DLEC1 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005222552.3	XP_005222609.1	deleted in lung and esophageal cancer protein 1 isoform X1
XM_015459457.1	XP_015314943.1	deleted in lung and esophageal cancer protein 1 isoform X3
NM_001193198.1	NP_001180127.1	deleted in lung and esophageal cancer protein 1
XM_005222552.4	XP_005222609.2	deleted in lung and esophageal cancer protein 1 isoform X1
XM_005222554.3	XP_005222611.1	deleted in lung and esophageal cancer protein 1 isoform X2
XM_024983054.1	XP_024838822.1	deleted in lung and esophageal cancer protein 1 isoform X2
NM_001193198.2	NP_001180127.2	deleted in lung and esophageal cancer protein 1
XM_024983055.1	XP_024838823.1	deleted in lung and esophageal cancer protein 1 isoform X2
NM_001193198.3	NP_001180127.3	deleted in lung and esophageal cancer protein 1
XM_005222554.4	XP_005222611.2	deleted in lung and esophageal cancer protein 1 isoform X3
XM_015459457.2	XP_015314943.2	deleted in lung and esophageal cancer protein 1 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa229173	XM_024983054.1 Latest version!	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa229173	XM_024983055.1 Latest version!	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa229174	XM_005222554.4 Latest version!	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa229175	XM_015459457.2 Latest version!	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa94356	XM_015459457.1 Latest version!	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OBa216414	NM_001193198.2 Latest version!	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OBa238990	NM_001193198.3 Latest version!	Bos taurus DLEC1 cilia and flagella associated protein (DLEC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa229172	XM_005222552.4 Latest version!	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa01789	NM_001193198.1 Latest version!	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31377	XM_005222552.3 Latest version!	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa31379	XM_005222554.3 Latest version!	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

DLEC1 ( XM_024983054.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838822.1 Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa229173
Clone ID Related Accession (Same CDS sequence)	XM_024983054.1 , XM_024983055.1
Accession Version	XM_024983054.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	deleted in lung and esophageal cancer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCGCGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGGT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCATCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GAGTGGGTGG GGCTGGGAGC TTCGGGGAGC GTCCCCCAAG 
CAAGGCTGGG GAGGGGGGCG GTTGTCCAGC GCTGAGGCTG CTGTGTCTGC CAGGTGGTCC 
CTGCGCGGGG CAGCAGCACC ATCCACATCT CCTTCACACC CGTGGTGCTG GGCCCTGGCG 
TCCTGCACAA GGTGGAGTGT GTTGGCTATG CCCTGGGCTT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838822.1
CDS	1..4365
Translation	METGKRERQSAESGSYAFAGATWTPSPRLARIRSPSQSTWLSSLAYSESFHHGFGGRRRFVAQLSLPPLPEPQLLRLRPTSLRTQDISYLLTGVFRNLYTAEVIGEEVSSNLIKARGSEDARHEEFVDQLQQIRELYKQRLDEVEMLERHIIQARARALAEKERAMHQAKVQFLEPFIKMPPVKTIFRWCVDNELLRKHHLICPNDYYSDTVPFCSAPKGVSVPGYSKPTFSSEKRSIPKKELTKKPACRKMSSQSAEETDYTMDSLTWDSSFKAKLRSRAAIRKTGLPKNKNWMNHLRVPQRQLERLQLARMESRNRFLRNPRFFPPNTPLGGKSLLFPPKKPARVGAFQDGELEESCADTPVFLAKPSIGFFTDYEIGPVYEMVISLQNTTSTSRHLRVLPPSTPYFALGLGMFPGEGGMVAPGMTCQYIVQFFPDCLGDFDDFILVETQSAHTLLIPLQARRPPPVLTLSPVLDCGYCLIGGMKITRFICKNVGFSVGKFCIMPKKSWPPPSFRAVVTIGFVEQPPFGILPSVFELVPGQAVFVEVLFLPTSLGKAEQTFVIVCDNCQIKELVTTGVGQLVALDLIYISGEKSQPEPGELTDLTAQYFIRFEPENLQSTASKLLIIRNATHVELAFHWQIMKPNLQSLMPGETYSTDSIKYHPDRETAFCITPKMGVLSPHTDHEFILSFSPQEPRDFHSVLQMVLEEVPEPVSLDLENSKDPSYCMDDVIVLEIEVKGSVEPFQVLLEPYALIIPGENYIGINVKKNFKMWNNSKSTIRYTWGKISDCHIIEVEPCTGTIEPSEVEDFELNFTGGVPGPTSQNLLCEIKDSPTPVVLHIEAAFKGPALVIDVSALQFGLLRLGQKATKFIQIRNLSQLPATWAMKESQVSLQERQEGVSPFDIEPSSGQVQPLGECRVSITLEAQHCQRLQTVLELEVVDGTWSHLPVYAEVQEPYVYLQSSQVEVPNLYLGVPTKMAITLINGTLLPTRFHWGKLLGLQADVCTVKVSPRRGTLGPSEERQLSLELTAHTLEAVTDLALPCDVSGMREPLVLGISGKPRGLQVAIGISKEDSDDSAPSTELWPGHPETLHLDFGSAVPLWTRVNRRLFLTNCSPIQTPFTLEFEYFGSPVNSLSQKPSPPDMPPALLKTARMRELLAKREQWEFMESMLSHGKGAAFFPHLSQGVLGAHQKLSIDLTACANMWGEYWDSLICTVGGLPPAVIPVRMAVVGCPISSQRTTYYTTDQTQREPIIRFGTRVSGGDPVTRILRLNNSSPCDIRLDWETYTPEDREDRLLELLVFYGPPFPLRDHAGNDLLYPETPESSGSFWSPSPSESESSREATPSAEGSSSAGAPQKIISVILRGHEGVPSDHLFSISPKQVVSGWGWELRGASPKQGWGGGRLSSAEAAVSARWSLRGAAAPSTSPSHPWCWALASCTRWSVLAMPWAS

Target ORF information:

RefSeq Version	XM_024983054.1
Organism	Bos taurus(cattle)
Definition	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983054.1

ORF Insert Sequence:

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCGCGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGGT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCATCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GAGTGGGTGG GGCTGGGAGC TTCGGGGAGC GTCCCCCAAG 
CAAGGCTGGG GAGGGGGGCG GTTGTCCAGC GCTGAGGCTG CTGTGTCTGC CAGGTGGTCC 
CTGCGCGGGG CAGCAGCACC ATCCACATCT CCTTCACACC CGTGGTGCTG GGCCCTGGCG 
TCCTGCACAA GGTGGAGTGT GTTGGCTATG CCCTGGGCTT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLEC1 ( XM_024983055.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024838823.1 Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa229173
Clone ID Related Accession (Same CDS sequence)	XM_024983054.1 , XM_024983055.1
Accession Version	XM_024983055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	deleted in lung and esophageal cancer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCGCGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGGT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCATCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GAGTGGGTGG GGCTGGGAGC TTCGGGGAGC GTCCCCCAAG 
CAAGGCTGGG GAGGGGGGCG GTTGTCCAGC GCTGAGGCTG CTGTGTCTGC CAGGTGGTCC 
CTGCGCGGGG CAGCAGCACC ATCCACATCT CCTTCACACC CGTGGTGCTG GGCCCTGGCG 
TCCTGCACAA GGTGGAGTGT GTTGGCTATG CCCTGGGCTT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024838823.1
CDS	1..4365
Translation	METGKRERQSAESGSYAFAGATWTPSPRLARIRSPSQSTWLSSLAYSESFHHGFGGRRRFVAQLSLPPLPEPQLLRLRPTSLRTQDISYLLTGVFRNLYTAEVIGEEVSSNLIKARGSEDARHEEFVDQLQQIRELYKQRLDEVEMLERHIIQARARALAEKERAMHQAKVQFLEPFIKMPPVKTIFRWCVDNELLRKHHLICPNDYYSDTVPFCSAPKGVSVPGYSKPTFSSEKRSIPKKELTKKPACRKMSSQSAEETDYTMDSLTWDSSFKAKLRSRAAIRKTGLPKNKNWMNHLRVPQRQLERLQLARMESRNRFLRNPRFFPPNTPLGGKSLLFPPKKPARVGAFQDGELEESCADTPVFLAKPSIGFFTDYEIGPVYEMVISLQNTTSTSRHLRVLPPSTPYFALGLGMFPGEGGMVAPGMTCQYIVQFFPDCLGDFDDFILVETQSAHTLLIPLQARRPPPVLTLSPVLDCGYCLIGGMKITRFICKNVGFSVGKFCIMPKKSWPPPSFRAVVTIGFVEQPPFGILPSVFELVPGQAVFVEVLFLPTSLGKAEQTFVIVCDNCQIKELVTTGVGQLVALDLIYISGEKSQPEPGELTDLTAQYFIRFEPENLQSTASKLLIIRNATHVELAFHWQIMKPNLQSLMPGETYSTDSIKYHPDRETAFCITPKMGVLSPHTDHEFILSFSPQEPRDFHSVLQMVLEEVPEPVSLDLENSKDPSYCMDDVIVLEIEVKGSVEPFQVLLEPYALIIPGENYIGINVKKNFKMWNNSKSTIRYTWGKISDCHIIEVEPCTGTIEPSEVEDFELNFTGGVPGPTSQNLLCEIKDSPTPVVLHIEAAFKGPALVIDVSALQFGLLRLGQKATKFIQIRNLSQLPATWAMKESQVSLQERQEGVSPFDIEPSSGQVQPLGECRVSITLEAQHCQRLQTVLELEVVDGTWSHLPVYAEVQEPYVYLQSSQVEVPNLYLGVPTKMAITLINGTLLPTRFHWGKLLGLQADVCTVKVSPRRGTLGPSEERQLSLELTAHTLEAVTDLALPCDVSGMREPLVLGISGKPRGLQVAIGISKEDSDDSAPSTELWPGHPETLHLDFGSAVPLWTRVNRRLFLTNCSPIQTPFTLEFEYFGSPVNSLSQKPSPPDMPPALLKTARMRELLAKREQWEFMESMLSHGKGAAFFPHLSQGVLGAHQKLSIDLTACANMWGEYWDSLICTVGGLPPAVIPVRMAVVGCPISSQRTTYYTTDQTQREPIIRFGTRVSGGDPVTRILRLNNSSPCDIRLDWETYTPEDREDRLLELLVFYGPPFPLRDHAGNDLLYPETPESSGSFWSPSPSESESSREATPSAEGSSSAGAPQKIISVILRGHEGVPSDHLFSISPKQVVSGWGWELRGASPKQGWGGGRLSSAEAAVSARWSLRGAAAPSTSPSHPWCWALASCTRWSVLAMPWAS

Target ORF information:

RefSeq Version	XM_024983055.1
Organism	Bos taurus(cattle)
Definition	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024983055.1

ORF Insert Sequence:

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCGCGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGGT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCATCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GAGTGGGTGG GGCTGGGAGC TTCGGGGAGC GTCCCCCAAG 
CAAGGCTGGG GAGGGGGGCG GTTGTCCAGC GCTGAGGCTG CTGTGTCTGC CAGGTGGTCC 
CTGCGCGGGG CAGCAGCACC ATCCACATCT CCTTCACACC CGTGGTGCTG GGCCCTGGCG 
TCCTGCACAA GGTGGAGTGT GTTGGCTATG CCCTGGGCTT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLEC1 ( XM_005222554.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222611.2 Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X5, mRNA.

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CloneID	OBa229174
Clone ID Related Accession (Same CDS sequence)	XM_005222554.4
Accession Version	XM_005222554.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	deleted in lung and esophageal cancer protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222554.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCGCGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGGT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCATCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GGTCCCTGCG CGGGGCAGCA GCACCATCCA CATCTCCTTC 
ACACCCGTGG TGCTGGGCCC TGGCGTCCTG CACAAGGTGG AGTGTGTTGG CTATGCCCTG 
GGCTTCTTGA GCTTGGACGA TGAGGACTCC AGTGATGGGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222611.2
CDS	1..4305
Translation	METGKRERQSAESGSYAFAGATWTPSPRLARIRSPSQSTWLSSLAYSESFHHGFGGRRRFVAQLSLPPLPEPQLLRLRPTSLRTQDISYLLTGVFRNLYTAEVIGEEVSSNLIKARGSEDARHEEFVDQLQQIRELYKQRLDEVEMLERHIIQARARALAEKERAMHQAKVQFLEPFIKMPPVKTIFRWCVDNELLRKHHLICPNDYYSDTVPFCSAPKGVSVPGYSKPTFSSEKRSIPKKELTKKPACRKMSSQSAEETDYTMDSLTWDSSFKAKLRSRAAIRKTGLPKNKNWMNHLRVPQRQLERLQLARMESRNRFLRNPRFFPPNTPLGGKSLLFPPKKPARVGAFQDGELEESCADTPVFLAKPSIGFFTDYEIGPVYEMVISLQNTTSTSRHLRVLPPSTPYFALGLGMFPGEGGMVAPGMTCQYIVQFFPDCLGDFDDFILVETQSAHTLLIPLQARRPPPVLTLSPVLDCGYCLIGGMKITRFICKNVGFSVGKFCIMPKKSWPPPSFRAVVTIGFVEQPPFGILPSVFELVPGQAVFVEVLFLPTSLGKAEQTFVIVCDNCQIKELVTTGVGQLVALDLIYISGEKSQPEPGELTDLTAQYFIRFEPENLQSTASKLLIIRNATHVELAFHWQIMKPNLQSLMPGETYSTDSIKYHPDRETAFCITPKMGVLSPHTDHEFILSFSPQEPRDFHSVLQMVLEEVPEPVSLDLENSKDPSYCMDDVIVLEIEVKGSVEPFQVLLEPYALIIPGENYIGINVKKNFKMWNNSKSTIRYTWGKISDCHIIEVEPCTGTIEPSEVEDFELNFTGGVPGPTSQNLLCEIKDSPTPVVLHIEAAFKGPALVIDVSALQFGLLRLGQKATKFIQIRNLSQLPATWAMKESQVSLQERQEGVSPFDIEPSSGQVQPLGECRVSITLEAQHCQRLQTVLELEVVDGTWSHLPVYAEVQEPYVYLQSSQVEVPNLYLGVPTKMAITLINGTLLPTRFHWGKLLGLQADVCTVKVSPRRGTLGPSEERQLSLELTAHTLEAVTDLALPCDVSGMREPLVLGISGKPRGLQVAIGISKEDSDDSAPSTELWPGHPETLHLDFGSAVPLWTRVNRRLFLTNCSPIQTPFTLEFEYFGSPVNSLSQKPSPPDMPPALLKTARMRELLAKREQWEFMESMLSHGKGAAFFPHLSQGVLGAHQKLSIDLTACANMWGEYWDSLICTVGGLPPAVIPVRMAVVGCPISSQRTTYYTTDQTQREPIIRFGTRVSGGDPVTRILRLNNSSPCDIRLDWETYTPEDREDRLLELLVFYGPPFPLRDHAGNDLLYPETPESSGSFWSPSPSESESSREATPSAEGSSSAGAPQKIISVILRGHEGVPSDHLFSISPKQVVVPARGSSTIHISFTPVVLGPGVLHKVECVGYALGFLSLDDEDSSDGA

Target ORF information:

RefSeq Version	XM_005222554.4
Organism	Bos taurus(cattle)
Definition	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222554.4

ORF Insert Sequence:

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCGCGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGGT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCATCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GGTCCCTGCG CGGGGCAGCA GCACCATCCA CATCTCCTTC 
ACACCCGTGG TGCTGGGCCC TGGCGTCCTG CACAAGGTGG AGTGTGTTGG CTATGCCCTG 
GGCTTCTTGA GCTTGGACGA TGAGGACTCC AGTGATGGGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLEC1 ( XM_015459457.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314943.2 Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X7, mRNA.

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CloneID	OBa229175
Clone ID Related Accession (Same CDS sequence)	XM_015459457.2
Accession Version	XM_015459457.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	deleted in lung and esophageal cancer protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015459457.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCGCGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGGT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCATCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GGTCCCTGCG CGGGGCAGCA GCACCATCCA CATCTCCTTC 
ACACCCGTGG TGCTGGGCCC TGGCGTCCTG CACAAGGTGG AGTGTGTTGG CTATGCCCTG 
GGCTTCTTGA GCTTGGACGA TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314943.2
CDS	1..4287
Translation	METGKRERQSAESGSYAFAGATWTPSPRLARIRSPSQSTWLSSLAYSESFHHGFGGRRRFVAQLSLPPLPEPQLLRLRPTSLRTQDISYLLTGVFRNLYTAEVIGEEVSSNLIKARGSEDARHEEFVDQLQQIRELYKQRLDEVEMLERHIIQARARALAEKERAMHQAKVQFLEPFIKMPPVKTIFRWCVDNELLRKHHLICPNDYYSDTVPFCSAPKGVSVPGYSKPTFSSEKRSIPKKELTKKPACRKMSSQSAEETDYTMDSLTWDSSFKAKLRSRAAIRKTGLPKNKNWMNHLRVPQRQLERLQLARMESRNRFLRNPRFFPPNTPLGGKSLLFPPKKPARVGAFQDGELEESCADTPVFLAKPSIGFFTDYEIGPVYEMVISLQNTTSTSRHLRVLPPSTPYFALGLGMFPGEGGMVAPGMTCQYIVQFFPDCLGDFDDFILVETQSAHTLLIPLQARRPPPVLTLSPVLDCGYCLIGGMKITRFICKNVGFSVGKFCIMPKKSWPPPSFRAVVTIGFVEQPPFGILPSVFELVPGQAVFVEVLFLPTSLGKAEQTFVIVCDNCQIKELVTTGVGQLVALDLIYISGEKSQPEPGELTDLTAQYFIRFEPENLQSTASKLLIIRNATHVELAFHWQIMKPNLQSLMPGETYSTDSIKYHPDRETAFCITPKMGVLSPHTDHEFILSFSPQEPRDFHSVLQMVLEEVPEPVSLDLENSKDPSYCMDDVIVLEIEVKGSVEPFQVLLEPYALIIPGENYIGINVKKNFKMWNNSKSTIRYTWGKISDCHIIEVEPCTGTIEPSEVEDFELNFTGGVPGPTSQNLLCEIKDSPTPVVLHIEAAFKGPALVIDVSALQFGLLRLGQKATKFIQIRNLSQLPATWAMKESQVSLQERQEGVSPFDIEPSSGQVQPLGECRVSITLEAQHCQRLQTVLELEVVDGTWSHLPVYAEVQEPYVYLQSSQVEVPNLYLGVPTKMAITLINGTLLPTRFHWGKLLGLQADVCTVKVSPRRGTLGPSEERQLSLELTAHTLEAVTDLALPCDVSGMREPLVLGISGKPRGLQVAIGISKEDSDDSAPSTELWPGHPETLHLDFGSAVPLWTRVNRRLFLTNCSPIQTPFTLEFEYFGSPVNSLSQKPSPPDMPPALLKTARMRELLAKREQWEFMESMLSHGKGAAFFPHLSQGVLGAHQKLSIDLTACANMWGEYWDSLICTVGGLPPAVIPVRMAVVGCPISSQRTTYYTTDQTQREPIIRFGTRVSGGDPVTRILRLNNSSPCDIRLDWETYTPEDREDRLLELLVFYGPPFPLRDHAGNDLLYPETPESSGSFWSPSPSESESSREATPSAEGSSSAGAPQKIISVILRGHEGVPSDHLFSISPKQVVVPARGSSTIHISFTPVVLGPGVLHKVECVGYALGFLSLDDE

Target ORF information:

RefSeq Version	XM_015459457.2
Organism	Bos taurus(cattle)
Definition	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459457.2

ORF Insert Sequence:

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCGCGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGGT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCATCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GGTCCCTGCG CGGGGCAGCA GCACCATCCA CATCTCCTTC 
ACACCCGTGG TGCTGGGCCC TGGCGTCCTG CACAAGGTGG AGTGTGTTGG CTATGCCCTG 
GGCTTCTTGA GCTTGGACGA TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLEC1 ( XM_015459457.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015314943.1 Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X3, mRNA.

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CloneID	OBa94356
Clone ID Related Accession (Same CDS sequence)	XM_015459457.1
Accession Version	XM_015459457.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4287bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	deleted in lung and esophageal cancer protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104540.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCACGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGAT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCGTCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GGTCCCTGCG CGGGGCAGCA GCACCATCCA CATCTCCTTC 
ACACCCGTGG TGCTGGGCCC TGGCGTCCTG CACAAGGTGG AGTGTGTTGG CTATGCCCTG 
GGCTTCTTGA GCTTGGACGA TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015314943.1
CDS	181..4467
Translation	METGKRERQSAESGSYAFAGATWTPSPRLARIRSPSQSTWLSSLAYSESFHHGFGGRRRFVAQLSLPPLPEPQLLRLRPTSLRTQDISYLLTGVFRNLYTAEVIGEEVSSNLIKARGSEDARHEEFVDQLQQIRELYKQRLDEVEMLERHIIQARARALAEKERAMHQAKVQFLEPFIKMPPVKTIFRWCVDNELLRKHHLICPNDYYSDTVPFCSAPKGVSVPGYSKPTFSSEKRSIPKKELTKKPACRKMSSQSAEETDYTMDSLTWDSSFKAKLRSRAAIRKTGLPKNKNWMNHLRVPQRQLERLQLARMESRNRFLRNPRFFPPNTPLGGKSLLFPPKKPARIGAFQDGELEESCADTPVFLAKPSIGFFTDYEIGPVYEMVISLQNTTSTSRHLRVLPPSTPYFALGLGMFPGEGGMVAPGMTCQYIVQFFPDCLGDFDDFILVETQSAHTLLIPLQARRPPPVLTLSPVLDCGYCLIGGMKITRFICKNVGFSVGKFCIMPKKSWPPPSFRAVVTIGFVEQPPFGILPSVFELVPGQAVFVEVLFLPTSLGKAEQTFVIVCDNCQIKELVTTGVGQLVALDLIYISGEKSQPEPGELTDLTAQYFIRFEPENLQSTASKLLIIRNATHVELAFHWQIMKPNLQSLMPGETYSTDSIKYHPDRETAFCITPKMGVLSPHTDHEFILSFSPQEPRDFHSVLQMVLEEVPEPVSLDLENSKDPSYCMDDVIVLEIEVKGSVEPFQVLLEPYALIIPGENYIGINVKKNFKMWNNSKSTIRYTWGKISDCHIIEVEPCTGTIEPSEVEDFELNFTGGVPGPTSQNLLCEIKDSPTPVVLHIEAAFKGPALVIDVSALQFGLLRLGQKATKFIQIRNLSQLPATWAMKESQVSLQERQEGVSPFDIEPSSGQVQPLGECRVSITLEAQHCQRLQTVLELEVVDGTWSHLPVYAEVQEPYVYLQSSQVEVPNLYLGVPTKMAITLINGTLLPTRFHWGKLLGLQADVCTVKVSPRRGTLGPSEERQLSLELTAHTLEAVTDLALPCDVSGMREPLVLGISGKPRGLQVAIGVSKEDSDDSAPSTELWPGHPETLHLDFGSAVPLWTRVNRRLFLTNCSPIQTPFTLEFEYFGSPVNSLSQKPSPPDMPPALLKTARMRELLAKREQWEFMESMLSHGKGAAFFPHLSQGVLGAHQKLSIDLTACANMWGEYWDSLICTVGGLPPAVIPVRMAVVGCPISSQRTTYYTTDQTQREPIIRFGTRVSGGDPVTRILRLNNSSPCDIRLDWETYTPEDREDRLLELLVFYGPPFPLRDHAGNDLLYPETPESSGSFWSPSPSESESSREATPSAEGSSSAGAPQKIISVILRGHEGVPSDHLFSISPKQVVVPARGSSTIHISFTPVVLGPGVLHKVECVGYALGFLSLDDE

Target ORF information:

RefSeq Version	XM_015459457.1
Organism	Bos taurus(cattle)
Definition	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015459457.1

ORF Insert Sequence:

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCACGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGAT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCGTCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GGTCCCTGCG CGGGGCAGCA GCACCATCCA CATCTCCTTC 
ACACCCGTGG TGCTGGGCCC TGGCGTCCTG CACAAGGTGG AGTGTGTTGG CTATGCCCTG 
GGCTTCTTGA GCTTGGACGA TGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLEC1 ( NM_001193198.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180127.2 Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), mRNA.

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CloneID	OBa216414
Clone ID Related Accession (Same CDS sequence)	NM_001193198.2
Accession Version	NM_001193198.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-22
Organism	Bos taurus(cattle)
Product	deleted in lung and esophageal cancer protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from DAAA02053645.1. On Jun 30, 2017 this sequence version replaced NM_001193198.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN04019650 [ECO:0000348] ##Evidence-Data-END##

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCACGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGAT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCGTCTCTA AGGAGGACAG TGACGACAGC 
ACAGAGCTGT GGCCGGGCCA CCCCGAGACG CTCCACCTGG ACTTCGGCTC GGCAGTGCCA 
CTGTGGACCC GTGTGAACCG CCGGCTCTTC CTGACCAACT GCTCCCCGAT ACAGACCCCT 
TTCACCCTCG AGTTCGAGTA CTTTGGGAGC CCCGTGAACA GCCTGAGCCA GAAACCCAGC 
CCCCCCGACA TGCCTCCCGC CCTGCTGAAG ACGGCACGGA TGCGGGAGCT CTTGGCCAAG 
CGCGAGCAGT GGGAGTTTAT GGAAAGCATG CTGTCCCATG GGAAAGGAGC AGCCTTCTTC 
CCCCACCTCT CCCAGGGCGT GCTGGGCGCC CACCAGAAGC TCAGCATCGA CCTCACTGCC 
TGTGCCAACA TGTGGGGGGA GTACTGGGAT AGTCTCATCT GCACGGTGGG AGGCCTGCCT 
CCAGCAGTCA TCCCAGTGCG CATGGCGGTG GTGGGCTGCC CCATCAGCTC CCAGAGGACC 
ACCTACTACA CCACTGACCA GACCCAGAGG GAGCCCATCA TCAGGTTCGG CACCCGGGTC 
TCTGGAGGAG ACCCAGTCAC CCGGATCCTT CGCCTGAATA ATTCCAGCCC CTGTGACATC 
CGCCTGGACT GGGAGACCTA CACTCCAGAG GACAGGGAGG ACCGGCTGCT GGAGCTGCTG 
GTGTTCTATG GGCCGCCCTT CCCGCTACGT GACCACGCTG GGAATGACCT CCTGTACCCC 
GAGACCCCGG AGAGCAGCGG CTCCTTCTGG TCCCCAAGTC CCTCTGAGTC TGAGTCCAGC 
CGTGAAGCCA CCCCATCTGC CGAGGGCAGC TCCAGTGCTG GCGCCCCCCA GAAGATCATC 
TCAGTCATCC TGCGAGGGCA TGAGGGGGTG CCCTCTGACC ACCTGTTCTC CATCAGCCCC 
AAGCAGGTGG TGGTCCCTGC GCGGGGCAGC AGCACCATCC ACATCTCCTT CACACCCGTG 
GTGCTGGGCC CTGGCGTCCT GCACAAGGTG GAGTGTGTTG GCTATGCCCT GGGCTTCTTG 
AGCTTGGACG ATGAGGTGGA GAGGGAGCTT CCAGGCCGGA GGCGCCGCCT GCAGGACTTT 
GCCATGGGAC CCATGAGACT GGACCTGCAT GGCTACGTCA GGCCGGCGCA GCTGAGCGTG 
GACTGGGACT ACGGTGGCAG CATGGTGTTC CGGCAGCAGG CCAGCGACCT CATCCCTGAG 
CAGCCCTGCG CCGGGGTGCT GAGTGAGCTG GTGACCACCC GCCACCTGAA GCTGACCAAC 
ACCACGGAAA TCCCTCACTA CTTCTGGCTC CTGGTCTCCA AGCCCTTCTC CATTTCTCAA 
GACGGGGCGA GCCGCAGCCA CCGGGCCCTT GGCTGGAAGC AGGAGGGTGA GGAGATAGCA 
GCGAGGGGCA AGCAGCTGGT GCTCCACCCA CAGGAGAACA TGCTGGTGAA CGTGTCCTTC 
TCGCTCTCCC TGGAGCTGCT GTCCTATCAG AAGCTCCCGG CTGACCAGAT GCTGCCCGGA 
GTGGACATTC AGCAGAGCCC AAGTGGAGAG AAGCAGATGG TGTTCACACA GAACCTGCTG 
CTGGAATACA GCAACCAGAC CTCACAGGTG GTGCCCCTGC GGGCCGTGGT GGCCGTGCCC 
GAGCTGCAGC TCTCCACCAG CTGGGTGGAC TTCGGCCCCT GCTTCGTGAA CCAGGGCCGG 
GCCCGGGAGG TCTACCTCAT GAACCTGAGT GGCTGCCGAA GCTATTGGGC TGTGCTGACA 
GGCCAGCAGG AGCCAGACAG GGACCCCGTG GCCTTCAAGG TGTCCCCAAG CAGTGGGCTG 
CTGGAAGCAC GACCAGTCAA CGCACCCCCC ACCTCTGTCA CCCTCCAGGT TTCCTTCACC 
GCCAGGAGCT GTGAGCTGTA CGAGTCCACG CTGGTGGTGG AAGGCGTGCT CGGGGAGAAG 
CCGTGTACCC TGCGGCTCCG GGGCCGCGGC TCATATGACG AGAGACATGC TTCACCCCAG 
CACCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180127.2
CDS	1..5232
Translation	METGKRERQSAESGSYAFAGATWTPSPRLARIRSPSQSTWLSSLAYSESFHHGFGGRRRFVAQLSLPPLPEPQLLRLRPTSLRTQDISYLLTGVFRNLYTAEVIGEEVSSNLIKARGSEDARHEEFVDQLQQIRELYKQRLDEVEMLERHIIQARARALAEKERAMHQAKVQFLEPFIKMPPVKTIFRWCVDNELLRKHHLICPNDYYSDTVPFCSAPKGVSVPGYSKPTFSSEKRSIPKKELTKKPACRKMSSQSAEETDYTMDSLTWDSSFKAKLRSRAAIRKTGLPKNKNWMNHLRVPQRQLERLQLARMESRNRFLRNPRFFPPNTPLGGKSLLFPPKKPARIGAFQDGELEESCADTPVFLAKPSIGFFTDYEIGPVYEMVISLQNTTSTSRHLRVLPPSTPYFALGLGMFPGEGGMVAPGMTCQYIVQFFPDCLGDFDDFILVETQSAHTLLIPLQARRPPPVLTLSPVLDCGYCLIGGMKITRFICKNVGFSVGKFCIMPKKSWPPPSFRAVVTIGFVEQPPFGILPSVFELVPGQAVFVEVLFLPTSLGKAEQTFVIVCDNCQIKELVTTGVGQLVALDLIYISGEKSQPEPGELTDLTAQYFIRFEPENLQSTASKLLIIRNATHVELAFHWQIMKPNLQSLMPGETYSTDSIKYHPDRETAFCITPKMGVLSPHTDHEFILSFSPQEPRDFHSVLQMVLEEVPEPVSLDLENSKDPSYCMDDVIVLEIEVKGSVEPFQVLLEPYALIIPGENYIGINVKKNFKMWNNSKSTIRYTWGKISDCHIIEVEPCTGTIEPSEVEDFELNFTGGVPGPTSQNLLCEIKDSPTPVVLHIEAAFKGPALVIDVSALQFGLLRLGQKATKFIQIRNLSQLPATWAMKESQVSLQERQEGVSPFDIEPSSGQVQPLGECRVSITLEAQHCQRLQTVLELEVVDGTWSHLPVYAEVQEPYVYLQSSQVEVPNLYLGVPTKMAITLINGTLLPTRFHWGKLLGLQADVCTVKVSPRRGTLGPSEERQLSLELTAHTLEAVTDLALPCDVSGMREPLVLGISGKPRGLQVAIGVSKEDSDDSTELWPGHPETLHLDFGSAVPLWTRVNRRLFLTNCSPIQTPFTLEFEYFGSPVNSLSQKPSPPDMPPALLKTARMRELLAKREQWEFMESMLSHGKGAAFFPHLSQGVLGAHQKLSIDLTACANMWGEYWDSLICTVGGLPPAVIPVRMAVVGCPISSQRTTYYTTDQTQREPIIRFGTRVSGGDPVTRILRLNNSSPCDIRLDWETYTPEDREDRLLELLVFYGPPFPLRDHAGNDLLYPETPESSGSFWSPSPSESESSREATPSAEGSSSAGAPQKIISVILRGHEGVPSDHLFSISPKQVVVPARGSSTIHISFTPVVLGPGVLHKVECVGYALGFLSLDDEVERELPGRRRRLQDFAMGPMRLDLHGYVRPAQLSVDWDYGGSMVFRQQASDLIPEQPCAGVLSELVTTRHLKLTNTTEIPHYFWLLVSKPFSISQDGASRSHRALGWKQEGEEIAARGKQLVLHPQENMLVNVSFSLSLELLSYQKLPADQMLPGVDIQQSPSGEKQMVFTQNLLLEYSNQTSQVVPLRAVVAVPELQLSTSWVDFGPCFVNQGRAREVYLMNLSGCRSYWAVLTGQQEPDRDPVAFKVSPSSGLLEARPVNAPPTSVTLQVSFTARSCELYESTLVVEGVLGEKPCTLRLRGRGSYDERHASPQHQL

Target ORF information:

RefSeq Version	NM_001193198.2
Organism	Bos taurus(cattle)
Definition	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193198.2

ORF Insert Sequence:

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCACGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGAT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCGTCTCTA AGGAGGACAG TGACGACAGC 
ACAGAGCTGT GGCCGGGCCA CCCCGAGACG CTCCACCTGG ACTTCGGCTC GGCAGTGCCA 
CTGTGGACCC GTGTGAACCG CCGGCTCTTC CTGACCAACT GCTCCCCGAT ACAGACCCCT 
TTCACCCTCG AGTTCGAGTA CTTTGGGAGC CCCGTGAACA GCCTGAGCCA GAAACCCAGC 
CCCCCCGACA TGCCTCCCGC CCTGCTGAAG ACGGCACGGA TGCGGGAGCT CTTGGCCAAG 
CGCGAGCAGT GGGAGTTTAT GGAAAGCATG CTGTCCCATG GGAAAGGAGC AGCCTTCTTC 
CCCCACCTCT CCCAGGGCGT GCTGGGCGCC CACCAGAAGC TCAGCATCGA CCTCACTGCC 
TGTGCCAACA TGTGGGGGGA GTACTGGGAT AGTCTCATCT GCACGGTGGG AGGCCTGCCT 
CCAGCAGTCA TCCCAGTGCG CATGGCGGTG GTGGGCTGCC CCATCAGCTC CCAGAGGACC 
ACCTACTACA CCACTGACCA GACCCAGAGG GAGCCCATCA TCAGGTTCGG CACCCGGGTC 
TCTGGAGGAG ACCCAGTCAC CCGGATCCTT CGCCTGAATA ATTCCAGCCC CTGTGACATC 
CGCCTGGACT GGGAGACCTA CACTCCAGAG GACAGGGAGG ACCGGCTGCT GGAGCTGCTG 
GTGTTCTATG GGCCGCCCTT CCCGCTACGT GACCACGCTG GGAATGACCT CCTGTACCCC 
GAGACCCCGG AGAGCAGCGG CTCCTTCTGG TCCCCAAGTC CCTCTGAGTC TGAGTCCAGC 
CGTGAAGCCA CCCCATCTGC CGAGGGCAGC TCCAGTGCTG GCGCCCCCCA GAAGATCATC 
TCAGTCATCC TGCGAGGGCA TGAGGGGGTG CCCTCTGACC ACCTGTTCTC CATCAGCCCC 
AAGCAGGTGG TGGTCCCTGC GCGGGGCAGC AGCACCATCC ACATCTCCTT CACACCCGTG 
GTGCTGGGCC CTGGCGTCCT GCACAAGGTG GAGTGTGTTG GCTATGCCCT GGGCTTCTTG 
AGCTTGGACG ATGAGGTGGA GAGGGAGCTT CCAGGCCGGA GGCGCCGCCT GCAGGACTTT 
GCCATGGGAC CCATGAGACT GGACCTGCAT GGCTACGTCA GGCCGGCGCA GCTGAGCGTG 
GACTGGGACT ACGGTGGCAG CATGGTGTTC CGGCAGCAGG CCAGCGACCT CATCCCTGAG 
CAGCCCTGCG CCGGGGTGCT GAGTGAGCTG GTGACCACCC GCCACCTGAA GCTGACCAAC 
ACCACGGAAA TCCCTCACTA CTTCTGGCTC CTGGTCTCCA AGCCCTTCTC CATTTCTCAA 
GACGGGGCGA GCCGCAGCCA CCGGGCCCTT GGCTGGAAGC AGGAGGGTGA GGAGATAGCA 
GCGAGGGGCA AGCAGCTGGT GCTCCACCCA CAGGAGAACA TGCTGGTGAA CGTGTCCTTC 
TCGCTCTCCC TGGAGCTGCT GTCCTATCAG AAGCTCCCGG CTGACCAGAT GCTGCCCGGA 
GTGGACATTC AGCAGAGCCC AAGTGGAGAG AAGCAGATGG TGTTCACACA GAACCTGCTG 
CTGGAATACA GCAACCAGAC CTCACAGGTG GTGCCCCTGC GGGCCGTGGT GGCCGTGCCC 
GAGCTGCAGC TCTCCACCAG CTGGGTGGAC TTCGGCCCCT GCTTCGTGAA CCAGGGCCGG 
GCCCGGGAGG TCTACCTCAT GAACCTGAGT GGCTGCCGAA GCTATTGGGC TGTGCTGACA 
GGCCAGCAGG AGCCAGACAG GGACCCCGTG GCCTTCAAGG TGTCCCCAAG CAGTGGGCTG 
CTGGAAGCAC GACCAGTCAA CGCACCCCCC ACCTCTGTCA CCCTCCAGGT TTCCTTCACC 
GCCAGGAGCT GTGAGCTGTA CGAGTCCACG CTGGTGGTGG AAGGCGTGCT CGGGGAGAAG 
CCGTGTACCC TGCGGCTCCG GGGCCGCGGC TCATATGACG AGAGACATGC TTCACCCCAG 
CACCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLEC1 ( NM_001193198.3 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180127.3 Bos taurus DLEC1 cilia and flagella associated protein (DLEC1), mRNA.

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CloneID	OBa238990
Clone ID Related Accession (Same CDS sequence)	NM_001193198.3
Accession Version	NM_001193198.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-24
Organism	Bos taurus(cattle)
Product	deleted in lung and esophageal cancer protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000022.1. On May 29, 2018 this sequence version replaced NM_001193198.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145419, SAMN03145422 [ECO:0000350] ##Evidence-Data-END##

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCGCGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGGT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCATCTCTA AGGAGGACAG TGACGACAGC 
ACAGAGCTGT GGCCGGGCCA CCCCGAGACG CTCCACCTGG ACTTCGGCTC GGCAGTGCCA 
CTGTGGACCC GTGTGAACCG CCGGCTCTTC CTGACCAACT GCTCCCCGAT ACAGACCCCT 
TTCACCCTCG AGTTCGAGTA CTTTGGGAGC CCCGTGAACA GCCTGAGCCA GAAACCCAGC 
CCCCCCGACA TGCCTCCCGC CCTGCTGAAG ACGGCACGGA TGCGGGAGCT CTTGGCCAAG 
CGCGAGCAGT GGGAGTTTAT GGAAAGCATG CTGTCCCATG GGAAAGGAGC AGCCTTCTTC 
CCCCACCTCT CCCAGGGCGT GCTGGGCGCC CACCAGAAGC TCAGCATCGA CCTCACTGCC 
TGTGCCAACA TGTGGGGGGA GTACTGGGAT AGTCTCATCT GCACGGTGGG AGGCCTGCCT 
CCAGCAGTCA TCCCAGTGCG CATGGCGGTG GTGGGCTGCC CCATCAGCTC CCAGAGGACC 
ACCTACTACA CCACTGACCA GACCCAGAGG GAGCCCATCA TCAGGTTCGG CACCCGGGTC 
TCTGGAGGAG ACCCAGTCAC CCGGATCCTT CGCCTGAATA ATTCCAGCCC CTGTGACATC 
CGCCTGGACT GGGAGACCTA CACTCCAGAG GACAGGGAGG ACCGGCTGCT GGAGCTGCTG 
GTGTTCTATG GGCCGCCCTT CCCGCTACGT GACCACGCTG GGAATGACCT CCTGTACCCC 
GAGACCCCGG AGAGCAGCGG CTCCTTCTGG TCCCCAAGTC CCTCTGAGTC TGAGTCCAGC 
CGTGAAGCCA CCCCATCTGC CGAGGGCAGC TCCAGTGCTG GCGCCCCCCA GAAGATCATC 
TCAGTCATCC TGCGAGGGCA TGAGGGGGTG CCCTCTGACC ACCTGTTCTC CATCAGCCCC 
AAGCAGGTGG TGGTCCCTGC GCGGGGCAGC AGCACCATCC ACATCTCCTT CACACCCGTG 
GTGCTGGGCC CTGGCGTCCT GCACAAGGTG GAGTGTGTTG GCTATGCCCT GGGCTTCTTG 
AGCTTGGACG ATGAGGTGGA GAGGGAGCTT CCAGGCCGGA GGCGCCGCCT GCAGGACTTT 
GCCATGGGAC CCATGAGACT GGACCTGCAT GGCTACGTCA GGCCGGCGCA GCTGAGCGTG 
GACTGGGACT ACGGTGGCAG CATGGTGTTC CGGCAGCAGG CCAGCGACCT CATCCCTGAG 
CAGCCCTGCG CCGGGGTGCT GAGTGAGCTG GTGACCACCC GCCACCTGAA GCTGACCAAC 
ACCACGGAAA TCCCTCACTA CTTCTGGCTC CTGGTCTCCA AGCCCTTCTC CATTTCTCAA 
GACGGGGCGA GCCGCAGCCA CCGGGCCCTT GGCTGGAAGC AGGAGGGTGA GGAGATAGCA 
GCGAGGGGCA AGCAGCTGGT GCTCCACCCA CAGGAGAACA TGCTGGTGAA CGTGTCCTTC 
TCGCTCTCCC TGGAGCTGCT GTCCTATCAG AAGCTCCCGG CTGACCAGAT GCTGCCCGGA 
GTGGACATTC AGCAGAGCCC AAGTGGAGAG AAGCAGATGG TGTTCACACA GAACCTGCTG 
CTGGAATACA GCAACCAGAC CTCACAGGTG GTGCCCCTGC GGGCCGTGGT GGCCGTGCCC 
GAGCTGCAGC TCTCCACCAG CTGGGTGGAC TTCGGCCCCT GCTTCGTGAA CCAGGGCCGG 
GCCCGGGAGG TCTACCTCAT GAACCTGAGT GGCTGCCGAA GCTATTGGGC TGTGCTGACA 
GGCCAGCAGG AGCCAGACAG GGACCCCGTG GCCTTCAAGG TGTCCCCAAG CAGTGGGCTG 
CTGGAAGCAC GACCAGTCAA CGCACCCCCC ACCTCTGTCA CCCTCCAGGT TTCCTTCACC 
GCCAGGAGCT GTGAGCTGTA CGAGTCCACG CTGGTGGTGG AAGGCGTGCT CGGGGAGAAG 
CCGTGTACCC TGCGGCTCCG GGGCCGCGGC TCATATGACG AGAGACATGC TTCACCCCAG 
CACCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180127.3
CDS	1..5232
Translation	METGKRERQSAESGSYAFAGATWTPSPRLARIRSPSQSTWLSSLAYSESFHHGFGGRRRFVAQLSLPPLPEPQLLRLRPTSLRTQDISYLLTGVFRNLYTAEVIGEEVSSNLIKARGSEDARHEEFVDQLQQIRELYKQRLDEVEMLERHIIQARARALAEKERAMHQAKVQFLEPFIKMPPVKTIFRWCVDNELLRKHHLICPNDYYSDTVPFCSAPKGVSVPGYSKPTFSSEKRSIPKKELTKKPACRKMSSQSAEETDYTMDSLTWDSSFKAKLRSRAAIRKTGLPKNKNWMNHLRVPQRQLERLQLARMESRNRFLRNPRFFPPNTPLGGKSLLFPPKKPARVGAFQDGELEESCADTPVFLAKPSIGFFTDYEIGPVYEMVISLQNTTSTSRHLRVLPPSTPYFALGLGMFPGEGGMVAPGMTCQYIVQFFPDCLGDFDDFILVETQSAHTLLIPLQARRPPPVLTLSPVLDCGYCLIGGMKITRFICKNVGFSVGKFCIMPKKSWPPPSFRAVVTIGFVEQPPFGILPSVFELVPGQAVFVEVLFLPTSLGKAEQTFVIVCDNCQIKELVTTGVGQLVALDLIYISGEKSQPEPGELTDLTAQYFIRFEPENLQSTASKLLIIRNATHVELAFHWQIMKPNLQSLMPGETYSTDSIKYHPDRETAFCITPKMGVLSPHTDHEFILSFSPQEPRDFHSVLQMVLEEVPEPVSLDLENSKDPSYCMDDVIVLEIEVKGSVEPFQVLLEPYALIIPGENYIGINVKKNFKMWNNSKSTIRYTWGKISDCHIIEVEPCTGTIEPSEVEDFELNFTGGVPGPTSQNLLCEIKDSPTPVVLHIEAAFKGPALVIDVSALQFGLLRLGQKATKFIQIRNLSQLPATWAMKESQVSLQERQEGVSPFDIEPSSGQVQPLGECRVSITLEAQHCQRLQTVLELEVVDGTWSHLPVYAEVQEPYVYLQSSQVEVPNLYLGVPTKMAITLINGTLLPTRFHWGKLLGLQADVCTVKVSPRRGTLGPSEERQLSLELTAHTLEAVTDLALPCDVSGMREPLVLGISGKPRGLQVAIGISKEDSDDSTELWPGHPETLHLDFGSAVPLWTRVNRRLFLTNCSPIQTPFTLEFEYFGSPVNSLSQKPSPPDMPPALLKTARMRELLAKREQWEFMESMLSHGKGAAFFPHLSQGVLGAHQKLSIDLTACANMWGEYWDSLICTVGGLPPAVIPVRMAVVGCPISSQRTTYYTTDQTQREPIIRFGTRVSGGDPVTRILRLNNSSPCDIRLDWETYTPEDREDRLLELLVFYGPPFPLRDHAGNDLLYPETPESSGSFWSPSPSESESSREATPSAEGSSSAGAPQKIISVILRGHEGVPSDHLFSISPKQVVVPARGSSTIHISFTPVVLGPGVLHKVECVGYALGFLSLDDEVERELPGRRRRLQDFAMGPMRLDLHGYVRPAQLSVDWDYGGSMVFRQQASDLIPEQPCAGVLSELVTTRHLKLTNTTEIPHYFWLLVSKPFSISQDGASRSHRALGWKQEGEEIAARGKQLVLHPQENMLVNVSFSLSLELLSYQKLPADQMLPGVDIQQSPSGEKQMVFTQNLLLEYSNQTSQVVPLRAVVAVPELQLSTSWVDFGPCFVNQGRAREVYLMNLSGCRSYWAVLTGQQEPDRDPVAFKVSPSSGLLEARPVNAPPTSVTLQVSFTARSCELYESTLVVEGVLGEKPCTLRLRGRGSYDERHASPQHQL

Target ORF information:

RefSeq Version	NM_001193198.3
Organism	Bos taurus(cattle)
Definition	Bos taurus DLEC1 cilia and flagella associated protein (DLEC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193198.3

ORF Insert Sequence:

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCGCGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGGT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCATCTCTA AGGAGGACAG TGACGACAGC 
ACAGAGCTGT GGCCGGGCCA CCCCGAGACG CTCCACCTGG ACTTCGGCTC GGCAGTGCCA 
CTGTGGACCC GTGTGAACCG CCGGCTCTTC CTGACCAACT GCTCCCCGAT ACAGACCCCT 
TTCACCCTCG AGTTCGAGTA CTTTGGGAGC CCCGTGAACA GCCTGAGCCA GAAACCCAGC 
CCCCCCGACA TGCCTCCCGC CCTGCTGAAG ACGGCACGGA TGCGGGAGCT CTTGGCCAAG 
CGCGAGCAGT GGGAGTTTAT GGAAAGCATG CTGTCCCATG GGAAAGGAGC AGCCTTCTTC 
CCCCACCTCT CCCAGGGCGT GCTGGGCGCC CACCAGAAGC TCAGCATCGA CCTCACTGCC 
TGTGCCAACA TGTGGGGGGA GTACTGGGAT AGTCTCATCT GCACGGTGGG AGGCCTGCCT 
CCAGCAGTCA TCCCAGTGCG CATGGCGGTG GTGGGCTGCC CCATCAGCTC CCAGAGGACC 
ACCTACTACA CCACTGACCA GACCCAGAGG GAGCCCATCA TCAGGTTCGG CACCCGGGTC 
TCTGGAGGAG ACCCAGTCAC CCGGATCCTT CGCCTGAATA ATTCCAGCCC CTGTGACATC 
CGCCTGGACT GGGAGACCTA CACTCCAGAG GACAGGGAGG ACCGGCTGCT GGAGCTGCTG 
GTGTTCTATG GGCCGCCCTT CCCGCTACGT GACCACGCTG GGAATGACCT CCTGTACCCC 
GAGACCCCGG AGAGCAGCGG CTCCTTCTGG TCCCCAAGTC CCTCTGAGTC TGAGTCCAGC 
CGTGAAGCCA CCCCATCTGC CGAGGGCAGC TCCAGTGCTG GCGCCCCCCA GAAGATCATC 
TCAGTCATCC TGCGAGGGCA TGAGGGGGTG CCCTCTGACC ACCTGTTCTC CATCAGCCCC 
AAGCAGGTGG TGGTCCCTGC GCGGGGCAGC AGCACCATCC ACATCTCCTT CACACCCGTG 
GTGCTGGGCC CTGGCGTCCT GCACAAGGTG GAGTGTGTTG GCTATGCCCT GGGCTTCTTG 
AGCTTGGACG ATGAGGTGGA GAGGGAGCTT CCAGGCCGGA GGCGCCGCCT GCAGGACTTT 
GCCATGGGAC CCATGAGACT GGACCTGCAT GGCTACGTCA GGCCGGCGCA GCTGAGCGTG 
GACTGGGACT ACGGTGGCAG CATGGTGTTC CGGCAGCAGG CCAGCGACCT CATCCCTGAG 
CAGCCCTGCG CCGGGGTGCT GAGTGAGCTG GTGACCACCC GCCACCTGAA GCTGACCAAC 
ACCACGGAAA TCCCTCACTA CTTCTGGCTC CTGGTCTCCA AGCCCTTCTC CATTTCTCAA 
GACGGGGCGA GCCGCAGCCA CCGGGCCCTT GGCTGGAAGC AGGAGGGTGA GGAGATAGCA 
GCGAGGGGCA AGCAGCTGGT GCTCCACCCA CAGGAGAACA TGCTGGTGAA CGTGTCCTTC 
TCGCTCTCCC TGGAGCTGCT GTCCTATCAG AAGCTCCCGG CTGACCAGAT GCTGCCCGGA 
GTGGACATTC AGCAGAGCCC AAGTGGAGAG AAGCAGATGG TGTTCACACA GAACCTGCTG 
CTGGAATACA GCAACCAGAC CTCACAGGTG GTGCCCCTGC GGGCCGTGGT GGCCGTGCCC 
GAGCTGCAGC TCTCCACCAG CTGGGTGGAC TTCGGCCCCT GCTTCGTGAA CCAGGGCCGG 
GCCCGGGAGG TCTACCTCAT GAACCTGAGT GGCTGCCGAA GCTATTGGGC TGTGCTGACA 
GGCCAGCAGG AGCCAGACAG GGACCCCGTG GCCTTCAAGG TGTCCCCAAG CAGTGGGCTG 
CTGGAAGCAC GACCAGTCAA CGCACCCCCC ACCTCTGTCA CCCTCCAGGT TTCCTTCACC 
GCCAGGAGCT GTGAGCTGTA CGAGTCCACG CTGGTGGTGG AAGGCGTGCT CGGGGAGAAG 
CCGTGTACCC TGCGGCTCCG GGGCCGCGGC TCATATGACG AGAGACATGC TTCACCCCAG 
CACCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLEC1 ( XM_005222552.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222609.2 Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X1, mRNA.

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CloneID	OBa229172
Clone ID Related Accession (Same CDS sequence)	XM_005222552.4
Accession Version	XM_005222552.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	deleted in lung and esophageal cancer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037349.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005222552.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCGCGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGGT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCATCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GGTCCCTGCG CGGGGCAGCA GCACCATCCA CATCTCCTTC 
ACACCCGTGG TGCTGGGCCC TGGCGTCCTG CACAAGGTGG AGTGTGTTGG CTATGCCCTG 
GGCTTCTTGA GCTTGGACGA TGAGGTGGAG AGGGAGCTTC CAGGCCGGAG GCGCCGCCTG 
CAGGACTTTG CCATGGGACC CATGAGACTG GACCTGCATG GCTACGTCAG GCCGGCGCAG 
CTGAGCGTGG ACTGGGACTA CGGTGGCAGC ATGGTGTTCC GGCAGCAGGC CAGCGACCTC 
ATCCCTGAGC AGCCCTGCGC CGGGGTGCTG AGTGAGCTGG TGACCACCCG CCACCTGAAG 
CTGACCAACA CCACGGAAAT CCCTCACTAC TTCTGGCTCC TGGTCTCCAA GCCCTTCTCC 
ATTTCTCAAG ACGGGGCGAG CCGCAGCCAC CGGGCCCTTG GCTGGAAGCA GGAGGGTGAG 
GAGATAGCAG CGAGGGGCAA GCAGCTGGTG CTCCACCCAC AGGAGAACAT GCTGGTGAAC 
GTGTCCTTCT CGCTCTCCCT GGAGCTGCTG TCCTATCAGA AGCTCCCGGC TGACCAGATG 
CTGCCCGGAG TGGACATTCA GCAGAGCCCA AGTGGAGAGA AGCAGATGGT GTTCACACAG 
AACCTGCTGC TGGAATACAG CAACCAGACC TCACAGGTGG TGCCCCTGCG GGCCGTGGTG 
GCCGTGCCCG AGCTGCAGCT CTCCACCAGC TGGGTGGACT TCGGCCCCTG CTTCGTGAAC 
CAGGGCCGGG CCCGGGAGGT CTACCTCATG AACCTGAGTG GCTGCCGAAG CTATTGGGCT 
GTGCTGACAG GCCAGCAGGA GCCAGACAGG GACCCCGTGG CCTTCAAGGT GTCCCCAAGC 
AGTGGGCTGC TGGAAGCACG ACCAGTCAAC GCACCCCCCA CCTCTGTCAC CCTCCAGGTT 
TCCTTCACCG CCAGGAGCTG TGAGCTGTAC GAGTCCACGC TGGTGGTGGA AGGCGTGCTC 
GGGGAGAAGC CGTGTACCCT GCGGCTCCGG GGCCGCGGCT CATATGACGA GAGACATGCT 
TCACCCCAGC ACCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222609.2
CDS	1..5241
Translation	METGKRERQSAESGSYAFAGATWTPSPRLARIRSPSQSTWLSSLAYSESFHHGFGGRRRFVAQLSLPPLPEPQLLRLRPTSLRTQDISYLLTGVFRNLYTAEVIGEEVSSNLIKARGSEDARHEEFVDQLQQIRELYKQRLDEVEMLERHIIQARARALAEKERAMHQAKVQFLEPFIKMPPVKTIFRWCVDNELLRKHHLICPNDYYSDTVPFCSAPKGVSVPGYSKPTFSSEKRSIPKKELTKKPACRKMSSQSAEETDYTMDSLTWDSSFKAKLRSRAAIRKTGLPKNKNWMNHLRVPQRQLERLQLARMESRNRFLRNPRFFPPNTPLGGKSLLFPPKKPARVGAFQDGELEESCADTPVFLAKPSIGFFTDYEIGPVYEMVISLQNTTSTSRHLRVLPPSTPYFALGLGMFPGEGGMVAPGMTCQYIVQFFPDCLGDFDDFILVETQSAHTLLIPLQARRPPPVLTLSPVLDCGYCLIGGMKITRFICKNVGFSVGKFCIMPKKSWPPPSFRAVVTIGFVEQPPFGILPSVFELVPGQAVFVEVLFLPTSLGKAEQTFVIVCDNCQIKELVTTGVGQLVALDLIYISGEKSQPEPGELTDLTAQYFIRFEPENLQSTASKLLIIRNATHVELAFHWQIMKPNLQSLMPGETYSTDSIKYHPDRETAFCITPKMGVLSPHTDHEFILSFSPQEPRDFHSVLQMVLEEVPEPVSLDLENSKDPSYCMDDVIVLEIEVKGSVEPFQVLLEPYALIIPGENYIGINVKKNFKMWNNSKSTIRYTWGKISDCHIIEVEPCTGTIEPSEVEDFELNFTGGVPGPTSQNLLCEIKDSPTPVVLHIEAAFKGPALVIDVSALQFGLLRLGQKATKFIQIRNLSQLPATWAMKESQVSLQERQEGVSPFDIEPSSGQVQPLGECRVSITLEAQHCQRLQTVLELEVVDGTWSHLPVYAEVQEPYVYLQSSQVEVPNLYLGVPTKMAITLINGTLLPTRFHWGKLLGLQADVCTVKVSPRRGTLGPSEERQLSLELTAHTLEAVTDLALPCDVSGMREPLVLGISGKPRGLQVAIGISKEDSDDSAPSTELWPGHPETLHLDFGSAVPLWTRVNRRLFLTNCSPIQTPFTLEFEYFGSPVNSLSQKPSPPDMPPALLKTARMRELLAKREQWEFMESMLSHGKGAAFFPHLSQGVLGAHQKLSIDLTACANMWGEYWDSLICTVGGLPPAVIPVRMAVVGCPISSQRTTYYTTDQTQREPIIRFGTRVSGGDPVTRILRLNNSSPCDIRLDWETYTPEDREDRLLELLVFYGPPFPLRDHAGNDLLYPETPESSGSFWSPSPSESESSREATPSAEGSSSAGAPQKIISVILRGHEGVPSDHLFSISPKQVVVPARGSSTIHISFTPVVLGPGVLHKVECVGYALGFLSLDDEVERELPGRRRRLQDFAMGPMRLDLHGYVRPAQLSVDWDYGGSMVFRQQASDLIPEQPCAGVLSELVTTRHLKLTNTTEIPHYFWLLVSKPFSISQDGASRSHRALGWKQEGEEIAARGKQLVLHPQENMLVNVSFSLSLELLSYQKLPADQMLPGVDIQQSPSGEKQMVFTQNLLLEYSNQTSQVVPLRAVVAVPELQLSTSWVDFGPCFVNQGRAREVYLMNLSGCRSYWAVLTGQQEPDRDPVAFKVSPSSGLLEARPVNAPPTSVTLQVSFTARSCELYESTLVVEGVLGEKPCTLRLRGRGSYDERHASPQHQL

Target ORF information:

RefSeq Version	XM_005222552.4
Organism	Bos taurus(cattle)
Definition	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222552.4

ORF Insert Sequence:

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCGCGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGGT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCATCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GGTCCCTGCG CGGGGCAGCA GCACCATCCA CATCTCCTTC 
ACACCCGTGG TGCTGGGCCC TGGCGTCCTG CACAAGGTGG AGTGTGTTGG CTATGCCCTG 
GGCTTCTTGA GCTTGGACGA TGAGGTGGAG AGGGAGCTTC CAGGCCGGAG GCGCCGCCTG 
CAGGACTTTG CCATGGGACC CATGAGACTG GACCTGCATG GCTACGTCAG GCCGGCGCAG 
CTGAGCGTGG ACTGGGACTA CGGTGGCAGC ATGGTGTTCC GGCAGCAGGC CAGCGACCTC 
ATCCCTGAGC AGCCCTGCGC CGGGGTGCTG AGTGAGCTGG TGACCACCCG CCACCTGAAG 
CTGACCAACA CCACGGAAAT CCCTCACTAC TTCTGGCTCC TGGTCTCCAA GCCCTTCTCC 
ATTTCTCAAG ACGGGGCGAG CCGCAGCCAC CGGGCCCTTG GCTGGAAGCA GGAGGGTGAG 
GAGATAGCAG CGAGGGGCAA GCAGCTGGTG CTCCACCCAC AGGAGAACAT GCTGGTGAAC 
GTGTCCTTCT CGCTCTCCCT GGAGCTGCTG TCCTATCAGA AGCTCCCGGC TGACCAGATG 
CTGCCCGGAG TGGACATTCA GCAGAGCCCA AGTGGAGAGA AGCAGATGGT GTTCACACAG 
AACCTGCTGC TGGAATACAG CAACCAGACC TCACAGGTGG TGCCCCTGCG GGCCGTGGTG 
GCCGTGCCCG AGCTGCAGCT CTCCACCAGC TGGGTGGACT TCGGCCCCTG CTTCGTGAAC 
CAGGGCCGGG CCCGGGAGGT CTACCTCATG AACCTGAGTG GCTGCCGAAG CTATTGGGCT 
GTGCTGACAG GCCAGCAGGA GCCAGACAGG GACCCCGTGG CCTTCAAGGT GTCCCCAAGC 
AGTGGGCTGC TGGAAGCACG ACCAGTCAAC GCACCCCCCA CCTCTGTCAC CCTCCAGGTT 
TCCTTCACCG CCAGGAGCTG TGAGCTGTAC GAGTCCACGC TGGTGGTGGA AGGCGTGCTC 
GGGGAGAAGC CGTGTACCCT GCGGCTCCGG GGCCGCGGCT CATATGACGA GAGACATGCT 
TCACCCCAGC ACCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLEC1 ( NM_001193198.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180127.1 Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), mRNA.

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CloneID	OBa01789
Clone ID Related Accession (Same CDS sequence)	NM_001193198.1
Accession Version	NM_001193198.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5232bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2011-11-15
Organism	Bos taurus(Cattle)
Product	deleted in lung and esophageal cancer protein 1
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03064905.1 and AAFC03034510.1. On Jul 17, 2010 this sequence version replaced XM_871071.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCACGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGGT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCAG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCGTCTCTA AGGAGGACAG TGACGACAGC 
ACAGAGCTGT GGCCGGGCCA CCCCGAGACG CTCCACCTGG ACTTCGGCTC GGCAGTGCCA 
CTGTGGACCC GTGTGAACCG CCGGCTCTTC CTGACCAACT GCTCCCCGAT ACAGACCCCT 
TTCACCCTCG AGTTCGAGTA CTTTGGGAGC CCCGTGAACA GCCTGAGCCA GAAACCCAGC 
CCCCCCGACA TGCCTCCCGC CCTGCTGAAG ACGGCACGGA TGCGGGAGCT CTTGGCCAAG 
CGCGAGCAGT GGGAGTTTAT GGAAAGCATG CTGTCCCATG GGAAAGGAGC AGCCTTCTTC 
CCCCACCTCT CCCAGGGCGT GCTGGGCGCC CACCAGAAGC TCAGCATCGA CCTCACTGCC 
TGTGCCAACA TGTGGGGGGA GTACTGGGAT AGTCTCATCT GCACGGTGGG AGGCCTGCCT 
CCAGCAGTCA TCCCAGTGCG CATGGCGGTG GTGGGCTGCC CCATCAGCTC CCAGAGGACC 
ACCTACTACA CCACTGACCA GACCCAGAGG GAGCCCATCA TCAGGTTCGG CACCCGGGTC 
TCTGGAGGAG ACCCAGTCAC CCGGATCCTT CGCCTGAATA ATTCCAGCCC CTGTGACATC 
CGCCTGGACT GGGAGACCTA CACTCCAGAG GACAGGGAGG ACCGGCTGCT GGAGCTGCTG 
GTGTTCTATG GGCCGCCCTT CCCGCTACGT GACCACGCTG GGAATGACCT CCTGTACCCC 
GAGACCCCGG AGAGCAGCGG CTCCTTCTGG TCCCCAAGTC CCTCTGAGTC TGAGTCCAGC 
CGTGAAGCCA CCCCATCTGC CGAGGGCAGC TCCAGTGCTG GCGCCCCCCA GAAGATCATC 
TCAGTCATCC TGCGAGGGCA TGAGGGGGTG CCCTCTGACC ACCTGTTCTC CATCAGCCCC 
AAGCAGGTGG TGGTCCCTGC GCGGGGCAGC AGCACCATCC ACATCTCCTT CACACCCGTG 
GTGCTGGGCC CTGGCGTCCT GCACAAGGTG GAGTGTGTTG GCTATGCCCT GGGCTTCTTG 
AGCTTGGACG ATGAGGTGGA GAGGGAGCTT CCAGGCCGGA GGCGCCGCCT GCAGGACTTT 
GCCATGGGAC CCATGAGACT GGACCTGCAT GGCTACGTCA GGCCGGCGCA GCTGAGCGTG 
GACTGGGACT ACGGTGGCAG CATGGTGTTC CGGCAGCAGG CCAGCGACCT CATCCCTGAG 
CAGCCCTGCG CCGGGGTGCT GAGTGAGCTG GTGACCACCC GCCACCTGAA GCTGACCAAC 
ACCACGGAAA TCCCTCACTA CTTCTGGCTC CTGGTCTCCA AGCCCTTCTC CATTTCTCAA 
GACGGGGCGA GCCGCAGCCA CCGGGCCCTT GGCTGGAAGC AGGAGGGTGA GGAGATAGCA 
GCGAGGGGCA AGCAGCTGGT GCTCCACCCA CAGGAGAACA TGCTGGTGAA CGTGTCCTTC 
TCGCTCTCCC TGGAGCTGCT GTCCTATCAG AAGCTCCCGG CTGACCAGAT GCTGCCCGGA 
GTGGACATTC AGCAGAGCCC AAGTGGAGAG AAGCAGATGG TGTTCACACA GAACCTGCTG 
CTGGAATACA GCAACCAGAC CTCACAGGTG GTGCCCCTGC GGGCCGTGGT GGCCGTGCCC 
GAGCTGCAGC TCTCCACCAG CTGGGTGGAC TTCGGCCCCT GCTTCGTGAA CCAGGGCCGG 
GCCCGGGAGG TCTACCTCAT GAACCTGAGT GGCTGCCGAA GCTATTGGGC TGTGCTGACA 
GGCCAGCAGG AGCCAGACAG GGACCCCGTG GCCTTCAAGG TGTCCCCAAG CAGTGGGCTG 
CTGGAAGCAC GACCAGTCAA CGCACCCCCC ACCTCTGTCA CCCTCCAGGT TTCCTTCACC 
GCCAGGAGCT GTGAGCTGTA CGAGTCCACG CTGGTGGTGG AAGGCGTGCT CGGGGAGAAG 
CCGTGTACCC TGCGGCTCCG GGGCCGCGGC TCATATGACG AGAGACATGC TTCACCCCAG 
CACCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180127.1
CDS	1..5232
Translation	METGKRERQSAESGSYAFAGATWTPSPRLARIRSPSQSTWLSSLAYSESFHHGFGGRRRFVAQLSLPPLPEPQLLRLRPTSLRTQDISYLLTGVFRNLYTAEVIGEEVSSNLIKARGSEDARHEEFVDQLQQIRELYKQRLDEVEMLERHIIQARARALAEKERAMHQAKVQFLEPFIKMPPVKTIFRWCVDNELLRKHHLICPNDYYSDTVPFCSAPKGVSVPGYSKPTFSSEKRSIPKKELTKKPACRKMSSQSAEETDYTMDSLTWDSSFKAKLRSRAAIRKTGLPKNKNWMNHLRVPQRQLERLQLARMESRNRFLRNPRFFPPNTPLGGKSLLFPPKKPARVGAFQDGELEESCADTPVFLAKPSIGFFTDYEIGPVYEMVISLQNTTSTSRHLRVLPPSTPYFALGLGMFPGEGGMVAPGMTCQYIVQFFPDCLGDFDDFILVETQSAHTLLIPLQARRPPPVLTLSPVLDCGYCLIGGMKITRFICKNVGFSVGKFCIMPKKSWPPPSFRAVVTIGFVEQPPFGILPSVFELVPGQAVFVEVLFLPTSLGKAEQTFVIVCDNCQIKELVTTGVGQLVALDLIYISGEKSQPEPGELTDLTAQYFIRFEPENLQSTASKLLIIRNATHVELAFHWQIMKPNLQSLMPGETYSTDSIKYHPDRETAFCITPKMGVLSPHTDHEFILSFSPQEPRDFHSVLQMVLEEVPEPVSLDLENSKDPSYCMDDVIVLEIEVKGSVEPFQVLLEPYALIIPGENYIGINVKKNFKMWNNSKSTIRYTWGKISDCHIIEVEPCTGTIEPSEVEDFELNFTGGVPGPTSQNLLCEIKDSPTPVVLHIEAAFKGPALVIDVSALQFGLLRLGQKATKFIQIRNLSQLPATWAMKESQVSLQERQEGVSPFDIEPSSGQVQPLGECRVSITLEAQHCQRLQTVLELEVVDGTWSHLPVYAEVQEPYVYLQSSQVEVPNLYLGVPTKMAITLINGTLLPTRFHWGKLLGLQADVCTVKVSPRRGTLGPSEERQLSLELTAHTLEAVTDLALPCDVSGMREPLVLGISGKPRGLQVAIGVSKEDSDDSTELWPGHPETLHLDFGSAVPLWTRVNRRLFLTNCSPIQTPFTLEFEYFGSPVNSLSQKPSPPDMPPALLKTARMRELLAKREQWEFMESMLSHGKGAAFFPHLSQGVLGAHQKLSIDLTACANMWGEYWDSLICTVGGLPPAVIPVRMAVVGCPISSQRTTYYTTDQTQREPIIRFGTRVSGGDPVTRILRLNNSSPCDIRLDWETYTPEDREDRLLELLVFYGPPFPLRDHAGNDLLYPETPESSGSFWSPSPSESESSREATPSAEGSSSAGAPQKIISVILRGHEGVPSDHLFSISPKQVVVPARGSSTIHISFTPVVLGPGVLHKVECVGYALGFLSLDDEVERELPGRRRRLQDFAMGPMRLDLHGYVRPAQLSVDWDYGGSMVFRQQASDLIPEQPCAGVLSELVTTRHLKLTNTTEIPHYFWLLVSKPFSISQDGASRSHRALGWKQEGEEIAARGKQLVLHPQENMLVNVSFSLSLELLSYQKLPADQMLPGVDIQQSPSGEKQMVFTQNLLLEYSNQTSQVVPLRAVVAVPELQLSTSWVDFGPCFVNQGRAREVYLMNLSGCRSYWAVLTGQQEPDRDPVAFKVSPSSGLLEARPVNAPPTSVTLQVSFTARSCELYESTLVVEGVLGEKPCTLRLRGRGSYDERHASPQHQL

Target ORF information:

RefSeq Version	NM_001193198.1
Organism	Bos taurus(Cattle)
Definition	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193198.1

ORF Insert Sequence:

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCACGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGGT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCAG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCGTCTCTA AGGAGGACAG TGACGACAGC 
ACAGAGCTGT GGCCGGGCCA CCCCGAGACG CTCCACCTGG ACTTCGGCTC GGCAGTGCCA 
CTGTGGACCC GTGTGAACCG CCGGCTCTTC CTGACCAACT GCTCCCCGAT ACAGACCCCT 
TTCACCCTCG AGTTCGAGTA CTTTGGGAGC CCCGTGAACA GCCTGAGCCA GAAACCCAGC 
CCCCCCGACA TGCCTCCCGC CCTGCTGAAG ACGGCACGGA TGCGGGAGCT CTTGGCCAAG 
CGCGAGCAGT GGGAGTTTAT GGAAAGCATG CTGTCCCATG GGAAAGGAGC AGCCTTCTTC 
CCCCACCTCT CCCAGGGCGT GCTGGGCGCC CACCAGAAGC TCAGCATCGA CCTCACTGCC 
TGTGCCAACA TGTGGGGGGA GTACTGGGAT AGTCTCATCT GCACGGTGGG AGGCCTGCCT 
CCAGCAGTCA TCCCAGTGCG CATGGCGGTG GTGGGCTGCC CCATCAGCTC CCAGAGGACC 
ACCTACTACA CCACTGACCA GACCCAGAGG GAGCCCATCA TCAGGTTCGG CACCCGGGTC 
TCTGGAGGAG ACCCAGTCAC CCGGATCCTT CGCCTGAATA ATTCCAGCCC CTGTGACATC 
CGCCTGGACT GGGAGACCTA CACTCCAGAG GACAGGGAGG ACCGGCTGCT GGAGCTGCTG 
GTGTTCTATG GGCCGCCCTT CCCGCTACGT GACCACGCTG GGAATGACCT CCTGTACCCC 
GAGACCCCGG AGAGCAGCGG CTCCTTCTGG TCCCCAAGTC CCTCTGAGTC TGAGTCCAGC 
CGTGAAGCCA CCCCATCTGC CGAGGGCAGC TCCAGTGCTG GCGCCCCCCA GAAGATCATC 
TCAGTCATCC TGCGAGGGCA TGAGGGGGTG CCCTCTGACC ACCTGTTCTC CATCAGCCCC 
AAGCAGGTGG TGGTCCCTGC GCGGGGCAGC AGCACCATCC ACATCTCCTT CACACCCGTG 
GTGCTGGGCC CTGGCGTCCT GCACAAGGTG GAGTGTGTTG GCTATGCCCT GGGCTTCTTG 
AGCTTGGACG ATGAGGTGGA GAGGGAGCTT CCAGGCCGGA GGCGCCGCCT GCAGGACTTT 
GCCATGGGAC CCATGAGACT GGACCTGCAT GGCTACGTCA GGCCGGCGCA GCTGAGCGTG 
GACTGGGACT ACGGTGGCAG CATGGTGTTC CGGCAGCAGG CCAGCGACCT CATCCCTGAG 
CAGCCCTGCG CCGGGGTGCT GAGTGAGCTG GTGACCACCC GCCACCTGAA GCTGACCAAC 
ACCACGGAAA TCCCTCACTA CTTCTGGCTC CTGGTCTCCA AGCCCTTCTC CATTTCTCAA 
GACGGGGCGA GCCGCAGCCA CCGGGCCCTT GGCTGGAAGC AGGAGGGTGA GGAGATAGCA 
GCGAGGGGCA AGCAGCTGGT GCTCCACCCA CAGGAGAACA TGCTGGTGAA CGTGTCCTTC 
TCGCTCTCCC TGGAGCTGCT GTCCTATCAG AAGCTCCCGG CTGACCAGAT GCTGCCCGGA 
GTGGACATTC AGCAGAGCCC AAGTGGAGAG AAGCAGATGG TGTTCACACA GAACCTGCTG 
CTGGAATACA GCAACCAGAC CTCACAGGTG GTGCCCCTGC GGGCCGTGGT GGCCGTGCCC 
GAGCTGCAGC TCTCCACCAG CTGGGTGGAC TTCGGCCCCT GCTTCGTGAA CCAGGGCCGG 
GCCCGGGAGG TCTACCTCAT GAACCTGAGT GGCTGCCGAA GCTATTGGGC TGTGCTGACA 
GGCCAGCAGG AGCCAGACAG GGACCCCGTG GCCTTCAAGG TGTCCCCAAG CAGTGGGCTG 
CTGGAAGCAC GACCAGTCAA CGCACCCCCC ACCTCTGTCA CCCTCCAGGT TTCCTTCACC 
GCCAGGAGCT GTGAGCTGTA CGAGTCCACG CTGGTGGTGG AAGGCGTGCT CGGGGAGAAG 
CCGTGTACCC TGCGGCTCCG GGGCCGCGGC TCATATGACG AGAGACATGC TTCACCCCAG 
CACCAGCTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLEC1 ( XM_005222552.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222609.1 Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X1, mRNA.

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CloneID	OBa31377
Clone ID Related Accession (Same CDS sequence)	XM_005222552.3
Accession Version	XM_005222552.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	deleted in lung and esophageal cancer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104540.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222552.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCACGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGAT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCGTCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GGTCCCTGCG CGGGGCAGCA GCACCATCCA CATCTCCTTC 
ACACCCGTGG TGCTGGGCCC TGGCGTCCTG CACAAGGTGG AGTGTGTTGG CTATGCCCTG 
GGCTTCTTGA GCTTGGACGA TGAGGTGGAG AGGGAGCTTC CAGGCCGGAG GCGCCGCCTG 
CAGGACTTTG CCATGGGACC CATGAGACTG GACCTGCATG GCTACGTCAG GCCGGCGCAG 
CTGAGCGTGG ACTGGGACTA CGGTGGCAGC ATGGTGTTCC GGCAGCAGGC CAGCGACCTC 
ATCCCTGAGC AGCCCTGCGC CGGGGTGCTG AGTGAGCTGG TGACCACCCG CCACCTGAAG 
CTGACCAACA CCACGGAAAT CCCTCACTAC TTCTGGCTCC TGGTCTCCAA GCCCTTCTCC 
ATTTCTCAAG ACGGGGCGAG CCGCAGCCAC CGGGCCCTTG GCTGGAAGCA GGAGGGTGAG 
GAGATAGCAG CGAGGGGCAA GCAGCTGGTG CTCCACCCAC AGGAGAACAT GCTGGTGAAC 
GTGTCCTTCT CGCTCTCCCT GGAGCTGCTG TCCTATCAGA AGCTCCCGGC TGACCAGATG 
CTGCCCGGAG TGGACATTCA GCAGAGCCCA AGTGGAGAGA AGCAGATGGT GTTCACACAG 
AACCTGCTGC TGGAATACAG CAACCAGACC TCACAGGTGG TGCCCCTGCG GGCCGTGGTG 
GCCGTGCCCG AGCTGCAGCT CTCCACCAGC TGGGTGGACT TCGGCCCCTG CTTCGTGAAC 
CAGGGCCGGG CCCGGGAGGT CTACCTCATG AACCTGAGTG GCTGCCGAAG CTATTGGGCT 
GTGCTGACAG GCCAGCAGGA GCCAGACAGG GACCCCGTGG CCTTCAAGGT GTCCCCAAGC 
AGTGGGCTGC TGGAAGCACG ACCAGTCAAC GCACCCCCCA CCTCTGTCAC CCTCCAGGTT 
TCCTTCACCG CCAGGAGCTG TGAGCTGTAC GAGTCCACGC TGGTGGTGGA AGGCGTGCTC 
GGGGAGAAGC CGTGTACCCT GCGGCTCCGG GGCCGCGGCT CATATGACGA GAGACATGCT 
TCACCCCAGC ACCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222609.1
CDS	182..5422
Translation	METGKRERQSAESGSYAFAGATWTPSPRLARIRSPSQSTWLSSLAYSESFHHGFGGRRRFVAQLSLPPLPEPQLLRLRPTSLRTQDISYLLTGVFRNLYTAEVIGEEVSSNLIKARGSEDARHEEFVDQLQQIRELYKQRLDEVEMLERHIIQARARALAEKERAMHQAKVQFLEPFIKMPPVKTIFRWCVDNELLRKHHLICPNDYYSDTVPFCSAPKGVSVPGYSKPTFSSEKRSIPKKELTKKPACRKMSSQSAEETDYTMDSLTWDSSFKAKLRSRAAIRKTGLPKNKNWMNHLRVPQRQLERLQLARMESRNRFLRNPRFFPPNTPLGGKSLLFPPKKPARIGAFQDGELEESCADTPVFLAKPSIGFFTDYEIGPVYEMVISLQNTTSTSRHLRVLPPSTPYFALGLGMFPGEGGMVAPGMTCQYIVQFFPDCLGDFDDFILVETQSAHTLLIPLQARRPPPVLTLSPVLDCGYCLIGGMKITRFICKNVGFSVGKFCIMPKKSWPPPSFRAVVTIGFVEQPPFGILPSVFELVPGQAVFVEVLFLPTSLGKAEQTFVIVCDNCQIKELVTTGVGQLVALDLIYISGEKSQPEPGELTDLTAQYFIRFEPENLQSTASKLLIIRNATHVELAFHWQIMKPNLQSLMPGETYSTDSIKYHPDRETAFCITPKMGVLSPHTDHEFILSFSPQEPRDFHSVLQMVLEEVPEPVSLDLENSKDPSYCMDDVIVLEIEVKGSVEPFQVLLEPYALIIPGENYIGINVKKNFKMWNNSKSTIRYTWGKISDCHIIEVEPCTGTIEPSEVEDFELNFTGGVPGPTSQNLLCEIKDSPTPVVLHIEAAFKGPALVIDVSALQFGLLRLGQKATKFIQIRNLSQLPATWAMKESQVSLQERQEGVSPFDIEPSSGQVQPLGECRVSITLEAQHCQRLQTVLELEVVDGTWSHLPVYAEVQEPYVYLQSSQVEVPNLYLGVPTKMAITLINGTLLPTRFHWGKLLGLQADVCTVKVSPRRGTLGPSEERQLSLELTAHTLEAVTDLALPCDVSGMREPLVLGISGKPRGLQVAIGVSKEDSDDSAPSTELWPGHPETLHLDFGSAVPLWTRVNRRLFLTNCSPIQTPFTLEFEYFGSPVNSLSQKPSPPDMPPALLKTARMRELLAKREQWEFMESMLSHGKGAAFFPHLSQGVLGAHQKLSIDLTACANMWGEYWDSLICTVGGLPPAVIPVRMAVVGCPISSQRTTYYTTDQTQREPIIRFGTRVSGGDPVTRILRLNNSSPCDIRLDWETYTPEDREDRLLELLVFYGPPFPLRDHAGNDLLYPETPESSGSFWSPSPSESESSREATPSAEGSSSAGAPQKIISVILRGHEGVPSDHLFSISPKQVVVPARGSSTIHISFTPVVLGPGVLHKVECVGYALGFLSLDDEVERELPGRRRRLQDFAMGPMRLDLHGYVRPAQLSVDWDYGGSMVFRQQASDLIPEQPCAGVLSELVTTRHLKLTNTTEIPHYFWLLVSKPFSISQDGASRSHRALGWKQEGEEIAARGKQLVLHPQENMLVNVSFSLSLELLSYQKLPADQMLPGVDIQQSPSGEKQMVFTQNLLLEYSNQTSQVVPLRAVVAVPELQLSTSWVDFGPCFVNQGRAREVYLMNLSGCRSYWAVLTGQQEPDRDPVAFKVSPSSGLLEARPVNAPPTSVTLQVSFTARSCELYESTLVVEGVLGEKPCTLRLRGRGSYDERHASPQHQL

Target ORF information:

RefSeq Version	XM_005222552.3
Organism	Bos taurus(cattle)
Definition	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222552.3

ORF Insert Sequence:

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCACGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGAT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCGTCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GGTCCCTGCG CGGGGCAGCA GCACCATCCA CATCTCCTTC 
ACACCCGTGG TGCTGGGCCC TGGCGTCCTG CACAAGGTGG AGTGTGTTGG CTATGCCCTG 
GGCTTCTTGA GCTTGGACGA TGAGGTGGAG AGGGAGCTTC CAGGCCGGAG GCGCCGCCTG 
CAGGACTTTG CCATGGGACC CATGAGACTG GACCTGCATG GCTACGTCAG GCCGGCGCAG 
CTGAGCGTGG ACTGGGACTA CGGTGGCAGC ATGGTGTTCC GGCAGCAGGC CAGCGACCTC 
ATCCCTGAGC AGCCCTGCGC CGGGGTGCTG AGTGAGCTGG TGACCACCCG CCACCTGAAG 
CTGACCAACA CCACGGAAAT CCCTCACTAC TTCTGGCTCC TGGTCTCCAA GCCCTTCTCC 
ATTTCTCAAG ACGGGGCGAG CCGCAGCCAC CGGGCCCTTG GCTGGAAGCA GGAGGGTGAG 
GAGATAGCAG CGAGGGGCAA GCAGCTGGTG CTCCACCCAC AGGAGAACAT GCTGGTGAAC 
GTGTCCTTCT CGCTCTCCCT GGAGCTGCTG TCCTATCAGA AGCTCCCGGC TGACCAGATG 
CTGCCCGGAG TGGACATTCA GCAGAGCCCA AGTGGAGAGA AGCAGATGGT GTTCACACAG 
AACCTGCTGC TGGAATACAG CAACCAGACC TCACAGGTGG TGCCCCTGCG GGCCGTGGTG 
GCCGTGCCCG AGCTGCAGCT CTCCACCAGC TGGGTGGACT TCGGCCCCTG CTTCGTGAAC 
CAGGGCCGGG CCCGGGAGGT CTACCTCATG AACCTGAGTG GCTGCCGAAG CTATTGGGCT 
GTGCTGACAG GCCAGCAGGA GCCAGACAGG GACCCCGTGG CCTTCAAGGT GTCCCCAAGC 
AGTGGGCTGC TGGAAGCACG ACCAGTCAAC GCACCCCCCA CCTCTGTCAC CCTCCAGGTT 
TCCTTCACCG CCAGGAGCTG TGAGCTGTAC GAGTCCACGC TGGTGGTGGA AGGCGTGCTC 
GGGGAGAAGC CGTGTACCCT GCGGCTCCGG GGCCGCGGCT CATATGACGA GAGACATGCT 
TCACCCCAGC ACCAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DLEC1 ( XM_005222554.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005222611.1 Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X2, mRNA.

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CloneID	OBa31379
Clone ID Related Accession (Same CDS sequence)	XM_005222554.3
Accession Version	XM_005222554.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	deleted in lung and esophageal cancer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104540.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005222554.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCACGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGAT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCGTCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GGTCCCTGCG CGGGGCAGCA GCACCATCCA CATCTCCTTC 
ACACCCGTGG TGCTGGGCCC TGGCGTCCTG CACAAGGTGG AGTGTGTTGG CTATGCCCTG 
GGCTTCTTGA GCTTGGACGA TGAGGACTCC AGTGATGGGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005222611.1
CDS	181..4485
Translation	METGKRERQSAESGSYAFAGATWTPSPRLARIRSPSQSTWLSSLAYSESFHHGFGGRRRFVAQLSLPPLPEPQLLRLRPTSLRTQDISYLLTGVFRNLYTAEVIGEEVSSNLIKARGSEDARHEEFVDQLQQIRELYKQRLDEVEMLERHIIQARARALAEKERAMHQAKVQFLEPFIKMPPVKTIFRWCVDNELLRKHHLICPNDYYSDTVPFCSAPKGVSVPGYSKPTFSSEKRSIPKKELTKKPACRKMSSQSAEETDYTMDSLTWDSSFKAKLRSRAAIRKTGLPKNKNWMNHLRVPQRQLERLQLARMESRNRFLRNPRFFPPNTPLGGKSLLFPPKKPARIGAFQDGELEESCADTPVFLAKPSIGFFTDYEIGPVYEMVISLQNTTSTSRHLRVLPPSTPYFALGLGMFPGEGGMVAPGMTCQYIVQFFPDCLGDFDDFILVETQSAHTLLIPLQARRPPPVLTLSPVLDCGYCLIGGMKITRFICKNVGFSVGKFCIMPKKSWPPPSFRAVVTIGFVEQPPFGILPSVFELVPGQAVFVEVLFLPTSLGKAEQTFVIVCDNCQIKELVTTGVGQLVALDLIYISGEKSQPEPGELTDLTAQYFIRFEPENLQSTASKLLIIRNATHVELAFHWQIMKPNLQSLMPGETYSTDSIKYHPDRETAFCITPKMGVLSPHTDHEFILSFSPQEPRDFHSVLQMVLEEVPEPVSLDLENSKDPSYCMDDVIVLEIEVKGSVEPFQVLLEPYALIIPGENYIGINVKKNFKMWNNSKSTIRYTWGKISDCHIIEVEPCTGTIEPSEVEDFELNFTGGVPGPTSQNLLCEIKDSPTPVVLHIEAAFKGPALVIDVSALQFGLLRLGQKATKFIQIRNLSQLPATWAMKESQVSLQERQEGVSPFDIEPSSGQVQPLGECRVSITLEAQHCQRLQTVLELEVVDGTWSHLPVYAEVQEPYVYLQSSQVEVPNLYLGVPTKMAITLINGTLLPTRFHWGKLLGLQADVCTVKVSPRRGTLGPSEERQLSLELTAHTLEAVTDLALPCDVSGMREPLVLGISGKPRGLQVAIGVSKEDSDDSAPSTELWPGHPETLHLDFGSAVPLWTRVNRRLFLTNCSPIQTPFTLEFEYFGSPVNSLSQKPSPPDMPPALLKTARMRELLAKREQWEFMESMLSHGKGAAFFPHLSQGVLGAHQKLSIDLTACANMWGEYWDSLICTVGGLPPAVIPVRMAVVGCPISSQRTTYYTTDQTQREPIIRFGTRVSGGDPVTRILRLNNSSPCDIRLDWETYTPEDREDRLLELLVFYGPPFPLRDHAGNDLLYPETPESSGSFWSPSPSESESSREATPSAEGSSSAGAPQKIISVILRGHEGVPSDHLFSISPKQVVVPARGSSTIHISFTPVVLGPGVLHKVECVGYALGFLSLDDEDSSDGA

Target ORF information:

RefSeq Version	XM_005222554.3
Organism	Bos taurus(cattle)
Definition	Bos taurus deleted in lung and esophageal cancer 1 (DLEC1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005222554.3

ORF Insert Sequence:

ATGGAGACTG GAAAGCGAGA GCGGCAGTCG GCTGAGTCTG GATCCTACGC TTTCGCTGGG 
GCAACGTGGA CGCCCAGCCC GCGTCTGGCC CGGATTCGCA GCCCTAGCCA GTCCACCTGG 
CTCTCCTCCC TCGCCTACTC CGAGTCCTTC CACCATGGCT TCGGGGGTCG GCGCCGCTTC 
GTCGCGCAGC TTTCGCTCCC GCCGCTCCCC GAGCCGCAAC TGCTGCGGTT GCGCCCCACC 
TCTCTGCGCA CCCAAGACAT CTCGTACTTG CTCACCGGCG TCTTCCGCAA CCTGTACACC 
GCCGAGGTGA TAGGGGAGGA GGTGAGCTCG AATTTGATCA AGGCTCGCGG CAGCGAGGAT 
GCACGGCACG AGGAGTTCGT GGACCAGCTG CAGCAGATTC GGGAGCTCTA TAAGCAGCGG 
CTGGATGAAG TTGAAATGTT GGAGAGACAC ATCATTCAGG CCCGTGCACG GGCCCTTGCG 
GAGAAAGAGC GGGCCATGCA CCAGGCCAAA GTACAGTTCC TTGAGCCGTT TATCAAGATG 
CCTCCAGTGA AGACCATCTT CAGATGGTGT GTGGACAACG AGTTGCTGCG GAAGCATCAT 
TTGATCTGCC CGAATGATTA CTACAGCGAT ACGGTGCCAT TTTGCTCTGC ACCTAAAGGT 
GTCTCTGTCC CTGGATATTC AAAACCGACG TTTAGTTCTG AGAAACGTTC CATCCCAAAG 
AAGGAGCTGA CCAAGAAGCC CGCGTGCAGG AAGATGTCCT CTCAGTCTGC AGAGGAGACA 
GACTATACTA TGGACAGCCT CACATGGGAC TCGTCTTTTA AGGCCAAGCT CAGATCCAGA 
GCAGCCATCA GGAAAACAGG TTTACCGAAA AATAAAAACT GGATGAACCA TTTACGGGTG 
CCCCAGAGGC AGTTGGAGCG GCTTCAGCTT GCCAGGATGG AGAGTCGGAA CCGCTTCCTG 
AGAAACCCTC GCTTTTTCCC TCCTAACACT CCGTTGGGGG GCAAGTCTCT TCTCTTTCCT 
CCCAAGAAGC CAGCACGGAT AGGAGCCTTC CAGGACGGAG AGCTGGAGGA GAGTTGTGCT 
GATACTCCGG TGTTTCTAGC TAAGCCCTCC ATCGGATTTT TCACAGATTA TGAAATTGGA 
CCAGTTTATG AGATGGTGAT CTCCCTGCAG AACACCACAT CCACCAGCCG CCACCTTCGA 
GTCCTCCCTC CGTCCACGCC CTACTTCGCC CTGGGACTGG GGATGTTCCC AGGAGAAGGT 
GGAATGGTGG CTCCTGGAAT GACCTGCCAG TATATTGTCC AGTTTTTTCC CGACTGCCTT 
GGGGATTTTG ATGACTTTAT TCTAGTGGAG ACCCAGTCAG CGCACACGCT CCTGATCCCC 
CTGCAGGCCC GGAGGCCGCC CCCGGTGCTG ACATTGTCGC CTGTCCTGGA CTGCGGCTAC 
TGCCTCATTG GGGGCATGAA GATAACCAGA TTCATCTGCA AAAACGTGGG CTTCAGCGTG 
GGCAAGTTCT GTATCATGCC TAAGAAGAGC TGGCCACCGC CCAGCTTCAG GGCCGTTGTC 
ACCATCGGCT TTGTTGAGCA GCCTCCCTTT GGGATCCTGC CGTCGGTGTT TGAGCTGGTT 
CCAGGGCAGG CCGTATTCGT GGAGGTCCTG TTCCTCCCAA CCAGCCTCGG AAAGGCAGAG 
CAGACCTTCG TCATCGTGTG TGACAACTGC CAGATAAAGG AGCTGGTGAC CACAGGAGTT 
GGGCAGCTGG TTGCTCTGGA TCTGATCTAT ATTTCTGGTG AGAAAAGTCA GCCGGAGCCT 
GGAGAGCTCA CAGACTTAAC AGCCCAGTAC TTCATACGCT TTGAACCTGA AAACCTTCAG 
TCCACGGCCA GCAAACTGCT GATTATTAGA AACGCCACGC ACGTGGAGCT GGCCTTCCAC 
TGGCAGATCA TGAAGCCCAA CCTGCAGTCC CTGATGCCCG GAGAAACCTA CAGCACAGAC 
AGCATCAAGT ATCACCCAGA CAGGGAGACG GCCTTCTGCA TCACCCCCAA GATGGGCGTT 
CTCAGCCCCC ACACGGACCA CGAGTTCATC CTGAGCTTCT CCCCGCAAGA GCCAAGGGAT 
TTTCACAGTG TTCTCCAGAT GGTGCTGGAG GAAGTCCCAG AGCCCGTGAG TTTGGACTTG 
GAAAACTCCA AGGACCCCTC GTACTGCATG GATGACGTGA TCGTCCTGGA AATCGAGGTG 
AAGGGCTCAG TGGAACCTTT CCAGGTTCTC CTAGAACCAT ATGCTCTCAT CATCCCTGGG 
GAGAACTATA TTGGCATAAA CGTGAAGAAG AATTTTAAGA TGTGGAACAA CAGCAAGTCC 
ACCATCAGGT ACACGTGGGG GAAGATCAGC GACTGTCACA TCATTGAGGT GGAGCCCTGC 
ACGGGGACCA TTGAACCCAG CGAGGTTGAG GATTTTGAGT TGAACTTTAC CGGGGGTGTC 
CCTGGGCCGA CGAGCCAGAA CCTGCTGTGT GAGATCAAAG ATTCTCCCAC GCCCGTGGTT 
TTGCACATCG AGGCGGCCTT TAAGGGGCCG GCCCTGGTCA TTGACGTCTC AGCCCTTCAG 
TTTGGTTTGC TGCGCCTGGG ACAGAAAGCC ACCAAGTTTA TCCAGATCCG GAACCTCAGC 
CAGCTCCCGG CCACATGGGC CATGAAGGAG AGCCAGGTCT CCCTGCAAGA AAGGCAAGAG 
GGCGTGTCTC CCTTTGACAT CGAGCCATCA AGTGGCCAAG TTCAGCCACT GGGGGAGTGC 
AGAGTGAGCA TCACCTTGGA GGCCCAGCAC TGCCAGCGTC TGCAGACCGT CCTGGAGCTG 
GAGGTGGTAG ATGGGACCTG GAGCCACCTT CCCGTGTATG CTGAGGTGCA GGAGCCCTAT 
GTGTACCTGC AGAGCAGCCA GGTGGAGGTC CCCAACCTCT ACCTGGGCGT GCCCACCAAG 
ATGGCCATCA CACTCATCAA TGGCACGCTC CTGCCCACCC GCTTCCACTG GGGCAAGCTC 
CTGGGGCTCC AAGCAGATGT CTGCACAGTG AAAGTCTCCC CGAGACGTGG CACGCTGGGC 
CCCAGCGAGG AGCGCCAGCT CAGCCTGGAG TTGACTGCGC ATACCCTGGA AGCGGTGACC 
GACCTGGCCC TCCCTTGTGA CGTGTCAGGC ATGAGGGAGC CCCTGGTTCT GGGCATCTCA 
GGGAAACCCC GGGGACTGCA GGTAGCCATC GGCGTCTCTA AGGAGGACAG TGACGACAGT 
GCCCCCAGCA CAGAGCTGTG GCCGGGCCAC CCCGAGACGC TCCACCTGGA CTTCGGCTCG 
GCAGTGCCAC TGTGGACCCG TGTGAACCGC CGGCTCTTCC TGACCAACTG CTCCCCGATA 
CAGACCCCTT TCACCCTCGA GTTCGAGTAC TTTGGGAGCC CCGTGAACAG CCTGAGCCAG 
AAACCCAGCC CCCCCGACAT GCCTCCCGCC CTGCTGAAGA CGGCACGGAT GCGGGAGCTC 
TTGGCCAAGC GCGAGCAGTG GGAGTTTATG GAAAGCATGC TGTCCCATGG GAAAGGAGCA 
GCCTTCTTCC CCCACCTCTC CCAGGGCGTG CTGGGCGCCC ACCAGAAGCT CAGCATCGAC 
CTCACTGCCT GTGCCAACAT GTGGGGGGAG TACTGGGATA GTCTCATCTG CACGGTGGGA 
GGCCTGCCTC CAGCAGTCAT CCCAGTGCGC ATGGCGGTGG TGGGCTGCCC CATCAGCTCC 
CAGAGGACCA CCTACTACAC CACTGACCAG ACCCAGAGGG AGCCCATCAT CAGGTTCGGC 
ACCCGGGTCT CTGGAGGAGA CCCAGTCACC CGGATCCTTC GCCTGAATAA TTCCAGCCCC 
TGTGACATCC GCCTGGACTG GGAGACCTAC ACTCCAGAGG ACAGGGAGGA CCGGCTGCTG 
GAGCTGCTGG TGTTCTATGG GCCGCCCTTC CCGCTACGTG ACCACGCTGG GAATGACCTC 
CTGTACCCCG AGACCCCGGA GAGCAGCGGC TCCTTCTGGT CCCCAAGTCC CTCTGAGTCT 
GAGTCCAGCC GTGAAGCCAC CCCATCTGCC GAGGGCAGCT CCAGTGCTGG CGCCCCCCAG 
AAGATCATCT CAGTCATCCT GCGAGGGCAT GAGGGGGTGC CCTCTGACCA CCTGTTCTCC 
ATCAGCCCCA AGCAGGTGGT GGTCCCTGCG CGGGGCAGCA GCACCATCCA CATCTCCTTC 
ACACCCGTGG TGCTGGGCCC TGGCGTCCTG CACAAGGTGG AGTGTGTTGG CTATGCCCTG 
GGCTTCTTGA GCTTGGACGA TGAGGACTCC AGTGATGGGG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	DLEC1
Entrez Gene ID	618746
Full Name	deleted in lung and esophageal cancer 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Mus musculus (house mouse)	Dlec1	NP_796091.2
Rattus norvegicus (Norway rat)	Dlec1	XP_006244203.1
Danio rerio (zebrafish)	dlec1	XP_683226.4
Homo sapiens (human)	DLEC1	NP_031361.2
Pan troglodytes (chimpanzee)	DLEC1	XP_003309770.1
Gallus gallus (chicken)	DLEC1	XP_003640722.2
Xenopus tropicalis (tropical clawed frog)	dlec1	XP_002938052.2
	DLEC1	XP_001085770.2
Bos taurus (cattle)	DLEC1	NP_001180127.1
Canis lupus familiaris (dog)	DLEC1	XP_005634305.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

DLEC1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

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Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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