ORF » Species Summary » Homo sapiens » APAF1 cDNA ORF clone

APAF1 cDNA ORF clone, Homo sapiens(human)

  • Gene

  • Clones

  • gRNAs

The following APAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu21167 NM_181869.1
Latest version!		 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $202.30
$289.00
OHu83749 XM_017019250.1
Latest version!		 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OHu20629 NM_181861.2
Latest version!		 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $881.30
$1259.00
OHu20835 NM_013229.3
Latest version!		 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $874.30
$1249.00
OHu21003 NM_181868.1
Latest version!		 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $853.30
$1219.00
OHu21771 NM_001160.3
Latest version!		 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $839.30
$1199.00
View Old Accession Versions >>
OHu20835 NM_013229.2 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $874.30
$1249.00
OHu20629 NM_181861.1 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $881.30
$1259.00
OHu21771 NM_001160.2 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $839.30
$1199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    APAF1 ( NM_181869.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_863659.1 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 5, mRNA.
    Price & Availability↑
    CloneID OHu21167
    Clone ID Related Accession (Same CDS sequence) NM_181869.1
    Accession Version NM_181869.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1017bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product apoptotic protease-activating factor 1 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF013263.1, AB103079.1 and AC011248.8. Transcript Variant: This variant (5), also known as APAF-1-ALT, lacks several exons in the coding sequence, resulting in a frameshift and an early termination codon compared to variant 3. Variant 5 encodes isoform e, which is shorter and has a distinct C-terminus compared to isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB103079.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGGTAAA 
    GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
    CAGAGGCCAG TTGTTTTTGT CACAAGGAAG AAGCTGGTGA ATGCAATTCA GCAGAAGCTC 
    TCCAAATTGA AAGGTGAACC AGGATGGGTC ACCATACATG GAATGGCAGG CTGTGGGAAG 
    TCTGTATTAG CTGCAGAAGC TGTTAGAGAT CATTCCCTTT TAGAAGGTTG TTTCCCAGGG 
    GGAGTGCATT GGGTTTCAGT TGGGAAACAA GACAAATCTG GGCTTCTGAT GAAACTGCAG 
    AATCTTTGCA CACGGTTGGA TCAGGATGAG AGTTTTTCCC AGAGGCTTCC ACTTAATATT 
    GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTTCGCA AACACCCAAG GTCTCTCTTG 
    ATCTTGGATG ATGTTTGGGA CTCTTGGGTG TTGAAAGCTT TTGACAGTCA GTGTCAGATT 
    CTTCTTACAA CCAGAGACAA GAGTGTTACA GATTCAGTAA TGGGTCCTAA ATATGTAGTC 
    CCTGTGGAGA GTTCCTTAGG AAAGGAAAAA GGACTTGAAA TTTTATCCCT TTTTGTTAAT 
    ATGAAGAAGG CAGATTTGCC AGAACAAGCT CATAGTATTA TAAAAGAATG TAAAGTGGTG 
    GAACGTTGTC ACTGGGGAAT CCTCACAGAC CTTCTACACA AATGGAACCA ATCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_863659.1
    CDS578..1594
    Translation

    Target ORF information:

    RefSeq Version NM_181869.1
    Organism Homo sapiens(human)
    Definition Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_181869.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGGTAAA 
    GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
    CAGAGGCCAG TTGTTTTTGT CACAAGGAAG AAGCTGGTGA ATGCAATTCA GCAGAAGCTC 
    TCCAAATTGA AAGGTGAACC AGGATGGGTC ACCATACATG GAATGGCAGG CTGTGGGAAG 
    TCTGTATTAG CTGCAGAAGC TGTTAGAGAT CATTCCCTTT TAGAAGGTTG TTTCCCAGGG 
    GGAGTGCATT GGGTTTCAGT TGGGAAACAA GACAAATCTG GGCTTCTGAT GAAACTGCAG 
    AATCTTTGCA CACGGTTGGA TCAGGATGAG AGTTTTTCCC AGAGGCTTCC ACTTAATATT 
    GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTTCGCA AACACCCAAG GTCTCTCTTG 
    ATCTTGGATG ATGTTTGGGA CTCTTGGGTG TTGAAAGCTT TTGACAGTCA GTGTCAGATT 
    CTTCTTACAA CCAGAGACAA GAGTGTTACA GATTCAGTAA TGGGTCCTAA ATATGTAGTC 
    CCTGTGGAGA GTTCCTTAGG AAAGGAAAAA GGACTTGAAA TTTTATCCCT TTTTGTTAAT 
    ATGAAGAAGG CAGATTTGCC AGAACAAGCT CATAGTATTA TAAAAGAATG TAAAGTGGTG 
    GAACGTTGTC ACTGGGGAAT CCTCACAGAC CTTCTACACA AATGGAACCA ATCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    APAF1 ( XM_017019250.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016874739.1 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant X2, mRNA.
    Price & Availability↑
    CloneID OHu83749
    Clone ID Related Accession (Same CDS sequence) XM_017019250.1
    Accession Version XM_017019250.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product apoptotic protease-activating factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGGTAAA 
    GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
    CAGAGGCCAG TTGTTTTTGT CACAAGGAAG AAGCTGGTGA ATGCAATTCA GCAGAAGCTC 
    TCCAAATTGA AAGGTGAACC AGGATGGGTC ACCATACATG GAATGGCAGG CTGTGGGAAG 
    TCTGTATTAG CTGCAGAAGC TGTTAGAGAT CATTCCCTTT TAGAAGGTTG TTTCCCAGGG 
    GGAGTGCATT GGGTTTCAGT TGGGAAACAA GACAAATCTG GGCTTCTGAT GAAACTGCAG 
    AATCTTTGCA CACGGTTGGA TCAGGATGAG AGTTTTTCCC AGAGGCTTCC ACTTAATATT 
    GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTTCGCA AACACCCAAG GTCTCTCTTG 
    ATCTTGGATG ATGTTTGGGA CTCTTGGGTG TTGAAAGCTT TTGACAGTCA GTGTCAGATT 
    CTTCTTACAA CCAGAGACAA GAGTGTTACA GATTCAGTAA TGGGTCCTAA ATATGTAGTC 
    CCTGTGGAGA GTTCCTTAGG AAAGGAAAAA GGACTTGAAA TTTTATCCCT TTTTGTTAAT 
    ATGAAGAAGG CAGATTTGCC AGAACAAGCT CATAGTATTA TAAAAGAATG TAAAGACAGA 
    GTCTCACACT GTTCCTCAGG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016874739.1
    CDS737..1720
    Translation

    Target ORF information:

    RefSeq Version XM_017019250.1
    Organism Homo sapiens(human)
    Definition Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_017019250.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGGTAAA 
    GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
    CAGAGGCCAG TTGTTTTTGT CACAAGGAAG AAGCTGGTGA ATGCAATTCA GCAGAAGCTC 
    TCCAAATTGA AAGGTGAACC AGGATGGGTC ACCATACATG GAATGGCAGG CTGTGGGAAG 
    TCTGTATTAG CTGCAGAAGC TGTTAGAGAT CATTCCCTTT TAGAAGGTTG TTTCCCAGGG 
    GGAGTGCATT GGGTTTCAGT TGGGAAACAA GACAAATCTG GGCTTCTGAT GAAACTGCAG 
    AATCTTTGCA CACGGTTGGA TCAGGATGAG AGTTTTTCCC AGAGGCTTCC ACTTAATATT 
    GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTTCGCA AACACCCAAG GTCTCTCTTG 
    ATCTTGGATG ATGTTTGGGA CTCTTGGGTG TTGAAAGCTT TTGACAGTCA GTGTCAGATT 
    CTTCTTACAA CCAGAGACAA GAGTGTTACA GATTCAGTAA TGGGTCCTAA ATATGTAGTC 
    CCTGTGGAGA GTTCCTTAGG AAAGGAAAAA GGACTTGAAA TTTTATCCCT TTTTGTTAAT 
    ATGAAGAAGG CAGATTTGCC AGAACAAGCT CATAGTATTA TAAAAGAATG TAAAGACAGA 
    GTCTCACACT GTTCCTCAGG CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    APAF1 ( NM_181861.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_863651.1 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu20629
    Clone ID Related Accession (Same CDS sequence) NM_181861.1 , NM_181861.2
    Accession Version NM_181861.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-18
    Organism Homo sapiens(human)
    Product apoptotic protease-activating factor 1 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF013263.1, AF149794.1 and AC011248.8. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3), also known as Apaf-1L, encodes the longest isoform (c) of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.187595.1, SRR1803614.51709.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGGTAAA 
    GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
    CAGAGGCCAG TTGTTTTTGT CACAAGGAAG AAGCTGGTGA ATGCAATTCA GCAGAAGCTC 
    TCCAAATTGA AAGGTGAACC AGGATGGGTC ACCATACATG GAATGGCAGG CTGTGGGAAG 
    TCTGTATTAG CTGCAGAAGC TGTTAGAGAT CATTCCCTTT TAGAAGGTTG TTTCCCAGGG 
    GGAGTGCATT GGGTTTCAGT TGGGAAACAA GACAAATCTG GGCTTCTGAT GAAACTGCAG 
    AATCTTTGCA CACGGTTGGA TCAGGATGAG AGTTTTTCCC AGAGGCTTCC ACTTAATATT 
    GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTTCGCA AACACCCAAG GTCTCTCTTG 
    ATCTTGGATG ATGTTTGGGA CTCTTGGGTG TTGAAAGCTT TTGACAGTCA GTGTCAGATT 
    CTTCTTACAA CCAGAGACAA GAGTGTTACA GATTCAGTAA TGGGTCCTAA ATATGTAGTC 
    CCTGTGGAGA GTTCCTTAGG AAAGGAAAAA GGACTTGAAA TTTTATCCCT TTTTGTTAAT 
    ATGAAGAAGG CAGATTTGCC AGAACAAGCT CATAGTATTA TAAAAGAATG TAAAGGCTCT 
    CCCCTTGTAG TATCTTTAAT TGGTGCACTT TTACGTGATT TTCCCAATCG CTGGGAGTAC 
    TACCTCAAAC AGCTTCAGAA TAAGCAGTTT AAGAGAATAA GGAAATCTTC GTCTTATGAT 
    TATGAGGCTC TAGATGAAGC CATGTCTATA AGTGTTGAAA TGCTCAGAGA AGACATCAAA 
    GATTATTACA CAGATCTTTC CATCCTTCAG AAGGACGTTA AGGTGCCTAC AAAGGTGTTA 
    TGTATTCTCT GGGACATGGA AACTGAAGAA GTTGAAGACA TACTGCAGGA GTTTGTAAAT 
    AAGTCTCTTT TATTCTGTGA TCGGAATGGA AAGTCGTTTC GTTATTATTT ACATGATCTT 
    CAAGTAGATT TTCTTACAGA GAAGAATTGC AGCCAGCTTC AGGATCTACA TAAGAAGATA 
    ATCACTCAGT TTCAGAGATA TCACCAGCCG CATACTCTTT CACCAGATCA GGAAGACTGT 
    ATGTATTGGT ACAACTTTCT GGCCTATCAC ATGGCCAGTG CCAAGATGCA CAAGGAACTT 
    TGTGCTTTAA TGTTTTCCCT GGATTGGATT AAAGCAAAAA CAGAACTTGT AGGCCCTGCT 
    CATCTGATTC ATGAATTTGT GGAATACAGA CATATACTAG ATGAAAAGGA TTGTGCAGTC 
    AGTGAGAATT TTCAGGAGTT TTTATCTTTA AATGGACACC TTCTTGGACG ACAGCCATTT 
    CCTAATATTG TACAACTGGG TCTCTGTGAG CCGGAAACTT CAGAAGTTTA TCAGCAAGCT 
    AAGCTGCAGG CCAAGCAGGA GGTCGATAAT GGAATGCTTT ACCTGGAATG GATAAACAAA 
    AAAAACATCA CGAATCTTTC CCGCTTAGTT GTCCGCCCCC ACACAGATGC TGTTTACCAT 
    GCCTGCTTTT CTGAGGATGG TCAGAGAATA GCTTCTTGTG GAGCTGATAA AACCTTACAG 
    GTGTTCAAAG CTGAAACAGG AGAGAAACTT CTAGAAATCA AGGCTCATGA GGATGAAGTG 
    CTTTGTTGTG CATTCTCTAC AGATGACAGA TTTATAGCAA CCTGCTCAGT GGATAAAAAA 
    GTGAAGATTT GGAATTCTAT GACTGGGGAA CTAGTACACA CCTATGATGA GCACTCAGAG 
    CAAGTCAATT GCTGCCATTT CACCAACAGT AGTCATCATC TTCTCTTAGC CACTGGGTCA 
    AGTGACTGCT TCCTCAAACT TTGGGATTTG AATCAAAAAG AATGTCGAAA TACCATGTTT 
    GGTCATACAA ATTCAGTCAA TCACTGCAGA TTTTCACCAG ATGATAAGCT TTTGGCTAGT 
    TGTTCAGCTG ATGGAACCTT AAAGCTTTGG GATGCGACAT CAGCAAATGA GAGGAAAAGC 
    ATTAATGTGA AACAGTTCTT CCTAAATTTG GAGGACCCTC AAGAGGATAT GGAAGTGATA 
    GTGAAGTGTT GTTCGTGGTC TGCTGATGGT GCAAGGATAA TGGTGGCAGC AAAAAATAAA 
    ATCTTTCTTT TTGACATTCA TACTAGTGGC CTATTGGGAG AAATCCACAC GGGCCATCAC 
    AGCACCATCC AGTACTGTGA CTTCTCCCCA CAAAACCATT TGGCAGTGGT TGCTTTGTCC 
    CAGTACTGTG TAGAGTTGTG GAATACAGAC TCACGTTCAA AGGTGGCTGA TTGCAGAGGA 
    CATTTAAGTT GGGTTCATGG TGTGATGTTT TCTCCTGATG GATCATCATT TTTGACATCT 
    TCTGATGACC AGACAATCAG GCTCTGGGAG ACAAAGAAAG TATGTAAGAA CTCTGCTGTA 
    ATGTTAAAGC AAGAAGTAGA TGTTGTGTTT CAAGAAAATG AAGTGATGGT CCTTGCAGTT 
    GACCATATAA GACGTCTGCA ACTCATTAAT GGAAGAACAG GTCAGATTGA TTATCTGACT 
    GAAGCTCAAG TTAGCTGCTG TTGCTTAAGT CCACATCTTC AGTACATTGC ATTTGGAGAT 
    GAAAATGGAG CCATTGAGAT TTTAGAACTT GTAAACAATA GAATCTTCCA GTCCAGGTTT 
    CAGCACAAGA AAACTGTATG GCACATCCAG TTCACAGCCG ATGAGAAGAC TCTTATTTCA 
    AGTTCTGATG ATGCTGAAAT TCAGGTATGG AATTGGCAAT TGGACAAATG TATCTTTCTA 
    CGAGGCCATC AGGAAACAGT GAAAGACTTT AGACTCTTGA AAAATTCAAG ACTGCTTTCT 
    TGGTCATTTG ATGGAACAGT GAAGGTATGG AATATTATTA CTGGAAATAA AGAAAAAGAC 
    TTTGTCTGTC ACCAGGGTAC AGTACTTTCT TGTGACATTT CTCACGATGC TACCAAGTTT 
    TCATCTACCT CTGCTGACAA GACTGCAAAG ATCTGGAGTT TTGATCTCCT TTTGCCACTT 
    CATGAATTGA GGGGCCACAA CGGCTGTGTG CGCTGCTCTG CCTTCTCTGT GGACAGTACC 
    CTGCTGGCAA CGGGAGATGA CAATGGAGAA ATCAGGATAT GGAATGTCTC AAACGGTGAG 
    CTTCTTCATT TGTGTGCTCC GCTTTCAGAA GAAGGAGCTG CTACCCATGG AGGCTGGGTG 
    ACTGACCTTT GCTTTTCTCC AGATGGCAAA ATGCTTATCT CTGCTGGAGG ATATATTAAG 
    TGGTGGAACG TTGTCACTGG GGAATCCTCA CAGACCTTCT ACACAAATGG AACCAATCTT 
    AAGAAAATAC ACGTGTCCCC TGACTTCAAA ACATATGTGA CTGTGGATAA TCTTGGTATT 
    TTATATATTT TACAGACTTT AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_863651.1
    CDS578..4324
    Translation

    Target ORF information:

    RefSeq Version NM_181861.1
    Organism Homo sapiens(human)
    Definition Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_181861.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGGTAAA 
    GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
    CAGAGGCCAG TTGTTTTTGT CACAAGGAAG AAGCTGGTGA ATGCAATTCA GCAGAAGCTC 
    TCCAAATTGA AAGGTGAACC AGGATGGGTC ACCATACATG GAATGGCAGG CTGTGGGAAG 
    TCTGTATTAG CTGCAGAAGC TGTTAGAGAT CATTCCCTTT TAGAAGGTTG TTTCCCAGGG 
    GGAGTGCATT GGGTTTCAGT TGGGAAACAA GACAAATCTG GGCTTCTGAT GAAACTGCAG 
    AATCTTTGCA CACGGTTGGA TCAGGATGAG AGTTTTTCCC AGAGGCTTCC ACTTAATATT 
    GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTTCGCA AACACCCAAG GTCTCTCTTG 
    ATCTTGGATG ATGTTTGGGA CTCTTGGGTG TTGAAAGCTT TTGACAGTCA GTGTCAGATT 
    CTTCTTACAA CCAGAGACAA GAGTGTTACA GATTCAGTAA TGGGTCCTAA ATATGTAGTC 
    CCTGTGGAGA GTTCCTTAGG AAAGGAAAAA GGACTTGAAA TTTTATCCCT TTTTGTTAAT 
    ATGAAGAAGG CAGATTTGCC AGAACAAGCT CATAGTATTA TAAAAGAATG TAAAGGCTCT 
    CCCCTTGTAG TATCTTTAAT TGGTGCACTT TTACGTGATT TTCCCAATCG CTGGGAGTAC 
    TACCTCAAAC AGCTTCAGAA TAAGCAGTTT AAGAGAATAA GGAAATCTTC GTCTTATGAT 
    TATGAGGCTC TAGATGAAGC CATGTCTATA AGTGTTGAAA TGCTCAGAGA AGACATCAAA 
    GATTATTACA CAGATCTTTC CATCCTTCAG AAGGACGTTA AGGTGCCTAC AAAGGTGTTA 
    TGTATTCTCT GGGACATGGA AACTGAAGAA GTTGAAGACA TACTGCAGGA GTTTGTAAAT 
    AAGTCTCTTT TATTCTGTGA TCGGAATGGA AAGTCGTTTC GTTATTATTT ACATGATCTT 
    CAAGTAGATT TTCTTACAGA GAAGAATTGC AGCCAGCTTC AGGATCTACA TAAGAAGATA 
    ATCACTCAGT TTCAGAGATA TCACCAGCCG CATACTCTTT CACCAGATCA GGAAGACTGT 
    ATGTATTGGT ACAACTTTCT GGCCTATCAC ATGGCCAGTG CCAAGATGCA CAAGGAACTT 
    TGTGCTTTAA TGTTTTCCCT GGATTGGATT AAAGCAAAAA CAGAACTTGT AGGCCCTGCT 
    CATCTGATTC ATGAATTTGT GGAATACAGA CATATACTAG ATGAAAAGGA TTGTGCAGTC 
    AGTGAGAATT TTCAGGAGTT TTTATCTTTA AATGGACACC TTCTTGGACG ACAGCCATTT 
    CCTAATATTG TACAACTGGG TCTCTGTGAG CCGGAAACTT CAGAAGTTTA TCAGCAAGCT 
    AAGCTGCAGG CCAAGCAGGA GGTCGATAAT GGAATGCTTT ACCTGGAATG GATAAACAAA 
    AAAAACATCA CGAATCTTTC CCGCTTAGTT GTCCGCCCCC ACACAGATGC TGTTTACCAT 
    GCCTGCTTTT CTGAGGATGG TCAGAGAATA GCTTCTTGTG GAGCTGATAA AACCTTACAG 
    GTGTTCAAAG CTGAAACAGG AGAGAAACTT CTAGAAATCA AGGCTCATGA GGATGAAGTG 
    CTTTGTTGTG CATTCTCTAC AGATGACAGA TTTATAGCAA CCTGCTCAGT GGATAAAAAA 
    GTGAAGATTT GGAATTCTAT GACTGGGGAA CTAGTACACA CCTATGATGA GCACTCAGAG 
    CAAGTCAATT GCTGCCATTT CACCAACAGT AGTCATCATC TTCTCTTAGC CACTGGGTCA 
    AGTGACTGCT TCCTCAAACT TTGGGATTTG AATCAAAAAG AATGTCGAAA TACCATGTTT 
    GGTCATACAA ATTCAGTCAA TCACTGCAGA TTTTCACCAG ATGATAAGCT TTTGGCTAGT 
    TGTTCAGCTG ATGGAACCTT AAAGCTTTGG GATGCGACAT CAGCAAATGA GAGGAAAAGC 
    ATTAATGTGA AACAGTTCTT CCTAAATTTG GAGGACCCTC AAGAGGATAT GGAAGTGATA 
    GTGAAGTGTT GTTCGTGGTC TGCTGATGGT GCAAGGATAA TGGTGGCAGC AAAAAATAAA 
    ATCTTTCTTT TTGACATTCA TACTAGTGGC CTATTGGGAG AAATCCACAC GGGCCATCAC 
    AGCACCATCC AGTACTGTGA CTTCTCCCCA CAAAACCATT TGGCAGTGGT TGCTTTGTCC 
    CAGTACTGTG TAGAGTTGTG GAATACAGAC TCACGTTCAA AGGTGGCTGA TTGCAGAGGA 
    CATTTAAGTT GGGTTCATGG TGTGATGTTT TCTCCTGATG GATCATCATT TTTGACATCT 
    TCTGATGACC AGACAATCAG GCTCTGGGAG ACAAAGAAAG TATGTAAGAA CTCTGCTGTA 
    ATGTTAAAGC AAGAAGTAGA TGTTGTGTTT CAAGAAAATG AAGTGATGGT CCTTGCAGTT 
    GACCATATAA GACGTCTGCA ACTCATTAAT GGAAGAACAG GTCAGATTGA TTATCTGACT 
    GAAGCTCAAG TTAGCTGCTG TTGCTTAAGT CCACATCTTC AGTACATTGC ATTTGGAGAT 
    GAAAATGGAG CCATTGAGAT TTTAGAACTT GTAAACAATA GAATCTTCCA GTCCAGGTTT 
    CAGCACAAGA AAACTGTATG GCACATCCAG TTCACAGCCG ATGAGAAGAC TCTTATTTCA 
    AGTTCTGATG ATGCTGAAAT TCAGGTATGG AATTGGCAAT TGGACAAATG TATCTTTCTA 
    CGAGGCCATC AGGAAACAGT GAAAGACTTT AGACTCTTGA AAAATTCAAG ACTGCTTTCT 
    TGGTCATTTG ATGGAACAGT GAAGGTATGG AATATTATTA CTGGAAATAA AGAAAAAGAC 
    TTTGTCTGTC ACCAGGGTAC AGTACTTTCT TGTGACATTT CTCACGATGC TACCAAGTTT 
    TCATCTACCT CTGCTGACAA GACTGCAAAG ATCTGGAGTT TTGATCTCCT TTTGCCACTT 
    CATGAATTGA GGGGCCACAA CGGCTGTGTG CGCTGCTCTG CCTTCTCTGT GGACAGTACC 
    CTGCTGGCAA CGGGAGATGA CAATGGAGAA ATCAGGATAT GGAATGTCTC AAACGGTGAG 
    CTTCTTCATT TGTGTGCTCC GCTTTCAGAA GAAGGAGCTG CTACCCATGG AGGCTGGGTG 
    ACTGACCTTT GCTTTTCTCC AGATGGCAAA ATGCTTATCT CTGCTGGAGG ATATATTAAG 
    TGGTGGAACG TTGTCACTGG GGAATCCTCA CAGACCTTCT ACACAAATGG AACCAATCTT 
    AAGAAAATAC ACGTGTCCCC TGACTTCAAA ACATATGTGA CTGTGGATAA TCTTGGTATT 
    TTATATATTT TACAGACTTT AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    APAF1 ( NM_181861.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_863651.1 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 3, mRNA.
    Price & Availability↑
    CloneID OHu20629
    Clone ID Related Accession (Same CDS sequence) NM_181861.1 , NM_181861.2
    Accession Version NM_181861.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3747bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product apoptotic protease-activating factor 1 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC013283.23, AF013263.1, AF149794.1 and AC011248.8. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_181861.1. Transcript Variant: This variant (3), also known as Apaf-1L, encodes the longest isoform (c) of this protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.187595.1, SRR1803614.51709.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGGTAAA 
    GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
    CAGAGGCCAG TTGTTTTTGT CACAAGGAAG AAGCTGGTGA ATGCAATTCA GCAGAAGCTC 
    TCCAAATTGA AAGGTGAACC AGGATGGGTC ACCATACATG GAATGGCAGG CTGTGGGAAG 
    TCTGTATTAG CTGCAGAAGC TGTTAGAGAT CATTCCCTTT TAGAAGGTTG TTTCCCAGGG 
    GGAGTGCATT GGGTTTCAGT TGGGAAACAA GACAAATCTG GGCTTCTGAT GAAACTGCAG 
    AATCTTTGCA CACGGTTGGA TCAGGATGAG AGTTTTTCCC AGAGGCTTCC ACTTAATATT 
    GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTTCGCA AACACCCAAG GTCTCTCTTG 
    ATCTTGGATG ATGTTTGGGA CTCTTGGGTG TTGAAAGCTT TTGACAGTCA GTGTCAGATT 
    CTTCTTACAA CCAGAGACAA GAGTGTTACA GATTCAGTAA TGGGTCCTAA ATATGTAGTC 
    CCTGTGGAGA GTTCCTTAGG AAAGGAAAAA GGACTTGAAA TTTTATCCCT TTTTGTTAAT 
    ATGAAGAAGG CAGATTTGCC AGAACAAGCT CATAGTATTA TAAAAGAATG TAAAGGCTCT 
    CCCCTTGTAG TATCTTTAAT TGGTGCACTT TTACGTGATT TTCCCAATCG CTGGGAGTAC 
    TACCTCAAAC AGCTTCAGAA TAAGCAGTTT AAGAGAATAA GGAAATCTTC GTCTTATGAT 
    TATGAGGCTC TAGATGAAGC CATGTCTATA AGTGTTGAAA TGCTCAGAGA AGACATCAAA 
    GATTATTACA CAGATCTTTC CATCCTTCAG AAGGACGTTA AGGTGCCTAC AAAGGTGTTA 
    TGTATTCTCT GGGACATGGA AACTGAAGAA GTTGAAGACA TACTGCAGGA GTTTGTAAAT 
    AAGTCTCTTT TATTCTGTGA TCGGAATGGA AAGTCGTTTC GTTATTATTT ACATGATCTT 
    CAAGTAGATT TTCTTACAGA GAAGAATTGC AGCCAGCTTC AGGATCTACA TAAGAAGATA 
    ATCACTCAGT TTCAGAGATA TCACCAGCCG CATACTCTTT CACCAGATCA GGAAGACTGT 
    ATGTATTGGT ACAACTTTCT GGCCTATCAC ATGGCCAGTG CCAAGATGCA CAAGGAACTT 
    TGTGCTTTAA TGTTTTCCCT GGATTGGATT AAAGCAAAAA CAGAACTTGT AGGCCCTGCT 
    CATCTGATTC ATGAATTTGT GGAATACAGA CATATACTAG ATGAAAAGGA TTGTGCAGTC 
    AGTGAGAATT TTCAGGAGTT TTTATCTTTA AATGGACACC TTCTTGGACG ACAGCCATTT 
    CCTAATATTG TACAACTGGG TCTCTGTGAG CCGGAAACTT CAGAAGTTTA TCAGCAAGCT 
    AAGCTGCAGG CCAAGCAGGA GGTCGATAAT GGAATGCTTT ACCTGGAATG GATAAACAAA 
    AAAAACATCA CGAATCTTTC CCGCTTAGTT GTCCGCCCCC ACACAGATGC TGTTTACCAT 
    GCCTGCTTTT CTGAGGATGG TCAGAGAATA GCTTCTTGTG GAGCTGATAA AACCTTACAG 
    GTGTTCAAAG CTGAAACAGG AGAGAAACTT CTAGAAATCA AGGCTCATGA GGATGAAGTG 
    CTTTGTTGTG CATTCTCTAC AGATGACAGA TTTATAGCAA CCTGCTCAGT GGATAAAAAA 
    GTGAAGATTT GGAATTCTAT GACTGGGGAA CTAGTACACA CCTATGATGA GCACTCAGAG 
    CAAGTCAATT GCTGCCATTT CACCAACAGT AGTCATCATC TTCTCTTAGC CACTGGGTCA 
    AGTGACTGCT TCCTCAAACT TTGGGATTTG AATCAAAAAG AATGTCGAAA TACCATGTTT 
    GGTCATACAA ATTCAGTCAA TCACTGCAGA TTTTCACCAG ATGATAAGCT TTTGGCTAGT 
    TGTTCAGCTG ATGGAACCTT AAAGCTTTGG GATGCGACAT CAGCAAATGA GAGGAAAAGC 
    ATTAATGTGA AACAGTTCTT CCTAAATTTG GAGGACCCTC AAGAGGATAT GGAAGTGATA 
    GTGAAGTGTT GTTCGTGGTC TGCTGATGGT GCAAGGATAA TGGTGGCAGC AAAAAATAAA 
    ATCTTTCTTT TTGACATTCA TACTAGTGGC CTATTGGGAG AAATCCACAC GGGCCATCAC 
    AGCACCATCC AGTACTGTGA CTTCTCCCCA CAAAACCATT TGGCAGTGGT TGCTTTGTCC 
    CAGTACTGTG TAGAGTTGTG GAATACAGAC TCACGTTCAA AGGTGGCTGA TTGCAGAGGA 
    CATTTAAGTT GGGTTCATGG TGTGATGTTT TCTCCTGATG GATCATCATT TTTGACATCT 
    TCTGATGACC AGACAATCAG GCTCTGGGAG ACAAAGAAAG TATGTAAGAA CTCTGCTGTA 
    ATGTTAAAGC AAGAAGTAGA TGTTGTGTTT CAAGAAAATG AAGTGATGGT CCTTGCAGTT 
    GACCATATAA GACGTCTGCA ACTCATTAAT GGAAGAACAG GTCAGATTGA TTATCTGACT 
    GAAGCTCAAG TTAGCTGCTG TTGCTTAAGT CCACATCTTC AGTACATTGC ATTTGGAGAT 
    GAAAATGGAG CCATTGAGAT TTTAGAACTT GTAAACAATA GAATCTTCCA GTCCAGGTTT 
    CAGCACAAGA AAACTGTATG GCACATCCAG TTCACAGCCG ATGAGAAGAC TCTTATTTCA 
    AGTTCTGATG ATGCTGAAAT TCAGGTATGG AATTGGCAAT TGGACAAATG TATCTTTCTA 
    CGAGGCCATC AGGAAACAGT GAAAGACTTT AGACTCTTGA AAAATTCAAG ACTGCTTTCT 
    TGGTCATTTG ATGGAACAGT GAAGGTATGG AATATTATTA CTGGAAATAA AGAAAAAGAC 
    TTTGTCTGTC ACCAGGGTAC AGTACTTTCT TGTGACATTT CTCACGATGC TACCAAGTTT 
    TCATCTACCT CTGCTGACAA GACTGCAAAG ATCTGGAGTT TTGATCTCCT TTTGCCACTT 
    CATGAATTGA GGGGCCACAA CGGCTGTGTG CGCTGCTCTG CCTTCTCTGT GGACAGTACC 
    CTGCTGGCAA CGGGAGATGA CAATGGAGAA ATCAGGATAT GGAATGTCTC AAACGGTGAG 
    CTTCTTCATT TGTGTGCTCC GCTTTCAGAA GAAGGAGCTG CTACCCATGG AGGCTGGGTG 
    ACTGACCTTT GCTTTTCTCC AGATGGCAAA ATGCTTATCT CTGCTGGAGG ATATATTAAG 
    TGGTGGAACG TTGTCACTGG GGAATCCTCA CAGACCTTCT ACACAAATGG AACCAATCTT 
    AAGAAAATAC ACGTGTCCCC TGACTTCAAA ACATATGTGA CTGTGGATAA TCTTGGTATT 
    TTATATATTT TACAGACTTT AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_863651.1
    CDS588..4334
    Translation

    Target ORF information:

    RefSeq Version NM_181861.2
    Organism Homo sapiens(human)
    Definition Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_181861.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGGTAAA 
    GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
    CAGAGGCCAG TTGTTTTTGT CACAAGGAAG AAGCTGGTGA ATGCAATTCA GCAGAAGCTC 
    TCCAAATTGA AAGGTGAACC AGGATGGGTC ACCATACATG GAATGGCAGG CTGTGGGAAG 
    TCTGTATTAG CTGCAGAAGC TGTTAGAGAT CATTCCCTTT TAGAAGGTTG TTTCCCAGGG 
    GGAGTGCATT GGGTTTCAGT TGGGAAACAA GACAAATCTG GGCTTCTGAT GAAACTGCAG 
    AATCTTTGCA CACGGTTGGA TCAGGATGAG AGTTTTTCCC AGAGGCTTCC ACTTAATATT 
    GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTTCGCA AACACCCAAG GTCTCTCTTG 
    ATCTTGGATG ATGTTTGGGA CTCTTGGGTG TTGAAAGCTT TTGACAGTCA GTGTCAGATT 
    CTTCTTACAA CCAGAGACAA GAGTGTTACA GATTCAGTAA TGGGTCCTAA ATATGTAGTC 
    CCTGTGGAGA GTTCCTTAGG AAAGGAAAAA GGACTTGAAA TTTTATCCCT TTTTGTTAAT 
    ATGAAGAAGG CAGATTTGCC AGAACAAGCT CATAGTATTA TAAAAGAATG TAAAGGCTCT 
    CCCCTTGTAG TATCTTTAAT TGGTGCACTT TTACGTGATT TTCCCAATCG CTGGGAGTAC 
    TACCTCAAAC AGCTTCAGAA TAAGCAGTTT AAGAGAATAA GGAAATCTTC GTCTTATGAT 
    TATGAGGCTC TAGATGAAGC CATGTCTATA AGTGTTGAAA TGCTCAGAGA AGACATCAAA 
    GATTATTACA CAGATCTTTC CATCCTTCAG AAGGACGTTA AGGTGCCTAC AAAGGTGTTA 
    TGTATTCTCT GGGACATGGA AACTGAAGAA GTTGAAGACA TACTGCAGGA GTTTGTAAAT 
    AAGTCTCTTT TATTCTGTGA TCGGAATGGA AAGTCGTTTC GTTATTATTT ACATGATCTT 
    CAAGTAGATT TTCTTACAGA GAAGAATTGC AGCCAGCTTC AGGATCTACA TAAGAAGATA 
    ATCACTCAGT TTCAGAGATA TCACCAGCCG CATACTCTTT CACCAGATCA GGAAGACTGT 
    ATGTATTGGT ACAACTTTCT GGCCTATCAC ATGGCCAGTG CCAAGATGCA CAAGGAACTT 
    TGTGCTTTAA TGTTTTCCCT GGATTGGATT AAAGCAAAAA CAGAACTTGT AGGCCCTGCT 
    CATCTGATTC ATGAATTTGT GGAATACAGA CATATACTAG ATGAAAAGGA TTGTGCAGTC 
    AGTGAGAATT TTCAGGAGTT TTTATCTTTA AATGGACACC TTCTTGGACG ACAGCCATTT 
    CCTAATATTG TACAACTGGG TCTCTGTGAG CCGGAAACTT CAGAAGTTTA TCAGCAAGCT 
    AAGCTGCAGG CCAAGCAGGA GGTCGATAAT GGAATGCTTT ACCTGGAATG GATAAACAAA 
    AAAAACATCA CGAATCTTTC CCGCTTAGTT GTCCGCCCCC ACACAGATGC TGTTTACCAT 
    GCCTGCTTTT CTGAGGATGG TCAGAGAATA GCTTCTTGTG GAGCTGATAA AACCTTACAG 
    GTGTTCAAAG CTGAAACAGG AGAGAAACTT CTAGAAATCA AGGCTCATGA GGATGAAGTG 
    CTTTGTTGTG CATTCTCTAC AGATGACAGA TTTATAGCAA CCTGCTCAGT GGATAAAAAA 
    GTGAAGATTT GGAATTCTAT GACTGGGGAA CTAGTACACA CCTATGATGA GCACTCAGAG 
    CAAGTCAATT GCTGCCATTT CACCAACAGT AGTCATCATC TTCTCTTAGC CACTGGGTCA 
    AGTGACTGCT TCCTCAAACT TTGGGATTTG AATCAAAAAG AATGTCGAAA TACCATGTTT 
    GGTCATACAA ATTCAGTCAA TCACTGCAGA TTTTCACCAG ATGATAAGCT TTTGGCTAGT 
    TGTTCAGCTG ATGGAACCTT AAAGCTTTGG GATGCGACAT CAGCAAATGA GAGGAAAAGC 
    ATTAATGTGA AACAGTTCTT CCTAAATTTG GAGGACCCTC AAGAGGATAT GGAAGTGATA 
    GTGAAGTGTT GTTCGTGGTC TGCTGATGGT GCAAGGATAA TGGTGGCAGC AAAAAATAAA 
    ATCTTTCTTT TTGACATTCA TACTAGTGGC CTATTGGGAG AAATCCACAC GGGCCATCAC 
    AGCACCATCC AGTACTGTGA CTTCTCCCCA CAAAACCATT TGGCAGTGGT TGCTTTGTCC 
    CAGTACTGTG TAGAGTTGTG GAATACAGAC TCACGTTCAA AGGTGGCTGA TTGCAGAGGA 
    CATTTAAGTT GGGTTCATGG TGTGATGTTT TCTCCTGATG GATCATCATT TTTGACATCT 
    TCTGATGACC AGACAATCAG GCTCTGGGAG ACAAAGAAAG TATGTAAGAA CTCTGCTGTA 
    ATGTTAAAGC AAGAAGTAGA TGTTGTGTTT CAAGAAAATG AAGTGATGGT CCTTGCAGTT 
    GACCATATAA GACGTCTGCA ACTCATTAAT GGAAGAACAG GTCAGATTGA TTATCTGACT 
    GAAGCTCAAG TTAGCTGCTG TTGCTTAAGT CCACATCTTC AGTACATTGC ATTTGGAGAT 
    GAAAATGGAG CCATTGAGAT TTTAGAACTT GTAAACAATA GAATCTTCCA GTCCAGGTTT 
    CAGCACAAGA AAACTGTATG GCACATCCAG TTCACAGCCG ATGAGAAGAC TCTTATTTCA 
    AGTTCTGATG ATGCTGAAAT TCAGGTATGG AATTGGCAAT TGGACAAATG TATCTTTCTA 
    CGAGGCCATC AGGAAACAGT GAAAGACTTT AGACTCTTGA AAAATTCAAG ACTGCTTTCT 
    TGGTCATTTG ATGGAACAGT GAAGGTATGG AATATTATTA CTGGAAATAA AGAAAAAGAC 
    TTTGTCTGTC ACCAGGGTAC AGTACTTTCT TGTGACATTT CTCACGATGC TACCAAGTTT 
    TCATCTACCT CTGCTGACAA GACTGCAAAG ATCTGGAGTT TTGATCTCCT TTTGCCACTT 
    CATGAATTGA GGGGCCACAA CGGCTGTGTG CGCTGCTCTG CCTTCTCTGT GGACAGTACC 
    CTGCTGGCAA CGGGAGATGA CAATGGAGAA ATCAGGATAT GGAATGTCTC AAACGGTGAG 
    CTTCTTCATT TGTGTGCTCC GCTTTCAGAA GAAGGAGCTG CTACCCATGG AGGCTGGGTG 
    ACTGACCTTT GCTTTTCTCC AGATGGCAAA ATGCTTATCT CTGCTGGAGG ATATATTAAG 
    TGGTGGAACG TTGTCACTGG GGAATCCTCA CAGACCTTCT ACACAAATGG AACCAATCTT 
    AAGAAAATAC ACGTGTCCCC TGACTTCAAA ACATATGTGA CTGTGGATAA TCTTGGTATT 
    TTATATATTT TACAGACTTT AGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    APAF1 ( NM_013229.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_037361.1 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu20835
    Clone ID Related Accession (Same CDS sequence) NM_013229.3 , NM_013229.2
    Accession Version NM_013229.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product apoptotic protease-activating factor 1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC013283.23, AF013263.1, AF134397.1 and AC011248.8. On Jun 2, 2019 this sequence version replaced NM_013229.2. Transcript Variant: This variant (1) uses an alternate splice site in the coding region but maintains the reading frame, compared to variant 3. Variant 1 encodes isoform a, which is shorter than isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB007873.2, BC150255.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGTAAGG 
    ACAGTCCTGT GTGAAGGTGG AGTACCACAG AGGCCAGTTG TTTTTGTCAC AAGGAAGAAG 
    CTGGTGAATG CAATTCAGCA GAAGCTCTCC AAATTGAAAG GTGAACCAGG ATGGGTCACC 
    ATACATGGAA TGGCAGGCTG TGGGAAGTCT GTATTAGCTG CAGAAGCTGT TAGAGATCAT 
    TCCCTTTTAG AAGGTTGTTT CCCAGGGGGA GTGCATTGGG TTTCAGTTGG GAAACAAGAC 
    AAATCTGGGC TTCTGATGAA ACTGCAGAAT CTTTGCACAC GGTTGGATCA GGATGAGAGT 
    TTTTCCCAGA GGCTTCCACT TAATATTGAA GAGGCTAAAG ACCGTCTCCG CATTCTGATG 
    CTTCGCAAAC ACCCAAGGTC TCTCTTGATC TTGGATGATG TTTGGGACTC TTGGGTGTTG 
    AAAGCTTTTG ACAGTCAGTG TCAGATTCTT CTTACAACCA GAGACAAGAG TGTTACAGAT 
    TCAGTAATGG GTCCTAAATA TGTAGTCCCT GTGGAGAGTT CCTTAGGAAA GGAAAAAGGA 
    CTTGAAATTT TATCCCTTTT TGTTAATATG AAGAAGGCAG ATTTGCCAGA ACAAGCTCAT 
    AGTATTATAA AAGAATGTAA AGGCTCTCCC CTTGTAGTAT CTTTAATTGG TGCACTTTTA 
    CGTGATTTTC CCAATCGCTG GGAGTACTAC CTCAAACAGC TTCAGAATAA GCAGTTTAAG 
    AGAATAAGGA AATCTTCGTC TTATGATTAT GAGGCTCTAG ATGAAGCCAT GTCTATAAGT 
    GTTGAAATGC TCAGAGAAGA CATCAAAGAT TATTACACAG ATCTTTCCAT CCTTCAGAAG 
    GACGTTAAGG TGCCTACAAA GGTGTTATGT ATTCTCTGGG ACATGGAAAC TGAAGAAGTT 
    GAAGACATAC TGCAGGAGTT TGTAAATAAG TCTCTTTTAT TCTGTGATCG GAATGGAAAG 
    TCGTTTCGTT ATTATTTACA TGATCTTCAA GTAGATTTTC TTACAGAGAA GAATTGCAGC 
    CAGCTTCAGG ATCTACATAA GAAGATAATC ACTCAGTTTC AGAGATATCA CCAGCCGCAT 
    ACTCTTTCAC CAGATCAGGA AGACTGTATG TATTGGTACA ACTTTCTGGC CTATCACATG 
    GCCAGTGCCA AGATGCACAA GGAACTTTGT GCTTTAATGT TTTCCCTGGA TTGGATTAAA 
    GCAAAAACAG AACTTGTAGG CCCTGCTCAT CTGATTCATG AATTTGTGGA ATACAGACAT 
    ATACTAGATG AAAAGGATTG TGCAGTCAGT GAGAATTTTC AGGAGTTTTT ATCTTTAAAT 
    GGACACCTTC TTGGACGACA GCCATTTCCT AATATTGTAC AACTGGGTCT CTGTGAGCCG 
    GAAACTTCAG AAGTTTATCA GCAAGCTAAG CTGCAGGCCA AGCAGGAGGT CGATAATGGA 
    ATGCTTTACC TGGAATGGAT AAACAAAAAA AACATCACGA ATCTTTCCCG CTTAGTTGTC 
    CGCCCCCACA CAGATGCTGT TTACCATGCC TGCTTTTCTG AGGATGGTCA GAGAATAGCT 
    TCTTGTGGAG CTGATAAAAC CTTACAGGTG TTCAAAGCTG AAACAGGAGA GAAACTTCTA 
    GAAATCAAGG CTCATGAGGA TGAAGTGCTT TGTTGTGCAT TCTCTACAGA TGACAGATTT 
    ATAGCAACCT GCTCAGTGGA TAAAAAAGTG AAGATTTGGA ATTCTATGAC TGGGGAACTA 
    GTACACACCT ATGATGAGCA CTCAGAGCAA GTCAATTGCT GCCATTTCAC CAACAGTAGT 
    CATCATCTTC TCTTAGCCAC TGGGTCAAGT GACTGCTTCC TCAAACTTTG GGATTTGAAT 
    CAAAAAGAAT GTCGAAATAC CATGTTTGGT CATACAAATT CAGTCAATCA CTGCAGATTT 
    TCACCAGATG ATAAGCTTTT GGCTAGTTGT TCAGCTGATG GAACCTTAAA GCTTTGGGAT 
    GCGACATCAG CAAATGAGAG GAAAAGCATT AATGTGAAAC AGTTCTTCCT AAATTTGGAG 
    GACCCTCAAG AGGATATGGA AGTGATAGTG AAGTGTTGTT CGTGGTCTGC TGATGGTGCA 
    AGGATAATGG TGGCAGCAAA AAATAAAATC TTTCTTTTTG ACATTCATAC TAGTGGCCTA 
    TTGGGAGAAA TCCACACGGG CCATCACAGC ACCATCCAGT ACTGTGACTT CTCCCCACAA 
    AACCATTTGG CAGTGGTTGC TTTGTCCCAG TACTGTGTAG AGTTGTGGAA TACAGACTCA 
    CGTTCAAAGG TGGCTGATTG CAGAGGACAT TTAAGTTGGG TTCATGGTGT GATGTTTTCT 
    CCTGATGGAT CATCATTTTT GACATCTTCT GATGACCAGA CAATCAGGCT CTGGGAGACA 
    AAGAAAGTAT GTAAGAACTC TGCTGTAATG TTAAAGCAAG AAGTAGATGT TGTGTTTCAA 
    GAAAATGAAG TGATGGTCCT TGCAGTTGAC CATATAAGAC GTCTGCAACT CATTAATGGA 
    AGAACAGGTC AGATTGATTA TCTGACTGAA GCTCAAGTTA GCTGCTGTTG CTTAAGTCCA 
    CATCTTCAGT ACATTGCATT TGGAGATGAA AATGGAGCCA TTGAGATTTT AGAACTTGTA 
    AACAATAGAA TCTTCCAGTC CAGGTTTCAG CACAAGAAAA CTGTATGGCA CATCCAGTTC 
    ACAGCCGATG AGAAGACTCT TATTTCAAGT TCTGATGATG CTGAAATTCA GGTATGGAAT 
    TGGCAATTGG ACAAATGTAT CTTTCTACGA GGCCATCAGG AAACAGTGAA AGACTTTAGA 
    CTCTTGAAAA ATTCAAGACT GCTTTCTTGG TCATTTGATG GAACAGTGAA GGTATGGAAT 
    ATTATTACTG GAAATAAAGA AAAAGACTTT GTCTGTCACC AGGGTACAGT ACTTTCTTGT 
    GACATTTCTC ACGATGCTAC CAAGTTTTCA TCTACCTCTG CTGACAAGAC TGCAAAGATC 
    TGGAGTTTTG ATCTCCTTTT GCCACTTCAT GAATTGAGGG GCCACAACGG CTGTGTGCGC 
    TGCTCTGCCT TCTCTGTGGA CAGTACCCTG CTGGCAACGG GAGATGACAA TGGAGAAATC 
    AGGATATGGA ATGTCTCAAA CGGTGAGCTT CTTCATTTGT GTGCTCCGCT TTCAGAAGAA 
    GGAGCTGCTA CCCATGGAGG CTGGGTGACT GACCTTTGCT TTTCTCCAGA TGGCAAAATG 
    CTTATCTCTG CTGGAGGATA TATTAAGTGG TGGAACGTTG TCACTGGGGA ATCCTCACAG 
    ACCTTCTACA CAAATGGAAC CAATCTTAAG AAAATACACG TGTCCCCTGA CTTCAAAACA 
    TATGTGACTG TGGATAATCT TGGTATTTTA TATATTTTAC AGACTTTAGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_037361.1
    CDS588..4301
    Translation

    Target ORF information:

    RefSeq Version NM_013229.3
    Organism Homo sapiens(human)
    Definition Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_013229.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGTAAGG 
    ACAGTCCTGT GTGAAGGTGG AGTACCACAG AGGCCAGTTG TTTTTGTCAC AAGGAAGAAG 
    CTGGTGAATG CAATTCAGCA GAAGCTCTCC AAATTGAAAG GTGAACCAGG ATGGGTCACC 
    ATACATGGAA TGGCAGGCTG TGGGAAGTCT GTATTAGCTG CAGAAGCTGT TAGAGATCAT 
    TCCCTTTTAG AAGGTTGTTT CCCAGGGGGA GTGCATTGGG TTTCAGTTGG GAAACAAGAC 
    AAATCTGGGC TTCTGATGAA ACTGCAGAAT CTTTGCACAC GGTTGGATCA GGATGAGAGT 
    TTTTCCCAGA GGCTTCCACT TAATATTGAA GAGGCTAAAG ACCGTCTCCG CATTCTGATG 
    CTTCGCAAAC ACCCAAGGTC TCTCTTGATC TTGGATGATG TTTGGGACTC TTGGGTGTTG 
    AAAGCTTTTG ACAGTCAGTG TCAGATTCTT CTTACAACCA GAGACAAGAG TGTTACAGAT 
    TCAGTAATGG GTCCTAAATA TGTAGTCCCT GTGGAGAGTT CCTTAGGAAA GGAAAAAGGA 
    CTTGAAATTT TATCCCTTTT TGTTAATATG AAGAAGGCAG ATTTGCCAGA ACAAGCTCAT 
    AGTATTATAA AAGAATGTAA AGGCTCTCCC CTTGTAGTAT CTTTAATTGG TGCACTTTTA 
    CGTGATTTTC CCAATCGCTG GGAGTACTAC CTCAAACAGC TTCAGAATAA GCAGTTTAAG 
    AGAATAAGGA AATCTTCGTC TTATGATTAT GAGGCTCTAG ATGAAGCCAT GTCTATAAGT 
    GTTGAAATGC TCAGAGAAGA CATCAAAGAT TATTACACAG ATCTTTCCAT CCTTCAGAAG 
    GACGTTAAGG TGCCTACAAA GGTGTTATGT ATTCTCTGGG ACATGGAAAC TGAAGAAGTT 
    GAAGACATAC TGCAGGAGTT TGTAAATAAG TCTCTTTTAT TCTGTGATCG GAATGGAAAG 
    TCGTTTCGTT ATTATTTACA TGATCTTCAA GTAGATTTTC TTACAGAGAA GAATTGCAGC 
    CAGCTTCAGG ATCTACATAA GAAGATAATC ACTCAGTTTC AGAGATATCA CCAGCCGCAT 
    ACTCTTTCAC CAGATCAGGA AGACTGTATG TATTGGTACA ACTTTCTGGC CTATCACATG 
    GCCAGTGCCA AGATGCACAA GGAACTTTGT GCTTTAATGT TTTCCCTGGA TTGGATTAAA 
    GCAAAAACAG AACTTGTAGG CCCTGCTCAT CTGATTCATG AATTTGTGGA ATACAGACAT 
    ATACTAGATG AAAAGGATTG TGCAGTCAGT GAGAATTTTC AGGAGTTTTT ATCTTTAAAT 
    GGACACCTTC TTGGACGACA GCCATTTCCT AATATTGTAC AACTGGGTCT CTGTGAGCCG 
    GAAACTTCAG AAGTTTATCA GCAAGCTAAG CTGCAGGCCA AGCAGGAGGT CGATAATGGA 
    ATGCTTTACC TGGAATGGAT AAACAAAAAA AACATCACGA ATCTTTCCCG CTTAGTTGTC 
    CGCCCCCACA CAGATGCTGT TTACCATGCC TGCTTTTCTG AGGATGGTCA GAGAATAGCT 
    TCTTGTGGAG CTGATAAAAC CTTACAGGTG TTCAAAGCTG AAACAGGAGA GAAACTTCTA 
    GAAATCAAGG CTCATGAGGA TGAAGTGCTT TGTTGTGCAT TCTCTACAGA TGACAGATTT 
    ATAGCAACCT GCTCAGTGGA TAAAAAAGTG AAGATTTGGA ATTCTATGAC TGGGGAACTA 
    GTACACACCT ATGATGAGCA CTCAGAGCAA GTCAATTGCT GCCATTTCAC CAACAGTAGT 
    CATCATCTTC TCTTAGCCAC TGGGTCAAGT GACTGCTTCC TCAAACTTTG GGATTTGAAT 
    CAAAAAGAAT GTCGAAATAC CATGTTTGGT CATACAAATT CAGTCAATCA CTGCAGATTT 
    TCACCAGATG ATAAGCTTTT GGCTAGTTGT TCAGCTGATG GAACCTTAAA GCTTTGGGAT 
    GCGACATCAG CAAATGAGAG GAAAAGCATT AATGTGAAAC AGTTCTTCCT AAATTTGGAG 
    GACCCTCAAG AGGATATGGA AGTGATAGTG AAGTGTTGTT CGTGGTCTGC TGATGGTGCA 
    AGGATAATGG TGGCAGCAAA AAATAAAATC TTTCTTTTTG ACATTCATAC TAGTGGCCTA 
    TTGGGAGAAA TCCACACGGG CCATCACAGC ACCATCCAGT ACTGTGACTT CTCCCCACAA 
    AACCATTTGG CAGTGGTTGC TTTGTCCCAG TACTGTGTAG AGTTGTGGAA TACAGACTCA 
    CGTTCAAAGG TGGCTGATTG CAGAGGACAT TTAAGTTGGG TTCATGGTGT GATGTTTTCT 
    CCTGATGGAT CATCATTTTT GACATCTTCT GATGACCAGA CAATCAGGCT CTGGGAGACA 
    AAGAAAGTAT GTAAGAACTC TGCTGTAATG TTAAAGCAAG AAGTAGATGT TGTGTTTCAA 
    GAAAATGAAG TGATGGTCCT TGCAGTTGAC CATATAAGAC GTCTGCAACT CATTAATGGA 
    AGAACAGGTC AGATTGATTA TCTGACTGAA GCTCAAGTTA GCTGCTGTTG CTTAAGTCCA 
    CATCTTCAGT ACATTGCATT TGGAGATGAA AATGGAGCCA TTGAGATTTT AGAACTTGTA 
    AACAATAGAA TCTTCCAGTC CAGGTTTCAG CACAAGAAAA CTGTATGGCA CATCCAGTTC 
    ACAGCCGATG AGAAGACTCT TATTTCAAGT TCTGATGATG CTGAAATTCA GGTATGGAAT 
    TGGCAATTGG ACAAATGTAT CTTTCTACGA GGCCATCAGG AAACAGTGAA AGACTTTAGA 
    CTCTTGAAAA ATTCAAGACT GCTTTCTTGG TCATTTGATG GAACAGTGAA GGTATGGAAT 
    ATTATTACTG GAAATAAAGA AAAAGACTTT GTCTGTCACC AGGGTACAGT ACTTTCTTGT 
    GACATTTCTC ACGATGCTAC CAAGTTTTCA TCTACCTCTG CTGACAAGAC TGCAAAGATC 
    TGGAGTTTTG ATCTCCTTTT GCCACTTCAT GAATTGAGGG GCCACAACGG CTGTGTGCGC 
    TGCTCTGCCT TCTCTGTGGA CAGTACCCTG CTGGCAACGG GAGATGACAA TGGAGAAATC 
    AGGATATGGA ATGTCTCAAA CGGTGAGCTT CTTCATTTGT GTGCTCCGCT TTCAGAAGAA 
    GGAGCTGCTA CCCATGGAGG CTGGGTGACT GACCTTTGCT TTTCTCCAGA TGGCAAAATG 
    CTTATCTCTG CTGGAGGATA TATTAAGTGG TGGAACGTTG TCACTGGGGA ATCCTCACAG 
    ACCTTCTACA CAAATGGAAC CAATCTTAAG AAAATACACG TGTCCCCTGA CTTCAAAACA 
    TATGTGACTG TGGATAATCT TGGTATTTTA TATATTTTAC AGACTTTAGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    APAF1 ( NM_013229.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_037361.1 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 1, mRNA.
    Price & Availability↑
    CloneID OHu20835
    Clone ID Related Accession (Same CDS sequence) NM_013229.3 , NM_013229.2
    Accession Version NM_013229.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3714bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-18
    Organism Homo sapiens(human)
    Product apoptotic protease-activating factor 1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF013263.1, AF134397.1 and AC011248.8. On Jul 9, 2003 this sequence version replaced NM_013229.1. Transcript Variant: This variant (1) uses an alternate splice site in the coding region but maintains the reading frame, compared to variant 3. Variant 1 encodes isoform a, which is shorter than isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC150255.1, AB007873.2 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGTAAGG 
    ACAGTCCTGT GTGAAGGTGG AGTACCACAG AGGCCAGTTG TTTTTGTCAC AAGGAAGAAG 
    CTGGTGAATG CAATTCAGCA GAAGCTCTCC AAATTGAAAG GTGAACCAGG ATGGGTCACC 
    ATACATGGAA TGGCAGGCTG TGGGAAGTCT GTATTAGCTG CAGAAGCTGT TAGAGATCAT 
    TCCCTTTTAG AAGGTTGTTT CCCAGGGGGA GTGCATTGGG TTTCAGTTGG GAAACAAGAC 
    AAATCTGGGC TTCTGATGAA ACTGCAGAAT CTTTGCACAC GGTTGGATCA GGATGAGAGT 
    TTTTCCCAGA GGCTTCCACT TAATATTGAA GAGGCTAAAG ACCGTCTCCG CATTCTGATG 
    CTTCGCAAAC ACCCAAGGTC TCTCTTGATC TTGGATGATG TTTGGGACTC TTGGGTGTTG 
    AAAGCTTTTG ACAGTCAGTG TCAGATTCTT CTTACAACCA GAGACAAGAG TGTTACAGAT 
    TCAGTAATGG GTCCTAAATA TGTAGTCCCT GTGGAGAGTT CCTTAGGAAA GGAAAAAGGA 
    CTTGAAATTT TATCCCTTTT TGTTAATATG AAGAAGGCAG ATTTGCCAGA ACAAGCTCAT 
    AGTATTATAA AAGAATGTAA AGGCTCTCCC CTTGTAGTAT CTTTAATTGG TGCACTTTTA 
    CGTGATTTTC CCAATCGCTG GGAGTACTAC CTCAAACAGC TTCAGAATAA GCAGTTTAAG 
    AGAATAAGGA AATCTTCGTC TTATGATTAT GAGGCTCTAG ATGAAGCCAT GTCTATAAGT 
    GTTGAAATGC TCAGAGAAGA CATCAAAGAT TATTACACAG ATCTTTCCAT CCTTCAGAAG 
    GACGTTAAGG TGCCTACAAA GGTGTTATGT ATTCTCTGGG ACATGGAAAC TGAAGAAGTT 
    GAAGACATAC TGCAGGAGTT TGTAAATAAG TCTCTTTTAT TCTGTGATCG GAATGGAAAG 
    TCGTTTCGTT ATTATTTACA TGATCTTCAA GTAGATTTTC TTACAGAGAA GAATTGCAGC 
    CAGCTTCAGG ATCTACATAA GAAGATAATC ACTCAGTTTC AGAGATATCA CCAGCCGCAT 
    ACTCTTTCAC CAGATCAGGA AGACTGTATG TATTGGTACA ACTTTCTGGC CTATCACATG 
    GCCAGTGCCA AGATGCACAA GGAACTTTGT GCTTTAATGT TTTCCCTGGA TTGGATTAAA 
    GCAAAAACAG AACTTGTAGG CCCTGCTCAT CTGATTCATG AATTTGTGGA ATACAGACAT 
    ATACTAGATG AAAAGGATTG TGCAGTCAGT GAGAATTTTC AGGAGTTTTT ATCTTTAAAT 
    GGACACCTTC TTGGACGACA GCCATTTCCT AATATTGTAC AACTGGGTCT CTGTGAGCCG 
    GAAACTTCAG AAGTTTATCA GCAAGCTAAG CTGCAGGCCA AGCAGGAGGT CGATAATGGA 
    ATGCTTTACC TGGAATGGAT AAACAAAAAA AACATCACGA ATCTTTCCCG CTTAGTTGTC 
    CGCCCCCACA CAGATGCTGT TTACCATGCC TGCTTTTCTG AGGATGGTCA GAGAATAGCT 
    TCTTGTGGAG CTGATAAAAC CTTACAGGTG TTCAAAGCTG AAACAGGAGA GAAACTTCTA 
    GAAATCAAGG CTCATGAGGA TGAAGTGCTT TGTTGTGCAT TCTCTACAGA TGACAGATTT 
    ATAGCAACCT GCTCAGTGGA TAAAAAAGTG AAGATTTGGA ATTCTATGAC TGGGGAACTA 
    GTACACACCT ATGATGAGCA CTCAGAGCAA GTCAATTGCT GCCATTTCAC CAACAGTAGT 
    CATCATCTTC TCTTAGCCAC TGGGTCAAGT GACTGCTTCC TCAAACTTTG GGATTTGAAT 
    CAAAAAGAAT GTCGAAATAC CATGTTTGGT CATACAAATT CAGTCAATCA CTGCAGATTT 
    TCACCAGATG ATAAGCTTTT GGCTAGTTGT TCAGCTGATG GAACCTTAAA GCTTTGGGAT 
    GCGACATCAG CAAATGAGAG GAAAAGCATT AATGTGAAAC AGTTCTTCCT AAATTTGGAG 
    GACCCTCAAG AGGATATGGA AGTGATAGTG AAGTGTTGTT CGTGGTCTGC TGATGGTGCA 
    AGGATAATGG TGGCAGCAAA AAATAAAATC TTTCTTTTTG ACATTCATAC TAGTGGCCTA 
    TTGGGAGAAA TCCACACGGG CCATCACAGC ACCATCCAGT ACTGTGACTT CTCCCCACAA 
    AACCATTTGG CAGTGGTTGC TTTGTCCCAG TACTGTGTAG AGTTGTGGAA TACAGACTCA 
    CGTTCAAAGG TGGCTGATTG CAGAGGACAT TTAAGTTGGG TTCATGGTGT GATGTTTTCT 
    CCTGATGGAT CATCATTTTT GACATCTTCT GATGACCAGA CAATCAGGCT CTGGGAGACA 
    AAGAAAGTAT GTAAGAACTC TGCTGTAATG TTAAAGCAAG AAGTAGATGT TGTGTTTCAA 
    GAAAATGAAG TGATGGTCCT TGCAGTTGAC CATATAAGAC GTCTGCAACT CATTAATGGA 
    AGAACAGGTC AGATTGATTA TCTGACTGAA GCTCAAGTTA GCTGCTGTTG CTTAAGTCCA 
    CATCTTCAGT ACATTGCATT TGGAGATGAA AATGGAGCCA TTGAGATTTT AGAACTTGTA 
    AACAATAGAA TCTTCCAGTC CAGGTTTCAG CACAAGAAAA CTGTATGGCA CATCCAGTTC 
    ACAGCCGATG AGAAGACTCT TATTTCAAGT TCTGATGATG CTGAAATTCA GGTATGGAAT 
    TGGCAATTGG ACAAATGTAT CTTTCTACGA GGCCATCAGG AAACAGTGAA AGACTTTAGA 
    CTCTTGAAAA ATTCAAGACT GCTTTCTTGG TCATTTGATG GAACAGTGAA GGTATGGAAT 
    ATTATTACTG GAAATAAAGA AAAAGACTTT GTCTGTCACC AGGGTACAGT ACTTTCTTGT 
    GACATTTCTC ACGATGCTAC CAAGTTTTCA TCTACCTCTG CTGACAAGAC TGCAAAGATC 
    TGGAGTTTTG ATCTCCTTTT GCCACTTCAT GAATTGAGGG GCCACAACGG CTGTGTGCGC 
    TGCTCTGCCT TCTCTGTGGA CAGTACCCTG CTGGCAACGG GAGATGACAA TGGAGAAATC 
    AGGATATGGA ATGTCTCAAA CGGTGAGCTT CTTCATTTGT GTGCTCCGCT TTCAGAAGAA 
    GGAGCTGCTA CCCATGGAGG CTGGGTGACT GACCTTTGCT TTTCTCCAGA TGGCAAAATG 
    CTTATCTCTG CTGGAGGATA TATTAAGTGG TGGAACGTTG TCACTGGGGA ATCCTCACAG 
    ACCTTCTACA CAAATGGAAC CAATCTTAAG AAAATACACG TGTCCCCTGA CTTCAAAACA 
    TATGTGACTG TGGATAATCT TGGTATTTTA TATATTTTAC AGACTTTAGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_037361.1
    CDS578..4291
    Translation

    Target ORF information:

    RefSeq Version NM_013229.2
    Organism Homo sapiens(human)
    Definition Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_013229.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGTAAGG 
    ACAGTCCTGT GTGAAGGTGG AGTACCACAG AGGCCAGTTG TTTTTGTCAC AAGGAAGAAG 
    CTGGTGAATG CAATTCAGCA GAAGCTCTCC AAATTGAAAG GTGAACCAGG ATGGGTCACC 
    ATACATGGAA TGGCAGGCTG TGGGAAGTCT GTATTAGCTG CAGAAGCTGT TAGAGATCAT 
    TCCCTTTTAG AAGGTTGTTT CCCAGGGGGA GTGCATTGGG TTTCAGTTGG GAAACAAGAC 
    AAATCTGGGC TTCTGATGAA ACTGCAGAAT CTTTGCACAC GGTTGGATCA GGATGAGAGT 
    TTTTCCCAGA GGCTTCCACT TAATATTGAA GAGGCTAAAG ACCGTCTCCG CATTCTGATG 
    CTTCGCAAAC ACCCAAGGTC TCTCTTGATC TTGGATGATG TTTGGGACTC TTGGGTGTTG 
    AAAGCTTTTG ACAGTCAGTG TCAGATTCTT CTTACAACCA GAGACAAGAG TGTTACAGAT 
    TCAGTAATGG GTCCTAAATA TGTAGTCCCT GTGGAGAGTT CCTTAGGAAA GGAAAAAGGA 
    CTTGAAATTT TATCCCTTTT TGTTAATATG AAGAAGGCAG ATTTGCCAGA ACAAGCTCAT 
    AGTATTATAA AAGAATGTAA AGGCTCTCCC CTTGTAGTAT CTTTAATTGG TGCACTTTTA 
    CGTGATTTTC CCAATCGCTG GGAGTACTAC CTCAAACAGC TTCAGAATAA GCAGTTTAAG 
    AGAATAAGGA AATCTTCGTC TTATGATTAT GAGGCTCTAG ATGAAGCCAT GTCTATAAGT 
    GTTGAAATGC TCAGAGAAGA CATCAAAGAT TATTACACAG ATCTTTCCAT CCTTCAGAAG 
    GACGTTAAGG TGCCTACAAA GGTGTTATGT ATTCTCTGGG ACATGGAAAC TGAAGAAGTT 
    GAAGACATAC TGCAGGAGTT TGTAAATAAG TCTCTTTTAT TCTGTGATCG GAATGGAAAG 
    TCGTTTCGTT ATTATTTACA TGATCTTCAA GTAGATTTTC TTACAGAGAA GAATTGCAGC 
    CAGCTTCAGG ATCTACATAA GAAGATAATC ACTCAGTTTC AGAGATATCA CCAGCCGCAT 
    ACTCTTTCAC CAGATCAGGA AGACTGTATG TATTGGTACA ACTTTCTGGC CTATCACATG 
    GCCAGTGCCA AGATGCACAA GGAACTTTGT GCTTTAATGT TTTCCCTGGA TTGGATTAAA 
    GCAAAAACAG AACTTGTAGG CCCTGCTCAT CTGATTCATG AATTTGTGGA ATACAGACAT 
    ATACTAGATG AAAAGGATTG TGCAGTCAGT GAGAATTTTC AGGAGTTTTT ATCTTTAAAT 
    GGACACCTTC TTGGACGACA GCCATTTCCT AATATTGTAC AACTGGGTCT CTGTGAGCCG 
    GAAACTTCAG AAGTTTATCA GCAAGCTAAG CTGCAGGCCA AGCAGGAGGT CGATAATGGA 
    ATGCTTTACC TGGAATGGAT AAACAAAAAA AACATCACGA ATCTTTCCCG CTTAGTTGTC 
    CGCCCCCACA CAGATGCTGT TTACCATGCC TGCTTTTCTG AGGATGGTCA GAGAATAGCT 
    TCTTGTGGAG CTGATAAAAC CTTACAGGTG TTCAAAGCTG AAACAGGAGA GAAACTTCTA 
    GAAATCAAGG CTCATGAGGA TGAAGTGCTT TGTTGTGCAT TCTCTACAGA TGACAGATTT 
    ATAGCAACCT GCTCAGTGGA TAAAAAAGTG AAGATTTGGA ATTCTATGAC TGGGGAACTA 
    GTACACACCT ATGATGAGCA CTCAGAGCAA GTCAATTGCT GCCATTTCAC CAACAGTAGT 
    CATCATCTTC TCTTAGCCAC TGGGTCAAGT GACTGCTTCC TCAAACTTTG GGATTTGAAT 
    CAAAAAGAAT GTCGAAATAC CATGTTTGGT CATACAAATT CAGTCAATCA CTGCAGATTT 
    TCACCAGATG ATAAGCTTTT GGCTAGTTGT TCAGCTGATG GAACCTTAAA GCTTTGGGAT 
    GCGACATCAG CAAATGAGAG GAAAAGCATT AATGTGAAAC AGTTCTTCCT AAATTTGGAG 
    GACCCTCAAG AGGATATGGA AGTGATAGTG AAGTGTTGTT CGTGGTCTGC TGATGGTGCA 
    AGGATAATGG TGGCAGCAAA AAATAAAATC TTTCTTTTTG ACATTCATAC TAGTGGCCTA 
    TTGGGAGAAA TCCACACGGG CCATCACAGC ACCATCCAGT ACTGTGACTT CTCCCCACAA 
    AACCATTTGG CAGTGGTTGC TTTGTCCCAG TACTGTGTAG AGTTGTGGAA TACAGACTCA 
    CGTTCAAAGG TGGCTGATTG CAGAGGACAT TTAAGTTGGG TTCATGGTGT GATGTTTTCT 
    CCTGATGGAT CATCATTTTT GACATCTTCT GATGACCAGA CAATCAGGCT CTGGGAGACA 
    AAGAAAGTAT GTAAGAACTC TGCTGTAATG TTAAAGCAAG AAGTAGATGT TGTGTTTCAA 
    GAAAATGAAG TGATGGTCCT TGCAGTTGAC CATATAAGAC GTCTGCAACT CATTAATGGA 
    AGAACAGGTC AGATTGATTA TCTGACTGAA GCTCAAGTTA GCTGCTGTTG CTTAAGTCCA 
    CATCTTCAGT ACATTGCATT TGGAGATGAA AATGGAGCCA TTGAGATTTT AGAACTTGTA 
    AACAATAGAA TCTTCCAGTC CAGGTTTCAG CACAAGAAAA CTGTATGGCA CATCCAGTTC 
    ACAGCCGATG AGAAGACTCT TATTTCAAGT TCTGATGATG CTGAAATTCA GGTATGGAAT 
    TGGCAATTGG ACAAATGTAT CTTTCTACGA GGCCATCAGG AAACAGTGAA AGACTTTAGA 
    CTCTTGAAAA ATTCAAGACT GCTTTCTTGG TCATTTGATG GAACAGTGAA GGTATGGAAT 
    ATTATTACTG GAAATAAAGA AAAAGACTTT GTCTGTCACC AGGGTACAGT ACTTTCTTGT 
    GACATTTCTC ACGATGCTAC CAAGTTTTCA TCTACCTCTG CTGACAAGAC TGCAAAGATC 
    TGGAGTTTTG ATCTCCTTTT GCCACTTCAT GAATTGAGGG GCCACAACGG CTGTGTGCGC 
    TGCTCTGCCT TCTCTGTGGA CAGTACCCTG CTGGCAACGG GAGATGACAA TGGAGAAATC 
    AGGATATGGA ATGTCTCAAA CGGTGAGCTT CTTCATTTGT GTGCTCCGCT TTCAGAAGAA 
    GGAGCTGCTA CCCATGGAGG CTGGGTGACT GACCTTTGCT TTTCTCCAGA TGGCAAAATG 
    CTTATCTCTG CTGGAGGATA TATTAAGTGG TGGAACGTTG TCACTGGGGA ATCCTCACAG 
    ACCTTCTACA CAAATGGAAC CAATCTTAAG AAAATACACG TGTCCCCTGA CTTCAAAACA 
    TATGTGACTG TGGATAATCT TGGTATTTTA TATATTTTAC AGACTTTAGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    APAF1 ( NM_181868.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_863658.1 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 4, mRNA.
    Price & Availability↑
    CloneID OHu21003
    Clone ID Related Accession (Same CDS sequence) NM_181868.1
    Accession Version NM_181868.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3618bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product apoptotic protease-activating factor 1 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF013263.1, AJ243003.1 and AC011248.8. Transcript Variant: This variant (4), also known as Apaf-1M, lacks an in-frame exon in the coding region, compared to variant 3. Variant 4 encodes isoform d, which is shorter than isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: EF560718.1, AJ243003.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGGTAAA 
    GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
    CAGAGGCCAG TTGTTTTTGT CACAAGGAAG AAGCTGGTGA ATGCAATTCA GCAGAAGCTC 
    TCCAAATTGA AAGGTGAACC AGGATGGGTC ACCATACATG GAATGGCAGG CTGTGGGAAG 
    TCTGTATTAG CTGCAGAAGC TGTTAGAGAT CATTCCCTTT TAGAAGGTTG TTTCCCAGGG 
    GGAGTGCATT GGGTTTCAGT TGGGAAACAA GACAAATCTG GGCTTCTGAT GAAACTGCAG 
    AATCTTTGCA CACGGTTGGA TCAGGATGAG AGTTTTTCCC AGAGGCTTCC ACTTAATATT 
    GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTTCGCA AACACCCAAG GTCTCTCTTG 
    ATCTTGGATG ATGTTTGGGA CTCTTGGGTG TTGAAAGCTT TTGACAGTCA GTGTCAGATT 
    CTTCTTACAA CCAGAGACAA GAGTGTTACA GATTCAGTAA TGGGTCCTAA ATATGTAGTC 
    CCTGTGGAGA GTTCCTTAGG AAAGGAAAAA GGACTTGAAA TTTTATCCCT TTTTGTTAAT 
    ATGAAGAAGG CAGATTTGCC AGAACAAGCT CATAGTATTA TAAAAGAATG TAAAGGCTCT 
    CCCCTTGTAG TATCTTTAAT TGGTGCACTT TTACGTGATT TTCCCAATCG CTGGGAGTAC 
    TACCTCAAAC AGCTTCAGAA TAAGCAGTTT AAGAGAATAA GGAAATCTTC GTCTTATGAT 
    TATGAGGCTC TAGATGAAGC CATGTCTATA AGTGTTGAAA TGCTCAGAGA AGACATCAAA 
    GATTATTACA CAGATCTTTC CATCCTTCAG AAGGACGTTA AGGTGCCTAC AAAGGTGTTA 
    TGTATTCTCT GGGACATGGA AACTGAAGAA GTTGAAGACA TACTGCAGGA GTTTGTAAAT 
    AAGTCTCTTT TATTCTGTGA TCGGAATGGA AAGTCGTTTC GTTATTATTT ACATGATCTT 
    CAAGTAGATT TTCTTACAGA GAAGAATTGC AGCCAGCTTC AGGATCTACA TAAGAAGATA 
    ATCACTCAGT TTCAGAGATA TCACCAGCCG CATACTCTTT CACCAGATCA GGAAGACTGT 
    ATGTATTGGT ACAACTTTCT GGCCTATCAC ATGGCCAGTG CCAAGATGCA CAAGGAACTT 
    TGTGCTTTAA TGTTTTCCCT GGATTGGATT AAAGCAAAAA CAGAACTTGT AGGCCCTGCT 
    CATCTGATTC ATGAATTTGT GGAATACAGA CATATACTAG ATGAAAAGGA TTGTGCAGTC 
    AGTGAGAATT TTCAGGAGTT TTTATCTTTA AATGGACACC TTCTTGGACG ACAGCCATTT 
    CCTAATATTG TACAACTGGG TCTCTGTGAG CCGGAAACTT CAGAAGTTTA TCAGCAAGCT 
    AAGCTGCAGG CCAAGCAGGA GGTCGATAAT GGAATGCTTT ACCTGGAATG GATAAACAAA 
    AAAAACATCA CGAATCTTTC CCGCTTAGTT GTCCGCCCCC ACACAGATGC TGTTTACCAT 
    GCCTGCTTTT CTGAGGATGG TCAGAGAATA GCTTCTTGTG GAGCTGATAA AACCTTACAG 
    GTGTTCAAAG CTGAAACAGG AGAGAAACTT CTAGAAATCA AGGCTCATGA GGATGAAGTG 
    CTTTGTTGTG CATTCTCTAC AGATGACAGA TTTATAGCAA CCTGCTCAGT GGATAAAAAA 
    GTGAAGATTT GGAATTCTAT GACTGGGGAA CTAGTACACA CCTATGATGA GCACTCAGAG 
    CAAGTCAATT GCTGCCATTT CACCAACAGT AGTCATCATC TTCTCTTAGC CACTGGGTCA 
    AGTGACTGCT TCCTCAAACT TTGGGATTTG AATCAAAAAG AATGTCGAAA TACCATGTTT 
    GGTCATACAA ATTCAGTCAA TCACTGCAGA TTTTCACCAG ATGATAAGCT TTTGGCTAGT 
    TGTTCAGCTG ATGGAACCTT AAAGCTTTGG GATGCGACAT CAGCAAATGA GAGGAAAAGC 
    ATTAATGTGA AACAGTTCTT CCTAAATTTG GAGGACCCTC AAGAGGATAT GGAAGTGATA 
    GTGAAGTGTT GTTCGTGGTC TGCTGATGGT GCAAGGATAA TGGTGGCAGC AAAAAATAAA 
    ATCTTTTTGT GGAATACAGA CTCACGTTCA AAGGTGGCTG ATTGCAGAGG ACATTTAAGT 
    TGGGTTCATG GTGTGATGTT TTCTCCTGAT GGATCATCAT TTTTGACATC TTCTGATGAC 
    CAGACAATCA GGCTCTGGGA GACAAAGAAA GTATGTAAGA ACTCTGCTGT AATGTTAAAG 
    CAAGAAGTAG ATGTTGTGTT TCAAGAAAAT GAAGTGATGG TCCTTGCAGT TGACCATATA 
    AGACGTCTGC AACTCATTAA TGGAAGAACA GGTCAGATTG ATTATCTGAC TGAAGCTCAA 
    GTTAGCTGCT GTTGCTTAAG TCCACATCTT CAGTACATTG CATTTGGAGA TGAAAATGGA 
    GCCATTGAGA TTTTAGAACT TGTAAACAAT AGAATCTTCC AGTCCAGGTT TCAGCACAAG 
    AAAACTGTAT GGCACATCCA GTTCACAGCC GATGAGAAGA CTCTTATTTC AAGTTCTGAT 
    GATGCTGAAA TTCAGGTATG GAATTGGCAA TTGGACAAAT GTATCTTTCT ACGAGGCCAT 
    CAGGAAACAG TGAAAGACTT TAGACTCTTG AAAAATTCAA GACTGCTTTC TTGGTCATTT 
    GATGGAACAG TGAAGGTATG GAATATTATT ACTGGAAATA AAGAAAAAGA CTTTGTCTGT 
    CACCAGGGTA CAGTACTTTC TTGTGACATT TCTCACGATG CTACCAAGTT TTCATCTACC 
    TCTGCTGACA AGACTGCAAA GATCTGGAGT TTTGATCTCC TTTTGCCACT TCATGAATTG 
    AGGGGCCACA ACGGCTGTGT GCGCTGCTCT GCCTTCTCTG TGGACAGTAC CCTGCTGGCA 
    ACGGGAGATG ACAATGGAGA AATCAGGATA TGGAATGTCT CAAACGGTGA GCTTCTTCAT 
    TTGTGTGCTC CGCTTTCAGA AGAAGGAGCT GCTACCCATG GAGGCTGGGT GACTGACCTT 
    TGCTTTTCTC CAGATGGCAA AATGCTTATC TCTGCTGGAG GATATATTAA GTGGTGGAAC 
    GTTGTCACTG GGGAATCCTC ACAGACCTTC TACACAAATG GAACCAATCT TAAGAAAATA 
    CACGTGTCCC CTGACTTCAA AACATATGTG ACTGTGGATA ATCTTGGTAT TTTATATATT 
    TTACAGACTT TAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_863658.1
    CDS578..4195
    Translation

    Target ORF information:

    RefSeq Version NM_181868.1
    Organism Homo sapiens(human)
    Definition Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_181868.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGGTAAA 
    GATTCAGTTA GTGGAATAAC TTCGTATGTA AGGACAGTCC TGTGTGAAGG TGGAGTACCA 
    CAGAGGCCAG TTGTTTTTGT CACAAGGAAG AAGCTGGTGA ATGCAATTCA GCAGAAGCTC 
    TCCAAATTGA AAGGTGAACC AGGATGGGTC ACCATACATG GAATGGCAGG CTGTGGGAAG 
    TCTGTATTAG CTGCAGAAGC TGTTAGAGAT CATTCCCTTT TAGAAGGTTG TTTCCCAGGG 
    GGAGTGCATT GGGTTTCAGT TGGGAAACAA GACAAATCTG GGCTTCTGAT GAAACTGCAG 
    AATCTTTGCA CACGGTTGGA TCAGGATGAG AGTTTTTCCC AGAGGCTTCC ACTTAATATT 
    GAAGAGGCTA AAGACCGTCT CCGCATTCTG ATGCTTCGCA AACACCCAAG GTCTCTCTTG 
    ATCTTGGATG ATGTTTGGGA CTCTTGGGTG TTGAAAGCTT TTGACAGTCA GTGTCAGATT 
    CTTCTTACAA CCAGAGACAA GAGTGTTACA GATTCAGTAA TGGGTCCTAA ATATGTAGTC 
    CCTGTGGAGA GTTCCTTAGG AAAGGAAAAA GGACTTGAAA TTTTATCCCT TTTTGTTAAT 
    ATGAAGAAGG CAGATTTGCC AGAACAAGCT CATAGTATTA TAAAAGAATG TAAAGGCTCT 
    CCCCTTGTAG TATCTTTAAT TGGTGCACTT TTACGTGATT TTCCCAATCG CTGGGAGTAC 
    TACCTCAAAC AGCTTCAGAA TAAGCAGTTT AAGAGAATAA GGAAATCTTC GTCTTATGAT 
    TATGAGGCTC TAGATGAAGC CATGTCTATA AGTGTTGAAA TGCTCAGAGA AGACATCAAA 
    GATTATTACA CAGATCTTTC CATCCTTCAG AAGGACGTTA AGGTGCCTAC AAAGGTGTTA 
    TGTATTCTCT GGGACATGGA AACTGAAGAA GTTGAAGACA TACTGCAGGA GTTTGTAAAT 
    AAGTCTCTTT TATTCTGTGA TCGGAATGGA AAGTCGTTTC GTTATTATTT ACATGATCTT 
    CAAGTAGATT TTCTTACAGA GAAGAATTGC AGCCAGCTTC AGGATCTACA TAAGAAGATA 
    ATCACTCAGT TTCAGAGATA TCACCAGCCG CATACTCTTT CACCAGATCA GGAAGACTGT 
    ATGTATTGGT ACAACTTTCT GGCCTATCAC ATGGCCAGTG CCAAGATGCA CAAGGAACTT 
    TGTGCTTTAA TGTTTTCCCT GGATTGGATT AAAGCAAAAA CAGAACTTGT AGGCCCTGCT 
    CATCTGATTC ATGAATTTGT GGAATACAGA CATATACTAG ATGAAAAGGA TTGTGCAGTC 
    AGTGAGAATT TTCAGGAGTT TTTATCTTTA AATGGACACC TTCTTGGACG ACAGCCATTT 
    CCTAATATTG TACAACTGGG TCTCTGTGAG CCGGAAACTT CAGAAGTTTA TCAGCAAGCT 
    AAGCTGCAGG CCAAGCAGGA GGTCGATAAT GGAATGCTTT ACCTGGAATG GATAAACAAA 
    AAAAACATCA CGAATCTTTC CCGCTTAGTT GTCCGCCCCC ACACAGATGC TGTTTACCAT 
    GCCTGCTTTT CTGAGGATGG TCAGAGAATA GCTTCTTGTG GAGCTGATAA AACCTTACAG 
    GTGTTCAAAG CTGAAACAGG AGAGAAACTT CTAGAAATCA AGGCTCATGA GGATGAAGTG 
    CTTTGTTGTG CATTCTCTAC AGATGACAGA TTTATAGCAA CCTGCTCAGT GGATAAAAAA 
    GTGAAGATTT GGAATTCTAT GACTGGGGAA CTAGTACACA CCTATGATGA GCACTCAGAG 
    CAAGTCAATT GCTGCCATTT CACCAACAGT AGTCATCATC TTCTCTTAGC CACTGGGTCA 
    AGTGACTGCT TCCTCAAACT TTGGGATTTG AATCAAAAAG AATGTCGAAA TACCATGTTT 
    GGTCATACAA ATTCAGTCAA TCACTGCAGA TTTTCACCAG ATGATAAGCT TTTGGCTAGT 
    TGTTCAGCTG ATGGAACCTT AAAGCTTTGG GATGCGACAT CAGCAAATGA GAGGAAAAGC 
    ATTAATGTGA AACAGTTCTT CCTAAATTTG GAGGACCCTC AAGAGGATAT GGAAGTGATA 
    GTGAAGTGTT GTTCGTGGTC TGCTGATGGT GCAAGGATAA TGGTGGCAGC AAAAAATAAA 
    ATCTTTTTGT GGAATACAGA CTCACGTTCA AAGGTGGCTG ATTGCAGAGG ACATTTAAGT 
    TGGGTTCATG GTGTGATGTT TTCTCCTGAT GGATCATCAT TTTTGACATC TTCTGATGAC 
    CAGACAATCA GGCTCTGGGA GACAAAGAAA GTATGTAAGA ACTCTGCTGT AATGTTAAAG 
    CAAGAAGTAG ATGTTGTGTT TCAAGAAAAT GAAGTGATGG TCCTTGCAGT TGACCATATA 
    AGACGTCTGC AACTCATTAA TGGAAGAACA GGTCAGATTG ATTATCTGAC TGAAGCTCAA 
    GTTAGCTGCT GTTGCTTAAG TCCACATCTT CAGTACATTG CATTTGGAGA TGAAAATGGA 
    GCCATTGAGA TTTTAGAACT TGTAAACAAT AGAATCTTCC AGTCCAGGTT TCAGCACAAG 
    AAAACTGTAT GGCACATCCA GTTCACAGCC GATGAGAAGA CTCTTATTTC AAGTTCTGAT 
    GATGCTGAAA TTCAGGTATG GAATTGGCAA TTGGACAAAT GTATCTTTCT ACGAGGCCAT 
    CAGGAAACAG TGAAAGACTT TAGACTCTTG AAAAATTCAA GACTGCTTTC TTGGTCATTT 
    GATGGAACAG TGAAGGTATG GAATATTATT ACTGGAAATA AAGAAAAAGA CTTTGTCTGT 
    CACCAGGGTA CAGTACTTTC TTGTGACATT TCTCACGATG CTACCAAGTT TTCATCTACC 
    TCTGCTGACA AGACTGCAAA GATCTGGAGT TTTGATCTCC TTTTGCCACT TCATGAATTG 
    AGGGGCCACA ACGGCTGTGT GCGCTGCTCT GCCTTCTCTG TGGACAGTAC CCTGCTGGCA 
    ACGGGAGATG ACAATGGAGA AATCAGGATA TGGAATGTCT CAAACGGTGA GCTTCTTCAT 
    TTGTGTGCTC CGCTTTCAGA AGAAGGAGCT GCTACCCATG GAGGCTGGGT GACTGACCTT 
    TGCTTTTCTC CAGATGGCAA AATGCTTATC TCTGCTGGAG GATATATTAA GTGGTGGAAC 
    GTTGTCACTG GGGAATCCTC ACAGACCTTC TACACAAATG GAACCAATCT TAAGAAAATA 
    CACGTGTCCC CTGACTTCAA AACATATGTG ACTGTGGATA ATCTTGGTAT TTTATATATT 
    TTACAGACTT TAGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    APAF1 ( NM_001160.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001151.1 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu21771
    Clone ID Related Accession (Same CDS sequence) NM_001160.2 , NM_001160.3
    Accession Version NM_001160.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-18
    Organism Homo sapiens(human)
    Product apoptotic protease-activating factor 1 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF013263.1 and AC011248.8. On Jul 9, 2003 this sequence version replaced NM_001160.1. Transcript Variant: This variant (2) uses an alternate splice site and lacks an exon in the coding region but maintains the reading frame, compared to variant 3. Variant 2 encodes isoform b, which is shorter than isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF013263.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGTAAGG 
    ACAGTCCTGT GTGAAGGTGG AGTACCACAG AGGCCAGTTG TTTTTGTCAC AAGGAAGAAG 
    CTGGTGAATG CAATTCAGCA GAAGCTCTCC AAATTGAAAG GTGAACCAGG ATGGGTCACC 
    ATACATGGAA TGGCAGGCTG TGGGAAGTCT GTATTAGCTG CAGAAGCTGT TAGAGATCAT 
    TCCCTTTTAG AAGGTTGTTT CCCAGGGGGA GTGCATTGGG TTTCAGTTGG GAAACAAGAC 
    AAATCTGGGC TTCTGATGAA ACTGCAGAAT CTTTGCACAC GGTTGGATCA GGATGAGAGT 
    TTTTCCCAGA GGCTTCCACT TAATATTGAA GAGGCTAAAG ACCGTCTCCG CATTCTGATG 
    CTTCGCAAAC ACCCAAGGTC TCTCTTGATC TTGGATGATG TTTGGGACTC TTGGGTGTTG 
    AAAGCTTTTG ACAGTCAGTG TCAGATTCTT CTTACAACCA GAGACAAGAG TGTTACAGAT 
    TCAGTAATGG GTCCTAAATA TGTAGTCCCT GTGGAGAGTT CCTTAGGAAA GGAAAAAGGA 
    CTTGAAATTT TATCCCTTTT TGTTAATATG AAGAAGGCAG ATTTGCCAGA ACAAGCTCAT 
    AGTATTATAA AAGAATGTAA AGGCTCTCCC CTTGTAGTAT CTTTAATTGG TGCACTTTTA 
    CGTGATTTTC CCAATCGCTG GGAGTACTAC CTCAAACAGC TTCAGAATAA GCAGTTTAAG 
    AGAATAAGGA AATCTTCGTC TTATGATTAT GAGGCTCTAG ATGAAGCCAT GTCTATAAGT 
    GTTGAAATGC TCAGAGAAGA CATCAAAGAT TATTACACAG ATCTTTCCAT CCTTCAGAAG 
    GACGTTAAGG TGCCTACAAA GGTGTTATGT ATTCTCTGGG ACATGGAAAC TGAAGAAGTT 
    GAAGACATAC TGCAGGAGTT TGTAAATAAG TCTCTTTTAT TCTGTGATCG GAATGGAAAG 
    TCGTTTCGTT ATTATTTACA TGATCTTCAA GTAGATTTTC TTACAGAGAA GAATTGCAGC 
    CAGCTTCAGG ATCTACATAA GAAGATAATC ACTCAGTTTC AGAGATATCA CCAGCCGCAT 
    ACTCTTTCAC CAGATCAGGA AGACTGTATG TATTGGTACA ACTTTCTGGC CTATCACATG 
    GCCAGTGCCA AGATGCACAA GGAACTTTGT GCTTTAATGT TTTCCCTGGA TTGGATTAAA 
    GCAAAAACAG AACTTGTAGG CCCTGCTCAT CTGATTCATG AATTTGTGGA ATACAGACAT 
    ATACTAGATG AAAAGGATTG TGCAGTCAGT GAGAATTTTC AGGAGTTTTT ATCTTTAAAT 
    GGACACCTTC TTGGACGACA GCCATTTCCT AATATTGTAC AACTGGGTCT CTGTGAGCCG 
    GAAACTTCAG AAGTTTATCA GCAAGCTAAG CTGCAGGCCA AGCAGGAGGT CGATAATGGA 
    ATGCTTTACC TGGAATGGAT AAACAAAAAA AACATCACGA ATCTTTCCCG CTTAGTTGTC 
    CGCCCCCACA CAGATGCTGT TTACCATGCC TGCTTTTCTG AGGATGGTCA GAGAATAGCT 
    TCTTGTGGAG CTGATAAAAC CTTACAGGTG TTCAAAGCTG AAACAGGAGA GAAACTTCTA 
    GAAATCAAGG CTCATGAGGA TGAAGTGCTT TGTTGTGCAT TCTCTACAGA TGACAGATTT 
    ATAGCAACCT GCTCAGTGGA TAAAAAAGTG AAGATTTGGA ATTCTATGAC TGGGGAACTA 
    GTACACACCT ATGATGAGCA CTCAGAGCAA GTCAATTGCT GCCATTTCAC CAACAGTAGT 
    CATCATCTTC TCTTAGCCAC TGGGTCAAGT GACTGCTTCC TCAAACTTTG GGATTTGAAT 
    CAAAAAGAAT GTCGAAATAC CATGTTTGGT CATACAAATT CAGTCAATCA CTGCAGATTT 
    TCACCAGATG ATAAGCTTTT GGCTAGTTGT TCAGCTGATG GAACCTTAAA GCTTTGGGAT 
    GCGACATCAG CAAATGAGAG GAAAAGCATT AATGTGAAAC AGTTCTTCCT AAATTTGGAG 
    GACCCTCAAG AGGATATGGA AGTGATAGTG AAGTGTTGTT CGTGGTCTGC TGATGGTGCA 
    AGGATAATGG TGGCAGCAAA AAATAAAATC TTTTTGTGGA ATACAGACTC ACGTTCAAAG 
    GTGGCTGATT GCAGAGGACA TTTAAGTTGG GTTCATGGTG TGATGTTTTC TCCTGATGGA 
    TCATCATTTT TGACATCTTC TGATGACCAG ACAATCAGGC TCTGGGAGAC AAAGAAAGTA 
    TGTAAGAACT CTGCTGTAAT GTTAAAGCAA GAAGTAGATG TTGTGTTTCA AGAAAATGAA 
    GTGATGGTCC TTGCAGTTGA CCATATAAGA CGTCTGCAAC TCATTAATGG AAGAACAGGT 
    CAGATTGATT ATCTGACTGA AGCTCAAGTT AGCTGCTGTT GCTTAAGTCC ACATCTTCAG 
    TACATTGCAT TTGGAGATGA AAATGGAGCC ATTGAGATTT TAGAACTTGT AAACAATAGA 
    ATCTTCCAGT CCAGGTTTCA GCACAAGAAA ACTGTATGGC ACATCCAGTT CACAGCCGAT 
    GAGAAGACTC TTATTTCAAG TTCTGATGAT GCTGAAATTC AGGTATGGAA TTGGCAATTG 
    GACAAATGTA TCTTTCTACG AGGCCATCAG GAAACAGTGA AAGACTTTAG ACTCTTGAAA 
    AATTCAAGAC TGCTTTCTTG GTCATTTGAT GGAACAGTGA AGGTATGGAA TATTATTACT 
    GGAAATAAAG AAAAAGACTT TGTCTGTCAC CAGGGTACAG TACTTTCTTG TGACATTTCT 
    CACGATGCTA CCAAGTTTTC ATCTACCTCT GCTGACAAGA CTGCAAAGAT CTGGAGTTTT 
    GATCTCCTTT TGCCACTTCA TGAATTGAGG GGCCACAACG GCTGTGTGCG CTGCTCTGCC 
    TTCTCTGTGG ACAGTACCCT GCTGGCAACG GGAGATGACA ATGGAGAAAT CAGGATATGG 
    AATGTCTCAA ACGGTGAGCT TCTTCATTTG TGTGCTCCGC TTTCAGAAGA AGGAGCTGCT 
    ACCCATGGAG GCTGGGTGAC TGACCTTTGC TTTTCTCCAG ATGGCAAAAT GCTTATCTCT 
    GCTGGAGGAT ATATTAAGTG GTGGAACGTT GTCACTGGGG AATCCTCACA GACCTTCTAC 
    ACAAATGGAA CCAATCTTAA GAAAATACAC GTGTCCCCTG ACTTCAAAAC ATATGTGACT 
    GTGGATAATC TTGGTATTTT ATATATTTTA CAGACTTTAG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001151.1
    CDS578..4162
    Translation

    Target ORF information:

    RefSeq Version NM_001160.2
    Organism Homo sapiens(human)
    Definition Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001160.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGTAAGG 
    ACAGTCCTGT GTGAAGGTGG AGTACCACAG AGGCCAGTTG TTTTTGTCAC AAGGAAGAAG 
    CTGGTGAATG CAATTCAGCA GAAGCTCTCC AAATTGAAAG GTGAACCAGG ATGGGTCACC 
    ATACATGGAA TGGCAGGCTG TGGGAAGTCT GTATTAGCTG CAGAAGCTGT TAGAGATCAT 
    TCCCTTTTAG AAGGTTGTTT CCCAGGGGGA GTGCATTGGG TTTCAGTTGG GAAACAAGAC 
    AAATCTGGGC TTCTGATGAA ACTGCAGAAT CTTTGCACAC GGTTGGATCA GGATGAGAGT 
    TTTTCCCAGA GGCTTCCACT TAATATTGAA GAGGCTAAAG ACCGTCTCCG CATTCTGATG 
    CTTCGCAAAC ACCCAAGGTC TCTCTTGATC TTGGATGATG TTTGGGACTC TTGGGTGTTG 
    AAAGCTTTTG ACAGTCAGTG TCAGATTCTT CTTACAACCA GAGACAAGAG TGTTACAGAT 
    TCAGTAATGG GTCCTAAATA TGTAGTCCCT GTGGAGAGTT CCTTAGGAAA GGAAAAAGGA 
    CTTGAAATTT TATCCCTTTT TGTTAATATG AAGAAGGCAG ATTTGCCAGA ACAAGCTCAT 
    AGTATTATAA AAGAATGTAA AGGCTCTCCC CTTGTAGTAT CTTTAATTGG TGCACTTTTA 
    CGTGATTTTC CCAATCGCTG GGAGTACTAC CTCAAACAGC TTCAGAATAA GCAGTTTAAG 
    AGAATAAGGA AATCTTCGTC TTATGATTAT GAGGCTCTAG ATGAAGCCAT GTCTATAAGT 
    GTTGAAATGC TCAGAGAAGA CATCAAAGAT TATTACACAG ATCTTTCCAT CCTTCAGAAG 
    GACGTTAAGG TGCCTACAAA GGTGTTATGT ATTCTCTGGG ACATGGAAAC TGAAGAAGTT 
    GAAGACATAC TGCAGGAGTT TGTAAATAAG TCTCTTTTAT TCTGTGATCG GAATGGAAAG 
    TCGTTTCGTT ATTATTTACA TGATCTTCAA GTAGATTTTC TTACAGAGAA GAATTGCAGC 
    CAGCTTCAGG ATCTACATAA GAAGATAATC ACTCAGTTTC AGAGATATCA CCAGCCGCAT 
    ACTCTTTCAC CAGATCAGGA AGACTGTATG TATTGGTACA ACTTTCTGGC CTATCACATG 
    GCCAGTGCCA AGATGCACAA GGAACTTTGT GCTTTAATGT TTTCCCTGGA TTGGATTAAA 
    GCAAAAACAG AACTTGTAGG CCCTGCTCAT CTGATTCATG AATTTGTGGA ATACAGACAT 
    ATACTAGATG AAAAGGATTG TGCAGTCAGT GAGAATTTTC AGGAGTTTTT ATCTTTAAAT 
    GGACACCTTC TTGGACGACA GCCATTTCCT AATATTGTAC AACTGGGTCT CTGTGAGCCG 
    GAAACTTCAG AAGTTTATCA GCAAGCTAAG CTGCAGGCCA AGCAGGAGGT CGATAATGGA 
    ATGCTTTACC TGGAATGGAT AAACAAAAAA AACATCACGA ATCTTTCCCG CTTAGTTGTC 
    CGCCCCCACA CAGATGCTGT TTACCATGCC TGCTTTTCTG AGGATGGTCA GAGAATAGCT 
    TCTTGTGGAG CTGATAAAAC CTTACAGGTG TTCAAAGCTG AAACAGGAGA GAAACTTCTA 
    GAAATCAAGG CTCATGAGGA TGAAGTGCTT TGTTGTGCAT TCTCTACAGA TGACAGATTT 
    ATAGCAACCT GCTCAGTGGA TAAAAAAGTG AAGATTTGGA ATTCTATGAC TGGGGAACTA 
    GTACACACCT ATGATGAGCA CTCAGAGCAA GTCAATTGCT GCCATTTCAC CAACAGTAGT 
    CATCATCTTC TCTTAGCCAC TGGGTCAAGT GACTGCTTCC TCAAACTTTG GGATTTGAAT 
    CAAAAAGAAT GTCGAAATAC CATGTTTGGT CATACAAATT CAGTCAATCA CTGCAGATTT 
    TCACCAGATG ATAAGCTTTT GGCTAGTTGT TCAGCTGATG GAACCTTAAA GCTTTGGGAT 
    GCGACATCAG CAAATGAGAG GAAAAGCATT AATGTGAAAC AGTTCTTCCT AAATTTGGAG 
    GACCCTCAAG AGGATATGGA AGTGATAGTG AAGTGTTGTT CGTGGTCTGC TGATGGTGCA 
    AGGATAATGG TGGCAGCAAA AAATAAAATC TTTTTGTGGA ATACAGACTC ACGTTCAAAG 
    GTGGCTGATT GCAGAGGACA TTTAAGTTGG GTTCATGGTG TGATGTTTTC TCCTGATGGA 
    TCATCATTTT TGACATCTTC TGATGACCAG ACAATCAGGC TCTGGGAGAC AAAGAAAGTA 
    TGTAAGAACT CTGCTGTAAT GTTAAAGCAA GAAGTAGATG TTGTGTTTCA AGAAAATGAA 
    GTGATGGTCC TTGCAGTTGA CCATATAAGA CGTCTGCAAC TCATTAATGG AAGAACAGGT 
    CAGATTGATT ATCTGACTGA AGCTCAAGTT AGCTGCTGTT GCTTAAGTCC ACATCTTCAG 
    TACATTGCAT TTGGAGATGA AAATGGAGCC ATTGAGATTT TAGAACTTGT AAACAATAGA 
    ATCTTCCAGT CCAGGTTTCA GCACAAGAAA ACTGTATGGC ACATCCAGTT CACAGCCGAT 
    GAGAAGACTC TTATTTCAAG TTCTGATGAT GCTGAAATTC AGGTATGGAA TTGGCAATTG 
    GACAAATGTA TCTTTCTACG AGGCCATCAG GAAACAGTGA AAGACTTTAG ACTCTTGAAA 
    AATTCAAGAC TGCTTTCTTG GTCATTTGAT GGAACAGTGA AGGTATGGAA TATTATTACT 
    GGAAATAAAG AAAAAGACTT TGTCTGTCAC CAGGGTACAG TACTTTCTTG TGACATTTCT 
    CACGATGCTA CCAAGTTTTC ATCTACCTCT GCTGACAAGA CTGCAAAGAT CTGGAGTTTT 
    GATCTCCTTT TGCCACTTCA TGAATTGAGG GGCCACAACG GCTGTGTGCG CTGCTCTGCC 
    TTCTCTGTGG ACAGTACCCT GCTGGCAACG GGAGATGACA ATGGAGAAAT CAGGATATGG 
    AATGTCTCAA ACGGTGAGCT TCTTCATTTG TGTGCTCCGC TTTCAGAAGA AGGAGCTGCT 
    ACCCATGGAG GCTGGGTGAC TGACCTTTGC TTTTCTCCAG ATGGCAAAAT GCTTATCTCT 
    GCTGGAGGAT ATATTAAGTG GTGGAACGTT GTCACTGGGG AATCCTCACA GACCTTCTAC 
    ACAAATGGAA CCAATCTTAA GAAAATACAC GTGTCCCCTG ACTTCAAAAC ATATGTGACT 
    GTGGATAATC TTGGTATTTT ATATATTTTA CAGACTTTAG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    APAF1 ( NM_001160.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001151.1 Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 2, mRNA.
    Price & Availability↑
    CloneID OHu21771
    Clone ID Related Accession (Same CDS sequence) NM_001160.2 , NM_001160.3
    Accession Version NM_001160.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3585bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product apoptotic protease-activating factor 1 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC013283.23, AF013263.1 and AC011248.8. On Jun 2, 2019 this sequence version replaced NM_001160.2. Transcript Variant: This variant (2) uses an alternate splice site and lacks an exon in the coding region but maintains the reading frame, compared to variant 3. Variant 2 encodes isoform b, which is shorter than isoform c. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF013263.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGTAAGG 
    ACAGTCCTGT GTGAAGGTGG AGTACCACAG AGGCCAGTTG TTTTTGTCAC AAGGAAGAAG 
    CTGGTGAATG CAATTCAGCA GAAGCTCTCC AAATTGAAAG GTGAACCAGG ATGGGTCACC 
    ATACATGGAA TGGCAGGCTG TGGGAAGTCT GTATTAGCTG CAGAAGCTGT TAGAGATCAT 
    TCCCTTTTAG AAGGTTGTTT CCCAGGGGGA GTGCATTGGG TTTCAGTTGG GAAACAAGAC 
    AAATCTGGGC TTCTGATGAA ACTGCAGAAT CTTTGCACAC GGTTGGATCA GGATGAGAGT 
    TTTTCCCAGA GGCTTCCACT TAATATTGAA GAGGCTAAAG ACCGTCTCCG CATTCTGATG 
    CTTCGCAAAC ACCCAAGGTC TCTCTTGATC TTGGATGATG TTTGGGACTC TTGGGTGTTG 
    AAAGCTTTTG ACAGTCAGTG TCAGATTCTT CTTACAACCA GAGACAAGAG TGTTACAGAT 
    TCAGTAATGG GTCCTAAATA TGTAGTCCCT GTGGAGAGTT CCTTAGGAAA GGAAAAAGGA 
    CTTGAAATTT TATCCCTTTT TGTTAATATG AAGAAGGCAG ATTTGCCAGA ACAAGCTCAT 
    AGTATTATAA AAGAATGTAA AGGCTCTCCC CTTGTAGTAT CTTTAATTGG TGCACTTTTA 
    CGTGATTTTC CCAATCGCTG GGAGTACTAC CTCAAACAGC TTCAGAATAA GCAGTTTAAG 
    AGAATAAGGA AATCTTCGTC TTATGATTAT GAGGCTCTAG ATGAAGCCAT GTCTATAAGT 
    GTTGAAATGC TCAGAGAAGA CATCAAAGAT TATTACACAG ATCTTTCCAT CCTTCAGAAG 
    GACGTTAAGG TGCCTACAAA GGTGTTATGT ATTCTCTGGG ACATGGAAAC TGAAGAAGTT 
    GAAGACATAC TGCAGGAGTT TGTAAATAAG TCTCTTTTAT TCTGTGATCG GAATGGAAAG 
    TCGTTTCGTT ATTATTTACA TGATCTTCAA GTAGATTTTC TTACAGAGAA GAATTGCAGC 
    CAGCTTCAGG ATCTACATAA GAAGATAATC ACTCAGTTTC AGAGATATCA CCAGCCGCAT 
    ACTCTTTCAC CAGATCAGGA AGACTGTATG TATTGGTACA ACTTTCTGGC CTATCACATG 
    GCCAGTGCCA AGATGCACAA GGAACTTTGT GCTTTAATGT TTTCCCTGGA TTGGATTAAA 
    GCAAAAACAG AACTTGTAGG CCCTGCTCAT CTGATTCATG AATTTGTGGA ATACAGACAT 
    ATACTAGATG AAAAGGATTG TGCAGTCAGT GAGAATTTTC AGGAGTTTTT ATCTTTAAAT 
    GGACACCTTC TTGGACGACA GCCATTTCCT AATATTGTAC AACTGGGTCT CTGTGAGCCG 
    GAAACTTCAG AAGTTTATCA GCAAGCTAAG CTGCAGGCCA AGCAGGAGGT CGATAATGGA 
    ATGCTTTACC TGGAATGGAT AAACAAAAAA AACATCACGA ATCTTTCCCG CTTAGTTGTC 
    CGCCCCCACA CAGATGCTGT TTACCATGCC TGCTTTTCTG AGGATGGTCA GAGAATAGCT 
    TCTTGTGGAG CTGATAAAAC CTTACAGGTG TTCAAAGCTG AAACAGGAGA GAAACTTCTA 
    GAAATCAAGG CTCATGAGGA TGAAGTGCTT TGTTGTGCAT TCTCTACAGA TGACAGATTT 
    ATAGCAACCT GCTCAGTGGA TAAAAAAGTG AAGATTTGGA ATTCTATGAC TGGGGAACTA 
    GTACACACCT ATGATGAGCA CTCAGAGCAA GTCAATTGCT GCCATTTCAC CAACAGTAGT 
    CATCATCTTC TCTTAGCCAC TGGGTCAAGT GACTGCTTCC TCAAACTTTG GGATTTGAAT 
    CAAAAAGAAT GTCGAAATAC CATGTTTGGT CATACAAATT CAGTCAATCA CTGCAGATTT 
    TCACCAGATG ATAAGCTTTT GGCTAGTTGT TCAGCTGATG GAACCTTAAA GCTTTGGGAT 
    GCGACATCAG CAAATGAGAG GAAAAGCATT AATGTGAAAC AGTTCTTCCT AAATTTGGAG 
    GACCCTCAAG AGGATATGGA AGTGATAGTG AAGTGTTGTT CGTGGTCTGC TGATGGTGCA 
    AGGATAATGG TGGCAGCAAA AAATAAAATC TTTTTGTGGA ATACAGACTC ACGTTCAAAG 
    GTGGCTGATT GCAGAGGACA TTTAAGTTGG GTTCATGGTG TGATGTTTTC TCCTGATGGA 
    TCATCATTTT TGACATCTTC TGATGACCAG ACAATCAGGC TCTGGGAGAC AAAGAAAGTA 
    TGTAAGAACT CTGCTGTAAT GTTAAAGCAA GAAGTAGATG TTGTGTTTCA AGAAAATGAA 
    GTGATGGTCC TTGCAGTTGA CCATATAAGA CGTCTGCAAC TCATTAATGG AAGAACAGGT 
    CAGATTGATT ATCTGACTGA AGCTCAAGTT AGCTGCTGTT GCTTAAGTCC ACATCTTCAG 
    TACATTGCAT TTGGAGATGA AAATGGAGCC ATTGAGATTT TAGAACTTGT AAACAATAGA 
    ATCTTCCAGT CCAGGTTTCA GCACAAGAAA ACTGTATGGC ACATCCAGTT CACAGCCGAT 
    GAGAAGACTC TTATTTCAAG TTCTGATGAT GCTGAAATTC AGGTATGGAA TTGGCAATTG 
    GACAAATGTA TCTTTCTACG AGGCCATCAG GAAACAGTGA AAGACTTTAG ACTCTTGAAA 
    AATTCAAGAC TGCTTTCTTG GTCATTTGAT GGAACAGTGA AGGTATGGAA TATTATTACT 
    GGAAATAAAG AAAAAGACTT TGTCTGTCAC CAGGGTACAG TACTTTCTTG TGACATTTCT 
    CACGATGCTA CCAAGTTTTC ATCTACCTCT GCTGACAAGA CTGCAAAGAT CTGGAGTTTT 
    GATCTCCTTT TGCCACTTCA TGAATTGAGG GGCCACAACG GCTGTGTGCG CTGCTCTGCC 
    TTCTCTGTGG ACAGTACCCT GCTGGCAACG GGAGATGACA ATGGAGAAAT CAGGATATGG 
    AATGTCTCAA ACGGTGAGCT TCTTCATTTG TGTGCTCCGC TTTCAGAAGA AGGAGCTGCT 
    ACCCATGGAG GCTGGGTGAC TGACCTTTGC TTTTCTCCAG ATGGCAAAAT GCTTATCTCT 
    GCTGGAGGAT ATATTAAGTG GTGGAACGTT GTCACTGGGG AATCCTCACA GACCTTCTAC 
    ACAAATGGAA CCAATCTTAA GAAAATACAC GTGTCCCCTG ACTTCAAAAC ATATGTGACT 
    GTGGATAATC TTGGTATTTT ATATATTTTA CAGACTTTAG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001151.1
    CDS588..4172
    Translation

    Target ORF information:

    RefSeq Version NM_001160.3
    Organism Homo sapiens(human)
    Definition Homo sapiens apoptotic peptidase activating factor 1 (APAF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001160.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATGCAA AAGCTCGAAA TTGTTTGCTT CAACATAGAG AAGCTCTGGA AAAGGACATC 
    AAGACATCCT ACATCATGGA TCACATGATT AGTGATGGAT TTTTAACAAT ATCAGAAGAG 
    GAAAAAGTAA GAAATGAGCC CACTCAACAG CAAAGAGCAG CTATGCTGAT TAAAATGATA 
    CTTAAAAAAG ATAATGATTC CTACGTATCA TTCTACAATG CTCTACTACA TGAAGGATAT 
    AAAGATCTTG CTGCCCTTCT CCATGATGGC ATTCCTGTTG TCTCTTCTTC CAGTGTAAGG 
    ACAGTCCTGT GTGAAGGTGG AGTACCACAG AGGCCAGTTG TTTTTGTCAC AAGGAAGAAG 
    CTGGTGAATG CAATTCAGCA GAAGCTCTCC AAATTGAAAG GTGAACCAGG ATGGGTCACC 
    ATACATGGAA TGGCAGGCTG TGGGAAGTCT GTATTAGCTG CAGAAGCTGT TAGAGATCAT 
    TCCCTTTTAG AAGGTTGTTT CCCAGGGGGA GTGCATTGGG TTTCAGTTGG GAAACAAGAC 
    AAATCTGGGC TTCTGATGAA ACTGCAGAAT CTTTGCACAC GGTTGGATCA GGATGAGAGT 
    TTTTCCCAGA GGCTTCCACT TAATATTGAA GAGGCTAAAG ACCGTCTCCG CATTCTGATG 
    CTTCGCAAAC ACCCAAGGTC TCTCTTGATC TTGGATGATG TTTGGGACTC TTGGGTGTTG 
    AAAGCTTTTG ACAGTCAGTG TCAGATTCTT CTTACAACCA GAGACAAGAG TGTTACAGAT 
    TCAGTAATGG GTCCTAAATA TGTAGTCCCT GTGGAGAGTT CCTTAGGAAA GGAAAAAGGA 
    CTTGAAATTT TATCCCTTTT TGTTAATATG AAGAAGGCAG ATTTGCCAGA ACAAGCTCAT 
    AGTATTATAA AAGAATGTAA AGGCTCTCCC CTTGTAGTAT CTTTAATTGG TGCACTTTTA 
    CGTGATTTTC CCAATCGCTG GGAGTACTAC CTCAAACAGC TTCAGAATAA GCAGTTTAAG 
    AGAATAAGGA AATCTTCGTC TTATGATTAT GAGGCTCTAG ATGAAGCCAT GTCTATAAGT 
    GTTGAAATGC TCAGAGAAGA CATCAAAGAT TATTACACAG ATCTTTCCAT CCTTCAGAAG 
    GACGTTAAGG TGCCTACAAA GGTGTTATGT ATTCTCTGGG ACATGGAAAC TGAAGAAGTT 
    GAAGACATAC TGCAGGAGTT TGTAAATAAG TCTCTTTTAT TCTGTGATCG GAATGGAAAG 
    TCGTTTCGTT ATTATTTACA TGATCTTCAA GTAGATTTTC TTACAGAGAA GAATTGCAGC 
    CAGCTTCAGG ATCTACATAA GAAGATAATC ACTCAGTTTC AGAGATATCA CCAGCCGCAT 
    ACTCTTTCAC CAGATCAGGA AGACTGTATG TATTGGTACA ACTTTCTGGC CTATCACATG 
    GCCAGTGCCA AGATGCACAA GGAACTTTGT GCTTTAATGT TTTCCCTGGA TTGGATTAAA 
    GCAAAAACAG AACTTGTAGG CCCTGCTCAT CTGATTCATG AATTTGTGGA ATACAGACAT 
    ATACTAGATG AAAAGGATTG TGCAGTCAGT GAGAATTTTC AGGAGTTTTT ATCTTTAAAT 
    GGACACCTTC TTGGACGACA GCCATTTCCT AATATTGTAC AACTGGGTCT CTGTGAGCCG 
    GAAACTTCAG AAGTTTATCA GCAAGCTAAG CTGCAGGCCA AGCAGGAGGT CGATAATGGA 
    ATGCTTTACC TGGAATGGAT AAACAAAAAA AACATCACGA ATCTTTCCCG CTTAGTTGTC 
    CGCCCCCACA CAGATGCTGT TTACCATGCC TGCTTTTCTG AGGATGGTCA GAGAATAGCT 
    TCTTGTGGAG CTGATAAAAC CTTACAGGTG TTCAAAGCTG AAACAGGAGA GAAACTTCTA 
    GAAATCAAGG CTCATGAGGA TGAAGTGCTT TGTTGTGCAT TCTCTACAGA TGACAGATTT 
    ATAGCAACCT GCTCAGTGGA TAAAAAAGTG AAGATTTGGA ATTCTATGAC TGGGGAACTA 
    GTACACACCT ATGATGAGCA CTCAGAGCAA GTCAATTGCT GCCATTTCAC CAACAGTAGT 
    CATCATCTTC TCTTAGCCAC TGGGTCAAGT GACTGCTTCC TCAAACTTTG GGATTTGAAT 
    CAAAAAGAAT GTCGAAATAC CATGTTTGGT CATACAAATT CAGTCAATCA CTGCAGATTT 
    TCACCAGATG ATAAGCTTTT GGCTAGTTGT TCAGCTGATG GAACCTTAAA GCTTTGGGAT 
    GCGACATCAG CAAATGAGAG GAAAAGCATT AATGTGAAAC AGTTCTTCCT AAATTTGGAG 
    GACCCTCAAG AGGATATGGA AGTGATAGTG AAGTGTTGTT CGTGGTCTGC TGATGGTGCA 
    AGGATAATGG TGGCAGCAAA AAATAAAATC TTTTTGTGGA ATACAGACTC ACGTTCAAAG 
    GTGGCTGATT GCAGAGGACA TTTAAGTTGG GTTCATGGTG TGATGTTTTC TCCTGATGGA 
    TCATCATTTT TGACATCTTC TGATGACCAG ACAATCAGGC TCTGGGAGAC AAAGAAAGTA 
    TGTAAGAACT CTGCTGTAAT GTTAAAGCAA GAAGTAGATG TTGTGTTTCA AGAAAATGAA 
    GTGATGGTCC TTGCAGTTGA CCATATAAGA CGTCTGCAAC TCATTAATGG AAGAACAGGT 
    CAGATTGATT ATCTGACTGA AGCTCAAGTT AGCTGCTGTT GCTTAAGTCC ACATCTTCAG 
    TACATTGCAT TTGGAGATGA AAATGGAGCC ATTGAGATTT TAGAACTTGT AAACAATAGA 
    ATCTTCCAGT CCAGGTTTCA GCACAAGAAA ACTGTATGGC ACATCCAGTT CACAGCCGAT 
    GAGAAGACTC TTATTTCAAG TTCTGATGAT GCTGAAATTC AGGTATGGAA TTGGCAATTG 
    GACAAATGTA TCTTTCTACG AGGCCATCAG GAAACAGTGA AAGACTTTAG ACTCTTGAAA 
    AATTCAAGAC TGCTTTCTTG GTCATTTGAT GGAACAGTGA AGGTATGGAA TATTATTACT 
    GGAAATAAAG AAAAAGACTT TGTCTGTCAC CAGGGTACAG TACTTTCTTG TGACATTTCT 
    CACGATGCTA CCAAGTTTTC ATCTACCTCT GCTGACAAGA CTGCAAAGAT CTGGAGTTTT 
    GATCTCCTTT TGCCACTTCA TGAATTGAGG GGCCACAACG GCTGTGTGCG CTGCTCTGCC 
    TTCTCTGTGG ACAGTACCCT GCTGGCAACG GGAGATGACA ATGGAGAAAT CAGGATATGG 
    AATGTCTCAA ACGGTGAGCT TCTTCATTTG TGTGCTCCGC TTTCAGAAGA AGGAGCTGCT 
    ACCCATGGAG GCTGGGTGAC TGACCTTTGC TTTTCTCCAG ATGGCAAAAT GCTTATCTCT 
    GCTGGAGGAT ATATTAAGTG GTGGAACGTT GTCACTGGGG AATCCTCACA GACCTTCTAC 
    ACAAATGGAA CCAATCTTAA GAAAATACAC GTGTCCCCTG ACTTCAAAAC ATATGTGACT 
    GTGGATAATC TTGGTATTTT ATATATTTTA CAGACTTTAG AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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