MSRB1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MSRB1
Entrez Gene ID	51734
Full Name	methionine sulfoxide reductase B1
Synonyms	HSPC270,SELR,SELX,SEPX1,SepR
General protein information	Preferred Names methionine sulfoxide reductase B1 Names methionine-R-sulfoxide reductase B1 selenoprotein R selenoprotein X, 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	The protein encoded by this gene belongs to the methionine-R-sulfoxide reductase B (MsrB) family. Members of this family function as repair enzymes that protect proteins from oxidative stress by catalyzing the reduction of methionine-R-sulfoxides to methionines. This protein is highly expressed in liver and kidney, and is localized to the nucleus and cytosol. It is the only member of the MsrB family that is a selenoprotein, containing a selenocysteine (Sec) residue at its active site. It also has the highest methionine-R-sulfoxide reductase activity compared to other members containing cysteine in place of Sec. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A pseudogene of this locus has been identified on chromosome 19. [provided by RefSeq, Aug 2017].
Disorder MIM:	606216

mRNA and Protein(s)

mRNA	Protein	Name
NM_016332.2	NP_057416.1	methionine-R-sulfoxide reductase B1
NM_016332.3	NP_057416.1	methionine-R-sulfoxide reductase B1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MSRB1	NP_057416.1
Pan troglodytes (chimpanzee)	SEPX1	NP_001108223.1
Canis lupus familiaris (dog)	SEPX1	NP_001108221.1
Rattus norvegicus (Norway rat)	Msrb1	NP_001037750.2
Xenopus tropicalis (tropical clawed frog)	msrb1	NP_001004972.2
	SEPX1	NP_001108222.1
Mus musculus (house mouse)	Msrb1	NP_038787.1
Gallus gallus (chicken)	MSRB1	NP_001129030.1
Danio rerio (zebrafish)	msrb1a	NP_840073.2
Bos taurus (cattle)	SEPX1	NP_001029982.2

Gene Ontology
Bibliography

Related articles in PubMed

Direct interaction between selenoprotein R and A?42.
Wang C, Chen P, He X, Peng Z, Chen S, Zhang R, Cheng J, Liu Q
Biochemical and biophysical research communications489(4)509-514(2017 08)

Selenoprotein X Gene Knockdown Aggravated H2O2-Induced Apoptosis in Liver LO2 Cells.
Tang J, Cao L, Li Q, Wang L, Jia G, Liu G, Chen X, Cai J, Shang H, Zhao H
Biological trace element research173(1)71-8(2016 Sep)

Selenoprotein R Protects Human Lens Epithelial Cells against D-Galactose-Induced Apoptosis by Regulating Oxidative Stress and Endoplasmic Reticulum Stress.
Dai J, Liu H, Zhou J, Huang K
International journal of molecular sciences17(2)231(2016 Feb)

Neutrophil granulocytes uniquely express, among human blood cells, high levels of Methionine-sulfoxide-reductase enzymes.
Achilli C, Ciana A, Rossi A, Balduini C, Minetti G
Journal of leukocyte biology83(1)181-9(2008 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

findings suggested that SelX played important roles in protecting LO2 cells against oxidative damage and reducing H2O2-induced apoptosis in liver cells.
Title: Selenoprotein X Gene Knockdown Aggravated H2O2-Induced Apoptosis in Liver LO2 Cells.

Selenoprotein R Protects Human Lens Epithelial Cells against D-Galactose-Induced Apoptosis by Regulating Oxidative Stress and Endoplasmic Reticulum Stress
Title: Selenoprotein R Protects Human Lens Epithelial Cells against D-Galactose-Induced Apoptosis by Regulating Oxidative Stress and Endoplasmic Reticulum Stress.

MsrB1 protected human lens epithelial cells against the peroxynitrite-induced F-actin disruption
Title: Effect of methionine sulfoxide reductase B1 (SelR) gene silencing on peroxynitrite-induced F-actin disruption in human lens epithelial cells.

Silencing the expression of the main Msr elements-MsrA, MsrB1, or MsrB2 exacerbates sensitivity toward oxidative stress.
Title: Proteome alteration in oxidative stress-sensitive methionine sulfoxide reductase-silenced HEK293 cells.

FRET and co-IP assays demonstrated that Clu interacted with beta-amyloid peptide, a pathological protein of AD, which suggested a potential effect of SelR and Abeta with the aid of Clu. The interaction between SelR and Clu provides a novel avenue for further study on the mechanism of SelR in AD prevention.
Title: Direct Interaction of Selenoprotein R with Clusterin and Its Possible Role in Alzheimer's Disease.

The following MSRB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MSRB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13452	NM_016332.3 Latest version!	Homo sapiens methionine sulfoxide reductase B1 (MSRB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu13452	NM_016332.2	Homo sapiens methionine sulfoxide reductase B1 (MSRB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MSRB1 ( NM_016332.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_057416.1 Homo sapiens methionine sulfoxide reductase B1 (MSRB1), mRNA.

Price & Availability↑

CloneID	OHu13452
Clone ID Related Accession (Same CDS sequence)	NM_016332.2 , NM_016332.3
Accession Version	NM_016332.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-07-09
Organism	Homo sapiens(Human)
Product	methionine-R-sulfoxide reductase B1
Comment

ATGTCGTTCT GCAGCTTCTT CGGGGGCGAG GTTTTCCAGA ATCACTTTGA ACCTGGCGTT 
TACGTGTGTG CCAAGTGTGG CTATGAGCTG TTCTCCAGCC GCTCGAAGTA TGCACACTCG 
TCTCCATGGC CGGCGTTCAC CGAGACCATT CACGCCGACA GCGTGGCCAA GCGTCCGGAG 
CACAATAGAT CTGAAGCCTT GAAGGTGTCC TGTGGCAAGT GTGGCAATGG GTTGGGCCAC 
GAGTTCCTGA ACGACGGCCC CAAGCCGGGG CAGTCCCGAT TCTGAATATT CAGCAGCTCG 
CTGAAGTTTG TCCCTAAAGG CAAAGAAACT TCTGCCTCCC AGGGTCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057416.1
Translation	MSFCSFFGGEVFQNHFEPGVYVCAKCGYELFSSRSKYAHSSPWPAFTETIHADSVAKRPEHNRSEALKVSCGKCGNGLGHEFLNDGPKPGQSRF*IFSSSLKFVPKGKETSASQGH

Target ORF information:

RefSeq Version	NM_016332.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens methionine sulfoxide reductase B1 (MSRB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016332.2

ORF Insert Sequence:

ATGTCGTTCT GCAGCTTCTT CGGGGGCGAG GTTTTCCAGA ATCACTTTGA ACCTGGCGTT 
TACGTGTGTG CCAAGTGTGG CTATGAGCTG TTCTCCAGCC GCTCGAAGTA TGCACACTCG 
TCTCCATGGC CGGCGTTCAC CGAGACCATT CACGCCGACA GCGTGGCCAA GCGTCCGGAG 
CACAATAGAT CTGAAGCCTT GAAGGTGTCC TGTGGCAAGT GTGGCAATGG GTTGGGCCAC 
GAGTTCCTGA ACGACGGCCC CAAGCCGGGG CAGTCCCGAT TCTGAATATT CAGCAGCTCG 
CTGAAGTTTG TCCCTAAAGG CAAAGAAACT TCTGCCTCCC AGGGTCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MSRB1 ( NM_016332.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057416.1 Homo sapiens methionine sulfoxide reductase B1 (MSRB1), mRNA.

Price & Availability↑

CloneID	OHu13452
Clone ID Related Accession (Same CDS sequence)	NM_016332.2 , NM_016332.3
Accession Version	NM_016332.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	methionine-R-sulfoxide reductase B1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CF134919.1, AF166124.1 and AI183441.1. On Oct 21, 2016 this sequence version replaced NM_016332.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK026128.1, SRR1163655.268474.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 10567350, 10608886 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGTTCT GCAGCTTCTT CGGGGGCGAG GTTTTCCAGA ATCACTTTGA ACCTGGCGTT 
TACGTGTGTG CCAAGTGTGG CTATGAGCTG TTCTCCAGCC GCTCGAAGTA TGCACACTCG 
TCTCCATGGC CGGCGTTCAC CGAGACCATT CACGCCGACA GCGTGGCCAA GCGTCCGGAG 
CACAATAGAT CTGAAGCCTT GAAGGTGTCC TGTGGCAAGT GTGGCAATGG GTTGGGCCAC 
GAGTTCCTGA ACGACGGCCC CAAGCCGGGG CAGTCCCGAT TCTGAATATT CAGCAGCTCG 
CTGAAGTTTG TCCCTAAAGG CAAAGAAACT TCTGCCTCCC AGGGTCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057416.1
CDS	171..521
Translation	MSFCSFFGGEVFQNHFEPGVYVCAKCGYELFSSRSKYAHSSPWPAFTETIHADSVAKRPEHNRSEALKVSCGKCGNGLGHEFLNDGPKPGQSRF*IFSSSLKFVPKGKETSASQGH

Target ORF information:

RefSeq Version	NM_016332.3
Organism	Homo sapiens(human)
Definition	Homo sapiens methionine sulfoxide reductase B1 (MSRB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016332.3

ORF Insert Sequence:

ATGTCGTTCT GCAGCTTCTT CGGGGGCGAG GTTTTCCAGA ATCACTTTGA ACCTGGCGTT 
TACGTGTGTG CCAAGTGTGG CTATGAGCTG TTCTCCAGCC GCTCGAAGTA TGCACACTCG 
TCTCCATGGC CGGCGTTCAC CGAGACCATT CACGCCGACA GCGTGGCCAA GCGTCCGGAG 
CACAATAGAT CTGAAGCCTT GAAGGTGTCC TGTGGCAAGT GTGGCAATGG GTTGGGCCAC 
GAGTTCCTGA ACGACGGCCC CAAGCCGGGG CAGTCCCGAT TCTGAATATT CAGCAGCTCG 
CTGAAGTTTG TCCCTAAAGG CAAAGAAACT TCTGCCTCCC AGGGTCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Direct interaction between selenoprotein R and A?42.
Biochemical and biophysical research communications489(4)509-514(2017 08)
Wang C,Chen P,He X,Peng Z,Chen S,Zhang R,Cheng J,Liu Q

Selenoprotein X Gene Knockdown Aggravated H2O2-Induced Apoptosis in Liver LO2 Cells.
Biological trace element research173(1)71-8(2016 Sep)
Tang J,Cao L,Li Q,Wang L,Jia G,Liu G,Chen X,Cai J,Shang H,Zhao H

Selenoprotein R Protects Human Lens Epithelial Cells against D-Galactose-Induced Apoptosis by Regulating Oxidative Stress and Endoplasmic Reticulum Stress.
International journal of molecular sciences17(2)231(2016 Feb)
Dai J,Liu H,Zhou J,Huang K

Neutrophil granulocytes uniquely express, among human blood cells, high levels of Methionine-sulfoxide-reductase enzymes.
Journal of leukocyte biology83(1)181-9(2008 Jan)
Achilli C,Ciana A,Rossi A,Balduini C,Minetti G

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MSRB1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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