Msrb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Msrb1
Entrez Gene ID	685059
Full Name	methionine sulfoxide reductase B1
Synonyms	Sepx1,selX
General protein information	Preferred Names methionine sulfoxide reductase B1 Names methionine-R-sulfoxide reductase B1 selenoprotein R selenoprotein X, 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q12
Summary	The protein encoded by this gene belongs to the methionine-R-sulfoxide reductase B (MsrB) family. Members of this family function as repair enzymes that protect proteins from oxidative stress by catalyzing the reduction of methionine-R-sulfoxides to methionines. This protein is highly expressed in liver and kidney, and is localized to the nucleus and cytosol. It is the only member of the MsrB family that is a selenoprotein, containing a selenocysteine (Sec) residue at its active site. It also has the highest methionine-R-sulfoxide reductase activity compared to other members containing cysteine in place of Sec. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. [provided by RefSeq, Oct 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001044285.3	NP_001037750.2	methionine-R-sulfoxide reductase B1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MSRB1	NP_057416.1
Pan troglodytes (chimpanzee)	SEPX1	NP_001108223.1
Canis lupus familiaris (dog)	SEPX1	NP_001108221.1
Rattus norvegicus (Norway rat)	Msrb1	NP_001037750.2
Xenopus tropicalis (tropical clawed frog)	msrb1	NP_001004972.2
	SEPX1	NP_001108222.1
Mus musculus (house mouse)	Msrb1	NP_038787.1
Gallus gallus (chicken)	MSRB1	NP_001129030.1
Danio rerio (zebrafish)	msrb1a	NP_840073.2
Bos taurus (cattle)	SEPX1	NP_001029982.2

Gene Ontology
Bibliography

Related articles in PubMed

Selenite and ebselen supplementation attenuates D-galactose-induced oxidative stress and increases expression of SELR and SEP15 in rat lens.
Dai J, Zhou J, Liu H, Huang K
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry21(8)1037-1046(2016 Dec)

MsrB1 and MICALs regulate actin assembly and macrophage function via reversible stereoselective methionine oxidation.
Lee BC, P?terfi Z, Hoffmann FW, Moore RE, Kaya A, Avanesov A, Tarrago L, Zhou Y, Weerapana E, Fomenko DE, Hoffmann PR, Gladyshev VN
Molecular cell51(3)397-404(2013 Aug)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

Diversity of protein and mRNA forms of mammalian methionine sulfoxide reductase B1 due to intronization and protein processing.
Liang X, Fomenko DE, Hua D, Kaya A, Gladyshev VN
PloS one5(7)e11497(2010 Jul)

Methionine sulfoxide reduction in mammals: characterization of methionine-R-sulfoxide reductases.
Kim HY, Gladyshev VN
Molecular biology of the cell15(3)1055-64(2004 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show that 15kD selenoprotein (SEP15) protein and selenoprotein R (SELR) protein expression was decreased in the lenses of galactose induced cataract.
Title: Selenite and ebselen supplementation attenuates D-galactose-induced oxidative stress and increases expression of SELR and SEP15 in rat lens.

The following Msrb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Msrb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa13124	NM_001044285.3 Latest version!	Rattus norvegicus methionine sulfoxide reductase B1 (Msrb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Msrb1 ( NM_001044285.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001037750.2 Rattus norvegicus methionine sulfoxide reductase B1 (Msrb1), mRNA.

Price & Availability↑

CloneID	ORa13124
Clone ID Related Accession (Same CDS sequence)	NM_001044285.3
Accession Version	NM_001044285.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Rattus norvegicus(Norway rat)
Product	methionine-R-sulfoxide reductase B1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from FQ220312.1, BC094309.1 and AI044339.1. On Oct 24, 2016 this sequence version replaced NM_001044285.2. ##Evidence-Data-START## Transcript exon combination :: FQ220312.1, BC094309.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGTTCT GCAGCTTCTT CGGAGGCGAG GTCTTCCAGA ATCACTTCGA GCCAGGCGTC 
TACGTGTGTG CCAAGTGCGG CTATGAGCTG TTCTCCAGTC GCTCGAAGTA CGCACACTCA 
TCCCCATGGC CAGCATTTAC TGAAACCATC CACGAGGACA GTGTGGCCAA GTGCCCTGAG 
AAAAACCGAC CGGAAGCTTT AAAGGTGTCC TGTGGCAAGT GTGGCAATGG GTTGGGCCAC 
GAGTTCCTCA ATGACGGCCC CAAGCGGGGA CAGTCGAGGT TCTGAATATT CAGCAGTTCT 
CTGAAGTTCA TCCCCAAAGG CAAAGAAGCT CCTGCCTCCC AGGGAGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001037750.2
CDS	82..432
Translation	MSFCSFFGGEVFQNHFEPGVYVCAKCGYELFSSRSKYAHSSPWPAFTETIHEDSVAKCPEKNRPEALKVSCGKCGNGLGHEFLNDGPKRGQSRF*IFSSSLKFIPKGKEAPASQGD

Target ORF information:

RefSeq Version	NM_001044285.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus methionine sulfoxide reductase B1 (Msrb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001044285.3

ORF Insert Sequence:

ATGTCGTTCT GCAGCTTCTT CGGAGGCGAG GTCTTCCAGA ATCACTTCGA GCCAGGCGTC 
TACGTGTGTG CCAAGTGCGG CTATGAGCTG TTCTCCAGTC GCTCGAAGTA CGCACACTCA 
TCCCCATGGC CAGCATTTAC TGAAACCATC CACGAGGACA GTGTGGCCAA GTGCCCTGAG 
AAAAACCGAC CGGAAGCTTT AAAGGTGTCC TGTGGCAAGT GTGGCAATGG GTTGGGCCAC 
GAGTTCCTCA ATGACGGCCC CAAGCGGGGA CAGTCGAGGT TCTGAATATT CAGCAGTTCT 
CTGAAGTTCA TCCCCAAAGG CAAAGAAGCT CCTGCCTCCC AGGGAGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenite and ebselen supplementation attenuates D-galactose-induced oxidative stress and increases expression of SELR and SEP15 in rat lens.
Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry21(8)1037-1046(2016 Dec)
Dai J,Zhou J,Liu H,Huang K

MsrB1 and MICALs regulate actin assembly and macrophage function via reversible stereoselective methionine oxidation.
Molecular cell51(3)397-404(2013 Aug)
Lee BC,P?terfi Z,Hoffmann FW,Moore RE,Kaya A,Avanesov A,Tarrago L,Zhou Y,Weerapana E,Fomenko DE,Hoffmann PR,Gladyshev VN

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

Diversity of protein and mRNA forms of mammalian methionine sulfoxide reductase B1 due to intronization and protein processing.
PloS one5(7)e11497(2010 Jul)
Liang X,Fomenko DE,Hua D,Kaya A,Gladyshev VN

Methionine sulfoxide reduction in mammals: characterization of methionine-R-sulfoxide reductases.
Molecular biology of the cell15(3)1055-64(2004 Mar)
Kim HY,Gladyshev VN

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Msrb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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