dlec1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dlec1
Entrez Gene ID	555583
Full Name	deleted in lung and esophageal cancer 1
Synonyms	si:dkey-246l21.2
General protein information	Preferred Names deleted in lung and esophageal cancer 1 Names deleted in lung and esophageal cancer protein 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017353955.1	XP_017209444.1	deleted in lung and esophageal cancer protein 1 isoform X2
XM_678134.6	XP_683226.4	deleted in lung and esophageal cancer protein 1 isoform X1
XM_017353956.1	XP_017209445.1	deleted in lung and esophageal cancer protein 1 isoform X3
XM_017353955.2	XP_017209444.1	deleted in lung and esophageal cancer protein 1 isoform X2
XM_017353957.1	XP_017209446.1	deleted in lung and esophageal cancer protein 1 isoform X4
XM_678134.7	XP_683226.4	deleted in lung and esophageal cancer protein 1 isoform X1
XM_017353956.2	XP_017209445.1	deleted in lung and esophageal cancer protein 1 isoform X3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Dlec1	NP_796091.2
Rattus norvegicus (Norway rat)	Dlec1	XP_006244203.1
Danio rerio (zebrafish)	dlec1	XP_683226.4
Homo sapiens (human)	DLEC1	NP_031361.2
Pan troglodytes (chimpanzee)	DLEC1	XP_003309770.1
Gallus gallus (chicken)	DLEC1	XP_003640722.2
Xenopus tropicalis (tropical clawed frog)	dlec1	XP_002938052.2
	DLEC1	XP_001085770.2
Bos taurus (cattle)	DLEC1	NP_001180127.1
Canis lupus familiaris (dog)	DLEC1	XP_005634305.1

Gene Ontology

The following dlec1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dlec1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa57706	XM_017353955.2 Latest version!	Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa57707	XM_017353956.2 Latest version!	Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa57708	XM_017353957.1 Latest version!	Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa28564	XM_678134.7 Latest version!	Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa57706	XM_017353955.1	Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa28564	XM_678134.6	Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa57707	XM_017353956.1	Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dlec1 ( XM_017353955.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209444.1 Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X2, mRNA.

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CloneID	ODa57706
Clone ID Related Accession (Same CDS sequence)	XM_017353955.1 , XM_017353955.2
Accession Version	XM_017353955.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	deleted in lung and esophageal cancer protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATG AAACTGAACC AAAAGCACTA ACGTTAAAGA GTTCAGAACC GTCAATAAAC 
CGGCACACAC CAGCTTCAAA GACAACTCAG GACATTTCTC ATCTGCTGGC CAGCATATTC 
AATGATCTCT ACAGCACAGA GGTGATTGGG AAGGACACCG TTTCCAATCT CAGTAAATCA 
CGAAGAAGAG GCAACAGCTA CCATGAAAAA TATGTTGAGG AGCTACAACA GGTGCACAGT 
GAATACAACA GACGTATTCA GAATGCTGAT ATGCTAGAGA CACACATCAT ACAGGCCCGT 
CTTCAGGCTG CTGCAAAAGA AGAGCATGAT CGCAACAAAA TCCTGGAAGA AGTCGGTGAG 
GCTTACCAAC AGCTGGGACT TCCCCCAGTA AAATCAGCTT TCAAGTGGTG TGTGGACAGT 
GGACTTTTGA GGAGCAACAG CTTGATTTGC CCACTGGACT ACACAACAGT GCACAACCCA 
GTTGTTAAAA GCCCAAAAGC CAGGTCAAGT CCAGGCTTTG CCAAGCCTAC TTTCTCTTAT 
AATATGCACA TCTCCACTGA ACTGCAGGAT GATGGTTACA CTCCGCTTCC TCCACACGCT 
CATTCAGCAC ACAGCCTGCT TGAATATTCA GAGGAGACAA TCACACTCTC TTCCTCTCCA 
GAGTCCAGCT CCATCAATGA CACAGAGCTA GGTTTGGTGG CGAAGGCTTC TTGGATGGAG 
AAGACCAGTG CTAAGAGTCA TGCAGAAGAC AGGGCTTCCC TGAAAAAGCA CAAAGACCGG 
CATAAGTTTC TGCACAACCC TCGCTTCTTG CTGCCAAACA CTCAGCATGG AGGAAAGTCT 
CTCATCATCC CAGGGAAAAA GAAGCTGGAG AATGTGGGAA AGAGCAGGAA AAACAACGGC 
AGAGAGAGTC TTGAGGGTCA TGCGCCTATT TTCACTGCAA ATCCACCAGT GATCTTCTTC 
AGTGACTACA GAGTAGGACA AGTCTATGAG ACCTCAGTGG AGCTCAAAAA CATGACTGCG 
AGCAGCCGTC ACATTCGAAT GATTCCTCCA ACCTCCCCAC ACTTCTCTGT GGGTTTGGGC 
CGGTTCCCTG GTGAAGGAGG CATCGTGGCT CCAGGAATGA GCTGTCAGTA TATGGTGCGT 
TTTGCTCCAG ATTCTCTAGC AGACTATGAA GACGCTCTGG TTGTGGAGAC GCAGATGCTC 
TATCCTCTCA TTATACCAAT AGAAGCCTGC AGATCTCCTC CGATACTCAC CTTGCCGGCT 
GTCATGGACT GTGGTTTCTG CCTGGTGGGA GGAGTGAAGT TTATGGAGGT TTTATGTCGG 
AATGAAGGAC TGAGTGCTGG AACGTTTTGT GTAATGCCTA AGAAACAGTG GCCTGCTTCA 
AGCCTCAGGT CTGCAGTGAA GTCGACTTTC ACAGAACAGC CTCCCTTTGC TATCAGTCCC 
TCTATTTTCG GCCTTCACCC TGGACAGGCT ACTGTCATAG AGGTTGTGTT TTTCCCCACA 
ACTCCTGAGC TCTCTACTCA AGACTTCACC ATTGTCTGTG ACAACTGCCA AGTGAAGGAC 
ATTACGCTCC AAGGTACTGG TCAACTGATC ACTGTGGAGC TGCTTTCAGT ATCAGGTGGA 
GAGGATCAGC CTGAATTTGC AGAATTGTGT GACATCACAG CTGACCATTA TGTCAGATTT 
AACACAACCA ATCCACACTC TACCCTGCAG AAGACACTGG TTGTCAAAAA CAACACACAT 
TTGGAGCTTC CATATCACTG GCAGATCATG AAGCCCAGTC TTCAATGCTT GTTGCCCGGA 
GAGACTCCTG ACCCTTCCAG CATTCAGCAT CTTCTTGATA CAGACAGTGC ATTCAGCATC 
AGTCCTGTTA TAGGAATCCT GAACCCTGCA CAGGAACACG AGTTCCTGCT CACCTATGCT 
CCCAAAGATA TAAAAGATTA CCACAGTGTC TGCCACTTGG TGATTATGGA TGTTCCCAGT 
CCACCTAAAG TCTGTGACGA TGGGACATGT CCTGATACTG CACTCCATGC AAACAATGTG 
ACCATTTTAG AGGTTGAAGT GAAAGGTTCT ACTGAGCCAT ACAAGATCCT TCTTGAGCCG 
TATGCTGTTT TAATACCAGG AGAGACCTAT ATACACAACA CAATTTGCAA GAGATTCAAG 
ATGTGGAATC ACAGCAAATC AGTCATTCAC TTTGAATGGG AACGTGTTAT TGACAGTCAT 
GTAATTGAAG TGGAGCCCTC ATCTGGAGTA ATAGAAATGA ATGAGTGTTT TGACATGGAG 
TTGTATCTGA CTGGAAGGAG TCCGGGTCAT TTTACCTCTA CCCTGCAGTG TCACATTCTG 
CACCATCCTA AAACTATAGG ACTTGCCATT GAGGCCACAT TTAAGGGTCC ACATTGCTCA 
TTGAATGTAC CCAGTCTGGA TTTTGGTTTG CTGCAAATGG GAAATGAGAG AATTTTGACT 
ATTGAAGTCA CCAACAACAG CCTGCTAGAT GCCCACTGGA GCCTGGAGGT GCTGTCCCAA 
ACCCAGAAGC TCATCGATGG ACAGGTGCAT ATAAATCCAA ATAAAGGTGT TCTGGCTGCA 
CTAGAGAGCT GCAGTGTGGA TGTGGCCTTC AGAGCCATGC ATTGCCAAGT ATTTGAGTCA 
GTTTTACAAC TTTCTGTGCT GAACGGCACC GGTTGTCACG TCTCCATCCA TGCCGTTGTT 
CAGTCTCCTC AAGTGTGCTT GCTGAACTGT GAGTTGCTGT TTGCTGATCT GTATGTTGGG 
GTTTCACAGA CGGGCAGTGT CACTCTGTTT AACCAAACAC TACTGCCAGC TCACTTCACC 
TGGGGCAGGC TTCAAGGTTC ACAAGCTCAT CTCTGCTCCG CAGTCTTCAG TCCCTCTGCA 
GGATCTCTGG GGCCCAATGC AAAAACTGAA GTCTCTGTGT CATTCACAGC TCACACTGTT 
GAGGATTTGA CTGAAGTGAA TGGAGTTTGT GAGGTAAAAG GAATGGAGAA TCCACTTGTG 
CTGGGAATTT TTTCTAAAGC CAAACCTCTC AGAGTTTCCT ACTCACTGCT TTGTAATGGT 
ATTAATTCAG GTGATGGCGA TCACCAACCA ATCCTGCTGG ACTTCACTGA GCATGAGCCA 
GCCGTCATTG GGAAATCTGT CAAACGGCAG TTACTGATAA CTAACAACAC TGCTATTACT 
GCTCCATTCA CTGTCGACAC TGAGTACTTC ACTGGAGACT GTCCTTCACA ATCAGAAGGA 
AACTCTGAAA GAAGCAGTGC ACCTTTCCAC TCCATCCAGG CCAAAAAAAT ACAGCAAAAG 
GCATATGATG AGTTTGTAAG CCGTCTGCTT TCCCATGGAA GAGGTGCAAC ATTTTATGTA 
GAACCAAAAA GTGGGATGCT TGGCCCATTC GAGAGTCAAA CCATCAACAT CACTACTTTC 
ACAAATATGT GGGGGGATTA TCAGGACAAC CTCATATGCA AAGTTGGAGA TCTAAATCCA 
ACTCACATTC ATATGAAGAT GTCTGTGAGG GGCTGTCCAG TTTACTTTCA GATGATTGGA 
CCACAGCCTG ACAATCAGAA TCAGGGTCCA GTTATTAGAT TTGGAAGCCA TGTTTCTGGA 
GGAGACACTG TGTCACGCTC TTTACGACTC ATTAACACCA GTCCATATGA TATCCGTATG 
GACTGGGTGA CCTACAACCT GGAGGCTGGA GACGGCAAGC TGATAGATCT GCTGCTTGCA 
TGTGGTGAAC CTTTCCCTCT GAAGGATGCA GATGGCAATG AGGTTGTTGG AGGACTGGAC 
TCATCTGTCA TGTTCCCGTC CACATGGGAT GACAGTCACA CCCCCAGCAG AGAAGGAACA 
AGCTCAACTT TTATGACCCA ATCTGATGAT TTTGTAGAGA ATGGTGAAGA ATGTGATGGA 
GAAGATGGCA GTGGGGCTTC TGAGTCTCTA GCTCCAGTGA AGAAGCTGTT TTCTGTGTTT 
TTGAAGCCTC ATGAAGGGAC TCTTTCTGAC TACCCATACT GCATCACTCC ACAGCAGACT 
GTAGTTCCAG CAGGGGGCAG TAGCACCATT CATTTGTCTT TCACTCCCCT GATTCTGTCA 
TGTTCAACCA ATCAAAGATG TTTGGGTTAC GCTCTTGGTT TCATGAGCTT AGACTCTAAG 
CTTGCGTTAG TGACTCCAGG CAAGGTGGAG AGAGTCCGGG GCTATGAACT TCAACCTTTG 
AGGCTGGACA TGCAGGCTAA TGTAAAACCT GCCATTTTGT CAGTGCAGAT GGCAGAGGAT 
ACAGAAGTGC TGGAATTCAG CGCAGCTGCC AGCGACACAC TAGACGGAGC ATCACAAACA 
CAGGAATCCA GGATAACTCG CACATTCCGG CTAAAGAACG ACACAGACAT GGCCTTGAGT 
TTCAAACTGA GCACAAATCC ACCATTTTCA GTGCTGCTGC CCCGACAGAG CCTTAAACTG 
ATCAGCAGCT CGCCTGCTTA TAGACACACA CAAGGTCTGT CCGGACCAGG AGATGAGATG 
AGATTATTTC TGTTACAGCC CAAAAACATC ATTCAGGTTA AGGTGGCTTT CCACAATTCC 
GCTTCACTCC TGGCCTGTGT AAATGGAGCA TGTGAGGAGT CGGACAGTCA GCTCTCTGCT 
TCCCTTCTTT GCAGTGATGA TGGAGGAAAA ACTCTACAGT TTCAGGAGAA CTTGACCATT 
CAGTACAGCA ATAACAGCAT ACAGACAGTC TCTCTTTACG CTCGTCTGGC ATTACCTACA 
CTACACCTGT CCAGTGCCAC TGTGGATTTT GGCACCTGTT ATGTTGGACA AACAACTGTC 
AAAGAGATTT ATCTTCACAA CAGAGGTGGC TCCTGTAGCT ACTGGACCAC ACTCACAGAT 
GGCAATGAAG TGTTCAGGGT AAGTCCGGAT TCTGGTTTAC TGAAACCTCT TGGAGATCCA 
GCGTCCTGTA GACAGCTGCT GCAGATCAGC TTCACTCCGA GAGAACAGAA AGAGTTCCAG 
ACAATCTTCA CAGTGCAGGG GCTTCTTGGA GAGACACCAC TTGTTCTAAA GGTCAGCGGA 
AGAGGATCAT TTGATGAGAG TTTTGTATCT CCAATAACTG ACAGCTCACA AAACAACACT 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209444.1
CDS	180..5285
Translation	MADETEPKALTLKSSEPSINRHTPASKTTQDISHLLASIFNDLYSTEVIGKDTVSNLSKSRRRGNSYHEKYVEELQQVHSEYNRRIQNADMLETHIIQARLQAAAKEEHDRNKILEEVGEAYQQLGLPPVKSAFKWCVDSGLLRSNSLICPLDYTTVHNPVVKSPKARSSPGFAKPTFSYNMHISTELQDDGYTPLPPHAHSAHSLLEYSEETITLSSSPESSSINDTELGLVAKASWMEKTSAKSHAEDRASLKKHKDRHKFLHNPRFLLPNTQHGGKSLIIPGKKKLENVGKSRKNNGRESLEGHAPIFTANPPVIFFSDYRVGQVYETSVELKNMTASSRHIRMIPPTSPHFSVGLGRFPGEGGIVAPGMSCQYMVRFAPDSLADYEDALVVETQMLYPLIIPIEACRSPPILTLPAVMDCGFCLVGGVKFMEVLCRNEGLSAGTFCVMPKKQWPASSLRSAVKSTFTEQPPFAISPSIFGLHPGQATVIEVVFFPTTPELSTQDFTIVCDNCQVKDITLQGTGQLITVELLSVSGGEDQPEFAELCDITADHYVRFNTTNPHSTLQKTLVVKNNTHLELPYHWQIMKPSLQCLLPGETPDPSSIQHLLDTDSAFSISPVIGILNPAQEHEFLLTYAPKDIKDYHSVCHLVIMDVPSPPKVCDDGTCPDTALHANNVTILEVEVKGSTEPYKILLEPYAVLIPGETYIHNTICKRFKMWNHSKSVIHFEWERVIDSHVIEVEPSSGVIEMNECFDMELYLTGRSPGHFTSTLQCHILHHPKTIGLAIEATFKGPHCSLNVPSLDFGLLQMGNERILTIEVTNNSLLDAHWSLEVLSQTQKLIDGQVHINPNKGVLAALESCSVDVAFRAMHCQVFESVLQLSVLNGTGCHVSIHAVVQSPQVCLLNCELLFADLYVGVSQTGSVTLFNQTLLPAHFTWGRLQGSQAHLCSAVFSPSAGSLGPNAKTEVSVSFTAHTVEDLTEVNGVCEVKGMENPLVLGIFSKAKPLRVSYSLLCNGINSGDGDHQPILLDFTEHEPAVIGKSVKRQLLITNNTAITAPFTVDTEYFTGDCPSQSEGNSERSSAPFHSIQAKKIQQKAYDEFVSRLLSHGRGATFYVEPKSGMLGPFESQTINITTFTNMWGDYQDNLICKVGDLNPTHIHMKMSVRGCPVYFQMIGPQPDNQNQGPVIRFGSHVSGGDTVSRSLRLINTSPYDIRMDWVTYNLEAGDGKLIDLLLACGEPFPLKDADGNEVVGGLDSSVMFPSTWDDSHTPSREGTSSTFMTQSDDFVENGEECDGEDGSGASESLAPVKKLFSVFLKPHEGTLSDYPYCITPQQTVVPAGGSSTIHLSFTPLILSCSTNQRCLGYALGFMSLDSKLALVTPGKVERVRGYELQPLRLDMQANVKPAILSVQMAEDTEVLEFSAAASDTLDGASQTQESRITRTFRLKNDTDMALSFKLSTNPPFSVLLPRQSLKLISSSPAYRHTQGLSGPGDEMRLFLLQPKNIIQVKVAFHNSASLLACVNGACEESDSQLSASLLCSDDGGKTLQFQENLTIQYSNNSIQTVSLYARLALPTLHLSSATVDFGTCYVGQTTVKEIYLHNRGGSCSYWTTLTDGNEVFRVSPDSGLLKPLGDPASCRQLLQISFTPREQKEFQTIFTVQGLLGETPLVLKVSGRGSFDESFVSPITDSSQNNTE

Target ORF information:

RefSeq Version	XM_017353955.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353955.1

ORF Insert Sequence:

ATGGCTGATG AAACTGAACC AAAAGCACTA ACGTTAAAGA GTTCAGAACC GTCAATAAAC 
CGGCACACAC CAGCTTCAAA GACAACTCAG GACATTTCTC ATCTGCTGGC CAGCATATTC 
AATGATCTCT ACAGCACAGA GGTGATTGGG AAGGACACCG TTTCCAATCT CAGTAAATCA 
CGAAGAAGAG GCAACAGCTA CCATGAAAAA TATGTTGAGG AGCTACAACA GGTGCACAGT 
GAATACAACA GACGTATTCA GAATGCTGAT ATGCTAGAGA CACACATCAT ACAGGCCCGT 
CTTCAGGCTG CTGCAAAAGA AGAGCATGAT CGCAACAAAA TCCTGGAAGA AGTCGGTGAG 
GCTTACCAAC AGCTGGGACT TCCCCCAGTA AAATCAGCTT TCAAGTGGTG TGTGGACAGT 
GGACTTTTGA GGAGCAACAG CTTGATTTGC CCACTGGACT ACACAACAGT GCACAACCCA 
GTTGTTAAAA GCCCAAAAGC CAGGTCAAGT CCAGGCTTTG CCAAGCCTAC TTTCTCTTAT 
AATATGCACA TCTCCACTGA ACTGCAGGAT GATGGTTACA CTCCGCTTCC TCCACACGCT 
CATTCAGCAC ACAGCCTGCT TGAATATTCA GAGGAGACAA TCACACTCTC TTCCTCTCCA 
GAGTCCAGCT CCATCAATGA CACAGAGCTA GGTTTGGTGG CGAAGGCTTC TTGGATGGAG 
AAGACCAGTG CTAAGAGTCA TGCAGAAGAC AGGGCTTCCC TGAAAAAGCA CAAAGACCGG 
CATAAGTTTC TGCACAACCC TCGCTTCTTG CTGCCAAACA CTCAGCATGG AGGAAAGTCT 
CTCATCATCC CAGGGAAAAA GAAGCTGGAG AATGTGGGAA AGAGCAGGAA AAACAACGGC 
AGAGAGAGTC TTGAGGGTCA TGCGCCTATT TTCACTGCAA ATCCACCAGT GATCTTCTTC 
AGTGACTACA GAGTAGGACA AGTCTATGAG ACCTCAGTGG AGCTCAAAAA CATGACTGCG 
AGCAGCCGTC ACATTCGAAT GATTCCTCCA ACCTCCCCAC ACTTCTCTGT GGGTTTGGGC 
CGGTTCCCTG GTGAAGGAGG CATCGTGGCT CCAGGAATGA GCTGTCAGTA TATGGTGCGT 
TTTGCTCCAG ATTCTCTAGC AGACTATGAA GACGCTCTGG TTGTGGAGAC GCAGATGCTC 
TATCCTCTCA TTATACCAAT AGAAGCCTGC AGATCTCCTC CGATACTCAC CTTGCCGGCT 
GTCATGGACT GTGGTTTCTG CCTGGTGGGA GGAGTGAAGT TTATGGAGGT TTTATGTCGG 
AATGAAGGAC TGAGTGCTGG AACGTTTTGT GTAATGCCTA AGAAACAGTG GCCTGCTTCA 
AGCCTCAGGT CTGCAGTGAA GTCGACTTTC ACAGAACAGC CTCCCTTTGC TATCAGTCCC 
TCTATTTTCG GCCTTCACCC TGGACAGGCT ACTGTCATAG AGGTTGTGTT TTTCCCCACA 
ACTCCTGAGC TCTCTACTCA AGACTTCACC ATTGTCTGTG ACAACTGCCA AGTGAAGGAC 
ATTACGCTCC AAGGTACTGG TCAACTGATC ACTGTGGAGC TGCTTTCAGT ATCAGGTGGA 
GAGGATCAGC CTGAATTTGC AGAATTGTGT GACATCACAG CTGACCATTA TGTCAGATTT 
AACACAACCA ATCCACACTC TACCCTGCAG AAGACACTGG TTGTCAAAAA CAACACACAT 
TTGGAGCTTC CATATCACTG GCAGATCATG AAGCCCAGTC TTCAATGCTT GTTGCCCGGA 
GAGACTCCTG ACCCTTCCAG CATTCAGCAT CTTCTTGATA CAGACAGTGC ATTCAGCATC 
AGTCCTGTTA TAGGAATCCT GAACCCTGCA CAGGAACACG AGTTCCTGCT CACCTATGCT 
CCCAAAGATA TAAAAGATTA CCACAGTGTC TGCCACTTGG TGATTATGGA TGTTCCCAGT 
CCACCTAAAG TCTGTGACGA TGGGACATGT CCTGATACTG CACTCCATGC AAACAATGTG 
ACCATTTTAG AGGTTGAAGT GAAAGGTTCT ACTGAGCCAT ACAAGATCCT TCTTGAGCCG 
TATGCTGTTT TAATACCAGG AGAGACCTAT ATACACAACA CAATTTGCAA GAGATTCAAG 
ATGTGGAATC ACAGCAAATC AGTCATTCAC TTTGAATGGG AACGTGTTAT TGACAGTCAT 
GTAATTGAAG TGGAGCCCTC ATCTGGAGTA ATAGAAATGA ATGAGTGTTT TGACATGGAG 
TTGTATCTGA CTGGAAGGAG TCCGGGTCAT TTTACCTCTA CCCTGCAGTG TCACATTCTG 
CACCATCCTA AAACTATAGG ACTTGCCATT GAGGCCACAT TTAAGGGTCC ACATTGCTCA 
TTGAATGTAC CCAGTCTGGA TTTTGGTTTG CTGCAAATGG GAAATGAGAG AATTTTGACT 
ATTGAAGTCA CCAACAACAG CCTGCTAGAT GCCCACTGGA GCCTGGAGGT GCTGTCCCAA 
ACCCAGAAGC TCATCGATGG ACAGGTGCAT ATAAATCCAA ATAAAGGTGT TCTGGCTGCA 
CTAGAGAGCT GCAGTGTGGA TGTGGCCTTC AGAGCCATGC ATTGCCAAGT ATTTGAGTCA 
GTTTTACAAC TTTCTGTGCT GAACGGCACC GGTTGTCACG TCTCCATCCA TGCCGTTGTT 
CAGTCTCCTC AAGTGTGCTT GCTGAACTGT GAGTTGCTGT TTGCTGATCT GTATGTTGGG 
GTTTCACAGA CGGGCAGTGT CACTCTGTTT AACCAAACAC TACTGCCAGC TCACTTCACC 
TGGGGCAGGC TTCAAGGTTC ACAAGCTCAT CTCTGCTCCG CAGTCTTCAG TCCCTCTGCA 
GGATCTCTGG GGCCCAATGC AAAAACTGAA GTCTCTGTGT CATTCACAGC TCACACTGTT 
GAGGATTTGA CTGAAGTGAA TGGAGTTTGT GAGGTAAAAG GAATGGAGAA TCCACTTGTG 
CTGGGAATTT TTTCTAAAGC CAAACCTCTC AGAGTTTCCT ACTCACTGCT TTGTAATGGT 
ATTAATTCAG GTGATGGCGA TCACCAACCA ATCCTGCTGG ACTTCACTGA GCATGAGCCA 
GCCGTCATTG GGAAATCTGT CAAACGGCAG TTACTGATAA CTAACAACAC TGCTATTACT 
GCTCCATTCA CTGTCGACAC TGAGTACTTC ACTGGAGACT GTCCTTCACA ATCAGAAGGA 
AACTCTGAAA GAAGCAGTGC ACCTTTCCAC TCCATCCAGG CCAAAAAAAT ACAGCAAAAG 
GCATATGATG AGTTTGTAAG CCGTCTGCTT TCCCATGGAA GAGGTGCAAC ATTTTATGTA 
GAACCAAAAA GTGGGATGCT TGGCCCATTC GAGAGTCAAA CCATCAACAT CACTACTTTC 
ACAAATATGT GGGGGGATTA TCAGGACAAC CTCATATGCA AAGTTGGAGA TCTAAATCCA 
ACTCACATTC ATATGAAGAT GTCTGTGAGG GGCTGTCCAG TTTACTTTCA GATGATTGGA 
CCACAGCCTG ACAATCAGAA TCAGGGTCCA GTTATTAGAT TTGGAAGCCA TGTTTCTGGA 
GGAGACACTG TGTCACGCTC TTTACGACTC ATTAACACCA GTCCATATGA TATCCGTATG 
GACTGGGTGA CCTACAACCT GGAGGCTGGA GACGGCAAGC TGATAGATCT GCTGCTTGCA 
TGTGGTGAAC CTTTCCCTCT GAAGGATGCA GATGGCAATG AGGTTGTTGG AGGACTGGAC 
TCATCTGTCA TGTTCCCGTC CACATGGGAT GACAGTCACA CCCCCAGCAG AGAAGGAACA 
AGCTCAACTT TTATGACCCA ATCTGATGAT TTTGTAGAGA ATGGTGAAGA ATGTGATGGA 
GAAGATGGCA GTGGGGCTTC TGAGTCTCTA GCTCCAGTGA AGAAGCTGTT TTCTGTGTTT 
TTGAAGCCTC ATGAAGGGAC TCTTTCTGAC TACCCATACT GCATCACTCC ACAGCAGACT 
GTAGTTCCAG CAGGGGGCAG TAGCACCATT CATTTGTCTT TCACTCCCCT GATTCTGTCA 
TGTTCAACCA ATCAAAGATG TTTGGGTTAC GCTCTTGGTT TCATGAGCTT AGACTCTAAG 
CTTGCGTTAG TGACTCCAGG CAAGGTGGAG AGAGTCCGGG GCTATGAACT TCAACCTTTG 
AGGCTGGACA TGCAGGCTAA TGTAAAACCT GCCATTTTGT CAGTGCAGAT GGCAGAGGAT 
ACAGAAGTGC TGGAATTCAG CGCAGCTGCC AGCGACACAC TAGACGGAGC ATCACAAACA 
CAGGAATCCA GGATAACTCG CACATTCCGG CTAAAGAACG ACACAGACAT GGCCTTGAGT 
TTCAAACTGA GCACAAATCC ACCATTTTCA GTGCTGCTGC CCCGACAGAG CCTTAAACTG 
ATCAGCAGCT CGCCTGCTTA TAGACACACA CAAGGTCTGT CCGGACCAGG AGATGAGATG 
AGATTATTTC TGTTACAGCC CAAAAACATC ATTCAGGTTA AGGTGGCTTT CCACAATTCC 
GCTTCACTCC TGGCCTGTGT AAATGGAGCA TGTGAGGAGT CGGACAGTCA GCTCTCTGCT 
TCCCTTCTTT GCAGTGATGA TGGAGGAAAA ACTCTACAGT TTCAGGAGAA CTTGACCATT 
CAGTACAGCA ATAACAGCAT ACAGACAGTC TCTCTTTACG CTCGTCTGGC ATTACCTACA 
CTACACCTGT CCAGTGCCAC TGTGGATTTT GGCACCTGTT ATGTTGGACA AACAACTGTC 
AAAGAGATTT ATCTTCACAA CAGAGGTGGC TCCTGTAGCT ACTGGACCAC ACTCACAGAT 
GGCAATGAAG TGTTCAGGGT AAGTCCGGAT TCTGGTTTAC TGAAACCTCT TGGAGATCCA 
GCGTCCTGTA GACAGCTGCT GCAGATCAGC TTCACTCCGA GAGAACAGAA AGAGTTCCAG 
ACAATCTTCA CAGTGCAGGG GCTTCTTGGA GAGACACCAC TTGTTCTAAA GGTCAGCGGA 
AGAGGATCAT TTGATGAGAG TTTTGTATCT CCAATAACTG ACAGCTCACA AAACAACACT 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlec1 ( XM_017353955.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209444.1 Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X2, mRNA.

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CloneID	ODa57706
Clone ID Related Accession (Same CDS sequence)	XM_017353955.1 , XM_017353955.2
Accession Version	XM_017353955.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	deleted in lung and esophageal cancer protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353955.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATG AAACTGAACC AAAAGCACTA ACGTTAAAGA GTTCAGAACC GTCAATAAAC 
CGGCACACAC CAGCTTCAAA GACAACTCAG GACATTTCTC ATCTGCTGGC CAGCATATTC 
AATGATCTCT ACAGCACAGA GGTGATTGGG AAGGACACCG TTTCCAATCT CAGTAAATCA 
CGAAGAAGAG GCAACAGCTA CCATGAAAAA TATGTTGAGG AGCTACAACA GGTGCACAGT 
GAATACAACA GACGTATTCA GAATGCTGAT ATGCTAGAGA CACACATCAT ACAGGCCCGT 
CTTCAGGCTG CTGCAAAAGA AGAGCATGAT CGCAACAAAA TCCTGGAAGA AGTCGGTGAG 
GCTTACCAAC AGCTGGGACT TCCCCCAGTA AAATCAGCTT TCAAGTGGTG TGTGGACAGT 
GGACTTTTGA GGAGCAACAG CTTGATTTGC CCACTGGACT ACACAACAGT GCACAACCCA 
GTTGTTAAAA GCCCAAAAGC CAGGTCAAGT CCAGGCTTTG CCAAGCCTAC TTTCTCTTAT 
AATATGCACA TCTCCACTGA ACTGCAGGAT GATGGTTACA CTCCGCTTCC TCCACACGCT 
CATTCAGCAC ACAGCCTGCT TGAATATTCA GAGGAGACAA TCACACTCTC TTCCTCTCCA 
GAGTCCAGCT CCATCAATGA CACAGAGCTA GGTTTGGTGG CGAAGGCTTC TTGGATGGAG 
AAGACCAGTG CTAAGAGTCA TGCAGAAGAC AGGGCTTCCC TGAAAAAGCA CAAAGACCGG 
CATAAGTTTC TGCACAACCC TCGCTTCTTG CTGCCAAACA CTCAGCATGG AGGAAAGTCT 
CTCATCATCC CAGGGAAAAA GAAGCTGGAG AATGTGGGAA AGAGCAGGAA AAACAACGGC 
AGAGAGAGTC TTGAGGGTCA TGCGCCTATT TTCACTGCAA ATCCACCAGT GATCTTCTTC 
AGTGACTACA GAGTAGGACA AGTCTATGAG ACCTCAGTGG AGCTCAAAAA CATGACTGCG 
AGCAGCCGTC ACATTCGAAT GATTCCTCCA ACCTCCCCAC ACTTCTCTGT GGGTTTGGGC 
CGGTTCCCTG GTGAAGGAGG CATCGTGGCT CCAGGAATGA GCTGTCAGTA TATGGTGCGT 
TTTGCTCCAG ATTCTCTAGC AGACTATGAA GACGCTCTGG TTGTGGAGAC GCAGATGCTC 
TATCCTCTCA TTATACCAAT AGAAGCCTGC AGATCTCCTC CGATACTCAC CTTGCCGGCT 
GTCATGGACT GTGGTTTCTG CCTGGTGGGA GGAGTGAAGT TTATGGAGGT TTTATGTCGG 
AATGAAGGAC TGAGTGCTGG AACGTTTTGT GTAATGCCTA AGAAACAGTG GCCTGCTTCA 
AGCCTCAGGT CTGCAGTGAA GTCGACTTTC ACAGAACAGC CTCCCTTTGC TATCAGTCCC 
TCTATTTTCG GCCTTCACCC TGGACAGGCT ACTGTCATAG AGGTTGTGTT TTTCCCCACA 
ACTCCTGAGC TCTCTACTCA AGACTTCACC ATTGTCTGTG ACAACTGCCA AGTGAAGGAC 
ATTACGCTCC AAGGTACTGG TCAACTGATC ACTGTGGAGC TGCTTTCAGT ATCAGGTGGA 
GAGGATCAGC CTGAATTTGC AGAATTGTGT GACATCACAG CTGACCATTA TGTCAGATTT 
AACACAACCA ATCCACACTC TACCCTGCAG AAGACACTGG TTGTCAAAAA CAACACACAT 
TTGGAGCTTC CATATCACTG GCAGATCATG AAGCCCAGTC TTCAATGCTT GTTGCCCGGA 
GAGACTCCTG ACCCTTCCAG CATTCAGCAT CTTCTTGATA CAGACAGTGC ATTCAGCATC 
AGTCCTGTTA TAGGAATCCT GAACCCTGCA CAGGAACACG AGTTCCTGCT CACCTATGCT 
CCCAAAGATA TAAAAGATTA CCACAGTGTC TGCCACTTGG TGATTATGGA TGTTCCCAGT 
CCACCTAAAG TCTGTGACGA TGGGACATGT CCTGATACTG CACTCCATGC AAACAATGTG 
ACCATTTTAG AGGTTGAAGT GAAAGGTTCT ACTGAGCCAT ACAAGATCCT TCTTGAGCCG 
TATGCTGTTT TAATACCAGG AGAGACCTAT ATACACAACA CAATTTGCAA GAGATTCAAG 
ATGTGGAATC ACAGCAAATC AGTCATTCAC TTTGAATGGG AACGTGTTAT TGACAGTCAT 
GTAATTGAAG TGGAGCCCTC ATCTGGAGTA ATAGAAATGA ATGAGTGTTT TGACATGGAG 
TTGTATCTGA CTGGAAGGAG TCCGGGTCAT TTTACCTCTA CCCTGCAGTG TCACATTCTG 
CACCATCCTA AAACTATAGG ACTTGCCATT GAGGCCACAT TTAAGGGTCC ACATTGCTCA 
TTGAATGTAC CCAGTCTGGA TTTTGGTTTG CTGCAAATGG GAAATGAGAG AATTTTGACT 
ATTGAAGTCA CCAACAACAG CCTGCTAGAT GCCCACTGGA GCCTGGAGGT GCTGTCCCAA 
ACCCAGAAGC TCATCGATGG ACAGGTGCAT ATAAATCCAA ATAAAGGTGT TCTGGCTGCA 
CTAGAGAGCT GCAGTGTGGA TGTGGCCTTC AGAGCCATGC ATTGCCAAGT ATTTGAGTCA 
GTTTTACAAC TTTCTGTGCT GAACGGCACC GGTTGTCACG TCTCCATCCA TGCCGTTGTT 
CAGTCTCCTC AAGTGTGCTT GCTGAACTGT GAGTTGCTGT TTGCTGATCT GTATGTTGGG 
GTTTCACAGA CGGGCAGTGT CACTCTGTTT AACCAAACAC TACTGCCAGC TCACTTCACC 
TGGGGCAGGC TTCAAGGTTC ACAAGCTCAT CTCTGCTCCG CAGTCTTCAG TCCCTCTGCA 
GGATCTCTGG GGCCCAATGC AAAAACTGAA GTCTCTGTGT CATTCACAGC TCACACTGTT 
GAGGATTTGA CTGAAGTGAA TGGAGTTTGT GAGGTAAAAG GAATGGAGAA TCCACTTGTG 
CTGGGAATTT TTTCTAAAGC CAAACCTCTC AGAGTTTCCT ACTCACTGCT TTGTAATGGT 
ATTAATTCAG GTGATGGCGA TCACCAACCA ATCCTGCTGG ACTTCACTGA GCATGAGCCA 
GCCGTCATTG GGAAATCTGT CAAACGGCAG TTACTGATAA CTAACAACAC TGCTATTACT 
GCTCCATTCA CTGTCGACAC TGAGTACTTC ACTGGAGACT GTCCTTCACA ATCAGAAGGA 
AACTCTGAAA GAAGCAGTGC ACCTTTCCAC TCCATCCAGG CCAAAAAAAT ACAGCAAAAG 
GCATATGATG AGTTTGTAAG CCGTCTGCTT TCCCATGGAA GAGGTGCAAC ATTTTATGTA 
GAACCAAAAA GTGGGATGCT TGGCCCATTC GAGAGTCAAA CCATCAACAT CACTACTTTC 
ACAAATATGT GGGGGGATTA TCAGGACAAC CTCATATGCA AAGTTGGAGA TCTAAATCCA 
ACTCACATTC ATATGAAGAT GTCTGTGAGG GGCTGTCCAG TTTACTTTCA GATGATTGGA 
CCACAGCCTG ACAATCAGAA TCAGGGTCCA GTTATTAGAT TTGGAAGCCA TGTTTCTGGA 
GGAGACACTG TGTCACGCTC TTTACGACTC ATTAACACCA GTCCATATGA TATCCGTATG 
GACTGGGTGA CCTACAACCT GGAGGCTGGA GACGGCAAGC TGATAGATCT GCTGCTTGCA 
TGTGGTGAAC CTTTCCCTCT GAAGGATGCA GATGGCAATG AGGTTGTTGG AGGACTGGAC 
TCATCTGTCA TGTTCCCGTC CACATGGGAT GACAGTCACA CCCCCAGCAG AGAAGGAACA 
AGCTCAACTT TTATGACCCA ATCTGATGAT TTTGTAGAGA ATGGTGAAGA ATGTGATGGA 
GAAGATGGCA GTGGGGCTTC TGAGTCTCTA GCTCCAGTGA AGAAGCTGTT TTCTGTGTTT 
TTGAAGCCTC ATGAAGGGAC TCTTTCTGAC TACCCATACT GCATCACTCC ACAGCAGACT 
GTAGTTCCAG CAGGGGGCAG TAGCACCATT CATTTGTCTT TCACTCCCCT GATTCTGTCA 
TGTTCAACCA ATCAAAGATG TTTGGGTTAC GCTCTTGGTT TCATGAGCTT AGACTCTAAG 
CTTGCGTTAG TGACTCCAGG CAAGGTGGAG AGAGTCCGGG GCTATGAACT TCAACCTTTG 
AGGCTGGACA TGCAGGCTAA TGTAAAACCT GCCATTTTGT CAGTGCAGAT GGCAGAGGAT 
ACAGAAGTGC TGGAATTCAG CGCAGCTGCC AGCGACACAC TAGACGGAGC ATCACAAACA 
CAGGAATCCA GGATAACTCG CACATTCCGG CTAAAGAACG ACACAGACAT GGCCTTGAGT 
TTCAAACTGA GCACAAATCC ACCATTTTCA GTGCTGCTGC CCCGACAGAG CCTTAAACTG 
ATCAGCAGCT CGCCTGCTTA TAGACACACA CAAGGTCTGT CCGGACCAGG AGATGAGATG 
AGATTATTTC TGTTACAGCC CAAAAACATC ATTCAGGTTA AGGTGGCTTT CCACAATTCC 
GCTTCACTCC TGGCCTGTGT AAATGGAGCA TGTGAGGAGT CGGACAGTCA GCTCTCTGCT 
TCCCTTCTTT GCAGTGATGA TGGAGGAAAA ACTCTACAGT TTCAGGAGAA CTTGACCATT 
CAGTACAGCA ATAACAGCAT ACAGACAGTC TCTCTTTACG CTCGTCTGGC ATTACCTACA 
CTACACCTGT CCAGTGCCAC TGTGGATTTT GGCACCTGTT ATGTTGGACA AACAACTGTC 
AAAGAGATTT ATCTTCACAA CAGAGGTGGC TCCTGTAGCT ACTGGACCAC ACTCACAGAT 
GGCAATGAAG TGTTCAGGGT AAGTCCGGAT TCTGGTTTAC TGAAACCTCT TGGAGATCCA 
GCGTCCTGTA GACAGCTGCT GCAGATCAGC TTCACTCCGA GAGAACAGAA AGAGTTCCAG 
ACAATCTTCA CAGTGCAGGG GCTTCTTGGA GAGACACCAC TTGTTCTAAA GGTCAGCGGA 
AGAGGATCAT TTGATGAGAG TTTTGTATCT CCAATAACTG ACAGCTCACA AAACAACACT 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209444.1
CDS	197..5302
Translation	MADETEPKALTLKSSEPSINRHTPASKTTQDISHLLASIFNDLYSTEVIGKDTVSNLSKSRRRGNSYHEKYVEELQQVHSEYNRRIQNADMLETHIIQARLQAAAKEEHDRNKILEEVGEAYQQLGLPPVKSAFKWCVDSGLLRSNSLICPLDYTTVHNPVVKSPKARSSPGFAKPTFSYNMHISTELQDDGYTPLPPHAHSAHSLLEYSEETITLSSSPESSSINDTELGLVAKASWMEKTSAKSHAEDRASLKKHKDRHKFLHNPRFLLPNTQHGGKSLIIPGKKKLENVGKSRKNNGRESLEGHAPIFTANPPVIFFSDYRVGQVYETSVELKNMTASSRHIRMIPPTSPHFSVGLGRFPGEGGIVAPGMSCQYMVRFAPDSLADYEDALVVETQMLYPLIIPIEACRSPPILTLPAVMDCGFCLVGGVKFMEVLCRNEGLSAGTFCVMPKKQWPASSLRSAVKSTFTEQPPFAISPSIFGLHPGQATVIEVVFFPTTPELSTQDFTIVCDNCQVKDITLQGTGQLITVELLSVSGGEDQPEFAELCDITADHYVRFNTTNPHSTLQKTLVVKNNTHLELPYHWQIMKPSLQCLLPGETPDPSSIQHLLDTDSAFSISPVIGILNPAQEHEFLLTYAPKDIKDYHSVCHLVIMDVPSPPKVCDDGTCPDTALHANNVTILEVEVKGSTEPYKILLEPYAVLIPGETYIHNTICKRFKMWNHSKSVIHFEWERVIDSHVIEVEPSSGVIEMNECFDMELYLTGRSPGHFTSTLQCHILHHPKTIGLAIEATFKGPHCSLNVPSLDFGLLQMGNERILTIEVTNNSLLDAHWSLEVLSQTQKLIDGQVHINPNKGVLAALESCSVDVAFRAMHCQVFESVLQLSVLNGTGCHVSIHAVVQSPQVCLLNCELLFADLYVGVSQTGSVTLFNQTLLPAHFTWGRLQGSQAHLCSAVFSPSAGSLGPNAKTEVSVSFTAHTVEDLTEVNGVCEVKGMENPLVLGIFSKAKPLRVSYSLLCNGINSGDGDHQPILLDFTEHEPAVIGKSVKRQLLITNNTAITAPFTVDTEYFTGDCPSQSEGNSERSSAPFHSIQAKKIQQKAYDEFVSRLLSHGRGATFYVEPKSGMLGPFESQTINITTFTNMWGDYQDNLICKVGDLNPTHIHMKMSVRGCPVYFQMIGPQPDNQNQGPVIRFGSHVSGGDTVSRSLRLINTSPYDIRMDWVTYNLEAGDGKLIDLLLACGEPFPLKDADGNEVVGGLDSSVMFPSTWDDSHTPSREGTSSTFMTQSDDFVENGEECDGEDGSGASESLAPVKKLFSVFLKPHEGTLSDYPYCITPQQTVVPAGGSSTIHLSFTPLILSCSTNQRCLGYALGFMSLDSKLALVTPGKVERVRGYELQPLRLDMQANVKPAILSVQMAEDTEVLEFSAAASDTLDGASQTQESRITRTFRLKNDTDMALSFKLSTNPPFSVLLPRQSLKLISSSPAYRHTQGLSGPGDEMRLFLLQPKNIIQVKVAFHNSASLLACVNGACEESDSQLSASLLCSDDGGKTLQFQENLTIQYSNNSIQTVSLYARLALPTLHLSSATVDFGTCYVGQTTVKEIYLHNRGGSCSYWTTLTDGNEVFRVSPDSGLLKPLGDPASCRQLLQISFTPREQKEFQTIFTVQGLLGETPLVLKVSGRGSFDESFVSPITDSSQNNTE

Target ORF information:

RefSeq Version	XM_017353955.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353955.2

ORF Insert Sequence:

ATGGCTGATG AAACTGAACC AAAAGCACTA ACGTTAAAGA GTTCAGAACC GTCAATAAAC 
CGGCACACAC CAGCTTCAAA GACAACTCAG GACATTTCTC ATCTGCTGGC CAGCATATTC 
AATGATCTCT ACAGCACAGA GGTGATTGGG AAGGACACCG TTTCCAATCT CAGTAAATCA 
CGAAGAAGAG GCAACAGCTA CCATGAAAAA TATGTTGAGG AGCTACAACA GGTGCACAGT 
GAATACAACA GACGTATTCA GAATGCTGAT ATGCTAGAGA CACACATCAT ACAGGCCCGT 
CTTCAGGCTG CTGCAAAAGA AGAGCATGAT CGCAACAAAA TCCTGGAAGA AGTCGGTGAG 
GCTTACCAAC AGCTGGGACT TCCCCCAGTA AAATCAGCTT TCAAGTGGTG TGTGGACAGT 
GGACTTTTGA GGAGCAACAG CTTGATTTGC CCACTGGACT ACACAACAGT GCACAACCCA 
GTTGTTAAAA GCCCAAAAGC CAGGTCAAGT CCAGGCTTTG CCAAGCCTAC TTTCTCTTAT 
AATATGCACA TCTCCACTGA ACTGCAGGAT GATGGTTACA CTCCGCTTCC TCCACACGCT 
CATTCAGCAC ACAGCCTGCT TGAATATTCA GAGGAGACAA TCACACTCTC TTCCTCTCCA 
GAGTCCAGCT CCATCAATGA CACAGAGCTA GGTTTGGTGG CGAAGGCTTC TTGGATGGAG 
AAGACCAGTG CTAAGAGTCA TGCAGAAGAC AGGGCTTCCC TGAAAAAGCA CAAAGACCGG 
CATAAGTTTC TGCACAACCC TCGCTTCTTG CTGCCAAACA CTCAGCATGG AGGAAAGTCT 
CTCATCATCC CAGGGAAAAA GAAGCTGGAG AATGTGGGAA AGAGCAGGAA AAACAACGGC 
AGAGAGAGTC TTGAGGGTCA TGCGCCTATT TTCACTGCAA ATCCACCAGT GATCTTCTTC 
AGTGACTACA GAGTAGGACA AGTCTATGAG ACCTCAGTGG AGCTCAAAAA CATGACTGCG 
AGCAGCCGTC ACATTCGAAT GATTCCTCCA ACCTCCCCAC ACTTCTCTGT GGGTTTGGGC 
CGGTTCCCTG GTGAAGGAGG CATCGTGGCT CCAGGAATGA GCTGTCAGTA TATGGTGCGT 
TTTGCTCCAG ATTCTCTAGC AGACTATGAA GACGCTCTGG TTGTGGAGAC GCAGATGCTC 
TATCCTCTCA TTATACCAAT AGAAGCCTGC AGATCTCCTC CGATACTCAC CTTGCCGGCT 
GTCATGGACT GTGGTTTCTG CCTGGTGGGA GGAGTGAAGT TTATGGAGGT TTTATGTCGG 
AATGAAGGAC TGAGTGCTGG AACGTTTTGT GTAATGCCTA AGAAACAGTG GCCTGCTTCA 
AGCCTCAGGT CTGCAGTGAA GTCGACTTTC ACAGAACAGC CTCCCTTTGC TATCAGTCCC 
TCTATTTTCG GCCTTCACCC TGGACAGGCT ACTGTCATAG AGGTTGTGTT TTTCCCCACA 
ACTCCTGAGC TCTCTACTCA AGACTTCACC ATTGTCTGTG ACAACTGCCA AGTGAAGGAC 
ATTACGCTCC AAGGTACTGG TCAACTGATC ACTGTGGAGC TGCTTTCAGT ATCAGGTGGA 
GAGGATCAGC CTGAATTTGC AGAATTGTGT GACATCACAG CTGACCATTA TGTCAGATTT 
AACACAACCA ATCCACACTC TACCCTGCAG AAGACACTGG TTGTCAAAAA CAACACACAT 
TTGGAGCTTC CATATCACTG GCAGATCATG AAGCCCAGTC TTCAATGCTT GTTGCCCGGA 
GAGACTCCTG ACCCTTCCAG CATTCAGCAT CTTCTTGATA CAGACAGTGC ATTCAGCATC 
AGTCCTGTTA TAGGAATCCT GAACCCTGCA CAGGAACACG AGTTCCTGCT CACCTATGCT 
CCCAAAGATA TAAAAGATTA CCACAGTGTC TGCCACTTGG TGATTATGGA TGTTCCCAGT 
CCACCTAAAG TCTGTGACGA TGGGACATGT CCTGATACTG CACTCCATGC AAACAATGTG 
ACCATTTTAG AGGTTGAAGT GAAAGGTTCT ACTGAGCCAT ACAAGATCCT TCTTGAGCCG 
TATGCTGTTT TAATACCAGG AGAGACCTAT ATACACAACA CAATTTGCAA GAGATTCAAG 
ATGTGGAATC ACAGCAAATC AGTCATTCAC TTTGAATGGG AACGTGTTAT TGACAGTCAT 
GTAATTGAAG TGGAGCCCTC ATCTGGAGTA ATAGAAATGA ATGAGTGTTT TGACATGGAG 
TTGTATCTGA CTGGAAGGAG TCCGGGTCAT TTTACCTCTA CCCTGCAGTG TCACATTCTG 
CACCATCCTA AAACTATAGG ACTTGCCATT GAGGCCACAT TTAAGGGTCC ACATTGCTCA 
TTGAATGTAC CCAGTCTGGA TTTTGGTTTG CTGCAAATGG GAAATGAGAG AATTTTGACT 
ATTGAAGTCA CCAACAACAG CCTGCTAGAT GCCCACTGGA GCCTGGAGGT GCTGTCCCAA 
ACCCAGAAGC TCATCGATGG ACAGGTGCAT ATAAATCCAA ATAAAGGTGT TCTGGCTGCA 
CTAGAGAGCT GCAGTGTGGA TGTGGCCTTC AGAGCCATGC ATTGCCAAGT ATTTGAGTCA 
GTTTTACAAC TTTCTGTGCT GAACGGCACC GGTTGTCACG TCTCCATCCA TGCCGTTGTT 
CAGTCTCCTC AAGTGTGCTT GCTGAACTGT GAGTTGCTGT TTGCTGATCT GTATGTTGGG 
GTTTCACAGA CGGGCAGTGT CACTCTGTTT AACCAAACAC TACTGCCAGC TCACTTCACC 
TGGGGCAGGC TTCAAGGTTC ACAAGCTCAT CTCTGCTCCG CAGTCTTCAG TCCCTCTGCA 
GGATCTCTGG GGCCCAATGC AAAAACTGAA GTCTCTGTGT CATTCACAGC TCACACTGTT 
GAGGATTTGA CTGAAGTGAA TGGAGTTTGT GAGGTAAAAG GAATGGAGAA TCCACTTGTG 
CTGGGAATTT TTTCTAAAGC CAAACCTCTC AGAGTTTCCT ACTCACTGCT TTGTAATGGT 
ATTAATTCAG GTGATGGCGA TCACCAACCA ATCCTGCTGG ACTTCACTGA GCATGAGCCA 
GCCGTCATTG GGAAATCTGT CAAACGGCAG TTACTGATAA CTAACAACAC TGCTATTACT 
GCTCCATTCA CTGTCGACAC TGAGTACTTC ACTGGAGACT GTCCTTCACA ATCAGAAGGA 
AACTCTGAAA GAAGCAGTGC ACCTTTCCAC TCCATCCAGG CCAAAAAAAT ACAGCAAAAG 
GCATATGATG AGTTTGTAAG CCGTCTGCTT TCCCATGGAA GAGGTGCAAC ATTTTATGTA 
GAACCAAAAA GTGGGATGCT TGGCCCATTC GAGAGTCAAA CCATCAACAT CACTACTTTC 
ACAAATATGT GGGGGGATTA TCAGGACAAC CTCATATGCA AAGTTGGAGA TCTAAATCCA 
ACTCACATTC ATATGAAGAT GTCTGTGAGG GGCTGTCCAG TTTACTTTCA GATGATTGGA 
CCACAGCCTG ACAATCAGAA TCAGGGTCCA GTTATTAGAT TTGGAAGCCA TGTTTCTGGA 
GGAGACACTG TGTCACGCTC TTTACGACTC ATTAACACCA GTCCATATGA TATCCGTATG 
GACTGGGTGA CCTACAACCT GGAGGCTGGA GACGGCAAGC TGATAGATCT GCTGCTTGCA 
TGTGGTGAAC CTTTCCCTCT GAAGGATGCA GATGGCAATG AGGTTGTTGG AGGACTGGAC 
TCATCTGTCA TGTTCCCGTC CACATGGGAT GACAGTCACA CCCCCAGCAG AGAAGGAACA 
AGCTCAACTT TTATGACCCA ATCTGATGAT TTTGTAGAGA ATGGTGAAGA ATGTGATGGA 
GAAGATGGCA GTGGGGCTTC TGAGTCTCTA GCTCCAGTGA AGAAGCTGTT TTCTGTGTTT 
TTGAAGCCTC ATGAAGGGAC TCTTTCTGAC TACCCATACT GCATCACTCC ACAGCAGACT 
GTAGTTCCAG CAGGGGGCAG TAGCACCATT CATTTGTCTT TCACTCCCCT GATTCTGTCA 
TGTTCAACCA ATCAAAGATG TTTGGGTTAC GCTCTTGGTT TCATGAGCTT AGACTCTAAG 
CTTGCGTTAG TGACTCCAGG CAAGGTGGAG AGAGTCCGGG GCTATGAACT TCAACCTTTG 
AGGCTGGACA TGCAGGCTAA TGTAAAACCT GCCATTTTGT CAGTGCAGAT GGCAGAGGAT 
ACAGAAGTGC TGGAATTCAG CGCAGCTGCC AGCGACACAC TAGACGGAGC ATCACAAACA 
CAGGAATCCA GGATAACTCG CACATTCCGG CTAAAGAACG ACACAGACAT GGCCTTGAGT 
TTCAAACTGA GCACAAATCC ACCATTTTCA GTGCTGCTGC CCCGACAGAG CCTTAAACTG 
ATCAGCAGCT CGCCTGCTTA TAGACACACA CAAGGTCTGT CCGGACCAGG AGATGAGATG 
AGATTATTTC TGTTACAGCC CAAAAACATC ATTCAGGTTA AGGTGGCTTT CCACAATTCC 
GCTTCACTCC TGGCCTGTGT AAATGGAGCA TGTGAGGAGT CGGACAGTCA GCTCTCTGCT 
TCCCTTCTTT GCAGTGATGA TGGAGGAAAA ACTCTACAGT TTCAGGAGAA CTTGACCATT 
CAGTACAGCA ATAACAGCAT ACAGACAGTC TCTCTTTACG CTCGTCTGGC ATTACCTACA 
CTACACCTGT CCAGTGCCAC TGTGGATTTT GGCACCTGTT ATGTTGGACA AACAACTGTC 
AAAGAGATTT ATCTTCACAA CAGAGGTGGC TCCTGTAGCT ACTGGACCAC ACTCACAGAT 
GGCAATGAAG TGTTCAGGGT AAGTCCGGAT TCTGGTTTAC TGAAACCTCT TGGAGATCCA 
GCGTCCTGTA GACAGCTGCT GCAGATCAGC TTCACTCCGA GAGAACAGAA AGAGTTCCAG 
ACAATCTTCA CAGTGCAGGG GCTTCTTGGA GAGACACCAC TTGTTCTAAA GGTCAGCGGA 
AGAGGATCAT TTGATGAGAG TTTTGTATCT CCAATAACTG ACAGCTCACA AAACAACACT 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlec1 ( XM_017353956.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209445.1 Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa57707
Clone ID Related Accession (Same CDS sequence)	XM_017353956.1 , XM_017353956.2
Accession Version	XM_017353956.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	deleted in lung and esophageal cancer protein 1 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATG AAACTGAACC AAAAGCACTA ACGTTAAAGA GTTCAGAACC GTCAATAAAC 
CGGCACACAC CAGCTTCAAA GACAACTCAG GACATTTCTC ATCTGCTGGC CAGCATATTC 
AATGATCTCT ACAGCACAGA GGTGATTGGG AAGGACACCG TTTCCAATCT CAGTAAATCA 
CGAAGAAGAG GCAACAGCTA CCATGAAAAA TATGTTGAGG AGCTACAACA GGTGCACAGT 
GAATACAACA GACGTATTCA GAATGCTGAT ATGCTAGAGA CACACATCAT ACAGGCCCGT 
CTTCAGGCTG CTGCAAAAGA AGAGCATGAT CGCAACAAAA TCCTGGAAGA AGTCGGTGAG 
GCTTACCAAC AGCTGGGACT TCCCCCAGTA AAATCAGCTT TCAAGTGGTG TGTGGACAGT 
GGACTTTTGA GGAGCAACAG CTTGATTTGC CCACTGGACT ACACAACAGT GCACAACCCA 
GTTGTTAAAA GCCCAAAAGC CAGGTCAAGT CCAGGCTTTG CCAAGCCTAC TTTCTCTTAT 
AATATGCACA TCTCCACTGA ACTGCAGGAT GATGGTTACA CTCCGCTTCC TCCACACGCT 
CATTCAGCAC ACAGCCTGCT TGAATATTCA GAGGAGACAA TCACACTCTC TTCCTCTCCA 
GAGTCCAGCT CCATCAATGA CACAGAGCTA GGTTTGGTGG CGAAGGCTTC TTGGATGGAG 
AAGACCAGTG CTAAGAGTCA TGCAGAAGAC AGGGCTTCCC TGAAAAAGCA CAAAGACCGG 
CATAAGTTTC TGCACAACCC TCGCTTCTTG CTGCCAAACA CTCAGCATGG AGGAAAGTCT 
CTCATCATCC CAGGGAAAAA GAAGCTGGAG AATGTGGGAA AGAGCAGGAA AAACAACGGC 
AGAGAGAGAA GTCTTGAGGG TCATGCGCCT ATTTTCACTG CAAATCCACC AGTGATCTTC 
TTCAGTGACT ACAGAGTAGG ACAAGTCTAT GAGACCTCAG TGGAGCTCAA AAACATGACT 
GCGAGCAGCC GTCACATTCG AATGATTCCT CCAACCTCCC CACACTTCTC TGTGGGTTTG 
GGCCGGTTCC CTGGTGAAGG AGGCATCGTG GCTCCAGGAA TGAGCTGTCA GTATATGGTG 
CGTTTTGCTC CAGATTCTCT AGCAGACTAT GAAGACGCTC TGGTTGTGGA GACGCAGATG 
CTCTATCCTC TCATTATACC AATAGAAGCC TGCAGATCTC CTCCGATACT CACCTTGCCG 
GCTGTCATGG ACTGTGGTTT CTGCCTGGTG GGAGGAGTGA AGTTTATGGA GGTTTTATGT 
CGGAATGAAG GACTGAGTGC TGGAACGTTT TGTGTAATGC CTAAGAAACA GTGGCCTGCT 
TCAAGCCTCA GGTCTGCAGT GAAGTCGACT TTCACAGAAC AGCCTCCCTT TGCTATCAGT 
CCCTCTATTT TCGGCCTTCA CCCTGGACAG GCTACTGTCA TAGAGGTTGT GTTTTTCCCC 
ACAACTCCTG AGCTCTCTAC TCAAGACTTC ACCATTGTCT GTGACAACTG CCAAGTGAAG 
GACATTACGC TCCAAGGTAC TGGTCAACTG ATCACTGTGG AGCTGCTTTC AGTATCAGGT 
GGAGAGGATC AGCCTGAATT TGCAGAATTG TGTGACATCA CAGCTGACCA TTATGTCAGA 
TTTAACACAA CCAATCCACA CTCTACCCTG CAGAAGACAC TGGTTGTCAA AAACAACACA 
CATTTGGAGC TTCCATATCA CTGGCAGATC ATGAAGCCCA GTCTTCAATG CTTGTTGCCC 
GGAGAGACTC CTGACCCTTC CAGCATTCAG CATCTTCTTG ATACAGACAG TGCATTCAGC 
ATCAGTCCTG TTATAGGAAT CCTGAACCCT GCACAGGAAC ACGAGTTCCT GCTCACCTAT 
GCTCCCAAAG ATATAAAAGA TTACCACAGT GTCTGCCACT TGGTGATTAT GGATGTTCCC 
AGTCCACCTA AAGTCTGTGA CGATGGGACA TGTCCTGATA CTGCACTCCA TGCAAACAAT 
GTGACCATTT TAGAGGTTGA AGTGAAAGGT TCTACTGAGC CATACAAGAT CCTTCTTGAG 
CCGTATGCTG TTTTAATACC AGGAGAGACC TATATACACA ACACAATTTG CAAGAGATTC 
AAGATGTGGA ATCACAGCAA ATCAGTCATT CACTTTGAAT GGGAACGTGT TATTGACAGT 
CATGTAATTG AAGTGGAGCC CTCATCTGGA GTAATAGAAA TGAATGAGTG TTTTGACATG 
GAGTTGTATC TGACTGGAAG GAGTCCGGGT CATTTTACCT CTACCCTGCA GTGTCACATT 
CTGCACCATC CTAAAACTAT AGGACTTGCC ATTGAGGCCA CATTTAAGGG TCCACATTGC 
TCATTGAATG TACCCAGTCT GGATTTTGGT TTGCTGCAAA TGGGAAATGA GAGAATTTTG 
ACTATTGAAG TCACCAACAA CAGCCTGCTA GATGCCCACT GGAGCCTGGA GGTGCTGTCC 
CAAACCCAGA AGCTCATCGA TGGACAGGTG CATATAAATC CAAATAAAGG TGTTCTGGCT 
GCACTAGAGA GCTGCAGTGT GGATGTGGCC TTCAGAGCCA TGCATTGCCA AGTATTTGAG 
TCAGTTTTAC AACTTTCTGT GCTGAACGGC ACCGGTTGTC ACGTCTCCAT CCATGCCGTT 
GTTCAGTCTC CTCAAGTGTG CTTGCTGAAC TGTGAGTTGC TGTTTGCTGA TCTGTATGTT 
GGGGTTTCAC AGACGGGCAG TGTCACTCTG TTTAACCAAA CACTACTGCC AGCTCACTTC 
ACCTGGGGCA GGCTTCAAGG TTCACAAGCT CATCTCTGCT CCGCAGTCTT CAGTCCCTCT 
GCAGGATCTC TGGGGCCCAA TGCAAAAACT GAAGTCTCTG TGTCATTCAC AGCTCACACT 
GTTGAGGATT TGACTGAAGT GAATGGAGTT TGTGAGGTAA AAGGAATGGA GAATCCACTT 
GTGCTGGGAA TTTTTTCTAA AGCCAAACCT CTCAGAGTTT CCTACTCACT GCTTTGTAAT 
GGTATTAATT CAGGTGATGG CGATCACCAA CCAATCCTGC TGGACTTCAC TGAGCATGAG 
CCAGCCGTCA TTGGGAAATC TGTCAAACGG CAGTTACTGA TAACTAACAA CACTGCTATT 
ACTGCTCCAT TCACTGTCGA CACTGAGTAC TTCACTGGAG ACTGTCCTTC ACAATCAGAA 
GGAAACTCTG AAAGCAGTGC ACCTTTCCAC TCCATCCAGG CCAAAAAAAT ACAGCAAAAG 
GCATATGATG AGTTTGTAAG CCGTCTGCTT TCCCATGGAA GAGGTGCAAC ATTTTATGTA 
GAACCAAAAA GTGGGATGCT TGGCCCATTC GAGAGTCAAA CCATCAACAT CACTACTTTC 
ACAAATATGT GGGGGGATTA TCAGGACAAC CTCATATGCA AAGTTGGAGA TCTAAATCCA 
ACTCACATTC ATATGAAGAT GTCTGTGAGG GGCTGTCCAG TTTACTTTCA GATGATTGGA 
CCACAGCCTG ACAATCAGAA TCAGGGTCCA GTTATTAGAT TTGGAAGCCA TGTTTCTGGA 
GGAGACACTG TGTCACGCTC TTTACGACTC ATTAACACCA GTCCATATGA TATCCGTATG 
GACTGGGTGA CCTACAACCT GGAGGCTGGA GACGGCAAGC TGATAGATCT GCTGCTTGCA 
TGTGGTGAAC CTTTCCCTCT GAAGGATGCA GATGGCAATG AGGTTGTTGG AGGACTGGAC 
TCATCTGTCA TGTTCCCGTC CACATGGGAT GACAGTCACA CCCCCAGCAG AGAAGGAACA 
AGCTCAACTT TTATGACCCA ATCTGATGAT TTTGTAGAGA ATGGTGAAGA ATGTGATGGA 
GAAGATGGCA GTGGGGCTTC TGAGTCTCTA GCTCCAGTGA AGAAGCTGTT TTCTGTGTTT 
TTGAAGCCTC ATGAAGGGAC TCTTTCTGAC TACCCATACT GCATCACTCC ACAGCAGACT 
GTAGTTCCAG CAGGGGGCAG TAGCACCATT CATTTGTCTT TCACTCCCCT GATTCTGTCA 
TGTTCAACCA ATCAAAGATG TTTGGGTTAC GCTCTTGGTT TCATGAGCTT AGACTCTAAG 
CTTGCGTTAG TGACTCCAGG CAAGGTGGAG AGAGTCCGGG GCTATGAACT TCAACCTTTG 
AGGCTGGACA TGCAGGCTAA TGTAAAACCT GCCATTTTGT CAGTGCAGAT GGCAGAGGAT 
ACAGAAGTGC TGGAATTCAG CGCAGCTGCC AGCGACACAC TAGACGGAGC ATCACAAACA 
CAGGAATCCA GGATAACTCG CACATTCCGG CTAAAGAACG ACACAGACAT GGCCTTGAGT 
TTCAAACTGA GCACAAATCC ACCATTTTCA GTGCTGCTGC CCCGACAGAG CCTTAAACTG 
ATCAGCAGCT CGCCTGCTTA TAGACACACA CAAGGTCTGT CCGGACCAGG AGATGAGATG 
AGATTATTTC TGTTACAGCC CAAAAACATC ATTCAGGTTA AGGTGGCTTT CCACAATTCC 
GCTTCACTCC TGGCCTGTGT AAATGGAGCA TGTGAGGAGT CGGACAGTCA GCTCTCTGCT 
TCCCTTCTTT GCAGTGATGA TGGAGGAAAA ACTCTACAGT TTCAGGAGAA CTTGACCATT 
CAGTACAGCA ATAACAGCAT ACAGACAGTC TCTCTTTACG CTCGTCTGGC ATTACCTACA 
CTACACCTGT CCAGTGCCAC TGTGGATTTT GGCACCTGTT ATGTTGGACA AACAACTGTC 
AAAGAGATTT ATCTTCACAA CAGAGGTGGC TCCTGTAGCT ACTGGACCAC ACTCACAGAT 
GGCAATGAAG TGTTCAGGGT AAGTCCGGAT TCTGGTTTAC TGAAACCTCT TGGAGATCCA 
GCGTCCTGTA GACAGCTGCT GCAGATCAGC TTCACTCCGA GAGAACAGAA AGAGTTCCAG 
ACAATCTTCA CAGTGCAGGG GCTTCTTGGA GAGACACCAC TTGTTCTAAA GGTCAGCGGA 
AGAGGATCAT TTGATGAGAG TTTTGTATCT CCAATAACTG ACAGCTCACA AAACAACACT 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209445.1
CDS	180..5285
Translation	MADETEPKALTLKSSEPSINRHTPASKTTQDISHLLASIFNDLYSTEVIGKDTVSNLSKSRRRGNSYHEKYVEELQQVHSEYNRRIQNADMLETHIIQARLQAAAKEEHDRNKILEEVGEAYQQLGLPPVKSAFKWCVDSGLLRSNSLICPLDYTTVHNPVVKSPKARSSPGFAKPTFSYNMHISTELQDDGYTPLPPHAHSAHSLLEYSEETITLSSSPESSSINDTELGLVAKASWMEKTSAKSHAEDRASLKKHKDRHKFLHNPRFLLPNTQHGGKSLIIPGKKKLENVGKSRKNNGRERSLEGHAPIFTANPPVIFFSDYRVGQVYETSVELKNMTASSRHIRMIPPTSPHFSVGLGRFPGEGGIVAPGMSCQYMVRFAPDSLADYEDALVVETQMLYPLIIPIEACRSPPILTLPAVMDCGFCLVGGVKFMEVLCRNEGLSAGTFCVMPKKQWPASSLRSAVKSTFTEQPPFAISPSIFGLHPGQATVIEVVFFPTTPELSTQDFTIVCDNCQVKDITLQGTGQLITVELLSVSGGEDQPEFAELCDITADHYVRFNTTNPHSTLQKTLVVKNNTHLELPYHWQIMKPSLQCLLPGETPDPSSIQHLLDTDSAFSISPVIGILNPAQEHEFLLTYAPKDIKDYHSVCHLVIMDVPSPPKVCDDGTCPDTALHANNVTILEVEVKGSTEPYKILLEPYAVLIPGETYIHNTICKRFKMWNHSKSVIHFEWERVIDSHVIEVEPSSGVIEMNECFDMELYLTGRSPGHFTSTLQCHILHHPKTIGLAIEATFKGPHCSLNVPSLDFGLLQMGNERILTIEVTNNSLLDAHWSLEVLSQTQKLIDGQVHINPNKGVLAALESCSVDVAFRAMHCQVFESVLQLSVLNGTGCHVSIHAVVQSPQVCLLNCELLFADLYVGVSQTGSVTLFNQTLLPAHFTWGRLQGSQAHLCSAVFSPSAGSLGPNAKTEVSVSFTAHTVEDLTEVNGVCEVKGMENPLVLGIFSKAKPLRVSYSLLCNGINSGDGDHQPILLDFTEHEPAVIGKSVKRQLLITNNTAITAPFTVDTEYFTGDCPSQSEGNSESSAPFHSIQAKKIQQKAYDEFVSRLLSHGRGATFYVEPKSGMLGPFESQTINITTFTNMWGDYQDNLICKVGDLNPTHIHMKMSVRGCPVYFQMIGPQPDNQNQGPVIRFGSHVSGGDTVSRSLRLINTSPYDIRMDWVTYNLEAGDGKLIDLLLACGEPFPLKDADGNEVVGGLDSSVMFPSTWDDSHTPSREGTSSTFMTQSDDFVENGEECDGEDGSGASESLAPVKKLFSVFLKPHEGTLSDYPYCITPQQTVVPAGGSSTIHLSFTPLILSCSTNQRCLGYALGFMSLDSKLALVTPGKVERVRGYELQPLRLDMQANVKPAILSVQMAEDTEVLEFSAAASDTLDGASQTQESRITRTFRLKNDTDMALSFKLSTNPPFSVLLPRQSLKLISSSPAYRHTQGLSGPGDEMRLFLLQPKNIIQVKVAFHNSASLLACVNGACEESDSQLSASLLCSDDGGKTLQFQENLTIQYSNNSIQTVSLYARLALPTLHLSSATVDFGTCYVGQTTVKEIYLHNRGGSCSYWTTLTDGNEVFRVSPDSGLLKPLGDPASCRQLLQISFTPREQKEFQTIFTVQGLLGETPLVLKVSGRGSFDESFVSPITDSSQNNTE

Target ORF information:

RefSeq Version	XM_017353956.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353956.1

ORF Insert Sequence:

ATGGCTGATG AAACTGAACC AAAAGCACTA ACGTTAAAGA GTTCAGAACC GTCAATAAAC 
CGGCACACAC CAGCTTCAAA GACAACTCAG GACATTTCTC ATCTGCTGGC CAGCATATTC 
AATGATCTCT ACAGCACAGA GGTGATTGGG AAGGACACCG TTTCCAATCT CAGTAAATCA 
CGAAGAAGAG GCAACAGCTA CCATGAAAAA TATGTTGAGG AGCTACAACA GGTGCACAGT 
GAATACAACA GACGTATTCA GAATGCTGAT ATGCTAGAGA CACACATCAT ACAGGCCCGT 
CTTCAGGCTG CTGCAAAAGA AGAGCATGAT CGCAACAAAA TCCTGGAAGA AGTCGGTGAG 
GCTTACCAAC AGCTGGGACT TCCCCCAGTA AAATCAGCTT TCAAGTGGTG TGTGGACAGT 
GGACTTTTGA GGAGCAACAG CTTGATTTGC CCACTGGACT ACACAACAGT GCACAACCCA 
GTTGTTAAAA GCCCAAAAGC CAGGTCAAGT CCAGGCTTTG CCAAGCCTAC TTTCTCTTAT 
AATATGCACA TCTCCACTGA ACTGCAGGAT GATGGTTACA CTCCGCTTCC TCCACACGCT 
CATTCAGCAC ACAGCCTGCT TGAATATTCA GAGGAGACAA TCACACTCTC TTCCTCTCCA 
GAGTCCAGCT CCATCAATGA CACAGAGCTA GGTTTGGTGG CGAAGGCTTC TTGGATGGAG 
AAGACCAGTG CTAAGAGTCA TGCAGAAGAC AGGGCTTCCC TGAAAAAGCA CAAAGACCGG 
CATAAGTTTC TGCACAACCC TCGCTTCTTG CTGCCAAACA CTCAGCATGG AGGAAAGTCT 
CTCATCATCC CAGGGAAAAA GAAGCTGGAG AATGTGGGAA AGAGCAGGAA AAACAACGGC 
AGAGAGAGAA GTCTTGAGGG TCATGCGCCT ATTTTCACTG CAAATCCACC AGTGATCTTC 
TTCAGTGACT ACAGAGTAGG ACAAGTCTAT GAGACCTCAG TGGAGCTCAA AAACATGACT 
GCGAGCAGCC GTCACATTCG AATGATTCCT CCAACCTCCC CACACTTCTC TGTGGGTTTG 
GGCCGGTTCC CTGGTGAAGG AGGCATCGTG GCTCCAGGAA TGAGCTGTCA GTATATGGTG 
CGTTTTGCTC CAGATTCTCT AGCAGACTAT GAAGACGCTC TGGTTGTGGA GACGCAGATG 
CTCTATCCTC TCATTATACC AATAGAAGCC TGCAGATCTC CTCCGATACT CACCTTGCCG 
GCTGTCATGG ACTGTGGTTT CTGCCTGGTG GGAGGAGTGA AGTTTATGGA GGTTTTATGT 
CGGAATGAAG GACTGAGTGC TGGAACGTTT TGTGTAATGC CTAAGAAACA GTGGCCTGCT 
TCAAGCCTCA GGTCTGCAGT GAAGTCGACT TTCACAGAAC AGCCTCCCTT TGCTATCAGT 
CCCTCTATTT TCGGCCTTCA CCCTGGACAG GCTACTGTCA TAGAGGTTGT GTTTTTCCCC 
ACAACTCCTG AGCTCTCTAC TCAAGACTTC ACCATTGTCT GTGACAACTG CCAAGTGAAG 
GACATTACGC TCCAAGGTAC TGGTCAACTG ATCACTGTGG AGCTGCTTTC AGTATCAGGT 
GGAGAGGATC AGCCTGAATT TGCAGAATTG TGTGACATCA CAGCTGACCA TTATGTCAGA 
TTTAACACAA CCAATCCACA CTCTACCCTG CAGAAGACAC TGGTTGTCAA AAACAACACA 
CATTTGGAGC TTCCATATCA CTGGCAGATC ATGAAGCCCA GTCTTCAATG CTTGTTGCCC 
GGAGAGACTC CTGACCCTTC CAGCATTCAG CATCTTCTTG ATACAGACAG TGCATTCAGC 
ATCAGTCCTG TTATAGGAAT CCTGAACCCT GCACAGGAAC ACGAGTTCCT GCTCACCTAT 
GCTCCCAAAG ATATAAAAGA TTACCACAGT GTCTGCCACT TGGTGATTAT GGATGTTCCC 
AGTCCACCTA AAGTCTGTGA CGATGGGACA TGTCCTGATA CTGCACTCCA TGCAAACAAT 
GTGACCATTT TAGAGGTTGA AGTGAAAGGT TCTACTGAGC CATACAAGAT CCTTCTTGAG 
CCGTATGCTG TTTTAATACC AGGAGAGACC TATATACACA ACACAATTTG CAAGAGATTC 
AAGATGTGGA ATCACAGCAA ATCAGTCATT CACTTTGAAT GGGAACGTGT TATTGACAGT 
CATGTAATTG AAGTGGAGCC CTCATCTGGA GTAATAGAAA TGAATGAGTG TTTTGACATG 
GAGTTGTATC TGACTGGAAG GAGTCCGGGT CATTTTACCT CTACCCTGCA GTGTCACATT 
CTGCACCATC CTAAAACTAT AGGACTTGCC ATTGAGGCCA CATTTAAGGG TCCACATTGC 
TCATTGAATG TACCCAGTCT GGATTTTGGT TTGCTGCAAA TGGGAAATGA GAGAATTTTG 
ACTATTGAAG TCACCAACAA CAGCCTGCTA GATGCCCACT GGAGCCTGGA GGTGCTGTCC 
CAAACCCAGA AGCTCATCGA TGGACAGGTG CATATAAATC CAAATAAAGG TGTTCTGGCT 
GCACTAGAGA GCTGCAGTGT GGATGTGGCC TTCAGAGCCA TGCATTGCCA AGTATTTGAG 
TCAGTTTTAC AACTTTCTGT GCTGAACGGC ACCGGTTGTC ACGTCTCCAT CCATGCCGTT 
GTTCAGTCTC CTCAAGTGTG CTTGCTGAAC TGTGAGTTGC TGTTTGCTGA TCTGTATGTT 
GGGGTTTCAC AGACGGGCAG TGTCACTCTG TTTAACCAAA CACTACTGCC AGCTCACTTC 
ACCTGGGGCA GGCTTCAAGG TTCACAAGCT CATCTCTGCT CCGCAGTCTT CAGTCCCTCT 
GCAGGATCTC TGGGGCCCAA TGCAAAAACT GAAGTCTCTG TGTCATTCAC AGCTCACACT 
GTTGAGGATT TGACTGAAGT GAATGGAGTT TGTGAGGTAA AAGGAATGGA GAATCCACTT 
GTGCTGGGAA TTTTTTCTAA AGCCAAACCT CTCAGAGTTT CCTACTCACT GCTTTGTAAT 
GGTATTAATT CAGGTGATGG CGATCACCAA CCAATCCTGC TGGACTTCAC TGAGCATGAG 
CCAGCCGTCA TTGGGAAATC TGTCAAACGG CAGTTACTGA TAACTAACAA CACTGCTATT 
ACTGCTCCAT TCACTGTCGA CACTGAGTAC TTCACTGGAG ACTGTCCTTC ACAATCAGAA 
GGAAACTCTG AAAGCAGTGC ACCTTTCCAC TCCATCCAGG CCAAAAAAAT ACAGCAAAAG 
GCATATGATG AGTTTGTAAG CCGTCTGCTT TCCCATGGAA GAGGTGCAAC ATTTTATGTA 
GAACCAAAAA GTGGGATGCT TGGCCCATTC GAGAGTCAAA CCATCAACAT CACTACTTTC 
ACAAATATGT GGGGGGATTA TCAGGACAAC CTCATATGCA AAGTTGGAGA TCTAAATCCA 
ACTCACATTC ATATGAAGAT GTCTGTGAGG GGCTGTCCAG TTTACTTTCA GATGATTGGA 
CCACAGCCTG ACAATCAGAA TCAGGGTCCA GTTATTAGAT TTGGAAGCCA TGTTTCTGGA 
GGAGACACTG TGTCACGCTC TTTACGACTC ATTAACACCA GTCCATATGA TATCCGTATG 
GACTGGGTGA CCTACAACCT GGAGGCTGGA GACGGCAAGC TGATAGATCT GCTGCTTGCA 
TGTGGTGAAC CTTTCCCTCT GAAGGATGCA GATGGCAATG AGGTTGTTGG AGGACTGGAC 
TCATCTGTCA TGTTCCCGTC CACATGGGAT GACAGTCACA CCCCCAGCAG AGAAGGAACA 
AGCTCAACTT TTATGACCCA ATCTGATGAT TTTGTAGAGA ATGGTGAAGA ATGTGATGGA 
GAAGATGGCA GTGGGGCTTC TGAGTCTCTA GCTCCAGTGA AGAAGCTGTT TTCTGTGTTT 
TTGAAGCCTC ATGAAGGGAC TCTTTCTGAC TACCCATACT GCATCACTCC ACAGCAGACT 
GTAGTTCCAG CAGGGGGCAG TAGCACCATT CATTTGTCTT TCACTCCCCT GATTCTGTCA 
TGTTCAACCA ATCAAAGATG TTTGGGTTAC GCTCTTGGTT TCATGAGCTT AGACTCTAAG 
CTTGCGTTAG TGACTCCAGG CAAGGTGGAG AGAGTCCGGG GCTATGAACT TCAACCTTTG 
AGGCTGGACA TGCAGGCTAA TGTAAAACCT GCCATTTTGT CAGTGCAGAT GGCAGAGGAT 
ACAGAAGTGC TGGAATTCAG CGCAGCTGCC AGCGACACAC TAGACGGAGC ATCACAAACA 
CAGGAATCCA GGATAACTCG CACATTCCGG CTAAAGAACG ACACAGACAT GGCCTTGAGT 
TTCAAACTGA GCACAAATCC ACCATTTTCA GTGCTGCTGC CCCGACAGAG CCTTAAACTG 
ATCAGCAGCT CGCCTGCTTA TAGACACACA CAAGGTCTGT CCGGACCAGG AGATGAGATG 
AGATTATTTC TGTTACAGCC CAAAAACATC ATTCAGGTTA AGGTGGCTTT CCACAATTCC 
GCTTCACTCC TGGCCTGTGT AAATGGAGCA TGTGAGGAGT CGGACAGTCA GCTCTCTGCT 
TCCCTTCTTT GCAGTGATGA TGGAGGAAAA ACTCTACAGT TTCAGGAGAA CTTGACCATT 
CAGTACAGCA ATAACAGCAT ACAGACAGTC TCTCTTTACG CTCGTCTGGC ATTACCTACA 
CTACACCTGT CCAGTGCCAC TGTGGATTTT GGCACCTGTT ATGTTGGACA AACAACTGTC 
AAAGAGATTT ATCTTCACAA CAGAGGTGGC TCCTGTAGCT ACTGGACCAC ACTCACAGAT 
GGCAATGAAG TGTTCAGGGT AAGTCCGGAT TCTGGTTTAC TGAAACCTCT TGGAGATCCA 
GCGTCCTGTA GACAGCTGCT GCAGATCAGC TTCACTCCGA GAGAACAGAA AGAGTTCCAG 
ACAATCTTCA CAGTGCAGGG GCTTCTTGGA GAGACACCAC TTGTTCTAAA GGTCAGCGGA 
AGAGGATCAT TTGATGAGAG TTTTGTATCT CCAATAACTG ACAGCTCACA AAACAACACT 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlec1 ( XM_017353956.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209445.1 Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X3, mRNA.

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CloneID	ODa57707
Clone ID Related Accession (Same CDS sequence)	XM_017353956.1 , XM_017353956.2
Accession Version	XM_017353956.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	deleted in lung and esophageal cancer protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353956.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATG AAACTGAACC AAAAGCACTA ACGTTAAAGA GTTCAGAACC GTCAATAAAC 
CGGCACACAC CAGCTTCAAA GACAACTCAG GACATTTCTC ATCTGCTGGC CAGCATATTC 
AATGATCTCT ACAGCACAGA GGTGATTGGG AAGGACACCG TTTCCAATCT CAGTAAATCA 
CGAAGAAGAG GCAACAGCTA CCATGAAAAA TATGTTGAGG AGCTACAACA GGTGCACAGT 
GAATACAACA GACGTATTCA GAATGCTGAT ATGCTAGAGA CACACATCAT ACAGGCCCGT 
CTTCAGGCTG CTGCAAAAGA AGAGCATGAT CGCAACAAAA TCCTGGAAGA AGTCGGTGAG 
GCTTACCAAC AGCTGGGACT TCCCCCAGTA AAATCAGCTT TCAAGTGGTG TGTGGACAGT 
GGACTTTTGA GGAGCAACAG CTTGATTTGC CCACTGGACT ACACAACAGT GCACAACCCA 
GTTGTTAAAA GCCCAAAAGC CAGGTCAAGT CCAGGCTTTG CCAAGCCTAC TTTCTCTTAT 
AATATGCACA TCTCCACTGA ACTGCAGGAT GATGGTTACA CTCCGCTTCC TCCACACGCT 
CATTCAGCAC ACAGCCTGCT TGAATATTCA GAGGAGACAA TCACACTCTC TTCCTCTCCA 
GAGTCCAGCT CCATCAATGA CACAGAGCTA GGTTTGGTGG CGAAGGCTTC TTGGATGGAG 
AAGACCAGTG CTAAGAGTCA TGCAGAAGAC AGGGCTTCCC TGAAAAAGCA CAAAGACCGG 
CATAAGTTTC TGCACAACCC TCGCTTCTTG CTGCCAAACA CTCAGCATGG AGGAAAGTCT 
CTCATCATCC CAGGGAAAAA GAAGCTGGAG AATGTGGGAA AGAGCAGGAA AAACAACGGC 
AGAGAGAGAA GTCTTGAGGG TCATGCGCCT ATTTTCACTG CAAATCCACC AGTGATCTTC 
TTCAGTGACT ACAGAGTAGG ACAAGTCTAT GAGACCTCAG TGGAGCTCAA AAACATGACT 
GCGAGCAGCC GTCACATTCG AATGATTCCT CCAACCTCCC CACACTTCTC TGTGGGTTTG 
GGCCGGTTCC CTGGTGAAGG AGGCATCGTG GCTCCAGGAA TGAGCTGTCA GTATATGGTG 
CGTTTTGCTC CAGATTCTCT AGCAGACTAT GAAGACGCTC TGGTTGTGGA GACGCAGATG 
CTCTATCCTC TCATTATACC AATAGAAGCC TGCAGATCTC CTCCGATACT CACCTTGCCG 
GCTGTCATGG ACTGTGGTTT CTGCCTGGTG GGAGGAGTGA AGTTTATGGA GGTTTTATGT 
CGGAATGAAG GACTGAGTGC TGGAACGTTT TGTGTAATGC CTAAGAAACA GTGGCCTGCT 
TCAAGCCTCA GGTCTGCAGT GAAGTCGACT TTCACAGAAC AGCCTCCCTT TGCTATCAGT 
CCCTCTATTT TCGGCCTTCA CCCTGGACAG GCTACTGTCA TAGAGGTTGT GTTTTTCCCC 
ACAACTCCTG AGCTCTCTAC TCAAGACTTC ACCATTGTCT GTGACAACTG CCAAGTGAAG 
GACATTACGC TCCAAGGTAC TGGTCAACTG ATCACTGTGG AGCTGCTTTC AGTATCAGGT 
GGAGAGGATC AGCCTGAATT TGCAGAATTG TGTGACATCA CAGCTGACCA TTATGTCAGA 
TTTAACACAA CCAATCCACA CTCTACCCTG CAGAAGACAC TGGTTGTCAA AAACAACACA 
CATTTGGAGC TTCCATATCA CTGGCAGATC ATGAAGCCCA GTCTTCAATG CTTGTTGCCC 
GGAGAGACTC CTGACCCTTC CAGCATTCAG CATCTTCTTG ATACAGACAG TGCATTCAGC 
ATCAGTCCTG TTATAGGAAT CCTGAACCCT GCACAGGAAC ACGAGTTCCT GCTCACCTAT 
GCTCCCAAAG ATATAAAAGA TTACCACAGT GTCTGCCACT TGGTGATTAT GGATGTTCCC 
AGTCCACCTA AAGTCTGTGA CGATGGGACA TGTCCTGATA CTGCACTCCA TGCAAACAAT 
GTGACCATTT TAGAGGTTGA AGTGAAAGGT TCTACTGAGC CATACAAGAT CCTTCTTGAG 
CCGTATGCTG TTTTAATACC AGGAGAGACC TATATACACA ACACAATTTG CAAGAGATTC 
AAGATGTGGA ATCACAGCAA ATCAGTCATT CACTTTGAAT GGGAACGTGT TATTGACAGT 
CATGTAATTG AAGTGGAGCC CTCATCTGGA GTAATAGAAA TGAATGAGTG TTTTGACATG 
GAGTTGTATC TGACTGGAAG GAGTCCGGGT CATTTTACCT CTACCCTGCA GTGTCACATT 
CTGCACCATC CTAAAACTAT AGGACTTGCC ATTGAGGCCA CATTTAAGGG TCCACATTGC 
TCATTGAATG TACCCAGTCT GGATTTTGGT TTGCTGCAAA TGGGAAATGA GAGAATTTTG 
ACTATTGAAG TCACCAACAA CAGCCTGCTA GATGCCCACT GGAGCCTGGA GGTGCTGTCC 
CAAACCCAGA AGCTCATCGA TGGACAGGTG CATATAAATC CAAATAAAGG TGTTCTGGCT 
GCACTAGAGA GCTGCAGTGT GGATGTGGCC TTCAGAGCCA TGCATTGCCA AGTATTTGAG 
TCAGTTTTAC AACTTTCTGT GCTGAACGGC ACCGGTTGTC ACGTCTCCAT CCATGCCGTT 
GTTCAGTCTC CTCAAGTGTG CTTGCTGAAC TGTGAGTTGC TGTTTGCTGA TCTGTATGTT 
GGGGTTTCAC AGACGGGCAG TGTCACTCTG TTTAACCAAA CACTACTGCC AGCTCACTTC 
ACCTGGGGCA GGCTTCAAGG TTCACAAGCT CATCTCTGCT CCGCAGTCTT CAGTCCCTCT 
GCAGGATCTC TGGGGCCCAA TGCAAAAACT GAAGTCTCTG TGTCATTCAC AGCTCACACT 
GTTGAGGATT TGACTGAAGT GAATGGAGTT TGTGAGGTAA AAGGAATGGA GAATCCACTT 
GTGCTGGGAA TTTTTTCTAA AGCCAAACCT CTCAGAGTTT CCTACTCACT GCTTTGTAAT 
GGTATTAATT CAGGTGATGG CGATCACCAA CCAATCCTGC TGGACTTCAC TGAGCATGAG 
CCAGCCGTCA TTGGGAAATC TGTCAAACGG CAGTTACTGA TAACTAACAA CACTGCTATT 
ACTGCTCCAT TCACTGTCGA CACTGAGTAC TTCACTGGAG ACTGTCCTTC ACAATCAGAA 
GGAAACTCTG AAAGCAGTGC ACCTTTCCAC TCCATCCAGG CCAAAAAAAT ACAGCAAAAG 
GCATATGATG AGTTTGTAAG CCGTCTGCTT TCCCATGGAA GAGGTGCAAC ATTTTATGTA 
GAACCAAAAA GTGGGATGCT TGGCCCATTC GAGAGTCAAA CCATCAACAT CACTACTTTC 
ACAAATATGT GGGGGGATTA TCAGGACAAC CTCATATGCA AAGTTGGAGA TCTAAATCCA 
ACTCACATTC ATATGAAGAT GTCTGTGAGG GGCTGTCCAG TTTACTTTCA GATGATTGGA 
CCACAGCCTG ACAATCAGAA TCAGGGTCCA GTTATTAGAT TTGGAAGCCA TGTTTCTGGA 
GGAGACACTG TGTCACGCTC TTTACGACTC ATTAACACCA GTCCATATGA TATCCGTATG 
GACTGGGTGA CCTACAACCT GGAGGCTGGA GACGGCAAGC TGATAGATCT GCTGCTTGCA 
TGTGGTGAAC CTTTCCCTCT GAAGGATGCA GATGGCAATG AGGTTGTTGG AGGACTGGAC 
TCATCTGTCA TGTTCCCGTC CACATGGGAT GACAGTCACA CCCCCAGCAG AGAAGGAACA 
AGCTCAACTT TTATGACCCA ATCTGATGAT TTTGTAGAGA ATGGTGAAGA ATGTGATGGA 
GAAGATGGCA GTGGGGCTTC TGAGTCTCTA GCTCCAGTGA AGAAGCTGTT TTCTGTGTTT 
TTGAAGCCTC ATGAAGGGAC TCTTTCTGAC TACCCATACT GCATCACTCC ACAGCAGACT 
GTAGTTCCAG CAGGGGGCAG TAGCACCATT CATTTGTCTT TCACTCCCCT GATTCTGTCA 
TGTTCAACCA ATCAAAGATG TTTGGGTTAC GCTCTTGGTT TCATGAGCTT AGACTCTAAG 
CTTGCGTTAG TGACTCCAGG CAAGGTGGAG AGAGTCCGGG GCTATGAACT TCAACCTTTG 
AGGCTGGACA TGCAGGCTAA TGTAAAACCT GCCATTTTGT CAGTGCAGAT GGCAGAGGAT 
ACAGAAGTGC TGGAATTCAG CGCAGCTGCC AGCGACACAC TAGACGGAGC ATCACAAACA 
CAGGAATCCA GGATAACTCG CACATTCCGG CTAAAGAACG ACACAGACAT GGCCTTGAGT 
TTCAAACTGA GCACAAATCC ACCATTTTCA GTGCTGCTGC CCCGACAGAG CCTTAAACTG 
ATCAGCAGCT CGCCTGCTTA TAGACACACA CAAGGTCTGT CCGGACCAGG AGATGAGATG 
AGATTATTTC TGTTACAGCC CAAAAACATC ATTCAGGTTA AGGTGGCTTT CCACAATTCC 
GCTTCACTCC TGGCCTGTGT AAATGGAGCA TGTGAGGAGT CGGACAGTCA GCTCTCTGCT 
TCCCTTCTTT GCAGTGATGA TGGAGGAAAA ACTCTACAGT TTCAGGAGAA CTTGACCATT 
CAGTACAGCA ATAACAGCAT ACAGACAGTC TCTCTTTACG CTCGTCTGGC ATTACCTACA 
CTACACCTGT CCAGTGCCAC TGTGGATTTT GGCACCTGTT ATGTTGGACA AACAACTGTC 
AAAGAGATTT ATCTTCACAA CAGAGGTGGC TCCTGTAGCT ACTGGACCAC ACTCACAGAT 
GGCAATGAAG TGTTCAGGGT AAGTCCGGAT TCTGGTTTAC TGAAACCTCT TGGAGATCCA 
GCGTCCTGTA GACAGCTGCT GCAGATCAGC TTCACTCCGA GAGAACAGAA AGAGTTCCAG 
ACAATCTTCA CAGTGCAGGG GCTTCTTGGA GAGACACCAC TTGTTCTAAA GGTCAGCGGA 
AGAGGATCAT TTGATGAGAG TTTTGTATCT CCAATAACTG ACAGCTCACA AAACAACACT 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209445.1
CDS	197..5302
Translation	MADETEPKALTLKSSEPSINRHTPASKTTQDISHLLASIFNDLYSTEVIGKDTVSNLSKSRRRGNSYHEKYVEELQQVHSEYNRRIQNADMLETHIIQARLQAAAKEEHDRNKILEEVGEAYQQLGLPPVKSAFKWCVDSGLLRSNSLICPLDYTTVHNPVVKSPKARSSPGFAKPTFSYNMHISTELQDDGYTPLPPHAHSAHSLLEYSEETITLSSSPESSSINDTELGLVAKASWMEKTSAKSHAEDRASLKKHKDRHKFLHNPRFLLPNTQHGGKSLIIPGKKKLENVGKSRKNNGRERSLEGHAPIFTANPPVIFFSDYRVGQVYETSVELKNMTASSRHIRMIPPTSPHFSVGLGRFPGEGGIVAPGMSCQYMVRFAPDSLADYEDALVVETQMLYPLIIPIEACRSPPILTLPAVMDCGFCLVGGVKFMEVLCRNEGLSAGTFCVMPKKQWPASSLRSAVKSTFTEQPPFAISPSIFGLHPGQATVIEVVFFPTTPELSTQDFTIVCDNCQVKDITLQGTGQLITVELLSVSGGEDQPEFAELCDITADHYVRFNTTNPHSTLQKTLVVKNNTHLELPYHWQIMKPSLQCLLPGETPDPSSIQHLLDTDSAFSISPVIGILNPAQEHEFLLTYAPKDIKDYHSVCHLVIMDVPSPPKVCDDGTCPDTALHANNVTILEVEVKGSTEPYKILLEPYAVLIPGETYIHNTICKRFKMWNHSKSVIHFEWERVIDSHVIEVEPSSGVIEMNECFDMELYLTGRSPGHFTSTLQCHILHHPKTIGLAIEATFKGPHCSLNVPSLDFGLLQMGNERILTIEVTNNSLLDAHWSLEVLSQTQKLIDGQVHINPNKGVLAALESCSVDVAFRAMHCQVFESVLQLSVLNGTGCHVSIHAVVQSPQVCLLNCELLFADLYVGVSQTGSVTLFNQTLLPAHFTWGRLQGSQAHLCSAVFSPSAGSLGPNAKTEVSVSFTAHTVEDLTEVNGVCEVKGMENPLVLGIFSKAKPLRVSYSLLCNGINSGDGDHQPILLDFTEHEPAVIGKSVKRQLLITNNTAITAPFTVDTEYFTGDCPSQSEGNSESSAPFHSIQAKKIQQKAYDEFVSRLLSHGRGATFYVEPKSGMLGPFESQTINITTFTNMWGDYQDNLICKVGDLNPTHIHMKMSVRGCPVYFQMIGPQPDNQNQGPVIRFGSHVSGGDTVSRSLRLINTSPYDIRMDWVTYNLEAGDGKLIDLLLACGEPFPLKDADGNEVVGGLDSSVMFPSTWDDSHTPSREGTSSTFMTQSDDFVENGEECDGEDGSGASESLAPVKKLFSVFLKPHEGTLSDYPYCITPQQTVVPAGGSSTIHLSFTPLILSCSTNQRCLGYALGFMSLDSKLALVTPGKVERVRGYELQPLRLDMQANVKPAILSVQMAEDTEVLEFSAAASDTLDGASQTQESRITRTFRLKNDTDMALSFKLSTNPPFSVLLPRQSLKLISSSPAYRHTQGLSGPGDEMRLFLLQPKNIIQVKVAFHNSASLLACVNGACEESDSQLSASLLCSDDGGKTLQFQENLTIQYSNNSIQTVSLYARLALPTLHLSSATVDFGTCYVGQTTVKEIYLHNRGGSCSYWTTLTDGNEVFRVSPDSGLLKPLGDPASCRQLLQISFTPREQKEFQTIFTVQGLLGETPLVLKVSGRGSFDESFVSPITDSSQNNTE

Target ORF information:

RefSeq Version	XM_017353956.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353956.2

ORF Insert Sequence:

ATGGCTGATG AAACTGAACC AAAAGCACTA ACGTTAAAGA GTTCAGAACC GTCAATAAAC 
CGGCACACAC CAGCTTCAAA GACAACTCAG GACATTTCTC ATCTGCTGGC CAGCATATTC 
AATGATCTCT ACAGCACAGA GGTGATTGGG AAGGACACCG TTTCCAATCT CAGTAAATCA 
CGAAGAAGAG GCAACAGCTA CCATGAAAAA TATGTTGAGG AGCTACAACA GGTGCACAGT 
GAATACAACA GACGTATTCA GAATGCTGAT ATGCTAGAGA CACACATCAT ACAGGCCCGT 
CTTCAGGCTG CTGCAAAAGA AGAGCATGAT CGCAACAAAA TCCTGGAAGA AGTCGGTGAG 
GCTTACCAAC AGCTGGGACT TCCCCCAGTA AAATCAGCTT TCAAGTGGTG TGTGGACAGT 
GGACTTTTGA GGAGCAACAG CTTGATTTGC CCACTGGACT ACACAACAGT GCACAACCCA 
GTTGTTAAAA GCCCAAAAGC CAGGTCAAGT CCAGGCTTTG CCAAGCCTAC TTTCTCTTAT 
AATATGCACA TCTCCACTGA ACTGCAGGAT GATGGTTACA CTCCGCTTCC TCCACACGCT 
CATTCAGCAC ACAGCCTGCT TGAATATTCA GAGGAGACAA TCACACTCTC TTCCTCTCCA 
GAGTCCAGCT CCATCAATGA CACAGAGCTA GGTTTGGTGG CGAAGGCTTC TTGGATGGAG 
AAGACCAGTG CTAAGAGTCA TGCAGAAGAC AGGGCTTCCC TGAAAAAGCA CAAAGACCGG 
CATAAGTTTC TGCACAACCC TCGCTTCTTG CTGCCAAACA CTCAGCATGG AGGAAAGTCT 
CTCATCATCC CAGGGAAAAA GAAGCTGGAG AATGTGGGAA AGAGCAGGAA AAACAACGGC 
AGAGAGAGAA GTCTTGAGGG TCATGCGCCT ATTTTCACTG CAAATCCACC AGTGATCTTC 
TTCAGTGACT ACAGAGTAGG ACAAGTCTAT GAGACCTCAG TGGAGCTCAA AAACATGACT 
GCGAGCAGCC GTCACATTCG AATGATTCCT CCAACCTCCC CACACTTCTC TGTGGGTTTG 
GGCCGGTTCC CTGGTGAAGG AGGCATCGTG GCTCCAGGAA TGAGCTGTCA GTATATGGTG 
CGTTTTGCTC CAGATTCTCT AGCAGACTAT GAAGACGCTC TGGTTGTGGA GACGCAGATG 
CTCTATCCTC TCATTATACC AATAGAAGCC TGCAGATCTC CTCCGATACT CACCTTGCCG 
GCTGTCATGG ACTGTGGTTT CTGCCTGGTG GGAGGAGTGA AGTTTATGGA GGTTTTATGT 
CGGAATGAAG GACTGAGTGC TGGAACGTTT TGTGTAATGC CTAAGAAACA GTGGCCTGCT 
TCAAGCCTCA GGTCTGCAGT GAAGTCGACT TTCACAGAAC AGCCTCCCTT TGCTATCAGT 
CCCTCTATTT TCGGCCTTCA CCCTGGACAG GCTACTGTCA TAGAGGTTGT GTTTTTCCCC 
ACAACTCCTG AGCTCTCTAC TCAAGACTTC ACCATTGTCT GTGACAACTG CCAAGTGAAG 
GACATTACGC TCCAAGGTAC TGGTCAACTG ATCACTGTGG AGCTGCTTTC AGTATCAGGT 
GGAGAGGATC AGCCTGAATT TGCAGAATTG TGTGACATCA CAGCTGACCA TTATGTCAGA 
TTTAACACAA CCAATCCACA CTCTACCCTG CAGAAGACAC TGGTTGTCAA AAACAACACA 
CATTTGGAGC TTCCATATCA CTGGCAGATC ATGAAGCCCA GTCTTCAATG CTTGTTGCCC 
GGAGAGACTC CTGACCCTTC CAGCATTCAG CATCTTCTTG ATACAGACAG TGCATTCAGC 
ATCAGTCCTG TTATAGGAAT CCTGAACCCT GCACAGGAAC ACGAGTTCCT GCTCACCTAT 
GCTCCCAAAG ATATAAAAGA TTACCACAGT GTCTGCCACT TGGTGATTAT GGATGTTCCC 
AGTCCACCTA AAGTCTGTGA CGATGGGACA TGTCCTGATA CTGCACTCCA TGCAAACAAT 
GTGACCATTT TAGAGGTTGA AGTGAAAGGT TCTACTGAGC CATACAAGAT CCTTCTTGAG 
CCGTATGCTG TTTTAATACC AGGAGAGACC TATATACACA ACACAATTTG CAAGAGATTC 
AAGATGTGGA ATCACAGCAA ATCAGTCATT CACTTTGAAT GGGAACGTGT TATTGACAGT 
CATGTAATTG AAGTGGAGCC CTCATCTGGA GTAATAGAAA TGAATGAGTG TTTTGACATG 
GAGTTGTATC TGACTGGAAG GAGTCCGGGT CATTTTACCT CTACCCTGCA GTGTCACATT 
CTGCACCATC CTAAAACTAT AGGACTTGCC ATTGAGGCCA CATTTAAGGG TCCACATTGC 
TCATTGAATG TACCCAGTCT GGATTTTGGT TTGCTGCAAA TGGGAAATGA GAGAATTTTG 
ACTATTGAAG TCACCAACAA CAGCCTGCTA GATGCCCACT GGAGCCTGGA GGTGCTGTCC 
CAAACCCAGA AGCTCATCGA TGGACAGGTG CATATAAATC CAAATAAAGG TGTTCTGGCT 
GCACTAGAGA GCTGCAGTGT GGATGTGGCC TTCAGAGCCA TGCATTGCCA AGTATTTGAG 
TCAGTTTTAC AACTTTCTGT GCTGAACGGC ACCGGTTGTC ACGTCTCCAT CCATGCCGTT 
GTTCAGTCTC CTCAAGTGTG CTTGCTGAAC TGTGAGTTGC TGTTTGCTGA TCTGTATGTT 
GGGGTTTCAC AGACGGGCAG TGTCACTCTG TTTAACCAAA CACTACTGCC AGCTCACTTC 
ACCTGGGGCA GGCTTCAAGG TTCACAAGCT CATCTCTGCT CCGCAGTCTT CAGTCCCTCT 
GCAGGATCTC TGGGGCCCAA TGCAAAAACT GAAGTCTCTG TGTCATTCAC AGCTCACACT 
GTTGAGGATT TGACTGAAGT GAATGGAGTT TGTGAGGTAA AAGGAATGGA GAATCCACTT 
GTGCTGGGAA TTTTTTCTAA AGCCAAACCT CTCAGAGTTT CCTACTCACT GCTTTGTAAT 
GGTATTAATT CAGGTGATGG CGATCACCAA CCAATCCTGC TGGACTTCAC TGAGCATGAG 
CCAGCCGTCA TTGGGAAATC TGTCAAACGG CAGTTACTGA TAACTAACAA CACTGCTATT 
ACTGCTCCAT TCACTGTCGA CACTGAGTAC TTCACTGGAG ACTGTCCTTC ACAATCAGAA 
GGAAACTCTG AAAGCAGTGC ACCTTTCCAC TCCATCCAGG CCAAAAAAAT ACAGCAAAAG 
GCATATGATG AGTTTGTAAG CCGTCTGCTT TCCCATGGAA GAGGTGCAAC ATTTTATGTA 
GAACCAAAAA GTGGGATGCT TGGCCCATTC GAGAGTCAAA CCATCAACAT CACTACTTTC 
ACAAATATGT GGGGGGATTA TCAGGACAAC CTCATATGCA AAGTTGGAGA TCTAAATCCA 
ACTCACATTC ATATGAAGAT GTCTGTGAGG GGCTGTCCAG TTTACTTTCA GATGATTGGA 
CCACAGCCTG ACAATCAGAA TCAGGGTCCA GTTATTAGAT TTGGAAGCCA TGTTTCTGGA 
GGAGACACTG TGTCACGCTC TTTACGACTC ATTAACACCA GTCCATATGA TATCCGTATG 
GACTGGGTGA CCTACAACCT GGAGGCTGGA GACGGCAAGC TGATAGATCT GCTGCTTGCA 
TGTGGTGAAC CTTTCCCTCT GAAGGATGCA GATGGCAATG AGGTTGTTGG AGGACTGGAC 
TCATCTGTCA TGTTCCCGTC CACATGGGAT GACAGTCACA CCCCCAGCAG AGAAGGAACA 
AGCTCAACTT TTATGACCCA ATCTGATGAT TTTGTAGAGA ATGGTGAAGA ATGTGATGGA 
GAAGATGGCA GTGGGGCTTC TGAGTCTCTA GCTCCAGTGA AGAAGCTGTT TTCTGTGTTT 
TTGAAGCCTC ATGAAGGGAC TCTTTCTGAC TACCCATACT GCATCACTCC ACAGCAGACT 
GTAGTTCCAG CAGGGGGCAG TAGCACCATT CATTTGTCTT TCACTCCCCT GATTCTGTCA 
TGTTCAACCA ATCAAAGATG TTTGGGTTAC GCTCTTGGTT TCATGAGCTT AGACTCTAAG 
CTTGCGTTAG TGACTCCAGG CAAGGTGGAG AGAGTCCGGG GCTATGAACT TCAACCTTTG 
AGGCTGGACA TGCAGGCTAA TGTAAAACCT GCCATTTTGT CAGTGCAGAT GGCAGAGGAT 
ACAGAAGTGC TGGAATTCAG CGCAGCTGCC AGCGACACAC TAGACGGAGC ATCACAAACA 
CAGGAATCCA GGATAACTCG CACATTCCGG CTAAAGAACG ACACAGACAT GGCCTTGAGT 
TTCAAACTGA GCACAAATCC ACCATTTTCA GTGCTGCTGC CCCGACAGAG CCTTAAACTG 
ATCAGCAGCT CGCCTGCTTA TAGACACACA CAAGGTCTGT CCGGACCAGG AGATGAGATG 
AGATTATTTC TGTTACAGCC CAAAAACATC ATTCAGGTTA AGGTGGCTTT CCACAATTCC 
GCTTCACTCC TGGCCTGTGT AAATGGAGCA TGTGAGGAGT CGGACAGTCA GCTCTCTGCT 
TCCCTTCTTT GCAGTGATGA TGGAGGAAAA ACTCTACAGT TTCAGGAGAA CTTGACCATT 
CAGTACAGCA ATAACAGCAT ACAGACAGTC TCTCTTTACG CTCGTCTGGC ATTACCTACA 
CTACACCTGT CCAGTGCCAC TGTGGATTTT GGCACCTGTT ATGTTGGACA AACAACTGTC 
AAAGAGATTT ATCTTCACAA CAGAGGTGGC TCCTGTAGCT ACTGGACCAC ACTCACAGAT 
GGCAATGAAG TGTTCAGGGT AAGTCCGGAT TCTGGTTTAC TGAAACCTCT TGGAGATCCA 
GCGTCCTGTA GACAGCTGCT GCAGATCAGC TTCACTCCGA GAGAACAGAA AGAGTTCCAG 
ACAATCTTCA CAGTGCAGGG GCTTCTTGGA GAGACACCAC TTGTTCTAAA GGTCAGCGGA 
AGAGGATCAT TTGATGAGAG TTTTGTATCT CCAATAACTG ACAGCTCACA AAACAACACT 
GAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlec1 ( XM_017353957.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209446.1 Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X4, mRNA.

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CloneID	ODa57708
Clone ID Related Accession (Same CDS sequence)	XM_017353957.1
Accession Version	XM_017353957.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4839bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	deleted in lung and esophageal cancer protein 1 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTAGAGA CACACATCAT ACAGGCCCGT CTTCAGGCTG CTGCAAAAGA AGAGCATGAT 
CGCAACAAAA TCCTGGAAGA AGTCGGTGAG GCTTACCAAC AGCTGGGACT TCCCCCAGTA 
AAATCAGCTT TCAAGTGGTG TGTGGACAGT GGACTTTTGA GGAGCAACAG CTTGATTTGC 
CCACTGGACT ACACAACAGT GCACAACCCA GTTGTTAAAA GCCCAAAAGC CAGGTCAAGT 
CCAGGCTTTG CCAAGCCTAC TTTCTCTTAT AATATGCACA TCTCCACTGA ACTGCAGGAT 
GATGGTTACA CTCCGCTTCC TCCACACGCT CATTCAGCAC ACAGCCTGCT TGAATATTCA 
GAGGAGACAA TCACACTCTC TTCCTCTCCA GAGTCCAGCT CCATCAATGA CACAGAGCTA 
GGTTTGGTGG CGAAGGCTTC TTGGATGGAG AAGACCAGTG CTAAGAGTCA TGCAGAAGAC 
AGGGCTTCCC TGAAAAAGCA CAAAGACCGG CATAAGTTTC TGCACAACCC TCGCTTCTTG 
CTGCCAAACA CTCAGCATGG AGGAAAGTCT CTCATCATCC CAGGGAAAAA GAAGCTGGAG 
AATGTGGGAA AGAGCAGGAA AAACAACGGC AGAGAGAGAA GTCTTGAGGG TCATGCGCCT 
ATTTTCACTG CAAATCCACC AGTGATCTTC TTCAGTGACT ACAGAGTAGG ACAAGTCTAT 
GAGACCTCAG TGGAGCTCAA AAACATGACT GCGAGCAGCC GTCACATTCG AATGATTCCT 
CCAACCTCCC CACACTTCTC TGTGGGTTTG GGCCGGTTCC CTGGTGAAGG AGGCATCGTG 
GCTCCAGGAA TGAGCTGTCA GTATATGGTG CGTTTTGCTC CAGATTCTCT AGCAGACTAT 
GAAGACGCTC TGGTTGTGGA GACGCAGATG CTCTATCCTC TCATTATACC AATAGAAGCC 
TGCAGATCTC CTCCGATACT CACCTTGCCG GCTGTCATGG ACTGTGGTTT CTGCCTGGTG 
GGAGGAGTGA AGTTTATGGA GGTTTTATGT CGGAATGAAG GACTGAGTGC TGGAACGTTT 
TGTGTAATGC CTAAGAAACA GTGGCCTGCT TCAAGCCTCA GGTCTGCAGT GAAGTCGACT 
TTCACAGAAC AGCCTCCCTT TGCTATCAGT CCCTCTATTT TCGGCCTTCA CCCTGGACAG 
GCTACTGTCA TAGAGGTTGT GTTTTTCCCC ACAACTCCTG AGCTCTCTAC TCAAGACTTC 
ACCATTGTCT GTGACAACTG CCAAGTGAAG GACATTACGC TCCAAGGTAC TGGTCAACTG 
ATCACTGTGG AGCTGCTTTC AGTATCAGGT GGAGAGGATC AGCCTGAATT TGCAGAATTG 
TGTGACATCA CAGCTGACCA TTATGTCAGA TTTAACACAA CCAATCCACA CTCTACCCTG 
CAGAAGACAC TGGTTGTCAA AAACAACACA CATTTGGAGC TTCCATATCA CTGGCAGATC 
ATGAAGCCCA GTCTTCAATG CTTGTTGCCC GGAGAGACTC CTGACCCTTC CAGCATTCAG 
CATCTTCTTG ATACAGACAG TGCATTCAGC ATCAGTCCTG TTATAGGAAT CCTGAACCCT 
GCACAGGAAC ACGAGTTCCT GCTCACCTAT GCTCCCAAAG ATATAAAAGA TTACCACAGT 
GTCTGCCACT TGGTGATTAT GGATGTTCCC AGTCCACCTA AAGTCTGTGA CGATGGGACA 
TGTCCTGATA CTGCACTCCA TGCAAACAAT GTGACCATTT TAGAGGTTGA AGTGAAAGGT 
TCTACTGAGC CATACAAGAT CCTTCTTGAG CCGTATGCTG TTTTAATACC AGGAGAGACC 
TATATACACA ACACAATTTG CAAGAGATTC AAGATGTGGA ATCACAGCAA ATCAGTCATT 
CACTTTGAAT GGGAACGTGT TATTGACAGT CATGTAATTG AAGTGGAGCC CTCATCTGGA 
GTAATAGAAA TGAATGAGTG TTTTGACATG GAGTTGTATC TGACTGGAAG GAGTCCGGGT 
CATTTTACCT CTACCCTGCA GTGTCACATT CTGCACCATC CTAAAACTAT AGGACTTGCC 
ATTGAGGCCA CATTTAAGGG TCCACATTGC TCATTGAATG TACCCAGTCT GGATTTTGGT 
TTGCTGCAAA TGGGAAATGA GAGAATTTTG ACTATTGAAG TCACCAACAA CAGCCTGCTA 
GATGCCCACT GGAGCCTGGA GGTGCTGTCC CAAACCCAGA AGCTCATCGA TGGACAGGTG 
CATATAAATC CAAATAAAGG TGTTCTGGCT GCACTAGAGA GCTGCAGTGT GGATGTGGCC 
TTCAGAGCCA TGCATTGCCA AGTATTTGAG TCAGTTTTAC AACTTTCTGT GCTGAACGGC 
ACCGGTTGTC ACGTCTCCAT CCATGCCGTT GTTCAGTCTC CTCAAGTGTG CTTGCTGAAC 
TGTGAGTTGC TGTTTGCTGA TCTGTATGTT GGGGTTTCAC AGACGGGCAG TGTCACTCTG 
TTTAACCAAA CACTACTGCC AGCTCACTTC ACCTGGGGCA GGCTTCAAGG TTCACAAGCT 
CATCTCTGCT CCGCAGTCTT CAGTCCCTCT GCAGGATCTC TGGGGCCCAA TGCAAAAACT 
GAAGTCTCTG TGTCATTCAC AGCTCACACT GTTGAGGATT TGACTGAAGT GAATGGAGTT 
TGTGAGGTAA AAGGAATGGA GAATCCACTT GTGCTGGGAA TTTTTTCTAA AGCCAAACCT 
CTCAGAGTTT CCTACTCACT GCTTTGTAAT GGTATTAATT CAGGTGATGG CGATCACCAA 
CCAATCCTGC TGGACTTCAC TGAGCATGAG CCAGCCGTCA TTGGGAAATC TGTCAAACGG 
CAGTTACTGA TAACTAACAA CACTGCTATT ACTGCTCCAT TCACTGTCGA CACTGAGTAC 
TTCACTGGAG ACTGTCCTTC ACAATCAGAA GGAAACTCTG AAAGAAGCAG TGCACCTTTC 
CACTCCATCC AGGCCAAAAA AATACAGCAA AAGGCATATG ATGAGTTTGT AAGCCGTCTG 
CTTTCCCATG GAAGAGGTGC AACATTTTAT GTAGAACCAA AAAGTGGGAT GCTTGGCCCA 
TTCGAGAGTC AAACCATCAA CATCACTACT TTCACAAATA TGTGGGGGGA TTATCAGGAC 
AACCTCATAT GCAAAGTTGG AGATCTAAAT CCAACTCACA TTCATATGAA GATGTCTGTG 
AGGGGCTGTC CAGTTTACTT TCAGATGATT GGACCACAGC CTGACAATCA GAATCAGGGT 
CCAGTTATTA GATTTGGAAG CCATGTTTCT GGAGGAGACA CTGTGTCACG CTCTTTACGA 
CTCATTAACA CCAGTCCATA TGATATCCGT ATGGACTGGG TGACCTACAA CCTGGAGGCT 
GGAGACGGCA AGCTGATAGA TCTGCTGCTT GCATGTGGTG AACCTTTCCC TCTGAAGGAT 
GCAGATGGCA ATGAGGTTGT TGGAGGACTG GACTCATCTG TCATGTTCCC GTCCACATGG 
GATGACAGTC ACACCCCCAG CAGAGAAGGA ACAAGCTCAA CTTTTATGAC CCAATCTGAT 
GATTTTGTAG AGAATGGTGA AGAATGTGAT GGAGAAGATG GCAGTGGGGC TTCTGAGTCT 
CTAGCTCCAG TGAAGAAGCT GTTTTCTGTG TTTTTGAAGC CTCATGAAGG GACTCTTTCT 
GACTACCCAT ACTGCATCAC TCCACAGCAG ACTGTAGTTC CAGCAGGGGG CAGTAGCACC 
ATTCATTTGT CTTTCACTCC CCTGATTCTG TCATGTTCAA CCAATCAAAG ATGTTTGGGT 
TACGCTCTTG GTTTCATGAG CTTAGACTCT AAGCTTGCGT TAGTGACTCC AGGCAAGGTG 
GAGAGAGTCC GGGGCTATGA ACTTCAACCT TTGAGGCTGG ACATGCAGGC TAATGTAAAA 
CCTGCCATTT TGTCAGTGCA GATGGCAGAG GATACAGAAG TGCTGGAATT CAGCGCAGCT 
GCCAGCGACA CACTAGACGG AGCATCACAA ACACAGGAAT CCAGGATAAC TCGCACATTC 
CGGCTAAAGA ACGACACAGA CATGGCCTTG AGTTTCAAAC TGAGCACAAA TCCACCATTT 
TCAGTGCTGC TGCCCCGACA GAGCCTTAAA CTGATCAGCA GCTCGCCTGC TTATAGACAC 
ACACAAGGTC TGTCCGGACC AGGAGATGAG ATGAGATTAT TTCTGTTACA GCCCAAAAAC 
ATCATTCAGG TTAAGGTGGC TTTCCACAAT TCCGCTTCAC TCCTGGCCTG TGTAAATGGA 
GCATGTGAGG AGTCGGACAG TCAGCTCTCT GCTTCCCTTC TTTGCAGTGA TGATGGAGGA 
AAAACTCTAC AGTTTCAGGA GAACTTGACC ATTCAGTACA GCAATAACAG CATACAGACA 
GTCTCTCTTT ACGCTCGTCT GGCATTACCT ACACTACACC TGTCCAGTGC CACTGTGGAT 
TTTGGCACCT GTTATGTTGG ACAAACAACT GTCAAAGAGA TTTATCTTCA CAACAGAGGT 
GGCTCCTGTA GCTACTGGAC CACACTCACA GATGGCAATG AAGTGTTCAG GGTAAGTCCG 
GATTCTGGTT TACTGAAACC TCTTGGAGAT CCAGCGTCCT GTAGACAGCT GCTGCAGATC 
AGCTTCACTC CGAGAGAACA GAAAGAGTTC CAGACAATCT TCACAGTGCA GGGGCTTCTT 
GGAGAGACAC CACTTGTTCT AAAGGTCAGC GGAAGAGGAT CATTTGATGA GAGTTTTGTA 
TCTCCAATAA CTGACAGCTC ACAAAACAAC ACTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209446.1
CDS	338..5176
Translation	MLETHIIQARLQAAAKEEHDRNKILEEVGEAYQQLGLPPVKSAFKWCVDSGLLRSNSLICPLDYTTVHNPVVKSPKARSSPGFAKPTFSYNMHISTELQDDGYTPLPPHAHSAHSLLEYSEETITLSSSPESSSINDTELGLVAKASWMEKTSAKSHAEDRASLKKHKDRHKFLHNPRFLLPNTQHGGKSLIIPGKKKLENVGKSRKNNGRERSLEGHAPIFTANPPVIFFSDYRVGQVYETSVELKNMTASSRHIRMIPPTSPHFSVGLGRFPGEGGIVAPGMSCQYMVRFAPDSLADYEDALVVETQMLYPLIIPIEACRSPPILTLPAVMDCGFCLVGGVKFMEVLCRNEGLSAGTFCVMPKKQWPASSLRSAVKSTFTEQPPFAISPSIFGLHPGQATVIEVVFFPTTPELSTQDFTIVCDNCQVKDITLQGTGQLITVELLSVSGGEDQPEFAELCDITADHYVRFNTTNPHSTLQKTLVVKNNTHLELPYHWQIMKPSLQCLLPGETPDPSSIQHLLDTDSAFSISPVIGILNPAQEHEFLLTYAPKDIKDYHSVCHLVIMDVPSPPKVCDDGTCPDTALHANNVTILEVEVKGSTEPYKILLEPYAVLIPGETYIHNTICKRFKMWNHSKSVIHFEWERVIDSHVIEVEPSSGVIEMNECFDMELYLTGRSPGHFTSTLQCHILHHPKTIGLAIEATFKGPHCSLNVPSLDFGLLQMGNERILTIEVTNNSLLDAHWSLEVLSQTQKLIDGQVHINPNKGVLAALESCSVDVAFRAMHCQVFESVLQLSVLNGTGCHVSIHAVVQSPQVCLLNCELLFADLYVGVSQTGSVTLFNQTLLPAHFTWGRLQGSQAHLCSAVFSPSAGSLGPNAKTEVSVSFTAHTVEDLTEVNGVCEVKGMENPLVLGIFSKAKPLRVSYSLLCNGINSGDGDHQPILLDFTEHEPAVIGKSVKRQLLITNNTAITAPFTVDTEYFTGDCPSQSEGNSERSSAPFHSIQAKKIQQKAYDEFVSRLLSHGRGATFYVEPKSGMLGPFESQTINITTFTNMWGDYQDNLICKVGDLNPTHIHMKMSVRGCPVYFQMIGPQPDNQNQGPVIRFGSHVSGGDTVSRSLRLINTSPYDIRMDWVTYNLEAGDGKLIDLLLACGEPFPLKDADGNEVVGGLDSSVMFPSTWDDSHTPSREGTSSTFMTQSDDFVENGEECDGEDGSGASESLAPVKKLFSVFLKPHEGTLSDYPYCITPQQTVVPAGGSSTIHLSFTPLILSCSTNQRCLGYALGFMSLDSKLALVTPGKVERVRGYELQPLRLDMQANVKPAILSVQMAEDTEVLEFSAAASDTLDGASQTQESRITRTFRLKNDTDMALSFKLSTNPPFSVLLPRQSLKLISSSPAYRHTQGLSGPGDEMRLFLLQPKNIIQVKVAFHNSASLLACVNGACEESDSQLSASLLCSDDGGKTLQFQENLTIQYSNNSIQTVSLYARLALPTLHLSSATVDFGTCYVGQTTVKEIYLHNRGGSCSYWTTLTDGNEVFRVSPDSGLLKPLGDPASCRQLLQISFTPREQKEFQTIFTVQGLLGETPLVLKVSGRGSFDESFVSPITDSSQNNTE

Target ORF information:

RefSeq Version	XM_017353957.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353957.1

ORF Insert Sequence:

ATGCTAGAGA CACACATCAT ACAGGCCCGT CTTCAGGCTG CTGCAAAAGA AGAGCATGAT 
CGCAACAAAA TCCTGGAAGA AGTCGGTGAG GCTTACCAAC AGCTGGGACT TCCCCCAGTA 
AAATCAGCTT TCAAGTGGTG TGTGGACAGT GGACTTTTGA GGAGCAACAG CTTGATTTGC 
CCACTGGACT ACACAACAGT GCACAACCCA GTTGTTAAAA GCCCAAAAGC CAGGTCAAGT 
CCAGGCTTTG CCAAGCCTAC TTTCTCTTAT AATATGCACA TCTCCACTGA ACTGCAGGAT 
GATGGTTACA CTCCGCTTCC TCCACACGCT CATTCAGCAC ACAGCCTGCT TGAATATTCA 
GAGGAGACAA TCACACTCTC TTCCTCTCCA GAGTCCAGCT CCATCAATGA CACAGAGCTA 
GGTTTGGTGG CGAAGGCTTC TTGGATGGAG AAGACCAGTG CTAAGAGTCA TGCAGAAGAC 
AGGGCTTCCC TGAAAAAGCA CAAAGACCGG CATAAGTTTC TGCACAACCC TCGCTTCTTG 
CTGCCAAACA CTCAGCATGG AGGAAAGTCT CTCATCATCC CAGGGAAAAA GAAGCTGGAG 
AATGTGGGAA AGAGCAGGAA AAACAACGGC AGAGAGAGAA GTCTTGAGGG TCATGCGCCT 
ATTTTCACTG CAAATCCACC AGTGATCTTC TTCAGTGACT ACAGAGTAGG ACAAGTCTAT 
GAGACCTCAG TGGAGCTCAA AAACATGACT GCGAGCAGCC GTCACATTCG AATGATTCCT 
CCAACCTCCC CACACTTCTC TGTGGGTTTG GGCCGGTTCC CTGGTGAAGG AGGCATCGTG 
GCTCCAGGAA TGAGCTGTCA GTATATGGTG CGTTTTGCTC CAGATTCTCT AGCAGACTAT 
GAAGACGCTC TGGTTGTGGA GACGCAGATG CTCTATCCTC TCATTATACC AATAGAAGCC 
TGCAGATCTC CTCCGATACT CACCTTGCCG GCTGTCATGG ACTGTGGTTT CTGCCTGGTG 
GGAGGAGTGA AGTTTATGGA GGTTTTATGT CGGAATGAAG GACTGAGTGC TGGAACGTTT 
TGTGTAATGC CTAAGAAACA GTGGCCTGCT TCAAGCCTCA GGTCTGCAGT GAAGTCGACT 
TTCACAGAAC AGCCTCCCTT TGCTATCAGT CCCTCTATTT TCGGCCTTCA CCCTGGACAG 
GCTACTGTCA TAGAGGTTGT GTTTTTCCCC ACAACTCCTG AGCTCTCTAC TCAAGACTTC 
ACCATTGTCT GTGACAACTG CCAAGTGAAG GACATTACGC TCCAAGGTAC TGGTCAACTG 
ATCACTGTGG AGCTGCTTTC AGTATCAGGT GGAGAGGATC AGCCTGAATT TGCAGAATTG 
TGTGACATCA CAGCTGACCA TTATGTCAGA TTTAACACAA CCAATCCACA CTCTACCCTG 
CAGAAGACAC TGGTTGTCAA AAACAACACA CATTTGGAGC TTCCATATCA CTGGCAGATC 
ATGAAGCCCA GTCTTCAATG CTTGTTGCCC GGAGAGACTC CTGACCCTTC CAGCATTCAG 
CATCTTCTTG ATACAGACAG TGCATTCAGC ATCAGTCCTG TTATAGGAAT CCTGAACCCT 
GCACAGGAAC ACGAGTTCCT GCTCACCTAT GCTCCCAAAG ATATAAAAGA TTACCACAGT 
GTCTGCCACT TGGTGATTAT GGATGTTCCC AGTCCACCTA AAGTCTGTGA CGATGGGACA 
TGTCCTGATA CTGCACTCCA TGCAAACAAT GTGACCATTT TAGAGGTTGA AGTGAAAGGT 
TCTACTGAGC CATACAAGAT CCTTCTTGAG CCGTATGCTG TTTTAATACC AGGAGAGACC 
TATATACACA ACACAATTTG CAAGAGATTC AAGATGTGGA ATCACAGCAA ATCAGTCATT 
CACTTTGAAT GGGAACGTGT TATTGACAGT CATGTAATTG AAGTGGAGCC CTCATCTGGA 
GTAATAGAAA TGAATGAGTG TTTTGACATG GAGTTGTATC TGACTGGAAG GAGTCCGGGT 
CATTTTACCT CTACCCTGCA GTGTCACATT CTGCACCATC CTAAAACTAT AGGACTTGCC 
ATTGAGGCCA CATTTAAGGG TCCACATTGC TCATTGAATG TACCCAGTCT GGATTTTGGT 
TTGCTGCAAA TGGGAAATGA GAGAATTTTG ACTATTGAAG TCACCAACAA CAGCCTGCTA 
GATGCCCACT GGAGCCTGGA GGTGCTGTCC CAAACCCAGA AGCTCATCGA TGGACAGGTG 
CATATAAATC CAAATAAAGG TGTTCTGGCT GCACTAGAGA GCTGCAGTGT GGATGTGGCC 
TTCAGAGCCA TGCATTGCCA AGTATTTGAG TCAGTTTTAC AACTTTCTGT GCTGAACGGC 
ACCGGTTGTC ACGTCTCCAT CCATGCCGTT GTTCAGTCTC CTCAAGTGTG CTTGCTGAAC 
TGTGAGTTGC TGTTTGCTGA TCTGTATGTT GGGGTTTCAC AGACGGGCAG TGTCACTCTG 
TTTAACCAAA CACTACTGCC AGCTCACTTC ACCTGGGGCA GGCTTCAAGG TTCACAAGCT 
CATCTCTGCT CCGCAGTCTT CAGTCCCTCT GCAGGATCTC TGGGGCCCAA TGCAAAAACT 
GAAGTCTCTG TGTCATTCAC AGCTCACACT GTTGAGGATT TGACTGAAGT GAATGGAGTT 
TGTGAGGTAA AAGGAATGGA GAATCCACTT GTGCTGGGAA TTTTTTCTAA AGCCAAACCT 
CTCAGAGTTT CCTACTCACT GCTTTGTAAT GGTATTAATT CAGGTGATGG CGATCACCAA 
CCAATCCTGC TGGACTTCAC TGAGCATGAG CCAGCCGTCA TTGGGAAATC TGTCAAACGG 
CAGTTACTGA TAACTAACAA CACTGCTATT ACTGCTCCAT TCACTGTCGA CACTGAGTAC 
TTCACTGGAG ACTGTCCTTC ACAATCAGAA GGAAACTCTG AAAGAAGCAG TGCACCTTTC 
CACTCCATCC AGGCCAAAAA AATACAGCAA AAGGCATATG ATGAGTTTGT AAGCCGTCTG 
CTTTCCCATG GAAGAGGTGC AACATTTTAT GTAGAACCAA AAAGTGGGAT GCTTGGCCCA 
TTCGAGAGTC AAACCATCAA CATCACTACT TTCACAAATA TGTGGGGGGA TTATCAGGAC 
AACCTCATAT GCAAAGTTGG AGATCTAAAT CCAACTCACA TTCATATGAA GATGTCTGTG 
AGGGGCTGTC CAGTTTACTT TCAGATGATT GGACCACAGC CTGACAATCA GAATCAGGGT 
CCAGTTATTA GATTTGGAAG CCATGTTTCT GGAGGAGACA CTGTGTCACG CTCTTTACGA 
CTCATTAACA CCAGTCCATA TGATATCCGT ATGGACTGGG TGACCTACAA CCTGGAGGCT 
GGAGACGGCA AGCTGATAGA TCTGCTGCTT GCATGTGGTG AACCTTTCCC TCTGAAGGAT 
GCAGATGGCA ATGAGGTTGT TGGAGGACTG GACTCATCTG TCATGTTCCC GTCCACATGG 
GATGACAGTC ACACCCCCAG CAGAGAAGGA ACAAGCTCAA CTTTTATGAC CCAATCTGAT 
GATTTTGTAG AGAATGGTGA AGAATGTGAT GGAGAAGATG GCAGTGGGGC TTCTGAGTCT 
CTAGCTCCAG TGAAGAAGCT GTTTTCTGTG TTTTTGAAGC CTCATGAAGG GACTCTTTCT 
GACTACCCAT ACTGCATCAC TCCACAGCAG ACTGTAGTTC CAGCAGGGGG CAGTAGCACC 
ATTCATTTGT CTTTCACTCC CCTGATTCTG TCATGTTCAA CCAATCAAAG ATGTTTGGGT 
TACGCTCTTG GTTTCATGAG CTTAGACTCT AAGCTTGCGT TAGTGACTCC AGGCAAGGTG 
GAGAGAGTCC GGGGCTATGA ACTTCAACCT TTGAGGCTGG ACATGCAGGC TAATGTAAAA 
CCTGCCATTT TGTCAGTGCA GATGGCAGAG GATACAGAAG TGCTGGAATT CAGCGCAGCT 
GCCAGCGACA CACTAGACGG AGCATCACAA ACACAGGAAT CCAGGATAAC TCGCACATTC 
CGGCTAAAGA ACGACACAGA CATGGCCTTG AGTTTCAAAC TGAGCACAAA TCCACCATTT 
TCAGTGCTGC TGCCCCGACA GAGCCTTAAA CTGATCAGCA GCTCGCCTGC TTATAGACAC 
ACACAAGGTC TGTCCGGACC AGGAGATGAG ATGAGATTAT TTCTGTTACA GCCCAAAAAC 
ATCATTCAGG TTAAGGTGGC TTTCCACAAT TCCGCTTCAC TCCTGGCCTG TGTAAATGGA 
GCATGTGAGG AGTCGGACAG TCAGCTCTCT GCTTCCCTTC TTTGCAGTGA TGATGGAGGA 
AAAACTCTAC AGTTTCAGGA GAACTTGACC ATTCAGTACA GCAATAACAG CATACAGACA 
GTCTCTCTTT ACGCTCGTCT GGCATTACCT ACACTACACC TGTCCAGTGC CACTGTGGAT 
TTTGGCACCT GTTATGTTGG ACAAACAACT GTCAAAGAGA TTTATCTTCA CAACAGAGGT 
GGCTCCTGTA GCTACTGGAC CACACTCACA GATGGCAATG AAGTGTTCAG GGTAAGTCCG 
GATTCTGGTT TACTGAAACC TCTTGGAGAT CCAGCGTCCT GTAGACAGCT GCTGCAGATC 
AGCTTCACTC CGAGAGAACA GAAAGAGTTC CAGACAATCT TCACAGTGCA GGGGCTTCTT 
GGAGAGACAC CACTTGTTCT AAAGGTCAGC GGAAGAGGAT CATTTGATGA GAGTTTTGTA 
TCTCCAATAA CTGACAGCTC ACAAAACAAC ACTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlec1 ( XM_678134.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_683226.4 Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X1, mRNA.

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CloneID	ODa28564
Clone ID Related Accession (Same CDS sequence)	XM_678134.6 , XM_678134.7
Accession Version	XM_678134.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	deleted in lung and esophageal cancer protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_678134.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATG AAACTGAACC AAAAGCACTA ACGTTAAAGA GTTCAGAACC GTCAATAAAC 
CGGCACACAC CAGCTTCAAA GACAACTCAG GACATTTCTC ATCTGCTGGC CAGCATATTC 
AATGATCTCT ACAGCACAGA GGTGATTGGG AAGGACACCG TTTCCAATCT CAGTAAATCA 
CGAAGAAGAG GCAACAGCTA CCATGAAAAA TATGTTGAGG AGCTACAACA GGTGCACAGT 
GAATACAACA GACGTATTCA GAATGCTGAT ATGCTAGAGA CACACATCAT ACAGGCCCGT 
CTTCAGGCTG CTGCAAAAGA AGAGCATGAT CGCAACAAAA TCCTGGAAGA AGTCGGTGAG 
GCTTACCAAC AGCTGGGACT TCCCCCAGTA AAATCAGCTT TCAAGTGGTG TGTGGACAGT 
GGACTTTTGA GGAGCAACAG CTTGATTTGC CCACTGGACT ACACAACAGT GCACAACCCA 
GTTGTTAAAA GCCCAAAAGC CAGGTCAAGT CCAGGCTTTG CCAAGCCTAC TTTCTCTTAT 
AATATGCACA TCTCCACTGA ACTGCAGGAT GATGGTTACA CTCCGCTTCC TCCACACGCT 
CATTCAGCAC ACAGCCTGCT TGAATATTCA GAGGAGACAA TCACACTCTC TTCCTCTCCA 
GAGTCCAGCT CCATCAATGA CACAGAGCTA GGTTTGGTGG CGAAGGCTTC TTGGATGGAG 
AAGACCAGTG CTAAGAGTCA TGCAGAAGAC AGGGCTTCCC TGAAAAAGCA CAAAGACCGG 
CATAAGTTTC TGCACAACCC TCGCTTCTTG CTGCCAAACA CTCAGCATGG AGGAAAGTCT 
CTCATCATCC CAGGGAAAAA GAAGCTGGAG AATGTGGGAA AGAGCAGGAA AAACAACGGC 
AGAGAGAGAA GTCTTGAGGG TCATGCGCCT ATTTTCACTG CAAATCCACC AGTGATCTTC 
TTCAGTGACT ACAGAGTAGG ACAAGTCTAT GAGACCTCAG TGGAGCTCAA AAACATGACT 
GCGAGCAGCC GTCACATTCG AATGATTCCT CCAACCTCCC CACACTTCTC TGTGGGTTTG 
GGCCGGTTCC CTGGTGAAGG AGGCATCGTG GCTCCAGGAA TGAGCTGTCA GTATATGGTG 
CGTTTTGCTC CAGATTCTCT AGCAGACTAT GAAGACGCTC TGGTTGTGGA GACGCAGATG 
CTCTATCCTC TCATTATACC AATAGAAGCC TGCAGATCTC CTCCGATACT CACCTTGCCG 
GCTGTCATGG ACTGTGGTTT CTGCCTGGTG GGAGGAGTGA AGTTTATGGA GGTTTTATGT 
CGGAATGAAG GACTGAGTGC TGGAACGTTT TGTGTAATGC CTAAGAAACA GTGGCCTGCT 
TCAAGCCTCA GGTCTGCAGT GAAGTCGACT TTCACAGAAC AGCCTCCCTT TGCTATCAGT 
CCCTCTATTT TCGGCCTTCA CCCTGGACAG GCTACTGTCA TAGAGGTTGT GTTTTTCCCC 
ACAACTCCTG AGCTCTCTAC TCAAGACTTC ACCATTGTCT GTGACAACTG CCAAGTGAAG 
GACATTACGC TCCAAGGTAC TGGTCAACTG ATCACTGTGG AGCTGCTTTC AGTATCAGGT 
GGAGAGGATC AGCCTGAATT TGCAGAATTG TGTGACATCA CAGCTGACCA TTATGTCAGA 
TTTAACACAA CCAATCCACA CTCTACCCTG CAGAAGACAC TGGTTGTCAA AAACAACACA 
CATTTGGAGC TTCCATATCA CTGGCAGATC ATGAAGCCCA GTCTTCAATG CTTGTTGCCC 
GGAGAGACTC CTGACCCTTC CAGCATTCAG CATCTTCTTG ATACAGACAG TGCATTCAGC 
ATCAGTCCTG TTATAGGAAT CCTGAACCCT GCACAGGAAC ACGAGTTCCT GCTCACCTAT 
GCTCCCAAAG ATATAAAAGA TTACCACAGT GTCTGCCACT TGGTGATTAT GGATGTTCCC 
AGTCCACCTA AAGTCTGTGA CGATGGGACA TGTCCTGATA CTGCACTCCA TGCAAACAAT 
GTGACCATTT TAGAGGTTGA AGTGAAAGGT TCTACTGAGC CATACAAGAT CCTTCTTGAG 
CCGTATGCTG TTTTAATACC AGGAGAGACC TATATACACA ACACAATTTG CAAGAGATTC 
AAGATGTGGA ATCACAGCAA ATCAGTCATT CACTTTGAAT GGGAACGTGT TATTGACAGT 
CATGTAATTG AAGTGGAGCC CTCATCTGGA GTAATAGAAA TGAATGAGTG TTTTGACATG 
GAGTTGTATC TGACTGGAAG GAGTCCGGGT CATTTTACCT CTACCCTGCA GTGTCACATT 
CTGCACCATC CTAAAACTAT AGGACTTGCC ATTGAGGCCA CATTTAAGGG TCCACATTGC 
TCATTGAATG TACCCAGTCT GGATTTTGGT TTGCTGCAAA TGGGAAATGA GAGAATTTTG 
ACTATTGAAG TCACCAACAA CAGCCTGCTA GATGCCCACT GGAGCCTGGA GGTGCTGTCC 
CAAACCCAGA AGCTCATCGA TGGACAGGTG CATATAAATC CAAATAAAGG TGTTCTGGCT 
GCACTAGAGA GCTGCAGTGT GGATGTGGCC TTCAGAGCCA TGCATTGCCA AGTATTTGAG 
TCAGTTTTAC AACTTTCTGT GCTGAACGGC ACCGGTTGTC ACGTCTCCAT CCATGCCGTT 
GTTCAGTCTC CTCAAGTGTG CTTGCTGAAC TGTGAGTTGC TGTTTGCTGA TCTGTATGTT 
GGGGTTTCAC AGACGGGCAG TGTCACTCTG TTTAACCAAA CACTACTGCC AGCTCACTTC 
ACCTGGGGCA GGCTTCAAGG TTCACAAGCT CATCTCTGCT CCGCAGTCTT CAGTCCCTCT 
GCAGGATCTC TGGGGCCCAA TGCAAAAACT GAAGTCTCTG TGTCATTCAC AGCTCACACT 
GTTGAGGATT TGACTGAAGT GAATGGAGTT TGTGAGGTAA AAGGAATGGA GAATCCACTT 
GTGCTGGGAA TTTTTTCTAA AGCCAAACCT CTCAGAGTTT CCTACTCACT GCTTTGTAAT 
GGTATTAATT CAGGTGATGG CGATCACCAA CCAATCCTGC TGGACTTCAC TGAGCATGAG 
CCAGCCGTCA TTGGGAAATC TGTCAAACGG CAGTTACTGA TAACTAACAA CACTGCTATT 
ACTGCTCCAT TCACTGTCGA CACTGAGTAC TTCACTGGAG ACTGTCCTTC ACAATCAGAA 
GGAAACTCTG AAAGAAGCAG TGCACCTTTC CACTCCATCC AGGCCAAAAA AATACAGCAA 
AAGGCATATG ATGAGTTTGT AAGCCGTCTG CTTTCCCATG GAAGAGGTGC AACATTTTAT 
GTAGAACCAA AAAGTGGGAT GCTTGGCCCA TTCGAGAGTC AAACCATCAA CATCACTACT 
TTCACAAATA TGTGGGGGGA TTATCAGGAC AACCTCATAT GCAAAGTTGG AGATCTAAAT 
CCAACTCACA TTCATATGAA GATGTCTGTG AGGGGCTGTC CAGTTTACTT TCAGATGATT 
GGACCACAGC CTGACAATCA GAATCAGGGT CCAGTTATTA GATTTGGAAG CCATGTTTCT 
GGAGGAGACA CTGTGTCACG CTCTTTACGA CTCATTAACA CCAGTCCATA TGATATCCGT 
ATGGACTGGG TGACCTACAA CCTGGAGGCT GGAGACGGCA AGCTGATAGA TCTGCTGCTT 
GCATGTGGTG AACCTTTCCC TCTGAAGGAT GCAGATGGCA ATGAGGTTGT TGGAGGACTG 
GACTCATCTG TCATGTTCCC GTCCACATGG GATGACAGTC ACACCCCCAG CAGAGAAGGA 
ACAAGCTCAA CTTTTATGAC CCAATCTGAT GATTTTGTAG AGAATGGTGA AGAATGTGAT 
GGAGAAGATG GCAGTGGGGC TTCTGAGTCT CTAGCTCCAG TGAAGAAGCT GTTTTCTGTG 
TTTTTGAAGC CTCATGAAGG GACTCTTTCT GACTACCCAT ACTGCATCAC TCCACAGCAG 
ACTGTAGTTC CAGCAGGGGG CAGTAGCACC ATTCATTTGT CTTTCACTCC CCTGATTCTG 
TCATGTTCAA CCAATCAAAG ATGTTTGGGT TACGCTCTTG GTTTCATGAG CTTAGACTCT 
AAGCTTGCGT TAGTGACTCC AGGCAAGGTG GAGAGAGTCC GGGGCTATGA ACTTCAACCT 
TTGAGGCTGG ACATGCAGGC TAATGTAAAA CCTGCCATTT TGTCAGTGCA GATGGCAGAG 
GATACAGAAG TGCTGGAATT CAGCGCAGCT GCCAGCGACA CACTAGACGG AGCATCACAA 
ACACAGGAAT CCAGGATAAC TCGCACATTC CGGCTAAAGA ACGACACAGA CATGGCCTTG 
AGTTTCAAAC TGAGCACAAA TCCACCATTT TCAGTGCTGC TGCCCCGACA GAGCCTTAAA 
CTGATCAGCA GCTCGCCTGC TTATAGACAC ACACAAGGTC TGTCCGGACC AGGAGATGAG 
ATGAGATTAT TTCTGTTACA GCCCAAAAAC ATCATTCAGG TTAAGGTGGC TTTCCACAAT 
TCCGCTTCAC TCCTGGCCTG TGTAAATGGA GCATGTGAGG AGTCGGACAG TCAGCTCTCT 
GCTTCCCTTC TTTGCAGTGA TGATGGAGGA AAAACTCTAC AGTTTCAGGA GAACTTGACC 
ATTCAGTACA GCAATAACAG CATACAGACA GTCTCTCTTT ACGCTCGTCT GGCATTACCT 
ACACTACACC TGTCCAGTGC CACTGTGGAT TTTGGCACCT GTTATGTTGG ACAAACAACT 
GTCAAAGAGA TTTATCTTCA CAACAGAGGT GGCTCCTGTA GCTACTGGAC CACACTCACA 
GATGGCAATG AAGTGTTCAG GGTAAGTCCG GATTCTGGTT TACTGAAACC TCTTGGAGAT 
CCAGCGTCCT GTAGACAGCT GCTGCAGATC AGCTTCACTC CGAGAGAACA GAAAGAGTTC 
CAGACAATCT TCACAGTGCA GGGGCTTCTT GGAGAGACAC CACTTGTTCT AAAGGTCAGC 
GGAAGAGGAT CATTTGATGA GAGTTTTGTA TCTCCAATAA CTGACAGCTC ACAAAACAAC 
ACTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_683226.4
CDS	180..5288
Translation	MADETEPKALTLKSSEPSINRHTPASKTTQDISHLLASIFNDLYSTEVIGKDTVSNLSKSRRRGNSYHEKYVEELQQVHSEYNRRIQNADMLETHIIQARLQAAAKEEHDRNKILEEVGEAYQQLGLPPVKSAFKWCVDSGLLRSNSLICPLDYTTVHNPVVKSPKARSSPGFAKPTFSYNMHISTELQDDGYTPLPPHAHSAHSLLEYSEETITLSSSPESSSINDTELGLVAKASWMEKTSAKSHAEDRASLKKHKDRHKFLHNPRFLLPNTQHGGKSLIIPGKKKLENVGKSRKNNGRERSLEGHAPIFTANPPVIFFSDYRVGQVYETSVELKNMTASSRHIRMIPPTSPHFSVGLGRFPGEGGIVAPGMSCQYMVRFAPDSLADYEDALVVETQMLYPLIIPIEACRSPPILTLPAVMDCGFCLVGGVKFMEVLCRNEGLSAGTFCVMPKKQWPASSLRSAVKSTFTEQPPFAISPSIFGLHPGQATVIEVVFFPTTPELSTQDFTIVCDNCQVKDITLQGTGQLITVELLSVSGGEDQPEFAELCDITADHYVRFNTTNPHSTLQKTLVVKNNTHLELPYHWQIMKPSLQCLLPGETPDPSSIQHLLDTDSAFSISPVIGILNPAQEHEFLLTYAPKDIKDYHSVCHLVIMDVPSPPKVCDDGTCPDTALHANNVTILEVEVKGSTEPYKILLEPYAVLIPGETYIHNTICKRFKMWNHSKSVIHFEWERVIDSHVIEVEPSSGVIEMNECFDMELYLTGRSPGHFTSTLQCHILHHPKTIGLAIEATFKGPHCSLNVPSLDFGLLQMGNERILTIEVTNNSLLDAHWSLEVLSQTQKLIDGQVHINPNKGVLAALESCSVDVAFRAMHCQVFESVLQLSVLNGTGCHVSIHAVVQSPQVCLLNCELLFADLYVGVSQTGSVTLFNQTLLPAHFTWGRLQGSQAHLCSAVFSPSAGSLGPNAKTEVSVSFTAHTVEDLTEVNGVCEVKGMENPLVLGIFSKAKPLRVSYSLLCNGINSGDGDHQPILLDFTEHEPAVIGKSVKRQLLITNNTAITAPFTVDTEYFTGDCPSQSEGNSERSSAPFHSIQAKKIQQKAYDEFVSRLLSHGRGATFYVEPKSGMLGPFESQTINITTFTNMWGDYQDNLICKVGDLNPTHIHMKMSVRGCPVYFQMIGPQPDNQNQGPVIRFGSHVSGGDTVSRSLRLINTSPYDIRMDWVTYNLEAGDGKLIDLLLACGEPFPLKDADGNEVVGGLDSSVMFPSTWDDSHTPSREGTSSTFMTQSDDFVENGEECDGEDGSGASESLAPVKKLFSVFLKPHEGTLSDYPYCITPQQTVVPAGGSSTIHLSFTPLILSCSTNQRCLGYALGFMSLDSKLALVTPGKVERVRGYELQPLRLDMQANVKPAILSVQMAEDTEVLEFSAAASDTLDGASQTQESRITRTFRLKNDTDMALSFKLSTNPPFSVLLPRQSLKLISSSPAYRHTQGLSGPGDEMRLFLLQPKNIIQVKVAFHNSASLLACVNGACEESDSQLSASLLCSDDGGKTLQFQENLTIQYSNNSIQTVSLYARLALPTLHLSSATVDFGTCYVGQTTVKEIYLHNRGGSCSYWTTLTDGNEVFRVSPDSGLLKPLGDPASCRQLLQISFTPREQKEFQTIFTVQGLLGETPLVLKVSGRGSFDESFVSPITDSSQNNTE

Target ORF information:

RefSeq Version	XM_678134.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_678134.6

ORF Insert Sequence:

ATGGCTGATG AAACTGAACC AAAAGCACTA ACGTTAAAGA GTTCAGAACC GTCAATAAAC 
CGGCACACAC CAGCTTCAAA GACAACTCAG GACATTTCTC ATCTGCTGGC CAGCATATTC 
AATGATCTCT ACAGCACAGA GGTGATTGGG AAGGACACCG TTTCCAATCT CAGTAAATCA 
CGAAGAAGAG GCAACAGCTA CCATGAAAAA TATGTTGAGG AGCTACAACA GGTGCACAGT 
GAATACAACA GACGTATTCA GAATGCTGAT ATGCTAGAGA CACACATCAT ACAGGCCCGT 
CTTCAGGCTG CTGCAAAAGA AGAGCATGAT CGCAACAAAA TCCTGGAAGA AGTCGGTGAG 
GCTTACCAAC AGCTGGGACT TCCCCCAGTA AAATCAGCTT TCAAGTGGTG TGTGGACAGT 
GGACTTTTGA GGAGCAACAG CTTGATTTGC CCACTGGACT ACACAACAGT GCACAACCCA 
GTTGTTAAAA GCCCAAAAGC CAGGTCAAGT CCAGGCTTTG CCAAGCCTAC TTTCTCTTAT 
AATATGCACA TCTCCACTGA ACTGCAGGAT GATGGTTACA CTCCGCTTCC TCCACACGCT 
CATTCAGCAC ACAGCCTGCT TGAATATTCA GAGGAGACAA TCACACTCTC TTCCTCTCCA 
GAGTCCAGCT CCATCAATGA CACAGAGCTA GGTTTGGTGG CGAAGGCTTC TTGGATGGAG 
AAGACCAGTG CTAAGAGTCA TGCAGAAGAC AGGGCTTCCC TGAAAAAGCA CAAAGACCGG 
CATAAGTTTC TGCACAACCC TCGCTTCTTG CTGCCAAACA CTCAGCATGG AGGAAAGTCT 
CTCATCATCC CAGGGAAAAA GAAGCTGGAG AATGTGGGAA AGAGCAGGAA AAACAACGGC 
AGAGAGAGAA GTCTTGAGGG TCATGCGCCT ATTTTCACTG CAAATCCACC AGTGATCTTC 
TTCAGTGACT ACAGAGTAGG ACAAGTCTAT GAGACCTCAG TGGAGCTCAA AAACATGACT 
GCGAGCAGCC GTCACATTCG AATGATTCCT CCAACCTCCC CACACTTCTC TGTGGGTTTG 
GGCCGGTTCC CTGGTGAAGG AGGCATCGTG GCTCCAGGAA TGAGCTGTCA GTATATGGTG 
CGTTTTGCTC CAGATTCTCT AGCAGACTAT GAAGACGCTC TGGTTGTGGA GACGCAGATG 
CTCTATCCTC TCATTATACC AATAGAAGCC TGCAGATCTC CTCCGATACT CACCTTGCCG 
GCTGTCATGG ACTGTGGTTT CTGCCTGGTG GGAGGAGTGA AGTTTATGGA GGTTTTATGT 
CGGAATGAAG GACTGAGTGC TGGAACGTTT TGTGTAATGC CTAAGAAACA GTGGCCTGCT 
TCAAGCCTCA GGTCTGCAGT GAAGTCGACT TTCACAGAAC AGCCTCCCTT TGCTATCAGT 
CCCTCTATTT TCGGCCTTCA CCCTGGACAG GCTACTGTCA TAGAGGTTGT GTTTTTCCCC 
ACAACTCCTG AGCTCTCTAC TCAAGACTTC ACCATTGTCT GTGACAACTG CCAAGTGAAG 
GACATTACGC TCCAAGGTAC TGGTCAACTG ATCACTGTGG AGCTGCTTTC AGTATCAGGT 
GGAGAGGATC AGCCTGAATT TGCAGAATTG TGTGACATCA CAGCTGACCA TTATGTCAGA 
TTTAACACAA CCAATCCACA CTCTACCCTG CAGAAGACAC TGGTTGTCAA AAACAACACA 
CATTTGGAGC TTCCATATCA CTGGCAGATC ATGAAGCCCA GTCTTCAATG CTTGTTGCCC 
GGAGAGACTC CTGACCCTTC CAGCATTCAG CATCTTCTTG ATACAGACAG TGCATTCAGC 
ATCAGTCCTG TTATAGGAAT CCTGAACCCT GCACAGGAAC ACGAGTTCCT GCTCACCTAT 
GCTCCCAAAG ATATAAAAGA TTACCACAGT GTCTGCCACT TGGTGATTAT GGATGTTCCC 
AGTCCACCTA AAGTCTGTGA CGATGGGACA TGTCCTGATA CTGCACTCCA TGCAAACAAT 
GTGACCATTT TAGAGGTTGA AGTGAAAGGT TCTACTGAGC CATACAAGAT CCTTCTTGAG 
CCGTATGCTG TTTTAATACC AGGAGAGACC TATATACACA ACACAATTTG CAAGAGATTC 
AAGATGTGGA ATCACAGCAA ATCAGTCATT CACTTTGAAT GGGAACGTGT TATTGACAGT 
CATGTAATTG AAGTGGAGCC CTCATCTGGA GTAATAGAAA TGAATGAGTG TTTTGACATG 
GAGTTGTATC TGACTGGAAG GAGTCCGGGT CATTTTACCT CTACCCTGCA GTGTCACATT 
CTGCACCATC CTAAAACTAT AGGACTTGCC ATTGAGGCCA CATTTAAGGG TCCACATTGC 
TCATTGAATG TACCCAGTCT GGATTTTGGT TTGCTGCAAA TGGGAAATGA GAGAATTTTG 
ACTATTGAAG TCACCAACAA CAGCCTGCTA GATGCCCACT GGAGCCTGGA GGTGCTGTCC 
CAAACCCAGA AGCTCATCGA TGGACAGGTG CATATAAATC CAAATAAAGG TGTTCTGGCT 
GCACTAGAGA GCTGCAGTGT GGATGTGGCC TTCAGAGCCA TGCATTGCCA AGTATTTGAG 
TCAGTTTTAC AACTTTCTGT GCTGAACGGC ACCGGTTGTC ACGTCTCCAT CCATGCCGTT 
GTTCAGTCTC CTCAAGTGTG CTTGCTGAAC TGTGAGTTGC TGTTTGCTGA TCTGTATGTT 
GGGGTTTCAC AGACGGGCAG TGTCACTCTG TTTAACCAAA CACTACTGCC AGCTCACTTC 
ACCTGGGGCA GGCTTCAAGG TTCACAAGCT CATCTCTGCT CCGCAGTCTT CAGTCCCTCT 
GCAGGATCTC TGGGGCCCAA TGCAAAAACT GAAGTCTCTG TGTCATTCAC AGCTCACACT 
GTTGAGGATT TGACTGAAGT GAATGGAGTT TGTGAGGTAA AAGGAATGGA GAATCCACTT 
GTGCTGGGAA TTTTTTCTAA AGCCAAACCT CTCAGAGTTT CCTACTCACT GCTTTGTAAT 
GGTATTAATT CAGGTGATGG CGATCACCAA CCAATCCTGC TGGACTTCAC TGAGCATGAG 
CCAGCCGTCA TTGGGAAATC TGTCAAACGG CAGTTACTGA TAACTAACAA CACTGCTATT 
ACTGCTCCAT TCACTGTCGA CACTGAGTAC TTCACTGGAG ACTGTCCTTC ACAATCAGAA 
GGAAACTCTG AAAGAAGCAG TGCACCTTTC CACTCCATCC AGGCCAAAAA AATACAGCAA 
AAGGCATATG ATGAGTTTGT AAGCCGTCTG CTTTCCCATG GAAGAGGTGC AACATTTTAT 
GTAGAACCAA AAAGTGGGAT GCTTGGCCCA TTCGAGAGTC AAACCATCAA CATCACTACT 
TTCACAAATA TGTGGGGGGA TTATCAGGAC AACCTCATAT GCAAAGTTGG AGATCTAAAT 
CCAACTCACA TTCATATGAA GATGTCTGTG AGGGGCTGTC CAGTTTACTT TCAGATGATT 
GGACCACAGC CTGACAATCA GAATCAGGGT CCAGTTATTA GATTTGGAAG CCATGTTTCT 
GGAGGAGACA CTGTGTCACG CTCTTTACGA CTCATTAACA CCAGTCCATA TGATATCCGT 
ATGGACTGGG TGACCTACAA CCTGGAGGCT GGAGACGGCA AGCTGATAGA TCTGCTGCTT 
GCATGTGGTG AACCTTTCCC TCTGAAGGAT GCAGATGGCA ATGAGGTTGT TGGAGGACTG 
GACTCATCTG TCATGTTCCC GTCCACATGG GATGACAGTC ACACCCCCAG CAGAGAAGGA 
ACAAGCTCAA CTTTTATGAC CCAATCTGAT GATTTTGTAG AGAATGGTGA AGAATGTGAT 
GGAGAAGATG GCAGTGGGGC TTCTGAGTCT CTAGCTCCAG TGAAGAAGCT GTTTTCTGTG 
TTTTTGAAGC CTCATGAAGG GACTCTTTCT GACTACCCAT ACTGCATCAC TCCACAGCAG 
ACTGTAGTTC CAGCAGGGGG CAGTAGCACC ATTCATTTGT CTTTCACTCC CCTGATTCTG 
TCATGTTCAA CCAATCAAAG ATGTTTGGGT TACGCTCTTG GTTTCATGAG CTTAGACTCT 
AAGCTTGCGT TAGTGACTCC AGGCAAGGTG GAGAGAGTCC GGGGCTATGA ACTTCAACCT 
TTGAGGCTGG ACATGCAGGC TAATGTAAAA CCTGCCATTT TGTCAGTGCA GATGGCAGAG 
GATACAGAAG TGCTGGAATT CAGCGCAGCT GCCAGCGACA CACTAGACGG AGCATCACAA 
ACACAGGAAT CCAGGATAAC TCGCACATTC CGGCTAAAGA ACGACACAGA CATGGCCTTG 
AGTTTCAAAC TGAGCACAAA TCCACCATTT TCAGTGCTGC TGCCCCGACA GAGCCTTAAA 
CTGATCAGCA GCTCGCCTGC TTATAGACAC ACACAAGGTC TGTCCGGACC AGGAGATGAG 
ATGAGATTAT TTCTGTTACA GCCCAAAAAC ATCATTCAGG TTAAGGTGGC TTTCCACAAT 
TCCGCTTCAC TCCTGGCCTG TGTAAATGGA GCATGTGAGG AGTCGGACAG TCAGCTCTCT 
GCTTCCCTTC TTTGCAGTGA TGATGGAGGA AAAACTCTAC AGTTTCAGGA GAACTTGACC 
ATTCAGTACA GCAATAACAG CATACAGACA GTCTCTCTTT ACGCTCGTCT GGCATTACCT 
ACACTACACC TGTCCAGTGC CACTGTGGAT TTTGGCACCT GTTATGTTGG ACAAACAACT 
GTCAAAGAGA TTTATCTTCA CAACAGAGGT GGCTCCTGTA GCTACTGGAC CACACTCACA 
GATGGCAATG AAGTGTTCAG GGTAAGTCCG GATTCTGGTT TACTGAAACC TCTTGGAGAT 
CCAGCGTCCT GTAGACAGCT GCTGCAGATC AGCTTCACTC CGAGAGAACA GAAAGAGTTC 
CAGACAATCT TCACAGTGCA GGGGCTTCTT GGAGAGACAC CACTTGTTCT AAAGGTCAGC 
GGAAGAGGAT CATTTGATGA GAGTTTTGTA TCTCCAATAA CTGACAGCTC ACAAAACAAC 
ACTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dlec1 ( XM_678134.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_683226.4 Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X1, mRNA.

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CloneID	ODa28564
Clone ID Related Accession (Same CDS sequence)	XM_678134.6 , XM_678134.7
Accession Version	XM_678134.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	deleted in lung and esophageal cancer protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_678134.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGATG AAACTGAACC AAAAGCACTA ACGTTAAAGA GTTCAGAACC GTCAATAAAC 
CGGCACACAC CAGCTTCAAA GACAACTCAG GACATTTCTC ATCTGCTGGC CAGCATATTC 
AATGATCTCT ACAGCACAGA GGTGATTGGG AAGGACACCG TTTCCAATCT CAGTAAATCA 
CGAAGAAGAG GCAACAGCTA CCATGAAAAA TATGTTGAGG AGCTACAACA GGTGCACAGT 
GAATACAACA GACGTATTCA GAATGCTGAT ATGCTAGAGA CACACATCAT ACAGGCCCGT 
CTTCAGGCTG CTGCAAAAGA AGAGCATGAT CGCAACAAAA TCCTGGAAGA AGTCGGTGAG 
GCTTACCAAC AGCTGGGACT TCCCCCAGTA AAATCAGCTT TCAAGTGGTG TGTGGACAGT 
GGACTTTTGA GGAGCAACAG CTTGATTTGC CCACTGGACT ACACAACAGT GCACAACCCA 
GTTGTTAAAA GCCCAAAAGC CAGGTCAAGT CCAGGCTTTG CCAAGCCTAC TTTCTCTTAT 
AATATGCACA TCTCCACTGA ACTGCAGGAT GATGGTTACA CTCCGCTTCC TCCACACGCT 
CATTCAGCAC ACAGCCTGCT TGAATATTCA GAGGAGACAA TCACACTCTC TTCCTCTCCA 
GAGTCCAGCT CCATCAATGA CACAGAGCTA GGTTTGGTGG CGAAGGCTTC TTGGATGGAG 
AAGACCAGTG CTAAGAGTCA TGCAGAAGAC AGGGCTTCCC TGAAAAAGCA CAAAGACCGG 
CATAAGTTTC TGCACAACCC TCGCTTCTTG CTGCCAAACA CTCAGCATGG AGGAAAGTCT 
CTCATCATCC CAGGGAAAAA GAAGCTGGAG AATGTGGGAA AGAGCAGGAA AAACAACGGC 
AGAGAGAGAA GTCTTGAGGG TCATGCGCCT ATTTTCACTG CAAATCCACC AGTGATCTTC 
TTCAGTGACT ACAGAGTAGG ACAAGTCTAT GAGACCTCAG TGGAGCTCAA AAACATGACT 
GCGAGCAGCC GTCACATTCG AATGATTCCT CCAACCTCCC CACACTTCTC TGTGGGTTTG 
GGCCGGTTCC CTGGTGAAGG AGGCATCGTG GCTCCAGGAA TGAGCTGTCA GTATATGGTG 
CGTTTTGCTC CAGATTCTCT AGCAGACTAT GAAGACGCTC TGGTTGTGGA GACGCAGATG 
CTCTATCCTC TCATTATACC AATAGAAGCC TGCAGATCTC CTCCGATACT CACCTTGCCG 
GCTGTCATGG ACTGTGGTTT CTGCCTGGTG GGAGGAGTGA AGTTTATGGA GGTTTTATGT 
CGGAATGAAG GACTGAGTGC TGGAACGTTT TGTGTAATGC CTAAGAAACA GTGGCCTGCT 
TCAAGCCTCA GGTCTGCAGT GAAGTCGACT TTCACAGAAC AGCCTCCCTT TGCTATCAGT 
CCCTCTATTT TCGGCCTTCA CCCTGGACAG GCTACTGTCA TAGAGGTTGT GTTTTTCCCC 
ACAACTCCTG AGCTCTCTAC TCAAGACTTC ACCATTGTCT GTGACAACTG CCAAGTGAAG 
GACATTACGC TCCAAGGTAC TGGTCAACTG ATCACTGTGG AGCTGCTTTC AGTATCAGGT 
GGAGAGGATC AGCCTGAATT TGCAGAATTG TGTGACATCA CAGCTGACCA TTATGTCAGA 
TTTAACACAA CCAATCCACA CTCTACCCTG CAGAAGACAC TGGTTGTCAA AAACAACACA 
CATTTGGAGC TTCCATATCA CTGGCAGATC ATGAAGCCCA GTCTTCAATG CTTGTTGCCC 
GGAGAGACTC CTGACCCTTC CAGCATTCAG CATCTTCTTG ATACAGACAG TGCATTCAGC 
ATCAGTCCTG TTATAGGAAT CCTGAACCCT GCACAGGAAC ACGAGTTCCT GCTCACCTAT 
GCTCCCAAAG ATATAAAAGA TTACCACAGT GTCTGCCACT TGGTGATTAT GGATGTTCCC 
AGTCCACCTA AAGTCTGTGA CGATGGGACA TGTCCTGATA CTGCACTCCA TGCAAACAAT 
GTGACCATTT TAGAGGTTGA AGTGAAAGGT TCTACTGAGC CATACAAGAT CCTTCTTGAG 
CCGTATGCTG TTTTAATACC AGGAGAGACC TATATACACA ACACAATTTG CAAGAGATTC 
AAGATGTGGA ATCACAGCAA ATCAGTCATT CACTTTGAAT GGGAACGTGT TATTGACAGT 
CATGTAATTG AAGTGGAGCC CTCATCTGGA GTAATAGAAA TGAATGAGTG TTTTGACATG 
GAGTTGTATC TGACTGGAAG GAGTCCGGGT CATTTTACCT CTACCCTGCA GTGTCACATT 
CTGCACCATC CTAAAACTAT AGGACTTGCC ATTGAGGCCA CATTTAAGGG TCCACATTGC 
TCATTGAATG TACCCAGTCT GGATTTTGGT TTGCTGCAAA TGGGAAATGA GAGAATTTTG 
ACTATTGAAG TCACCAACAA CAGCCTGCTA GATGCCCACT GGAGCCTGGA GGTGCTGTCC 
CAAACCCAGA AGCTCATCGA TGGACAGGTG CATATAAATC CAAATAAAGG TGTTCTGGCT 
GCACTAGAGA GCTGCAGTGT GGATGTGGCC TTCAGAGCCA TGCATTGCCA AGTATTTGAG 
TCAGTTTTAC AACTTTCTGT GCTGAACGGC ACCGGTTGTC ACGTCTCCAT CCATGCCGTT 
GTTCAGTCTC CTCAAGTGTG CTTGCTGAAC TGTGAGTTGC TGTTTGCTGA TCTGTATGTT 
GGGGTTTCAC AGACGGGCAG TGTCACTCTG TTTAACCAAA CACTACTGCC AGCTCACTTC 
ACCTGGGGCA GGCTTCAAGG TTCACAAGCT CATCTCTGCT CCGCAGTCTT CAGTCCCTCT 
GCAGGATCTC TGGGGCCCAA TGCAAAAACT GAAGTCTCTG TGTCATTCAC AGCTCACACT 
GTTGAGGATT TGACTGAAGT GAATGGAGTT TGTGAGGTAA AAGGAATGGA GAATCCACTT 
GTGCTGGGAA TTTTTTCTAA AGCCAAACCT CTCAGAGTTT CCTACTCACT GCTTTGTAAT 
GGTATTAATT CAGGTGATGG CGATCACCAA CCAATCCTGC TGGACTTCAC TGAGCATGAG 
CCAGCCGTCA TTGGGAAATC TGTCAAACGG CAGTTACTGA TAACTAACAA CACTGCTATT 
ACTGCTCCAT TCACTGTCGA CACTGAGTAC TTCACTGGAG ACTGTCCTTC ACAATCAGAA 
GGAAACTCTG AAAGAAGCAG TGCACCTTTC CACTCCATCC AGGCCAAAAA AATACAGCAA 
AAGGCATATG ATGAGTTTGT AAGCCGTCTG CTTTCCCATG GAAGAGGTGC AACATTTTAT 
GTAGAACCAA AAAGTGGGAT GCTTGGCCCA TTCGAGAGTC AAACCATCAA CATCACTACT 
TTCACAAATA TGTGGGGGGA TTATCAGGAC AACCTCATAT GCAAAGTTGG AGATCTAAAT 
CCAACTCACA TTCATATGAA GATGTCTGTG AGGGGCTGTC CAGTTTACTT TCAGATGATT 
GGACCACAGC CTGACAATCA GAATCAGGGT CCAGTTATTA GATTTGGAAG CCATGTTTCT 
GGAGGAGACA CTGTGTCACG CTCTTTACGA CTCATTAACA CCAGTCCATA TGATATCCGT 
ATGGACTGGG TGACCTACAA CCTGGAGGCT GGAGACGGCA AGCTGATAGA TCTGCTGCTT 
GCATGTGGTG AACCTTTCCC TCTGAAGGAT GCAGATGGCA ATGAGGTTGT TGGAGGACTG 
GACTCATCTG TCATGTTCCC GTCCACATGG GATGACAGTC ACACCCCCAG CAGAGAAGGA 
ACAAGCTCAA CTTTTATGAC CCAATCTGAT GATTTTGTAG AGAATGGTGA AGAATGTGAT 
GGAGAAGATG GCAGTGGGGC TTCTGAGTCT CTAGCTCCAG TGAAGAAGCT GTTTTCTGTG 
TTTTTGAAGC CTCATGAAGG GACTCTTTCT GACTACCCAT ACTGCATCAC TCCACAGCAG 
ACTGTAGTTC CAGCAGGGGG CAGTAGCACC ATTCATTTGT CTTTCACTCC CCTGATTCTG 
TCATGTTCAA CCAATCAAAG ATGTTTGGGT TACGCTCTTG GTTTCATGAG CTTAGACTCT 
AAGCTTGCGT TAGTGACTCC AGGCAAGGTG GAGAGAGTCC GGGGCTATGA ACTTCAACCT 
TTGAGGCTGG ACATGCAGGC TAATGTAAAA CCTGCCATTT TGTCAGTGCA GATGGCAGAG 
GATACAGAAG TGCTGGAATT CAGCGCAGCT GCCAGCGACA CACTAGACGG AGCATCACAA 
ACACAGGAAT CCAGGATAAC TCGCACATTC CGGCTAAAGA ACGACACAGA CATGGCCTTG 
AGTTTCAAAC TGAGCACAAA TCCACCATTT TCAGTGCTGC TGCCCCGACA GAGCCTTAAA 
CTGATCAGCA GCTCGCCTGC TTATAGACAC ACACAAGGTC TGTCCGGACC AGGAGATGAG 
ATGAGATTAT TTCTGTTACA GCCCAAAAAC ATCATTCAGG TTAAGGTGGC TTTCCACAAT 
TCCGCTTCAC TCCTGGCCTG TGTAAATGGA GCATGTGAGG AGTCGGACAG TCAGCTCTCT 
GCTTCCCTTC TTTGCAGTGA TGATGGAGGA AAAACTCTAC AGTTTCAGGA GAACTTGACC 
ATTCAGTACA GCAATAACAG CATACAGACA GTCTCTCTTT ACGCTCGTCT GGCATTACCT 
ACACTACACC TGTCCAGTGC CACTGTGGAT TTTGGCACCT GTTATGTTGG ACAAACAACT 
GTCAAAGAGA TTTATCTTCA CAACAGAGGT GGCTCCTGTA GCTACTGGAC CACACTCACA 
GATGGCAATG AAGTGTTCAG GGTAAGTCCG GATTCTGGTT TACTGAAACC TCTTGGAGAT 
CCAGCGTCCT GTAGACAGCT GCTGCAGATC AGCTTCACTC CGAGAGAACA GAAAGAGTTC 
CAGACAATCT TCACAGTGCA GGGGCTTCTT GGAGAGACAC CACTTGTTCT AAAGGTCAGC 
GGAAGAGGAT CATTTGATGA GAGTTTTGTA TCTCCAATAA CTGACAGCTC ACAAAACAAC 
ACTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_683226.4
CDS	197..5305
Translation	MADETEPKALTLKSSEPSINRHTPASKTTQDISHLLASIFNDLYSTEVIGKDTVSNLSKSRRRGNSYHEKYVEELQQVHSEYNRRIQNADMLETHIIQARLQAAAKEEHDRNKILEEVGEAYQQLGLPPVKSAFKWCVDSGLLRSNSLICPLDYTTVHNPVVKSPKARSSPGFAKPTFSYNMHISTELQDDGYTPLPPHAHSAHSLLEYSEETITLSSSPESSSINDTELGLVAKASWMEKTSAKSHAEDRASLKKHKDRHKFLHNPRFLLPNTQHGGKSLIIPGKKKLENVGKSRKNNGRERSLEGHAPIFTANPPVIFFSDYRVGQVYETSVELKNMTASSRHIRMIPPTSPHFSVGLGRFPGEGGIVAPGMSCQYMVRFAPDSLADYEDALVVETQMLYPLIIPIEACRSPPILTLPAVMDCGFCLVGGVKFMEVLCRNEGLSAGTFCVMPKKQWPASSLRSAVKSTFTEQPPFAISPSIFGLHPGQATVIEVVFFPTTPELSTQDFTIVCDNCQVKDITLQGTGQLITVELLSVSGGEDQPEFAELCDITADHYVRFNTTNPHSTLQKTLVVKNNTHLELPYHWQIMKPSLQCLLPGETPDPSSIQHLLDTDSAFSISPVIGILNPAQEHEFLLTYAPKDIKDYHSVCHLVIMDVPSPPKVCDDGTCPDTALHANNVTILEVEVKGSTEPYKILLEPYAVLIPGETYIHNTICKRFKMWNHSKSVIHFEWERVIDSHVIEVEPSSGVIEMNECFDMELYLTGRSPGHFTSTLQCHILHHPKTIGLAIEATFKGPHCSLNVPSLDFGLLQMGNERILTIEVTNNSLLDAHWSLEVLSQTQKLIDGQVHINPNKGVLAALESCSVDVAFRAMHCQVFESVLQLSVLNGTGCHVSIHAVVQSPQVCLLNCELLFADLYVGVSQTGSVTLFNQTLLPAHFTWGRLQGSQAHLCSAVFSPSAGSLGPNAKTEVSVSFTAHTVEDLTEVNGVCEVKGMENPLVLGIFSKAKPLRVSYSLLCNGINSGDGDHQPILLDFTEHEPAVIGKSVKRQLLITNNTAITAPFTVDTEYFTGDCPSQSEGNSERSSAPFHSIQAKKIQQKAYDEFVSRLLSHGRGATFYVEPKSGMLGPFESQTINITTFTNMWGDYQDNLICKVGDLNPTHIHMKMSVRGCPVYFQMIGPQPDNQNQGPVIRFGSHVSGGDTVSRSLRLINTSPYDIRMDWVTYNLEAGDGKLIDLLLACGEPFPLKDADGNEVVGGLDSSVMFPSTWDDSHTPSREGTSSTFMTQSDDFVENGEECDGEDGSGASESLAPVKKLFSVFLKPHEGTLSDYPYCITPQQTVVPAGGSSTIHLSFTPLILSCSTNQRCLGYALGFMSLDSKLALVTPGKVERVRGYELQPLRLDMQANVKPAILSVQMAEDTEVLEFSAAASDTLDGASQTQESRITRTFRLKNDTDMALSFKLSTNPPFSVLLPRQSLKLISSSPAYRHTQGLSGPGDEMRLFLLQPKNIIQVKVAFHNSASLLACVNGACEESDSQLSASLLCSDDGGKTLQFQENLTIQYSNNSIQTVSLYARLALPTLHLSSATVDFGTCYVGQTTVKEIYLHNRGGSCSYWTTLTDGNEVFRVSPDSGLLKPLGDPASCRQLLQISFTPREQKEFQTIFTVQGLLGETPLVLKVSGRGSFDESFVSPITDSSQNNTE

Target ORF information:

RefSeq Version	XM_678134.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio deleted in lung and esophageal cancer 1 (dlec1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_678134.7

ORF Insert Sequence:

ATGGCTGATG AAACTGAACC AAAAGCACTA ACGTTAAAGA GTTCAGAACC GTCAATAAAC 
CGGCACACAC CAGCTTCAAA GACAACTCAG GACATTTCTC ATCTGCTGGC CAGCATATTC 
AATGATCTCT ACAGCACAGA GGTGATTGGG AAGGACACCG TTTCCAATCT CAGTAAATCA 
CGAAGAAGAG GCAACAGCTA CCATGAAAAA TATGTTGAGG AGCTACAACA GGTGCACAGT 
GAATACAACA GACGTATTCA GAATGCTGAT ATGCTAGAGA CACACATCAT ACAGGCCCGT 
CTTCAGGCTG CTGCAAAAGA AGAGCATGAT CGCAACAAAA TCCTGGAAGA AGTCGGTGAG 
GCTTACCAAC AGCTGGGACT TCCCCCAGTA AAATCAGCTT TCAAGTGGTG TGTGGACAGT 
GGACTTTTGA GGAGCAACAG CTTGATTTGC CCACTGGACT ACACAACAGT GCACAACCCA 
GTTGTTAAAA GCCCAAAAGC CAGGTCAAGT CCAGGCTTTG CCAAGCCTAC TTTCTCTTAT 
AATATGCACA TCTCCACTGA ACTGCAGGAT GATGGTTACA CTCCGCTTCC TCCACACGCT 
CATTCAGCAC ACAGCCTGCT TGAATATTCA GAGGAGACAA TCACACTCTC TTCCTCTCCA 
GAGTCCAGCT CCATCAATGA CACAGAGCTA GGTTTGGTGG CGAAGGCTTC TTGGATGGAG 
AAGACCAGTG CTAAGAGTCA TGCAGAAGAC AGGGCTTCCC TGAAAAAGCA CAAAGACCGG 
CATAAGTTTC TGCACAACCC TCGCTTCTTG CTGCCAAACA CTCAGCATGG AGGAAAGTCT 
CTCATCATCC CAGGGAAAAA GAAGCTGGAG AATGTGGGAA AGAGCAGGAA AAACAACGGC 
AGAGAGAGAA GTCTTGAGGG TCATGCGCCT ATTTTCACTG CAAATCCACC AGTGATCTTC 
TTCAGTGACT ACAGAGTAGG ACAAGTCTAT GAGACCTCAG TGGAGCTCAA AAACATGACT 
GCGAGCAGCC GTCACATTCG AATGATTCCT CCAACCTCCC CACACTTCTC TGTGGGTTTG 
GGCCGGTTCC CTGGTGAAGG AGGCATCGTG GCTCCAGGAA TGAGCTGTCA GTATATGGTG 
CGTTTTGCTC CAGATTCTCT AGCAGACTAT GAAGACGCTC TGGTTGTGGA GACGCAGATG 
CTCTATCCTC TCATTATACC AATAGAAGCC TGCAGATCTC CTCCGATACT CACCTTGCCG 
GCTGTCATGG ACTGTGGTTT CTGCCTGGTG GGAGGAGTGA AGTTTATGGA GGTTTTATGT 
CGGAATGAAG GACTGAGTGC TGGAACGTTT TGTGTAATGC CTAAGAAACA GTGGCCTGCT 
TCAAGCCTCA GGTCTGCAGT GAAGTCGACT TTCACAGAAC AGCCTCCCTT TGCTATCAGT 
CCCTCTATTT TCGGCCTTCA CCCTGGACAG GCTACTGTCA TAGAGGTTGT GTTTTTCCCC 
ACAACTCCTG AGCTCTCTAC TCAAGACTTC ACCATTGTCT GTGACAACTG CCAAGTGAAG 
GACATTACGC TCCAAGGTAC TGGTCAACTG ATCACTGTGG AGCTGCTTTC AGTATCAGGT 
GGAGAGGATC AGCCTGAATT TGCAGAATTG TGTGACATCA CAGCTGACCA TTATGTCAGA 
TTTAACACAA CCAATCCACA CTCTACCCTG CAGAAGACAC TGGTTGTCAA AAACAACACA 
CATTTGGAGC TTCCATATCA CTGGCAGATC ATGAAGCCCA GTCTTCAATG CTTGTTGCCC 
GGAGAGACTC CTGACCCTTC CAGCATTCAG CATCTTCTTG ATACAGACAG TGCATTCAGC 
ATCAGTCCTG TTATAGGAAT CCTGAACCCT GCACAGGAAC ACGAGTTCCT GCTCACCTAT 
GCTCCCAAAG ATATAAAAGA TTACCACAGT GTCTGCCACT TGGTGATTAT GGATGTTCCC 
AGTCCACCTA AAGTCTGTGA CGATGGGACA TGTCCTGATA CTGCACTCCA TGCAAACAAT 
GTGACCATTT TAGAGGTTGA AGTGAAAGGT TCTACTGAGC CATACAAGAT CCTTCTTGAG 
CCGTATGCTG TTTTAATACC AGGAGAGACC TATATACACA ACACAATTTG CAAGAGATTC 
AAGATGTGGA ATCACAGCAA ATCAGTCATT CACTTTGAAT GGGAACGTGT TATTGACAGT 
CATGTAATTG AAGTGGAGCC CTCATCTGGA GTAATAGAAA TGAATGAGTG TTTTGACATG 
GAGTTGTATC TGACTGGAAG GAGTCCGGGT CATTTTACCT CTACCCTGCA GTGTCACATT 
CTGCACCATC CTAAAACTAT AGGACTTGCC ATTGAGGCCA CATTTAAGGG TCCACATTGC 
TCATTGAATG TACCCAGTCT GGATTTTGGT TTGCTGCAAA TGGGAAATGA GAGAATTTTG 
ACTATTGAAG TCACCAACAA CAGCCTGCTA GATGCCCACT GGAGCCTGGA GGTGCTGTCC 
CAAACCCAGA AGCTCATCGA TGGACAGGTG CATATAAATC CAAATAAAGG TGTTCTGGCT 
GCACTAGAGA GCTGCAGTGT GGATGTGGCC TTCAGAGCCA TGCATTGCCA AGTATTTGAG 
TCAGTTTTAC AACTTTCTGT GCTGAACGGC ACCGGTTGTC ACGTCTCCAT CCATGCCGTT 
GTTCAGTCTC CTCAAGTGTG CTTGCTGAAC TGTGAGTTGC TGTTTGCTGA TCTGTATGTT 
GGGGTTTCAC AGACGGGCAG TGTCACTCTG TTTAACCAAA CACTACTGCC AGCTCACTTC 
ACCTGGGGCA GGCTTCAAGG TTCACAAGCT CATCTCTGCT CCGCAGTCTT CAGTCCCTCT 
GCAGGATCTC TGGGGCCCAA TGCAAAAACT GAAGTCTCTG TGTCATTCAC AGCTCACACT 
GTTGAGGATT TGACTGAAGT GAATGGAGTT TGTGAGGTAA AAGGAATGGA GAATCCACTT 
GTGCTGGGAA TTTTTTCTAA AGCCAAACCT CTCAGAGTTT CCTACTCACT GCTTTGTAAT 
GGTATTAATT CAGGTGATGG CGATCACCAA CCAATCCTGC TGGACTTCAC TGAGCATGAG 
CCAGCCGTCA TTGGGAAATC TGTCAAACGG CAGTTACTGA TAACTAACAA CACTGCTATT 
ACTGCTCCAT TCACTGTCGA CACTGAGTAC TTCACTGGAG ACTGTCCTTC ACAATCAGAA 
GGAAACTCTG AAAGAAGCAG TGCACCTTTC CACTCCATCC AGGCCAAAAA AATACAGCAA 
AAGGCATATG ATGAGTTTGT AAGCCGTCTG CTTTCCCATG GAAGAGGTGC AACATTTTAT 
GTAGAACCAA AAAGTGGGAT GCTTGGCCCA TTCGAGAGTC AAACCATCAA CATCACTACT 
TTCACAAATA TGTGGGGGGA TTATCAGGAC AACCTCATAT GCAAAGTTGG AGATCTAAAT 
CCAACTCACA TTCATATGAA GATGTCTGTG AGGGGCTGTC CAGTTTACTT TCAGATGATT 
GGACCACAGC CTGACAATCA GAATCAGGGT CCAGTTATTA GATTTGGAAG CCATGTTTCT 
GGAGGAGACA CTGTGTCACG CTCTTTACGA CTCATTAACA CCAGTCCATA TGATATCCGT 
ATGGACTGGG TGACCTACAA CCTGGAGGCT GGAGACGGCA AGCTGATAGA TCTGCTGCTT 
GCATGTGGTG AACCTTTCCC TCTGAAGGAT GCAGATGGCA ATGAGGTTGT TGGAGGACTG 
GACTCATCTG TCATGTTCCC GTCCACATGG GATGACAGTC ACACCCCCAG CAGAGAAGGA 
ACAAGCTCAA CTTTTATGAC CCAATCTGAT GATTTTGTAG AGAATGGTGA AGAATGTGAT 
GGAGAAGATG GCAGTGGGGC TTCTGAGTCT CTAGCTCCAG TGAAGAAGCT GTTTTCTGTG 
TTTTTGAAGC CTCATGAAGG GACTCTTTCT GACTACCCAT ACTGCATCAC TCCACAGCAG 
ACTGTAGTTC CAGCAGGGGG CAGTAGCACC ATTCATTTGT CTTTCACTCC CCTGATTCTG 
TCATGTTCAA CCAATCAAAG ATGTTTGGGT TACGCTCTTG GTTTCATGAG CTTAGACTCT 
AAGCTTGCGT TAGTGACTCC AGGCAAGGTG GAGAGAGTCC GGGGCTATGA ACTTCAACCT 
TTGAGGCTGG ACATGCAGGC TAATGTAAAA CCTGCCATTT TGTCAGTGCA GATGGCAGAG 
GATACAGAAG TGCTGGAATT CAGCGCAGCT GCCAGCGACA CACTAGACGG AGCATCACAA 
ACACAGGAAT CCAGGATAAC TCGCACATTC CGGCTAAAGA ACGACACAGA CATGGCCTTG 
AGTTTCAAAC TGAGCACAAA TCCACCATTT TCAGTGCTGC TGCCCCGACA GAGCCTTAAA 
CTGATCAGCA GCTCGCCTGC TTATAGACAC ACACAAGGTC TGTCCGGACC AGGAGATGAG 
ATGAGATTAT TTCTGTTACA GCCCAAAAAC ATCATTCAGG TTAAGGTGGC TTTCCACAAT 
TCCGCTTCAC TCCTGGCCTG TGTAAATGGA GCATGTGAGG AGTCGGACAG TCAGCTCTCT 
GCTTCCCTTC TTTGCAGTGA TGATGGAGGA AAAACTCTAC AGTTTCAGGA GAACTTGACC 
ATTCAGTACA GCAATAACAG CATACAGACA GTCTCTCTTT ACGCTCGTCT GGCATTACCT 
ACACTACACC TGTCCAGTGC CACTGTGGAT TTTGGCACCT GTTATGTTGG ACAAACAACT 
GTCAAAGAGA TTTATCTTCA CAACAGAGGT GGCTCCTGTA GCTACTGGAC CACACTCACA 
GATGGCAATG AAGTGTTCAG GGTAAGTCCG GATTCTGGTT TACTGAAACC TCTTGGAGAT 
CCAGCGTCCT GTAGACAGCT GCTGCAGATC AGCTTCACTC CGAGAGAACA GAAAGAGTTC 
CAGACAATCT TCACAGTGCA GGGGCTTCTT GGAGAGACAC CACTTGTTCT AAAGGTCAGC 
GGAAGAGGAT CATTTGATGA GAGTTTTGTA TCTCCAATAA CTGACAGCTC ACAAAACAAC 
ACTGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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dlec1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム

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