APAF1 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	APAF1
Entrez Gene ID	537782
Full Name	apoptotic peptidase activating factor 1
General protein information	Preferred Names apoptotic peptidase activating factor 1 Names apoptotic protease-activating factor 1
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010805196.2	XP_010803498.1	apoptotic protease-activating factor 1 isoform X2
XM_010805198.3	XP_010803500.1	apoptotic protease-activating factor 1 isoform X3
XM_015471110.2	XP_015326596.1	apoptotic protease-activating factor 1 isoform X1
NM_001191507.1	NP_001178436.1	apoptotic protease-activating factor 1
XM_015471110.1	XP_015326596.1	apoptotic protease-activating factor 1 isoform X1
XM_010805198.2	XP_010803500.1	apoptotic protease-activating factor 1 isoform X3
XM_010805196.3	XP_010803498.1	apoptotic protease-activating factor 1 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	APAF1	NP_863651.1
Canis lupus familiaris (dog)	APAF1	XP_003432082.1
Bos taurus (cattle)	APAF1	NP_001178436.1
Xenopus tropicalis (tropical clawed frog)	apaf1	NP_001106546.1
Gallus gallus (chicken)	APAF1	XP_416167.3
Danio rerio (zebrafish)	apaf1	NP_571683.1
Pan troglodytes (chimpanzee)	APAF1	XP_003313928.1
	APAF1	XP_001086945.1
Mus musculus (house mouse)	Apaf1	NP_033814.2
Rattus norvegicus (Norway rat)	Apaf1	NP_076469.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Smith TP, Grosse WM, Freking BA, Roberts AJ, Stone RT, Casas E, Wray JE, White J, Cho J, Fahrenkrug SC, Bennett GL, Heaton MP, Laegreid WW, Rohrer GA, Chitko-McKown CG, Pertea G, Holt I, Karamycheva S, Liang F, Quackenbush J, Keele JW
Genome research11(4)626-30(2001 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following APAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa39754	NM_001191507.1 Latest version!	Bos taurus apoptotic peptidase activating factor 1 (APAF1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa19473	XM_015471110.2 Latest version!	Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa53779	XM_010805196.3 Latest version!	Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa53781	XM_010805198.3 Latest version!	Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
OBa19473	XM_015471110.1	Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa53781	XM_010805198.2	Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
OBa53779	XM_010805196.2	Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APAF1 ( NM_001191507.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178436.1 Bos taurus apoptotic peptidase activating factor 1 (APAF1), mRNA.

Price & Availability↑

CloneID	OBa39754
Clone ID Related Accession (Same CDS sequence)	NM_001191507.1
Accession Version	NM_001191507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	apoptotic protease-activating factor 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000005.1 and BE663934.1. On Apr 28, 2011 this sequence version replaced XM_002704359.1. Sequence Note: This RefSeq record was created from transcripts of different breeds and genomic sequence data because no single transcript from the same breed was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and orthologous data. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN03145413, SAMN03145415 [ECO:0000350] ##Evidence-Data-END##

ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCCTTGGA AAGGGACATC 
AAAACATCCT ACATTATGGA TCACATGATT AGTAATGGAG TTTTAACAGT GTCAGAGGAG 
GAAAAAGTGA AAAATGAGCC CACCCAACGG CAACGAGCAG CTATGCTAAT TAAAATGATA 
CTTAAAAAAG ATAATCATTC CTATATATCG TTCTACAATG CTCTACTTCA TGAAGGTTAC 
AAAGATCTTG CTGCCCTTCT GCATGGTGGC ATTCCTGTTA TCTCTTCTTC CGATGTAAGG 
ACAGTCCTGT GTGAAGGTGG AGTACCACAG AGGCCCGTTG TTTTTGTTAC TAGGAAGAAG 
CTAGTGAATG CAATTCAGCA GAAGCTCTCC AAACTGAATG GTGAACCAGG ATGGGTCACC 
ATATATGGGA TGGCAGGTTG TGGGAAGTCT GTATTAGCTG CAGAAGCTGT TCGAGATCAT 
TCTTTCCTAG AAGATTGTTT CCCAGGAGGC GTGCATTGGG TGTCAGTTGG GAAACAGGAC 
AAATCCGGGC TTCTGATGAA ACTACAGAAC CTTTGTGCAC GATTGGATCA GGATGAGAGC 
TTCTCCCAGA GGCTTCCACT TAATATTGAA GAGGCTAAAG ACCGTCTCCG CATTCTGATG 
CTGCGCAAAC ATCCAAGGTC TTTATTGATC TTGGATGATA TTTGGGATCC TTGGGTGTTA 
AAAGCTTTTG ACAATCAGTG TCAAATTCTT CTCACAACTA GAGATAAGAG TGTTACAGAT 
TCAGTGATGG GTCCTAAATA TGTAGTGCCT GTGGAGAGTG GTTTAGGAAA AGAAAAAGGA 
CTTGAAATTT TATCCCTTTT TGTTAATATG AAGAAAGCAG ATTTGCCAGA ACAAGCTCAC 
AGTATTATAC AAGAATGTAA AGGTTCCCCT CTTGTAGTAT CTTTAATTGG TGCACTTTTA 
CGAGATTTTC CCAACCGCTG GGAGTACTAC CTCAGGCAAC TTCAGAATAA GCAATTTAAG 
AGGATAAGAA AATCTTCATC TTATGACTAT GAGGCTCTAG ATGAAGCCAT GTCTATAAGT 
GTTGAAATGC TCAGAGAAGA CATCAAAGAT TATTACACAG ATCTTTCCAT CTTACAGAAA 
GATGTTAAGG TGCCTACAAA GGTGTTATGT ATCCTCTGGG ACTTGGAAAC GGAAGAAGTT 
GAAGACATAT TACAGGAATT TGTTAATAAG TCTCTCTTGT TCTGTGACCG GAATGGAAAA 
TCATTTCTTT ATTATTTACA TGATCTGCAA GTAGATTTCC TTGCTGAAAA GAATCGCAGC 
CAGCTTCAGG ATCTCCACAA GAAGATGGTC ACTCAGTTCC AGAGACATCA CCAGCTATAT 
TCTCTTTCAC CAGATCAGGA GGATTGCATG TATTGGTACA GTTTTCTGGC CTATCACATG 
GCCAGTGCTA ATATGCACAA AGAACTGTGT GCTTTAATGT TTTCCCTGGA TTGGATTAAA 
GCAAAAACGG AACTTGTAGG CCCTGCTCAT CTGATTCATG AATTTGTGGA ATACAGGCAT 
ATATTGGATG AAAAGGATGG TATAGACTGT GAGAATTTCC AGGAGTTTTT ATCTTTAAAT 
GGACATCTTC TTGGACGACA GCCATTTCCT AATATTGTGC AACTGGGCCT CTGTGAACTG 
GAAACTTCAG AGGTTTATCG GCAAGCTAAG CTGCAGGCCA AGCAGGAGGT CGATAACGGA 
ATGCTTTACC TGGAGTGGGT AAACAAAAAA AACATGAAGA ACCTCTCCCG CTTGGTTGTC 
CGCCCCCATA CAGATGCTGT TTACCATGCT TGCTTTTCTG AAGATGGTCA AAGAATAGCG 
TCTTGTGGAG CTGATAAAAC TTTACAGGTA TTCAAAGCTG AAACAGGAGA GAAACTTCTA 
GAAATCAAGG CTCATGAGGA TGAAGTGCTT TGCTGTGCAT TCTCTGCAGA TGACAAATTT 
ATAGCAACCT GCTCAGTGGA TAAAAAAGTG AAGATTTGGA ATTCAATGAC TGGGGAACTA 
GTGCGTATCT ATGATGAGCA CTCAGAGCAA GTCAATTGCT GCCACTTCAC CAACAATAGT 
AATCACCTTC TCTTAGCCAC TGGTTCAAAT GACTACTTCC TTAAACTCTG GGATCTGAAT 
CAAGAAGAAT GTCGAAATAC CATGTTTGGC CATACAAATT CGGTCAGTCA CTGCAGATTT 
TCACCAGATG ATCAGGTTTT GGCCAGTTGT TCAGCTGATG GAACATTAAA GCTTTGGGAT 
GTGAAATCTG CAAATGAGAG GAAAAGCATT AATGTGAAGC AATTCTTCCT GAATTCAGAG 
GAGCCTCAGG AGGATCTGGA AGTGATAGTG AAATGCTGTT CGTGGTCTGC TGATGGTGCT 
AAGATAATGG TGGCTGCGAA AAATAAAGTC TTTCTTTTTG ACATTCACTC CAGTGGCCTG 
TTGGCAGAAA TGCACATGGG CCATCATAGC GCTGTCCAGT ACTGTGACTT CTCCCCCCAC 
AACCATTTGG CAGTGGTGGC TTTGTCCCAG TACTGTGTGG AGTTGTGGAG TATGGACTCA 
GGTTTAAAAG TAGCTGATTG CAGAGGACAT TTAAGTTGGG TCCATTGTGT GATGTTTTCT 
CCTGATGGAT CATCGTTTTT GACATCTTCT GATGACCAGA CAATCAGGCT CTGGGAGACA 
AAGAAAGTAT GTAAGAACTC TGCTACAGTG TTAAAGCAAG AAATAGATGT TGTGTTTCAA 
GAAAATGAAG TGACGGTTCT TGCAGTTGAC AATATAAGAC GTCTGCAACT CATTAATGGA 
AACACAGGTC AGATTGATTA CCTAACTGAA GCTCAAGTGA GTTGCTGTTG TTTAAGTCCA 
CATCTTCAGT ACCTTGCATT CGGAGGTGAA GATGGAGCCA TTCAGATTTT AGAACTCCTG 
AACAACAGAA TCTTCCAATC CAGAAATGGG CACAAGACCA CTGTTCGGCA CATCCAGTTC 
ACAGCTGATG GAAAGACTCT TATCTCAAGC TCTGATGATT CTGCAGTTCA GATATGGAAC 
TGGCAATCAG AGGACCCTTT CTTTCTCCAA GCCCACCAGG AAACAGTGAA AGACTTCAGA 
CTCTTGAAAA ATTCAAGACT TCTTTCCTGG TCATTTGATG GAACAGTAAA GGTATGGAAT 
ATTATTACCA GAAGAATAGA AAAAGATTTC GTGTGTCACC AGGATACAGT TCTTTCTTGT 
GATATTTCCT CTGATGCTAC TAAGTTTTCT TCTACCTCTG CTGACAAGAC TGCAAAGATT 
TGGAGTTTTG AACTCCTTTC TCCTCTTCAT GAATTGAGAG GCCACAAAGG CTGTGTGCGC 
TGCTCTTCCT TCTCTGTGAA CAATACCTTG TTGGCGACTG GAGACGACAA TGGAGAAATC 
AGGATATGGA ATGTCTCAAA CGGTGAGCTT CTTCATTTGT GTGCTCCGAT TTCAGTAGAA 
GAAGGAGCTG CTACTCATGG AGGCTGGGTG ACCGACCTTT GCTTTTCTCC AGACACCAAA 
ATGCTTGTTT CTGCTGGAGG ATATCTCAAG TGGTGGAATG TTGTCACTGG GGAATCCTCA 
CAGACCTTCT ACACAAACGG AACCAATCTT AAGAAAATAC ACGTGTCCCC TGACTTCAAA 
ACATATGTGA CTGTTGATAA TCTTGGTATT TTATATATTC TAAAGATTTT AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178436.1
CDS	1..3717
Translation	MDAKARNCLLQHREALERDIKTSYIMDHMISNGVLTVSEEEKVKNEPTQRQRAAMLIKMILKKDNHSYISFYNALLHEGYKDLAALLHGGIPVISSSDVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLNGEPGWVTIYGMAGCGKSVLAAEAVRDHSFLEDCFPGGVHWVSVGKQDKSGLLMKLQNLCARLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDIWDPWVLKAFDNQCQILLTTRDKSVTDSVMGPKYVVPVESGLGKEKGLEILSLFVNMKKADLPEQAHSIIQECKGSPLVVSLIGALLRDFPNRWEYYLRQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDLETEEVEDILQEFVNKSLLFCDRNGKSFLYYLHDLQVDFLAEKNRSQLQDLHKKMVTQFQRHHQLYSLSPDQEDCMYWYSFLAYHMASANMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDGIDCENFQEFLSLNGHLLGRQPFPNIVQLGLCELETSEVYRQAKLQAKQEVDNGMLYLEWVNKKNMKNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSADDKFIATCSVDKKVKIWNSMTGELVRIYDEHSEQVNCCHFTNNSNHLLLATGSNDYFLKLWDLNQEECRNTMFGHTNSVSHCRFSPDDQVLASCSADGTLKLWDVKSANERKSINVKQFFLNSEEPQEDLEVIVKCCSWSADGAKIMVAAKNKVFLFDIHSSGLLAEMHMGHHSAVQYCDFSPHNHLAVVALSQYCVELWSMDSGLKVADCRGHLSWVHCVMFSPDGSSFLTSSDDQTIRLWETKKVCKNSATVLKQEIDVVFQENEVTVLAVDNIRRLQLINGNTGQIDYLTEAQVSCCCLSPHLQYLAFGGEDGAIQILELLNNRIFQSRNGHKTTVRHIQFTADGKTLISSSDDSAVQIWNWQSEDPFFLQAHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITRRIEKDFVCHQDTVLSCDISSDATKFSSTSADKTAKIWSFELLSPLHELRGHKGCVRCSSFSVNNTLLATGDDNGEIRIWNVSNGELLHLCAPISVEEGAATHGGWVTDLCFSPDTKMLVSAGGYLKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILKILE

Target ORF information:

RefSeq Version	NM_001191507.1
Organism	Bos taurus(cattle)
Definition	Bos taurus apoptotic peptidase activating factor 1 (APAF1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191507.1

ORF Insert Sequence:

ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCCTTGGA AAGGGACATC 
AAAACATCCT ACATTATGGA TCACATGATT AGTAATGGAG TTTTAACAGT GTCAGAGGAG 
GAAAAAGTGA AAAATGAGCC CACCCAACGG CAACGAGCAG CTATGCTAAT TAAAATGATA 
CTTAAAAAAG ATAATCATTC CTATATATCG TTCTACAATG CTCTACTTCA TGAAGGTTAC 
AAAGATCTTG CTGCCCTTCT GCATGGTGGC ATTCCTGTTA TCTCTTCTTC CGATGTAAGG 
ACAGTCCTGT GTGAAGGTGG AGTACCACAG AGGCCCGTTG TTTTTGTTAC TAGGAAGAAG 
CTAGTGAATG CAATTCAGCA GAAGCTCTCC AAACTGAATG GTGAACCAGG ATGGGTCACC 
ATATATGGGA TGGCAGGTTG TGGGAAGTCT GTATTAGCTG CAGAAGCTGT TCGAGATCAT 
TCTTTCCTAG AAGATTGTTT CCCAGGAGGC GTGCATTGGG TGTCAGTTGG GAAACAGGAC 
AAATCCGGGC TTCTGATGAA ACTACAGAAC CTTTGTGCAC GATTGGATCA GGATGAGAGC 
TTCTCCCAGA GGCTTCCACT TAATATTGAA GAGGCTAAAG ACCGTCTCCG CATTCTGATG 
CTGCGCAAAC ATCCAAGGTC TTTATTGATC TTGGATGATA TTTGGGATCC TTGGGTGTTA 
AAAGCTTTTG ACAATCAGTG TCAAATTCTT CTCACAACTA GAGATAAGAG TGTTACAGAT 
TCAGTGATGG GTCCTAAATA TGTAGTGCCT GTGGAGAGTG GTTTAGGAAA AGAAAAAGGA 
CTTGAAATTT TATCCCTTTT TGTTAATATG AAGAAAGCAG ATTTGCCAGA ACAAGCTCAC 
AGTATTATAC AAGAATGTAA AGGTTCCCCT CTTGTAGTAT CTTTAATTGG TGCACTTTTA 
CGAGATTTTC CCAACCGCTG GGAGTACTAC CTCAGGCAAC TTCAGAATAA GCAATTTAAG 
AGGATAAGAA AATCTTCATC TTATGACTAT GAGGCTCTAG ATGAAGCCAT GTCTATAAGT 
GTTGAAATGC TCAGAGAAGA CATCAAAGAT TATTACACAG ATCTTTCCAT CTTACAGAAA 
GATGTTAAGG TGCCTACAAA GGTGTTATGT ATCCTCTGGG ACTTGGAAAC GGAAGAAGTT 
GAAGACATAT TACAGGAATT TGTTAATAAG TCTCTCTTGT TCTGTGACCG GAATGGAAAA 
TCATTTCTTT ATTATTTACA TGATCTGCAA GTAGATTTCC TTGCTGAAAA GAATCGCAGC 
CAGCTTCAGG ATCTCCACAA GAAGATGGTC ACTCAGTTCC AGAGACATCA CCAGCTATAT 
TCTCTTTCAC CAGATCAGGA GGATTGCATG TATTGGTACA GTTTTCTGGC CTATCACATG 
GCCAGTGCTA ATATGCACAA AGAACTGTGT GCTTTAATGT TTTCCCTGGA TTGGATTAAA 
GCAAAAACGG AACTTGTAGG CCCTGCTCAT CTGATTCATG AATTTGTGGA ATACAGGCAT 
ATATTGGATG AAAAGGATGG TATAGACTGT GAGAATTTCC AGGAGTTTTT ATCTTTAAAT 
GGACATCTTC TTGGACGACA GCCATTTCCT AATATTGTGC AACTGGGCCT CTGTGAACTG 
GAAACTTCAG AGGTTTATCG GCAAGCTAAG CTGCAGGCCA AGCAGGAGGT CGATAACGGA 
ATGCTTTACC TGGAGTGGGT AAACAAAAAA AACATGAAGA ACCTCTCCCG CTTGGTTGTC 
CGCCCCCATA CAGATGCTGT TTACCATGCT TGCTTTTCTG AAGATGGTCA AAGAATAGCG 
TCTTGTGGAG CTGATAAAAC TTTACAGGTA TTCAAAGCTG AAACAGGAGA GAAACTTCTA 
GAAATCAAGG CTCATGAGGA TGAAGTGCTT TGCTGTGCAT TCTCTGCAGA TGACAAATTT 
ATAGCAACCT GCTCAGTGGA TAAAAAAGTG AAGATTTGGA ATTCAATGAC TGGGGAACTA 
GTGCGTATCT ATGATGAGCA CTCAGAGCAA GTCAATTGCT GCCACTTCAC CAACAATAGT 
AATCACCTTC TCTTAGCCAC TGGTTCAAAT GACTACTTCC TTAAACTCTG GGATCTGAAT 
CAAGAAGAAT GTCGAAATAC CATGTTTGGC CATACAAATT CGGTCAGTCA CTGCAGATTT 
TCACCAGATG ATCAGGTTTT GGCCAGTTGT TCAGCTGATG GAACATTAAA GCTTTGGGAT 
GTGAAATCTG CAAATGAGAG GAAAAGCATT AATGTGAAGC AATTCTTCCT GAATTCAGAG 
GAGCCTCAGG AGGATCTGGA AGTGATAGTG AAATGCTGTT CGTGGTCTGC TGATGGTGCT 
AAGATAATGG TGGCTGCGAA AAATAAAGTC TTTCTTTTTG ACATTCACTC CAGTGGCCTG 
TTGGCAGAAA TGCACATGGG CCATCATAGC GCTGTCCAGT ACTGTGACTT CTCCCCCCAC 
AACCATTTGG CAGTGGTGGC TTTGTCCCAG TACTGTGTGG AGTTGTGGAG TATGGACTCA 
GGTTTAAAAG TAGCTGATTG CAGAGGACAT TTAAGTTGGG TCCATTGTGT GATGTTTTCT 
CCTGATGGAT CATCGTTTTT GACATCTTCT GATGACCAGA CAATCAGGCT CTGGGAGACA 
AAGAAAGTAT GTAAGAACTC TGCTACAGTG TTAAAGCAAG AAATAGATGT TGTGTTTCAA 
GAAAATGAAG TGACGGTTCT TGCAGTTGAC AATATAAGAC GTCTGCAACT CATTAATGGA 
AACACAGGTC AGATTGATTA CCTAACTGAA GCTCAAGTGA GTTGCTGTTG TTTAAGTCCA 
CATCTTCAGT ACCTTGCATT CGGAGGTGAA GATGGAGCCA TTCAGATTTT AGAACTCCTG 
AACAACAGAA TCTTCCAATC CAGAAATGGG CACAAGACCA CTGTTCGGCA CATCCAGTTC 
ACAGCTGATG GAAAGACTCT TATCTCAAGC TCTGATGATT CTGCAGTTCA GATATGGAAC 
TGGCAATCAG AGGACCCTTT CTTTCTCCAA GCCCACCAGG AAACAGTGAA AGACTTCAGA 
CTCTTGAAAA ATTCAAGACT TCTTTCCTGG TCATTTGATG GAACAGTAAA GGTATGGAAT 
ATTATTACCA GAAGAATAGA AAAAGATTTC GTGTGTCACC AGGATACAGT TCTTTCTTGT 
GATATTTCCT CTGATGCTAC TAAGTTTTCT TCTACCTCTG CTGACAAGAC TGCAAAGATT 
TGGAGTTTTG AACTCCTTTC TCCTCTTCAT GAATTGAGAG GCCACAAAGG CTGTGTGCGC 
TGCTCTTCCT TCTCTGTGAA CAATACCTTG TTGGCGACTG GAGACGACAA TGGAGAAATC 
AGGATATGGA ATGTCTCAAA CGGTGAGCTT CTTCATTTGT GTGCTCCGAT TTCAGTAGAA 
GAAGGAGCTG CTACTCATGG AGGCTGGGTG ACCGACCTTT GCTTTTCTCC AGACACCAAA 
ATGCTTGTTT CTGCTGGAGG ATATCTCAAG TGGTGGAATG TTGTCACTGG GGAATCCTCA 
CAGACCTTCT ACACAAACGG AACCAATCTT AAGAAAATAC ACGTGTCCCC TGACTTCAAA 
ACATATGTGA CTGTTGATAA TCTTGGTATT TTATATATTC TAAAGATTTT AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APAF1 ( XM_015471110.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326596.1 Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa19473
Clone ID Related Accession (Same CDS sequence)	XM_015471110.2 , XM_015471110.1
Accession Version	XM_015471110.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	apoptotic protease-activating factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_015471110.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCCTTGGA AAGGGACATC 
AAAACATCCT ACATTATGGA TCACATGATT AGTAATGGAG TTTTAACAGT GTCAGAGGAG 
GAAAAAGTGA AAAATGAGCC CACCCAACGG CAACGAGCAG CTATGCTAAT TAAAATGATA 
CTTAAAAAAG ATAATCATTC CTATATATCG TTCTACAATG CTCTACTTCA TGAAGGTTAC 
AAAGATCTTG CTGCCCTTCT GCATGGTGGC ATTCCTGTTA TCTCTTCTTC CGATGGCAAA 
GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
CAGAGGCCCG TTGTTTTTGT TACTAGGAAG AAGCTAGTGA ATGCAATTCA GCAGAAGCTC 
TCCAAACTGA ATGGTGAACC AGGATGGGTC ACCATATATG GGATGGCAGG TTGTGGGAAG 
TCTGTATTAG CTGCAGAAGC TGTTCGAGAT CATTCTTTCC TAGAAGATTG TTTCCCAGGA 
GGCGTGCATT GGGTGTCAGT TGGGAAACAG GACAAATCCG GGCTTCTGAT GAAACTACAG 
AACCTTTGTG CACGATTGGA TCAGGATGAG AGCTTCTCCC AGAGGCTTCC ACTTAATATT 
GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTGCGCA AACATCCAAG GTCTTTATTG 
ATCTTGGATG ATATTTGGGA TCCTTGGGTG TTAAAAGCTT TTGACAATCA GTGTCAAATT 
CTTCTCACAA CTAGAGATAA GAGTGTTACA GATTCAGTGA TGGGTCCTAA ATATGTAGTG 
CCTGTGGAGA GTGGTTTAGG AAAAGAAAAA GGACTTGAAA TTTTATCCCT TTTTGTTAAT 
ATGAAGAAAG CAGATTTGCC AGAACAAGCT CACAGTATTA TACAAGAATG TAAAGGTTCC 
CCTCTTGTAG TATCTTTAAT TGGTGCACTT TTACGAGATT TTCCCAACCG CTGGGAGTAC 
TACCTCAGGC AACTTCAGAA TAAGCAATTT AAGAGGATAA GAAAATCTTC ATCTTATGAC 
TATGAGGCTC TAGATGAAGC CATGTCTATA AGTGTTGAAA TGCTCAGAGA AGACATCAAA 
GATTATTACA CAGATCTTTC CATCTTACAG AAAGATGTTA AGGTGCCTAC AAAGGTGTTA 
TGTATCCTCT GGGACTTGGA AACGGAAGAA GTTGAAGACA TATTACAGGA ATTTGTTAAT 
AAGTCTCTCT TGTTCTGTGA CCGGAATGGA AAATCATTTC TTTATTATTT ACATGATCTG 
CAAGTAGATT TCCTTGCTGA AAAGAATCGC AGCCAGCTTC AGGATCTCCA CAAGAAGATG 
GTCACTCAGT TCCAGAGACA TCACCAGCTA TATTCTCTTT CACCAGATCA GGAGGATTGC 
ATGTATTGGT ACAGTTTTCT GGCCTATCAC ATGGCCAGTG CTAATATGCA CAAAGAACTG 
TGTGCTTTAA TGTTTTCCCT GGATTGGATT AAAGCAAAAA CGGAACTTGT AGGCCCTGCT 
CATCTGATTC ATGAATTTGT GGAATACAGG CATATATTGG ATGAAAAGGA TGGTATAGAC 
TGTGAGAATT TCCAGGAGTT TTTATCTTTA AATGGACATC TTCTTGGACG ACAGCCATTT 
CCTAATATTG TGCAACTGGG CCTCTGTGAA CTGGAAACTT CAGAGGTTTA TCGGCAAGCT 
AAGCTGCAGG CCAAGCAGGA GGTCGATAAC GGAATGCTTT ACCTGGAGTG GGTAAACAAA 
AAAAACATGA AGAACCTCTC CCGCTTGGTT GTCCGCCCCC ATACAGATGC TGTTTACCAT 
GCTTGCTTTT CTGAAGATGG TCAAAGAATA GCGTCTTGTG GAGCTGATAA AACTTTACAG 
GTATTCAAAG CTGAAACAGG AGAGAAACTT CTAGAAATCA AGGCTCATGA GGATGAAGTG 
CTTTGCTGTG CATTCTCTGC AGATGACAAA TTTATAGCAA CCTGCTCAGT GGATAAAAAA 
GTGAAGATTT GGAATTCAAT GACTGGGGAA CTAGTGCGTA TCTATGATGA GCACTCAGAG 
CAAGTCAATT GCTGCCACTT CACCAACAAT AGTAATCACC TTCTCTTAGC CACTGGTTCA 
AATGACTACT TCCTTAAACT CTGGGATCTG AATCAAGAAG AATGTCGAAA TACCATGTTT 
GGCCATACAA ATTCGGTCAG TCACTGCAGA TTTTCACCAG ATGATCAGGT TTTGGCCAGT 
TGTTCAGCTG ATGGAACATT AAAGCTTTGG GATGTGAAAT CTGCAAATGA GAGGAAAAGC 
ATTAATGTGA AGCAATTCTT CCTGAATTCA GAGGAGCCTC AGGAGGATCT GGAAGTGATA 
GTGAAATGCT GTTCGTGGTC TGCTGATGGT GCTAAGATAA TGGTGGCTGC GAAAAATAAA 
GTCTTTCTTT TTGACATTCA CTCCAGTGGC CTGTTGGCAG AAATGCACAT GGGCCATCAT 
AGCGCTGTCC AGTACTGTGA CTTCTCCCCC CACAACCATT TGGCAGTGGT GGCTTTGTCC 
CAGTACTGTG TGGAGTTGTG GAGTATGGAC TCAGGTTTAA AAGTAGCTGA TTGCAGAGGA 
CATTTAAGTT GGGTCCATTG TGTGATGTTT TCTCCTGATG GATCATCGTT TTTGACATCT 
TCTGATGACC AGACAATCAG GCTCTGGGAG ACAAAGAAAG TATGTAAGAA CTCTGCTACA 
GTGTTAAAGC AAGAAATAGA TGTTGTGTTT CAAGAAAATG AAGTGACGGT TCTTGCAGTT 
GACAATATAA GACGTCTGCA ACTCATTAAT GGAAACACAG GTCAGATTGA TTACCTAACT 
GAAGCTCAAG TGAGTTGCTG TTGTTTAAGT CCACATCTTC AGTACCTTGC ATTCGGAGGT 
GAAGATGGAG CCATTCAGAT TTTAGAACTC CTGAACAACA GAATCTTCCA ATCCAGAAAT 
GGGCACAAGA CCACTGTTCG GCACATCCAG TTCACAGCTG ATGGAAAGAC TCTTATCTCA 
AGCTCTGATG ATTCTGCAGT TCAGATATGG AACTGGCAAT CAGAGGACCC TTTCTTTCTC 
CAAGCCCACC AGGAAACAGT GAAAGACTTC AGACTCTTGA AAAATTCAAG ACTTCTTTCC 
TGGTCATTTG ATGGAACAGT AAAGGTATGG AATATTATTA CCAGAAGAAT AGAAAAAGAT 
TTCGTGTGTC ACCAGGATAC AGTTCTTTCT TGTGATATTT CCTCTGATGC TACTAAGTTT 
TCTTCTACCT CTGCTGACAA GACTGCAAAG ATTTGGAGTT TTGAACTCCT TTCTCCTCTT 
CATGAATTGA GAGGCCACAA AGGCTGTGTG CGCTGCTCTT CCTTCTCTGT GAACAATACC 
TTGTTGGCGA CTGGAGACGA CAATGGAGAA ATCAGGATAT GGAATGTCTC AAACGGTGAG 
CTTCTTCATT TGTGTGCTCC GATTTCAGTA GAAGAAGGAG CTGCTACTCA TGGAGGCTGG 
GTGACCGACC TTTGCTTTTC TCCAGACACC AAAATGCTTG TTTCTGCTGG AGGATATCTC 
AAGTGGTGGA ATGTTGTCAC TGGGGAATCC TCACAGACCT TCTACACAAA CGGAACCAAT 
CTTAAGAAAA TACACGTGTC CCCTGACTTC AAAACATATG TGACTGTTGA TAATCTTGGT 
ATTTTATATA TTCTAAAGAT TTTAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326596.1
CDS	497..4246
Translation	MDAKARNCLLQHREALERDIKTSYIMDHMISNGVLTVSEEEKVKNEPTQRQRAAMLIKMILKKDNHSYISFYNALLHEGYKDLAALLHGGIPVISSSDGKDSVSGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLNGEPGWVTIYGMAGCGKSVLAAEAVRDHSFLEDCFPGGVHWVSVGKQDKSGLLMKLQNLCARLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDIWDPWVLKAFDNQCQILLTTRDKSVTDSVMGPKYVVPVESGLGKEKGLEILSLFVNMKKADLPEQAHSIIQECKGSPLVVSLIGALLRDFPNRWEYYLRQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDLETEEVEDILQEFVNKSLLFCDRNGKSFLYYLHDLQVDFLAEKNRSQLQDLHKKMVTQFQRHHQLYSLSPDQEDCMYWYSFLAYHMASANMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDGIDCENFQEFLSLNGHLLGRQPFPNIVQLGLCELETSEVYRQAKLQAKQEVDNGMLYLEWVNKKNMKNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSADDKFIATCSVDKKVKIWNSMTGELVRIYDEHSEQVNCCHFTNNSNHLLLATGSNDYFLKLWDLNQEECRNTMFGHTNSVSHCRFSPDDQVLASCSADGTLKLWDVKSANERKSINVKQFFLNSEEPQEDLEVIVKCCSWSADGAKIMVAAKNKVFLFDIHSSGLLAEMHMGHHSAVQYCDFSPHNHLAVVALSQYCVELWSMDSGLKVADCRGHLSWVHCVMFSPDGSSFLTSSDDQTIRLWETKKVCKNSATVLKQEIDVVFQENEVTVLAVDNIRRLQLINGNTGQIDYLTEAQVSCCCLSPHLQYLAFGGEDGAIQILELLNNRIFQSRNGHKTTVRHIQFTADGKTLISSSDDSAVQIWNWQSEDPFFLQAHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITRRIEKDFVCHQDTVLSCDISSDATKFSSTSADKTAKIWSFELLSPLHELRGHKGCVRCSSFSVNNTLLATGDDNGEIRIWNVSNGELLHLCAPISVEEGAATHGGWVTDLCFSPDTKMLVSAGGYLKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILKILE

Target ORF information:

RefSeq Version	XM_015471110.2
Organism	Bos taurus(cattle)
Definition	Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471110.2

ORF Insert Sequence:

ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCCTTGGA AAGGGACATC 
AAAACATCCT ACATTATGGA TCACATGATT AGTAATGGAG TTTTAACAGT GTCAGAGGAG 
GAAAAAGTGA AAAATGAGCC CACCCAACGG CAACGAGCAG CTATGCTAAT TAAAATGATA 
CTTAAAAAAG ATAATCATTC CTATATATCG TTCTACAATG CTCTACTTCA TGAAGGTTAC 
AAAGATCTTG CTGCCCTTCT GCATGGTGGC ATTCCTGTTA TCTCTTCTTC CGATGGCAAA 
GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
CAGAGGCCCG TTGTTTTTGT TACTAGGAAG AAGCTAGTGA ATGCAATTCA GCAGAAGCTC 
TCCAAACTGA ATGGTGAACC AGGATGGGTC ACCATATATG GGATGGCAGG TTGTGGGAAG 
TCTGTATTAG CTGCAGAAGC TGTTCGAGAT CATTCTTTCC TAGAAGATTG TTTCCCAGGA 
GGCGTGCATT GGGTGTCAGT TGGGAAACAG GACAAATCCG GGCTTCTGAT GAAACTACAG 
AACCTTTGTG CACGATTGGA TCAGGATGAG AGCTTCTCCC AGAGGCTTCC ACTTAATATT 
GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTGCGCA AACATCCAAG GTCTTTATTG 
ATCTTGGATG ATATTTGGGA TCCTTGGGTG TTAAAAGCTT TTGACAATCA GTGTCAAATT 
CTTCTCACAA CTAGAGATAA GAGTGTTACA GATTCAGTGA TGGGTCCTAA ATATGTAGTG 
CCTGTGGAGA GTGGTTTAGG AAAAGAAAAA GGACTTGAAA TTTTATCCCT TTTTGTTAAT 
ATGAAGAAAG CAGATTTGCC AGAACAAGCT CACAGTATTA TACAAGAATG TAAAGGTTCC 
CCTCTTGTAG TATCTTTAAT TGGTGCACTT TTACGAGATT TTCCCAACCG CTGGGAGTAC 
TACCTCAGGC AACTTCAGAA TAAGCAATTT AAGAGGATAA GAAAATCTTC ATCTTATGAC 
TATGAGGCTC TAGATGAAGC CATGTCTATA AGTGTTGAAA TGCTCAGAGA AGACATCAAA 
GATTATTACA CAGATCTTTC CATCTTACAG AAAGATGTTA AGGTGCCTAC AAAGGTGTTA 
TGTATCCTCT GGGACTTGGA AACGGAAGAA GTTGAAGACA TATTACAGGA ATTTGTTAAT 
AAGTCTCTCT TGTTCTGTGA CCGGAATGGA AAATCATTTC TTTATTATTT ACATGATCTG 
CAAGTAGATT TCCTTGCTGA AAAGAATCGC AGCCAGCTTC AGGATCTCCA CAAGAAGATG 
GTCACTCAGT TCCAGAGACA TCACCAGCTA TATTCTCTTT CACCAGATCA GGAGGATTGC 
ATGTATTGGT ACAGTTTTCT GGCCTATCAC ATGGCCAGTG CTAATATGCA CAAAGAACTG 
TGTGCTTTAA TGTTTTCCCT GGATTGGATT AAAGCAAAAA CGGAACTTGT AGGCCCTGCT 
CATCTGATTC ATGAATTTGT GGAATACAGG CATATATTGG ATGAAAAGGA TGGTATAGAC 
TGTGAGAATT TCCAGGAGTT TTTATCTTTA AATGGACATC TTCTTGGACG ACAGCCATTT 
CCTAATATTG TGCAACTGGG CCTCTGTGAA CTGGAAACTT CAGAGGTTTA TCGGCAAGCT 
AAGCTGCAGG CCAAGCAGGA GGTCGATAAC GGAATGCTTT ACCTGGAGTG GGTAAACAAA 
AAAAACATGA AGAACCTCTC CCGCTTGGTT GTCCGCCCCC ATACAGATGC TGTTTACCAT 
GCTTGCTTTT CTGAAGATGG TCAAAGAATA GCGTCTTGTG GAGCTGATAA AACTTTACAG 
GTATTCAAAG CTGAAACAGG AGAGAAACTT CTAGAAATCA AGGCTCATGA GGATGAAGTG 
CTTTGCTGTG CATTCTCTGC AGATGACAAA TTTATAGCAA CCTGCTCAGT GGATAAAAAA 
GTGAAGATTT GGAATTCAAT GACTGGGGAA CTAGTGCGTA TCTATGATGA GCACTCAGAG 
CAAGTCAATT GCTGCCACTT CACCAACAAT AGTAATCACC TTCTCTTAGC CACTGGTTCA 
AATGACTACT TCCTTAAACT CTGGGATCTG AATCAAGAAG AATGTCGAAA TACCATGTTT 
GGCCATACAA ATTCGGTCAG TCACTGCAGA TTTTCACCAG ATGATCAGGT TTTGGCCAGT 
TGTTCAGCTG ATGGAACATT AAAGCTTTGG GATGTGAAAT CTGCAAATGA GAGGAAAAGC 
ATTAATGTGA AGCAATTCTT CCTGAATTCA GAGGAGCCTC AGGAGGATCT GGAAGTGATA 
GTGAAATGCT GTTCGTGGTC TGCTGATGGT GCTAAGATAA TGGTGGCTGC GAAAAATAAA 
GTCTTTCTTT TTGACATTCA CTCCAGTGGC CTGTTGGCAG AAATGCACAT GGGCCATCAT 
AGCGCTGTCC AGTACTGTGA CTTCTCCCCC CACAACCATT TGGCAGTGGT GGCTTTGTCC 
CAGTACTGTG TGGAGTTGTG GAGTATGGAC TCAGGTTTAA AAGTAGCTGA TTGCAGAGGA 
CATTTAAGTT GGGTCCATTG TGTGATGTTT TCTCCTGATG GATCATCGTT TTTGACATCT 
TCTGATGACC AGACAATCAG GCTCTGGGAG ACAAAGAAAG TATGTAAGAA CTCTGCTACA 
GTGTTAAAGC AAGAAATAGA TGTTGTGTTT CAAGAAAATG AAGTGACGGT TCTTGCAGTT 
GACAATATAA GACGTCTGCA ACTCATTAAT GGAAACACAG GTCAGATTGA TTACCTAACT 
GAAGCTCAAG TGAGTTGCTG TTGTTTAAGT CCACATCTTC AGTACCTTGC ATTCGGAGGT 
GAAGATGGAG CCATTCAGAT TTTAGAACTC CTGAACAACA GAATCTTCCA ATCCAGAAAT 
GGGCACAAGA CCACTGTTCG GCACATCCAG TTCACAGCTG ATGGAAAGAC TCTTATCTCA 
AGCTCTGATG ATTCTGCAGT TCAGATATGG AACTGGCAAT CAGAGGACCC TTTCTTTCTC 
CAAGCCCACC AGGAAACAGT GAAAGACTTC AGACTCTTGA AAAATTCAAG ACTTCTTTCC 
TGGTCATTTG ATGGAACAGT AAAGGTATGG AATATTATTA CCAGAAGAAT AGAAAAAGAT 
TTCGTGTGTC ACCAGGATAC AGTTCTTTCT TGTGATATTT CCTCTGATGC TACTAAGTTT 
TCTTCTACCT CTGCTGACAA GACTGCAAAG ATTTGGAGTT TTGAACTCCT TTCTCCTCTT 
CATGAATTGA GAGGCCACAA AGGCTGTGTG CGCTGCTCTT CCTTCTCTGT GAACAATACC 
TTGTTGGCGA CTGGAGACGA CAATGGAGAA ATCAGGATAT GGAATGTCTC AAACGGTGAG 
CTTCTTCATT TGTGTGCTCC GATTTCAGTA GAAGAAGGAG CTGCTACTCA TGGAGGCTGG 
GTGACCGACC TTTGCTTTTC TCCAGACACC AAAATGCTTG TTTCTGCTGG AGGATATCTC 
AAGTGGTGGA ATGTTGTCAC TGGGGAATCC TCACAGACCT TCTACACAAA CGGAACCAAT 
CTTAAGAAAA TACACGTGTC CCCTGACTTC AAAACATATG TGACTGTTGA TAATCTTGGT 
ATTTTATATA TTCTAAAGAT TTTAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APAF1 ( XM_015471110.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326596.1 Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X1, mRNA.

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CloneID	OBa19473
Clone ID Related Accession (Same CDS sequence)	XM_015471110.2 , XM_015471110.1
Accession Version	XM_015471110.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	apoptotic protease-activating factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCCTTGGA AAGGGACATC 
AAAACATCCT ACATTATGGA TCACATGATT AGTAATGGAG TTTTAACAGT GTCAGAGGAG 
GAAAAAGTGA AAAATGAGCC CACCCAACGG CAACGAGCAG CTATGCTAAT TAAAATGATA 
CTTAAAAAAG ATAATCATTC CTATATATCG TTCTACAATG CTCTACTTCA TGAAGGTTAC 
AAAGATCTTG CTGCCCTTCT GCATGGTGGC ATTCCTGTTA TCTCTTCTTC CGATGGCAAA 
GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
CAGAGGCCCG TTGTTTTTGT TACTAGGAAG AAGCTAGTGA ATGCAATTCA GCAGAAGCTC 
TCCAAACTGA ATGGTGAACC AGGATGGGTC ACCATATATG GGATGGCAGG TTGTGGGAAG 
TCTGTATTAG CTGCAGAAGC TGTTCGAGAT CATTCTTTCC TAGAAGATTG TTTCCCAGGA 
GGCGTGCATT GGGTGTCAGT TGGGAAACAG GACAAATCCG GGCTTCTGAT GAAACTACAG 
AACCTTTGTG CACGATTGGA TCAGGATGAG AGCTTCTCCC AGAGGCTTCC ACTTAATATT 
GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTGCGCA AACATCCAAG GTCTTTATTG 
ATCTTGGATG ATATTTGGGA TCCTTGGGTG TTAAAAGCTT TTGACAATCA GTGTCAAATT 
CTTCTCACAA CTAGAGATAA GAGTGTTACA GATTCAGTGA TGGGTCCTAA ATATGTAGTG 
CCTGTGGAGA GTGGTTTAGG AAAAGAAAAA GGACTTGAAA TTTTATCCCT TTTTGTTAAT 
ATGAAGAAAG CAGATTTGCC AGAACAAGCT CACAGTATTA TACAAGAATG TAAAGGTTCC 
CCTCTTGTAG TATCTTTAAT TGGTGCACTT TTACGAGATT TTCCCAACCG CTGGGAGTAC 
TACCTCAGGC AACTTCAGAA TAAGCAATTT AAGAGGATAA GAAAATCTTC ATCTTATGAC 
TATGAGGCTC TAGATGAAGC CATGTCTATA AGTGTTGAAA TGCTCAGAGA AGACATCAAA 
GATTATTACA CAGATCTTTC CATCTTACAG AAAGATGTTA AGGTGCCTAC AAAGGTGTTA 
TGTATCCTCT GGGACTTGGA AACGGAAGAA GTTGAAGACA TATTACAGGA ATTTGTTAAT 
AAGTCTCTCT TGTTCTGTGA CCGGAATGGA AAATCATTTC TTTATTATTT ACATGATCTG 
CAAGTAGATT TCCTTGCTGA AAAGAATCGC AGCCAGCTTC AGGATCTCCA CAAGAAGATG 
GTCACTCAGT TCCAGAGACA TCACCAGCTA TATTCTCTTT CACCAGATCA GGAGGATTGC 
ATGTATTGGT ACAGTTTTCT GGCCTATCAC ATGGCCAGTG CTAATATGCA CAAAGAACTG 
TGTGCTTTAA TGTTTTCCCT GGATTGGATT AAAGCAAAAA CGGAACTTGT AGGCCCTGCT 
CATCTGATTC ATGAATTTGT GGAATACAGG CATATATTGG ATGAAAAGGA TGGTATAGAC 
TGTGAGAATT TCCAGGAGTT TTTATCTTTA AATGGACATC TTCTTGGACG ACAGCCATTT 
CCTAATATTG TGCAACTGGG CCTCTGTGAA CTGGAAACTT CAGAGGTTTA TCGGCAAGCT 
AAGCTGCAGG CCAAGCAGGA GGTCGATAAC GGAATGCTTT ACCTGGAGTG GGTAAACAAA 
AAAAACATGA AGAACCTCTC CCGCTTGGTT GTCCGCCCCC ATACAGATGC TGTTTACCAT 
GCTTGCTTTT CTGAAGATGG TCAAAGAATA GCGTCTTGTG GAGCTGATAA AACTTTACAG 
GTATTCAAAG CTGAAACAGG AGAGAAACTT CTAGAAATCA AGGCTCATGA GGATGAAGTG 
CTTTGCTGTG CATTCTCTGC AGATGACAAA TTTATAGCAA CCTGCTCAGT GGATAAAAAA 
GTGAAGATTT GGAATTCAAT GACTGGGGAA CTAGTGCGTA TCTATGATGA GCACTCAGAG 
CAAGTCAATT GCTGCCACTT CACCAACAAT AGTAATCACC TTCTCTTAGC CACTGGTTCA 
AATGACTACT TCCTTAAACT CTGGGATCTG AATCAAGAAG AATGTCGAAA TACCATGTTT 
GGCCATACAA ATTCGGTCAG TCACTGCAGA TTTTCACCAG ATGATCAGGT TTTGGCCAGT 
TGTTCAGCTG ATGGAACATT AAAGCTTTGG GATGTGAAAT CTGCAAATGA GAGGAAAAGC 
ATTAATGTGA AGCAATTCTT CCTGAATTCA GAGGAGCCTC AGGAGGATCT GGAAGTGATA 
GTGAAATGCT GTTCGTGGTC TGCTGATGGT GCTAAGATAA TGGTGGCTGC GAAAAATAAA 
GTCTTTCTTT TTGACATTCA CTCCAGTGGC CTGTTGGCAG AAATGCACAT GGGCCATCAT 
AGCGCTGTCC AGTACTGTGA CTTCTCCCCC CACAACCATT TGGCAGTGGT GGCTTTGTCC 
CAGTACTGTG TGGAGTTGTG GAGTATGGAC TCAGGTTTAA AAGTAGCTGA TTGCAGAGGA 
CATTTAAGTT GGGTCCATTG TGTGATGTTT TCTCCTGATG GATCATCGTT TTTGACATCT 
TCTGATGACC AGACAATCAG GCTCTGGGAG ACAAAGAAAG TATGTAAGAA CTCTGCTACA 
GTGTTAAAGC AAGAAATAGA TGTTGTGTTT CAAGAAAATG AAGTGACGGT TCTTGCAGTT 
GACAATATAA GACGTCTGCA ACTCATTAAT GGAAACACAG GTCAGATTGA TTACCTAACT 
GAAGCTCAAG TGAGTTGCTG TTGTTTAAGT CCACATCTTC AGTACCTTGC ATTCGGAGGT 
GAAGATGGAG CCATTCAGAT TTTAGAACTC CTGAACAACA GAATCTTCCA ATCCAGAAAT 
GGGCACAAGA CCACTGTTCG GCACATCCAG TTCACAGCTG ATGGAAAGAC TCTTATCTCA 
AGCTCTGATG ATTCTGCAGT TCAGATATGG AACTGGCAAT CAGAGGACCC TTTCTTTCTC 
CAAGCCCACC AGGAAACAGT GAAAGACTTC AGACTCTTGA AAAATTCAAG ACTTCTTTCC 
TGGTCATTTG ATGGAACAGT AAAGGTATGG AATATTATTA CCAGAAGAAT AGAAAAAGAT 
TTCGTGTGTC ACCAGGATAC AGTTCTTTCT TGTGATATTT CCTCTGATGC TACTAAGTTT 
TCTTCTACCT CTGCTGACAA GACTGCAAAG ATTTGGAGTT TTGAACTCCT TTCTCCTCTT 
CATGAATTGA GAGGCCACAA AGGCTGTGTG CGCTGCTCTT CCTTCTCTGT GAACAATACC 
TTGTTGGCGA CTGGAGACGA CAATGGAGAA ATCAGGATAT GGAATGTCTC AAACGGTGAG 
CTTCTTCATT TGTGTGCTCC GATTTCAGTA GAAGAAGGAG CTGCTACTCA TGGAGGCTGG 
GTGACCGACC TTTGCTTTTC TCCAGACACC AAAATGCTTG TTTCTGCTGG AGGATATCTC 
AAGTGGTGGA ATGTTGTCAC TGGGGAATCC TCACAGACCT TCTACACAAA CGGAACCAAT 
CTTAAGAAAA TACACGTGTC CCCTGACTTC AAAACATATG TGACTGTTGA TAATCTTGGT 
ATTTTATATA TTCTAAAGAT TTTAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326596.1
CDS	469..4218
Translation	MDAKARNCLLQHREALERDIKTSYIMDHMISNGVLTVSEEEKVKNEPTQRQRAAMLIKMILKKDNHSYISFYNALLHEGYKDLAALLHGGIPVISSSDGKDSVSGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLNGEPGWVTIYGMAGCGKSVLAAEAVRDHSFLEDCFPGGVHWVSVGKQDKSGLLMKLQNLCARLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDIWDPWVLKAFDNQCQILLTTRDKSVTDSVMGPKYVVPVESGLGKEKGLEILSLFVNMKKADLPEQAHSIIQECKGSPLVVSLIGALLRDFPNRWEYYLRQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDLETEEVEDILQEFVNKSLLFCDRNGKSFLYYLHDLQVDFLAEKNRSQLQDLHKKMVTQFQRHHQLYSLSPDQEDCMYWYSFLAYHMASANMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDGIDCENFQEFLSLNGHLLGRQPFPNIVQLGLCELETSEVYRQAKLQAKQEVDNGMLYLEWVNKKNMKNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSADDKFIATCSVDKKVKIWNSMTGELVRIYDEHSEQVNCCHFTNNSNHLLLATGSNDYFLKLWDLNQEECRNTMFGHTNSVSHCRFSPDDQVLASCSADGTLKLWDVKSANERKSINVKQFFLNSEEPQEDLEVIVKCCSWSADGAKIMVAAKNKVFLFDIHSSGLLAEMHMGHHSAVQYCDFSPHNHLAVVALSQYCVELWSMDSGLKVADCRGHLSWVHCVMFSPDGSSFLTSSDDQTIRLWETKKVCKNSATVLKQEIDVVFQENEVTVLAVDNIRRLQLINGNTGQIDYLTEAQVSCCCLSPHLQYLAFGGEDGAIQILELLNNRIFQSRNGHKTTVRHIQFTADGKTLISSSDDSAVQIWNWQSEDPFFLQAHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITRRIEKDFVCHQDTVLSCDISSDATKFSSTSADKTAKIWSFELLSPLHELRGHKGCVRCSSFSVNNTLLATGDDNGEIRIWNVSNGELLHLCAPISVEEGAATHGGWVTDLCFSPDTKMLVSAGGYLKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILKILE

Target ORF information:

RefSeq Version	XM_015471110.1
Organism	Bos taurus(cattle)
Definition	Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015471110.1

ORF Insert Sequence:

ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCCTTGGA AAGGGACATC 
AAAACATCCT ACATTATGGA TCACATGATT AGTAATGGAG TTTTAACAGT GTCAGAGGAG 
GAAAAAGTGA AAAATGAGCC CACCCAACGG CAACGAGCAG CTATGCTAAT TAAAATGATA 
CTTAAAAAAG ATAATCATTC CTATATATCG TTCTACAATG CTCTACTTCA TGAAGGTTAC 
AAAGATCTTG CTGCCCTTCT GCATGGTGGC ATTCCTGTTA TCTCTTCTTC CGATGGCAAA 
GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
CAGAGGCCCG TTGTTTTTGT TACTAGGAAG AAGCTAGTGA ATGCAATTCA GCAGAAGCTC 
TCCAAACTGA ATGGTGAACC AGGATGGGTC ACCATATATG GGATGGCAGG TTGTGGGAAG 
TCTGTATTAG CTGCAGAAGC TGTTCGAGAT CATTCTTTCC TAGAAGATTG TTTCCCAGGA 
GGCGTGCATT GGGTGTCAGT TGGGAAACAG GACAAATCCG GGCTTCTGAT GAAACTACAG 
AACCTTTGTG CACGATTGGA TCAGGATGAG AGCTTCTCCC AGAGGCTTCC ACTTAATATT 
GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTGCGCA AACATCCAAG GTCTTTATTG 
ATCTTGGATG ATATTTGGGA TCCTTGGGTG TTAAAAGCTT TTGACAATCA GTGTCAAATT 
CTTCTCACAA CTAGAGATAA GAGTGTTACA GATTCAGTGA TGGGTCCTAA ATATGTAGTG 
CCTGTGGAGA GTGGTTTAGG AAAAGAAAAA GGACTTGAAA TTTTATCCCT TTTTGTTAAT 
ATGAAGAAAG CAGATTTGCC AGAACAAGCT CACAGTATTA TACAAGAATG TAAAGGTTCC 
CCTCTTGTAG TATCTTTAAT TGGTGCACTT TTACGAGATT TTCCCAACCG CTGGGAGTAC 
TACCTCAGGC AACTTCAGAA TAAGCAATTT AAGAGGATAA GAAAATCTTC ATCTTATGAC 
TATGAGGCTC TAGATGAAGC CATGTCTATA AGTGTTGAAA TGCTCAGAGA AGACATCAAA 
GATTATTACA CAGATCTTTC CATCTTACAG AAAGATGTTA AGGTGCCTAC AAAGGTGTTA 
TGTATCCTCT GGGACTTGGA AACGGAAGAA GTTGAAGACA TATTACAGGA ATTTGTTAAT 
AAGTCTCTCT TGTTCTGTGA CCGGAATGGA AAATCATTTC TTTATTATTT ACATGATCTG 
CAAGTAGATT TCCTTGCTGA AAAGAATCGC AGCCAGCTTC AGGATCTCCA CAAGAAGATG 
GTCACTCAGT TCCAGAGACA TCACCAGCTA TATTCTCTTT CACCAGATCA GGAGGATTGC 
ATGTATTGGT ACAGTTTTCT GGCCTATCAC ATGGCCAGTG CTAATATGCA CAAAGAACTG 
TGTGCTTTAA TGTTTTCCCT GGATTGGATT AAAGCAAAAA CGGAACTTGT AGGCCCTGCT 
CATCTGATTC ATGAATTTGT GGAATACAGG CATATATTGG ATGAAAAGGA TGGTATAGAC 
TGTGAGAATT TCCAGGAGTT TTTATCTTTA AATGGACATC TTCTTGGACG ACAGCCATTT 
CCTAATATTG TGCAACTGGG CCTCTGTGAA CTGGAAACTT CAGAGGTTTA TCGGCAAGCT 
AAGCTGCAGG CCAAGCAGGA GGTCGATAAC GGAATGCTTT ACCTGGAGTG GGTAAACAAA 
AAAAACATGA AGAACCTCTC CCGCTTGGTT GTCCGCCCCC ATACAGATGC TGTTTACCAT 
GCTTGCTTTT CTGAAGATGG TCAAAGAATA GCGTCTTGTG GAGCTGATAA AACTTTACAG 
GTATTCAAAG CTGAAACAGG AGAGAAACTT CTAGAAATCA AGGCTCATGA GGATGAAGTG 
CTTTGCTGTG CATTCTCTGC AGATGACAAA TTTATAGCAA CCTGCTCAGT GGATAAAAAA 
GTGAAGATTT GGAATTCAAT GACTGGGGAA CTAGTGCGTA TCTATGATGA GCACTCAGAG 
CAAGTCAATT GCTGCCACTT CACCAACAAT AGTAATCACC TTCTCTTAGC CACTGGTTCA 
AATGACTACT TCCTTAAACT CTGGGATCTG AATCAAGAAG AATGTCGAAA TACCATGTTT 
GGCCATACAA ATTCGGTCAG TCACTGCAGA TTTTCACCAG ATGATCAGGT TTTGGCCAGT 
TGTTCAGCTG ATGGAACATT AAAGCTTTGG GATGTGAAAT CTGCAAATGA GAGGAAAAGC 
ATTAATGTGA AGCAATTCTT CCTGAATTCA GAGGAGCCTC AGGAGGATCT GGAAGTGATA 
GTGAAATGCT GTTCGTGGTC TGCTGATGGT GCTAAGATAA TGGTGGCTGC GAAAAATAAA 
GTCTTTCTTT TTGACATTCA CTCCAGTGGC CTGTTGGCAG AAATGCACAT GGGCCATCAT 
AGCGCTGTCC AGTACTGTGA CTTCTCCCCC CACAACCATT TGGCAGTGGT GGCTTTGTCC 
CAGTACTGTG TGGAGTTGTG GAGTATGGAC TCAGGTTTAA AAGTAGCTGA TTGCAGAGGA 
CATTTAAGTT GGGTCCATTG TGTGATGTTT TCTCCTGATG GATCATCGTT TTTGACATCT 
TCTGATGACC AGACAATCAG GCTCTGGGAG ACAAAGAAAG TATGTAAGAA CTCTGCTACA 
GTGTTAAAGC AAGAAATAGA TGTTGTGTTT CAAGAAAATG AAGTGACGGT TCTTGCAGTT 
GACAATATAA GACGTCTGCA ACTCATTAAT GGAAACACAG GTCAGATTGA TTACCTAACT 
GAAGCTCAAG TGAGTTGCTG TTGTTTAAGT CCACATCTTC AGTACCTTGC ATTCGGAGGT 
GAAGATGGAG CCATTCAGAT TTTAGAACTC CTGAACAACA GAATCTTCCA ATCCAGAAAT 
GGGCACAAGA CCACTGTTCG GCACATCCAG TTCACAGCTG ATGGAAAGAC TCTTATCTCA 
AGCTCTGATG ATTCTGCAGT TCAGATATGG AACTGGCAAT CAGAGGACCC TTTCTTTCTC 
CAAGCCCACC AGGAAACAGT GAAAGACTTC AGACTCTTGA AAAATTCAAG ACTTCTTTCC 
TGGTCATTTG ATGGAACAGT AAAGGTATGG AATATTATTA CCAGAAGAAT AGAAAAAGAT 
TTCGTGTGTC ACCAGGATAC AGTTCTTTCT TGTGATATTT CCTCTGATGC TACTAAGTTT 
TCTTCTACCT CTGCTGACAA GACTGCAAAG ATTTGGAGTT TTGAACTCCT TTCTCCTCTT 
CATGAATTGA GAGGCCACAA AGGCTGTGTG CGCTGCTCTT CCTTCTCTGT GAACAATACC 
TTGTTGGCGA CTGGAGACGA CAATGGAGAA ATCAGGATAT GGAATGTCTC AAACGGTGAG 
CTTCTTCATT TGTGTGCTCC GATTTCAGTA GAAGAAGGAG CTGCTACTCA TGGAGGCTGG 
GTGACCGACC TTTGCTTTTC TCCAGACACC AAAATGCTTG TTTCTGCTGG AGGATATCTC 
AAGTGGTGGA ATGTTGTCAC TGGGGAATCC TCACAGACCT TCTACACAAA CGGAACCAAT 
CTTAAGAAAA TACACGTGTC CCCTGACTTC AAAACATATG TGACTGTTGA TAATCTTGGT 
ATTTTATATA TTCTAAAGAT TTTAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APAF1 ( XM_010805196.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803498.1 Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa53779
Clone ID Related Accession (Same CDS sequence)	XM_010805196.2 , XM_010805196.3
Accession Version	XM_010805196.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	apoptotic protease-activating factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805196.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCCTTGGA AAGGGACATC 
AAAACATCCT ACATTATGGA TCACATGATT AGTAATGGAG TTTTAACAGT GTCAGAGGAG 
GAAAAAGTGA AAAATGAGCC CACCCAACGG CAACGAGCAG CTATGCTAAT TAAAATGATA 
CTTAAAAAAG ATAATCATTC CTATATATCG TTCTACAATG CTCTACTTCA TGAAGGTTAC 
AAAGATCTTG CTGCCCTTCT GCATGGTGGC ATTCCTGTTA TCTCTTCTTC CGATGGCAAA 
GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
CAGAGGCCCG TTGTTTTTGT TACTAGGAAG AAGCTAGTGA ATGCAATTCA GCAGAAGCTC 
TCCAAACTGA ATGGTGAACC AGGATGGGTC ACCATATATG GGATGGCAGG TTGTGGGAAG 
TCTGTATTAG CTGCAGAAGC TGTTCGAGAT CATTCTTTCC TAGAAGATTG TTTCCCAGGA 
GGCGTGCATT GGGTGTCAGT TGGGAAACAG GACAAATCCG GGCTTCTGAT GAAACTACAG 
AACCTTTGTG CACGATTGGA TCAGGATGAG AGCTTCTCCC AGAGGCTTCC ACTTAATATT 
GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTGCGCA AACATCCAAG GTCTTTATTG 
ATCTTGGATG ATATTTGGGA TCCTTGGGTG TTAAAAGCTT TTGACAATCA GTGTCAAATT 
CTTCTCACAA CTAGAGATAA GAGTGTTACA GATTCAGTGA TGGGTTCCCC TCTTGTAGTA 
TCTTTAATTG GTGCACTTTT ACGAGATTTT CCCAACCGCT GGGAGTACTA CCTCAGGCAA 
CTTCAGAATA AGCAATTTAA GAGGATAAGA AAATCTTCAT CTTATGACTA TGAGGCTCTA 
GATGAAGCCA TGTCTATAAG TGTTGAAATG CTCAGAGAAG ACATCAAAGA TTATTACACA 
GATCTTTCCA TCTTACAGAA AGATGTTAAG GTGCCTACAA AGGTGTTATG TATCCTCTGG 
GACTTGGAAA CGGAAGAAGT TGAAGACATA TTACAGGAAT TTGTTAATAA GTCTCTCTTG 
TTCTGTGACC GGAATGGAAA ATCATTTCTT TATTATTTAC ATGATCTGCA AGTAGATTTC 
CTTGCTGAAA AGAATCGCAG CCAGCTTCAG GATCTCCACA AGAAGATGGT CACTCAGTTC 
CAGAGACATC ACCAGCTATA TTCTCTTTCA CCAGATCAGG AGGATTGCAT GTATTGGTAC 
AGTTTTCTGG CCTATCACAT GGCCAGTGCT AATATGCACA AAGAACTGTG TGCTTTAATG 
TTTTCCCTGG ATTGGATTAA AGCAAAAACG GAACTTGTAG GCCCTGCTCA TCTGATTCAT 
GAATTTGTGG AATACAGGCA TATATTGGAT GAAAAGGATG GTATAGACTG TGAGAATTTC 
CAGGAGTTTT TATCTTTAAA TGGACATCTT CTTGGACGAC AGCCATTTCC TAATATTGTG 
CAACTGGGCC TCTGTGAACT GGAAACTTCA GAGGTTTATC GGCAAGCTAA GCTGCAGGCC 
AAGCAGGAGG TCGATAACGG AATGCTTTAC CTGGAGTGGG TAAACAAAAA AAACATGAAG 
AACCTCTCCC GCTTGGTTGT CCGCCCCCAT ACAGATGCTG TTTACCATGC TTGCTTTTCT 
GAAGATGGTC AAAGAATAGC GTCTTGTGGA GCTGATAAAA CTTTACAGGT ATTCAAAGCT 
GAAACAGGAG AGAAACTTCT AGAAATCAAG GCTCATGAGG ATGAAGTGCT TTGCTGTGCA 
TTCTCTGCAG ATGACAAATT TATAGCAACC TGCTCAGTGG ATAAAAAAGT GAAGATTTGG 
AATTCAATGA CTGGGGAACT AGTGCGTATC TATGATGAGC ACTCAGAGCA AGTCAATTGC 
TGCCACTTCA CCAACAATAG TAATCACCTT CTCTTAGCCA CTGGTTCAAA TGACTACTTC 
CTTAAACTCT GGGATCTGAA TCAAGAAGAA TGTCGAAATA CCATGTTTGG CCATACAAAT 
TCGGTCAGTC ACTGCAGATT TTCACCAGAT GATCAGGTTT TGGCCAGTTG TTCAGCTGAT 
GGAACATTAA AGCTTTGGGA TGTGAAATCT GCAAATGAGA GGAAAAGCAT TAATGTGAAG 
CAATTCTTCC TGAATTCAGA GGAGCCTCAG GAGGATCTGG AAGTGATAGT GAAATGCTGT 
TCGTGGTCTG CTGATGGTGC TAAGATAATG GTGGCTGCGA AAAATAAAGT CTTTCTTTTT 
GACATTCACT CCAGTGGCCT GTTGGCAGAA ATGCACATGG GCCATCATAG CGCTGTCCAG 
TACTGTGACT TCTCCCCCCA CAACCATTTG GCAGTGGTGG CTTTGTCCCA GTACTGTGTG 
GAGTTGTGGA GTATGGACTC AGGTTTAAAA GTAGCTGATT GCAGAGGACA TTTAAGTTGG 
GTCCATTGTG TGATGTTTTC TCCTGATGGA TCATCGTTTT TGACATCTTC TGATGACCAG 
ACAATCAGGC TCTGGGAGAC AAAGAAAGTA TGTAAGAACT CTGCTACAGT GTTAAAGCAA 
GAAATAGATG TTGTGTTTCA AGAAAATGAA GTGACGGTTC TTGCAGTTGA CAATATAAGA 
CGTCTGCAAC TCATTAATGG AAACACAGGT CAGATTGATT ACCTAACTGA AGCTCAAGTG 
AGTTGCTGTT GTTTAAGTCC ACATCTTCAG TACCTTGCAT TCGGAGGTGA AGATGGAGCC 
ATTCAGATTT TAGAACTCCT GAACAACAGA ATCTTCCAAT CCAGAAATGG GCACAAGACC 
ACTGTTCGGC ACATCCAGTT CACAGCTGAT GGAAAGACTC TTATCTCAAG CTCTGATGAT 
TCTGCAGTTC AGATATGGAA CTGGCAATCA GAGGACCCTT TCTTTCTCCA AGCCCACCAG 
GAAACAGTGA AAGACTTCAG ACTCTTGAAA AATTCAAGAC TTCTTTCCTG GTCATTTGAT 
GGAACAGTAA AGGTATGGAA TATTATTACC AGAAGAATAG AAAAAGATTT CGTGTGTCAC 
CAGGATACAG TTCTTTCTTG TGATATTTCC TCTGATGCTA CTAAGTTTTC TTCTACCTCT 
GCTGACAAGA CTGCAAAGAT TTGGAGTTTT GAACTCCTTT CTCCTCTTCA TGAATTGAGA 
GGCCACAAAG GCTGTGTGCG CTGCTCTTCC TTCTCTGTGA ACAATACCTT GTTGGCGACT 
GGAGACGACA ATGGAGAAAT CAGGATATGG AATGTCTCAA ACGGTGAGCT TCTTCATTTG 
TGTGCTCCGA TTTCAGTAGA AGAAGGAGCT GCTACTCATG GAGGCTGGGT GACCGACCTT 
TGCTTTTCTC CAGACACCAA AATGCTTGTT TCTGCTGGAG GATATCTCAA GTGGTGGAAT 
GTTGTCACTG GGGAATCCTC ACAGACCTTC TACACAAACG GAACCAATCT TAAGAAAATA 
CACGTGTCCC CTGACTTCAA AACATATGTG ACTGTTGATA ATCTTGGTAT TTTATATATT 
CTAAAGATTT TAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803498.1
CDS	469..4086
Translation	MDAKARNCLLQHREALERDIKTSYIMDHMISNGVLTVSEEEKVKNEPTQRQRAAMLIKMILKKDNHSYISFYNALLHEGYKDLAALLHGGIPVISSSDGKDSVSGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLNGEPGWVTIYGMAGCGKSVLAAEAVRDHSFLEDCFPGGVHWVSVGKQDKSGLLMKLQNLCARLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDIWDPWVLKAFDNQCQILLTTRDKSVTDSVMGSPLVVSLIGALLRDFPNRWEYYLRQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDLETEEVEDILQEFVNKSLLFCDRNGKSFLYYLHDLQVDFLAEKNRSQLQDLHKKMVTQFQRHHQLYSLSPDQEDCMYWYSFLAYHMASANMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDGIDCENFQEFLSLNGHLLGRQPFPNIVQLGLCELETSEVYRQAKLQAKQEVDNGMLYLEWVNKKNMKNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSADDKFIATCSVDKKVKIWNSMTGELVRIYDEHSEQVNCCHFTNNSNHLLLATGSNDYFLKLWDLNQEECRNTMFGHTNSVSHCRFSPDDQVLASCSADGTLKLWDVKSANERKSINVKQFFLNSEEPQEDLEVIVKCCSWSADGAKIMVAAKNKVFLFDIHSSGLLAEMHMGHHSAVQYCDFSPHNHLAVVALSQYCVELWSMDSGLKVADCRGHLSWVHCVMFSPDGSSFLTSSDDQTIRLWETKKVCKNSATVLKQEIDVVFQENEVTVLAVDNIRRLQLINGNTGQIDYLTEAQVSCCCLSPHLQYLAFGGEDGAIQILELLNNRIFQSRNGHKTTVRHIQFTADGKTLISSSDDSAVQIWNWQSEDPFFLQAHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITRRIEKDFVCHQDTVLSCDISSDATKFSSTSADKTAKIWSFELLSPLHELRGHKGCVRCSSFSVNNTLLATGDDNGEIRIWNVSNGELLHLCAPISVEEGAATHGGWVTDLCFSPDTKMLVSAGGYLKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILKILE

Target ORF information:

RefSeq Version	XM_010805196.2
Organism	Bos taurus(cattle)
Definition	Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805196.2

ORF Insert Sequence:

ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCCTTGGA AAGGGACATC 
AAAACATCCT ACATTATGGA TCACATGATT AGTAATGGAG TTTTAACAGT GTCAGAGGAG 
GAAAAAGTGA AAAATGAGCC CACCCAACGG CAACGAGCAG CTATGCTAAT TAAAATGATA 
CTTAAAAAAG ATAATCATTC CTATATATCG TTCTACAATG CTCTACTTCA TGAAGGTTAC 
AAAGATCTTG CTGCCCTTCT GCATGGTGGC ATTCCTGTTA TCTCTTCTTC CGATGGCAAA 
GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
CAGAGGCCCG TTGTTTTTGT TACTAGGAAG AAGCTAGTGA ATGCAATTCA GCAGAAGCTC 
TCCAAACTGA ATGGTGAACC AGGATGGGTC ACCATATATG GGATGGCAGG TTGTGGGAAG 
TCTGTATTAG CTGCAGAAGC TGTTCGAGAT CATTCTTTCC TAGAAGATTG TTTCCCAGGA 
GGCGTGCATT GGGTGTCAGT TGGGAAACAG GACAAATCCG GGCTTCTGAT GAAACTACAG 
AACCTTTGTG CACGATTGGA TCAGGATGAG AGCTTCTCCC AGAGGCTTCC ACTTAATATT 
GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTGCGCA AACATCCAAG GTCTTTATTG 
ATCTTGGATG ATATTTGGGA TCCTTGGGTG TTAAAAGCTT TTGACAATCA GTGTCAAATT 
CTTCTCACAA CTAGAGATAA GAGTGTTACA GATTCAGTGA TGGGTTCCCC TCTTGTAGTA 
TCTTTAATTG GTGCACTTTT ACGAGATTTT CCCAACCGCT GGGAGTACTA CCTCAGGCAA 
CTTCAGAATA AGCAATTTAA GAGGATAAGA AAATCTTCAT CTTATGACTA TGAGGCTCTA 
GATGAAGCCA TGTCTATAAG TGTTGAAATG CTCAGAGAAG ACATCAAAGA TTATTACACA 
GATCTTTCCA TCTTACAGAA AGATGTTAAG GTGCCTACAA AGGTGTTATG TATCCTCTGG 
GACTTGGAAA CGGAAGAAGT TGAAGACATA TTACAGGAAT TTGTTAATAA GTCTCTCTTG 
TTCTGTGACC GGAATGGAAA ATCATTTCTT TATTATTTAC ATGATCTGCA AGTAGATTTC 
CTTGCTGAAA AGAATCGCAG CCAGCTTCAG GATCTCCACA AGAAGATGGT CACTCAGTTC 
CAGAGACATC ACCAGCTATA TTCTCTTTCA CCAGATCAGG AGGATTGCAT GTATTGGTAC 
AGTTTTCTGG CCTATCACAT GGCCAGTGCT AATATGCACA AAGAACTGTG TGCTTTAATG 
TTTTCCCTGG ATTGGATTAA AGCAAAAACG GAACTTGTAG GCCCTGCTCA TCTGATTCAT 
GAATTTGTGG AATACAGGCA TATATTGGAT GAAAAGGATG GTATAGACTG TGAGAATTTC 
CAGGAGTTTT TATCTTTAAA TGGACATCTT CTTGGACGAC AGCCATTTCC TAATATTGTG 
CAACTGGGCC TCTGTGAACT GGAAACTTCA GAGGTTTATC GGCAAGCTAA GCTGCAGGCC 
AAGCAGGAGG TCGATAACGG AATGCTTTAC CTGGAGTGGG TAAACAAAAA AAACATGAAG 
AACCTCTCCC GCTTGGTTGT CCGCCCCCAT ACAGATGCTG TTTACCATGC TTGCTTTTCT 
GAAGATGGTC AAAGAATAGC GTCTTGTGGA GCTGATAAAA CTTTACAGGT ATTCAAAGCT 
GAAACAGGAG AGAAACTTCT AGAAATCAAG GCTCATGAGG ATGAAGTGCT TTGCTGTGCA 
TTCTCTGCAG ATGACAAATT TATAGCAACC TGCTCAGTGG ATAAAAAAGT GAAGATTTGG 
AATTCAATGA CTGGGGAACT AGTGCGTATC TATGATGAGC ACTCAGAGCA AGTCAATTGC 
TGCCACTTCA CCAACAATAG TAATCACCTT CTCTTAGCCA CTGGTTCAAA TGACTACTTC 
CTTAAACTCT GGGATCTGAA TCAAGAAGAA TGTCGAAATA CCATGTTTGG CCATACAAAT 
TCGGTCAGTC ACTGCAGATT TTCACCAGAT GATCAGGTTT TGGCCAGTTG TTCAGCTGAT 
GGAACATTAA AGCTTTGGGA TGTGAAATCT GCAAATGAGA GGAAAAGCAT TAATGTGAAG 
CAATTCTTCC TGAATTCAGA GGAGCCTCAG GAGGATCTGG AAGTGATAGT GAAATGCTGT 
TCGTGGTCTG CTGATGGTGC TAAGATAATG GTGGCTGCGA AAAATAAAGT CTTTCTTTTT 
GACATTCACT CCAGTGGCCT GTTGGCAGAA ATGCACATGG GCCATCATAG CGCTGTCCAG 
TACTGTGACT TCTCCCCCCA CAACCATTTG GCAGTGGTGG CTTTGTCCCA GTACTGTGTG 
GAGTTGTGGA GTATGGACTC AGGTTTAAAA GTAGCTGATT GCAGAGGACA TTTAAGTTGG 
GTCCATTGTG TGATGTTTTC TCCTGATGGA TCATCGTTTT TGACATCTTC TGATGACCAG 
ACAATCAGGC TCTGGGAGAC AAAGAAAGTA TGTAAGAACT CTGCTACAGT GTTAAAGCAA 
GAAATAGATG TTGTGTTTCA AGAAAATGAA GTGACGGTTC TTGCAGTTGA CAATATAAGA 
CGTCTGCAAC TCATTAATGG AAACACAGGT CAGATTGATT ACCTAACTGA AGCTCAAGTG 
AGTTGCTGTT GTTTAAGTCC ACATCTTCAG TACCTTGCAT TCGGAGGTGA AGATGGAGCC 
ATTCAGATTT TAGAACTCCT GAACAACAGA ATCTTCCAAT CCAGAAATGG GCACAAGACC 
ACTGTTCGGC ACATCCAGTT CACAGCTGAT GGAAAGACTC TTATCTCAAG CTCTGATGAT 
TCTGCAGTTC AGATATGGAA CTGGCAATCA GAGGACCCTT TCTTTCTCCA AGCCCACCAG 
GAAACAGTGA AAGACTTCAG ACTCTTGAAA AATTCAAGAC TTCTTTCCTG GTCATTTGAT 
GGAACAGTAA AGGTATGGAA TATTATTACC AGAAGAATAG AAAAAGATTT CGTGTGTCAC 
CAGGATACAG TTCTTTCTTG TGATATTTCC TCTGATGCTA CTAAGTTTTC TTCTACCTCT 
GCTGACAAGA CTGCAAAGAT TTGGAGTTTT GAACTCCTTT CTCCTCTTCA TGAATTGAGA 
GGCCACAAAG GCTGTGTGCG CTGCTCTTCC TTCTCTGTGA ACAATACCTT GTTGGCGACT 
GGAGACGACA ATGGAGAAAT CAGGATATGG AATGTCTCAA ACGGTGAGCT TCTTCATTTG 
TGTGCTCCGA TTTCAGTAGA AGAAGGAGCT GCTACTCATG GAGGCTGGGT GACCGACCTT 
TGCTTTTCTC CAGACACCAA AATGCTTGTT TCTGCTGGAG GATATCTCAA GTGGTGGAAT 
GTTGTCACTG GGGAATCCTC ACAGACCTTC TACACAAACG GAACCAATCT TAAGAAAATA 
CACGTGTCCC CTGACTTCAA AACATATGTG ACTGTTGATA ATCTTGGTAT TTTATATATT 
CTAAAGATTT TAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APAF1 ( XM_010805196.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803498.1 Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa53779
Clone ID Related Accession (Same CDS sequence)	XM_010805196.2 , XM_010805196.3
Accession Version	XM_010805196.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	apoptotic protease-activating factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805196.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCCTTGGA AAGGGACATC 
AAAACATCCT ACATTATGGA TCACATGATT AGTAATGGAG TTTTAACAGT GTCAGAGGAG 
GAAAAAGTGA AAAATGAGCC CACCCAACGG CAACGAGCAG CTATGCTAAT TAAAATGATA 
CTTAAAAAAG ATAATCATTC CTATATATCG TTCTACAATG CTCTACTTCA TGAAGGTTAC 
AAAGATCTTG CTGCCCTTCT GCATGGTGGC ATTCCTGTTA TCTCTTCTTC CGATGGCAAA 
GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
CAGAGGCCCG TTGTTTTTGT TACTAGGAAG AAGCTAGTGA ATGCAATTCA GCAGAAGCTC 
TCCAAACTGA ATGGTGAACC AGGATGGGTC ACCATATATG GGATGGCAGG TTGTGGGAAG 
TCTGTATTAG CTGCAGAAGC TGTTCGAGAT CATTCTTTCC TAGAAGATTG TTTCCCAGGA 
GGCGTGCATT GGGTGTCAGT TGGGAAACAG GACAAATCCG GGCTTCTGAT GAAACTACAG 
AACCTTTGTG CACGATTGGA TCAGGATGAG AGCTTCTCCC AGAGGCTTCC ACTTAATATT 
GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTGCGCA AACATCCAAG GTCTTTATTG 
ATCTTGGATG ATATTTGGGA TCCTTGGGTG TTAAAAGCTT TTGACAATCA GTGTCAAATT 
CTTCTCACAA CTAGAGATAA GAGTGTTACA GATTCAGTGA TGGGTTCCCC TCTTGTAGTA 
TCTTTAATTG GTGCACTTTT ACGAGATTTT CCCAACCGCT GGGAGTACTA CCTCAGGCAA 
CTTCAGAATA AGCAATTTAA GAGGATAAGA AAATCTTCAT CTTATGACTA TGAGGCTCTA 
GATGAAGCCA TGTCTATAAG TGTTGAAATG CTCAGAGAAG ACATCAAAGA TTATTACACA 
GATCTTTCCA TCTTACAGAA AGATGTTAAG GTGCCTACAA AGGTGTTATG TATCCTCTGG 
GACTTGGAAA CGGAAGAAGT TGAAGACATA TTACAGGAAT TTGTTAATAA GTCTCTCTTG 
TTCTGTGACC GGAATGGAAA ATCATTTCTT TATTATTTAC ATGATCTGCA AGTAGATTTC 
CTTGCTGAAA AGAATCGCAG CCAGCTTCAG GATCTCCACA AGAAGATGGT CACTCAGTTC 
CAGAGACATC ACCAGCTATA TTCTCTTTCA CCAGATCAGG AGGATTGCAT GTATTGGTAC 
AGTTTTCTGG CCTATCACAT GGCCAGTGCT AATATGCACA AAGAACTGTG TGCTTTAATG 
TTTTCCCTGG ATTGGATTAA AGCAAAAACG GAACTTGTAG GCCCTGCTCA TCTGATTCAT 
GAATTTGTGG AATACAGGCA TATATTGGAT GAAAAGGATG GTATAGACTG TGAGAATTTC 
CAGGAGTTTT TATCTTTAAA TGGACATCTT CTTGGACGAC AGCCATTTCC TAATATTGTG 
CAACTGGGCC TCTGTGAACT GGAAACTTCA GAGGTTTATC GGCAAGCTAA GCTGCAGGCC 
AAGCAGGAGG TCGATAACGG AATGCTTTAC CTGGAGTGGG TAAACAAAAA AAACATGAAG 
AACCTCTCCC GCTTGGTTGT CCGCCCCCAT ACAGATGCTG TTTACCATGC TTGCTTTTCT 
GAAGATGGTC AAAGAATAGC GTCTTGTGGA GCTGATAAAA CTTTACAGGT ATTCAAAGCT 
GAAACAGGAG AGAAACTTCT AGAAATCAAG GCTCATGAGG ATGAAGTGCT TTGCTGTGCA 
TTCTCTGCAG ATGACAAATT TATAGCAACC TGCTCAGTGG ATAAAAAAGT GAAGATTTGG 
AATTCAATGA CTGGGGAACT AGTGCGTATC TATGATGAGC ACTCAGAGCA AGTCAATTGC 
TGCCACTTCA CCAACAATAG TAATCACCTT CTCTTAGCCA CTGGTTCAAA TGACTACTTC 
CTTAAACTCT GGGATCTGAA TCAAGAAGAA TGTCGAAATA CCATGTTTGG CCATACAAAT 
TCGGTCAGTC ACTGCAGATT TTCACCAGAT GATCAGGTTT TGGCCAGTTG TTCAGCTGAT 
GGAACATTAA AGCTTTGGGA TGTGAAATCT GCAAATGAGA GGAAAAGCAT TAATGTGAAG 
CAATTCTTCC TGAATTCAGA GGAGCCTCAG GAGGATCTGG AAGTGATAGT GAAATGCTGT 
TCGTGGTCTG CTGATGGTGC TAAGATAATG GTGGCTGCGA AAAATAAAGT CTTTCTTTTT 
GACATTCACT CCAGTGGCCT GTTGGCAGAA ATGCACATGG GCCATCATAG CGCTGTCCAG 
TACTGTGACT TCTCCCCCCA CAACCATTTG GCAGTGGTGG CTTTGTCCCA GTACTGTGTG 
GAGTTGTGGA GTATGGACTC AGGTTTAAAA GTAGCTGATT GCAGAGGACA TTTAAGTTGG 
GTCCATTGTG TGATGTTTTC TCCTGATGGA TCATCGTTTT TGACATCTTC TGATGACCAG 
ACAATCAGGC TCTGGGAGAC AAAGAAAGTA TGTAAGAACT CTGCTACAGT GTTAAAGCAA 
GAAATAGATG TTGTGTTTCA AGAAAATGAA GTGACGGTTC TTGCAGTTGA CAATATAAGA 
CGTCTGCAAC TCATTAATGG AAACACAGGT CAGATTGATT ACCTAACTGA AGCTCAAGTG 
AGTTGCTGTT GTTTAAGTCC ACATCTTCAG TACCTTGCAT TCGGAGGTGA AGATGGAGCC 
ATTCAGATTT TAGAACTCCT GAACAACAGA ATCTTCCAAT CCAGAAATGG GCACAAGACC 
ACTGTTCGGC ACATCCAGTT CACAGCTGAT GGAAAGACTC TTATCTCAAG CTCTGATGAT 
TCTGCAGTTC AGATATGGAA CTGGCAATCA GAGGACCCTT TCTTTCTCCA AGCCCACCAG 
GAAACAGTGA AAGACTTCAG ACTCTTGAAA AATTCAAGAC TTCTTTCCTG GTCATTTGAT 
GGAACAGTAA AGGTATGGAA TATTATTACC AGAAGAATAG AAAAAGATTT CGTGTGTCAC 
CAGGATACAG TTCTTTCTTG TGATATTTCC TCTGATGCTA CTAAGTTTTC TTCTACCTCT 
GCTGACAAGA CTGCAAAGAT TTGGAGTTTT GAACTCCTTT CTCCTCTTCA TGAATTGAGA 
GGCCACAAAG GCTGTGTGCG CTGCTCTTCC TTCTCTGTGA ACAATACCTT GTTGGCGACT 
GGAGACGACA ATGGAGAAAT CAGGATATGG AATGTCTCAA ACGGTGAGCT TCTTCATTTG 
TGTGCTCCGA TTTCAGTAGA AGAAGGAGCT GCTACTCATG GAGGCTGGGT GACCGACCTT 
TGCTTTTCTC CAGACACCAA AATGCTTGTT TCTGCTGGAG GATATCTCAA GTGGTGGAAT 
GTTGTCACTG GGGAATCCTC ACAGACCTTC TACACAAACG GAACCAATCT TAAGAAAATA 
CACGTGTCCC CTGACTTCAA AACATATGTG ACTGTTGATA ATCTTGGTAT TTTATATATT 
CTAAAGATTT TAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803498.1
CDS	498..4115
Translation	MDAKARNCLLQHREALERDIKTSYIMDHMISNGVLTVSEEEKVKNEPTQRQRAAMLIKMILKKDNHSYISFYNALLHEGYKDLAALLHGGIPVISSSDGKDSVSGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLNGEPGWVTIYGMAGCGKSVLAAEAVRDHSFLEDCFPGGVHWVSVGKQDKSGLLMKLQNLCARLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDIWDPWVLKAFDNQCQILLTTRDKSVTDSVMGSPLVVSLIGALLRDFPNRWEYYLRQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDLETEEVEDILQEFVNKSLLFCDRNGKSFLYYLHDLQVDFLAEKNRSQLQDLHKKMVTQFQRHHQLYSLSPDQEDCMYWYSFLAYHMASANMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDGIDCENFQEFLSLNGHLLGRQPFPNIVQLGLCELETSEVYRQAKLQAKQEVDNGMLYLEWVNKKNMKNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSADDKFIATCSVDKKVKIWNSMTGELVRIYDEHSEQVNCCHFTNNSNHLLLATGSNDYFLKLWDLNQEECRNTMFGHTNSVSHCRFSPDDQVLASCSADGTLKLWDVKSANERKSINVKQFFLNSEEPQEDLEVIVKCCSWSADGAKIMVAAKNKVFLFDIHSSGLLAEMHMGHHSAVQYCDFSPHNHLAVVALSQYCVELWSMDSGLKVADCRGHLSWVHCVMFSPDGSSFLTSSDDQTIRLWETKKVCKNSATVLKQEIDVVFQENEVTVLAVDNIRRLQLINGNTGQIDYLTEAQVSCCCLSPHLQYLAFGGEDGAIQILELLNNRIFQSRNGHKTTVRHIQFTADGKTLISSSDDSAVQIWNWQSEDPFFLQAHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITRRIEKDFVCHQDTVLSCDISSDATKFSSTSADKTAKIWSFELLSPLHELRGHKGCVRCSSFSVNNTLLATGDDNGEIRIWNVSNGELLHLCAPISVEEGAATHGGWVTDLCFSPDTKMLVSAGGYLKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILKILE

Target ORF information:

RefSeq Version	XM_010805196.3
Organism	Bos taurus(cattle)
Definition	Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805196.3

ORF Insert Sequence:

ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCCTTGGA AAGGGACATC 
AAAACATCCT ACATTATGGA TCACATGATT AGTAATGGAG TTTTAACAGT GTCAGAGGAG 
GAAAAAGTGA AAAATGAGCC CACCCAACGG CAACGAGCAG CTATGCTAAT TAAAATGATA 
CTTAAAAAAG ATAATCATTC CTATATATCG TTCTACAATG CTCTACTTCA TGAAGGTTAC 
AAAGATCTTG CTGCCCTTCT GCATGGTGGC ATTCCTGTTA TCTCTTCTTC CGATGGCAAA 
GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
CAGAGGCCCG TTGTTTTTGT TACTAGGAAG AAGCTAGTGA ATGCAATTCA GCAGAAGCTC 
TCCAAACTGA ATGGTGAACC AGGATGGGTC ACCATATATG GGATGGCAGG TTGTGGGAAG 
TCTGTATTAG CTGCAGAAGC TGTTCGAGAT CATTCTTTCC TAGAAGATTG TTTCCCAGGA 
GGCGTGCATT GGGTGTCAGT TGGGAAACAG GACAAATCCG GGCTTCTGAT GAAACTACAG 
AACCTTTGTG CACGATTGGA TCAGGATGAG AGCTTCTCCC AGAGGCTTCC ACTTAATATT 
GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTGCGCA AACATCCAAG GTCTTTATTG 
ATCTTGGATG ATATTTGGGA TCCTTGGGTG TTAAAAGCTT TTGACAATCA GTGTCAAATT 
CTTCTCACAA CTAGAGATAA GAGTGTTACA GATTCAGTGA TGGGTTCCCC TCTTGTAGTA 
TCTTTAATTG GTGCACTTTT ACGAGATTTT CCCAACCGCT GGGAGTACTA CCTCAGGCAA 
CTTCAGAATA AGCAATTTAA GAGGATAAGA AAATCTTCAT CTTATGACTA TGAGGCTCTA 
GATGAAGCCA TGTCTATAAG TGTTGAAATG CTCAGAGAAG ACATCAAAGA TTATTACACA 
GATCTTTCCA TCTTACAGAA AGATGTTAAG GTGCCTACAA AGGTGTTATG TATCCTCTGG 
GACTTGGAAA CGGAAGAAGT TGAAGACATA TTACAGGAAT TTGTTAATAA GTCTCTCTTG 
TTCTGTGACC GGAATGGAAA ATCATTTCTT TATTATTTAC ATGATCTGCA AGTAGATTTC 
CTTGCTGAAA AGAATCGCAG CCAGCTTCAG GATCTCCACA AGAAGATGGT CACTCAGTTC 
CAGAGACATC ACCAGCTATA TTCTCTTTCA CCAGATCAGG AGGATTGCAT GTATTGGTAC 
AGTTTTCTGG CCTATCACAT GGCCAGTGCT AATATGCACA AAGAACTGTG TGCTTTAATG 
TTTTCCCTGG ATTGGATTAA AGCAAAAACG GAACTTGTAG GCCCTGCTCA TCTGATTCAT 
GAATTTGTGG AATACAGGCA TATATTGGAT GAAAAGGATG GTATAGACTG TGAGAATTTC 
CAGGAGTTTT TATCTTTAAA TGGACATCTT CTTGGACGAC AGCCATTTCC TAATATTGTG 
CAACTGGGCC TCTGTGAACT GGAAACTTCA GAGGTTTATC GGCAAGCTAA GCTGCAGGCC 
AAGCAGGAGG TCGATAACGG AATGCTTTAC CTGGAGTGGG TAAACAAAAA AAACATGAAG 
AACCTCTCCC GCTTGGTTGT CCGCCCCCAT ACAGATGCTG TTTACCATGC TTGCTTTTCT 
GAAGATGGTC AAAGAATAGC GTCTTGTGGA GCTGATAAAA CTTTACAGGT ATTCAAAGCT 
GAAACAGGAG AGAAACTTCT AGAAATCAAG GCTCATGAGG ATGAAGTGCT TTGCTGTGCA 
TTCTCTGCAG ATGACAAATT TATAGCAACC TGCTCAGTGG ATAAAAAAGT GAAGATTTGG 
AATTCAATGA CTGGGGAACT AGTGCGTATC TATGATGAGC ACTCAGAGCA AGTCAATTGC 
TGCCACTTCA CCAACAATAG TAATCACCTT CTCTTAGCCA CTGGTTCAAA TGACTACTTC 
CTTAAACTCT GGGATCTGAA TCAAGAAGAA TGTCGAAATA CCATGTTTGG CCATACAAAT 
TCGGTCAGTC ACTGCAGATT TTCACCAGAT GATCAGGTTT TGGCCAGTTG TTCAGCTGAT 
GGAACATTAA AGCTTTGGGA TGTGAAATCT GCAAATGAGA GGAAAAGCAT TAATGTGAAG 
CAATTCTTCC TGAATTCAGA GGAGCCTCAG GAGGATCTGG AAGTGATAGT GAAATGCTGT 
TCGTGGTCTG CTGATGGTGC TAAGATAATG GTGGCTGCGA AAAATAAAGT CTTTCTTTTT 
GACATTCACT CCAGTGGCCT GTTGGCAGAA ATGCACATGG GCCATCATAG CGCTGTCCAG 
TACTGTGACT TCTCCCCCCA CAACCATTTG GCAGTGGTGG CTTTGTCCCA GTACTGTGTG 
GAGTTGTGGA GTATGGACTC AGGTTTAAAA GTAGCTGATT GCAGAGGACA TTTAAGTTGG 
GTCCATTGTG TGATGTTTTC TCCTGATGGA TCATCGTTTT TGACATCTTC TGATGACCAG 
ACAATCAGGC TCTGGGAGAC AAAGAAAGTA TGTAAGAACT CTGCTACAGT GTTAAAGCAA 
GAAATAGATG TTGTGTTTCA AGAAAATGAA GTGACGGTTC TTGCAGTTGA CAATATAAGA 
CGTCTGCAAC TCATTAATGG AAACACAGGT CAGATTGATT ACCTAACTGA AGCTCAAGTG 
AGTTGCTGTT GTTTAAGTCC ACATCTTCAG TACCTTGCAT TCGGAGGTGA AGATGGAGCC 
ATTCAGATTT TAGAACTCCT GAACAACAGA ATCTTCCAAT CCAGAAATGG GCACAAGACC 
ACTGTTCGGC ACATCCAGTT CACAGCTGAT GGAAAGACTC TTATCTCAAG CTCTGATGAT 
TCTGCAGTTC AGATATGGAA CTGGCAATCA GAGGACCCTT TCTTTCTCCA AGCCCACCAG 
GAAACAGTGA AAGACTTCAG ACTCTTGAAA AATTCAAGAC TTCTTTCCTG GTCATTTGAT 
GGAACAGTAA AGGTATGGAA TATTATTACC AGAAGAATAG AAAAAGATTT CGTGTGTCAC 
CAGGATACAG TTCTTTCTTG TGATATTTCC TCTGATGCTA CTAAGTTTTC TTCTACCTCT 
GCTGACAAGA CTGCAAAGAT TTGGAGTTTT GAACTCCTTT CTCCTCTTCA TGAATTGAGA 
GGCCACAAAG GCTGTGTGCG CTGCTCTTCC TTCTCTGTGA ACAATACCTT GTTGGCGACT 
GGAGACGACA ATGGAGAAAT CAGGATATGG AATGTCTCAA ACGGTGAGCT TCTTCATTTG 
TGTGCTCCGA TTTCAGTAGA AGAAGGAGCT GCTACTCATG GAGGCTGGGT GACCGACCTT 
TGCTTTTCTC CAGACACCAA AATGCTTGTT TCTGCTGGAG GATATCTCAA GTGGTGGAAT 
GTTGTCACTG GGGAATCCTC ACAGACCTTC TACACAAACG GAACCAATCT TAAGAAAATA 
CACGTGTCCC CTGACTTCAA AACATATGTG ACTGTTGATA ATCTTGGTAT TTTATATATT 
CTAAAGATTT TAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APAF1 ( XM_010805198.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803500.1 Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa53781
Clone ID Related Accession (Same CDS sequence)	XM_010805198.3 , XM_010805198.2
Accession Version	XM_010805198.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	apoptotic protease-activating factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010805198.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAA TACGTGATTG TTTCCCAGGA GGCGTGCATT GGGTGTCAGT TGGGAAACAG 
GACAAATCCG GGCTTCTGAT GAAACTACAG AACCTTTGTG CACGATTGGA TCAGGATGAG 
AGCTTCTCCC AGAGGCTTCC ACTTAATATT GAAGAGGCTA AAGACCGTCT CCGCATTCTG 
ATGCTGCGCA AACATCCAAG GTCTTTATTG ATCTTGGATG ATATTTGGGA TCCTTGGGTG 
TTAAAAGCTT TTGACAATCA GTGTCAAATT CTTCTCACAA CTAGAGATAA GAGTGTTACA 
GATTCAGTGA TGGGTCCTAA ATATGTAGTG CCTGTGGAGA GTGGTTTAGG AAAAGAAAAA 
GGACTTGAAA TTTTATCCCT TTTTGTTAAT ATGAAGAAAG CAGATTTGCC AGAACAAGCT 
CACAGTATTA TACAAGAATG TAAAGGTTCC CCTCTTGTAG TATCTTTAAT TGGTGCACTT 
TTACGAGATT TTCCCAACCG CTGGGAGTAC TACCTCAGGC AACTTCAGAA TAAGCAATTT 
AAGAGGATAA GAAAATCTTC ATCTTATGAC TATGAGGCTC TAGATGAAGC CATGTCTATA 
AGTGTTGAAA TGCTCAGAGA AGACATCAAA GATTATTACA CAGATCTTTC CATCTTACAG 
AAAGATGTTA AGGTGCCTAC AAAGGTGTTA TGTATCCTCT GGGACTTGGA AACGGAAGAA 
GTTGAAGACA TATTACAGGA ATTTGTTAAT AAGTCTCTCT TGTTCTGTGA CCGGAATGGA 
AAATCATTTC TTTATTATTT ACATGATCTG CAAGTAGATT TCCTTGCTGA AAAGAATCGC 
AGCCAGCTTC AGGATCTCCA CAAGAAGATG GTCACTCAGT TCCAGAGACA TCACCAGCTA 
TATTCTCTTT CACCAGATCA GGAGGATTGC ATGTATTGGT ACAGTTTTCT GGCCTATCAC 
ATGGCCAGTG CTAATATGCA CAAAGAACTG TGTGCTTTAA TGTTTTCCCT GGATTGGATT 
AAAGCAAAAA CGGAACTTGT AGGCCCTGCT CATCTGATTC ATGAATTTGT GGAATACAGG 
CATATATTGG ATGAAAAGGA TGGTATAGAC TGTGAGAATT TCCAGGAGTT TTTATCTTTA 
AATGGACATC TTCTTGGACG ACAGCCATTT CCTAATATTG TGCAACTGGG CCTCTGTGAA 
CTGGAAACTT CAGAGGTTTA TCGGCAAGCT AAGCTGCAGG CCAAGCAGGA GGTCGATAAC 
GGAATGCTTT ACCTGGAGTG GGTAAACAAA AAAAACATGA AGAACCTCTC CCGCTTGGTT 
GTCCGCCCCC ATACAGATGC TGTTTACCAT GCTTGCTTTT CTGAAGATGG TCAAAGAATA 
GCGTCTTGTG GAGCTGATAA AACTTTACAG GTATTCAAAG CTGAAACAGG AGAGAAACTT 
CTAGAAATCA AGGCTCATGA GGATGAAGTG CTTTGCTGTG CATTCTCTGC AGATGACAAA 
TTTATAGCAA CCTGCTCAGT GGATAAAAAA GTGAAGATTT GGAATTCAAT GACTGGGGAA 
CTAGTGCGTA TCTATGATGA GCACTCAGAG CAAGTCAATT GCTGCCACTT CACCAACAAT 
AGTAATCACC TTCTCTTAGC CACTGGTTCA AATGACTACT TCCTTAAACT CTGGGATCTG 
AATCAAGAAG AATGTCGAAA TACCATGTTT GGCCATACAA ATTCGGTCAG TCACTGCAGA 
TTTTCACCAG ATGATCAGGT TTTGGCCAGT TGTTCAGCTG ATGGAACATT AAAGCTTTGG 
GATGTGAAAT CTGCAAATGA GAGGAAAAGC ATTAATGTGA AGCAATTCTT CCTGAATTCA 
GAGGAGCCTC AGGAGGATCT GGAAGTGATA GTGAAATGCT GTTCGTGGTC TGCTGATGGT 
GCTAAGATAA TGGTGGCTGC GAAAAATAAA GTCTTTCTTT TTGACATTCA CTCCAGTGGC 
CTGTTGGCAG AAATGCACAT GGGCCATCAT AGCGCTGTCC AGTACTGTGA CTTCTCCCCC 
CACAACCATT TGGCAGTGGT GGCTTTGTCC CAGTACTGTG TGGAGTTGTG GAGTATGGAC 
TCAGGTTTAA AAGTAGCTGA TTGCAGAGGA CATTTAAGTT GGGTCCATTG TGTGATGTTT 
TCTCCTGATG GATCATCGTT TTTGACATCT TCTGATGACC AGACAATCAG GCTCTGGGAG 
ACAAAGAAAG TATGTAAGAA CTCTGCTACA GTGTTAAAGC AAGAAATAGA TGTTGTGTTT 
CAAGAAAATG AAGTGACGGT TCTTGCAGTT GACAATATAA GACGTCTGCA ACTCATTAAT 
GGAAACACAG GTCAGATTGA TTACCTAACT GAAGCTCAAG TGAGTTGCTG TTGTTTAAGT 
CCACATCTTC AGTACCTTGC ATTCGGAGGT GAAGATGGAG CCATTCAGAT TTTAGAACTC 
CTGAACAACA GAATCTTCCA ATCCAGAAAT GGGCACAAGA CCACTGTTCG GCACATCCAG 
TTCACAGCTG ATGGAAAGAC TCTTATCTCA AGCTCTGATG ATTCTGCAGT TCAGATATGG 
AACTGGCAAT CAGAGGACCC TTTCTTTCTC CAAGCCCACC AGGAAACAGT GAAAGACTTC 
AGACTCTTGA AAAATTCAAG ACTTCTTTCC TGGTCATTTG ATGGAACAGT AAAGGTATGG 
AATATTATTA CCAGAAGAAT AGAAAAAGAT TTCGTGTGTC ACCAGGATAC AGTTCTTTCT 
TGTGATATTT CCTCTGATGC TACTAAGTTT TCTTCTACCT CTGCTGACAA GACTGCAAAG 
ATTTGGAGTT TTGAACTCCT TTCTCCTCTT CATGAATTGA GAGGCCACAA AGGCTGTGTG 
CGCTGCTCTT CCTTCTCTGT GAACAATACC TTGTTGGCGA CTGGAGACGA CAATGGAGAA 
ATCAGGATAT GGAATGTCTC AAACGGTGAG CTTCTTCATT TGTGTGCTCC GATTTCAGTA 
GAAGAAGGAG CTGCTACTCA TGGAGGCTGG GTGACCGACC TTTGCTTTTC TCCAGACACC 
AAAATGCTTG TTTCTGCTGG AGGATATCTC AAGTGGTGGA ATGTTGTCAC TGGGGAATCC 
TCACAGACCT TCTACACAAA CGGAACCAAT CTTAAGAAAA TACACGTGTC CCCTGACTTC 
AAAACATATG TGACTGTTGA TAATCTTGGT ATTTTATATA TTCTAAAGAT TTTAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803500.1
CDS	122..3361
Translation	MSGIRDCFPGGVHWVSVGKQDKSGLLMKLQNLCARLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDIWDPWVLKAFDNQCQILLTTRDKSVTDSVMGPKYVVPVESGLGKEKGLEILSLFVNMKKADLPEQAHSIIQECKGSPLVVSLIGALLRDFPNRWEYYLRQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDLETEEVEDILQEFVNKSLLFCDRNGKSFLYYLHDLQVDFLAEKNRSQLQDLHKKMVTQFQRHHQLYSLSPDQEDCMYWYSFLAYHMASANMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDGIDCENFQEFLSLNGHLLGRQPFPNIVQLGLCELETSEVYRQAKLQAKQEVDNGMLYLEWVNKKNMKNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSADDKFIATCSVDKKVKIWNSMTGELVRIYDEHSEQVNCCHFTNNSNHLLLATGSNDYFLKLWDLNQEECRNTMFGHTNSVSHCRFSPDDQVLASCSADGTLKLWDVKSANERKSINVKQFFLNSEEPQEDLEVIVKCCSWSADGAKIMVAAKNKVFLFDIHSSGLLAEMHMGHHSAVQYCDFSPHNHLAVVALSQYCVELWSMDSGLKVADCRGHLSWVHCVMFSPDGSSFLTSSDDQTIRLWETKKVCKNSATVLKQEIDVVFQENEVTVLAVDNIRRLQLINGNTGQIDYLTEAQVSCCCLSPHLQYLAFGGEDGAIQILELLNNRIFQSRNGHKTTVRHIQFTADGKTLISSSDDSAVQIWNWQSEDPFFLQAHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITRRIEKDFVCHQDTVLSCDISSDATKFSSTSADKTAKIWSFELLSPLHELRGHKGCVRCSSFSVNNTLLATGDDNGEIRIWNVSNGELLHLCAPISVEEGAATHGGWVTDLCFSPDTKMLVSAGGYLKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILKILE

Target ORF information:

RefSeq Version	XM_010805198.3
Organism	Bos taurus(cattle)
Definition	Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805198.3

ORF Insert Sequence:

ATGAGTGGAA TACGTGATTG TTTCCCAGGA GGCGTGCATT GGGTGTCAGT TGGGAAACAG 
GACAAATCCG GGCTTCTGAT GAAACTACAG AACCTTTGTG CACGATTGGA TCAGGATGAG 
AGCTTCTCCC AGAGGCTTCC ACTTAATATT GAAGAGGCTA AAGACCGTCT CCGCATTCTG 
ATGCTGCGCA AACATCCAAG GTCTTTATTG ATCTTGGATG ATATTTGGGA TCCTTGGGTG 
TTAAAAGCTT TTGACAATCA GTGTCAAATT CTTCTCACAA CTAGAGATAA GAGTGTTACA 
GATTCAGTGA TGGGTCCTAA ATATGTAGTG CCTGTGGAGA GTGGTTTAGG AAAAGAAAAA 
GGACTTGAAA TTTTATCCCT TTTTGTTAAT ATGAAGAAAG CAGATTTGCC AGAACAAGCT 
CACAGTATTA TACAAGAATG TAAAGGTTCC CCTCTTGTAG TATCTTTAAT TGGTGCACTT 
TTACGAGATT TTCCCAACCG CTGGGAGTAC TACCTCAGGC AACTTCAGAA TAAGCAATTT 
AAGAGGATAA GAAAATCTTC ATCTTATGAC TATGAGGCTC TAGATGAAGC CATGTCTATA 
AGTGTTGAAA TGCTCAGAGA AGACATCAAA GATTATTACA CAGATCTTTC CATCTTACAG 
AAAGATGTTA AGGTGCCTAC AAAGGTGTTA TGTATCCTCT GGGACTTGGA AACGGAAGAA 
GTTGAAGACA TATTACAGGA ATTTGTTAAT AAGTCTCTCT TGTTCTGTGA CCGGAATGGA 
AAATCATTTC TTTATTATTT ACATGATCTG CAAGTAGATT TCCTTGCTGA AAAGAATCGC 
AGCCAGCTTC AGGATCTCCA CAAGAAGATG GTCACTCAGT TCCAGAGACA TCACCAGCTA 
TATTCTCTTT CACCAGATCA GGAGGATTGC ATGTATTGGT ACAGTTTTCT GGCCTATCAC 
ATGGCCAGTG CTAATATGCA CAAAGAACTG TGTGCTTTAA TGTTTTCCCT GGATTGGATT 
AAAGCAAAAA CGGAACTTGT AGGCCCTGCT CATCTGATTC ATGAATTTGT GGAATACAGG 
CATATATTGG ATGAAAAGGA TGGTATAGAC TGTGAGAATT TCCAGGAGTT TTTATCTTTA 
AATGGACATC TTCTTGGACG ACAGCCATTT CCTAATATTG TGCAACTGGG CCTCTGTGAA 
CTGGAAACTT CAGAGGTTTA TCGGCAAGCT AAGCTGCAGG CCAAGCAGGA GGTCGATAAC 
GGAATGCTTT ACCTGGAGTG GGTAAACAAA AAAAACATGA AGAACCTCTC CCGCTTGGTT 
GTCCGCCCCC ATACAGATGC TGTTTACCAT GCTTGCTTTT CTGAAGATGG TCAAAGAATA 
GCGTCTTGTG GAGCTGATAA AACTTTACAG GTATTCAAAG CTGAAACAGG AGAGAAACTT 
CTAGAAATCA AGGCTCATGA GGATGAAGTG CTTTGCTGTG CATTCTCTGC AGATGACAAA 
TTTATAGCAA CCTGCTCAGT GGATAAAAAA GTGAAGATTT GGAATTCAAT GACTGGGGAA 
CTAGTGCGTA TCTATGATGA GCACTCAGAG CAAGTCAATT GCTGCCACTT CACCAACAAT 
AGTAATCACC TTCTCTTAGC CACTGGTTCA AATGACTACT TCCTTAAACT CTGGGATCTG 
AATCAAGAAG AATGTCGAAA TACCATGTTT GGCCATACAA ATTCGGTCAG TCACTGCAGA 
TTTTCACCAG ATGATCAGGT TTTGGCCAGT TGTTCAGCTG ATGGAACATT AAAGCTTTGG 
GATGTGAAAT CTGCAAATGA GAGGAAAAGC ATTAATGTGA AGCAATTCTT CCTGAATTCA 
GAGGAGCCTC AGGAGGATCT GGAAGTGATA GTGAAATGCT GTTCGTGGTC TGCTGATGGT 
GCTAAGATAA TGGTGGCTGC GAAAAATAAA GTCTTTCTTT TTGACATTCA CTCCAGTGGC 
CTGTTGGCAG AAATGCACAT GGGCCATCAT AGCGCTGTCC AGTACTGTGA CTTCTCCCCC 
CACAACCATT TGGCAGTGGT GGCTTTGTCC CAGTACTGTG TGGAGTTGTG GAGTATGGAC 
TCAGGTTTAA AAGTAGCTGA TTGCAGAGGA CATTTAAGTT GGGTCCATTG TGTGATGTTT 
TCTCCTGATG GATCATCGTT TTTGACATCT TCTGATGACC AGACAATCAG GCTCTGGGAG 
ACAAAGAAAG TATGTAAGAA CTCTGCTACA GTGTTAAAGC AAGAAATAGA TGTTGTGTTT 
CAAGAAAATG AAGTGACGGT TCTTGCAGTT GACAATATAA GACGTCTGCA ACTCATTAAT 
GGAAACACAG GTCAGATTGA TTACCTAACT GAAGCTCAAG TGAGTTGCTG TTGTTTAAGT 
CCACATCTTC AGTACCTTGC ATTCGGAGGT GAAGATGGAG CCATTCAGAT TTTAGAACTC 
CTGAACAACA GAATCTTCCA ATCCAGAAAT GGGCACAAGA CCACTGTTCG GCACATCCAG 
TTCACAGCTG ATGGAAAGAC TCTTATCTCA AGCTCTGATG ATTCTGCAGT TCAGATATGG 
AACTGGCAAT CAGAGGACCC TTTCTTTCTC CAAGCCCACC AGGAAACAGT GAAAGACTTC 
AGACTCTTGA AAAATTCAAG ACTTCTTTCC TGGTCATTTG ATGGAACAGT AAAGGTATGG 
AATATTATTA CCAGAAGAAT AGAAAAAGAT TTCGTGTGTC ACCAGGATAC AGTTCTTTCT 
TGTGATATTT CCTCTGATGC TACTAAGTTT TCTTCTACCT CTGCTGACAA GACTGCAAAG 
ATTTGGAGTT TTGAACTCCT TTCTCCTCTT CATGAATTGA GAGGCCACAA AGGCTGTGTG 
CGCTGCTCTT CCTTCTCTGT GAACAATACC TTGTTGGCGA CTGGAGACGA CAATGGAGAA 
ATCAGGATAT GGAATGTCTC AAACGGTGAG CTTCTTCATT TGTGTGCTCC GATTTCAGTA 
GAAGAAGGAG CTGCTACTCA TGGAGGCTGG GTGACCGACC TTTGCTTTTC TCCAGACACC 
AAAATGCTTG TTTCTGCTGG AGGATATCTC AAGTGGTGGA ATGTTGTCAC TGGGGAATCC 
TCACAGACCT TCTACACAAA CGGAACCAAT CTTAAGAAAA TACACGTGTC CCCTGACTTC 
AAAACATATG TGACTGTTGA TAATCTTGGT ATTTTATATA TTCTAAAGAT TTTAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APAF1 ( XM_010805198.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010803500.1 Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa53781
Clone ID Related Accession (Same CDS sequence)	XM_010805198.3 , XM_010805198.2
Accession Version	XM_010805198.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	apoptotic protease-activating factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103925.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010805198.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTGGAA TACGTGATTG TTTCCCAGGA GGCGTGCATT GGGTGTCAGT TGGGAAACAG 
GACAAATCCG GGCTTCTGAT GAAACTACAG AACCTTTGTG CACGATTGGA TCAGGATGAG 
AGCTTCTCCC AGAGGCTTCC ACTTAATATT GAAGAGGCTA AAGACCGTCT CCGCATTCTG 
ATGCTGCGCA AACATCCAAG GTCTTTATTG ATCTTGGATG ATATTTGGGA TCCTTGGGTG 
TTAAAAGCTT TTGACAATCA GTGTCAAATT CTTCTCACAA CTAGAGATAA GAGTGTTACA 
GATTCAGTGA TGGGTCCTAA ATATGTAGTG CCTGTGGAGA GTGGTTTAGG AAAAGAAAAA 
GGACTTGAAA TTTTATCCCT TTTTGTTAAT ATGAAGAAAG CAGATTTGCC AGAACAAGCT 
CACAGTATTA TACAAGAATG TAAAGGTTCC CCTCTTGTAG TATCTTTAAT TGGTGCACTT 
TTACGAGATT TTCCCAACCG CTGGGAGTAC TACCTCAGGC AACTTCAGAA TAAGCAATTT 
AAGAGGATAA GAAAATCTTC ATCTTATGAC TATGAGGCTC TAGATGAAGC CATGTCTATA 
AGTGTTGAAA TGCTCAGAGA AGACATCAAA GATTATTACA CAGATCTTTC CATCTTACAG 
AAAGATGTTA AGGTGCCTAC AAAGGTGTTA TGTATCCTCT GGGACTTGGA AACGGAAGAA 
GTTGAAGACA TATTACAGGA ATTTGTTAAT AAGTCTCTCT TGTTCTGTGA CCGGAATGGA 
AAATCATTTC TTTATTATTT ACATGATCTG CAAGTAGATT TCCTTGCTGA AAAGAATCGC 
AGCCAGCTTC AGGATCTCCA CAAGAAGATG GTCACTCAGT TCCAGAGACA TCACCAGCTA 
TATTCTCTTT CACCAGATCA GGAGGATTGC ATGTATTGGT ACAGTTTTCT GGCCTATCAC 
ATGGCCAGTG CTAATATGCA CAAAGAACTG TGTGCTTTAA TGTTTTCCCT GGATTGGATT 
AAAGCAAAAA CGGAACTTGT AGGCCCTGCT CATCTGATTC ATGAATTTGT GGAATACAGG 
CATATATTGG ATGAAAAGGA TGGTATAGAC TGTGAGAATT TCCAGGAGTT TTTATCTTTA 
AATGGACATC TTCTTGGACG ACAGCCATTT CCTAATATTG TGCAACTGGG CCTCTGTGAA 
CTGGAAACTT CAGAGGTTTA TCGGCAAGCT AAGCTGCAGG CCAAGCAGGA GGTCGATAAC 
GGAATGCTTT ACCTGGAGTG GGTAAACAAA AAAAACATGA AGAACCTCTC CCGCTTGGTT 
GTCCGCCCCC ATACAGATGC TGTTTACCAT GCTTGCTTTT CTGAAGATGG TCAAAGAATA 
GCGTCTTGTG GAGCTGATAA AACTTTACAG GTATTCAAAG CTGAAACAGG AGAGAAACTT 
CTAGAAATCA AGGCTCATGA GGATGAAGTG CTTTGCTGTG CATTCTCTGC AGATGACAAA 
TTTATAGCAA CCTGCTCAGT GGATAAAAAA GTGAAGATTT GGAATTCAAT GACTGGGGAA 
CTAGTGCGTA TCTATGATGA GCACTCAGAG CAAGTCAATT GCTGCCACTT CACCAACAAT 
AGTAATCACC TTCTCTTAGC CACTGGTTCA AATGACTACT TCCTTAAACT CTGGGATCTG 
AATCAAGAAG AATGTCGAAA TACCATGTTT GGCCATACAA ATTCGGTCAG TCACTGCAGA 
TTTTCACCAG ATGATCAGGT TTTGGCCAGT TGTTCAGCTG ATGGAACATT AAAGCTTTGG 
GATGTGAAAT CTGCAAATGA GAGGAAAAGC ATTAATGTGA AGCAATTCTT CCTGAATTCA 
GAGGAGCCTC AGGAGGATCT GGAAGTGATA GTGAAATGCT GTTCGTGGTC TGCTGATGGT 
GCTAAGATAA TGGTGGCTGC GAAAAATAAA GTCTTTCTTT TTGACATTCA CTCCAGTGGC 
CTGTTGGCAG AAATGCACAT GGGCCATCAT AGCGCTGTCC AGTACTGTGA CTTCTCCCCC 
CACAACCATT TGGCAGTGGT GGCTTTGTCC CAGTACTGTG TGGAGTTGTG GAGTATGGAC 
TCAGGTTTAA AAGTAGCTGA TTGCAGAGGA CATTTAAGTT GGGTCCATTG TGTGATGTTT 
TCTCCTGATG GATCATCGTT TTTGACATCT TCTGATGACC AGACAATCAG GCTCTGGGAG 
ACAAAGAAAG TATGTAAGAA CTCTGCTACA GTGTTAAAGC AAGAAATAGA TGTTGTGTTT 
CAAGAAAATG AAGTGACGGT TCTTGCAGTT GACAATATAA GACGTCTGCA ACTCATTAAT 
GGAAACACAG GTCAGATTGA TTACCTAACT GAAGCTCAAG TGAGTTGCTG TTGTTTAAGT 
CCACATCTTC AGTACCTTGC ATTCGGAGGT GAAGATGGAG CCATTCAGAT TTTAGAACTC 
CTGAACAACA GAATCTTCCA ATCCAGAAAT GGGCACAAGA CCACTGTTCG GCACATCCAG 
TTCACAGCTG ATGGAAAGAC TCTTATCTCA AGCTCTGATG ATTCTGCAGT TCAGATATGG 
AACTGGCAAT CAGAGGACCC TTTCTTTCTC CAAGCCCACC AGGAAACAGT GAAAGACTTC 
AGACTCTTGA AAAATTCAAG ACTTCTTTCC TGGTCATTTG ATGGAACAGT AAAGGTATGG 
AATATTATTA CCAGAAGAAT AGAAAAAGAT TTCGTGTGTC ACCAGGATAC AGTTCTTTCT 
TGTGATATTT CCTCTGATGC TACTAAGTTT TCTTCTACCT CTGCTGACAA GACTGCAAAG 
ATTTGGAGTT TTGAACTCCT TTCTCCTCTT CATGAATTGA GAGGCCACAA AGGCTGTGTG 
CGCTGCTCTT CCTTCTCTGT GAACAATACC TTGTTGGCGA CTGGAGACGA CAATGGAGAA 
ATCAGGATAT GGAATGTCTC AAACGGTGAG CTTCTTCATT TGTGTGCTCC GATTTCAGTA 
GAAGAAGGAG CTGCTACTCA TGGAGGCTGG GTGACCGACC TTTGCTTTTC TCCAGACACC 
AAAATGCTTG TTTCTGCTGG AGGATATCTC AAGTGGTGGA ATGTTGTCAC TGGGGAATCC 
TCACAGACCT TCTACACAAA CGGAACCAAT CTTAAGAAAA TACACGTGTC CCCTGACTTC 
AAAACATATG TGACTGTTGA TAATCTTGGT ATTTTATATA TTCTAAAGAT TTTAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010803500.1
CDS	78..3317
Translation	MSGIRDCFPGGVHWVSVGKQDKSGLLMKLQNLCARLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDIWDPWVLKAFDNQCQILLTTRDKSVTDSVMGPKYVVPVESGLGKEKGLEILSLFVNMKKADLPEQAHSIIQECKGSPLVVSLIGALLRDFPNRWEYYLRQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDLETEEVEDILQEFVNKSLLFCDRNGKSFLYYLHDLQVDFLAEKNRSQLQDLHKKMVTQFQRHHQLYSLSPDQEDCMYWYSFLAYHMASANMHKELCALMFSLDWIKAKTELVGPAHLIHEFVEYRHILDEKDGIDCENFQEFLSLNGHLLGRQPFPNIVQLGLCELETSEVYRQAKLQAKQEVDNGMLYLEWVNKKNMKNLSRLVVRPHTDAVYHACFSEDGQRIASCGADKTLQVFKAETGEKLLEIKAHEDEVLCCAFSADDKFIATCSVDKKVKIWNSMTGELVRIYDEHSEQVNCCHFTNNSNHLLLATGSNDYFLKLWDLNQEECRNTMFGHTNSVSHCRFSPDDQVLASCSADGTLKLWDVKSANERKSINVKQFFLNSEEPQEDLEVIVKCCSWSADGAKIMVAAKNKVFLFDIHSSGLLAEMHMGHHSAVQYCDFSPHNHLAVVALSQYCVELWSMDSGLKVADCRGHLSWVHCVMFSPDGSSFLTSSDDQTIRLWETKKVCKNSATVLKQEIDVVFQENEVTVLAVDNIRRLQLINGNTGQIDYLTEAQVSCCCLSPHLQYLAFGGEDGAIQILELLNNRIFQSRNGHKTTVRHIQFTADGKTLISSSDDSAVQIWNWQSEDPFFLQAHQETVKDFRLLKNSRLLSWSFDGTVKVWNIITRRIEKDFVCHQDTVLSCDISSDATKFSSTSADKTAKIWSFELLSPLHELRGHKGCVRCSSFSVNNTLLATGDDNGEIRIWNVSNGELLHLCAPISVEEGAATHGGWVTDLCFSPDTKMLVSAGGYLKWWNVVTGESSQTFYTNGTNLKKIHVSPDFKTYVTVDNLGILYILKILE

Target ORF information:

RefSeq Version	XM_010805198.2
Organism	Bos taurus(cattle)
Definition	Bos taurus apoptotic peptidase activating factor 1 (APAF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010805198.2

ORF Insert Sequence:

ATGAGTGGAA TACGTGATTG TTTCCCAGGA GGCGTGCATT GGGTGTCAGT TGGGAAACAG 
GACAAATCCG GGCTTCTGAT GAAACTACAG AACCTTTGTG CACGATTGGA TCAGGATGAG 
AGCTTCTCCC AGAGGCTTCC ACTTAATATT GAAGAGGCTA AAGACCGTCT CCGCATTCTG 
ATGCTGCGCA AACATCCAAG GTCTTTATTG ATCTTGGATG ATATTTGGGA TCCTTGGGTG 
TTAAAAGCTT TTGACAATCA GTGTCAAATT CTTCTCACAA CTAGAGATAA GAGTGTTACA 
GATTCAGTGA TGGGTCCTAA ATATGTAGTG CCTGTGGAGA GTGGTTTAGG AAAAGAAAAA 
GGACTTGAAA TTTTATCCCT TTTTGTTAAT ATGAAGAAAG CAGATTTGCC AGAACAAGCT 
CACAGTATTA TACAAGAATG TAAAGGTTCC CCTCTTGTAG TATCTTTAAT TGGTGCACTT 
TTACGAGATT TTCCCAACCG CTGGGAGTAC TACCTCAGGC AACTTCAGAA TAAGCAATTT 
AAGAGGATAA GAAAATCTTC ATCTTATGAC TATGAGGCTC TAGATGAAGC CATGTCTATA 
AGTGTTGAAA TGCTCAGAGA AGACATCAAA GATTATTACA CAGATCTTTC CATCTTACAG 
AAAGATGTTA AGGTGCCTAC AAAGGTGTTA TGTATCCTCT GGGACTTGGA AACGGAAGAA 
GTTGAAGACA TATTACAGGA ATTTGTTAAT AAGTCTCTCT TGTTCTGTGA CCGGAATGGA 
AAATCATTTC TTTATTATTT ACATGATCTG CAAGTAGATT TCCTTGCTGA AAAGAATCGC 
AGCCAGCTTC AGGATCTCCA CAAGAAGATG GTCACTCAGT TCCAGAGACA TCACCAGCTA 
TATTCTCTTT CACCAGATCA GGAGGATTGC ATGTATTGGT ACAGTTTTCT GGCCTATCAC 
ATGGCCAGTG CTAATATGCA CAAAGAACTG TGTGCTTTAA TGTTTTCCCT GGATTGGATT 
AAAGCAAAAA CGGAACTTGT AGGCCCTGCT CATCTGATTC ATGAATTTGT GGAATACAGG 
CATATATTGG ATGAAAAGGA TGGTATAGAC TGTGAGAATT TCCAGGAGTT TTTATCTTTA 
AATGGACATC TTCTTGGACG ACAGCCATTT CCTAATATTG TGCAACTGGG CCTCTGTGAA 
CTGGAAACTT CAGAGGTTTA TCGGCAAGCT AAGCTGCAGG CCAAGCAGGA GGTCGATAAC 
GGAATGCTTT ACCTGGAGTG GGTAAACAAA AAAAACATGA AGAACCTCTC CCGCTTGGTT 
GTCCGCCCCC ATACAGATGC TGTTTACCAT GCTTGCTTTT CTGAAGATGG TCAAAGAATA 
GCGTCTTGTG GAGCTGATAA AACTTTACAG GTATTCAAAG CTGAAACAGG AGAGAAACTT 
CTAGAAATCA AGGCTCATGA GGATGAAGTG CTTTGCTGTG CATTCTCTGC AGATGACAAA 
TTTATAGCAA CCTGCTCAGT GGATAAAAAA GTGAAGATTT GGAATTCAAT GACTGGGGAA 
CTAGTGCGTA TCTATGATGA GCACTCAGAG CAAGTCAATT GCTGCCACTT CACCAACAAT 
AGTAATCACC TTCTCTTAGC CACTGGTTCA AATGACTACT TCCTTAAACT CTGGGATCTG 
AATCAAGAAG AATGTCGAAA TACCATGTTT GGCCATACAA ATTCGGTCAG TCACTGCAGA 
TTTTCACCAG ATGATCAGGT TTTGGCCAGT TGTTCAGCTG ATGGAACATT AAAGCTTTGG 
GATGTGAAAT CTGCAAATGA GAGGAAAAGC ATTAATGTGA AGCAATTCTT CCTGAATTCA 
GAGGAGCCTC AGGAGGATCT GGAAGTGATA GTGAAATGCT GTTCGTGGTC TGCTGATGGT 
GCTAAGATAA TGGTGGCTGC GAAAAATAAA GTCTTTCTTT TTGACATTCA CTCCAGTGGC 
CTGTTGGCAG AAATGCACAT GGGCCATCAT AGCGCTGTCC AGTACTGTGA CTTCTCCCCC 
CACAACCATT TGGCAGTGGT GGCTTTGTCC CAGTACTGTG TGGAGTTGTG GAGTATGGAC 
TCAGGTTTAA AAGTAGCTGA TTGCAGAGGA CATTTAAGTT GGGTCCATTG TGTGATGTTT 
TCTCCTGATG GATCATCGTT TTTGACATCT TCTGATGACC AGACAATCAG GCTCTGGGAG 
ACAAAGAAAG TATGTAAGAA CTCTGCTACA GTGTTAAAGC AAGAAATAGA TGTTGTGTTT 
CAAGAAAATG AAGTGACGGT TCTTGCAGTT GACAATATAA GACGTCTGCA ACTCATTAAT 
GGAAACACAG GTCAGATTGA TTACCTAACT GAAGCTCAAG TGAGTTGCTG TTGTTTAAGT 
CCACATCTTC AGTACCTTGC ATTCGGAGGT GAAGATGGAG CCATTCAGAT TTTAGAACTC 
CTGAACAACA GAATCTTCCA ATCCAGAAAT GGGCACAAGA CCACTGTTCG GCACATCCAG 
TTCACAGCTG ATGGAAAGAC TCTTATCTCA AGCTCTGATG ATTCTGCAGT TCAGATATGG 
AACTGGCAAT CAGAGGACCC TTTCTTTCTC CAAGCCCACC AGGAAACAGT GAAAGACTTC 
AGACTCTTGA AAAATTCAAG ACTTCTTTCC TGGTCATTTG ATGGAACAGT AAAGGTATGG 
AATATTATTA CCAGAAGAAT AGAAAAAGAT TTCGTGTGTC ACCAGGATAC AGTTCTTTCT 
TGTGATATTT CCTCTGATGC TACTAAGTTT TCTTCTACCT CTGCTGACAA GACTGCAAAG 
ATTTGGAGTT TTGAACTCCT TTCTCCTCTT CATGAATTGA GAGGCCACAA AGGCTGTGTG 
CGCTGCTCTT CCTTCTCTGT GAACAATACC TTGTTGGCGA CTGGAGACGA CAATGGAGAA 
ATCAGGATAT GGAATGTCTC AAACGGTGAG CTTCTTCATT TGTGTGCTCC GATTTCAGTA 
GAAGAAGGAG CTGCTACTCA TGGAGGCTGG GTGACCGACC TTTGCTTTTC TCCAGACACC 
AAAATGCTTG TTTCTGCTGG AGGATATCTC AAGTGGTGGA ATGTTGTCAC TGGGGAATCC 
TCACAGACCT TCTACACAAA CGGAACCAAT CTTAAGAAAA TACACGTGTC CCCTGACTTC 
AAAACATATG TGACTGTTGA TAATCTTGGT ATTTTATATA TTCTAAAGAT TTTAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

Sequence evaluation of four pooled-tissue normalized bovine cDNA libraries and construction of a gene index for cattle.
Genome research11(4)626-30(2001 Apr)
Smith TP,Grosse WM,Freking BA,Roberts AJ,Stone RT,Casas E,Wray JE,White J,Cho J,Fahrenkrug SC,Bennett GL,Heaton MP,Laegreid WW,Rohrer GA,Chitko-McKown CG,Pertea G,Holt I,Karamycheva S,Liang F,Quackenbush J,Keele JW

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APAF1 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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