Dlec1 gene cDNA ORF clone, Mus musculus(house mouse)

mRNA	Protein	Name
XM_006512152.4	XP_006512215.1	deleted in lung and esophageal cancer protein 1 homolog isoform X1
XM_006512154.4	XP_006512217.1	deleted in lung and esophageal cancer protein 1 homolog isoform X3
NM_177117.4	NP_796091.2	deleted in lung and esophageal cancer protein 1 homolog isoform 1
XM_006512153.3	XP_006512216.1	deleted in lung and esophageal cancer protein 1 homolog isoform X2
XM_006512155.3	XP_006512218.1	deleted in lung and esophageal cancer protein 1 homolog isoform X4
XM_006512156.3	XP_006512219.1	deleted in lung and esophageal cancer protein 1 homolog isoform X5
NM_001368821.1	NP_001355750.1	deleted in lung and esophageal cancer protein 1 homolog isoform 3
NM_001368820.1	NP_001355749.1	deleted in lung and esophageal cancer protein 1 homolog isoform 2
XM_006512157.3	XP_006512220.1	deleted in lung and esophageal cancer protein 1 homolog isoform X6
NM_177117.5	NP_796091.2	deleted in lung and esophageal cancer protein 1 homolog isoform 1
XM_006512153.4	XP_006512216.1	deleted in lung and esophageal cancer protein 1 homolog isoform X2
XM_006512152.3	XP_006512215.1	deleted in lung and esophageal cancer protein 1 homolog isoform X1
XM_006512158.3	XP_006512221.1	deleted in lung and esophageal cancer protein 1 homolog isoform X7
XM_006512155.4	XP_006512218.1	deleted in lung and esophageal cancer protein 1 homolog isoform X4
XM_006512156.4	XP_006512219.1	deleted in lung and esophageal cancer protein 1 homolog isoform X5
XM_006512158.4	XP_006512221.1	deleted in lung and esophageal cancer protein 1 homolog isoform X6
XM_006512154.3	XP_006512217.1	deleted in lung and esophageal cancer protein 1 homolog isoform X3
NM_177117.3	NP_796091.2	deleted in lung and esophageal cancer protein 1 homolog
XM_006512159.1	XP_006512222.1	deleted in lung and esophageal cancer protein 1 homolog isoform X7

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu43446	XM_006512158.4 Latest version!	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OMu43447	XM_006512159.1 Latest version!	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu84577	NM_001368820.1 Latest version!	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OMu43440	XM_006512152.4 Latest version!	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu43441	XM_006512153.4 Latest version!	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu43442	XM_006512154.4 Latest version!	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu43443	XM_006512155.4 Latest version!	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu43444	XM_006512156.4 Latest version!	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OMu05953	NM_177117.5 Latest version!	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1280.30 $1829.00
OMu43445	NM_001368821.1 Latest version!	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OMu43445	XM_006512157.3 Latest version!	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
View Old Accession Versions >>
OMu05953	NM_177117.4	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1280.30 $1829.00
OMu43441	XM_006512153.3	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu43440	XM_006512152.3	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu43443	XM_006512155.3	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu43444	XM_006512156.3	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu43446	XM_006512158.3	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OMu43442	XM_006512154.3	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OMu05953	NM_177117.3	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1280.30 $1829.00
Hide Old Accession Versions >>

Dlec1 ( XM_006512158.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512221.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OMu43446
Clone ID Related Accession (Same CDS sequence)	XM_006512158.3 , XM_006512158.4
Accession Version	XM_006512158.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512158.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGCAACC 
CTCTGTGACC TCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512221.1
CDS	72..3209
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEATLCDLR

Target ORF information:

RefSeq Version	XM_006512158.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512158.4

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGCAACC 
CTCTGTGACC TCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( XM_006512158.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512221.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OMu43446
Clone ID Related Accession (Same CDS sequence)	XM_006512158.3 , XM_006512158.4
Accession Version	XM_006512158.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3138bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512158.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGCAACC 
CTCTGTGACC TCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512221.1
CDS	983..4120
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEATLCDLR

Target ORF information:

RefSeq Version	XM_006512158.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512158.3

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGCAACC 
CTCTGTGACC TCAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( XM_006512159.1 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512222.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X7, mRNA.

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CloneID	OMu43447
Clone ID Related Accession (Same CDS sequence)	XM_006512159.1
Accession Version	XM_006512159.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTCTAG AAGCTGTGCC CGAGCCCTTG AGCTCAGGGT TGGAGAACCT GGGGGACTAC 
TCCTACTCAG TGGATGACAT GATCGTCCTG GAAATCGAGG TGAAAGGCTT GGCGGAACCC 
TTCCAGGTCC TTTTAGAGCC GTACGCCCTC ATCATCCCTG GGGAGAACTA CATTGGCATC 
ACCGTGAAGA AAGATTTTAA GATGTGGAAC AACAGCAAGT CACCCATCAG ATACATGTGG 
GGGAAGATCA GCCACTGCCA TATCATCGAA GTGGAGCCCT GCACTGGGGT GATAGAGCCC 
AATGAGGTCG GAGATTTTGA GTTAAACCTA ACGGGTGGGG TGCCTGGCCC AACCAGCCAG 
GACCTGGAGT GTGAAATCAA AGACTCACCA TGCCCGGTGG TACTCCACAT TGAGGCCTCA 
TTTAAGGGAC CTGCCTTGAC CATTGATGTC CCAGCCCTTC AGTTTGGTCT GCTTCGCCTG 
GGAAAGAAGG CTTCCATCTC CATCCAGATC CGGAATGTCA GCCAGCTGTC CGCCGTGTGG 
CATTTGAAGG AGAGCCCAGT CTGCCTGGCA GAGAGAGATG AGGATGTGTC CCCCTTCGAC 
ATCGAGCCTT TCTGCGGCCA GCTTCTCCCT CTGGGGGAGT GCAGAGTGAC CATCACCCTT 
GAGGCCTCCC AGTGTCAGCG GCTGCAGACA GTCCTGGAGC TGGAAGTGGA GAACGGGTCC 
GGGAGCTACC TTCCCGTGTA CGCTGAGGTG CAGCAGCCTC ACGTGTACCT GAAGAACAGC 
CACTTGGAGT TCAGCAACCT CTACCTGGGC GTGTCCACAA AGTCAAGGAC CACGCTGGTC 
AATGGCACAC TCCTGCCCAC CCGGTTCCAC TGGGGAAAGC TTCTGGGACC TCAAGCAGAT 
TTGTGCACAA TGACAGTCTC TCCCACACAG GGCCTGCTGG GCCCCAGTGA AGAGCTCCAA 
CTCACCTTGG AGTTGACGAC CCACACAGAG GAAAAGCTGA CCAATTTGGT TCTCCTTTGT 
CACGTGTCTG GCATGGAGAA GCCGCTGGTC CTGGGCATCT CTGGGAAGCC CCTGGGACTT 
CAGGTGACCA TCTCCATAGT GGAATCTGAC GGAAGCAGCA CCACCAGCAC TACACAGGAG 
GAGCTGCGCT TGGACTTCGG CTCGAAGGTG CCACTGAGGA CGCCCGTGAT TCGCCATATC 
ATCCTGACAA ACTGCTCCCC GATACAGACC CCATTCACCC TCACACTGGA GTACTTCGGG 
AGCTCCCAGG ACAGCCTGTA CAAGAAGACC AGCGTCCCTG ACGTGCCTCC TGCCCTGCTG 
AAGACAGCAC GCATCCAGCA GCACGTGGCC AAGAAGGAAA GGCTGGACTT TGTGGAGAGC 
ATGTTATCTC ACAGGAAAGG AGCTGCCTTC CTTCCCCACA TCTCCCAGGG CATGCTGGGA 
GCCTACCAGG AGCTATGTAT TGACATCACA GGCTGTGCCA ACATGTGGGG CGAGTACTGG 
GATGAGCTCC TCTGCGTGGT GGGAGACCTG CCGGTCACTG TCATCCCGGT ATACATGGCC 
GTGGTGGGCT GCCCTATCAG CTCCCTGAGG AATACGTGCT ACAGTGTGGC CCCATTCCAG 
AAGGAGCCTA TCACCAGGTT TGGGACCCAG ATCTCTGGAG GAGATACAGT CACCAGATCT 
CTCCGTCTGT ATAACTCCAG TCACTGTGAC ATCCGCCTAG ACTGGGAGAC CTACATCCCA 
GAAGAAAAGG CGGACCGGCT GCTGGAGCTG CTTGTGTTTT ATGGGCCACC CTTCCCACTG 
CGAGATCAAG ACGGCAGTGA GATTGTGTGC CCTGAGACCT CGGAGGCCAG CTTACCAGGG 
TCTCCATGCC CCTCCAATGT CTCAGTGTCT AGCCACACTG TCCCCTCAAC GGGCAGCTGC 
AGTGGCGGCG GCGGCGGCAC CAGGGCCGAA GAGCAGATCA TGTCTGTCAT CCTGCAGAGG 
CACGAGGCAG TGCCCGCCGG CCACATATAC CGTATCAGCC CCAAGCAGTT GGTCATCCCA 
GCTGGAGACC ACAGGACCAT CTACATCTCC TTCACGCCCA CGGTGCTCGA CCCAGGGGTC 
ATGCACAAGG TGGGGTATAC TGGCTATGCC CTGGGCTTCA TGAGCTTGGA TAAGGAGACA 
GACAGACAGA TTCCAGGCCG AATGCGACGC CTTCAGGACT TTGCCGTGAG CCCCCTGAGA 
CTGGATCTGA ATGGCCACGT GAGACACGCG CAGCTGCACG TCGAGCTGGA CAGCAGTGGC 
TACTTGGAAT TCTGGTGCCA GGCCAGCGAC CTTATCCCCC AAAATCCCTG CAGTGGGGTG 
CTAAGTGACC GGATTACCAC CCGCCATCTG AAACTGACCA ACACCACTGA AATCCTGTAT 
GACTTCCAGG CCCGGGTCTC CAGGCCCTTC TTCATTTCTC AAGGTGGGGC CAGCTGGGAC 
AGCAGAGCCC CTCATCAGTG TGAAGAAGGG ACGGCCTCCA CAGGCCAGTG GCTGATGATC 
CGTCCTCAGG ACAACATGCT GGTGGATGTG TCCTTCTCAC TGTCCCTGGA ACTGCTGTCC 
TATCAGAAAC TCTCGGCCGA CCAGATGTTG CCCGGAGTGG ATATTCAGGA GAGCGAGAAC 
GGAGAGAGAA AGATGGTGTT CACACAGAAC CTACTCCTGG GCTTCACCAA CCAGACTGTT 
CAGGAGGTGC CCCTGCGGGC CTTTGTGACC CTGCCAGCGC CACAGCTCTC CACCAGCTGG 
GTGGACTTCG GGACCTGTCT CGTGAACCAG CAGCACACCC GGCAGATCTA CCTGATGAAC 
CTCAGCTGCT GCCTGAGCTA CTGGACGGTG CTGTTGGGAC AAGAGGAACC AGCCAGGGAG 
GATCATGCGT TTGGGGTCTC CCCAGGCAGT GGACTGCTGG AGGCCCGAGC CACCAATGCA 
CCCCCTACCT CCAGCCCACT TCAGGTGTTC TTCAACCCTA GGAGCAGCAT CCTCTATGAA 
TCTACACTGG TGGTGGAAGG CGTGCTGAGC GAGAAGCCCT GTGTCCTGAG GCTTCGGGGC 
CTAGGCTCTT ACGATGAAAG ATACGTGAAA TTCCATGAGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512222.1
CDS	118..3222
Translation	MVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTGSCSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVDVSFSLSLELLSYQKLSADQMLPGVDIQESENGERKMVFTQNLLLGFTNQTVQEVPLRAFVTLPAPQLSTSWVDFGTCLVNQQHTRQIYLMNLSCCLSYWTVLLGQEEPAREDHAFGVSPGSGLLEARATNAPPTSSPLQVFFNPRSSILYESTLVVEGVLSEKPCVLRLRGLGSYDERYVKFHEF

Target ORF information:

RefSeq Version	XM_006512159.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512159.1

ORF Insert Sequence:

ATGGTTCTAG AAGCTGTGCC CGAGCCCTTG AGCTCAGGGT TGGAGAACCT GGGGGACTAC 
TCCTACTCAG TGGATGACAT GATCGTCCTG GAAATCGAGG TGAAAGGCTT GGCGGAACCC 
TTCCAGGTCC TTTTAGAGCC GTACGCCCTC ATCATCCCTG GGGAGAACTA CATTGGCATC 
ACCGTGAAGA AAGATTTTAA GATGTGGAAC AACAGCAAGT CACCCATCAG ATACATGTGG 
GGGAAGATCA GCCACTGCCA TATCATCGAA GTGGAGCCCT GCACTGGGGT GATAGAGCCC 
AATGAGGTCG GAGATTTTGA GTTAAACCTA ACGGGTGGGG TGCCTGGCCC AACCAGCCAG 
GACCTGGAGT GTGAAATCAA AGACTCACCA TGCCCGGTGG TACTCCACAT TGAGGCCTCA 
TTTAAGGGAC CTGCCTTGAC CATTGATGTC CCAGCCCTTC AGTTTGGTCT GCTTCGCCTG 
GGAAAGAAGG CTTCCATCTC CATCCAGATC CGGAATGTCA GCCAGCTGTC CGCCGTGTGG 
CATTTGAAGG AGAGCCCAGT CTGCCTGGCA GAGAGAGATG AGGATGTGTC CCCCTTCGAC 
ATCGAGCCTT TCTGCGGCCA GCTTCTCCCT CTGGGGGAGT GCAGAGTGAC CATCACCCTT 
GAGGCCTCCC AGTGTCAGCG GCTGCAGACA GTCCTGGAGC TGGAAGTGGA GAACGGGTCC 
GGGAGCTACC TTCCCGTGTA CGCTGAGGTG CAGCAGCCTC ACGTGTACCT GAAGAACAGC 
CACTTGGAGT TCAGCAACCT CTACCTGGGC GTGTCCACAA AGTCAAGGAC CACGCTGGTC 
AATGGCACAC TCCTGCCCAC CCGGTTCCAC TGGGGAAAGC TTCTGGGACC TCAAGCAGAT 
TTGTGCACAA TGACAGTCTC TCCCACACAG GGCCTGCTGG GCCCCAGTGA AGAGCTCCAA 
CTCACCTTGG AGTTGACGAC CCACACAGAG GAAAAGCTGA CCAATTTGGT TCTCCTTTGT 
CACGTGTCTG GCATGGAGAA GCCGCTGGTC CTGGGCATCT CTGGGAAGCC CCTGGGACTT 
CAGGTGACCA TCTCCATAGT GGAATCTGAC GGAAGCAGCA CCACCAGCAC TACACAGGAG 
GAGCTGCGCT TGGACTTCGG CTCGAAGGTG CCACTGAGGA CGCCCGTGAT TCGCCATATC 
ATCCTGACAA ACTGCTCCCC GATACAGACC CCATTCACCC TCACACTGGA GTACTTCGGG 
AGCTCCCAGG ACAGCCTGTA CAAGAAGACC AGCGTCCCTG ACGTGCCTCC TGCCCTGCTG 
AAGACAGCAC GCATCCAGCA GCACGTGGCC AAGAAGGAAA GGCTGGACTT TGTGGAGAGC 
ATGTTATCTC ACAGGAAAGG AGCTGCCTTC CTTCCCCACA TCTCCCAGGG CATGCTGGGA 
GCCTACCAGG AGCTATGTAT TGACATCACA GGCTGTGCCA ACATGTGGGG CGAGTACTGG 
GATGAGCTCC TCTGCGTGGT GGGAGACCTG CCGGTCACTG TCATCCCGGT ATACATGGCC 
GTGGTGGGCT GCCCTATCAG CTCCCTGAGG AATACGTGCT ACAGTGTGGC CCCATTCCAG 
AAGGAGCCTA TCACCAGGTT TGGGACCCAG ATCTCTGGAG GAGATACAGT CACCAGATCT 
CTCCGTCTGT ATAACTCCAG TCACTGTGAC ATCCGCCTAG ACTGGGAGAC CTACATCCCA 
GAAGAAAAGG CGGACCGGCT GCTGGAGCTG CTTGTGTTTT ATGGGCCACC CTTCCCACTG 
CGAGATCAAG ACGGCAGTGA GATTGTGTGC CCTGAGACCT CGGAGGCCAG CTTACCAGGG 
TCTCCATGCC CCTCCAATGT CTCAGTGTCT AGCCACACTG TCCCCTCAAC GGGCAGCTGC 
AGTGGCGGCG GCGGCGGCAC CAGGGCCGAA GAGCAGATCA TGTCTGTCAT CCTGCAGAGG 
CACGAGGCAG TGCCCGCCGG CCACATATAC CGTATCAGCC CCAAGCAGTT GGTCATCCCA 
GCTGGAGACC ACAGGACCAT CTACATCTCC TTCACGCCCA CGGTGCTCGA CCCAGGGGTC 
ATGCACAAGG TGGGGTATAC TGGCTATGCC CTGGGCTTCA TGAGCTTGGA TAAGGAGACA 
GACAGACAGA TTCCAGGCCG AATGCGACGC CTTCAGGACT TTGCCGTGAG CCCCCTGAGA 
CTGGATCTGA ATGGCCACGT GAGACACGCG CAGCTGCACG TCGAGCTGGA CAGCAGTGGC 
TACTTGGAAT TCTGGTGCCA GGCCAGCGAC CTTATCCCCC AAAATCCCTG CAGTGGGGTG 
CTAAGTGACC GGATTACCAC CCGCCATCTG AAACTGACCA ACACCACTGA AATCCTGTAT 
GACTTCCAGG CCCGGGTCTC CAGGCCCTTC TTCATTTCTC AAGGTGGGGC CAGCTGGGAC 
AGCAGAGCCC CTCATCAGTG TGAAGAAGGG ACGGCCTCCA CAGGCCAGTG GCTGATGATC 
CGTCCTCAGG ACAACATGCT GGTGGATGTG TCCTTCTCAC TGTCCCTGGA ACTGCTGTCC 
TATCAGAAAC TCTCGGCCGA CCAGATGTTG CCCGGAGTGG ATATTCAGGA GAGCGAGAAC 
GGAGAGAGAA AGATGGTGTT CACACAGAAC CTACTCCTGG GCTTCACCAA CCAGACTGTT 
CAGGAGGTGC CCCTGCGGGC CTTTGTGACC CTGCCAGCGC CACAGCTCTC CACCAGCTGG 
GTGGACTTCG GGACCTGTCT CGTGAACCAG CAGCACACCC GGCAGATCTA CCTGATGAAC 
CTCAGCTGCT GCCTGAGCTA CTGGACGGTG CTGTTGGGAC AAGAGGAACC AGCCAGGGAG 
GATCATGCGT TTGGGGTCTC CCCAGGCAGT GGACTGCTGG AGGCCCGAGC CACCAATGCA 
CCCCCTACCT CCAGCCCACT TCAGGTGTTC TTCAACCCTA GGAGCAGCAT CCTCTATGAA 
TCTACACTGG TGGTGGAAGG CGTGCTGAGC GAGAAGCCCT GTGTCCTGAG GCTTCGGGGC 
CTAGGCTCTT ACGATGAAAG ATACGTGAAA TTCCATGAGT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( NM_001368820.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001355749.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant 2, mRNA.

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CloneID	OMu84577
Clone ID Related Accession (Same CDS sequence)	NM_001368820.1
Accession Version	NM_001368820.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4758bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC055818.9. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC145278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GACAAGCAGG CCAAAGAATA AGAAGTGGAT GGAACACCTG 
CAGGTGCCGC AGAGAGCCCT GGACAGGCGG CTGCTCGCCA GCATGGAGAA CAGGAATCAC 
TTCCTGAAAA ACCCTCGGTT TTTCCCGCCT AACACACCAC ATGGAGGCAA GTCTCTCATC 
GTCCCTTTGA GCAAGCCAGA GAGAAGAGCA TCCCTGAATG CAGAGCCTGA GTGGAGCTGT 
ACAGATGCCC CCGTGTTCCT GGCGAAGCCA TCAGTTGGAT TTTTCACAGA TTACGAAATT 
GGACCCGTTT ACGAGATGGT GATCGCTCTA CAGAACACCA CCTCCACCAG CCGATTCTTG 
CGTGTCCTCC CACCGTCCTC TCCGTACTTC GCTCTGGGAC TTGGGATGTT CCCAGGAAAA 
GGCGGAATAG TGGCTCCCGG GATGACCTGC CAGTACATCG TCCAGTTCAT CCCGGACTGT 
CTTGGCGATT TTGATGATTT CATTTTGGTG GAGACACAGT CTCCCCACAC GCTGGTAATC 
CCCCTGCAGG CCCGGAGACC GCCGCCAGTG CTGACCTTGT CACCAGTGTT GGACTGCGGC 
CACTGCCTCA TTGGAGGGGT GAAGATAACC AAATTCATCT GCAAAAATGT GGGCTTCAGC 
GTCGGCAAAT TCTGTATCAT GCCCCAAAAG AGCTGGCCGC CACCGAGTTT CCGGGCTGTT 
GCAACTACTG GCTTTGTAGA GCAGCCTCCT TTTGGAGTAA TGCCTTCCGT GTTTGAGCTG 
GCCCCAGGGT ACGCCATACT GTTGGAGGTC TTGTTCCTCC CTACTGGCCT AGGACAGGCA 
GAAGAGACCT TCATCATCGT GTGTGACAAC TGTCAGATAA AGGAGGTGGT CGTCGTAGGG 
ACAGGGCAGC TGGTGGCTTT GGATCTGATT CATATTTCTG GTGGAAAAAA TGAGCCAGAC 
CCAGGAGAGC TGAAAGATTT AACAGCCCAG TACTTTATCC GGTTTGAGCC TGAAAACGTC 
CAGTCCACCG CAAAGAAGCA GCTCATCATC AGAAATGCTA CACACGTGGA GCTGGCCTTC 
CACTGGCAGA TCATGAAACC CAACCTGCAG CCTCTGATGC CTGGAGAGAC ACACAGCTCA 
GACAGCATCA AATGCCACCC TGACAGGGAG ACAGCCTTCT CCATCATCCC CGAGAAGGGC 
ATCCTCGAGT CCCACTCGGA CCAAGAGTTC ATCCTGAGTT TTTCACCTTA CAAGCTCAAG 
TGTTTCCACA GTGTACTGCA AATGGTTCTA GAAGCTGTGC CCGAGCCCTT GAGCTCAGGG 
TTGGAGAACC TGGGGGACTA CTCCTACTCA GTGGATGACA TGATCGTCCT GGAAATCGAG 
GTGAAAGGCT TGGCGGAACC CTTCCAGGTC CTTTTAGAGC CGTACGCCCT CATCATCCCT 
GGGGAGAACT ACATTGGCAT CACCGTGAAG AAAGATTTTA AGATGTGGAA CAACAGCAAG 
TCACCCATCA GATACATGTG GGGGAAGATC AGCCACTGCC ATATCATCGA AGTGGAGCCC 
TGCACTGGGG TGATAGAGCC CAATGAGGTC GGAGATTTTG AGTTAAACCT AACGGGTGGG 
GTGCCTGGCC CAACCAGCCA GGACCTGGAG TGTGAAATCA AAGACTCACC ATGCCCGGTG 
GTACTCCACA TTGAGGCCTC ATTTAAGGGA CCTGCCTTGA CCATTGATGT CCCAGCCCTT 
CAGTTTGGTC TGCTTCGCCT GGGAAAGAAG GCTTCCATCT CCATCCAGAT CCGGAATGTC 
AGCCAGCTGT CCGCCGTGTG GCATTTGAAG GAGAGCCCAG TCTGCCTGGC AGAGAGAGAT 
GAGGATGTGT CCCCCTTCGA CATCGAGCCT TTCTGCGGCC AGCTTCTCCC TCTGGGGGAG 
TGCAGAGTGA CCATCACCCT TGAGGCCTCC CAGTGTCAGC GGCTGCAGAC AGTCCTGGAG 
CTGGAAGTGG AGAACGGGTC CGGGAGCTAC CTTCCCGTGT ACGCTGAGGT GCAGCAGCCT 
CACGTGTACC TGAAGAACAG CCACTTGGAG TTCAGCAACC TCTACCTGGG CGTGTCCACA 
AAGTCAAGGA CCACGCTGGT CAATGGCACA CTCCTGCCCA CCCGGTTCCA CTGGGGAAAG 
CTTCTGGGAC CTCAAGCAGA TTTGTGCACA ATGACAGTCT CTCCCACACA GGGCCTGCTG 
GGCCCCAGTG AAGAGCTCCA ACTCACCTTG GAGTTGACGA CCCACACAGA GGAAAAGCTG 
ACCAATTTGG TTCTCCTTTG TCACGTGTCT GGCATGGAGA AGCCGCTGGT CCTGGGCATC 
TCTGGGAAGC CCCTGGGACT TCAGGTGACC ATCTCCATAG TGGAATCTGA CGGAAGCAGC 
ACCACCAGCA CTACACAGGA GGAGCTGCGC TTGGACTTCG GCTCGAAGGT GCCACTGAGG 
ACGCCCGTGA TTCGCCATAT CATCCTGACA AACTGCTCCC CGATACAGAC CCCATTCACC 
CTCACACTGG AGTACTTCGG GAGCTCCCAG GACAGCCTGT ACAAGAAGAC CAGCGTCCCT 
GACGTGCCTC CTGCCCTGCT GAAGACAGCA CGCATCCAGC AGCACGTGGC CAAGAAGGAA 
AGGCTGGACT TTGTGGAGAG CATGTTATCT CACAGGAAAG GAGCTGCCTT CCTTCCCCAC 
ATCTCCCAGG GCATGCTGGG AGCCTACCAG GAGCTATGTA TTGACATCAC AGGCTGTGCC 
AACATGTGGG GCGAGTACTG GGATGAGCTC CTCTGCGTGG TGGGAGACCT GCCGGTCACT 
GTCATCCCGG TATACATGGC CGTGGTGGGC TGCCCTATCA GCTCCCTGAG GAATACGTGC 
TACAGTGTGG CCCCATTCCA GAAGGAGCCT ATCACCAGGT TTGGGACCCA GATCTCTGGA 
GGAGATACAG TCACCAGATC TCTCCGTCTG TATAACTCCA GTCACTGTGA CATCCGCCTA 
GACTGGGAGA CCTACATCCC AGAAGAAAAG GCGGACCGGC TGCTGGAGCT GCTTGTGTTT 
TATGGGCCAC CCTTCCCACT GCGAGATCAA GACGGCAGTG AGATTGTGTG CCCTGAGACC 
TCGGAGGCCA GCTTACCAGG GTCTCCATGC CCCTCCAATG TCTCAGTGTC TAGCCACACT 
GTCCCCTCAA CGGGCAGCTG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTCAGTGC AGGGGTGGAG 
CGTCTCCCCC ACCACCCCCG CCTCTATAGG CAACACACAG GCACATCCTG CCCACATCTG 
GGAACGAGGT ACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355749.1
CDS	7..4764
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTGSCSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVSAGVERLPHHPRLYRQHTGTSCPHLGTRYK

Target ORF information:

RefSeq Version	NM_001368820.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368820.1

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GACAAGCAGG CCAAAGAATA AGAAGTGGAT GGAACACCTG 
CAGGTGCCGC AGAGAGCCCT GGACAGGCGG CTGCTCGCCA GCATGGAGAA CAGGAATCAC 
TTCCTGAAAA ACCCTCGGTT TTTCCCGCCT AACACACCAC ATGGAGGCAA GTCTCTCATC 
GTCCCTTTGA GCAAGCCAGA GAGAAGAGCA TCCCTGAATG CAGAGCCTGA GTGGAGCTGT 
ACAGATGCCC CCGTGTTCCT GGCGAAGCCA TCAGTTGGAT TTTTCACAGA TTACGAAATT 
GGACCCGTTT ACGAGATGGT GATCGCTCTA CAGAACACCA CCTCCACCAG CCGATTCTTG 
CGTGTCCTCC CACCGTCCTC TCCGTACTTC GCTCTGGGAC TTGGGATGTT CCCAGGAAAA 
GGCGGAATAG TGGCTCCCGG GATGACCTGC CAGTACATCG TCCAGTTCAT CCCGGACTGT 
CTTGGCGATT TTGATGATTT CATTTTGGTG GAGACACAGT CTCCCCACAC GCTGGTAATC 
CCCCTGCAGG CCCGGAGACC GCCGCCAGTG CTGACCTTGT CACCAGTGTT GGACTGCGGC 
CACTGCCTCA TTGGAGGGGT GAAGATAACC AAATTCATCT GCAAAAATGT GGGCTTCAGC 
GTCGGCAAAT TCTGTATCAT GCCCCAAAAG AGCTGGCCGC CACCGAGTTT CCGGGCTGTT 
GCAACTACTG GCTTTGTAGA GCAGCCTCCT TTTGGAGTAA TGCCTTCCGT GTTTGAGCTG 
GCCCCAGGGT ACGCCATACT GTTGGAGGTC TTGTTCCTCC CTACTGGCCT AGGACAGGCA 
GAAGAGACCT TCATCATCGT GTGTGACAAC TGTCAGATAA AGGAGGTGGT CGTCGTAGGG 
ACAGGGCAGC TGGTGGCTTT GGATCTGATT CATATTTCTG GTGGAAAAAA TGAGCCAGAC 
CCAGGAGAGC TGAAAGATTT AACAGCCCAG TACTTTATCC GGTTTGAGCC TGAAAACGTC 
CAGTCCACCG CAAAGAAGCA GCTCATCATC AGAAATGCTA CACACGTGGA GCTGGCCTTC 
CACTGGCAGA TCATGAAACC CAACCTGCAG CCTCTGATGC CTGGAGAGAC ACACAGCTCA 
GACAGCATCA AATGCCACCC TGACAGGGAG ACAGCCTTCT CCATCATCCC CGAGAAGGGC 
ATCCTCGAGT CCCACTCGGA CCAAGAGTTC ATCCTGAGTT TTTCACCTTA CAAGCTCAAG 
TGTTTCCACA GTGTACTGCA AATGGTTCTA GAAGCTGTGC CCGAGCCCTT GAGCTCAGGG 
TTGGAGAACC TGGGGGACTA CTCCTACTCA GTGGATGACA TGATCGTCCT GGAAATCGAG 
GTGAAAGGCT TGGCGGAACC CTTCCAGGTC CTTTTAGAGC CGTACGCCCT CATCATCCCT 
GGGGAGAACT ACATTGGCAT CACCGTGAAG AAAGATTTTA AGATGTGGAA CAACAGCAAG 
TCACCCATCA GATACATGTG GGGGAAGATC AGCCACTGCC ATATCATCGA AGTGGAGCCC 
TGCACTGGGG TGATAGAGCC CAATGAGGTC GGAGATTTTG AGTTAAACCT AACGGGTGGG 
GTGCCTGGCC CAACCAGCCA GGACCTGGAG TGTGAAATCA AAGACTCACC ATGCCCGGTG 
GTACTCCACA TTGAGGCCTC ATTTAAGGGA CCTGCCTTGA CCATTGATGT CCCAGCCCTT 
CAGTTTGGTC TGCTTCGCCT GGGAAAGAAG GCTTCCATCT CCATCCAGAT CCGGAATGTC 
AGCCAGCTGT CCGCCGTGTG GCATTTGAAG GAGAGCCCAG TCTGCCTGGC AGAGAGAGAT 
GAGGATGTGT CCCCCTTCGA CATCGAGCCT TTCTGCGGCC AGCTTCTCCC TCTGGGGGAG 
TGCAGAGTGA CCATCACCCT TGAGGCCTCC CAGTGTCAGC GGCTGCAGAC AGTCCTGGAG 
CTGGAAGTGG AGAACGGGTC CGGGAGCTAC CTTCCCGTGT ACGCTGAGGT GCAGCAGCCT 
CACGTGTACC TGAAGAACAG CCACTTGGAG TTCAGCAACC TCTACCTGGG CGTGTCCACA 
AAGTCAAGGA CCACGCTGGT CAATGGCACA CTCCTGCCCA CCCGGTTCCA CTGGGGAAAG 
CTTCTGGGAC CTCAAGCAGA TTTGTGCACA ATGACAGTCT CTCCCACACA GGGCCTGCTG 
GGCCCCAGTG AAGAGCTCCA ACTCACCTTG GAGTTGACGA CCCACACAGA GGAAAAGCTG 
ACCAATTTGG TTCTCCTTTG TCACGTGTCT GGCATGGAGA AGCCGCTGGT CCTGGGCATC 
TCTGGGAAGC CCCTGGGACT TCAGGTGACC ATCTCCATAG TGGAATCTGA CGGAAGCAGC 
ACCACCAGCA CTACACAGGA GGAGCTGCGC TTGGACTTCG GCTCGAAGGT GCCACTGAGG 
ACGCCCGTGA TTCGCCATAT CATCCTGACA AACTGCTCCC CGATACAGAC CCCATTCACC 
CTCACACTGG AGTACTTCGG GAGCTCCCAG GACAGCCTGT ACAAGAAGAC CAGCGTCCCT 
GACGTGCCTC CTGCCCTGCT GAAGACAGCA CGCATCCAGC AGCACGTGGC CAAGAAGGAA 
AGGCTGGACT TTGTGGAGAG CATGTTATCT CACAGGAAAG GAGCTGCCTT CCTTCCCCAC 
ATCTCCCAGG GCATGCTGGG AGCCTACCAG GAGCTATGTA TTGACATCAC AGGCTGTGCC 
AACATGTGGG GCGAGTACTG GGATGAGCTC CTCTGCGTGG TGGGAGACCT GCCGGTCACT 
GTCATCCCGG TATACATGGC CGTGGTGGGC TGCCCTATCA GCTCCCTGAG GAATACGTGC 
TACAGTGTGG CCCCATTCCA GAAGGAGCCT ATCACCAGGT TTGGGACCCA GATCTCTGGA 
GGAGATACAG TCACCAGATC TCTCCGTCTG TATAACTCCA GTCACTGTGA CATCCGCCTA 
GACTGGGAGA CCTACATCCC AGAAGAAAAG GCGGACCGGC TGCTGGAGCT GCTTGTGTTT 
TATGGGCCAC CCTTCCCACT GCGAGATCAA GACGGCAGTG AGATTGTGTG CCCTGAGACC 
TCGGAGGCCA GCTTACCAGG GTCTCCATGC CCCTCCAATG TCTCAGTGTC TAGCCACACT 
GTCCCCTCAA CGGGCAGCTG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTCAGTGC AGGGGTGGAG 
CGTCTCCCCC ACCACCCCCG CCTCTATAGG CAACACACAG GCACATCCTG CCCACATCTG 
GGAACGAGGT ACAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( XM_006512152.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512215.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X1, mRNA.

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CloneID	OMu43440
Clone ID Related Accession (Same CDS sequence)	XM_006512152.4 , XM_006512152.3
Accession Version	XM_006512152.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512152.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGGCAG CTGCAGTGGC GGCGGCGGCG GCACCAGGGC CGAAGAGCAG 
ATCATGTCTG TCATCCTGCA GAGGCACGAG GCAGTGCCCG CCGGCCACAT ATACCGTATC 
AGCCCCAAGC AGTTGGTCAT CCCAGCTGGA GACCACAGGA CCATCTACAT CTCCTTCACG 
CCCACGGTGC TCGACCCAGG GGTCATGCAC AAGGTGGGGT ATACTGGCTA TGCCCTGGGC 
TTCATGAGCT TGGATAAGGA GACAGACAGA CAGATTCCAG GCCGAATGCG ACGCCTTCAG 
GACTTTGCCG TGAGCCCCCT GAGACTGGAT CTGAATGGCC ACGTGAGACA CGCGCAGCTG 
CACGTCGAGC TGGACAGCAG TGGCTACTTG GAATTCTGGT GCCAGGCCAG CGACCTTATC 
CCCCAAAATC CCTGCAGTGG GGTGCTAAGT GACCGGATTA CCACCCGCCA TCTGAAACTG 
ACCAACACCA CTGAAATCCT GTATGACTTC CAGGCCCGGG TCTCCAGGCC CTTCTTCATT 
TCTCAAGGTG GGGCCAGCTG GGACAGCAGA GCCCCTCATC AGTGTGAAGA AGGGACGGCC 
TCCACAGGCC AGTGGCTGAT GATCCGTCCT CAGGACAACA TGCTGGTGGA TGTGTCCTTC 
TCACTGTCCC TGGAACTGCT GTCCTATCAG AAACTCTCGG CCGACCAGAT GTTGCCCGGA 
GTGGATATTC AGGAGAGCGA GAACGGAGAG AGAAAGATGG TGTTCACACA GAACCTACTC 
CTGGGCTTCA CCAACCAGAC TGTTCAGGAG GTGCCCCTGC GGGCCTTTGT GACCCTGCCA 
GCGCCACAGC TCTCCACCAG CTGGGTGGAC TTCGGGACCT GTCTCGTGAA CCAGCAGCAC 
ACCCGGCAGA TCTACCTGAT GAACCTCAGC TGCTGCCTGA GCTACTGGAC GGTGCTGTTG 
GGACAAGAGG AACCAGCCAG GGAGGATCAT GCGTTTGGGG TCTCCCCAGG CAGTGGACTG 
CTGGAGGCCC GAGCCACCAA TGCACCCCCT ACCTCCAGCC CACTTCAGGT GTTCTTCAAC 
CCTAGGAGCA GCATCCTCTA TGAATCTACA CTGGTGGTGG AAGGCGTGCT GAGCGAGAAG 
CCCTGTGTCC TGAGGCTTCG GGGCCTAGGC TCTTACGATG AAAGATACGT GAAATTCCAT 
GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512215.1
CDS	70..5298
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTGSCSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVDVSFSLSLELLSYQKLSADQMLPGVDIQESENGERKMVFTQNLLLGFTNQTVQEVPLRAFVTLPAPQLSTSWVDFGTCLVNQQHTRQIYLMNLSCCLSYWTVLLGQEEPAREDHAFGVSPGSGLLEARATNAPPTSSPLQVFFNPRSSILYESTLVVEGVLSEKPCVLRLRGLGSYDERYVKFHEF

Target ORF information:

RefSeq Version	XM_006512152.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512152.4

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGGCAG CTGCAGTGGC GGCGGCGGCG GCACCAGGGC CGAAGAGCAG 
ATCATGTCTG TCATCCTGCA GAGGCACGAG GCAGTGCCCG CCGGCCACAT ATACCGTATC 
AGCCCCAAGC AGTTGGTCAT CCCAGCTGGA GACCACAGGA CCATCTACAT CTCCTTCACG 
CCCACGGTGC TCGACCCAGG GGTCATGCAC AAGGTGGGGT ATACTGGCTA TGCCCTGGGC 
TTCATGAGCT TGGATAAGGA GACAGACAGA CAGATTCCAG GCCGAATGCG ACGCCTTCAG 
GACTTTGCCG TGAGCCCCCT GAGACTGGAT CTGAATGGCC ACGTGAGACA CGCGCAGCTG 
CACGTCGAGC TGGACAGCAG TGGCTACTTG GAATTCTGGT GCCAGGCCAG CGACCTTATC 
CCCCAAAATC CCTGCAGTGG GGTGCTAAGT GACCGGATTA CCACCCGCCA TCTGAAACTG 
ACCAACACCA CTGAAATCCT GTATGACTTC CAGGCCCGGG TCTCCAGGCC CTTCTTCATT 
TCTCAAGGTG GGGCCAGCTG GGACAGCAGA GCCCCTCATC AGTGTGAAGA AGGGACGGCC 
TCCACAGGCC AGTGGCTGAT GATCCGTCCT CAGGACAACA TGCTGGTGGA TGTGTCCTTC 
TCACTGTCCC TGGAACTGCT GTCCTATCAG AAACTCTCGG CCGACCAGAT GTTGCCCGGA 
GTGGATATTC AGGAGAGCGA GAACGGAGAG AGAAAGATGG TGTTCACACA GAACCTACTC 
CTGGGCTTCA CCAACCAGAC TGTTCAGGAG GTGCCCCTGC GGGCCTTTGT GACCCTGCCA 
GCGCCACAGC TCTCCACCAG CTGGGTGGAC TTCGGGACCT GTCTCGTGAA CCAGCAGCAC 
ACCCGGCAGA TCTACCTGAT GAACCTCAGC TGCTGCCTGA GCTACTGGAC GGTGCTGTTG 
GGACAAGAGG AACCAGCCAG GGAGGATCAT GCGTTTGGGG TCTCCCCAGG CAGTGGACTG 
CTGGAGGCCC GAGCCACCAA TGCACCCCCT ACCTCCAGCC CACTTCAGGT GTTCTTCAAC 
CCTAGGAGCA GCATCCTCTA TGAATCTACA CTGGTGGTGG AAGGCGTGCT GAGCGAGAAG 
CCCTGTGTCC TGAGGCTTCG GGGCCTAGGC TCTTACGATG AAAGATACGT GAAATTCCAT 
GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( XM_006512153.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512216.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X2, mRNA.

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CloneID	OMu43441
Clone ID Related Accession (Same CDS sequence)	XM_006512153.3 , XM_006512153.4
Accession Version	XM_006512153.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512153.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GACAAGCAGG CCAAAGAATA AGAAGTGGAT GGAACACCTG 
CAGGTGCCGC AGAGAGCCCT GGACAGGCGG CTGCTCGCCA GCATGGAGAA CAGGAATCAC 
TTCCTGAAAA ACCCTCGGTT TTTCCCGCCT AACACACCAC ATGGAGGCAA GTCTCTCATC 
GTCCCTTTGA GCAAGCCAGA GAGAAGAGCA TCCCTGAATG CAGAGCCTGA GTGGAGCTGT 
ACAGATGCCC CCGTGTTCCT GGCGAAGCCA TCAGTTGGAT TTTTCACAGA TTACGAAATT 
GGACCCGTTT ACGAGATGGT GATCGCTCTA CAGAACACCA CCTCCACCAG CCGATTCTTG 
CGTGTCCTCC CACCGTCCTC TCCGTACTTC GCTCTGGGAC TTGGGATGTT CCCAGGAAAA 
GGCGGAATAG TGGCTCCCGG GATGACCTGC CAGTACATCG TCCAGTTCAT CCCGGACTGT 
CTTGGCGATT TTGATGATTT CATTTTGGTG GAGACACAGT CTCCCCACAC GCTGGTAATC 
CCCCTGCAGG CCCGGAGACC GCCGCCAGTG CTGACCTTGT CACCAGTGTT GGACTGCGGC 
CACTGCCTCA TTGGAGGGGT GAAGATAACC AAATTCATCT GCAAAAATGT GGGCTTCAGC 
GTCGGCAAAT TCTGTATCAT GCCCCAAAAG AGCTGGCCGC CACCGAGTTT CCGGGCTGTT 
GCAACTACTG GCTTTGTAGA GCAGCCTCCT TTTGGAGTAA TGCCTTCCGT GTTTGAGCTG 
GCCCCAGGGT ACGCCATACT GTTGGAGGTC TTGTTCCTCC CTACTGGCCT AGGACAGGCA 
GAAGAGACCT TCATCATCGT GTGTGACAAC TGTCAGATAA AGGAGGTGGT CGTCGTAGGG 
ACAGGGCAGC TGGTGGCTTT GGATCTGATT CATATTTCTG GTGGAAAAAA TGAGCCAGAC 
CCAGGAGAGC TGAAAGATTT AACAGCCCAG TACTTTATCC GGTTTGAGCC TGAAAACGTC 
CAGTCCACCG CAAAGAAGCA GCTCATCATC AGAAATGCTA CACACGTGGA GCTGGCCTTC 
CACTGGCAGA TCATGAAACC CAACCTGCAG CCTCTGATGC CTGGAGAGAC ACACAGCTCA 
GACAGCATCA AATGCCACCC TGACAGGGAG ACAGCCTTCT CCATCATCCC CGAGAAGGGC 
ATCCTCGAGT CCCACTCGGA CCAAGAGTTC ATCCTGAGTT TTTCACCTTA CAAGCTCAAG 
TGTTTCCACA GTGTACTGCA AATGGTTCTA GAAGCTGTGC CCGAGCCCTT GAGCTCAGGG 
TTGGAGAACC TGGGGGACTA CTCCTACTCA GTGGATGACA TGATCGTCCT GGAAATCGAG 
GTGAAAGGCT TGGCGGAACC CTTCCAGGTC CTTTTAGAGC CGTACGCCCT CATCATCCCT 
GGGGAGAACT ACATTGGCAT CACCGTGAAG AAAGATTTTA AGATGTGGAA CAACAGCAAG 
TCACCCATCA GATACATGTG GGGGAAGATC AGCCACTGCC ATATCATCGA AGTGGAGCCC 
TGCACTGGGG TGATAGAGCC CAATGAGGTC GGAGATTTTG AGTTAAACCT AACGGGTGGG 
GTGCCTGGCC CAACCAGCCA GGACCTGGAG TGTGAAATCA AAGACTCACC ATGCCCGGTG 
GTACTCCACA TTGAGGCCTC ATTTAAGGGA CCTGCCTTGA CCATTGATGT CCCAGCCCTT 
CAGTTTGGTC TGCTTCGCCT GGGAAAGAAG GCTTCCATCT CCATCCAGAT CCGGAATGTC 
AGCCAGCTGT CCGCCGTGTG GCATTTGAAG GAGAGCCCAG TCTGCCTGGC AGAGAGAGAT 
GAGGATGTGT CCCCCTTCGA CATCGAGCCT TTCTGCGGCC AGCTTCTCCC TCTGGGGGAG 
TGCAGAGTGA CCATCACCCT TGAGGCCTCC CAGTGTCAGC GGCTGCAGAC AGTCCTGGAG 
CTGGAAGTGG AGAACGGGTC CGGGAGCTAC CTTCCCGTGT ACGCTGAGGT GCAGCAGCCT 
CACGTGTACC TGAAGAACAG CCACTTGGAG TTCAGCAACC TCTACCTGGG CGTGTCCACA 
AAGTCAAGGA CCACGCTGGT CAATGGCACA CTCCTGCCCA CCCGGTTCCA CTGGGGAAAG 
CTTCTGGGAC CTCAAGCAGA TTTGTGCACA ATGACAGTCT CTCCCACACA GGGCCTGCTG 
GGCCCCAGTG AAGAGCTCCA ACTCACCTTG GAGTTGACGA CCCACACAGA GGAAAAGCTG 
ACCAATTTGG TTCTCCTTTG TCACGTGTCT GGCATGGAGA AGCCGCTGGT CCTGGGCATC 
TCTGGGAAGC CCCTGGGACT TCAGGTGACC ATCTCCATAG TGGAATCTGA CGGAAGCAGC 
ACCACCAGCA CTACACAGGA GGAGCTGCGC TTGGACTTCG GCTCGAAGGT GCCACTGAGG 
ACGCCCGTGA TTCGCCATAT CATCCTGACA AACTGCTCCC CGATACAGAC CCCATTCACC 
CTCACACTGG AGTACTTCGG GAGCTCCCAG GACAGCCTGT ACAAGAAGAC CAGCGTCCCT 
GACGTGCCTC CTGCCCTGCT GAAGACAGCA CGCATCCAGC AGCACGTGGC CAAGAAGGAA 
AGGCTGGACT TTGTGGAGAG CATGTTATCT CACAGGAAAG GAGCTGCCTT CCTTCCCCAC 
ATCTCCCAGG GCATGCTGGG AGCCTACCAG GAGCTATGTA TTGACATCAC AGGCTGTGCC 
AACATGTGGG GCGAGTACTG GGATGAGCTC CTCTGCGTGG TGGGAGACCT GCCGGTCACT 
GTCATCCCGG TATACATGGC CGTGGTGGGC TGCCCTATCA GCTCCCTGAG GAATACGTGC 
TACAGTGTGG CCCCATTCCA GAAGGAGCCT ATCACCAGGT TTGGGACCCA GATCTCTGGA 
GGAGATACAG TCACCAGATC TCTCCGTCTG TATAACTCCA GTCACTGTGA CATCCGCCTA 
GACTGGGAGA CCTACATCCC AGAAGAAAAG GCGGACCGGC TGCTGGAGCT GCTTGTGTTT 
TATGGGCCAC CCTTCCCACT GCGAGATCAA GACGGCAGTG AGATTGTGTG CCCTGAGACC 
TCGGAGGCCA GCTTACCAGG GTCTCCATGC CCCTCCAATG TCTCAGTGTC TAGCCACACT 
GTCCCCTCAA CGGGCAGCTG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTGGATGT GTCCTTCTCA 
CTGTCCCTGG AACTGCTGTC CTATCAGAAA CTCTCGGCCG ACCAGATGTT GCCCGGAGTG 
GATATTCAGG AGAGCGAGAA CGGAGAGAGA AAGATGGTGT TCACACAGAA CCTACTCCTG 
GGCTTCACCA ACCAGACTGT TCAGGAGGTG CCCCTGCGGG CCTTTGTGAC CCTGCCAGCG 
CCACAGCTCT CCACCAGCTG GGTGGACTTC GGGACCTGTC TCGTGAACCA GCAGCACACC 
CGGCAGATCT ACCTGATGAA CCTCAGCTGC TGCCTGAGCT ACTGGACGGT GCTGTTGGGA 
CAAGAGGAAC CAGCCAGGGA GGATCATGCG TTTGGGGTCT CCCCAGGCAG TGGACTGCTG 
GAGGCCCGAG CCACCAATGC ACCCCCTACC TCCAGCCCAC TTCAGGTGTT CTTCAACCCT 
AGGAGCAGCA TCCTCTATGA ATCTACACTG GTGGTGGAAG GCGTGCTGAG CGAGAAGCCC 
TGTGTCCTGA GGCTTCGGGG CCTAGGCTCT TACGATGAAA GATACGTGAA ATTCCATGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512216.1
CDS	70..5295
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTGSCSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVDVSFSLSLELLSYQKLSADQMLPGVDIQESENGERKMVFTQNLLLGFTNQTVQEVPLRAFVTLPAPQLSTSWVDFGTCLVNQQHTRQIYLMNLSCCLSYWTVLLGQEEPAREDHAFGVSPGSGLLEARATNAPPTSSPLQVFFNPRSSILYESTLVVEGVLSEKPCVLRLRGLGSYDERYVKFHEF

Target ORF information:

RefSeq Version	XM_006512153.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512153.4

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GACAAGCAGG CCAAAGAATA AGAAGTGGAT GGAACACCTG 
CAGGTGCCGC AGAGAGCCCT GGACAGGCGG CTGCTCGCCA GCATGGAGAA CAGGAATCAC 
TTCCTGAAAA ACCCTCGGTT TTTCCCGCCT AACACACCAC ATGGAGGCAA GTCTCTCATC 
GTCCCTTTGA GCAAGCCAGA GAGAAGAGCA TCCCTGAATG CAGAGCCTGA GTGGAGCTGT 
ACAGATGCCC CCGTGTTCCT GGCGAAGCCA TCAGTTGGAT TTTTCACAGA TTACGAAATT 
GGACCCGTTT ACGAGATGGT GATCGCTCTA CAGAACACCA CCTCCACCAG CCGATTCTTG 
CGTGTCCTCC CACCGTCCTC TCCGTACTTC GCTCTGGGAC TTGGGATGTT CCCAGGAAAA 
GGCGGAATAG TGGCTCCCGG GATGACCTGC CAGTACATCG TCCAGTTCAT CCCGGACTGT 
CTTGGCGATT TTGATGATTT CATTTTGGTG GAGACACAGT CTCCCCACAC GCTGGTAATC 
CCCCTGCAGG CCCGGAGACC GCCGCCAGTG CTGACCTTGT CACCAGTGTT GGACTGCGGC 
CACTGCCTCA TTGGAGGGGT GAAGATAACC AAATTCATCT GCAAAAATGT GGGCTTCAGC 
GTCGGCAAAT TCTGTATCAT GCCCCAAAAG AGCTGGCCGC CACCGAGTTT CCGGGCTGTT 
GCAACTACTG GCTTTGTAGA GCAGCCTCCT TTTGGAGTAA TGCCTTCCGT GTTTGAGCTG 
GCCCCAGGGT ACGCCATACT GTTGGAGGTC TTGTTCCTCC CTACTGGCCT AGGACAGGCA 
GAAGAGACCT TCATCATCGT GTGTGACAAC TGTCAGATAA AGGAGGTGGT CGTCGTAGGG 
ACAGGGCAGC TGGTGGCTTT GGATCTGATT CATATTTCTG GTGGAAAAAA TGAGCCAGAC 
CCAGGAGAGC TGAAAGATTT AACAGCCCAG TACTTTATCC GGTTTGAGCC TGAAAACGTC 
CAGTCCACCG CAAAGAAGCA GCTCATCATC AGAAATGCTA CACACGTGGA GCTGGCCTTC 
CACTGGCAGA TCATGAAACC CAACCTGCAG CCTCTGATGC CTGGAGAGAC ACACAGCTCA 
GACAGCATCA AATGCCACCC TGACAGGGAG ACAGCCTTCT CCATCATCCC CGAGAAGGGC 
ATCCTCGAGT CCCACTCGGA CCAAGAGTTC ATCCTGAGTT TTTCACCTTA CAAGCTCAAG 
TGTTTCCACA GTGTACTGCA AATGGTTCTA GAAGCTGTGC CCGAGCCCTT GAGCTCAGGG 
TTGGAGAACC TGGGGGACTA CTCCTACTCA GTGGATGACA TGATCGTCCT GGAAATCGAG 
GTGAAAGGCT TGGCGGAACC CTTCCAGGTC CTTTTAGAGC CGTACGCCCT CATCATCCCT 
GGGGAGAACT ACATTGGCAT CACCGTGAAG AAAGATTTTA AGATGTGGAA CAACAGCAAG 
TCACCCATCA GATACATGTG GGGGAAGATC AGCCACTGCC ATATCATCGA AGTGGAGCCC 
TGCACTGGGG TGATAGAGCC CAATGAGGTC GGAGATTTTG AGTTAAACCT AACGGGTGGG 
GTGCCTGGCC CAACCAGCCA GGACCTGGAG TGTGAAATCA AAGACTCACC ATGCCCGGTG 
GTACTCCACA TTGAGGCCTC ATTTAAGGGA CCTGCCTTGA CCATTGATGT CCCAGCCCTT 
CAGTTTGGTC TGCTTCGCCT GGGAAAGAAG GCTTCCATCT CCATCCAGAT CCGGAATGTC 
AGCCAGCTGT CCGCCGTGTG GCATTTGAAG GAGAGCCCAG TCTGCCTGGC AGAGAGAGAT 
GAGGATGTGT CCCCCTTCGA CATCGAGCCT TTCTGCGGCC AGCTTCTCCC TCTGGGGGAG 
TGCAGAGTGA CCATCACCCT TGAGGCCTCC CAGTGTCAGC GGCTGCAGAC AGTCCTGGAG 
CTGGAAGTGG AGAACGGGTC CGGGAGCTAC CTTCCCGTGT ACGCTGAGGT GCAGCAGCCT 
CACGTGTACC TGAAGAACAG CCACTTGGAG TTCAGCAACC TCTACCTGGG CGTGTCCACA 
AAGTCAAGGA CCACGCTGGT CAATGGCACA CTCCTGCCCA CCCGGTTCCA CTGGGGAAAG 
CTTCTGGGAC CTCAAGCAGA TTTGTGCACA ATGACAGTCT CTCCCACACA GGGCCTGCTG 
GGCCCCAGTG AAGAGCTCCA ACTCACCTTG GAGTTGACGA CCCACACAGA GGAAAAGCTG 
ACCAATTTGG TTCTCCTTTG TCACGTGTCT GGCATGGAGA AGCCGCTGGT CCTGGGCATC 
TCTGGGAAGC CCCTGGGACT TCAGGTGACC ATCTCCATAG TGGAATCTGA CGGAAGCAGC 
ACCACCAGCA CTACACAGGA GGAGCTGCGC TTGGACTTCG GCTCGAAGGT GCCACTGAGG 
ACGCCCGTGA TTCGCCATAT CATCCTGACA AACTGCTCCC CGATACAGAC CCCATTCACC 
CTCACACTGG AGTACTTCGG GAGCTCCCAG GACAGCCTGT ACAAGAAGAC CAGCGTCCCT 
GACGTGCCTC CTGCCCTGCT GAAGACAGCA CGCATCCAGC AGCACGTGGC CAAGAAGGAA 
AGGCTGGACT TTGTGGAGAG CATGTTATCT CACAGGAAAG GAGCTGCCTT CCTTCCCCAC 
ATCTCCCAGG GCATGCTGGG AGCCTACCAG GAGCTATGTA TTGACATCAC AGGCTGTGCC 
AACATGTGGG GCGAGTACTG GGATGAGCTC CTCTGCGTGG TGGGAGACCT GCCGGTCACT 
GTCATCCCGG TATACATGGC CGTGGTGGGC TGCCCTATCA GCTCCCTGAG GAATACGTGC 
TACAGTGTGG CCCCATTCCA GAAGGAGCCT ATCACCAGGT TTGGGACCCA GATCTCTGGA 
GGAGATACAG TCACCAGATC TCTCCGTCTG TATAACTCCA GTCACTGTGA CATCCGCCTA 
GACTGGGAGA CCTACATCCC AGAAGAAAAG GCGGACCGGC TGCTGGAGCT GCTTGTGTTT 
TATGGGCCAC CCTTCCCACT GCGAGATCAA GACGGCAGTG AGATTGTGTG CCCTGAGACC 
TCGGAGGCCA GCTTACCAGG GTCTCCATGC CCCTCCAATG TCTCAGTGTC TAGCCACACT 
GTCCCCTCAA CGGGCAGCTG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTGGATGT GTCCTTCTCA 
CTGTCCCTGG AACTGCTGTC CTATCAGAAA CTCTCGGCCG ACCAGATGTT GCCCGGAGTG 
GATATTCAGG AGAGCGAGAA CGGAGAGAGA AAGATGGTGT TCACACAGAA CCTACTCCTG 
GGCTTCACCA ACCAGACTGT TCAGGAGGTG CCCCTGCGGG CCTTTGTGAC CCTGCCAGCG 
CCACAGCTCT CCACCAGCTG GGTGGACTTC GGGACCTGTC TCGTGAACCA GCAGCACACC 
CGGCAGATCT ACCTGATGAA CCTCAGCTGC TGCCTGAGCT ACTGGACGGT GCTGTTGGGA 
CAAGAGGAAC CAGCCAGGGA GGATCATGCG TTTGGGGTCT CCCCAGGCAG TGGACTGCTG 
GAGGCCCGAG CCACCAATGC ACCCCCTACC TCCAGCCCAC TTCAGGTGTT CTTCAACCCT 
AGGAGCAGCA TCCTCTATGA ATCTACACTG GTGGTGGAAG GCGTGCTGAG CGAGAAGCCC 
TGTGTCCTGA GGCTTCGGGG CCTAGGCTCT TACGATGAAA GATACGTGAA ATTCCATGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( XM_006512154.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512217.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X3, mRNA.

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CloneID	OMu43442
Clone ID Related Accession (Same CDS sequence)	XM_006512154.4 , XM_006512154.3
Accession Version	XM_006512154.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512154.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
ACCACCAGCA CTACACAGGA GGAGCTGCGC TTGGACTTCG GCTCGAAGGT GCCACTGAGG 
ACGCCCGTGA TTCGCCATAT CATCCTGACA AACTGCTCCC CGATACAGAC CCCATTCACC 
CTCACACTGG AGTACTTCGG GAGCTCCCAG GACAGCCTGT ACAAGAAGAC CAGCGTCCCT 
GACGTGCCTC CTGCCCTGCT GAAGACAGCA CGCATCCAGC AGCACGTGGC CAAGAAGGAA 
AGGCTGGACT TTGTGGAGAG CATGTTATCT CACAGGAAAG GAGCTGCCTT CCTTCCCCAC 
ATCTCCCAGG GCATGCTGGG AGCCTACCAG GAGCTATGTA TTGACATCAC AGGCTGTGCC 
AACATGTGGG GCGAGTACTG GGATGAGCTC CTCTGCGTGG TGGGAGACCT GCCGGTCACT 
GTCATCCCGG TATACATGGC CGTGGTGGGC TGCCCTATCA GCTCCCTGAG GAATACGTGC 
TACAGTGTGG CCCCATTCCA GAAGGAGCCT ATCACCAGGT TTGGGACCCA GATCTCTGGA 
GGAGATACAG TCACCAGATC TCTCCGTCTG TATAACTCCA GTCACTGTGA CATCCGCCTA 
GACTGGGAGA CCTACATCCC AGAAGAAAAG GCGGACCGGC TGCTGGAGCT GCTTGTGTTT 
TATGGGCCAC CCTTCCCACT GCGAGATCAA GACGGCAGTG AGATTGTGTG CCCTGAGACC 
TCGGAGGCCA GCTTACCAGG GTCTCCATGC CCCTCCAATG TCTCAGTGTC TAGCCACACT 
GTCCCCTCAA CGGGCAGCTG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTGGATGT GTCCTTCTCA 
CTGTCCCTGG AACTGCTGTC CTATCAGAAA CTCTCGGCCG ACCAGATGTT GCCCGGAGTG 
GATATTCAGG AGAGCGAGAA CGGAGAGAGA AAGATGGTGT TCACACAGAA CCTACTCCTG 
GGCTTCACCA ACCAGACTGT TCAGGAGGTG CCCCTGCGGG CCTTTGTGAC CCTGCCAGCG 
CCACAGCTCT CCACCAGCTG GGTGGACTTC GGGACCTGTC TCGTGAACCA GCAGCACACC 
CGGCAGATCT ACCTGATGAA CCTCAGCTGC TGCCTGAGCT ACTGGACGGT GCTGTTGGGA 
CAAGAGGAAC CAGCCAGGGA GGATCATGCG TTTGGGGTCT CCCCAGGCAG TGGACTGCTG 
GAGGCCCGAG CCACCAATGC ACCCCCTACC TCCAGCCCAC TTCAGGTGTT CTTCAACCCT 
AGGAGCAGCA TCCTCTATGA ATCTACACTG GTGGTGGAAG GCGTGCTGAG CGAGAAGCCC 
TGTGTCCTGA GGCTTCGGGG CCTAGGCTCT TACGATGAAA GATACGTGAA ATTCCATGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512217.1
CDS	70..5295
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTGSCSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVDVSFSLSLELLSYQKLSADQMLPGVDIQESENGERKMVFTQNLLLGFTNQTVQEVPLRAFVTLPAPQLSTSWVDFGTCLVNQQHTRQIYLMNLSCCLSYWTVLLGQEEPAREDHAFGVSPGSGLLEARATNAPPTSSPLQVFFNPRSSILYESTLVVEGVLSEKPCVLRLRGLGSYDERYVKFHEF

Target ORF information:

RefSeq Version	XM_006512154.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512154.4

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
ACCACCAGCA CTACACAGGA GGAGCTGCGC TTGGACTTCG GCTCGAAGGT GCCACTGAGG 
ACGCCCGTGA TTCGCCATAT CATCCTGACA AACTGCTCCC CGATACAGAC CCCATTCACC 
CTCACACTGG AGTACTTCGG GAGCTCCCAG GACAGCCTGT ACAAGAAGAC CAGCGTCCCT 
GACGTGCCTC CTGCCCTGCT GAAGACAGCA CGCATCCAGC AGCACGTGGC CAAGAAGGAA 
AGGCTGGACT TTGTGGAGAG CATGTTATCT CACAGGAAAG GAGCTGCCTT CCTTCCCCAC 
ATCTCCCAGG GCATGCTGGG AGCCTACCAG GAGCTATGTA TTGACATCAC AGGCTGTGCC 
AACATGTGGG GCGAGTACTG GGATGAGCTC CTCTGCGTGG TGGGAGACCT GCCGGTCACT 
GTCATCCCGG TATACATGGC CGTGGTGGGC TGCCCTATCA GCTCCCTGAG GAATACGTGC 
TACAGTGTGG CCCCATTCCA GAAGGAGCCT ATCACCAGGT TTGGGACCCA GATCTCTGGA 
GGAGATACAG TCACCAGATC TCTCCGTCTG TATAACTCCA GTCACTGTGA CATCCGCCTA 
GACTGGGAGA CCTACATCCC AGAAGAAAAG GCGGACCGGC TGCTGGAGCT GCTTGTGTTT 
TATGGGCCAC CCTTCCCACT GCGAGATCAA GACGGCAGTG AGATTGTGTG CCCTGAGACC 
TCGGAGGCCA GCTTACCAGG GTCTCCATGC CCCTCCAATG TCTCAGTGTC TAGCCACACT 
GTCCCCTCAA CGGGCAGCTG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTGGATGT GTCCTTCTCA 
CTGTCCCTGG AACTGCTGTC CTATCAGAAA CTCTCGGCCG ACCAGATGTT GCCCGGAGTG 
GATATTCAGG AGAGCGAGAA CGGAGAGAGA AAGATGGTGT TCACACAGAA CCTACTCCTG 
GGCTTCACCA ACCAGACTGT TCAGGAGGTG CCCCTGCGGG CCTTTGTGAC CCTGCCAGCG 
CCACAGCTCT CCACCAGCTG GGTGGACTTC GGGACCTGTC TCGTGAACCA GCAGCACACC 
CGGCAGATCT ACCTGATGAA CCTCAGCTGC TGCCTGAGCT ACTGGACGGT GCTGTTGGGA 
CAAGAGGAAC CAGCCAGGGA GGATCATGCG TTTGGGGTCT CCCCAGGCAG TGGACTGCTG 
GAGGCCCGAG CCACCAATGC ACCCCCTACC TCCAGCCCAC TTCAGGTGTT CTTCAACCCT 
AGGAGCAGCA TCCTCTATGA ATCTACACTG GTGGTGGAAG GCGTGCTGAG CGAGAAGCCC 
TGTGTCCTGA GGCTTCGGGG CCTAGGCTCT TACGATGAAA GATACGTGAA ATTCCATGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( XM_006512155.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512218.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X4, mRNA.

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CloneID	OMu43443
Clone ID Related Accession (Same CDS sequence)	XM_006512155.3 , XM_006512155.4
Accession Version	XM_006512155.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512155.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGGCAG CTGCAGTGGC GGCGGCGGCG GCACCAGGGC CGAAGAGCAG 
ATCATGTCTG TCATCCTGCA GAGGCACGAG GCAGTGCCCG CCGGCCACAT ATACCGTATC 
AGCCCCAAGC AGTTGGTCAT CCCAGCTGGA GACCACAGGA CCATCTACAT CTCCTTCACG 
CCCACGGTGC TCGACCCAGG GGTCATGCAC AAGGTGGGGT ATACTGGCTA TGCCCTGGGC 
TTCATGAGCT TGGATAAGGA GACAGACAGA CAGATTCCAG GCCGAATGCG ACGCCTTCAG 
GACTTTGCCG TGAGCCCCCT GAGACTGGAT CTGAATGGCC ACGTGAGACA CGCGCAGCTG 
CACGTCGAGC TGGACAGCAG TGGCTACTTG GAATTCTGGT GCCAGGCCAG CGACCTTATC 
CCCCAAAATC CCTGCAGTGG GGTGCTAAGT GACCGGATTA CCACCCGCCA TCTGAAACTG 
ACCAACACCA CTGAAATCCT GTATGACTTC CAGGCCCGGG TCTCCAGGCC CTTCTTCATT 
TCTCAAGGTG GGGCCAGCTG GGACAGCAGA GCCCCTCATC AGTGTGAAGA AGGGACGGCC 
TCCACAGGCC AGTGGCTGAT GATCCGTCCT CAGGACAACA TGCTGAAACT CTCGGCCGAC 
CAGATGTTGC CCGGAGTGGA TATTCAGGAG AGCGAGAACG GAGAGAGAAA GATGGTGTTC 
ACACAGAACC TACTCCTGGG CTTCACCAAC CAGACTGTTC AGGAGGTGCC CCTGCGGGCC 
TTTGTGACCC TGCCAGCGCC ACAGCTCTCC ACCAGCTGGG TGGACTTCGG GACCTGTCTC 
GTGAACCAGC AGCACACCCG GCAGATCTAC CTGATGAACC TCAGCTGCTG CCTGAGCTAC 
TGGACGGTGC TGTTGGGACA AGAGGAACCA GCCAGGGAGG ATCATGCGTT TGGGGTCTCC 
CCAGGCAGTG GACTGCTGGA GGCCCGAGCC ACCAATGCAC CCCCTACCTC CAGCCCACTT 
CAGGTGTTCT TCAACCCTAG GAGCAGCATC CTCTATGAAT CTACACTGGT GGTGGAAGGC 
GTGCTGAGCG AGAAGCCCTG TGTCCTGAGG CTTCGGGGCC TAGGCTCTTA CGATGAAAGA 
TACGTGAAAT TCCATGAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512218.1
CDS	70..5253
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTGSCSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLKLSADQMLPGVDIQESENGERKMVFTQNLLLGFTNQTVQEVPLRAFVTLPAPQLSTSWVDFGTCLVNQQHTRQIYLMNLSCCLSYWTVLLGQEEPAREDHAFGVSPGSGLLEARATNAPPTSSPLQVFFNPRSSILYESTLVVEGVLSEKPCVLRLRGLGSYDERYVKFHEF

Target ORF information:

RefSeq Version	XM_006512155.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512155.4

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGGCAG CTGCAGTGGC GGCGGCGGCG GCACCAGGGC CGAAGAGCAG 
ATCATGTCTG TCATCCTGCA GAGGCACGAG GCAGTGCCCG CCGGCCACAT ATACCGTATC 
AGCCCCAAGC AGTTGGTCAT CCCAGCTGGA GACCACAGGA CCATCTACAT CTCCTTCACG 
CCCACGGTGC TCGACCCAGG GGTCATGCAC AAGGTGGGGT ATACTGGCTA TGCCCTGGGC 
TTCATGAGCT TGGATAAGGA GACAGACAGA CAGATTCCAG GCCGAATGCG ACGCCTTCAG 
GACTTTGCCG TGAGCCCCCT GAGACTGGAT CTGAATGGCC ACGTGAGACA CGCGCAGCTG 
CACGTCGAGC TGGACAGCAG TGGCTACTTG GAATTCTGGT GCCAGGCCAG CGACCTTATC 
CCCCAAAATC CCTGCAGTGG GGTGCTAAGT GACCGGATTA CCACCCGCCA TCTGAAACTG 
ACCAACACCA CTGAAATCCT GTATGACTTC CAGGCCCGGG TCTCCAGGCC CTTCTTCATT 
TCTCAAGGTG GGGCCAGCTG GGACAGCAGA GCCCCTCATC AGTGTGAAGA AGGGACGGCC 
TCCACAGGCC AGTGGCTGAT GATCCGTCCT CAGGACAACA TGCTGAAACT CTCGGCCGAC 
CAGATGTTGC CCGGAGTGGA TATTCAGGAG AGCGAGAACG GAGAGAGAAA GATGGTGTTC 
ACACAGAACC TACTCCTGGG CTTCACCAAC CAGACTGTTC AGGAGGTGCC CCTGCGGGCC 
TTTGTGACCC TGCCAGCGCC ACAGCTCTCC ACCAGCTGGG TGGACTTCGG GACCTGTCTC 
GTGAACCAGC AGCACACCCG GCAGATCTAC CTGATGAACC TCAGCTGCTG CCTGAGCTAC 
TGGACGGTGC TGTTGGGACA AGAGGAACCA GCCAGGGAGG ATCATGCGTT TGGGGTCTCC 
CCAGGCAGTG GACTGCTGGA GGCCCGAGCC ACCAATGCAC CCCCTACCTC CAGCCCACTT 
CAGGTGTTCT TCAACCCTAG GAGCAGCATC CTCTATGAAT CTACACTGGT GGTGGAAGGC 
GTGCTGAGCG AGAAGCCCTG TGTCCTGAGG CTTCGGGGCC TAGGCTCTTA CGATGAAAGA 
TACGTGAAAT TCCATGAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( XM_006512156.4 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512219.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X5, mRNA.

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CloneID	OMu43444
Clone ID Related Accession (Same CDS sequence)	XM_006512156.3 , XM_006512156.4
Accession Version	XM_006512156.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 8, 2019 this sequence version replaced XM_006512156.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGGCAG CTGCAGTGGC GGCGGCGGCG GCACCAGGGC CGAAGAGCAG 
ATCATGTCTG TCATCCTGCA GAGGCACGAG GCAGTGCCCG CCGGCCACAT ATACCGTATC 
AGCCCCAAGC AGTTGGTCAT CCCAGCTGGA GACCACAGGA CCATCTACAT CTCCTTCACG 
CCCACGGTGC TCGACCCAGG GGTCATGCAC AAGGTGGGGT ATACTGGCTA TGCCCTGGGC 
TTCATGAGCT TGGATAAGGA GACAGACAGA CAGATTCCAG GCCGAATGCG ACGCCTTCAG 
GACTTTGCCG TGAGCCCCCT GAGACTGGAT CTGAATGGCC ACGTGAGACA CGCGCAGCTG 
CACGTCGAGC TGGACAGCAG TGGCTACTTG GAATTCTGGT GCCAGGCCAG CGACCTTATC 
CCCCAAAATC CCTGCAGTGG GGTGCTAAGT GACCGGATTA CCACCCGCCA TCTGAAACTG 
ACCAACACCA CTGAAATCCT GTATGACTTC CAGGCCCGGG TCTCCAGGCC CTTCTTCATT 
TCTCAAGGTG GGGCCAGCTG GGACAGCAGA GCCCCTCATC AGTGTGAAGA AGGGACGGCC 
TCCACAGGCC AGTGGCTGAT GATCCGTCCT CAGGACAACA TGCTGGTGGA TGTGTCCTTC 
TCACTGTCCC TGGAACTGCT GTCCTATCAG AAACTCTCGG CCGACCAGAT GTTGCCCGGA 
GTGGATATTC AGGAGAGCGA GAACGGAGAG AGAAAGATGG TGTTCACACA GAACCTACTC 
CTGGGCTTCA CCAACCAGAC TGTTCAGGAG GTGCCCCTGC GGGCCTTTGT GACCCTGCCA 
GCGCCACAGC TCTCCACCAG CTGGGTGGAC TTCGGGACCT GTCTCGTGAA CCAGCAGCAC 
ACCCGGCAGA TCTACCTGAT GAACCTCAGC TGCTGCCTGA GCTACTGGAC GGTGCTGTTG 
GGACAAGAGG AACCAGCCAG GGAGGATCAT GCGTTTGGGG TCTCCCCAGG CAGTGGACTG 
CTGGAGGCCC GAGCCACCAA TGCACCCCCT ACCTCCAGCC CACTTCAGGT GTTCTTCAAC 
CCTAGGAGCA GCATCCTCTA TGAATCTACA CTGGTGGTGG AAGGCGTGCT GAGCGAGAAG 
CCCTGTGTCC TGAGGCTTCG GGGCCTAGGC TCTTACGATG AAAGATACGT GAAATTCCAT 
GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512219.1
CDS	70..5178
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTGSCSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVDVSFSLSLELLSYQKLSADQMLPGVDIQESENGERKMVFTQNLLLGFTNQTVQEVPLRAFVTLPAPQLSTSWVDFGTCLVNQQHTRQIYLMNLSCCLSYWTVLLGQEEPAREDHAFGVSPGSGLLEARATNAPPTSSPLQVFFNPRSSILYESTLVVEGVLSEKPCVLRLRGLGSYDERYVKFHEF

Target ORF information:

RefSeq Version	XM_006512156.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512156.4

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGGCAG CTGCAGTGGC GGCGGCGGCG GCACCAGGGC CGAAGAGCAG 
ATCATGTCTG TCATCCTGCA GAGGCACGAG GCAGTGCCCG CCGGCCACAT ATACCGTATC 
AGCCCCAAGC AGTTGGTCAT CCCAGCTGGA GACCACAGGA CCATCTACAT CTCCTTCACG 
CCCACGGTGC TCGACCCAGG GGTCATGCAC AAGGTGGGGT ATACTGGCTA TGCCCTGGGC 
TTCATGAGCT TGGATAAGGA GACAGACAGA CAGATTCCAG GCCGAATGCG ACGCCTTCAG 
GACTTTGCCG TGAGCCCCCT GAGACTGGAT CTGAATGGCC ACGTGAGACA CGCGCAGCTG 
CACGTCGAGC TGGACAGCAG TGGCTACTTG GAATTCTGGT GCCAGGCCAG CGACCTTATC 
CCCCAAAATC CCTGCAGTGG GGTGCTAAGT GACCGGATTA CCACCCGCCA TCTGAAACTG 
ACCAACACCA CTGAAATCCT GTATGACTTC CAGGCCCGGG TCTCCAGGCC CTTCTTCATT 
TCTCAAGGTG GGGCCAGCTG GGACAGCAGA GCCCCTCATC AGTGTGAAGA AGGGACGGCC 
TCCACAGGCC AGTGGCTGAT GATCCGTCCT CAGGACAACA TGCTGGTGGA TGTGTCCTTC 
TCACTGTCCC TGGAACTGCT GTCCTATCAG AAACTCTCGG CCGACCAGAT GTTGCCCGGA 
GTGGATATTC AGGAGAGCGA GAACGGAGAG AGAAAGATGG TGTTCACACA GAACCTACTC 
CTGGGCTTCA CCAACCAGAC TGTTCAGGAG GTGCCCCTGC GGGCCTTTGT GACCCTGCCA 
GCGCCACAGC TCTCCACCAG CTGGGTGGAC TTCGGGACCT GTCTCGTGAA CCAGCAGCAC 
ACCCGGCAGA TCTACCTGAT GAACCTCAGC TGCTGCCTGA GCTACTGGAC GGTGCTGTTG 
GGACAAGAGG AACCAGCCAG GGAGGATCAT GCGTTTGGGG TCTCCCCAGG CAGTGGACTG 
CTGGAGGCCC GAGCCACCAA TGCACCCCCT ACCTCCAGCC CACTTCAGGT GTTCTTCAAC 
CCTAGGAGCA GCATCCTCTA TGAATCTACA CTGGTGGTGG AAGGCGTGCT GAGCGAGAAG 
CCCTGTGTCC TGAGGCTTCG GGGCCTAGGC TCTTACGATG AAAGATACGT GAAATTCCAT 
GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( NM_177117.4 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796091.2 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant 1, mRNA.

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CloneID	OMu05953
Clone ID Related Accession (Same CDS sequence)	NM_177117.4 , NM_177117.5 , NM_177117.3
Accession Version	NM_177117.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC055818.9. On Feb 28, 2019 this sequence version replaced NM_177117.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849381 [ECO:0000350] ##Evidence-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGTGAG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTGGATGT GTCCTTCTCA 
CTGTCCCTGG AACTGCTGTC CTATCAGAAA CTCTCGGCCG ACCAGATGTT GCCCGGAGTG 
GATATTCAGG AGAGCGAGAA CGGAGAGAGA AAGATGGTGT TCACACAGAA CCTACTCCTG 
GGCTTCACCA ACCAGACTGT TCAGGAGGTG CCCCTGCGGG CCTTTGTGAC CCTGCCAGCG 
CCACAGCTCT CCACCAGCTG GGTGGACTTC GGGACCTGTC TCGTGAACCA GCAGCACACC 
CGGCAGATCT ACCTGATGAA CCTCAGCTGC TGCCTGAGCT ACTGGACGGT GCTGTTGGGA 
CAAGAGGAAC CAGCCAGGGA GGATCATGCG TTTGGGGTCT CCCCAGGCAG TGGACTGCTG 
GAGGCCCGAG CCACCAATGC ACCCCCTACC TCCAGCCCAC TTCAGGTGTT CTTCAACCCT 
AGGAGCAGCA TCCTCTATGA ATCTACACTG GTGGTGGAAG GCGTGCTGAG CGAGAAGCCC 
TGTGTCCTGA GGCTTCGGGG CCTAGGCTCT TACGATGAAA GATACGTGAA ATTCCATGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796091.2
CDS	7..5232
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTVSSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVDVSFSLSLELLSYQKLSADQMLPGVDIQESENGERKMVFTQNLLLGFTNQTVQEVPLRAFVTLPAPQLSTSWVDFGTCLVNQQHTRQIYLMNLSCCLSYWTVLLGQEEPAREDHAFGVSPGSGLLEARATNAPPTSSPLQVFFNPRSSILYESTLVVEGVLSEKPCVLRLRGLGSYDERYVKFHEF

Target ORF information:

RefSeq Version	NM_177117.4
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177117.4

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGTGAG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTGGATGT GTCCTTCTCA 
CTGTCCCTGG AACTGCTGTC CTATCAGAAA CTCTCGGCCG ACCAGATGTT GCCCGGAGTG 
GATATTCAGG AGAGCGAGAA CGGAGAGAGA AAGATGGTGT TCACACAGAA CCTACTCCTG 
GGCTTCACCA ACCAGACTGT TCAGGAGGTG CCCCTGCGGG CCTTTGTGAC CCTGCCAGCG 
CCACAGCTCT CCACCAGCTG GGTGGACTTC GGGACCTGTC TCGTGAACCA GCAGCACACC 
CGGCAGATCT ACCTGATGAA CCTCAGCTGC TGCCTGAGCT ACTGGACGGT GCTGTTGGGA 
CAAGAGGAAC CAGCCAGGGA GGATCATGCG TTTGGGGTCT CCCCAGGCAG TGGACTGCTG 
GAGGCCCGAG CCACCAATGC ACCCCCTACC TCCAGCCCAC TTCAGGTGTT CTTCAACCCT 
AGGAGCAGCA TCCTCTATGA ATCTACACTG GTGGTGGAAG GCGTGCTGAG CGAGAAGCCC 
TGTGTCCTGA GGCTTCGGGG CCTAGGCTCT TACGATGAAA GATACGTGAA ATTCCATGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( NM_177117.5 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796091.2 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu05953
Clone ID Related Accession (Same CDS sequence)	NM_177117.4 , NM_177117.5 , NM_177117.3
Accession Version	NM_177117.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-16
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC055818.9. On Jun 17, 2019 this sequence version replaced NM_177117.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849381 [ECO:0000350] ##Evidence-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGTGAG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTGGATGT GTCCTTCTCA 
CTGTCCCTGG AACTGCTGTC CTATCAGAAA CTCTCGGCCG ACCAGATGTT GCCCGGAGTG 
GATATTCAGG AGAGCGAGAA CGGAGAGAGA AAGATGGTGT TCACACAGAA CCTACTCCTG 
GGCTTCACCA ACCAGACTGT TCAGGAGGTG CCCCTGCGGG CCTTTGTGAC CCTGCCAGCG 
CCACAGCTCT CCACCAGCTG GGTGGACTTC GGGACCTGTC TCGTGAACCA GCAGCACACC 
CGGCAGATCT ACCTGATGAA CCTCAGCTGC TGCCTGAGCT ACTGGACGGT GCTGTTGGGA 
CAAGAGGAAC CAGCCAGGGA GGATCATGCG TTTGGGGTCT CCCCAGGCAG TGGACTGCTG 
GAGGCCCGAG CCACCAATGC ACCCCCTACC TCCAGCCCAC TTCAGGTGTT CTTCAACCCT 
AGGAGCAGCA TCCTCTATGA ATCTACACTG GTGGTGGAAG GCGTGCTGAG CGAGAAGCCC 
TGTGTCCTGA GGCTTCGGGG CCTAGGCTCT TACGATGAAA GATACGTGAA ATTCCATGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796091.2
CDS	7..5232
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTVSSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVDVSFSLSLELLSYQKLSADQMLPGVDIQESENGERKMVFTQNLLLGFTNQTVQEVPLRAFVTLPAPQLSTSWVDFGTCLVNQQHTRQIYLMNLSCCLSYWTVLLGQEEPAREDHAFGVSPGSGLLEARATNAPPTSSPLQVFFNPRSSILYESTLVVEGVLSEKPCVLRLRGLGSYDERYVKFHEF

Target ORF information:

RefSeq Version	NM_177117.5
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177117.5

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGTGAG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTGGATGT GTCCTTCTCA 
CTGTCCCTGG AACTGCTGTC CTATCAGAAA CTCTCGGCCG ACCAGATGTT GCCCGGAGTG 
GATATTCAGG AGAGCGAGAA CGGAGAGAGA AAGATGGTGT TCACACAGAA CCTACTCCTG 
GGCTTCACCA ACCAGACTGT TCAGGAGGTG CCCCTGCGGG CCTTTGTGAC CCTGCCAGCG 
CCACAGCTCT CCACCAGCTG GGTGGACTTC GGGACCTGTC TCGTGAACCA GCAGCACACC 
CGGCAGATCT ACCTGATGAA CCTCAGCTGC TGCCTGAGCT ACTGGACGGT GCTGTTGGGA 
CAAGAGGAAC CAGCCAGGGA GGATCATGCG TTTGGGGTCT CCCCAGGCAG TGGACTGCTG 
GAGGCCCGAG CCACCAATGC ACCCCCTACC TCCAGCCCAC TTCAGGTGTT CTTCAACCCT 
AGGAGCAGCA TCCTCTATGA ATCTACACTG GTGGTGGAAG GCGTGCTGAG CGAGAAGCCC 
TGTGTCCTGA GGCTTCGGGG CCTAGGCTCT TACGATGAAA GATACGTGAA ATTCCATGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( NM_177117.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_796091.2 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), mRNA.

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CloneID	OMu05953
Clone ID Related Accession (Same CDS sequence)	NM_177117.4 , NM_177117.5 , NM_177117.3
Accession Version	NM_177117.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-23
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC055818.9. On or before Apr 5, 2009 this sequence version replaced XM_916519.2, XM_486268.3, NM_177117.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849381 [ECO:0000350] ##Evidence-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGTGAG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTGGATGT GTCCTTCTCA 
CTGTCCCTGG AACTGCTGTC CTATCAGAAA CTCTCGGCCG ACCAGATGTT GCCCGGAGTG 
GATATTCAGG AGAGCGAGAA CGGAGAGAGA AAGATGGTGT TCACACAGAA CCTACTCCTG 
GGCTTCACCA ACCAGACTGT TCAGGAGGTG CCCCTGCGGG CCTTTGTGAC CCTGCCAGCG 
CCACAGCTCT CCACCAGCTG GGTGGACTTC GGGACCTGTC TCGTGAACCA GCAGCACACC 
CGGCAGATCT ACCTGATGAA CCTCAGCTGC TGCCTGAGCT ACTGGACGGT GCTGTTGGGA 
CAAGAGGAAC CAGCCAGGGA GGATCATGCG TTTGGGGTCT CCCCAGGCAG TGGACTGCTG 
GAGGCCCGAG CCACCAATGC ACCCCCTACC TCCAGCCCAC TTCAGGTGTT CTTCAACCCT 
AGGAGCAGCA TCCTCTATGA ATCTACACTG GTGGTGGAAG GCGTGCTGAG CGAGAAGCCC 
TGTGTCCTGA GGCTTCGGGG CCTAGGCTCT TACGATGAAA GATACGTGAA ATTCCATGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_796091.2
CDS	38..5263
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTVSSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVDVSFSLSLELLSYQKLSADQMLPGVDIQESENGERKMVFTQNLLLGFTNQTVQEVPLRAFVTLPAPQLSTSWVDFGTCLVNQQHTRQIYLMNLSCCLSYWTVLLGQEEPAREDHAFGVSPGSGLLEARATNAPPTSSPLQVFFNPRSSILYESTLVVEGVLSEKPCVLRLRGLGSYDERYVKFHEF

Target ORF information:

RefSeq Version	NM_177117.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_177117.3

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGTGAG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTGGATGT GTCCTTCTCA 
CTGTCCCTGG AACTGCTGTC CTATCAGAAA CTCTCGGCCG ACCAGATGTT GCCCGGAGTG 
GATATTCAGG AGAGCGAGAA CGGAGAGAGA AAGATGGTGT TCACACAGAA CCTACTCCTG 
GGCTTCACCA ACCAGACTGT TCAGGAGGTG CCCCTGCGGG CCTTTGTGAC CCTGCCAGCG 
CCACAGCTCT CCACCAGCTG GGTGGACTTC GGGACCTGTC TCGTGAACCA GCAGCACACC 
CGGCAGATCT ACCTGATGAA CCTCAGCTGC TGCCTGAGCT ACTGGACGGT GCTGTTGGGA 
CAAGAGGAAC CAGCCAGGGA GGATCATGCG TTTGGGGTCT CCCCAGGCAG TGGACTGCTG 
GAGGCCCGAG CCACCAATGC ACCCCCTACC TCCAGCCCAC TTCAGGTGTT CTTCAACCCT 
AGGAGCAGCA TCCTCTATGA ATCTACACTG GTGGTGGAAG GCGTGCTGAG CGAGAAGCCC 
TGTGTCCTGA GGCTTCGGGG CCTAGGCTCT TACGATGAAA GATACGTGAA ATTCCATGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( NM_001368821.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001355750.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant 3, mRNA.

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CloneID	OMu43445
Clone ID Related Accession (Same CDS sequence)	NM_001368821.1 , XM_006512157.3
Accession Version	NM_001368821.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-27
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC055818.9. On Feb 28, 2019 this sequence version replaced XM_006512157.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC145277.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849381 [ECO:0000348] ##Evidence-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GACAAGCAGG CCAAAGAATA AGAAGTGGAT GGAACACCTG 
CAGGTGCCGC AGAGAGCCCT GGACAGGCGG CTGCTCGCCA GCATGGAGAA CAGGAATCAC 
TTCCTGAAAA ACCCTCGGTT TTTCCCGCCT AACACACCAC ATGGAGGCAA GTCTCTCATC 
GTCCCTTTGA GCAAGCCAGA GAGAAGAGCA TCCCTGAATG CAGAGCCTGA GTGGAGCTGT 
ACAGATGCCC CCGTGTTCCT GGCGAAGCCA TCAGTTGGAT TTTTCACAGA TTACGAAATT 
GGACCCGTTT ACGAGATGGT GATCGCTCTA CAGAACACCA CCTCCACCAG CCGATTCTTG 
CGTGTCCTCC CACCGTCCTC TCCGTACTTC GCTCTGGGAC TTGGGATGTT CCCAGGAAAA 
GGCGGAATAG TGGCTCCCGG GATGACCTGC CAGTACATCG TCCAGTTCAT CCCGGACTGT 
CTTGGCGATT TTGATGATTT CATTTTGGTG GAGACACAGT CTCCCCACAC GCTGGTAATC 
CCCCTGCAGG CCCGGAGACC GCCGCCAGTG CTGACCTTGT CACCAGTGTT GGACTGCGGC 
CACTGCCTCA TTGGAGGGGT GAAGATAACC AAATTCATCT GCAAAAATGT GGGCTTCAGC 
GTCGGCAAAT TCTGTATCAT GCCCCAAAAG AGCTGGCCGC CACCGAGTTT CCGGGCTGTT 
GCAACTACTG GCTTTGTAGA GCAGCCTCCT TTTGGAGTAA TGCCTTCCGT GTTTGAGCTG 
GCCCCAGGGT ACGCCATACT GTTGGAGGTC TTGTTCCTCC CTACTGGCCT AGGACAGGCA 
GAAGAGACCT TCATCATCGT GTGTGACAAC TGTCAGATAA AGGAGGTGGT CGTCGTAGGG 
ACAGGGCAGC TGGTGGCTTT GGATCTGATT CATATTTCTG GTGGAAAAAA TGAGCCAGAC 
CCAGGAGAGC TGAAAGATTT AACAGCCCAG TACTTTATCC GGTTTGAGCC TGAAAACGTC 
CAGTCCACCG CAAAGAAGCA GCTCATCATC AGAAATGCTA CACACGTGGA GCTGGCCTTC 
CACTGGCAGA TCATGAAACC CAACCTGCAG CCTCTGATGC CTGGAGAGAC ACACAGCTCA 
GACAGCATCA AATGCCACCC TGACAGGGAG ACAGCCTTCT CCATCATCCC CGAGAAGGGC 
ATCCTCGAGT CCCACTCGGA CCAAGAGTTC ATCCTGAGTT TTTCACCTTA CAAGCTCAAG 
TGTTTCCACA GTGTACTGCA AATGGTTCTA GAAGCTGTGC CCGAGCCCTT GAGCTCAGGG 
TTGGAGAACC TGGGGGACTA CTCCTACTCA GTGGATGACA TGATCGTCCT GGAAATCGAG 
GTGAAAGGCT TGGCGGAACC CTTCCAGGTC CTTTTAGAGC CGTACGCCCT CATCATCCCT 
GGGGAGAACT ACATTGGCAT CACCGTGAAG AAAGATTTTA AGATGTGGAA CAACAGCAAG 
TCACCCATCA GATACATGTG GGGGAAGATC AGCCACTGCC ATATCATCGA AGTGGAGCCC 
TGCACTGGGG TGATAGAGCC CAATGAGGTC GGAGATTTTG AGTTAAACCT AACGGGTGGG 
GTGCCTGGCC CAACCAGCCA GGACCTGGAG TGTGAAATCA AAGACTCACC ATGCCCGGTG 
GTACTCCACA TTGAGGCCTC ATTTAAGGGA CCTGCCTTGA CCATTGATGT CCCAGCCCTT 
CAGTTTGGTC TGCTTCGCCT GGGAAAGAAG GCTTCCATCT CCATCCAGAT CCGGAATGTC 
AGCCAGCTGT CCGCCGTGTG GCATTTGAAG GAGAGCCCAG TCTGCCTGGC AGAGAGAGAT 
GAGGATGTGT CCCCCTTCGA CATCGAGCCT TTCTGCGGCC AGCTTCTCCC TCTGGGGGAG 
TGCAGAGTGA CCATCACCCT TGAGGCCTCC CAGTGTCAGC GGCTGCAGAC AGTCCTGGAG 
CTGGAAGTGG AGAACGGGTC CGGGAGCTAC CTTCCCGTGT ACGCTGAGGT GCAGCAGCCT 
CACGTGTACC TGAAGAACAG CCACTTGGAG TTCAGCAACC TCTACCTGGG CGTGTCCACA 
AAGTCAAGGA CCACGCTGGT CAATGGCACA CTCCTGCCCA CCCGGTTCCA CTGGGGAAAG 
CTTCTGGGAC CTCAAGCAGA TTTGTGCACA ATGACAGTCT CTCCCACACA GGGCCTGCTG 
GGCCCCAGTG AAGAGCTCCA ACTCACCTTG GAGTTGACGA CCCACACAGA GGAAAAGCTG 
ACCAATTTGG TTCTCCTTTG TCACGTGTCT GGCATGGAGA AGCCGCTGGT CCTGGGCATC 
TCTGGGAAGC CCCTGGGACT TCAGGTGACC ATCTCCATAG TGGAATCTGA CGGAAGCACC 
ACCAGCACTA CACAGGAGGA GCTGCGCTTG GACTTCGGCT CGAAGGTGCC ACTGAGGACG 
CCCGTGATTC GCCATATCAT CCTGACAAAC TGCTCCCCGA TACAGACCCC ATTCACCCTC 
ACACTGGAGT ACTTCGGGAG CTCCCAGGAC AGCCTGTACA AGAAGACCAG CGTCCCTGAC 
GTGCCTCCTG CCCTGCTGAA GACAGCACGC ATCCAGCAGC ACGTGGCCAA GAAGGAAAGG 
CTGGACTTTG TGGAGAGCAT GTTATCTCAC AGGAAAGGAG CTGCCTTCCT TCCCCACATC 
TCCCAGGGCA TGCTGGGAGC CTACCAGGAG CTATGTATTG ACATCACAGG CTGTGCCAAC 
ATGTGGGGCG AGTACTGGGA TGAGCTCCTC TGCGTGGTGG GAGACCTGCC GGTCACTGTC 
ATCCCGGTAT ACATGGCCGT GGTGGGCTGC CCTATCAGCT CCCTGAGGAA TACGTGCTAC 
AGTGTGGCCC CATTCCAGAA GGAGCCTATC ACCAGGTTTG GGACCCAGAT CTCTGGAGGA 
GATACAGTCA CCAGATCTCT CCGTCTGTAT AACTCCAGTC ACTGTGACAT CCGCCTAGAC 
TGGGAGACCT ACATCCCAGA AGAAAAGGCG GACCGGCTGC TGGAGCTGCT TGTGTTTTAT 
GGGCCACCCT TCCCACTGCG AGATCAAGAC GGCAGTGAGA TTGTGTGCCC TGAGACCTCG 
GAGGCCAGCT TACCAGGGTC TCCATGCCCC TCCAATGTCT CAGTGTCTAG CCACACTGTC 
CCCTCAACGG GCAGCTGCAG TGGCGGCGGC GGCGGCACCA GGGCCGAAGA GCAGATCATG 
TCTGTCATCC TGCAGAGGCA CGAGGCAGTG CCCGCCGGCC ACATATACCG TATCAGCCCC 
AAGCAGTTGG TCATCCCAGC TGGAGACCAC AGGACCATCT ACATCTCCTT CACGCCCACG 
GTGCTCGACC CAGGGGTCAT GCACAAGGTG GGGTATACTG GCTATGCCCT GGGCTTCATG 
AGCTTGGATA AGGAGACAGA CAGACAGATT CCAGGCCGAA TGCGACGCCT TCAGGACTTT 
GCCGTGAGCC CCCTGAGACT GGATCTGAAT GGCCACGTGA GACACGCGCA GCTGCACGTC 
GAGCTGGACA GCAGTGGCTA CTTGGAATTC TGGTGCCAGG CCAGCGACCT TATCCCCCAA 
AATCCCTGCA GTGGGGTGCT AAGTGACCGG ATTACCACCC GCCATCTGAA ACTGACCAAC 
ACCACTGAAA TCCTGTATGA CTTCCAGGCC CGGGTCTCCA GGCCCTTCTT CATTTCTCAA 
GGTGGGGCCA GCTGGGACAG CAGAGCCCCT CATCAGTGTG AAGAAGGGAC GGCCTCCACA 
GGCCAGTGGC TGATGATCCG TCCTCAGGAC AACATGCTGG TCAGTGCAGG GGTGGAGCGT 
CTCCCCCACC ACCCCCGCCT CTATAGGCAA CACACAGGCA CATCCTGCCC ACATCTGGGA 
ACGAGGTACA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001355750.1
CDS	7..4761
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTGSCSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVSAGVERLPHHPRLYRQHTGTSCPHLGTRYK

Target ORF information:

RefSeq Version	NM_001368821.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001368821.1

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GACAAGCAGG CCAAAGAATA AGAAGTGGAT GGAACACCTG 
CAGGTGCCGC AGAGAGCCCT GGACAGGCGG CTGCTCGCCA GCATGGAGAA CAGGAATCAC 
TTCCTGAAAA ACCCTCGGTT TTTCCCGCCT AACACACCAC ATGGAGGCAA GTCTCTCATC 
GTCCCTTTGA GCAAGCCAGA GAGAAGAGCA TCCCTGAATG CAGAGCCTGA GTGGAGCTGT 
ACAGATGCCC CCGTGTTCCT GGCGAAGCCA TCAGTTGGAT TTTTCACAGA TTACGAAATT 
GGACCCGTTT ACGAGATGGT GATCGCTCTA CAGAACACCA CCTCCACCAG CCGATTCTTG 
CGTGTCCTCC CACCGTCCTC TCCGTACTTC GCTCTGGGAC TTGGGATGTT CCCAGGAAAA 
GGCGGAATAG TGGCTCCCGG GATGACCTGC CAGTACATCG TCCAGTTCAT CCCGGACTGT 
CTTGGCGATT TTGATGATTT CATTTTGGTG GAGACACAGT CTCCCCACAC GCTGGTAATC 
CCCCTGCAGG CCCGGAGACC GCCGCCAGTG CTGACCTTGT CACCAGTGTT GGACTGCGGC 
CACTGCCTCA TTGGAGGGGT GAAGATAACC AAATTCATCT GCAAAAATGT GGGCTTCAGC 
GTCGGCAAAT TCTGTATCAT GCCCCAAAAG AGCTGGCCGC CACCGAGTTT CCGGGCTGTT 
GCAACTACTG GCTTTGTAGA GCAGCCTCCT TTTGGAGTAA TGCCTTCCGT GTTTGAGCTG 
GCCCCAGGGT ACGCCATACT GTTGGAGGTC TTGTTCCTCC CTACTGGCCT AGGACAGGCA 
GAAGAGACCT TCATCATCGT GTGTGACAAC TGTCAGATAA AGGAGGTGGT CGTCGTAGGG 
ACAGGGCAGC TGGTGGCTTT GGATCTGATT CATATTTCTG GTGGAAAAAA TGAGCCAGAC 
CCAGGAGAGC TGAAAGATTT AACAGCCCAG TACTTTATCC GGTTTGAGCC TGAAAACGTC 
CAGTCCACCG CAAAGAAGCA GCTCATCATC AGAAATGCTA CACACGTGGA GCTGGCCTTC 
CACTGGCAGA TCATGAAACC CAACCTGCAG CCTCTGATGC CTGGAGAGAC ACACAGCTCA 
GACAGCATCA AATGCCACCC TGACAGGGAG ACAGCCTTCT CCATCATCCC CGAGAAGGGC 
ATCCTCGAGT CCCACTCGGA CCAAGAGTTC ATCCTGAGTT TTTCACCTTA CAAGCTCAAG 
TGTTTCCACA GTGTACTGCA AATGGTTCTA GAAGCTGTGC CCGAGCCCTT GAGCTCAGGG 
TTGGAGAACC TGGGGGACTA CTCCTACTCA GTGGATGACA TGATCGTCCT GGAAATCGAG 
GTGAAAGGCT TGGCGGAACC CTTCCAGGTC CTTTTAGAGC CGTACGCCCT CATCATCCCT 
GGGGAGAACT ACATTGGCAT CACCGTGAAG AAAGATTTTA AGATGTGGAA CAACAGCAAG 
TCACCCATCA GATACATGTG GGGGAAGATC AGCCACTGCC ATATCATCGA AGTGGAGCCC 
TGCACTGGGG TGATAGAGCC CAATGAGGTC GGAGATTTTG AGTTAAACCT AACGGGTGGG 
GTGCCTGGCC CAACCAGCCA GGACCTGGAG TGTGAAATCA AAGACTCACC ATGCCCGGTG 
GTACTCCACA TTGAGGCCTC ATTTAAGGGA CCTGCCTTGA CCATTGATGT CCCAGCCCTT 
CAGTTTGGTC TGCTTCGCCT GGGAAAGAAG GCTTCCATCT CCATCCAGAT CCGGAATGTC 
AGCCAGCTGT CCGCCGTGTG GCATTTGAAG GAGAGCCCAG TCTGCCTGGC AGAGAGAGAT 
GAGGATGTGT CCCCCTTCGA CATCGAGCCT TTCTGCGGCC AGCTTCTCCC TCTGGGGGAG 
TGCAGAGTGA CCATCACCCT TGAGGCCTCC CAGTGTCAGC GGCTGCAGAC AGTCCTGGAG 
CTGGAAGTGG AGAACGGGTC CGGGAGCTAC CTTCCCGTGT ACGCTGAGGT GCAGCAGCCT 
CACGTGTACC TGAAGAACAG CCACTTGGAG TTCAGCAACC TCTACCTGGG CGTGTCCACA 
AAGTCAAGGA CCACGCTGGT CAATGGCACA CTCCTGCCCA CCCGGTTCCA CTGGGGAAAG 
CTTCTGGGAC CTCAAGCAGA TTTGTGCACA ATGACAGTCT CTCCCACACA GGGCCTGCTG 
GGCCCCAGTG AAGAGCTCCA ACTCACCTTG GAGTTGACGA CCCACACAGA GGAAAAGCTG 
ACCAATTTGG TTCTCCTTTG TCACGTGTCT GGCATGGAGA AGCCGCTGGT CCTGGGCATC 
TCTGGGAAGC CCCTGGGACT TCAGGTGACC ATCTCCATAG TGGAATCTGA CGGAAGCACC 
ACCAGCACTA CACAGGAGGA GCTGCGCTTG GACTTCGGCT CGAAGGTGCC ACTGAGGACG 
CCCGTGATTC GCCATATCAT CCTGACAAAC TGCTCCCCGA TACAGACCCC ATTCACCCTC 
ACACTGGAGT ACTTCGGGAG CTCCCAGGAC AGCCTGTACA AGAAGACCAG CGTCCCTGAC 
GTGCCTCCTG CCCTGCTGAA GACAGCACGC ATCCAGCAGC ACGTGGCCAA GAAGGAAAGG 
CTGGACTTTG TGGAGAGCAT GTTATCTCAC AGGAAAGGAG CTGCCTTCCT TCCCCACATC 
TCCCAGGGCA TGCTGGGAGC CTACCAGGAG CTATGTATTG ACATCACAGG CTGTGCCAAC 
ATGTGGGGCG AGTACTGGGA TGAGCTCCTC TGCGTGGTGG GAGACCTGCC GGTCACTGTC 
ATCCCGGTAT ACATGGCCGT GGTGGGCTGC CCTATCAGCT CCCTGAGGAA TACGTGCTAC 
AGTGTGGCCC CATTCCAGAA GGAGCCTATC ACCAGGTTTG GGACCCAGAT CTCTGGAGGA 
GATACAGTCA CCAGATCTCT CCGTCTGTAT AACTCCAGTC ACTGTGACAT CCGCCTAGAC 
TGGGAGACCT ACATCCCAGA AGAAAAGGCG GACCGGCTGC TGGAGCTGCT TGTGTTTTAT 
GGGCCACCCT TCCCACTGCG AGATCAAGAC GGCAGTGAGA TTGTGTGCCC TGAGACCTCG 
GAGGCCAGCT TACCAGGGTC TCCATGCCCC TCCAATGTCT CAGTGTCTAG CCACACTGTC 
CCCTCAACGG GCAGCTGCAG TGGCGGCGGC GGCGGCACCA GGGCCGAAGA GCAGATCATG 
TCTGTCATCC TGCAGAGGCA CGAGGCAGTG CCCGCCGGCC ACATATACCG TATCAGCCCC 
AAGCAGTTGG TCATCCCAGC TGGAGACCAC AGGACCATCT ACATCTCCTT CACGCCCACG 
GTGCTCGACC CAGGGGTCAT GCACAAGGTG GGGTATACTG GCTATGCCCT GGGCTTCATG 
AGCTTGGATA AGGAGACAGA CAGACAGATT CCAGGCCGAA TGCGACGCCT TCAGGACTTT 
GCCGTGAGCC CCCTGAGACT GGATCTGAAT GGCCACGTGA GACACGCGCA GCTGCACGTC 
GAGCTGGACA GCAGTGGCTA CTTGGAATTC TGGTGCCAGG CCAGCGACCT TATCCCCCAA 
AATCCCTGCA GTGGGGTGCT AAGTGACCGG ATTACCACCC GCCATCTGAA ACTGACCAAC 
ACCACTGAAA TCCTGTATGA CTTCCAGGCC CGGGTCTCCA GGCCCTTCTT CATTTCTCAA 
GGTGGGGCCA GCTGGGACAG CAGAGCCCCT CATCAGTGTG AAGAAGGGAC GGCCTCCACA 
GGCCAGTGGC TGATGATCCG TCCTCAGGAC AACATGCTGG TCAGTGCAGG GGTGGAGCGT 
CTCCCCCACC ACCCCCGCCT CTATAGGCAA CACACAGGCA CATCCTGCCC ACATCTGGGA 
ACGAGGTACA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( XM_006512152.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512215.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X1, mRNA.

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CloneID	OMu43440
Clone ID Related Accession (Same CDS sequence)	XM_006512152.4 , XM_006512152.3
Accession Version	XM_006512152.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5229bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512152.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGGCAG CTGCAGTGGC GGCGGCGGCG GCACCAGGGC CGAAGAGCAG 
ATCATGTCTG TCATCCTGCA GAGGCACGAG GCAGTGCCCG CCGGCCACAT ATACCGTATC 
AGCCCCAAGC AGTTGGTCAT CCCAGCTGGA GACCACAGGA CCATCTACAT CTCCTTCACG 
CCCACGGTGC TCGACCCAGG GGTCATGCAC AAGGTGGGGT ATACTGGCTA TGCCCTGGGC 
TTCATGAGCT TGGATAAGGA GACAGACAGA CAGATTCCAG GCCGAATGCG ACGCCTTCAG 
GACTTTGCCG TGAGCCCCCT GAGACTGGAT CTGAATGGCC ACGTGAGACA CGCGCAGCTG 
CACGTCGAGC TGGACAGCAG TGGCTACTTG GAATTCTGGT GCCAGGCCAG CGACCTTATC 
CCCCAAAATC CCTGCAGTGG GGTGCTAAGT GACCGGATTA CCACCCGCCA TCTGAAACTG 
ACCAACACCA CTGAAATCCT GTATGACTTC CAGGCCCGGG TCTCCAGGCC CTTCTTCATT 
TCTCAAGGTG GGGCCAGCTG GGACAGCAGA GCCCCTCATC AGTGTGAAGA AGGGACGGCC 
TCCACAGGCC AGTGGCTGAT GATCCGTCCT CAGGACAACA TGCTGGTGGA TGTGTCCTTC 
TCACTGTCCC TGGAACTGCT GTCCTATCAG AAACTCTCGG CCGACCAGAT GTTGCCCGGA 
GTGGATATTC AGGAGAGCGA GAACGGAGAG AGAAAGATGG TGTTCACACA GAACCTACTC 
CTGGGCTTCA CCAACCAGAC TGTTCAGGAG GTGCCCCTGC GGGCCTTTGT GACCCTGCCA 
GCGCCACAGC TCTCCACCAG CTGGGTGGAC TTCGGGACCT GTCTCGTGAA CCAGCAGCAC 
ACCCGGCAGA TCTACCTGAT GAACCTCAGC TGCTGCCTGA GCTACTGGAC GGTGCTGTTG 
GGACAAGAGG AACCAGCCAG GGAGGATCAT GCGTTTGGGG TCTCCCCAGG CAGTGGACTG 
CTGGAGGCCC GAGCCACCAA TGCACCCCCT ACCTCCAGCC CACTTCAGGT GTTCTTCAAC 
CCTAGGAGCA GCATCCTCTA TGAATCTACA CTGGTGGTGG AAGGCGTGCT GAGCGAGAAG 
CCCTGTGTCC TGAGGCTTCG GGGCCTAGGC TCTTACGATG AAAGATACGT GAAATTCCAT 
GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512215.1
CDS	979..6207
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTGSCSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVDVSFSLSLELLSYQKLSADQMLPGVDIQESENGERKMVFTQNLLLGFTNQTVQEVPLRAFVTLPAPQLSTSWVDFGTCLVNQQHTRQIYLMNLSCCLSYWTVLLGQEEPAREDHAFGVSPGSGLLEARATNAPPTSSPLQVFFNPRSSILYESTLVVEGVLSEKPCVLRLRGLGSYDERYVKFHEF

Target ORF information:

RefSeq Version	XM_006512152.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512152.3

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGGCAG CTGCAGTGGC GGCGGCGGCG GCACCAGGGC CGAAGAGCAG 
ATCATGTCTG TCATCCTGCA GAGGCACGAG GCAGTGCCCG CCGGCCACAT ATACCGTATC 
AGCCCCAAGC AGTTGGTCAT CCCAGCTGGA GACCACAGGA CCATCTACAT CTCCTTCACG 
CCCACGGTGC TCGACCCAGG GGTCATGCAC AAGGTGGGGT ATACTGGCTA TGCCCTGGGC 
TTCATGAGCT TGGATAAGGA GACAGACAGA CAGATTCCAG GCCGAATGCG ACGCCTTCAG 
GACTTTGCCG TGAGCCCCCT GAGACTGGAT CTGAATGGCC ACGTGAGACA CGCGCAGCTG 
CACGTCGAGC TGGACAGCAG TGGCTACTTG GAATTCTGGT GCCAGGCCAG CGACCTTATC 
CCCCAAAATC CCTGCAGTGG GGTGCTAAGT GACCGGATTA CCACCCGCCA TCTGAAACTG 
ACCAACACCA CTGAAATCCT GTATGACTTC CAGGCCCGGG TCTCCAGGCC CTTCTTCATT 
TCTCAAGGTG GGGCCAGCTG GGACAGCAGA GCCCCTCATC AGTGTGAAGA AGGGACGGCC 
TCCACAGGCC AGTGGCTGAT GATCCGTCCT CAGGACAACA TGCTGGTGGA TGTGTCCTTC 
TCACTGTCCC TGGAACTGCT GTCCTATCAG AAACTCTCGG CCGACCAGAT GTTGCCCGGA 
GTGGATATTC AGGAGAGCGA GAACGGAGAG AGAAAGATGG TGTTCACACA GAACCTACTC 
CTGGGCTTCA CCAACCAGAC TGTTCAGGAG GTGCCCCTGC GGGCCTTTGT GACCCTGCCA 
GCGCCACAGC TCTCCACCAG CTGGGTGGAC TTCGGGACCT GTCTCGTGAA CCAGCAGCAC 
ACCCGGCAGA TCTACCTGAT GAACCTCAGC TGCTGCCTGA GCTACTGGAC GGTGCTGTTG 
GGACAAGAGG AACCAGCCAG GGAGGATCAT GCGTTTGGGG TCTCCCCAGG CAGTGGACTG 
CTGGAGGCCC GAGCCACCAA TGCACCCCCT ACCTCCAGCC CACTTCAGGT GTTCTTCAAC 
CCTAGGAGCA GCATCCTCTA TGAATCTACA CTGGTGGTGG AAGGCGTGCT GAGCGAGAAG 
CCCTGTGTCC TGAGGCTTCG GGGCCTAGGC TCTTACGATG AAAGATACGT GAAATTCCAT 
GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( XM_006512153.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512216.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X2, mRNA.

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CloneID	OMu43441
Clone ID Related Accession (Same CDS sequence)	XM_006512153.3 , XM_006512153.4
Accession Version	XM_006512153.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512153.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GACAAGCAGG CCAAAGAATA AGAAGTGGAT GGAACACCTG 
CAGGTGCCGC AGAGAGCCCT GGACAGGCGG CTGCTCGCCA GCATGGAGAA CAGGAATCAC 
TTCCTGAAAA ACCCTCGGTT TTTCCCGCCT AACACACCAC ATGGAGGCAA GTCTCTCATC 
GTCCCTTTGA GCAAGCCAGA GAGAAGAGCA TCCCTGAATG CAGAGCCTGA GTGGAGCTGT 
ACAGATGCCC CCGTGTTCCT GGCGAAGCCA TCAGTTGGAT TTTTCACAGA TTACGAAATT 
GGACCCGTTT ACGAGATGGT GATCGCTCTA CAGAACACCA CCTCCACCAG CCGATTCTTG 
CGTGTCCTCC CACCGTCCTC TCCGTACTTC GCTCTGGGAC TTGGGATGTT CCCAGGAAAA 
GGCGGAATAG TGGCTCCCGG GATGACCTGC CAGTACATCG TCCAGTTCAT CCCGGACTGT 
CTTGGCGATT TTGATGATTT CATTTTGGTG GAGACACAGT CTCCCCACAC GCTGGTAATC 
CCCCTGCAGG CCCGGAGACC GCCGCCAGTG CTGACCTTGT CACCAGTGTT GGACTGCGGC 
CACTGCCTCA TTGGAGGGGT GAAGATAACC AAATTCATCT GCAAAAATGT GGGCTTCAGC 
GTCGGCAAAT TCTGTATCAT GCCCCAAAAG AGCTGGCCGC CACCGAGTTT CCGGGCTGTT 
GCAACTACTG GCTTTGTAGA GCAGCCTCCT TTTGGAGTAA TGCCTTCCGT GTTTGAGCTG 
GCCCCAGGGT ACGCCATACT GTTGGAGGTC TTGTTCCTCC CTACTGGCCT AGGACAGGCA 
GAAGAGACCT TCATCATCGT GTGTGACAAC TGTCAGATAA AGGAGGTGGT CGTCGTAGGG 
ACAGGGCAGC TGGTGGCTTT GGATCTGATT CATATTTCTG GTGGAAAAAA TGAGCCAGAC 
CCAGGAGAGC TGAAAGATTT AACAGCCCAG TACTTTATCC GGTTTGAGCC TGAAAACGTC 
CAGTCCACCG CAAAGAAGCA GCTCATCATC AGAAATGCTA CACACGTGGA GCTGGCCTTC 
CACTGGCAGA TCATGAAACC CAACCTGCAG CCTCTGATGC CTGGAGAGAC ACACAGCTCA 
GACAGCATCA AATGCCACCC TGACAGGGAG ACAGCCTTCT CCATCATCCC CGAGAAGGGC 
ATCCTCGAGT CCCACTCGGA CCAAGAGTTC ATCCTGAGTT TTTCACCTTA CAAGCTCAAG 
TGTTTCCACA GTGTACTGCA AATGGTTCTA GAAGCTGTGC CCGAGCCCTT GAGCTCAGGG 
TTGGAGAACC TGGGGGACTA CTCCTACTCA GTGGATGACA TGATCGTCCT GGAAATCGAG 
GTGAAAGGCT TGGCGGAACC CTTCCAGGTC CTTTTAGAGC CGTACGCCCT CATCATCCCT 
GGGGAGAACT ACATTGGCAT CACCGTGAAG AAAGATTTTA AGATGTGGAA CAACAGCAAG 
TCACCCATCA GATACATGTG GGGGAAGATC AGCCACTGCC ATATCATCGA AGTGGAGCCC 
TGCACTGGGG TGATAGAGCC CAATGAGGTC GGAGATTTTG AGTTAAACCT AACGGGTGGG 
GTGCCTGGCC CAACCAGCCA GGACCTGGAG TGTGAAATCA AAGACTCACC ATGCCCGGTG 
GTACTCCACA TTGAGGCCTC ATTTAAGGGA CCTGCCTTGA CCATTGATGT CCCAGCCCTT 
CAGTTTGGTC TGCTTCGCCT GGGAAAGAAG GCTTCCATCT CCATCCAGAT CCGGAATGTC 
AGCCAGCTGT CCGCCGTGTG GCATTTGAAG GAGAGCCCAG TCTGCCTGGC AGAGAGAGAT 
GAGGATGTGT CCCCCTTCGA CATCGAGCCT TTCTGCGGCC AGCTTCTCCC TCTGGGGGAG 
TGCAGAGTGA CCATCACCCT TGAGGCCTCC CAGTGTCAGC GGCTGCAGAC AGTCCTGGAG 
CTGGAAGTGG AGAACGGGTC CGGGAGCTAC CTTCCCGTGT ACGCTGAGGT GCAGCAGCCT 
CACGTGTACC TGAAGAACAG CCACTTGGAG TTCAGCAACC TCTACCTGGG CGTGTCCACA 
AAGTCAAGGA CCACGCTGGT CAATGGCACA CTCCTGCCCA CCCGGTTCCA CTGGGGAAAG 
CTTCTGGGAC CTCAAGCAGA TTTGTGCACA ATGACAGTCT CTCCCACACA GGGCCTGCTG 
GGCCCCAGTG AAGAGCTCCA ACTCACCTTG GAGTTGACGA CCCACACAGA GGAAAAGCTG 
ACCAATTTGG TTCTCCTTTG TCACGTGTCT GGCATGGAGA AGCCGCTGGT CCTGGGCATC 
TCTGGGAAGC CCCTGGGACT TCAGGTGACC ATCTCCATAG TGGAATCTGA CGGAAGCAGC 
ACCACCAGCA CTACACAGGA GGAGCTGCGC TTGGACTTCG GCTCGAAGGT GCCACTGAGG 
ACGCCCGTGA TTCGCCATAT CATCCTGACA AACTGCTCCC CGATACAGAC CCCATTCACC 
CTCACACTGG AGTACTTCGG GAGCTCCCAG GACAGCCTGT ACAAGAAGAC CAGCGTCCCT 
GACGTGCCTC CTGCCCTGCT GAAGACAGCA CGCATCCAGC AGCACGTGGC CAAGAAGGAA 
AGGCTGGACT TTGTGGAGAG CATGTTATCT CACAGGAAAG GAGCTGCCTT CCTTCCCCAC 
ATCTCCCAGG GCATGCTGGG AGCCTACCAG GAGCTATGTA TTGACATCAC AGGCTGTGCC 
AACATGTGGG GCGAGTACTG GGATGAGCTC CTCTGCGTGG TGGGAGACCT GCCGGTCACT 
GTCATCCCGG TATACATGGC CGTGGTGGGC TGCCCTATCA GCTCCCTGAG GAATACGTGC 
TACAGTGTGG CCCCATTCCA GAAGGAGCCT ATCACCAGGT TTGGGACCCA GATCTCTGGA 
GGAGATACAG TCACCAGATC TCTCCGTCTG TATAACTCCA GTCACTGTGA CATCCGCCTA 
GACTGGGAGA CCTACATCCC AGAAGAAAAG GCGGACCGGC TGCTGGAGCT GCTTGTGTTT 
TATGGGCCAC CCTTCCCACT GCGAGATCAA GACGGCAGTG AGATTGTGTG CCCTGAGACC 
TCGGAGGCCA GCTTACCAGG GTCTCCATGC CCCTCCAATG TCTCAGTGTC TAGCCACACT 
GTCCCCTCAA CGGGCAGCTG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTGGATGT GTCCTTCTCA 
CTGTCCCTGG AACTGCTGTC CTATCAGAAA CTCTCGGCCG ACCAGATGTT GCCCGGAGTG 
GATATTCAGG AGAGCGAGAA CGGAGAGAGA AAGATGGTGT TCACACAGAA CCTACTCCTG 
GGCTTCACCA ACCAGACTGT TCAGGAGGTG CCCCTGCGGG CCTTTGTGAC CCTGCCAGCG 
CCACAGCTCT CCACCAGCTG GGTGGACTTC GGGACCTGTC TCGTGAACCA GCAGCACACC 
CGGCAGATCT ACCTGATGAA CCTCAGCTGC TGCCTGAGCT ACTGGACGGT GCTGTTGGGA 
CAAGAGGAAC CAGCCAGGGA GGATCATGCG TTTGGGGTCT CCCCAGGCAG TGGACTGCTG 
GAGGCCCGAG CCACCAATGC ACCCCCTACC TCCAGCCCAC TTCAGGTGTT CTTCAACCCT 
AGGAGCAGCA TCCTCTATGA ATCTACACTG GTGGTGGAAG GCGTGCTGAG CGAGAAGCCC 
TGTGTCCTGA GGCTTCGGGG CCTAGGCTCT TACGATGAAA GATACGTGAA ATTCCATGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512216.1
CDS	979..6204
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTGSCSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVDVSFSLSLELLSYQKLSADQMLPGVDIQESENGERKMVFTQNLLLGFTNQTVQEVPLRAFVTLPAPQLSTSWVDFGTCLVNQQHTRQIYLMNLSCCLSYWTVLLGQEEPAREDHAFGVSPGSGLLEARATNAPPTSSPLQVFFNPRSSILYESTLVVEGVLSEKPCVLRLRGLGSYDERYVKFHEF

Target ORF information:

RefSeq Version	XM_006512153.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512153.3

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GACAAGCAGG CCAAAGAATA AGAAGTGGAT GGAACACCTG 
CAGGTGCCGC AGAGAGCCCT GGACAGGCGG CTGCTCGCCA GCATGGAGAA CAGGAATCAC 
TTCCTGAAAA ACCCTCGGTT TTTCCCGCCT AACACACCAC ATGGAGGCAA GTCTCTCATC 
GTCCCTTTGA GCAAGCCAGA GAGAAGAGCA TCCCTGAATG CAGAGCCTGA GTGGAGCTGT 
ACAGATGCCC CCGTGTTCCT GGCGAAGCCA TCAGTTGGAT TTTTCACAGA TTACGAAATT 
GGACCCGTTT ACGAGATGGT GATCGCTCTA CAGAACACCA CCTCCACCAG CCGATTCTTG 
CGTGTCCTCC CACCGTCCTC TCCGTACTTC GCTCTGGGAC TTGGGATGTT CCCAGGAAAA 
GGCGGAATAG TGGCTCCCGG GATGACCTGC CAGTACATCG TCCAGTTCAT CCCGGACTGT 
CTTGGCGATT TTGATGATTT CATTTTGGTG GAGACACAGT CTCCCCACAC GCTGGTAATC 
CCCCTGCAGG CCCGGAGACC GCCGCCAGTG CTGACCTTGT CACCAGTGTT GGACTGCGGC 
CACTGCCTCA TTGGAGGGGT GAAGATAACC AAATTCATCT GCAAAAATGT GGGCTTCAGC 
GTCGGCAAAT TCTGTATCAT GCCCCAAAAG AGCTGGCCGC CACCGAGTTT CCGGGCTGTT 
GCAACTACTG GCTTTGTAGA GCAGCCTCCT TTTGGAGTAA TGCCTTCCGT GTTTGAGCTG 
GCCCCAGGGT ACGCCATACT GTTGGAGGTC TTGTTCCTCC CTACTGGCCT AGGACAGGCA 
GAAGAGACCT TCATCATCGT GTGTGACAAC TGTCAGATAA AGGAGGTGGT CGTCGTAGGG 
ACAGGGCAGC TGGTGGCTTT GGATCTGATT CATATTTCTG GTGGAAAAAA TGAGCCAGAC 
CCAGGAGAGC TGAAAGATTT AACAGCCCAG TACTTTATCC GGTTTGAGCC TGAAAACGTC 
CAGTCCACCG CAAAGAAGCA GCTCATCATC AGAAATGCTA CACACGTGGA GCTGGCCTTC 
CACTGGCAGA TCATGAAACC CAACCTGCAG CCTCTGATGC CTGGAGAGAC ACACAGCTCA 
GACAGCATCA AATGCCACCC TGACAGGGAG ACAGCCTTCT CCATCATCCC CGAGAAGGGC 
ATCCTCGAGT CCCACTCGGA CCAAGAGTTC ATCCTGAGTT TTTCACCTTA CAAGCTCAAG 
TGTTTCCACA GTGTACTGCA AATGGTTCTA GAAGCTGTGC CCGAGCCCTT GAGCTCAGGG 
TTGGAGAACC TGGGGGACTA CTCCTACTCA GTGGATGACA TGATCGTCCT GGAAATCGAG 
GTGAAAGGCT TGGCGGAACC CTTCCAGGTC CTTTTAGAGC CGTACGCCCT CATCATCCCT 
GGGGAGAACT ACATTGGCAT CACCGTGAAG AAAGATTTTA AGATGTGGAA CAACAGCAAG 
TCACCCATCA GATACATGTG GGGGAAGATC AGCCACTGCC ATATCATCGA AGTGGAGCCC 
TGCACTGGGG TGATAGAGCC CAATGAGGTC GGAGATTTTG AGTTAAACCT AACGGGTGGG 
GTGCCTGGCC CAACCAGCCA GGACCTGGAG TGTGAAATCA AAGACTCACC ATGCCCGGTG 
GTACTCCACA TTGAGGCCTC ATTTAAGGGA CCTGCCTTGA CCATTGATGT CCCAGCCCTT 
CAGTTTGGTC TGCTTCGCCT GGGAAAGAAG GCTTCCATCT CCATCCAGAT CCGGAATGTC 
AGCCAGCTGT CCGCCGTGTG GCATTTGAAG GAGAGCCCAG TCTGCCTGGC AGAGAGAGAT 
GAGGATGTGT CCCCCTTCGA CATCGAGCCT TTCTGCGGCC AGCTTCTCCC TCTGGGGGAG 
TGCAGAGTGA CCATCACCCT TGAGGCCTCC CAGTGTCAGC GGCTGCAGAC AGTCCTGGAG 
CTGGAAGTGG AGAACGGGTC CGGGAGCTAC CTTCCCGTGT ACGCTGAGGT GCAGCAGCCT 
CACGTGTACC TGAAGAACAG CCACTTGGAG TTCAGCAACC TCTACCTGGG CGTGTCCACA 
AAGTCAAGGA CCACGCTGGT CAATGGCACA CTCCTGCCCA CCCGGTTCCA CTGGGGAAAG 
CTTCTGGGAC CTCAAGCAGA TTTGTGCACA ATGACAGTCT CTCCCACACA GGGCCTGCTG 
GGCCCCAGTG AAGAGCTCCA ACTCACCTTG GAGTTGACGA CCCACACAGA GGAAAAGCTG 
ACCAATTTGG TTCTCCTTTG TCACGTGTCT GGCATGGAGA AGCCGCTGGT CCTGGGCATC 
TCTGGGAAGC CCCTGGGACT TCAGGTGACC ATCTCCATAG TGGAATCTGA CGGAAGCAGC 
ACCACCAGCA CTACACAGGA GGAGCTGCGC TTGGACTTCG GCTCGAAGGT GCCACTGAGG 
ACGCCCGTGA TTCGCCATAT CATCCTGACA AACTGCTCCC CGATACAGAC CCCATTCACC 
CTCACACTGG AGTACTTCGG GAGCTCCCAG GACAGCCTGT ACAAGAAGAC CAGCGTCCCT 
GACGTGCCTC CTGCCCTGCT GAAGACAGCA CGCATCCAGC AGCACGTGGC CAAGAAGGAA 
AGGCTGGACT TTGTGGAGAG CATGTTATCT CACAGGAAAG GAGCTGCCTT CCTTCCCCAC 
ATCTCCCAGG GCATGCTGGG AGCCTACCAG GAGCTATGTA TTGACATCAC AGGCTGTGCC 
AACATGTGGG GCGAGTACTG GGATGAGCTC CTCTGCGTGG TGGGAGACCT GCCGGTCACT 
GTCATCCCGG TATACATGGC CGTGGTGGGC TGCCCTATCA GCTCCCTGAG GAATACGTGC 
TACAGTGTGG CCCCATTCCA GAAGGAGCCT ATCACCAGGT TTGGGACCCA GATCTCTGGA 
GGAGATACAG TCACCAGATC TCTCCGTCTG TATAACTCCA GTCACTGTGA CATCCGCCTA 
GACTGGGAGA CCTACATCCC AGAAGAAAAG GCGGACCGGC TGCTGGAGCT GCTTGTGTTT 
TATGGGCCAC CCTTCCCACT GCGAGATCAA GACGGCAGTG AGATTGTGTG CCCTGAGACC 
TCGGAGGCCA GCTTACCAGG GTCTCCATGC CCCTCCAATG TCTCAGTGTC TAGCCACACT 
GTCCCCTCAA CGGGCAGCTG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTGGATGT GTCCTTCTCA 
CTGTCCCTGG AACTGCTGTC CTATCAGAAA CTCTCGGCCG ACCAGATGTT GCCCGGAGTG 
GATATTCAGG AGAGCGAGAA CGGAGAGAGA AAGATGGTGT TCACACAGAA CCTACTCCTG 
GGCTTCACCA ACCAGACTGT TCAGGAGGTG CCCCTGCGGG CCTTTGTGAC CCTGCCAGCG 
CCACAGCTCT CCACCAGCTG GGTGGACTTC GGGACCTGTC TCGTGAACCA GCAGCACACC 
CGGCAGATCT ACCTGATGAA CCTCAGCTGC TGCCTGAGCT ACTGGACGGT GCTGTTGGGA 
CAAGAGGAAC CAGCCAGGGA GGATCATGCG TTTGGGGTCT CCCCAGGCAG TGGACTGCTG 
GAGGCCCGAG CCACCAATGC ACCCCCTACC TCCAGCCCAC TTCAGGTGTT CTTCAACCCT 
AGGAGCAGCA TCCTCTATGA ATCTACACTG GTGGTGGAAG GCGTGCTGAG CGAGAAGCCC 
TGTGTCCTGA GGCTTCGGGG CCTAGGCTCT TACGATGAAA GATACGTGAA ATTCCATGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( XM_006512154.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512217.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X3, mRNA.

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CloneID	OMu43442
Clone ID Related Accession (Same CDS sequence)	XM_006512154.4 , XM_006512154.3
Accession Version	XM_006512154.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5226bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512154.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
ACCACCAGCA CTACACAGGA GGAGCTGCGC TTGGACTTCG GCTCGAAGGT GCCACTGAGG 
ACGCCCGTGA TTCGCCATAT CATCCTGACA AACTGCTCCC CGATACAGAC CCCATTCACC 
CTCACACTGG AGTACTTCGG GAGCTCCCAG GACAGCCTGT ACAAGAAGAC CAGCGTCCCT 
GACGTGCCTC CTGCCCTGCT GAAGACAGCA CGCATCCAGC AGCACGTGGC CAAGAAGGAA 
AGGCTGGACT TTGTGGAGAG CATGTTATCT CACAGGAAAG GAGCTGCCTT CCTTCCCCAC 
ATCTCCCAGG GCATGCTGGG AGCCTACCAG GAGCTATGTA TTGACATCAC AGGCTGTGCC 
AACATGTGGG GCGAGTACTG GGATGAGCTC CTCTGCGTGG TGGGAGACCT GCCGGTCACT 
GTCATCCCGG TATACATGGC CGTGGTGGGC TGCCCTATCA GCTCCCTGAG GAATACGTGC 
TACAGTGTGG CCCCATTCCA GAAGGAGCCT ATCACCAGGT TTGGGACCCA GATCTCTGGA 
GGAGATACAG TCACCAGATC TCTCCGTCTG TATAACTCCA GTCACTGTGA CATCCGCCTA 
GACTGGGAGA CCTACATCCC AGAAGAAAAG GCGGACCGGC TGCTGGAGCT GCTTGTGTTT 
TATGGGCCAC CCTTCCCACT GCGAGATCAA GACGGCAGTG AGATTGTGTG CCCTGAGACC 
TCGGAGGCCA GCTTACCAGG GTCTCCATGC CCCTCCAATG TCTCAGTGTC TAGCCACACT 
GTCCCCTCAA CGGGCAGCTG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTGGATGT GTCCTTCTCA 
CTGTCCCTGG AACTGCTGTC CTATCAGAAA CTCTCGGCCG ACCAGATGTT GCCCGGAGTG 
GATATTCAGG AGAGCGAGAA CGGAGAGAGA AAGATGGTGT TCACACAGAA CCTACTCCTG 
GGCTTCACCA ACCAGACTGT TCAGGAGGTG CCCCTGCGGG CCTTTGTGAC CCTGCCAGCG 
CCACAGCTCT CCACCAGCTG GGTGGACTTC GGGACCTGTC TCGTGAACCA GCAGCACACC 
CGGCAGATCT ACCTGATGAA CCTCAGCTGC TGCCTGAGCT ACTGGACGGT GCTGTTGGGA 
CAAGAGGAAC CAGCCAGGGA GGATCATGCG TTTGGGGTCT CCCCAGGCAG TGGACTGCTG 
GAGGCCCGAG CCACCAATGC ACCCCCTACC TCCAGCCCAC TTCAGGTGTT CTTCAACCCT 
AGGAGCAGCA TCCTCTATGA ATCTACACTG GTGGTGGAAG GCGTGCTGAG CGAGAAGCCC 
TGTGTCCTGA GGCTTCGGGG CCTAGGCTCT TACGATGAAA GATACGTGAA ATTCCATGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512217.1
CDS	979..6204
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTGSCSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVDVSFSLSLELLSYQKLSADQMLPGVDIQESENGERKMVFTQNLLLGFTNQTVQEVPLRAFVTLPAPQLSTSWVDFGTCLVNQQHTRQIYLMNLSCCLSYWTVLLGQEEPAREDHAFGVSPGSGLLEARATNAPPTSSPLQVFFNPRSSILYESTLVVEGVLSEKPCVLRLRGLGSYDERYVKFHEF

Target ORF information:

RefSeq Version	XM_006512154.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512154.3

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
ACCACCAGCA CTACACAGGA GGAGCTGCGC TTGGACTTCG GCTCGAAGGT GCCACTGAGG 
ACGCCCGTGA TTCGCCATAT CATCCTGACA AACTGCTCCC CGATACAGAC CCCATTCACC 
CTCACACTGG AGTACTTCGG GAGCTCCCAG GACAGCCTGT ACAAGAAGAC CAGCGTCCCT 
GACGTGCCTC CTGCCCTGCT GAAGACAGCA CGCATCCAGC AGCACGTGGC CAAGAAGGAA 
AGGCTGGACT TTGTGGAGAG CATGTTATCT CACAGGAAAG GAGCTGCCTT CCTTCCCCAC 
ATCTCCCAGG GCATGCTGGG AGCCTACCAG GAGCTATGTA TTGACATCAC AGGCTGTGCC 
AACATGTGGG GCGAGTACTG GGATGAGCTC CTCTGCGTGG TGGGAGACCT GCCGGTCACT 
GTCATCCCGG TATACATGGC CGTGGTGGGC TGCCCTATCA GCTCCCTGAG GAATACGTGC 
TACAGTGTGG CCCCATTCCA GAAGGAGCCT ATCACCAGGT TTGGGACCCA GATCTCTGGA 
GGAGATACAG TCACCAGATC TCTCCGTCTG TATAACTCCA GTCACTGTGA CATCCGCCTA 
GACTGGGAGA CCTACATCCC AGAAGAAAAG GCGGACCGGC TGCTGGAGCT GCTTGTGTTT 
TATGGGCCAC CCTTCCCACT GCGAGATCAA GACGGCAGTG AGATTGTGTG CCCTGAGACC 
TCGGAGGCCA GCTTACCAGG GTCTCCATGC CCCTCCAATG TCTCAGTGTC TAGCCACACT 
GTCCCCTCAA CGGGCAGCTG CAGTGGCGGC GGCGGCGGCA CCAGGGCCGA AGAGCAGATC 
ATGTCTGTCA TCCTGCAGAG GCACGAGGCA GTGCCCGCCG GCCACATATA CCGTATCAGC 
CCCAAGCAGT TGGTCATCCC AGCTGGAGAC CACAGGACCA TCTACATCTC CTTCACGCCC 
ACGGTGCTCG ACCCAGGGGT CATGCACAAG GTGGGGTATA CTGGCTATGC CCTGGGCTTC 
ATGAGCTTGG ATAAGGAGAC AGACAGACAG ATTCCAGGCC GAATGCGACG CCTTCAGGAC 
TTTGCCGTGA GCCCCCTGAG ACTGGATCTG AATGGCCACG TGAGACACGC GCAGCTGCAC 
GTCGAGCTGG ACAGCAGTGG CTACTTGGAA TTCTGGTGCC AGGCCAGCGA CCTTATCCCC 
CAAAATCCCT GCAGTGGGGT GCTAAGTGAC CGGATTACCA CCCGCCATCT GAAACTGACC 
AACACCACTG AAATCCTGTA TGACTTCCAG GCCCGGGTCT CCAGGCCCTT CTTCATTTCT 
CAAGGTGGGG CCAGCTGGGA CAGCAGAGCC CCTCATCAGT GTGAAGAAGG GACGGCCTCC 
ACAGGCCAGT GGCTGATGAT CCGTCCTCAG GACAACATGC TGGTGGATGT GTCCTTCTCA 
CTGTCCCTGG AACTGCTGTC CTATCAGAAA CTCTCGGCCG ACCAGATGTT GCCCGGAGTG 
GATATTCAGG AGAGCGAGAA CGGAGAGAGA AAGATGGTGT TCACACAGAA CCTACTCCTG 
GGCTTCACCA ACCAGACTGT TCAGGAGGTG CCCCTGCGGG CCTTTGTGAC CCTGCCAGCG 
CCACAGCTCT CCACCAGCTG GGTGGACTTC GGGACCTGTC TCGTGAACCA GCAGCACACC 
CGGCAGATCT ACCTGATGAA CCTCAGCTGC TGCCTGAGCT ACTGGACGGT GCTGTTGGGA 
CAAGAGGAAC CAGCCAGGGA GGATCATGCG TTTGGGGTCT CCCCAGGCAG TGGACTGCTG 
GAGGCCCGAG CCACCAATGC ACCCCCTACC TCCAGCCCAC TTCAGGTGTT CTTCAACCCT 
AGGAGCAGCA TCCTCTATGA ATCTACACTG GTGGTGGAAG GCGTGCTGAG CGAGAAGCCC 
TGTGTCCTGA GGCTTCGGGG CCTAGGCTCT TACGATGAAA GATACGTGAA ATTCCATGAG 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( XM_006512155.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512218.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X4, mRNA.

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CloneID	OMu43443
Clone ID Related Accession (Same CDS sequence)	XM_006512155.3 , XM_006512155.4
Accession Version	XM_006512155.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512155.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGGCAG CTGCAGTGGC GGCGGCGGCG GCACCAGGGC CGAAGAGCAG 
ATCATGTCTG TCATCCTGCA GAGGCACGAG GCAGTGCCCG CCGGCCACAT ATACCGTATC 
AGCCCCAAGC AGTTGGTCAT CCCAGCTGGA GACCACAGGA CCATCTACAT CTCCTTCACG 
CCCACGGTGC TCGACCCAGG GGTCATGCAC AAGGTGGGGT ATACTGGCTA TGCCCTGGGC 
TTCATGAGCT TGGATAAGGA GACAGACAGA CAGATTCCAG GCCGAATGCG ACGCCTTCAG 
GACTTTGCCG TGAGCCCCCT GAGACTGGAT CTGAATGGCC ACGTGAGACA CGCGCAGCTG 
CACGTCGAGC TGGACAGCAG TGGCTACTTG GAATTCTGGT GCCAGGCCAG CGACCTTATC 
CCCCAAAATC CCTGCAGTGG GGTGCTAAGT GACCGGATTA CCACCCGCCA TCTGAAACTG 
ACCAACACCA CTGAAATCCT GTATGACTTC CAGGCCCGGG TCTCCAGGCC CTTCTTCATT 
TCTCAAGGTG GGGCCAGCTG GGACAGCAGA GCCCCTCATC AGTGTGAAGA AGGGACGGCC 
TCCACAGGCC AGTGGCTGAT GATCCGTCCT CAGGACAACA TGCTGAAACT CTCGGCCGAC 
CAGATGTTGC CCGGAGTGGA TATTCAGGAG AGCGAGAACG GAGAGAGAAA GATGGTGTTC 
ACACAGAACC TACTCCTGGG CTTCACCAAC CAGACTGTTC AGGAGGTGCC CCTGCGGGCC 
TTTGTGACCC TGCCAGCGCC ACAGCTCTCC ACCAGCTGGG TGGACTTCGG GACCTGTCTC 
GTGAACCAGC AGCACACCCG GCAGATCTAC CTGATGAACC TCAGCTGCTG CCTGAGCTAC 
TGGACGGTGC TGTTGGGACA AGAGGAACCA GCCAGGGAGG ATCATGCGTT TGGGGTCTCC 
CCAGGCAGTG GACTGCTGGA GGCCCGAGCC ACCAATGCAC CCCCTACCTC CAGCCCACTT 
CAGGTGTTCT TCAACCCTAG GAGCAGCATC CTCTATGAAT CTACACTGGT GGTGGAAGGC 
GTGCTGAGCG AGAAGCCCTG TGTCCTGAGG CTTCGGGGCC TAGGCTCTTA CGATGAAAGA 
TACGTGAAAT TCCATGAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512218.1
CDS	979..6162
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTGSCSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLKLSADQMLPGVDIQESENGERKMVFTQNLLLGFTNQTVQEVPLRAFVTLPAPQLSTSWVDFGTCLVNQQHTRQIYLMNLSCCLSYWTVLLGQEEPAREDHAFGVSPGSGLLEARATNAPPTSSPLQVFFNPRSSILYESTLVVEGVLSEKPCVLRLRGLGSYDERYVKFHEF

Target ORF information:

RefSeq Version	XM_006512155.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512155.3

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GTCGGAGATT TTGAGTTAAA CCTAACGGGT 
GGGGTGCCTG GCCCAACCAG CCAGGACCTG GAGTGTGAAA TCAAAGACTC ACCATGCCCG 
GTGGTACTCC ACATTGAGGC CTCATTTAAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGGCAG CTGCAGTGGC GGCGGCGGCG GCACCAGGGC CGAAGAGCAG 
ATCATGTCTG TCATCCTGCA GAGGCACGAG GCAGTGCCCG CCGGCCACAT ATACCGTATC 
AGCCCCAAGC AGTTGGTCAT CCCAGCTGGA GACCACAGGA CCATCTACAT CTCCTTCACG 
CCCACGGTGC TCGACCCAGG GGTCATGCAC AAGGTGGGGT ATACTGGCTA TGCCCTGGGC 
TTCATGAGCT TGGATAAGGA GACAGACAGA CAGATTCCAG GCCGAATGCG ACGCCTTCAG 
GACTTTGCCG TGAGCCCCCT GAGACTGGAT CTGAATGGCC ACGTGAGACA CGCGCAGCTG 
CACGTCGAGC TGGACAGCAG TGGCTACTTG GAATTCTGGT GCCAGGCCAG CGACCTTATC 
CCCCAAAATC CCTGCAGTGG GGTGCTAAGT GACCGGATTA CCACCCGCCA TCTGAAACTG 
ACCAACACCA CTGAAATCCT GTATGACTTC CAGGCCCGGG TCTCCAGGCC CTTCTTCATT 
TCTCAAGGTG GGGCCAGCTG GGACAGCAGA GCCCCTCATC AGTGTGAAGA AGGGACGGCC 
TCCACAGGCC AGTGGCTGAT GATCCGTCCT CAGGACAACA TGCTGAAACT CTCGGCCGAC 
CAGATGTTGC CCGGAGTGGA TATTCAGGAG AGCGAGAACG GAGAGAGAAA GATGGTGTTC 
ACACAGAACC TACTCCTGGG CTTCACCAAC CAGACTGTTC AGGAGGTGCC CCTGCGGGCC 
TTTGTGACCC TGCCAGCGCC ACAGCTCTCC ACCAGCTGGG TGGACTTCGG GACCTGTCTC 
GTGAACCAGC AGCACACCCG GCAGATCTAC CTGATGAACC TCAGCTGCTG CCTGAGCTAC 
TGGACGGTGC TGTTGGGACA AGAGGAACCA GCCAGGGAGG ATCATGCGTT TGGGGTCTCC 
CCAGGCAGTG GACTGCTGGA GGCCCGAGCC ACCAATGCAC CCCCTACCTC CAGCCCACTT 
CAGGTGTTCT TCAACCCTAG GAGCAGCATC CTCTATGAAT CTACACTGGT GGTGGAAGGC 
GTGCTGAGCG AGAAGCCCTG TGTCCTGAGG CTTCGGGGCC TAGGCTCTTA CGATGAAAGA 
TACGTGAAAT TCCATGAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( XM_006512156.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512219.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OMu43444
Clone ID Related Accession (Same CDS sequence)	XM_006512156.3 , XM_006512156.4
Accession Version	XM_006512156.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512156.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGGCAG CTGCAGTGGC GGCGGCGGCG GCACCAGGGC CGAAGAGCAG 
ATCATGTCTG TCATCCTGCA GAGGCACGAG GCAGTGCCCG CCGGCCACAT ATACCGTATC 
AGCCCCAAGC AGTTGGTCAT CCCAGCTGGA GACCACAGGA CCATCTACAT CTCCTTCACG 
CCCACGGTGC TCGACCCAGG GGTCATGCAC AAGGTGGGGT ATACTGGCTA TGCCCTGGGC 
TTCATGAGCT TGGATAAGGA GACAGACAGA CAGATTCCAG GCCGAATGCG ACGCCTTCAG 
GACTTTGCCG TGAGCCCCCT GAGACTGGAT CTGAATGGCC ACGTGAGACA CGCGCAGCTG 
CACGTCGAGC TGGACAGCAG TGGCTACTTG GAATTCTGGT GCCAGGCCAG CGACCTTATC 
CCCCAAAATC CCTGCAGTGG GGTGCTAAGT GACCGGATTA CCACCCGCCA TCTGAAACTG 
ACCAACACCA CTGAAATCCT GTATGACTTC CAGGCCCGGG TCTCCAGGCC CTTCTTCATT 
TCTCAAGGTG GGGCCAGCTG GGACAGCAGA GCCCCTCATC AGTGTGAAGA AGGGACGGCC 
TCCACAGGCC AGTGGCTGAT GATCCGTCCT CAGGACAACA TGCTGGTGGA TGTGTCCTTC 
TCACTGTCCC TGGAACTGCT GTCCTATCAG AAACTCTCGG CCGACCAGAT GTTGCCCGGA 
GTGGATATTC AGGAGAGCGA GAACGGAGAG AGAAAGATGG TGTTCACACA GAACCTACTC 
CTGGGCTTCA CCAACCAGAC TGTTCAGGAG GTGCCCCTGC GGGCCTTTGT GACCCTGCCA 
GCGCCACAGC TCTCCACCAG CTGGGTGGAC TTCGGGACCT GTCTCGTGAA CCAGCAGCAC 
ACCCGGCAGA TCTACCTGAT GAACCTCAGC TGCTGCCTGA GCTACTGGAC GGTGCTGTTG 
GGACAAGAGG AACCAGCCAG GGAGGATCAT GCGTTTGGGG TCTCCCCAGG CAGTGGACTG 
CTGGAGGCCC GAGCCACCAA TGCACCCCCT ACCTCCAGCC CACTTCAGGT GTTCTTCAAC 
CCTAGGAGCA GCATCCTCTA TGAATCTACA CTGGTGGTGG AAGGCGTGCT GAGCGAGAAG 
CCCTGTGTCC TGAGGCTTCG GGGCCTAGGC TCTTACGATG AAAGATACGT GAAATTCCAT 
GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512219.1
CDS	979..6087
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTGSCSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVDVSFSLSLELLSYQKLSADQMLPGVDIQESENGERKMVFTQNLLLGFTNQTVQEVPLRAFVTLPAPQLSTSWVDFGTCLVNQQHTRQIYLMNLSCCLSYWTVLLGQEEPAREDHAFGVSPGSGLLEARATNAPPTSSPLQVFFNPRSSILYESTLVVEGVLSEKPCVLRLRGLGSYDERYVKFHEF

Target ORF information:

RefSeq Version	XM_006512156.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512156.3

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GAAGACAAGC AGGCCAAAGA ATAAGAAGTG GATGGAACAC 
CTGCAGGTGC CGCAGAGAGC CCTGGACAGG CGGCTGCTCG CCAGCATGGA GAACAGGAAT 
CACTTCCTGA AAAACCCTCG GTTTTTCCCG CCTAACACAC CACATGGAGG CAAGTCTCTC 
ATCGTCCCTT TGAGCAAGCC AGAGAGAAGA GCATCCCTGA ATGCAGAGCC TGAGTGGAGC 
TGTACAGATG CCCCCGTGTT CCTGGCGAAG CCATCAGTTG GATTTTTCAC AGATTACGAA 
ATTGGACCCG TTTACGAGAT GGTGATCGCT CTACAGAACA CCACCTCCAC CAGCCGATTC 
TTGCGTGTCC TCCCACCGTC CTCTCCGTAC TTCGCTCTGG GACTTGGGAT GTTCCCAGGA 
AAAGGCGGAA TAGTGGCTCC CGGGATGACC TGCCAGTACA TCGTCCAGTT CATCCCGGAC 
TGTCTTGGCG ATTTTGATGA TTTCATTTTG GTGGAGACAC AGTCTCCCCA CACGCTGGTA 
ATCCCCCTGC AGGCCCGGAG ACCGCCGCCA GTGCTGACCT TGTCACCAGT GTTGGACTGC 
GGCCACTGCC TCATTGGAGG GGTGAAGATA ACCAAATTCA TCTGCAAAAA TGTGGGCTTC 
AGCGTCGGCA AATTCTGTAT CATGCCCCAA AAGAGCTGGC CGCCACCGAG TTTCCGGGCT 
GTTGCAACTA CTGGCTTTGT AGAGCAGCCT CCTTTTGGAG TAATGCCTTC CGTGTTTGAG 
CTGGCCCCAG GGTACGCCAT ACTGTTGGAG GTCTTGTTCC TCCCTACTGG CCTAGGACAG 
GCAGAAGAGA CCTTCATCAT CGTGTGTGAC AACTGTCAGA TAAAGGAGGT GGTCGTCGTA 
GGGACAGGGC AGCTGGTGGC TTTGGATCTG ATTCATATTT CTGGTGGAAA AAATGAGCCA 
GACCCAGGAG AGCTGAAAGA TTTAACAGCC CAGTACTTTA TCCGGTTTGA GCCTGAAAAC 
GTCCAGTCCA CCGCAAAGAA GCAGCTCATC ATCAGAAATG CTACACACGT GGAGCTGGCC 
TTCCACTGGC AGATCATGAA ACCCAACCTG CAGCCTCTGA TGCCTGGAGA GACACACAGC 
TCAGACAGCA TCAAATGCCA CCCTGACAGG GAGACAGCCT TCTCCATCAT CCCCGAGAAG 
GGCATCCTCG AGTCCCACTC GGACCAAGAG TTCATCCTGA GTTTTTCACC TTACAAGCTC 
AAGTGTTTCC ACAGTGTACT GCAAATGGTT CTAGAAGCTG TGCCCGAGCC CTTGAGCTCA 
GGGTTGGAGA ACCTGGGGGA CTACTCCTAC TCAGTGGATG ACATGATCGT CCTGGAAATC 
GAGGTGAAAG GCTTGGCGGA ACCCTTCCAG GTCCTTTTAG AGCCGTACGC CCTCATCATC 
CCTGGGGAGA ACTACATTGG CATCACCGTG AAGAAAGATT TTAAGATGTG GAACAACAGC 
AAGTCACCCA TCAGATACAT GTGGGGGAAG ATCAGCCACT GCCATATCAT CGAAGTGGAG 
CCCTGCACTG GGGTGATAGA GCCCAATGAG GGACCTGCCT TGACCATTGA TGTCCCAGCC 
CTTCAGTTTG GTCTGCTTCG CCTGGGAAAG AAGGCTTCCA TCTCCATCCA GATCCGGAAT 
GTCAGCCAGC TGTCCGCCGT GTGGCATTTG AAGGAGAGCC CAGTCTGCCT GGCAGAGAGA 
GATGAGGATG TGTCCCCCTT CGACATCGAG CCTTTCTGCG GCCAGCTTCT CCCTCTGGGG 
GAGTGCAGAG TGACCATCAC CCTTGAGGCC TCCCAGTGTC AGCGGCTGCA GACAGTCCTG 
GAGCTGGAAG TGGAGAACGG GTCCGGGAGC TACCTTCCCG TGTACGCTGA GGTGCAGCAG 
CCTCACGTGT ACCTGAAGAA CAGCCACTTG GAGTTCAGCA ACCTCTACCT GGGCGTGTCC 
ACAAAGTCAA GGACCACGCT GGTCAATGGC ACACTCCTGC CCACCCGGTT CCACTGGGGA 
AAGCTTCTGG GACCTCAAGC AGATTTGTGC ACAATGACAG TCTCTCCCAC ACAGGGCCTG 
CTGGGCCCCA GTGAAGAGCT CCAACTCACC TTGGAGTTGA CGACCCACAC AGAGGAAAAG 
CTGACCAATT TGGTTCTCCT TTGTCACGTG TCTGGCATGG AGAAGCCGCT GGTCCTGGGC 
ATCTCTGGGA AGCCCCTGGG ACTTCAGGTG ACCATCTCCA TAGTGGAATC TGACGGAAGC 
AGCACCACCA GCACTACACA GGAGGAGCTG CGCTTGGACT TCGGCTCGAA GGTGCCACTG 
AGGACGCCCG TGATTCGCCA TATCATCCTG ACAAACTGCT CCCCGATACA GACCCCATTC 
ACCCTCACAC TGGAGTACTT CGGGAGCTCC CAGGACAGCC TGTACAAGAA GACCAGCGTC 
CCTGACGTGC CTCCTGCCCT GCTGAAGACA GCACGCATCC AGCAGCACGT GGCCAAGAAG 
GAAAGGCTGG ACTTTGTGGA GAGCATGTTA TCTCACAGGA AAGGAGCTGC CTTCCTTCCC 
CACATCTCCC AGGGCATGCT GGGAGCCTAC CAGGAGCTAT GTATTGACAT CACAGGCTGT 
GCCAACATGT GGGGCGAGTA CTGGGATGAG CTCCTCTGCG TGGTGGGAGA CCTGCCGGTC 
ACTGTCATCC CGGTATACAT GGCCGTGGTG GGCTGCCCTA TCAGCTCCCT GAGGAATACG 
TGCTACAGTG TGGCCCCATT CCAGAAGGAG CCTATCACCA GGTTTGGGAC CCAGATCTCT 
GGAGGAGATA CAGTCACCAG ATCTCTCCGT CTGTATAACT CCAGTCACTG TGACATCCGC 
CTAGACTGGG AGACCTACAT CCCAGAAGAA AAGGCGGACC GGCTGCTGGA GCTGCTTGTG 
TTTTATGGGC CACCCTTCCC ACTGCGAGAT CAAGACGGCA GTGAGATTGT GTGCCCTGAG 
ACCTCGGAGG CCAGCTTACC AGGGTCTCCA TGCCCCTCCA ATGTCTCAGT GTCTAGCCAC 
ACTGTCCCCT CAACGGGCAG CTGCAGTGGC GGCGGCGGCG GCACCAGGGC CGAAGAGCAG 
ATCATGTCTG TCATCCTGCA GAGGCACGAG GCAGTGCCCG CCGGCCACAT ATACCGTATC 
AGCCCCAAGC AGTTGGTCAT CCCAGCTGGA GACCACAGGA CCATCTACAT CTCCTTCACG 
CCCACGGTGC TCGACCCAGG GGTCATGCAC AAGGTGGGGT ATACTGGCTA TGCCCTGGGC 
TTCATGAGCT TGGATAAGGA GACAGACAGA CAGATTCCAG GCCGAATGCG ACGCCTTCAG 
GACTTTGCCG TGAGCCCCCT GAGACTGGAT CTGAATGGCC ACGTGAGACA CGCGCAGCTG 
CACGTCGAGC TGGACAGCAG TGGCTACTTG GAATTCTGGT GCCAGGCCAG CGACCTTATC 
CCCCAAAATC CCTGCAGTGG GGTGCTAAGT GACCGGATTA CCACCCGCCA TCTGAAACTG 
ACCAACACCA CTGAAATCCT GTATGACTTC CAGGCCCGGG TCTCCAGGCC CTTCTTCATT 
TCTCAAGGTG GGGCCAGCTG GGACAGCAGA GCCCCTCATC AGTGTGAAGA AGGGACGGCC 
TCCACAGGCC AGTGGCTGAT GATCCGTCCT CAGGACAACA TGCTGGTGGA TGTGTCCTTC 
TCACTGTCCC TGGAACTGCT GTCCTATCAG AAACTCTCGG CCGACCAGAT GTTGCCCGGA 
GTGGATATTC AGGAGAGCGA GAACGGAGAG AGAAAGATGG TGTTCACACA GAACCTACTC 
CTGGGCTTCA CCAACCAGAC TGTTCAGGAG GTGCCCCTGC GGGCCTTTGT GACCCTGCCA 
GCGCCACAGC TCTCCACCAG CTGGGTGGAC TTCGGGACCT GTCTCGTGAA CCAGCAGCAC 
ACCCGGCAGA TCTACCTGAT GAACCTCAGC TGCTGCCTGA GCTACTGGAC GGTGCTGTTG 
GGACAAGAGG AACCAGCCAG GGAGGATCAT GCGTTTGGGG TCTCCCCAGG CAGTGGACTG 
CTGGAGGCCC GAGCCACCAA TGCACCCCCT ACCTCCAGCC CACTTCAGGT GTTCTTCAAC 
CCTAGGAGCA GCATCCTCTA TGAATCTACA CTGGTGGTGG AAGGCGTGCT GAGCGAGAAG 
CCCTGTGTCC TGAGGCTTCG GGGCCTAGGC TCTTACGATG AAAGATACGT GAAATTCCAT 
GAGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dlec1 ( XM_006512157.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006512220.1 Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X6, mRNA.

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CloneID	OMu43445
Clone ID Related Accession (Same CDS sequence)	NM_001368821.1 , XM_006512157.3
Accession Version	XM_006512157.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-21
Organism	Mus musculus(house mouse)
Product	deleted in lung and esophageal cancer protein 1 homolog isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000075.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006512157.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Mus musculus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GACAAGCAGG CCAAAGAATA AGAAGTGGAT GGAACACCTG 
CAGGTGCCGC AGAGAGCCCT GGACAGGCGG CTGCTCGCCA GCATGGAGAA CAGGAATCAC 
TTCCTGAAAA ACCCTCGGTT TTTCCCGCCT AACACACCAC ATGGAGGCAA GTCTCTCATC 
GTCCCTTTGA GCAAGCCAGA GAGAAGAGCA TCCCTGAATG CAGAGCCTGA GTGGAGCTGT 
ACAGATGCCC CCGTGTTCCT GGCGAAGCCA TCAGTTGGAT TTTTCACAGA TTACGAAATT 
GGACCCGTTT ACGAGATGGT GATCGCTCTA CAGAACACCA CCTCCACCAG CCGATTCTTG 
CGTGTCCTCC CACCGTCCTC TCCGTACTTC GCTCTGGGAC TTGGGATGTT CCCAGGAAAA 
GGCGGAATAG TGGCTCCCGG GATGACCTGC CAGTACATCG TCCAGTTCAT CCCGGACTGT 
CTTGGCGATT TTGATGATTT CATTTTGGTG GAGACACAGT CTCCCCACAC GCTGGTAATC 
CCCCTGCAGG CCCGGAGACC GCCGCCAGTG CTGACCTTGT CACCAGTGTT GGACTGCGGC 
CACTGCCTCA TTGGAGGGGT GAAGATAACC AAATTCATCT GCAAAAATGT GGGCTTCAGC 
GTCGGCAAAT TCTGTATCAT GCCCCAAAAG AGCTGGCCGC CACCGAGTTT CCGGGCTGTT 
GCAACTACTG GCTTTGTAGA GCAGCCTCCT TTTGGAGTAA TGCCTTCCGT GTTTGAGCTG 
GCCCCAGGGT ACGCCATACT GTTGGAGGTC TTGTTCCTCC CTACTGGCCT AGGACAGGCA 
GAAGAGACCT TCATCATCGT GTGTGACAAC TGTCAGATAA AGGAGGTGGT CGTCGTAGGG 
ACAGGGCAGC TGGTGGCTTT GGATCTGATT CATATTTCTG GTGGAAAAAA TGAGCCAGAC 
CCAGGAGAGC TGAAAGATTT AACAGCCCAG TACTTTATCC GGTTTGAGCC TGAAAACGTC 
CAGTCCACCG CAAAGAAGCA GCTCATCATC AGAAATGCTA CACACGTGGA GCTGGCCTTC 
CACTGGCAGA TCATGAAACC CAACCTGCAG CCTCTGATGC CTGGAGAGAC ACACAGCTCA 
GACAGCATCA AATGCCACCC TGACAGGGAG ACAGCCTTCT CCATCATCCC CGAGAAGGGC 
ATCCTCGAGT CCCACTCGGA CCAAGAGTTC ATCCTGAGTT TTTCACCTTA CAAGCTCAAG 
TGTTTCCACA GTGTACTGCA AATGGTTCTA GAAGCTGTGC CCGAGCCCTT GAGCTCAGGG 
TTGGAGAACC TGGGGGACTA CTCCTACTCA GTGGATGACA TGATCGTCCT GGAAATCGAG 
GTGAAAGGCT TGGCGGAACC CTTCCAGGTC CTTTTAGAGC CGTACGCCCT CATCATCCCT 
GGGGAGAACT ACATTGGCAT CACCGTGAAG AAAGATTTTA AGATGTGGAA CAACAGCAAG 
TCACCCATCA GATACATGTG GGGGAAGATC AGCCACTGCC ATATCATCGA AGTGGAGCCC 
TGCACTGGGG TGATAGAGCC CAATGAGGTC GGAGATTTTG AGTTAAACCT AACGGGTGGG 
GTGCCTGGCC CAACCAGCCA GGACCTGGAG TGTGAAATCA AAGACTCACC ATGCCCGGTG 
GTACTCCACA TTGAGGCCTC ATTTAAGGGA CCTGCCTTGA CCATTGATGT CCCAGCCCTT 
CAGTTTGGTC TGCTTCGCCT GGGAAAGAAG GCTTCCATCT CCATCCAGAT CCGGAATGTC 
AGCCAGCTGT CCGCCGTGTG GCATTTGAAG GAGAGCCCAG TCTGCCTGGC AGAGAGAGAT 
GAGGATGTGT CCCCCTTCGA CATCGAGCCT TTCTGCGGCC AGCTTCTCCC TCTGGGGGAG 
TGCAGAGTGA CCATCACCCT TGAGGCCTCC CAGTGTCAGC GGCTGCAGAC AGTCCTGGAG 
CTGGAAGTGG AGAACGGGTC CGGGAGCTAC CTTCCCGTGT ACGCTGAGGT GCAGCAGCCT 
CACGTGTACC TGAAGAACAG CCACTTGGAG TTCAGCAACC TCTACCTGGG CGTGTCCACA 
AAGTCAAGGA CCACGCTGGT CAATGGCACA CTCCTGCCCA CCCGGTTCCA CTGGGGAAAG 
CTTCTGGGAC CTCAAGCAGA TTTGTGCACA ATGACAGTCT CTCCCACACA GGGCCTGCTG 
GGCCCCAGTG AAGAGCTCCA ACTCACCTTG GAGTTGACGA CCCACACAGA GGAAAAGCTG 
ACCAATTTGG TTCTCCTTTG TCACGTGTCT GGCATGGAGA AGCCGCTGGT CCTGGGCATC 
TCTGGGAAGC CCCTGGGACT TCAGGTGACC ATCTCCATAG TGGAATCTGA CGGAAGCACC 
ACCAGCACTA CACAGGAGGA GCTGCGCTTG GACTTCGGCT CGAAGGTGCC ACTGAGGACG 
CCCGTGATTC GCCATATCAT CCTGACAAAC TGCTCCCCGA TACAGACCCC ATTCACCCTC 
ACACTGGAGT ACTTCGGGAG CTCCCAGGAC AGCCTGTACA AGAAGACCAG CGTCCCTGAC 
GTGCCTCCTG CCCTGCTGAA GACAGCACGC ATCCAGCAGC ACGTGGCCAA GAAGGAAAGG 
CTGGACTTTG TGGAGAGCAT GTTATCTCAC AGGAAAGGAG CTGCCTTCCT TCCCCACATC 
TCCCAGGGCA TGCTGGGAGC CTACCAGGAG CTATGTATTG ACATCACAGG CTGTGCCAAC 
ATGTGGGGCG AGTACTGGGA TGAGCTCCTC TGCGTGGTGG GAGACCTGCC GGTCACTGTC 
ATCCCGGTAT ACATGGCCGT GGTGGGCTGC CCTATCAGCT CCCTGAGGAA TACGTGCTAC 
AGTGTGGCCC CATTCCAGAA GGAGCCTATC ACCAGGTTTG GGACCCAGAT CTCTGGAGGA 
GATACAGTCA CCAGATCTCT CCGTCTGTAT AACTCCAGTC ACTGTGACAT CCGCCTAGAC 
TGGGAGACCT ACATCCCAGA AGAAAAGGCG GACCGGCTGC TGGAGCTGCT TGTGTTTTAT 
GGGCCACCCT TCCCACTGCG AGATCAAGAC GGCAGTGAGA TTGTGTGCCC TGAGACCTCG 
GAGGCCAGCT TACCAGGGTC TCCATGCCCC TCCAATGTCT CAGTGTCTAG CCACACTGTC 
CCCTCAACGG GCAGCTGCAG TGGCGGCGGC GGCGGCACCA GGGCCGAAGA GCAGATCATG 
TCTGTCATCC TGCAGAGGCA CGAGGCAGTG CCCGCCGGCC ACATATACCG TATCAGCCCC 
AAGCAGTTGG TCATCCCAGC TGGAGACCAC AGGACCATCT ACATCTCCTT CACGCCCACG 
GTGCTCGACC CAGGGGTCAT GCACAAGGTG GGGTATACTG GCTATGCCCT GGGCTTCATG 
AGCTTGGATA AGGAGACAGA CAGACAGATT CCAGGCCGAA TGCGACGCCT TCAGGACTTT 
GCCGTGAGCC CCCTGAGACT GGATCTGAAT GGCCACGTGA GACACGCGCA GCTGCACGTC 
GAGCTGGACA GCAGTGGCTA CTTGGAATTC TGGTGCCAGG CCAGCGACCT TATCCCCCAA 
AATCCCTGCA GTGGGGTGCT AAGTGACCGG ATTACCACCC GCCATCTGAA ACTGACCAAC 
ACCACTGAAA TCCTGTATGA CTTCCAGGCC CGGGTCTCCA GGCCCTTCTT CATTTCTCAA 
GGTGGGGCCA GCTGGGACAG CAGAGCCCCT CATCAGTGTG AAGAAGGGAC GGCCTCCACA 
GGCCAGTGGC TGATGATCCG TCCTCAGGAC AACATGCTGG TCAGTGCAGG GGTGGAGCGT 
CTCCCCCACC ACCCCCGCCT CTATAGGCAA CACACAGGCA CATCCTGCCC ACATCTGGGA 
ACGAGGTACA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006512220.1
CDS	980..5734
Translation	MDSESKEEQQCTASSAVMSSLETQFLSVPHQLESASLSWSIRKSLHSSTVLMDDCHFGFPTRSLRAGFHALPPLPEPQTLRLRPASLRTQEISHLLARVFRNLYTAQVIGDDLSDSLIKARGSEDARHEEFLDQLQQARAIYKQRLDEAAMLERHIMQARARDIAETEHTTKQSRLQVVETPVKLPPVKTAFRWCLDSKLMRKHHLISTEDYYTDPVPFCPAPKDKSTLGCSKLTFSCEQRFLRKSELDREAEEHCRQLTECDDFEYTVDSLGSISKAKERTTDSVKTSRPKNKKWMEHLQVPQRALDRRLLASMENRNHFLKNPRFFPPNTPHGGKSLIVPLSKPERRASLNAEPEWSCTDAPVFLAKPSVGFFTDYEIGPVYEMVIALQNTTSTSRFLRVLPPSSPYFALGLGMFPGKGGIVAPGMTCQYIVQFIPDCLGDFDDFILVETQSPHTLVIPLQARRPPPVLTLSPVLDCGHCLIGGVKITKFICKNVGFSVGKFCIMPQKSWPPPSFRAVATTGFVEQPPFGVMPSVFELAPGYAILLEVLFLPTGLGQAEETFIIVCDNCQIKEVVVVGTGQLVALDLIHISGGKNEPDPGELKDLTAQYFIRFEPENVQSTAKKQLIIRNATHVELAFHWQIMKPNLQPLMPGETHSSDSIKCHPDRETAFSIIPEKGILESHSDQEFILSFSPYKLKCFHSVLQMVLEAVPEPLSSGLENLGDYSYSVDDMIVLEIEVKGLAEPFQVLLEPYALIIPGENYIGITVKKDFKMWNNSKSPIRYMWGKISHCHIIEVEPCTGVIEPNEVGDFELNLTGGVPGPTSQDLECEIKDSPCPVVLHIEASFKGPALTIDVPALQFGLLRLGKKASISIQIRNVSQLSAVWHLKESPVCLAERDEDVSPFDIEPFCGQLLPLGECRVTITLEASQCQRLQTVLELEVENGSGSYLPVYAEVQQPHVYLKNSHLEFSNLYLGVSTKSRTTLVNGTLLPTRFHWGKLLGPQADLCTMTVSPTQGLLGPSEELQLTLELTTHTEEKLTNLVLLCHVSGMEKPLVLGISGKPLGLQVTISIVESDGSTTSTTQEELRLDFGSKVPLRTPVIRHIILTNCSPIQTPFTLTLEYFGSSQDSLYKKTSVPDVPPALLKTARIQQHVAKKERLDFVESMLSHRKGAAFLPHISQGMLGAYQELCIDITGCANMWGEYWDELLCVVGDLPVTVIPVYMAVVGCPISSLRNTCYSVAPFQKEPITRFGTQISGGDTVTRSLRLYNSSHCDIRLDWETYIPEEKADRLLELLVFYGPPFPLRDQDGSEIVCPETSEASLPGSPCPSNVSVSSHTVPSTGSCSGGGGGTRAEEQIMSVILQRHEAVPAGHIYRISPKQLVIPAGDHRTIYISFTPTVLDPGVMHKVGYTGYALGFMSLDKETDRQIPGRMRRLQDFAVSPLRLDLNGHVRHAQLHVELDSSGYLEFWCQASDLIPQNPCSGVLSDRITTRHLKLTNTTEILYDFQARVSRPFFISQGGASWDSRAPHQCEEGTASTGQWLMIRPQDNMLVSAGVERLPHHPRLYRQHTGTSCPHLGTRYK

Target ORF information:

RefSeq Version	XM_006512157.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus deleted in lung and esophageal cancer 1 (Dlec1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006512157.3

ORF Insert Sequence:

ATGGATTCCG AGAGCAAGGA AGAGCAGCAA TGCACAGCTT CCTCGGCCGT TATGTCGTCC 
TTGGAGACCC AGTTTTTGTC TGTCCCACAC CAGCTCGAGT CCGCCAGTCT CAGCTGGTCC 
ATCAGGAAGT CCTTGCATTC CTCGACCGTC TTAATGGATG ATTGCCATTT CGGCTTCCCT 
ACGCGGTCCC TCCGCGCCGG GTTCCACGCG CTCCCTCCAC TCCCGGAGCC GCAGACGCTG 
CGTCTACGCC CCGCCTCGTT GCGCACGCAG GAAATCTCAC ATCTGCTTGC CCGAGTCTTC 
CGAAACTTGT ACACCGCCCA GGTGATCGGA GATGACTTGA GCGACAGTTT GATCAAGGCC 
CGCGGCAGTG AGGATGCGCG CCACGAAGAG TTCCTTGACC AGCTGCAGCA GGCGCGAGCC 
ATCTATAAAC AGCGTCTGGA TGAAGCCGCC ATGTTGGAGA GACATATCAT GCAGGCCCGT 
GCACGGGATA TTGCAGAAAC AGAACACACC ACCAAACAGA GCAGACTACA GGTTGTGGAG 
ACCCCTGTCA AACTGCCCCC AGTGAAGACA GCCTTCAGGT GGTGTCTAGA CAGCAAGTTA 
ATGCGAAAAC ATCACTTAAT CTCTACAGAG GATTACTACA CTGATCCCGT GCCGTTTTGC 
CCAGCACCCA AAGACAAATC CACCCTTGGA TGTTCAAAGC TGACGTTTAG CTGTGAGCAG 
CGTTTCCTGC GCAAGTCAGA GCTGGACAGG GAGGCTGAGG AGCACTGCAG GCAGCTCACA 
GAGTGTGACG ACTTTGAGTA CACCGTGGAC AGCTTGGGCT CCATCTCGAA GGCCAAAGAG 
AGGACCACAG ATTCTGTCAA GACAAGCAGG CCAAAGAATA AGAAGTGGAT GGAACACCTG 
CAGGTGCCGC AGAGAGCCCT GGACAGGCGG CTGCTCGCCA GCATGGAGAA CAGGAATCAC 
TTCCTGAAAA ACCCTCGGTT TTTCCCGCCT AACACACCAC ATGGAGGCAA GTCTCTCATC 
GTCCCTTTGA GCAAGCCAGA GAGAAGAGCA TCCCTGAATG CAGAGCCTGA GTGGAGCTGT 
ACAGATGCCC CCGTGTTCCT GGCGAAGCCA TCAGTTGGAT TTTTCACAGA TTACGAAATT 
GGACCCGTTT ACGAGATGGT GATCGCTCTA CAGAACACCA CCTCCACCAG CCGATTCTTG 
CGTGTCCTCC CACCGTCCTC TCCGTACTTC GCTCTGGGAC TTGGGATGTT CCCAGGAAAA 
GGCGGAATAG TGGCTCCCGG GATGACCTGC CAGTACATCG TCCAGTTCAT CCCGGACTGT 
CTTGGCGATT TTGATGATTT CATTTTGGTG GAGACACAGT CTCCCCACAC GCTGGTAATC 
CCCCTGCAGG CCCGGAGACC GCCGCCAGTG CTGACCTTGT CACCAGTGTT GGACTGCGGC 
CACTGCCTCA TTGGAGGGGT GAAGATAACC AAATTCATCT GCAAAAATGT GGGCTTCAGC 
GTCGGCAAAT TCTGTATCAT GCCCCAAAAG AGCTGGCCGC CACCGAGTTT CCGGGCTGTT 
GCAACTACTG GCTTTGTAGA GCAGCCTCCT TTTGGAGTAA TGCCTTCCGT GTTTGAGCTG 
GCCCCAGGGT ACGCCATACT GTTGGAGGTC TTGTTCCTCC CTACTGGCCT AGGACAGGCA 
GAAGAGACCT TCATCATCGT GTGTGACAAC TGTCAGATAA AGGAGGTGGT CGTCGTAGGG 
ACAGGGCAGC TGGTGGCTTT GGATCTGATT CATATTTCTG GTGGAAAAAA TGAGCCAGAC 
CCAGGAGAGC TGAAAGATTT AACAGCCCAG TACTTTATCC GGTTTGAGCC TGAAAACGTC 
CAGTCCACCG CAAAGAAGCA GCTCATCATC AGAAATGCTA CACACGTGGA GCTGGCCTTC 
CACTGGCAGA TCATGAAACC CAACCTGCAG CCTCTGATGC CTGGAGAGAC ACACAGCTCA 
GACAGCATCA AATGCCACCC TGACAGGGAG ACAGCCTTCT CCATCATCCC CGAGAAGGGC 
ATCCTCGAGT CCCACTCGGA CCAAGAGTTC ATCCTGAGTT TTTCACCTTA CAAGCTCAAG 
TGTTTCCACA GTGTACTGCA AATGGTTCTA GAAGCTGTGC CCGAGCCCTT GAGCTCAGGG 
TTGGAGAACC TGGGGGACTA CTCCTACTCA GTGGATGACA TGATCGTCCT GGAAATCGAG 
GTGAAAGGCT TGGCGGAACC CTTCCAGGTC CTTTTAGAGC CGTACGCCCT CATCATCCCT 
GGGGAGAACT ACATTGGCAT CACCGTGAAG AAAGATTTTA AGATGTGGAA CAACAGCAAG 
TCACCCATCA GATACATGTG GGGGAAGATC AGCCACTGCC ATATCATCGA AGTGGAGCCC 
TGCACTGGGG TGATAGAGCC CAATGAGGTC GGAGATTTTG AGTTAAACCT AACGGGTGGG 
GTGCCTGGCC CAACCAGCCA GGACCTGGAG TGTGAAATCA AAGACTCACC ATGCCCGGTG 
GTACTCCACA TTGAGGCCTC ATTTAAGGGA CCTGCCTTGA CCATTGATGT CCCAGCCCTT 
CAGTTTGGTC TGCTTCGCCT GGGAAAGAAG GCTTCCATCT CCATCCAGAT CCGGAATGTC 
AGCCAGCTGT CCGCCGTGTG GCATTTGAAG GAGAGCCCAG TCTGCCTGGC AGAGAGAGAT 
GAGGATGTGT CCCCCTTCGA CATCGAGCCT TTCTGCGGCC AGCTTCTCCC TCTGGGGGAG 
TGCAGAGTGA CCATCACCCT TGAGGCCTCC CAGTGTCAGC GGCTGCAGAC AGTCCTGGAG 
CTGGAAGTGG AGAACGGGTC CGGGAGCTAC CTTCCCGTGT ACGCTGAGGT GCAGCAGCCT 
CACGTGTACC TGAAGAACAG CCACTTGGAG TTCAGCAACC TCTACCTGGG CGTGTCCACA 
AAGTCAAGGA CCACGCTGGT CAATGGCACA CTCCTGCCCA CCCGGTTCCA CTGGGGAAAG 
CTTCTGGGAC CTCAAGCAGA TTTGTGCACA ATGACAGTCT CTCCCACACA GGGCCTGCTG 
GGCCCCAGTG AAGAGCTCCA ACTCACCTTG GAGTTGACGA CCCACACAGA GGAAAAGCTG 
ACCAATTTGG TTCTCCTTTG TCACGTGTCT GGCATGGAGA AGCCGCTGGT CCTGGGCATC 
TCTGGGAAGC CCCTGGGACT TCAGGTGACC ATCTCCATAG TGGAATCTGA CGGAAGCACC 
ACCAGCACTA CACAGGAGGA GCTGCGCTTG GACTTCGGCT CGAAGGTGCC ACTGAGGACG 
CCCGTGATTC GCCATATCAT CCTGACAAAC TGCTCCCCGA TACAGACCCC ATTCACCCTC 
ACACTGGAGT ACTTCGGGAG CTCCCAGGAC AGCCTGTACA AGAAGACCAG CGTCCCTGAC 
GTGCCTCCTG CCCTGCTGAA GACAGCACGC ATCCAGCAGC ACGTGGCCAA GAAGGAAAGG 
CTGGACTTTG TGGAGAGCAT GTTATCTCAC AGGAAAGGAG CTGCCTTCCT TCCCCACATC 
TCCCAGGGCA TGCTGGGAGC CTACCAGGAG CTATGTATTG ACATCACAGG CTGTGCCAAC 
ATGTGGGGCG AGTACTGGGA TGAGCTCCTC TGCGTGGTGG GAGACCTGCC GGTCACTGTC 
ATCCCGGTAT ACATGGCCGT GGTGGGCTGC CCTATCAGCT CCCTGAGGAA TACGTGCTAC 
AGTGTGGCCC CATTCCAGAA GGAGCCTATC ACCAGGTTTG GGACCCAGAT CTCTGGAGGA 
GATACAGTCA CCAGATCTCT CCGTCTGTAT AACTCCAGTC ACTGTGACAT CCGCCTAGAC 
TGGGAGACCT ACATCCCAGA AGAAAAGGCG GACCGGCTGC TGGAGCTGCT TGTGTTTTAT 
GGGCCACCCT TCCCACTGCG AGATCAAGAC GGCAGTGAGA TTGTGTGCCC TGAGACCTCG 
GAGGCCAGCT TACCAGGGTC TCCATGCCCC TCCAATGTCT CAGTGTCTAG CCACACTGTC 
CCCTCAACGG GCAGCTGCAG TGGCGGCGGC GGCGGCACCA GGGCCGAAGA GCAGATCATG 
TCTGTCATCC TGCAGAGGCA CGAGGCAGTG CCCGCCGGCC ACATATACCG TATCAGCCCC 
AAGCAGTTGG TCATCCCAGC TGGAGACCAC AGGACCATCT ACATCTCCTT CACGCCCACG 
GTGCTCGACC CAGGGGTCAT GCACAAGGTG GGGTATACTG GCTATGCCCT GGGCTTCATG 
AGCTTGGATA AGGAGACAGA CAGACAGATT CCAGGCCGAA TGCGACGCCT TCAGGACTTT 
GCCGTGAGCC CCCTGAGACT GGATCTGAAT GGCCACGTGA GACACGCGCA GCTGCACGTC 
GAGCTGGACA GCAGTGGCTA CTTGGAATTC TGGTGCCAGG CCAGCGACCT TATCCCCCAA 
AATCCCTGCA GTGGGGTGCT AAGTGACCGG ATTACCACCC GCCATCTGAA ACTGACCAAC 
ACCACTGAAA TCCTGTATGA CTTCCAGGCC CGGGTCTCCA GGCCCTTCTT CATTTCTCAA 
GGTGGGGCCA GCTGGGACAG CAGAGCCCCT CATCAGTGTG AAGAAGGGAC GGCCTCCACA 
GGCCAGTGGC TGATGATCCG TCCTCAGGAC AACATGCTGG TCAGTGCAGG GGTGGAGCGT 
CTCCCCCACC ACCCCCGCCT CTATAGGCAA CACACAGGCA CATCCTGCCC ACATCTGGGA 
ACGAGGTACA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Dlec1
Entrez Gene ID	320256
Full Name	deleted in lung and esophageal cancer 1
Synonyms	D630005C06Rik
General protein information	Preferred Names deleted in lung and esophageal cancer 1 Names deleted in lung and esophageal cancer protein 1 homolog dlec1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 9 Map Location: 9\|9 F3

Mus musculus (house mouse)	Dlec1	NP_796091.2
Rattus norvegicus (Norway rat)	Dlec1	XP_006244203.1
Danio rerio (zebrafish)	dlec1	XP_683226.4
Homo sapiens (human)	DLEC1	NP_031361.2
Pan troglodytes (chimpanzee)	DLEC1	XP_003309770.1
Gallus gallus (chicken)	DLEC1	XP_003640722.2
Xenopus tropicalis (tropical clawed frog)	dlec1	XP_002938052.2
	DLEC1	XP_001085770.2
Bos taurus (cattle)	DLEC1	NP_001180127.1
Canis lupus familiaris (dog)	DLEC1	XP_005634305.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Dlec1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

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