTG GATGAAGAGT TTTTTCATCA CAAGGCAATG 
CAGGGCACAG AGGTAGGACA GACAGACCAA ACTGACAGCA CAGGAGGACC AGCTTTCCTT 
TCTAAAGTAG AAGAGGATGA TTATCCCTCT GAAGAACTAC TAGAGGATGA AAACGCTATA 
AATGCAAAAC GGTCTAAAGA AAAAAACCCT GGGAATCAGG GCAGGCAGTT TGATGTTAAT 
CTGCAAGTCC CTGACAGAGC AGTTTTAGGG ACCATTCATC CAGATCCAGA AATTGAAGAA 
AGCAAGCAAG AAACTAGTAT GATTTTGGAT AGCGAAAAAA CAAGTGAGAC TGCTGCCAAA 
GGGGTCAACA CAGGAGGCAG GGAACCAAAT ACAATGGTGG AAAAAGAACG CCCTCTGGCA 
GATAAGAAAG CACAGAGACC ATTTGAACGA AGTGACTTTT CTGACAGCAT AAAAATTCAG 
ACTCCAGAAT TAGGTGAAGT GTTTCAGAAT AAAGATTCTG ATTATCTGAA GAACGACAAC 
CCTGAGGAAC ATCTGAAGAC CTCAGGGCTT GCAGGGGAGC CTGAGGGAGA ACTCTCAAAA 
GAGGACCATG AGAACACAGA GAAGTACATG GGCACAGAAA GCCAGGGGTC TGCTGCTGCA 
GAACCTGAAG ATGACTCGTT CCACTGGACT CCACATACAA GTGTAGAGCC AGGGCATAGT 
GACAAGAGGG AGGACTTACT TATCATAAGC AGCTTCTTTA AAGAACAACA GTCTTTGCAG 
CGGTTCCAGA AGTACTTTAA TGTCCATGAG CTGGAAGCCT TGCTACAAGA AATGTCATCA 
AAACTGAAGT CAGCGCAGCA GGAGAGCCTG CCCTATAATA TGGAAAAAGT CCTAGATAAG 
GTCTTCCGTG CTTCTGAGTC ACAAATTCTG AGCATAGCAG AAAAAATGCT TGATACTCGT 
GTGGCTGAAA ATAGAGATCT GGGAATGAAC GAAAATAACA TATTTGAAGA GGCTGCAGTG 
CTTGATGACA TTCAAGACCT CATCTATTTT GTCAGGTACA AGCACTCCAC AGCAGAGGAG 
ACAGCCACAC TGGTGATGGC ACCACCTCTA GAGGAAGGCT TGGGTGGAGC AATGGAAGAG 
ATGCAACCAC TGCATGAAGA TAATTTCTCA CGAGAGAAGA CAGCAGAACT TAATGTGCAG 
GTTCCTGAAG AACCCACCCA CTTGGACCAA CGTGTGATTG GGGACACTCA TGCCTCAGAA 
GTGTCACAGA AGCCAAATAC TGAGAAAGAC CTGGACCCAG GGCCAGTTAC AACAGAAGAC 
ACTCCTATGG ATGCTATTGA TGCAAACAAG CAACCAGAGA CAGCCGCCGA AGAGCCGGCA 
AGTGTCACAC CTTTGGAAAA CGCAATCCTT CTAATATATT CATTCATGTT TTATTTAACT 
AAGTCGCTAG TTGCTACATT GCCTGATGAT GTTCAGCCTG GGCCTGATTT TTATGGACTG 
CCATGGAAAC CTGTATTTAT CACTGCCTTC TTGGGAATTG CTTCGTTTGC CATTTTCTTA 
TGGAGAACTG TCCTTGTTGT GAAGGATAGA GTATATCAAG TCACGGAACA GCAAATTTCT 
GAGAAGTTGA AGACTATCAT GAAAGAAAAT ACAGAACTTG TACAAAAATT GTCAAATTAT 
GAACAGAAGA TCAAGGAATC AAAGAAACAT GTTCAGGAAA CCAGGAAACA AAATATGATT 
CTCTCTGATG AAGCAATTAA ATATAAGGAT AAAATCAAGA CACTTGAAAA AAATCAGGAA 
ATTCTGGATG ACACAGCTAA AAATCTTCGT GTTATGCTAG AATCTGAGAG AGAACAGAAT 
GTCAAGAATC AGGACTTGTT GCAGCAGGAA ATCGAAGACT GGAGTAAATT ACATGCTGAG 
CTCAGTGAGC AAATCAAATC ATTTGAGAAG TCTCAGAAAG ATTTGGAAGT AGCTCTTACT 
CACAAGGATG ATAATATTAA TGCTTTGACT AACTGCATTA CACAGTTGAA TCTGTTAGAG 
TGTGAATCTG AATCTGAGGG TCAAAATAAA GGTGGAAATG ATTCAGATGA ATTAGCAAAT 
GGAGAAGTGG GAGGTGACCG GAATGAGAAG ATGAAAAATC AAATTAAGCA GATGATGGAT 
GTCTCTCGGA CACAGACTGC AATATCGGTA GTTGAAGAGG ATCTAAAGCT TTTACAGCTT 
AAGCTAAGAG CCTCCGTGTC CACTAAATGT AACCTGGAAG ACCAGGTAAA GAAATTGGAA 
GATGACCGCA ACTCACTACA AGCTGCCAAA GCTGGACTGG AAGATGAATG CAAAACCTTG 
AGGCAGAAAG TGGAGATTCT GAATGAGCTC TATCAGCAGA AGGAGATGGC TTTGCAAAAG 
AAACTGAGTC AAGAAGAGTA TGAACGGCAA GAAAGAGAGC ACAGGCTGTC AGCTGCAGAT 
GAAAAGGCAG TTTCGGCTGC AGAGGAAGTA AAAACTTACA AGCGGAGAAT TGAAGAAATG 
GAGGATGAAT TACAGAAGAC AGAGCGGTCA TTTAAAAACC AGATCGCTAC CCATGAGAAG 
AAAGCTCATG AAAACTGGCT CAAAGCTCGT GCTGCAGAAA GAGCTATAGC TGAAGAGAAA 
AGGGAAGCTG CCAATTTGAG ACACAAATTA TTAGAATTAA CACAAAAGAT GGCAATGCTG 
CAAGAAGAAC CTGTGATTGT AAAACCAATG CCAGGAAAAC CAAATACACA AAACCCTCCA 
CGGAGAGGTC CTCTGAGCCA GAATGGCTCT TTTGGCCCAT CCCCTGTGAG TGGTGGAGAA 
TGCTCCCCTC CATTGACAGT GGAGCCACCC GTGAGACCTC TCTCTGCTAC TCTCAATCGA 
AGAGATATGC CTAGAAGTGA ATTTGGATCA GTGGACGGGC CTCTACCTCA TCCTCGATGG 
TCAGCTGAGG CATCTGGGAA ACCCTCTCCT TCTGATCCAG GATCTGGTAC AGCTACCATG 
ATGAACAGCA GCTCAAGAGG CTCTTCCCCT ACCAGGGTAC TCGATGAAGG CAAGCAAACT 
GTTCTCCAAG AGCCTGAAGT CCCCTCAGTT CCCAGCATTA CATCTTTGGC TGAGCGTCCA 
GTAGCAGTTA ATATGGCTCC AAAAGGGCCC CCTCCTTTCC CAGGAGTCCC TCTCATGAGC 
ACCCCCATGG GAGGCCCTGT ACCACCACCC ATTCGATATG GACCACCACC TCAGCTCTGC 
GGACCTTTTG GGCCTCGGCC ACTTCCTCCA CCCTTTGGCC CTGGTATGCG TCCACCACTA 
GGCTTAAGAG AATTTGCACC AGGCGTTCCA CCAGGAAGAC GGGACCTGCC TCTCCACCCT 
CGGGGATTTT TACCTGGACA CGCACCATTT AGACCTTTAG GTTCACTTGG CCCAAGAGAG 
TACTTTATTC CTGGTACCCG ATTACCACCC CCAACCCATG GTCCCCAGGA ATACCCACCA 
CCACCTGCTG TAAGAGACTT ACTGCCGTCA GGCTCTAGAG ATGAGCCTCC ACCTGCCTCT 
CAGAGCACTA GCCAGGACTG TTCACAGGCT TTAAAACAGA GCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_011509513.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011507815.2 Homo sapiens melanoma inhibitory activity family member 3 (MIA3), transcript variant X3, mRNA.

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CloneID	OHu87372
Clone ID Related Accession (Same CDS sequence)	XM_011509513.2
Accession Version	XM_011509513.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4911bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	melanoma inhibitory activity protein 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509513.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCCCGG CGCTCCGCCT GGTCCCTGCC CGCGTCCCTG CCCCCGTCCC TGCCCCCGTC 
CCTGCCCCCG TCCCCGCCCC GTCCCCGCCC CCGCCCCCGC CCCCGTCCCC GCCCCCGTCC 
CCGCCCCGTC CCCGCCCCAG CCCCCGCCCC AGCCCCCCCG TCCCCGCCCC GTCCCCGCCC 
CCGTCCCGCC CCTCTGCGCC CGCCCCGGCC TCCTCCTCCG CGTCACCGGC TCCCCGAGGT 
GACCACAACA TGGCTGCGGC GCCTGGGCTG CTCGTCTGGC TGCTCGTGCT CCGGCTGCCC 
TGGCGGGTGC CGGGCCAGCT GGACCCCAGC ACTGGCCGGC GGTTCTCGGA GCACAAACTC 
TGCGCGGACG ACGAATGCAG CATGTTAATG TACCGCGGTG AGGCTCTTGA AGATTTCACA 
GGCCCGGATT GTCGTTTTGT GAATTTTAAA AAAGGTGATC CTGTATATGT TTACTATAAA 
CTGGCAAGAG GATGGCCTGA AGTTTGGGCT GGAAGTGTTG GACGCACTTT TGGATATTTT 
CCAAAAGATT TAATCCAGGT AGTTCATGAA TATACCAAAG AAGAGCTACA AGTTCCAACA 
GATGAGACGG ATTTTGTTTG TTTTGATGGA GGAAGAGATG ATTTTCATAA TTATAATGTA 
GAAGAACTTT TAGGGTTTTT GGAACTGTAC AATTCTGCAG CTACAGATTC TGAGAAAGCT 
GTAGAAAAAA CTTTACAGGA TATGGAAAAA AACCCTGAAT TATCTAAGGA AAGGGAACCT 
GAACCTGAAC CAGTAGAAGC CAACTCAGAG GAAAGTGATA GTGTATTCTC AGAAAACACT 
GAGGATCTTC AGGAACAGTT TACAACTCAG AAGCACCACT CCCATGCAAA CAGCCAAGCA 
AATCATGCTC AGGGAGAGCA GGCTTCATTT GAATCTTTTG AAGAAATGCT GCAAGATAAA 
CTAAAAGTGC CAGAAAGTGA AAACAACAAA ACCAGCAATA GTTCTCAGGT CTCAAATGAA 
CAGGATAAGA TTGATGCCTA TAAACTTTTG AAAAAAGAAA TGACTCTAGA CTTGAAAACC 
AAATTTGGCT CAACAGCTGA TGCACTTGTA TCTGATGATG AGACAACCAG ACTCGTTACT 
TCATTAGAAG ATGATTTTGA TGAGGAATTG GATACTGAGT ATTATGCAGT TGGAAAGGAA 
GATGAGGAGA ACCAAGAAGA CTTTGATGAG TTGCCATTAC TTACCTTTAC AGATGGGGAA 
GATATGAAAA CTCCAGCAAA GTCTGGCGTT GAGAAATATC CAACAGATAA AGAGCAGAAT 
TCAAATGAAG AGGACAAGGT TCAGCTAACT GTGCCCCCTG GCATCAAAAA TGATGATAAA 
AATATACTAA CAACCTGGGG GGACACTATC TTCTCTATTG TCACAGGAGG TGAAGAAACA 
AGAGATACGA TGGATTTAGA GAGCTCTAGT TCAGAGGAAG AAAAAGAAGA TGATGATGAT 
GCATTAGTCC CAGATAGCAA ACAGGGGAAA CCACAGTCAG CAACAGATTA TAGTGACCCT 
GACAATGTAG ATGATGGTCT TTTTATTGTA GACATTCCTA AAACAAATAA TGACAAAGAA 
GTAAACGCAG AACATCACAT TAAAGGAAAA GGGAGGGGAG TTCAGGAATC CAAGAGGGGC 
CTGGTACAAG ATAAGACAGA ATTAGAGGAT GAAAATCAAG AAGGCATGAC TGTGCACAGT 
TCTGTTCACA GCAATAACCT CAACTCTATG CCAGCTGCTG AAAAGGGTAA AGACACATTA 
AAATCAGCTT ATGATGATAC AGAAAATGAC CTAAAAGGAG CAGCTATTCA TATCTCAAAA 
GGAATGCTCC ACGAAGAAAA GCCTGGAGAG CAGATTTTGG AAGGTGGCTC AGAGAGTGAA 
TCTGCACAGA AAGCTGCAGG GAATCAAATG AATGACAGAA AGATTCAACA GGAATCCCTG 
GGTAGTGCAC CACTCATGGG AGATGACCAC CCTAACGCAT CCAGAGACAG TGTGGAGGGA 
GACGCTTTGG TAAATGGGGC CAAACTGCAC ACGCTTTCAG TGGAGCATCA ACGTGAGGAA 
TTGAAAGAGG AATTAGTTCT TAAAACTCAA AACCAACCTA GATTCTCCTC TCCAGATGAG 
ATTGATTTGC CCAGAGAACT GGAAGACGAG GTTCCCATTC TGGGAAGAAA TCTTCCCTGG 
CAACAAGAAA GAGATGTGGC TGCCACAGCC AGTAAGCAAA TGAGTGAGAA GATAAGGCTC 
TCTGAGGGAG AAGCCAAAGA GGACTCCTTG GATGAAGAGT TTTTTCATCA CAAGGCAATG 
CAGGGCACAG AGGTAGGACA GACAGACCAA ACTGACAGCA CAGGAGGACC AGCTTTCCTT 
TCTAAAGTAG AAGAGGATGA TTATCCCTCT GAAGAACTAC TAGAGGATGA AAACGCTATA 
AATGCAAAAC GGTCTAAAGA AAAAAACCCT GGGAATCAGG GCAGGCAGTT TGATGTTAAT 
CTGCAAGTCC CTGACAGAGC AGTTTTAGGG ACCATTCATC CAGATCCAGA AATTGAAGAA 
AGCAAGCAAG AAACTAGTAT GATTTTGGAT AGCGAAAAAA CAAGTGAGAC TGCTGCCAAA 
GGGGTCAACA CAGGAGGCAG GGAACCAAAT ACAATGGTGG AAAAAGAACG CCCTCTGGCA 
GATAAGAAAG CACAGAGACC ATTTGAACGA AGTGACTTTT CTGACAGCAT AAAAATTCAG 
ACTCCAGAAT TAGGTGAAGT GTTTCAGAAT AAAGATTCTG ATTATCTGAA GAACGACAAC 
CCTGAGGAAC ATCTGAAGAC CTCAGGGCTT GCAGGGGAGC CTGAGGGAGA ACTCTCAAAA 
GAGGACCATG AGAACACAGA GAAGTACATG GGCACAGAAA GCCAGGGGTC TGCTGCTGCA 
GAACCTGAAG ATGACTCGTT CCACTGGACT CCACATACAA GTGTAGAGCC AGGGCATAGT 
GACAAGAGGG AGGACTTACT TATCATAAGC AGCTTCTTTA AAGAACAACA GTCTTTGCAG 
CGGTTCCAGA AGTACTTTAA TGTCCATGAG CTGGAAGCCT TGCTACAAGA AATGTCATCA 
AAACTGAAGT CAGCGCAGCA GGAGAGCCTG CCCTATAATA TGGAAAAAGT CCTAGATAAG 
GTCTTCCGTG CTTCTGAGTC ACAAATTCTG AGCATAGCAG AAAAAATGCT TGATACTCGT 
GTGGCTGAAA ATAGAGATCT GGGAATGAAC GAAAATAACA TATTTGAAGA GGCTGCAGTG 
CTTGATGACA TTCAAGACCT CATCTATTTT GTCAGGTACA AGCACTCCAC AGCAGAGGAG 
ACAGCCACAC TGGTGATGGC ACCACCTCTA GAGGAAGGCT TGGGTGGAGC AATGGAAGAG 
ATGCAACCAC TGCATGAAGA TAATTTCTCA CGAGAGAAGA CAGCAGAACT TAATGTGCAG 
GTTCCTGAAG AACCCACCCA CTTGGACCAA CGTGTGATTG GGGACACTCA TGCCTCAGAA 
GTGTCACAGA AGCCAAATAC TGAGAAAGAC CTGGACCCAG GGCCAGTTAC AACAGAAGAC 
ACTCCTATGG ATGCTATTGA TGCAAACAAG CAACCAGAGA CAGCCGCCGA AGAGCCGGCA 
AGTGTCACAC CTTTGGAAAA CGCAATCCTT CTAATATATT CATTCATGTT TTATTTAACT 
AAGTCGCTAG TTGCTACATT GCCTGATGAT GTTCAGCCTG GGCCTGATTT TTATGGACTG 
CCATGGAAAC CTGTATTTAT CACTGCCTTC TTGGGAATTG CTTCGTTTGC CATTTTCTTA 
TGGAGAACTG TCCTTGTTGT GAAGGATAGA GTATATCAAG TCACGGAACA GCAAATTTCT 
GAGAAGTTGA AGACTATCAT GAAAGAAAAT ACAGAACTTG TACAAAAATT GTCAAATTAT 
GAACAGAAGA TCAAGGAATC AAAGAAACAT GTTCAGGAAA CCAGGAAACA AAATATGATT 
CTCTCTGATG AAGCAATTAA ATATAAGGAT AAAATCAAGA CACTTGAAAA AAATCAGGAA 
ATTCTGGATG ACACAGCTAA AAATCTTCGT GTTATGCTAG AATCTGAGAG AGAACAGAAT 
GTCAAGAATC AGGACTTGAT ATCAGAAAAC AAGAAATCTA TAGAGAAGTT AAAGGATGTT 
ATTTCAATGA ATGCCTCAGA ATTTTCAGAG GTTCAGATTG CACTTAATGA AGCTAAGCTT 
AGTGAAGAGA AGGTGAAGTC TGAATGCCAT CGGGTTCAAG AAGAAAATGC TAGGCTTAAG 
AAGAAAAAAG AGCAGTTGCA GCAGGAAATC GAAGACTGGA GTAAATTACA TGCTGAGCTC 
AGTGAGCAAA TCAAATCATT TGAGAAGTCT CAGAAAGATT TGGAAGTAGC TCTTACTCAC 
AAGGATGATA ATATTAATGC TTTGACTAAC TGCATTACAC AGTTGAATCT GTTAGAGTGT 
GAATCTGAAT CTGAGGGTCA AAATAAAGGT GGAAATGATT CAGATGAATT AGCAAATGGA 
GAAGTGGGAG GTGACCGGAA TGAGAAGATG AAAAATCAAA TTAAGCAGAT GATGGATGTC 
TCTCGGACAC AGACTGCAAT ATCGGTAGTT GAAGAGGATC TAAAGCTTTT ACAGCTTAAG 
CTAAGAGCCT CCGTGTCCAC TAAATGTAAC CTGGAAGACC AGGTAAAGAA ATTGGAAGAT 
GACCGCAACT CACTACAAGC TGCCAAAGCT GGACTGGAAG ATGAATGCAA AACCTTGAGG 
CAGAAAGTGG AGATTCTGAA TGAGCTCTAT CAGCAGAAGG AGATGGCTTT GCAAAAAAAA 
TTTGCAAGTA TCTTTTGCAT CAGAACTATG AGTAAAGAAG TACAAGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011507815.2
CDS	73..4983
Translation	MPPALRLVPARVPAPVPAPVPAPVPAPSPPPPPPPSPPPSPPRPRPSPRPSPPVPAPSPPPSRPSAPAPASSSASPAPRGDHNMAAAPGLLVWLLVLRLPWRVPGQLDPSTGRRFSEHKLCADDECSMLMYRGEALEDFTGPDCRFVNFKKGDPVYVYYKLARGWPEVWAGSVGRTFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFHNYNVEELLGFLELYNSAATDSEKAVEKTLQDMEKNPELSKEREPEPEPVEANSEESDSVFSENTEDLQEQFTTQKHHSHANSQANHAQGEQASFESFEEMLQDKLKVPESENNKTSNSSQVSNEQDKIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDTEYYAVGKEDEENQEDFDELPLLTFTDGEDMKTPAKSGVEKYPTDKEQNSNEEDKVQLTVPPGIKNDDKNILTTWGDTIFSIVTGGEETRDTMDLESSSSEEEKEDDDDALVPDSKQGKPQSATDYSDPDNVDDGLFIVDIPKTNNDKEVNAEHHIKGKGRGVQESKRGLVQDKTELEDENQEGMTVHSSVHSNNLNSMPAAEKGKDTLKSAYDDTENDLKGAAIHISKGMLHEEKPGEQILEGGSESESAQKAAGNQMNDRKIQQESLGSAPLMGDDHPNASRDSVEGDALVNGAKLHTLSVEHQREELKEELVLKTQNQPRFSSPDEIDLPRELEDEVPILGRNLPWQQERDVAATASKQMSEKIRLSEGEAKEDSLDEEFFHHKAMQGTEVGQTDQTDSTGGPAFLSKVEEDDYPSEELLEDENAINAKRSKEKNPGNQGRQFDVNLQVPDRAVLGTIHPDPEIEESKQETSMILDSEKTSETAAKGVNTGGREPNTMVEKERPLADKKAQRPFERSDFSDSIKIQTPELGEVFQNKDSDYLKNDNPEEHLKTSGLAGEPEGELSKEDHENTEKYMGTESQGSAAAEPEDDSFHWTPHTSVEPGHSDKREDLLIISSFFKEQQSLQRFQKYFNVHELEALLQEMSSKLKSAQQESLPYNMEKVLDKVFRASESQILSIAEKMLDTRVAENRDLGMNENNIFEEAAVLDDIQDLIYFVRYKHSTAEETATLVMAPPLEEGLGGAMEEMQPLHEDNFSREKTAELNVQVPEEPTHLDQRVIGDTHASEVSQKPNTEKDLDPGPVTTEDTPMDAIDANKQPETAAEEPASVTPLENAILLIYSFMFYLTKSLVATLPDDVQPGPDFYGLPWKPVFITAFLGIASFAIFLWRTVLVVKDRVYQVTEQQISEKLKTIMKENTELVQKLSNYEQKIKESKKHVQETRKQNMILSDEAIKYKDKIKTLEKNQEILDDTAKNLRVMLESEREQNVKNQDLISENKKSIEKLKDVISMNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIEDWSKLHAELSEQIKSFEKSQKDLEVALTHKDDNINALTNCITQLNLLECESESEGQNKGGNDSDELANGEVGGDRNEKMKNQIKQMMDVSRTQTAISVVEEDLKLLQLKLRASVSTKCNLEDQVKKLEDDRNSLQAAKAGLEDECKTLRQKVEILNELYQQKEMALQKKFASIFCIRTMSKEVQG

Target ORF information:

RefSeq Version	XM_011509513.2
Organism	Homo sapiens(human)
Definition	Homo sapiens melanoma inhibitory activity family member 3 (MIA3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509513.2

ORF Insert Sequence:

ATGCCCCCGG CGCTCCGCCT GGTCCCTGCC CGCGTCCCTG CCCCCGTCCC TGCCCCCGTC 
CCTGCCCCCG TCCCCGCCCC GTCCCCGCCC CCGCCCCCGC CCCCGTCCCC GCCCCCGTCC 
CCGCCCCGTC CCCGCCCCAG CCCCCGCCCC AGCCCCCCCG TCCCCGCCCC GTCCCCGCCC 
CCGTCCCGCC CCTCTGCGCC CGCCCCGGCC TCCTCCTCCG CGTCACCGGC TCCCCGAGGT 
GACCACAACA TGGCTGCGGC GCCTGGGCTG CTCGTCTGGC TGCTCGTGCT CCGGCTGCCC 
TGGCGGGTGC CGGGCCAGCT GGACCCCAGC ACTGGCCGGC GGTTCTCGGA GCACAAACTC 
TGCGCGGACG ACGAATGCAG CATGTTAATG TACCGCGGTG AGGCTCTTGA AGATTTCACA 
GGCCCGGATT GTCGTTTTGT GAATTTTAAA AAAGGTGATC CTGTATATGT TTACTATAAA 
CTGGCAAGAG GATGGCCTGA AGTTTGGGCT GGAAGTGTTG GACGCACTTT TGGATATTTT 
CCAAAAGATT TAATCCAGGT AGTTCATGAA TATACCAAAG AAGAGCTACA AGTTCCAACA 
GATGAGACGG ATTTTGTTTG TTTTGATGGA GGAAGAGATG ATTTTCATAA TTATAATGTA 
GAAGAACTTT TAGGGTTTTT GGAACTGTAC AATTCTGCAG CTACAGATTC TGAGAAAGCT 
GTAGAAAAAA CTTTACAGGA TATGGAAAAA AACCCTGAAT TATCTAAGGA AAGGGAACCT 
GAACCTGAAC CAGTAGAAGC CAACTCAGAG GAAAGTGATA GTGTATTCTC AGAAAACACT 
GAGGATCTTC AGGAACAGTT TACAACTCAG AAGCACCACT CCCATGCAAA CAGCCAAGCA 
AATCATGCTC AGGGAGAGCA GGCTTCATTT GAATCTTTTG AAGAAATGCT GCAAGATAAA 
CTAAAAGTGC CAGAAAGTGA AAACAACAAA ACCAGCAATA GTTCTCAGGT CTCAAATGAA 
CAGGATAAGA TTGATGCCTA TAAACTTTTG AAAAAAGAAA TGACTCTAGA CTTGAAAACC 
AAATTTGGCT CAACAGCTGA TGCACTTGTA TCTGATGATG AGACAACCAG ACTCGTTACT 
TCATTAGAAG ATGATTTTGA TGAGGAATTG GATACTGAGT ATTATGCAGT TGGAAAGGAA 
GATGAGGAGA ACCAAGAAGA CTTTGATGAG TTGCCATTAC TTACCTTTAC AGATGGGGAA 
GATATGAAAA CTCCAGCAAA GTCTGGCGTT GAGAAATATC CAACAGATAA AGAGCAGAAT 
TCAAATGAAG AGGACAAGGT TCAGCTAACT GTGCCCCCTG GCATCAAAAA TGATGATAAA 
AATATACTAA CAACCTGGGG GGACACTATC TTCTCTATTG TCACAGGAGG TGAAGAAACA 
AGAGATACGA TGGATTTAGA GAGCTCTAGT TCAGAGGAAG AAAAAGAAGA TGATGATGAT 
GCATTAGTCC CAGATAGCAA ACAGGGGAAA CCACAGTCAG CAACAGATTA TAGTGACCCT 
GACAATGTAG ATGATGGTCT TTTTATTGTA GACATTCCTA AAACAAATAA TGACAAAGAA 
GTAAACGCAG AACATCACAT TAAAGGAAAA GGGAGGGGAG TTCAGGAATC CAAGAGGGGC 
CTGGTACAAG ATAAGACAGA ATTAGAGGAT GAAAATCAAG AAGGCATGAC TGTGCACAGT 
TCTGTTCACA GCAATAACCT CAACTCTATG CCAGCTGCTG AAAAGGGTAA AGACACATTA 
AAATCAGCTT ATGATGATAC AGAAAATGAC CTAAAAGGAG CAGCTATTCA TATCTCAAAA 
GGAATGCTCC ACGAAGAAAA GCCTGGAGAG CAGATTTTGG AAGGTGGCTC AGAGAGTGAA 
TCTGCACAGA AAGCTGCAGG GAATCAAATG AATGACAGAA AGATTCAACA GGAATCCCTG 
GGTAGTGCAC CACTCATGGG AGATGACCAC CCTAACGCAT CCAGAGACAG TGTGGAGGGA 
GACGCTTTGG TAAATGGGGC CAAACTGCAC ACGCTTTCAG TGGAGCATCA ACGTGAGGAA 
TTGAAAGAGG AATTAGTTCT TAAAACTCAA AACCAACCTA GATTCTCCTC TCCAGATGAG 
ATTGATTTGC CCAGAGAACT GGAAGACGAG GTTCCCATTC TGGGAAGAAA TCTTCCCTGG 
CAACAAGAAA GAGATGTGGC TGCCACAGCC AGTAAGCAAA TGAGTGAGAA GATAAGGCTC 
TCTGAGGGAG AAGCCAAAGA GGACTCCTTG GATGAAGAGT TTTTTCATCA CAAGGCAATG 
CAGGGCACAG AGGTAGGACA GACAGACCAA ACTGACAGCA CAGGAGGACC AGCTTTCCTT 
TCTAAAGTAG AAGAGGATGA TTATCCCTCT GAAGAACTAC TAGAGGATGA AAACGCTATA 
AATGCAAAAC GGTCTAAAGA AAAAAACCCT GGGAATCAGG GCAGGCAGTT TGATGTTAAT 
CTGCAAGTCC CTGACAGAGC AGTTTTAGGG ACCATTCATC CAGATCCAGA AATTGAAGAA 
AGCAAGCAAG AAACTAGTAT GATTTTGGAT AGCGAAAAAA CAAGTGAGAC TGCTGCCAAA 
GGGGTCAACA CAGGAGGCAG GGAACCAAAT ACAATGGTGG AAAAAGAACG CCCTCTGGCA 
GATAAGAAAG CACAGAGACC ATTTGAACGA AGTGACTTTT CTGACAGCAT AAAAATTCAG 
ACTCCAGAAT TAGGTGAAGT GTTTCAGAAT AAAGATTCTG ATTATCTGAA GAACGACAAC 
CCTGAGGAAC ATCTGAAGAC CTCAGGGCTT GCAGGGGAGC CTGAGGGAGA ACTCTCAAAA 
GAGGACCATG AGAACACAGA GAAGTACATG GGCACAGAAA GCCAGGGGTC TGCTGCTGCA 
GAACCTGAAG ATGACTCGTT CCACTGGACT CCACATACAA GTGTAGAGCC AGGGCATAGT 
GACAAGAGGG AGGACTTACT TATCATAAGC AGCTTCTTTA AAGAACAACA GTCTTTGCAG 
CGGTTCCAGA AGTACTTTAA TGTCCATGAG CTGGAAGCCT TGCTACAAGA AATGTCATCA 
AAACTGAAGT CAGCGCAGCA GGAGAGCCTG CCCTATAATA TGGAAAAAGT CCTAGATAAG 
GTCTTCCGTG CTTCTGAGTC ACAAATTCTG AGCATAGCAG AAAAAATGCT TGATACTCGT 
GTGGCTGAAA ATAGAGATCT GGGAATGAAC GAAAATAACA TATTTGAAGA GGCTGCAGTG 
CTTGATGACA TTCAAGACCT CATCTATTTT GTCAGGTACA AGCACTCCAC AGCAGAGGAG 
ACAGCCACAC TGGTGATGGC ACCACCTCTA GAGGAAGGCT TGGGTGGAGC AATGGAAGAG 
ATGCAACCAC TGCATGAAGA TAATTTCTCA CGAGAGAAGA CAGCAGAACT TAATGTGCAG 
GTTCCTGAAG AACCCACCCA CTTGGACCAA CGTGTGATTG GGGACACTCA TGCCTCAGAA 
GTGTCACAGA AGCCAAATAC TGAGAAAGAC CTGGACCCAG GGCCAGTTAC AACAGAAGAC 
ACTCCTATGG ATGCTATTGA TGCAAACAAG CAACCAGAGA CAGCCGCCGA AGAGCCGGCA 
AGTGTCACAC CTTTGGAAAA CGCAATCCTT CTAATATATT CATTCATGTT TTATTTAACT 
AAGTCGCTAG TTGCTACATT GCCTGATGAT GTTCAGCCTG GGCCTGATTT TTATGGACTG 
CCATGGAAAC CTGTATTTAT CACTGCCTTC TTGGGAATTG CTTCGTTTGC CATTTTCTTA 
TGGAGAACTG TCCTTGTTGT GAAGGATAGA GTATATCAAG TCACGGAACA GCAAATTTCT 
GAGAAGTTGA AGACTATCAT GAAAGAAAAT ACAGAACTTG TACAAAAATT GTCAAATTAT 
GAACAGAAGA TCAAGGAATC AAAGAAACAT GTTCAGGAAA CCAGGAAACA AAATATGATT 
CTCTCTGATG AAGCAATTAA ATATAAGGAT AAAATCAAGA CACTTGAAAA AAATCAGGAA 
ATTCTGGATG ACACAGCTAA AAATCTTCGT GTTATGCTAG AATCTGAGAG AGAACAGAAT 
GTCAAGAATC AGGACTTGAT ATCAGAAAAC AAGAAATCTA TAGAGAAGTT AAAGGATGTT 
ATTTCAATGA ATGCCTCAGA ATTTTCAGAG GTTCAGATTG CACTTAATGA AGCTAAGCTT 
AGTGAAGAGA AGGTGAAGTC TGAATGCCAT CGGGTTCAAG AAGAAAATGC TAGGCTTAAG 
AAGAAAAAAG AGCAGTTGCA GCAGGAAATC GAAGACTGGA GTAAATTACA TGCTGAGCTC 
AGTGAGCAAA TCAAATCATT TGAGAAGTCT CAGAAAGATT TGGAAGTAGC TCTTACTCAC 
AAGGATGATA ATATTAATGC TTTGACTAAC TGCATTACAC AGTTGAATCT GTTAGAGTGT 
GAATCTGAAT CTGAGGGTCA AAATAAAGGT GGAAATGATT CAGATGAATT AGCAAATGGA 
GAAGTGGGAG GTGACCGGAA TGAGAAGATG AAAAATCAAA TTAAGCAGAT GATGGATGTC 
TCTCGGACAC AGACTGCAAT ATCGGTAGTT GAAGAGGATC TAAAGCTTTT ACAGCTTAAG 
CTAAGAGCCT CCGTGTCCAC TAAATGTAAC CTGGAAGACC AGGTAAAGAA ATTGGAAGAT 
GACCGCAACT CACTACAAGC TGCCAAAGCT GGACTGGAAG ATGAATGCAA AACCTTGAGG 
CAGAAAGTGG AGATTCTGAA TGAGCTCTAT CAGCAGAAGG AGATGGCTTT GCAAAAAAAA 
TTTGCAAGTA TCTTTTGCAT CAGAACTATG AGTAAAGAAG TACAAGGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MIA3
Entrez Gene ID	375056
Full Name	MIA family member 3, ER export factor
Synonyms	ARNT,D320,TANGO,TANGO1,UNQ6077
General protein information	Preferred Names MIA family member 3, ER export factor Names transport and Golgi organization protein 1 homolog melanoma inhibitory activity protein 3 C219-reactive peptide melanoma inhibitory activity family, member 3 transport and Golgi organization protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q41
Disorder MIM:	613455

Mus musculus (house mouse)	Mia3	NP_796363.2
Rattus norvegicus (Norway rat)	Mia3	XP_006250418.1
	MIA3	XP_001100730.1
Bos taurus (cattle)	MIA3	NP_001160043.1
Homo sapiens (human)	MIA3	NP_940953.2
Canis lupus familiaris (dog)	MIA3	XP_005640892.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MIA3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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