Msrb1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Msrb1
Entrez Gene ID	27361
Full Name	methionine sulfoxide reductase B1
Synonyms	D17Wsu82e,SELX,SelR,Sepr,Sepx1
General protein information	Preferred Names methionine sulfoxide reductase B1 Names methionine-R-sulfoxide reductase B1 selenoprotein R selenoprotein X 1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A3.3\|17 12.53 cM
Summary	The protein encoded by this gene belongs to the methionine-R-sulfoxide reductase B (MsrB) family. Members of this family function as repair enzymes that protect proteins from oxidative stress by catalyzing the reduction of methionine-R-sulfoxides to methionines. This protein is highly expressed in liver and kidney, and is localized to the nucleus and cytosol. It is the only member of the MsrB family that is a selenoprotein, containing a selenocysteine (Sec) residue at its active site. It also has the highest methionine-R-sulfoxide reductase activity compared to other members containing cysteine in place of Sec. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, Oct 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_013759.3	NP_038787.1	methionine-R-sulfoxide reductase B1
NM_001346668.1	NP_001333597.1	methionine-R-sulfoxide reductase B1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MSRB1	NP_057416.1
Pan troglodytes (chimpanzee)	SEPX1	NP_001108223.1
Canis lupus familiaris (dog)	SEPX1	NP_001108221.1
Rattus norvegicus (Norway rat)	Msrb1	NP_001037750.2
Xenopus tropicalis (tropical clawed frog)	msrb1	NP_001004972.2
	SEPX1	NP_001108222.1
Mus musculus (house mouse)	Msrb1	NP_038787.1
Gallus gallus (chicken)	MSRB1	NP_001129030.1
Danio rerio (zebrafish)	msrb1a	NP_840073.2
Bos taurus (cattle)	SEPX1	NP_001029982.2

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein MsrB1 deficiency exacerbates acetaminophen-induced hepatotoxicity via increased oxidative damage.
Kim KY, Kwak GH, Singh MP, Gladyshev VN, Kim HY
Archives of biochemistry and biophysics63469-75(2017 Nov)

Selenoprotein MsrB1 promotes anti-inflammatory cytokine gene expression in macrophages and controls immune response in vivo.
Lee BC, Lee SG, Choo MK, Kim JH, Lee HM, Kim S, Fomenko DE, Kim HY, Park JM, Gladyshev VN
Scientific reports7(1)5119(2017 Jul)

Methionine sulfoxide reductase B1 deficiency does not increase high-fat diet-induced insulin resistance in mice.
Heo JY, Cha HN, Kim KY, Lee E, Kim SJ, Kim YW, Kim JY, Lee IK, Gladyshev VN, Kim HY, Park SY
Free radical research51(1)24-37(2017 Jan)

Diversity of protein and mRNA forms of mammalian methionine sulfoxide reductase B1 due to intronization and protein processing.
Liang X, Fomenko DE, Hua D, Kaya A, Gladyshev VN
PloS one5(7)e11497(2010 Jul)

NMR assignments of 1H, 13C and 15N spectra of methionine sulfoxide reductase B1 from Mus musculus.
Sal LS, Aachmann FL, Kim HY, Gladyshev VN, Dikiy A
Biomolecular NMR assignments1(1)131-3(2007 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Collectively, the results of this study suggest that selenoprotein MsrB1 plays a protective role against APAP-induced hepatotoxicity via its antioxidative function.
Title: Selenoprotein MsrB1 deficiency exacerbates acetaminophen-induced hepatotoxicity via increased oxidative damage.

MsrB1 deficiency does not increase high-fat diet-induced insulin resistance in mice.
Title: Methionine sulfoxide reductase B1 deficiency does not increase high-fat diet-induced insulin resistance in mice.

study identified the regulatory role of MsrB1 as a Mical antagonist in orchestrating actin dynamics and macrophage function
Title: MsrB1 and MICALs regulate actin assembly and macrophage function via reversible stereoselective methionine oxidation.

MsrB1/Trx complex was studied in attempt to understand MsrB1 reduction mechanism. Using NMR data, molecular mechanics, protein docking & molecular dynamics simulations, found that intermediate MsrB1/Trx complex is stabilized by interprotein beta-layer.
Title: Structural insights into interaction between mammalian methionine sulfoxide reductase B1 and thioredoxin.

Analyses of fruit flies that do not express selenoproteins or express the mouse selenoprotein, methionine sulfoxide reductase B1, reveal a role of selenoproteins in stress resistance.
Title: Analyses of fruit flies that do not express selenoproteins or express the mouse selenoprotein, methionine sulfoxide reductase B1, reveal a role of selenoproteins in stress resistance.

The following Msrb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Msrb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu18827	NM_013759.3 Latest version!	Mus musculus methionine sulfoxide reductase B1 (Msrb1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OMu18827	NM_001346668.1 Latest version!	Mus musculus methionine sulfoxide reductase B1 (Msrb1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Msrb1 ( NM_013759.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_038787.1 Mus musculus methionine sulfoxide reductase B1 (Msrb1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu18827
Clone ID Related Accession (Same CDS sequence)	NM_013759.3 , NM_001346668.1
Accession Version	NM_013759.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Mus musculus(house mouse)
Product	methionine-R-sulfoxide reductase B1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY034747.1, BC090646.1 and BI133579.1. On Oct 24, 2016 this sequence version replaced NM_013759.2. Transcript Variant: This variant (1) represents the predominant 5-exon transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF195142.1, BC090646.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 10567350 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGTTCT GCAGCTTCTT CGGAGGCGAG GTTTTCCAGA ATCACTTCGA GCCAGGTGTC 
TACGTGTGTG CCAAGTGCAG CTATGAGCTG TTCTCCAGTC ACTCGAAGTA CGCACACTCA 
TCCCCGTGGC CAGCGTTCAC TGAAACCATC CACCCAGACA GTGTGACCAA GTGCCCTGAG 
AAAAACCGAC CAGAAGCTTT AAAGGTGTCC TGTGGCAAGT GTGGCAATGG GTTGGGCCAC 
GAGTTCCTGA ATGATGGCCC CAAGCGGGGA CAATCAAGAT TCTGAATATT TAGCAGCTCA 
CTGAAGTTCG TCCCTAAAGG CAAAGAAGCT GCTGCCTCCC AGGGGCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_038787.1
CDS	116..466
Translation	MSFCSFFGGEVFQNHFEPGVYVCAKCSYELFSSHSKYAHSSPWPAFTETIHPDSVTKCPEKNRPEALKVSCGKCGNGLGHEFLNDGPKRGQSRF*IFSSSLKFVPKGKEAAASQGH

Target ORF information:

RefSeq Version	NM_013759.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus methionine sulfoxide reductase B1 (Msrb1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013759.3

ORF Insert Sequence:

ATGTCGTTCT GCAGCTTCTT CGGAGGCGAG GTTTTCCAGA ATCACTTCGA GCCAGGTGTC 
TACGTGTGTG CCAAGTGCAG CTATGAGCTG TTCTCCAGTC ACTCGAAGTA CGCACACTCA 
TCCCCGTGGC CAGCGTTCAC TGAAACCATC CACCCAGACA GTGTGACCAA GTGCCCTGAG 
AAAAACCGAC CAGAAGCTTT AAAGGTGTCC TGTGGCAAGT GTGGCAATGG GTTGGGCCAC 
GAGTTCCTGA ATGATGGCCC CAAGCGGGGA CAATCAAGAT TCTGAATATT TAGCAGCTCA 
CTGAAGTTCG TCCCTAAAGG CAAAGAAGCT GCTGCCTCCC AGGGGCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Msrb1 ( NM_001346668.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001333597.1 Mus musculus methionine sulfoxide reductase B1 (Msrb1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu18827
Clone ID Related Accession (Same CDS sequence)	NM_013759.3 , NM_001346668.1
Accession Version	NM_001346668.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-22
Organism	Mus musculus(house mouse)
Product	methionine-R-sulfoxide reductase B1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BY034747.1, AK003112.1, AK007404.1 and BI133579.1. Transcript Variant: This variant (2) is a 4-exon transcript that is alternatively spliced at the 3' end compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK003112.1, BI415369.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849374, SAMN00849375 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: PMID: 10567350 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCGTTCT GCAGCTTCTT CGGAGGCGAG GTTTTCCAGA ATCACTTCGA GCCAGGTGTC 
TACGTGTGTG CCAAGTGCAG CTATGAGCTG TTCTCCAGTC ACTCGAAGTA CGCACACTCA 
TCCCCGTGGC CAGCGTTCAC TGAAACCATC CACCCAGACA GTGTGACCAA GTGCCCTGAG 
AAAAACCGAC CAGAAGCTTT AAAGGTGTCC TGTGGCAAGT GTGGCAATGG GTTGGGCCAC 
GAGTTCCTGA ATGATGGCCC CAAGCGGGGA CAATCAAGAT TCTGAATATT TAGCAGCTCA 
CTGAAGTTCG TCCCTAAAGG CAAAGAAGCT GCTGCCTCCC AGGGGCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001333597.1
CDS	116..466
Translation	MSFCSFFGGEVFQNHFEPGVYVCAKCSYELFSSHSKYAHSSPWPAFTETIHPDSVTKCPEKNRPEALKVSCGKCGNGLGHEFLNDGPKRGQSRF*IFSSSLKFVPKGKEAAASQGH

Target ORF information:

RefSeq Version	NM_001346668.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus methionine sulfoxide reductase B1 (Msrb1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001346668.1

ORF Insert Sequence:

ATGTCGTTCT GCAGCTTCTT CGGAGGCGAG GTTTTCCAGA ATCACTTCGA GCCAGGTGTC 
TACGTGTGTG CCAAGTGCAG CTATGAGCTG TTCTCCAGTC ACTCGAAGTA CGCACACTCA 
TCCCCGTGGC CAGCGTTCAC TGAAACCATC CACCCAGACA GTGTGACCAA GTGCCCTGAG 
AAAAACCGAC CAGAAGCTTT AAAGGTGTCC TGTGGCAAGT GTGGCAATGG GTTGGGCCAC 
GAGTTCCTGA ATGATGGCCC CAAGCGGGGA CAATCAAGAT TCTGAATATT TAGCAGCTCA 
CTGAAGTTCG TCCCTAAAGG CAAAGAAGCT GCTGCCTCCC AGGGGCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein MsrB1 deficiency exacerbates acetaminophen-induced hepatotoxicity via increased oxidative damage.
Archives of biochemistry and biophysics63469-75(2017 Nov)
Kim KY,Kwak GH,Singh MP,Gladyshev VN,Kim HY

Selenoprotein MsrB1 promotes anti-inflammatory cytokine gene expression in macrophages and controls immune response in vivo.
Scientific reports7(1)5119(2017 Jul)
Lee BC,Lee SG,Choo MK,Kim JH,Lee HM,Kim S,Fomenko DE,Kim HY,Park JM,Gladyshev VN

Methionine sulfoxide reductase B1 deficiency does not increase high-fat diet-induced insulin resistance in mice.
Free radical research51(1)24-37(2017 Jan)
Heo JY,Cha HN,Kim KY,Lee E,Kim SJ,Kim YW,Kim JY,Lee IK,Gladyshev VN,Kim HY,Park SY

Diversity of protein and mRNA forms of mammalian methionine sulfoxide reductase B1 due to intronization and protein processing.
PloS one5(7)e11497(2010 Jul)
Liang X,Fomenko DE,Hua D,Kaya A,Gladyshev VN

NMR assignments of 1H, 13C and 15N spectra of methionine sulfoxide reductase B1 from Mus musculus.
Biomolecular NMR assignments1(1)131-3(2007 Jul)
Sal LS,Aachmann FL,Kim HY,Gladyshev VN,Dikiy A

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Msrb1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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