msrb1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	msrb1a
Entrez Gene ID	352916
Full Name	methionine sulfoxide reductase B1a
Synonyms	MsrB,MsrB1-A,SePR,SelX-A,cb687,selx,sepx1,sepx1a,wu:fa03e09
General protein information	Preferred Names methionine sulfoxide reductase B1a Names methionine-R-sulfoxide reductase B1-A methionine-R-sulfoxide reductase B1 msrB1 selenoprotein X, 1 selenoprotein X, 1a selenoprotein X-A
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	The protein encoded by this gene belongs to the methionine-R-sulfoxide reductase B (MsrB) family. Members of this family function as repair enzymes that protect proteins from oxidative stress by catalyzing the reduction of methionine-R-sulfoxides to methionines. This protein is highly expressed in liver and kidney, and is localized to the nucleus and cytosol. It is the only member of the MsrB family that is a selenoprotein, containing a selenocysteine (Sec) residue at its active site. It also has the highest methionine-R-sulfoxide reductase activity compared to other members containing cysteine in place of Sec. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon, rather than as a stop signal. A paralog of this gene (msrb1b) exists on chromosome 12. [provided by RefSeq, Oct 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_178288.5	NP_840073.2	methionine-R-sulfoxide reductase B1-A

Pathways from BioSystems

Homologies

Homo sapiens (human)	MSRB1	NP_057416.1
Pan troglodytes (chimpanzee)	SEPX1	NP_001108223.1
Canis lupus familiaris (dog)	SEPX1	NP_001108221.1
Rattus norvegicus (Norway rat)	Msrb1	NP_001037750.2
Xenopus tropicalis (tropical clawed frog)	msrb1	NP_001004972.2
	SEPX1	NP_001108222.1
Mus musculus (house mouse)	Msrb1	NP_038787.1
Gallus gallus (chicken)	MSRB1	NP_001129030.1
Danio rerio (zebrafish)	msrb1a	NP_840073.2
Bos taurus (cattle)	SEPX1	NP_001029982.2

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of genetic loss-of-function of Fus in zebrafish.
Lebedeva S, de Jesus Domingues AM, Butter F, Ketting RF
RNA biology14(1)29-35(2017 01)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Penglase S, Hamre K, Ellingsen S
Free radical biology & medicine7595-104(2014 Oct)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

Evolution of gene function and regulatory control after whole-genome duplication: comparative analyses in vertebrates.
Kassahn KS, Dang VT, Wilkins SJ, Perkins AC, Ragan MA
Genome research19(8)1404-18(2009 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following msrb1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the msrb1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa36217	NM_178288.5 Latest version!	Danio rerio methionine sulfoxide reductase B1a (msrb1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

msrb1a ( NM_178288.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_840073.2 Danio rerio methionine sulfoxide reductase B1a (msrb1a), mRNA.

Price & Availability↑

CloneID	ODa36217
Clone ID Related Accession (Same CDS sequence)	NM_178288.5
Accession Version	NM_178288.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Danio rerio(zebrafish)
Product	methionine-R-sulfoxide reductase B1-A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL772362.8, EH536771.1, EH572189.1 and EH525837.1. On Nov 2, 2016 this sequence version replaced NM_178288.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC067380.1, GFIL01026956.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476743, SAMEA4476745 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTTTCT GTTCGTTCTC TGGAGGGGAG ATTTATAAAG ATCATTTTGA GAGCGGCATG 
TATGTGTGTG CTCAGTGTGG ATACGAGCTC TTCTCCAGCA GATCCAAATA TGAGCACTCG 
TCCCCCTGGC CAGCCTTCAC TGAAACCATC CATGAAGACA GCGTGTCTAA ACAGGAGGAA 
AGATGGGGCG CCTACAAGGT CAGATGTGGA AAGTGTGGAA ATGGTCTTGG CCATGAGTTT 
GTGAATGACG GACCCAAACA CGGCCTCTCA CGCTTCTGAA TATTTAGCAG CTCTCTGAAG 
TTCATTCCTA AAGTGAAGAA TGAGCAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_840073.2
CDS	133..465
Translation	MSFCSFSGGEIYKDHFESGMYVCAQCGYELFSSRSKYEHSSPWPAFTETIHEDSVSKQEERWGAYKVRCGKCGNGLGHEFVNDGPKHGLSRF*IFSSSLKFIPKVKNEQQ

Target ORF information:

RefSeq Version	NM_178288.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio methionine sulfoxide reductase B1a (msrb1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178288.5

ORF Insert Sequence:

ATGTCTTTCT GTTCGTTCTC TGGAGGGGAG ATTTATAAAG ATCATTTTGA GAGCGGCATG 
TATGTGTGTG CTCAGTGTGG ATACGAGCTC TTCTCCAGCA GATCCAAATA TGAGCACTCG 
TCCCCCTGGC CAGCCTTCAC TGAAACCATC CATGAAGACA GCGTGTCTAA ACAGGAGGAA 
AGATGGGGCG CCTACAAGGT CAGATGTGGA AAGTGTGGAA ATGGTCTTGG CCATGAGTTT 
GTGAATGACG GACCCAAACA CGGCCTCTCA CGCTTCTGAA TATTTAGCAG CTCTCTGAAG 
TTCATTCCTA AAGTGAAGAA TGAGCAACAG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of genetic loss-of-function of Fus in zebrafish.
RNA biology14(1)29-35(2017 01)
Lebedeva S,de Jesus Domingues AM,Butter F,Ketting RF

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Free radical biology & medicine7595-104(2014 Oct)
Penglase S,Hamre K,Ellingsen S

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

Evolution of gene function and regulatory control after whole-genome duplication: comparative analyses in vertebrates.
Genome research19(8)1404-18(2009 Aug)
Kassahn KS,Dang VT,Wilkins SJ,Perkins AC,Ragan MA

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msrb1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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