unc-8 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	unc-8
Entrez Gene ID	177494
Full Name	Degenerin unc-8
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_001307364.1	NP_001294293.1	Degenerin unc-8
NM_001307365.1	NP_001294294.1	Degenerin unc-8
NM_001307363.2	NP_001294292.1	Degenerin unc-8
NM_001307364.2	NP_001294293.1	Degenerin unc-8
NM_001307365.2	NP_001294294.1	Degenerin unc-8
NM_001307363.1	NP_001294292.1	Degenerin unc-8
NM_068737.1	NP_501138.1	Degenerin unc-8
NM_068737.2	NP_501138.1	Degenerin unc-8

Pathways from BioSystems

Homologies

	SCNN1G	XP_001093466.1
Mus musculus (house mouse)	Scnn1g	NP_035456.1
Caenorhabditis elegans (roundworm)	asic-1	NP_491214.3
Caenorhabditis elegans (roundworm)	mec-4	NP_510712.2
Caenorhabditis elegans (roundworm)	del-1	NP_510380.1
Caenorhabditis elegans (roundworm)	unc-8	NP_501138.1
Xenopus tropicalis (tropical clawed frog)	scnn1g	XP_004918046.1
Pan troglodytes (chimpanzee)	SCNN1G	XP_003315070.1
Caenorhabditis elegans (roundworm)	unc-105	NP_001254142.1
Caenorhabditis elegans (roundworm)	asic-2	NP_492099.1
Caenorhabditis elegans (roundworm)	mec-10	NP_509438.1
Bos taurus (cattle)	SCNN1G	NP_001180103.1
Gallus gallus (chicken)	SCNN1G	XP_414880.2
Homo sapiens (human)	SCNN1G	NP_001030.2
Rattus norvegicus (Norway rat)	Scnn1g	NP_058742.1
Canis lupus familiaris (dog)	SCNN1G	XP_547093.2
Caenorhabditis elegans (roundworm)	deg-1	NP_509311.1
Caenorhabditis elegans (roundworm)	del-4	NP_492230.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The authors propose that the depolarizing effect of UNC-8-dependent sodium import elevates intracellular calcium in a positive feedback loop involving the voltage-gated calcium channel UNC-2 and the calcium-activated phosphatase TAX-6/calcineurin to initiate a caspase-dependent mechanism that disassembles the presynaptic apparatus.
Title: The DEG/ENaC cation channel protein UNC-8 drives activity-dependent synapse removal in remodeling GABAergic neurons.

Neurotoxic UNC-8(G387E) and UNC-8(A586T)mutants encode hyperactivated channels blocked by extracellular calcium and magnesium.
Title: Neurotoxic unc-8 mutants encode constitutively active DEG/ENaC channels that are blocked by divalent cations.

The following unc-8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the unc-8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa343690	NM_068737.2 Latest version!	Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa353357	NM_001307363.2 Latest version!	Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa353358	NM_001307364.2 Latest version!	Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OCa353359	NM_001307365.2 Latest version!	Caenorhabditis elegans Degenerin unc-8 (unc-8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OCa353358	NM_001307364.1	Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa353359	NM_001307365.1	Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa353357	NM_001307363.1	Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
OCa343690	NM_068737.1	Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

unc-8 ( NM_068737.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_501138.1 Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.

Price & Availability↑

CloneID	OCa343690
Clone ID Related Accession (Same CDS sequence)	NM_068737.1 , NM_068737.2
Accession Version	NM_068737.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Degenerin unc-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGTCACCTT TGCTGACGTG GAACTTGATA TGTGTCAGTT CAAGGTGGTA CACAATTTTA 
TGTTTAAAAA ATAAAGTTAA GTTCTGGTTA GGAACAAGAC TCGTCCATGA ACCAGAATCA 
ATGGAGAGCA GGTCAAGTCC ATATATTAGA CCATCTCCAT ACGCTGGAGG AGTTCATCCA 
CACTTTGAAG AGGAAGATGA CAGGTCAAAA CTTCATGCAT CAGCTCTCTA CTCGGAACGC 
CGAACGTCAT CCCGGAAATC GTTAAGATCA CAAAAAATAG ACTATCACAC AACAACAATA 
AAATCCCTTT GGTTTGATTT TTGTGCCAGA ACTTCTTCCC ATGGAATACC ATATGTTGCA 
ACATCTTCAT TTTTTGGCCG ATATGTATGG GCGGCACTCT TCATGTGCAT GTTAATGGCA 
TTCTTATTAC AAACCTATTG GACAATGTCA GAATATTTGC AGTATAGGAC AATTATTGAA 
ATGCAGTTGC AGTTTGAAGC TGCTGCATTT CCCGCTGCAA CAGTTTGCAA TTTGAATGCT 
TTCAAATATA GCGAGCTCAC GCAGTATGAA GAAATTAAAG AGGGTTTTGA CTATTGGGAA 
CGGGTGATAA ATGCTCGAAT GATGAGTGAC AGTATGAAGC CAGGAGGTGA TATTCTAGAA 
GCAATCAGTG TCAGGAAAAA GCGATCAAAA AGTCGAGATC AATTACTTTT TCCAATTGAT 
GACGAAGACT TAGAGGGTGC AGTGTATCAG CCAGTCTTTG TGAGATGCAC CTGTATGAAT 
ATGGAACAAT GTGTACCAAA TAGGAATCCT TTAGAGGTTA ACGCCTCAAT TTGTATGTGC 
TTTGAAGACG TGACACGTGG CTTAATCTGG CCATGCTACC CAACTTCCGT ATGGACTGTA 
AAAAAATGTT CTGGTTGTTC AATTTCCAAT ACTTGTCCTG ACCCGGATGG TCCTAATGCT 
TCAAAGCAAA TCGCAAAACA TAACAGTCCA CTTCCATGTC TTTGCCAATC AATTTCTCAT 
CATTGTATGG TACATCCAAA GGACGAAATC CGATGGTGGA ACCCAAACAA TTATACAGTT 
TATTCTGTAA CTGAACCACC GACCACAGAA ATAACTGAAA CCGAAGAAGC TTTTGGATTG 
AGCGACCTCA AAGATGCGGG AGCCATCACA ACTCAAACAA AAGAAAATTT AATTTTCTTA 
GTGGCTGCTT TACCCCGTGA AACAAGAAGG AACCTGTCAT ACACCCTCAA TGAGTTTGTT 
CTTCGATGTT CGTTTAATTC GAAGGATTGT AGTATGGAGC GTGACTTTAA ACTTCACGTT 
GATCCTGAAT ACGGCAATTG CTACACATTT AACTTCAATG ATTCGGTGGA GTTGAAAAAC 
AGTAGAGCTG GTCCCATGTA TGGTCTCCGT CTATTATTAA ACGTGCATCA AAGCGATTAC 
ATGCCAACAA CAGAAGCAGC AGGTGTTCGA TTAGTTGTCC ATGAACAAGA TCAAGAACCG 
TTCCCTGATA CATTTGGATA CAGTGCTCCA ACTGGATTCA TTTCTTCATT TGGTCTTAAA 
ACGAAAGAAC TACATCGTCT CAGTGCTCCA TGGGGGAATT GCAGTGACAC ATTCCGACCG 
GTTCCATACA TTTACAACGA ACACTATTCT CCTGAAGGTT GCCATCGAAA TTGTTTCCAA 
TTGAAAGTAC TTGAAATATG TGGATGTGGA GATCCAAGAT TCCCTCTACC ATCTGAAGAG 
CATAGACATT GTAATGCGAA GTCAAAAATT GATCGTCAAT GTTTATCAAA CCTAACAAGT 
GACTCTGGAG GTTACCACCA TCTACACGAA CAATGTGAAT GTCGTCAGCC ATGTCATGAA 
AAAGTATTTG AGACTGCTTA CTCTGCATCT GCATGGCCCA GTCAAAATTT CAAAATTGGT 
ACCGACTGCC CCGCCGTATC AGATATTTTC AACGACACTG AAGCTTGTAC CGAATATTAT 
CGTCAAAACA CTGCTTACAT TGAAATTTAC TACGAACAAT TGAATTTCGA ATCGCTGAAA 
GAAACCGCCG GTTACACGCT CGTCAATCTT TTCTCAGATT TTGGAGGTAA TATTGGATTA 
TGGATTGGAT TTTCAGTCAT TACGTTTGCT GAATTTGCAG AACTTTTCTG CGAGATCTGC 
AAACTAATGT ATTTCAAAGG AATAGTCTAT GTACAGAAAA AGATGCAAGG AAAAGAATAC 
ACATCATCTT CATTAATGCA CATTGATTTT CTTCAACGAT CGCCTAAAAA AAGCCAACCA 
GGAGAAGATG AAGTTTCAAC AAATGAATCA ACAAAGGAGT TAATGAGCAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_501138.1
CDS	1..2334
Translation	MSPLLTWNLICVSSRWYTILCLKNKVKFWLGTRLVHEPESMESRSSPYIRPSPYAGGVHPHFEEEDDRSKLHASALYSERRTSSRKSLRSQKIDYHTTTIKSLWFDFCARTSSHGIPYVATSSFFGRYVWAALFMCMLMAFLLQTYWTMSEYLQYRTIIEMQLQFEAAAFPAATVCNLNAFKYSELTQYEEIKEGFDYWERVINARMMSDSMKPGGDILEAISVRKKRSKSRDQLLFPIDDEDLEGAVYQPVFVRCTCMNMEQCVPNRNPLEVNASICMCFEDVTRGLIWPCYPTSVWTVKKCSGCSISNTCPDPDGPNASKQIAKHNSPLPCLCQSISHHCMVHPKDEIRWWNPNNYTVYSVTEPPTTEITETEEAFGLSDLKDAGAITTQTKENLIFLVAALPRETRRNLSYTLNEFVLRCSFNSKDCSMERDFKLHVDPEYGNCYTFNFNDSVELKNSRAGPMYGLRLLLNVHQSDYMPTTEAAGVRLVVHEQDQEPFPDTFGYSAPTGFISSFGLKTKELHRLSAPWGNCSDTFRPVPYIYNEHYSPEGCHRNCFQLKVLEICGCGDPRFPLPSEEHRHCNAKSKIDRQCLSNLTSDSGGYHHLHEQCECRQPCHEKVFETAYSASAWPSQNFKIGTDCPAVSDIFNDTEACTEYYRQNTAYIEIYYEQLNFESLKETAGYTLVNLFSDFGGNIGLWIGFSVITFAEFAELFCEICKLMYFKGIVYVQKKMQGKEYTSSSLMHIDFLQRSPKKSQPGEDEVSTNESTKELMSK

Target ORF information:

RefSeq Version	NM_068737.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_068737.1

ORF Insert Sequence:

ATGTCACCTT TGCTGACGTG GAACTTGATA TGTGTCAGTT CAAGGTGGTA CACAATTTTA 
TGTTTAAAAA ATAAAGTTAA GTTCTGGTTA GGAACAAGAC TCGTCCATGA ACCAGAATCA 
ATGGAGAGCA GGTCAAGTCC ATATATTAGA CCATCTCCAT ACGCTGGAGG AGTTCATCCA 
CACTTTGAAG AGGAAGATGA CAGGTCAAAA CTTCATGCAT CAGCTCTCTA CTCGGAACGC 
CGAACGTCAT CCCGGAAATC GTTAAGATCA CAAAAAATAG ACTATCACAC AACAACAATA 
AAATCCCTTT GGTTTGATTT TTGTGCCAGA ACTTCTTCCC ATGGAATACC ATATGTTGCA 
ACATCTTCAT TTTTTGGCCG ATATGTATGG GCGGCACTCT TCATGTGCAT GTTAATGGCA 
TTCTTATTAC AAACCTATTG GACAATGTCA GAATATTTGC AGTATAGGAC AATTATTGAA 
ATGCAGTTGC AGTTTGAAGC TGCTGCATTT CCCGCTGCAA CAGTTTGCAA TTTGAATGCT 
TTCAAATATA GCGAGCTCAC GCAGTATGAA GAAATTAAAG AGGGTTTTGA CTATTGGGAA 
CGGGTGATAA ATGCTCGAAT GATGAGTGAC AGTATGAAGC CAGGAGGTGA TATTCTAGAA 
GCAATCAGTG TCAGGAAAAA GCGATCAAAA AGTCGAGATC AATTACTTTT TCCAATTGAT 
GACGAAGACT TAGAGGGTGC AGTGTATCAG CCAGTCTTTG TGAGATGCAC CTGTATGAAT 
ATGGAACAAT GTGTACCAAA TAGGAATCCT TTAGAGGTTA ACGCCTCAAT TTGTATGTGC 
TTTGAAGACG TGACACGTGG CTTAATCTGG CCATGCTACC CAACTTCCGT ATGGACTGTA 
AAAAAATGTT CTGGTTGTTC AATTTCCAAT ACTTGTCCTG ACCCGGATGG TCCTAATGCT 
TCAAAGCAAA TCGCAAAACA TAACAGTCCA CTTCCATGTC TTTGCCAATC AATTTCTCAT 
CATTGTATGG TACATCCAAA GGACGAAATC CGATGGTGGA ACCCAAACAA TTATACAGTT 
TATTCTGTAA CTGAACCACC GACCACAGAA ATAACTGAAA CCGAAGAAGC TTTTGGATTG 
AGCGACCTCA AAGATGCGGG AGCCATCACA ACTCAAACAA AAGAAAATTT AATTTTCTTA 
GTGGCTGCTT TACCCCGTGA AACAAGAAGG AACCTGTCAT ACACCCTCAA TGAGTTTGTT 
CTTCGATGTT CGTTTAATTC GAAGGATTGT AGTATGGAGC GTGACTTTAA ACTTCACGTT 
GATCCTGAAT ACGGCAATTG CTACACATTT AACTTCAATG ATTCGGTGGA GTTGAAAAAC 
AGTAGAGCTG GTCCCATGTA TGGTCTCCGT CTATTATTAA ACGTGCATCA AAGCGATTAC 
ATGCCAACAA CAGAAGCAGC AGGTGTTCGA TTAGTTGTCC ATGAACAAGA TCAAGAACCG 
TTCCCTGATA CATTTGGATA CAGTGCTCCA ACTGGATTCA TTTCTTCATT TGGTCTTAAA 
ACGAAAGAAC TACATCGTCT CAGTGCTCCA TGGGGGAATT GCAGTGACAC ATTCCGACCG 
GTTCCATACA TTTACAACGA ACACTATTCT CCTGAAGGTT GCCATCGAAA TTGTTTCCAA 
TTGAAAGTAC TTGAAATATG TGGATGTGGA GATCCAAGAT TCCCTCTACC ATCTGAAGAG 
CATAGACATT GTAATGCGAA GTCAAAAATT GATCGTCAAT GTTTATCAAA CCTAACAAGT 
GACTCTGGAG GTTACCACCA TCTACACGAA CAATGTGAAT GTCGTCAGCC ATGTCATGAA 
AAAGTATTTG AGACTGCTTA CTCTGCATCT GCATGGCCCA GTCAAAATTT CAAAATTGGT 
ACCGACTGCC CCGCCGTATC AGATATTTTC AACGACACTG AAGCTTGTAC CGAATATTAT 
CGTCAAAACA CTGCTTACAT TGAAATTTAC TACGAACAAT TGAATTTCGA ATCGCTGAAA 
GAAACCGCCG GTTACACGCT CGTCAATCTT TTCTCAGATT TTGGAGGTAA TATTGGATTA 
TGGATTGGAT TTTCAGTCAT TACGTTTGCT GAATTTGCAG AACTTTTCTG CGAGATCTGC 
AAACTAATGT ATTTCAAAGG AATAGTCTAT GTACAGAAAA AGATGCAAGG AAAAGAATAC 
ACATCATCTT CATTAATGCA CATTGATTTT CTTCAACGAT CGCCTAAAAA AAGCCAACCA 
GGAGAAGATG AAGTTTCAAC AAATGAATCA ACAAAGGAGT TAATGAGCAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-8 ( NM_068737.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_501138.1 Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.

Price & Availability↑

CloneID	OCa343690
Clone ID Related Accession (Same CDS sequence)	NM_068737.1 , NM_068737.2
Accession Version	NM_068737.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Degenerin unc-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_068737.1. COMPLETENESS: incomplete on the 5' end.

ATGTCACCTT TGCTGACGTG GAACTTGATA TGTGTCAGTT CAAGGTGGTA CACAATTTTA 
TGTTTAAAAA ATAAAGTTAA GTTCTGGTTA GGAACAAGAC TCGTCCATGA ACCAGAATCA 
ATGGAGAGCA GGTCAAGTCC ATATATTAGA CCATCTCCAT ACGCTGGAGG AGTTCATCCA 
CACTTTGAAG AGGAAGATGA CAGGTCAAAA CTTCATGCAT CAGCTCTCTA CTCGGAACGC 
CGAACGTCAT CCCGGAAATC GTTAAGATCA CAAAAAATAG ACTATCACAC AACAACAATA 
AAATCCCTTT GGTTTGATTT TTGTGCCAGA ACTTCTTCCC ATGGAATACC ATATGTTGCA 
ACATCTTCAT TTTTTGGCCG ATATGTATGG GCGGCACTCT TCATGTGCAT GTTAATGGCA 
TTCTTATTAC AAACCTATTG GACAATGTCA GAATATTTGC AGTATAGGAC AATTATTGAA 
ATGCAGTTGC AGTTTGAAGC TGCTGCATTT CCCGCTGCAA CAGTTTGCAA TTTGAATGCT 
TTCAAATATA GCGAGCTCAC GCAGTATGAA GAAATTAAAG AGGGTTTTGA CTATTGGGAA 
CGGGTGATAA ATGCTCGAAT GATGAGTGAC AGTATGAAGC CAGGAGGTGA TATTCTAGAA 
GCAATCAGTG TCAGGAAAAA GCGATCAAAA AGTCGAGATC AATTACTTTT TCCAATTGAT 
GACGAAGACT TAGAGGGTGC AGTGTATCAG CCAGTCTTTG TGAGATGCAC CTGTATGAAT 
ATGGAACAAT GTGTACCAAA TAGGAATCCT TTAGAGGTTA ACGCCTCAAT TTGTATGTGC 
TTTGAAGACG TGACACGTGG CTTAATCTGG CCATGCTACC CAACTTCCGT ATGGACTGTA 
AAAAAATGTT CTGGTTGTTC AATTTCCAAT ACTTGTCCTG ACCCGGATGG TCCTAATGCT 
TCAAAGCAAA TCGCAAAACA TAACAGTCCA CTTCCATGTC TTTGCCAATC AATTTCTCAT 
CATTGTATGG TACATCCAAA GGACGAAATC CGATGGTGGA ACCCAAACAA TTATACAGTT 
TATTCTGTAA CTGAACCACC GACCACAGAA ATAACTGAAA CCGAAGAAGC TTTTGGATTG 
AGCGACCTCA AAGATGCGGG AGCCATCACA ACTCAAACAA AAGAAAATTT AATTTTCTTA 
GTGGCTGCTT TACCCCGTGA AACAAGAAGG AACCTGTCAT ACACCCTCAA TGAGTTTGTT 
CTTCGATGTT CGTTTAATTC GAAGGATTGT AGTATGGAGC GTGACTTTAA ACTTCACGTT 
GATCCTGAAT ACGGCAATTG CTACACATTT AACTTCAATG ATTCGGTGGA GTTGAAAAAC 
AGTAGAGCTG GTCCCATGTA TGGTCTCCGT CTATTATTAA ACGTGCATCA AAGCGATTAC 
ATGCCAACAA CAGAAGCAGC AGGTGTTCGA TTAGTTGTCC ATGAACAAGA TCAAGAACCG 
TTCCCTGATA CATTTGGATA CAGTGCTCCA ACTGGATTCA TTTCTTCATT TGGTCTTAAA 
ACGAAAGAAC TACATCGTCT CAGTGCTCCA TGGGGGAATT GCAGTGACAC ATTCCGACCG 
GTTCCATACA TTTACAACGA ACACTATTCT CCTGAAGGTT GCCATCGAAA TTGTTTCCAA 
TTGAAAGTAC TTGAAATATG TGGATGTGGA GATCCAAGAT TCCCTCTACC ATCTGAAGAG 
CATAGACATT GTAATGCGAA GTCAAAAATT GATCGTCAAT GTTTATCAAA CCTAACAAGT 
GACTCTGGAG GTTACCACCA TCTACACGAA CAATGTGAAT GTCGTCAGCC ATGTCATGAA 
AAAGTATTTG AGACTGCTTA CTCTGCATCT GCATGGCCCA GTCAAAATTT CAAAATTGGT 
ACCGACTGCC CCGCCGTATC AGATATTTTC AACGACACTG AAGCTTGTAC CGAATATTAT 
CGTCAAAACA CTGCTTACAT TGAAATTTAC TACGAACAAT TGAATTTCGA ATCGCTGAAA 
GAAACCGCCG GTTACACGCT CGTCAATCTT TTCTCAGATT TTGGAGGTAA TATTGGATTA 
TGGATTGGAT TTTCAGTCAT TACGTTTGCT GAATTTGCAG AACTTTTCTG CGAGATCTGC 
AAACTAATGT ATTTCAAAGG AATAGTCTAT GTACAGAAAA AGATGCAAGG AAAAGAATAC 
ACATCATCTT CATTAATGCA CATTGATTTT CTTCAACGAT CGCCTAAAAA AAGCCAACCA 
GGAGAAGATG AAGTTTCAAC AAATGAATCA ACAAAGGAGT TAATGAGCAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_501138.1
CDS	1..2334
Translation	MSPLLTWNLICVSSRWYTILCLKNKVKFWLGTRLVHEPESMESRSSPYIRPSPYAGGVHPHFEEEDDRSKLHASALYSERRTSSRKSLRSQKIDYHTTTIKSLWFDFCARTSSHGIPYVATSSFFGRYVWAALFMCMLMAFLLQTYWTMSEYLQYRTIIEMQLQFEAAAFPAATVCNLNAFKYSELTQYEEIKEGFDYWERVINARMMSDSMKPGGDILEAISVRKKRSKSRDQLLFPIDDEDLEGAVYQPVFVRCTCMNMEQCVPNRNPLEVNASICMCFEDVTRGLIWPCYPTSVWTVKKCSGCSISNTCPDPDGPNASKQIAKHNSPLPCLCQSISHHCMVHPKDEIRWWNPNNYTVYSVTEPPTTEITETEEAFGLSDLKDAGAITTQTKENLIFLVAALPRETRRNLSYTLNEFVLRCSFNSKDCSMERDFKLHVDPEYGNCYTFNFNDSVELKNSRAGPMYGLRLLLNVHQSDYMPTTEAAGVRLVVHEQDQEPFPDTFGYSAPTGFISSFGLKTKELHRLSAPWGNCSDTFRPVPYIYNEHYSPEGCHRNCFQLKVLEICGCGDPRFPLPSEEHRHCNAKSKIDRQCLSNLTSDSGGYHHLHEQCECRQPCHEKVFETAYSASAWPSQNFKIGTDCPAVSDIFNDTEACTEYYRQNTAYIEIYYEQLNFESLKETAGYTLVNLFSDFGGNIGLWIGFSVITFAEFAELFCEICKLMYFKGIVYVQKKMQGKEYTSSSLMHIDFLQRSPKKSQPGEDEVSTNESTKELMSK

Target ORF information:

RefSeq Version	NM_068737.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_068737.2

ORF Insert Sequence:

ATGTCACCTT TGCTGACGTG GAACTTGATA TGTGTCAGTT CAAGGTGGTA CACAATTTTA 
TGTTTAAAAA ATAAAGTTAA GTTCTGGTTA GGAACAAGAC TCGTCCATGA ACCAGAATCA 
ATGGAGAGCA GGTCAAGTCC ATATATTAGA CCATCTCCAT ACGCTGGAGG AGTTCATCCA 
CACTTTGAAG AGGAAGATGA CAGGTCAAAA CTTCATGCAT CAGCTCTCTA CTCGGAACGC 
CGAACGTCAT CCCGGAAATC GTTAAGATCA CAAAAAATAG ACTATCACAC AACAACAATA 
AAATCCCTTT GGTTTGATTT TTGTGCCAGA ACTTCTTCCC ATGGAATACC ATATGTTGCA 
ACATCTTCAT TTTTTGGCCG ATATGTATGG GCGGCACTCT TCATGTGCAT GTTAATGGCA 
TTCTTATTAC AAACCTATTG GACAATGTCA GAATATTTGC AGTATAGGAC AATTATTGAA 
ATGCAGTTGC AGTTTGAAGC TGCTGCATTT CCCGCTGCAA CAGTTTGCAA TTTGAATGCT 
TTCAAATATA GCGAGCTCAC GCAGTATGAA GAAATTAAAG AGGGTTTTGA CTATTGGGAA 
CGGGTGATAA ATGCTCGAAT GATGAGTGAC AGTATGAAGC CAGGAGGTGA TATTCTAGAA 
GCAATCAGTG TCAGGAAAAA GCGATCAAAA AGTCGAGATC AATTACTTTT TCCAATTGAT 
GACGAAGACT TAGAGGGTGC AGTGTATCAG CCAGTCTTTG TGAGATGCAC CTGTATGAAT 
ATGGAACAAT GTGTACCAAA TAGGAATCCT TTAGAGGTTA ACGCCTCAAT TTGTATGTGC 
TTTGAAGACG TGACACGTGG CTTAATCTGG CCATGCTACC CAACTTCCGT ATGGACTGTA 
AAAAAATGTT CTGGTTGTTC AATTTCCAAT ACTTGTCCTG ACCCGGATGG TCCTAATGCT 
TCAAAGCAAA TCGCAAAACA TAACAGTCCA CTTCCATGTC TTTGCCAATC AATTTCTCAT 
CATTGTATGG TACATCCAAA GGACGAAATC CGATGGTGGA ACCCAAACAA TTATACAGTT 
TATTCTGTAA CTGAACCACC GACCACAGAA ATAACTGAAA CCGAAGAAGC TTTTGGATTG 
AGCGACCTCA AAGATGCGGG AGCCATCACA ACTCAAACAA AAGAAAATTT AATTTTCTTA 
GTGGCTGCTT TACCCCGTGA AACAAGAAGG AACCTGTCAT ACACCCTCAA TGAGTTTGTT 
CTTCGATGTT CGTTTAATTC GAAGGATTGT AGTATGGAGC GTGACTTTAA ACTTCACGTT 
GATCCTGAAT ACGGCAATTG CTACACATTT AACTTCAATG ATTCGGTGGA GTTGAAAAAC 
AGTAGAGCTG GTCCCATGTA TGGTCTCCGT CTATTATTAA ACGTGCATCA AAGCGATTAC 
ATGCCAACAA CAGAAGCAGC AGGTGTTCGA TTAGTTGTCC ATGAACAAGA TCAAGAACCG 
TTCCCTGATA CATTTGGATA CAGTGCTCCA ACTGGATTCA TTTCTTCATT TGGTCTTAAA 
ACGAAAGAAC TACATCGTCT CAGTGCTCCA TGGGGGAATT GCAGTGACAC ATTCCGACCG 
GTTCCATACA TTTACAACGA ACACTATTCT CCTGAAGGTT GCCATCGAAA TTGTTTCCAA 
TTGAAAGTAC TTGAAATATG TGGATGTGGA GATCCAAGAT TCCCTCTACC ATCTGAAGAG 
CATAGACATT GTAATGCGAA GTCAAAAATT GATCGTCAAT GTTTATCAAA CCTAACAAGT 
GACTCTGGAG GTTACCACCA TCTACACGAA CAATGTGAAT GTCGTCAGCC ATGTCATGAA 
AAAGTATTTG AGACTGCTTA CTCTGCATCT GCATGGCCCA GTCAAAATTT CAAAATTGGT 
ACCGACTGCC CCGCCGTATC AGATATTTTC AACGACACTG AAGCTTGTAC CGAATATTAT 
CGTCAAAACA CTGCTTACAT TGAAATTTAC TACGAACAAT TGAATTTCGA ATCGCTGAAA 
GAAACCGCCG GTTACACGCT CGTCAATCTT TTCTCAGATT TTGGAGGTAA TATTGGATTA 
TGGATTGGAT TTTCAGTCAT TACGTTTGCT GAATTTGCAG AACTTTTCTG CGAGATCTGC 
AAACTAATGT ATTTCAAAGG AATAGTCTAT GTACAGAAAA AGATGCAAGG AAAAGAATAC 
ACATCATCTT CATTAATGCA CATTGATTTT CTTCAACGAT CGCCTAAAAA AAGCCAACCA 
GGAGAAGATG AAGTTTCAAC AAATGAATCA ACAAAGGAGT TAATGAGCAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-8 ( NM_001307363.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294292.1 Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.

Price & Availability↑

CloneID	OCa353357
Clone ID Related Accession (Same CDS sequence)	NM_001307363.2 , NM_001307363.1
Accession Version	NM_001307363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Degenerin unc-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307363.1. COMPLETENESS: incomplete on the 5' end.

ATGAGCGCAA GGAGTAGTGG AAGTATGAGT ACAGCATCAC GAATGACAAA AGGATCTCCA 
GAAAGACTTG TATCGGCCAC ATCAGAGAGT AAAAGACTCG TCCATGAACC AGAATCAATG 
GAGAGCAGGT CAAGTCCATA TATTAGACCA TCTCCATACG CTGGAGGAGT TCATCCACAC 
TTTGAAGAGG AAGATGACAG GTCAAAACTT CATGCATCAG CTCTCTACTC GGAACGCCGA 
ACGTCATCCC GGAAATCGTT AAGATCACAA AAAATAGACT ATCACACAAC AACAATAAAA 
TCCCTTTGGT TTGATTTTTG TGCCAGAACT TCTTCCCATG GAATACCATA TGTTGCAACA 
TCTTCATTTT TTGGCCGATA TGTATGGGCG GCACTCTTCA TGTGCATGTT AATGGCATTC 
TTATTACAAA CCTATTGGAC AATGTCAGAA TATTTGCAGT ATAGGACAAT TATTGAAATG 
CAGTTGCAGT TTGAAGCTGC TGCATTTCCC GCTGCAACAG TTTGCAATTT GAATGCTTTC 
AAATATAGCG AGCTCACGCA GTATGAAGAA ATTAAAGAGG GTTTTGACTA TTGGGAACGG 
GTGATAAATG CTCGAATGAT GAGTGACAGT ATGAAGCCAG GAGGTGATAT TCTAGAAGCA 
ATCAGTGTCA GGAAAAAGCG ATCAAAAAGT CGAGATCAAT TACTTTTTCC AATTGATGAC 
GAAGACTTAG AGGGTGCAGT GTATCAGCCA GTCTTTGTGA GATGCACCTG TATGAATATG 
GAACAATGTG TACCAAATAG GAATCCTTTA GAGGTTAACG CCTCAATTTG TATGTGCTTT 
GAAGACGTGA CACGTGGCTT AATCTGGCCA TGCTACCCAA CTTCCGTATG GACTGTAAAA 
AAATGTTCTG GTTGTTCAAT TTCCAATACT TGTCCTGACC CGGATGGTCC TAATGCTTCA 
AAGCAAATCG CAAAACATAA CAGTCCACTT CCATGTCTTT GCCAATCAAT TTCTCATCAT 
TGTATGGTAC ATCCAAAGGA CGAAATCCGA TGGTGGAACC CAAACAATTA TACAGTTTAT 
TCTGTAACTG AACCACCGAC CACAGAAATA ACTGAAACCG AAGAAGCTTT TGGATTGAGC 
GACCTCAAAG ATGCGGGAGC CATCACAACT CAAACAAAAG AAAATTTAAT TTTCTTAGTG 
GCTGCTTTAC CCCGTGAAAC AAGAAGGAAC CTGTCATACA CCCTCAATGA GTTTGTTCTT 
CGATGTTCGT TTAATTCGAA GGATTGTAGT ATGGAGCGTG ACTTTAAACT TCACGTTGAT 
CCTGAATACG GCAATTGCTA CACATTTAAC TTCAATGATT CGGTGGAGTT GAAAAACAGT 
AGAGCTGGTC CCATGTATGG TCTCCGTCTA TTATTAAACG TGCATCAAAG CGATTACATG 
CCAACAACAG AAGCAGCAGG TGTTCGATTA GTTGTCCATG AACAAGATCA AGAACCGTTC 
CCTGATACAT TTGGATACAG TGCTCCAACT GGATTCATTT CTTCATTTGG TCTTAAAACG 
AAAGAACTAC ATCGTCTCAG TGCTCCATGG GGGAATTGCA GTGACACATT CCGACCGGTT 
CCATACATTT ACAACGAACA CTATTCTCCT GAAGGTTGCC ATCGAAATTG TTTCCAATTG 
AAAGTACTTG AAATATGTGG ATGTGGAGAT CCAAGATTCC CTCTACCATC TGAAGAGCAT 
AGACATTGTA ATGCGAAGTC AAAAATTGAT CGTCAATGTT TATCAAACCT AACAAGTGAC 
TCTGGAGGTT ACCACCATCT ACACGAACAA TGTGAATGTC GTCAGCCATG TCATGAAAAA 
GTATTTGAGA CTGCTTACTC TGCATCTGCA TGGCCCAGTC AAAATTTCAA AATTGGTACC 
GACTGCCCCG CCGTATCAGA TATTTTCAAC GACACTGAAG CTTGTACCGA ATATTATCGT 
CAAAACACTG CTTACATTGA AATTTACTAC GAACAATTGA ATTTCGAATC GCTGAAAGAA 
ACCGCCGGTT ACACGCTCGT CAATCTTTTC TCAGATTTTG GAGGTAATAT TGGATTATGG 
ATTGGATTTT CAGTCATTAC GTTTGCTGAA TTTGCAGAAC TTTTCTGCGA GATCTGCAAA 
CTAATGTATT TCAAAGGAAT AGTCTATGTA CAGAAAAAGA TGCAAGGAAA AGAATACACA 
TCATCTTCAT TAATGCACAT TGATTTTCTT CAACGATCGC CTAAAAAAAG CCAACCAGGA 
GAAGATGAAG TTTCAACAAA TGAATCAACA AAGGAGTTAA TGAGCAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294292.1
CDS	1..2331
Translation	MSARSSGSMSTASRMTKGSPERLVSATSESKRLVHEPESMESRSSPYIRPSPYAGGVHPHFEEEDDRSKLHASALYSERRTSSRKSLRSQKIDYHTTTIKSLWFDFCARTSSHGIPYVATSSFFGRYVWAALFMCMLMAFLLQTYWTMSEYLQYRTIIEMQLQFEAAAFPAATVCNLNAFKYSELTQYEEIKEGFDYWERVINARMMSDSMKPGGDILEAISVRKKRSKSRDQLLFPIDDEDLEGAVYQPVFVRCTCMNMEQCVPNRNPLEVNASICMCFEDVTRGLIWPCYPTSVWTVKKCSGCSISNTCPDPDGPNASKQIAKHNSPLPCLCQSISHHCMVHPKDEIRWWNPNNYTVYSVTEPPTTEITETEEAFGLSDLKDAGAITTQTKENLIFLVAALPRETRRNLSYTLNEFVLRCSFNSKDCSMERDFKLHVDPEYGNCYTFNFNDSVELKNSRAGPMYGLRLLLNVHQSDYMPTTEAAGVRLVVHEQDQEPFPDTFGYSAPTGFISSFGLKTKELHRLSAPWGNCSDTFRPVPYIYNEHYSPEGCHRNCFQLKVLEICGCGDPRFPLPSEEHRHCNAKSKIDRQCLSNLTSDSGGYHHLHEQCECRQPCHEKVFETAYSASAWPSQNFKIGTDCPAVSDIFNDTEACTEYYRQNTAYIEIYYEQLNFESLKETAGYTLVNLFSDFGGNIGLWIGFSVITFAEFAELFCEICKLMYFKGIVYVQKKMQGKEYTSSSLMHIDFLQRSPKKSQPGEDEVSTNESTKELMSK

Target ORF information:

RefSeq Version	NM_001307363.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307363.2

ORF Insert Sequence:

ATGAGCGCAA GGAGTAGTGG AAGTATGAGT ACAGCATCAC GAATGACAAA AGGATCTCCA 
GAAAGACTTG TATCGGCCAC ATCAGAGAGT AAAAGACTCG TCCATGAACC AGAATCAATG 
GAGAGCAGGT CAAGTCCATA TATTAGACCA TCTCCATACG CTGGAGGAGT TCATCCACAC 
TTTGAAGAGG AAGATGACAG GTCAAAACTT CATGCATCAG CTCTCTACTC GGAACGCCGA 
ACGTCATCCC GGAAATCGTT AAGATCACAA AAAATAGACT ATCACACAAC AACAATAAAA 
TCCCTTTGGT TTGATTTTTG TGCCAGAACT TCTTCCCATG GAATACCATA TGTTGCAACA 
TCTTCATTTT TTGGCCGATA TGTATGGGCG GCACTCTTCA TGTGCATGTT AATGGCATTC 
TTATTACAAA CCTATTGGAC AATGTCAGAA TATTTGCAGT ATAGGACAAT TATTGAAATG 
CAGTTGCAGT TTGAAGCTGC TGCATTTCCC GCTGCAACAG TTTGCAATTT GAATGCTTTC 
AAATATAGCG AGCTCACGCA GTATGAAGAA ATTAAAGAGG GTTTTGACTA TTGGGAACGG 
GTGATAAATG CTCGAATGAT GAGTGACAGT ATGAAGCCAG GAGGTGATAT TCTAGAAGCA 
ATCAGTGTCA GGAAAAAGCG ATCAAAAAGT CGAGATCAAT TACTTTTTCC AATTGATGAC 
GAAGACTTAG AGGGTGCAGT GTATCAGCCA GTCTTTGTGA GATGCACCTG TATGAATATG 
GAACAATGTG TACCAAATAG GAATCCTTTA GAGGTTAACG CCTCAATTTG TATGTGCTTT 
GAAGACGTGA CACGTGGCTT AATCTGGCCA TGCTACCCAA CTTCCGTATG GACTGTAAAA 
AAATGTTCTG GTTGTTCAAT TTCCAATACT TGTCCTGACC CGGATGGTCC TAATGCTTCA 
AAGCAAATCG CAAAACATAA CAGTCCACTT CCATGTCTTT GCCAATCAAT TTCTCATCAT 
TGTATGGTAC ATCCAAAGGA CGAAATCCGA TGGTGGAACC CAAACAATTA TACAGTTTAT 
TCTGTAACTG AACCACCGAC CACAGAAATA ACTGAAACCG AAGAAGCTTT TGGATTGAGC 
GACCTCAAAG ATGCGGGAGC CATCACAACT CAAACAAAAG AAAATTTAAT TTTCTTAGTG 
GCTGCTTTAC CCCGTGAAAC AAGAAGGAAC CTGTCATACA CCCTCAATGA GTTTGTTCTT 
CGATGTTCGT TTAATTCGAA GGATTGTAGT ATGGAGCGTG ACTTTAAACT TCACGTTGAT 
CCTGAATACG GCAATTGCTA CACATTTAAC TTCAATGATT CGGTGGAGTT GAAAAACAGT 
AGAGCTGGTC CCATGTATGG TCTCCGTCTA TTATTAAACG TGCATCAAAG CGATTACATG 
CCAACAACAG AAGCAGCAGG TGTTCGATTA GTTGTCCATG AACAAGATCA AGAACCGTTC 
CCTGATACAT TTGGATACAG TGCTCCAACT GGATTCATTT CTTCATTTGG TCTTAAAACG 
AAAGAACTAC ATCGTCTCAG TGCTCCATGG GGGAATTGCA GTGACACATT CCGACCGGTT 
CCATACATTT ACAACGAACA CTATTCTCCT GAAGGTTGCC ATCGAAATTG TTTCCAATTG 
AAAGTACTTG AAATATGTGG ATGTGGAGAT CCAAGATTCC CTCTACCATC TGAAGAGCAT 
AGACATTGTA ATGCGAAGTC AAAAATTGAT CGTCAATGTT TATCAAACCT AACAAGTGAC 
TCTGGAGGTT ACCACCATCT ACACGAACAA TGTGAATGTC GTCAGCCATG TCATGAAAAA 
GTATTTGAGA CTGCTTACTC TGCATCTGCA TGGCCCAGTC AAAATTTCAA AATTGGTACC 
GACTGCCCCG CCGTATCAGA TATTTTCAAC GACACTGAAG CTTGTACCGA ATATTATCGT 
CAAAACACTG CTTACATTGA AATTTACTAC GAACAATTGA ATTTCGAATC GCTGAAAGAA 
ACCGCCGGTT ACACGCTCGT CAATCTTTTC TCAGATTTTG GAGGTAATAT TGGATTATGG 
ATTGGATTTT CAGTCATTAC GTTTGCTGAA TTTGCAGAAC TTTTCTGCGA GATCTGCAAA 
CTAATGTATT TCAAAGGAAT AGTCTATGTA CAGAAAAAGA TGCAAGGAAA AGAATACACA 
TCATCTTCAT TAATGCACAT TGATTTTCTT CAACGATCGC CTAAAAAAAG CCAACCAGGA 
GAAGATGAAG TTTCAACAAA TGAATCAACA AAGGAGTTAA TGAGCAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-8 ( NM_001307363.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294292.1 Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.

Price & Availability↑

CloneID	OCa353357
Clone ID Related Accession (Same CDS sequence)	NM_001307363.2 , NM_001307363.1
Accession Version	NM_001307363.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Degenerin unc-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGAGCGCAA GGAGTAGTGG AAGTATGAGT ACAGCATCAC GAATGACAAA AGGATCTCCA 
GAAAGACTTG TATCGGCCAC ATCAGAGAGT AAAAGACTCG TCCATGAACC AGAATCAATG 
GAGAGCAGGT CAAGTCCATA TATTAGACCA TCTCCATACG CTGGAGGAGT TCATCCACAC 
TTTGAAGAGG AAGATGACAG GTCAAAACTT CATGCATCAG CTCTCTACTC GGAACGCCGA 
ACGTCATCCC GGAAATCGTT AAGATCACAA AAAATAGACT ATCACACAAC AACAATAAAA 
TCCCTTTGGT TTGATTTTTG TGCCAGAACT TCTTCCCATG GAATACCATA TGTTGCAACA 
TCTTCATTTT TTGGCCGATA TGTATGGGCG GCACTCTTCA TGTGCATGTT AATGGCATTC 
TTATTACAAA CCTATTGGAC AATGTCAGAA TATTTGCAGT ATAGGACAAT TATTGAAATG 
CAGTTGCAGT TTGAAGCTGC TGCATTTCCC GCTGCAACAG TTTGCAATTT GAATGCTTTC 
AAATATAGCG AGCTCACGCA GTATGAAGAA ATTAAAGAGG GTTTTGACTA TTGGGAACGG 
GTGATAAATG CTCGAATGAT GAGTGACAGT ATGAAGCCAG GAGGTGATAT TCTAGAAGCA 
ATCAGTGTCA GGAAAAAGCG ATCAAAAAGT CGAGATCAAT TACTTTTTCC AATTGATGAC 
GAAGACTTAG AGGGTGCAGT GTATCAGCCA GTCTTTGTGA GATGCACCTG TATGAATATG 
GAACAATGTG TACCAAATAG GAATCCTTTA GAGGTTAACG CCTCAATTTG TATGTGCTTT 
GAAGACGTGA CACGTGGCTT AATCTGGCCA TGCTACCCAA CTTCCGTATG GACTGTAAAA 
AAATGTTCTG GTTGTTCAAT TTCCAATACT TGTCCTGACC CGGATGGTCC TAATGCTTCA 
AAGCAAATCG CAAAACATAA CAGTCCACTT CCATGTCTTT GCCAATCAAT TTCTCATCAT 
TGTATGGTAC ATCCAAAGGA CGAAATCCGA TGGTGGAACC CAAACAATTA TACAGTTTAT 
TCTGTAACTG AACCACCGAC CACAGAAATA ACTGAAACCG AAGAAGCTTT TGGATTGAGC 
GACCTCAAAG ATGCGGGAGC CATCACAACT CAAACAAAAG AAAATTTAAT TTTCTTAGTG 
GCTGCTTTAC CCCGTGAAAC AAGAAGGAAC CTGTCATACA CCCTCAATGA GTTTGTTCTT 
CGATGTTCGT TTAATTCGAA GGATTGTAGT ATGGAGCGTG ACTTTAAACT TCACGTTGAT 
CCTGAATACG GCAATTGCTA CACATTTAAC TTCAATGATT CGGTGGAGTT GAAAAACAGT 
AGAGCTGGTC CCATGTATGG TCTCCGTCTA TTATTAAACG TGCATCAAAG CGATTACATG 
CCAACAACAG AAGCAGCAGG TGTTCGATTA GTTGTCCATG AACAAGATCA AGAACCGTTC 
CCTGATACAT TTGGATACAG TGCTCCAACT GGATTCATTT CTTCATTTGG TCTTAAAACG 
AAAGAACTAC ATCGTCTCAG TGCTCCATGG GGGAATTGCA GTGACACATT CCGACCGGTT 
CCATACATTT ACAACGAACA CTATTCTCCT GAAGGTTGCC ATCGAAATTG TTTCCAATTG 
AAAGTACTTG AAATATGTGG ATGTGGAGAT CCAAGATTCC CTCTACCATC TGAAGAGCAT 
AGACATTGTA ATGCGAAGTC AAAAATTGAT CGTCAATGTT TATCAAACCT AACAAGTGAC 
TCTGGAGGTT ACCACCATCT ACACGAACAA TGTGAATGTC GTCAGCCATG TCATGAAAAA 
GTATTTGAGA CTGCTTACTC TGCATCTGCA TGGCCCAGTC AAAATTTCAA AATTGGTACC 
GACTGCCCCG CCGTATCAGA TATTTTCAAC GACACTGAAG CTTGTACCGA ATATTATCGT 
CAAAACACTG CTTACATTGA AATTTACTAC GAACAATTGA ATTTCGAATC GCTGAAAGAA 
ACCGCCGGTT ACACGCTCGT CAATCTTTTC TCAGATTTTG GAGGTAATAT TGGATTATGG 
ATTGGATTTT CAGTCATTAC GTTTGCTGAA TTTGCAGAAC TTTTCTGCGA GATCTGCAAA 
CTAATGTATT TCAAAGGAAT AGTCTATGTA CAGAAAAAGA TGCAAGGAAA AGAATACACA 
TCATCTTCAT TAATGCACAT TGATTTTCTT CAACGATCGC CTAAAAAAAG CCAACCAGGA 
GAAGATGAAG TTTCAACAAA TGAATCAACA AAGGAGTTAA TGAGCAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294292.1
CDS	1..2331
Translation	MSARSSGSMSTASRMTKGSPERLVSATSESKRLVHEPESMESRSSPYIRPSPYAGGVHPHFEEEDDRSKLHASALYSERRTSSRKSLRSQKIDYHTTTIKSLWFDFCARTSSHGIPYVATSSFFGRYVWAALFMCMLMAFLLQTYWTMSEYLQYRTIIEMQLQFEAAAFPAATVCNLNAFKYSELTQYEEIKEGFDYWERVINARMMSDSMKPGGDILEAISVRKKRSKSRDQLLFPIDDEDLEGAVYQPVFVRCTCMNMEQCVPNRNPLEVNASICMCFEDVTRGLIWPCYPTSVWTVKKCSGCSISNTCPDPDGPNASKQIAKHNSPLPCLCQSISHHCMVHPKDEIRWWNPNNYTVYSVTEPPTTEITETEEAFGLSDLKDAGAITTQTKENLIFLVAALPRETRRNLSYTLNEFVLRCSFNSKDCSMERDFKLHVDPEYGNCYTFNFNDSVELKNSRAGPMYGLRLLLNVHQSDYMPTTEAAGVRLVVHEQDQEPFPDTFGYSAPTGFISSFGLKTKELHRLSAPWGNCSDTFRPVPYIYNEHYSPEGCHRNCFQLKVLEICGCGDPRFPLPSEEHRHCNAKSKIDRQCLSNLTSDSGGYHHLHEQCECRQPCHEKVFETAYSASAWPSQNFKIGTDCPAVSDIFNDTEACTEYYRQNTAYIEIYYEQLNFESLKETAGYTLVNLFSDFGGNIGLWIGFSVITFAEFAELFCEICKLMYFKGIVYVQKKMQGKEYTSSSLMHIDFLQRSPKKSQPGEDEVSTNESTKELMSK

Target ORF information:

RefSeq Version	NM_001307363.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307363.1

ORF Insert Sequence:

ATGAGCGCAA GGAGTAGTGG AAGTATGAGT ACAGCATCAC GAATGACAAA AGGATCTCCA 
GAAAGACTTG TATCGGCCAC ATCAGAGAGT AAAAGACTCG TCCATGAACC AGAATCAATG 
GAGAGCAGGT CAAGTCCATA TATTAGACCA TCTCCATACG CTGGAGGAGT TCATCCACAC 
TTTGAAGAGG AAGATGACAG GTCAAAACTT CATGCATCAG CTCTCTACTC GGAACGCCGA 
ACGTCATCCC GGAAATCGTT AAGATCACAA AAAATAGACT ATCACACAAC AACAATAAAA 
TCCCTTTGGT TTGATTTTTG TGCCAGAACT TCTTCCCATG GAATACCATA TGTTGCAACA 
TCTTCATTTT TTGGCCGATA TGTATGGGCG GCACTCTTCA TGTGCATGTT AATGGCATTC 
TTATTACAAA CCTATTGGAC AATGTCAGAA TATTTGCAGT ATAGGACAAT TATTGAAATG 
CAGTTGCAGT TTGAAGCTGC TGCATTTCCC GCTGCAACAG TTTGCAATTT GAATGCTTTC 
AAATATAGCG AGCTCACGCA GTATGAAGAA ATTAAAGAGG GTTTTGACTA TTGGGAACGG 
GTGATAAATG CTCGAATGAT GAGTGACAGT ATGAAGCCAG GAGGTGATAT TCTAGAAGCA 
ATCAGTGTCA GGAAAAAGCG ATCAAAAAGT CGAGATCAAT TACTTTTTCC AATTGATGAC 
GAAGACTTAG AGGGTGCAGT GTATCAGCCA GTCTTTGTGA GATGCACCTG TATGAATATG 
GAACAATGTG TACCAAATAG GAATCCTTTA GAGGTTAACG CCTCAATTTG TATGTGCTTT 
GAAGACGTGA CACGTGGCTT AATCTGGCCA TGCTACCCAA CTTCCGTATG GACTGTAAAA 
AAATGTTCTG GTTGTTCAAT TTCCAATACT TGTCCTGACC CGGATGGTCC TAATGCTTCA 
AAGCAAATCG CAAAACATAA CAGTCCACTT CCATGTCTTT GCCAATCAAT TTCTCATCAT 
TGTATGGTAC ATCCAAAGGA CGAAATCCGA TGGTGGAACC CAAACAATTA TACAGTTTAT 
TCTGTAACTG AACCACCGAC CACAGAAATA ACTGAAACCG AAGAAGCTTT TGGATTGAGC 
GACCTCAAAG ATGCGGGAGC CATCACAACT CAAACAAAAG AAAATTTAAT TTTCTTAGTG 
GCTGCTTTAC CCCGTGAAAC AAGAAGGAAC CTGTCATACA CCCTCAATGA GTTTGTTCTT 
CGATGTTCGT TTAATTCGAA GGATTGTAGT ATGGAGCGTG ACTTTAAACT TCACGTTGAT 
CCTGAATACG GCAATTGCTA CACATTTAAC TTCAATGATT CGGTGGAGTT GAAAAACAGT 
AGAGCTGGTC CCATGTATGG TCTCCGTCTA TTATTAAACG TGCATCAAAG CGATTACATG 
CCAACAACAG AAGCAGCAGG TGTTCGATTA GTTGTCCATG AACAAGATCA AGAACCGTTC 
CCTGATACAT TTGGATACAG TGCTCCAACT GGATTCATTT CTTCATTTGG TCTTAAAACG 
AAAGAACTAC ATCGTCTCAG TGCTCCATGG GGGAATTGCA GTGACACATT CCGACCGGTT 
CCATACATTT ACAACGAACA CTATTCTCCT GAAGGTTGCC ATCGAAATTG TTTCCAATTG 
AAAGTACTTG AAATATGTGG ATGTGGAGAT CCAAGATTCC CTCTACCATC TGAAGAGCAT 
AGACATTGTA ATGCGAAGTC AAAAATTGAT CGTCAATGTT TATCAAACCT AACAAGTGAC 
TCTGGAGGTT ACCACCATCT ACACGAACAA TGTGAATGTC GTCAGCCATG TCATGAAAAA 
GTATTTGAGA CTGCTTACTC TGCATCTGCA TGGCCCAGTC AAAATTTCAA AATTGGTACC 
GACTGCCCCG CCGTATCAGA TATTTTCAAC GACACTGAAG CTTGTACCGA ATATTATCGT 
CAAAACACTG CTTACATTGA AATTTACTAC GAACAATTGA ATTTCGAATC GCTGAAAGAA 
ACCGCCGGTT ACACGCTCGT CAATCTTTTC TCAGATTTTG GAGGTAATAT TGGATTATGG 
ATTGGATTTT CAGTCATTAC GTTTGCTGAA TTTGCAGAAC TTTTCTGCGA GATCTGCAAA 
CTAATGTATT TCAAAGGAAT AGTCTATGTA CAGAAAAAGA TGCAAGGAAA AGAATACACA 
TCATCTTCAT TAATGCACAT TGATTTTCTT CAACGATCGC CTAAAAAAAG CCAACCAGGA 
GAAGATGAAG TTTCAACAAA TGAATCAACA AAGGAGTTAA TGAGCAAATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-8 ( NM_001307364.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294293.1 Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.

Price & Availability↑

CloneID	OCa353358
Clone ID Related Accession (Same CDS sequence)	NM_001307364.1 , NM_001307364.2
Accession Version	NM_001307364.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Degenerin unc-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGATTCCAA AATATACATT TCCACGTCGC AAGGATGCAA TTCGGCACAA AATTCGAAAA 
AATGTCACCT TTGCTGACGT GGAACTTGAT ATGTGTCAGA GACTCGTCCA TGAACCAGAA 
TCAATGGAGA GCAGGTCAAG TCCATATATT AGACCATCTC CATACGCTGG AGGAGTTCAT 
CCACACTTTG AAGAGGAAGA TGACAGGTCA AAACTTCATG CATCAGCTCT CTACTCGGAA 
CGCCGAACGT CATCCCGGAA ATCGTTAAGA TCACAAAAAA TAGACTATCA CACAACAACA 
ATAAAATCCC TTTGGTTTGA TTTTTGTGCC AGAACTTCTT CCCATGGAAT ACCATATGTT 
GCAACATCTT CATTTTTTGG CCGATATGTA TGGGCGGCAC TCTTCATGTG CATGTTAATG 
GCATTCTTAT TACAAACCTA TTGGACAATG TCAGAATATT TGCAGTATAG GACAATTATT 
GAAATGCAGT TGCAGTTTGA AGCTGCTGCA TTTCCCGCTG CAACAGTTTG CAATTTGAAT 
GCTTTCAAAT ATAGCGAGCT CACGCAGTAT GAAGAAATTA AAGAGGGTTT TGACTATTGG 
GAACGGGTGA TAAATGCTCG AATGATGAGT GACAGTATGA AGCCAGGAGG TGATATTCTA 
GAAGCAATCA GTGTCAGGAA AAAGCGATCA AAAAGTCGAG ATCAATTACT TTTTCCAATT 
GATGACGAAG ACTTAGAGGG TGCAGTGTAT CAGCCAGTCT TTGTGAGATG CACCTGTATG 
AATATGGAAC AATGTGTACC AAATAGGAAT CCTTTAGAGG TTAACGCCTC AATTTGTATG 
TGCTTTGAAG ACGTGACACG TGGCTTAATC TGGCCATGCT ACCCAACTTC CGTATGGACT 
GTAAAAAAAT GTTCTGGTTG TTCAATTTCC AATACTTGTC CTGACCCGGA TGGTCCTAAT 
GCTTCAAAGC AAATCGCAAA ACATAACAGT CCACTTCCAT GTCTTTGCCA ATCAATTTCT 
CATCATTGTA TGGTACATCC AAAGGACGAA ATCCGATGGT GGAACCCAAA CAATTATACA 
GTTTATTCTG TAACTGAACC ACCGACCACA GAAATAACTG AAACCGAAGA AGCTTTTGGA 
TTGAGCGACC TCAAAGATGC GGGAGCCATC ACAACTCAAA CAAAAGAAAA TTTAATTTTC 
TTAGTGGCTG CTTTACCCCG TGAAACAAGA AGGAACCTGT CATACACCCT CAATGAGTTT 
GTTCTTCGAT GTTCGTTTAA TTCGAAGGAT TGTAGTATGG AGCGTGACTT TAAACTTCAC 
GTTGATCCTG AATACGGCAA TTGCTACACA TTTAACTTCA ATGATTCGGT GGAGTTGAAA 
AACAGTAGAG CTGGTCCCAT GTATGGTCTC CGTCTATTAT TAAACGTGCA TCAAAGCGAT 
TACATGCCAA CAACAGAAGC AGCAGGTGTT CGATTAGTTG TCCATGAACA AGATCAAGAA 
CCGTTCCCTG ATACATTTGG ATACAGTGCT CCAACTGGAT TCATTTCTTC ATTTGGTCTT 
AAAACGAAAG AACTACATCG TCTCAGTGCT CCATGGGGGA ATTGCAGTGA CACATTCCGA 
CCGGTTCCAT ACATTTACAA CGAACACTAT TCTCCTGAAG GTTGCCATCG AAATTGTTTC 
CAATTGAAAG TACTTGAAAT ATGTGGATGT GGAGATCCAA GATTCCCTCT ACCATCTGAA 
GAGCATAGAC ATTGTAATGC GAAGTCAAAA ATTGATCGTC AATGTTTATC AAACCTAACA 
AGTGACTCTG GAGGTTACCA CCATCTACAC GAACAATGTG AATGTCGTCA GCCATGTCAT 
GAAAAAGTAT TTGAGACTGC TTACTCTGCA TCTGCATGGC CCAGTCAAAA TTTCAAAATT 
GGTACCGACT GCCCCGCCGT ATCAGATATT TTCAACGACA CTGAAGCTTG TACCGAATAT 
TATCGTCAAA ACACTGCTTA CATTGAAATT TACTACGAAC AATTGAATTT CGAATCGCTG 
AAAGAAACCG CCGGTTACAC GCTCGTCAAT CTTTTCTCAG ATTTTGGAGG TAATATTGGA 
TTATGGATTG GATTTTCAGT CATTACGTTT GCTGAATTTG CAGAACTTTT CTGCGAGATC 
TGCAAACTAA TGTATTTCAA AGGAATAGTC TATGTACAGA AAAAGATGCA AGGAAAAGAA 
TACACATCAT CTTCATTAAT GCACATTGAT TTTCTTCAAC GATCGCCTAA AAAAAGCCAA 
CCAGGAGAAG ATGAAGTTTC AACAAATGAA TCAACAAAGG AGTTAATGAG CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294293.1
CDS	1..2337
Translation	MIPKYTFPRRKDAIRHKIRKNVTFADVELDMCQRLVHEPESMESRSSPYIRPSPYAGGVHPHFEEEDDRSKLHASALYSERRTSSRKSLRSQKIDYHTTTIKSLWFDFCARTSSHGIPYVATSSFFGRYVWAALFMCMLMAFLLQTYWTMSEYLQYRTIIEMQLQFEAAAFPAATVCNLNAFKYSELTQYEEIKEGFDYWERVINARMMSDSMKPGGDILEAISVRKKRSKSRDQLLFPIDDEDLEGAVYQPVFVRCTCMNMEQCVPNRNPLEVNASICMCFEDVTRGLIWPCYPTSVWTVKKCSGCSISNTCPDPDGPNASKQIAKHNSPLPCLCQSISHHCMVHPKDEIRWWNPNNYTVYSVTEPPTTEITETEEAFGLSDLKDAGAITTQTKENLIFLVAALPRETRRNLSYTLNEFVLRCSFNSKDCSMERDFKLHVDPEYGNCYTFNFNDSVELKNSRAGPMYGLRLLLNVHQSDYMPTTEAAGVRLVVHEQDQEPFPDTFGYSAPTGFISSFGLKTKELHRLSAPWGNCSDTFRPVPYIYNEHYSPEGCHRNCFQLKVLEICGCGDPRFPLPSEEHRHCNAKSKIDRQCLSNLTSDSGGYHHLHEQCECRQPCHEKVFETAYSASAWPSQNFKIGTDCPAVSDIFNDTEACTEYYRQNTAYIEIYYEQLNFESLKETAGYTLVNLFSDFGGNIGLWIGFSVITFAEFAELFCEICKLMYFKGIVYVQKKMQGKEYTSSSLMHIDFLQRSPKKSQPGEDEVSTNESTKELMSK

Target ORF information:

RefSeq Version	NM_001307364.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307364.1

ORF Insert Sequence:

ATGATTCCAA AATATACATT TCCACGTCGC AAGGATGCAA TTCGGCACAA AATTCGAAAA 
AATGTCACCT TTGCTGACGT GGAACTTGAT ATGTGTCAGA GACTCGTCCA TGAACCAGAA 
TCAATGGAGA GCAGGTCAAG TCCATATATT AGACCATCTC CATACGCTGG AGGAGTTCAT 
CCACACTTTG AAGAGGAAGA TGACAGGTCA AAACTTCATG CATCAGCTCT CTACTCGGAA 
CGCCGAACGT CATCCCGGAA ATCGTTAAGA TCACAAAAAA TAGACTATCA CACAACAACA 
ATAAAATCCC TTTGGTTTGA TTTTTGTGCC AGAACTTCTT CCCATGGAAT ACCATATGTT 
GCAACATCTT CATTTTTTGG CCGATATGTA TGGGCGGCAC TCTTCATGTG CATGTTAATG 
GCATTCTTAT TACAAACCTA TTGGACAATG TCAGAATATT TGCAGTATAG GACAATTATT 
GAAATGCAGT TGCAGTTTGA AGCTGCTGCA TTTCCCGCTG CAACAGTTTG CAATTTGAAT 
GCTTTCAAAT ATAGCGAGCT CACGCAGTAT GAAGAAATTA AAGAGGGTTT TGACTATTGG 
GAACGGGTGA TAAATGCTCG AATGATGAGT GACAGTATGA AGCCAGGAGG TGATATTCTA 
GAAGCAATCA GTGTCAGGAA AAAGCGATCA AAAAGTCGAG ATCAATTACT TTTTCCAATT 
GATGACGAAG ACTTAGAGGG TGCAGTGTAT CAGCCAGTCT TTGTGAGATG CACCTGTATG 
AATATGGAAC AATGTGTACC AAATAGGAAT CCTTTAGAGG TTAACGCCTC AATTTGTATG 
TGCTTTGAAG ACGTGACACG TGGCTTAATC TGGCCATGCT ACCCAACTTC CGTATGGACT 
GTAAAAAAAT GTTCTGGTTG TTCAATTTCC AATACTTGTC CTGACCCGGA TGGTCCTAAT 
GCTTCAAAGC AAATCGCAAA ACATAACAGT CCACTTCCAT GTCTTTGCCA ATCAATTTCT 
CATCATTGTA TGGTACATCC AAAGGACGAA ATCCGATGGT GGAACCCAAA CAATTATACA 
GTTTATTCTG TAACTGAACC ACCGACCACA GAAATAACTG AAACCGAAGA AGCTTTTGGA 
TTGAGCGACC TCAAAGATGC GGGAGCCATC ACAACTCAAA CAAAAGAAAA TTTAATTTTC 
TTAGTGGCTG CTTTACCCCG TGAAACAAGA AGGAACCTGT CATACACCCT CAATGAGTTT 
GTTCTTCGAT GTTCGTTTAA TTCGAAGGAT TGTAGTATGG AGCGTGACTT TAAACTTCAC 
GTTGATCCTG AATACGGCAA TTGCTACACA TTTAACTTCA ATGATTCGGT GGAGTTGAAA 
AACAGTAGAG CTGGTCCCAT GTATGGTCTC CGTCTATTAT TAAACGTGCA TCAAAGCGAT 
TACATGCCAA CAACAGAAGC AGCAGGTGTT CGATTAGTTG TCCATGAACA AGATCAAGAA 
CCGTTCCCTG ATACATTTGG ATACAGTGCT CCAACTGGAT TCATTTCTTC ATTTGGTCTT 
AAAACGAAAG AACTACATCG TCTCAGTGCT CCATGGGGGA ATTGCAGTGA CACATTCCGA 
CCGGTTCCAT ACATTTACAA CGAACACTAT TCTCCTGAAG GTTGCCATCG AAATTGTTTC 
CAATTGAAAG TACTTGAAAT ATGTGGATGT GGAGATCCAA GATTCCCTCT ACCATCTGAA 
GAGCATAGAC ATTGTAATGC GAAGTCAAAA ATTGATCGTC AATGTTTATC AAACCTAACA 
AGTGACTCTG GAGGTTACCA CCATCTACAC GAACAATGTG AATGTCGTCA GCCATGTCAT 
GAAAAAGTAT TTGAGACTGC TTACTCTGCA TCTGCATGGC CCAGTCAAAA TTTCAAAATT 
GGTACCGACT GCCCCGCCGT ATCAGATATT TTCAACGACA CTGAAGCTTG TACCGAATAT 
TATCGTCAAA ACACTGCTTA CATTGAAATT TACTACGAAC AATTGAATTT CGAATCGCTG 
AAAGAAACCG CCGGTTACAC GCTCGTCAAT CTTTTCTCAG ATTTTGGAGG TAATATTGGA 
TTATGGATTG GATTTTCAGT CATTACGTTT GCTGAATTTG CAGAACTTTT CTGCGAGATC 
TGCAAACTAA TGTATTTCAA AGGAATAGTC TATGTACAGA AAAAGATGCA AGGAAAAGAA 
TACACATCAT CTTCATTAAT GCACATTGAT TTTCTTCAAC GATCGCCTAA AAAAAGCCAA 
CCAGGAGAAG ATGAAGTTTC AACAAATGAA TCAACAAAGG AGTTAATGAG CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-8 ( NM_001307364.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294293.1 Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.

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CloneID	OCa353358
Clone ID Related Accession (Same CDS sequence)	NM_001307364.1 , NM_001307364.2
Accession Version	NM_001307364.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Degenerin unc-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307364.1. COMPLETENESS: incomplete on the 5' end.

ATGATTCCAA AATATACATT TCCACGTCGC AAGGATGCAA TTCGGCACAA AATTCGAAAA 
AATGTCACCT TTGCTGACGT GGAACTTGAT ATGTGTCAGA GACTCGTCCA TGAACCAGAA 
TCAATGGAGA GCAGGTCAAG TCCATATATT AGACCATCTC CATACGCTGG AGGAGTTCAT 
CCACACTTTG AAGAGGAAGA TGACAGGTCA AAACTTCATG CATCAGCTCT CTACTCGGAA 
CGCCGAACGT CATCCCGGAA ATCGTTAAGA TCACAAAAAA TAGACTATCA CACAACAACA 
ATAAAATCCC TTTGGTTTGA TTTTTGTGCC AGAACTTCTT CCCATGGAAT ACCATATGTT 
GCAACATCTT CATTTTTTGG CCGATATGTA TGGGCGGCAC TCTTCATGTG CATGTTAATG 
GCATTCTTAT TACAAACCTA TTGGACAATG TCAGAATATT TGCAGTATAG GACAATTATT 
GAAATGCAGT TGCAGTTTGA AGCTGCTGCA TTTCCCGCTG CAACAGTTTG CAATTTGAAT 
GCTTTCAAAT ATAGCGAGCT CACGCAGTAT GAAGAAATTA AAGAGGGTTT TGACTATTGG 
GAACGGGTGA TAAATGCTCG AATGATGAGT GACAGTATGA AGCCAGGAGG TGATATTCTA 
GAAGCAATCA GTGTCAGGAA AAAGCGATCA AAAAGTCGAG ATCAATTACT TTTTCCAATT 
GATGACGAAG ACTTAGAGGG TGCAGTGTAT CAGCCAGTCT TTGTGAGATG CACCTGTATG 
AATATGGAAC AATGTGTACC AAATAGGAAT CCTTTAGAGG TTAACGCCTC AATTTGTATG 
TGCTTTGAAG ACGTGACACG TGGCTTAATC TGGCCATGCT ACCCAACTTC CGTATGGACT 
GTAAAAAAAT GTTCTGGTTG TTCAATTTCC AATACTTGTC CTGACCCGGA TGGTCCTAAT 
GCTTCAAAGC AAATCGCAAA ACATAACAGT CCACTTCCAT GTCTTTGCCA ATCAATTTCT 
CATCATTGTA TGGTACATCC AAAGGACGAA ATCCGATGGT GGAACCCAAA CAATTATACA 
GTTTATTCTG TAACTGAACC ACCGACCACA GAAATAACTG AAACCGAAGA AGCTTTTGGA 
TTGAGCGACC TCAAAGATGC GGGAGCCATC ACAACTCAAA CAAAAGAAAA TTTAATTTTC 
TTAGTGGCTG CTTTACCCCG TGAAACAAGA AGGAACCTGT CATACACCCT CAATGAGTTT 
GTTCTTCGAT GTTCGTTTAA TTCGAAGGAT TGTAGTATGG AGCGTGACTT TAAACTTCAC 
GTTGATCCTG AATACGGCAA TTGCTACACA TTTAACTTCA ATGATTCGGT GGAGTTGAAA 
AACAGTAGAG CTGGTCCCAT GTATGGTCTC CGTCTATTAT TAAACGTGCA TCAAAGCGAT 
TACATGCCAA CAACAGAAGC AGCAGGTGTT CGATTAGTTG TCCATGAACA AGATCAAGAA 
CCGTTCCCTG ATACATTTGG ATACAGTGCT CCAACTGGAT TCATTTCTTC ATTTGGTCTT 
AAAACGAAAG AACTACATCG TCTCAGTGCT CCATGGGGGA ATTGCAGTGA CACATTCCGA 
CCGGTTCCAT ACATTTACAA CGAACACTAT TCTCCTGAAG GTTGCCATCG AAATTGTTTC 
CAATTGAAAG TACTTGAAAT ATGTGGATGT GGAGATCCAA GATTCCCTCT ACCATCTGAA 
GAGCATAGAC ATTGTAATGC GAAGTCAAAA ATTGATCGTC AATGTTTATC AAACCTAACA 
AGTGACTCTG GAGGTTACCA CCATCTACAC GAACAATGTG AATGTCGTCA GCCATGTCAT 
GAAAAAGTAT TTGAGACTGC TTACTCTGCA TCTGCATGGC CCAGTCAAAA TTTCAAAATT 
GGTACCGACT GCCCCGCCGT ATCAGATATT TTCAACGACA CTGAAGCTTG TACCGAATAT 
TATCGTCAAA ACACTGCTTA CATTGAAATT TACTACGAAC AATTGAATTT CGAATCGCTG 
AAAGAAACCG CCGGTTACAC GCTCGTCAAT CTTTTCTCAG ATTTTGGAGG TAATATTGGA 
TTATGGATTG GATTTTCAGT CATTACGTTT GCTGAATTTG CAGAACTTTT CTGCGAGATC 
TGCAAACTAA TGTATTTCAA AGGAATAGTC TATGTACAGA AAAAGATGCA AGGAAAAGAA 
TACACATCAT CTTCATTAAT GCACATTGAT TTTCTTCAAC GATCGCCTAA AAAAAGCCAA 
CCAGGAGAAG ATGAAGTTTC AACAAATGAA TCAACAAAGG AGTTAATGAG CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294293.1
CDS	1..2337
Translation	MIPKYTFPRRKDAIRHKIRKNVTFADVELDMCQRLVHEPESMESRSSPYIRPSPYAGGVHPHFEEEDDRSKLHASALYSERRTSSRKSLRSQKIDYHTTTIKSLWFDFCARTSSHGIPYVATSSFFGRYVWAALFMCMLMAFLLQTYWTMSEYLQYRTIIEMQLQFEAAAFPAATVCNLNAFKYSELTQYEEIKEGFDYWERVINARMMSDSMKPGGDILEAISVRKKRSKSRDQLLFPIDDEDLEGAVYQPVFVRCTCMNMEQCVPNRNPLEVNASICMCFEDVTRGLIWPCYPTSVWTVKKCSGCSISNTCPDPDGPNASKQIAKHNSPLPCLCQSISHHCMVHPKDEIRWWNPNNYTVYSVTEPPTTEITETEEAFGLSDLKDAGAITTQTKENLIFLVAALPRETRRNLSYTLNEFVLRCSFNSKDCSMERDFKLHVDPEYGNCYTFNFNDSVELKNSRAGPMYGLRLLLNVHQSDYMPTTEAAGVRLVVHEQDQEPFPDTFGYSAPTGFISSFGLKTKELHRLSAPWGNCSDTFRPVPYIYNEHYSPEGCHRNCFQLKVLEICGCGDPRFPLPSEEHRHCNAKSKIDRQCLSNLTSDSGGYHHLHEQCECRQPCHEKVFETAYSASAWPSQNFKIGTDCPAVSDIFNDTEACTEYYRQNTAYIEIYYEQLNFESLKETAGYTLVNLFSDFGGNIGLWIGFSVITFAEFAELFCEICKLMYFKGIVYVQKKMQGKEYTSSSLMHIDFLQRSPKKSQPGEDEVSTNESTKELMSK

Target ORF information:

RefSeq Version	NM_001307364.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307364.2

ORF Insert Sequence:

ATGATTCCAA AATATACATT TCCACGTCGC AAGGATGCAA TTCGGCACAA AATTCGAAAA 
AATGTCACCT TTGCTGACGT GGAACTTGAT ATGTGTCAGA GACTCGTCCA TGAACCAGAA 
TCAATGGAGA GCAGGTCAAG TCCATATATT AGACCATCTC CATACGCTGG AGGAGTTCAT 
CCACACTTTG AAGAGGAAGA TGACAGGTCA AAACTTCATG CATCAGCTCT CTACTCGGAA 
CGCCGAACGT CATCCCGGAA ATCGTTAAGA TCACAAAAAA TAGACTATCA CACAACAACA 
ATAAAATCCC TTTGGTTTGA TTTTTGTGCC AGAACTTCTT CCCATGGAAT ACCATATGTT 
GCAACATCTT CATTTTTTGG CCGATATGTA TGGGCGGCAC TCTTCATGTG CATGTTAATG 
GCATTCTTAT TACAAACCTA TTGGACAATG TCAGAATATT TGCAGTATAG GACAATTATT 
GAAATGCAGT TGCAGTTTGA AGCTGCTGCA TTTCCCGCTG CAACAGTTTG CAATTTGAAT 
GCTTTCAAAT ATAGCGAGCT CACGCAGTAT GAAGAAATTA AAGAGGGTTT TGACTATTGG 
GAACGGGTGA TAAATGCTCG AATGATGAGT GACAGTATGA AGCCAGGAGG TGATATTCTA 
GAAGCAATCA GTGTCAGGAA AAAGCGATCA AAAAGTCGAG ATCAATTACT TTTTCCAATT 
GATGACGAAG ACTTAGAGGG TGCAGTGTAT CAGCCAGTCT TTGTGAGATG CACCTGTATG 
AATATGGAAC AATGTGTACC AAATAGGAAT CCTTTAGAGG TTAACGCCTC AATTTGTATG 
TGCTTTGAAG ACGTGACACG TGGCTTAATC TGGCCATGCT ACCCAACTTC CGTATGGACT 
GTAAAAAAAT GTTCTGGTTG TTCAATTTCC AATACTTGTC CTGACCCGGA TGGTCCTAAT 
GCTTCAAAGC AAATCGCAAA ACATAACAGT CCACTTCCAT GTCTTTGCCA ATCAATTTCT 
CATCATTGTA TGGTACATCC AAAGGACGAA ATCCGATGGT GGAACCCAAA CAATTATACA 
GTTTATTCTG TAACTGAACC ACCGACCACA GAAATAACTG AAACCGAAGA AGCTTTTGGA 
TTGAGCGACC TCAAAGATGC GGGAGCCATC ACAACTCAAA CAAAAGAAAA TTTAATTTTC 
TTAGTGGCTG CTTTACCCCG TGAAACAAGA AGGAACCTGT CATACACCCT CAATGAGTTT 
GTTCTTCGAT GTTCGTTTAA TTCGAAGGAT TGTAGTATGG AGCGTGACTT TAAACTTCAC 
GTTGATCCTG AATACGGCAA TTGCTACACA TTTAACTTCA ATGATTCGGT GGAGTTGAAA 
AACAGTAGAG CTGGTCCCAT GTATGGTCTC CGTCTATTAT TAAACGTGCA TCAAAGCGAT 
TACATGCCAA CAACAGAAGC AGCAGGTGTT CGATTAGTTG TCCATGAACA AGATCAAGAA 
CCGTTCCCTG ATACATTTGG ATACAGTGCT CCAACTGGAT TCATTTCTTC ATTTGGTCTT 
AAAACGAAAG AACTACATCG TCTCAGTGCT CCATGGGGGA ATTGCAGTGA CACATTCCGA 
CCGGTTCCAT ACATTTACAA CGAACACTAT TCTCCTGAAG GTTGCCATCG AAATTGTTTC 
CAATTGAAAG TACTTGAAAT ATGTGGATGT GGAGATCCAA GATTCCCTCT ACCATCTGAA 
GAGCATAGAC ATTGTAATGC GAAGTCAAAA ATTGATCGTC AATGTTTATC AAACCTAACA 
AGTGACTCTG GAGGTTACCA CCATCTACAC GAACAATGTG AATGTCGTCA GCCATGTCAT 
GAAAAAGTAT TTGAGACTGC TTACTCTGCA TCTGCATGGC CCAGTCAAAA TTTCAAAATT 
GGTACCGACT GCCCCGCCGT ATCAGATATT TTCAACGACA CTGAAGCTTG TACCGAATAT 
TATCGTCAAA ACACTGCTTA CATTGAAATT TACTACGAAC AATTGAATTT CGAATCGCTG 
AAAGAAACCG CCGGTTACAC GCTCGTCAAT CTTTTCTCAG ATTTTGGAGG TAATATTGGA 
TTATGGATTG GATTTTCAGT CATTACGTTT GCTGAATTTG CAGAACTTTT CTGCGAGATC 
TGCAAACTAA TGTATTTCAA AGGAATAGTC TATGTACAGA AAAAGATGCA AGGAAAAGAA 
TACACATCAT CTTCATTAAT GCACATTGAT TTTCTTCAAC GATCGCCTAA AAAAAGCCAA 
CCAGGAGAAG ATGAAGTTTC AACAAATGAA TCAACAAAGG AGTTAATGAG CAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-8 ( NM_001307365.1 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294294.1 Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.

Price & Availability↑

CloneID	OCa353359
Clone ID Related Accession (Same CDS sequence)	NM_001307365.1 , NM_001307365.2
Accession Version	NM_001307365.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	Degenerin unc-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). COMPLETENESS: incomplete on both ends.

ATGGAGAGCA GGTCAAGTCC ATATATTAGA CCATCTCCAT ACGCTGGAGG AGTTCATCCA 
CACTTTGAAG AGGAAGATGA CAGGTCAAAA CTTCATGCAT CAGCTCTCTA CTCGGAACGC 
CGAACGTCAT CCCGGAAATC GTTAAGATCA CAAAAAATAG ACTATCACAC AACAACAATA 
AAATCCCTTT GGTTTGATTT TTGTGCCAGA ACTTCTTCCC ATGGAATACC ATATGTTGCA 
ACATCTTCAT TTTTTGGCCG ATATGTATGG GCGGCACTCT TCATGTGCAT GTTAATGGCA 
TTCTTATTAC AAACCTATTG GACAATGTCA GAATATTTGC AGTATAGGAC AATTATTGAA 
ATGCAGTTGC AGTTTGAAGC TGCTGCATTT CCCGCTGCAA CAGTTTGCAA TTTGAATGCT 
TTCAAATATA GCGAGCTCAC GCAGTATGAA GAAATTAAAG AGGGTTTTGA CTATTGGGAA 
CGGGTGATAA ATGCTCGAAT GATGAGTGAC AGTATGAAGC CAGGAGGTGA TATTCTAGAA 
GCAATCAGTG TCAGGAAAAA GCGATCAAAA AGTCGAGATC AATTACTTTT TCCAATTGAT 
GACGAAGACT TAGAGGGTGC AGTGTATCAG CCAGTCTTTG TGAGATGCAC CTGTATGAAT 
ATGGAACAAT GTGTACCAAA TAGGAATCCT TTAGAGGTTA ACGCCTCAAT TTGTATGTGC 
TTTGAAGACG TGACACGTGG CTTAATCTGG CCATGCTACC CAACTTCCGT ATGGACTGTA 
AAAAAATGTT CTGGTTGTTC AATTTCCAAT ACTTGTCCTG ACCCGGATGG TCCTAATGCT 
TCAAAGCAAA TCGCAAAACA TAACAGTCCA CTTCCATGTC TTTGCCAATC AATTTCTCAT 
CATTGTATGG TACATCCAAA GGACGAAATC CGATGGTGGA ACCCAAACAA TTATACAGTT 
TATTCTGTAA CTGAACCACC GACCACAGAA ATAACTGAAA CCGAAGAAGC TTTTGGATTG 
AGCGACCTCA AAGATGCGGG AGCCATCACA ACTCAAACAA AAGAAAATTT AATTTTCTTA 
GTGGCTGCTT TACCCCGTGA AACAAGAAGG AACCTGTCAT ACACCCTCAA TGAGTTTGTT 
CTTCGATGTT CGTTTAATTC GAAGGATTGT AGTATGGAGC GTGACTTTAA ACTTCACGTT 
GATCCTGAAT ACGGCAATTG CTACACATTT AACTTCAATG ATTCGGTGGA GTTGAAAAAC 
AGTAGAGCTG GTCCCATGTA TGGTCTCCGT CTATTATTAA ACGTGCATCA AAGCGATTAC 
ATGCCAACAA CAGAAGCAGC AGGTGTTCGA TTAGTTGTCC ATGAACAAGA TCAAGAACCG 
TTCCCTGATA CATTTGGATA CAGTGCTCCA ACTGGATTCA TTTCTTCATT TGGTCTTAAA 
ACGAAAGAAC TACATCGTCT CAGTGCTCCA TGGGGGAATT GCAGTGACAC ATTCCGACCG 
GTTCCATACA TTTACAACGA ACACTATTCT CCTGAAGGTT GCCATCGAAA TTGTTTCCAA 
TTGAAAGTAC TTGAAATATG TGGATGTGGA GATCCAAGAT TCCCTCTACC ATCTGAAGAG 
CATAGACATT GTAATGCGAA GTCAAAAATT GATCGTCAAT GTTTATCAAA CCTAACAAGT 
GACTCTGGAG GTTACCACCA TCTACACGAA CAATGTGAAT GTCGTCAGCC ATGTCATGAA 
AAAGTATTTG AGACTGCTTA CTCTGCATCT GCATGGCCCA GTCAAAATTT CAAAATTGGT 
ACCGACTGCC CCGCCGTATC AGATATTTTC AACGACACTG AAGCTTGTAC CGAATATTAT 
CGTCAAAACA CTGCTTACAT TGAAATTTAC TACGAACAAT TGAATTTCGA ATCGCTGAAA 
GAAACCGCCG GTTACACGCT CGTCAATCTT TTCTCAGATT TTGGAGGTAA TATTGGATTA 
TGGATTGGAT TTTCAGTCAT TACGTTTGCT GAATTTGCAG AACTTTTCTG CGAGATCTGC 
AAACTAATGT ATTTCAAAGG AATAGTCTAT GTACAGAAAA AGATGCAAGG AAAAGAATAC 
ACATCATCTT CATTAATGCA CATTGATTTT CTTCAACGAT CGCCTAAAAA AAGCCAACCA 
GGAGAAGATG AAGTTTCAAC AAATGAATCA ACAAAGGAGT TAATGAGCAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294294.1
CDS	1..2214
Translation	MESRSSPYIRPSPYAGGVHPHFEEEDDRSKLHASALYSERRTSSRKSLRSQKIDYHTTTIKSLWFDFCARTSSHGIPYVATSSFFGRYVWAALFMCMLMAFLLQTYWTMSEYLQYRTIIEMQLQFEAAAFPAATVCNLNAFKYSELTQYEEIKEGFDYWERVINARMMSDSMKPGGDILEAISVRKKRSKSRDQLLFPIDDEDLEGAVYQPVFVRCTCMNMEQCVPNRNPLEVNASICMCFEDVTRGLIWPCYPTSVWTVKKCSGCSISNTCPDPDGPNASKQIAKHNSPLPCLCQSISHHCMVHPKDEIRWWNPNNYTVYSVTEPPTTEITETEEAFGLSDLKDAGAITTQTKENLIFLVAALPRETRRNLSYTLNEFVLRCSFNSKDCSMERDFKLHVDPEYGNCYTFNFNDSVELKNSRAGPMYGLRLLLNVHQSDYMPTTEAAGVRLVVHEQDQEPFPDTFGYSAPTGFISSFGLKTKELHRLSAPWGNCSDTFRPVPYIYNEHYSPEGCHRNCFQLKVLEICGCGDPRFPLPSEEHRHCNAKSKIDRQCLSNLTSDSGGYHHLHEQCECRQPCHEKVFETAYSASAWPSQNFKIGTDCPAVSDIFNDTEACTEYYRQNTAYIEIYYEQLNFESLKETAGYTLVNLFSDFGGNIGLWIGFSVITFAEFAELFCEICKLMYFKGIVYVQKKMQGKEYTSSSLMHIDFLQRSPKKSQPGEDEVSTNESTKELMSK

Target ORF information:

RefSeq Version	NM_001307365.1
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Degenerin unc-8 (unc-8), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307365.1

ORF Insert Sequence:

ATGGAGAGCA GGTCAAGTCC ATATATTAGA CCATCTCCAT ACGCTGGAGG AGTTCATCCA 
CACTTTGAAG AGGAAGATGA CAGGTCAAAA CTTCATGCAT CAGCTCTCTA CTCGGAACGC 
CGAACGTCAT CCCGGAAATC GTTAAGATCA CAAAAAATAG ACTATCACAC AACAACAATA 
AAATCCCTTT GGTTTGATTT TTGTGCCAGA ACTTCTTCCC ATGGAATACC ATATGTTGCA 
ACATCTTCAT TTTTTGGCCG ATATGTATGG GCGGCACTCT TCATGTGCAT GTTAATGGCA 
TTCTTATTAC AAACCTATTG GACAATGTCA GAATATTTGC AGTATAGGAC AATTATTGAA 
ATGCAGTTGC AGTTTGAAGC TGCTGCATTT CCCGCTGCAA CAGTTTGCAA TTTGAATGCT 
TTCAAATATA GCGAGCTCAC GCAGTATGAA GAAATTAAAG AGGGTTTTGA CTATTGGGAA 
CGGGTGATAA ATGCTCGAAT GATGAGTGAC AGTATGAAGC CAGGAGGTGA TATTCTAGAA 
GCAATCAGTG TCAGGAAAAA GCGATCAAAA AGTCGAGATC AATTACTTTT TCCAATTGAT 
GACGAAGACT TAGAGGGTGC AGTGTATCAG CCAGTCTTTG TGAGATGCAC CTGTATGAAT 
ATGGAACAAT GTGTACCAAA TAGGAATCCT TTAGAGGTTA ACGCCTCAAT TTGTATGTGC 
TTTGAAGACG TGACACGTGG CTTAATCTGG CCATGCTACC CAACTTCCGT ATGGACTGTA 
AAAAAATGTT CTGGTTGTTC AATTTCCAAT ACTTGTCCTG ACCCGGATGG TCCTAATGCT 
TCAAAGCAAA TCGCAAAACA TAACAGTCCA CTTCCATGTC TTTGCCAATC AATTTCTCAT 
CATTGTATGG TACATCCAAA GGACGAAATC CGATGGTGGA ACCCAAACAA TTATACAGTT 
TATTCTGTAA CTGAACCACC GACCACAGAA ATAACTGAAA CCGAAGAAGC TTTTGGATTG 
AGCGACCTCA AAGATGCGGG AGCCATCACA ACTCAAACAA AAGAAAATTT AATTTTCTTA 
GTGGCTGCTT TACCCCGTGA AACAAGAAGG AACCTGTCAT ACACCCTCAA TGAGTTTGTT 
CTTCGATGTT CGTTTAATTC GAAGGATTGT AGTATGGAGC GTGACTTTAA ACTTCACGTT 
GATCCTGAAT ACGGCAATTG CTACACATTT AACTTCAATG ATTCGGTGGA GTTGAAAAAC 
AGTAGAGCTG GTCCCATGTA TGGTCTCCGT CTATTATTAA ACGTGCATCA AAGCGATTAC 
ATGCCAACAA CAGAAGCAGC AGGTGTTCGA TTAGTTGTCC ATGAACAAGA TCAAGAACCG 
TTCCCTGATA CATTTGGATA CAGTGCTCCA ACTGGATTCA TTTCTTCATT TGGTCTTAAA 
ACGAAAGAAC TACATCGTCT CAGTGCTCCA TGGGGGAATT GCAGTGACAC ATTCCGACCG 
GTTCCATACA TTTACAACGA ACACTATTCT CCTGAAGGTT GCCATCGAAA TTGTTTCCAA 
TTGAAAGTAC TTGAAATATG TGGATGTGGA GATCCAAGAT TCCCTCTACC ATCTGAAGAG 
CATAGACATT GTAATGCGAA GTCAAAAATT GATCGTCAAT GTTTATCAAA CCTAACAAGT 
GACTCTGGAG GTTACCACCA TCTACACGAA CAATGTGAAT GTCGTCAGCC ATGTCATGAA 
AAAGTATTTG AGACTGCTTA CTCTGCATCT GCATGGCCCA GTCAAAATTT CAAAATTGGT 
ACCGACTGCC CCGCCGTATC AGATATTTTC AACGACACTG AAGCTTGTAC CGAATATTAT 
CGTCAAAACA CTGCTTACAT TGAAATTTAC TACGAACAAT TGAATTTCGA ATCGCTGAAA 
GAAACCGCCG GTTACACGCT CGTCAATCTT TTCTCAGATT TTGGAGGTAA TATTGGATTA 
TGGATTGGAT TTTCAGTCAT TACGTTTGCT GAATTTGCAG AACTTTTCTG CGAGATCTGC 
AAACTAATGT ATTTCAAAGG AATAGTCTAT GTACAGAAAA AGATGCAAGG AAAAGAATAC 
ACATCATCTT CATTAATGCA CATTGATTTT CTTCAACGAT CGCCTAAAAA AAGCCAACCA 
GGAGAAGATG AAGTTTCAAC AAATGAATCA ACAAAGGAGT TAATGAGCAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

unc-8 ( NM_001307365.2 ) cDNA ORF clone, Caenorhabditis elegans -> NP_001294294.1 Caenorhabditis elegans Degenerin unc-8 (unc-8), mRNA.

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CloneID	OCa353359
Clone ID Related Accession (Same CDS sequence)	NM_001307365.1 , NM_001307365.2
Accession Version	NM_001307365.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2214bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	Degenerin unc-8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003282). On Sep 4, 2019 this sequence version replaced NM_001307365.1.

ATGGAGAGCA GGTCAAGTCC ATATATTAGA CCATCTCCAT ACGCTGGAGG AGTTCATCCA 
CACTTTGAAG AGGAAGATGA CAGGTCAAAA CTTCATGCAT CAGCTCTCTA CTCGGAACGC 
CGAACGTCAT CCCGGAAATC GTTAAGATCA CAAAAAATAG ACTATCACAC AACAACAATA 
AAATCCCTTT GGTTTGATTT TTGTGCCAGA ACTTCTTCCC ATGGAATACC ATATGTTGCA 
ACATCTTCAT TTTTTGGCCG ATATGTATGG GCGGCACTCT TCATGTGCAT GTTAATGGCA 
TTCTTATTAC AAACCTATTG GACAATGTCA GAATATTTGC AGTATAGGAC AATTATTGAA 
ATGCAGTTGC AGTTTGAAGC TGCTGCATTT CCCGCTGCAA CAGTTTGCAA TTTGAATGCT 
TTCAAATATA GCGAGCTCAC GCAGTATGAA GAAATTAAAG AGGGTTTTGA CTATTGGGAA 
CGGGTGATAA ATGCTCGAAT GATGAGTGAC AGTATGAAGC CAGGAGGTGA TATTCTAGAA 
GCAATCAGTG TCAGGAAAAA GCGATCAAAA AGTCGAGATC AATTACTTTT TCCAATTGAT 
GACGAAGACT TAGAGGGTGC AGTGTATCAG CCAGTCTTTG TGAGATGCAC CTGTATGAAT 
ATGGAACAAT GTGTACCAAA TAGGAATCCT TTAGAGGTTA ACGCCTCAAT TTGTATGTGC 
TTTGAAGACG TGACACGTGG CTTAATCTGG CCATGCTACC CAACTTCCGT ATGGACTGTA 
AAAAAATGTT CTGGTTGTTC AATTTCCAAT ACTTGTCCTG ACCCGGATGG TCCTAATGCT 
TCAAAGCAAA TCGCAAAACA TAACAGTCCA CTTCCATGTC TTTGCCAATC AATTTCTCAT 
CATTGTATGG TACATCCAAA GGACGAAATC CGATGGTGGA ACCCAAACAA TTATACAGTT 
TATTCTGTAA CTGAACCACC GACCACAGAA ATAACTGAAA CCGAAGAAGC TTTTGGATTG 
AGCGACCTCA AAGATGCGGG AGCCATCACA ACTCAAACAA AAGAAAATTT AATTTTCTTA 
GTGGCTGCTT TACCCCGTGA AACAAGAAGG AACCTGTCAT ACACCCTCAA TGAGTTTGTT 
CTTCGATGTT CGTTTAATTC GAAGGATTGT AGTATGGAGC GTGACTTTAA ACTTCACGTT 
GATCCTGAAT ACGGCAATTG CTACACATTT AACTTCAATG ATTCGGTGGA GTTGAAAAAC 
AGTAGAGCTG GTCCCATGTA TGGTCTCCGT CTATTATTAA ACGTGCATCA AAGCGATTAC 
ATGCCAACAA CAGAAGCAGC AGGTGTTCGA TTAGTTGTCC ATGAACAAGA TCAAGAACCG 
TTCCCTGATA CATTTGGATA CAGTGCTCCA ACTGGATTCA TTTCTTCATT TGGTCTTAAA 
ACGAAAGAAC TACATCGTCT CAGTGCTCCA TGGGGGAATT GCAGTGACAC ATTCCGACCG 
GTTCCATACA TTTACAACGA ACACTATTCT CCTGAAGGTT GCCATCGAAA TTGTTTCCAA 
TTGAAAGTAC TTGAAATATG TGGATGTGGA GATCCAAGAT TCCCTCTACC ATCTGAAGAG 
CATAGACATT GTAATGCGAA GTCAAAAATT GATCGTCAAT GTTTATCAAA CCTAACAAGT 
GACTCTGGAG GTTACCACCA TCTACACGAA CAATGTGAAT GTCGTCAGCC ATGTCATGAA 
AAAGTATTTG AGACTGCTTA CTCTGCATCT GCATGGCCCA GTCAAAATTT CAAAATTGGT 
ACCGACTGCC CCGCCGTATC AGATATTTTC AACGACACTG AAGCTTGTAC CGAATATTAT 
CGTCAAAACA CTGCTTACAT TGAAATTTAC TACGAACAAT TGAATTTCGA ATCGCTGAAA 
GAAACCGCCG GTTACACGCT CGTCAATCTT TTCTCAGATT TTGGAGGTAA TATTGGATTA 
TGGATTGGAT TTTCAGTCAT TACGTTTGCT GAATTTGCAG AACTTTTCTG CGAGATCTGC 
AAACTAATGT ATTTCAAAGG AATAGTCTAT GTACAGAAAA AGATGCAAGG AAAAGAATAC 
ACATCATCTT CATTAATGCA CATTGATTTT CTTCAACGAT CGCCTAAAAA AAGCCAACCA 
GGAGAAGATG AAGTTTCAAC AAATGAATCA ACAAAGGAGT TAATGAGCAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001294294.1
CDS	25..2238
Translation	MESRSSPYIRPSPYAGGVHPHFEEEDDRSKLHASALYSERRTSSRKSLRSQKIDYHTTTIKSLWFDFCARTSSHGIPYVATSSFFGRYVWAALFMCMLMAFLLQTYWTMSEYLQYRTIIEMQLQFEAAAFPAATVCNLNAFKYSELTQYEEIKEGFDYWERVINARMMSDSMKPGGDILEAISVRKKRSKSRDQLLFPIDDEDLEGAVYQPVFVRCTCMNMEQCVPNRNPLEVNASICMCFEDVTRGLIWPCYPTSVWTVKKCSGCSISNTCPDPDGPNASKQIAKHNSPLPCLCQSISHHCMVHPKDEIRWWNPNNYTVYSVTEPPTTEITETEEAFGLSDLKDAGAITTQTKENLIFLVAALPRETRRNLSYTLNEFVLRCSFNSKDCSMERDFKLHVDPEYGNCYTFNFNDSVELKNSRAGPMYGLRLLLNVHQSDYMPTTEAAGVRLVVHEQDQEPFPDTFGYSAPTGFISSFGLKTKELHRLSAPWGNCSDTFRPVPYIYNEHYSPEGCHRNCFQLKVLEICGCGDPRFPLPSEEHRHCNAKSKIDRQCLSNLTSDSGGYHHLHEQCECRQPCHEKVFETAYSASAWPSQNFKIGTDCPAVSDIFNDTEACTEYYRQNTAYIEIYYEQLNFESLKETAGYTLVNLFSDFGGNIGLWIGFSVITFAEFAELFCEICKLMYFKGIVYVQKKMQGKEYTSSSLMHIDFLQRSPKKSQPGEDEVSTNESTKELMSK

Target ORF information:

RefSeq Version	NM_001307365.2
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans Degenerin unc-8 (unc-8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001307365.2

ORF Insert Sequence:

ATGGAGAGCA GGTCAAGTCC ATATATTAGA CCATCTCCAT ACGCTGGAGG AGTTCATCCA 
CACTTTGAAG AGGAAGATGA CAGGTCAAAA CTTCATGCAT CAGCTCTCTA CTCGGAACGC 
CGAACGTCAT CCCGGAAATC GTTAAGATCA CAAAAAATAG ACTATCACAC AACAACAATA 
AAATCCCTTT GGTTTGATTT TTGTGCCAGA ACTTCTTCCC ATGGAATACC ATATGTTGCA 
ACATCTTCAT TTTTTGGCCG ATATGTATGG GCGGCACTCT TCATGTGCAT GTTAATGGCA 
TTCTTATTAC AAACCTATTG GACAATGTCA GAATATTTGC AGTATAGGAC AATTATTGAA 
ATGCAGTTGC AGTTTGAAGC TGCTGCATTT CCCGCTGCAA CAGTTTGCAA TTTGAATGCT 
TTCAAATATA GCGAGCTCAC GCAGTATGAA GAAATTAAAG AGGGTTTTGA CTATTGGGAA 
CGGGTGATAA ATGCTCGAAT GATGAGTGAC AGTATGAAGC CAGGAGGTGA TATTCTAGAA 
GCAATCAGTG TCAGGAAAAA GCGATCAAAA AGTCGAGATC AATTACTTTT TCCAATTGAT 
GACGAAGACT TAGAGGGTGC AGTGTATCAG CCAGTCTTTG TGAGATGCAC CTGTATGAAT 
ATGGAACAAT GTGTACCAAA TAGGAATCCT TTAGAGGTTA ACGCCTCAAT TTGTATGTGC 
TTTGAAGACG TGACACGTGG CTTAATCTGG CCATGCTACC CAACTTCCGT ATGGACTGTA 
AAAAAATGTT CTGGTTGTTC AATTTCCAAT ACTTGTCCTG ACCCGGATGG TCCTAATGCT 
TCAAAGCAAA TCGCAAAACA TAACAGTCCA CTTCCATGTC TTTGCCAATC AATTTCTCAT 
CATTGTATGG TACATCCAAA GGACGAAATC CGATGGTGGA ACCCAAACAA TTATACAGTT 
TATTCTGTAA CTGAACCACC GACCACAGAA ATAACTGAAA CCGAAGAAGC TTTTGGATTG 
AGCGACCTCA AAGATGCGGG AGCCATCACA ACTCAAACAA AAGAAAATTT AATTTTCTTA 
GTGGCTGCTT TACCCCGTGA AACAAGAAGG AACCTGTCAT ACACCCTCAA TGAGTTTGTT 
CTTCGATGTT CGTTTAATTC GAAGGATTGT AGTATGGAGC GTGACTTTAA ACTTCACGTT 
GATCCTGAAT ACGGCAATTG CTACACATTT AACTTCAATG ATTCGGTGGA GTTGAAAAAC 
AGTAGAGCTG GTCCCATGTA TGGTCTCCGT CTATTATTAA ACGTGCATCA AAGCGATTAC 
ATGCCAACAA CAGAAGCAGC AGGTGTTCGA TTAGTTGTCC ATGAACAAGA TCAAGAACCG 
TTCCCTGATA CATTTGGATA CAGTGCTCCA ACTGGATTCA TTTCTTCATT TGGTCTTAAA 
ACGAAAGAAC TACATCGTCT CAGTGCTCCA TGGGGGAATT GCAGTGACAC ATTCCGACCG 
GTTCCATACA TTTACAACGA ACACTATTCT CCTGAAGGTT GCCATCGAAA TTGTTTCCAA 
TTGAAAGTAC TTGAAATATG TGGATGTGGA GATCCAAGAT TCCCTCTACC ATCTGAAGAG 
CATAGACATT GTAATGCGAA GTCAAAAATT GATCGTCAAT GTTTATCAAA CCTAACAAGT 
GACTCTGGAG GTTACCACCA TCTACACGAA CAATGTGAAT GTCGTCAGCC ATGTCATGAA 
AAAGTATTTG AGACTGCTTA CTCTGCATCT GCATGGCCCA GTCAAAATTT CAAAATTGGT 
ACCGACTGCC CCGCCGTATC AGATATTTTC AACGACACTG AAGCTTGTAC CGAATATTAT 
CGTCAAAACA CTGCTTACAT TGAAATTTAC TACGAACAAT TGAATTTCGA ATCGCTGAAA 
GAAACCGCCG GTTACACGCT CGTCAATCTT TTCTCAGATT TTGGAGGTAA TATTGGATTA 
TGGATTGGAT TTTCAGTCAT TACGTTTGCT GAATTTGCAG AACTTTTCTG CGAGATCTGC 
AAACTAATGT ATTTCAAAGG AATAGTCTAT GTACAGAAAA AGATGCAAGG AAAAGAATAC 
ACATCATCTT CATTAATGCA CATTGATTTT CTTCAACGAT CGCCTAAAAA AAGCCAACCA 
GGAGAAGATG AAGTTTCAAC AAATGAATCA ACAAAGGAGT TAATGAGCAA ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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unc-8 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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