Apaf1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Apaf1
Entrez Gene ID	11783
Full Name	apoptotic peptidase activating factor 1
Synonyms	6230400I06Rik,Apaf-1,Apaf1l,fog,mKIAA0413
General protein information	Preferred Names apoptotic peptidase activating factor 1 Names apoptotic protease-activating factor 1 forebrain overgrowth
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 10 Map Location: 10 C2\|10 45.47 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_001042558.1	NP_001036023.1	apoptotic protease-activating factor 1 isoform 1
NM_001282947.1	NP_001269876.1	apoptotic protease-activating factor 1 isoform 2
NM_009684.2	NP_033814.2	apoptotic protease-activating factor 1 isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	APAF1	NP_863651.1
Canis lupus familiaris (dog)	APAF1	XP_003432082.1
Bos taurus (cattle)	APAF1	NP_001178436.1
Xenopus tropicalis (tropical clawed frog)	apaf1	NP_001106546.1
Gallus gallus (chicken)	APAF1	XP_416167.3
Danio rerio (zebrafish)	apaf1	NP_571683.1
Pan troglodytes (chimpanzee)	APAF1	XP_003313928.1
	APAF1	XP_001086945.1
Mus musculus (house mouse)	Apaf1	NP_033814.2
Rattus norvegicus (Norway rat)	Apaf1	NP_076469.1

Gene Ontology
Bibliography

Related articles in PubMed

A minimally sufficient model for rib proximal-distal patterning based on genetic analysis and agent-based simulations.
Fogel JL, Lakeland DL, Mah IK, Mariani FV
eLife6(2017 Oct)

microRNA-183 Mediates Protective Postconditioning of the Liver by Repressing Apaf-1.
Lin HC, Liu SY, Yen EY, Li TK, Lai IR
Antioxidants & redox signaling26(11)583-597(2017 Apr)

Syndecan 4 controls lymphatic vasculature remodeling during mouse embryonic development.
Wang Y, Baeyens N, Corti F, Tanaka K, Fang JS, Zhang J, Jin Y, Coon B, Hirschi KK, Schwartz MA, Simons M
Development (Cambridge, England)143(23)4441-4451(2016 12)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

miR-183 was induced in protective postconditioning and reduced reperfusion injury of the livers via the targeting of apoptotic signaling. miR-183 mediated the tolerance induced by iPoC in livers via Apaf-1 repressing.
Title: microRNA-183 Mediates Protective Postconditioning of the Liver by Repressing Apaf-1.

Apaf1 is required for proper cortical neuron differentiation since its deletion specifically impairs axonal outgrowth.
Title: Apaf1-deficient cortical neurons exhibit defects in axonal outgrowth.

Inhibition of Caspase-9 restricted, while Apaf-1 promoted, Chlamydia pneumoniae infection in HEp-2, HeLa, and mouse epithelial fibroblast (MEF) cells.
Title: An epistatic effect of apaf-1 and caspase-9 on chlamydial infection.

Apaf1's pivotal role during embryonic development as has been demonstrated to be in regulation of neuronal cell death
Title: Apaf1 in embryonic development - shaping life by death, and more.

results provide evidence that Apaf-1 pharmacological inhibition has therapeutic potential for the treatment of apoptosis-related diseases.
Title: Apaf-1 inhibitors protect from unwanted cell death in in vivo models of kidney ischemia and chemotherapy induced ototoxicity.

The following Apaf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Apaf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu16523	NM_009684.2 Latest version!	Mus musculus apoptotic peptidase activating factor 1 (Apaf1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu16523	NM_001042558.1 Latest version!	Mus musculus apoptotic peptidase activating factor 1 (Apaf1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OMu16834	NM_001282947.1 Latest version!	Mus musculus apoptotic peptidase activating factor 1 (Apaf1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Apaf1 ( NM_001042558.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001036023.1 Mus musculus apoptotic peptidase activating factor 1 (Apaf1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu16523
Clone ID Related Accession (Same CDS sequence)	NM_001042558.1 , NM_009684.2
Accession Version	NM_001042558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	apoptotic protease-activating factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ160169.1, AK220340.1, DT924066.1 and BC083191.1. Transcript Variant: This variant (1) has an additional segment in the 5' UTR, compared to variant 2. Variants 1 and 2 encode the same isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK220340.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGATGCAA AGGCCCGCAA TTGTTTGCTT CAACATAGAG AAGCTTTGGA AAAGGACATC 
AAAACATCCT ACATCATGGA TCACATGATC AGTAATGGCG TCTTGTCAGT GATAGAGGAG 
GAGAAGGTCA AAAGTCAGGC CACTCAATAT CAACGAGCAG CCGCTTTAAT TAAAATGATA 
CTTAATAAAG ACAACTGTGC CTACATTTCA TTCTACAACG CTCTGCTACA CGAGGGCTAT 
AAGGACCTTG CTGCGCTTCT GCAGAGTGGC CTTCCTCTTG TGTCGTCTTC CAGTGGCAAG 
GACACAGATG GTGGAATAAC TTCATTTGTA AGGACAGTGC TGTGTGAAGG TGGAGTACCC 
CAGAGGCCGG TTATTTTCGT TACTAGAAAG AAGCTGGTTC ATGCGATTCA GCAGAAGCTC 
TGGAAACTGA ATGGAGAACC AGGGTGGGTC ACCATCTATG GGATGGCAGG CTGCGGCAAG 
TCTGTGTTAG CTGCGGAAGC CGTTCGAGAT CACTCCCTCT TAGAAGGTTG CTTTTCAGGG 
GGTGTACACT GGGTTTCCAT TGGAAAACAA GACAAATCTG GGCTTCTCAT GAAACTGCAG 
AATCTGTGCA TGCGCTTGGA CCAAGAAGAG AGTTTCTCTC AGAGGCTTCC ACTTAATATT 
GAGGAGGCCA AAGACCGCCT CCGTGTTCTG ATGCTGCGCA AACACCCAAG GTCTCTGTTG 
ATCTTGGATG ATGTTTGGGA TCCTTGGGTG TTAAAAGCTT TTGACAATCA GTGTCAGATT 
CTTCTTACAA CCAGAGATAA GAGTGTTACA GATTCAGTAA TGGGTCCTAA GCATGTTGTC 
CCTGTGGAGA GTGGTCTAGG GAGAGAGAAA GGACTTGAGA TCTTGTCACT TTTTGTTAAC 
ATGAAGAAAG AAGATCTGCC AGCGGAGGCT CACAGTATTA TAAAGGAATG CAAAGGTTCT 
CCTCTTGTAG TGTCTTTAAT TGGTGCGCTT TTACGTGATT TTCCCAATCG CTGGGCATAC 
TACCTCAGAC AGCTTCAGAA TAAGCAGTTT AAGAGAATAA GGAAGTCTTC ATCTTATGAT 
TATGAGGCTC TAGATGAAGC CATGTCGATA AGTGTTGAAA TGCTCAGAGA AGACATCAAA 
GACTATTACA CAGACCTTTC CATCCTTCAG AAGGACGTCA AGGTACCTAC AAAGGTGTTG 
TGCGTTCTCT GGGACTTGGA AACGGAAGAA GTTGAAGACA TCCTGCAGGA GTTCGTTAAT 
AAGTCTCTCT TATTCTGTAA TCGGAATGGA AAGTCATTTT GTTATTATTT ACATGATCTT 
CAAGTAGATT TTCTTACAGA GAAGAATCGC AGTCAGCTTC AGGATCTGCA CAGGAAGATG 
GTCACTCAGT TTCAGAGGTA TTACCAGCCC CACACGCTGT CTCCAGACCA GGAGGACTGC 
ATGTATTGGT ACAACTTCCT AGCCTATCAC ATGGCTAGTG CCAATATGCA CAAAGAACTT 
TGTGCTTTAA TGTTTTCCCT GGACTGGATT AAAGCAAAAA CAGAACTTGT CGGCCCTGCC 
CATCTGATTC ACGAGTTCGT GGCATATAGG CATATATTGG ATGAAAAGGA TTGTGCAGTC 
TGTGAGAATT TTCAAGAGTT TTTATCTTTA AATGGACACC TCCTTGGACG ACAGCCATTT 
CCTAATATTG TACAGCTGGG CCTCTGTGAA CCAGAAACTT CCGAAGTTTA TCGACAAGCA 
AAGCTGCAGG CCAAGCAGGA GGGGGATACT GGGCGCCTTT ACCTGGAATG GATAAACAAA 
AAAACTATCA AGAATCTGTC CCGCTTAGTC GTCCGCCCCC ACACAGATGC TGTTTACCAC 
GCGTGTTTTT CTCAGGATGG TCAGAGAATA GCTTCTTGTG GGGCTGATAA AACCTTACAG 
GTGTTCAAAG CCGAGACAGG AGAGAAACTT CTTGACATCA AAGCTCACGA AGATGAGGTG 
CTTTGCTGCG CGTTCTCCTC AGACGACAGT TACATAGCGA CCTGCTCAGC GGATAAGAAG 
GTTAAGATTT GGGATTCTGC GACTGGGAAG CTTGTGCACA CCTACGACGA GCACTCGGAG 
CAAGTCAATT GCTGCCATTT CACCAACAAG AGTAACCACC TTCTCTTGGC CACTGGGTCA 
AATGATTTCT TCCTCAAGCT CTGGGATTTG AATCAAAAAG AATGTCGAAA TACCATGTTT 
GGTCACACGA ACTCAGTCAA CCACTGCAGG TTCTCACCAG ACGATGAGCT CTTGGCTAGC 
TGCTCAGCTG ACGGGACTTT AAGGCTTTGG GATGTGAGAT CAGCAAACGA GAGGAAAAGC 
ATTAATGTGA AGCGCTTCTT CCTGAGTTCA GAAGACCCTC CAGAGGATGT GGAGGTGATC 
GTGAAGTGTT GTTCCTGGTC TGCAGATGGT GACAAAATAA TAGTGGCAGC AAAAAACAAA 
GTCCTCCTTT TTGATATTCA TACTAGTGGC CTATTGGCAG AGATCCACAC AGGCCATCAC 
AGCACCATCC AGTACTGTGA CTTCTCCCCC TATGACCATT TGGCTGTGAT TGCCCTGTCT 
CAGTACTGTG TGGAGTTGTG GAACATAGAC TCCCGCCTAA AGGTGGCCGA CTGCAGAGGA 
CATTTGAGTT GGGTTCACGG TGTGATGTTT TCTCCCGATG GCTCCTCATT TTTGACAGCT 
TCTGATGACC AAACAATAAG GGTCTGGGAG ACAAAAAAGG TATGCAAGAA CTCTGCCATC 
GTGCTAAAGC AGGAAATAGA CGTCGTGTTT CAAGAGAACG AAACGATGGT CCTTGCAGTT 
GACAACATAA GAGGCCTGCA ACTCATTGCT GGAAAAACAG GCCAGATTGA TTACCTGCCT 
GAAGCCCAAG TGAGTTGCTG CTGCCTCAGT CCACACCTTG AGTACGTGGC ATTCGGAGAT 
GAAGATGGAG CCATTAAGAT TATAGAACTT CCAAACAACA GAGTCTTCAG TTCTGGGGTT 
GGGCACAAGA AAGCTGTGCG GCACATCCAG TTCACAGCTG ATGGGAAGAC ACTGATTTCA 
AGTTCTGAAG ATTCTGTGAT TCAGGTATGG AATTGGCAGA CAGGGGACTA TGTATTTTTG 
CAAGCCCACC AGGAAACGGT AAAGGACTTC AGGCTCCTCC AAGATTCAAG ATTGCTTTCT 
TGGTCATTTG ATGGAACGGT GAAGGTGTGG AATGTCATTA CCGGAAGAAT AGAAAGAGAC 
TTTACTTGTC ATCAGGGCAC AGTGCTTTCC TGTGCTATCT CTTCTGATGC GACCAAGTTT 
TCATCTACCT CTGCTGATAA GACTGCCAAG ATCTGGAGTT TTGACCTCCT TTCCCCTCTT 
CATGAGCTGA AGGGCCATAA TGGCTGTGTC CGCTGCTCTG CCTTCTCGCT GGATGGCATC 
CTGTTGGCAA CTGGAGATGA CAATGGAGAA ATCCGGATAT GGAATGTCTC AGATGGCCAG 
CTTCTTCATT CGTGTGCTCC GATCTCGGTA GAGGAAGGAA CTGCTACCCA CGGCGGCTGG 
GTAACTGATG TGTGCTTCTC TCCCGACAGT AAAACGCTTG TCTCTGCTGG AGGATATCTC 
AAGTGGTGGA ATGTTGCCAC TGGGGACTCC TCACAGACCT TCTACACAAA TGGAACAAAC 
CTCAAGAAAA TCCACGTGTC CCCTGACTTC AGAACCTATG TGACTGTCGA TAATCTCGGT 
ATTTTATATA TTTTACAGGT TTTAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001036023.1
CDS	669..4418
Translation	MDAKARNCLLQHREALEKDIKTSYIMDHMISNGVLSVIEEEKVKSQATQYQRAAALIKMILNKDNCAYISFYNALLHEGYKDLAALLQSGLPLVSSSSGKDTDGGITSFVRTVLCEGGVPQRPVIFVTRKKLVHAIQQKLWKLNGEPGWVTIYGMAGCGKSVLAAEAVRDHSLLEGCFSGGVHWVSIGKQDKSGLLMKLQNLCMRLDQEESFSQRLPLNIEEAKDRLRVLMLRKHPRSLLILDDVWDPWVLKAFDNQCQILLTTRDKSVTDSVMGPKHVVPVESGLGREKGLEILSLFVNMKKEDLPAEAHSIIKECKGSPLVVSLIGALLRDFPNRWAYYLRQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCVLWDLETEEVEDILQEFVNKSLLFCNRNGKSFCYYLHDLQVDFLTEKNRSQLQDLHRKMVTQFQRYYQPHTLSPDQEDCMYWYNFLAYHMASANMHKELCALMFSLDWIKAKTELVGPAHLIHEFVAYRHILDEKDCAVCENFQEFLSLNGHLLGRQPFPNIVQLGLCEPETSEVYRQAKLQAKQEGDTGRLYLEWINKKTIKNLSRLVVRPHTDAVYHACFSQDGQRIASCGADKTLQVFKAETGEKLLDIKAHEDEVLCCAFSSDDSYIATCSADKKVKIWDSATGKLVHTYDEHSEQVNCCHFTNKSNHLLLATGSNDFFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDELLASCSADGTLRLWDVRSANERKSINVKRFFLSSEDPPEDVEVIVKCCSWSADGDKIIVAAKNKVLLFDIHTSGLLAEIHTGHHSTIQYCDFSPYDHLAVIALSQYCVELWNIDSRLKVADCRGHLSWVHGVMFSPDGSSFLTASDDQTIRVWETKKVCKNSAIVLKQEIDVVFQENETMVLAVDNIRGLQLIAGKTGQIDYLPEAQVSCCCLSPHLEYVAFGDEDGAIKIIELPNNRVFSSGVGHKKAVRHIQFTADGKTLISSSEDSVIQVWNWQTGDYVFLQAHQETVKDFRLLQDSRLLSWSFDGTVKVWNVITGRIERDFTCHQGTVLSCAISSDATKFSSTSADKTAKIWSFDLLSPLHELKGHNGCVRCSAFSLDGILLATGDDNGEIRIWNVSDGQLLHSCAPISVEEGTATHGGWVTDVCFSPDSKTLVSAGGYLKWWNVATGDSSQTFYTNGTNLKKIHVSPDFRTYVTVDNLGILYILQVLE

Target ORF information:

RefSeq Version	NM_001042558.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus apoptotic peptidase activating factor 1 (Apaf1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001042558.1

ORF Insert Sequence:

ATGGATGCAA AGGCCCGCAA TTGTTTGCTT CAACATAGAG AAGCTTTGGA AAAGGACATC 
AAAACATCCT ACATCATGGA TCACATGATC AGTAATGGCG TCTTGTCAGT GATAGAGGAG 
GAGAAGGTCA AAAGTCAGGC CACTCAATAT CAACGAGCAG CCGCTTTAAT TAAAATGATA 
CTTAATAAAG ACAACTGTGC CTACATTTCA TTCTACAACG CTCTGCTACA CGAGGGCTAT 
AAGGACCTTG CTGCGCTTCT GCAGAGTGGC CTTCCTCTTG TGTCGTCTTC CAGTGGCAAG 
GACACAGATG GTGGAATAAC TTCATTTGTA AGGACAGTGC TGTGTGAAGG TGGAGTACCC 
CAGAGGCCGG TTATTTTCGT TACTAGAAAG AAGCTGGTTC ATGCGATTCA GCAGAAGCTC 
TGGAAACTGA ATGGAGAACC AGGGTGGGTC ACCATCTATG GGATGGCAGG CTGCGGCAAG 
TCTGTGTTAG CTGCGGAAGC CGTTCGAGAT CACTCCCTCT TAGAAGGTTG CTTTTCAGGG 
GGTGTACACT GGGTTTCCAT TGGAAAACAA GACAAATCTG GGCTTCTCAT GAAACTGCAG 
AATCTGTGCA TGCGCTTGGA CCAAGAAGAG AGTTTCTCTC AGAGGCTTCC ACTTAATATT 
GAGGAGGCCA AAGACCGCCT CCGTGTTCTG ATGCTGCGCA AACACCCAAG GTCTCTGTTG 
ATCTTGGATG ATGTTTGGGA TCCTTGGGTG TTAAAAGCTT TTGACAATCA GTGTCAGATT 
CTTCTTACAA CCAGAGATAA GAGTGTTACA GATTCAGTAA TGGGTCCTAA GCATGTTGTC 
CCTGTGGAGA GTGGTCTAGG GAGAGAGAAA GGACTTGAGA TCTTGTCACT TTTTGTTAAC 
ATGAAGAAAG AAGATCTGCC AGCGGAGGCT CACAGTATTA TAAAGGAATG CAAAGGTTCT 
CCTCTTGTAG TGTCTTTAAT TGGTGCGCTT TTACGTGATT TTCCCAATCG CTGGGCATAC 
TACCTCAGAC AGCTTCAGAA TAAGCAGTTT AAGAGAATAA GGAAGTCTTC ATCTTATGAT 
TATGAGGCTC TAGATGAAGC CATGTCGATA AGTGTTGAAA TGCTCAGAGA AGACATCAAA 
GACTATTACA CAGACCTTTC CATCCTTCAG AAGGACGTCA AGGTACCTAC AAAGGTGTTG 
TGCGTTCTCT GGGACTTGGA AACGGAAGAA GTTGAAGACA TCCTGCAGGA GTTCGTTAAT 
AAGTCTCTCT TATTCTGTAA TCGGAATGGA AAGTCATTTT GTTATTATTT ACATGATCTT 
CAAGTAGATT TTCTTACAGA GAAGAATCGC AGTCAGCTTC AGGATCTGCA CAGGAAGATG 
GTCACTCAGT TTCAGAGGTA TTACCAGCCC CACACGCTGT CTCCAGACCA GGAGGACTGC 
ATGTATTGGT ACAACTTCCT AGCCTATCAC ATGGCTAGTG CCAATATGCA CAAAGAACTT 
TGTGCTTTAA TGTTTTCCCT GGACTGGATT AAAGCAAAAA CAGAACTTGT CGGCCCTGCC 
CATCTGATTC ACGAGTTCGT GGCATATAGG CATATATTGG ATGAAAAGGA TTGTGCAGTC 
TGTGAGAATT TTCAAGAGTT TTTATCTTTA AATGGACACC TCCTTGGACG ACAGCCATTT 
CCTAATATTG TACAGCTGGG CCTCTGTGAA CCAGAAACTT CCGAAGTTTA TCGACAAGCA 
AAGCTGCAGG CCAAGCAGGA GGGGGATACT GGGCGCCTTT ACCTGGAATG GATAAACAAA 
AAAACTATCA AGAATCTGTC CCGCTTAGTC GTCCGCCCCC ACACAGATGC TGTTTACCAC 
GCGTGTTTTT CTCAGGATGG TCAGAGAATA GCTTCTTGTG GGGCTGATAA AACCTTACAG 
GTGTTCAAAG CCGAGACAGG AGAGAAACTT CTTGACATCA AAGCTCACGA AGATGAGGTG 
CTTTGCTGCG CGTTCTCCTC AGACGACAGT TACATAGCGA CCTGCTCAGC GGATAAGAAG 
GTTAAGATTT GGGATTCTGC GACTGGGAAG CTTGTGCACA CCTACGACGA GCACTCGGAG 
CAAGTCAATT GCTGCCATTT CACCAACAAG AGTAACCACC TTCTCTTGGC CACTGGGTCA 
AATGATTTCT TCCTCAAGCT CTGGGATTTG AATCAAAAAG AATGTCGAAA TACCATGTTT 
GGTCACACGA ACTCAGTCAA CCACTGCAGG TTCTCACCAG ACGATGAGCT CTTGGCTAGC 
TGCTCAGCTG ACGGGACTTT AAGGCTTTGG GATGTGAGAT CAGCAAACGA GAGGAAAAGC 
ATTAATGTGA AGCGCTTCTT CCTGAGTTCA GAAGACCCTC CAGAGGATGT GGAGGTGATC 
GTGAAGTGTT GTTCCTGGTC TGCAGATGGT GACAAAATAA TAGTGGCAGC AAAAAACAAA 
GTCCTCCTTT TTGATATTCA TACTAGTGGC CTATTGGCAG AGATCCACAC AGGCCATCAC 
AGCACCATCC AGTACTGTGA CTTCTCCCCC TATGACCATT TGGCTGTGAT TGCCCTGTCT 
CAGTACTGTG TGGAGTTGTG GAACATAGAC TCCCGCCTAA AGGTGGCCGA CTGCAGAGGA 
CATTTGAGTT GGGTTCACGG TGTGATGTTT TCTCCCGATG GCTCCTCATT TTTGACAGCT 
TCTGATGACC AAACAATAAG GGTCTGGGAG ACAAAAAAGG TATGCAAGAA CTCTGCCATC 
GTGCTAAAGC AGGAAATAGA CGTCGTGTTT CAAGAGAACG AAACGATGGT CCTTGCAGTT 
GACAACATAA GAGGCCTGCA ACTCATTGCT GGAAAAACAG GCCAGATTGA TTACCTGCCT 
GAAGCCCAAG TGAGTTGCTG CTGCCTCAGT CCACACCTTG AGTACGTGGC ATTCGGAGAT 
GAAGATGGAG CCATTAAGAT TATAGAACTT CCAAACAACA GAGTCTTCAG TTCTGGGGTT 
GGGCACAAGA AAGCTGTGCG GCACATCCAG TTCACAGCTG ATGGGAAGAC ACTGATTTCA 
AGTTCTGAAG ATTCTGTGAT TCAGGTATGG AATTGGCAGA CAGGGGACTA TGTATTTTTG 
CAAGCCCACC AGGAAACGGT AAAGGACTTC AGGCTCCTCC AAGATTCAAG ATTGCTTTCT 
TGGTCATTTG ATGGAACGGT GAAGGTGTGG AATGTCATTA CCGGAAGAAT AGAAAGAGAC 
TTTACTTGTC ATCAGGGCAC AGTGCTTTCC TGTGCTATCT CTTCTGATGC GACCAAGTTT 
TCATCTACCT CTGCTGATAA GACTGCCAAG ATCTGGAGTT TTGACCTCCT TTCCCCTCTT 
CATGAGCTGA AGGGCCATAA TGGCTGTGTC CGCTGCTCTG CCTTCTCGCT GGATGGCATC 
CTGTTGGCAA CTGGAGATGA CAATGGAGAA ATCCGGATAT GGAATGTCTC AGATGGCCAG 
CTTCTTCATT CGTGTGCTCC GATCTCGGTA GAGGAAGGAA CTGCTACCCA CGGCGGCTGG 
GTAACTGATG TGTGCTTCTC TCCCGACAGT AAAACGCTTG TCTCTGCTGG AGGATATCTC 
AAGTGGTGGA ATGTTGCCAC TGGGGACTCC TCACAGACCT TCTACACAAA TGGAACAAAC 
CTCAAGAAAA TCCACGTGTC CCCTGACTTC AGAACCTATG TGACTGTCGA TAATCTCGGT 
ATTTTATATA TTTTACAGGT TTTAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apaf1 ( NM_009684.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033814.2 Mus musculus apoptotic peptidase activating factor 1 (Apaf1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu16523
Clone ID Related Accession (Same CDS sequence)	NM_001042558.1 , NM_009684.2
Accession Version	NM_009684.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	apoptotic protease-activating factor 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CJ160169.1, AK220340.1, DT924066.1 and BC083191.1. On Jul 14, 2006 this sequence version replaced NM_009684.1. Transcript Variant: This variant (2) encodes the longer isoform (1). Variants 1 and 2 encode the same isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC083191.1, BC131683.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGATGCAA AGGCCCGCAA TTGTTTGCTT CAACATAGAG AAGCTTTGGA AAAGGACATC 
AAAACATCCT ACATCATGGA TCACATGATC AGTAATGGCG TCTTGTCAGT GATAGAGGAG 
GAGAAGGTCA AAAGTCAGGC CACTCAATAT CAACGAGCAG CCGCTTTAAT TAAAATGATA 
CTTAATAAAG ACAACTGTGC CTACATTTCA TTCTACAACG CTCTGCTACA CGAGGGCTAT 
AAGGACCTTG CTGCGCTTCT GCAGAGTGGC CTTCCTCTTG TGTCGTCTTC CAGTGGCAAG 
GACACAGATG GTGGAATAAC TTCATTTGTA AGGACAGTGC TGTGTGAAGG TGGAGTACCC 
CAGAGGCCGG TTATTTTCGT TACTAGAAAG AAGCTGGTTC ATGCGATTCA GCAGAAGCTC 
TGGAAACTGA ATGGAGAACC AGGGTGGGTC ACCATCTATG GGATGGCAGG CTGCGGCAAG 
TCTGTGTTAG CTGCGGAAGC CGTTCGAGAT CACTCCCTCT TAGAAGGTTG CTTTTCAGGG 
GGTGTACACT GGGTTTCCAT TGGAAAACAA GACAAATCTG GGCTTCTCAT GAAACTGCAG 
AATCTGTGCA TGCGCTTGGA CCAAGAAGAG AGTTTCTCTC AGAGGCTTCC ACTTAATATT 
GAGGAGGCCA AAGACCGCCT CCGTGTTCTG ATGCTGCGCA AACACCCAAG GTCTCTGTTG 
ATCTTGGATG ATGTTTGGGA TCCTTGGGTG TTAAAAGCTT TTGACAATCA GTGTCAGATT 
CTTCTTACAA CCAGAGATAA GAGTGTTACA GATTCAGTAA TGGGTCCTAA GCATGTTGTC 
CCTGTGGAGA GTGGTCTAGG GAGAGAGAAA GGACTTGAGA TCTTGTCACT TTTTGTTAAC 
ATGAAGAAAG AAGATCTGCC AGCGGAGGCT CACAGTATTA TAAAGGAATG CAAAGGTTCT 
CCTCTTGTAG TGTCTTTAAT TGGTGCGCTT TTACGTGATT TTCCCAATCG CTGGGCATAC 
TACCTCAGAC AGCTTCAGAA TAAGCAGTTT AAGAGAATAA GGAAGTCTTC ATCTTATGAT 
TATGAGGCTC TAGATGAAGC CATGTCGATA AGTGTTGAAA TGCTCAGAGA AGACATCAAA 
GACTATTACA CAGACCTTTC CATCCTTCAG AAGGACGTCA AGGTACCTAC AAAGGTGTTG 
TGCGTTCTCT GGGACTTGGA AACGGAAGAA GTTGAAGACA TCCTGCAGGA GTTCGTTAAT 
AAGTCTCTCT TATTCTGTAA TCGGAATGGA AAGTCATTTT GTTATTATTT ACATGATCTT 
CAAGTAGATT TTCTTACAGA GAAGAATCGC AGTCAGCTTC AGGATCTGCA CAGGAAGATG 
GTCACTCAGT TTCAGAGGTA TTACCAGCCC CACACGCTGT CTCCAGACCA GGAGGACTGC 
ATGTATTGGT ACAACTTCCT AGCCTATCAC ATGGCTAGTG CCAATATGCA CAAAGAACTT 
TGTGCTTTAA TGTTTTCCCT GGACTGGATT AAAGCAAAAA CAGAACTTGT CGGCCCTGCC 
CATCTGATTC ACGAGTTCGT GGCATATAGG CATATATTGG ATGAAAAGGA TTGTGCAGTC 
TGTGAGAATT TTCAAGAGTT TTTATCTTTA AATGGACACC TCCTTGGACG ACAGCCATTT 
CCTAATATTG TACAGCTGGG CCTCTGTGAA CCAGAAACTT CCGAAGTTTA TCGACAAGCA 
AAGCTGCAGG CCAAGCAGGA GGGGGATACT GGGCGCCTTT ACCTGGAATG GATAAACAAA 
AAAACTATCA AGAATCTGTC CCGCTTAGTC GTCCGCCCCC ACACAGATGC TGTTTACCAC 
GCGTGTTTTT CTCAGGATGG TCAGAGAATA GCTTCTTGTG GGGCTGATAA AACCTTACAG 
GTGTTCAAAG CCGAGACAGG AGAGAAACTT CTTGACATCA AAGCTCACGA AGATGAGGTG 
CTTTGCTGCG CGTTCTCCTC AGACGACAGT TACATAGCGA CCTGCTCAGC GGATAAGAAG 
GTTAAGATTT GGGATTCTGC GACTGGGAAG CTTGTGCACA CCTACGACGA GCACTCGGAG 
CAAGTCAATT GCTGCCATTT CACCAACAAG AGTAACCACC TTCTCTTGGC CACTGGGTCA 
AATGATTTCT TCCTCAAGCT CTGGGATTTG AATCAAAAAG AATGTCGAAA TACCATGTTT 
GGTCACACGA ACTCAGTCAA CCACTGCAGG TTCTCACCAG ACGATGAGCT CTTGGCTAGC 
TGCTCAGCTG ACGGGACTTT AAGGCTTTGG GATGTGAGAT CAGCAAACGA GAGGAAAAGC 
ATTAATGTGA AGCGCTTCTT CCTGAGTTCA GAAGACCCTC CAGAGGATGT GGAGGTGATC 
GTGAAGTGTT GTTCCTGGTC TGCAGATGGT GACAAAATAA TAGTGGCAGC AAAAAACAAA 
GTCCTCCTTT TTGATATTCA TACTAGTGGC CTATTGGCAG AGATCCACAC AGGCCATCAC 
AGCACCATCC AGTACTGTGA CTTCTCCCCC TATGACCATT TGGCTGTGAT TGCCCTGTCT 
CAGTACTGTG TGGAGTTGTG GAACATAGAC TCCCGCCTAA AGGTGGCCGA CTGCAGAGGA 
CATTTGAGTT GGGTTCACGG TGTGATGTTT TCTCCCGATG GCTCCTCATT TTTGACAGCT 
TCTGATGACC AAACAATAAG GGTCTGGGAG ACAAAAAAGG TATGCAAGAA CTCTGCCATC 
GTGCTAAAGC AGGAAATAGA CGTCGTGTTT CAAGAGAACG AAACGATGGT CCTTGCAGTT 
GACAACATAA GAGGCCTGCA ACTCATTGCT GGAAAAACAG GCCAGATTGA TTACCTGCCT 
GAAGCCCAAG TGAGTTGCTG CTGCCTCAGT CCACACCTTG AGTACGTGGC ATTCGGAGAT 
GAAGATGGAG CCATTAAGAT TATAGAACTT CCAAACAACA GAGTCTTCAG TTCTGGGGTT 
GGGCACAAGA AAGCTGTGCG GCACATCCAG TTCACAGCTG ATGGGAAGAC ACTGATTTCA 
AGTTCTGAAG ATTCTGTGAT TCAGGTATGG AATTGGCAGA CAGGGGACTA TGTATTTTTG 
CAAGCCCACC AGGAAACGGT AAAGGACTTC AGGCTCCTCC AAGATTCAAG ATTGCTTTCT 
TGGTCATTTG ATGGAACGGT GAAGGTGTGG AATGTCATTA CCGGAAGAAT AGAAAGAGAC 
TTTACTTGTC ATCAGGGCAC AGTGCTTTCC TGTGCTATCT CTTCTGATGC GACCAAGTTT 
TCATCTACCT CTGCTGATAA GACTGCCAAG ATCTGGAGTT TTGACCTCCT TTCCCCTCTT 
CATGAGCTGA AGGGCCATAA TGGCTGTGTC CGCTGCTCTG CCTTCTCGCT GGATGGCATC 
CTGTTGGCAA CTGGAGATGA CAATGGAGAA ATCCGGATAT GGAATGTCTC AGATGGCCAG 
CTTCTTCATT CGTGTGCTCC GATCTCGGTA GAGGAAGGAA CTGCTACCCA CGGCGGCTGG 
GTAACTGATG TGTGCTTCTC TCCCGACAGT AAAACGCTTG TCTCTGCTGG AGGATATCTC 
AAGTGGTGGA ATGTTGCCAC TGGGGACTCC TCACAGACCT TCTACACAAA TGGAACAAAC 
CTCAAGAAAA TCCACGTGTC CCCTGACTTC AGAACCTATG TGACTGTCGA TAATCTCGGT 
ATTTTATATA TTTTACAGGT TTTAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033814.2
CDS	626..4375
Translation	MDAKARNCLLQHREALEKDIKTSYIMDHMISNGVLSVIEEEKVKSQATQYQRAAALIKMILNKDNCAYISFYNALLHEGYKDLAALLQSGLPLVSSSSGKDTDGGITSFVRTVLCEGGVPQRPVIFVTRKKLVHAIQQKLWKLNGEPGWVTIYGMAGCGKSVLAAEAVRDHSLLEGCFSGGVHWVSIGKQDKSGLLMKLQNLCMRLDQEESFSQRLPLNIEEAKDRLRVLMLRKHPRSLLILDDVWDPWVLKAFDNQCQILLTTRDKSVTDSVMGPKHVVPVESGLGREKGLEILSLFVNMKKEDLPAEAHSIIKECKGSPLVVSLIGALLRDFPNRWAYYLRQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCVLWDLETEEVEDILQEFVNKSLLFCNRNGKSFCYYLHDLQVDFLTEKNRSQLQDLHRKMVTQFQRYYQPHTLSPDQEDCMYWYNFLAYHMASANMHKELCALMFSLDWIKAKTELVGPAHLIHEFVAYRHILDEKDCAVCENFQEFLSLNGHLLGRQPFPNIVQLGLCEPETSEVYRQAKLQAKQEGDTGRLYLEWINKKTIKNLSRLVVRPHTDAVYHACFSQDGQRIASCGADKTLQVFKAETGEKLLDIKAHEDEVLCCAFSSDDSYIATCSADKKVKIWDSATGKLVHTYDEHSEQVNCCHFTNKSNHLLLATGSNDFFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDELLASCSADGTLRLWDVRSANERKSINVKRFFLSSEDPPEDVEVIVKCCSWSADGDKIIVAAKNKVLLFDIHTSGLLAEIHTGHHSTIQYCDFSPYDHLAVIALSQYCVELWNIDSRLKVADCRGHLSWVHGVMFSPDGSSFLTASDDQTIRVWETKKVCKNSAIVLKQEIDVVFQENETMVLAVDNIRGLQLIAGKTGQIDYLPEAQVSCCCLSPHLEYVAFGDEDGAIKIIELPNNRVFSSGVGHKKAVRHIQFTADGKTLISSSEDSVIQVWNWQTGDYVFLQAHQETVKDFRLLQDSRLLSWSFDGTVKVWNVITGRIERDFTCHQGTVLSCAISSDATKFSSTSADKTAKIWSFDLLSPLHELKGHNGCVRCSAFSLDGILLATGDDNGEIRIWNVSDGQLLHSCAPISVEEGTATHGGWVTDVCFSPDSKTLVSAGGYLKWWNVATGDSSQTFYTNGTNLKKIHVSPDFRTYVTVDNLGILYILQVLE

Target ORF information:

RefSeq Version	NM_009684.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus apoptotic peptidase activating factor 1 (Apaf1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009684.2

ORF Insert Sequence:

ATGGATGCAA AGGCCCGCAA TTGTTTGCTT CAACATAGAG AAGCTTTGGA AAAGGACATC 
AAAACATCCT ACATCATGGA TCACATGATC AGTAATGGCG TCTTGTCAGT GATAGAGGAG 
GAGAAGGTCA AAAGTCAGGC CACTCAATAT CAACGAGCAG CCGCTTTAAT TAAAATGATA 
CTTAATAAAG ACAACTGTGC CTACATTTCA TTCTACAACG CTCTGCTACA CGAGGGCTAT 
AAGGACCTTG CTGCGCTTCT GCAGAGTGGC CTTCCTCTTG TGTCGTCTTC CAGTGGCAAG 
GACACAGATG GTGGAATAAC TTCATTTGTA AGGACAGTGC TGTGTGAAGG TGGAGTACCC 
CAGAGGCCGG TTATTTTCGT TACTAGAAAG AAGCTGGTTC ATGCGATTCA GCAGAAGCTC 
TGGAAACTGA ATGGAGAACC AGGGTGGGTC ACCATCTATG GGATGGCAGG CTGCGGCAAG 
TCTGTGTTAG CTGCGGAAGC CGTTCGAGAT CACTCCCTCT TAGAAGGTTG CTTTTCAGGG 
GGTGTACACT GGGTTTCCAT TGGAAAACAA GACAAATCTG GGCTTCTCAT GAAACTGCAG 
AATCTGTGCA TGCGCTTGGA CCAAGAAGAG AGTTTCTCTC AGAGGCTTCC ACTTAATATT 
GAGGAGGCCA AAGACCGCCT CCGTGTTCTG ATGCTGCGCA AACACCCAAG GTCTCTGTTG 
ATCTTGGATG ATGTTTGGGA TCCTTGGGTG TTAAAAGCTT TTGACAATCA GTGTCAGATT 
CTTCTTACAA CCAGAGATAA GAGTGTTACA GATTCAGTAA TGGGTCCTAA GCATGTTGTC 
CCTGTGGAGA GTGGTCTAGG GAGAGAGAAA GGACTTGAGA TCTTGTCACT TTTTGTTAAC 
ATGAAGAAAG AAGATCTGCC AGCGGAGGCT CACAGTATTA TAAAGGAATG CAAAGGTTCT 
CCTCTTGTAG TGTCTTTAAT TGGTGCGCTT TTACGTGATT TTCCCAATCG CTGGGCATAC 
TACCTCAGAC AGCTTCAGAA TAAGCAGTTT AAGAGAATAA GGAAGTCTTC ATCTTATGAT 
TATGAGGCTC TAGATGAAGC CATGTCGATA AGTGTTGAAA TGCTCAGAGA AGACATCAAA 
GACTATTACA CAGACCTTTC CATCCTTCAG AAGGACGTCA AGGTACCTAC AAAGGTGTTG 
TGCGTTCTCT GGGACTTGGA AACGGAAGAA GTTGAAGACA TCCTGCAGGA GTTCGTTAAT 
AAGTCTCTCT TATTCTGTAA TCGGAATGGA AAGTCATTTT GTTATTATTT ACATGATCTT 
CAAGTAGATT TTCTTACAGA GAAGAATCGC AGTCAGCTTC AGGATCTGCA CAGGAAGATG 
GTCACTCAGT TTCAGAGGTA TTACCAGCCC CACACGCTGT CTCCAGACCA GGAGGACTGC 
ATGTATTGGT ACAACTTCCT AGCCTATCAC ATGGCTAGTG CCAATATGCA CAAAGAACTT 
TGTGCTTTAA TGTTTTCCCT GGACTGGATT AAAGCAAAAA CAGAACTTGT CGGCCCTGCC 
CATCTGATTC ACGAGTTCGT GGCATATAGG CATATATTGG ATGAAAAGGA TTGTGCAGTC 
TGTGAGAATT TTCAAGAGTT TTTATCTTTA AATGGACACC TCCTTGGACG ACAGCCATTT 
CCTAATATTG TACAGCTGGG CCTCTGTGAA CCAGAAACTT CCGAAGTTTA TCGACAAGCA 
AAGCTGCAGG CCAAGCAGGA GGGGGATACT GGGCGCCTTT ACCTGGAATG GATAAACAAA 
AAAACTATCA AGAATCTGTC CCGCTTAGTC GTCCGCCCCC ACACAGATGC TGTTTACCAC 
GCGTGTTTTT CTCAGGATGG TCAGAGAATA GCTTCTTGTG GGGCTGATAA AACCTTACAG 
GTGTTCAAAG CCGAGACAGG AGAGAAACTT CTTGACATCA AAGCTCACGA AGATGAGGTG 
CTTTGCTGCG CGTTCTCCTC AGACGACAGT TACATAGCGA CCTGCTCAGC GGATAAGAAG 
GTTAAGATTT GGGATTCTGC GACTGGGAAG CTTGTGCACA CCTACGACGA GCACTCGGAG 
CAAGTCAATT GCTGCCATTT CACCAACAAG AGTAACCACC TTCTCTTGGC CACTGGGTCA 
AATGATTTCT TCCTCAAGCT CTGGGATTTG AATCAAAAAG AATGTCGAAA TACCATGTTT 
GGTCACACGA ACTCAGTCAA CCACTGCAGG TTCTCACCAG ACGATGAGCT CTTGGCTAGC 
TGCTCAGCTG ACGGGACTTT AAGGCTTTGG GATGTGAGAT CAGCAAACGA GAGGAAAAGC 
ATTAATGTGA AGCGCTTCTT CCTGAGTTCA GAAGACCCTC CAGAGGATGT GGAGGTGATC 
GTGAAGTGTT GTTCCTGGTC TGCAGATGGT GACAAAATAA TAGTGGCAGC AAAAAACAAA 
GTCCTCCTTT TTGATATTCA TACTAGTGGC CTATTGGCAG AGATCCACAC AGGCCATCAC 
AGCACCATCC AGTACTGTGA CTTCTCCCCC TATGACCATT TGGCTGTGAT TGCCCTGTCT 
CAGTACTGTG TGGAGTTGTG GAACATAGAC TCCCGCCTAA AGGTGGCCGA CTGCAGAGGA 
CATTTGAGTT GGGTTCACGG TGTGATGTTT TCTCCCGATG GCTCCTCATT TTTGACAGCT 
TCTGATGACC AAACAATAAG GGTCTGGGAG ACAAAAAAGG TATGCAAGAA CTCTGCCATC 
GTGCTAAAGC AGGAAATAGA CGTCGTGTTT CAAGAGAACG AAACGATGGT CCTTGCAGTT 
GACAACATAA GAGGCCTGCA ACTCATTGCT GGAAAAACAG GCCAGATTGA TTACCTGCCT 
GAAGCCCAAG TGAGTTGCTG CTGCCTCAGT CCACACCTTG AGTACGTGGC ATTCGGAGAT 
GAAGATGGAG CCATTAAGAT TATAGAACTT CCAAACAACA GAGTCTTCAG TTCTGGGGTT 
GGGCACAAGA AAGCTGTGCG GCACATCCAG TTCACAGCTG ATGGGAAGAC ACTGATTTCA 
AGTTCTGAAG ATTCTGTGAT TCAGGTATGG AATTGGCAGA CAGGGGACTA TGTATTTTTG 
CAAGCCCACC AGGAAACGGT AAAGGACTTC AGGCTCCTCC AAGATTCAAG ATTGCTTTCT 
TGGTCATTTG ATGGAACGGT GAAGGTGTGG AATGTCATTA CCGGAAGAAT AGAAAGAGAC 
TTTACTTGTC ATCAGGGCAC AGTGCTTTCC TGTGCTATCT CTTCTGATGC GACCAAGTTT 
TCATCTACCT CTGCTGATAA GACTGCCAAG ATCTGGAGTT TTGACCTCCT TTCCCCTCTT 
CATGAGCTGA AGGGCCATAA TGGCTGTGTC CGCTGCTCTG CCTTCTCGCT GGATGGCATC 
CTGTTGGCAA CTGGAGATGA CAATGGAGAA ATCCGGATAT GGAATGTCTC AGATGGCCAG 
CTTCTTCATT CGTGTGCTCC GATCTCGGTA GAGGAAGGAA CTGCTACCCA CGGCGGCTGG 
GTAACTGATG TGTGCTTCTC TCCCGACAGT AAAACGCTTG TCTCTGCTGG AGGATATCTC 
AAGTGGTGGA ATGTTGCCAC TGGGGACTCC TCACAGACCT TCTACACAAA TGGAACAAAC 
CTCAAGAAAA TCCACGTGTC CCCTGACTTC AGAACCTATG TGACTGTCGA TAATCTCGGT 
ATTTTATATA TTTTACAGGT TTTAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Apaf1 ( NM_001282947.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001269876.1 Mus musculus apoptotic peptidase activating factor 1 (Apaf1), transcript variant 3, mRNA.

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CloneID	OMu16834
Clone ID Related Accession (Same CDS sequence)	NM_001282947.1
Accession Version	NM_001282947.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-20
Organism	Mus musculus(house mouse)
Product	apoptotic protease-activating factor 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BY293164.1, AK220340.1, CF539822.1 and BC083191.1. Transcript Variant: This variant (3) lacks an in-frame segment in the 5' coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF064071.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END##

ATGGATGCAA AGGCCCGCAA TTGTTTGCTT CAACATAGAG AAGCTTTGGA AAAGGACATC 
AAAACATCCT ACATCATGGA TCACATGATC AGTAATGGCG TCTTGTCAGT GATAGAGGAG 
GAGAAGGTCA AAAGTCAGGC CACTCAATAT CAACGAGCAG CCGCTTTAAT TAAAATGATA 
CTTAATAAAG ACAACTGTGC CTACATTTCA TTCTACAACG CTCTGCTACA CGAGGGCTAT 
AAGGACCTTG CTGCGCTTCT GCAGAGTGGC CTTCCTCTTG TGTCGTCTTC CAGTGTAAGG 
ACAGTGCTGT GTGAAGGTGG AGTACCCCAG AGGCCGGTTA TTTTCGTTAC TAGAAAGAAG 
CTGGTTCATG CGATTCAGCA GAAGCTCTGG AAACTGAATG GAGAACCAGG GTGGGTCACC 
ATCTATGGGA TGGCAGGCTG CGGCAAGTCT GTGTTAGCTG CGGAAGCCGT TCGAGATCAC 
TCCCTCTTAG AAGGTTGCTT TTCAGGGGGT GTACACTGGG TTTCCATTGG AAAACAAGAC 
AAATCTGGGC TTCTCATGAA ACTGCAGAAT CTGTGCATGC GCTTGGACCA AGAAGAGAGT 
TTCTCTCAGA GGCTTCCACT TAATATTGAG GAGGCCAAAG ACCGCCTCCG TGTTCTGATG 
CTGCGCAAAC ACCCAAGGTC TCTGTTGATC TTGGATGATG TTTGGGATCC TTGGGTGTTA 
AAAGCTTTTG ACAATCAGTG TCAGATTCTT CTTACAACCA GAGATAAGAG TGTTACAGAT 
TCAGTAATGG GTCCTAAGCA TGTTGTCCCT GTGGAGAGTG GTCTAGGGAG AGAGAAAGGA 
CTTGAGATCT TGTCACTTTT TGTTAACATG AAGAAAGAAG ATCTGCCAGC GGAGGCTCAC 
AGTATTATAA AGGAATGCAA AGGTTCTCCT CTTGTAGTGT CTTTAATTGG TGCGCTTTTA 
CGTGATTTTC CCAATCGCTG GGCATACTAC CTCAGACAGC TTCAGAATAA GCAGTTTAAG 
AGAATAAGGA AGTCTTCATC TTATGATTAT GAGGCTCTAG ATGAAGCCAT GTCGATAAGT 
GTTGAAATGC TCAGAGAAGA CATCAAAGAC TATTACACAG ACCTTTCCAT CCTTCAGAAG 
GACGTCAAGG TACCTACAAA GGTGTTGTGC GTTCTCTGGG ACTTGGAAAC GGAAGAAGTT 
GAAGACATCC TGCAGGAGTT CGTTAATAAG TCTCTCTTAT TCTGTAATCG GAATGGAAAG 
TCATTTTGTT ATTATTTACA TGATCTTCAA GTAGATTTTC TTACAGAGAA GAATCGCAGT 
CAGCTTCAGG ATCTGCACAG GAAGATGGTC ACTCAGTTTC AGAGGTATTA CCAGCCCCAC 
ACGCTGTCTC CAGACCAGGA GGACTGCATG TATTGGTACA ACTTCCTAGC CTATCACATG 
GCTAGTGCCA ATATGCACAA AGAACTTTGT GCTTTAATGT TTTCCCTGGA CTGGATTAAA 
GCAAAAACAG AACTTGTCGG CCCTGCCCAT CTGATTCACG AGTTCGTGGC ATATAGGCAT 
ATATTGGATG AAAAGGATTG TGCAGTCTGT GAGAATTTTC AAGAGTTTTT ATCTTTAAAT 
GGACACCTCC TTGGACGACA GCCATTTCCT AATATTGTAC AGCTGGGCCT CTGTGAACCA 
GAAACTTCCG AAGTTTATCG ACAAGCAAAG CTGCAGGCCA AGCAGGAGGG GGATACTGGG 
CGCCTTTACC TGGAATGGAT AAACAAAAAA ACTATCAAGA ATCTGTCCCG CTTAGTCGTC 
CGCCCCCACA CAGATGCTGT TTACCACGCG TGTTTTTCTC AGGATGGTCA GAGAATAGCT 
TCTTGTGGGG CTGATAAAAC CTTACAGGTG TTCAAAGCCG AGACAGGAGA GAAACTTCTT 
GACATCAAAG CTCACGAAGA TGAGGTGCTT TGCTGCGCGT TCTCCTCAGA CGACAGTTAC 
ATAGCGACCT GCTCAGCGGA TAAGAAGGTT AAGATTTGGG ATTCTGCGAC TGGGAAGCTT 
GTGCACACCT ACGACGAGCA CTCGGAGCAA GTCAATTGCT GCCATTTCAC CAACAAGAGT 
AACCACCTTC TCTTGGCCAC TGGGTCAAAT GATTTCTTCC TCAAGCTCTG GGATTTGAAT 
CAAAAAGAAT GTCGAAATAC CATGTTTGGT CACACGAACT CAGTCAACCA CTGCAGGTTC 
TCACCAGACG ATGAGCTCTT GGCTAGCTGC TCAGCTGACG GGACTTTAAG GCTTTGGGAT 
GTGAGATCAG CAAACGAGAG GAAAAGCATT AATGTGAAGC GCTTCTTCCT GAGTTCAGAA 
GACCCTCCAG AGGATGTGGA GGTGATCGTG AAGTGTTGTT CCTGGTCTGC AGATGGTGAC 
AAAATAATAG TGGCAGCAAA AAACAAAGTC CTCCTTTTTG ATATTCATAC TAGTGGCCTA 
TTGGCAGAGA TCCACACAGG CCATCACAGC ACCATCCAGT ACTGTGACTT CTCCCCCTAT 
GACCATTTGG CTGTGATTGC CCTGTCTCAG TACTGTGTGG AGTTGTGGAA CATAGACTCC 
CGCCTAAAGG TGGCCGACTG CAGAGGACAT TTGAGTTGGG TTCACGGTGT GATGTTTTCT 
CCCGATGGCT CCTCATTTTT GACAGCTTCT GATGACCAAA CAATAAGGGT CTGGGAGACA 
AAAAAGGTAT GCAAGAACTC TGCCATCGTG CTAAAGCAGG AAATAGACGT CGTGTTTCAA 
GAGAACGAAA CGATGGTCCT TGCAGTTGAC AACATAAGAG GCCTGCAACT CATTGCTGGA 
AAAACAGGCC AGATTGATTA CCTGCCTGAA GCCCAAGTGA GTTGCTGCTG CCTCAGTCCA 
CACCTTGAGT ACGTGGCATT CGGAGATGAA GATGGAGCCA TTAAGATTAT AGAACTTCCA 
AACAACAGAG TCTTCAGTTC TGGGGTTGGG CACAAGAAAG CTGTGCGGCA CATCCAGTTC 
ACAGCTGATG GGAAGACACT GATTTCAAGT TCTGAAGATT CTGTGATTCA GGTATGGAAT 
TGGCAGACAG GGGACTATGT ATTTTTGCAA GCCCACCAGG AAACGGTAAA GGACTTCAGG 
CTCCTCCAAG ATTCAAGATT GCTTTCTTGG TCATTTGATG GAACGGTGAA GGTGTGGAAT 
GTCATTACCG GAAGAATAGA AAGAGACTTT ACTTGTCATC AGGGCACAGT GCTTTCCTGT 
GCTATCTCTT CTGATGCGAC CAAGTTTTCA TCTACCTCTG CTGATAAGAC TGCCAAGATC 
TGGAGTTTTG ACCTCCTTTC CCCTCTTCAT GAGCTGAAGG GCCATAATGG CTGTGTCCGC 
TGCTCTGCCT TCTCGCTGGA TGGCATCCTG TTGGCAACTG GAGATGACAA TGGAGAAATC 
CGGATATGGA ATGTCTCAGA TGGCCAGCTT CTTCATTCGT GTGCTCCGAT CTCGGTAGAG 
GAAGGAACTG CTACCCACGG CGGCTGGGTA ACTGATGTGT GCTTCTCTCC CGACAGTAAA 
ACGCTTGTCT CTGCTGGAGG ATATCTCAAG TGGTGGAATG TTGCCACTGG GGACTCCTCA 
CAGACCTTCT ACACAAATGG AACAAACCTC AAGAAAATCC ACGTGTCCCC TGACTTCAGA 
ACCTATGTGA CTGTCGATAA TCTCGGTATT TTATATATTT TACAGGTTTT AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001269876.1
CDS	626..4342
Translation	MDAKARNCLLQHREALEKDIKTSYIMDHMISNGVLSVIEEEKVKSQATQYQRAAALIKMILNKDNCAYISFYNALLHEGYKDLAALLQSGLPLVSSSSVRTVLCEGGVPQRPVIFVTRKKLVHAIQQKLWKLNGEPGWVTIYGMAGCGKSVLAAEAVRDHSLLEGCFSGGVHWVSIGKQDKSGLLMKLQNLCMRLDQEESFSQRLPLNIEEAKDRLRVLMLRKHPRSLLILDDVWDPWVLKAFDNQCQILLTTRDKSVTDSVMGPKHVVPVESGLGREKGLEILSLFVNMKKEDLPAEAHSIIKECKGSPLVVSLIGALLRDFPNRWAYYLRQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCVLWDLETEEVEDILQEFVNKSLLFCNRNGKSFCYYLHDLQVDFLTEKNRSQLQDLHRKMVTQFQRYYQPHTLSPDQEDCMYWYNFLAYHMASANMHKELCALMFSLDWIKAKTELVGPAHLIHEFVAYRHILDEKDCAVCENFQEFLSLNGHLLGRQPFPNIVQLGLCEPETSEVYRQAKLQAKQEGDTGRLYLEWINKKTIKNLSRLVVRPHTDAVYHACFSQDGQRIASCGADKTLQVFKAETGEKLLDIKAHEDEVLCCAFSSDDSYIATCSADKKVKIWDSATGKLVHTYDEHSEQVNCCHFTNKSNHLLLATGSNDFFLKLWDLNQKECRNTMFGHTNSVNHCRFSPDDELLASCSADGTLRLWDVRSANERKSINVKRFFLSSEDPPEDVEVIVKCCSWSADGDKIIVAAKNKVLLFDIHTSGLLAEIHTGHHSTIQYCDFSPYDHLAVIALSQYCVELWNIDSRLKVADCRGHLSWVHGVMFSPDGSSFLTASDDQTIRVWETKKVCKNSAIVLKQEIDVVFQENETMVLAVDNIRGLQLIAGKTGQIDYLPEAQVSCCCLSPHLEYVAFGDEDGAIKIIELPNNRVFSSGVGHKKAVRHIQFTADGKTLISSSEDSVIQVWNWQTGDYVFLQAHQETVKDFRLLQDSRLLSWSFDGTVKVWNVITGRIERDFTCHQGTVLSCAISSDATKFSSTSADKTAKIWSFDLLSPLHELKGHNGCVRCSAFSLDGILLATGDDNGEIRIWNVSDGQLLHSCAPISVEEGTATHGGWVTDVCFSPDSKTLVSAGGYLKWWNVATGDSSQTFYTNGTNLKKIHVSPDFRTYVTVDNLGILYILQVLE

Target ORF information:

RefSeq Version	NM_001282947.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus apoptotic peptidase activating factor 1 (Apaf1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001282947.1

ORF Insert Sequence:

ATGGATGCAA AGGCCCGCAA TTGTTTGCTT CAACATAGAG AAGCTTTGGA AAAGGACATC 
AAAACATCCT ACATCATGGA TCACATGATC AGTAATGGCG TCTTGTCAGT GATAGAGGAG 
GAGAAGGTCA AAAGTCAGGC CACTCAATAT CAACGAGCAG CCGCTTTAAT TAAAATGATA 
CTTAATAAAG ACAACTGTGC CTACATTTCA TTCTACAACG CTCTGCTACA CGAGGGCTAT 
AAGGACCTTG CTGCGCTTCT GCAGAGTGGC CTTCCTCTTG TGTCGTCTTC CAGTGTAAGG 
ACAGTGCTGT GTGAAGGTGG AGTACCCCAG AGGCCGGTTA TTTTCGTTAC TAGAAAGAAG 
CTGGTTCATG CGATTCAGCA GAAGCTCTGG AAACTGAATG GAGAACCAGG GTGGGTCACC 
ATCTATGGGA TGGCAGGCTG CGGCAAGTCT GTGTTAGCTG CGGAAGCCGT TCGAGATCAC 
TCCCTCTTAG AAGGTTGCTT TTCAGGGGGT GTACACTGGG TTTCCATTGG AAAACAAGAC 
AAATCTGGGC TTCTCATGAA ACTGCAGAAT CTGTGCATGC GCTTGGACCA AGAAGAGAGT 
TTCTCTCAGA GGCTTCCACT TAATATTGAG GAGGCCAAAG ACCGCCTCCG TGTTCTGATG 
CTGCGCAAAC ACCCAAGGTC TCTGTTGATC TTGGATGATG TTTGGGATCC TTGGGTGTTA 
AAAGCTTTTG ACAATCAGTG TCAGATTCTT CTTACAACCA GAGATAAGAG TGTTACAGAT 
TCAGTAATGG GTCCTAAGCA TGTTGTCCCT GTGGAGAGTG GTCTAGGGAG AGAGAAAGGA 
CTTGAGATCT TGTCACTTTT TGTTAACATG AAGAAAGAAG ATCTGCCAGC GGAGGCTCAC 
AGTATTATAA AGGAATGCAA AGGTTCTCCT CTTGTAGTGT CTTTAATTGG TGCGCTTTTA 
CGTGATTTTC CCAATCGCTG GGCATACTAC CTCAGACAGC TTCAGAATAA GCAGTTTAAG 
AGAATAAGGA AGTCTTCATC TTATGATTAT GAGGCTCTAG ATGAAGCCAT GTCGATAAGT 
GTTGAAATGC TCAGAGAAGA CATCAAAGAC TATTACACAG ACCTTTCCAT CCTTCAGAAG 
GACGTCAAGG TACCTACAAA GGTGTTGTGC GTTCTCTGGG ACTTGGAAAC GGAAGAAGTT 
GAAGACATCC TGCAGGAGTT CGTTAATAAG TCTCTCTTAT TCTGTAATCG GAATGGAAAG 
TCATTTTGTT ATTATTTACA TGATCTTCAA GTAGATTTTC TTACAGAGAA GAATCGCAGT 
CAGCTTCAGG ATCTGCACAG GAAGATGGTC ACTCAGTTTC AGAGGTATTA CCAGCCCCAC 
ACGCTGTCTC CAGACCAGGA GGACTGCATG TATTGGTACA ACTTCCTAGC CTATCACATG 
GCTAGTGCCA ATATGCACAA AGAACTTTGT GCTTTAATGT TTTCCCTGGA CTGGATTAAA 
GCAAAAACAG AACTTGTCGG CCCTGCCCAT CTGATTCACG AGTTCGTGGC ATATAGGCAT 
ATATTGGATG AAAAGGATTG TGCAGTCTGT GAGAATTTTC AAGAGTTTTT ATCTTTAAAT 
GGACACCTCC TTGGACGACA GCCATTTCCT AATATTGTAC AGCTGGGCCT CTGTGAACCA 
GAAACTTCCG AAGTTTATCG ACAAGCAAAG CTGCAGGCCA AGCAGGAGGG GGATACTGGG 
CGCCTTTACC TGGAATGGAT AAACAAAAAA ACTATCAAGA ATCTGTCCCG CTTAGTCGTC 
CGCCCCCACA CAGATGCTGT TTACCACGCG TGTTTTTCTC AGGATGGTCA GAGAATAGCT 
TCTTGTGGGG CTGATAAAAC CTTACAGGTG TTCAAAGCCG AGACAGGAGA GAAACTTCTT 
GACATCAAAG CTCACGAAGA TGAGGTGCTT TGCTGCGCGT TCTCCTCAGA CGACAGTTAC 
ATAGCGACCT GCTCAGCGGA TAAGAAGGTT AAGATTTGGG ATTCTGCGAC TGGGAAGCTT 
GTGCACACCT ACGACGAGCA CTCGGAGCAA GTCAATTGCT GCCATTTCAC CAACAAGAGT 
AACCACCTTC TCTTGGCCAC TGGGTCAAAT GATTTCTTCC TCAAGCTCTG GGATTTGAAT 
CAAAAAGAAT GTCGAAATAC CATGTTTGGT CACACGAACT CAGTCAACCA CTGCAGGTTC 
TCACCAGACG ATGAGCTCTT GGCTAGCTGC TCAGCTGACG GGACTTTAAG GCTTTGGGAT 
GTGAGATCAG CAAACGAGAG GAAAAGCATT AATGTGAAGC GCTTCTTCCT GAGTTCAGAA 
GACCCTCCAG AGGATGTGGA GGTGATCGTG AAGTGTTGTT CCTGGTCTGC AGATGGTGAC 
AAAATAATAG TGGCAGCAAA AAACAAAGTC CTCCTTTTTG ATATTCATAC TAGTGGCCTA 
TTGGCAGAGA TCCACACAGG CCATCACAGC ACCATCCAGT ACTGTGACTT CTCCCCCTAT 
GACCATTTGG CTGTGATTGC CCTGTCTCAG TACTGTGTGG AGTTGTGGAA CATAGACTCC 
CGCCTAAAGG TGGCCGACTG CAGAGGACAT TTGAGTTGGG TTCACGGTGT GATGTTTTCT 
CCCGATGGCT CCTCATTTTT GACAGCTTCT GATGACCAAA CAATAAGGGT CTGGGAGACA 
AAAAAGGTAT GCAAGAACTC TGCCATCGTG CTAAAGCAGG AAATAGACGT CGTGTTTCAA 
GAGAACGAAA CGATGGTCCT TGCAGTTGAC AACATAAGAG GCCTGCAACT CATTGCTGGA 
AAAACAGGCC AGATTGATTA CCTGCCTGAA GCCCAAGTGA GTTGCTGCTG CCTCAGTCCA 
CACCTTGAGT ACGTGGCATT CGGAGATGAA GATGGAGCCA TTAAGATTAT AGAACTTCCA 
AACAACAGAG TCTTCAGTTC TGGGGTTGGG CACAAGAAAG CTGTGCGGCA CATCCAGTTC 
ACAGCTGATG GGAAGACACT GATTTCAAGT TCTGAAGATT CTGTGATTCA GGTATGGAAT 
TGGCAGACAG GGGACTATGT ATTTTTGCAA GCCCACCAGG AAACGGTAAA GGACTTCAGG 
CTCCTCCAAG ATTCAAGATT GCTTTCTTGG TCATTTGATG GAACGGTGAA GGTGTGGAAT 
GTCATTACCG GAAGAATAGA AAGAGACTTT ACTTGTCATC AGGGCACAGT GCTTTCCTGT 
GCTATCTCTT CTGATGCGAC CAAGTTTTCA TCTACCTCTG CTGATAAGAC TGCCAAGATC 
TGGAGTTTTG ACCTCCTTTC CCCTCTTCAT GAGCTGAAGG GCCATAATGG CTGTGTCCGC 
TGCTCTGCCT TCTCGCTGGA TGGCATCCTG TTGGCAACTG GAGATGACAA TGGAGAAATC 
CGGATATGGA ATGTCTCAGA TGGCCAGCTT CTTCATTCGT GTGCTCCGAT CTCGGTAGAG 
GAAGGAACTG CTACCCACGG CGGCTGGGTA ACTGATGTGT GCTTCTCTCC CGACAGTAAA 
ACGCTTGTCT CTGCTGGAGG ATATCTCAAG TGGTGGAATG TTGCCACTGG GGACTCCTCA 
CAGACCTTCT ACACAAATGG AACAAACCTC AAGAAAATCC ACGTGTCCCC TGACTTCAGA 
ACCTATGTGA CTGTCGATAA TCTCGGTATT TTATATATTT TACAGGTTTT AGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A minimally sufficient model for rib proximal-distal patterning based on genetic analysis and agent-based simulations.
eLife6(2017 Oct)
Fogel JL,Lakeland DL,Mah IK,Mariani FV

microRNA-183 Mediates Protective Postconditioning of the Liver by Repressing Apaf-1.
Antioxidants & redox signaling26(11)583-597(2017 Apr)
Lin HC,Liu SY,Yen EY,Li TK,Lai IR

Syndecan 4 controls lymphatic vasculature remodeling during mouse embryonic development.
Development (Cambridge, England)143(23)4441-4451(2016 12)
Wang Y,Baeyens N,Corti F,Tanaka K,Fang JS,Zhang J,Jin Y,Coon B,Hirschi KK,Schwartz MA,Simons M

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Apaf1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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