MSRB1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	MSRB1
Entrez Gene ID	618441
Full Name	methionine sulfoxide reductase B1
Synonyms	SEPX1
Gene Type	protein-coding
Organism	Bos taurus(cattle)
Summary	The protein encoded by this gene belongs to the methionine-R-sulfoxide reductase B (MsrB) family. Members of this family function as repair enzymes that protect proteins from oxidative stress by catalyzing the reduction of methionine-R-sulfoxides to methionines. This protein is highly expressed in liver and kidney, and is localized to the nucleus and cytosol. It is the only member of the MsrB family that is a selenoprotein, containing a selenocysteine (Sec) residue at its active site. It also has the highest methionine-R-sulfoxide reductase activity compared to other members containing cysteine in place of Sec. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Oct 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001034810.2	NP_001029982.2	methionine-R-sulfoxide reductase B1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MSRB1	NP_057416.1
Pan troglodytes (chimpanzee)	SEPX1	NP_001108223.1
Canis lupus familiaris (dog)	SEPX1	NP_001108221.1
Rattus norvegicus (Norway rat)	Msrb1	NP_001037750.2
Xenopus tropicalis (tropical clawed frog)	msrb1	NP_001004972.2
	SEPX1	NP_001108222.1
Mus musculus (house mouse)	Msrb1	NP_038787.1
Gallus gallus (chicken)	MSRB1	NP_001129030.1
Danio rerio (zebrafish)	msrb1a	NP_840073.2
Bos taurus (cattle)	SEPX1	NP_001029982.2

Gene Ontology
Bibliography

Related articles in PubMed

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Characterization of mammalian selenoproteomes.
Kryukov GV, Castellano S, Novoselov SV, Lobanov AV, Zehtab O, Guig? R, Gladyshev VN
Science (New York, N.Y.)300(5624)1439-43(2003 May)

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Sonstegard TS, Capuco AV, White J, Van Tassell CP, Connor EE, Cho J, Sultana R, Shade L, Wray JE, Wells KD, Quackenbush J
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)

Generation of EST and cDNA microarray resources for the study of bovine immunobiology.
Yao J, Burton JL, Saama P, Sipkovsky S, Coussens PM
Acta veterinaria Scandinavica42(3)391-405(2001)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following MSRB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MSRB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa43993	NM_001034810.2 Latest version!	Bos taurus methionine sulfoxide reductase B1 (MSRB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MSRB1 ( NM_001034810.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001029982.2 Bos taurus methionine sulfoxide reductase B1 (MSRB1), mRNA.

Price & Availability↑

CloneID	OBa43993
Clone ID Related Accession (Same CDS sequence)	NM_001034810.2
Accession Version	NM_001034810.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-19
Organism	Bos taurus(cattle)
Product	methionine-R-sulfoxide reductase B1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CK836798.1 and BC105188.1. On Feb 22, 2008 this sequence version replaced NM_001034810.1. ##Evidence-Data-START## Transcript exon combination :: BC105188.1, DV791518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145418 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGTTCT GCAGCTTCTT CGGAGGCGAG ATTTTCCAGA ACCACTTTGA GCCGGGTATC 
TACGTGTGTG CCAAGTGTGG CTATGAGCTC TTCTCCAGCC GCTCCAAGTA CGCACACTCA 
TCCCCATGGC CGGCGTTCAC TGAGACCATC CATGCTGACA GTGTGGCCAA GCGGCCAGAG 
CACAATCGGC CTGGAGCCAT AAAGGTATCC TGTGGCAGGT GTGGCAACGG GCTGGGCCAT 
GAGTTCCTGA ACGATGGCCC CAAGCGCGGA CAGTCCCGCT TCTGAATTTT CAGCAGCTCA 
TTGAAGTTTA TCCCTAAAGC CGAAGAAACC TCCGCCTCCC AGGGACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001029982.2
CDS	155..505
Translation	MSFCSFFGGEIFQNHFEPGIYVCAKCGYELFSSRSKYAHSSPWPAFTETIHADSVAKRPEHNRPGAIKVSCGRCGNGLGHEFLNDGPKRGQSRF*IFSSSLKFIPKAEETSASQGQ

Target ORF information:

RefSeq Version	NM_001034810.2
Organism	Bos taurus(cattle)
Definition	Bos taurus methionine sulfoxide reductase B1 (MSRB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001034810.2

ORF Insert Sequence:

ATGTCGTTCT GCAGCTTCTT CGGAGGCGAG ATTTTCCAGA ACCACTTTGA GCCGGGTATC 
TACGTGTGTG CCAAGTGTGG CTATGAGCTC TTCTCCAGCC GCTCCAAGTA CGCACACTCA 
TCCCCATGGC CGGCGTTCAC TGAGACCATC CATGCTGACA GTGTGGCCAA GCGGCCAGAG 
CACAATCGGC CTGGAGCCAT AAAGGTATCC TGTGGCAGGT GTGGCAACGG GCTGGGCCAT 
GAGTTCCTGA ACGATGGCCC CAAGCGCGGA CAGTCCCGCT TCTGAATTTT CAGCAGCTCA 
TTGAAGTTTA TCCCTAAAGC CGAAGAAACC TCCGCCTCCC AGGGACAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Characterization of mammalian selenoproteomes.
Science (New York, N.Y.)300(5624)1439-43(2003 May)
Kryukov GV,Castellano S,Novoselov SV,Lobanov AV,Zehtab O,Guig? R,Gladyshev VN

Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Mammalian genome : official journal of the International Mammalian Genome Society13(7)373-9(2002 Jul)
Sonstegard TS,Capuco AV,White J,Van Tassell CP,Connor EE,Cho J,Sultana R,Shade L,Wray JE,Wells KD,Quackenbush J

Generation of EST and cDNA microarray resources for the study of bovine immunobiology.
Acta veterinaria Scandinavica42(3)391-405(2001)
Yao J,Burton JL,Saama P,Sipkovsky S,Coussens PM

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MSRB1 cDNA ORF clone, Bos taurus(cattle)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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