Scnn1g cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Scnn1g
Entrez Gene ID	24768
Full Name	sodium channel epithelial 1 gamma subunit
Synonyms	ENaC
General protein information	Preferred Names sodium channel epithelial 1 gamma subunit Names amiloride-sensitive sodium channel subunit gamma SCNEG Sodium channel, nonvoltage-gated 1, gamma (epithelial) amiloride sensitive sodium channel gamma1 subunit epithelial Na(+) channel subunit gamma epithelial sodium channel gamma subunit gamma-ENaC gamma-NaCH nonvoltage-gated sodium channel 1 subunit gamma sodium channel, non-voltage-gated 1, gamma subunit sodium channel, nonvoltage-gated, type I, gamma
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q36
Summary	acts as an epithelial sodium ion channel; regulates salt and fluid transport in the kidney [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_017046.2	NP_058742.2	amiloride-sensitive sodium channel subunit gamma
NM_017046.1	NP_058742.1	amiloride-sensitive sodium channel subunit gamma

Pathways from BioSystems

Homologies

	SCNN1G	XP_001093466.1
Mus musculus (house mouse)	Scnn1g	NP_035456.1
Caenorhabditis elegans (roundworm)	asic-1	NP_491214.3
Caenorhabditis elegans (roundworm)	mec-4	NP_510712.2
Caenorhabditis elegans (roundworm)	del-1	NP_510380.1
Caenorhabditis elegans (roundworm)	unc-8	NP_501138.1
Xenopus tropicalis (tropical clawed frog)	scnn1g	XP_004918046.1
Pan troglodytes (chimpanzee)	SCNN1G	XP_003315070.1
Caenorhabditis elegans (roundworm)	unc-105	NP_001254142.1
Caenorhabditis elegans (roundworm)	asic-2	NP_492099.1
Caenorhabditis elegans (roundworm)	mec-10	NP_509438.1
Bos taurus (cattle)	SCNN1G	NP_001180103.1
Gallus gallus (chicken)	SCNN1G	XP_414880.2
Homo sapiens (human)	SCNN1G	NP_001030.2
Rattus norvegicus (Norway rat)	Scnn1g	NP_058742.1
Canis lupus familiaris (dog)	SCNN1G	XP_547093.2
Caenorhabditis elegans (roundworm)	deg-1	NP_509311.1
Caenorhabditis elegans (roundworm)	del-4	NP_492230.2

Gene Ontology
Bibliography

Related articles in PubMed

Changes in plasma aldosterone and electrolytes levels, kidney epithelial sodium channel (ENaC) and blood pressure in normotensive WKY and hypertensive SHR rats following gonadectomy and chronic testosterone treatment.
Loh SY, Giribabu N, Salleh N
Steroids128128-135(2017 Dec)

Involvement of Epithelial Na
Choi SK, Yeon SI, Kwon Y, Byeon S, Lee YH
Scientific reports745996(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Kidneys respond acutely to hyperkalemic challenges by decreasing the activity of NCC while increasing that of ENaC.
Title: Responses of distal nephron Na

This study demonstrated that the formation of the heminodal Nav channel cluster adjacent to the presynaptic terminal occurs around hearing onset in the second week of postnatal age.
Title: Activity-dependent formation and location of voltage-gated sodium channel clusters at a CNS nerve terminal during postnatal development.

deltabetagamma-ENaC is inhibited by CFTR but activated by cyclic AMP.
Title: ???-ENaC is inhibited by CFTR but stimulated by cAMP in

Data suggest that dietary factors can regulate cardiac Scnn1g, Nedd4-2, and Sgk1 activity; here, proanthocyanidin dietary supplement PRO80 blocks aldosterone-dependent up-regulation of Scnn1g, Nedd4-2, and Sgk1 content in cardiac dysfunction. (Scnn1g = epithelial sodium channel 1, gamma subunit; Nedd4-2 = ubiquitin-protein ligase Nedd4-2; Sgk1 = serum-glucocorticoid regulated kinase 1)
Title: Proanthocyanidins block aldosterone-dependent up-regulation of cardiac gamma ENaC and Nedd4-2 inactivation via SGK1.

Decreased expression of Scnn1b/g and activation of ENaC could be involved in attenuation of the aortic baroreceptor sensitivity in congestive heart failure.
Title: Altered ENaC is Associated With Aortic Baroreceptor Dysfunction in Chronic Heart Failure.

The following Scnn1g gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Scnn1g cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12915	NM_017046.1 Latest version!	Rattus norvegicus sodium channel epithelial 1 gamma subunit (Scnn1g), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$384.30 $549.00
ORa63311	NM_017046.2 Latest version!	Rattus norvegicus sodium channel epithelial 1 subunit gamma (Scnn1g), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Scnn1g ( NM_017046.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058742.1 Rattus norvegicus sodium channel epithelial 1 gamma subunit (Scnn1g), mRNA.

Price & Availability↑

CloneID	ORa12915
Clone ID Related Accession (Same CDS sequence)	NM_017046.1
Accession Version	NM_017046.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-11
Organism	Rattus norvegicus(Norway rat)
Product	amiloride-sensitive sodium channel subunit gamma
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from X77933.1. Sequence Note: This sequence has been modified as follows: removed 25 bp suspected to be vector contamination from the 3' end. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X77933.1, U37539.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCCTG GAGAGAAGAT CAAAGCCAAA ATCAAAAAGA ATCTGCCGGT TCGAGGCCCC 
CAGGCACCAA CCATTAAGGA CCTGATGCAT TGGTACTGCA TGAACACCAA CACCCACGGG 
TGCCGCCGCA TCGTGGTGTC CCGAGGCCGC CTTCGGCCTC TGCTGTGGAT CGCGTTCACG 
CTAACTGCAG TGGCCCTCAT TATCTGGCAG TGCGCCCTCC TCGTCTTCTC TTTCTACACC 
GTCTCTGTCT CCATCAAAGT CCACTTCCAG AAACTGGATT TCCCTGCCGT CACTATCTGC 
AATATCAACC CTTACAAGTA CAGTGCTGTG AGTGACCTTC TGACTGACTT GGACAGTGAG 
ACAAAACAGG CCTTGCTGTC CTTGTATGGG GTCAAAGAAT CTCGGAAACG CCGGGAAGCA 
GGATCCATGC CGTCCACCTT GGAAGGCACA CCACCCAGAT TCTTCAAACT GATCCCACTG 
CTGGTCTTCA ATGAGAATGA GAAGGGAAAG GCCAGGGACT TCTTCACTGG CCGGAAGCGG 
AAAATCAGCG GGAAAATCAT TCACAAAGCT TCTAATGTCA TGCACGTTCA TGAGTCGAAG 
AAACTGGTGG GATTTCAATT GTGCTCAAAT GATACCTCTG ACTGCGCCAC CTACACCTTC 
AGCTCAGGAA TCAATGCCAT CCAGGAGTGG TACAAGCTTC ACTACATGAA TATCATGGCA 
CAGGTGCCTC TAGAGAAGAA AATCAACATG AGCTATTCTG CCGAAGAACT GCTGGTGACC 
TGCTTCTTCG ATGGGATGTC CTGTGATGCC AGGAACTTCA CGCTTTTCCA CCATCCAATG 
TATGGGAACT GCTACACATT TAACAACAAA GAAAATGCCA CCATCCTCAG CACCTCCATG 
GGAGGCAGTG AGTACGGGCT GCAAGTCATC TTATACATAA ACGAAGATGA ATACAACCCC 
TTCCTGGTGT CCTCCACTGG AGCCAAGGTG CTTATCCATC AGCAGAATGA ATACCCCTTC 
ATCGAAGACG TGGGGATGGA GATCGAGACA GCAATGTCCA CTTCCATAGG AATGCACCTG 
ACAGAATCCT TCAAGCTGAG CGAACCTTAC AGCCAGTGCA CAGAGGACGG CAGTGATGTG 
CCCGTCACAA ACATCTACAA CGCTGCCTAC TCCCTCCAGA TCTGCCTTTA TTCATGCTTC 
CAAACAAAGA TGGTGGAAAA ATGTGGCTGC GCCCAGTACA GCCAGCCTCT GCCTCCAGCA 
GCCAATTACT GCAACTACCA GCAACACCCC AACTGGATGT ATTGCTACTA CCAGTTGTAC 
CAGGCCTTTG TCCGGGAAGA ACTGGGCTGC CAATCAGTGT GCAAGCAATC CTGTAGCTTT 
AAGGAATGGA CACTGACCAC CAGCTTGGCA CAATGGCCGT CTGAGGCTTC CGAGAAATGG 
TTGCTGAATG TTCTCACCTG GGACCAAAGC CAGCAAATAA ACAAAAAGCT CAACAAGACT 
GACCTGGCCA AGCTCTTGAT ATTCTACAAA GACCTGAACC AAAGATCCAT CATGGAGAGC 
CCAGCCAACA GTATTGAGAT GCTCCTGTCC AACTTTGGTG GACAGCTCGG CCTGTGGATG 
AGCTGTTCAG TCGTCTGTGT CATTGAAATC ATTGAAGTCT TCTTCATTGA CTTCTTCTCT 
ATCATCGCCC GCCGTCAGTG GCACAAAGCC AAGGATTGTT GGGCCCGTAG GCAGACACCG 
CCCTCCACTG AGACTCCCTC CAGCCGGCAA GGCCAGGATA ATCCAGCTCT GGATACGGAC 
GATGACCTGC CCACTTTTAC CTCTGCTATG CGCCTGCCTC CAGCCCCGGG GTCCACGGTG 
CCTGGCACAC CACCTCCCAG ATACAATACC TTGCGCTTGG ATAGAGCCTT TTCATCCCAG 
CTCACAGACA CTCAGTTGAC CAATGAGTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058742.1
CDS	99..2051
Translation	MAPGEKIKAKIKKNLPVRGPQAPTIKDLMHWYCMNTNTHGCRRIVVSRGRLRPLLWIAFTLTAVALIIWQCALLVFSFYTVSVSIKVHFQKLDFPAVTICNINPYKYSAVSDLLTDLDSETKQALLSLYGVKESRKRREAGSMPSTLEGTPPRFFKLIPLLVFNENEKGKARDFFTGRKRKISGKIIHKASNVMHVHESKKLVGFQLCSNDTSDCATYTFSSGINAIQEWYKLHYMNIMAQVPLEKKINMSYSAEELLVTCFFDGMSCDARNFTLFHHPMYGNCYTFNNKENATILSTSMGGSEYGLQVILYINEDEYNPFLVSSTGAKVLIHQQNEYPFIEDVGMEIETAMSTSIGMHLTESFKLSEPYSQCTEDGSDVPVTNIYNAAYSLQICLYSCFQTKMVEKCGCAQYSQPLPPAANYCNYQQHPNWMYCYYQLYQAFVREELGCQSVCKQSCSFKEWTLTTSLAQWPSEASEKWLLNVLTWDQSQQINKKLNKTDLAKLLIFYKDLNQRSIMESPANSIEMLLSNFGGQLGLWMSCSVVCVIEIIEVFFIDFFSIIARRQWHKAKDCWARRQTPPSTETPSSRQGQDNPALDTDDDLPTFTSAMRLPPAPGSTVPGTPPPRYNTLRLDRAFSSQLTDTQLTNEL

Target ORF information:

RefSeq Version	NM_017046.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium channel epithelial 1 gamma subunit (Scnn1g), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017046.1

ORF Insert Sequence:

ATGGCGCCTG GAGAGAAGAT CAAAGCCAAA ATCAAAAAGA ATCTGCCGGT TCGAGGCCCC 
CAGGCACCAA CCATTAAGGA CCTGATGCAT TGGTACTGCA TGAACACCAA CACCCACGGG 
TGCCGCCGCA TCGTGGTGTC CCGAGGCCGC CTTCGGCCTC TGCTGTGGAT CGCGTTCACG 
CTAACTGCAG TGGCCCTCAT TATCTGGCAG TGCGCCCTCC TCGTCTTCTC TTTCTACACC 
GTCTCTGTCT CCATCAAAGT CCACTTCCAG AAACTGGATT TCCCTGCCGT CACTATCTGC 
AATATCAACC CTTACAAGTA CAGTGCTGTG AGTGACCTTC TGACTGACTT GGACAGTGAG 
ACAAAACAGG CCTTGCTGTC CTTGTATGGG GTCAAAGAAT CTCGGAAACG CCGGGAAGCA 
GGATCCATGC CGTCCACCTT GGAAGGCACA CCACCCAGAT TCTTCAAACT GATCCCACTG 
CTGGTCTTCA ATGAGAATGA GAAGGGAAAG GCCAGGGACT TCTTCACTGG CCGGAAGCGG 
AAAATCAGCG GGAAAATCAT TCACAAAGCT TCTAATGTCA TGCACGTTCA TGAGTCGAAG 
AAACTGGTGG GATTTCAATT GTGCTCAAAT GATACCTCTG ACTGCGCCAC CTACACCTTC 
AGCTCAGGAA TCAATGCCAT CCAGGAGTGG TACAAGCTTC ACTACATGAA TATCATGGCA 
CAGGTGCCTC TAGAGAAGAA AATCAACATG AGCTATTCTG CCGAAGAACT GCTGGTGACC 
TGCTTCTTCG ATGGGATGTC CTGTGATGCC AGGAACTTCA CGCTTTTCCA CCATCCAATG 
TATGGGAACT GCTACACATT TAACAACAAA GAAAATGCCA CCATCCTCAG CACCTCCATG 
GGAGGCAGTG AGTACGGGCT GCAAGTCATC TTATACATAA ACGAAGATGA ATACAACCCC 
TTCCTGGTGT CCTCCACTGG AGCCAAGGTG CTTATCCATC AGCAGAATGA ATACCCCTTC 
ATCGAAGACG TGGGGATGGA GATCGAGACA GCAATGTCCA CTTCCATAGG AATGCACCTG 
ACAGAATCCT TCAAGCTGAG CGAACCTTAC AGCCAGTGCA CAGAGGACGG CAGTGATGTG 
CCCGTCACAA ACATCTACAA CGCTGCCTAC TCCCTCCAGA TCTGCCTTTA TTCATGCTTC 
CAAACAAAGA TGGTGGAAAA ATGTGGCTGC GCCCAGTACA GCCAGCCTCT GCCTCCAGCA 
GCCAATTACT GCAACTACCA GCAACACCCC AACTGGATGT ATTGCTACTA CCAGTTGTAC 
CAGGCCTTTG TCCGGGAAGA ACTGGGCTGC CAATCAGTGT GCAAGCAATC CTGTAGCTTT 
AAGGAATGGA CACTGACCAC CAGCTTGGCA CAATGGCCGT CTGAGGCTTC CGAGAAATGG 
TTGCTGAATG TTCTCACCTG GGACCAAAGC CAGCAAATAA ACAAAAAGCT CAACAAGACT 
GACCTGGCCA AGCTCTTGAT ATTCTACAAA GACCTGAACC AAAGATCCAT CATGGAGAGC 
CCAGCCAACA GTATTGAGAT GCTCCTGTCC AACTTTGGTG GACAGCTCGG CCTGTGGATG 
AGCTGTTCAG TCGTCTGTGT CATTGAAATC ATTGAAGTCT TCTTCATTGA CTTCTTCTCT 
ATCATCGCCC GCCGTCAGTG GCACAAAGCC AAGGATTGTT GGGCCCGTAG GCAGACACCG 
CCCTCCACTG AGACTCCCTC CAGCCGGCAA GGCCAGGATA ATCCAGCTCT GGATACGGAC 
GATGACCTGC CCACTTTTAC CTCTGCTATG CGCCTGCCTC CAGCCCCGGG GTCCACGGTG 
CCTGGCACAC CACCTCCCAG ATACAATACC TTGCGCTTGG ATAGAGCCTT TTCATCCCAG 
CTCACAGACA CTCAGTTGAC CAATGAGTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scnn1g ( NM_017046.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_058742.2 Rattus norvegicus sodium channel epithelial 1 subunit gamma (Scnn1g), mRNA.

Price & Availability↑

CloneID	ORa63311
Clone ID Related Accession (Same CDS sequence)	NM_017046.2
Accession Version	NM_017046.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-10
Organism	Rattus norvegicus(Norway rat)
Product	amiloride-sensitive sodium channel subunit gamma
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07071976.1 and AC122986.4. On Sep 13, 2018 this sequence version replaced NM_017046.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X77933.1, X78034.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689597, SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGGCGCCTG GAGAGAAGAT CAAAGCCAAA ATCAAAAAGA ATCTGCCGGT TCGAGGCCCC 
CAGGCACCAA CCATTAAGGA CCTGATGCAT TGGTACTGCA TGAACACCAA CACCCACGGC 
TGCCGCCGCA TCGTGGTGTC CCGAGGCCGC CTTCGGCGCC TGCTGTGGAT CGCGTTCACG 
CTAACTGCAG TGGCCCTCAT TATCTGGCAG TGCGCCCTCC TCGTCTTCTC TTTCTACACC 
GTCTCTGTCT CCATCAAAGT CCACTTCCAG AAACTGGATT TCCCTGCCGT CACTATCTGC 
AATATCAACC CTTACAAGTA CAGTGCTGTG AGTGACCTTC TGACTGACTT GGACAGTGAG 
ACAAAACAGG CCTTGCTGTC CTTGTATGGG GTCAAAGAAT CTCGGAAACG CCGGGAAGCA 
GGATCCATGC CGTCCACCTT GGAAGGCACA CCACCCAGAT TCTTCAAACT GATCCCACTG 
CTGGTCTTCA ATGAGAATGA GAAGGGAAAG GCCAGGGACT TCTTCACTGG CCGGAAGCGG 
AAAATCAGCG GGAAAATCAT TCACAAAGCT TCTAATGTCA TGCACGTTCA TGAGTCGAAG 
AAACTGGTGG GATTTCAATT GTGCTCAAAT GATACCTCTG ACTGCGCCAC CTACACCTTC 
AGCTCAGGAA TCAATGCCAT CCAGGAGTGG TACAAGCTTC ACTACATGAA TATCATGGCA 
CAGGTGCCTC TAGAGAAGAA AATCAACATG AGCTATTCTG CCAAAGAACT GCTGGTGACC 
TGCTTCTTCG ATGGGATGTC CTGTGATGCC AGGAACTTCA CGCTTTTCCA CCATCCAATG 
TATGGGAACT GCTACACATT TAACAACAAA GAAAATGCCA CCATCCTCAG CACCTCCATG 
GGAGGCAGTG AGTACGGGCT GCAAGTCATC TTATACATAA ACGAAGATGA ATACAACCCC 
TTCCTGGTGT CCTCCACTGG AGCCAAGGTG CTTATCCATC AGCAGAATGA ATACCCCTTC 
ATCGAAGACG TGGGGATGGA GATCGAGACA GCAATGTCCA CTTCCATAGG AATGCACCTG 
ACAGAATCCT TCAAGCTGAG CGAACCTTAC AGCCAGTGCA CAGAGGATGG CAGTGATGTG 
CCCGTCACAA ACATCTACAA CGCTGCCTAC TCCCTCCAGA TCTGCCTTTA TTCATGCTTC 
CAAACAAAGA TGGTGGAAAA ATGTGGCTGT GCCCAGTACA GCCAGCCTCT GCCTCCAGCA 
GCCAATTACT GCAACTACCA GCAACACCCC AACTGGATGT ATTGCTACTA CCAGTTGTAC 
CAGGCCTTTG TCCGGGAAGA ACTGGGCTGC CAATCAGTGT GCAAGCAATC CTGTAGCTTT 
AAGGAATGGA CACTGACCAC CAGCTTGGCA CAATGGCCGT CTGAGGCTTC CGAGAAATGG 
TTGCTGAATG TTCTCACCTG GGACCAAAGC CAGCAAATAA ACAAAAAGCT CAACAAGACT 
GACCTGGCCA AGCTCTTGAT ATTCTACAAA GACCTGAACC AAAGATCCAT CATGGAGAGC 
CCAGCCAACA GTATTGAGAT GCTCCTGTCC AACTTTGGTG GACAGCTCGG CCTGTGGATG 
AGCTGCTCAG TCGTCTGTGT CATTGAAATC ATTGAAGTCT TCTTCATTGA CTTCTTCTCT 
ATCATCGCCC GCCGTCAGTG GCACAAAGCC AAGGATTGGT GGGCCCGTAG GCAGACACCG 
CCCTCCACTG AGACTCCCTC CAGCCGGCAA GGCCAGGATA ATCCAGCTCT GGATACGGAC 
GATGACCTGC CCACTTTTAC CTCTGCTATG CGCCTGCCTC CAGCCCCGGG GTCCACGGTG 
CCTGGCACAC CACCTCCCAG ATACAATACC TTGCGCTTGG ATAGAGCCTT TTCATCCCAG 
CTCACAGACA CTCAGTTGAC CAATGAGTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_058742.2
CDS	82..2034
Translation	MAPGEKIKAKIKKNLPVRGPQAPTIKDLMHWYCMNTNTHGCRRIVVSRGRLRRLLWIAFTLTAVALIIWQCALLVFSFYTVSVSIKVHFQKLDFPAVTICNINPYKYSAVSDLLTDLDSETKQALLSLYGVKESRKRREAGSMPSTLEGTPPRFFKLIPLLVFNENEKGKARDFFTGRKRKISGKIIHKASNVMHVHESKKLVGFQLCSNDTSDCATYTFSSGINAIQEWYKLHYMNIMAQVPLEKKINMSYSAKELLVTCFFDGMSCDARNFTLFHHPMYGNCYTFNNKENATILSTSMGGSEYGLQVILYINEDEYNPFLVSSTGAKVLIHQQNEYPFIEDVGMEIETAMSTSIGMHLTESFKLSEPYSQCTEDGSDVPVTNIYNAAYSLQICLYSCFQTKMVEKCGCAQYSQPLPPAANYCNYQQHPNWMYCYYQLYQAFVREELGCQSVCKQSCSFKEWTLTTSLAQWPSEASEKWLLNVLTWDQSQQINKKLNKTDLAKLLIFYKDLNQRSIMESPANSIEMLLSNFGGQLGLWMSCSVVCVIEIIEVFFIDFFSIIARRQWHKAKDWWARRQTPPSTETPSSRQGQDNPALDTDDDLPTFTSAMRLPPAPGSTVPGTPPPRYNTLRLDRAFSSQLTDTQLTNEL

Target ORF information:

RefSeq Version	NM_017046.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium channel epithelial 1 subunit gamma (Scnn1g), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017046.2

ORF Insert Sequence:

ATGGCGCCTG GAGAGAAGAT CAAAGCCAAA ATCAAAAAGA ATCTGCCGGT TCGAGGCCCC 
CAGGCACCAA CCATTAAGGA CCTGATGCAT TGGTACTGCA TGAACACCAA CACCCACGGC 
TGCCGCCGCA TCGTGGTGTC CCGAGGCCGC CTTCGGCGCC TGCTGTGGAT CGCGTTCACG 
CTAACTGCAG TGGCCCTCAT TATCTGGCAG TGCGCCCTCC TCGTCTTCTC TTTCTACACC 
GTCTCTGTCT CCATCAAAGT CCACTTCCAG AAACTGGATT TCCCTGCCGT CACTATCTGC 
AATATCAACC CTTACAAGTA CAGTGCTGTG AGTGACCTTC TGACTGACTT GGACAGTGAG 
ACAAAACAGG CCTTGCTGTC CTTGTATGGG GTCAAAGAAT CTCGGAAACG CCGGGAAGCA 
GGATCCATGC CGTCCACCTT GGAAGGCACA CCACCCAGAT TCTTCAAACT GATCCCACTG 
CTGGTCTTCA ATGAGAATGA GAAGGGAAAG GCCAGGGACT TCTTCACTGG CCGGAAGCGG 
AAAATCAGCG GGAAAATCAT TCACAAAGCT TCTAATGTCA TGCACGTTCA TGAGTCGAAG 
AAACTGGTGG GATTTCAATT GTGCTCAAAT GATACCTCTG ACTGCGCCAC CTACACCTTC 
AGCTCAGGAA TCAATGCCAT CCAGGAGTGG TACAAGCTTC ACTACATGAA TATCATGGCA 
CAGGTGCCTC TAGAGAAGAA AATCAACATG AGCTATTCTG CCAAAGAACT GCTGGTGACC 
TGCTTCTTCG ATGGGATGTC CTGTGATGCC AGGAACTTCA CGCTTTTCCA CCATCCAATG 
TATGGGAACT GCTACACATT TAACAACAAA GAAAATGCCA CCATCCTCAG CACCTCCATG 
GGAGGCAGTG AGTACGGGCT GCAAGTCATC TTATACATAA ACGAAGATGA ATACAACCCC 
TTCCTGGTGT CCTCCACTGG AGCCAAGGTG CTTATCCATC AGCAGAATGA ATACCCCTTC 
ATCGAAGACG TGGGGATGGA GATCGAGACA GCAATGTCCA CTTCCATAGG AATGCACCTG 
ACAGAATCCT TCAAGCTGAG CGAACCTTAC AGCCAGTGCA CAGAGGATGG CAGTGATGTG 
CCCGTCACAA ACATCTACAA CGCTGCCTAC TCCCTCCAGA TCTGCCTTTA TTCATGCTTC 
CAAACAAAGA TGGTGGAAAA ATGTGGCTGT GCCCAGTACA GCCAGCCTCT GCCTCCAGCA 
GCCAATTACT GCAACTACCA GCAACACCCC AACTGGATGT ATTGCTACTA CCAGTTGTAC 
CAGGCCTTTG TCCGGGAAGA ACTGGGCTGC CAATCAGTGT GCAAGCAATC CTGTAGCTTT 
AAGGAATGGA CACTGACCAC CAGCTTGGCA CAATGGCCGT CTGAGGCTTC CGAGAAATGG 
TTGCTGAATG TTCTCACCTG GGACCAAAGC CAGCAAATAA ACAAAAAGCT CAACAAGACT 
GACCTGGCCA AGCTCTTGAT ATTCTACAAA GACCTGAACC AAAGATCCAT CATGGAGAGC 
CCAGCCAACA GTATTGAGAT GCTCCTGTCC AACTTTGGTG GACAGCTCGG CCTGTGGATG 
AGCTGCTCAG TCGTCTGTGT CATTGAAATC ATTGAAGTCT TCTTCATTGA CTTCTTCTCT 
ATCATCGCCC GCCGTCAGTG GCACAAAGCC AAGGATTGGT GGGCCCGTAG GCAGACACCG 
CCCTCCACTG AGACTCCCTC CAGCCGGCAA GGCCAGGATA ATCCAGCTCT GGATACGGAC 
GATGACCTGC CCACTTTTAC CTCTGCTATG CGCCTGCCTC CAGCCCCGGG GTCCACGGTG 
CCTGGCACAC CACCTCCCAG ATACAATACC TTGCGCTTGG ATAGAGCCTT TTCATCCCAG 
CTCACAGACA CTCAGTTGAC CAATGAGTTG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Changes in plasma aldosterone and electrolytes levels, kidney epithelial sodium channel (ENaC) and blood pressure in normotensive WKY and hypertensive SHR rats following gonadectomy and chronic testosterone treatment.
Steroids128128-135(2017 Dec)
Loh SY,Giribabu N,Salleh N

Involvement of Epithelial Na
Scientific reports745996(2017 Apr)
Choi SK,Yeon SI,Kwon Y,Byeon S,Lee YH

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Scnn1g cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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