MIA3 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MIA3
Entrez Gene ID	509133
Full Name	MIA family member 3, ER export factor
General protein information	Preferred Names MIA family member 3, ER export factor Names transport and Golgi organization protein 1 homolog TANGO1 melanoma inhibitory activity family, member 3 melanoma inhibitory activity protein 3 transport and Golgi organization protein 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024976152.1	XP_024831920.1	transport and Golgi organization protein 1 homolog isoform X1
XM_024976156.1	XP_024831924.1	transport and Golgi organization protein 1 homolog isoform X5
XM_024976153.1	XP_024831921.1	transport and Golgi organization protein 1 homolog isoform X2
XM_024976155.1	XP_024831923.1	transport and Golgi organization protein 1 homolog isoform X4
XM_024976154.1	XP_024831922.1	transport and Golgi organization protein 1 homolog isoform X3
XM_024976157.1	XP_024831925.1	transport and Golgi organization protein 1 homolog isoform X6
NM_001166571.2	NP_001160043.2	transport and Golgi organization protein 1 homolog precursor
NM_001166571.1	NP_001160043.1	melanoma inhibitory activity protein 3 precursor

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mia3	NP_796363.2
Rattus norvegicus (Norway rat)	Mia3	XP_006250418.1
	MIA3	XP_001100730.1
Bos taurus (cattle)	MIA3	NP_001160043.1
Homo sapiens (human)	MIA3	NP_940953.2
Canis lupus familiaris (dog)	MIA3	XP_005640892.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MIA3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MIA3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa223513	XM_024976156.1 Latest version!	Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa223514	XM_024976157.1 Latest version!	Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OBa239258	NM_001166571.2 Latest version!	Bos taurus MIA SH3 domain ER export factor 3 (MIA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223509	XM_024976152.1 Latest version!	Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223510	XM_024976153.1 Latest version!	Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223511	XM_024976154.1 Latest version!	Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa223512	XM_024976155.1 Latest version!	Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa44547	NM_001166571.1 Latest version!	Bos taurus melanoma inhibitory activity family, member 3 (MIA3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MIA3 ( XM_024976156.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831924.1 Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa223513
Clone ID Related Accession (Same CDS sequence)	XM_024976156.1
Accession Version	XM_024976156.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2433bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transport and Golgi organization protein 1 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAG TACCTGCCAC TGTGCCTTCT GTTACCTCTT CCTCGGGGGA CCCGGAGCTT 
CTGGGACCCC TATCGGGGCT CTACGCAGCA CTCATAGCCA GGCTCCTGGA GCTCCTTGCT 
ACATTGCCTG ATGATGTCCA GCCTGGGCCT GATTTTTATG GACTGCCATG GAAGCCTGTA 
CTTATCACTG CCTCCTTAGG AATTGTTTCA TTTGCCGTTT TCTTCTGGAG AACTGTCCTT 
GCTGTAAAGA GTAGAGTATA TCAAGTCACT GAACAGCAAA TTTCTGAGAA GTTGAAGAAT 
ATCATGAAAG AAAACGCAGA ACTTGTACAA AAATTGTCAA GTTATGAGCA GAAGATCAAG 
GAATCAAAGA AACATGTTCA AGAAACCAAG AAACAAAACA TGATTCTTTC TGATGAAGCA 
ATTAAATTTA AGGATAAAAT CAAGAGTCTT GAAGAAACTA ATGAAATTCT GGGAGACACA 
GCTAAAAGTT TACGCGCTAT GTTAGAGTCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC 
TTGATATCAG AAAATAAGAA ATCTATAGAG AAGTTAAAGG ATGTTATCTC AGTGAATGCC 
TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAAGTG 
AAGTCTGAAT GCCATCGGGT TCAAGAAGAA AATGCTAGAC TTAAGAAGAA GAAAGAGCAG 
TTGCAGCAAG AAATCAAAGA CTGGAGTAAA TCACATGCTG AGCTCAGTGA GCAAATCAGA 
TCATTTGAGA AGTCTCAGAA AGATCTAGAA GTAGCTCTCA CTCACAAAGA TGATAATATT 
AATGCTTTGA CTAATTGCAT TACACAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG 
GATCAAAATA AAGGAGGAAG TGAATCAGAT GAATTAGCAA ATGGAGAAGT GGGAGGTGAC 
CGGAGTGAGA AGGTGAAAAA TCAGATTAAG CAGATGATGG ACGTTTCTCG GACACAGACT 
GCAATATCAG TAGTTGAAGA GGATCTGAAA CTTTTACAGT GTAAACTCAG AGCCTCCATG 
TCCACTAAGT GTAACCTGGA AGACCAAATA AAGAAATTAG AAGAGGATCG CAGCTCACTT 
CAGTCTGCCA AAACTGTGCT GGAAGATGAA TGCAAAACAC TGAGGCAGAA AGTAGAGATT 
CTAAATGAAC TCTATCAGCA GAAGGAGATG GCTCTACAAA AGAAATTGAG TCAAGAAGAG 
TATGAGCGGC AAGAAAGAGA GCAGCGGCTG TCAGCTGCAG ATGAAAAGGC GGTTTTGGCG 
GCAGAGGAAG TGAAAACTTA CAAGCGGAGA ATTGAAGAAA TGGAGGATGA ACTACAGAAA 
ACAGAGCGCT CATTTAAAAA CCAGATTGCT ACTCATGAGA AGAAAGCTCA CGATAACTGG 
CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCTGAAGAGA AGAGAGAAGC TGCCAACTTG 
AGACATAAAT TATTGGAACT AACCCAAAAG ATGGCAATGA TGCAAGAAGA ACCTGTGATT 
GTAAAACCAA TGCCAGGAAG ACCCAACACA CAAAACCCTC CACGGAGAGG TCCCCTGAGC 
CAGAATGGCT CTTTTGGCCC ATCTCCTGTG AGCGGTGGAG AATGCTCCCC ACCGCTGACG 
GCAGACCCGC CCGCAAGACC TCTGTCTGCT ACGCTTAATC GAAGAGAGAT GCCTCGAAGT 
GAATTTGGAT CAGTGGATGG GCCTCTCCCT CGACCTCGAT GGGCCTCTGA GGCATCTGGG 
AAACCCTCTG CCTCTGATCC AGAATCCGGC GCTGCTCCCA CAGTGAACAG CAGCTCCAGA 
AGCTCTTCGC CCTCTAAGGT GATGGATGAG GGCAAGCAAA CTGTTCCCCA AGAGCCTGAA 
GGCCCTTCAG TTCCCAGCAT TCCTTCTTTG GCTGAGCATC CAGTATCAGT TAGTATGGCT 
GCAAAAGGGC CCCCTCCCTT TCCAGGGACA CCCCTCATGA GCTCCCCTGT GGGAGGCCCT 
CTACTGCCAC CCATCCGATA TGGACCACCT CCTCAACTCT GTGGGCCTTT CGGACCTCGG 
CCACTCCCTC CACCCTTTGG TCCTGGTATG CGTCCACCGC TAGGCTTAAG AGAATATGCA 
CCAGGTGTTC CACCGGGAAA ACGGGACCTG CCTCTCGACC CTCGAGAATT TTTACCACCA 
GGACATGCAC CTTTTAGACC TTTAGGCTCT CTTGGCCCAA GAGAGTACTT TTTTCCTGGT 
ACCCGATTAC CACCCCCCAA TCATGGGCCC CAGGATTATC CACCTTCCTC TGCTGCAAGA 
GACTTACCGC CCTCCGGCTC CAGAGATGAG CCCCCGCCTG CCTCTCAGGG CGCTAGCCAG 
GACTGCTCAC CGGCTTTAAA ACAGAGCCCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831924.1
CDS	159..2591
Translation	MDSVPATVPSVTSSSGDPELLGPLSGLYAALIARLLELLATLPDDVQPGPDFYGLPWKPVLITASLGIVSFAVFFWRTVLAVKSRVYQVTEQQISEKLKNIMKENAELVQKLSSYEQKIKESKKHVQETKKQNMILSDEAIKFKDKIKSLEETNEILGDTAKSLRAMLESEREQNAKNQDLISENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIRSFEKSQKDLEVALTHKDDNINALTNCITQLNRLDCESESEDQNKGGSESDELANGEVGGDRSEKVKNQIKQMMDVSRTQTAISVVEEDLKLLQCKLRASMSTKCNLEDQIKKLEEDRSSLQSAKTVLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKAVLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMMQEEPVIVKPMPGRPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTADPPARPLSATLNRREMPRSEFGSVDGPLPRPRWASEASGKPSASDPESGAAPTVNSSSRSSSPSKVMDEGKQTVPQEPEGPSVPSIPSLAEHPVSVSMAAKGPPPFPGTPLMSSPVGGPLLPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREYAPGVPPGKRDLPLDPREFLPPGHAPFRPLGSLGPREYFFPGTRLPPPNHGPQDYPPSSAARDLPPSGSRDEPPPASQGASQDCSPALKQSP

Target ORF information:

RefSeq Version	XM_024976156.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976156.1

ORF Insert Sequence:

ATGGACTCAG TACCTGCCAC TGTGCCTTCT GTTACCTCTT CCTCGGGGGA CCCGGAGCTT 
CTGGGACCCC TATCGGGGCT CTACGCAGCA CTCATAGCCA GGCTCCTGGA GCTCCTTGCT 
ACATTGCCTG ATGATGTCCA GCCTGGGCCT GATTTTTATG GACTGCCATG GAAGCCTGTA 
CTTATCACTG CCTCCTTAGG AATTGTTTCA TTTGCCGTTT TCTTCTGGAG AACTGTCCTT 
GCTGTAAAGA GTAGAGTATA TCAAGTCACT GAACAGCAAA TTTCTGAGAA GTTGAAGAAT 
ATCATGAAAG AAAACGCAGA ACTTGTACAA AAATTGTCAA GTTATGAGCA GAAGATCAAG 
GAATCAAAGA AACATGTTCA AGAAACCAAG AAACAAAACA TGATTCTTTC TGATGAAGCA 
ATTAAATTTA AGGATAAAAT CAAGAGTCTT GAAGAAACTA ATGAAATTCT GGGAGACACA 
GCTAAAAGTT TACGCGCTAT GTTAGAGTCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC 
TTGATATCAG AAAATAAGAA ATCTATAGAG AAGTTAAAGG ATGTTATCTC AGTGAATGCC 
TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAAGTG 
AAGTCTGAAT GCCATCGGGT TCAAGAAGAA AATGCTAGAC TTAAGAAGAA GAAAGAGCAG 
TTGCAGCAAG AAATCAAAGA CTGGAGTAAA TCACATGCTG AGCTCAGTGA GCAAATCAGA 
TCATTTGAGA AGTCTCAGAA AGATCTAGAA GTAGCTCTCA CTCACAAAGA TGATAATATT 
AATGCTTTGA CTAATTGCAT TACACAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG 
GATCAAAATA AAGGAGGAAG TGAATCAGAT GAATTAGCAA ATGGAGAAGT GGGAGGTGAC 
CGGAGTGAGA AGGTGAAAAA TCAGATTAAG CAGATGATGG ACGTTTCTCG GACACAGACT 
GCAATATCAG TAGTTGAAGA GGATCTGAAA CTTTTACAGT GTAAACTCAG AGCCTCCATG 
TCCACTAAGT GTAACCTGGA AGACCAAATA AAGAAATTAG AAGAGGATCG CAGCTCACTT 
CAGTCTGCCA AAACTGTGCT GGAAGATGAA TGCAAAACAC TGAGGCAGAA AGTAGAGATT 
CTAAATGAAC TCTATCAGCA GAAGGAGATG GCTCTACAAA AGAAATTGAG TCAAGAAGAG 
TATGAGCGGC AAGAAAGAGA GCAGCGGCTG TCAGCTGCAG ATGAAAAGGC GGTTTTGGCG 
GCAGAGGAAG TGAAAACTTA CAAGCGGAGA ATTGAAGAAA TGGAGGATGA ACTACAGAAA 
ACAGAGCGCT CATTTAAAAA CCAGATTGCT ACTCATGAGA AGAAAGCTCA CGATAACTGG 
CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCTGAAGAGA AGAGAGAAGC TGCCAACTTG 
AGACATAAAT TATTGGAACT AACCCAAAAG ATGGCAATGA TGCAAGAAGA ACCTGTGATT 
GTAAAACCAA TGCCAGGAAG ACCCAACACA CAAAACCCTC CACGGAGAGG TCCCCTGAGC 
CAGAATGGCT CTTTTGGCCC ATCTCCTGTG AGCGGTGGAG AATGCTCCCC ACCGCTGACG 
GCAGACCCGC CCGCAAGACC TCTGTCTGCT ACGCTTAATC GAAGAGAGAT GCCTCGAAGT 
GAATTTGGAT CAGTGGATGG GCCTCTCCCT CGACCTCGAT GGGCCTCTGA GGCATCTGGG 
AAACCCTCTG CCTCTGATCC AGAATCCGGC GCTGCTCCCA CAGTGAACAG CAGCTCCAGA 
AGCTCTTCGC CCTCTAAGGT GATGGATGAG GGCAAGCAAA CTGTTCCCCA AGAGCCTGAA 
GGCCCTTCAG TTCCCAGCAT TCCTTCTTTG GCTGAGCATC CAGTATCAGT TAGTATGGCT 
GCAAAAGGGC CCCCTCCCTT TCCAGGGACA CCCCTCATGA GCTCCCCTGT GGGAGGCCCT 
CTACTGCCAC CCATCCGATA TGGACCACCT CCTCAACTCT GTGGGCCTTT CGGACCTCGG 
CCACTCCCTC CACCCTTTGG TCCTGGTATG CGTCCACCGC TAGGCTTAAG AGAATATGCA 
CCAGGTGTTC CACCGGGAAA ACGGGACCTG CCTCTCGACC CTCGAGAATT TTTACCACCA 
GGACATGCAC CTTTTAGACC TTTAGGCTCT CTTGGCCCAA GAGAGTACTT TTTTCCTGGT 
ACCCGATTAC CACCCCCCAA TCATGGGCCC CAGGATTATC CACCTTCCTC TGCTGCAAGA 
GACTTACCGC CCTCCGGCTC CAGAGATGAG CCCCCGCCTG CCTCTCAGGG CGCTAGCCAG 
GACTGCTCAC CGGCTTTAAA ACAGAGCCCA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_024976157.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831925.1 Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa223514
Clone ID Related Accession (Same CDS sequence)	XM_024976157.1
Accession Version	XM_024976157.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2361bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transport and Golgi organization protein 1 homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCAG TACCTGCCAC TGTGCCTTCT GTTACCTCTT CCTCGGGGGA CCCGGAGCTT 
CTGGGACCCC TATCGGGGCT CTACGCAGCA CTCATAGCCA GGCTCCTGGA GCTCCTTGCT 
ACATTGCCTG ATGATGTCCA GCCTGGGCCT GATTTTTATG GACTGCCATG GAAGCCTGTA 
CTTATCACTG CCTCCTTAGG AATTGTTTCA TTTGCCGTTT TCTTCTGGAG AACTGTCCTT 
GCTGTAAAGA GTAGAGTATA TCAAGTCACT GAACAGCAAA TTTCTGAGAA GTTGAAGAAT 
ATCATGAAAG AAAACGCAGA ACTTGTACAA AAATTGTCAA GTTATGAGCA GAAGATCAAG 
GAATCAAAGA AACATGTTCA AGAAACCAAG AAACAAAACA TGATTCTTTC TGATGAAGCA 
ATTAAATTTA AGGATAAAAT CAAGAGTCTT GAAGAAACTA ATGAAATTCT GGGAGACACA 
GCTAAAAGTT TACGCGCTAT GTTAGAGTCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC 
TTGATATCAG AAAATAAGAA ATCTATAGAG AAGTTAAAGG ATGTTATCTC AGTGAATGCC 
TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAAGTG 
AAGTCTGAAT GCCATCGGGT TCAAGAAGAA AATGCTAGAC TTAAGAAGAA GAAAGAGCAG 
TTGCAGCAAG AAATCAAAGA CTGGAGTAAA TCACATGCTG AGCTCAGTGA GCAAATCAGA 
TCATTTGAGA AGTCTCAGAA AGATCTAGAA GTAGCTCTCA CTCACAAAGA TGATAATATT 
AATGCTTTGA CTAATTGCAT TACACAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG 
GATCAAAATA AAGGAGGAAG TGAATCAGAT GAATTAGCAA ATGGAGAAGT GGGAGGTGAC 
CGGAGTGAGA AGGTGAAAAA TCAGATTAAG CAGATGATGG ACGTTTCTCG GACACAGACT 
GCAATATCAG TAGTTGAAGA GGATCTGAAA CTTTTACAGT GTAAACTCAG AGCCTCCATG 
TCCACTAAGT GTAACCTGGA AGACCAAATA AAGAAATTAG AAGAGGATCG CAGCTCACTT 
CAGTCTGCCA AAACTGTGCT GGAAGATGAA TGCAAAACAC TGAGGCAGAA AGTAGAGATT 
CTAAATGAAC TCTATCAGCA GAAGGAGATG GCTCTACAAA AGAAATTGAG TCAAGAAGAG 
TATGAGCGGC AAGAAAGAGA GCAGCGGCTG TCAGCTGCAG ATGAAAAGGC GGTTTTGGCG 
GCAGAGGAAG TGAAAACTTA CAAGCGGAGA ATTGAAGAAA TGGAGGATGA ACTACAGAAA 
ACAGAGCGCT CATTTAAAAA CCAGATTGCT ACTCATGAGA AGAAAGCTCA CGATAACTGG 
CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCTGAAGAGA AGAGAGAAGC TGCCAACTTG 
AGACATAAAT TATTGGAACT AACCCAAAAG ATGGCAATGA TGCAAGAAGA ACCTGTGATT 
GTAAAACCAA TGCCAGGAAG ACCCAACACA CAAAACCCTC CACGGAGAGG TCCCCTGAGC 
CAGAATGGCT CTTTTGGCCC ATCTCCTGTG AGCGGTGGAG AATGCTCCCC ACCGCTGACG 
GCAGACCCGC CCGCAAGACC TCTGTCTGCT ACGCTTAATC GAAGAGAGAT GCCTCGAAGT 
GAATTTGGAT CAGTGGATGG GCCTCTCCCT CGACCTCGAT GGGCCTCTGA GGCATCTGGG 
AAACCCTCTG CCTCTGATCC AGAATCCGGC GCTGCTCCCA CAGTGAACAG CAGCTCCAGA 
AGCTCTTCGC CCTCTAAGGT GATGGATGAG GGCAAGGTTA GTATGGCTGC AAAAGGGCCC 
CCTCCCTTTC CAGGGACACC CCTCATGAGC TCCCCTGTGG GAGGCCCTCT ACTGCCACCC 
ATCCGATATG GACCACCTCC TCAACTCTGT GGGCCTTTCG GACCTCGGCC ACTCCCTCCA 
CCCTTTGGTC CTGGTATGCG TCCACCGCTA GGCTTAAGAG AATATGCACC AGGTGTTCCA 
CCGGGAAAAC GGGACCTGCC TCTCGACCCT CGAGAATTTT TACCACCAGG ACATGCACCT 
TTTAGACCTT TAGGCTCTCT TGGCCCAAGA GAGTACTTTT TTCCTGGTAC CCGATTACCA 
CCCCCCAATC ATGGGCCCCA GGATTATCCA CCTTCCTCTG CTGCAAGAGA CTTACCGCCC 
TCCGGCTCCA GAGATGAGCC CCCGCCTGCC TCTCAGGGCG CTAGCCAGGA CTGCTCACCG 
GCTTTAAAAC AGAGCCCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831925.1
CDS	163..2523
Translation	MDSVPATVPSVTSSSGDPELLGPLSGLYAALIARLLELLATLPDDVQPGPDFYGLPWKPVLITASLGIVSFAVFFWRTVLAVKSRVYQVTEQQISEKLKNIMKENAELVQKLSSYEQKIKESKKHVQETKKQNMILSDEAIKFKDKIKSLEETNEILGDTAKSLRAMLESEREQNAKNQDLISENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIRSFEKSQKDLEVALTHKDDNINALTNCITQLNRLDCESESEDQNKGGSESDELANGEVGGDRSEKVKNQIKQMMDVSRTQTAISVVEEDLKLLQCKLRASMSTKCNLEDQIKKLEEDRSSLQSAKTVLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKAVLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMMQEEPVIVKPMPGRPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTADPPARPLSATLNRREMPRSEFGSVDGPLPRPRWASEASGKPSASDPESGAAPTVNSSSRSSSPSKVMDEGKVSMAAKGPPPFPGTPLMSSPVGGPLLPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREYAPGVPPGKRDLPLDPREFLPPGHAPFRPLGSLGPREYFFPGTRLPPPNHGPQDYPPSSAARDLPPSGSRDEPPPASQGASQDCSPALKQSP

Target ORF information:

RefSeq Version	XM_024976157.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976157.1

ORF Insert Sequence:

ATGGACTCAG TACCTGCCAC TGTGCCTTCT GTTACCTCTT CCTCGGGGGA CCCGGAGCTT 
CTGGGACCCC TATCGGGGCT CTACGCAGCA CTCATAGCCA GGCTCCTGGA GCTCCTTGCT 
ACATTGCCTG ATGATGTCCA GCCTGGGCCT GATTTTTATG GACTGCCATG GAAGCCTGTA 
CTTATCACTG CCTCCTTAGG AATTGTTTCA TTTGCCGTTT TCTTCTGGAG AACTGTCCTT 
GCTGTAAAGA GTAGAGTATA TCAAGTCACT GAACAGCAAA TTTCTGAGAA GTTGAAGAAT 
ATCATGAAAG AAAACGCAGA ACTTGTACAA AAATTGTCAA GTTATGAGCA GAAGATCAAG 
GAATCAAAGA AACATGTTCA AGAAACCAAG AAACAAAACA TGATTCTTTC TGATGAAGCA 
ATTAAATTTA AGGATAAAAT CAAGAGTCTT GAAGAAACTA ATGAAATTCT GGGAGACACA 
GCTAAAAGTT TACGCGCTAT GTTAGAGTCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC 
TTGATATCAG AAAATAAGAA ATCTATAGAG AAGTTAAAGG ATGTTATCTC AGTGAATGCC 
TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAAGTG 
AAGTCTGAAT GCCATCGGGT TCAAGAAGAA AATGCTAGAC TTAAGAAGAA GAAAGAGCAG 
TTGCAGCAAG AAATCAAAGA CTGGAGTAAA TCACATGCTG AGCTCAGTGA GCAAATCAGA 
TCATTTGAGA AGTCTCAGAA AGATCTAGAA GTAGCTCTCA CTCACAAAGA TGATAATATT 
AATGCTTTGA CTAATTGCAT TACACAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG 
GATCAAAATA AAGGAGGAAG TGAATCAGAT GAATTAGCAA ATGGAGAAGT GGGAGGTGAC 
CGGAGTGAGA AGGTGAAAAA TCAGATTAAG CAGATGATGG ACGTTTCTCG GACACAGACT 
GCAATATCAG TAGTTGAAGA GGATCTGAAA CTTTTACAGT GTAAACTCAG AGCCTCCATG 
TCCACTAAGT GTAACCTGGA AGACCAAATA AAGAAATTAG AAGAGGATCG CAGCTCACTT 
CAGTCTGCCA AAACTGTGCT GGAAGATGAA TGCAAAACAC TGAGGCAGAA AGTAGAGATT 
CTAAATGAAC TCTATCAGCA GAAGGAGATG GCTCTACAAA AGAAATTGAG TCAAGAAGAG 
TATGAGCGGC AAGAAAGAGA GCAGCGGCTG TCAGCTGCAG ATGAAAAGGC GGTTTTGGCG 
GCAGAGGAAG TGAAAACTTA CAAGCGGAGA ATTGAAGAAA TGGAGGATGA ACTACAGAAA 
ACAGAGCGCT CATTTAAAAA CCAGATTGCT ACTCATGAGA AGAAAGCTCA CGATAACTGG 
CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCTGAAGAGA AGAGAGAAGC TGCCAACTTG 
AGACATAAAT TATTGGAACT AACCCAAAAG ATGGCAATGA TGCAAGAAGA ACCTGTGATT 
GTAAAACCAA TGCCAGGAAG ACCCAACACA CAAAACCCTC CACGGAGAGG TCCCCTGAGC 
CAGAATGGCT CTTTTGGCCC ATCTCCTGTG AGCGGTGGAG AATGCTCCCC ACCGCTGACG 
GCAGACCCGC CCGCAAGACC TCTGTCTGCT ACGCTTAATC GAAGAGAGAT GCCTCGAAGT 
GAATTTGGAT CAGTGGATGG GCCTCTCCCT CGACCTCGAT GGGCCTCTGA GGCATCTGGG 
AAACCCTCTG CCTCTGATCC AGAATCCGGC GCTGCTCCCA CAGTGAACAG CAGCTCCAGA 
AGCTCTTCGC CCTCTAAGGT GATGGATGAG GGCAAGGTTA GTATGGCTGC AAAAGGGCCC 
CCTCCCTTTC CAGGGACACC CCTCATGAGC TCCCCTGTGG GAGGCCCTCT ACTGCCACCC 
ATCCGATATG GACCACCTCC TCAACTCTGT GGGCCTTTCG GACCTCGGCC ACTCCCTCCA 
CCCTTTGGTC CTGGTATGCG TCCACCGCTA GGCTTAAGAG AATATGCACC AGGTGTTCCA 
CCGGGAAAAC GGGACCTGCC TCTCGACCCT CGAGAATTTT TACCACCAGG ACATGCACCT 
TTTAGACCTT TAGGCTCTCT TGGCCCAAGA GAGTACTTTT TTCCTGGTAC CCGATTACCA 
CCCCCCAATC ATGGGCCCCA GGATTATCCA CCTTCCTCTG CTGCAAGAGA CTTACCGCCC 
TCCGGCTCCA GAGATGAGCC CCCGCCTGCC TCTCAGGGCG CTAGCCAGGA CTGCTCACCG 
GCTTTAAAAC AGAGCCCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( NM_001166571.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001160043.2 Bos taurus MIA SH3 domain ER export factor 3 (MIA3), mRNA.

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CloneID	OBa239258
Clone ID Related Accession (Same CDS sequence)	NM_001166571.2
Accession Version	NM_001166571.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Bos taurus(cattle)
Product	transport and Golgi organization protein 1 homolog precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000016.1. On May 30, 2018 this sequence version replaced NM_001166571.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR5571261.2599.1, SRR6764281.29576.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCGG CGCAGGGGCT GCTCTTCTGG CTGCTCCTGC TCGGGCCGCC TTGGCGCGTG 
CCCGGCCAGC CGGAGCAGGA CCCCGGCCGG CGCTTCTCGC AGCTCAAACT CTGCGCGGAC 
GAAGAATGCA GCATGCTAAT GTACCGGGGT GAGGCTCTAG AAGATTTTAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGAGAC ACTGTATATG TCTACTATAA ACTGGCAGGG 
GGATCACCTG AAGTTTGGGC TGGAAGTGTT GGCCATACTT TTGGATATTT TCCAAAGGAT 
TTAATCCAAG TAGTCCATGA ATATACTCAG GAAGAGCTGC AAGTTCCAAC AGATGAGACA 
GATTTCGTTT GTTTTGATGG GGGAAGAGAT GATTTTGATA ATTATAATGT CGAAGACCTT 
TTAGGATTTT TGGAATTGTA CGATTCTGCC ACTGAAGATT CTGAAAAAGT TAAAGAAAAA 
ACTGCTCAGC GTGTGGAAGA ACCTCCTGAA GCATCTAATG AAAGTGACGC TGAACCTGAG 
CCCGGAGAAC CCAACTCAGA GGAAAGTGAA AGCGTACTCT CAGAAAATAC TGCGGAACTC 
AGGGAAAGAT CTGAGGCTCA GAAGAGCCAC CCCCAGGTAA ACAGTCAAAC AGGTCATGCT 
CAGGGAGAGC GAACTTCATT TGAATCTTTT GGAGAAATGC TGCAAGATAA GTTAAAAGTG 
CCAGACAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCGCACGA GCAGGAGAAG 
ATCGATGCTT ATAAACTTTT GAAAACAGAA ATGACTCTGG ACTTGAAAAC CAAATTTGGC 
TCAACTGCAG ATGCACTGGT ATCTGATGAT GAGACAACCA GGCTTGTTAC TTCATTAGAG 
GACGATTTTG TTGAGGATTT GGATCCTGAG TATTACACAG TGGGAAAGGA AGAAGAGGAG 
AATAAAGAAG ACTTCGATGA GCTGCCGTTA CTAACCTTTA CAGATGGAGA AGATACGAAA 
AGTCCAGGGC ACTCCGGAAT TGAGAAACAC CCAACAGAGA AAGAGCAGAA TTCAAACAAA 
GAGCATAAAG TTGAGGAAAC TCAGCCCCCA GGCATCAAAA AGGGTGATAA AGAGATACCG 
AAACACCGGG AAGATACTGT CTTCTCTGAT GTTATGGAAG GGGAAGAAAA CACAGACACA 
GATTTAGAGA GTTCTGATTC AAAGGAAGAG GATGACCCAT TAGTCATGGA TAGCAGATTG 
GGGAAACCAC GACCAGAAGA TCATACTGAC CCTGAAAAGG CAGCAGACCA CCTTGTGAAT 
GTAGAAGTCC CCAAAGCAGA CAGTGACGAC GACCCGGAAG TGGGCGCAGG ACTTCACATG 
AAAGACAAGG GGCGGAAAGT TGAGGAGCCC AGGAGGGACT GGGTGCAGCA TGAGGTAGGG 
CTAGAGGACG AGACGCAAGA AGACCAGGCT GTGCAGGGCT CTTCTCAGAG TGGCCACCTC 
AGGTCTTCAC CAGCTGCTGA AAAAAGTACA GAAACATTAA AATCAGCCTT TGCTAACCAA 
GAGAATGACC TGAAAGGAGC AGCCGTTCAC ATCTCCAAAG AAATGCTTCA CGAAGAAAAG 
CCCTCAGGGC GGAGTTTGGA AGGTGCCTCA AAGAGTGACT CAGTCCCGCA AGCAAAAGCG 
GCGGGGAATC AAGGGGAGGA AGGAAAGACT GAACGGGAAC CTGTGGGCGT GGCAGCCCCC 
TCGGGCGATC ATCAGCCTAA TGCTTCCAAA GACAGCGTGG ACGAAGTAGA CGGTTCAATA 
AGTGGGCCTA AACCACACGT GCTTTCGGGG GAGCATCCGA GCGCAGAACT TATAAAGGAT 
CGGCTTCTTA AACTTCAAAA TCAGACCAGG TTCTCCTCTC CAGATGACAT GGGCTTGCCA 
GGAGACCTGG AAAAAAAGCG GCCCATTCTG GAAAGAAAGC TTTCCTGGCA ACAAGGAGGT 
GTGGCTGCCG CAGTTAATAA GCAAGTGAGC GAAAAGAGAG AGCTCCCTGA AGAAGAGGTC 
ACCAGGGTCA CCAAGGATGC CTCAGATGAA GGGCAGGAAG TAAGAAAAAC AGGCCAAACT 
GATAGCATAG AAGGGCGAGG CTTCCGCCCA AAAGAACCAA ACCCAGAAGA TGAAGATTAC 
AGCCCTGAAG AACTCCTGGA GGATGAAAAT GCTGTAAGTG CAAAGCAGTC TAAAGAAAGA 
AGCCCTGAGA TTCAGGATAA GAGGTTAGAT GTTGACCTGC AAAACCCTGA AAAACCTGTT 
TCAGGTGCCA TTAAGACTGA TCCAGAAACT GAAAAAAACA AAGAAGAAAC TAGGCATGTA 
TCGGAAAATG AAAGAAAAAA TGAGACTGCG GGTAAAGCGG TAGACAGTCT GGGCAGGGAC 
GCAGGTGGCC CGGTGGTGGA AAAAGAAGGA AGCTCTCCTG TGCATCAGAA GGTACAGAGA 
CCCTCTGAAG GAAGTGACGT TCCTGGCAAG AAACAAAATC AGACTCCAGA GTTAGGGGAA 
GCGTCTCAGA AGAAAGATCC TGATTATCTT AAAGAAGACA ACCATGAGGG CCACCCAAAG 
ACCTCAGGGC TCATGGAGAA GCCTGGGGTA GAACCCTCGA AAGAGGACGA CGAGCATGCA 
GAGAAATTCG TGGACCCAGG AAGCCGGGGG TCTGCTTCCG AGGACCCTGA CGATGACCCG 
TTCCCCTGGG CTCCACATGC CCCTGTGCAG CCTGAGGAGA GTGTCCATTT GGAGGACTTG 
CCCATAATCA GCAGCTTCTT TAAAGATCAG CAGTCTCTGC AGCGATTCCA GAAGTACTTT 
GACATCCACA AGCTGGAAGC CATGTTCCAG GACATGTCAT CCAAGCTGAA GTCAGCTCAG 
CGTGAGAGCC TGCCCTATAA CGTGGAGAAA GTCCTCGATA AGGTCTTTCG TGCTTGGGAG 
TCACACATTC TGACAGAGGC AGAGAACATG CTTGATGCTC GCGTGACGGA AAACAGAGAC 
CTGGAAACGA AGGATAGTAG CGTCTTTGAG GAGGCTGCTG TGCTCGATGA TGTTCAAGAC 
CTGATCTATT TCGTCAGGTA CAAACACTCC ACAGTGGAGG AGACTGCTCC CCCAGCAGCG 
GCACAGCCTG TAGAGGGAGG CTGGGATGGA CCAGCAGAAG ACACACAGCC ACCACTGGAG 
GAGAACTTCC CACAGGAGCA CATGGAAGTT CCTCTGATGC AGATTCCCAA AGAGCCCGGC 
CATTTGGCTC AACCCGTGAC CAGGGACATG GGTACCTCAG GAGTGGCCCA GAAGCCGCAA 
ACCGAGGAAG ATGGAGACCC AGGGATAATT ACACCACAAG GCACTCCTGT CGATGCTGAT 
GATGCCCAAA AGCAGCTGGC AGCAAATACT GAAGAACCAG CAAGTGTCAC GCCTCTGGAA 
AATGCAATTG CTTTCATATA CTCACTTGTG TTTCATTTAA CTAAGACGCT CCTTGCTACA 
TTGCCTGATG ATGTCCAGCC TGGGCCTGAT TTTTATGGAC TGCCATGGAA GCCTGTACTT 
ATCACTGCCT CCTTAGGAAT TGTTTCATTT GCCGTTTTCT TCTGGAGAAC TGTCCTTGCT 
GTAAAGAGTA GAGTATATCA AGTCACTGAA CAGCAAATTT CTGAGAAGTT GAAGAATATC 
ATGAAAGAAA ACGCAGAACT TGTACAAAAA TTGTCAAGTT ATGAGCAGAA GATCAAGGAA 
TCAAAGAAAC ATGTTCAAGA AACCAAGAAA CAAAACATGA TTCTTTCTGA TGAAGCAATT 
AAATTTAAGG ATAAAATCAA GAGTCTTGAA GAAACTAATG AAATTCTGGG AGACACAGCT 
AAAAGTTTAC GCGCTATGTT AGAGTCTGAG AGAGAACAGA ATGCCAAGAA TCAGGACTTG 
ATATCAGAAA ATAAGAAATC TATAGAGAAG TTAAAGGATG TTATCTCAGT GAATGCCTCA 
GAATTTTCAG AGGTTCAAAT TGCCCTTAAT GAAGCTAAAC TTAGTGAAGA GAAAGTGAAG 
TCTGAATGCC ATCGGGTTCA AGAAGAAAAT GCTAGACTTA AGAAGAAGAA AGAGCAGTTG 
CAGCAAGAAA TCAAAGACTG GAGTAAATCA CATGCTGAGC TCAGTGAGCA AATCAGATCA 
TTTGAGAAGT CTCAGAAAGA TCTAGAAGTA GCTCTCACTC ACAAAGATGA TAATATTAAT 
GCTTTGACTA ATTGCATTAC ACAGTTGAAT CGGTTAGATT GTGAATCTGA ATCTGAGGAT 
CAAAATAAAG GAGGAAGTGA ATCAGATGAA TTAGCAAATG GAGAAGTGGG AGGTGACCGG 
AGTGAGAAGG TGAAAAATCA GATTAAGCAG ATGATGGACG TTTCTCGGAC ACAGACTGCA 
ATATCAGTAG TTGAAGAGGA TCTGAAACTT TTACAGTGTA AACTCAGAGC CTCCATGTCC 
ACTAAGTGTA ACCTGGAAGA CCAAATAAAG AAATTAGAAG AGGATCGCAG CTCACTTCAG 
TCTGCCAAAA CTGTGCTGGA AGATGAATGC AAAACACTGA GGCAGAAAGT AGAGATTCTA 
AATGAACTCT ATCAGCAGAA GGAGATGGCT CTACAAAAGA AATTGAGTCA AGAAGAGTAT 
GAGCGGCAAG AAAGAGAGCA GCGGCTGTCA GCTGCAGATG AAAAGGCGGT TTTGGCGGCA 
GAGGAAGTGA AAACTTACAA GCGGAGAATT GAAGAAATGG AGGATGAACT ACAGAAAACA 
GAGCGCTCAT TTAAAAACCA GATTGCTACT CATGAGAAGA AAGCTCACGA TAACTGGCTC 
AAAGCTCGTG CTGCAGAAAG AGCTATAGCT GAAGAGAAGA GAGAAGCTGC CAACTTGAGA 
CATAAATTAT TGGAACTAAC CCAAAAGATG GCAATGATGC AAGAAGAACC TGTGATTGTA 
AAACCAATGC CAGGAAGACC CAACACACAA AACCCTCCAC GGAGAGGTCC CCTGAGCCAG 
AATGGCTCTT TTGGCCCATC TCCTGTGAGC GGTGGAGAAT GCTCCCCACC GCTGACGGCA 
GACCCGCCCG CAAGACCTCT GTCTGCTACG CTTAATCGAA GAGAGATGCC TCGAAGTGAA 
TTTGGATCAG TGGATGGGCC TCTCCCTCGA CCTCGATGGG CCTCTGAGGC ATCTGGGAAA 
CCCTCTGCCT CTGATCCAGA ATCCGGCGCT GCTCCCACAG TGAACAGCAG CTCCAGAAGC 
TCTTCGCCCT CTAAGGTGAT GGATGAGGGC AAGGTTAGTA TGGCTGCAAA AGGGCCCCCT 
CCCTTTCCAG GGACACCCCT CATGAGCTCC CCTGTGGGAG GCCCTCTACT GCCACCCATC 
CGATATGGAC CACCTCCTCA ACTCTGTGGG CCTTTCGGAC CTCGGCCACT CCCTCCACCC 
TTTGGTCCTG GTATGCGTCC ACCGCTAGGC TTAAGAGAAT ATGCACCAGG TGTTCCACCG 
GGAAAACGGG ACCTGCCTCT CGACCCTCGA GAATTTTTAC CACCAGGACA TGCACCTTTT 
AGACCTTTAG GCTCTCTTGG CCCAAGAGAG TACTTTTTTC CTGGTACCCG ATTACCACCC 
CCCAATCATG GGCCCCAGGA TTATCCACCT TCCTCTGCTG CAAGAGACTT ACCGCCCTCC 
GGCTCCAGAG ATGAGCCCCC GCCTGCCTCT CAGGGCGCTA GCCAGGACTG CTCACCGGCT 
TTAAAACAGA GCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001160043.2
CDS	1..5718
Translation	MAAAQGLLFWLLLLGPPWRVPGQPEQDPGRRFSQLKLCADEECSMLMYRGEALEDFTGPDCRFVNFKKGDTVYVYYKLAGGSPEVWAGSVGHTFGYFPKDLIQVVHEYTQEELQVPTDETDFVCFDGGRDDFDNYNVEDLLGFLELYDSATEDSEKVKEKTAQRVEEPPEASNESDAEPEPGEPNSEESESVLSENTAELRERSEAQKSHPQVNSQTGHAQGERTSFESFGEMLQDKLKVPDSENNKTSNSSQVSHEQEKIDAYKLLKTEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFVEDLDPEYYTVGKEEEENKEDFDELPLLTFTDGEDTKSPGHSGIEKHPTEKEQNSNKEHKVEETQPPGIKKGDKEIPKHREDTVFSDVMEGEENTDTDLESSDSKEEDDPLVMDSRLGKPRPEDHTDPEKAADHLVNVEVPKADSDDDPEVGAGLHMKDKGRKVEEPRRDWVQHEVGLEDETQEDQAVQGSSQSGHLRSSPAAEKSTETLKSAFANQENDLKGAAVHISKEMLHEEKPSGRSLEGASKSDSVPQAKAAGNQGEEGKTEREPVGVAAPSGDHQPNASKDSVDEVDGSISGPKPHVLSGEHPSAELIKDRLLKLQNQTRFSSPDDMGLPGDLEKKRPILERKLSWQQGGVAAAVNKQVSEKRELPEEEVTRVTKDASDEGQEVRKTGQTDSIEGRGFRPKEPNPEDEDYSPEELLEDENAVSAKQSKERSPEIQDKRLDVDLQNPEKPVSGAIKTDPETEKNKEETRHVSENERKNETAGKAVDSLGRDAGGPVVEKEGSSPVHQKVQRPSEGSDVPGKKQNQTPELGEASQKKDPDYLKEDNHEGHPKTSGLMEKPGVEPSKEDDEHAEKFVDPGSRGSASEDPDDDPFPWAPHAPVQPEESVHLEDLPIISSFFKDQQSLQRFQKYFDIHKLEAMFQDMSSKLKSAQRESLPYNVEKVLDKVFRAWESHILTEAENMLDARVTENRDLETKDSSVFEEAAVLDDVQDLIYFVRYKHSTVEETAPPAAAQPVEGGWDGPAEDTQPPLEENFPQEHMEVPLMQIPKEPGHLAQPVTRDMGTSGVAQKPQTEEDGDPGIITPQGTPVDADDAQKQLAANTEEPASVTPLENAIAFIYSLVFHLTKTLLATLPDDVQPGPDFYGLPWKPVLITASLGIVSFAVFFWRTVLAVKSRVYQVTEQQISEKLKNIMKENAELVQKLSSYEQKIKESKKHVQETKKQNMILSDEAIKFKDKIKSLEETNEILGDTAKSLRAMLESEREQNAKNQDLISENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIRSFEKSQKDLEVALTHKDDNINALTNCITQLNRLDCESESEDQNKGGSESDELANGEVGGDRSEKVKNQIKQMMDVSRTQTAISVVEEDLKLLQCKLRASMSTKCNLEDQIKKLEEDRSSLQSAKTVLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKAVLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMMQEEPVIVKPMPGRPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTADPPARPLSATLNRREMPRSEFGSVDGPLPRPRWASEASGKPSASDPESGAAPTVNSSSRSSSPSKVMDEGKVSMAAKGPPPFPGTPLMSSPVGGPLLPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREYAPGVPPGKRDLPLDPREFLPPGHAPFRPLGSLGPREYFFPGTRLPPPNHGPQDYPPSSAARDLPPSGSRDEPPPASQGASQDCSPALKQSP

Target ORF information:

RefSeq Version	NM_001166571.2
Organism	Bos taurus(cattle)
Definition	Bos taurus MIA SH3 domain ER export factor 3 (MIA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166571.2

ORF Insert Sequence:

ATGGCTGCGG CGCAGGGGCT GCTCTTCTGG CTGCTCCTGC TCGGGCCGCC TTGGCGCGTG 
CCCGGCCAGC CGGAGCAGGA CCCCGGCCGG CGCTTCTCGC AGCTCAAACT CTGCGCGGAC 
GAAGAATGCA GCATGCTAAT GTACCGGGGT GAGGCTCTAG AAGATTTTAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGAGAC ACTGTATATG TCTACTATAA ACTGGCAGGG 
GGATCACCTG AAGTTTGGGC TGGAAGTGTT GGCCATACTT TTGGATATTT TCCAAAGGAT 
TTAATCCAAG TAGTCCATGA ATATACTCAG GAAGAGCTGC AAGTTCCAAC AGATGAGACA 
GATTTCGTTT GTTTTGATGG GGGAAGAGAT GATTTTGATA ATTATAATGT CGAAGACCTT 
TTAGGATTTT TGGAATTGTA CGATTCTGCC ACTGAAGATT CTGAAAAAGT TAAAGAAAAA 
ACTGCTCAGC GTGTGGAAGA ACCTCCTGAA GCATCTAATG AAAGTGACGC TGAACCTGAG 
CCCGGAGAAC CCAACTCAGA GGAAAGTGAA AGCGTACTCT CAGAAAATAC TGCGGAACTC 
AGGGAAAGAT CTGAGGCTCA GAAGAGCCAC CCCCAGGTAA ACAGTCAAAC AGGTCATGCT 
CAGGGAGAGC GAACTTCATT TGAATCTTTT GGAGAAATGC TGCAAGATAA GTTAAAAGTG 
CCAGACAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCGCACGA GCAGGAGAAG 
ATCGATGCTT ATAAACTTTT GAAAACAGAA ATGACTCTGG ACTTGAAAAC CAAATTTGGC 
TCAACTGCAG ATGCACTGGT ATCTGATGAT GAGACAACCA GGCTTGTTAC TTCATTAGAG 
GACGATTTTG TTGAGGATTT GGATCCTGAG TATTACACAG TGGGAAAGGA AGAAGAGGAG 
AATAAAGAAG ACTTCGATGA GCTGCCGTTA CTAACCTTTA CAGATGGAGA AGATACGAAA 
AGTCCAGGGC ACTCCGGAAT TGAGAAACAC CCAACAGAGA AAGAGCAGAA TTCAAACAAA 
GAGCATAAAG TTGAGGAAAC TCAGCCCCCA GGCATCAAAA AGGGTGATAA AGAGATACCG 
AAACACCGGG AAGATACTGT CTTCTCTGAT GTTATGGAAG GGGAAGAAAA CACAGACACA 
GATTTAGAGA GTTCTGATTC AAAGGAAGAG GATGACCCAT TAGTCATGGA TAGCAGATTG 
GGGAAACCAC GACCAGAAGA TCATACTGAC CCTGAAAAGG CAGCAGACCA CCTTGTGAAT 
GTAGAAGTCC CCAAAGCAGA CAGTGACGAC GACCCGGAAG TGGGCGCAGG ACTTCACATG 
AAAGACAAGG GGCGGAAAGT TGAGGAGCCC AGGAGGGACT GGGTGCAGCA TGAGGTAGGG 
CTAGAGGACG AGACGCAAGA AGACCAGGCT GTGCAGGGCT CTTCTCAGAG TGGCCACCTC 
AGGTCTTCAC CAGCTGCTGA AAAAAGTACA GAAACATTAA AATCAGCCTT TGCTAACCAA 
GAGAATGACC TGAAAGGAGC AGCCGTTCAC ATCTCCAAAG AAATGCTTCA CGAAGAAAAG 
CCCTCAGGGC GGAGTTTGGA AGGTGCCTCA AAGAGTGACT CAGTCCCGCA AGCAAAAGCG 
GCGGGGAATC AAGGGGAGGA AGGAAAGACT GAACGGGAAC CTGTGGGCGT GGCAGCCCCC 
TCGGGCGATC ATCAGCCTAA TGCTTCCAAA GACAGCGTGG ACGAAGTAGA CGGTTCAATA 
AGTGGGCCTA AACCACACGT GCTTTCGGGG GAGCATCCGA GCGCAGAACT TATAAAGGAT 
CGGCTTCTTA AACTTCAAAA TCAGACCAGG TTCTCCTCTC CAGATGACAT GGGCTTGCCA 
GGAGACCTGG AAAAAAAGCG GCCCATTCTG GAAAGAAAGC TTTCCTGGCA ACAAGGAGGT 
GTGGCTGCCG CAGTTAATAA GCAAGTGAGC GAAAAGAGAG AGCTCCCTGA AGAAGAGGTC 
ACCAGGGTCA CCAAGGATGC CTCAGATGAA GGGCAGGAAG TAAGAAAAAC AGGCCAAACT 
GATAGCATAG AAGGGCGAGG CTTCCGCCCA AAAGAACCAA ACCCAGAAGA TGAAGATTAC 
AGCCCTGAAG AACTCCTGGA GGATGAAAAT GCTGTAAGTG CAAAGCAGTC TAAAGAAAGA 
AGCCCTGAGA TTCAGGATAA GAGGTTAGAT GTTGACCTGC AAAACCCTGA AAAACCTGTT 
TCAGGTGCCA TTAAGACTGA TCCAGAAACT GAAAAAAACA AAGAAGAAAC TAGGCATGTA 
TCGGAAAATG AAAGAAAAAA TGAGACTGCG GGTAAAGCGG TAGACAGTCT GGGCAGGGAC 
GCAGGTGGCC CGGTGGTGGA AAAAGAAGGA AGCTCTCCTG TGCATCAGAA GGTACAGAGA 
CCCTCTGAAG GAAGTGACGT TCCTGGCAAG AAACAAAATC AGACTCCAGA GTTAGGGGAA 
GCGTCTCAGA AGAAAGATCC TGATTATCTT AAAGAAGACA ACCATGAGGG CCACCCAAAG 
ACCTCAGGGC TCATGGAGAA GCCTGGGGTA GAACCCTCGA AAGAGGACGA CGAGCATGCA 
GAGAAATTCG TGGACCCAGG AAGCCGGGGG TCTGCTTCCG AGGACCCTGA CGATGACCCG 
TTCCCCTGGG CTCCACATGC CCCTGTGCAG CCTGAGGAGA GTGTCCATTT GGAGGACTTG 
CCCATAATCA GCAGCTTCTT TAAAGATCAG CAGTCTCTGC AGCGATTCCA GAAGTACTTT 
GACATCCACA AGCTGGAAGC CATGTTCCAG GACATGTCAT CCAAGCTGAA GTCAGCTCAG 
CGTGAGAGCC TGCCCTATAA CGTGGAGAAA GTCCTCGATA AGGTCTTTCG TGCTTGGGAG 
TCACACATTC TGACAGAGGC AGAGAACATG CTTGATGCTC GCGTGACGGA AAACAGAGAC 
CTGGAAACGA AGGATAGTAG CGTCTTTGAG GAGGCTGCTG TGCTCGATGA TGTTCAAGAC 
CTGATCTATT TCGTCAGGTA CAAACACTCC ACAGTGGAGG AGACTGCTCC CCCAGCAGCG 
GCACAGCCTG TAGAGGGAGG CTGGGATGGA CCAGCAGAAG ACACACAGCC ACCACTGGAG 
GAGAACTTCC CACAGGAGCA CATGGAAGTT CCTCTGATGC AGATTCCCAA AGAGCCCGGC 
CATTTGGCTC AACCCGTGAC CAGGGACATG GGTACCTCAG GAGTGGCCCA GAAGCCGCAA 
ACCGAGGAAG ATGGAGACCC AGGGATAATT ACACCACAAG GCACTCCTGT CGATGCTGAT 
GATGCCCAAA AGCAGCTGGC AGCAAATACT GAAGAACCAG CAAGTGTCAC GCCTCTGGAA 
AATGCAATTG CTTTCATATA CTCACTTGTG TTTCATTTAA CTAAGACGCT CCTTGCTACA 
TTGCCTGATG ATGTCCAGCC TGGGCCTGAT TTTTATGGAC TGCCATGGAA GCCTGTACTT 
ATCACTGCCT CCTTAGGAAT TGTTTCATTT GCCGTTTTCT TCTGGAGAAC TGTCCTTGCT 
GTAAAGAGTA GAGTATATCA AGTCACTGAA CAGCAAATTT CTGAGAAGTT GAAGAATATC 
ATGAAAGAAA ACGCAGAACT TGTACAAAAA TTGTCAAGTT ATGAGCAGAA GATCAAGGAA 
TCAAAGAAAC ATGTTCAAGA AACCAAGAAA CAAAACATGA TTCTTTCTGA TGAAGCAATT 
AAATTTAAGG ATAAAATCAA GAGTCTTGAA GAAACTAATG AAATTCTGGG AGACACAGCT 
AAAAGTTTAC GCGCTATGTT AGAGTCTGAG AGAGAACAGA ATGCCAAGAA TCAGGACTTG 
ATATCAGAAA ATAAGAAATC TATAGAGAAG TTAAAGGATG TTATCTCAGT GAATGCCTCA 
GAATTTTCAG AGGTTCAAAT TGCCCTTAAT GAAGCTAAAC TTAGTGAAGA GAAAGTGAAG 
TCTGAATGCC ATCGGGTTCA AGAAGAAAAT GCTAGACTTA AGAAGAAGAA AGAGCAGTTG 
CAGCAAGAAA TCAAAGACTG GAGTAAATCA CATGCTGAGC TCAGTGAGCA AATCAGATCA 
TTTGAGAAGT CTCAGAAAGA TCTAGAAGTA GCTCTCACTC ACAAAGATGA TAATATTAAT 
GCTTTGACTA ATTGCATTAC ACAGTTGAAT CGGTTAGATT GTGAATCTGA ATCTGAGGAT 
CAAAATAAAG GAGGAAGTGA ATCAGATGAA TTAGCAAATG GAGAAGTGGG AGGTGACCGG 
AGTGAGAAGG TGAAAAATCA GATTAAGCAG ATGATGGACG TTTCTCGGAC ACAGACTGCA 
ATATCAGTAG TTGAAGAGGA TCTGAAACTT TTACAGTGTA AACTCAGAGC CTCCATGTCC 
ACTAAGTGTA ACCTGGAAGA CCAAATAAAG AAATTAGAAG AGGATCGCAG CTCACTTCAG 
TCTGCCAAAA CTGTGCTGGA AGATGAATGC AAAACACTGA GGCAGAAAGT AGAGATTCTA 
AATGAACTCT ATCAGCAGAA GGAGATGGCT CTACAAAAGA AATTGAGTCA AGAAGAGTAT 
GAGCGGCAAG AAAGAGAGCA GCGGCTGTCA GCTGCAGATG AAAAGGCGGT TTTGGCGGCA 
GAGGAAGTGA AAACTTACAA GCGGAGAATT GAAGAAATGG AGGATGAACT ACAGAAAACA 
GAGCGCTCAT TTAAAAACCA GATTGCTACT CATGAGAAGA AAGCTCACGA TAACTGGCTC 
AAAGCTCGTG CTGCAGAAAG AGCTATAGCT GAAGAGAAGA GAGAAGCTGC CAACTTGAGA 
CATAAATTAT TGGAACTAAC CCAAAAGATG GCAATGATGC AAGAAGAACC TGTGATTGTA 
AAACCAATGC CAGGAAGACC CAACACACAA AACCCTCCAC GGAGAGGTCC CCTGAGCCAG 
AATGGCTCTT TTGGCCCATC TCCTGTGAGC GGTGGAGAAT GCTCCCCACC GCTGACGGCA 
GACCCGCCCG CAAGACCTCT GTCTGCTACG CTTAATCGAA GAGAGATGCC TCGAAGTGAA 
TTTGGATCAG TGGATGGGCC TCTCCCTCGA CCTCGATGGG CCTCTGAGGC ATCTGGGAAA 
CCCTCTGCCT CTGATCCAGA ATCCGGCGCT GCTCCCACAG TGAACAGCAG CTCCAGAAGC 
TCTTCGCCCT CTAAGGTGAT GGATGAGGGC AAGGTTAGTA TGGCTGCAAA AGGGCCCCCT 
CCCTTTCCAG GGACACCCCT CATGAGCTCC CCTGTGGGAG GCCCTCTACT GCCACCCATC 
CGATATGGAC CACCTCCTCA ACTCTGTGGG CCTTTCGGAC CTCGGCCACT CCCTCCACCC 
TTTGGTCCTG GTATGCGTCC ACCGCTAGGC TTAAGAGAAT ATGCACCAGG TGTTCCACCG 
GGAAAACGGG ACCTGCCTCT CGACCCTCGA GAATTTTTAC CACCAGGACA TGCACCTTTT 
AGACCTTTAG GCTCTCTTGG CCCAAGAGAG TACTTTTTTC CTGGTACCCG ATTACCACCC 
CCCAATCATG GGCCCCAGGA TTATCCACCT TCCTCTGCTG CAAGAGACTT ACCGCCCTCC 
GGCTCCAGAG ATGAGCCCCC GCCTGCCTCT CAGGGCGCTA GCCAGGACTG CTCACCGGCT 
TTAAAACAGA GCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_024976152.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831920.1 Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa223509
Clone ID Related Accession (Same CDS sequence)	XM_024976152.1
Accession Version	XM_024976152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5790bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transport and Golgi organization protein 1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG CGCAGGGGCT GCTCTTCTGG CTGCTCCTGC TCGGGCCGCC TTGGCGCGTG 
CCCGGCCAGC CGGAGCAGGA CCCCGGCCGG CGCTTCTCGC AGCTCAAACT CTGCGCGGAC 
GAAGAATGCA GCATGCTAAT GTACCGGGGT GAGGCTCTAG AAGATTTTAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGAGAC ACTGTATATG TCTACTATAA ACTGGCAGGG 
GGATCACCTG AAGTTTGGGC TGGAAGTGTT GGCCATACTT TTGGATATTT TCCAAAGGAT 
TTAATCCAAG TAGTCCATGA ATATACTCAG GAAGAGCTGC AAGTTCCAAC AGATGAGACA 
GATTTCGTTT GTTTTGATGG GGGAAGAGAT GATTTTGATA ATTATAATGT CGAAGACCTT 
TTAGGATTTT TGGAATTGTA CGATTCTGCC ACTGAAGATT CTGAAAAAGT TAAAGAAAAA 
ACTGCTCAGC GTGTGGAAGA ACCTCCTGAA GCATCTAATG AAAGTGACGC TGAACCTGAG 
CCCGGAGAAC CCAACTCAGA GGAAAGTGAA AGCGTACTCT CAGAAAATAC TGCGGAACTC 
AGGGAAAGAT CTGAGGCTCA GAAGAGCCAC CCCCAGGTAA ACAGTCAAAC AGGTCATGCT 
CAGGGAGAGC GAACTTCATT TGAATCTTTT GGAGAAATGC TGCAAGATAA GTTAAAAGTG 
CCAGACAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCGCACGA GCAGGAGAAG 
ATCGATGCTT ATAAACTTTT GAAAACAGAA ATGACTCTGG ACTTGAAAAC CAAATTTGGC 
TCAACTGCAG ATGCACTGGT ATCTGATGAT GAGACAACCA GGCTTGTTAC TTCATTAGAG 
GACGATTTTG TTGAGGATTT GGATCCTGAG TATTACACAG TGGGAAAGGA AGAAGAGGAG 
AATAAAGAAG ACTTCGATGA GCTGCCGTTA CTAACCTTTA CAGATGGAGA AGATACGAAA 
AGTCCAGGGC ACTCCGGAAT TGAGAAACAC CCAACAGAGA AAGAGCAGAA TTCAAACAAA 
GAGCATAAAG TTGAGGAAAC TCAGCCCCCA GGCATCAAAA AGGGTGATAA AGAGATACCG 
AAACACCGGG AAGATACTGT CTTCTCTGAT GTTATGGAAG GGGAAGAAAA CACAGACACA 
GATTTAGAGA GTTCTGATTC AAAGGAAGAG GATGACCCAT TAGTCATGGA TAGCAGATTG 
GGGAAACCAC GACCAGAAGA TCATACTGAC CCTGAAAAGG CAGCAGACCA CCTTGTGAAT 
GTAGAAGTCC CCAAAGCAGA CAGTGACGAC GACCCGGAAG TGGGCGCAGG ACTTCACATG 
AAAGACAAGG GGCGGAAAGT TGAGGAGCCC AGGAGGGACT GGGTGCAGCA TGAGGTAGGG 
CTAGAGGACG AGACGCAAGA AGACCAGGCT GTGCAGGGCT CTTCTCAGAG TGGCCACCTC 
AGGTCTTCAC CAGCTGCTGA AAAAAGTACA GAAACATTAA AATCAGCCTT TGCTAACCAA 
GAGAATGACC TGAAAGGAGC AGCCGTTCAC ATCTCCAAAG AAATGCTTCA CGAAGAAAAG 
CCCTCAGGGC GGAGTTTGGA AGGTGCCTCA AAGAGTGACT CAGTCCCGCA AGCAAAAGCG 
GCGGGGAATC AAGGGGAGGA AGGAAAGACT GAACGGGAAC CTGTGGGCGT GGCAGCCCCC 
TCGGGCGATC ATCAGCCTAA TGCTTCCAAA GACAGCGTGG ACGAAGTAGA CGGTTCAATA 
AGTGGGCCTA AACCACACGT GCTTTCGGGG GAGCATCCGA GCGCAGAACT TATAAAGGAT 
CGGCTTCTTA AACTTCAAAA TCAGACCAGG TTCTCCTCTC CAGATGACAT GGGCTTGCCA 
GGAGACCTGG AAAAAAAGCG GCCCATTCTG GAAAGAAAGC TTTCCTGGCA ACAAGGAGGT 
GTGGCTGCCG CAGTTAATAA GCAAGTGAGC GAAAAGAGAG AGCTCCCTGA AGAAGAGGTC 
ACCAGGGTCA CCAAGGATGC CTCAGATGAA GGGCAGGAAG TAAGAAAAAC AGGCCAAACT 
GATAGCATAG AAGGGCGAGG CTTCCGCCCA AAAGAACCAA ACCCAGAAGA TGAAGATTAC 
AGCCCTGAAG AACTCCTGGA GGATGAAAAT GCTGTAAGTG CAAAGCAGTC TAAAGAAAGA 
AGCCCTGAGA TTCAGGATAA GAGGTTAGAT GTTGACCTGC AAAACCCTGA AAAACCTGTT 
TCAGGTGCCA TTAAGACTGA TCCAGAAACT GAAAAAAACA AAGAAGAAAC TAGGCATGTA 
TCGGAAAATG AAAGAAAAAA TGAGACTGCG GGTAAAGCGG TAGACAGTCT GGGCAGGGAC 
GCAGGTGGCC CGGTGGTGGA AAAAGAAGGA AGCTCTCCTG TGCATCAGAA GGTACAGAGA 
CCCTCTGAAG GAAGTGACGT TCCTGGCAAG AAACAAAATC AGACTCCAGA GTTAGGGGAA 
GCGTCTCAGA AGAAAGATCC TGATTATCTT AAAGAAGACA ACCATGAGGG CCACCCAAAG 
ACCTCAGGGC TCATGGAGAA GCCTGGGGTA GAACCCTCGA AAGAGGACGA CGAGCATGCA 
GAGAAATTCG TGGACCCAGG AAGCCGGGGG TCTGCTTCCG AGGACCCTGA CGATGACCCG 
TTCCCCTGGG CTCCACATGC CCCTGTGCAG CCTGAGGAGA GTGTCCATTT GGAGGACTTG 
CCCATAATCA GCAGCTTCTT TAAAGATCAG CAGTCTCTGC AGCGATTCCA GAAGTACTTT 
GACATCCACA AGCTGGAAGC CATGTTCCAG GACATGTCAT CCAAGCTGAA GTCAGCTCAG 
CGTGAGAGCC TGCCCTATAA CGTGGAGAAA GTCCTCGATA AGGTCTTTCG TGCTTGGGAG 
TCACACATTC TGACAGAGGC AGAGAACATG CTTGATGCTC GCGTGACGGA AAACAGAGAC 
CTGGAAACGA AGGATAGTAG CGTCTTTGAG GAGGCTGCTG TGCTCGATGA TGTTCAAGAC 
CTGATCTATT TCGTCAGGTA CAAACACTCC ACAGTGGAGG AGACTGCTCC CCCAGCAGCG 
GCACAGCCTG TAGAGGGAGG CTGGGATGGA CCAGCAGAAG ACACACAGCC ACCACTGGAG 
GAGAACTTCC CACAGGAGCA CATGGAAGTT CCTCTGATGC AGATTCCCAA AGAGCCCGGC 
CATTTGGCTC AACCCGTGAC CAGGGACATG GGTACCTCAG GAGTGGCCCA GAAGCCGCAA 
ACCGAGGAAG ATGGAGACCC AGGGATAATT ACACCACAAG GCACTCCTGT CGATGCTGAT 
GATGCCCAAA AGCAGCTGGC AGCAAATACT GAAGAACCAG CAAGTGTCAC GCCTCTGGAA 
AATGCAATTG CTTTCATATA CTCACTTGTG TTTCATTTAA CTAAGACGCT CCTTGCTACA 
TTGCCTGATG ATGTCCAGCC TGGGCCTGAT TTTTATGGAC TGCCATGGAA GCCTGTACTT 
ATCACTGCCT CCTTAGGAAT TGTTTCATTT GCCGTTTTCT TCTGGAGAAC TGTCCTTGCT 
GTAAAGAGTA GAGTATATCA AGTCACTGAA CAGCAAATTT CTGAGAAGTT GAAGAATATC 
ATGAAAGAAA ACGCAGAACT TGTACAAAAA TTGTCAAGTT ATGAGCAGAA GATCAAGGAA 
TCAAAGAAAC ATGTTCAAGA AACCAAGAAA CAAAACATGA TTCTTTCTGA TGAAGCAATT 
AAATTTAAGG ATAAAATCAA GAGTCTTGAA GAAACTAATG AAATTCTGGG AGACACAGCT 
AAAAGTTTAC GCGCTATGTT AGAGTCTGAG AGAGAACAGA ATGCCAAGAA TCAGGACTTG 
ATATCAGAAA ATAAGAAATC TATAGAGAAG TTAAAGGATG TTATCTCAGT GAATGCCTCA 
GAATTTTCAG AGGTTCAAAT TGCCCTTAAT GAAGCTAAAC TTAGTGAAGA GAAAGTGAAG 
TCTGAATGCC ATCGGGTTCA AGAAGAAAAT GCTAGACTTA AGAAGAAGAA AGAGCAGTTG 
CAGCAAGAAA TCAAAGACTG GAGTAAATCA CATGCTGAGC TCAGTGAGCA AATCAGATCA 
TTTGAGAAGT CTCAGAAAGA TCTAGAAGTA GCTCTCACTC ACAAAGATGA TAATATTAAT 
GCTTTGACTA ATTGCATTAC ACAGTTGAAT CGGTTAGATT GTGAATCTGA ATCTGAGGAT 
CAAAATAAAG GAGGAAGTGA ATCAGATGAA TTAGCAAATG GAGAAGTGGG AGGTGACCGG 
AGTGAGAAGG TGAAAAATCA GATTAAGCAG ATGATGGACG TTTCTCGGAC ACAGACTGCA 
ATATCAGTAG TTGAAGAGGA TCTGAAACTT TTACAGTGTA AACTCAGAGC CTCCATGTCC 
ACTAAGTGTA ACCTGGAAGA CCAAATAAAG AAATTAGAAG AGGATCGCAG CTCACTTCAG 
TCTGCCAAAA CTGTGCTGGA AGATGAATGC AAAACACTGA GGCAGAAAGT AGAGATTCTA 
AATGAACTCT ATCAGCAGAA GGAGATGGCT CTACAAAAGA AATTGAGTCA AGAAGAGTAT 
GAGCGGCAAG AAAGAGAGCA GCGGCTGTCA GCTGCAGATG AAAAGGCGGT TTTGGCGGCA 
GAGGAAGTGA AAACTTACAA GCGGAGAATT GAAGAAATGG AGGATGAACT ACAGAAAACA 
GAGCGCTCAT TTAAAAACCA GATTGCTACT CATGAGAAGA AAGCTCACGA TAACTGGCTC 
AAAGCTCGTG CTGCAGAAAG AGCTATAGCT GAAGAGAAGA GAGAAGCTGC CAACTTGAGA 
CATAAATTAT TGGAACTAAC CCAAAAGATG GCAATGATGC AAGAAGAACC TGTGATTGTA 
AAACCAATGC CAGGAAGACC CAACACACAA AACCCTCCAC GGAGAGGTCC CCTGAGCCAG 
AATGGCTCTT TTGGCCCATC TCCTGTGAGC GGTGGAGAAT GCTCCCCACC GCTGACGGCA 
GACCCGCCCG CAAGACCTCT GTCTGCTACG CTTAATCGAA GAGAGATGCC TCGAAGTGAA 
TTTGGATCAG TGGATGGGCC TCTCCCTCGA CCTCGATGGG CCTCTGAGGC ATCTGGGAAA 
CCCTCTGCCT CTGATCCAGA ATCCGGCGCT GCTCCCACAG TGAACAGCAG CTCCAGAAGC 
TCTTCGCCCT CTAAGGTGAT GGATGAGGGC AAGCAAACTG TTCCCCAAGA GCCTGAAGGC 
CCTTCAGTTC CCAGCATTCC TTCTTTGGCT GAGCATCCAG TATCAGTTAG TATGGCTGCA 
AAAGGGCCCC CTCCCTTTCC AGGGACACCC CTCATGAGCT CCCCTGTGGG AGGCCCTCTA 
CTGCCACCCA TCCGATATGG ACCACCTCCT CAACTCTGTG GGCCTTTCGG ACCTCGGCCA 
CTCCCTCCAC CCTTTGGTCC TGGTATGCGT CCACCGCTAG GCTTAAGAGA ATATGCACCA 
GGTGTTCCAC CGGGAAAACG GGACCTGCCT CTCGACCCTC GAGAATTTTT ACCACCAGGA 
CATGCACCTT TTAGACCTTT AGGCTCTCTT GGCCCAAGAG AGTACTTTTT TCCTGGTACC 
CGATTACCAC CCCCCAATCA TGGGCCCCAG GATTATCCAC CTTCCTCTGC TGCAAGAGAC 
TTACCGCCCT CCGGCTCCAG AGATGAGCCC CCGCCTGCCT CTCAGGGCGC TAGCCAGGAC 
TGCTCACCGG CTTTAAAACA GAGCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831920.1
CDS	59..5848
Translation	MAAAQGLLFWLLLLGPPWRVPGQPEQDPGRRFSQLKLCADEECSMLMYRGEALEDFTGPDCRFVNFKKGDTVYVYYKLAGGSPEVWAGSVGHTFGYFPKDLIQVVHEYTQEELQVPTDETDFVCFDGGRDDFDNYNVEDLLGFLELYDSATEDSEKVKEKTAQRVEEPPEASNESDAEPEPGEPNSEESESVLSENTAELRERSEAQKSHPQVNSQTGHAQGERTSFESFGEMLQDKLKVPDSENNKTSNSSQVSHEQEKIDAYKLLKTEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFVEDLDPEYYTVGKEEEENKEDFDELPLLTFTDGEDTKSPGHSGIEKHPTEKEQNSNKEHKVEETQPPGIKKGDKEIPKHREDTVFSDVMEGEENTDTDLESSDSKEEDDPLVMDSRLGKPRPEDHTDPEKAADHLVNVEVPKADSDDDPEVGAGLHMKDKGRKVEEPRRDWVQHEVGLEDETQEDQAVQGSSQSGHLRSSPAAEKSTETLKSAFANQENDLKGAAVHISKEMLHEEKPSGRSLEGASKSDSVPQAKAAGNQGEEGKTEREPVGVAAPSGDHQPNASKDSVDEVDGSISGPKPHVLSGEHPSAELIKDRLLKLQNQTRFSSPDDMGLPGDLEKKRPILERKLSWQQGGVAAAVNKQVSEKRELPEEEVTRVTKDASDEGQEVRKTGQTDSIEGRGFRPKEPNPEDEDYSPEELLEDENAVSAKQSKERSPEIQDKRLDVDLQNPEKPVSGAIKTDPETEKNKEETRHVSENERKNETAGKAVDSLGRDAGGPVVEKEGSSPVHQKVQRPSEGSDVPGKKQNQTPELGEASQKKDPDYLKEDNHEGHPKTSGLMEKPGVEPSKEDDEHAEKFVDPGSRGSASEDPDDDPFPWAPHAPVQPEESVHLEDLPIISSFFKDQQSLQRFQKYFDIHKLEAMFQDMSSKLKSAQRESLPYNVEKVLDKVFRAWESHILTEAENMLDARVTENRDLETKDSSVFEEAAVLDDVQDLIYFVRYKHSTVEETAPPAAAQPVEGGWDGPAEDTQPPLEENFPQEHMEVPLMQIPKEPGHLAQPVTRDMGTSGVAQKPQTEEDGDPGIITPQGTPVDADDAQKQLAANTEEPASVTPLENAIAFIYSLVFHLTKTLLATLPDDVQPGPDFYGLPWKPVLITASLGIVSFAVFFWRTVLAVKSRVYQVTEQQISEKLKNIMKENAELVQKLSSYEQKIKESKKHVQETKKQNMILSDEAIKFKDKIKSLEETNEILGDTAKSLRAMLESEREQNAKNQDLISENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIRSFEKSQKDLEVALTHKDDNINALTNCITQLNRLDCESESEDQNKGGSESDELANGEVGGDRSEKVKNQIKQMMDVSRTQTAISVVEEDLKLLQCKLRASMSTKCNLEDQIKKLEEDRSSLQSAKTVLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKAVLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMMQEEPVIVKPMPGRPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTADPPARPLSATLNRREMPRSEFGSVDGPLPRPRWASEASGKPSASDPESGAAPTVNSSSRSSSPSKVMDEGKQTVPQEPEGPSVPSIPSLAEHPVSVSMAAKGPPPFPGTPLMSSPVGGPLLPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREYAPGVPPGKRDLPLDPREFLPPGHAPFRPLGSLGPREYFFPGTRLPPPNHGPQDYPPSSAARDLPPSGSRDEPPPASQGASQDCSPALKQSP

Target ORF information:

RefSeq Version	XM_024976152.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976152.1

ORF Insert Sequence:

ATGGCTGCGG CGCAGGGGCT GCTCTTCTGG CTGCTCCTGC TCGGGCCGCC TTGGCGCGTG 
CCCGGCCAGC CGGAGCAGGA CCCCGGCCGG CGCTTCTCGC AGCTCAAACT CTGCGCGGAC 
GAAGAATGCA GCATGCTAAT GTACCGGGGT GAGGCTCTAG AAGATTTTAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGAGAC ACTGTATATG TCTACTATAA ACTGGCAGGG 
GGATCACCTG AAGTTTGGGC TGGAAGTGTT GGCCATACTT TTGGATATTT TCCAAAGGAT 
TTAATCCAAG TAGTCCATGA ATATACTCAG GAAGAGCTGC AAGTTCCAAC AGATGAGACA 
GATTTCGTTT GTTTTGATGG GGGAAGAGAT GATTTTGATA ATTATAATGT CGAAGACCTT 
TTAGGATTTT TGGAATTGTA CGATTCTGCC ACTGAAGATT CTGAAAAAGT TAAAGAAAAA 
ACTGCTCAGC GTGTGGAAGA ACCTCCTGAA GCATCTAATG AAAGTGACGC TGAACCTGAG 
CCCGGAGAAC CCAACTCAGA GGAAAGTGAA AGCGTACTCT CAGAAAATAC TGCGGAACTC 
AGGGAAAGAT CTGAGGCTCA GAAGAGCCAC CCCCAGGTAA ACAGTCAAAC AGGTCATGCT 
CAGGGAGAGC GAACTTCATT TGAATCTTTT GGAGAAATGC TGCAAGATAA GTTAAAAGTG 
CCAGACAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCGCACGA GCAGGAGAAG 
ATCGATGCTT ATAAACTTTT GAAAACAGAA ATGACTCTGG ACTTGAAAAC CAAATTTGGC 
TCAACTGCAG ATGCACTGGT ATCTGATGAT GAGACAACCA GGCTTGTTAC TTCATTAGAG 
GACGATTTTG TTGAGGATTT GGATCCTGAG TATTACACAG TGGGAAAGGA AGAAGAGGAG 
AATAAAGAAG ACTTCGATGA GCTGCCGTTA CTAACCTTTA CAGATGGAGA AGATACGAAA 
AGTCCAGGGC ACTCCGGAAT TGAGAAACAC CCAACAGAGA AAGAGCAGAA TTCAAACAAA 
GAGCATAAAG TTGAGGAAAC TCAGCCCCCA GGCATCAAAA AGGGTGATAA AGAGATACCG 
AAACACCGGG AAGATACTGT CTTCTCTGAT GTTATGGAAG GGGAAGAAAA CACAGACACA 
GATTTAGAGA GTTCTGATTC AAAGGAAGAG GATGACCCAT TAGTCATGGA TAGCAGATTG 
GGGAAACCAC GACCAGAAGA TCATACTGAC CCTGAAAAGG CAGCAGACCA CCTTGTGAAT 
GTAGAAGTCC CCAAAGCAGA CAGTGACGAC GACCCGGAAG TGGGCGCAGG ACTTCACATG 
AAAGACAAGG GGCGGAAAGT TGAGGAGCCC AGGAGGGACT GGGTGCAGCA TGAGGTAGGG 
CTAGAGGACG AGACGCAAGA AGACCAGGCT GTGCAGGGCT CTTCTCAGAG TGGCCACCTC 
AGGTCTTCAC CAGCTGCTGA AAAAAGTACA GAAACATTAA AATCAGCCTT TGCTAACCAA 
GAGAATGACC TGAAAGGAGC AGCCGTTCAC ATCTCCAAAG AAATGCTTCA CGAAGAAAAG 
CCCTCAGGGC GGAGTTTGGA AGGTGCCTCA AAGAGTGACT CAGTCCCGCA AGCAAAAGCG 
GCGGGGAATC AAGGGGAGGA AGGAAAGACT GAACGGGAAC CTGTGGGCGT GGCAGCCCCC 
TCGGGCGATC ATCAGCCTAA TGCTTCCAAA GACAGCGTGG ACGAAGTAGA CGGTTCAATA 
AGTGGGCCTA AACCACACGT GCTTTCGGGG GAGCATCCGA GCGCAGAACT TATAAAGGAT 
CGGCTTCTTA AACTTCAAAA TCAGACCAGG TTCTCCTCTC CAGATGACAT GGGCTTGCCA 
GGAGACCTGG AAAAAAAGCG GCCCATTCTG GAAAGAAAGC TTTCCTGGCA ACAAGGAGGT 
GTGGCTGCCG CAGTTAATAA GCAAGTGAGC GAAAAGAGAG AGCTCCCTGA AGAAGAGGTC 
ACCAGGGTCA CCAAGGATGC CTCAGATGAA GGGCAGGAAG TAAGAAAAAC AGGCCAAACT 
GATAGCATAG AAGGGCGAGG CTTCCGCCCA AAAGAACCAA ACCCAGAAGA TGAAGATTAC 
AGCCCTGAAG AACTCCTGGA GGATGAAAAT GCTGTAAGTG CAAAGCAGTC TAAAGAAAGA 
AGCCCTGAGA TTCAGGATAA GAGGTTAGAT GTTGACCTGC AAAACCCTGA AAAACCTGTT 
TCAGGTGCCA TTAAGACTGA TCCAGAAACT GAAAAAAACA AAGAAGAAAC TAGGCATGTA 
TCGGAAAATG AAAGAAAAAA TGAGACTGCG GGTAAAGCGG TAGACAGTCT GGGCAGGGAC 
GCAGGTGGCC CGGTGGTGGA AAAAGAAGGA AGCTCTCCTG TGCATCAGAA GGTACAGAGA 
CCCTCTGAAG GAAGTGACGT TCCTGGCAAG AAACAAAATC AGACTCCAGA GTTAGGGGAA 
GCGTCTCAGA AGAAAGATCC TGATTATCTT AAAGAAGACA ACCATGAGGG CCACCCAAAG 
ACCTCAGGGC TCATGGAGAA GCCTGGGGTA GAACCCTCGA AAGAGGACGA CGAGCATGCA 
GAGAAATTCG TGGACCCAGG AAGCCGGGGG TCTGCTTCCG AGGACCCTGA CGATGACCCG 
TTCCCCTGGG CTCCACATGC CCCTGTGCAG CCTGAGGAGA GTGTCCATTT GGAGGACTTG 
CCCATAATCA GCAGCTTCTT TAAAGATCAG CAGTCTCTGC AGCGATTCCA GAAGTACTTT 
GACATCCACA AGCTGGAAGC CATGTTCCAG GACATGTCAT CCAAGCTGAA GTCAGCTCAG 
CGTGAGAGCC TGCCCTATAA CGTGGAGAAA GTCCTCGATA AGGTCTTTCG TGCTTGGGAG 
TCACACATTC TGACAGAGGC AGAGAACATG CTTGATGCTC GCGTGACGGA AAACAGAGAC 
CTGGAAACGA AGGATAGTAG CGTCTTTGAG GAGGCTGCTG TGCTCGATGA TGTTCAAGAC 
CTGATCTATT TCGTCAGGTA CAAACACTCC ACAGTGGAGG AGACTGCTCC CCCAGCAGCG 
GCACAGCCTG TAGAGGGAGG CTGGGATGGA CCAGCAGAAG ACACACAGCC ACCACTGGAG 
GAGAACTTCC CACAGGAGCA CATGGAAGTT CCTCTGATGC AGATTCCCAA AGAGCCCGGC 
CATTTGGCTC AACCCGTGAC CAGGGACATG GGTACCTCAG GAGTGGCCCA GAAGCCGCAA 
ACCGAGGAAG ATGGAGACCC AGGGATAATT ACACCACAAG GCACTCCTGT CGATGCTGAT 
GATGCCCAAA AGCAGCTGGC AGCAAATACT GAAGAACCAG CAAGTGTCAC GCCTCTGGAA 
AATGCAATTG CTTTCATATA CTCACTTGTG TTTCATTTAA CTAAGACGCT CCTTGCTACA 
TTGCCTGATG ATGTCCAGCC TGGGCCTGAT TTTTATGGAC TGCCATGGAA GCCTGTACTT 
ATCACTGCCT CCTTAGGAAT TGTTTCATTT GCCGTTTTCT TCTGGAGAAC TGTCCTTGCT 
GTAAAGAGTA GAGTATATCA AGTCACTGAA CAGCAAATTT CTGAGAAGTT GAAGAATATC 
ATGAAAGAAA ACGCAGAACT TGTACAAAAA TTGTCAAGTT ATGAGCAGAA GATCAAGGAA 
TCAAAGAAAC ATGTTCAAGA AACCAAGAAA CAAAACATGA TTCTTTCTGA TGAAGCAATT 
AAATTTAAGG ATAAAATCAA GAGTCTTGAA GAAACTAATG AAATTCTGGG AGACACAGCT 
AAAAGTTTAC GCGCTATGTT AGAGTCTGAG AGAGAACAGA ATGCCAAGAA TCAGGACTTG 
ATATCAGAAA ATAAGAAATC TATAGAGAAG TTAAAGGATG TTATCTCAGT GAATGCCTCA 
GAATTTTCAG AGGTTCAAAT TGCCCTTAAT GAAGCTAAAC TTAGTGAAGA GAAAGTGAAG 
TCTGAATGCC ATCGGGTTCA AGAAGAAAAT GCTAGACTTA AGAAGAAGAA AGAGCAGTTG 
CAGCAAGAAA TCAAAGACTG GAGTAAATCA CATGCTGAGC TCAGTGAGCA AATCAGATCA 
TTTGAGAAGT CTCAGAAAGA TCTAGAAGTA GCTCTCACTC ACAAAGATGA TAATATTAAT 
GCTTTGACTA ATTGCATTAC ACAGTTGAAT CGGTTAGATT GTGAATCTGA ATCTGAGGAT 
CAAAATAAAG GAGGAAGTGA ATCAGATGAA TTAGCAAATG GAGAAGTGGG AGGTGACCGG 
AGTGAGAAGG TGAAAAATCA GATTAAGCAG ATGATGGACG TTTCTCGGAC ACAGACTGCA 
ATATCAGTAG TTGAAGAGGA TCTGAAACTT TTACAGTGTA AACTCAGAGC CTCCATGTCC 
ACTAAGTGTA ACCTGGAAGA CCAAATAAAG AAATTAGAAG AGGATCGCAG CTCACTTCAG 
TCTGCCAAAA CTGTGCTGGA AGATGAATGC AAAACACTGA GGCAGAAAGT AGAGATTCTA 
AATGAACTCT ATCAGCAGAA GGAGATGGCT CTACAAAAGA AATTGAGTCA AGAAGAGTAT 
GAGCGGCAAG AAAGAGAGCA GCGGCTGTCA GCTGCAGATG AAAAGGCGGT TTTGGCGGCA 
GAGGAAGTGA AAACTTACAA GCGGAGAATT GAAGAAATGG AGGATGAACT ACAGAAAACA 
GAGCGCTCAT TTAAAAACCA GATTGCTACT CATGAGAAGA AAGCTCACGA TAACTGGCTC 
AAAGCTCGTG CTGCAGAAAG AGCTATAGCT GAAGAGAAGA GAGAAGCTGC CAACTTGAGA 
CATAAATTAT TGGAACTAAC CCAAAAGATG GCAATGATGC AAGAAGAACC TGTGATTGTA 
AAACCAATGC CAGGAAGACC CAACACACAA AACCCTCCAC GGAGAGGTCC CCTGAGCCAG 
AATGGCTCTT TTGGCCCATC TCCTGTGAGC GGTGGAGAAT GCTCCCCACC GCTGACGGCA 
GACCCGCCCG CAAGACCTCT GTCTGCTACG CTTAATCGAA GAGAGATGCC TCGAAGTGAA 
TTTGGATCAG TGGATGGGCC TCTCCCTCGA CCTCGATGGG CCTCTGAGGC ATCTGGGAAA 
CCCTCTGCCT CTGATCCAGA ATCCGGCGCT GCTCCCACAG TGAACAGCAG CTCCAGAAGC 
TCTTCGCCCT CTAAGGTGAT GGATGAGGGC AAGCAAACTG TTCCCCAAGA GCCTGAAGGC 
CCTTCAGTTC CCAGCATTCC TTCTTTGGCT GAGCATCCAG TATCAGTTAG TATGGCTGCA 
AAAGGGCCCC CTCCCTTTCC AGGGACACCC CTCATGAGCT CCCCTGTGGG AGGCCCTCTA 
CTGCCACCCA TCCGATATGG ACCACCTCCT CAACTCTGTG GGCCTTTCGG ACCTCGGCCA 
CTCCCTCCAC CCTTTGGTCC TGGTATGCGT CCACCGCTAG GCTTAAGAGA ATATGCACCA 
GGTGTTCCAC CGGGAAAACG GGACCTGCCT CTCGACCCTC GAGAATTTTT ACCACCAGGA 
CATGCACCTT TTAGACCTTT AGGCTCTCTT GGCCCAAGAG AGTACTTTTT TCCTGGTACC 
CGATTACCAC CCCCCAATCA TGGGCCCCAG GATTATCCAC CTTCCTCTGC TGCAAGAGAC 
TTACCGCCCT CCGGCTCCAG AGATGAGCCC CCGCCTGCCT CTCAGGGCGC TAGCCAGGAC 
TGCTCACCGG CTTTAAAACA GAGCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_024976153.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831921.1 Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa223510
Clone ID Related Accession (Same CDS sequence)	XM_024976153.1
Accession Version	XM_024976153.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transport and Golgi organization protein 1 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG CGCAGGGGCT GCTCTTCTGG CTGCTCCTGC TCGGGCCGCC TTGGCGCGTG 
CCCGGCCAGC CGGAGCAGGA CCCCGGCCGG CGCTTCTCGC AGCTCAAACT CTGCGCGGAC 
GAAGAATGCA GCATGCTAAT GTACCGGGGT GAGGCTCTAG AAGATTTTAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGAGAC ACTGTATATG TCTACTATAA ACTGGCAGGG 
GGATCACCTG AAGTTTGGGC TGGAAGTGTT GGCCATACTT TTGGATATTT TCCAAAGGAT 
TTAATCCAAG TAGTCCATGA ATATACTCAG GAAGAGCTGC AAGTTCCAAC AGATGAGACA 
GATTTCGTTT GTTTTGATGG GGGAAGAGAT GATTTTGATA ATTATAATGT CGAAGACCTT 
TTAGGATTTT TGGAATTGTA CGATTCTGCC ACTGAAGATT CTGAAAAAGT TAAAGAAAAA 
ACTGCTCAGC GTGTGGAAGA ACCTCCTGAA GCATCTAATG AAAGTGACGC TGAACCTGAG 
CCCGGAGAAC CCAACTCAGA GGAAAGTGAA AGCGTACTCT CAGAAAATAC TGCGGAACTC 
AGGGAAAGAT CTGAGGCTCA GAAGAGCCAC CCCCAGGTAA ACAGTCAAAC AGGTCATGCT 
CAGGGAGAGC GAACTTCATT TGAATCTTTT GGAGAAATGC TGCAAGATAA GTTAAAAGTG 
CCAGACAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCGCACGA GCAGGAGAAG 
ATCGATGCTT ATAAACTTTT GAAAACAGAA ATGACTCTGG ACTTGAAAAC CAAATTTGGC 
TCAACTGCAG ATGCACTGGT ATCTGATGAT GAGACAACCA GGCTTGTTAC TTCATTAGAG 
GACGATTTTG TTGAGGATTT GGATCCTGAG TATTACACAG TGGGAAAGGA AGAAGAGGAG 
AATAAAGAAG ACTTCGATGA GCTGCCGTTA CTAACCTTTA CAGATGGAGA AGATACGAAA 
AGTCCAGGGC ACTCCGGAAT TGAGAAACAC CCAACAGAGA AAGAGCAGAA TTCAAACAAA 
GAGCATAAAG TTGAGGAAAC TCAGCCCCCA GGCATCAAAA AGGGTGATAA AGAGATACCG 
AAACACCGGG AAGATACTGT CTTCTCTGAT GTTATGGAAG GGGAAGAAAA CACAGACACA 
GATTTAGAGA GTTCTGATTC AAAGGAAGAG GATGACCCAT TAGTCATGGA TAGCAGATTG 
GGGAAACCAC GACCAGAAGA TCATACTGAC CCTGAAAAGG CAGCAGACCA CCTTGTGAAT 
GTAGAAGTCC CCAAAGCAGA CAGTGACGAC GACCCGGAAG TGGGCGCAGG ACTTCACATG 
AAAGACAAGG GGCGGAAAGT TGAGGAGCCC AGGAGGGACT GGGTGCAGCA TGAGGTAGGG 
CTAGAGGACG AGACGCAAGA AGACCAGGCT GTGCAGGGCT CTTCTCAGAG TGGCCACCTC 
AGGTCTTCAC CAGCTGCTGA AAAAAGTACA GAAACATTAA AATCAGCCTT TGCTAACCAA 
GAGAATGACC TGAAAGGAGC AGCCGTTCAC ATCTCCAAAG AAATGCTTCA CGAAGAAAAG 
CCCTCAGGGC GGAGTTTGGA AGGTGCCTCA AAGAGTGACT CAGTCCCGCA AGCAAAAGCG 
GCGGGGAATC AAGGGGAGGA AGGAAAGACT GAACGGGAAC CTGTGGGCGT GGCAGCCCCC 
TCGGGCGATC ATCAGCCTAA TGCTTCCAAA GACAGCGTGG ACGAAGTAGA CGGTTCAATA 
AGTGGGCCTA AACCACACGT GCTTTCGGGG GAGCATCCGA GCGCAGAACT TATAAAGGAT 
CGGCTTCTTA AACTTCAAAA TCAGACCAGG TTCTCCTCTC CAGATGACAT GGGCTTGCCA 
GGAGACCTGG AAAAAAAGCG GCCCATTCTG GAAAGAAAGC TTTCCTGGCA ACAAGGAGGT 
GTGGCTGCCG CAGTTAATAA GCAAGTGAGC GAAAAGAGAG AGCTCCCTGA AGAAGAGGTC 
ACCAGGGTCA CCAAGGATGC CTCAGATGAA GGGCAGGAAG TAAGAAAAAC AGGCCAAACT 
GATAGCATAG AAGGGCGAGG CTTCCGCCCA AAAGAACCAA ACCCAGAAGA TGAAGATTAC 
AGCCCTGAAG AACTCCTGGA GGATGAAAAT GCTGTAAGTG CAAAGCAGTC TAAAGAAAGA 
AGCCCTGAGA TTCAGGATAA GAGGTTAGAT GTTGACCTGC AAAACCCTGA AAAACCTGTT 
TCAGGTGCCA TTAAGACTGA TCCAGAAACT GAAAAAAACA AAGAAGAAAC TAGGCATGTA 
TCGGAAAATG AAAGAAAAAA TGAGACTGCG GGTAAAGCGG TAGACAGTCT GGGCAGGGAC 
GCAGGTGGCC CGGTGGTGGA AAAAGAAGGA AGCTCTCCTG TGCATCAGAA GGTACAGAGA 
CCCTCTGAAG GAAGTGACGT TCCTGGCAAG AAACAAAATC AGACTCCAGA GTTAGGGGAA 
GCGTCTCAGA AGAAAGATCC TGATTATCTT AAAGAAGACA ACCATGAGGG CCACCCAAAG 
ACCTCAGGGC TCATGGAGAA GCCTGGGGTA GAACCCTCGA AAGAGGACGA CGAGCATGCA 
GAGAAATTCG TGGACCCAGG AAGCCGGGGG TCTGCTTCCG AGGACCCTGA CGATGACCCG 
TTCCCCTGGG CTCCACATGC CCCTGTGCAG CCTGAGGAGA GTGTCCATTT GGAGGACTTG 
CCCATAATCA GCAGCTTCTT TAAAGATCAG CAGTCTCTGC AGCGATTCCA GAAGTACTTT 
GACATCCACA AGCTGGAAGC CATGTTCCAG GACATGTCAT CCAAGCTGAA GTCAGCTCAG 
CGTGAGAGCC TGCCCTATAA CGTGGAGAAA GTCCTCGATA AGGTCTTTCG TGCTTGGGAG 
TCACACATTC TGACAGAGGC AGAGAACATG CTTGATGCTC GCGTGACGGA AAACAGAGAC 
CTGGAAACGA AGGATAGTAG CGTCTTTGAG GAGGCTGCTG TGCTCGATGA TGTTCAAGAC 
CTGATCTATT TCGTCAGGTA CAAACACTCC ACAGTGGAGG AGACTGCTCC CCCAGCAGCG 
GCACAGCCTG TAGAGGGAGG CTGGGATGGA CCAGCAGAAG ACACACAGCC ACCACTGGAG 
GAGAACTTCC CACAGGAGCA CATGGAAGTT CCTCTGATGC AGATTCCCAA AGAGCCCGGC 
CATTTGGCTC AACCCGTGAC CAGGGACATG GGTACCTCAG GAGTGGCCCA GAAGCCGCAA 
ACCGAGGAAG ATGGAGACCC AGGGATAATT ACACCACAAG GCACTCCTGT CGATGCTGAT 
GATGCCCAAA AGCAGCTGGC AGCAAATACT GAAGAACCAG CAAGTGTCAC GCCTCTGGAA 
AATGCAATTG CTTTCATATA CTCACTTGTG TTTCATTTAA CTAAGACGCT CCTTGCTACA 
TTGCCTGATG ATGTCCAGCC TGGGCCTGAT TTTTATGGAC TGCCATGGAA GCCTGTACTT 
ATCACTGCCT CCTTAGGAAT TGTTTCATTT GCCGTTTTCT TCTGGAGAAC TGTCCTTGCT 
GTAAAGAGTA GAGTATATCA AGTCACTGAA CAGCAAATTT CTGAGAAGTT GAAGAATATC 
ATGAAAGAAA ACGCAGAACT TGTACAAAAA TTGTCAAGTT ATGAGCAGAA GATCAAGGAA 
TCAAAGAAAC ATGTTCAAGA AACCAAGAAA CAAAACATGA TTCTTTCTGA TGAAGCAATT 
AAATTTAAGG ATAAAATCAA GAGTCTTGAA GAAACTAATG AAATTCTGGG AGACACAGCT 
AAAAGTTTAC GCGCTATGTT AGAGTCTGAG AGAGAACAGA ATGCCAAGAA TCAGGACTTG 
ATATCAGAAA ATAAGAAATC TATAGAGAAG TTAAAGGATG TTATCTCAGT GAATGCCTCA 
GAATTTTCAG AGGTTCAAAT TGCCCTTAAT GAAGCTAAAC TTAGTGAAGA GAAAGTGAAG 
TCTGAATGCC ATCGGGTTCA AGAAGAAAAT GCTAGACTTA AGAAGAAGAA AGAGCAGTTG 
CAGCAAGAAA TCAAAGACTG GAGTAAATCA CATGCTGAGC TCAGTGAGCA AATCAGATCA 
TTTGAGAAGT CTCAGAAAGA TCTAGAAGTA GCTCTCACTC ACAAAGATGA TAATATTAAT 
GCTTTGACTA ATTGCATTAC ACAGTTGAAT CGGTTAGATT GTGAATCTGA ATCTGAGGAT 
CAAAATAAAG GAGGAAGTGA ATCAGATGAA TTAGCAAATG GAGAAGTGGG AGGTGACCGG 
AGTGAGAAGG TGAAAAATCA GATTAAGCAG ATGATGGACG TTTCTCGGAC ACAGACTGCA 
ATATCAGTAG TTGAAGAGGA TCTGAAACTT TTACAGTGTA AACTCAGAGC CTCCATGTCC 
ACTAAGTGTA ACCTGGAAGA CCAAATAAAG AAATTAGAAG AGGATCGCAG CTCACTTCAG 
TCTGCCAAAA CTGTGCTGGA AGATGAATGC AAAACACTGA GGCAGAAAGT AGAGATTCTA 
AATGAACTCT ATCAGCAGAA GGAGATGGCT CTACAAAAGA AATTGAGTCA AGAAGAGTAT 
GAGCGGCAAG AAAGAGAGCA GCGGCTGTCA GCTGCAGATG AAAAGGCGGT TTTGGCGGCA 
GAGGAAGTGA AAACTTACAA GCGGAGAATT GAAGAAATGG AGGATGAACT ACAGAAAACA 
GAGCGCTCAT TTAAAAACCA GATTGCTACT CATGAGAAGA AAGCTCACGA TAACTGGCTC 
AAAGCTCGTG CTGCAGAAAG AGCTATAGCT GAAGAGAAGA GAGAAGCTGC CAACTTGAGA 
CATAAATTAT TGGAACTAAC CCAAAAGATG GCAATGATGC AAGAAGAACC TGTGATTGTA 
AAACCAATGC CAGGAAGACC CAACACACAA AACCCTCCAC GGAGAGGTCC CCTGAGCCAG 
AATGGCTCTT TTGGCCCATC TCCTGTGAGC GGTGGAGAAT GCTCCCCACC GCTGACGGCA 
GACCCGCCCG CAAGACCTCT GTCTGCTACG CTTAATCGAA GAGAGATGCC TCGAAGTGAA 
TTTGATCCAG AATCCGGCGC TGCTCCCACA GTGAACAGCA GCTCCAGAAG CTCTTCGCCC 
TCTAAGGTGA TGGATGAGGG CAAGCAAACT GTTCCCCAAG AGCCTGAAGG CCCTTCAGTT 
CCCAGCATTC CTTCTTTGGC TGAGCATCCA GTATCAGTTA GTATGGCTGC AAAAGGGCCC 
CCTCCCTTTC CAGGGACACC CCTCATGAGC TCCCCTGTGG GAGGCCCTCT ACTGCCACCC 
ATCCGATATG GACCACCTCC TCAACTCTGT GGGCCTTTCG GACCTCGGCC ACTCCCTCCA 
CCCTTTGGTC CTGGTATGCG TCCACCGCTA GGCTTAAGAG AATATGCACC AGGTGTTCCA 
CCGGGAAAAC GGGACCTGCC TCTCGACCCT CGAGAATTTT TACCACCAGG ACATGCACCT 
TTTAGACCTT TAGGCTCTCT TGGCCCAAGA GAGTACTTTT TTCCTGGTAC CCGATTACCA 
CCCCCCAATC ATGGGCCCCA GGATTATCCA CCTTCCTCTG CTGCAAGAGA CTTACCGCCC 
TCCGGCTCCA GAGATGAGCC CCCGCCTGCC TCTCAGGGCG CTAGCCAGGA CTGCTCACCG 
GCTTTAAAAC AGAGCCCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831921.1
CDS	59..5779
Translation	MAAAQGLLFWLLLLGPPWRVPGQPEQDPGRRFSQLKLCADEECSMLMYRGEALEDFTGPDCRFVNFKKGDTVYVYYKLAGGSPEVWAGSVGHTFGYFPKDLIQVVHEYTQEELQVPTDETDFVCFDGGRDDFDNYNVEDLLGFLELYDSATEDSEKVKEKTAQRVEEPPEASNESDAEPEPGEPNSEESESVLSENTAELRERSEAQKSHPQVNSQTGHAQGERTSFESFGEMLQDKLKVPDSENNKTSNSSQVSHEQEKIDAYKLLKTEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFVEDLDPEYYTVGKEEEENKEDFDELPLLTFTDGEDTKSPGHSGIEKHPTEKEQNSNKEHKVEETQPPGIKKGDKEIPKHREDTVFSDVMEGEENTDTDLESSDSKEEDDPLVMDSRLGKPRPEDHTDPEKAADHLVNVEVPKADSDDDPEVGAGLHMKDKGRKVEEPRRDWVQHEVGLEDETQEDQAVQGSSQSGHLRSSPAAEKSTETLKSAFANQENDLKGAAVHISKEMLHEEKPSGRSLEGASKSDSVPQAKAAGNQGEEGKTEREPVGVAAPSGDHQPNASKDSVDEVDGSISGPKPHVLSGEHPSAELIKDRLLKLQNQTRFSSPDDMGLPGDLEKKRPILERKLSWQQGGVAAAVNKQVSEKRELPEEEVTRVTKDASDEGQEVRKTGQTDSIEGRGFRPKEPNPEDEDYSPEELLEDENAVSAKQSKERSPEIQDKRLDVDLQNPEKPVSGAIKTDPETEKNKEETRHVSENERKNETAGKAVDSLGRDAGGPVVEKEGSSPVHQKVQRPSEGSDVPGKKQNQTPELGEASQKKDPDYLKEDNHEGHPKTSGLMEKPGVEPSKEDDEHAEKFVDPGSRGSASEDPDDDPFPWAPHAPVQPEESVHLEDLPIISSFFKDQQSLQRFQKYFDIHKLEAMFQDMSSKLKSAQRESLPYNVEKVLDKVFRAWESHILTEAENMLDARVTENRDLETKDSSVFEEAAVLDDVQDLIYFVRYKHSTVEETAPPAAAQPVEGGWDGPAEDTQPPLEENFPQEHMEVPLMQIPKEPGHLAQPVTRDMGTSGVAQKPQTEEDGDPGIITPQGTPVDADDAQKQLAANTEEPASVTPLENAIAFIYSLVFHLTKTLLATLPDDVQPGPDFYGLPWKPVLITASLGIVSFAVFFWRTVLAVKSRVYQVTEQQISEKLKNIMKENAELVQKLSSYEQKIKESKKHVQETKKQNMILSDEAIKFKDKIKSLEETNEILGDTAKSLRAMLESEREQNAKNQDLISENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIRSFEKSQKDLEVALTHKDDNINALTNCITQLNRLDCESESEDQNKGGSESDELANGEVGGDRSEKVKNQIKQMMDVSRTQTAISVVEEDLKLLQCKLRASMSTKCNLEDQIKKLEEDRSSLQSAKTVLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKAVLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMMQEEPVIVKPMPGRPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTADPPARPLSATLNRREMPRSEFDPESGAAPTVNSSSRSSSPSKVMDEGKQTVPQEPEGPSVPSIPSLAEHPVSVSMAAKGPPPFPGTPLMSSPVGGPLLPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREYAPGVPPGKRDLPLDPREFLPPGHAPFRPLGSLGPREYFFPGTRLPPPNHGPQDYPPSSAARDLPPSGSRDEPPPASQGASQDCSPALKQSP

Target ORF information:

RefSeq Version	XM_024976153.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976153.1

ORF Insert Sequence:

ATGGCTGCGG CGCAGGGGCT GCTCTTCTGG CTGCTCCTGC TCGGGCCGCC TTGGCGCGTG 
CCCGGCCAGC CGGAGCAGGA CCCCGGCCGG CGCTTCTCGC AGCTCAAACT CTGCGCGGAC 
GAAGAATGCA GCATGCTAAT GTACCGGGGT GAGGCTCTAG AAGATTTTAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGAGAC ACTGTATATG TCTACTATAA ACTGGCAGGG 
GGATCACCTG AAGTTTGGGC TGGAAGTGTT GGCCATACTT TTGGATATTT TCCAAAGGAT 
TTAATCCAAG TAGTCCATGA ATATACTCAG GAAGAGCTGC AAGTTCCAAC AGATGAGACA 
GATTTCGTTT GTTTTGATGG GGGAAGAGAT GATTTTGATA ATTATAATGT CGAAGACCTT 
TTAGGATTTT TGGAATTGTA CGATTCTGCC ACTGAAGATT CTGAAAAAGT TAAAGAAAAA 
ACTGCTCAGC GTGTGGAAGA ACCTCCTGAA GCATCTAATG AAAGTGACGC TGAACCTGAG 
CCCGGAGAAC CCAACTCAGA GGAAAGTGAA AGCGTACTCT CAGAAAATAC TGCGGAACTC 
AGGGAAAGAT CTGAGGCTCA GAAGAGCCAC CCCCAGGTAA ACAGTCAAAC AGGTCATGCT 
CAGGGAGAGC GAACTTCATT TGAATCTTTT GGAGAAATGC TGCAAGATAA GTTAAAAGTG 
CCAGACAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCGCACGA GCAGGAGAAG 
ATCGATGCTT ATAAACTTTT GAAAACAGAA ATGACTCTGG ACTTGAAAAC CAAATTTGGC 
TCAACTGCAG ATGCACTGGT ATCTGATGAT GAGACAACCA GGCTTGTTAC TTCATTAGAG 
GACGATTTTG TTGAGGATTT GGATCCTGAG TATTACACAG TGGGAAAGGA AGAAGAGGAG 
AATAAAGAAG ACTTCGATGA GCTGCCGTTA CTAACCTTTA CAGATGGAGA AGATACGAAA 
AGTCCAGGGC ACTCCGGAAT TGAGAAACAC CCAACAGAGA AAGAGCAGAA TTCAAACAAA 
GAGCATAAAG TTGAGGAAAC TCAGCCCCCA GGCATCAAAA AGGGTGATAA AGAGATACCG 
AAACACCGGG AAGATACTGT CTTCTCTGAT GTTATGGAAG GGGAAGAAAA CACAGACACA 
GATTTAGAGA GTTCTGATTC AAAGGAAGAG GATGACCCAT TAGTCATGGA TAGCAGATTG 
GGGAAACCAC GACCAGAAGA TCATACTGAC CCTGAAAAGG CAGCAGACCA CCTTGTGAAT 
GTAGAAGTCC CCAAAGCAGA CAGTGACGAC GACCCGGAAG TGGGCGCAGG ACTTCACATG 
AAAGACAAGG GGCGGAAAGT TGAGGAGCCC AGGAGGGACT GGGTGCAGCA TGAGGTAGGG 
CTAGAGGACG AGACGCAAGA AGACCAGGCT GTGCAGGGCT CTTCTCAGAG TGGCCACCTC 
AGGTCTTCAC CAGCTGCTGA AAAAAGTACA GAAACATTAA AATCAGCCTT TGCTAACCAA 
GAGAATGACC TGAAAGGAGC AGCCGTTCAC ATCTCCAAAG AAATGCTTCA CGAAGAAAAG 
CCCTCAGGGC GGAGTTTGGA AGGTGCCTCA AAGAGTGACT CAGTCCCGCA AGCAAAAGCG 
GCGGGGAATC AAGGGGAGGA AGGAAAGACT GAACGGGAAC CTGTGGGCGT GGCAGCCCCC 
TCGGGCGATC ATCAGCCTAA TGCTTCCAAA GACAGCGTGG ACGAAGTAGA CGGTTCAATA 
AGTGGGCCTA AACCACACGT GCTTTCGGGG GAGCATCCGA GCGCAGAACT TATAAAGGAT 
CGGCTTCTTA AACTTCAAAA TCAGACCAGG TTCTCCTCTC CAGATGACAT GGGCTTGCCA 
GGAGACCTGG AAAAAAAGCG GCCCATTCTG GAAAGAAAGC TTTCCTGGCA ACAAGGAGGT 
GTGGCTGCCG CAGTTAATAA GCAAGTGAGC GAAAAGAGAG AGCTCCCTGA AGAAGAGGTC 
ACCAGGGTCA CCAAGGATGC CTCAGATGAA GGGCAGGAAG TAAGAAAAAC AGGCCAAACT 
GATAGCATAG AAGGGCGAGG CTTCCGCCCA AAAGAACCAA ACCCAGAAGA TGAAGATTAC 
AGCCCTGAAG AACTCCTGGA GGATGAAAAT GCTGTAAGTG CAAAGCAGTC TAAAGAAAGA 
AGCCCTGAGA TTCAGGATAA GAGGTTAGAT GTTGACCTGC AAAACCCTGA AAAACCTGTT 
TCAGGTGCCA TTAAGACTGA TCCAGAAACT GAAAAAAACA AAGAAGAAAC TAGGCATGTA 
TCGGAAAATG AAAGAAAAAA TGAGACTGCG GGTAAAGCGG TAGACAGTCT GGGCAGGGAC 
GCAGGTGGCC CGGTGGTGGA AAAAGAAGGA AGCTCTCCTG TGCATCAGAA GGTACAGAGA 
CCCTCTGAAG GAAGTGACGT TCCTGGCAAG AAACAAAATC AGACTCCAGA GTTAGGGGAA 
GCGTCTCAGA AGAAAGATCC TGATTATCTT AAAGAAGACA ACCATGAGGG CCACCCAAAG 
ACCTCAGGGC TCATGGAGAA GCCTGGGGTA GAACCCTCGA AAGAGGACGA CGAGCATGCA 
GAGAAATTCG TGGACCCAGG AAGCCGGGGG TCTGCTTCCG AGGACCCTGA CGATGACCCG 
TTCCCCTGGG CTCCACATGC CCCTGTGCAG CCTGAGGAGA GTGTCCATTT GGAGGACTTG 
CCCATAATCA GCAGCTTCTT TAAAGATCAG CAGTCTCTGC AGCGATTCCA GAAGTACTTT 
GACATCCACA AGCTGGAAGC CATGTTCCAG GACATGTCAT CCAAGCTGAA GTCAGCTCAG 
CGTGAGAGCC TGCCCTATAA CGTGGAGAAA GTCCTCGATA AGGTCTTTCG TGCTTGGGAG 
TCACACATTC TGACAGAGGC AGAGAACATG CTTGATGCTC GCGTGACGGA AAACAGAGAC 
CTGGAAACGA AGGATAGTAG CGTCTTTGAG GAGGCTGCTG TGCTCGATGA TGTTCAAGAC 
CTGATCTATT TCGTCAGGTA CAAACACTCC ACAGTGGAGG AGACTGCTCC CCCAGCAGCG 
GCACAGCCTG TAGAGGGAGG CTGGGATGGA CCAGCAGAAG ACACACAGCC ACCACTGGAG 
GAGAACTTCC CACAGGAGCA CATGGAAGTT CCTCTGATGC AGATTCCCAA AGAGCCCGGC 
CATTTGGCTC AACCCGTGAC CAGGGACATG GGTACCTCAG GAGTGGCCCA GAAGCCGCAA 
ACCGAGGAAG ATGGAGACCC AGGGATAATT ACACCACAAG GCACTCCTGT CGATGCTGAT 
GATGCCCAAA AGCAGCTGGC AGCAAATACT GAAGAACCAG CAAGTGTCAC GCCTCTGGAA 
AATGCAATTG CTTTCATATA CTCACTTGTG TTTCATTTAA CTAAGACGCT CCTTGCTACA 
TTGCCTGATG ATGTCCAGCC TGGGCCTGAT TTTTATGGAC TGCCATGGAA GCCTGTACTT 
ATCACTGCCT CCTTAGGAAT TGTTTCATTT GCCGTTTTCT TCTGGAGAAC TGTCCTTGCT 
GTAAAGAGTA GAGTATATCA AGTCACTGAA CAGCAAATTT CTGAGAAGTT GAAGAATATC 
ATGAAAGAAA ACGCAGAACT TGTACAAAAA TTGTCAAGTT ATGAGCAGAA GATCAAGGAA 
TCAAAGAAAC ATGTTCAAGA AACCAAGAAA CAAAACATGA TTCTTTCTGA TGAAGCAATT 
AAATTTAAGG ATAAAATCAA GAGTCTTGAA GAAACTAATG AAATTCTGGG AGACACAGCT 
AAAAGTTTAC GCGCTATGTT AGAGTCTGAG AGAGAACAGA ATGCCAAGAA TCAGGACTTG 
ATATCAGAAA ATAAGAAATC TATAGAGAAG TTAAAGGATG TTATCTCAGT GAATGCCTCA 
GAATTTTCAG AGGTTCAAAT TGCCCTTAAT GAAGCTAAAC TTAGTGAAGA GAAAGTGAAG 
TCTGAATGCC ATCGGGTTCA AGAAGAAAAT GCTAGACTTA AGAAGAAGAA AGAGCAGTTG 
CAGCAAGAAA TCAAAGACTG GAGTAAATCA CATGCTGAGC TCAGTGAGCA AATCAGATCA 
TTTGAGAAGT CTCAGAAAGA TCTAGAAGTA GCTCTCACTC ACAAAGATGA TAATATTAAT 
GCTTTGACTA ATTGCATTAC ACAGTTGAAT CGGTTAGATT GTGAATCTGA ATCTGAGGAT 
CAAAATAAAG GAGGAAGTGA ATCAGATGAA TTAGCAAATG GAGAAGTGGG AGGTGACCGG 
AGTGAGAAGG TGAAAAATCA GATTAAGCAG ATGATGGACG TTTCTCGGAC ACAGACTGCA 
ATATCAGTAG TTGAAGAGGA TCTGAAACTT TTACAGTGTA AACTCAGAGC CTCCATGTCC 
ACTAAGTGTA ACCTGGAAGA CCAAATAAAG AAATTAGAAG AGGATCGCAG CTCACTTCAG 
TCTGCCAAAA CTGTGCTGGA AGATGAATGC AAAACACTGA GGCAGAAAGT AGAGATTCTA 
AATGAACTCT ATCAGCAGAA GGAGATGGCT CTACAAAAGA AATTGAGTCA AGAAGAGTAT 
GAGCGGCAAG AAAGAGAGCA GCGGCTGTCA GCTGCAGATG AAAAGGCGGT TTTGGCGGCA 
GAGGAAGTGA AAACTTACAA GCGGAGAATT GAAGAAATGG AGGATGAACT ACAGAAAACA 
GAGCGCTCAT TTAAAAACCA GATTGCTACT CATGAGAAGA AAGCTCACGA TAACTGGCTC 
AAAGCTCGTG CTGCAGAAAG AGCTATAGCT GAAGAGAAGA GAGAAGCTGC CAACTTGAGA 
CATAAATTAT TGGAACTAAC CCAAAAGATG GCAATGATGC AAGAAGAACC TGTGATTGTA 
AAACCAATGC CAGGAAGACC CAACACACAA AACCCTCCAC GGAGAGGTCC CCTGAGCCAG 
AATGGCTCTT TTGGCCCATC TCCTGTGAGC GGTGGAGAAT GCTCCCCACC GCTGACGGCA 
GACCCGCCCG CAAGACCTCT GTCTGCTACG CTTAATCGAA GAGAGATGCC TCGAAGTGAA 
TTTGATCCAG AATCCGGCGC TGCTCCCACA GTGAACAGCA GCTCCAGAAG CTCTTCGCCC 
TCTAAGGTGA TGGATGAGGG CAAGCAAACT GTTCCCCAAG AGCCTGAAGG CCCTTCAGTT 
CCCAGCATTC CTTCTTTGGC TGAGCATCCA GTATCAGTTA GTATGGCTGC AAAAGGGCCC 
CCTCCCTTTC CAGGGACACC CCTCATGAGC TCCCCTGTGG GAGGCCCTCT ACTGCCACCC 
ATCCGATATG GACCACCTCC TCAACTCTGT GGGCCTTTCG GACCTCGGCC ACTCCCTCCA 
CCCTTTGGTC CTGGTATGCG TCCACCGCTA GGCTTAAGAG AATATGCACC AGGTGTTCCA 
CCGGGAAAAC GGGACCTGCC TCTCGACCCT CGAGAATTTT TACCACCAGG ACATGCACCT 
TTTAGACCTT TAGGCTCTCT TGGCCCAAGA GAGTACTTTT TTCCTGGTAC CCGATTACCA 
CCCCCCAATC ATGGGCCCCA GGATTATCCA CCTTCCTCTG CTGCAAGAGA CTTACCGCCC 
TCCGGCTCCA GAGATGAGCC CCCGCCTGCC TCTCAGGGCG CTAGCCAGGA CTGCTCACCG 
GCTTTAAAAC AGAGCCCATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_024976154.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831922.1 Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X3, mRNA.

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CloneID	OBa223511
Clone ID Related Accession (Same CDS sequence)	XM_024976154.1
Accession Version	XM_024976154.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5652bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transport and Golgi organization protein 1 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCGGG GTGAGGCTCT AGAAGATTTT ACAGGCCCGG ATTGTCGTTT TGTGAATTTT 
AAAAAAGGAG ACACTGTATA TGTCTACTAT AAACTGGCAG GGGGATCACC TGAAGTTTGG 
GCTGGAAGTG TTGGCCATAC TTTTGGATAT TTTCCAAAGG ATTTAATCCA AGTAGTCCAT 
GAATATACTC AGGAAGAGCT GCAAGTTCCA ACAGATGAGA CAGATTTCGT TTGTTTTGAT 
GGGGGAAGAG ATGATTTTGA TAATTATAAT GTCGAAGACC TTTTAGGATT TTTGGAATTG 
TACGATTCTG CCACTGAAGA TTCTGAAAAA GTTAAAGAAA AAACTGCTCA GCGTGTGGAA 
GAACCTCCTG AAGCATCTAA TGAAAGTGAC GCTGAACCTG AGCCCGGAGA ACCCAACTCA 
GAGGAAAGTG AAAGCGTACT CTCAGAAAAT ACTGCGGAAC TCAGGGAAAG ATCTGAGGCT 
CAGAAGAGCC ACCCCCAGGT AAACAGTCAA ACAGGTCATG CTCAGGGAGA GCGAACTTCA 
TTTGAATCTT TTGGAGAAAT GCTGCAAGAT AAGTTAAAAG TGCCAGACAG TGAAAACAAC 
AAAACCAGCA ATAGTTCTCA GGTCTCGCAC GAGCAGGAGA AGATCGATGC TTATAAACTT 
TTGAAAACAG AAATGACTCT GGACTTGAAA ACCAAATTTG GCTCAACTGC AGATGCACTG 
GTATCTGATG ATGAGACAAC CAGGCTTGTT ACTTCATTAG AGGACGATTT TGTTGAGGAT 
TTGGATCCTG AGTATTACAC AGTGGGAAAG GAAGAAGAGG AGAATAAAGA AGACTTCGAT 
GAGCTGCCGT TACTAACCTT TACAGATGGA GAAGATACGA AAAGTCCAGG GCACTCCGGA 
ATTGAGAAAC ACCCAACAGA GAAAGAGCAG AATTCAAACA AAGAGCATAA AGTTGAGGAA 
ACTCAGCCCC CAGGCATCAA AAAGGGTGAT AAAGAGATAC CGAAACACCG GGAAGATACT 
GTCTTCTCTG ATGTTATGGA AGGGGAAGAA AACACAGACA CAGATTTAGA GAGTTCTGAT 
TCAAAGGAAG AGGATGACCC ATTAGTCATG GATAGCAGAT TGGGGAAACC ACGACCAGAA 
GATCATACTG ACCCTGAAAA GGCAGCAGAC CACCTTGTGA ATGTAGAAGT CCCCAAAGCA 
GACAGTGACG ACGACCCGGA AGTGGGCGCA GGACTTCACA TGAAAGACAA GGGGCGGAAA 
GTTGAGGAGC CCAGGAGGGA CTGGGTGCAG CATGAGGTAG GGCTAGAGGA CGAGACGCAA 
GAAGACCAGG CTGTGCAGGG CTCTTCTCAG AGTGGCCACC TCAGGTCTTC ACCAGCTGCT 
GAAAAAAGTA CAGAAACATT AAAATCAGCC TTTGCTAACC AAGAGAATGA CCTGAAAGGA 
GCAGCCGTTC ACATCTCCAA AGAAATGCTT CACGAAGAAA AGCCCTCAGG GCGGAGTTTG 
GAAGGTGCCT CAAAGAGTGA CTCAGTCCCG CAAGCAAAAG CGGCGGGGAA TCAAGGGGAG 
GAAGGAAAGA CTGAACGGGA ACCTGTGGGC GTGGCAGCCC CCTCGGGCGA TCATCAGCCT 
AATGCTTCCA AAGACAGCGT GGACGAAGTA GACGGTTCAA TAAGTGGGCC TAAACCACAC 
GTGCTTTCGG GGGAGCATCC GAGCGCAGAA CTTATAAAGG ATCGGCTTCT TAAACTTCAA 
AATCAGACCA GGTTCTCCTC TCCAGATGAC ATGGGCTTGC CAGGAGACCT GGAAAAAAAG 
CGGCCCATTC TGGAAAGAAA GCTTTCCTGG CAACAAGGAG GTGTGGCTGC CGCAGTTAAT 
AAGCAAGTGA GCGAAAAGAG AGAGCTCCCT GAAGAAGAGG TCACCAGGGT CACCAAGGAT 
GCCTCAGATG AAGGGCAGGA AGTAAGAAAA ACAGGCCAAA CTGATAGCAT AGAAGGGCGA 
GGCTTCCGCC CAAAAGAACC AAACCCAGAA GATGAAGATT ACAGCCCTGA AGAACTCCTG 
GAGGATGAAA ATGCTGTAAG TGCAAAGCAG TCTAAAGAAA GAAGCCCTGA GATTCAGGAT 
AAGAGGTTAG ATGTTGACCT GCAAAACCCT GAAAAACCTG TTTCAGGTGC CATTAAGACT 
GATCCAGAAA CTGAAAAAAA CAAAGAAGAA ACTAGGCATG TATCGGAAAA TGAAAGAAAA 
AATGAGACTG CGGGTAAAGC GGTAGACAGT CTGGGCAGGG ACGCAGGTGG CCCGGTGGTG 
GAAAAAGAAG GAAGCTCTCC TGTGCATCAG AAGGTACAGA GACCCTCTGA AGGAAGTGAC 
GTTCCTGGCA AGAAACAAAA TCAGACTCCA GAGTTAGGGG AAGCGTCTCA GAAGAAAGAT 
CCTGATTATC TTAAAGAAGA CAACCATGAG GGCCACCCAA AGACCTCAGG GCTCATGGAG 
AAGCCTGGGG TAGAACCCTC GAAAGAGGAC GACGAGCATG CAGAGAAATT CGTGGACCCA 
GGAAGCCGGG GGTCTGCTTC CGAGGACCCT GACGATGACC CGTTCCCCTG GGCTCCACAT 
GCCCCTGTGC AGCCTGAGGA GAGTGTCCAT TTGGAGGACT TGCCCATAAT CAGCAGCTTC 
TTTAAAGATC AGCAGTCTCT GCAGCGATTC CAGAAGTACT TTGACATCCA CAAGCTGGAA 
GCCATGTTCC AGGACATGTC ATCCAAGCTG AAGTCAGCTC AGCGTGAGAG CCTGCCCTAT 
AACGTGGAGA AAGTCCTCGA TAAGGTCTTT CGTGCTTGGG AGTCACACAT TCTGACAGAG 
GCAGAGAACA TGCTTGATGC TCGCGTGACG GAAAACAGAG ACCTGGAAAC GAAGGATAGT 
AGCGTCTTTG AGGAGGCTGC TGTGCTCGAT GATGTTCAAG ACCTGATCTA TTTCGTCAGG 
TACAAACACT CCACAGTGGA GGAGACTGCT CCCCCAGCAG CGGCACAGCC TGTAGAGGGA 
GGCTGGGATG GACCAGCAGA AGACACACAG CCACCACTGG AGGAGAACTT CCCACAGGAG 
CACATGGAAG TTCCTCTGAT GCAGATTCCC AAAGAGCCCG GCCATTTGGC TCAACCCGTG 
ACCAGGGACA TGGGTACCTC AGGAGTGGCC CAGAAGCCGC AAACCGAGGA AGATGGAGAC 
CCAGGGATAA TTACACCACA AGGCACTCCT GTCGATGCTG ATGATGCCCA AAAGCAGCTG 
GCAGCAAATA CTGAAGAACC AGCAAGTGTC ACGCCTCTGG AAAATGCAAT TGCTTTCATA 
TACTCACTTG TGTTTCATTT AACTAAGACG CTCCTTGCTA CATTGCCTGA TGATGTCCAG 
CCTGGGCCTG ATTTTTATGG ACTGCCATGG AAGCCTGTAC TTATCACTGC CTCCTTAGGA 
ATTGTTTCAT TTGCCGTTTT CTTCTGGAGA ACTGTCCTTG CTGTAAAGAG TAGAGTATAT 
CAAGTCACTG AACAGCAAAT TTCTGAGAAG TTGAAGAATA TCATGAAAGA AAACGCAGAA 
CTTGTACAAA AATTGTCAAG TTATGAGCAG AAGATCAAGG AATCAAAGAA ACATGTTCAA 
GAAACCAAGA AACAAAACAT GATTCTTTCT GATGAAGCAA TTAAATTTAA GGATAAAATC 
AAGAGTCTTG AAGAAACTAA TGAAATTCTG GGAGACACAG CTAAAAGTTT ACGCGCTATG 
TTAGAGTCTG AGAGAGAACA GAATGCCAAG AATCAGGACT TGATATCAGA AAATAAGAAA 
TCTATAGAGA AGTTAAAGGA TGTTATCTCA GTGAATGCCT CAGAATTTTC AGAGGTTCAA 
ATTGCCCTTA ATGAAGCTAA ACTTAGTGAA GAGAAAGTGA AGTCTGAATG CCATCGGGTT 
CAAGAAGAAA ATGCTAGACT TAAGAAGAAG AAAGAGCAGT TGCAGCAAGA AATCAAAGAC 
TGGAGTAAAT CACATGCTGA GCTCAGTGAG CAAATCAGAT CATTTGAGAA GTCTCAGAAA 
GATCTAGAAG TAGCTCTCAC TCACAAAGAT GATAATATTA ATGCTTTGAC TAATTGCATT 
ACACAGTTGA ATCGGTTAGA TTGTGAATCT GAATCTGAGG ATCAAAATAA AGGAGGAAGT 
GAATCAGATG AATTAGCAAA TGGAGAAGTG GGAGGTGACC GGAGTGAGAA GGTGAAAAAT 
CAGATTAAGC AGATGATGGA CGTTTCTCGG ACACAGACTG CAATATCAGT AGTTGAAGAG 
GATCTGAAAC TTTTACAGTG TAAACTCAGA GCCTCCATGT CCACTAAGTG TAACCTGGAA 
GACCAAATAA AGAAATTAGA AGAGGATCGC AGCTCACTTC AGTCTGCCAA AACTGTGCTG 
GAAGATGAAT GCAAAACACT GAGGCAGAAA GTAGAGATTC TAAATGAACT CTATCAGCAG 
AAGGAGATGG CTCTACAAAA GAAATTGAGT CAAGAAGAGT ATGAGCGGCA AGAAAGAGAG 
CAGCGGCTGT CAGCTGCAGA TGAAAAGGCG GTTTTGGCGG CAGAGGAAGT GAAAACTTAC 
AAGCGGAGAA TTGAAGAAAT GGAGGATGAA CTACAGAAAA CAGAGCGCTC ATTTAAAAAC 
CAGATTGCTA CTCATGAGAA GAAAGCTCAC GATAACTGGC TCAAAGCTCG TGCTGCAGAA 
AGAGCTATAG CTGAAGAGAA GAGAGAAGCT GCCAACTTGA GACATAAATT ATTGGAACTA 
ACCCAAAAGA TGGCAATGAT GCAAGAAGAA CCTGTGATTG TAAAACCAAT GCCAGGAAGA 
CCCAACACAC AAAACCCTCC ACGGAGAGGT CCCCTGAGCC AGAATGGCTC TTTTGGCCCA 
TCTCCTGTGA GCGGTGGAGA ATGCTCCCCA CCGCTGACGG CAGACCCGCC CGCAAGACCT 
CTGTCTGCTA CGCTTAATCG AAGAGAGATG CCTCGAAGTG AATTTGGATC AGTGGATGGG 
CCTCTCCCTC GACCTCGATG GGCCTCTGAG GCATCTGGGA AACCCTCTGC CTCTGATCCA 
GAATCCGGCG CTGCTCCCAC AGTGAACAGC AGCTCCAGAA GCTCTTCGCC CTCTAAGGTG 
ATGGATGAGG GCAAGCAAAC TGTTCCCCAA GAGCCTGAAG GCCCTTCAGT TCCCAGCATT 
CCTTCTTTGG CTGAGCATCC AGTATCAGTT AGTATGGCTG CAAAAGGGCC CCCTCCCTTT 
CCAGGGACAC CCCTCATGAG CTCCCCTGTG GGAGGCCCTC TACTGCCACC CATCCGATAT 
GGACCACCTC CTCAACTCTG TGGGCCTTTC GGACCTCGGC CACTCCCTCC ACCCTTTGGT 
CCTGGTATGC GTCCACCGCT AGGCTTAAGA GAATATGCAC CAGGTGTTCC ACCGGGAAAA 
CGGGACCTGC CTCTCGACCC TCGAGAATTT TTACCACCAG GACATGCACC TTTTAGACCT 
TTAGGCTCTC TTGGCCCAAG AGAGTACTTT TTTCCTGGTA CCCGATTACC ACCCCCCAAT 
CATGGGCCCC AGGATTATCC ACCTTCCTCT GCTGCAAGAG ACTTACCGCC CTCCGGCTCC 
AGAGATGAGC CCCCGCCTGC CTCTCAGGGC GCTAGCCAGG ACTGCTCACC GGCTTTAAAA 
CAGAGCCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831922.1
CDS	238..5889
Translation	MYRGEALEDFTGPDCRFVNFKKGDTVYVYYKLAGGSPEVWAGSVGHTFGYFPKDLIQVVHEYTQEELQVPTDETDFVCFDGGRDDFDNYNVEDLLGFLELYDSATEDSEKVKEKTAQRVEEPPEASNESDAEPEPGEPNSEESESVLSENTAELRERSEAQKSHPQVNSQTGHAQGERTSFESFGEMLQDKLKVPDSENNKTSNSSQVSHEQEKIDAYKLLKTEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFVEDLDPEYYTVGKEEEENKEDFDELPLLTFTDGEDTKSPGHSGIEKHPTEKEQNSNKEHKVEETQPPGIKKGDKEIPKHREDTVFSDVMEGEENTDTDLESSDSKEEDDPLVMDSRLGKPRPEDHTDPEKAADHLVNVEVPKADSDDDPEVGAGLHMKDKGRKVEEPRRDWVQHEVGLEDETQEDQAVQGSSQSGHLRSSPAAEKSTETLKSAFANQENDLKGAAVHISKEMLHEEKPSGRSLEGASKSDSVPQAKAAGNQGEEGKTEREPVGVAAPSGDHQPNASKDSVDEVDGSISGPKPHVLSGEHPSAELIKDRLLKLQNQTRFSSPDDMGLPGDLEKKRPILERKLSWQQGGVAAAVNKQVSEKRELPEEEVTRVTKDASDEGQEVRKTGQTDSIEGRGFRPKEPNPEDEDYSPEELLEDENAVSAKQSKERSPEIQDKRLDVDLQNPEKPVSGAIKTDPETEKNKEETRHVSENERKNETAGKAVDSLGRDAGGPVVEKEGSSPVHQKVQRPSEGSDVPGKKQNQTPELGEASQKKDPDYLKEDNHEGHPKTSGLMEKPGVEPSKEDDEHAEKFVDPGSRGSASEDPDDDPFPWAPHAPVQPEESVHLEDLPIISSFFKDQQSLQRFQKYFDIHKLEAMFQDMSSKLKSAQRESLPYNVEKVLDKVFRAWESHILTEAENMLDARVTENRDLETKDSSVFEEAAVLDDVQDLIYFVRYKHSTVEETAPPAAAQPVEGGWDGPAEDTQPPLEENFPQEHMEVPLMQIPKEPGHLAQPVTRDMGTSGVAQKPQTEEDGDPGIITPQGTPVDADDAQKQLAANTEEPASVTPLENAIAFIYSLVFHLTKTLLATLPDDVQPGPDFYGLPWKPVLITASLGIVSFAVFFWRTVLAVKSRVYQVTEQQISEKLKNIMKENAELVQKLSSYEQKIKESKKHVQETKKQNMILSDEAIKFKDKIKSLEETNEILGDTAKSLRAMLESEREQNAKNQDLISENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIRSFEKSQKDLEVALTHKDDNINALTNCITQLNRLDCESESEDQNKGGSESDELANGEVGGDRSEKVKNQIKQMMDVSRTQTAISVVEEDLKLLQCKLRASMSTKCNLEDQIKKLEEDRSSLQSAKTVLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKAVLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMMQEEPVIVKPMPGRPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTADPPARPLSATLNRREMPRSEFGSVDGPLPRPRWASEASGKPSASDPESGAAPTVNSSSRSSSPSKVMDEGKQTVPQEPEGPSVPSIPSLAEHPVSVSMAAKGPPPFPGTPLMSSPVGGPLLPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREYAPGVPPGKRDLPLDPREFLPPGHAPFRPLGSLGPREYFFPGTRLPPPNHGPQDYPPSSAARDLPPSGSRDEPPPASQGASQDCSPALKQSP

Target ORF information:

RefSeq Version	XM_024976154.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976154.1

ORF Insert Sequence:

ATGTACCGGG GTGAGGCTCT AGAAGATTTT ACAGGCCCGG ATTGTCGTTT TGTGAATTTT 
AAAAAAGGAG ACACTGTATA TGTCTACTAT AAACTGGCAG GGGGATCACC TGAAGTTTGG 
GCTGGAAGTG TTGGCCATAC TTTTGGATAT TTTCCAAAGG ATTTAATCCA AGTAGTCCAT 
GAATATACTC AGGAAGAGCT GCAAGTTCCA ACAGATGAGA CAGATTTCGT TTGTTTTGAT 
GGGGGAAGAG ATGATTTTGA TAATTATAAT GTCGAAGACC TTTTAGGATT TTTGGAATTG 
TACGATTCTG CCACTGAAGA TTCTGAAAAA GTTAAAGAAA AAACTGCTCA GCGTGTGGAA 
GAACCTCCTG AAGCATCTAA TGAAAGTGAC GCTGAACCTG AGCCCGGAGA ACCCAACTCA 
GAGGAAAGTG AAAGCGTACT CTCAGAAAAT ACTGCGGAAC TCAGGGAAAG ATCTGAGGCT 
CAGAAGAGCC ACCCCCAGGT AAACAGTCAA ACAGGTCATG CTCAGGGAGA GCGAACTTCA 
TTTGAATCTT TTGGAGAAAT GCTGCAAGAT AAGTTAAAAG TGCCAGACAG TGAAAACAAC 
AAAACCAGCA ATAGTTCTCA GGTCTCGCAC GAGCAGGAGA AGATCGATGC TTATAAACTT 
TTGAAAACAG AAATGACTCT GGACTTGAAA ACCAAATTTG GCTCAACTGC AGATGCACTG 
GTATCTGATG ATGAGACAAC CAGGCTTGTT ACTTCATTAG AGGACGATTT TGTTGAGGAT 
TTGGATCCTG AGTATTACAC AGTGGGAAAG GAAGAAGAGG AGAATAAAGA AGACTTCGAT 
GAGCTGCCGT TACTAACCTT TACAGATGGA GAAGATACGA AAAGTCCAGG GCACTCCGGA 
ATTGAGAAAC ACCCAACAGA GAAAGAGCAG AATTCAAACA AAGAGCATAA AGTTGAGGAA 
ACTCAGCCCC CAGGCATCAA AAAGGGTGAT AAAGAGATAC CGAAACACCG GGAAGATACT 
GTCTTCTCTG ATGTTATGGA AGGGGAAGAA AACACAGACA CAGATTTAGA GAGTTCTGAT 
TCAAAGGAAG AGGATGACCC ATTAGTCATG GATAGCAGAT TGGGGAAACC ACGACCAGAA 
GATCATACTG ACCCTGAAAA GGCAGCAGAC CACCTTGTGA ATGTAGAAGT CCCCAAAGCA 
GACAGTGACG ACGACCCGGA AGTGGGCGCA GGACTTCACA TGAAAGACAA GGGGCGGAAA 
GTTGAGGAGC CCAGGAGGGA CTGGGTGCAG CATGAGGTAG GGCTAGAGGA CGAGACGCAA 
GAAGACCAGG CTGTGCAGGG CTCTTCTCAG AGTGGCCACC TCAGGTCTTC ACCAGCTGCT 
GAAAAAAGTA CAGAAACATT AAAATCAGCC TTTGCTAACC AAGAGAATGA CCTGAAAGGA 
GCAGCCGTTC ACATCTCCAA AGAAATGCTT CACGAAGAAA AGCCCTCAGG GCGGAGTTTG 
GAAGGTGCCT CAAAGAGTGA CTCAGTCCCG CAAGCAAAAG CGGCGGGGAA TCAAGGGGAG 
GAAGGAAAGA CTGAACGGGA ACCTGTGGGC GTGGCAGCCC CCTCGGGCGA TCATCAGCCT 
AATGCTTCCA AAGACAGCGT GGACGAAGTA GACGGTTCAA TAAGTGGGCC TAAACCACAC 
GTGCTTTCGG GGGAGCATCC GAGCGCAGAA CTTATAAAGG ATCGGCTTCT TAAACTTCAA 
AATCAGACCA GGTTCTCCTC TCCAGATGAC ATGGGCTTGC CAGGAGACCT GGAAAAAAAG 
CGGCCCATTC TGGAAAGAAA GCTTTCCTGG CAACAAGGAG GTGTGGCTGC CGCAGTTAAT 
AAGCAAGTGA GCGAAAAGAG AGAGCTCCCT GAAGAAGAGG TCACCAGGGT CACCAAGGAT 
GCCTCAGATG AAGGGCAGGA AGTAAGAAAA ACAGGCCAAA CTGATAGCAT AGAAGGGCGA 
GGCTTCCGCC CAAAAGAACC AAACCCAGAA GATGAAGATT ACAGCCCTGA AGAACTCCTG 
GAGGATGAAA ATGCTGTAAG TGCAAAGCAG TCTAAAGAAA GAAGCCCTGA GATTCAGGAT 
AAGAGGTTAG ATGTTGACCT GCAAAACCCT GAAAAACCTG TTTCAGGTGC CATTAAGACT 
GATCCAGAAA CTGAAAAAAA CAAAGAAGAA ACTAGGCATG TATCGGAAAA TGAAAGAAAA 
AATGAGACTG CGGGTAAAGC GGTAGACAGT CTGGGCAGGG ACGCAGGTGG CCCGGTGGTG 
GAAAAAGAAG GAAGCTCTCC TGTGCATCAG AAGGTACAGA GACCCTCTGA AGGAAGTGAC 
GTTCCTGGCA AGAAACAAAA TCAGACTCCA GAGTTAGGGG AAGCGTCTCA GAAGAAAGAT 
CCTGATTATC TTAAAGAAGA CAACCATGAG GGCCACCCAA AGACCTCAGG GCTCATGGAG 
AAGCCTGGGG TAGAACCCTC GAAAGAGGAC GACGAGCATG CAGAGAAATT CGTGGACCCA 
GGAAGCCGGG GGTCTGCTTC CGAGGACCCT GACGATGACC CGTTCCCCTG GGCTCCACAT 
GCCCCTGTGC AGCCTGAGGA GAGTGTCCAT TTGGAGGACT TGCCCATAAT CAGCAGCTTC 
TTTAAAGATC AGCAGTCTCT GCAGCGATTC CAGAAGTACT TTGACATCCA CAAGCTGGAA 
GCCATGTTCC AGGACATGTC ATCCAAGCTG AAGTCAGCTC AGCGTGAGAG CCTGCCCTAT 
AACGTGGAGA AAGTCCTCGA TAAGGTCTTT CGTGCTTGGG AGTCACACAT TCTGACAGAG 
GCAGAGAACA TGCTTGATGC TCGCGTGACG GAAAACAGAG ACCTGGAAAC GAAGGATAGT 
AGCGTCTTTG AGGAGGCTGC TGTGCTCGAT GATGTTCAAG ACCTGATCTA TTTCGTCAGG 
TACAAACACT CCACAGTGGA GGAGACTGCT CCCCCAGCAG CGGCACAGCC TGTAGAGGGA 
GGCTGGGATG GACCAGCAGA AGACACACAG CCACCACTGG AGGAGAACTT CCCACAGGAG 
CACATGGAAG TTCCTCTGAT GCAGATTCCC AAAGAGCCCG GCCATTTGGC TCAACCCGTG 
ACCAGGGACA TGGGTACCTC AGGAGTGGCC CAGAAGCCGC AAACCGAGGA AGATGGAGAC 
CCAGGGATAA TTACACCACA AGGCACTCCT GTCGATGCTG ATGATGCCCA AAAGCAGCTG 
GCAGCAAATA CTGAAGAACC AGCAAGTGTC ACGCCTCTGG AAAATGCAAT TGCTTTCATA 
TACTCACTTG TGTTTCATTT AACTAAGACG CTCCTTGCTA CATTGCCTGA TGATGTCCAG 
CCTGGGCCTG ATTTTTATGG ACTGCCATGG AAGCCTGTAC TTATCACTGC CTCCTTAGGA 
ATTGTTTCAT TTGCCGTTTT CTTCTGGAGA ACTGTCCTTG CTGTAAAGAG TAGAGTATAT 
CAAGTCACTG AACAGCAAAT TTCTGAGAAG TTGAAGAATA TCATGAAAGA AAACGCAGAA 
CTTGTACAAA AATTGTCAAG TTATGAGCAG AAGATCAAGG AATCAAAGAA ACATGTTCAA 
GAAACCAAGA AACAAAACAT GATTCTTTCT GATGAAGCAA TTAAATTTAA GGATAAAATC 
AAGAGTCTTG AAGAAACTAA TGAAATTCTG GGAGACACAG CTAAAAGTTT ACGCGCTATG 
TTAGAGTCTG AGAGAGAACA GAATGCCAAG AATCAGGACT TGATATCAGA AAATAAGAAA 
TCTATAGAGA AGTTAAAGGA TGTTATCTCA GTGAATGCCT CAGAATTTTC AGAGGTTCAA 
ATTGCCCTTA ATGAAGCTAA ACTTAGTGAA GAGAAAGTGA AGTCTGAATG CCATCGGGTT 
CAAGAAGAAA ATGCTAGACT TAAGAAGAAG AAAGAGCAGT TGCAGCAAGA AATCAAAGAC 
TGGAGTAAAT CACATGCTGA GCTCAGTGAG CAAATCAGAT CATTTGAGAA GTCTCAGAAA 
GATCTAGAAG TAGCTCTCAC TCACAAAGAT GATAATATTA ATGCTTTGAC TAATTGCATT 
ACACAGTTGA ATCGGTTAGA TTGTGAATCT GAATCTGAGG ATCAAAATAA AGGAGGAAGT 
GAATCAGATG AATTAGCAAA TGGAGAAGTG GGAGGTGACC GGAGTGAGAA GGTGAAAAAT 
CAGATTAAGC AGATGATGGA CGTTTCTCGG ACACAGACTG CAATATCAGT AGTTGAAGAG 
GATCTGAAAC TTTTACAGTG TAAACTCAGA GCCTCCATGT CCACTAAGTG TAACCTGGAA 
GACCAAATAA AGAAATTAGA AGAGGATCGC AGCTCACTTC AGTCTGCCAA AACTGTGCTG 
GAAGATGAAT GCAAAACACT GAGGCAGAAA GTAGAGATTC TAAATGAACT CTATCAGCAG 
AAGGAGATGG CTCTACAAAA GAAATTGAGT CAAGAAGAGT ATGAGCGGCA AGAAAGAGAG 
CAGCGGCTGT CAGCTGCAGA TGAAAAGGCG GTTTTGGCGG CAGAGGAAGT GAAAACTTAC 
AAGCGGAGAA TTGAAGAAAT GGAGGATGAA CTACAGAAAA CAGAGCGCTC ATTTAAAAAC 
CAGATTGCTA CTCATGAGAA GAAAGCTCAC GATAACTGGC TCAAAGCTCG TGCTGCAGAA 
AGAGCTATAG CTGAAGAGAA GAGAGAAGCT GCCAACTTGA GACATAAATT ATTGGAACTA 
ACCCAAAAGA TGGCAATGAT GCAAGAAGAA CCTGTGATTG TAAAACCAAT GCCAGGAAGA 
CCCAACACAC AAAACCCTCC ACGGAGAGGT CCCCTGAGCC AGAATGGCTC TTTTGGCCCA 
TCTCCTGTGA GCGGTGGAGA ATGCTCCCCA CCGCTGACGG CAGACCCGCC CGCAAGACCT 
CTGTCTGCTA CGCTTAATCG AAGAGAGATG CCTCGAAGTG AATTTGGATC AGTGGATGGG 
CCTCTCCCTC GACCTCGATG GGCCTCTGAG GCATCTGGGA AACCCTCTGC CTCTGATCCA 
GAATCCGGCG CTGCTCCCAC AGTGAACAGC AGCTCCAGAA GCTCTTCGCC CTCTAAGGTG 
ATGGATGAGG GCAAGCAAAC TGTTCCCCAA GAGCCTGAAG GCCCTTCAGT TCCCAGCATT 
CCTTCTTTGG CTGAGCATCC AGTATCAGTT AGTATGGCTG CAAAAGGGCC CCCTCCCTTT 
CCAGGGACAC CCCTCATGAG CTCCCCTGTG GGAGGCCCTC TACTGCCACC CATCCGATAT 
GGACCACCTC CTCAACTCTG TGGGCCTTTC GGACCTCGGC CACTCCCTCC ACCCTTTGGT 
CCTGGTATGC GTCCACCGCT AGGCTTAAGA GAATATGCAC CAGGTGTTCC ACCGGGAAAA 
CGGGACCTGC CTCTCGACCC TCGAGAATTT TTACCACCAG GACATGCACC TTTTAGACCT 
TTAGGCTCTC TTGGCCCAAG AGAGTACTTT TTTCCTGGTA CCCGATTACC ACCCCCCAAT 
CATGGGCCCC AGGATTATCC ACCTTCCTCT GCTGCAAGAG ACTTACCGCC CTCCGGCTCC 
AGAGATGAGC CCCCGCCTGC CTCTCAGGGC GCTAGCCAGG ACTGCTCACC GGCTTTAAAA 
CAGAGCCCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_024976155.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831923.1 Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X4, mRNA.

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CloneID	OBa223512
Clone ID Related Accession (Same CDS sequence)	XM_024976155.1
Accession Version	XM_024976155.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5649bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	transport and Golgi organization protein 1 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037343.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCGG CGCAGGGGCT GCTCTTCTGG CTGCTCCTGC TCGGGCCGCC TTGGCGCGTG 
CCCGGCCAGC CGGAGCAGGA CCCCGGCCGG CGCTTCTCGC AGCTCAAACT CTGCGCGGAC 
GAAGAATGCA GCATGCTAAT GTACCGGGGT GAGGCTCTAG AAGATTTTAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGAGAC ACTGTATATG TCTACTATAA ACTGGCAGGG 
GGATCACCTG AAGTTTGGGC TGGAAGTGTT GGCCATACTT TTGGATATTT TCCAAAGGAT 
TTAATCCAAG TAGTCCATGA ATATACTCAG GAAGAGCTGC AAGTTCCAAC AGATGAGACA 
GATTTCGTTT GTTTTGATGG GGGAAGAGAT GATTTTGATA ATTATAATGT CGAAGACCTT 
TTAGGATTTT TGGAATTGTA CGATTCTGCC ACTGAAGATT CTGAAAAAGT TAAAGAAAAA 
ACTGCTCAGC GTGTGGAAGA ACCTCCTGAA GCATCTAATG AAAGTGACGC TGAACCTGAG 
CCCGGAGAAC CCAACTCAGA GGAAAGTGAA AGCGTACTCT CAGAAAATAC TGCGGAACTC 
AGGGAAAGAT CTGAGGCTCA GAAGAGCCAC CCCCAGGTAA ACAGTCAAAC AGGTCATGCT 
CAGGGAGAGC GAACTTCATT TGAATCTTTT GGAGAAATGC TGCAAGATAA GTTAAAAGTG 
CCAGACAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCGCACGA GCAGGAGAAG 
ATCGATGCTT ATAAACTTTT GAAAACAGAA ATGACTCTGG ACTTGAAAAC CAAATTTGGC 
TCAACTGCAG ATGCACTGGT ATCTGATGAT GAGACAACCA GGCTTGTTAC TTCATTAGAG 
GACGATTTTG TTGAGGATTT GGATCCTGAG TATTACACAG TGGGAAAGGA AGAAGAGGAG 
AATAAAGAAG ACTTCGATGA GCTGCCGTTA CTAACCTTTA CAGATGGAGA AGATACGAAA 
AGTCCAGGGC ACTCCGGAAT TGAGAAACAC CCAACAGAGA AAGAGCAGAA TTCAAACAAA 
GAGCATAAAG TTGAGGAAAC TCAGCCCCCA GGCATCAAAA AGGGTGATAA AGAGATACCG 
AAACACCGGG AAGATACTGT CTTCTCTGAT GTTATGGAAG GGGAAGAAAA CACAGACACA 
GATTTAGAGA GTTCTGATTC AAAGGAAGAG GATGACCCAT TAGTCATGGA TAGCAGATTG 
GGGAAACCAC GACCAGAAGA TCATACTGAC CCTGAAAAGG CAGCAGACCA CCTTGTGAAT 
GTAGAAGTCC CCAAAGCAGA CAGTGACGAC GACCCGGAAG TGGGCGCAGG ACTTCACATG 
AAAGACAAGG GGCGGAAAGT TGAGGAGCCC AGGAGGGACT GGGTGCAGCA TGAGGTAGGG 
CTAGAGGACG AGACGCAAGA AGACCAGGCT GTGCAGGGCT CTTCTCAGAG TGGCCACCTC 
AGGTCTTCAC CAGCTGCTGA AAAAAGTACA GAAACATTAA AATCAGCCTT TGCTAACCAA 
GAGAATGACC TGAAAGGAGC AGCCGTTCAC ATCTCCAAAG AAATGCTTCA CGAAGAAAAG 
CCCTCAGGGC GGAGTTTGGA AGGTGCCTCA AAGAGTGACT CAGTCCCGCA AGCAAAAGCG 
GCGGGGAATC AAGGGGAGGA AGGAAAGACT GAACGGGAAC CTGTGGGCGT GGCAGCCCCC 
TCGGGCGATC ATCAGCCTAA TGCTTCCAAA GACAGCGTGG ACGAAGTAGA CGGTTCAATA 
AGTGGGCCTA AACCACACGT GCTTTCGGGG GAGCATCCGA GCGCAGAACT TATAAAGGAT 
CGGCTTCTTA AACTTCAAAA TCAGACCAGG TTCTCCTCTC CAGATGACAT GGGCTTGCCA 
GGAGACCTGG AAAAAAAGCG GCCCATTCTG GAAAGAAAGC TTTCCTGGCA ACAAGGAGGT 
GTGGCTGCCG CAGTTAATAA GCAAGTGAGC GAAAAGAGAG AGCTCCCTGA AGAAGAGGTC 
ACCAGGGTCA CCAAGGATGC CTCAGATGAA GGGCAGGAAG TAAGAAAAAC AGGCCAAACT 
GATAGCATAG AAGGGCGAGG CTTCCGCCCA AAAGAACCAA ACCCAGAAGA TGAAGATTAC 
AGCCCTGAAG AACTCCTGGA GGATGAAAAT GCTGTAAGTG CAAAGCAGTC TAAAGAAAGA 
AGCCCTGAGA TTCAGGATAA GAGGTTAGAT GTTGACCTGC AAAACCCTGA AAAACCTGTT 
TCAGGTGCCA TTAAGACTGA TCCAGAAACT GAAAAAAACA AAGAAGAAAC TAGGCATGTA 
TCGGAAAATG AAAGAAAAAA TGAGACTGCG GGTAAAGCGG TAGACAGTCT GGGCAGGGAC 
GCAGGTGGCC CGGTGGTGGA AAAAGAAGGA AGCTCTCCTG TGCATCAGAA GGTACAGAGA 
CCCTCTGAAG GAAGTGACGT TCCTGGCAAG AAACAAAATC AGACTCCAGA GTTAGGGGAA 
GCGTCTCAGA AGAAAGATCC TGATTATCTT AAAGAAGACA ACCATGAGGG CCACCCAAAG 
ACCTCAGGGC TCATGGAGAA GCCTGGGGTA GAACCCTCGA AAGAGGACGA CGAGCATGCA 
GAGAAATTCG TGGACCCAGG AAGCCGGGGG TCTGCTTCCG AGGACCCTGA CGATGACCCG 
TTCCCCTGGG CTCCACATGC CCCTGTGCAG CCTGAGGAGA GTGTCCATTT GGAGGACTTG 
CCCATAATCA GCAGCTTCTT TAAAGATCAG CAGTCTCTGC AGCGATTCCA GAAGTACTTT 
GACATCCACA AGCTGGAAGC CATGTTCCAG GACATGTCAT CCAAGCTGAA GTCAGCTCAG 
CGTGAGAGCC TGCCCTATAA CGTGGAGAAA GTCCTCGATA AGGTCTTTCG TGCTTGGGAG 
TCACACATTC TGACAGAGGC AGAGAACATG CTTGATGCTC GCGTGACGGA AAACAGAGAC 
CTGGAAACGA AGGATAGTAG CGTCTTTGAG GAGGCTGCTG TGCTCGATGA TGTTCAAGAC 
CTGATCTATT TCGTCAGGTA CAAACACTCC ACAGTGGAGG AGACTGCTCC CCCAGCAGCG 
GCACAGCCTG TAGAGGGAGG CTGGGATGGA CCAGCAGAAG ACACACAGCC ACCACTGGAG 
GAGAACTTCC CACAGGAGCA CATGGAAGTT CCTCTGATGC AGATTCCCAA AGAGCCCGGC 
CATTTGGCTC AACCCGTGAC CAGGGACATG GGTACCTCAG GAGTGGCCCA GAAGCCGCAA 
ACCGAGGAAG ATGGAGACCC AGGGATAATT ACACCACAAG GCACTCCTGT CGATGCTGAT 
GATGCCCAAA AGCAGCTGGC AGCAAATACT GAAGAACCAG CAAGTGTCAC GCCTCTGGAA 
AATGCAATTG CTTTCATATA CTCACTTGTG TTTCATTTAA CTAAGACGCT CCTTGCTACA 
TTGCCTGATG ATGTCCAGCC TGGGCCTGAT TTTTATGGAC TGCCATGGAA GCCTGTACTT 
ATCACTGCCT CCTTAGGAAT TGTTTCATTT GCCGTTTTCT TCTGGAGAAC TGTCCTTGCT 
GTAAAGAGTA GAGTATATCA AGTCACTGAA CAGCAAATTT CTGAGAAGTT GAAGAATATC 
ATGAAAGAAA ACGCAGAACT TGTACAAAAA TTGTCAAGTT ATGAGCAGAA GATCAAGGAA 
TCAAAGAAAC ATGTTCAAGA AACCAAGAAA CAAAACATGA TTCTTTCTGA TGAAGCAATT 
AAATTTAAGG ATAAAATCAA GAGTCTTGAA GAAACTAATG AAATTCTGGG AGACACAGCT 
AAAAGTTTAC GCGCTATGTT AGAGTCTGAG AGAGAACAGA ATGCCAAGAA TCAGGACTTG 
ATATCAGAAA ATAAGAAATC TATAGAGAAG TTAAAGGATG TTATCTCAGT GAATGCCTCA 
GAATTTTCAG AGGTTCAAAT TGCCCTTAAT GAAGCTAAAC TTAGTGAAGA GAAAGTGAAG 
TCTGAATGCC ATCGGGTTCA AGAAGAAAAT GCTAGACTTA AGAAGAAGAA AGAGCAGTTG 
CAGCAAGAAA TCAAAGACTG GAGTAAATCA CATGCTGAGC TCAGTGAGCA AATCAGATCA 
TTTGAGAAGT CTCAGAAAGA TCTAGAAGTA GCTCTCACTC ACAAAGATGA TAATATTAAT 
GCTTTGACTA ATTGCATTAC ACAGTTGAAT CGGTTAGATT GTGAATCTGA ATCTGAGGAT 
CAAAATAAAG GAGGAAGTGA ATCAGATGAA TTAGCAAATG GAGAAGTGGG AGGTGACCGG 
AGTGAGAAGG TGAAAAATCA GATTAAGCAG ATGATGGACG TTTCTCGGAC ACAGACTGCA 
ATATCAGTAG TTGAAGAGGA TCTGAAACTT TTACAGTGTA AACTCAGAGC CTCCATGTCC 
ACTAAGTGTA ACCTGGAAGA CCAAATAAAG AAATTAGAAG AGGATCGCAG CTCACTTCAG 
TCTGCCAAAA CTGTGCTGGA AGATGAATGC AAAACACTGA GGCAGAAAGT AGAGATTCTA 
AATGAACTCT ATCAGCAGAA GGAGATGGCT CTACAAAAGA AATTGAGTCA AGAAGAGTAT 
GAGCGGCAAG AAAGAGAGCA GCGGCTGTCA GCTGCAGATG AAAAGGCGGT TTTGGCGGCA 
GAGGAAGTGA AAACTTACAA GCGGAGAATT GAAGAAATGG AGGATGAACT ACAGAAAACA 
GAGCGCTCAT TTAAAAACCA GATTGCTACT CATGAGAAGA AAGCTCACGA TAACTGGCTC 
AAAGCTCGTG CTGCAGAAAG AGCTATAGCT GAAGAGAAGA GAGAAGCTGC CAACTTGAGA 
CATAAATTAT TGGAACTAAC CCAAAAGATG GCAATGATGC AAGAAGAACC TGTGATTGTA 
AAACCAATGC CAGGAAGACC CAACACACAA AACCCTCCAC GGAGAGGTCC CCTGAGCCAG 
AATGGCTCTT TTGGCCCATC TCCTGTGAGC GGTGGAGAAT GCTCCCCACC GCTGACGGCA 
GACCCGCCCG CAAGACCTCT GTCTGCTACG CTTAATCGAA GAGAGATGCC TCGAAGTGAA 
TTTGATCCAG AATCCGGCGC TGCTCCCACA GTGAACAGCA GCTCCAGAAG CTCTTCGCCC 
TCTAAGGTGA TGGATGAGGG CAAGGTTAGT ATGGCTGCAA AAGGGCCCCC TCCCTTTCCA 
GGGACACCCC TCATGAGCTC CCCTGTGGGA GGCCCTCTAC TGCCACCCAT CCGATATGGA 
CCACCTCCTC AACTCTGTGG GCCTTTCGGA CCTCGGCCAC TCCCTCCACC CTTTGGTCCT 
GGTATGCGTC CACCGCTAGG CTTAAGAGAA TATGCACCAG GTGTTCCACC GGGAAAACGG 
GACCTGCCTC TCGACCCTCG AGAATTTTTA CCACCAGGAC ATGCACCTTT TAGACCTTTA 
GGCTCTCTTG GCCCAAGAGA GTACTTTTTT CCTGGTACCC GATTACCACC CCCCAATCAT 
GGGCCCCAGG ATTATCCACC TTCCTCTGCT GCAAGAGACT TACCGCCCTC CGGCTCCAGA 
GATGAGCCCC CGCCTGCCTC TCAGGGCGCT AGCCAGGACT GCTCACCGGC TTTAAAACAG 
AGCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831923.1
CDS	59..5707
Translation	MAAAQGLLFWLLLLGPPWRVPGQPEQDPGRRFSQLKLCADEECSMLMYRGEALEDFTGPDCRFVNFKKGDTVYVYYKLAGGSPEVWAGSVGHTFGYFPKDLIQVVHEYTQEELQVPTDETDFVCFDGGRDDFDNYNVEDLLGFLELYDSATEDSEKVKEKTAQRVEEPPEASNESDAEPEPGEPNSEESESVLSENTAELRERSEAQKSHPQVNSQTGHAQGERTSFESFGEMLQDKLKVPDSENNKTSNSSQVSHEQEKIDAYKLLKTEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFVEDLDPEYYTVGKEEEENKEDFDELPLLTFTDGEDTKSPGHSGIEKHPTEKEQNSNKEHKVEETQPPGIKKGDKEIPKHREDTVFSDVMEGEENTDTDLESSDSKEEDDPLVMDSRLGKPRPEDHTDPEKAADHLVNVEVPKADSDDDPEVGAGLHMKDKGRKVEEPRRDWVQHEVGLEDETQEDQAVQGSSQSGHLRSSPAAEKSTETLKSAFANQENDLKGAAVHISKEMLHEEKPSGRSLEGASKSDSVPQAKAAGNQGEEGKTEREPVGVAAPSGDHQPNASKDSVDEVDGSISGPKPHVLSGEHPSAELIKDRLLKLQNQTRFSSPDDMGLPGDLEKKRPILERKLSWQQGGVAAAVNKQVSEKRELPEEEVTRVTKDASDEGQEVRKTGQTDSIEGRGFRPKEPNPEDEDYSPEELLEDENAVSAKQSKERSPEIQDKRLDVDLQNPEKPVSGAIKTDPETEKNKEETRHVSENERKNETAGKAVDSLGRDAGGPVVEKEGSSPVHQKVQRPSEGSDVPGKKQNQTPELGEASQKKDPDYLKEDNHEGHPKTSGLMEKPGVEPSKEDDEHAEKFVDPGSRGSASEDPDDDPFPWAPHAPVQPEESVHLEDLPIISSFFKDQQSLQRFQKYFDIHKLEAMFQDMSSKLKSAQRESLPYNVEKVLDKVFRAWESHILTEAENMLDARVTENRDLETKDSSVFEEAAVLDDVQDLIYFVRYKHSTVEETAPPAAAQPVEGGWDGPAEDTQPPLEENFPQEHMEVPLMQIPKEPGHLAQPVTRDMGTSGVAQKPQTEEDGDPGIITPQGTPVDADDAQKQLAANTEEPASVTPLENAIAFIYSLVFHLTKTLLATLPDDVQPGPDFYGLPWKPVLITASLGIVSFAVFFWRTVLAVKSRVYQVTEQQISEKLKNIMKENAELVQKLSSYEQKIKESKKHVQETKKQNMILSDEAIKFKDKIKSLEETNEILGDTAKSLRAMLESEREQNAKNQDLISENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIRSFEKSQKDLEVALTHKDDNINALTNCITQLNRLDCESESEDQNKGGSESDELANGEVGGDRSEKVKNQIKQMMDVSRTQTAISVVEEDLKLLQCKLRASMSTKCNLEDQIKKLEEDRSSLQSAKTVLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKAVLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMMQEEPVIVKPMPGRPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTADPPARPLSATLNRREMPRSEFDPESGAAPTVNSSSRSSSPSKVMDEGKVSMAAKGPPPFPGTPLMSSPVGGPLLPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREYAPGVPPGKRDLPLDPREFLPPGHAPFRPLGSLGPREYFFPGTRLPPPNHGPQDYPPSSAARDLPPSGSRDEPPPASQGASQDCSPALKQSP

Target ORF information:

RefSeq Version	XM_024976155.1
Organism	Bos taurus(cattle)
Definition	Bos taurus MIA family member 3, ER export factor (MIA3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024976155.1

ORF Insert Sequence:

ATGGCTGCGG CGCAGGGGCT GCTCTTCTGG CTGCTCCTGC TCGGGCCGCC TTGGCGCGTG 
CCCGGCCAGC CGGAGCAGGA CCCCGGCCGG CGCTTCTCGC AGCTCAAACT CTGCGCGGAC 
GAAGAATGCA GCATGCTAAT GTACCGGGGT GAGGCTCTAG AAGATTTTAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGAGAC ACTGTATATG TCTACTATAA ACTGGCAGGG 
GGATCACCTG AAGTTTGGGC TGGAAGTGTT GGCCATACTT TTGGATATTT TCCAAAGGAT 
TTAATCCAAG TAGTCCATGA ATATACTCAG GAAGAGCTGC AAGTTCCAAC AGATGAGACA 
GATTTCGTTT GTTTTGATGG GGGAAGAGAT GATTTTGATA ATTATAATGT CGAAGACCTT 
TTAGGATTTT TGGAATTGTA CGATTCTGCC ACTGAAGATT CTGAAAAAGT TAAAGAAAAA 
ACTGCTCAGC GTGTGGAAGA ACCTCCTGAA GCATCTAATG AAAGTGACGC TGAACCTGAG 
CCCGGAGAAC CCAACTCAGA GGAAAGTGAA AGCGTACTCT CAGAAAATAC TGCGGAACTC 
AGGGAAAGAT CTGAGGCTCA GAAGAGCCAC CCCCAGGTAA ACAGTCAAAC AGGTCATGCT 
CAGGGAGAGC GAACTTCATT TGAATCTTTT GGAGAAATGC TGCAAGATAA GTTAAAAGTG 
CCAGACAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCGCACGA GCAGGAGAAG 
ATCGATGCTT ATAAACTTTT GAAAACAGAA ATGACTCTGG ACTTGAAAAC CAAATTTGGC 
TCAACTGCAG ATGCACTGGT ATCTGATGAT GAGACAACCA GGCTTGTTAC TTCATTAGAG 
GACGATTTTG TTGAGGATTT GGATCCTGAG TATTACACAG TGGGAAAGGA AGAAGAGGAG 
AATAAAGAAG ACTTCGATGA GCTGCCGTTA CTAACCTTTA CAGATGGAGA AGATACGAAA 
AGTCCAGGGC ACTCCGGAAT TGAGAAACAC CCAACAGAGA AAGAGCAGAA TTCAAACAAA 
GAGCATAAAG TTGAGGAAAC TCAGCCCCCA GGCATCAAAA AGGGTGATAA AGAGATACCG 
AAACACCGGG AAGATACTGT CTTCTCTGAT GTTATGGAAG GGGAAGAAAA CACAGACACA 
GATTTAGAGA GTTCTGATTC AAAGGAAGAG GATGACCCAT TAGTCATGGA TAGCAGATTG 
GGGAAACCAC GACCAGAAGA TCATACTGAC CCTGAAAAGG CAGCAGACCA CCTTGTGAAT 
GTAGAAGTCC CCAAAGCAGA CAGTGACGAC GACCCGGAAG TGGGCGCAGG ACTTCACATG 
AAAGACAAGG GGCGGAAAGT TGAGGAGCCC AGGAGGGACT GGGTGCAGCA TGAGGTAGGG 
CTAGAGGACG AGACGCAAGA AGACCAGGCT GTGCAGGGCT CTTCTCAGAG TGGCCACCTC 
AGGTCTTCAC CAGCTGCTGA AAAAAGTACA GAAACATTAA AATCAGCCTT TGCTAACCAA 
GAGAATGACC TGAAAGGAGC AGCCGTTCAC ATCTCCAAAG AAATGCTTCA CGAAGAAAAG 
CCCTCAGGGC GGAGTTTGGA AGGTGCCTCA AAGAGTGACT CAGTCCCGCA AGCAAAAGCG 
GCGGGGAATC AAGGGGAGGA AGGAAAGACT GAACGGGAAC CTGTGGGCGT GGCAGCCCCC 
TCGGGCGATC ATCAGCCTAA TGCTTCCAAA GACAGCGTGG ACGAAGTAGA CGGTTCAATA 
AGTGGGCCTA AACCACACGT GCTTTCGGGG GAGCATCCGA GCGCAGAACT TATAAAGGAT 
CGGCTTCTTA AACTTCAAAA TCAGACCAGG TTCTCCTCTC CAGATGACAT GGGCTTGCCA 
GGAGACCTGG AAAAAAAGCG GCCCATTCTG GAAAGAAAGC TTTCCTGGCA ACAAGGAGGT 
GTGGCTGCCG CAGTTAATAA GCAAGTGAGC GAAAAGAGAG AGCTCCCTGA AGAAGAGGTC 
ACCAGGGTCA CCAAGGATGC CTCAGATGAA GGGCAGGAAG TAAGAAAAAC AGGCCAAACT 
GATAGCATAG AAGGGCGAGG CTTCCGCCCA AAAGAACCAA ACCCAGAAGA TGAAGATTAC 
AGCCCTGAAG AACTCCTGGA GGATGAAAAT GCTGTAAGTG CAAAGCAGTC TAAAGAAAGA 
AGCCCTGAGA TTCAGGATAA GAGGTTAGAT GTTGACCTGC AAAACCCTGA AAAACCTGTT 
TCAGGTGCCA TTAAGACTGA TCCAGAAACT GAAAAAAACA AAGAAGAAAC TAGGCATGTA 
TCGGAAAATG AAAGAAAAAA TGAGACTGCG GGTAAAGCGG TAGACAGTCT GGGCAGGGAC 
GCAGGTGGCC CGGTGGTGGA AAAAGAAGGA AGCTCTCCTG TGCATCAGAA GGTACAGAGA 
CCCTCTGAAG GAAGTGACGT TCCTGGCAAG AAACAAAATC AGACTCCAGA GTTAGGGGAA 
GCGTCTCAGA AGAAAGATCC TGATTATCTT AAAGAAGACA ACCATGAGGG CCACCCAAAG 
ACCTCAGGGC TCATGGAGAA GCCTGGGGTA GAACCCTCGA AAGAGGACGA CGAGCATGCA 
GAGAAATTCG TGGACCCAGG AAGCCGGGGG TCTGCTTCCG AGGACCCTGA CGATGACCCG 
TTCCCCTGGG CTCCACATGC CCCTGTGCAG CCTGAGGAGA GTGTCCATTT GGAGGACTTG 
CCCATAATCA GCAGCTTCTT TAAAGATCAG CAGTCTCTGC AGCGATTCCA GAAGTACTTT 
GACATCCACA AGCTGGAAGC CATGTTCCAG GACATGTCAT CCAAGCTGAA GTCAGCTCAG 
CGTGAGAGCC TGCCCTATAA CGTGGAGAAA GTCCTCGATA AGGTCTTTCG TGCTTGGGAG 
TCACACATTC TGACAGAGGC AGAGAACATG CTTGATGCTC GCGTGACGGA AAACAGAGAC 
CTGGAAACGA AGGATAGTAG CGTCTTTGAG GAGGCTGCTG TGCTCGATGA TGTTCAAGAC 
CTGATCTATT TCGTCAGGTA CAAACACTCC ACAGTGGAGG AGACTGCTCC CCCAGCAGCG 
GCACAGCCTG TAGAGGGAGG CTGGGATGGA CCAGCAGAAG ACACACAGCC ACCACTGGAG 
GAGAACTTCC CACAGGAGCA CATGGAAGTT CCTCTGATGC AGATTCCCAA AGAGCCCGGC 
CATTTGGCTC AACCCGTGAC CAGGGACATG GGTACCTCAG GAGTGGCCCA GAAGCCGCAA 
ACCGAGGAAG ATGGAGACCC AGGGATAATT ACACCACAAG GCACTCCTGT CGATGCTGAT 
GATGCCCAAA AGCAGCTGGC AGCAAATACT GAAGAACCAG CAAGTGTCAC GCCTCTGGAA 
AATGCAATTG CTTTCATATA CTCACTTGTG TTTCATTTAA CTAAGACGCT CCTTGCTACA 
TTGCCTGATG ATGTCCAGCC TGGGCCTGAT TTTTATGGAC TGCCATGGAA GCCTGTACTT 
ATCACTGCCT CCTTAGGAAT TGTTTCATTT GCCGTTTTCT TCTGGAGAAC TGTCCTTGCT 
GTAAAGAGTA GAGTATATCA AGTCACTGAA CAGCAAATTT CTGAGAAGTT GAAGAATATC 
ATGAAAGAAA ACGCAGAACT TGTACAAAAA TTGTCAAGTT ATGAGCAGAA GATCAAGGAA 
TCAAAGAAAC ATGTTCAAGA AACCAAGAAA CAAAACATGA TTCTTTCTGA TGAAGCAATT 
AAATTTAAGG ATAAAATCAA GAGTCTTGAA GAAACTAATG AAATTCTGGG AGACACAGCT 
AAAAGTTTAC GCGCTATGTT AGAGTCTGAG AGAGAACAGA ATGCCAAGAA TCAGGACTTG 
ATATCAGAAA ATAAGAAATC TATAGAGAAG TTAAAGGATG TTATCTCAGT GAATGCCTCA 
GAATTTTCAG AGGTTCAAAT TGCCCTTAAT GAAGCTAAAC TTAGTGAAGA GAAAGTGAAG 
TCTGAATGCC ATCGGGTTCA AGAAGAAAAT GCTAGACTTA AGAAGAAGAA AGAGCAGTTG 
CAGCAAGAAA TCAAAGACTG GAGTAAATCA CATGCTGAGC TCAGTGAGCA AATCAGATCA 
TTTGAGAAGT CTCAGAAAGA TCTAGAAGTA GCTCTCACTC ACAAAGATGA TAATATTAAT 
GCTTTGACTA ATTGCATTAC ACAGTTGAAT CGGTTAGATT GTGAATCTGA ATCTGAGGAT 
CAAAATAAAG GAGGAAGTGA ATCAGATGAA TTAGCAAATG GAGAAGTGGG AGGTGACCGG 
AGTGAGAAGG TGAAAAATCA GATTAAGCAG ATGATGGACG TTTCTCGGAC ACAGACTGCA 
ATATCAGTAG TTGAAGAGGA TCTGAAACTT TTACAGTGTA AACTCAGAGC CTCCATGTCC 
ACTAAGTGTA ACCTGGAAGA CCAAATAAAG AAATTAGAAG AGGATCGCAG CTCACTTCAG 
TCTGCCAAAA CTGTGCTGGA AGATGAATGC AAAACACTGA GGCAGAAAGT AGAGATTCTA 
AATGAACTCT ATCAGCAGAA GGAGATGGCT CTACAAAAGA AATTGAGTCA AGAAGAGTAT 
GAGCGGCAAG AAAGAGAGCA GCGGCTGTCA GCTGCAGATG AAAAGGCGGT TTTGGCGGCA 
GAGGAAGTGA AAACTTACAA GCGGAGAATT GAAGAAATGG AGGATGAACT ACAGAAAACA 
GAGCGCTCAT TTAAAAACCA GATTGCTACT CATGAGAAGA AAGCTCACGA TAACTGGCTC 
AAAGCTCGTG CTGCAGAAAG AGCTATAGCT GAAGAGAAGA GAGAAGCTGC CAACTTGAGA 
CATAAATTAT TGGAACTAAC CCAAAAGATG GCAATGATGC AAGAAGAACC TGTGATTGTA 
AAACCAATGC CAGGAAGACC CAACACACAA AACCCTCCAC GGAGAGGTCC CCTGAGCCAG 
AATGGCTCTT TTGGCCCATC TCCTGTGAGC GGTGGAGAAT GCTCCCCACC GCTGACGGCA 
GACCCGCCCG CAAGACCTCT GTCTGCTACG CTTAATCGAA GAGAGATGCC TCGAAGTGAA 
TTTGATCCAG AATCCGGCGC TGCTCCCACA GTGAACAGCA GCTCCAGAAG CTCTTCGCCC 
TCTAAGGTGA TGGATGAGGG CAAGGTTAGT ATGGCTGCAA AAGGGCCCCC TCCCTTTCCA 
GGGACACCCC TCATGAGCTC CCCTGTGGGA GGCCCTCTAC TGCCACCCAT CCGATATGGA 
CCACCTCCTC AACTCTGTGG GCCTTTCGGA CCTCGGCCAC TCCCTCCACC CTTTGGTCCT 
GGTATGCGTC CACCGCTAGG CTTAAGAGAA TATGCACCAG GTGTTCCACC GGGAAAACGG 
GACCTGCCTC TCGACCCTCG AGAATTTTTA CCACCAGGAC ATGCACCTTT TAGACCTTTA 
GGCTCTCTTG GCCCAAGAGA GTACTTTTTT CCTGGTACCC GATTACCACC CCCCAATCAT 
GGGCCCCAGG ATTATCCACC TTCCTCTGCT GCAAGAGACT TACCGCCCTC CGGCTCCAGA 
GATGAGCCCC CGCCTGCCTC TCAGGGCGCT AGCCAGGACT GCTCACCGGC TTTAAAACAG 
AGCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( NM_001166571.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001160043.1 Bos taurus melanoma inhibitory activity family, member 3 (MIA3), mRNA.

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CloneID	OBa44547
Clone ID Related Accession (Same CDS sequence)	NM_001166571.1
Accession Version	NM_001166571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(cattle)
Product	melanoma inhibitory activity protein 3 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03070852.1, AAFC02070526.1 and AAFC03070853.1. On Oct 28, 2009 this sequence version replaced XM_586030.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00000728, SAMN01093727 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCGG CGCAGGGGCT GCTCTTCTGG CTGCTCCTGC TCGGGCCGCC TTGTCGCGTG 
CCCGGCCAGC CGGAGCAGGA CCCCGGCCGG CGGTTCTCGC AGTTCAAACT TTGCGCGGAC 
GAAGAATGCT GCATGCTAAT GTACCGGGGT GAGGCTCTAG AAGATTTTAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGAGAC ACTGTATATG TCTACTATAA ACTGGCAGGG 
GGATCACCTG AAGTTTGGGC TGGAAGTGTT GGCCATACTT TTGGATATTT TCCAAAGGAT 
TTAATCCAAG TAGTCCATGA ATATACTCAG GAAGAGCTGC AAGTTCCAAC AGATGAGACA 
GATTTCGTTT GTTTTGATGG GGGAAGAGAT GATTTTGATA ATTATAATGT CGAAGACCTT 
TTAGGATTTT TGGAATTGTA CGATTCTGCC ACTGAAGATT CTGAAAAAGT TAAAGAAAAA 
ACTGCTCAGC GTGTGGAAGA ACCTCCTGAA GCATCTAATG AAAGTGACGC TGAACCTGAG 
CCCGGAGAAC CCAACTCAGA GGAAAGTGAA AGCGTACTCT CAGAAAATAC TGCGGAACTC 
AGGGAAAGAT CTGAGGCTCA GAAGAGCCAC CCCCAGGTAA ACAGTCAAAC AGGTCATGCT 
CAGGGAGAGC GAACTTCATT TGAATCTTTT GGAGAAATGC TGCAAGATAA GTTAAAAGTG 
CCAGACAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCGCACGA GCAGGAGAAG 
ATCGATGCTT ATAAACTTTT GAAAACAGAA ATGACTCTGG ACTTGAAAAC CAAATTTGGC 
TCAACTGCAG ATGCACTGGT ATCTGATGAT GAGACAACCA GGCTTGTTAC TTCATTAGAG 
GACGATTTTG TTGAGGATTT GGATCCTGAG TATTACACAG TGGGAAAGGA AGAAGAGGAG 
AATAAAGAAG ACTTCGATGA GCTGCCGTTA CTAACCTTTA CAGATGGAGA AGATACGAAA 
AGTCCAGGGC ACTCCGGAAT TGAGAAACAC CCAACAGAGA AAGAGCAGAA TTCAAACAAA 
GAGCATAAAG TTGAGGAAAC TCAGCCCCCA GGCATCAAAA AGGGTGATAA AGAGATACCG 
AAACACCGGG AAGATACTGT CTTCTCTGAT GTTATGGAAG GGGAAGAAAA CACAGACACA 
GATTTAGAGA GTTCTGATTC AAAGGAAGAG GATGACCCAT TAGTCATGGA TAGCAGATTG 
GGGAAACCAC GACCAGAAGA TCATACTGAC CCTGAAAAGG CAGCAGACCA CCTTGTGAAT 
GTAGAAGTCC CCAAAGCAGA CAGTGACGAC GACCCGGAAG TGGGCGCAGG ACTTCACATG 
AAAGACAAGG GGCGGAAAGT TGAGGAGCCC AGGAGGGACT GGGTGCAGCA TGAGGTAGGG 
CTAGAGGACG AGACGCAAGA AGACCAGGCT GTGCAGGGCT CTTCTCAGAG TGGCCACCTC 
AGGTCTTCAC CAGCTGCTGA AAAAAGTACA GAAACATTAA AATCAGCCTT TGCTAACCAA 
GAGAATGACC TGAAAGGAGC AGCCGTTCAC ATCTCCAAAG AAATGCTTCA CGAAGAAAAG 
CCCTCAGGGC GGAGTTTGGA AGGTGCCTCA AAGAGTGACT CAGTCCCGCA AGCAAAAGCG 
GCGGGGAATC AAGGGGAGGA AGGAAAGACT GAACGGGAAC CTGTGGGCGT GGCAGCCCCC 
TCGGGCGATC ATCAGCCTAA TGCTTCCAAA GACAGCGTGG ACGAAGTAGA CGGTTCAATA 
AGTGGGCCTA AACCACACGT GCTTTCGGGG GAGCATCCGA GCGCAGAACT TATAAAGGAT 
CGGCTTCTTA AACTTCAAAA TCAGACCAGG TTCTCCTCTC CAGATGACAT GGGCTTGCCA 
GGAGACCTGG AAAAAAAGCG GCCCATTCTG GAAAGAAAGC TTTCCTGGCA ACAAGGAGGT 
GTGGCTGCCG CAGTTAATAA GCAAGTGAGC GAAAAGAGAG AGCTCCCTGA AGAAGAGGTC 
ACCAGGGTCA CCAAGGATGC CTCAGATGAA GGGCAGGAAG TAAGAAAAAC AGGCCAAACT 
GATAGCATAG AAGGGCGAGG CTTCCGCCCA AAAGAACCAA ACCCAGAAGA TGAAGATTAC 
AGCCCTGAAG AACTCCTGGA GGATGAAAAT GCTGTAAGTG CAAAGCAGTC TAAAGAAAGA 
AGCCCTGAGA TTCAGGATAA GAGGTTAGAT GTTGACCTGC AAAACCCTGA AAAACCTGTT 
TCAGGTGCCA TTAAGACTGA TCCAGAAACT GAAAAAAACA AAGAAGAAAC TAGGCATGTA 
TCGGAAAATG AAAGAAAAAA TGAGACTGCG GGTAAAGCGG TAGACAGTCT GGGCAGGGAC 
GCAGGTGGCC CGGTGGTGGA AAAAGAAGGA AGCTCTCCTG TGCATCAGAA GGTACAGAGA 
CCCTCTGAAG GAAGTGACGT TCCTGGCAAG AAACAAAATC AGACTCCAGA GTTAGGGGAA 
GCGTCTCAGA AGAAAGATCC TGATTATCTT AAAGAAGACA ACCATGAGGG CCACCCAAAG 
ACCTCAGGGC TCATGGAGAA GCCTGGGGTA GAACCCTCGA AAGAGGACGA CGAGCATGCA 
GAGAAATTCG TGGACCCAGG AAGCCGGGGG TCTGCTTCCG AGGACCCTGA CGATGACCCG 
TTCCCCTGGG CTCCACATGC CCCTGTGCAG CCTGAGGAGA GTGTCCATTT GGAGGACTTG 
CCCATAATCA GCAGCTTCTT TAAAGATCAG CAGTCTCTGC AGCGATTCCA GAAGTACTTT 
GACATCCACA AGCTGGAAGC CATGTTCCAG GACATGTCAT CCAAGCTGAA GTCAGCTCAG 
CGTGAGAGCC TGCCCTATAA CGTGGAGAAA GTCCTCGATA AGGTCTTTCG TGCTTGGGAG 
TCACACATTC TGACAGAGGC AGAGAACATG CTTGATGCTC GCGTGACGGA AAACAGAGAC 
CTGGAAACGA AGGATAGTAG CGTCTTTGAG GAGGCTGCTG TGCTCGATGA TGTTCAAGAC 
CTGATCTATT TCGTCAGGTA CAAACACTCC ACAGTGGAGG AGACTGCTCC CCCAGCAGCG 
GCACAGCCTG TAGAGGGAGG CTGGGATGGA CCAGCAGAAG ACACACAGCC ACCACTGGAG 
GAGAACTTCC CACAGGAGCA CATGGAAGTT CCTCTGATGC AGATTCCCAA AGAGCCCGGC 
CATTTGGCTC AACCCGTGAC CAGGGACATG GGTACCTCAG GAGTGGCCCA GAAGCCGCAA 
ACCGAGGAAG ATGGAGACCC AGGGATAATT ACACCACAAG GCACTCCTGT CGATGCTGAT 
GATGCCCAAA AGCAGCTGGC AGCAAATACT GAAGAACCAG CAAGTGTCAC GCCTCTGGAA 
AATGCAATTG CTTTCATATA CTCACTTGTG TTTCATTTAA CTAAGACGCT CCTTGCTACA 
TTGCCTGATG ATGTCCAGCC TGGGCCTGAT TTTTATGGAC TGCCATGGAA GCCTGTACTT 
ATCACTGCCT CCTTAGGAAT TGTTTCATTT GCCGTTTTCT TCTGGAGAAC TGTCCTTGCT 
GTAAAGAGTA GAGTATATCA AGTCACTGAA CAGCAAATTT CTGAGAAGTT GAAGAATATC 
ATGAAAGAAA ACGCAGAACT TGTACAAAAA TTGTCAAGTT ATGAGCAGAA GATCAAGGAA 
TCAAAGAAAC ATGTTCAAGA AACCAAGAAA CAAAACATGA TTCTTTCTGA TGAAGCAATT 
AAATTTAAGG ATAAAATCAA GAGTCTTGAA GAAACTAATG AAATTCTGGG AGACACAGCT 
AAAAGTTTAC GCGCTATGTT AGAGTCTGAG AGAGAACAGA ATGCCAAGAA TCAGGACTTG 
ATATCAGAAA ATAAGAAATC TATAGAGAAG TTAAAGGATG TTATCTCAGT GAATGCCTCA 
GAATTTTCAG AGGTTCAAAT TGCCCTTAAT GAAGCTAAAC TTAGTGAAGA GAAAGTGAAG 
TCTGAATGCC ATCGGGTTCA AGAAGAAAAT GCTAGACTTA AGAAGAAGAA AGAGCAGTTG 
CAGCAAGAAA TCAAAGACTG GAGTAAATCA CATGCTGAGC TCAGTGAGCA AATCAGATCA 
TTTGAGAAGT CTCAGAAAGA TCTAGAAGTA GCTCTCACTC ACAAAGATGA TAATATTAAT 
GCTTTGACTA ATTGCATTAC ACAGTTGAAT CGGTTAGATT GTGAATCTGA ATCTGAGGAT 
CAAAATAAAG GAGGAAGTGA ATCAGATGAA TTAGCAAATG GAGAAGTGGG AGGTGACCGG 
AGTGAGAAGG TGAAAAATCA GATTAAGCAG ATGATGGACG TTTCTCGGAC ACAGACTGCA 
ATATCAGTAG TTGAAGAGGA TCTGAAACTT TTACAGTGTA AACTCAGAGC CTCCATGTCC 
ACTAAGTGTA ACCTGGAAGA CCAAATAAAG AAATTAGAAG AGGATCGCAG CTCACTTCAG 
TCTGCCAAAA CTGTGCTGGA AGATGAATGC AAAACACTGA GGCAGAAAGT AGAGATTCTA 
AATGAACTCT ATCAGCAGAA GGAGATGGCT CTACAAAAGA AATTGAGTCA AGAAGAGTAT 
GAGCGGCAAG AAAGAGAGCA GCGGCTGTCA GCTGCAGATG AAAAGGCGGT TTTGGCGGCA 
GAGGAAGTGA AAACTTACAA GCGGAGAATT GAAGAAATGG AGGATGAACT ACAGAAAACA 
GAGCGCTCAT TTAAAAACCA GATTGCTACT CATGAGAAGA AAGCTCACGA TAACTGGCTC 
AAAGCTCGTG CTGCAGAAAG AGCTATAGCT GAAGAGAAGA GAGAAGCTGC CAACTTGAGA 
CATAAATTAT TGGAACTAAC CCAAAAGATG GCAATGATGC AAGAAGAACC TGTGATTGTA 
AAACCAATGC CAGGAAGACC CAACACACAA AACCCTCCAC GGAGAGGTCC CCTGAGCCAG 
AATGGCTCTT TTGGCCCATC TCCTGTGAGC GGTGGAGAAT GCTCCCCACC GCTGACGGCA 
GACCCGCCCG CAAGACCTCT GTCTGCTACG CTTAATCGAA GAGAGATGCC TCGAAGTGAA 
TTTGGATCAG TGGATGGGCC TCTCCCTCGA CCTCGATGGG CCTCTGAGGC ATCTGGGAAA 
CCCTCTGCCT CTGATCCAGA ATCCGGCGCT GCTCCCACAG TGAACAGCAG CTCCAGAAGC 
TCTTCGCCCT CTAAGGTGAT GGATGAGGGC AAGGTTAGTA TGGCTGCAAA AGGGCCCCCT 
CCCTTTCCAG GGACACCCCT CATGAGCTCC CCTGTGGGAG GCCCTCTACT GCCACCCATC 
CGATATGGAC CACCTCCTCA ACTCTGTGGG CCTTTCGGAC CTCGGCCACT CCCTCCACCC 
TTTGGTCCTG GTATGCGTCC ACCGCTAGGC TTAAGAGAAT ATGCACCAGG TGTTCCACCG 
GGAAAACGGG ACCTGCCTCT CGACCCTCGA GAATTTTTAC CACCAGGACA TGCACCTTTT 
AGACCTTTAG GCTCTCTTGG CCCAAGAGAG TACTTTTTTC CTGGTACCCG ATTACCACCC 
CCCAATCATG GGCCCCAGGA TTATCCACCT TCCTCTGCTG CAAGAGACTT ACCGCCCTCC 
GGCTCCAGAG ATGAGCCCCC GCCTGCCTCT CAGGGCGCTA GCCAGGACTG CTCACCGGCT 
TTAAAACAGA GCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001160043.1
CDS	1..5718
Translation	MAAAQGLLFWLLLLGPPCRVPGQPEQDPGRRFSQFKLCADEECCMLMYRGEALEDFTGPDCRFVNFKKGDTVYVYYKLAGGSPEVWAGSVGHTFGYFPKDLIQVVHEYTQEELQVPTDETDFVCFDGGRDDFDNYNVEDLLGFLELYDSATEDSEKVKEKTAQRVEEPPEASNESDAEPEPGEPNSEESESVLSENTAELRERSEAQKSHPQVNSQTGHAQGERTSFESFGEMLQDKLKVPDSENNKTSNSSQVSHEQEKIDAYKLLKTEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFVEDLDPEYYTVGKEEEENKEDFDELPLLTFTDGEDTKSPGHSGIEKHPTEKEQNSNKEHKVEETQPPGIKKGDKEIPKHREDTVFSDVMEGEENTDTDLESSDSKEEDDPLVMDSRLGKPRPEDHTDPEKAADHLVNVEVPKADSDDDPEVGAGLHMKDKGRKVEEPRRDWVQHEVGLEDETQEDQAVQGSSQSGHLRSSPAAEKSTETLKSAFANQENDLKGAAVHISKEMLHEEKPSGRSLEGASKSDSVPQAKAAGNQGEEGKTEREPVGVAAPSGDHQPNASKDSVDEVDGSISGPKPHVLSGEHPSAELIKDRLLKLQNQTRFSSPDDMGLPGDLEKKRPILERKLSWQQGGVAAAVNKQVSEKRELPEEEVTRVTKDASDEGQEVRKTGQTDSIEGRGFRPKEPNPEDEDYSPEELLEDENAVSAKQSKERSPEIQDKRLDVDLQNPEKPVSGAIKTDPETEKNKEETRHVSENERKNETAGKAVDSLGRDAGGPVVEKEGSSPVHQKVQRPSEGSDVPGKKQNQTPELGEASQKKDPDYLKEDNHEGHPKTSGLMEKPGVEPSKEDDEHAEKFVDPGSRGSASEDPDDDPFPWAPHAPVQPEESVHLEDLPIISSFFKDQQSLQRFQKYFDIHKLEAMFQDMSSKLKSAQRESLPYNVEKVLDKVFRAWESHILTEAENMLDARVTENRDLETKDSSVFEEAAVLDDVQDLIYFVRYKHSTVEETAPPAAAQPVEGGWDGPAEDTQPPLEENFPQEHMEVPLMQIPKEPGHLAQPVTRDMGTSGVAQKPQTEEDGDPGIITPQGTPVDADDAQKQLAANTEEPASVTPLENAIAFIYSLVFHLTKTLLATLPDDVQPGPDFYGLPWKPVLITASLGIVSFAVFFWRTVLAVKSRVYQVTEQQISEKLKNIMKENAELVQKLSSYEQKIKESKKHVQETKKQNMILSDEAIKFKDKIKSLEETNEILGDTAKSLRAMLESEREQNAKNQDLISENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIRSFEKSQKDLEVALTHKDDNINALTNCITQLNRLDCESESEDQNKGGSESDELANGEVGGDRSEKVKNQIKQMMDVSRTQTAISVVEEDLKLLQCKLRASMSTKCNLEDQIKKLEEDRSSLQSAKTVLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKAVLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMMQEEPVIVKPMPGRPNTQNPPRRGPLSQNGSFGPSPVSGGECSPPLTADPPARPLSATLNRREMPRSEFGSVDGPLPRPRWASEASGKPSASDPESGAAPTVNSSSRSSSPSKVMDEGKVSMAAKGPPPFPGTPLMSSPVGGPLLPPIRYGPPPQLCGPFGPRPLPPPFGPGMRPPLGLREYAPGVPPGKRDLPLDPREFLPPGHAPFRPLGSLGPREYFFPGTRLPPPNHGPQDYPPSSAARDLPPSGSRDEPPPASQGASQDCSPALKQSP

Target ORF information:

RefSeq Version	NM_001166571.1
Organism	Bos taurus(cattle)
Definition	Bos taurus melanoma inhibitory activity family, member 3 (MIA3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166571.1

ORF Insert Sequence:

ATGGCTGCGG CGCAGGGGCT GCTCTTCTGG CTGCTCCTGC TCGGGCCGCC TTGTCGCGTG 
CCCGGCCAGC CGGAGCAGGA CCCCGGCCGG CGGTTCTCGC AGTTCAAACT TTGCGCGGAC 
GAAGAATGCT GCATGCTAAT GTACCGGGGT GAGGCTCTAG AAGATTTTAC AGGCCCGGAT 
TGTCGTTTTG TGAATTTTAA AAAAGGAGAC ACTGTATATG TCTACTATAA ACTGGCAGGG 
GGATCACCTG AAGTTTGGGC TGGAAGTGTT GGCCATACTT TTGGATATTT TCCAAAGGAT 
TTAATCCAAG TAGTCCATGA ATATACTCAG GAAGAGCTGC AAGTTCCAAC AGATGAGACA 
GATTTCGTTT GTTTTGATGG GGGAAGAGAT GATTTTGATA ATTATAATGT CGAAGACCTT 
TTAGGATTTT TGGAATTGTA CGATTCTGCC ACTGAAGATT CTGAAAAAGT TAAAGAAAAA 
ACTGCTCAGC GTGTGGAAGA ACCTCCTGAA GCATCTAATG AAAGTGACGC TGAACCTGAG 
CCCGGAGAAC CCAACTCAGA GGAAAGTGAA AGCGTACTCT CAGAAAATAC TGCGGAACTC 
AGGGAAAGAT CTGAGGCTCA GAAGAGCCAC CCCCAGGTAA ACAGTCAAAC AGGTCATGCT 
CAGGGAGAGC GAACTTCATT TGAATCTTTT GGAGAAATGC TGCAAGATAA GTTAAAAGTG 
CCAGACAGTG AAAACAACAA AACCAGCAAT AGTTCTCAGG TCTCGCACGA GCAGGAGAAG 
ATCGATGCTT ATAAACTTTT GAAAACAGAA ATGACTCTGG ACTTGAAAAC CAAATTTGGC 
TCAACTGCAG ATGCACTGGT ATCTGATGAT GAGACAACCA GGCTTGTTAC TTCATTAGAG 
GACGATTTTG TTGAGGATTT GGATCCTGAG TATTACACAG TGGGAAAGGA AGAAGAGGAG 
AATAAAGAAG ACTTCGATGA GCTGCCGTTA CTAACCTTTA CAGATGGAGA AGATACGAAA 
AGTCCAGGGC ACTCCGGAAT TGAGAAACAC CCAACAGAGA AAGAGCAGAA TTCAAACAAA 
GAGCATAAAG TTGAGGAAAC TCAGCCCCCA GGCATCAAAA AGGGTGATAA AGAGATACCG 
AAACACCGGG AAGATACTGT CTTCTCTGAT GTTATGGAAG GGGAAGAAAA CACAGACACA 
GATTTAGAGA GTTCTGATTC AAAGGAAGAG GATGACCCAT TAGTCATGGA TAGCAGATTG 
GGGAAACCAC GACCAGAAGA TCATACTGAC CCTGAAAAGG CAGCAGACCA CCTTGTGAAT 
GTAGAAGTCC CCAAAGCAGA CAGTGACGAC GACCCGGAAG TGGGCGCAGG ACTTCACATG 
AAAGACAAGG GGCGGAAAGT TGAGGAGCCC AGGAGGGACT GGGTGCAGCA TGAGGTAGGG 
CTAGAGGACG AGACGCAAGA AGACCAGGCT GTGCAGGGCT CTTCTCAGAG TGGCCACCTC 
AGGTCTTCAC CAGCTGCTGA AAAAAGTACA GAAACATTAA AATCAGCCTT TGCTAACCAA 
GAGAATGACC TGAAAGGAGC AGCCGTTCAC ATCTCCAAAG AAATGCTTCA CGAAGAAAAG 
CCCTCAGGGC GGAGTTTGGA AGGTGCCTCA AAGAGTGACT CAGTCCCGCA AGCAAAAGCG 
GCGGGGAATC AAGGGGAGGA AGGAAAGACT GAACGGGAAC CTGTGGGCGT GGCAGCCCCC 
TCGGGCGATC ATCAGCCTAA TGCTTCCAAA GACAGCGTGG ACGAAGTAGA CGGTTCAATA 
AGTGGGCCTA AACCACACGT GCTTTCGGGG GAGCATCCGA GCGCAGAACT TATAAAGGAT 
CGGCTTCTTA AACTTCAAAA TCAGACCAGG TTCTCCTCTC CAGATGACAT GGGCTTGCCA 
GGAGACCTGG AAAAAAAGCG GCCCATTCTG GAAAGAAAGC TTTCCTGGCA ACAAGGAGGT 
GTGGCTGCCG CAGTTAATAA GCAAGTGAGC GAAAAGAGAG AGCTCCCTGA AGAAGAGGTC 
ACCAGGGTCA CCAAGGATGC CTCAGATGAA GGGCAGGAAG TAAGAAAAAC AGGCCAAACT 
GATAGCATAG AAGGGCGAGG CTTCCGCCCA AAAGAACCAA ACCCAGAAGA TGAAGATTAC 
AGCCCTGAAG AACTCCTGGA GGATGAAAAT GCTGTAAGTG CAAAGCAGTC TAAAGAAAGA 
AGCCCTGAGA TTCAGGATAA GAGGTTAGAT GTTGACCTGC AAAACCCTGA AAAACCTGTT 
TCAGGTGCCA TTAAGACTGA TCCAGAAACT GAAAAAAACA AAGAAGAAAC TAGGCATGTA 
TCGGAAAATG AAAGAAAAAA TGAGACTGCG GGTAAAGCGG TAGACAGTCT GGGCAGGGAC 
GCAGGTGGCC CGGTGGTGGA AAAAGAAGGA AGCTCTCCTG TGCATCAGAA GGTACAGAGA 
CCCTCTGAAG GAAGTGACGT TCCTGGCAAG AAACAAAATC AGACTCCAGA GTTAGGGGAA 
GCGTCTCAGA AGAAAGATCC TGATTATCTT AAAGAAGACA ACCATGAGGG CCACCCAAAG 
ACCTCAGGGC TCATGGAGAA GCCTGGGGTA GAACCCTCGA AAGAGGACGA CGAGCATGCA 
GAGAAATTCG TGGACCCAGG AAGCCGGGGG TCTGCTTCCG AGGACCCTGA CGATGACCCG 
TTCCCCTGGG CTCCACATGC CCCTGTGCAG CCTGAGGAGA GTGTCCATTT GGAGGACTTG 
CCCATAATCA GCAGCTTCTT TAAAGATCAG CAGTCTCTGC AGCGATTCCA GAAGTACTTT 
GACATCCACA AGCTGGAAGC CATGTTCCAG GACATGTCAT CCAAGCTGAA GTCAGCTCAG 
CGTGAGAGCC TGCCCTATAA CGTGGAGAAA GTCCTCGATA AGGTCTTTCG TGCTTGGGAG 
TCACACATTC TGACAGAGGC AGAGAACATG CTTGATGCTC GCGTGACGGA AAACAGAGAC 
CTGGAAACGA AGGATAGTAG CGTCTTTGAG GAGGCTGCTG TGCTCGATGA TGTTCAAGAC 
CTGATCTATT TCGTCAGGTA CAAACACTCC ACAGTGGAGG AGACTGCTCC CCCAGCAGCG 
GCACAGCCTG TAGAGGGAGG CTGGGATGGA CCAGCAGAAG ACACACAGCC ACCACTGGAG 
GAGAACTTCC CACAGGAGCA CATGGAAGTT CCTCTGATGC AGATTCCCAA AGAGCCCGGC 
CATTTGGCTC AACCCGTGAC CAGGGACATG GGTACCTCAG GAGTGGCCCA GAAGCCGCAA 
ACCGAGGAAG ATGGAGACCC AGGGATAATT ACACCACAAG GCACTCCTGT CGATGCTGAT 
GATGCCCAAA AGCAGCTGGC AGCAAATACT GAAGAACCAG CAAGTGTCAC GCCTCTGGAA 
AATGCAATTG CTTTCATATA CTCACTTGTG TTTCATTTAA CTAAGACGCT CCTTGCTACA 
TTGCCTGATG ATGTCCAGCC TGGGCCTGAT TTTTATGGAC TGCCATGGAA GCCTGTACTT 
ATCACTGCCT CCTTAGGAAT TGTTTCATTT GCCGTTTTCT TCTGGAGAAC TGTCCTTGCT 
GTAAAGAGTA GAGTATATCA AGTCACTGAA CAGCAAATTT CTGAGAAGTT GAAGAATATC 
ATGAAAGAAA ACGCAGAACT TGTACAAAAA TTGTCAAGTT ATGAGCAGAA GATCAAGGAA 
TCAAAGAAAC ATGTTCAAGA AACCAAGAAA CAAAACATGA TTCTTTCTGA TGAAGCAATT 
AAATTTAAGG ATAAAATCAA GAGTCTTGAA GAAACTAATG AAATTCTGGG AGACACAGCT 
AAAAGTTTAC GCGCTATGTT AGAGTCTGAG AGAGAACAGA ATGCCAAGAA TCAGGACTTG 
ATATCAGAAA ATAAGAAATC TATAGAGAAG TTAAAGGATG TTATCTCAGT GAATGCCTCA 
GAATTTTCAG AGGTTCAAAT TGCCCTTAAT GAAGCTAAAC TTAGTGAAGA GAAAGTGAAG 
TCTGAATGCC ATCGGGTTCA AGAAGAAAAT GCTAGACTTA AGAAGAAGAA AGAGCAGTTG 
CAGCAAGAAA TCAAAGACTG GAGTAAATCA CATGCTGAGC TCAGTGAGCA AATCAGATCA 
TTTGAGAAGT CTCAGAAAGA TCTAGAAGTA GCTCTCACTC ACAAAGATGA TAATATTAAT 
GCTTTGACTA ATTGCATTAC ACAGTTGAAT CGGTTAGATT GTGAATCTGA ATCTGAGGAT 
CAAAATAAAG GAGGAAGTGA ATCAGATGAA TTAGCAAATG GAGAAGTGGG AGGTGACCGG 
AGTGAGAAGG TGAAAAATCA GATTAAGCAG ATGATGGACG TTTCTCGGAC ACAGACTGCA 
ATATCAGTAG TTGAAGAGGA TCTGAAACTT TTACAGTGTA AACTCAGAGC CTCCATGTCC 
ACTAAGTGTA ACCTGGAAGA CCAAATAAAG AAATTAGAAG AGGATCGCAG CTCACTTCAG 
TCTGCCAAAA CTGTGCTGGA AGATGAATGC AAAACACTGA GGCAGAAAGT AGAGATTCTA 
AATGAACTCT ATCAGCAGAA GGAGATGGCT CTACAAAAGA AATTGAGTCA AGAAGAGTAT 
GAGCGGCAAG AAAGAGAGCA GCGGCTGTCA GCTGCAGATG AAAAGGCGGT TTTGGCGGCA 
GAGGAAGTGA AAACTTACAA GCGGAGAATT GAAGAAATGG AGGATGAACT ACAGAAAACA 
GAGCGCTCAT TTAAAAACCA GATTGCTACT CATGAGAAGA AAGCTCACGA TAACTGGCTC 
AAAGCTCGTG CTGCAGAAAG AGCTATAGCT GAAGAGAAGA GAGAAGCTGC CAACTTGAGA 
CATAAATTAT TGGAACTAAC CCAAAAGATG GCAATGATGC AAGAAGAACC TGTGATTGTA 
AAACCAATGC CAGGAAGACC CAACACACAA AACCCTCCAC GGAGAGGTCC CCTGAGCCAG 
AATGGCTCTT TTGGCCCATC TCCTGTGAGC GGTGGAGAAT GCTCCCCACC GCTGACGGCA 
GACCCGCCCG CAAGACCTCT GTCTGCTACG CTTAATCGAA GAGAGATGCC TCGAAGTGAA 
TTTGGATCAG TGGATGGGCC TCTCCCTCGA CCTCGATGGG CCTCTGAGGC ATCTGGGAAA 
CCCTCTGCCT CTGATCCAGA ATCCGGCGCT GCTCCCACAG TGAACAGCAG CTCCAGAAGC 
TCTTCGCCCT CTAAGGTGAT GGATGAGGGC AAGGTTAGTA TGGCTGCAAA AGGGCCCCCT 
CCCTTTCCAG GGACACCCCT CATGAGCTCC CCTGTGGGAG GCCCTCTACT GCCACCCATC 
CGATATGGAC CACCTCCTCA ACTCTGTGGG CCTTTCGGAC CTCGGCCACT CCCTCCACCC 
TTTGGTCCTG GTATGCGTCC ACCGCTAGGC TTAAGAGAAT ATGCACCAGG TGTTCCACCG 
GGAAAACGGG ACCTGCCTCT CGACCCTCGA GAATTTTTAC CACCAGGACA TGCACCTTTT 
AGACCTTTAG GCTCTCTTGG CCCAAGAGAG TACTTTTTTC CTGGTACCCG ATTACCACCC 
CCCAATCATG GGCCCCAGGA TTATCCACCT TCCTCTGCTG CAAGAGACTT ACCGCCCTCC 
GGCTCCAGAG ATGAGCCCCC GCCTGCCTCT CAGGGCGCTA GCCAGGACTG CTCACCGGCT 
TTAAAACAGA GCCCATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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MIA3 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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