APAF1 cDNA ORF clone, Danio rerio(Zebrafish)

The following APAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa03488 NM_131608.1
Latest version!
Danio rerio apoptotic peptidase activating factor 1 (apaf1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa09393 XM_021475139.1
Latest version!
Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa09393 XM_005164769.2
Latest version!
Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa09393 XM_005164770.4
Latest version!
Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa09393 XM_005164770.3 Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa03488
    Clone ID Related Accession (Same CDS sequence) NM_131608.1
    Accession Version NM_131608.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-02
    Organism Danio rerio(zebrafish)
    Product apoptotic protease-activating factor 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF251502.1. On Jun 7, 2012 this sequence version replaced NM_001045243.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF251502.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
    AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA
    GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG
    TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT
    GGAGATCTGG CCAGTCTCCT TCACAGTGAT CTGCCTCTGC TCAGCCCTGA GGGAGAGAAG
    AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT
    CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG
    TACCAGCTGC GGGACACACC GGGCTGGGTG ACTGTATTTG GCATGGCTGG CTCAGGGAAG
    TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT
    GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG
    TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC
    ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT
    CTGCTGATCC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC
    AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT
    GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC
    GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA
    GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG
    AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC
    TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA
    CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG
    GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT
    GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CATATCGATA TTACCTTCAT
    GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA
    AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA
    GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG
    GAGCTTTATT CACTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA
    TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT
    GAAGTGCGAG TGCAGTTTCA GGAGTTCCTT TCTCTGAATG GTCATCATCT GGAGCAGAGG
    CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCTGA GGTTTACAGA
    CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG
    AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC
    TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA
    CTTCGGGTGT TTAAATCGAC CTCTGGAGAG AAGCTTTTGG AGCTTCAGGC TCACGAGGAA
    GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC
    AGGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA
    CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC
    ACCTGCTCAA ATGACAAATT CACTAACACC AGGTTGTGGA ACCCTAACAA AAAGACGTCT
    CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC
    CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG
    AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG
    GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA
    AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGAAGCT GAAGACGAGT
    CGTTTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT
    CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC
    AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG
    TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT
    GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTCCTCTC ACAGTGATGC TACGATCATC
    GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTTGTGCTT
    GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT
    GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA
    TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT
    GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC
    GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT
    TCATCTTCTC CTCATCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG
    ACCAGAGGGC AGATGCTGCA AGACCTTGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT
    GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG
    AGCAGTGCCT CATGGAAAAT GTTATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC
    TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG
    CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTC CTCGGGACAC TAAAGACTCG
    ATGAACTCAT ACCATGCGGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG
    CTCGTCTCTA CTGCGGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA GGCGCTGCAG
    ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC
    TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_571683.1
    CDS1..3786
    Translation

    Target ORF information:

    RefSeq Version NM_131608.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio apoptotic peptidase activating factor 1 (apaf1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_131608.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
    AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA
    GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG
    TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT
    GGAGATCTGG CCAGTCTCCT TCACAGTGAT CTGCCTCTGC TCAGCCCTGA GGGAGAGAAG
    AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT
    CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG
    TACCAGCTGC GGGACACACC GGGCTGGGTG ACTGTATTTG GCATGGCTGG CTCAGGGAAG
    TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT
    GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG
    TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC
    ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT
    CTGCTGATCC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC
    AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT
    GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC
    GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA
    GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG
    AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC
    TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA
    CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG
    GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT
    GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CATATCGATA TTACCTTCAT
    GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA
    AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA
    GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG
    GAGCTTTATT CACTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA
    TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT
    GAAGTGCGAG TGCAGTTTCA GGAGTTCCTT TCTCTGAATG GTCATCATCT GGAGCAGAGG
    CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCTGA GGTTTACAGA
    CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG
    AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC
    TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA
    CTTCGGGTGT TTAAATCGAC CTCTGGAGAG AAGCTTTTGG AGCTTCAGGC TCACGAGGAA
    GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC
    AGGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA
    CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC
    ACCTGCTCAA ATGACAAATT CACTAACACC AGGTTGTGGA ACCCTAACAA AAAGACGTCT
    CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC
    CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG
    AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG
    GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA
    AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGAAGCT GAAGACGAGT
    CGTTTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT
    CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC
    AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG
    TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT
    GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTCCTCTC ACAGTGATGC TACGATCATC
    GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTTGTGCTT
    GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT
    GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA
    TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT
    GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC
    GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT
    TCATCTTCTC CTCATCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG
    ACCAGAGGGC AGATGCTGCA AGACCTTGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT
    GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG
    AGCAGTGCCT CATGGAAAAT GTTATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC
    TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG
    CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTC CTCGGGACAC TAAAGACTCG
    ATGAACTCAT ACCATGCGGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG
    CTCGTCTCTA CTGCGGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA GGCGCTGCAG
    ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC
    TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa09393
    Clone ID Related Accession (Same CDS sequence) XM_005164770.4 , XM_021475139.1 , XM_005164770.3 , XM_005164769.2
    Accession Version XM_005164770.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product apoptotic protease-activating factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164770.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
    AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA
    GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG
    TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT
    GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG
    AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT
    CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG
    TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG
    TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT
    GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG
    TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC
    ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT
    CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC
    AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT
    GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC
    GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA
    GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG
    AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC
    TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA
    CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG
    GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT
    GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT
    GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA
    AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA
    GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG
    GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA
    TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT
    GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG
    CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA
    CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG
    AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC
    TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA
    CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA
    GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC
    AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA
    CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC
    ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT
    CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC
    CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG
    AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG
    GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA
    AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT
    CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT
    CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC
    AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG
    TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT
    GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC
    GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT
    GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT
    GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA
    TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT
    GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC
    GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT
    TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG
    ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT
    GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG
    AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC
    TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG
    CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG
    ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG
    CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG
    ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC
    TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005164827.1
    CDS265..4050
    Translation

    Target ORF information:

    RefSeq Version XM_005164770.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005164770.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
    AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA
    GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG
    TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT
    GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG
    AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT
    CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG
    TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG
    TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT
    GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG
    TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC
    ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT
    CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC
    AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT
    GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC
    GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA
    GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG
    AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC
    TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA
    CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG
    GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT
    GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT
    GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA
    AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA
    GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG
    GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA
    TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT
    GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG
    CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA
    CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG
    AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC
    TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA
    CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA
    GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC
    AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA
    CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC
    ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT
    CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC
    CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG
    AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG
    GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA
    AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT
    CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT
    CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC
    AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG
    TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT
    GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC
    GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT
    GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT
    GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA
    TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT
    GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC
    GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT
    TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG
    ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT
    GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG
    AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC
    TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG
    CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG
    ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG
    CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG
    ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC
    TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa09393
    Clone ID Related Accession (Same CDS sequence) XM_005164770.4 , XM_021475139.1 , XM_005164770.3 , XM_005164769.2
    Accession Version XM_021475139.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product apoptotic protease-activating factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
    AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA
    GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG
    TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT
    GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG
    AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT
    CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG
    TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG
    TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT
    GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG
    TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC
    ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT
    CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC
    AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT
    GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC
    GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA
    GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG
    AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC
    TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA
    CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG
    GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT
    GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT
    GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA
    AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA
    GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG
    GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA
    TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT
    GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG
    CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA
    CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG
    AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC
    TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA
    CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA
    GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC
    AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA
    CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC
    ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT
    CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC
    CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG
    AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG
    GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA
    AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT
    CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT
    CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC
    AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG
    TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT
    GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC
    GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT
    GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT
    GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA
    TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT
    GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC
    GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT
    TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG
    ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT
    GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG
    AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC
    TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG
    CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG
    ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG
    CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG
    ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC
    TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021330814.1
    CDS102..3887
    Translation

    Target ORF information:

    RefSeq Version XM_021475139.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021475139.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
    AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA
    GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG
    TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT
    GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG
    AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT
    CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG
    TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG
    TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT
    GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG
    TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC
    ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT
    CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC
    AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT
    GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC
    GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA
    GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG
    AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC
    TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA
    CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG
    GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT
    GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT
    GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA
    AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA
    GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG
    GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA
    TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT
    GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG
    CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA
    CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG
    AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC
    TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA
    CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA
    GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC
    AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA
    CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC
    ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT
    CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC
    CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG
    AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG
    GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA
    AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT
    CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT
    CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC
    AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG
    TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT
    GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC
    GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT
    GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT
    GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA
    TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT
    GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC
    GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT
    TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG
    ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT
    GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG
    AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC
    TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG
    CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG
    ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG
    CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG
    ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC
    TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa09393
    Clone ID Related Accession (Same CDS sequence) XM_005164770.4 , XM_021475139.1 , XM_005164770.3 , XM_005164769.2
    Accession Version XM_005164770.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product apoptotic protease-activating factor 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164770.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
    AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA
    GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG
    TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT
    GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG
    AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT
    CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG
    TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG
    TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT
    GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG
    TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC
    ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT
    CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC
    AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT
    GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC
    GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA
    GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG
    AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC
    TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA
    CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG
    GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT
    GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT
    GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA
    AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA
    GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG
    GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA
    TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT
    GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG
    CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA
    CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG
    AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC
    TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA
    CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA
    GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC
    AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA
    CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC
    ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT
    CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC
    CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG
    AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG
    GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA
    AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT
    CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT
    CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC
    AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG
    TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT
    GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC
    GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT
    GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT
    GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA
    TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT
    GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC
    GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT
    TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG
    ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT
    GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG
    AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC
    TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG
    CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG
    ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG
    CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG
    ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC
    TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005164827.1
    CDS248..4033
    Translation

    Target ORF information:

    RefSeq Version XM_005164770.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005164770.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
    AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA
    GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG
    TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT
    GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG
    AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT
    CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG
    TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG
    TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT
    GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG
    TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC
    ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT
    CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC
    AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT
    GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC
    GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA
    GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG
    AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC
    TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA
    CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG
    GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT
    GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT
    GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA
    AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA
    GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG
    GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA
    TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT
    GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG
    CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA
    CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG
    AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC
    TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA
    CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA
    GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC
    AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA
    CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC
    ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT
    CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC
    CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG
    AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG
    GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA
    AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT
    CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT
    CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC
    AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG
    TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT
    GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC
    GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT
    GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT
    GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA
    TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT
    GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC
    GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT
    TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG
    ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT
    GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG
    AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC
    TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG
    CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG
    ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG
    CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG
    ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC
    TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa09393
    Clone ID Related Accession (Same CDS sequence) XM_005164770.4 , XM_021475139.1 , XM_005164770.3 , XM_005164769.2
    Accession Version XM_005164769.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3786bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product apoptotic protease-activating factor 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
    AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA
    GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG
    TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT
    GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG
    AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT
    CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG
    TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG
    TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT
    GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG
    TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC
    ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT
    CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC
    AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT
    GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC
    GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA
    GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG
    AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC
    TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA
    CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG
    GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT
    GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT
    GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA
    AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA
    GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG
    GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA
    TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT
    GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG
    CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA
    CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG
    AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC
    TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA
    CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA
    GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC
    AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA
    CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC
    ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT
    CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC
    CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG
    AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG
    GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA
    AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT
    CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT
    CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC
    AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG
    TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT
    GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC
    GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT
    GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT
    GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA
    TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT
    GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC
    GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT
    TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG
    ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT
    GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG
    AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC
    TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG
    CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG
    ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG
    CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG
    ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC
    TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005164826.1
    CDS181..3966
    Translation

    Target ORF information:

    RefSeq Version XM_005164769.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio apoptotic peptidase activating factor 1 (apaf1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005164769.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGAAC GTGCCCGCAG CCGCCTGCTG CGCTCCAAAG CCACTCTGGA GCAGGACATC 
    AAAGCCTCCT ACCTGATGGA CCACATGATC AGCGATGGCG TTCTAACCAA TGATGAGGAA
    GCAAAAGTGC TCAGCAAGGC CACTAGAAAA GAGCAGGCCG TGGCTCTGCT TGAGACCCTG
    TTGAGGAAAG ACAATCGTGC CTACATTTCC TTCTACAACG CCCTGATCAG GGAGAGTTAT
    GGAGATCTGG CCAGTCTCCT TCACAGTGAT TTGCCTCTGC TCAGCCCTGA GGGAGAGAAG
    AGCTTCGCTG ATGGAGTCTC TCCCTCTGTC CAGGCGATTC TGAGTGTAGG AGGTGTTCCT
    CAGAGACCTG TGGTGTTCGT GAGTCGACCT CCACTGCTCA ATCTGATCCG GGAGATGCTG
    TACCGGCTGC GGGACACACC GGGCTGGGTA ACTGTATTTG GCATGGCTGG CTCAGGGAAG
    TCTGTGATGG CTGCCGAGGT TGTTCGAGAT CGTTCACTCA TTAAGGAGTG TTTTCCTGAT
    GGTGTTCACT GGCTGTCTGT GGGTCAGTGT GAGCGGGCAG ACCTGTTGGT TAGGATGCAG
    TCTCTGTGCT TTCGTTTGGA GCAGTGTCAA AGTTCAGACA CCAGTCAGCG GCCACCCAGC
    ACTGTGGAGG AGGCCAAAGA GCGCCTGCGC TTCCTAATGC TCCGCAGATT CCCCAGGTCT
    CTGCTGATTC TGGATGATGT CTGGGACAGT TCTTCCCTCA GGTCTTTTGA TATCCAATGC
    AGAGTTCTTC TGACCACACG CAATCGAGCT CTGACTGATT CCGTCAGCGG TGTAAGGTAT
    GAAGTGCCTG TAGAGAACGG TCTGGATGAG GAAAAAGCCT TGGAAATTCT TGCTTTGTAC
    GTTAATGGGA AGATGCACAA ACTCCCAGAA CAGGCCCGCA GCATTGTAAG CGAATGTAAA
    GGTTCTCCTC TGGTGGTTTC TCTGATTGGA GCTCTGCTGA GGGAGTTCCC TGACCGCTGG
    AGCTATTACC TGCGACAGTT GCAGCAGAAG CAGTTCAAGC GCATTCGCAA GTCTTCATCC
    TATGACTATG AAGCACTGGA CCAGGCCATG GACGCTAGCC TGCAGGTTCT GGAGGCCGAA
    CACCAAGAGC TGTACAGAGA CCTGAGCGTC ATGCAGAAAG ACATCAAAGT GCCTGCTAAG
    GTTCTGTCTG TTCTGTGGGG TCTTGAGCTG GAGGAGGTAG AGGACGTCCT GCAGGAGTTT
    GTCAATAAAT CTCTGCTTTT TCGGGACTGT AATCAGCGAC CGTATCGATA TTACCTTCAT
    GATCTGCAGC TGGATTTCCT CGCTGAACAA AACCGTGATC AGATTGCTGA ACTACATAAA
    AAGATGGTTC GTCAGTATCA GAGGTTCTAC AGTAAACGCC CACCTGACTC TGCAGATAAA
    GACTCGTTGT ACTGGTACCA GTTCATCCCG TATCACATGG CTAAAGCAGG ACTGTCGAAG
    GAGCTTTATT CATTGATGTT TTCCTTGGAC TGGGTCAAAG AGAAGGCTCG GATTATGGGA
    TCTGCCCATC TCATCAATGA CTATGTGGAA TATGGGGAAA TACTAGATAA AGAGAATAGT
    GAAGTGCGAG TGCAGTTTCA GGAGTTCCTC TCTCTGAATG GTCATCATCT GGAGCAGAGG
    CCGTTCCCTG ATGTGGTCCA GTTGGCTCTG TCTCAGCCCG ATCGCTCAGA GGTTTACAGA
    CAGGCACTGA TGCAGGCGCA GAAACGGGCC AGCAGAGGGC AGATCTACCT CAACTGGGTG
    AATAAAAATA TTGAGGAGGG TTTGTCTCGG CTAGTCATGC ACCCTCACCA GGGGGCTGTC
    TACTATGCCT GCTTCTCAAA AGACGGGAGT AAAATTGCCT CTTGTGGAGC CAGCAAGGCA
    CTTCGGGTGT TTAAATCAAC CTCTGGAGAG AAGCTTCTGG AGCTTCAGGC TCATGAGGAA
    GATGTTTTAT GCTGTGCCTT TTCCCCTGAT GACCGGCATA TAGCAACATG TGCCAGTGAC
    AAGAAGGTGA AGTTGTGGAA TGTTGAGCGA GGTGTTCTTA TCAGAGAGTT TGAAGTTGAA
    CACGAGGAGC AGATCAACCA CTGCCAGTTC ACCAACACAG GCCGAAGAGT CCTGCTGGCC
    ACCTGCTCAA ATGACAAATT CACTAACACC AAGTTGTGGA ACCCTAACAA AAAGACGTCT
    CAGAACACTA TGTTTGGACA CATGGAGCCG GTCAATCACT GCTGTTTTTC TCCTAATGAC
    CTTTACCTCG CCACTTCATC CAGTGATGGC AGCTTGAAGC TGTTTGAAGT GTCATCTGCG
    AACGAGTGGA AATCAATTGA TGTCGATAGT TTTTTTCCTG AGAGTGACGA AGAAATAAAG
    GCCATGGTGA AATGCAGCAC TTGGTCGGCT GACGGCTCAC AAATCATCTG TGCTGCCAGA
    AACACAGTGT TTGTGTTTGA TGTGGAGACG TCAGACCTGC TGCTGGAGCT GAAGACGAGT
    CGTCTGAGCA CTATTCAGTT CTGTCATGCG TGTCCTAACA GCAGCCTGCT GGCCGTCGCT
    CTCTCGCATT ACACCGTAGA GCTGTGGAAC TTCGAAAGCA GTAAGAAGAA AGCCGAATGC
    AGTGGTCATC TGAGCTGGGT TCACTGTGTG CAGTTTTCAC CCGACGGCTC ACTCCTGCTG
    TCCTCCTCTG ATGATCAGAC TATCAGGTTG TGGGAGACGG ACCGGGTTCA CACTTCTTCT
    GCTGTGGCTC TGAAGAGGGA CACTGATGTG CTGTTCTCTC ACAGTGATGC TACGATCATC
    GCACCCGATA GCAGTAACAG ATTACAGGTG CTGAGTGGAT CTACAGGTGC TGTCGTGCTT
    GAGAGCGAGG AGCTGTCATC CAGGATCCGC TGTTCGTGCA TCAGCAGAAA CGCTGCATTT
    GTGGCTCTGG GATCTGAGGA CGGAACAGTA CAGGTGATTG AGGTTCCTTC TTCAAAGGCA
    TCAGTGAAGC TTTCAGGCCA CACTAAGACT GTTCACCACT GCCAGTTCAC TGACGACTGT
    GAAATCCTTA TAACATCCTC AGAAGATTCC ACTATCCGGG TGTGGAAGTG GAGGACCGGC
    GAGTGTATGG TGCTGCAGGG TCATATGGAG CCGGTCAGGA AGTTTCACCT CTTGAGCTCT
    TCATCTTCTC CTCGTCTGTT CTCATGGTCG TTTGATGGCA CAGTTAAGGT CTGGGATTTG
    ACCAGAGGGC AGATGCTGCA AGACCTCGTG TGTCATGAGG GTGCCGTCCT GTCATGTGAT
    GTGTCCTCTG ATGGACGCCT CTTTGCCACC ACCTCAGCTA ATAGGACTGC TAAGGTGTGG
    AGCAGTGCCT CATGGAAAAT GATATTTTTG TTGGAGGGAC ACAAGGATTG CGTTCGTAGC
    TGCCGTTTTT CTTGGGACAA CAAGAGACTT GCAACTGGCG ATGACAATGG AGAAATCCGG
    CTGTGGAGCA TGCTGGATGG AGCTCTCCTG AAGATCTGTT CTCGGGACAC TAAAGACTCG
    ATGGACTCCT ACCATGGCGG ATGGGTGACT GATCTGCACT TCTCTCCTGA TAACAGGGTG
    CTCGTCTCTA CTGGCGGATA CATCAAGTGG TGGAGCGTGG AGAGCGGAGA AGCCCTGCAG
    ACCTTCTACA CGATGGGAGG CAATCTGAAG AAGATCCACG TGTCTCCTGA CTTCAGCACC
    TTCATCACTG TGGACAGCAT CGGCATCCTT TACATTTTAA AGAGACTTGA GGGAGAGGGA
    ACATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.