APAF1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	APAF1
Entrez Gene ID	58131
Full Name	apoptotic peptidase activating factor 1
Synonyms	apaf-1,zgc:136543
General protein information	Preferred Names apoptotic peptidase activating factor 1 Names apoptotic protease-activating factor 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005164770.4	XP_005164827.1	apoptotic protease-activating factor 1 isoform X1
XM_021475139.1	XP_021330814.1	apoptotic protease-activating factor 1 isoform X1
NM_131608.1	NP_571683.1	apoptotic protease-activating factor 1
XM_005164770.3	XP_005164827.1	apoptotic protease-activating factor 1 isoform X1
XM_005164769.2	XP_005164826.1	apoptotic protease-activating factor 1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	APAF1	NP_863651.1
Canis lupus familiaris (dog)	APAF1	XP_003432082.1
Bos taurus (cattle)	APAF1	NP_001178436.1
Xenopus tropicalis (tropical clawed frog)	apaf1	NP_001106546.1
Gallus gallus (chicken)	APAF1	XP_416167.3
Danio rerio (zebrafish)	apaf1	NP_571683.1
Pan troglodytes (chimpanzee)	APAF1	XP_003313928.1
	APAF1	XP_001086945.1
Mus musculus (house mouse)	Apaf1	NP_033814.2
Rattus norvegicus (Norway rat)	Apaf1	NP_076469.1

Gene Ontology

The following APAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03488	NM_131608.1 Latest version!	Danio rerio apoptotic peptidase activating factor 1 (apaf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa09393	XM_021475139.1 Latest version!	Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa09393	XM_005164769.2 Latest version!	Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa09393	XM_005164770.4 Latest version!	Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa09393	XM_005164770.3	Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

APAF1 ( NM_131608.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_571683.1 Danio rerio apoptotic peptidase activating factor 1 (apaf1), mRNA.

Price & Availability↑

CloneID	ODa03488
Clone ID Related Accession (Same CDS sequence)	NM_131608.1
Accession Version	NM_131608.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Danio rerio(zebrafish)
Product	apoptotic protease-activating factor 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF251502.1. On Jun 7, 2012 this sequence version replaced NM_001045243.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF251502.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA 
GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG 
TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT 
GGAGATCTGG CCAGTCTCCT TCACAGTGAT CTGCCTCTGC TCAGCCCTGA GGGAGAGAAG 
AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT 
CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG 
TACCAGCTGC GGGACACACC GGGCTGGGTG ACTGTATTTG GCATGGCTGG CTCAGGGAAG 
TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT 
GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG 
TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC 
ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT 
CTGCTGATCC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC 
AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT 
GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC 
GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA 
GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG 
AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC 
TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA 
CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG 
GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT 
GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CATATCGATA TTACCTTCAT 
GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA 
AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA 
GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG 
GAGCTTTATT CACTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA 
TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT 
GAAGTGCGAG TGCAGTTTCA GGAGTTCCTT TCTCTGAATG GTCATCATCT GGAGCAGAGG 
CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCTGA GGTTTACAGA 
CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG 
AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC 
TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA 
CTTCGGGTGT TTAAATCGAC CTCTGGAGAG AAGCTTTTGG AGCTTCAGGC TCACGAGGAA 
GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC 
AGGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA 
CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC 
ACCTGCTCAA ATGACAAATT CACTAACACC AGGTTGTGGA ACCCTAACAA AAAGACGTCT 
CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC 
CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG 
AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG 
GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA 
AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGAAGCT GAAGACGAGT 
CGTTTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT 
CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC 
AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG 
TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT 
GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTCCTCTC ACAGTGATGC TACGATCATC 
GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTTGTGCTT 
GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT 
GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA 
TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT 
GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC 
GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT 
TCATCTTCTC CTCATCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG 
ACCAGAGGGC AGATGCTGCA AGACCTTGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT 
GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG 
AGCAGTGCCT CATGGAAAAT GTTATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC 
TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG 
CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTC CTCGGGACAC TAAAGACTCG 
ATGAACTCAT ACCATGCGGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG 
CTCGTCTCTA CTGCGGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA GGCGCTGCAG 
ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC 
TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_571683.1
CDS	1..3786
Translation	MEERARSRLLRSKATLEQDIKASYLMDHMISDGVLTNDEEAKVLSKATRKEQAVALLETLLRKDNRAYISFYNALIRESYGDLASLLHSDLPLLSPEGEKSFADGVSPSVQAILSVGGVPQRPVVFVSRPPLLNLIREMLYQLRDTPGWVTVFGMAGSGKSVMAAEVVRDRSLIKECFPDGVHWLSVGQCERADLLVRMQSLCFRLEQCQSSDTSQRPPSTVEEAKERLRFLMLRRFPRSLLILDDVWDSSSLRSFDIQCRVLLTTRNRALTDSVSGVRYEVPVENGLDEEKALEILALYVNGKMHKLPEQARSIVSECKGSPLVVSLIGALLREFPDRWSYYLRQLQQKQFKRIRKSSSYDYEALDQAMDASLQVLEAEHQELYRDLSVMQKDIKVPAKVLSVLWGLELEEVEDVLQEFVNKSLLFRDCNQRPYRYYLHDLQLDFLAEQNRDQIAELHKKMVRQYQRFYSKRPPDSADKDSLYWYQFIPYHMAKAGLSKELYSLMFSLDWVKEKARIMGSAHLINDYVEYGEILDKENSEVRVQFQEFLSLNGHHLEQRPFPDVVQLALSQPDRSEVYRQALMQAQKRASRGQIYLNWVNKNIEEGLSRLVMHPHQGAVYYACFSKDGSKIASCGASKALRVFKSTSGEKLLELQAHEEDVLCCAFSPDDRHIATCASDRKVKLWNVERGVLIREFEVEHEEQINHCQFTNTGRRVLLATCSNDKFTNTRLWNPNKKTSQNTMFGHMEPVNHCCFSPNDLYLATSSSDGSLKLFEVSSANEWKSIDVDSFFPESDEEIKAMVKCSTWSADGSQIICAARNTVFVFDVETSDLLLKLKTSRLSTIQFCHACPNSSLLAVALSHYTVELWNFESSKKKAECSGHLSWVHCVQFSPDGSLLLSSSDDQTIRLWETDRVHTSSAVALKRDTDVLSSHSDATIIAPDSSNRLQVLSGSTGAVVLESEELSSRIRCSCISRNAAFVALGSEDGTVQVIEVPSSKASVKLSGHTKTVHHCQFTDDCEILITSSEDSTIRVWKWRTGECMVLQGHMEPVRKFHLLSSSSSPHLFSWSFDGTVKVWDLTRGQMLQDLVCHEGAVLSCDVSSDGRLFATTSANRTAKVWSSASWKMLFLLEGHKDCVRSCRFSWDNKRLATGDDNGEIRLWSMLDGALLKICPRDTKDSMNSYHAGWVTDLHFSPDNRVLVSTAGYIKWWSVESGEALQTFYTMGGNLKKIHVSPDFSTFITVDSIGILYILKRLEGEGT

Target ORF information:

RefSeq Version	NM_131608.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio apoptotic peptidase activating factor 1 (apaf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_131608.1

ORF Insert Sequence:

ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA 
GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG 
TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT 
GGAGATCTGG CCAGTCTCCT TCACAGTGAT CTGCCTCTGC TCAGCCCTGA GGGAGAGAAG 
AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT 
CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG 
TACCAGCTGC GGGACACACC GGGCTGGGTG ACTGTATTTG GCATGGCTGG CTCAGGGAAG 
TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT 
GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG 
TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC 
ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT 
CTGCTGATCC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC 
AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT 
GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC 
GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA 
GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG 
AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC 
TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA 
CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG 
GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT 
GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CATATCGATA TTACCTTCAT 
GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA 
AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA 
GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG 
GAGCTTTATT CACTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA 
TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT 
GAAGTGCGAG TGCAGTTTCA GGAGTTCCTT TCTCTGAATG GTCATCATCT GGAGCAGAGG 
CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCTGA GGTTTACAGA 
CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG 
AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC 
TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA 
CTTCGGGTGT TTAAATCGAC CTCTGGAGAG AAGCTTTTGG AGCTTCAGGC TCACGAGGAA 
GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC 
AGGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA 
CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC 
ACCTGCTCAA ATGACAAATT CACTAACACC AGGTTGTGGA ACCCTAACAA AAAGACGTCT 
CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC 
CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG 
AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG 
GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA 
AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGAAGCT GAAGACGAGT 
CGTTTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT 
CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC 
AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG 
TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT 
GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTCCTCTC ACAGTGATGC TACGATCATC 
GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTTGTGCTT 
GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT 
GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA 
TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT 
GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC 
GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT 
TCATCTTCTC CTCATCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG 
ACCAGAGGGC AGATGCTGCA AGACCTTGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT 
GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG 
AGCAGTGCCT CATGGAAAAT GTTATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC 
TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG 
CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTC CTCGGGACAC TAAAGACTCG 
ATGAACTCAT ACCATGCGGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG 
CTCGTCTCTA CTGCGGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA GGCGCTGCAG 
ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC 
TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APAF1 ( XM_005164770.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164827.1 Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa09393
Clone ID Related Accession (Same CDS sequence)	XM_005164770.4 , XM_021475139.1 , XM_005164770.3 , XM_005164769.2
Accession Version	XM_005164770.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	apoptotic protease-activating factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164770.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA 
GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG 
TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT 
GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG 
AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT 
CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG 
TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG 
TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT 
GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG 
TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC 
ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT 
CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC 
AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT 
GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC 
GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA 
GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG 
AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC 
TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA 
CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG 
GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT 
GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT 
GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA 
AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA 
GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG 
GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA 
TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT 
GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG 
CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA 
CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG 
AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC 
TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA 
CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA 
GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC 
AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA 
CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC 
ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT 
CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC 
CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG 
AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG 
GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA 
AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT 
CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT 
CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC 
AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG 
TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT 
GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC 
GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT 
GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT 
GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA 
TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT 
GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC 
GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT 
TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG 
ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT 
GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG 
AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC 
TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG 
CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG 
ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG 
CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG 
ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC 
TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164827.1
CDS	265..4050
Translation	MEERARSRLLRSKATLEQDIKASYLMDHMISDGVLTNDEEAKVLSKATRKEQAVALLETLLRKDNRAYISFYNALIRESYGDLASLLHSDLPLLSPEGEKSFADGVSPSVQAILSVGGVPQRPVVFVSRPPLLNLIREMLYRLRDTPGWVTVFGMAGSGKSVMAAEVVRDRSLIKECFPDGVHWLSVGQCERADLLVRMQSLCFRLEQCQSSDTSQRPPSTVEEAKERLRFLMLRRFPRSLLILDDVWDSSSLRSFDIQCRVLLTTRNRALTDSVSGVRYEVPVENGLDEEKALEILALYVNGKMHKLPEQARSIVSECKGSPLVVSLIGALLREFPDRWSYYLRQLQQKQFKRIRKSSSYDYEALDQAMDASLQVLEAEHQELYRDLSVMQKDIKVPAKVLSVLWGLELEEVEDVLQEFVNKSLLFRDCNQRPYRYYLHDLQLDFLAEQNRDQIAELHKKMVRQYQRFYSKRPPDSADKDSLYWYQFIPYHMAKAGLSKELYSLMFSLDWVKEKARIMGSAHLINDYVEYGEILDKENSEVRVQFQEFLSLNGHHLEQRPFPDVVQLALSQPDRSEVYRQALMQAQKRASRGQIYLNWVNKNIEEGLSRLVMHPHQGAVYYACFSKDGSKIASCGASKALRVFKSTSGEKLLELQAHEEDVLCCAFSPDDRHIATCASDKKVKLWNVERGVLIREFEVEHEEQINHCQFTNTGRRVLLATCSNDKFTNTKLWNPNKKTSQNTMFGHMEPVNHCCFSPNDLYLATSSSDGSLKLFEVSSANEWKSIDVDSFFPESDEEIKAMVKCSTWSADGSQIICAARNTVFVFDVETSDLLLELKTSRLSTIQFCHACPNSSLLAVALSHYTVELWNFESSKKKAECSGHLSWVHCVQFSPDGSLLLSSSDDQTIRLWETDRVHTSSAVALKRDTDVLFSHSDATIIAPDSSNRLQVLSGSTGAVVLESEELSSRIRCSCISRNAAFVALGSEDGTVQVIEVPSSKASVKLSGHTKTVHHCQFTDDCEILITSSEDSTIRVWKWRTGECMVLQGHMEPVRKFHLLSSSSSPRLFSWSFDGTVKVWDLTRGQMLQDLVCHEGAVLSCDVSSDGRLFATTSANRTAKVWSSASWKMIFLLEGHKDCVRSCRFSWDNKRLATGDDNGEIRLWSMLDGALLKICSRDTKDSMDSYHGGWVTDLHFSPDNRVLVSTGGYIKWWSVESGEALQTFYTMGGNLKKIHVSPDFSTFITVDSIGILYILKRLEGEGT

Target ORF information:

RefSeq Version	XM_005164770.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164770.4

ORF Insert Sequence:

ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA 
GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG 
TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT 
GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG 
AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT 
CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG 
TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG 
TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT 
GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG 
TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC 
ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT 
CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC 
AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT 
GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC 
GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA 
GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG 
AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC 
TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA 
CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG 
GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT 
GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT 
GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA 
AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA 
GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG 
GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA 
TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT 
GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG 
CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA 
CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG 
AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC 
TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA 
CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA 
GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC 
AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA 
CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC 
ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT 
CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC 
CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG 
AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG 
GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA 
AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT 
CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT 
CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC 
AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG 
TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT 
GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC 
GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT 
GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT 
GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA 
TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT 
GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC 
GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT 
TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG 
ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT 
GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG 
AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC 
TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG 
CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG 
ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG 
CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG 
ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC 
TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APAF1 ( XM_021475139.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021330814.1 Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X2, mRNA.

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CloneID	ODa09393
Clone ID Related Accession (Same CDS sequence)	XM_005164770.4 , XM_021475139.1 , XM_005164770.3 , XM_005164769.2
Accession Version	XM_021475139.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	apoptotic protease-activating factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA 
GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG 
TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT 
GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG 
AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT 
CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG 
TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG 
TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT 
GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG 
TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC 
ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT 
CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC 
AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT 
GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC 
GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA 
GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG 
AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC 
TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA 
CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG 
GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT 
GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT 
GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA 
AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA 
GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG 
GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA 
TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT 
GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG 
CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA 
CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG 
AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC 
TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA 
CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA 
GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC 
AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA 
CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC 
ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT 
CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC 
CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG 
AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG 
GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA 
AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT 
CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT 
CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC 
AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG 
TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT 
GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC 
GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT 
GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT 
GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA 
TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT 
GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC 
GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT 
TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG 
ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT 
GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG 
AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC 
TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG 
CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG 
ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG 
CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG 
ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC 
TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330814.1
CDS	102..3887
Translation	MEERARSRLLRSKATLEQDIKASYLMDHMISDGVLTNDEEAKVLSKATRKEQAVALLETLLRKDNRAYISFYNALIRESYGDLASLLHSDLPLLSPEGEKSFADGVSPSVQAILSVGGVPQRPVVFVSRPPLLNLIREMLYRLRDTPGWVTVFGMAGSGKSVMAAEVVRDRSLIKECFPDGVHWLSVGQCERADLLVRMQSLCFRLEQCQSSDTSQRPPSTVEEAKERLRFLMLRRFPRSLLILDDVWDSSSLRSFDIQCRVLLTTRNRALTDSVSGVRYEVPVENGLDEEKALEILALYVNGKMHKLPEQARSIVSECKGSPLVVSLIGALLREFPDRWSYYLRQLQQKQFKRIRKSSSYDYEALDQAMDASLQVLEAEHQELYRDLSVMQKDIKVPAKVLSVLWGLELEEVEDVLQEFVNKSLLFRDCNQRPYRYYLHDLQLDFLAEQNRDQIAELHKKMVRQYQRFYSKRPPDSADKDSLYWYQFIPYHMAKAGLSKELYSLMFSLDWVKEKARIMGSAHLINDYVEYGEILDKENSEVRVQFQEFLSLNGHHLEQRPFPDVVQLALSQPDRSEVYRQALMQAQKRASRGQIYLNWVNKNIEEGLSRLVMHPHQGAVYYACFSKDGSKIASCGASKALRVFKSTSGEKLLELQAHEEDVLCCAFSPDDRHIATCASDKKVKLWNVERGVLIREFEVEHEEQINHCQFTNTGRRVLLATCSNDKFTNTKLWNPNKKTSQNTMFGHMEPVNHCCFSPNDLYLATSSSDGSLKLFEVSSANEWKSIDVDSFFPESDEEIKAMVKCSTWSADGSQIICAARNTVFVFDVETSDLLLELKTSRLSTIQFCHACPNSSLLAVALSHYTVELWNFESSKKKAECSGHLSWVHCVQFSPDGSLLLSSSDDQTIRLWETDRVHTSSAVALKRDTDVLFSHSDATIIAPDSSNRLQVLSGSTGAVVLESEELSSRIRCSCISRNAAFVALGSEDGTVQVIEVPSSKASVKLSGHTKTVHHCQFTDDCEILITSSEDSTIRVWKWRTGECMVLQGHMEPVRKFHLLSSSSSPRLFSWSFDGTVKVWDLTRGQMLQDLVCHEGAVLSCDVSSDGRLFATTSANRTAKVWSSASWKMIFLLEGHKDCVRSCRFSWDNKRLATGDDNGEIRLWSMLDGALLKICSRDTKDSMDSYHGGWVTDLHFSPDNRVLVSTGGYIKWWSVESGEALQTFYTMGGNLKKIHVSPDFSTFITVDSIGILYILKRLEGEGT

Target ORF information:

RefSeq Version	XM_021475139.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021475139.1

ORF Insert Sequence:

ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA 
GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG 
TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT 
GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG 
AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT 
CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG 
TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG 
TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT 
GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG 
TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC 
ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT 
CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC 
AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT 
GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC 
GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA 
GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG 
AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC 
TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA 
CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG 
GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT 
GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT 
GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA 
AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA 
GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG 
GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA 
TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT 
GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG 
CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA 
CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG 
AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC 
TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA 
CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA 
GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC 
AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA 
CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC 
ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT 
CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC 
CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG 
AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG 
GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA 
AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT 
CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT 
CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC 
AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG 
TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT 
GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC 
GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT 
GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT 
GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA 
TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT 
GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC 
GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT 
TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG 
ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT 
GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG 
AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC 
TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG 
CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG 
ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG 
CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG 
ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC 
TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APAF1 ( XM_005164770.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164827.1 Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa09393
Clone ID Related Accession (Same CDS sequence)	XM_005164770.4 , XM_021475139.1 , XM_005164770.3 , XM_005164769.2
Accession Version	XM_005164770.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	apoptotic protease-activating factor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164770.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA 
GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG 
TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT 
GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG 
AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT 
CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG 
TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG 
TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT 
GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG 
TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC 
ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT 
CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC 
AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT 
GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC 
GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA 
GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG 
AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC 
TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA 
CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG 
GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT 
GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT 
GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA 
AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA 
GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG 
GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA 
TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT 
GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG 
CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA 
CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG 
AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC 
TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA 
CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA 
GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC 
AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA 
CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC 
ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT 
CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC 
CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG 
AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG 
GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA 
AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT 
CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT 
CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC 
AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG 
TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT 
GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC 
GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT 
GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT 
GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA 
TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT 
GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC 
GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT 
TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG 
ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT 
GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG 
AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC 
TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG 
CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG 
ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG 
CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG 
ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC 
TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164827.1
CDS	248..4033
Translation	MEERARSRLLRSKATLEQDIKASYLMDHMISDGVLTNDEEAKVLSKATRKEQAVALLETLLRKDNRAYISFYNALIRESYGDLASLLHSDLPLLSPEGEKSFADGVSPSVQAILSVGGVPQRPVVFVSRPPLLNLIREMLYRLRDTPGWVTVFGMAGSGKSVMAAEVVRDRSLIKECFPDGVHWLSVGQCERADLLVRMQSLCFRLEQCQSSDTSQRPPSTVEEAKERLRFLMLRRFPRSLLILDDVWDSSSLRSFDIQCRVLLTTRNRALTDSVSGVRYEVPVENGLDEEKALEILALYVNGKMHKLPEQARSIVSECKGSPLVVSLIGALLREFPDRWSYYLRQLQQKQFKRIRKSSSYDYEALDQAMDASLQVLEAEHQELYRDLSVMQKDIKVPAKVLSVLWGLELEEVEDVLQEFVNKSLLFRDCNQRPYRYYLHDLQLDFLAEQNRDQIAELHKKMVRQYQRFYSKRPPDSADKDSLYWYQFIPYHMAKAGLSKELYSLMFSLDWVKEKARIMGSAHLINDYVEYGEILDKENSEVRVQFQEFLSLNGHHLEQRPFPDVVQLALSQPDRSEVYRQALMQAQKRASRGQIYLNWVNKNIEEGLSRLVMHPHQGAVYYACFSKDGSKIASCGASKALRVFKSTSGEKLLELQAHEEDVLCCAFSPDDRHIATCASDKKVKLWNVERGVLIREFEVEHEEQINHCQFTNTGRRVLLATCSNDKFTNTKLWNPNKKTSQNTMFGHMEPVNHCCFSPNDLYLATSSSDGSLKLFEVSSANEWKSIDVDSFFPESDEEIKAMVKCSTWSADGSQIICAARNTVFVFDVETSDLLLELKTSRLSTIQFCHACPNSSLLAVALSHYTVELWNFESSKKKAECSGHLSWVHCVQFSPDGSLLLSSSDDQTIRLWETDRVHTSSAVALKRDTDVLFSHSDATIIAPDSSNRLQVLSGSTGAVVLESEELSSRIRCSCISRNAAFVALGSEDGTVQVIEVPSSKASVKLSGHTKTVHHCQFTDDCEILITSSEDSTIRVWKWRTGECMVLQGHMEPVRKFHLLSSSSSPRLFSWSFDGTVKVWDLTRGQMLQDLVCHEGAVLSCDVSSDGRLFATTSANRTAKVWSSASWKMIFLLEGHKDCVRSCRFSWDNKRLATGDDNGEIRLWSMLDGALLKICSRDTKDSMDSYHGGWVTDLHFSPDNRVLVSTGGYIKWWSVESGEALQTFYTMGGNLKKIHVSPDFSTFITVDSIGILYILKRLEGEGT

Target ORF information:

RefSeq Version	XM_005164770.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164770.3

ORF Insert Sequence:

ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA 
GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG 
TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT 
GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG 
AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT 
CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG 
TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG 
TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT 
GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG 
TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC 
ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT 
CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC 
AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT 
GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC 
GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA 
GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG 
AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC 
TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA 
CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG 
GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT 
GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT 
GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA 
AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA 
GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG 
GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA 
TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT 
GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG 
CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA 
CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG 
AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC 
TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA 
CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA 
GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC 
AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA 
CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC 
ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT 
CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC 
CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG 
AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG 
GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA 
AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT 
CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT 
CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC 
AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG 
TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT 
GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC 
GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT 
GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT 
GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA 
TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT 
GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC 
GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT 
TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG 
ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT 
GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG 
AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC 
TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG 
CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG 
ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG 
CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG 
ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC 
TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

APAF1 ( XM_005164769.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005164826.1 Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa09393
Clone ID Related Accession (Same CDS sequence)	XM_005164770.4 , XM_021475139.1 , XM_005164770.3 , XM_005164769.2
Accession Version	XM_005164769.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	apoptotic protease-activating factor 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA 
GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG 
TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT 
GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG 
AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT 
CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG 
TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG 
TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT 
GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG 
TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC 
ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT 
CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC 
AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT 
GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC 
GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA 
GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG 
AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC 
TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA 
CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG 
GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT 
GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT 
GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA 
AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA 
GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG 
GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA 
TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT 
GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG 
CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA 
CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG 
AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC 
TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA 
CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA 
GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC 
AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA 
CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC 
ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT 
CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC 
CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG 
AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG 
GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA 
AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT 
CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT 
CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC 
AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG 
TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT 
GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC 
GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT 
GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT 
GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA 
TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT 
GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC 
GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT 
TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG 
ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT 
GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG 
AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC 
TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG 
CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG 
ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG 
CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG 
ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC 
TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164826.1
CDS	181..3966
Translation	MEERARSRLLRSKATLEQDIKASYLMDHMISDGVLTNDEEAKVLSKATRKEQAVALLETLLRKDNRAYISFYNALIRESYGDLASLLHSDLPLLSPEGEKSFADGVSPSVQAILSVGGVPQRPVVFVSRPPLLNLIREMLYRLRDTPGWVTVFGMAGSGKSVMAAEVVRDRSLIKECFPDGVHWLSVGQCERADLLVRMQSLCFRLEQCQSSDTSQRPPSTVEEAKERLRFLMLRRFPRSLLILDDVWDSSSLRSFDIQCRVLLTTRNRALTDSVSGVRYEVPVENGLDEEKALEILALYVNGKMHKLPEQARSIVSECKGSPLVVSLIGALLREFPDRWSYYLRQLQQKQFKRIRKSSSYDYEALDQAMDASLQVLEAEHQELYRDLSVMQKDIKVPAKVLSVLWGLELEEVEDVLQEFVNKSLLFRDCNQRPYRYYLHDLQLDFLAEQNRDQIAELHKKMVRQYQRFYSKRPPDSADKDSLYWYQFIPYHMAKAGLSKELYSLMFSLDWVKEKARIMGSAHLINDYVEYGEILDKENSEVRVQFQEFLSLNGHHLEQRPFPDVVQLALSQPDRSEVYRQALMQAQKRASRGQIYLNWVNKNIEEGLSRLVMHPHQGAVYYACFSKDGSKIASCGASKALRVFKSTSGEKLLELQAHEEDVLCCAFSPDDRHIATCASDKKVKLWNVERGVLIREFEVEHEEQINHCQFTNTGRRVLLATCSNDKFTNTKLWNPNKKTSQNTMFGHMEPVNHCCFSPNDLYLATSSSDGSLKLFEVSSANEWKSIDVDSFFPESDEEIKAMVKCSTWSADGSQIICAARNTVFVFDVETSDLLLELKTSRLSTIQFCHACPNSSLLAVALSHYTVELWNFESSKKKAECSGHLSWVHCVQFSPDGSLLLSSSDDQTIRLWETDRVHTSSAVALKRDTDVLFSHSDATIIAPDSSNRLQVLSGSTGAVVLESEELSSRIRCSCISRNAAFVALGSEDGTVQVIEVPSSKASVKLSGHTKTVHHCQFTDDCEILITSSEDSTIRVWKWRTGECMVLQGHMEPVRKFHLLSSSSSPRLFSWSFDGTVKVWDLTRGQMLQDLVCHEGAVLSCDVSSDGRLFATTSANRTAKVWSSASWKMIFLLEGHKDCVRSCRFSWDNKRLATGDDNGEIRLWSMLDGALLKICSRDTKDSMDSYHGGWVTDLHFSPDNRVLVSTGGYIKWWSVESGEALQTFYTMGGNLKKIHVSPDFSTFITVDSIGILYILKRLEGEGT

Target ORF information:

RefSeq Version	XM_005164769.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164769.2

ORF Insert Sequence:

ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA 
GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG 
TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT 
GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG 
AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT 
CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG 
TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG 
TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT 
GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG 
TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC 
ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT 
CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC 
AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT 
GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC 
GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA 
GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG 
AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC 
TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA 
CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG 
GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT 
GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT 
GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA 
AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA 
GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG 
GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA 
TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT 
GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG 
CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA 
CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG 
AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC 
TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA 
CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA 
GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC 
AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA 
CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC 
ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT 
CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC 
CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG 
AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG 
GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA 
AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT 
CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT 
CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC 
AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG 
TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT 
GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC 
GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT 
GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT 
GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA 
TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT 
GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC 
GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT 
TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG 
ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT 
GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG 
AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC 
TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG 
CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG 
ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG 
CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG 
ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC 
TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA 
ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

見積依頼

Eメール

japanmarket@genscript.com

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03-6811-6572

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03-6811-6573
050-3174-7589(受注専用)

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オンライン注文

APAF1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報