MIA3 gene cDNA ORF clone, Canis lupus familiaris(dog)

mRNA	Protein	Name
XM_022415455.1	XP_022271163.1	transport and Golgi organization protein 1 homolog isoform X4
XM_005640837.2	XP_005640894.1	melanoma inhibitory activity protein 3 isoform X3
XM_022415456.1	XP_022271164.1	transport and Golgi organization protein 1 homolog isoform X5
XM_014111164.2	XP_013966639.1	transport and Golgi organization protein 1 homolog isoform X6
XM_005640835.2	XP_005640892.1	melanoma inhibitory activity protein 3 isoform X1
XM_014111164.1	XP_013966639.1	melanoma inhibitory activity protein 3 isoform X5
XM_005640837.3	XP_005640894.1	transport and Golgi organization protein 1 homolog isoform X3
XM_014111163.1	XP_013966638.1	melanoma inhibitory activity protein 3 isoform X4
XM_005640836.2	XP_005640893.1	melanoma inhibitory activity protein 3 isoform X2
XM_005640835.3	XP_005640892.1	transport and Golgi organization protein 1 homolog isoform X1
XM_005640836.3	XP_005640893.1	transport and Golgi organization protein 1 homolog isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe285057	XM_022415456.1 Latest version!	Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe45302	XM_014111164.2 Latest version!	Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe285056	XM_022415455.1 Latest version!	Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe31714	XM_005640835.3 Latest version!	Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe31715	XM_005640836.3 Latest version!	Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe31716	XM_005640837.3 Latest version!	Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe45301	XM_014111163.1 Latest version!	Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
OCe45302	XM_014111164.1	Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCe31716	XM_005640837.2	Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe31714	XM_005640835.2	Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OCe31715	XM_005640836.2	Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

MIA3 ( XM_022415456.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022271164.1 Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe285057
Clone ID Related Accession (Same CDS sequence)	XM_022415456.1
Accession Version	XM_022415456.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2430bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transport and Golgi organization protein 1 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCG TTCCCGCCAC TGTGCCTTCT GTCACCGCCT CCCCGGGGGA CTCGGAGCTC 
CTGGGACCCC TGTCGGTGCT CTACGCGGCC CTCATAGCCA AGCTGCTGGA GTTAGTTGCT 
ACATTGCCTG ATGACGTTCA GCCTGGGCCT GATTTTTATG GCTTGCCATG GAAGCCTGTA 
CTTCTCACTG CCTTCTTGGG AATTGGTTCA TTTGCGGTCT TCTTCTGGAA AACTGTCCTT 
GTTGTAAAGA GTAGAGTCTA TCAAGTCACT GAACAACAAA TTTCTGAGAA GTTGAAGAAC 
ATCATGAAAG AAAATGCAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATTAAG 
GAATCAAAAA AACATGTTCA AGAAACCAAG AAACAAAATA TAATTCTGTC TGATGAAGCA 
ATTAAATTTA AGGATAAAAT CAAGAAACTT GAAGAAACAA ATGAAATTCT GGACGATACA 
GCTAAAAATC TGCGTGTTAT GTTAGAATCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC 
TTGATACTGG AAAACAAGAA ATCTATAGAG AAGCTGAAGG ATGTTATTTC AGTGAATGCC 
TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAGGTG 
AAGTCTGAAT GCCATCGAGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA GAAGGAACAG 
TTGCAGCAAG AAATCAAAGA TTGGAGCAAA TCACACGCTG AGCTCAGTGA GCAAATCAAA 
TCATTTGAGA CATCTCAGAA AAATTTGGAA GTAGCTCTTA CTCACAAAGA TGATAATATT 
AATGCTTTGA CTAATTGCAT TACCCAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG 
GATCAGAGTA AAGGAGGAAG TGAGTTAGAT GAGTTAGCAA ATGGAGAAGT AGGAGGTGAC 
CGGAGTGAGA AAATGAAAAA TCAGATTAAG CAGATGATGG ACATTTCTCG GACACAAACT 
GCAATATCAG TAGTTGAAGA GGATCTAAAA CTTCTGCAGA TTAAGCTTAG AGCCTCAATG 
TCTACTAAAT GCAACCTGGA AGACCAAATA AAGAAATTAG AAGATGACCG TGATTCACTG 
CAGTCTGCCA AAGCCGGCCT GGAGGACGAA TGCAAAACCC TGAGGCAAAA AGTGGAGATT 
CTGAACGAAC TCTATCAACA GAAGGAAATG GCTCTGCAAA AGAAACTGAG TCAGGAAGAG 
TATGAGCGGC AGGAGAGAGA GCAGCGGCTG TCAGCTGCAG ACGAGAAGGC GCTTCTGGCT 
GCGGAGGAAG TAAAAACGTA CAAGCGTAGA ATCGAAGAGA TGGAGGATGA GTTGCAGAAG 
ACAGAGCGCT CGTTTAAAAA CCAGATTGCG ACTCATGAGA AGAAAGCTCA TGATAACTGG 
CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCCGAGGAGA AGAGGGAAGC TGCCAACTTG 
AGACACAAGT TACTGGAATT AACCCAGAAG ATGGCAATGC TGCAAGAGGA GCCTGTGATT 
GTGAAGCCGA GGCCGGGCCG ACCCAGCGCC CAGAACCCTC CGTGGAGAGG TCCTTTGAGC 
CAGAACGGCT CTTTTGGTCC ATCCCCTGTG AGTGGGGGGG AGTGCTCCCC ACCTCCCACG 
GCTGACCTGC CTGCGAGACC GCTCTCTGCT ACGCTGAATC GAAGAGAGAT GCCTAGAGGT 
GAATTTGGGT CAGTGGACGG GCCTCTACCT CGTCCTCGTT GGTCATCTGA GGCATCTGGG 
AAACCCTCTG CCTCTGATCT GGGATCTGGT GCAGCTCCCA CGATGAACAG CAGCTCGAGA 
AGCTCTTCCC CTACCAAGGT GACGGATGAA GGCAAGCAAA CTGTTCCCCA AGACCCCGAA 
GGCCCTTCAG TTCCCAGCAG TTCACCTGTG GCTGAGCAGT CGGTAGTAGT TCCTATGGCT 
GCAAAAGGGC CGCCTCCTTT CCCAGGGGTG CCCCTCATGG GCTCCCCCGT GGGCGGCCCT 
CTACCACCAC CCATTCGCTA CGGACCACCC CCTCAACTTT GCGGGCCTTT CGGGCCTCGG 
CCGCTTCCTC CACCATTTGG TCCTGCTTTA CGTCCACCAC TAGGCTTAAG AGAATACGCA 
CCAGGCATTC CACCTGGAAA ACGGGACCTG TCTCTTGATC CTCGAGAATT TTTACCGGGA 
CATGCACCTT TTAGACCTTT AGGCTCTTTT GGCCCAAGAG AGTACTTTAT TCCCGGTACC 
CGACTACCAC CCCCTGCCCA TGGTCCCCAG GATTATCCAC CTCCACCTGC TGCAAGAGAC 
TTACTGCCTT CTGGCTCCAG AGACGAGCCT CCACCTGCCT CCCAGGGCAG TAGCCAGGAC 
TGTTCACAGG CTTTAAAACA GAGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022271164.1
CDS	165..2594
Translation	MDSVPATVPSVTASPGDSELLGPLSVLYAALIAKLLELVATLPDDVQPGPDFYGLPWKPVLLTAFLGIGSFAVFFWKTVLVVKSRVYQVTEQQISEKLKNIMKENAELVQKLSNYEQKIKESKKHVQETKKQNIILSDEAIKFKDKIKKLEETNEILDDTAKNLRVMLESEREQNAKNQDLILENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIKSFETSQKNLEVALTHKDDNINALTNCITQLNRLDCESESEDQSKGGSELDELANGEVGGDRSEKMKNQIKQMMDISRTQTAISVVEEDLKLLQIKLRASMSTKCNLEDQIKKLEDDRDSLQSAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKALLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPRPGRPSAQNPPWRGPLSQNGSFGPSPVSGGECSPPPTADLPARPLSATLNRREMPRGEFGSVDGPLPRPRWSSEASGKPSASDLGSGAAPTMNSSSRSSSPTKVTDEGKQTVPQDPEGPSVPSSSPVAEQSVVVPMAAKGPPPFPGVPLMGSPVGGPLPPPIRYGPPPQLCGPFGPRPLPPPFGPALRPPLGLREYAPGIPPGKRDLSLDPREFLPGHAPFRPLGSFGPREYFIPGTRLPPPAHGPQDYPPPPAARDLLPSGSRDEPPPASQGSSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_022415456.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022415456.1

ORF Insert Sequence:

ATGGACTCCG TTCCCGCCAC TGTGCCTTCT GTCACCGCCT CCCCGGGGGA CTCGGAGCTC 
CTGGGACCCC TGTCGGTGCT CTACGCGGCC CTCATAGCCA AGCTGCTGGA GTTAGTTGCT 
ACATTGCCTG ATGACGTTCA GCCTGGGCCT GATTTTTATG GCTTGCCATG GAAGCCTGTA 
CTTCTCACTG CCTTCTTGGG AATTGGTTCA TTTGCGGTCT TCTTCTGGAA AACTGTCCTT 
GTTGTAAAGA GTAGAGTCTA TCAAGTCACT GAACAACAAA TTTCTGAGAA GTTGAAGAAC 
ATCATGAAAG AAAATGCAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATTAAG 
GAATCAAAAA AACATGTTCA AGAAACCAAG AAACAAAATA TAATTCTGTC TGATGAAGCA 
ATTAAATTTA AGGATAAAAT CAAGAAACTT GAAGAAACAA ATGAAATTCT GGACGATACA 
GCTAAAAATC TGCGTGTTAT GTTAGAATCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC 
TTGATACTGG AAAACAAGAA ATCTATAGAG AAGCTGAAGG ATGTTATTTC AGTGAATGCC 
TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAGGTG 
AAGTCTGAAT GCCATCGAGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA GAAGGAACAG 
TTGCAGCAAG AAATCAAAGA TTGGAGCAAA TCACACGCTG AGCTCAGTGA GCAAATCAAA 
TCATTTGAGA CATCTCAGAA AAATTTGGAA GTAGCTCTTA CTCACAAAGA TGATAATATT 
AATGCTTTGA CTAATTGCAT TACCCAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG 
GATCAGAGTA AAGGAGGAAG TGAGTTAGAT GAGTTAGCAA ATGGAGAAGT AGGAGGTGAC 
CGGAGTGAGA AAATGAAAAA TCAGATTAAG CAGATGATGG ACATTTCTCG GACACAAACT 
GCAATATCAG TAGTTGAAGA GGATCTAAAA CTTCTGCAGA TTAAGCTTAG AGCCTCAATG 
TCTACTAAAT GCAACCTGGA AGACCAAATA AAGAAATTAG AAGATGACCG TGATTCACTG 
CAGTCTGCCA AAGCCGGCCT GGAGGACGAA TGCAAAACCC TGAGGCAAAA AGTGGAGATT 
CTGAACGAAC TCTATCAACA GAAGGAAATG GCTCTGCAAA AGAAACTGAG TCAGGAAGAG 
TATGAGCGGC AGGAGAGAGA GCAGCGGCTG TCAGCTGCAG ACGAGAAGGC GCTTCTGGCT 
GCGGAGGAAG TAAAAACGTA CAAGCGTAGA ATCGAAGAGA TGGAGGATGA GTTGCAGAAG 
ACAGAGCGCT CGTTTAAAAA CCAGATTGCG ACTCATGAGA AGAAAGCTCA TGATAACTGG 
CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCCGAGGAGA AGAGGGAAGC TGCCAACTTG 
AGACACAAGT TACTGGAATT AACCCAGAAG ATGGCAATGC TGCAAGAGGA GCCTGTGATT 
GTGAAGCCGA GGCCGGGCCG ACCCAGCGCC CAGAACCCTC CGTGGAGAGG TCCTTTGAGC 
CAGAACGGCT CTTTTGGTCC ATCCCCTGTG AGTGGGGGGG AGTGCTCCCC ACCTCCCACG 
GCTGACCTGC CTGCGAGACC GCTCTCTGCT ACGCTGAATC GAAGAGAGAT GCCTAGAGGT 
GAATTTGGGT CAGTGGACGG GCCTCTACCT CGTCCTCGTT GGTCATCTGA GGCATCTGGG 
AAACCCTCTG CCTCTGATCT GGGATCTGGT GCAGCTCCCA CGATGAACAG CAGCTCGAGA 
AGCTCTTCCC CTACCAAGGT GACGGATGAA GGCAAGCAAA CTGTTCCCCA AGACCCCGAA 
GGCCCTTCAG TTCCCAGCAG TTCACCTGTG GCTGAGCAGT CGGTAGTAGT TCCTATGGCT 
GCAAAAGGGC CGCCTCCTTT CCCAGGGGTG CCCCTCATGG GCTCCCCCGT GGGCGGCCCT 
CTACCACCAC CCATTCGCTA CGGACCACCC CCTCAACTTT GCGGGCCTTT CGGGCCTCGG 
CCGCTTCCTC CACCATTTGG TCCTGCTTTA CGTCCACCAC TAGGCTTAAG AGAATACGCA 
CCAGGCATTC CACCTGGAAA ACGGGACCTG TCTCTTGATC CTCGAGAATT TTTACCGGGA 
CATGCACCTT TTAGACCTTT AGGCTCTTTT GGCCCAAGAG AGTACTTTAT TCCCGGTACC 
CGACTACCAC CCCCTGCCCA TGGTCCCCAG GATTATCCAC CTCCACCTGC TGCAAGAGAC 
TTACTGCCTT CTGGCTCCAG AGACGAGCCT CCACCTGCCT CCCAGGGCAG TAGCCAGGAC 
TGTTCACAGG CTTTAAAACA GAGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_014111164.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966639.1 Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OCe45302
Clone ID Related Accession (Same CDS sequence)	XM_014111164.2 , XM_014111164.1
Accession Version	XM_014111164.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transport and Golgi organization protein 1 homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014111164.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCG TTCCCGCCAC TGTGCCTTCT GTCACCGCCT CCCCGGGGGA CTCGGAGCTC 
CTGGGACCCC TGTCGGTGCT CTACGCGGCC CTCATAGCCA AGCTGCTGGA GTTAGTTGCT 
ACATTGCCTG ATGACGTTCA GCCTGGGCCT GATTTTTATG GCTTGCCATG GAAGCCTGTA 
CTTCTCACTG CCTTCTTGGG AATTGGTTCA TTTGCGGTCT TCTTCTGGAA AACTGTCCTT 
GTTGTAAAGA GTAGAGTCTA TCAAGTCACT GAACAACAAA TTTCTGAGAA GTTGAAGAAC 
ATCATGAAAG AAAATGCAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATTAAG 
GAATCAAAAA AACATGTTCA AGAAACCAAG AAACAAAATA TAATTCTGTC TGATGAAGCA 
ATTAAATTTA AGGATAAAAT CAAGAAACTT GAAGAAACAA ATGAAATTCT GGACGATACA 
GCTAAAAATC TGCGTGTTAT GTTAGAATCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC 
TTGATACTGG AAAACAAGAA ATCTATAGAG AAGCTGAAGG ATGTTATTTC AGTGAATGCC 
TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAGGTG 
AAGTCTGAAT GCCATCGAGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA GAAGGAACAG 
TTGCAGCAAG AAATCAAAGA TTGGAGCAAA TCACACGCTG AGCTCAGTGA GCAAATCAAA 
TCATTTGAGA CATCTCAGAA AAATTTGGAA GTAGCTCTTA CTCACAAAGA TGATAATATT 
AATGCTTTGA CTAATTGCAT TACCCAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG 
GATCAGAGTA AAGGAGGAAG TGAGTTAGAT GAGTTAGCAA ATGGAGAAGT AGGAGGTGAC 
CGGAGTGAGA AAATGAAAAA TCAGATTAAG CAGATGATGG ACATTTCTCG GACACAAACT 
GCAATATCAG TAGTTGAAGA GGATCTAAAA CTTCTGCAGA TTAAGCTTAG AGCCTCAATG 
TCTACTAAAT GCAACCTGGA AGACCAAATA AAGAAATTAG AAGATGACCG TGATTCACTG 
CAGTCTGCCA AAGCCGGCCT GGAGGACGAA TGCAAAACCC TGAGGCAAAA AGTGGAGATT 
CTGAACGAAC TCTATCAACA GAAGGAAATG GCTCTGCAAA AGAAACTGAG TCAGGAAGAG 
TATGAGCGGC AGGAGAGAGA GCAGCGGCTG TCAGCTGCAG ACGAGAAGGC GCTTCTGGCT 
GCGGAGGAAG TAAAAACGTA CAAGCGTAGA ATCGAAGAGA TGGAGGATGA GTTGCAGAAG 
ACAGAGCGCT CGTTTAAAAA CCAGATTGCG ACTCATGAGA AGAAAGCTCA TGATAACTGG 
CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCCGAGGAGA AGAGGGAAGC TGCCAACTTG 
AGACACAAGT TACTGGAATT AACCCAGAAG ATGGCAATGC TGCAAGAGGA GCCTGTGATT 
GTGAAGCCGA GGCCGGGCCG ACCCAGCGCC CAGAACCCTC CGTGGAGAGG TCCTTTGAGC 
CAGAACGGCT CTTTTGGTCC ATCCCCTGTG AGTGGGGGGG AGTGCTCCCC ACCTCCCACG 
GCTGACCTGC CTGCGAGACC GCTCTCTGCT ACGCTGAATC GAAGAGAGAT GCCTAGAGGT 
GAATTTGGGT CAGTGGACGG GCCTCTACCT CGTCCTCGTT GGTCATCTGA GGCATCTGGG 
AAACCCTCTG CCTCTGATCT GGGATCTGGT GCAGCTCCCA CGATGAACAG CAGCTCGAGA 
AGCTCTTCCC CTACCAAGGT GACGGATGAA GGCAAGGTTC CTATGGCTGC AAAAGGGCCG 
CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC 
ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA 
CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA 
CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT 
AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC 
CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT 
GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT 
TTAAAACAGA GCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966639.1
CDS	1..2358
Translation	MDSVPATVPSVTASPGDSELLGPLSVLYAALIAKLLELVATLPDDVQPGPDFYGLPWKPVLLTAFLGIGSFAVFFWKTVLVVKSRVYQVTEQQISEKLKNIMKENAELVQKLSNYEQKIKESKKHVQETKKQNIILSDEAIKFKDKIKKLEETNEILDDTAKNLRVMLESEREQNAKNQDLILENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIKSFETSQKNLEVALTHKDDNINALTNCITQLNRLDCESESEDQSKGGSELDELANGEVGGDRSEKMKNQIKQMMDISRTQTAISVVEEDLKLLQIKLRASMSTKCNLEDQIKKLEDDRDSLQSAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKALLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPRPGRPSAQNPPWRGPLSQNGSFGPSPVSGGECSPPPTADLPARPLSATLNRREMPRGEFGSVDGPLPRPRWSSEASGKPSASDLGSGAAPTMNSSSRSSSPTKVTDEGKVPMAAKGPPPFPGVPLMGSPVGGPLPPPIRYGPPPQLCGPFGPRPLPPPFGPALRPPLGLREYAPGIPPGKRDLSLDPREFLPGHAPFRPLGSFGPREYFIPGTRLPPPAHGPQDYPPPPAARDLLPSGSRDEPPPASQGSSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_014111164.2
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111164.2

ORF Insert Sequence:

ATGGACTCCG TTCCCGCCAC TGTGCCTTCT GTCACCGCCT CCCCGGGGGA CTCGGAGCTC 
CTGGGACCCC TGTCGGTGCT CTACGCGGCC CTCATAGCCA AGCTGCTGGA GTTAGTTGCT 
ACATTGCCTG ATGACGTTCA GCCTGGGCCT GATTTTTATG GCTTGCCATG GAAGCCTGTA 
CTTCTCACTG CCTTCTTGGG AATTGGTTCA TTTGCGGTCT TCTTCTGGAA AACTGTCCTT 
GTTGTAAAGA GTAGAGTCTA TCAAGTCACT GAACAACAAA TTTCTGAGAA GTTGAAGAAC 
ATCATGAAAG AAAATGCAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATTAAG 
GAATCAAAAA AACATGTTCA AGAAACCAAG AAACAAAATA TAATTCTGTC TGATGAAGCA 
ATTAAATTTA AGGATAAAAT CAAGAAACTT GAAGAAACAA ATGAAATTCT GGACGATACA 
GCTAAAAATC TGCGTGTTAT GTTAGAATCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC 
TTGATACTGG AAAACAAGAA ATCTATAGAG AAGCTGAAGG ATGTTATTTC AGTGAATGCC 
TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAGGTG 
AAGTCTGAAT GCCATCGAGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA GAAGGAACAG 
TTGCAGCAAG AAATCAAAGA TTGGAGCAAA TCACACGCTG AGCTCAGTGA GCAAATCAAA 
TCATTTGAGA CATCTCAGAA AAATTTGGAA GTAGCTCTTA CTCACAAAGA TGATAATATT 
AATGCTTTGA CTAATTGCAT TACCCAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG 
GATCAGAGTA AAGGAGGAAG TGAGTTAGAT GAGTTAGCAA ATGGAGAAGT AGGAGGTGAC 
CGGAGTGAGA AAATGAAAAA TCAGATTAAG CAGATGATGG ACATTTCTCG GACACAAACT 
GCAATATCAG TAGTTGAAGA GGATCTAAAA CTTCTGCAGA TTAAGCTTAG AGCCTCAATG 
TCTACTAAAT GCAACCTGGA AGACCAAATA AAGAAATTAG AAGATGACCG TGATTCACTG 
CAGTCTGCCA AAGCCGGCCT GGAGGACGAA TGCAAAACCC TGAGGCAAAA AGTGGAGATT 
CTGAACGAAC TCTATCAACA GAAGGAAATG GCTCTGCAAA AGAAACTGAG TCAGGAAGAG 
TATGAGCGGC AGGAGAGAGA GCAGCGGCTG TCAGCTGCAG ACGAGAAGGC GCTTCTGGCT 
GCGGAGGAAG TAAAAACGTA CAAGCGTAGA ATCGAAGAGA TGGAGGATGA GTTGCAGAAG 
ACAGAGCGCT CGTTTAAAAA CCAGATTGCG ACTCATGAGA AGAAAGCTCA TGATAACTGG 
CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCCGAGGAGA AGAGGGAAGC TGCCAACTTG 
AGACACAAGT TACTGGAATT AACCCAGAAG ATGGCAATGC TGCAAGAGGA GCCTGTGATT 
GTGAAGCCGA GGCCGGGCCG ACCCAGCGCC CAGAACCCTC CGTGGAGAGG TCCTTTGAGC 
CAGAACGGCT CTTTTGGTCC ATCCCCTGTG AGTGGGGGGG AGTGCTCCCC ACCTCCCACG 
GCTGACCTGC CTGCGAGACC GCTCTCTGCT ACGCTGAATC GAAGAGAGAT GCCTAGAGGT 
GAATTTGGGT CAGTGGACGG GCCTCTACCT CGTCCTCGTT GGTCATCTGA GGCATCTGGG 
AAACCCTCTG CCTCTGATCT GGGATCTGGT GCAGCTCCCA CGATGAACAG CAGCTCGAGA 
AGCTCTTCCC CTACCAAGGT GACGGATGAA GGCAAGGTTC CTATGGCTGC AAAAGGGCCG 
CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC 
ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA 
CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA 
CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT 
AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC 
CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT 
GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT 
TTAAAACAGA GCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_014111164.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966639.1 Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OCe45302
Clone ID Related Accession (Same CDS sequence)	XM_014111164.2 , XM_014111164.1
Accession Version	XM_014111164.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	melanoma inhibitory activity protein 3 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACTCCG TTCCCGCCAC TGTGCCTTCT GTCACCGCCT CCCCGGGGGA CTCGGAGCTC 
CTGGGACCCC TGTCGGTGCT CTACGCGGCC CTCATAGCCA AGCTGCTGGA GTTAGTTGCT 
ACATTGCCTG ATGACGTTCA GCCTGGGCCT GATTTTTATG GCTTGCCATG GAAGCCTGTA 
CTTCTCACTG CCTTCTTGGG AATTGGTTCA TTTGCGGTCT TCTTCTGGAA AACTGTCCTT 
GTTGTAAAGA GTAGAGTCTA TCAAGTCACT GAACAACAAA TTTCTGAGAA GTTGAAGAAC 
ATCATGAAAG AAAATGCAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATTAAG 
GAATCAAAAA AACATGTTCA AGAAACCAAG AAACAAAATA TAATTCTGTC TGATGAAGCA 
ATTAAATTTA AGGATAAAAT CAAGAAACTT GAAGAAACAA ATGAAATTCT GGACGATACA 
GCTAAAAATC TGCGTGTTAT GTTAGAATCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC 
TTGATACTGG AAAACAAGAA ATCTATAGAG AAGCTGAAGG ATGTTATTTC AGTGAATGCC 
TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAGGTG 
AAGTCTGAAT GCCATCGAGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA GAAGGAACAG 
TTGCAGCAAG AAATCAAAGA TTGGAGCAAA TCACACGCTG AGCTCAGTGA GCAAATCAAA 
TCATTTGAGA CATCTCAGAA AAATTTGGAA GTAGCTCTTA CTCACAAAGA TGATAATATT 
AATGCTTTGA CTAATTGCAT TACCCAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG 
GATCAGAGTA AAGGAGGAAG TGAGTTAGAT GAGTTAGCAA ATGGAGAAGT AGGAGGTGAC 
CGGAGTGAGA AAATGAAAAA TCAGATTAAG CAGATGATGG ACATTTCTCG GACACAAACT 
GCAATATCAG TAGTTGAAGA GGATCTAAAA CTTCTGCAGA TTAAGCTTAG AGCCTCAATG 
TCTACTAAAT GCAACCTGGA AGACCAAATA AAGAAATTAG AAGATGACCG TGATTCACTG 
CAGTCTGCCA AAGCCGGCCT GGAGGACGAA TGCAAAACCC TGAGGCAAAA AGTGGAGATT 
CTGAACGAAC TCTATCAACA GAAGGAAATG GCTCTGCAAA AGAAACTGAG TCAGGAAGAG 
TATGAGCGGC AGGAGAGAGA GCAGCGGCTG TCAGCTGCAG ACGAGAAGGC GCTTCTGGCT 
GCGGAGGAAG TAAAAACGTA CAAGCGTAGA ATCGAAGAGA TGGAGGATGA GTTGCAGAAG 
ACAGAGCGCT CGTTTAAAAA CCAGATTGCG ACTCATGAGA AGAAAGCTCA TGATAACTGG 
CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCCGAGGAGA AGAGGGAAGC TGCCAACTTG 
AGACACAAGT TACTGGAATT AACCCAGAAG ATGGCAATGC TGCAAGAGGA GCCTGTGATT 
GTGAAGCCGA GGCCGGGCCG ACCCAGCGCC CAGAACCCTC CGTGGAGAGG TCCTTTGAGC 
CAGAACGGCT CTTTTGGTCC ATCCCCTGTG AGTGGGGGGG AGTGCTCCCC ACCTCCCACG 
GCTGACCTGC CTGCGAGACC GCTCTCTGCT ACGCTGAATC GAAGAGAGAT GCCTAGAGGT 
GAATTTGGGT CAGTGGACGG GCCTCTACCT CGTCCTCGTT GGTCATCTGA GGCATCTGGG 
AAACCCTCTG CCTCTGATCT GGGATCTGGT GCAGCTCCCA CGATGAACAG CAGCTCGAGA 
AGCTCTTCCC CTACCAAGGT GACGGATGAA GGCAAGGTTC CTATGGCTGC AAAAGGGCCG 
CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC 
ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA 
CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA 
CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT 
AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC 
CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT 
GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT 
TTAAAACAGA GCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966639.1
CDS	167..2524
Translation	MDSVPATVPSVTASPGDSELLGPLSVLYAALIAKLLELVATLPDDVQPGPDFYGLPWKPVLLTAFLGIGSFAVFFWKTVLVVKSRVYQVTEQQISEKLKNIMKENAELVQKLSNYEQKIKESKKHVQETKKQNIILSDEAIKFKDKIKKLEETNEILDDTAKNLRVMLESEREQNAKNQDLILENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIKSFETSQKNLEVALTHKDDNINALTNCITQLNRLDCESESEDQSKGGSELDELANGEVGGDRSEKMKNQIKQMMDISRTQTAISVVEEDLKLLQIKLRASMSTKCNLEDQIKKLEDDRDSLQSAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKALLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPRPGRPSAQNPPWRGPLSQNGSFGPSPVSGGECSPPPTADLPARPLSATLNRREMPRGEFGSVDGPLPRPRWSSEASGKPSASDLGSGAAPTMNSSSRSSSPTKVTDEGKVPMAAKGPPPFPGVPLMGSPVGGPLPPPIRYGPPPQLCGPFGPRPLPPPFGPALRPPLGLREYAPGIPPGKRDLSLDPREFLPGHAPFRPLGSFGPREYFIPGTRLPPPAHGPQDYPPPPAARDLLPSGSRDEPPPASQGSSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_014111164.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111164.1

ORF Insert Sequence:

ATGGACTCCG TTCCCGCCAC TGTGCCTTCT GTCACCGCCT CCCCGGGGGA CTCGGAGCTC 
CTGGGACCCC TGTCGGTGCT CTACGCGGCC CTCATAGCCA AGCTGCTGGA GTTAGTTGCT 
ACATTGCCTG ATGACGTTCA GCCTGGGCCT GATTTTTATG GCTTGCCATG GAAGCCTGTA 
CTTCTCACTG CCTTCTTGGG AATTGGTTCA TTTGCGGTCT TCTTCTGGAA AACTGTCCTT 
GTTGTAAAGA GTAGAGTCTA TCAAGTCACT GAACAACAAA TTTCTGAGAA GTTGAAGAAC 
ATCATGAAAG AAAATGCAGA ACTTGTACAA AAATTGTCAA ATTATGAACA GAAGATTAAG 
GAATCAAAAA AACATGTTCA AGAAACCAAG AAACAAAATA TAATTCTGTC TGATGAAGCA 
ATTAAATTTA AGGATAAAAT CAAGAAACTT GAAGAAACAA ATGAAATTCT GGACGATACA 
GCTAAAAATC TGCGTGTTAT GTTAGAATCT GAGAGAGAAC AGAATGCCAA GAATCAGGAC 
TTGATACTGG AAAACAAGAA ATCTATAGAG AAGCTGAAGG ATGTTATTTC AGTGAATGCC 
TCAGAATTTT CAGAGGTTCA AATTGCCCTT AATGAAGCTA AACTTAGTGA AGAGAAGGTG 
AAGTCTGAAT GCCATCGAGT TCAAGAAGAA AATGCTAGGC TTAAGAAGAA GAAGGAACAG 
TTGCAGCAAG AAATCAAAGA TTGGAGCAAA TCACACGCTG AGCTCAGTGA GCAAATCAAA 
TCATTTGAGA CATCTCAGAA AAATTTGGAA GTAGCTCTTA CTCACAAAGA TGATAATATT 
AATGCTTTGA CTAATTGCAT TACCCAGTTG AATCGGTTAG ATTGTGAATC TGAATCTGAG 
GATCAGAGTA AAGGAGGAAG TGAGTTAGAT GAGTTAGCAA ATGGAGAAGT AGGAGGTGAC 
CGGAGTGAGA AAATGAAAAA TCAGATTAAG CAGATGATGG ACATTTCTCG GACACAAACT 
GCAATATCAG TAGTTGAAGA GGATCTAAAA CTTCTGCAGA TTAAGCTTAG AGCCTCAATG 
TCTACTAAAT GCAACCTGGA AGACCAAATA AAGAAATTAG AAGATGACCG TGATTCACTG 
CAGTCTGCCA AAGCCGGCCT GGAGGACGAA TGCAAAACCC TGAGGCAAAA AGTGGAGATT 
CTGAACGAAC TCTATCAACA GAAGGAAATG GCTCTGCAAA AGAAACTGAG TCAGGAAGAG 
TATGAGCGGC AGGAGAGAGA GCAGCGGCTG TCAGCTGCAG ACGAGAAGGC GCTTCTGGCT 
GCGGAGGAAG TAAAAACGTA CAAGCGTAGA ATCGAAGAGA TGGAGGATGA GTTGCAGAAG 
ACAGAGCGCT CGTTTAAAAA CCAGATTGCG ACTCATGAGA AGAAAGCTCA TGATAACTGG 
CTCAAAGCTC GTGCTGCAGA AAGAGCTATA GCCGAGGAGA AGAGGGAAGC TGCCAACTTG 
AGACACAAGT TACTGGAATT AACCCAGAAG ATGGCAATGC TGCAAGAGGA GCCTGTGATT 
GTGAAGCCGA GGCCGGGCCG ACCCAGCGCC CAGAACCCTC CGTGGAGAGG TCCTTTGAGC 
CAGAACGGCT CTTTTGGTCC ATCCCCTGTG AGTGGGGGGG AGTGCTCCCC ACCTCCCACG 
GCTGACCTGC CTGCGAGACC GCTCTCTGCT ACGCTGAATC GAAGAGAGAT GCCTAGAGGT 
GAATTTGGGT CAGTGGACGG GCCTCTACCT CGTCCTCGTT GGTCATCTGA GGCATCTGGG 
AAACCCTCTG CCTCTGATCT GGGATCTGGT GCAGCTCCCA CGATGAACAG CAGCTCGAGA 
AGCTCTTCCC CTACCAAGGT GACGGATGAA GGCAAGGTTC CTATGGCTGC AAAAGGGCCG 
CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC 
ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA 
CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA 
CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT 
AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC 
CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT 
GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT 
TTAAAACAGA GCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_022415455.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022271163.1 Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCe285056
Clone ID Related Accession (Same CDS sequence)	XM_022415455.1
Accession Version	XM_022415455.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transport and Golgi organization protein 1 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT 
GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA 
GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA 
GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG 
GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG 
ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA 
CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA 
GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA 
GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA 
CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT 
GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA 
GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG 
ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT 
GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA 
GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA 
GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT 
GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA 
AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT 
ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG 
GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG 
AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT 
GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA 
ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC 
GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT 
GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT 
GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT 
GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA 
GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA 
CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA 
GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA 
CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA 
GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA 
GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG 
GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG 
GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA 
CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC 
GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG 
CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC 
AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG 
GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT 
CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT 
CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC 
AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC 
TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC 
CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC 
TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC 
TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG 
CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT 
GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA 
GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA 
GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC 
ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT 
GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC 
AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC 
CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT 
CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA 
GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA 
AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT 
CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT 
GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC 
TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT 
AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA 
AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA 
CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG 
GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG 
CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA 
AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA 
GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC 
CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA 
ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA 
TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT 
AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA 
GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA 
ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA 
GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC 
AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA 
GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC 
TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG 
GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA 
AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG 
TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT 
GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA 
CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG 
CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT 
TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT 
GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGATCTG 
GGATCTGGTG CAGCTCCCAC GATGAACAGC AGCTCGAGAA GCTCTTCCCC TACCAAGGTG 
ACGGATGAAG GCAAGGTTCC TATGGCTGCA AAAGGGCCGC CTCCTTTCCC AGGGGTGCCC 
CTCATGGGCT CCCCCGTGGG CGGCCCTCTA CCACCACCCA TTCGCTACGG ACCACCCCCT 
CAACTTTGCG GGCCTTTCGG GCCTCGGCCG CTTCCTCCAC CATTTGGTCC TGCTTTACGT 
CCACCACTAG GCTTAAGAGA ATACGCACCA GGCATTCCAC CTGGAAAACG GGACCTGTCT 
CTTGATCCTC GAGAATTTTT ACCGGGACAT GCACCTTTTA GACCTTTAGG CTCTTTTGGC 
CCAAGAGAGT ACTTTATTCC CGGTACCCGA CTACCACCCC CTGCCCATGG TCCCCAGGAT 
TATCCACCTC CACCTGCTGC AAGAGACTTA CTGCCTTCTG GCTCCAGAGA CGAGCCTCCA 
CCTGCCTCCC AGGGCAGTAG CCAGGACTGT TCACAGGCTT TAAAACAGAG CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022271163.1
CDS	105..5861
Translation	MEFGLFIFKLVLFENRFHSWKSLYPGNTPCTGSEEASDPCEAIPRVLMYRGEALEDFIGPDCRFVSFKKGDAVYVYYKLAGRPPEVWAGSVGRIFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFDNYNVEELLGFLELHNSATEESEKAIEETQYEEDTPPEVLEESDLELEPVEVNSEESESVASENTKELKERSGAQKNHPHVNSPADHAQGDPSSFEPFEEMLQDKLKVPESENNKTSNGSQVSNEQQTIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDAEYHIVGKDEEENEDNFDELPLLTFAHGGEDVKTPVKSGVEKYSAESEQSSNEEDKVEAPQPPGIKNYDKDILTTWEDTVFFTEDDSVTSMDLGSSESQEGKEGDDAFIPESKWRKPRSAADYIDPKKAEDGLLPTANDVGTKTDNDEDLEIHIAGKGRGAKEPKPGLAQAEMGPEEEKQEGMTTHGSTHSGDLGSLPSTGKGKEMLESAFDNKEDDLKGAAIHISKGMLHEEKPGEQISEGGSEREPLPQATGGQVNEEKPDQESVGIAAPSGGGQHNASRDRVEDVHALAGLKPHTPSVEHPKEEFREERLLKTQNQPRPSPLDGVGLPGELKEEGPIWGRNLSWPQGGEVAAAHSKQMDEKGRLAEEEVREDSSAEELAPQQPKAGSQEVEPTGRPGSPEVPGVHTEKPEAEDDHSPEELLEDENALSAKQAKEKSPGIQGRWLDVNLQSPSRAPLGVVNTDAEMEGNKEEASTILEIERKGETFGKEVEPGGRGREAGSGVVETESPLAGAKAQTASGGSDSPDKKNRTPGLGEMFQNQDSDYFKEDSAEKSAQKPGVEELSEEDQDFERPADTQSQGPAPEEQEGGPLRGTPPTAVRPMRSDYVKDLPIIGSFFKEQQSLQRFQKYFDVHELEAMFHEMSLKLKLAQQESLPYNVEKVLDKVFRASESQILSTAEKMLDTRVAENRDLGMMESNIFEEAAVLDDIQDLIYFVRYRHSTMEETAPLATGQPPQEGWGGTLEGMQPHLEDNFPQGHTKDNFPQDHIEDSFPQDHTEDSFPQDHTEDNFPQDHTEDLQIQILKEPSQLDQPVVGDMGASEVSQQPNTEKDGDPGIITTESSPVDADDAKKQLQTNAEEPASVTPLENAILLIYSFIFYLTKTLVATLPDDVQPGPDFYGLPWKPVLLTAFLGIGSFAVFFWKTVLVVKSRVYQVTEQQISEKLKNIMKENAELVQKLSNYEQKIKESKKHVQETKKQNIILSDEAIKFKDKIKKLEETNEILDDTAKNLRVMLESEREQNAKNQDLILENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIKSFETSQKNLEVALTHKDDNINALTNCITQLNRLDCESESEDQSKGGSELDELANGEVGGDRSEKMKNQIKQMMDISRTQTAISVVEEDLKLLQIKLRASMSTKCNLEDQIKKLEDDRDSLQSAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKALLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPRPGRPSAQNPPWRGPLSQNGSFGPSPVSGGECSPPPTADLPARPLSATLNRREMPRGEFDLGSGAAPTMNSSSRSSSPTKVTDEGKVPMAAKGPPPFPGVPLMGSPVGGPLPPPIRYGPPPQLCGPFGPRPLPPPFGPALRPPLGLREYAPGIPPGKRDLSLDPREFLPGHAPFRPLGSFGPREYFIPGTRLPPPAHGPQDYPPPPAARDLLPSGSRDEPPPASQGSSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_022415455.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022415455.1

ORF Insert Sequence:

ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT 
GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA 
GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA 
GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG 
GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG 
ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA 
CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA 
GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA 
GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA 
CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT 
GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA 
GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG 
ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT 
GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA 
GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA 
GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT 
GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA 
AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT 
ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG 
GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG 
AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT 
GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA 
ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC 
GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT 
GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT 
GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT 
GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA 
GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA 
CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA 
GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA 
CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA 
GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA 
GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG 
GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG 
GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA 
CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC 
GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG 
CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC 
AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG 
GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT 
CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT 
CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC 
AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC 
TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC 
CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC 
TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC 
TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG 
CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT 
GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA 
GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA 
GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC 
ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT 
GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC 
AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC 
CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT 
CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA 
GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA 
AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT 
CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT 
GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC 
TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT 
AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA 
AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA 
CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG 
GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG 
CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA 
AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA 
GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC 
CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA 
ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA 
TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT 
AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA 
GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA 
ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA 
GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC 
AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA 
GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC 
TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG 
GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA 
AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG 
TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT 
GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA 
CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG 
CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT 
TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT 
GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGATCTG 
GGATCTGGTG CAGCTCCCAC GATGAACAGC AGCTCGAGAA GCTCTTCCCC TACCAAGGTG 
ACGGATGAAG GCAAGGTTCC TATGGCTGCA AAAGGGCCGC CTCCTTTCCC AGGGGTGCCC 
CTCATGGGCT CCCCCGTGGG CGGCCCTCTA CCACCACCCA TTCGCTACGG ACCACCCCCT 
CAACTTTGCG GGCCTTTCGG GCCTCGGCCG CTTCCTCCAC CATTTGGTCC TGCTTTACGT 
CCACCACTAG GCTTAAGAGA ATACGCACCA GGCATTCCAC CTGGAAAACG GGACCTGTCT 
CTTGATCCTC GAGAATTTTT ACCGGGACAT GCACCTTTTA GACCTTTAGG CTCTTTTGGC 
CCAAGAGAGT ACTTTATTCC CGGTACCCGA CTACCACCCC CTGCCCATGG TCCCCAGGAT 
TATCCACCTC CACCTGCTGC AAGAGACTTA CTGCCTTCTG GCTCCAGAGA CGAGCCTCCA 
CCTGCCTCCC AGGGCAGTAG CCAGGACTGT TCACAGGCTT TAAAACAGAG CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_005640835.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005640892.1 Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X1, mRNA.

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CloneID	OCe31714
Clone ID Related Accession (Same CDS sequence)	XM_005640835.2 , XM_005640835.3
Accession Version	XM_005640835.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	melanoma inhibitory activity protein 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640835.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT 
GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA 
GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA 
GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG 
GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG 
ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA 
CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA 
GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA 
GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA 
CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT 
GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA 
GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG 
ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT 
GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA 
GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA 
GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT 
GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA 
AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT 
ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG 
GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG 
AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT 
GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA 
ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC 
GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT 
GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT 
GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT 
GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA 
GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA 
CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA 
GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA 
CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA 
GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA 
GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG 
GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG 
GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA 
CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC 
GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG 
CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC 
AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG 
GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT 
CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT 
CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC 
AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC 
TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC 
CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC 
TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC 
TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG 
CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT 
GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA 
GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA 
GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC 
ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT 
GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC 
AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC 
CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT 
CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA 
GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA 
AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT 
CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT 
GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC 
TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT 
AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA 
AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA 
CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG 
GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG 
CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA 
AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA 
GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC 
CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA 
ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA 
TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT 
AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA 
GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA 
ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA 
GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC 
AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA 
GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC 
TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG 
GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA 
AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG 
TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT 
GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA 
CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG 
CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT 
TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT 
GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA 
GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC 
TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT 
ACCAAGGTGA CGGATGAAGG CAAGCAAACT GTTCCCCAAG ACCCCGAAGG CCCTTCAGTT 
CCCAGCAGTT CACCTGTGGC TGAGCAGTCG GTAGTAGTTC CTATGGCTGC AAAAGGGCCG 
CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC 
ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA 
CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA 
CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT 
AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC 
CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT 
GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT 
TTAAAACAGA GCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005640892.1
CDS	444..6341
Translation	MEFGLFIFKLVLFENRFHSWKSLYPGNTPCTGSEEASDPCEAIPRVLMYRGEALEDFIGPDCRFVSFKKGDAVYVYYKLAGRPPEVWAGSVGRIFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFDNYNVEELLGFLELHNSATEESEKAIEETQYEEDTPPEVLEESDLELEPVEVNSEESESVASENTKELKERSGAQKNHPHVNSPADHAQGDPSSFEPFEEMLQDKLKVPESENNKTSNGSQVSNEQQTIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDAEYHIVGKDEEENEDNFDELPLLTFAHGGEDVKTPVKSGVEKYSAESEQSSNEEDKVEAPQPPGIKNYDKDILTTWEDTVFFTEDDSVTSMDLGSSESQEGKEGDDAFIPESKWRKPRSAADYIDPKKAEDGLLPTANDVGTKTDNDEDLEIHIAGKGRGAKEPKPGLAQAEMGPEEEKQEGMTTHGSTHSGDLGSLPSTGKGKEMLESAFDNKEDDLKGAAIHISKGMLHEEKPGEQISEGGSEREPLPQATGGQVNEEKPDQESVGIAAPSGGGQHNASRDRVEDVHALAGLKPHTPSVEHPKEEFREERLLKTQNQPRPSPLDGVGLPGELKEEGPIWGRNLSWPQGGEVAAAHSKQMDEKGRLAEEEVREDSSAEELAPQQPKAGSQEVEPTGRPGSPEVPGVHTEKPEAEDDHSPEELLEDENALSAKQAKEKSPGIQGRWLDVNLQSPSRAPLGVVNTDAEMEGNKEEASTILEIERKGETFGKEVEPGGRGREAGSGVVETESPLAGAKAQTASGGSDSPDKKNRTPGLGEMFQNQDSDYFKEDSAEKSAQKPGVEELSEEDQDFERPADTQSQGPAPEEQEGGPLRGTPPTAVRPMRSDYVKDLPIIGSFFKEQQSLQRFQKYFDVHELEAMFHEMSLKLKLAQQESLPYNVEKVLDKVFRASESQILSTAEKMLDTRVAENRDLGMMESNIFEEAAVLDDIQDLIYFVRYRHSTMEETAPLATGQPPQEGWGGTLEGMQPHLEDNFPQGHTKDNFPQDHIEDSFPQDHTEDSFPQDHTEDNFPQDHTEDLQIQILKEPSQLDQPVVGDMGASEVSQQPNTEKDGDPGIITTESSPVDADDAKKQLQTNAEEPASVTPLENAILLIYSFIFYLTKTLVATLPDDVQPGPDFYGLPWKPVLLTAFLGIGSFAVFFWKTVLVVKSRVYQVTEQQISEKLKNIMKENAELVQKLSNYEQKIKESKKHVQETKKQNIILSDEAIKFKDKIKKLEETNEILDDTAKNLRVMLESEREQNAKNQDLILENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIKSFETSQKNLEVALTHKDDNINALTNCITQLNRLDCESESEDQSKGGSELDELANGEVGGDRSEKMKNQIKQMMDISRTQTAISVVEEDLKLLQIKLRASMSTKCNLEDQIKKLEDDRDSLQSAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKALLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPRPGRPSAQNPPWRGPLSQNGSFGPSPVSGGECSPPPTADLPARPLSATLNRREMPRGEFGSVDGPLPRPRWSSEASGKPSASDLGSGAAPTMNSSSRSSSPTKVTDEGKQTVPQDPEGPSVPSSSPVAEQSVVVPMAAKGPPPFPGVPLMGSPVGGPLPPPIRYGPPPQLCGPFGPRPLPPPFGPALRPPLGLREYAPGIPPGKRDLSLDPREFLPGHAPFRPLGSFGPREYFIPGTRLPPPAHGPQDYPPPPAARDLLPSGSRDEPPPASQGSSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_005640835.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005640835.2

ORF Insert Sequence:

ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT 
GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA 
GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA 
GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG 
GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG 
ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA 
CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA 
GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA 
GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA 
CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT 
GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA 
GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG 
ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT 
GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA 
GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA 
GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT 
GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA 
AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT 
ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG 
GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG 
AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT 
GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA 
ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC 
GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT 
GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT 
GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT 
GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA 
GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA 
CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA 
GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA 
CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA 
GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA 
GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG 
GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG 
GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA 
CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC 
GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG 
CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC 
AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG 
GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT 
CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT 
CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC 
AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC 
TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC 
CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC 
TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC 
TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG 
CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT 
GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA 
GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA 
GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC 
ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT 
GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC 
AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC 
CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT 
CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA 
GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA 
AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT 
CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT 
GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC 
TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT 
AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA 
AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA 
CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG 
GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG 
CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA 
AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA 
GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC 
CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA 
ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA 
TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT 
AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA 
GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA 
ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA 
GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC 
AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA 
GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC 
TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG 
GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA 
AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG 
TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT 
GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA 
CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG 
CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT 
TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT 
GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA 
GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC 
TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT 
ACCAAGGTGA CGGATGAAGG CAAGCAAACT GTTCCCCAAG ACCCCGAAGG CCCTTCAGTT 
CCCAGCAGTT CACCTGTGGC TGAGCAGTCG GTAGTAGTTC CTATGGCTGC AAAAGGGCCG 
CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC 
ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA 
CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA 
CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT 
AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC 
CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT 
GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT 
TTAAAACAGA GCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_005640835.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005640892.1 Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X1, mRNA.

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CloneID	OCe31714
Clone ID Related Accession (Same CDS sequence)	XM_005640835.2 , XM_005640835.3
Accession Version	XM_005640835.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5898bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transport and Golgi organization protein 1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640835.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT 
GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA 
GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA 
GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG 
GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG 
ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA 
CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA 
GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA 
GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA 
CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT 
GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA 
GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG 
ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT 
GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA 
GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA 
GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT 
GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA 
AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT 
ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG 
GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG 
AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT 
GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA 
ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC 
GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT 
GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT 
GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT 
GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA 
GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA 
CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA 
GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA 
CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA 
GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA 
GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG 
GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG 
GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA 
CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC 
GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG 
CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC 
AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG 
GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT 
CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT 
CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC 
AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC 
TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC 
CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC 
TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC 
TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG 
CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT 
GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA 
GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA 
GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC 
ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT 
GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC 
AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC 
CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT 
CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA 
GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA 
AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT 
CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT 
GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC 
TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT 
AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA 
AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA 
CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG 
GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG 
CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA 
AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA 
GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC 
CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA 
ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA 
TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT 
AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA 
GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA 
ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA 
GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC 
AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA 
GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC 
TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG 
GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA 
AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG 
TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT 
GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA 
CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG 
CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT 
TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT 
GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA 
GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC 
TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT 
ACCAAGGTGA CGGATGAAGG CAAGCAAACT GTTCCCCAAG ACCCCGAAGG CCCTTCAGTT 
CCCAGCAGTT CACCTGTGGC TGAGCAGTCG GTAGTAGTTC CTATGGCTGC AAAAGGGCCG 
CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC 
ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA 
CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA 
CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT 
AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC 
CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT 
GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT 
TTAAAACAGA GCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005640892.1
CDS	109..6006
Translation	MEFGLFIFKLVLFENRFHSWKSLYPGNTPCTGSEEASDPCEAIPRVLMYRGEALEDFIGPDCRFVSFKKGDAVYVYYKLAGRPPEVWAGSVGRIFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFDNYNVEELLGFLELHNSATEESEKAIEETQYEEDTPPEVLEESDLELEPVEVNSEESESVASENTKELKERSGAQKNHPHVNSPADHAQGDPSSFEPFEEMLQDKLKVPESENNKTSNGSQVSNEQQTIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDAEYHIVGKDEEENEDNFDELPLLTFAHGGEDVKTPVKSGVEKYSAESEQSSNEEDKVEAPQPPGIKNYDKDILTTWEDTVFFTEDDSVTSMDLGSSESQEGKEGDDAFIPESKWRKPRSAADYIDPKKAEDGLLPTANDVGTKTDNDEDLEIHIAGKGRGAKEPKPGLAQAEMGPEEEKQEGMTTHGSTHSGDLGSLPSTGKGKEMLESAFDNKEDDLKGAAIHISKGMLHEEKPGEQISEGGSEREPLPQATGGQVNEEKPDQESVGIAAPSGGGQHNASRDRVEDVHALAGLKPHTPSVEHPKEEFREERLLKTQNQPRPSPLDGVGLPGELKEEGPIWGRNLSWPQGGEVAAAHSKQMDEKGRLAEEEVREDSSAEELAPQQPKAGSQEVEPTGRPGSPEVPGVHTEKPEAEDDHSPEELLEDENALSAKQAKEKSPGIQGRWLDVNLQSPSRAPLGVVNTDAEMEGNKEEASTILEIERKGETFGKEVEPGGRGREAGSGVVETESPLAGAKAQTASGGSDSPDKKNRTPGLGEMFQNQDSDYFKEDSAEKSAQKPGVEELSEEDQDFERPADTQSQGPAPEEQEGGPLRGTPPTAVRPMRSDYVKDLPIIGSFFKEQQSLQRFQKYFDVHELEAMFHEMSLKLKLAQQESLPYNVEKVLDKVFRASESQILSTAEKMLDTRVAENRDLGMMESNIFEEAAVLDDIQDLIYFVRYRHSTMEETAPLATGQPPQEGWGGTLEGMQPHLEDNFPQGHTKDNFPQDHIEDSFPQDHTEDSFPQDHTEDNFPQDHTEDLQIQILKEPSQLDQPVVGDMGASEVSQQPNTEKDGDPGIITTESSPVDADDAKKQLQTNAEEPASVTPLENAILLIYSFIFYLTKTLVATLPDDVQPGPDFYGLPWKPVLLTAFLGIGSFAVFFWKTVLVVKSRVYQVTEQQISEKLKNIMKENAELVQKLSNYEQKIKESKKHVQETKKQNIILSDEAIKFKDKIKKLEETNEILDDTAKNLRVMLESEREQNAKNQDLILENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIKSFETSQKNLEVALTHKDDNINALTNCITQLNRLDCESESEDQSKGGSELDELANGEVGGDRSEKMKNQIKQMMDISRTQTAISVVEEDLKLLQIKLRASMSTKCNLEDQIKKLEDDRDSLQSAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKALLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPRPGRPSAQNPPWRGPLSQNGSFGPSPVSGGECSPPPTADLPARPLSATLNRREMPRGEFGSVDGPLPRPRWSSEASGKPSASDLGSGAAPTMNSSSRSSSPTKVTDEGKQTVPQDPEGPSVPSSSPVAEQSVVVPMAAKGPPPFPGVPLMGSPVGGPLPPPIRYGPPPQLCGPFGPRPLPPPFGPALRPPLGLREYAPGIPPGKRDLSLDPREFLPGHAPFRPLGSFGPREYFIPGTRLPPPAHGPQDYPPPPAARDLLPSGSRDEPPPASQGSSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_005640835.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005640835.3

ORF Insert Sequence:

ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT 
GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA 
GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA 
GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG 
GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG 
ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA 
CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA 
GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA 
GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA 
CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT 
GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA 
GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG 
ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT 
GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA 
GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA 
GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT 
GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA 
AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT 
ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG 
GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG 
AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT 
GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA 
ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC 
GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT 
GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT 
GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT 
GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA 
GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA 
CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA 
GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA 
CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA 
GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA 
GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG 
GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG 
GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA 
CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC 
GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG 
CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC 
AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG 
GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT 
CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT 
CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC 
AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC 
TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC 
CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC 
TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC 
TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG 
CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT 
GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA 
GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA 
GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC 
ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT 
GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC 
AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC 
CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT 
CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA 
GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA 
AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT 
CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT 
GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC 
TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT 
AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA 
AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA 
CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG 
GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG 
CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA 
AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA 
GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC 
CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA 
ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA 
TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT 
AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA 
GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA 
ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA 
GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC 
AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA 
GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC 
TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG 
GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA 
AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG 
TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT 
GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA 
CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG 
CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT 
TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT 
GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA 
GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC 
TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT 
ACCAAGGTGA CGGATGAAGG CAAGCAAACT GTTCCCCAAG ACCCCGAAGG CCCTTCAGTT 
CCCAGCAGTT CACCTGTGGC TGAGCAGTCG GTAGTAGTTC CTATGGCTGC AAAAGGGCCG 
CCTCCTTTCC CAGGGGTGCC CCTCATGGGC TCCCCCGTGG GCGGCCCTCT ACCACCACCC 
ATTCGCTACG GACCACCCCC TCAACTTTGC GGGCCTTTCG GGCCTCGGCC GCTTCCTCCA 
CCATTTGGTC CTGCTTTACG TCCACCACTA GGCTTAAGAG AATACGCACC AGGCATTCCA 
CCTGGAAAAC GGGACCTGTC TCTTGATCCT CGAGAATTTT TACCGGGACA TGCACCTTTT 
AGACCTTTAG GCTCTTTTGG CCCAAGAGAG TACTTTATTC CCGGTACCCG ACTACCACCC 
CCTGCCCATG GTCCCCAGGA TTATCCACCT CCACCTGCTG CAAGAGACTT ACTGCCTTCT 
GGCTCCAGAG ACGAGCCTCC ACCTGCCTCC CAGGGCAGTA GCCAGGACTG TTCACAGGCT 
TTAAAACAGA GCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_005640836.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005640893.1 Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X2, mRNA.

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CloneID	OCe31715
Clone ID Related Accession (Same CDS sequence)	XM_005640836.2 , XM_005640836.3
Accession Version	XM_005640836.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	melanoma inhibitory activity protein 3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640836.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT 
GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA 
GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA 
GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG 
GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG 
ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA 
CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA 
GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA 
GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA 
CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT 
GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA 
GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG 
ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT 
GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA 
GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA 
GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT 
GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA 
AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT 
ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG 
GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG 
AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT 
GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA 
ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC 
GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT 
GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT 
GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT 
GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA 
GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA 
CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA 
GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA 
CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA 
GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA 
GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG 
GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG 
GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA 
CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC 
GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG 
CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC 
AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG 
GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT 
CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT 
CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC 
AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC 
TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC 
CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC 
TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC 
TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG 
CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT 
GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA 
GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA 
GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC 
ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT 
GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC 
AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC 
CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT 
CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA 
GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA 
AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT 
CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT 
GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC 
TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT 
AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA 
AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA 
CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG 
GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG 
CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA 
AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA 
GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC 
CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA 
ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA 
TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT 
AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA 
GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA 
ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA 
GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC 
AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA 
GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC 
TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG 
GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA 
AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG 
TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT 
GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA 
CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG 
CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT 
TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT 
GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGATCTG 
GGATCTGGTG CAGCTCCCAC GATGAACAGC AGCTCGAGAA GCTCTTCCCC TACCAAGGTG 
ACGGATGAAG GCAAGCAAAC TGTTCCCCAA GACCCCGAAG GCCCTTCAGT TCCCAGCAGT 
TCACCTGTGG CTGAGCAGTC GGTAGTAGTT CCTATGGCTG CAAAAGGGCC GCCTCCTTTC 
CCAGGGGTGC CCCTCATGGG CTCCCCCGTG GGCGGCCCTC TACCACCACC CATTCGCTAC 
GGACCACCCC CTCAACTTTG CGGGCCTTTC GGGCCTCGGC CGCTTCCTCC ACCATTTGGT 
CCTGCTTTAC GTCCACCACT AGGCTTAAGA GAATACGCAC CAGGCATTCC ACCTGGAAAA 
CGGGACCTGT CTCTTGATCC TCGAGAATTT TTACCGGGAC ATGCACCTTT TAGACCTTTA 
GGCTCTTTTG GCCCAAGAGA GTACTTTATT CCCGGTACCC GACTACCACC CCCTGCCCAT 
GGTCCCCAGG ATTATCCACC TCCACCTGCT GCAAGAGACT TACTGCCTTC TGGCTCCAGA 
GACGAGCCTC CACCTGCCTC CCAGGGCAGT AGCCAGGACT GTTCACAGGC TTTAAAACAG 
AGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005640893.1
CDS	545..6373
Translation	MEFGLFIFKLVLFENRFHSWKSLYPGNTPCTGSEEASDPCEAIPRVLMYRGEALEDFIGPDCRFVSFKKGDAVYVYYKLAGRPPEVWAGSVGRIFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFDNYNVEELLGFLELHNSATEESEKAIEETQYEEDTPPEVLEESDLELEPVEVNSEESESVASENTKELKERSGAQKNHPHVNSPADHAQGDPSSFEPFEEMLQDKLKVPESENNKTSNGSQVSNEQQTIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDAEYHIVGKDEEENEDNFDELPLLTFAHGGEDVKTPVKSGVEKYSAESEQSSNEEDKVEAPQPPGIKNYDKDILTTWEDTVFFTEDDSVTSMDLGSSESQEGKEGDDAFIPESKWRKPRSAADYIDPKKAEDGLLPTANDVGTKTDNDEDLEIHIAGKGRGAKEPKPGLAQAEMGPEEEKQEGMTTHGSTHSGDLGSLPSTGKGKEMLESAFDNKEDDLKGAAIHISKGMLHEEKPGEQISEGGSEREPLPQATGGQVNEEKPDQESVGIAAPSGGGQHNASRDRVEDVHALAGLKPHTPSVEHPKEEFREERLLKTQNQPRPSPLDGVGLPGELKEEGPIWGRNLSWPQGGEVAAAHSKQMDEKGRLAEEEVREDSSAEELAPQQPKAGSQEVEPTGRPGSPEVPGVHTEKPEAEDDHSPEELLEDENALSAKQAKEKSPGIQGRWLDVNLQSPSRAPLGVVNTDAEMEGNKEEASTILEIERKGETFGKEVEPGGRGREAGSGVVETESPLAGAKAQTASGGSDSPDKKNRTPGLGEMFQNQDSDYFKEDSAEKSAQKPGVEELSEEDQDFERPADTQSQGPAPEEQEGGPLRGTPPTAVRPMRSDYVKDLPIIGSFFKEQQSLQRFQKYFDVHELEAMFHEMSLKLKLAQQESLPYNVEKVLDKVFRASESQILSTAEKMLDTRVAENRDLGMMESNIFEEAAVLDDIQDLIYFVRYRHSTMEETAPLATGQPPQEGWGGTLEGMQPHLEDNFPQGHTKDNFPQDHIEDSFPQDHTEDSFPQDHTEDNFPQDHTEDLQIQILKEPSQLDQPVVGDMGASEVSQQPNTEKDGDPGIITTESSPVDADDAKKQLQTNAEEPASVTPLENAILLIYSFIFYLTKTLVATLPDDVQPGPDFYGLPWKPVLLTAFLGIGSFAVFFWKTVLVVKSRVYQVTEQQISEKLKNIMKENAELVQKLSNYEQKIKESKKHVQETKKQNIILSDEAIKFKDKIKKLEETNEILDDTAKNLRVMLESEREQNAKNQDLILENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIKSFETSQKNLEVALTHKDDNINALTNCITQLNRLDCESESEDQSKGGSELDELANGEVGGDRSEKMKNQIKQMMDISRTQTAISVVEEDLKLLQIKLRASMSTKCNLEDQIKKLEDDRDSLQSAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKALLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPRPGRPSAQNPPWRGPLSQNGSFGPSPVSGGECSPPPTADLPARPLSATLNRREMPRGEFDLGSGAAPTMNSSSRSSSPTKVTDEGKQTVPQDPEGPSVPSSSPVAEQSVVVPMAAKGPPPFPGVPLMGSPVGGPLPPPIRYGPPPQLCGPFGPRPLPPPFGPALRPPLGLREYAPGIPPGKRDLSLDPREFLPGHAPFRPLGSFGPREYFIPGTRLPPPAHGPQDYPPPPAARDLLPSGSRDEPPPASQGSSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_005640836.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005640836.2

ORF Insert Sequence:

ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT 
GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA 
GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA 
GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG 
GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG 
ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA 
CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA 
GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA 
GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA 
CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT 
GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA 
GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG 
ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT 
GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA 
GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA 
GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT 
GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA 
AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT 
ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG 
GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG 
AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT 
GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA 
ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC 
GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT 
GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT 
GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT 
GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA 
GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA 
CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA 
GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA 
CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA 
GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA 
GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG 
GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG 
GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA 
CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC 
GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG 
CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC 
AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG 
GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT 
CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT 
CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC 
AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC 
TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC 
CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC 
TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC 
TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG 
CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT 
GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA 
GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA 
GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC 
ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT 
GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC 
AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC 
CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT 
CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA 
GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA 
AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT 
CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT 
GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC 
TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT 
AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA 
AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA 
CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG 
GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG 
CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA 
AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA 
GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC 
CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA 
ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA 
TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT 
AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA 
GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA 
ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA 
GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC 
AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA 
GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC 
TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG 
GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA 
AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG 
TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT 
GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA 
CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG 
CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT 
TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT 
GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGATCTG 
GGATCTGGTG CAGCTCCCAC GATGAACAGC AGCTCGAGAA GCTCTTCCCC TACCAAGGTG 
ACGGATGAAG GCAAGCAAAC TGTTCCCCAA GACCCCGAAG GCCCTTCAGT TCCCAGCAGT 
TCACCTGTGG CTGAGCAGTC GGTAGTAGTT CCTATGGCTG CAAAAGGGCC GCCTCCTTTC 
CCAGGGGTGC CCCTCATGGG CTCCCCCGTG GGCGGCCCTC TACCACCACC CATTCGCTAC 
GGACCACCCC CTCAACTTTG CGGGCCTTTC GGGCCTCGGC CGCTTCCTCC ACCATTTGGT 
CCTGCTTTAC GTCCACCACT AGGCTTAAGA GAATACGCAC CAGGCATTCC ACCTGGAAAA 
CGGGACCTGT CTCTTGATCC TCGAGAATTT TTACCGGGAC ATGCACCTTT TAGACCTTTA 
GGCTCTTTTG GCCCAAGAGA GTACTTTATT CCCGGTACCC GACTACCACC CCCTGCCCAT 
GGTCCCCAGG ATTATCCACC TCCACCTGCT GCAAGAGACT TACTGCCTTC TGGCTCCAGA 
GACGAGCCTC CACCTGCCTC CCAGGGCAGT AGCCAGGACT GTTCACAGGC TTTAAAACAG 
AGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_005640836.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005640893.1 Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X2, mRNA.

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CloneID	OCe31715
Clone ID Related Accession (Same CDS sequence)	XM_005640836.2 , XM_005640836.3
Accession Version	XM_005640836.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transport and Golgi organization protein 1 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640836.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT 
GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA 
GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA 
GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG 
GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG 
ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA 
CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA 
GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA 
GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA 
CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT 
GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA 
GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG 
ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT 
GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA 
GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA 
GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT 
GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA 
AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT 
ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG 
GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG 
AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT 
GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA 
ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC 
GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT 
GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT 
GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT 
GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA 
GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA 
CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA 
GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA 
CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA 
GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA 
GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG 
GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG 
GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA 
CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC 
GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG 
CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC 
AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG 
GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT 
CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT 
CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC 
AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC 
TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC 
CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC 
TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC 
TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG 
CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT 
GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA 
GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA 
GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC 
ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT 
GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC 
AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC 
CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT 
CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA 
GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA 
AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT 
CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT 
GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC 
TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT 
AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA 
AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA 
CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG 
GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG 
CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA 
AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA 
GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC 
CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA 
ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA 
TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT 
AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA 
GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA 
ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA 
GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC 
AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA 
GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC 
TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG 
GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA 
AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG 
TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT 
GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA 
CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG 
CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT 
TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT 
GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGATCTG 
GGATCTGGTG CAGCTCCCAC GATGAACAGC AGCTCGAGAA GCTCTTCCCC TACCAAGGTG 
ACGGATGAAG GCAAGCAAAC TGTTCCCCAA GACCCCGAAG GCCCTTCAGT TCCCAGCAGT 
TCACCTGTGG CTGAGCAGTC GGTAGTAGTT CCTATGGCTG CAAAAGGGCC GCCTCCTTTC 
CCAGGGGTGC CCCTCATGGG CTCCCCCGTG GGCGGCCCTC TACCACCACC CATTCGCTAC 
GGACCACCCC CTCAACTTTG CGGGCCTTTC GGGCCTCGGC CGCTTCCTCC ACCATTTGGT 
CCTGCTTTAC GTCCACCACT AGGCTTAAGA GAATACGCAC CAGGCATTCC ACCTGGAAAA 
CGGGACCTGT CTCTTGATCC TCGAGAATTT TTACCGGGAC ATGCACCTTT TAGACCTTTA 
GGCTCTTTTG GCCCAAGAGA GTACTTTATT CCCGGTACCC GACTACCACC CCCTGCCCAT 
GGTCCCCAGG ATTATCCACC TCCACCTGCT GCAAGAGACT TACTGCCTTC TGGCTCCAGA 
GACGAGCCTC CACCTGCCTC CCAGGGCAGT AGCCAGGACT GTTCACAGGC TTTAAAACAG 
AGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005640893.1
CDS	109..5937
Translation	MEFGLFIFKLVLFENRFHSWKSLYPGNTPCTGSEEASDPCEAIPRVLMYRGEALEDFIGPDCRFVSFKKGDAVYVYYKLAGRPPEVWAGSVGRIFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFDNYNVEELLGFLELHNSATEESEKAIEETQYEEDTPPEVLEESDLELEPVEVNSEESESVASENTKELKERSGAQKNHPHVNSPADHAQGDPSSFEPFEEMLQDKLKVPESENNKTSNGSQVSNEQQTIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDAEYHIVGKDEEENEDNFDELPLLTFAHGGEDVKTPVKSGVEKYSAESEQSSNEEDKVEAPQPPGIKNYDKDILTTWEDTVFFTEDDSVTSMDLGSSESQEGKEGDDAFIPESKWRKPRSAADYIDPKKAEDGLLPTANDVGTKTDNDEDLEIHIAGKGRGAKEPKPGLAQAEMGPEEEKQEGMTTHGSTHSGDLGSLPSTGKGKEMLESAFDNKEDDLKGAAIHISKGMLHEEKPGEQISEGGSEREPLPQATGGQVNEEKPDQESVGIAAPSGGGQHNASRDRVEDVHALAGLKPHTPSVEHPKEEFREERLLKTQNQPRPSPLDGVGLPGELKEEGPIWGRNLSWPQGGEVAAAHSKQMDEKGRLAEEEVREDSSAEELAPQQPKAGSQEVEPTGRPGSPEVPGVHTEKPEAEDDHSPEELLEDENALSAKQAKEKSPGIQGRWLDVNLQSPSRAPLGVVNTDAEMEGNKEEASTILEIERKGETFGKEVEPGGRGREAGSGVVETESPLAGAKAQTASGGSDSPDKKNRTPGLGEMFQNQDSDYFKEDSAEKSAQKPGVEELSEEDQDFERPADTQSQGPAPEEQEGGPLRGTPPTAVRPMRSDYVKDLPIIGSFFKEQQSLQRFQKYFDVHELEAMFHEMSLKLKLAQQESLPYNVEKVLDKVFRASESQILSTAEKMLDTRVAENRDLGMMESNIFEEAAVLDDIQDLIYFVRYRHSTMEETAPLATGQPPQEGWGGTLEGMQPHLEDNFPQGHTKDNFPQDHIEDSFPQDHTEDSFPQDHTEDNFPQDHTEDLQIQILKEPSQLDQPVVGDMGASEVSQQPNTEKDGDPGIITTESSPVDADDAKKQLQTNAEEPASVTPLENAILLIYSFIFYLTKTLVATLPDDVQPGPDFYGLPWKPVLLTAFLGIGSFAVFFWKTVLVVKSRVYQVTEQQISEKLKNIMKENAELVQKLSNYEQKIKESKKHVQETKKQNIILSDEAIKFKDKIKKLEETNEILDDTAKNLRVMLESEREQNAKNQDLILENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIKSFETSQKNLEVALTHKDDNINALTNCITQLNRLDCESESEDQSKGGSELDELANGEVGGDRSEKMKNQIKQMMDISRTQTAISVVEEDLKLLQIKLRASMSTKCNLEDQIKKLEDDRDSLQSAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKALLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPRPGRPSAQNPPWRGPLSQNGSFGPSPVSGGECSPPPTADLPARPLSATLNRREMPRGEFDLGSGAAPTMNSSSRSSSPTKVTDEGKQTVPQDPEGPSVPSSSPVAEQSVVVPMAAKGPPPFPGVPLMGSPVGGPLPPPIRYGPPPQLCGPFGPRPLPPPFGPALRPPLGLREYAPGIPPGKRDLSLDPREFLPGHAPFRPLGSFGPREYFIPGTRLPPPAHGPQDYPPPPAARDLLPSGSRDEPPPASQGSSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_005640836.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005640836.3

ORF Insert Sequence:

ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT 
GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA 
GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA 
GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG 
GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG 
ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA 
CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA 
GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA 
GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA 
CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT 
GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA 
GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG 
ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT 
GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA 
GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA 
GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT 
GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA 
AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT 
ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG 
GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG 
AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT 
GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA 
ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC 
GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT 
GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT 
GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT 
GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA 
GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA 
CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA 
GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA 
CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA 
GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA 
GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG 
GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG 
GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA 
CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC 
GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG 
CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC 
AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG 
GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT 
CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT 
CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC 
AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC 
TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC 
CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC 
TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC 
TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG 
CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT 
GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA 
GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA 
GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC 
ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT 
GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC 
AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC 
CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT 
CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA 
GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA 
AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT 
CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT 
GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC 
TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT 
AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA 
AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA 
CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG 
GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG 
CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA 
AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA 
GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC 
CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA 
ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA 
TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT 
AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA 
GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA 
ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA 
GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC 
AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA 
GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC 
TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG 
GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA 
AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG 
TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT 
GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA 
CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG 
CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT 
TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT 
GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGATCTG 
GGATCTGGTG CAGCTCCCAC GATGAACAGC AGCTCGAGAA GCTCTTCCCC TACCAAGGTG 
ACGGATGAAG GCAAGCAAAC TGTTCCCCAA GACCCCGAAG GCCCTTCAGT TCCCAGCAGT 
TCACCTGTGG CTGAGCAGTC GGTAGTAGTT CCTATGGCTG CAAAAGGGCC GCCTCCTTTC 
CCAGGGGTGC CCCTCATGGG CTCCCCCGTG GGCGGCCCTC TACCACCACC CATTCGCTAC 
GGACCACCCC CTCAACTTTG CGGGCCTTTC GGGCCTCGGC CGCTTCCTCC ACCATTTGGT 
CCTGCTTTAC GTCCACCACT AGGCTTAAGA GAATACGCAC CAGGCATTCC ACCTGGAAAA 
CGGGACCTGT CTCTTGATCC TCGAGAATTT TTACCGGGAC ATGCACCTTT TAGACCTTTA 
GGCTCTTTTG GCCCAAGAGA GTACTTTATT CCCGGTACCC GACTACCACC CCCTGCCCAT 
GGTCCCCAGG ATTATCCACC TCCACCTGCT GCAAGAGACT TACTGCCTTC TGGCTCCAGA 
GACGAGCCTC CACCTGCCTC CCAGGGCAGT AGCCAGGACT GTTCACAGGC TTTAAAACAG 
AGCCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_005640837.2 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005640894.1 Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X3, mRNA.

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CloneID	OCe31716
Clone ID Related Accession (Same CDS sequence)	XM_005640837.2 , XM_005640837.3
Accession Version	XM_005640837.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	melanoma inhibitory activity protein 3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005640837.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT 
GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA 
GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA 
GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG 
GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG 
ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA 
CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA 
GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA 
GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA 
CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT 
GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA 
GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG 
ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT 
GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA 
GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA 
GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT 
GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA 
AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT 
ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG 
GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG 
AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT 
GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA 
ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC 
GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT 
GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT 
GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT 
GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA 
GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA 
CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA 
GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA 
CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA 
GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA 
GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG 
GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG 
GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA 
CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC 
GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG 
CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC 
AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG 
GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT 
CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT 
CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC 
AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC 
TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC 
CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC 
TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC 
TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG 
CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT 
GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA 
GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA 
GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC 
ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT 
GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC 
AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC 
CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT 
CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA 
GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA 
AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT 
CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT 
GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC 
TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT 
AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA 
AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA 
CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG 
GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG 
CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA 
AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA 
GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC 
CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA 
ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA 
TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT 
AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA 
GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA 
ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA 
GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC 
AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA 
GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC 
TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG 
GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA 
AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG 
TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT 
GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA 
CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG 
CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT 
TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT 
GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA 
GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC 
TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT 
ACCAAGGTGA CGGATGAAGG CAAGGTTCCT ATGGCTGCAA AAGGGCCGCC TCCTTTCCCA 
GGGGTGCCCC TCATGGGCTC CCCCGTGGGC GGCCCTCTAC CACCACCCAT TCGCTACGGA 
CCACCCCCTC AACTTTGCGG GCCTTTCGGG CCTCGGCCGC TTCCTCCACC ATTTGGTCCT 
GCTTTACGTC CACCACTAGG CTTAAGAGAA TACGCACCAG GCATTCCACC TGGAAAACGG 
GACCTGTCTC TTGATCCTCG AGAATTTTTA CCGGGACATG CACCTTTTAG ACCTTTAGGC 
TCTTTTGGCC CAAGAGAGTA CTTTATTCCC GGTACCCGAC TACCACCCCC TGCCCATGGT 
CCCCAGGATT ATCCACCTCC ACCTGCTGCA AGAGACTTAC TGCCTTCTGG CTCCAGAGAC 
GAGCCTCCAC CTGCCTCCCA GGGCAGTAGC CAGGACTGTT CACAGGCTTT AAAACAGAGC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005640894.1
CDS	439..6264
Translation	MEFGLFIFKLVLFENRFHSWKSLYPGNTPCTGSEEASDPCEAIPRVLMYRGEALEDFIGPDCRFVSFKKGDAVYVYYKLAGRPPEVWAGSVGRIFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFDNYNVEELLGFLELHNSATEESEKAIEETQYEEDTPPEVLEESDLELEPVEVNSEESESVASENTKELKERSGAQKNHPHVNSPADHAQGDPSSFEPFEEMLQDKLKVPESENNKTSNGSQVSNEQQTIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDAEYHIVGKDEEENEDNFDELPLLTFAHGGEDVKTPVKSGVEKYSAESEQSSNEEDKVEAPQPPGIKNYDKDILTTWEDTVFFTEDDSVTSMDLGSSESQEGKEGDDAFIPESKWRKPRSAADYIDPKKAEDGLLPTANDVGTKTDNDEDLEIHIAGKGRGAKEPKPGLAQAEMGPEEEKQEGMTTHGSTHSGDLGSLPSTGKGKEMLESAFDNKEDDLKGAAIHISKGMLHEEKPGEQISEGGSEREPLPQATGGQVNEEKPDQESVGIAAPSGGGQHNASRDRVEDVHALAGLKPHTPSVEHPKEEFREERLLKTQNQPRPSPLDGVGLPGELKEEGPIWGRNLSWPQGGEVAAAHSKQMDEKGRLAEEEVREDSSAEELAPQQPKAGSQEVEPTGRPGSPEVPGVHTEKPEAEDDHSPEELLEDENALSAKQAKEKSPGIQGRWLDVNLQSPSRAPLGVVNTDAEMEGNKEEASTILEIERKGETFGKEVEPGGRGREAGSGVVETESPLAGAKAQTASGGSDSPDKKNRTPGLGEMFQNQDSDYFKEDSAEKSAQKPGVEELSEEDQDFERPADTQSQGPAPEEQEGGPLRGTPPTAVRPMRSDYVKDLPIIGSFFKEQQSLQRFQKYFDVHELEAMFHEMSLKLKLAQQESLPYNVEKVLDKVFRASESQILSTAEKMLDTRVAENRDLGMMESNIFEEAAVLDDIQDLIYFVRYRHSTMEETAPLATGQPPQEGWGGTLEGMQPHLEDNFPQGHTKDNFPQDHIEDSFPQDHTEDSFPQDHTEDNFPQDHTEDLQIQILKEPSQLDQPVVGDMGASEVSQQPNTEKDGDPGIITTESSPVDADDAKKQLQTNAEEPASVTPLENAILLIYSFIFYLTKTLVATLPDDVQPGPDFYGLPWKPVLLTAFLGIGSFAVFFWKTVLVVKSRVYQVTEQQISEKLKNIMKENAELVQKLSNYEQKIKESKKHVQETKKQNIILSDEAIKFKDKIKKLEETNEILDDTAKNLRVMLESEREQNAKNQDLILENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIKSFETSQKNLEVALTHKDDNINALTNCITQLNRLDCESESEDQSKGGSELDELANGEVGGDRSEKMKNQIKQMMDISRTQTAISVVEEDLKLLQIKLRASMSTKCNLEDQIKKLEDDRDSLQSAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKALLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPRPGRPSAQNPPWRGPLSQNGSFGPSPVSGGECSPPPTADLPARPLSATLNRREMPRGEFGSVDGPLPRPRWSSEASGKPSASDLGSGAAPTMNSSSRSSSPTKVTDEGKVPMAAKGPPPFPGVPLMGSPVGGPLPPPIRYGPPPQLCGPFGPRPLPPPFGPALRPPLGLREYAPGIPPGKRDLSLDPREFLPGHAPFRPLGSFGPREYFIPGTRLPPPAHGPQDYPPPPAARDLLPSGSRDEPPPASQGSSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_005640837.2
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005640837.2

ORF Insert Sequence:

ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT 
GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA 
GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA 
GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG 
GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG 
ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA 
CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA 
GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA 
GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA 
CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT 
GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA 
GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG 
ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT 
GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA 
GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA 
GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT 
GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA 
AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT 
ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG 
GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG 
AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT 
GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA 
ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC 
GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT 
GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT 
GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT 
GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA 
GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA 
CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA 
GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA 
CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA 
GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA 
GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG 
GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG 
GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA 
CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC 
GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG 
CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC 
AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG 
GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT 
CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT 
CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC 
AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC 
TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC 
CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC 
TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC 
TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG 
CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT 
GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA 
GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA 
GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC 
ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT 
GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC 
AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC 
CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT 
CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA 
GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA 
AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT 
CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT 
GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC 
TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT 
AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA 
AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA 
CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG 
GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG 
CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA 
AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA 
GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC 
CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA 
ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA 
TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT 
AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA 
GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA 
ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA 
GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC 
AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA 
GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC 
TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG 
GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA 
AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG 
TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT 
GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA 
CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG 
CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT 
TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT 
GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA 
GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC 
TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT 
ACCAAGGTGA CGGATGAAGG CAAGGTTCCT ATGGCTGCAA AAGGGCCGCC TCCTTTCCCA 
GGGGTGCCCC TCATGGGCTC CCCCGTGGGC GGCCCTCTAC CACCACCCAT TCGCTACGGA 
CCACCCCCTC AACTTTGCGG GCCTTTCGGG CCTCGGCCGC TTCCTCCACC ATTTGGTCCT 
GCTTTACGTC CACCACTAGG CTTAAGAGAA TACGCACCAG GCATTCCACC TGGAAAACGG 
GACCTGTCTC TTGATCCTCG AGAATTTTTA CCGGGACATG CACCTTTTAG ACCTTTAGGC 
TCTTTTGGCC CAAGAGAGTA CTTTATTCCC GGTACCCGAC TACCACCCCC TGCCCATGGT 
CCCCAGGATT ATCCACCTCC ACCTGCTGCA AGAGACTTAC TGCCTTCTGG CTCCAGAGAC 
GAGCCTCCAC CTGCCTCCCA GGGCAGTAGC CAGGACTGTT CACAGGCTTT AAAACAGAGC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_005640837.3 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_005640894.1 Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X3, mRNA.

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CloneID	OCe31716
Clone ID Related Accession (Same CDS sequence)	XM_005640837.2 , XM_005640837.3
Accession Version	XM_005640837.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	transport and Golgi organization protein 1 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006620.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005640837.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT 
GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA 
GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA 
GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG 
GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG 
ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA 
CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA 
GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA 
GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA 
CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT 
GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA 
GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG 
ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT 
GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA 
GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA 
GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT 
GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA 
AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT 
ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG 
GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG 
AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT 
GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA 
ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC 
GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT 
GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT 
GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT 
GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA 
GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA 
CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA 
GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA 
CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA 
GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA 
GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG 
GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG 
GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA 
CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC 
GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG 
CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC 
AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG 
GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT 
CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT 
CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC 
AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC 
TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC 
CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC 
TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC 
TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG 
CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT 
GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA 
GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA 
GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC 
ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT 
GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC 
AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC 
CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT 
CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA 
GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA 
AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT 
CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT 
GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC 
TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT 
AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA 
AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA 
CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG 
GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG 
CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA 
AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA 
GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC 
CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA 
ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA 
TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT 
AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA 
GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA 
ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA 
GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC 
AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA 
GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC 
TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG 
GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA 
AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG 
TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT 
GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA 
CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG 
CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT 
TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT 
GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA 
GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC 
TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT 
ACCAAGGTGA CGGATGAAGG CAAGGTTCCT ATGGCTGCAA AAGGGCCGCC TCCTTTCCCA 
GGGGTGCCCC TCATGGGCTC CCCCGTGGGC GGCCCTCTAC CACCACCCAT TCGCTACGGA 
CCACCCCCTC AACTTTGCGG GCCTTTCGGG CCTCGGCCGC TTCCTCCACC ATTTGGTCCT 
GCTTTACGTC CACCACTAGG CTTAAGAGAA TACGCACCAG GCATTCCACC TGGAAAACGG 
GACCTGTCTC TTGATCCTCG AGAATTTTTA CCGGGACATG CACCTTTTAG ACCTTTAGGC 
TCTTTTGGCC CAAGAGAGTA CTTTATTCCC GGTACCCGAC TACCACCCCC TGCCCATGGT 
CCCCAGGATT ATCCACCTCC ACCTGCTGCA AGAGACTTAC TGCCTTCTGG CTCCAGAGAC 
GAGCCTCCAC CTGCCTCCCA GGGCAGTAGC CAGGACTGTT CACAGGCTTT AAAACAGAGC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005640894.1
CDS	107..5932
Translation	MEFGLFIFKLVLFENRFHSWKSLYPGNTPCTGSEEASDPCEAIPRVLMYRGEALEDFIGPDCRFVSFKKGDAVYVYYKLAGRPPEVWAGSVGRIFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFDNYNVEELLGFLELHNSATEESEKAIEETQYEEDTPPEVLEESDLELEPVEVNSEESESVASENTKELKERSGAQKNHPHVNSPADHAQGDPSSFEPFEEMLQDKLKVPESENNKTSNGSQVSNEQQTIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDAEYHIVGKDEEENEDNFDELPLLTFAHGGEDVKTPVKSGVEKYSAESEQSSNEEDKVEAPQPPGIKNYDKDILTTWEDTVFFTEDDSVTSMDLGSSESQEGKEGDDAFIPESKWRKPRSAADYIDPKKAEDGLLPTANDVGTKTDNDEDLEIHIAGKGRGAKEPKPGLAQAEMGPEEEKQEGMTTHGSTHSGDLGSLPSTGKGKEMLESAFDNKEDDLKGAAIHISKGMLHEEKPGEQISEGGSEREPLPQATGGQVNEEKPDQESVGIAAPSGGGQHNASRDRVEDVHALAGLKPHTPSVEHPKEEFREERLLKTQNQPRPSPLDGVGLPGELKEEGPIWGRNLSWPQGGEVAAAHSKQMDEKGRLAEEEVREDSSAEELAPQQPKAGSQEVEPTGRPGSPEVPGVHTEKPEAEDDHSPEELLEDENALSAKQAKEKSPGIQGRWLDVNLQSPSRAPLGVVNTDAEMEGNKEEASTILEIERKGETFGKEVEPGGRGREAGSGVVETESPLAGAKAQTASGGSDSPDKKNRTPGLGEMFQNQDSDYFKEDSAEKSAQKPGVEELSEEDQDFERPADTQSQGPAPEEQEGGPLRGTPPTAVRPMRSDYVKDLPIIGSFFKEQQSLQRFQKYFDVHELEAMFHEMSLKLKLAQQESLPYNVEKVLDKVFRASESQILSTAEKMLDTRVAENRDLGMMESNIFEEAAVLDDIQDLIYFVRYRHSTMEETAPLATGQPPQEGWGGTLEGMQPHLEDNFPQGHTKDNFPQDHIEDSFPQDHTEDSFPQDHTEDNFPQDHTEDLQIQILKEPSQLDQPVVGDMGASEVSQQPNTEKDGDPGIITTESSPVDADDAKKQLQTNAEEPASVTPLENAILLIYSFIFYLTKTLVATLPDDVQPGPDFYGLPWKPVLLTAFLGIGSFAVFFWKTVLVVKSRVYQVTEQQISEKLKNIMKENAELVQKLSNYEQKIKESKKHVQETKKQNIILSDEAIKFKDKIKKLEETNEILDDTAKNLRVMLESEREQNAKNQDLILENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIKSFETSQKNLEVALTHKDDNINALTNCITQLNRLDCESESEDQSKGGSELDELANGEVGGDRSEKMKNQIKQMMDISRTQTAISVVEEDLKLLQIKLRASMSTKCNLEDQIKKLEDDRDSLQSAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKALLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPRPGRPSAQNPPWRGPLSQNGSFGPSPVSGGECSPPPTADLPARPLSATLNRREMPRGEFGSVDGPLPRPRWSSEASGKPSASDLGSGAAPTMNSSSRSSSPTKVTDEGKVPMAAKGPPPFPGVPLMGSPVGGPLPPPIRYGPPPQLCGPFGPRPLPPPFGPALRPPLGLREYAPGIPPGKRDLSLDPREFLPGHAPFRPLGSFGPREYFIPGTRLPPPAHGPQDYPPPPAARDLLPSGSRDEPPPASQGSSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_005640837.3
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris MIA family member 3, ER export factor (MIA3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005640837.3

ORF Insert Sequence:

ATGGAGTTTG GCTTATTCAT ATTCAAATTG GTGCTATTCG AGAACAGATT TCATAGTTGG 
AAGAGTTTGT ATCCCGGCAA CACGCCGTGC ACGGGAAGCG AGGAGGCATC TGATCCGTGT 
GAAGCCATTC CTAGAGTGTT AATGTACCGA GGTGAGGCTC TAGAAGATTT TATAGGCCCA 
GATTGTCGTT TCGTGAGTTT TAAAAAAGGT GATGCTGTGT ATGTCTACTA TAAACTGGCA 
GGGAGACCGC CTGAAGTTTG GGCCGGAAGT GTTGGGCGTA TTTTTGGATA TTTTCCAAAG 
GATTTAATAC AAGTAGTCCA TGAATATACT AAAGAGGAGC TACAGGTTCC AACAGATGAG 
ACAGATTTTG TTTGTTTTGA TGGGGGAAGA GATGATTTTG ATAATTATAA TGTAGAAGAA 
CTTTTAGGAT TTTTGGAATT ACACAATTCT GCAACTGAAG AGTCTGAGAA AGCCATAGAA 
GAAACTCAGT ATGAGGAGGA CACCCCTCCT GAAGTATTGG AGGAAAGCGA CCTCGAACTA 
GAACCAGTAG AAGTTAACTC AGAGGAGAGT GAAAGTGTAG CCTCAGAAAA CACTAAGGAA 
CTCAAGGAAA GATCTGGGGC TCAGAAGAAC CACCCGCATG TAAACAGTCC AGCAGATCAT 
GCTCAGGGAG ATCCATCTTC ATTTGAACCT TTTGAGGAAA TGCTACAAGA TAAACTAAAA 
GTGCCAGAAA GTGAAAACAA CAAAACCAGC AATGGTTCTC AGGTCTCGAA TGAACAGCAG 
ACAATTGATG CTTATAAACT TTTGAAAAAA GAAATGACCC TGGACTTAAA AACCAAATTT 
GGCTCCACTG CTGATGCACT TGTATCTGAT GATGAGACAA CCAGACTTGT TACTTCATTA 
GAAGATGATT TTGATGAGGA ATTGGATGCT GAGTATCACA TAGTTGGCAA GGACGAAGAA 
GAGAATGAAG ACAACTTTGA CGAGCTGCCG TTACTGACCT TCGCACATGG TGGAGAAGAT 
GTGAAAACTC CAGTGAAGTC CGGAGTTGAG AAATACTCAG CAGAGAGCGA GCAGAGTTCA 
AATGAAGAGG ATAAGGTTGA GGCACCTCAG CCCCCTGGCA TCAAAAACTA TGATAAAGAT 
ATATTAACAA CCTGGGAGGA TACTGTCTTC TTCACAGAAG ATGACAGCGT AACAAGTATG 
GATTTGGGGA GTTCTGAATC ACAGGAAGGC AAGGAAGGTG ATGATGCGTT CATCCCAGAG 
AGCAAATGGA GGAAACCACG GTCAGCAGCG GATTACATTG ACCCTAAGAA GGCAGAAGAT 
GGTCTTTTGC CTACAGCTAA CGATGTAGGT ACTAAAACAG ATAACGACGA AGACCTGGAA 
ATTCACATTG CAGGAAAAGG GAGGGGAGCC AAGGAACCCA AGCCGGGCCT GGCACAGGCC 
GAGATGGGGC CGGAGGAGGA GAAGCAGGAA GGCATGACCA CACACGGTTC TACTCATAGT 
GGTGACCTCG GCTCTTTGCC ATCTACTGGA AAGGGTAAAG AAATGTTGGA ATCAGCATTT 
GATAACAAAG AAGATGACCT AAAAGGAGCG GCTATTCATA TTTCAAAAGG AATGCTCCAT 
GAAGAAAAGC CCGGAGAGCA GATTTCAGAA GGGGGCTCGG AGCGTGAACC CCTCCCTCAA 
GCCACAGGGG GTCAAGTGAA CGAAGAAAAG CCTGACCAGG AGTCTGTGGG TATCGCAGCA 
CCCTCTGGGG GTGGTCAGCA TAATGCCTCC AGGGACCGTG TGGAAGACGT CCACGCTTTA 
GCAGGATTGA AACCACATAC ACCGTCAGTG GAGCATCCAA AGGAGGAATT TAGAGAGGAA 
CGGCTTCTTA AAACTCAGAA TCAACCTAGG CCCTCCCCTC TGGATGGAGT TGGTTTGCCA 
GGAGAACTGA AAGAAGAGGG TCCCATTTGG GGAAGAAATC TTTCCTGGCC GCAAGGAGGA 
GAAGTGGCTG CTGCACATAG TAAGCAGATG GATGAGAAGG GGAGGCTTGC CGAGGAGGAG 
GTCAGAGAGG ACTCCTCAGC TGAAGAGTTA GCTCCTCAAC AGCCCAAGGC GGGCTCCCAG 
GAAGTGGAGC CCACAGGCCG GCCAGGTAGC CCGGAAGTGC CAGGTGTCCA TACTGAGAAA 
CCAGAGGCAG AAGACGATCA CAGCCCTGAA GAACTCCTGG AGGATGAAAA TGCTCTCAGC 
GCAAAACAGG CTAAAGAAAA AAGCCCCGGG ATTCAGGGCA GGTGGTTAGA CGTTAACCTG 
CAGTCCCCCT CAAGAGCTCC TTTAGGTGTG GTTAATACCG ATGCAGAAAT GGAAGGAAAC 
AAAGAAGAAG CTAGTACTAT TTTGGAAATA GAAAGAAAAG GTGAAACTTT TGGCAAAGAG 
GTAGAGCCAG GGGGCAGGGG CAGGGAAGCA GGTAGTGGGG TGGTGGAAAC AGAAAGCCCT 
CTTGCAGGTG CAAAAGCACA GACGGCATCT GGAGGAAGTG ACTCTCCTGA CAAGAAAAAT 
CGGACTCCGG GGTTAGGTGA AATGTTTCAG AATCAAGACT CTGATTATTT TAAAGAAGAC 
AGCGCTGAGA AATCTGCACA GAAGCCTGGG GTAGAAGAAC TCTCTGAAGA GGACCAGGAC 
TTTGAGAGAC CCGCGGACAC ACAGAGCCAG GGCCCTGCCC CCGAGGAGCA GGAAGGTGGC 
CCGCTGCGCG GGACGCCACC CACGGCGGTG AGGCCAATGC GTAGCGACTA CGTGAAGGAC 
TTGCCCATAA TTGGCAGCTT CTTTAAAGAA CAACAGTCTC TGCAGAGGTT CCAGAAGTAC 
TTCGATGTCC ACGAGCTGGA AGCCATGTTC CACGAAATGT CATTAAAGCT GAAGTTGGCG 
CAGCAGGAGA GCCTGCCTTA TAACGTGGAG AAAGTCCTAG ATAAGGTCTT CCGTGCATCT 
GAGTCACAGA TTCTGAGCAC AGCAGAGAAA ATGCTTGATA CCCGTGTGGC TGAAAACAGA 
GATCTGGGAA TGATGGAAAG TAACATATTT GAGGAGGCAG CCGTACTAGA CGATATTCAA 
GACCTGATCT ATTTTGTCAG GTACAGACAC TCCACCATGG AGGAGACTGC CCCGCTGGCC 
ACGGGACAGC CCCCCCAGGA AGGCTGGGGT GGCACGTTGG AAGGGATGCA GCCACACCTT 
GAAGATAATT TCCCACAAGG TCACACCAAA GATAATTTCC CACAAGACCA CATAGAAGAC 
AGTTTCCCAC AAGATCACAC AGAAGACAGT TTCCCACAAG ATCACACAGA AGACAATTTC 
CCACAAGATC ACACAGAAGA CCTTCAAATA CAGATTCTCA AAGAGCCCAG CCAGTTGGAT 
CAACCTGTGG TTGGTGACAT GGGTGCCTCA GAAGTGTCAC AGCAGCCAAA TACTGAGAAA 
GATGGAGACC CAGGGATAAT TACAACAGAA AGCAGTCCTG TTGATGCTGA TGATGCAAAA 
AAGCAACTAC AAACCAATGC TGAAGAGCCC GCAAGCGTCA CACCTTTGGA AAATGCAATT 
CTTTTAATAT ATTCATTCAT ATTTTATTTA ACTAAGACGT TAGTTGCTAC ATTGCCTGAT 
GACGTTCAGC CTGGGCCTGA TTTTTATGGC TTGCCATGGA AGCCTGTACT TCTCACTGCC 
TTCTTGGGAA TTGGTTCATT TGCGGTCTTC TTCTGGAAAA CTGTCCTTGT TGTAAAGAGT 
AGAGTCTATC AAGTCACTGA ACAACAAATT TCTGAGAAGT TGAAGAACAT CATGAAAGAA 
AATGCAGAAC TTGTACAAAA ATTGTCAAAT TATGAACAGA AGATTAAGGA ATCAAAAAAA 
CATGTTCAAG AAACCAAGAA ACAAAATATA ATTCTGTCTG ATGAAGCAAT TAAATTTAAG 
GATAAAATCA AGAAACTTGA AGAAACAAAT GAAATTCTGG ACGATACAGC TAAAAATCTG 
CGTGTTATGT TAGAATCTGA GAGAGAACAG AATGCCAAGA ATCAGGACTT GATACTGGAA 
AACAAGAAAT CTATAGAGAA GCTGAAGGAT GTTATTTCAG TGAATGCCTC AGAATTTTCA 
GAGGTTCAAA TTGCCCTTAA TGAAGCTAAA CTTAGTGAAG AGAAGGTGAA GTCTGAATGC 
CATCGAGTTC AAGAAGAAAA TGCTAGGCTT AAGAAGAAGA AGGAACAGTT GCAGCAAGAA 
ATCAAAGATT GGAGCAAATC ACACGCTGAG CTCAGTGAGC AAATCAAATC ATTTGAGACA 
TCTCAGAAAA ATTTGGAAGT AGCTCTTACT CACAAAGATG ATAATATTAA TGCTTTGACT 
AATTGCATTA CCCAGTTGAA TCGGTTAGAT TGTGAATCTG AATCTGAGGA TCAGAGTAAA 
GGAGGAAGTG AGTTAGATGA GTTAGCAAAT GGAGAAGTAG GAGGTGACCG GAGTGAGAAA 
ATGAAAAATC AGATTAAGCA GATGATGGAC ATTTCTCGGA CACAAACTGC AATATCAGTA 
GTTGAAGAGG ATCTAAAACT TCTGCAGATT AAGCTTAGAG CCTCAATGTC TACTAAATGC 
AACCTGGAAG ACCAAATAAA GAAATTAGAA GATGACCGTG ATTCACTGCA GTCTGCCAAA 
GCCGGCCTGG AGGACGAATG CAAAACCCTG AGGCAAAAAG TGGAGATTCT GAACGAACTC 
TATCAACAGA AGGAAATGGC TCTGCAAAAG AAACTGAGTC AGGAAGAGTA TGAGCGGCAG 
GAGAGAGAGC AGCGGCTGTC AGCTGCAGAC GAGAAGGCGC TTCTGGCTGC GGAGGAAGTA 
AAAACGTACA AGCGTAGAAT CGAAGAGATG GAGGATGAGT TGCAGAAGAC AGAGCGCTCG 
TTTAAAAACC AGATTGCGAC TCATGAGAAG AAAGCTCATG ATAACTGGCT CAAAGCTCGT 
GCTGCAGAAA GAGCTATAGC CGAGGAGAAG AGGGAAGCTG CCAACTTGAG ACACAAGTTA 
CTGGAATTAA CCCAGAAGAT GGCAATGCTG CAAGAGGAGC CTGTGATTGT GAAGCCGAGG 
CCGGGCCGAC CCAGCGCCCA GAACCCTCCG TGGAGAGGTC CTTTGAGCCA GAACGGCTCT 
TTTGGTCCAT CCCCTGTGAG TGGGGGGGAG TGCTCCCCAC CTCCCACGGC TGACCTGCCT 
GCGAGACCGC TCTCTGCTAC GCTGAATCGA AGAGAGATGC CTAGAGGTGA ATTTGGGTCA 
GTGGACGGGC CTCTACCTCG TCCTCGTTGG TCATCTGAGG CATCTGGGAA ACCCTCTGCC 
TCTGATCTGG GATCTGGTGC AGCTCCCACG ATGAACAGCA GCTCGAGAAG CTCTTCCCCT 
ACCAAGGTGA CGGATGAAGG CAAGGTTCCT ATGGCTGCAA AAGGGCCGCC TCCTTTCCCA 
GGGGTGCCCC TCATGGGCTC CCCCGTGGGC GGCCCTCTAC CACCACCCAT TCGCTACGGA 
CCACCCCCTC AACTTTGCGG GCCTTTCGGG CCTCGGCCGC TTCCTCCACC ATTTGGTCCT 
GCTTTACGTC CACCACTAGG CTTAAGAGAA TACGCACCAG GCATTCCACC TGGAAAACGG 
GACCTGTCTC TTGATCCTCG AGAATTTTTA CCGGGACATG CACCTTTTAG ACCTTTAGGC 
TCTTTTGGCC CAAGAGAGTA CTTTATTCCC GGTACCCGAC TACCACCCCC TGCCCATGGT 
CCCCAGGATT ATCCACCTCC ACCTGCTGCA AGAGACTTAC TGCCTTCTGG CTCCAGAGAC 
GAGCCTCCAC CTGCCTCCCA GGGCAGTAGC CAGGACTGTT CACAGGCTTT AAAACAGAGC 
CCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MIA3 ( XM_014111163.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013966638.1 Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X4, mRNA.

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CloneID	OCe45301
Clone ID Related Accession (Same CDS sequence)	XM_014111163.1
Accession Version	XM_014111163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	melanoma inhibitory activity protein 3 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726124.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCGAG GTGAGGCTCT AGAAGATTTT ATAGGCCCAG ATTGTCGTTT CGTGAGTTTT 
AAAAAAGGTG ATGCTGTGTA TGTCTACTAT AAACTGGCAG GGAGACCGCC TGAAGTTTGG 
GCCGGAAGTG TTGGGCGTAT TTTTGGATAT TTTCCAAAGG ATTTAATACA AGTAGTCCAT 
GAATATACTA AAGAGGAGCT ACAGGTTCCA ACAGATGAGA CAGATTTTGT TTGTTTTGAT 
GGGGGAAGAG ATGATTTTGA TAATTATAAT GTAGAAGAAC TTTTAGGATT TTTGGAATTA 
CACAATTCTG CAACTGAAGA GTCTGAGAAA GCCATAGAAG AAACTCAGTA TGAGGAGGAC 
ACCCCTCCTG AAGTATTGGA GGAAAGCGAC CTCGAACTAG AACCAGTAGA AGTTAACTCA 
GAGGAGAGTG AAAGTGTAGC CTCAGAAAAC ACTAAGGAAC TCAAGGAAAG ATCTGGGGCT 
CAGAAGAACC ACCCGCATGT AAACAGTCCA GCAGATCATG CTCAGGGAGA TCCATCTTCA 
TTTGAACCTT TTGAGGAAAT GCTACAAGAT AAACTAAAAG TGCCAGAAAG TGAAAACAAC 
AAAACCAGCA ATGGTTCTCA GGTCTCGAAT GAACAGCAGA CAATTGATGC TTATAAACTT 
TTGAAAAAAG AAATGACCCT GGACTTAAAA ACCAAATTTG GCTCCACTGC TGATGCACTT 
GTATCTGATG ATGAGACAAC CAGACTTGTT ACTTCATTAG AAGATGATTT TGATGAGGAA 
TTGGATGCTG AGTATCACAT AGTTGGCAAG GACGAAGAAG AGAATGAAGA CAACTTTGAC 
GAGCTGCCGT TACTGACCTT CGCACATGGT GGAGAAGATG TGAAAACTCC AGTGAAGTCC 
GGAGTTGAGA AATACTCAGC AGAGAGCGAG CAGAGTTCAA ATGAAGAGGA TAAGGTTGAG 
GCACCTCAGC CCCCTGGCAT CAAAAACTAT GATAAAGATA TATTAACAAC CTGGGAGGAT 
ACTGTCTTCT TCACAGAAGA TGACAGCGTA ACAAGTATGG ATTTGGGGAG TTCTGAATCA 
CAGGAAGGCA AGGAAGGTGA TGATGCGTTC ATCCCAGAGA GCAAATGGAG GAAACCACGG 
TCAGCAGCGG ATTACATTGA CCCTAAGAAG GCAGAAGATG GTCTTTTGCC TACAGCTAAC 
GATGTAGGTA CTAAAACAGA TAACGACGAA GACCTGGAAA TTCACATTGC AGGAAAAGGG 
AGGGGAGCCA AGGAACCCAA GCCGGGCCTG GCACAGGCCG AGATGGGGCC GGAGGAGGAG 
AAGCAGGAAG GCATGACCAC ACACGGTTCT ACTCATAGTG GTGACCTCGG CTCTTTGCCA 
TCTACTGGAA AGGGTAAAGA AATGTTGGAA TCAGCATTTG ATAACAAAGA AGATGACCTA 
AAAGGAGCGG CTATTCATAT TTCAAAAGGA ATGCTCCATG AAGAAAAGCC CGGAGAGCAG 
ATTTCAGAAG GGGGCTCGGA GCGTGAACCC CTCCCTCAAG CCACAGGGGG TCAAGTGAAC 
GAAGAAAAGC CTGACCAGGA GTCTGTGGGT ATCGCAGCAC CCTCTGGGGG TGGTCAGCAT 
AATGCCTCCA GGGACCGTGT GGAAGACGTC CACGCTTTAG CAGGATTGAA ACCACATACA 
CCGTCAGTGG AGCATCCAAA GGAGGAATTT AGAGAGGAAC GGCTTCTTAA AACTCAGAAT 
CAACCTAGGC CCTCCCCTCT GGATGGAGTT GGTTTGCCAG GAGAACTGAA AGAAGAGGGT 
CCCATTTGGG GAAGAAATCT TTCCTGGCCG CAAGGAGGAG AAGTGGCTGC TGCACATAGT 
AAGCAGATGG ATGAGAAGGG GAGGCTTGCC GAGGAGGAGG TCAGAGAGGA CTCCTCAGCT 
GAAGAGTTAG CTCCTCAACA GCCCAAGGCG GGCTCCCAGG AAGTGGAGCC CACAGGCCGG 
CCAGGTAGCC CGGAAGTGCC AGGTGTCCAT ACTGAGAAAC CAGAGGCAGA AGACGATCAC 
AGCCCTGAAG AACTCCTGGA GGATGAAAAT GCTCTCAGCG CAAAACAGGC TAAAGAAAAA 
AGCCCCGGGA TTCAGGGCAG GTGGTTAGAC GTTAACCTGC AGTCCCCCTC AAGAGCTCCT 
TTAGGTGTGG TTAATACCGA TGCAGAAATG GAAGGAAACA AAGAAGAAGC TAGTACTATT 
TTGGAAATAG AAAGAAAAGG TGAAACTTTT GGCAAAGAGG TAGAGCCAGG GGGCAGGGGC 
AGGGAAGCAG GTAGTGGGGT GGTGGAAACA GAAAGCCCTC TTGCAGGTGC AAAAGCACAG 
ACGGCATCTG GAGGAAGTGA CTCTCCTGAC AAGAAAAATC GGACTCCGGG GTTAGGTGAA 
ATGTTTCAGA ATCAAGACTC TGATTATTTT AAAGAAGACA GCGCTGAGAA ATCTGCACAG 
AAGCCTGGGG TAGAAGAACT CTCTGAAGAG GACCAGGACT TTGAGAGACC CGCGGACACA 
CAGAGCCAGG GCCCTGCCCC CGAGGAGCAG GAAGGTGGCC CGCTGCGCGG GACGCCACCC 
ACGGCGGTGA GGCCAATGCG TAGCGACTAC GTGAAGGACT TGCCCATAAT TGGCAGCTTC 
TTTAAAGAAC AACAGTCTCT GCAGAGGTTC CAGAAGTACT TCGATGTCCA CGAGCTGGAA 
GCCATGTTCC ACGAAATGTC ATTAAAGCTG AAGTTGGCGC AGCAGGAGAG CCTGCCTTAT 
AACGTGGAGA AAGTCCTAGA TAAGGTCTTC CGTGCATCTG AGTCACAGAT TCTGAGCACA 
GCAGAGAAAA TGCTTGATAC CCGTGTGGCT GAAAACAGAG ATCTGGGAAT GATGGAAAGT 
AACATATTTG AGGAGGCAGC CGTACTAGAC GATATTCAAG ACCTGATCTA TTTTGTCAGG 
TACAGACACT CCACCATGGA GGAGACTGCC CCGCTGGCCA CGGGACAGCC CCCCCAGGAA 
GGCTGGGGTG GCACGTTGGA AGGGATGCAG CCACACCTTG AAGATAATTT CCCACAAGGT 
CACACCAAAG ATAATTTCCC ACAAGACCAC ATAGAAGACA GTTTCCCACA AGATCACACA 
GAAGACAGTT TCCCACAAGA TCACACAGAA GACAATTTCC CACAAGATCA CACAGAAGAC 
CTTCAAATAC AGATTCTCAA AGAGCCCAGC CAGTTGGATC AACCTGTGGT TGGTGACATG 
GGTGCCTCAG AAGTGTCACA GCAGCCAAAT ACTGAGAAAG ATGGAGACCC AGGGATAATT 
ACAACAGAAA GCAGTCCTGT TGATGCTGAT GATGCAAAAA AGCAACTACA AACCAATGCT 
GAAGAGCCCG CAAGCGTCAC ACCTTTGGAA AATGCAATTC TTTTAATATA TTCATTCATA 
TTTTATTTAA CTAAGACGTT AGTTGCTACA TTGCCTGATG ACGTTCAGCC TGGGCCTGAT 
TTTTATGGCT TGCCATGGAA GCCTGTACTT CTCACTGCCT TCTTGGGAAT TGGTTCATTT 
GCGGTCTTCT TCTGGAAAAC TGTCCTTGTT GTAAAGAGTA GAGTCTATCA AGTCACTGAA 
CAACAAATTT CTGAGAAGTT GAAGAACATC ATGAAAGAAA ATGCAGAACT TGTACAAAAA 
TTGTCAAATT ATGAACAGAA GATTAAGGAA TCAAAAAAAC ATGTTCAAGA AACCAAGAAA 
CAAAATATAA TTCTGTCTGA TGAAGCAATT AAATTTAAGG ATAAAATCAA GAAACTTGAA 
GAAACAAATG AAATTCTGGA CGATACAGCT AAAAATCTGC GTGTTATGTT AGAATCTGAG 
AGAGAACAGA ATGCCAAGAA TCAGGACTTG ATACTGGAAA ACAAGAAATC TATAGAGAAG 
CTGAAGGATG TTATTTCAGT GAATGCCTCA GAATTTTCAG AGGTTCAAAT TGCCCTTAAT 
GAAGCTAAAC TTAGTGAAGA GAAGGTGAAG TCTGAATGCC ATCGAGTTCA AGAAGAAAAT 
GCTAGGCTTA AGAAGAAGAA GGAACAGTTG CAGCAAGAAA TCAAAGATTG GAGCAAATCA 
CACGCTGAGC TCAGTGAGCA AATCAAATCA TTTGAGACAT CTCAGAAAAA TTTGGAAGTA 
GCTCTTACTC ACAAAGATGA TAATATTAAT GCTTTGACTA ATTGCATTAC CCAGTTGAAT 
CGGTTAGATT GTGAATCTGA ATCTGAGGAT CAGAGTAAAG GAGGAAGTGA GTTAGATGAG 
TTAGCAAATG GAGAAGTAGG AGGTGACCGG AGTGAGAAAA TGAAAAATCA GATTAAGCAG 
ATGATGGACA TTTCTCGGAC ACAAACTGCA ATATCAGTAG TTGAAGAGGA TCTAAAACTT 
CTGCAGATTA AGCTTAGAGC CTCAATGTCT ACTAAATGCA ACCTGGAAGA CCAAATAAAG 
AAATTAGAAG ATGACCGTGA TTCACTGCAG TCTGCCAAAG CCGGCCTGGA GGACGAATGC 
AAAACCCTGA GGCAAAAAGT GGAGATTCTG AACGAACTCT ATCAACAGAA GGAAATGGCT 
CTGCAAAAGA AACTGAGTCA GGAAGAGTAT GAGCGGCAGG AGAGAGAGCA GCGGCTGTCA 
GCTGCAGACG AGAAGGCGCT TCTGGCTGCG GAGGAAGTAA AAACGTACAA GCGTAGAATC 
GAAGAGATGG AGGATGAGTT GCAGAAGACA GAGCGCTCGT TTAAAAACCA GATTGCGACT 
CATGAGAAGA AAGCTCATGA TAACTGGCTC AAAGCTCGTG CTGCAGAAAG AGCTATAGCC 
GAGGAGAAGA GGGAAGCTGC CAACTTGAGA CACAAGTTAC TGGAATTAAC CCAGAAGATG 
GCAATGCTGC AAGAGGAGCC TGTGATTGTG AAGCCGAGGC CGGGCCGACC CAGCGCCCAG 
AACCCTCCGT GGAGAGGTCC TTTGAGCCAG AACGGCTCTT TTGGTCCATC CCCTGTGAGT 
GGGGGGGAGT GCTCCCCACC TCCCACGGCT GACCTGCCTG CGAGACCGCT CTCTGCTACG 
CTGAATCGAA GAGAGATGCC TAGAGGTGAA TTTGGGTCAG TGGACGGGCC TCTACCTCGT 
CCTCGTTGGT CATCTGAGGC ATCTGGGAAA CCCTCTGCCT CTGATCTGGG ATCTGGTGCA 
GCTCCCACGA TGAACAGCAG CTCGAGAAGC TCTTCCCCTA CCAAGGTGAC GGATGAAGGC 
AAGCAAACTG TTCCCCAAGA CCCCGAAGGC CCTTCAGTTC CCAGCAGTTC ACCTGTGGCT 
GAGCAGTCGG TAGTAGTTCC TATGGCTGCA AAAGGGCCGC CTCCTTTCCC AGGGGTGCCC 
CTCATGGGCT CCCCCGTGGG CGGCCCTCTA CCACCACCCA TTCGCTACGG ACCACCCCCT 
CAACTTTGCG GGCCTTTCGG GCCTCGGCCG CTTCCTCCAC CATTTGGTCC TGCTTTACGT 
CCACCACTAG GCTTAAGAGA ATACGCACCA GGCATTCCAC CTGGAAAACG GGACCTGTCT 
CTTGATCCTC GAGAATTTTT ACCGGGACAT GCACCTTTTA GACCTTTAGG CTCTTTTGGC 
CCAAGAGAGT ACTTTATTCC CGGTACCCGA CTACCACCCC CTGCCCATGG TCCCCAGGAT 
TATCCACCTC CACCTGCTGC AAGAGACTTA CTGCCTTCTG GCTCCAGAGA CGAGCCTCCA 
CCTGCCTCCC AGGGCAGTAG CCAGGACTGT TCACAGGCTT TAAAACAGAG CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013966638.1
CDS	68..5824
Translation	MYRGEALEDFIGPDCRFVSFKKGDAVYVYYKLAGRPPEVWAGSVGRIFGYFPKDLIQVVHEYTKEELQVPTDETDFVCFDGGRDDFDNYNVEELLGFLELHNSATEESEKAIEETQYEEDTPPEVLEESDLELEPVEVNSEESESVASENTKELKERSGAQKNHPHVNSPADHAQGDPSSFEPFEEMLQDKLKVPESENNKTSNGSQVSNEQQTIDAYKLLKKEMTLDLKTKFGSTADALVSDDETTRLVTSLEDDFDEELDAEYHIVGKDEEENEDNFDELPLLTFAHGGEDVKTPVKSGVEKYSAESEQSSNEEDKVEAPQPPGIKNYDKDILTTWEDTVFFTEDDSVTSMDLGSSESQEGKEGDDAFIPESKWRKPRSAADYIDPKKAEDGLLPTANDVGTKTDNDEDLEIHIAGKGRGAKEPKPGLAQAEMGPEEEKQEGMTTHGSTHSGDLGSLPSTGKGKEMLESAFDNKEDDLKGAAIHISKGMLHEEKPGEQISEGGSEREPLPQATGGQVNEEKPDQESVGIAAPSGGGQHNASRDRVEDVHALAGLKPHTPSVEHPKEEFREERLLKTQNQPRPSPLDGVGLPGELKEEGPIWGRNLSWPQGGEVAAAHSKQMDEKGRLAEEEVREDSSAEELAPQQPKAGSQEVEPTGRPGSPEVPGVHTEKPEAEDDHSPEELLEDENALSAKQAKEKSPGIQGRWLDVNLQSPSRAPLGVVNTDAEMEGNKEEASTILEIERKGETFGKEVEPGGRGREAGSGVVETESPLAGAKAQTASGGSDSPDKKNRTPGLGEMFQNQDSDYFKEDSAEKSAQKPGVEELSEEDQDFERPADTQSQGPAPEEQEGGPLRGTPPTAVRPMRSDYVKDLPIIGSFFKEQQSLQRFQKYFDVHELEAMFHEMSLKLKLAQQESLPYNVEKVLDKVFRASESQILSTAEKMLDTRVAENRDLGMMESNIFEEAAVLDDIQDLIYFVRYRHSTMEETAPLATGQPPQEGWGGTLEGMQPHLEDNFPQGHTKDNFPQDHIEDSFPQDHTEDSFPQDHTEDNFPQDHTEDLQIQILKEPSQLDQPVVGDMGASEVSQQPNTEKDGDPGIITTESSPVDADDAKKQLQTNAEEPASVTPLENAILLIYSFIFYLTKTLVATLPDDVQPGPDFYGLPWKPVLLTAFLGIGSFAVFFWKTVLVVKSRVYQVTEQQISEKLKNIMKENAELVQKLSNYEQKIKESKKHVQETKKQNIILSDEAIKFKDKIKKLEETNEILDDTAKNLRVMLESEREQNAKNQDLILENKKSIEKLKDVISVNASEFSEVQIALNEAKLSEEKVKSECHRVQEENARLKKKKEQLQQEIKDWSKSHAELSEQIKSFETSQKNLEVALTHKDDNINALTNCITQLNRLDCESESEDQSKGGSELDELANGEVGGDRSEKMKNQIKQMMDISRTQTAISVVEEDLKLLQIKLRASMSTKCNLEDQIKKLEDDRDSLQSAKAGLEDECKTLRQKVEILNELYQQKEMALQKKLSQEEYERQEREQRLSAADEKALLAAEEVKTYKRRIEEMEDELQKTERSFKNQIATHEKKAHDNWLKARAAERAIAEEKREAANLRHKLLELTQKMAMLQEEPVIVKPRPGRPSAQNPPWRGPLSQNGSFGPSPVSGGECSPPPTADLPARPLSATLNRREMPRGEFGSVDGPLPRPRWSSEASGKPSASDLGSGAAPTMNSSSRSSSPTKVTDEGKQTVPQDPEGPSVPSSSPVAEQSVVVPMAAKGPPPFPGVPLMGSPVGGPLPPPIRYGPPPQLCGPFGPRPLPPPFGPALRPPLGLREYAPGIPPGKRDLSLDPREFLPGHAPFRPLGSFGPREYFIPGTRLPPPAHGPQDYPPPPAARDLLPSGSRDEPPPASQGSSQDCSQALKQSP

Target ORF information:

RefSeq Version	XM_014111163.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris melanoma inhibitory activity family, member 3 (MIA3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014111163.1

ORF Insert Sequence:

ATGTACCGAG GTGAGGCTCT AGAAGATTTT ATAGGCCCAG ATTGTCGTTT CGTGAGTTTT 
AAAAAAGGTG ATGCTGTGTA TGTCTACTAT AAACTGGCAG GGAGACCGCC TGAAGTTTGG 
GCCGGAAGTG TTGGGCGTAT TTTTGGATAT TTTCCAAAGG ATTTAATACA AGTAGTCCAT 
GAATATACTA AAGAGGAGCT ACAGGTTCCA ACAGATGAGA CAGATTTTGT TTGTTTTGAT 
GGGGGAAGAG ATGATTTTGA TAATTATAAT GTAGAAGAAC TTTTAGGATT TTTGGAATTA 
CACAATTCTG CAACTGAAGA GTCTGAGAAA GCCATAGAAG AAACTCAGTA TGAGGAGGAC 
ACCCCTCCTG AAGTATTGGA GGAAAGCGAC CTCGAACTAG AACCAGTAGA AGTTAACTCA 
GAGGAGAGTG AAAGTGTAGC CTCAGAAAAC ACTAAGGAAC TCAAGGAAAG ATCTGGGGCT 
CAGAAGAACC ACCCGCATGT AAACAGTCCA GCAGATCATG CTCAGGGAGA TCCATCTTCA 
TTTGAACCTT TTGAGGAAAT GCTACAAGAT AAACTAAAAG TGCCAGAAAG TGAAAACAAC 
AAAACCAGCA ATGGTTCTCA GGTCTCGAAT GAACAGCAGA CAATTGATGC TTATAAACTT 
TTGAAAAAAG AAATGACCCT GGACTTAAAA ACCAAATTTG GCTCCACTGC TGATGCACTT 
GTATCTGATG ATGAGACAAC CAGACTTGTT ACTTCATTAG AAGATGATTT TGATGAGGAA 
TTGGATGCTG AGTATCACAT AGTTGGCAAG GACGAAGAAG AGAATGAAGA CAACTTTGAC 
GAGCTGCCGT TACTGACCTT CGCACATGGT GGAGAAGATG TGAAAACTCC AGTGAAGTCC 
GGAGTTGAGA AATACTCAGC AGAGAGCGAG CAGAGTTCAA ATGAAGAGGA TAAGGTTGAG 
GCACCTCAGC CCCCTGGCAT CAAAAACTAT GATAAAGATA TATTAACAAC CTGGGAGGAT 
ACTGTCTTCT TCACAGAAGA TGACAGCGTA ACAAGTATGG ATTTGGGGAG TTCTGAATCA 
CAGGAAGGCA AGGAAGGTGA TGATGCGTTC ATCCCAGAGA GCAAATGGAG GAAACCACGG 
TCAGCAGCGG ATTACATTGA CCCTAAGAAG GCAGAAGATG GTCTTTTGCC TACAGCTAAC 
GATGTAGGTA CTAAAACAGA TAACGACGAA GACCTGGAAA TTCACATTGC AGGAAAAGGG 
AGGGGAGCCA AGGAACCCAA GCCGGGCCTG GCACAGGCCG AGATGGGGCC GGAGGAGGAG 
AAGCAGGAAG GCATGACCAC ACACGGTTCT ACTCATAGTG GTGACCTCGG CTCTTTGCCA 
TCTACTGGAA AGGGTAAAGA AATGTTGGAA TCAGCATTTG ATAACAAAGA AGATGACCTA 
AAAGGAGCGG CTATTCATAT TTCAAAAGGA ATGCTCCATG AAGAAAAGCC CGGAGAGCAG 
ATTTCAGAAG GGGGCTCGGA GCGTGAACCC CTCCCTCAAG CCACAGGGGG TCAAGTGAAC 
GAAGAAAAGC CTGACCAGGA GTCTGTGGGT ATCGCAGCAC CCTCTGGGGG TGGTCAGCAT 
AATGCCTCCA GGGACCGTGT GGAAGACGTC CACGCTTTAG CAGGATTGAA ACCACATACA 
CCGTCAGTGG AGCATCCAAA GGAGGAATTT AGAGAGGAAC GGCTTCTTAA AACTCAGAAT 
CAACCTAGGC CCTCCCCTCT GGATGGAGTT GGTTTGCCAG GAGAACTGAA AGAAGAGGGT 
CCCATTTGGG GAAGAAATCT TTCCTGGCCG CAAGGAGGAG AAGTGGCTGC TGCACATAGT 
AAGCAGATGG ATGAGAAGGG GAGGCTTGCC GAGGAGGAGG TCAGAGAGGA CTCCTCAGCT 
GAAGAGTTAG CTCCTCAACA GCCCAAGGCG GGCTCCCAGG AAGTGGAGCC CACAGGCCGG 
CCAGGTAGCC CGGAAGTGCC AGGTGTCCAT ACTGAGAAAC CAGAGGCAGA AGACGATCAC 
AGCCCTGAAG AACTCCTGGA GGATGAAAAT GCTCTCAGCG CAAAACAGGC TAAAGAAAAA 
AGCCCCGGGA TTCAGGGCAG GTGGTTAGAC GTTAACCTGC AGTCCCCCTC AAGAGCTCCT 
TTAGGTGTGG TTAATACCGA TGCAGAAATG GAAGGAAACA AAGAAGAAGC TAGTACTATT 
TTGGAAATAG AAAGAAAAGG TGAAACTTTT GGCAAAGAGG TAGAGCCAGG GGGCAGGGGC 
AGGGAAGCAG GTAGTGGGGT GGTGGAAACA GAAAGCCCTC TTGCAGGTGC AAAAGCACAG 
ACGGCATCTG GAGGAAGTGA CTCTCCTGAC AAGAAAAATC GGACTCCGGG GTTAGGTGAA 
ATGTTTCAGA ATCAAGACTC TGATTATTTT AAAGAAGACA GCGCTGAGAA ATCTGCACAG 
AAGCCTGGGG TAGAAGAACT CTCTGAAGAG GACCAGGACT TTGAGAGACC CGCGGACACA 
CAGAGCCAGG GCCCTGCCCC CGAGGAGCAG GAAGGTGGCC CGCTGCGCGG GACGCCACCC 
ACGGCGGTGA GGCCAATGCG TAGCGACTAC GTGAAGGACT TGCCCATAAT TGGCAGCTTC 
TTTAAAGAAC AACAGTCTCT GCAGAGGTTC CAGAAGTACT TCGATGTCCA CGAGCTGGAA 
GCCATGTTCC ACGAAATGTC ATTAAAGCTG AAGTTGGCGC AGCAGGAGAG CCTGCCTTAT 
AACGTGGAGA AAGTCCTAGA TAAGGTCTTC CGTGCATCTG AGTCACAGAT TCTGAGCACA 
GCAGAGAAAA TGCTTGATAC CCGTGTGGCT GAAAACAGAG ATCTGGGAAT GATGGAAAGT 
AACATATTTG AGGAGGCAGC CGTACTAGAC GATATTCAAG ACCTGATCTA TTTTGTCAGG 
TACAGACACT CCACCATGGA GGAGACTGCC CCGCTGGCCA CGGGACAGCC CCCCCAGGAA 
GGCTGGGGTG GCACGTTGGA AGGGATGCAG CCACACCTTG AAGATAATTT CCCACAAGGT 
CACACCAAAG ATAATTTCCC ACAAGACCAC ATAGAAGACA GTTTCCCACA AGATCACACA 
GAAGACAGTT TCCCACAAGA TCACACAGAA GACAATTTCC CACAAGATCA CACAGAAGAC 
CTTCAAATAC AGATTCTCAA AGAGCCCAGC CAGTTGGATC AACCTGTGGT TGGTGACATG 
GGTGCCTCAG AAGTGTCACA GCAGCCAAAT ACTGAGAAAG ATGGAGACCC AGGGATAATT 
ACAACAGAAA GCAGTCCTGT TGATGCTGAT GATGCAAAAA AGCAACTACA AACCAATGCT 
GAAGAGCCCG CAAGCGTCAC ACCTTTGGAA AATGCAATTC TTTTAATATA TTCATTCATA 
TTTTATTTAA CTAAGACGTT AGTTGCTACA TTGCCTGATG ACGTTCAGCC TGGGCCTGAT 
TTTTATGGCT TGCCATGGAA GCCTGTACTT CTCACTGCCT TCTTGGGAAT TGGTTCATTT 
GCGGTCTTCT TCTGGAAAAC TGTCCTTGTT GTAAAGAGTA GAGTCTATCA AGTCACTGAA 
CAACAAATTT CTGAGAAGTT GAAGAACATC ATGAAAGAAA ATGCAGAACT TGTACAAAAA 
TTGTCAAATT ATGAACAGAA GATTAAGGAA TCAAAAAAAC ATGTTCAAGA AACCAAGAAA 
CAAAATATAA TTCTGTCTGA TGAAGCAATT AAATTTAAGG ATAAAATCAA GAAACTTGAA 
GAAACAAATG AAATTCTGGA CGATACAGCT AAAAATCTGC GTGTTATGTT AGAATCTGAG 
AGAGAACAGA ATGCCAAGAA TCAGGACTTG ATACTGGAAA ACAAGAAATC TATAGAGAAG 
CTGAAGGATG TTATTTCAGT GAATGCCTCA GAATTTTCAG AGGTTCAAAT TGCCCTTAAT 
GAAGCTAAAC TTAGTGAAGA GAAGGTGAAG TCTGAATGCC ATCGAGTTCA AGAAGAAAAT 
GCTAGGCTTA AGAAGAAGAA GGAACAGTTG CAGCAAGAAA TCAAAGATTG GAGCAAATCA 
CACGCTGAGC TCAGTGAGCA AATCAAATCA TTTGAGACAT CTCAGAAAAA TTTGGAAGTA 
GCTCTTACTC ACAAAGATGA TAATATTAAT GCTTTGACTA ATTGCATTAC CCAGTTGAAT 
CGGTTAGATT GTGAATCTGA ATCTGAGGAT CAGAGTAAAG GAGGAAGTGA GTTAGATGAG 
TTAGCAAATG GAGAAGTAGG AGGTGACCGG AGTGAGAAAA TGAAAAATCA GATTAAGCAG 
ATGATGGACA TTTCTCGGAC ACAAACTGCA ATATCAGTAG TTGAAGAGGA TCTAAAACTT 
CTGCAGATTA AGCTTAGAGC CTCAATGTCT ACTAAATGCA ACCTGGAAGA CCAAATAAAG 
AAATTAGAAG ATGACCGTGA TTCACTGCAG TCTGCCAAAG CCGGCCTGGA GGACGAATGC 
AAAACCCTGA GGCAAAAAGT GGAGATTCTG AACGAACTCT ATCAACAGAA GGAAATGGCT 
CTGCAAAAGA AACTGAGTCA GGAAGAGTAT GAGCGGCAGG AGAGAGAGCA GCGGCTGTCA 
GCTGCAGACG AGAAGGCGCT TCTGGCTGCG GAGGAAGTAA AAACGTACAA GCGTAGAATC 
GAAGAGATGG AGGATGAGTT GCAGAAGACA GAGCGCTCGT TTAAAAACCA GATTGCGACT 
CATGAGAAGA AAGCTCATGA TAACTGGCTC AAAGCTCGTG CTGCAGAAAG AGCTATAGCC 
GAGGAGAAGA GGGAAGCTGC CAACTTGAGA CACAAGTTAC TGGAATTAAC CCAGAAGATG 
GCAATGCTGC AAGAGGAGCC TGTGATTGTG AAGCCGAGGC CGGGCCGACC CAGCGCCCAG 
AACCCTCCGT GGAGAGGTCC TTTGAGCCAG AACGGCTCTT TTGGTCCATC CCCTGTGAGT 
GGGGGGGAGT GCTCCCCACC TCCCACGGCT GACCTGCCTG CGAGACCGCT CTCTGCTACG 
CTGAATCGAA GAGAGATGCC TAGAGGTGAA TTTGGGTCAG TGGACGGGCC TCTACCTCGT 
CCTCGTTGGT CATCTGAGGC ATCTGGGAAA CCCTCTGCCT CTGATCTGGG ATCTGGTGCA 
GCTCCCACGA TGAACAGCAG CTCGAGAAGC TCTTCCCCTA CCAAGGTGAC GGATGAAGGC 
AAGCAAACTG TTCCCCAAGA CCCCGAAGGC CCTTCAGTTC CCAGCAGTTC ACCTGTGGCT 
GAGCAGTCGG TAGTAGTTCC TATGGCTGCA AAAGGGCCGC CTCCTTTCCC AGGGGTGCCC 
CTCATGGGCT CCCCCGTGGG CGGCCCTCTA CCACCACCCA TTCGCTACGG ACCACCCCCT 
CAACTTTGCG GGCCTTTCGG GCCTCGGCCG CTTCCTCCAC CATTTGGTCC TGCTTTACGT 
CCACCACTAG GCTTAAGAGA ATACGCACCA GGCATTCCAC CTGGAAAACG GGACCTGTCT 
CTTGATCCTC GAGAATTTTT ACCGGGACAT GCACCTTTTA GACCTTTAGG CTCTTTTGGC 
CCAAGAGAGT ACTTTATTCC CGGTACCCGA CTACCACCCC CTGCCCATGG TCCCCAGGAT 
TATCCACCTC CACCTGCTGC AAGAGACTTA CTGCCTTCTG GCTCCAGAGA CGAGCCTCCA 
CCTGCCTCCC AGGGCAGTAG CCAGGACTGT TCACAGGCTT TAAAACAGAG CCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MIA3
Entrez Gene ID	100855882
Full Name	MIA family member 3, ER export factor
General protein information	Preferred Names MIA family member 3, ER export factor Names transport and Golgi organization protein 1 homolog melanoma inhibitory activity family, member 3
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

Mus musculus (house mouse)	Mia3	NP_796363.2
Rattus norvegicus (Norway rat)	Mia3	XP_006250418.1
	MIA3	XP_001100730.1
Bos taurus (cattle)	MIA3	NP_001160043.1
Homo sapiens (human)	MIA3	NP_940953.2
Canis lupus familiaris (dog)	MIA3	XP_005640892.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MIA3 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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