mec-10 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	mec-10
Entrez Gene ID	181101
Full Name	Degenerin mec-10
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_077037.4	NP_509438.1	Degenerin mec-10
NM_077037.6	NP_509438.1	Degenerin mec-10

Pathways from BioSystems

Homologies

	SCNN1G	XP_001093466.1
Mus musculus (house mouse)	Scnn1g	NP_035456.1
Caenorhabditis elegans (roundworm)	asic-1	NP_491214.3
Caenorhabditis elegans (roundworm)	mec-4	NP_510712.2
Caenorhabditis elegans (roundworm)	del-1	NP_510380.1
Caenorhabditis elegans (roundworm)	unc-8	NP_501138.1
Xenopus tropicalis (tropical clawed frog)	scnn1g	XP_004918046.1
Pan troglodytes (chimpanzee)	SCNN1G	XP_003315070.1
Caenorhabditis elegans (roundworm)	unc-105	NP_001254142.1
Caenorhabditis elegans (roundworm)	asic-2	NP_492099.1
Caenorhabditis elegans (roundworm)	mec-10	NP_509438.1
Bos taurus (cattle)	SCNN1G	NP_001180103.1
Gallus gallus (chicken)	SCNN1G	XP_414880.2
Homo sapiens (human)	SCNN1G	NP_001030.2
Rattus norvegicus (Norway rat)	Scnn1g	NP_058742.1
Canis lupus familiaris (dog)	SCNN1G	XP_547093.2
Caenorhabditis elegans (roundworm)	deg-1	NP_509311.1
Caenorhabditis elegans (roundworm)	del-4	NP_492230.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

LSS response was largely abolished by MEC-10 mutations encoded by a touch-insensitive mec-10 allele, providing a correlation between the channel's responses to two different mechanical forces
Title: Activation of the Caenorhabditis elegans Degenerin Channel by Shear Stress Requires the MEC-10 Subunit.

MEC-10, but not MEC-6, forms puncta in touch receptor neurites that colocalize with MEC-4 when MEC-4 is overexpressed in the touch receptor neurons
Title: Subunit composition of a DEG/ENaC mechanosensory channel of Caenorhabditis elegans.

This study suggested that mutant MEC-10 proteins may affect channel current via MEC-2.
Title: The DEG/ENaC protein MEC-10 regulates the transduction channel complex in Caenorhabditis elegans touch receptor neurons.

MEC-10 protein is asymmetrically distributed within the touch neuron process. It may contribute to only a subset of gentle touch mechanosensory complexes found preferentially at the proximal dendrite.
Title: Spatial asymmetry in the mechanosensory phenotypes of the C. elegans DEG/ENaC gene mec-10.

harsh touch specifically required the DEG/ENaC proteins MEC-10 and DEGT-1 (F25D1.4), which represent putative components of a harsh touch mechanotransduction complex; responses to cold required the TRPA-1 channel and were MEC-10 and DEGT-1 independent
Title: Specific roles for DEG/ENaC and TRP channels in touch and thermosensation in C. elegans nociceptors.

The following mec-10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mec-10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa349708	NM_077037.6 Latest version!	Caenorhabditis elegans Degenerin mec-10 (mec-10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OCa349708	NM_077037.4	Caenorhabditis elegans Degenerin mec-10 (mec-10), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mec-10 ( NM_077037.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_509438.1 Caenorhabditis elegans Degenerin mec-10 (mec-10), partial mRNA.

Price & Availability↑

CloneID	OCa349708
Clone ID Related Accession (Same CDS sequence)	NM_077037.4 , NM_077037.6
Accession Version	NM_077037.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Degenerin mec-10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Jun 26, 2012 this sequence version replaced NM_077037.3. COMPLETENESS: incomplete on both ends.

ATGAATCGAA ACCCGCGAAT GTCAAAATTT CAACCAAATC CTCGTAGTCG CAGTCGATTT 
CAAGATGAAA CAGATTTGCG TTCGCTCAGA AGTTTCAAAA CCGATTTTTC AAATTATCTC 
GCAAGTGACA CTAACTTTCT GAATGTTGCG GAAATTATGA CATCTTATGC TTACGGAGAA 
AGCAATAATG CACACGAAAA AGAAATTCAG TGCGATCTTC TGACAGAAAA CGGTGGTATT 
GAAATTGACC CGACAAGATT ATCTTACAGA GAAAGAATAA GATGGCATTT ACAACAGTTT 
TGCTATAAAA CATCTAGTCA TGGTATTCCA ATGCTTGGGC AAGCTCCAAA CAGTTTATAC 
AGAGCTGCAT GGGTATTCTT GCTTCTAATC TGTGCTATAC AATTTATCAA TCAGGCGGTC 
GCTGTGATTC AGAAGTATCA GAAGATGGAT AAAATCACGG ATATACAATT GAAGTTTGAC 
ACAGCGCCCT TTCCTGCAAT TACTCTTTGC AATTTGAACC CGTACAAAGA TAGTGTCATC 
AGATCACATG ACAGTATTAG TAAAATCCTT GGTGTATTCA AAAGTGTTAT GAAAAAAGCT 
GGTGATAGCA GCTCGGAAGC ATTGGAAGAA GAGGAGGAAA CCGAATATGA TATGAATGGA 
ATAACTATCC AAGCAAAAAG AAAAAAGAGA GGAGCTGGCG AGAAGGGAAC ATTTGAACCA 
GCGAACTCGG CTTGTGAGTG CGACGAGGAA GACGGTTCCA ATGAATGTGA AGAAAGATCC 
ACTGAAAAGC CATCGGGCGA TAATGATATG TGCATTTGTG CTTTCGATCG GCAGACCAAC 
GATGCGTGGC CGTGTCATAG AAAAGAGCAG TGGACAAATA CTACATGTCA AACGTGTGAT 
GAGCATTATT TATGCAGCAA AAAAGCTAAG AAAGGAACCA AAAGATCGGA ACTCAAGAAG 
GAGCCGTGTA TTTGTGAGAG CAAGGGACTT TTCTGTATTA AGCACGAACA TGCTGCAATG 
GTATTGAATT TATGGGAATA CTTTGGCGAT AGTGAAGATT TCTCTGAAAT ATCCACAGAA 
GAAAGAGAAG CATTGGGATT TGGGAACATG ACAGATGAAG TTGCCATTGT AACAAAAGCC 
AAGGAAAATA TAATTTTCGC CATGAGTGCT CTCTCGGAAG AGCAAAGAAT ACTAATGTCG 
CAAGCTAAAC ACAATTTGAT CCATAAATGC TCATTCAATG GAAAACCATG TGATATCGAC 
CAAGATTTTG AGCTAGTTGC AGACCCTACA TTTGGAAACT GTTTTGTTTT TAATCACGAC 
AGAGAGATTT TCAAGTCCAG TGTGAGAGCT GGACCACAAT ATGGCTTGCG CGTAATGCTA 
TTTGTAAACG CGAGCGACTA TCTGCCAACT TCCGAAGCCG TCGGCATACG ATTGACCATT 
CATGACAAAG ACGATTTCCC ATTCCCGGAC ACTTTCGGCT ACTCGGCACC AACCGGTTAC 
ATCTCGTCGT TCGGCATGCG CATGAAGAAG ATGAGTAGAT TACCTGCGCC GTATGGGGAT 
TGTGTAGAGG ATGGTGCCAC GTCAAACTAC ATCTACAAGG GTTATGCATA CTCAACAGAA 
GGTTGCTATC GTACTTGTTT TCAAGAACTG ATTATTGATA GATGCGGATG CAGTGATCCA 
CGATTTCCGT CGATCGGGGG TGTTCAACCG TGTCAAGTGT TCAATAAAAA CCACAGAGAG 
TGCCTTGAAA AGCACACACA TCAAATTGGA GAAATTCACG GATCATTCAA GTGTCGGTGC 
CAACAGCCAT GCAATCAAAC TATTTACACA ACTTCTTATT CTGAAGCCAT TTGGCCATCC 
CAAGCATTAA ACATCAGTCT GGGACAGTGC GAAAAAGAAG CGGAGGAATG CAATGAGGAA 
TATAAAGAAA ACGCGGCAAT GTTAGAGGTT TTCTACGAGG CACTAAACTT TGAAGTTCTA 
TCAGAGTCTG AAGCTTATGG TATTGTAAAA ATGATGGCTG ATTTTGGAGG ACACCTTGGA 
CTTTGGTCAG GAGTTTCTGT TATGACTTGT TGTGAGTTTG TGTGTCTAGC CTTTGAGCTG 
ATATACATGG CAATTGCACA TCACATAAAC CAGCAGAGAA TTCGACGACG AGAAAATGCT 
GCAAATGAGT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_509438.1
CDS	1..2175
Translation	MNRNPRMSKFQPNPRSRSRFQDETDLRSLRSFKTDFSNYLASDTNFLNVAEIMTSYAYGESNNAHEKEIQCDLLTENGGIEIDPTRLSYRERIRWHLQQFCYKTSSHGIPMLGQAPNSLYRAAWVFLLLICAIQFINQAVAVIQKYQKMDKITDIQLKFDTAPFPAITLCNLNPYKDSVIRSHDSISKILGVFKSVMKKAGDSSSEALEEEEETEYDMNGITIQAKRKKRGAGEKGTFEPANSACECDEEDGSNECEERSTEKPSGDNDMCICAFDRQTNDAWPCHRKEQWTNTTCQTCDEHYLCSKKAKKGTKRSELKKEPCICESKGLFCIKHEHAAMVLNLWEYFGDSEDFSEISTEEREALGFGNMTDEVAIVTKAKENIIFAMSALSEEQRILMSQAKHNLIHKCSFNGKPCDIDQDFELVADPTFGNCFVFNHDREIFKSSVRAGPQYGLRVMLFVNASDYLPTSEAVGIRLTIHDKDDFPFPDTFGYSAPTGYISSFGMRMKKMSRLPAPYGDCVEDGATSNYIYKGYAYSTEGCYRTCFQELIIDRCGCSDPRFPSIGGVQPCQVFNKNHRECLEKHTHQIGEIHGSFKCRCQQPCNQTIYTTSYSEAIWPSQALNISLGQCEKEAEECNEEYKENAAMLEVFYEALNFEVLSESEAYGIVKMMADFGGHLGLWSGVSVMTCCEFVCLAFELIYMAIAHHINQQRIRRRENAANEY

Target ORF information:

RefSeq Version	NM_077037.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Degenerin mec-10 (mec-10), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_077037.4

ORF Insert Sequence:

ATGAATCGAA ACCCGCGAAT GTCAAAATTT CAACCAAATC CTCGTAGTCG CAGTCGATTT 
CAAGATGAAA CAGATTTGCG TTCGCTCAGA AGTTTCAAAA CCGATTTTTC AAATTATCTC 
GCAAGTGACA CTAACTTTCT GAATGTTGCG GAAATTATGA CATCTTATGC TTACGGAGAA 
AGCAATAATG CACACGAAAA AGAAATTCAG TGCGATCTTC TGACAGAAAA CGGTGGTATT 
GAAATTGACC CGACAAGATT ATCTTACAGA GAAAGAATAA GATGGCATTT ACAACAGTTT 
TGCTATAAAA CATCTAGTCA TGGTATTCCA ATGCTTGGGC AAGCTCCAAA CAGTTTATAC 
AGAGCTGCAT GGGTATTCTT GCTTCTAATC TGTGCTATAC AATTTATCAA TCAGGCGGTC 
GCTGTGATTC AGAAGTATCA GAAGATGGAT AAAATCACGG ATATACAATT GAAGTTTGAC 
ACAGCGCCCT TTCCTGCAAT TACTCTTTGC AATTTGAACC CGTACAAAGA TAGTGTCATC 
AGATCACATG ACAGTATTAG TAAAATCCTT GGTGTATTCA AAAGTGTTAT GAAAAAAGCT 
GGTGATAGCA GCTCGGAAGC ATTGGAAGAA GAGGAGGAAA CCGAATATGA TATGAATGGA 
ATAACTATCC AAGCAAAAAG AAAAAAGAGA GGAGCTGGCG AGAAGGGAAC ATTTGAACCA 
GCGAACTCGG CTTGTGAGTG CGACGAGGAA GACGGTTCCA ATGAATGTGA AGAAAGATCC 
ACTGAAAAGC CATCGGGCGA TAATGATATG TGCATTTGTG CTTTCGATCG GCAGACCAAC 
GATGCGTGGC CGTGTCATAG AAAAGAGCAG TGGACAAATA CTACATGTCA AACGTGTGAT 
GAGCATTATT TATGCAGCAA AAAAGCTAAG AAAGGAACCA AAAGATCGGA ACTCAAGAAG 
GAGCCGTGTA TTTGTGAGAG CAAGGGACTT TTCTGTATTA AGCACGAACA TGCTGCAATG 
GTATTGAATT TATGGGAATA CTTTGGCGAT AGTGAAGATT TCTCTGAAAT ATCCACAGAA 
GAAAGAGAAG CATTGGGATT TGGGAACATG ACAGATGAAG TTGCCATTGT AACAAAAGCC 
AAGGAAAATA TAATTTTCGC CATGAGTGCT CTCTCGGAAG AGCAAAGAAT ACTAATGTCG 
CAAGCTAAAC ACAATTTGAT CCATAAATGC TCATTCAATG GAAAACCATG TGATATCGAC 
CAAGATTTTG AGCTAGTTGC AGACCCTACA TTTGGAAACT GTTTTGTTTT TAATCACGAC 
AGAGAGATTT TCAAGTCCAG TGTGAGAGCT GGACCACAAT ATGGCTTGCG CGTAATGCTA 
TTTGTAAACG CGAGCGACTA TCTGCCAACT TCCGAAGCCG TCGGCATACG ATTGACCATT 
CATGACAAAG ACGATTTCCC ATTCCCGGAC ACTTTCGGCT ACTCGGCACC AACCGGTTAC 
ATCTCGTCGT TCGGCATGCG CATGAAGAAG ATGAGTAGAT TACCTGCGCC GTATGGGGAT 
TGTGTAGAGG ATGGTGCCAC GTCAAACTAC ATCTACAAGG GTTATGCATA CTCAACAGAA 
GGTTGCTATC GTACTTGTTT TCAAGAACTG ATTATTGATA GATGCGGATG CAGTGATCCA 
CGATTTCCGT CGATCGGGGG TGTTCAACCG TGTCAAGTGT TCAATAAAAA CCACAGAGAG 
TGCCTTGAAA AGCACACACA TCAAATTGGA GAAATTCACG GATCATTCAA GTGTCGGTGC 
CAACAGCCAT GCAATCAAAC TATTTACACA ACTTCTTATT CTGAAGCCAT TTGGCCATCC 
CAAGCATTAA ACATCAGTCT GGGACAGTGC GAAAAAGAAG CGGAGGAATG CAATGAGGAA 
TATAAAGAAA ACGCGGCAAT GTTAGAGGTT TTCTACGAGG CACTAAACTT TGAAGTTCTA 
TCAGAGTCTG AAGCTTATGG TATTGTAAAA ATGATGGCTG ATTTTGGAGG ACACCTTGGA 
CTTTGGTCAG GAGTTTCTGT TATGACTTGT TGTGAGTTTG TGTGTCTAGC CTTTGAGCTG 
ATATACATGG CAATTGCACA TCACATAAAC CAGCAGAGAA TTCGACGACG AGAAAATGCT 
GCAAATGAGT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

mec-10 ( NM_077037.6 ) cDNA ORF clone, Caenorhabditis elegans -> NP_509438.1 Caenorhabditis elegans Degenerin mec-10 (mec-10), mRNA.

Price & Availability↑

CloneID	OCa349708
Clone ID Related Accession (Same CDS sequence)	NM_077037.4 , NM_077037.6
Accession Version	NM_077037.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2175bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Degenerin mec-10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003284). On Oct 18, 2019 this sequence version replaced NM_077037.5.

ATGAATCGAA ACCCGCGAAT GTCAAAATTT CAACCAAATC CTCGTAGTCG CAGTCGATTT 
CAAGATGAAA CAGATTTGCG TTCGCTCAGA AGTTTCAAAA CCGATTTTTC AAATTATCTC 
GCAAGTGACA CTAACTTTCT GAATGTTGCG GAAATTATGA CATCTTATGC TTACGGAGAA 
AGCAATAATG CACACGAAAA AGAAATTCAG TGCGATCTTC TGACAGAAAA CGGTGGTATT 
GAAATTGACC CGACAAGATT ATCTTACAGA GAAAGAATAA GATGGCATTT ACAACAGTTT 
TGCTATAAAA CATCTAGTCA TGGTATTCCA ATGCTTGGGC AAGCTCCAAA CAGTTTATAC 
AGAGCTGCAT GGGTATTCTT GCTTCTAATC TGTGCTATAC AATTTATCAA TCAGGCGGTC 
GCTGTGATTC AGAAGTATCA GAAGATGGAT AAAATCACGG ATATACAATT GAAGTTTGAC 
ACAGCGCCCT TTCCTGCAAT TACTCTTTGC AATTTGAACC CGTACAAAGA TAGTGTCATC 
AGATCACATG ACAGTATTAG TAAAATCCTT GGTGTATTCA AAAGTGTTAT GAAAAAAGCT 
GGTGATAGCA GCTCGGAAGC ATTGGAAGAA GAGGAGGAAA CCGAATATGA TATGAATGGA 
ATAACTATCC AAGCAAAAAG AAAAAAGAGA GGAGCTGGCG AGAAGGGAAC ATTTGAACCA 
GCGAACTCGG CTTGTGAGTG CGACGAGGAA GACGGTTCCA ATGAATGTGA AGAAAGATCC 
ACTGAAAAGC CATCGGGCGA TAATGATATG TGCATTTGTG CTTTCGATCG GCAGACCAAC 
GATGCGTGGC CGTGTCATAG AAAAGAGCAG TGGACAAATA CTACATGTCA AACGTGTGAT 
GAGCATTATT TATGCAGCAA AAAAGCTAAG AAAGGAACCA AAAGATCGGA ACTCAAGAAG 
GAGCCGTGTA TTTGTGAGAG CAAGGGACTT TTCTGTATTA AGCACGAACA TGCTGCAATG 
GTATTGAATT TATGGGAATA CTTTGGCGAT AGTGAAGATT TCTCTGAAAT ATCCACAGAA 
GAAAGAGAAG CATTGGGATT TGGGAACATG ACAGATGAAG TTGCCATTGT AACAAAAGCC 
AAGGAAAATA TAATTTTCGC CATGAGTGCT CTCTCGGAAG AGCAAAGAAT ACTAATGTCG 
CAAGCTAAAC ACAATTTGAT CCATAAATGC TCATTCAATG GAAAACCATG TGATATCGAC 
CAAGATTTTG AGCTAGTTGC AGACCCTACA TTTGGAAACT GTTTTGTTTT TAATCACGAC 
AGAGAGATTT TCAAGTCCAG TGTGAGAGCT GGACCACAAT ATGGCTTGCG CGTAATGCTA 
TTTGTAAACG CGAGCGACTA TCTGCCAACT TCCGAAGCCG TCGGCATACG ATTGACCATT 
CATGACAAAG ACGATTTCCC ATTCCCGGAC ACTTTCGGCT ACTCGGCACC AACCGGTTAC 
ATCTCGTCGT TCGGCATGCG CATGAAGAAG ATGAGTAGAT TACCTGCGCC GTATGGGGAT 
TGTGTAGAGG ATGGTGCCAC GTCAAACTAC ATCTACAAGG GTTATGCATA CTCAACAGAA 
GGTTGCTATC GTACTTGTTT TCAAGAACTG ATTATTGATA GATGCGGATG CAGTGATCCA 
CGATTTCCGT CGATCGGGGG TGTTCAACCG TGTCAAGTGT TCAATAAAAA CCACAGAGAG 
TGCCTTGAAA AGCACACACA TCAAATTGGA GAAATTCACG GATCATTCAA GTGTCGGTGC 
CAACAGCCAT GCAATCAAAC TATTTACACA ACTTCTTATT CTGAAGCCAT TTGGCCATCC 
CAAGCATTAA ACATCAGTCT GGGACAGTGC GAAAAAGAAG CGGAGGAATG CAATGAGGAA 
TATAAAGAAA ACGCGGCAAT GTTAGAGGTT TTCTACGAGG CACTAAACTT TGAAGTTCTA 
TCAGAGTCTG AAGCTTATGG TATTGTAAAA ATGATGGCTG ATTTTGGAGG ACACCTTGGA 
CTTTGGTCAG GAGTTTCTGT TATGACTTGT TGTGAGTTTG TGTGTCTAGC CTTTGAGCTG 
ATATACATGG CAATTGCACA TCACATAAAC CAGCAGAGAA TTCGACGACG AGAAAATGCT 
GCAAATGAGT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_509438.1
CDS	75..2249
Translation	MNRNPRMSKFQPNPRSRSRFQDETDLRSLRSFKTDFSNYLASDTNFLNVAEIMTSYAYGESNNAHEKEIQCDLLTENGGIEIDPTRLSYRERIRWHLQQFCYKTSSHGIPMLGQAPNSLYRAAWVFLLLICAIQFINQAVAVIQKYQKMDKITDIQLKFDTAPFPAITLCNLNPYKDSVIRSHDSISKILGVFKSVMKKAGDSSSEALEEEEETEYDMNGITIQAKRKKRGAGEKGTFEPANSACECDEEDGSNECEERSTEKPSGDNDMCICAFDRQTNDAWPCHRKEQWTNTTCQTCDEHYLCSKKAKKGTKRSELKKEPCICESKGLFCIKHEHAAMVLNLWEYFGDSEDFSEISTEEREALGFGNMTDEVAIVTKAKENIIFAMSALSEEQRILMSQAKHNLIHKCSFNGKPCDIDQDFELVADPTFGNCFVFNHDREIFKSSVRAGPQYGLRVMLFVNASDYLPTSEAVGIRLTIHDKDDFPFPDTFGYSAPTGYISSFGMRMKKMSRLPAPYGDCVEDGATSNYIYKGYAYSTEGCYRTCFQELIIDRCGCSDPRFPSIGGVQPCQVFNKNHRECLEKHTHQIGEIHGSFKCRCQQPCNQTIYTTSYSEAIWPSQALNISLGQCEKEAEECNEEYKENAAMLEVFYEALNFEVLSESEAYGIVKMMADFGGHLGLWSGVSVMTCCEFVCLAFELIYMAIAHHINQQRIRRRENAANEY

Target ORF information:

RefSeq Version	NM_077037.6
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Degenerin mec-10 (mec-10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_077037.6

ORF Insert Sequence:

ATGAATCGAA ACCCGCGAAT GTCAAAATTT CAACCAAATC CTCGTAGTCG CAGTCGATTT 
CAAGATGAAA CAGATTTGCG TTCGCTCAGA AGTTTCAAAA CCGATTTTTC AAATTATCTC 
GCAAGTGACA CTAACTTTCT GAATGTTGCG GAAATTATGA CATCTTATGC TTACGGAGAA 
AGCAATAATG CACACGAAAA AGAAATTCAG TGCGATCTTC TGACAGAAAA CGGTGGTATT 
GAAATTGACC CGACAAGATT ATCTTACAGA GAAAGAATAA GATGGCATTT ACAACAGTTT 
TGCTATAAAA CATCTAGTCA TGGTATTCCA ATGCTTGGGC AAGCTCCAAA CAGTTTATAC 
AGAGCTGCAT GGGTATTCTT GCTTCTAATC TGTGCTATAC AATTTATCAA TCAGGCGGTC 
GCTGTGATTC AGAAGTATCA GAAGATGGAT AAAATCACGG ATATACAATT GAAGTTTGAC 
ACAGCGCCCT TTCCTGCAAT TACTCTTTGC AATTTGAACC CGTACAAAGA TAGTGTCATC 
AGATCACATG ACAGTATTAG TAAAATCCTT GGTGTATTCA AAAGTGTTAT GAAAAAAGCT 
GGTGATAGCA GCTCGGAAGC ATTGGAAGAA GAGGAGGAAA CCGAATATGA TATGAATGGA 
ATAACTATCC AAGCAAAAAG AAAAAAGAGA GGAGCTGGCG AGAAGGGAAC ATTTGAACCA 
GCGAACTCGG CTTGTGAGTG CGACGAGGAA GACGGTTCCA ATGAATGTGA AGAAAGATCC 
ACTGAAAAGC CATCGGGCGA TAATGATATG TGCATTTGTG CTTTCGATCG GCAGACCAAC 
GATGCGTGGC CGTGTCATAG AAAAGAGCAG TGGACAAATA CTACATGTCA AACGTGTGAT 
GAGCATTATT TATGCAGCAA AAAAGCTAAG AAAGGAACCA AAAGATCGGA ACTCAAGAAG 
GAGCCGTGTA TTTGTGAGAG CAAGGGACTT TTCTGTATTA AGCACGAACA TGCTGCAATG 
GTATTGAATT TATGGGAATA CTTTGGCGAT AGTGAAGATT TCTCTGAAAT ATCCACAGAA 
GAAAGAGAAG CATTGGGATT TGGGAACATG ACAGATGAAG TTGCCATTGT AACAAAAGCC 
AAGGAAAATA TAATTTTCGC CATGAGTGCT CTCTCGGAAG AGCAAAGAAT ACTAATGTCG 
CAAGCTAAAC ACAATTTGAT CCATAAATGC TCATTCAATG GAAAACCATG TGATATCGAC 
CAAGATTTTG AGCTAGTTGC AGACCCTACA TTTGGAAACT GTTTTGTTTT TAATCACGAC 
AGAGAGATTT TCAAGTCCAG TGTGAGAGCT GGACCACAAT ATGGCTTGCG CGTAATGCTA 
TTTGTAAACG CGAGCGACTA TCTGCCAACT TCCGAAGCCG TCGGCATACG ATTGACCATT 
CATGACAAAG ACGATTTCCC ATTCCCGGAC ACTTTCGGCT ACTCGGCACC AACCGGTTAC 
ATCTCGTCGT TCGGCATGCG CATGAAGAAG ATGAGTAGAT TACCTGCGCC GTATGGGGAT 
TGTGTAGAGG ATGGTGCCAC GTCAAACTAC ATCTACAAGG GTTATGCATA CTCAACAGAA 
GGTTGCTATC GTACTTGTTT TCAAGAACTG ATTATTGATA GATGCGGATG CAGTGATCCA 
CGATTTCCGT CGATCGGGGG TGTTCAACCG TGTCAAGTGT TCAATAAAAA CCACAGAGAG 
TGCCTTGAAA AGCACACACA TCAAATTGGA GAAATTCACG GATCATTCAA GTGTCGGTGC 
CAACAGCCAT GCAATCAAAC TATTTACACA ACTTCTTATT CTGAAGCCAT TTGGCCATCC 
CAAGCATTAA ACATCAGTCT GGGACAGTGC GAAAAAGAAG CGGAGGAATG CAATGAGGAA 
TATAAAGAAA ACGCGGCAAT GTTAGAGGTT TTCTACGAGG CACTAAACTT TGAAGTTCTA 
TCAGAGTCTG AAGCTTATGG TATTGTAAAA ATGATGGCTG ATTTTGGAGG ACACCTTGGA 
CTTTGGTCAG GAGTTTCTGT TATGACTTGT TGTGAGTTTG TGTGTCTAGC CTTTGAGCTG 
ATATACATGG CAATTGCACA TCACATAAAC CAGCAGAGAA TTCGACGACG AGAAAATGCT 
GCAAATGAGT ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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オンライン注文

mec-10 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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