APAF1 cDNA ORF clone, Gallus gallus(chicken)

The following APAF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the APAF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa01653 XM_004937654.3
Latest version!
Gallus gallus apoptotic peptidase activating factor 1 (APAF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OGa29640 XM_015289686.1
Latest version!
Gallus gallus apoptotic peptidase activating factor 1 (APAF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OGa29641 XM_015289693.2
Latest version!
Gallus gallus apoptotic peptidase activating factor 1 (APAF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OGa01652 XM_416167.6
Latest version!
Gallus gallus apoptotic peptidase activating factor 1 (APAF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
OGa01652 XM_416167.5 Gallus gallus apoptotic peptidase activating factor 1 (APAF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OGa29641 XM_015289693.1 Gallus gallus apoptotic peptidase activating factor 1 (APAF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
OGa01653 XM_004937654.2 Gallus gallus apoptotic peptidase activating factor 1 (APAF1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa01653
    Clone ID Related Accession (Same CDS sequence) XM_004937654.2 , XM_004937654.3
    Accession Version XM_004937654.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product apoptotic protease-activating factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_004937654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGATGAGA ACAGTAGAAT GTGTTTAATT ACGAATCGTC AGGCACTGGA GAAAGACATC 
    AAAACCTCTT ACATTATGGA TCATATGGTT GCTGACCAAG TCCTGACATT GCAGGAGGAG
    GAGAAAGTGA AACAACAGAA TACACAGAAG GAGCGAGCAG CTATGCTAAT TAATACTCTT
    CTTACCAAAG ACAATAATGC ATATAGATCT TTTTATAATG CACTGCTTCA TGAAGGGTAC
    CGAGATCTTG CTGCACTTCT TCAGGATGGC ATCCCTGCCT TCTCTTCCAA GAATGGAAAG
    AACGCTATGG ATGAGAGGAC TTCATATGTT AAGACAGCTC TCTGCGAAGG AGGTGTACCA
    CAGAGACCAG TTGTGTTTGT CTCTCGGCCA AAGCTGGTAG ATGATATTAA ACAGAAACTG
    CGCAGCTTAG GAAGTGATCC AGGCTGGGTG ACCGTTTATG GAATGGCAGG TTGTGGAAAG
    ACCGTTTTAA CAGCAGAAGC TTTAAGGGAT CACCAGCTCT TGCGAGGTTG CTTTCCAGGA
    GGAGTTCACT GGATCTCTGT GGGAAAACAG GACAAAGCAG GGCTCCTCAT AAAGCTACAG
    AATCTCTGTA GTAGATTAGA GCATGACTCT ACTCTTCCAC AGAGACCACC ACTTAACATT
    GAGGAGGCCA AAGATCGTCT TCGTTTGCTG ATGCTGCGCA AATACCAGAG GTCTCTTTTG
    GTCCTGGATG ACATTTGGGA TTCCTGGGTA CTAAAAGCAT TTGATAATCA ATGTCAGGTT
    CTTATAACCA GCAGGGATAG GAGTGTGACA GATGCTGTGT CTGGCAATAA ATATGAGATT
    CACGTGGAAA GTGGACTAAC ACATGAGAAA GGACTGGAAG TTTTAGCCCT GTTTGTGAAT
    ATGAAAATAT CAGAACTGCC AGAACAAGCC AGCTGCATTG TAACAGAATG CAAAGGTTCT
    CCTCTTGTGA TATCCTTGAT AGGTGCATTA CTACGAGATT TCCCTAGTCG CTGGGAATAT
    TATCTCAAAC AGCTGCAGAA TAAGCAATTT AAAAGAATAA GAAAGTCTTC ATCTTATGAC
    TATGAAGCCC TTGATGAAGC AATGTCCATA AGTGTAGAGC AACTGAGTAA TGATTTAAAA
    GAATATTATA AAGACCTCTC CATCCTCCCA AAAGATGTTA AAGTGCCTAC TAAGGTGCTC
    TGTATTCTTT GGGATATGGA AACTGAAGAA GTTGAAGATA TACTGCAGGA ATTTGTTAAC
    AAATCACTAT TGTTCTGCGA TCGTAATGGG AATTCATTTC ATTATTATTT ACATGATCTT
    CAACTCGACT TTCTTACAGA GAAGAATTGC AACCAGCTTC AGGAACTACA TAAAAATATA
    GTAAATCAGT ACAAGAGATA TTACAAGCTT CATGTGCCTG TGCTATCCCA AGAGGACTGT
    ATGTACTGGT ATAACTTTCT AGCATATCAC ATGGCTGGTG CCAACATGCA GAAGGAACTC
    CGTGATCTTA TGTTTTCTCT AGATTGGATT AAAGCTAAAA CAGAATTGGT AGGACCTGCT
    CATCTAATTC ATGAATATGT AGAGTATAGT TCAGTCCTGG ATGAAAAGGT ATGTAAAAGG
    ATAGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004937711.1
    CDS123..1751
    Translation

    Target ORF information:

    RefSeq Version XM_004937654.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus apoptotic peptidase activating factor 1 (APAF1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004937654.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGATGAGA ACAGTAGAAT GTGTTTAATT ACGAATCGTC AGGCACTGGA GAAAGACATC 
    AAAACCTCTT ACATTATGGA TCATATGGTT GCTGACCAAG TCCTGACATT GCAGGAGGAG
    GAGAAAGTGA AACAACAGAA TACACAGAAG GAGCGAGCAG CTATGCTAAT TAATACTCTT
    CTTACCAAAG ACAATAATGC ATATAGATCT TTTTATAATG CACTGCTTCA TGAAGGGTAC
    CGAGATCTTG CTGCACTTCT TCAGGATGGC ATCCCTGCCT TCTCTTCCAA GAATGGAAAG
    AACGCTATGG ATGAGAGGAC TTCATATGTT AAGACAGCTC TCTGCGAAGG AGGTGTACCA
    CAGAGACCAG TTGTGTTTGT CTCTCGGCCA AAGCTGGTAG ATGATATTAA ACAGAAACTG
    CGCAGCTTAG GAAGTGATCC AGGCTGGGTG ACCGTTTATG GAATGGCAGG TTGTGGAAAG
    ACCGTTTTAA CAGCAGAAGC TTTAAGGGAT CACCAGCTCT TGCGAGGTTG CTTTCCAGGA
    GGAGTTCACT GGATCTCTGT GGGAAAACAG GACAAAGCAG GGCTCCTCAT AAAGCTACAG
    AATCTCTGTA GTAGATTAGA GCATGACTCT ACTCTTCCAC AGAGACCACC ACTTAACATT
    GAGGAGGCCA AAGATCGTCT TCGTTTGCTG ATGCTGCGCA AATACCAGAG GTCTCTTTTG
    GTCCTGGATG ACATTTGGGA TTCCTGGGTA CTAAAAGCAT TTGATAATCA ATGTCAGGTT
    CTTATAACCA GCAGGGATAG GAGTGTGACA GATGCTGTGT CTGGCAATAA ATATGAGATT
    CACGTGGAAA GTGGACTAAC ACATGAGAAA GGACTGGAAG TTTTAGCCCT GTTTGTGAAT
    ATGAAAATAT CAGAACTGCC AGAACAAGCC AGCTGCATTG TAACAGAATG CAAAGGTTCT
    CCTCTTGTGA TATCCTTGAT AGGTGCATTA CTACGAGATT TCCCTAGTCG CTGGGAATAT
    TATCTCAAAC AGCTGCAGAA TAAGCAATTT AAAAGAATAA GAAAGTCTTC ATCTTATGAC
    TATGAAGCCC TTGATGAAGC AATGTCCATA AGTGTAGAGC AACTGAGTAA TGATTTAAAA
    GAATATTATA AAGACCTCTC CATCCTCCCA AAAGATGTTA AAGTGCCTAC TAAGGTGCTC
    TGTATTCTTT GGGATATGGA AACTGAAGAA GTTGAAGATA TACTGCAGGA ATTTGTTAAC
    AAATCACTAT TGTTCTGCGA TCGTAATGGG AATTCATTTC ATTATTATTT ACATGATCTT
    CAACTCGACT TTCTTACAGA GAAGAATTGC AACCAGCTTC AGGAACTACA TAAAAATATA
    GTAAATCAGT ACAAGAGATA TTACAAGCTT CATGTGCCTG TGCTATCCCA AGAGGACTGT
    ATGTACTGGT ATAACTTTCT AGCATATCAC ATGGCTGGTG CCAACATGCA GAAGGAACTC
    CGTGATCTTA TGTTTTCTCT AGATTGGATT AAAGCTAAAA CAGAATTGGT AGGACCTGCT
    CATCTAATTC ATGAATATGT AGAGTATAGT TCAGTCCTGG ATGAAAAGGT ATGTAAAAGG
    ATAGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa01653
    Clone ID Related Accession (Same CDS sequence) XM_004937654.2 , XM_004937654.3
    Accession Version XM_004937654.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1629bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product apoptotic protease-activating factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_004937654.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGATGAGA ACAGTAGAAT GTGTTTAATT ACGAATCGTC AGGCACTGGA GAAAGACATC 
    AAAACCTCTT ACATTATGGA TCATATGGTT GCTGACCAAG TCCTGACATT GCAGGAGGAG
    GAGAAAGTGA AACAACAGAA TACACAGAAG GAGCGAGCAG CTATGCTAAT TAATACTCTT
    CTTACCAAAG ACAATAATGC ATATAGATCT TTTTATAATG CACTGCTTCA TGAAGGGTAC
    CGAGATCTTG CTGCACTTCT TCAGGATGGC ATCCCTGCCT TCTCTTCCAA GAATGGAAAG
    AACGCTATGG ATGAGAGGAC TTCATATGTT AAGACAGCTC TCTGCGAAGG AGGTGTACCA
    CAGAGACCAG TTGTGTTTGT CTCTCGGCCA AAGCTGGTAG ATGATATTAA ACAGAAACTG
    CGCAGCTTAG GAAGTGATCC AGGCTGGGTG ACCGTTTATG GAATGGCAGG TTGTGGAAAG
    ACCGTTTTAA CAGCAGAAGC TTTAAGGGAT CACCAGCTCT TGCGAGGTTG CTTTCCAGGA
    GGAGTTCACT GGATCTCTGT GGGAAAACAG GACAAAGCAG GGCTCCTCAT AAAGCTACAG
    AATCTCTGTA GTAGATTAGA GCATGACTCT ACTCTTCCAC AGAGACCACC ACTTAACATT
    GAGGAGGCCA AAGATCGTCT TCGTTTGCTG ATGCTGCGCA AATACCAGAG GTCTCTTTTG
    GTCCTGGATG ACATTTGGGA TTCCTGGGTA CTAAAAGCAT TTGATAATCA ATGTCAGGTT
    CTTATAACCA GCAGGGATAG GAGTGTGACA GATGCTGTGT CTGGCAATAA ATATGAGATT
    CACGTGGAAA GTGGACTAAC ACATGAGAAA GGACTGGAAG TTTTAGCCCT GTTTGTGAAT
    ATGAAAATAT CAGAACTGCC AGAACAAGCC AGCTGCATTG TAACAGAATG CAAAGGTTCT
    CCTCTTGTGA TATCCTTGAT AGGTGCATTA CTACGAGATT TCCCTAGTCG CTGGGAATAT
    TATCTCAAAC AGCTGCAGAA TAAGCAATTT AAAAGAATAA GAAAGTCTTC ATCTTATGAC
    TATGAAGCCC TTGATGAAGC AATGTCCATA AGTGTAGAGC AACTGAGTAA TGATTTAAAA
    GAATATTATA AAGACCTCTC CATCCTCCCA AAAGATGTTA AAGTGCCTAC TAAGGTGCTC
    TGTATTCTTT GGGATATGGA AACTGAAGAA GTTGAAGATA TACTGCAGGA ATTTGTTAAC
    AAATCACTAT TGTTCTGCGA TCGTAATGGG AATTCATTTC ATTATTATTT ACATGATCTT
    CAACTCGACT TTCTTACAGA GAAGAATTGC AACCAGCTTC AGGAACTACA TAAAAATATA
    GTAAATCAGT ACAAGAGATA TTACAAGCTT CATGTGCCTG TGCTATCCCA AGAGGACTGT
    ATGTACTGGT ATAACTTTCT AGCATATCAC ATGGCTGGTG CCAACATGCA GAAGGAACTC
    CGTGATCTTA TGTTTTCTCT AGATTGGATT AAAGCTAAAA CAGAATTGGT AGGACCTGCT
    CATCTAATTC ATGAATATGT AGAGTATAGT TCAGTCCTGG ATGAAAAGGT ATGTAAAAGG
    ATAGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004937711.1
    CDS145..1773
    Translation

    Target ORF information:

    RefSeq Version XM_004937654.3
    Organism Gallus gallus(chicken)
    Definition Gallus gallus apoptotic peptidase activating factor 1 (APAF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004937654.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGGATGAGA ACAGTAGAAT GTGTTTAATT ACGAATCGTC AGGCACTGGA GAAAGACATC 
    AAAACCTCTT ACATTATGGA TCATATGGTT GCTGACCAAG TCCTGACATT GCAGGAGGAG
    GAGAAAGTGA AACAACAGAA TACACAGAAG GAGCGAGCAG CTATGCTAAT TAATACTCTT
    CTTACCAAAG ACAATAATGC ATATAGATCT TTTTATAATG CACTGCTTCA TGAAGGGTAC
    CGAGATCTTG CTGCACTTCT TCAGGATGGC ATCCCTGCCT TCTCTTCCAA GAATGGAAAG
    AACGCTATGG ATGAGAGGAC TTCATATGTT AAGACAGCTC TCTGCGAAGG AGGTGTACCA
    CAGAGACCAG TTGTGTTTGT CTCTCGGCCA AAGCTGGTAG ATGATATTAA ACAGAAACTG
    CGCAGCTTAG GAAGTGATCC AGGCTGGGTG ACCGTTTATG GAATGGCAGG TTGTGGAAAG
    ACCGTTTTAA CAGCAGAAGC TTTAAGGGAT CACCAGCTCT TGCGAGGTTG CTTTCCAGGA
    GGAGTTCACT GGATCTCTGT GGGAAAACAG GACAAAGCAG GGCTCCTCAT AAAGCTACAG
    AATCTCTGTA GTAGATTAGA GCATGACTCT ACTCTTCCAC AGAGACCACC ACTTAACATT
    GAGGAGGCCA AAGATCGTCT TCGTTTGCTG ATGCTGCGCA AATACCAGAG GTCTCTTTTG
    GTCCTGGATG ACATTTGGGA TTCCTGGGTA CTAAAAGCAT TTGATAATCA ATGTCAGGTT
    CTTATAACCA GCAGGGATAG GAGTGTGACA GATGCTGTGT CTGGCAATAA ATATGAGATT
    CACGTGGAAA GTGGACTAAC ACATGAGAAA GGACTGGAAG TTTTAGCCCT GTTTGTGAAT
    ATGAAAATAT CAGAACTGCC AGAACAAGCC AGCTGCATTG TAACAGAATG CAAAGGTTCT
    CCTCTTGTGA TATCCTTGAT AGGTGCATTA CTACGAGATT TCCCTAGTCG CTGGGAATAT
    TATCTCAAAC AGCTGCAGAA TAAGCAATTT AAAAGAATAA GAAAGTCTTC ATCTTATGAC
    TATGAAGCCC TTGATGAAGC AATGTCCATA AGTGTAGAGC AACTGAGTAA TGATTTAAAA
    GAATATTATA AAGACCTCTC CATCCTCCCA AAAGATGTTA AAGTGCCTAC TAAGGTGCTC
    TGTATTCTTT GGGATATGGA AACTGAAGAA GTTGAAGATA TACTGCAGGA ATTTGTTAAC
    AAATCACTAT TGTTCTGCGA TCGTAATGGG AATTCATTTC ATTATTATTT ACATGATCTT
    CAACTCGACT TTCTTACAGA GAAGAATTGC AACCAGCTTC AGGAACTACA TAAAAATATA
    GTAAATCAGT ACAAGAGATA TTACAAGCTT CATGTGCCTG TGCTATCCCA AGAGGACTGT
    ATGTACTGGT ATAACTTTCT AGCATATCAC ATGGCTGGTG CCAACATGCA GAAGGAACTC
    CGTGATCTTA TGTTTTCTCT AGATTGGATT AAAGCTAAAA CAGAATTGGT AGGACCTGCT
    CATCTAATTC ATGAATATGT AGAGTATAGT TCAGTCCTGG ATGAAAAGGT ATGTAAAAGG
    ATAGCATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa29640
    Clone ID Related Accession (Same CDS sequence) XM_015289686.1
    Accession Version XM_015289686.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1983bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product apoptotic protease-activating factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGATGAGA ACAGTAGAAT GTGTTTAATT ACGAATCGTC AGGCACTGGA GAAAGACATC 
    AAAACCTCTT ACATTATGGA TCATATGGTT GCTGACCAAG TCCTGACATT GCAGGAGGAG
    GAGAAAGTGA AACAACAGAA TACACAGAAG GAGCGAGCAG CTATGCTAAT TAATACTCTT
    CTTACCAAAG ACAATAATGC ATATAGATCT TTTTATAATG CACTGCTTCA TGAAGGGTAC
    CGAGATCTTG CTGCACTTCT TCAGGATGGC ATCCCTGCCT TCTCTTCCAA GAATGGAAAG
    AACGCTATGG ATGAGAGGAC TTCATATGTT AAGACAGCTC TCTGCGAAGG AGGTGTACCA
    CAGAGACCAG TTGTGTTTGT CTCTCGGCCA AAGCTGGTAG ATGATATTAA ACAGAAACTG
    CGCAGCTTAG GAAGTGATCC AGGCTGGGTG ACCGTTTATG GAATGGCAGG TTGTGGAAAG
    ACCGTTTTAA CAGCAGAAGC TTTAAGGGAT CACCAGCTCT TGCGAGGTTG CTTTCCAGGA
    GGAGTTCACT GGATCTCTGT GGGAAAACAG GACAAAGCAG GGCTCCTCAT AAAGCTACAG
    AATCTCTGTA GTAGATTAGA GCATGACTCT ACTCTTCCAC AGAGACCACC ACTTAACATT
    GAGGAGGCCA AAGATCGTCT TCGTTTGCTG ATGCTGCGCA AATACCAGAG GTCTCTTTTG
    GTCCTGGATG ACATTTGGGA TTCCTGGGTA CTAAAAGCAT TTGATAATCA ATGTCAGGTT
    CTTATAACCA GCAGGGATAG GAGTGTGACA GATGCTGTGT CTGGCAATAA ATATGAGATT
    CACGTGGAAA GTGGACTAAC ACATGAGAAA GGACTGGAAG TTTTAGCCCT GTTTGTGAAT
    ATGAAAATAT CAGAACTGCC AGAACAAGCC AGCTGCATTG TAACAGAATG CAAAGGTTCT
    CCTCTTGTGA TATCCTTGAT AGGTGCATTA CTACGAGATT TCCCTAGTCG CTGGGAATAT
    TATCTCAAAC AGCTGCAGAA TAAGCAATTT AAAAGAATAA GAAAGTCTTC ATCTTATGAC
    TATGAAGCCC TTGATGAAGC AATGTCCATA AGTGTAGAGC AACTGAGTAA TGATTTAAAA
    GAATATTATA AAGACCTCTC CATCCTCCCA AAAGATGTTA AAGTGCCTAC TAAGGTGCTC
    TGTATTCTTT GGGATATGGA AACTGAAGAA GTTGAAGATA TACTGCAGGA ATTTGTTAAC
    AAATCACTAT TGTTCTGCGA TCGTAATGGG AATTCATTTC ATTATTATTT ACATGATCTT
    CAACTCGACT TTCTTACAGA GAAGAATTGC AACCAGCTTC AGGAACTACA TAAAAATATA
    GTAAATCAGT ACAAGAGATA TTACAAGCTT CATGTGCCTG TGCTATCCCA AGAGGACTGT
    ATGTACTGGT ATAACTTTCT AGCATATCAC ATGGCTGGTG CCAACATGCA GAAGGAACTC
    CGTGATCTTA TGTTTTCTCT AGATTGGATT AAAGCTAAAA CAGAATTGGT AGGACCTGCT
    CATCTAATTC ATGAATATGT AGAGTATAGT TCAGTCCTGG ATGAAAAGGA TAGCATAATA
    CGTGAGAATT TTCAGGAATT TCTGTCTTTA AATGGGCACC TTCTTGGACG ACAGCCTTTT
    CCTGACATTA CCCAGCTTGG TCTTTGCCAA CCAGAGACAT CAGAGGTGTA TCAGCAAGCC
    AAGCTACGGG CTCAAAGAGA AACGGGAACA TTTTACTTGG AATGGATAAA TAAAAAATCC
    TTGAAAAACC TTTCCCGTCT GGTTGTTCGT CCGCACAGAG ATGCAGTTTA CCATGCCTGC
    TTTTCTAGGG ATAGACAAAG AATAGCATCA TGTGGAGCTG ATAAGACACT TCAGCAGTCT
    GCCTGGAGGG AAGCCAGACA GCTCTCAGGA AAACTAGCAA TCAGCCAGCC ACTAACCTGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145172.1
    CDS123..2105
    Translation

    Target ORF information:

    RefSeq Version XM_015289686.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus apoptotic peptidase activating factor 1 (APAF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289686.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGATGAGA ACAGTAGAAT GTGTTTAATT ACGAATCGTC AGGCACTGGA GAAAGACATC 
    AAAACCTCTT ACATTATGGA TCATATGGTT GCTGACCAAG TCCTGACATT GCAGGAGGAG
    GAGAAAGTGA AACAACAGAA TACACAGAAG GAGCGAGCAG CTATGCTAAT TAATACTCTT
    CTTACCAAAG ACAATAATGC ATATAGATCT TTTTATAATG CACTGCTTCA TGAAGGGTAC
    CGAGATCTTG CTGCACTTCT TCAGGATGGC ATCCCTGCCT TCTCTTCCAA GAATGGAAAG
    AACGCTATGG ATGAGAGGAC TTCATATGTT AAGACAGCTC TCTGCGAAGG AGGTGTACCA
    CAGAGACCAG TTGTGTTTGT CTCTCGGCCA AAGCTGGTAG ATGATATTAA ACAGAAACTG
    CGCAGCTTAG GAAGTGATCC AGGCTGGGTG ACCGTTTATG GAATGGCAGG TTGTGGAAAG
    ACCGTTTTAA CAGCAGAAGC TTTAAGGGAT CACCAGCTCT TGCGAGGTTG CTTTCCAGGA
    GGAGTTCACT GGATCTCTGT GGGAAAACAG GACAAAGCAG GGCTCCTCAT AAAGCTACAG
    AATCTCTGTA GTAGATTAGA GCATGACTCT ACTCTTCCAC AGAGACCACC ACTTAACATT
    GAGGAGGCCA AAGATCGTCT TCGTTTGCTG ATGCTGCGCA AATACCAGAG GTCTCTTTTG
    GTCCTGGATG ACATTTGGGA TTCCTGGGTA CTAAAAGCAT TTGATAATCA ATGTCAGGTT
    CTTATAACCA GCAGGGATAG GAGTGTGACA GATGCTGTGT CTGGCAATAA ATATGAGATT
    CACGTGGAAA GTGGACTAAC ACATGAGAAA GGACTGGAAG TTTTAGCCCT GTTTGTGAAT
    ATGAAAATAT CAGAACTGCC AGAACAAGCC AGCTGCATTG TAACAGAATG CAAAGGTTCT
    CCTCTTGTGA TATCCTTGAT AGGTGCATTA CTACGAGATT TCCCTAGTCG CTGGGAATAT
    TATCTCAAAC AGCTGCAGAA TAAGCAATTT AAAAGAATAA GAAAGTCTTC ATCTTATGAC
    TATGAAGCCC TTGATGAAGC AATGTCCATA AGTGTAGAGC AACTGAGTAA TGATTTAAAA
    GAATATTATA AAGACCTCTC CATCCTCCCA AAAGATGTTA AAGTGCCTAC TAAGGTGCTC
    TGTATTCTTT GGGATATGGA AACTGAAGAA GTTGAAGATA TACTGCAGGA ATTTGTTAAC
    AAATCACTAT TGTTCTGCGA TCGTAATGGG AATTCATTTC ATTATTATTT ACATGATCTT
    CAACTCGACT TTCTTACAGA GAAGAATTGC AACCAGCTTC AGGAACTACA TAAAAATATA
    GTAAATCAGT ACAAGAGATA TTACAAGCTT CATGTGCCTG TGCTATCCCA AGAGGACTGT
    ATGTACTGGT ATAACTTTCT AGCATATCAC ATGGCTGGTG CCAACATGCA GAAGGAACTC
    CGTGATCTTA TGTTTTCTCT AGATTGGATT AAAGCTAAAA CAGAATTGGT AGGACCTGCT
    CATCTAATTC ATGAATATGT AGAGTATAGT TCAGTCCTGG ATGAAAAGGA TAGCATAATA
    CGTGAGAATT TTCAGGAATT TCTGTCTTTA AATGGGCACC TTCTTGGACG ACAGCCTTTT
    CCTGACATTA CCCAGCTTGG TCTTTGCCAA CCAGAGACAT CAGAGGTGTA TCAGCAAGCC
    AAGCTACGGG CTCAAAGAGA AACGGGAACA TTTTACTTGG AATGGATAAA TAAAAAATCC
    TTGAAAAACC TTTCCCGTCT GGTTGTTCGT CCGCACAGAG ATGCAGTTTA CCATGCCTGC
    TTTTCTAGGG ATAGACAAAG AATAGCATCA TGTGGAGCTG ATAAGACACT TCAGCAGTCT
    GCCTGGAGGG AAGCCAGACA GCTCTCAGGA AAACTAGCAA TCAGCCAGCC ACTAACCTGG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa29641
    Clone ID Related Accession (Same CDS sequence) XM_015289693.2 , XM_015289693.1
    Accession Version XM_015289693.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1980bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product apoptotic protease-activating factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGATGAGA ACAGTAGAAT GTGTTTAATT ACGAATCGTC AGGCACTGGA GAAAGACATC 
    AAAACCTCTT ACATTATGGA TCATATGGTT GCTGACCAAG TCCTGACATT GCAGGAGGAG
    GAGAAAGTGA AACAACAGAA TACACAGAAG GAGCGAGCAG CTATGCTAAT TAATACTCTT
    CTTACCAAAG ACAATAATGC ATATAGATCT TTTTATAATG CACTGCTTCA TGAAGGGTAC
    CGAGATCTTG CTGCACTTCT TCAGGATGGC ATCCCTGCCT TCTCTTCCAA GAATGGAAAG
    AACGCTATGG ATGAGAGGAC TTCATATGTT AAGACAGCTC TCTGCGAAGG AGGTGTACCA
    CAGAGACCAG TTGTGTTTGT CTCTCGGCCA AAGCTGGTAG ATGATATTAA ACAGAAACTG
    CGCAGCTTAG GAAGTGATCC AGGCTGGGTG ACCGTTTATG GAATGGCAGG TTGTGGAAAG
    ACCGTTTTAA CAGCAGAAGC TTTAAGGGAT CACCAGCTCT TGCGAGGTTG CTTTCCAGGA
    GGAGTTCACT GGATCTCTGT GGGAAAACAG GACAAAGCAG GGCTCCTCAT AAAGCTACAG
    AATCTCTGTA GTAGATTAGA GCATGACTCT ACTCTTCCAC AGAGACCACC ACTTAACATT
    GAGGAGGCCA AAGATCGTCT TCGTTTGCTG ATGCTGCGCA AATACCAGAG GTCTCTTTTG
    GTCCTGGATG ACATTTGGGA TTCCTGGGTA CTAAAAGCAT TTGATAATCA ATGTCAGGTT
    CTTATAACCA GCAGGGATAG GAGTGTGACA GATGCTGTGT CTGGCAATAA ATATGAGATT
    CACGTGGAAA GTGGACTAAC ACATGAGAAA GGACTGGAAG TTTTAGCCCT GTTTGTGAAT
    ATGAAAATAT CAGAACTGCC AGAACAAGCC AGCTGCATTG TAACAGAATG CAAAGGTTCT
    CCTCTTGTGA TATCCTTGAT AGGTGCATTA CTACGAGATT TCCCTAGTCG CTGGGAATAT
    TATCTCAAAC AGCTGCAGAA TAAGCAATTT AAAAGAATAA GAAAGTCTTC ATCTTATGAC
    TATGAAGCCC TTGATGAAGC AATGTCCATA AGTGTAGAGC AACTGAGTAA TGATTTAAAA
    GAATATTATA AAGACCTCTC CATCCTCCCA AAAGATGTTA AAGTGCCTAC TAAGGTGCTC
    TGTATTCTTT GGGATATGGA AACTGAAGAA GTTGAAGATA TACTGCAGGA ATTTGTTAAC
    AAATCACTAT TGTTCTGCGA TCGTAATGGG AATTCATTTC ATTATTATTT ACATGATCTT
    CAACTCGACT TTCTTACAGA GAAGAATTGC AACCAGCTTC AGGAACTACA TAAAAATATA
    GTAAATCAGT ACAAGAGATA TTACAAGCTT CATGTGCCTG TGCTATCCCA AGAGGACTGT
    ATGTACTGGT ATAACTTTCT AGCATATCAC ATGGCTGGTG CCAACATGCA GAAGGAACTC
    CGTGATCTTA TGTTTTCTCT AGATTGGATT AAAGCTAAAA CAGAATTGGT AGGACCTGCT
    CATCTAATTC ATGAATATGT AGAGTATAGT TCAGTCCTGG ATGAAAAGGA TAGCATAATA
    CGTGAGAATT TTCAGGAATT TCTGTCTTTA AATGGGCACC TTCTTGGACG ACAGCCTTTT
    CCTGACATTA CCCAGCTTGG TCTTTGCCAA CCAGAGACAT CAGAGGTGTA TCAGCAAGCC
    AAGCTACGGG CTCAAAGAGA AACGGGAACA TTTTACTTGG AATGGATAAA TAAAAAATCC
    TTGAAAAACC TTTCCCGTCT GGTTGTTCGT CCGCACAGAG ATGCAGTTTA CCATGCCTGC
    TTTTCTAGGG ATAGACAAAG AATAGCATCA TGTGGAGCTG ATAAGACACT TCAGTCTGCC
    TGGAGGGAAG CCAGACAGCT CTCAGGAAAA CTAGCAATCA GCCAGCCACT AACCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145179.1
    CDS145..2124
    Translation

    Target ORF information:

    RefSeq Version XM_015289693.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus apoptotic peptidase activating factor 1 (APAF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289693.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGATGAGA ACAGTAGAAT GTGTTTAATT ACGAATCGTC AGGCACTGGA GAAAGACATC 
    AAAACCTCTT ACATTATGGA TCATATGGTT GCTGACCAAG TCCTGACATT GCAGGAGGAG
    GAGAAAGTGA AACAACAGAA TACACAGAAG GAGCGAGCAG CTATGCTAAT TAATACTCTT
    CTTACCAAAG ACAATAATGC ATATAGATCT TTTTATAATG CACTGCTTCA TGAAGGGTAC
    CGAGATCTTG CTGCACTTCT TCAGGATGGC ATCCCTGCCT TCTCTTCCAA GAATGGAAAG
    AACGCTATGG ATGAGAGGAC TTCATATGTT AAGACAGCTC TCTGCGAAGG AGGTGTACCA
    CAGAGACCAG TTGTGTTTGT CTCTCGGCCA AAGCTGGTAG ATGATATTAA ACAGAAACTG
    CGCAGCTTAG GAAGTGATCC AGGCTGGGTG ACCGTTTATG GAATGGCAGG TTGTGGAAAG
    ACCGTTTTAA CAGCAGAAGC TTTAAGGGAT CACCAGCTCT TGCGAGGTTG CTTTCCAGGA
    GGAGTTCACT GGATCTCTGT GGGAAAACAG GACAAAGCAG GGCTCCTCAT AAAGCTACAG
    AATCTCTGTA GTAGATTAGA GCATGACTCT ACTCTTCCAC AGAGACCACC ACTTAACATT
    GAGGAGGCCA AAGATCGTCT TCGTTTGCTG ATGCTGCGCA AATACCAGAG GTCTCTTTTG
    GTCCTGGATG ACATTTGGGA TTCCTGGGTA CTAAAAGCAT TTGATAATCA ATGTCAGGTT
    CTTATAACCA GCAGGGATAG GAGTGTGACA GATGCTGTGT CTGGCAATAA ATATGAGATT
    CACGTGGAAA GTGGACTAAC ACATGAGAAA GGACTGGAAG TTTTAGCCCT GTTTGTGAAT
    ATGAAAATAT CAGAACTGCC AGAACAAGCC AGCTGCATTG TAACAGAATG CAAAGGTTCT
    CCTCTTGTGA TATCCTTGAT AGGTGCATTA CTACGAGATT TCCCTAGTCG CTGGGAATAT
    TATCTCAAAC AGCTGCAGAA TAAGCAATTT AAAAGAATAA GAAAGTCTTC ATCTTATGAC
    TATGAAGCCC TTGATGAAGC AATGTCCATA AGTGTAGAGC AACTGAGTAA TGATTTAAAA
    GAATATTATA AAGACCTCTC CATCCTCCCA AAAGATGTTA AAGTGCCTAC TAAGGTGCTC
    TGTATTCTTT GGGATATGGA AACTGAAGAA GTTGAAGATA TACTGCAGGA ATTTGTTAAC
    AAATCACTAT TGTTCTGCGA TCGTAATGGG AATTCATTTC ATTATTATTT ACATGATCTT
    CAACTCGACT TTCTTACAGA GAAGAATTGC AACCAGCTTC AGGAACTACA TAAAAATATA
    GTAAATCAGT ACAAGAGATA TTACAAGCTT CATGTGCCTG TGCTATCCCA AGAGGACTGT
    ATGTACTGGT ATAACTTTCT AGCATATCAC ATGGCTGGTG CCAACATGCA GAAGGAACTC
    CGTGATCTTA TGTTTTCTCT AGATTGGATT AAAGCTAAAA CAGAATTGGT AGGACCTGCT
    CATCTAATTC ATGAATATGT AGAGTATAGT TCAGTCCTGG ATGAAAAGGA TAGCATAATA
    CGTGAGAATT TTCAGGAATT TCTGTCTTTA AATGGGCACC TTCTTGGACG ACAGCCTTTT
    CCTGACATTA CCCAGCTTGG TCTTTGCCAA CCAGAGACAT CAGAGGTGTA TCAGCAAGCC
    AAGCTACGGG CTCAAAGAGA AACGGGAACA TTTTACTTGG AATGGATAAA TAAAAAATCC
    TTGAAAAACC TTTCCCGTCT GGTTGTTCGT CCGCACAGAG ATGCAGTTTA CCATGCCTGC
    TTTTCTAGGG ATAGACAAAG AATAGCATCA TGTGGAGCTG ATAAGACACT TCAGTCTGCC
    TGGAGGGAAG CCAGACAGCT CTCAGGAAAA CTAGCAATCA GCCAGCCACT AACCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa29641
    Clone ID Related Accession (Same CDS sequence) XM_015289693.2 , XM_015289693.1
    Accession Version XM_015289693.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1980bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product apoptotic protease-activating factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGATGAGA ACAGTAGAAT GTGTTTAATT ACGAATCGTC AGGCACTGGA GAAAGACATC 
    AAAACCTCTT ACATTATGGA TCATATGGTT GCTGACCAAG TCCTGACATT GCAGGAGGAG
    GAGAAAGTGA AACAACAGAA TACACAGAAG GAGCGAGCAG CTATGCTAAT TAATACTCTT
    CTTACCAAAG ACAATAATGC ATATAGATCT TTTTATAATG CACTGCTTCA TGAAGGGTAC
    CGAGATCTTG CTGCACTTCT TCAGGATGGC ATCCCTGCCT TCTCTTCCAA GAATGGAAAG
    AACGCTATGG ATGAGAGGAC TTCATATGTT AAGACAGCTC TCTGCGAAGG AGGTGTACCA
    CAGAGACCAG TTGTGTTTGT CTCTCGGCCA AAGCTGGTAG ATGATATTAA ACAGAAACTG
    CGCAGCTTAG GAAGTGATCC AGGCTGGGTG ACCGTTTATG GAATGGCAGG TTGTGGAAAG
    ACCGTTTTAA CAGCAGAAGC TTTAAGGGAT CACCAGCTCT TGCGAGGTTG CTTTCCAGGA
    GGAGTTCACT GGATCTCTGT GGGAAAACAG GACAAAGCAG GGCTCCTCAT AAAGCTACAG
    AATCTCTGTA GTAGATTAGA GCATGACTCT ACTCTTCCAC AGAGACCACC ACTTAACATT
    GAGGAGGCCA AAGATCGTCT TCGTTTGCTG ATGCTGCGCA AATACCAGAG GTCTCTTTTG
    GTCCTGGATG ACATTTGGGA TTCCTGGGTA CTAAAAGCAT TTGATAATCA ATGTCAGGTT
    CTTATAACCA GCAGGGATAG GAGTGTGACA GATGCTGTGT CTGGCAATAA ATATGAGATT
    CACGTGGAAA GTGGACTAAC ACATGAGAAA GGACTGGAAG TTTTAGCCCT GTTTGTGAAT
    ATGAAAATAT CAGAACTGCC AGAACAAGCC AGCTGCATTG TAACAGAATG CAAAGGTTCT
    CCTCTTGTGA TATCCTTGAT AGGTGCATTA CTACGAGATT TCCCTAGTCG CTGGGAATAT
    TATCTCAAAC AGCTGCAGAA TAAGCAATTT AAAAGAATAA GAAAGTCTTC ATCTTATGAC
    TATGAAGCCC TTGATGAAGC AATGTCCATA AGTGTAGAGC AACTGAGTAA TGATTTAAAA
    GAATATTATA AAGACCTCTC CATCCTCCCA AAAGATGTTA AAGTGCCTAC TAAGGTGCTC
    TGTATTCTTT GGGATATGGA AACTGAAGAA GTTGAAGATA TACTGCAGGA ATTTGTTAAC
    AAATCACTAT TGTTCTGCGA TCGTAATGGG AATTCATTTC ATTATTATTT ACATGATCTT
    CAACTCGACT TTCTTACAGA GAAGAATTGC AACCAGCTTC AGGAACTACA TAAAAATATA
    GTAAATCAGT ACAAGAGATA TTACAAGCTT CATGTGCCTG TGCTATCCCA AGAGGACTGT
    ATGTACTGGT ATAACTTTCT AGCATATCAC ATGGCTGGTG CCAACATGCA GAAGGAACTC
    CGTGATCTTA TGTTTTCTCT AGATTGGATT AAAGCTAAAA CAGAATTGGT AGGACCTGCT
    CATCTAATTC ATGAATATGT AGAGTATAGT TCAGTCCTGG ATGAAAAGGA TAGCATAATA
    CGTGAGAATT TTCAGGAATT TCTGTCTTTA AATGGGCACC TTCTTGGACG ACAGCCTTTT
    CCTGACATTA CCCAGCTTGG TCTTTGCCAA CCAGAGACAT CAGAGGTGTA TCAGCAAGCC
    AAGCTACGGG CTCAAAGAGA AACGGGAACA TTTTACTTGG AATGGATAAA TAAAAAATCC
    TTGAAAAACC TTTCCCGTCT GGTTGTTCGT CCGCACAGAG ATGCAGTTTA CCATGCCTGC
    TTTTCTAGGG ATAGACAAAG AATAGCATCA TGTGGAGCTG ATAAGACACT TCAGTCTGCC
    TGGAGGGAAG CCAGACAGCT CTCAGGAAAA CTAGCAATCA GCCAGCCACT AACCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015145179.1
    CDS123..2102
    Translation

    Target ORF information:

    RefSeq Version XM_015289693.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus apoptotic peptidase activating factor 1 (APAF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289693.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGATGAGA ACAGTAGAAT GTGTTTAATT ACGAATCGTC AGGCACTGGA GAAAGACATC 
    AAAACCTCTT ACATTATGGA TCATATGGTT GCTGACCAAG TCCTGACATT GCAGGAGGAG
    GAGAAAGTGA AACAACAGAA TACACAGAAG GAGCGAGCAG CTATGCTAAT TAATACTCTT
    CTTACCAAAG ACAATAATGC ATATAGATCT TTTTATAATG CACTGCTTCA TGAAGGGTAC
    CGAGATCTTG CTGCACTTCT TCAGGATGGC ATCCCTGCCT TCTCTTCCAA GAATGGAAAG
    AACGCTATGG ATGAGAGGAC TTCATATGTT AAGACAGCTC TCTGCGAAGG AGGTGTACCA
    CAGAGACCAG TTGTGTTTGT CTCTCGGCCA AAGCTGGTAG ATGATATTAA ACAGAAACTG
    CGCAGCTTAG GAAGTGATCC AGGCTGGGTG ACCGTTTATG GAATGGCAGG TTGTGGAAAG
    ACCGTTTTAA CAGCAGAAGC TTTAAGGGAT CACCAGCTCT TGCGAGGTTG CTTTCCAGGA
    GGAGTTCACT GGATCTCTGT GGGAAAACAG GACAAAGCAG GGCTCCTCAT AAAGCTACAG
    AATCTCTGTA GTAGATTAGA GCATGACTCT ACTCTTCCAC AGAGACCACC ACTTAACATT
    GAGGAGGCCA AAGATCGTCT TCGTTTGCTG ATGCTGCGCA AATACCAGAG GTCTCTTTTG
    GTCCTGGATG ACATTTGGGA TTCCTGGGTA CTAAAAGCAT TTGATAATCA ATGTCAGGTT
    CTTATAACCA GCAGGGATAG GAGTGTGACA GATGCTGTGT CTGGCAATAA ATATGAGATT
    CACGTGGAAA GTGGACTAAC ACATGAGAAA GGACTGGAAG TTTTAGCCCT GTTTGTGAAT
    ATGAAAATAT CAGAACTGCC AGAACAAGCC AGCTGCATTG TAACAGAATG CAAAGGTTCT
    CCTCTTGTGA TATCCTTGAT AGGTGCATTA CTACGAGATT TCCCTAGTCG CTGGGAATAT
    TATCTCAAAC AGCTGCAGAA TAAGCAATTT AAAAGAATAA GAAAGTCTTC ATCTTATGAC
    TATGAAGCCC TTGATGAAGC AATGTCCATA AGTGTAGAGC AACTGAGTAA TGATTTAAAA
    GAATATTATA AAGACCTCTC CATCCTCCCA AAAGATGTTA AAGTGCCTAC TAAGGTGCTC
    TGTATTCTTT GGGATATGGA AACTGAAGAA GTTGAAGATA TACTGCAGGA ATTTGTTAAC
    AAATCACTAT TGTTCTGCGA TCGTAATGGG AATTCATTTC ATTATTATTT ACATGATCTT
    CAACTCGACT TTCTTACAGA GAAGAATTGC AACCAGCTTC AGGAACTACA TAAAAATATA
    GTAAATCAGT ACAAGAGATA TTACAAGCTT CATGTGCCTG TGCTATCCCA AGAGGACTGT
    ATGTACTGGT ATAACTTTCT AGCATATCAC ATGGCTGGTG CCAACATGCA GAAGGAACTC
    CGTGATCTTA TGTTTTCTCT AGATTGGATT AAAGCTAAAA CAGAATTGGT AGGACCTGCT
    CATCTAATTC ATGAATATGT AGAGTATAGT TCAGTCCTGG ATGAAAAGGA TAGCATAATA
    CGTGAGAATT TTCAGGAATT TCTGTCTTTA AATGGGCACC TTCTTGGACG ACAGCCTTTT
    CCTGACATTA CCCAGCTTGG TCTTTGCCAA CCAGAGACAT CAGAGGTGTA TCAGCAAGCC
    AAGCTACGGG CTCAAAGAGA AACGGGAACA TTTTACTTGG AATGGATAAA TAAAAAATCC
    TTGAAAAACC TTTCCCGTCT GGTTGTTCGT CCGCACAGAG ATGCAGTTTA CCATGCCTGC
    TTTTCTAGGG ATAGACAAAG AATAGCATCA TGTGGAGCTG ATAAGACACT TCAGTCTGCC
    TGGAGGGAAG CCAGACAGCT CTCAGGAAAA CTAGCAATCA GCCAGCCACT AACCTGGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa01652
    Clone ID Related Accession (Same CDS sequence) XM_416167.5 , XM_416167.6
    Accession Version XM_416167.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product apoptotic protease-activating factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455714.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 4, 2016 this sequence version replaced XM_416167.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGAGA ACAGTAGAAT GTGTTTAATT ACGAATCGTC AGGCACTGGA GAAAGACATC 
    AAAACCTCTT ACATTATGGA TCATATGGTT GCTGACCAAG TCCTGACATT GCAGGAGGAG
    GAGAAAGTGA AACAACAGAA TACACAGAAG GAGCGAGCAG CTATGCTAAT TAATACTCTT
    CTTACCAAAG ACAATAATGC ATATAGATCT TTTTATAATG CACTGCTTCA TGAAGGGTAC
    CGAGATCTTG CTGCACTTCT TCAGGATGGC ATCCCTGCCT TCTCTTCCAA GAATGGAAAG
    AACGCTATGG ATGAGAGGAC TTCATATGTT AAGACAGCTC TCTGCGAAGG AGGTGTACCA
    CAGAGACCAG TTGTGTTTGT CTCTCGGCCA AAGCTGGTAG ATGATATTAA ACAGAAACTG
    CGCAGCTTAG GAAGTGATCC AGGCTGGGTG ACCGTTTATG GAATGGCAGG TTGTGGAAAG
    ACCGTTTTAA CAGCAGAAGC TTTAAGGGAT CACCAGCTCT TGCGAGGTTG CTTTCCAGGA
    GGAGTTCACT GGATCTCTGT GGGAAAACAG GACAAAGCAG GGCTCCTCAT AAAGCTACAG
    AATCTCTGTA GTAGATTAGA GCATGACTCT ACTCTTCCAC AGAGACCACC ACTTAACATT
    GAGGAGGCCA AAGATCGTCT TCGTTTGCTG ATGCTGCGCA AATACCAGAG GTCTCTTTTG
    GTCCTGGATG ACATTTGGGA TTCCTGGGTA CTAAAAGCAT TTGATAATCA ATGTCAGGTT
    CTTATAACCA GCAGGGATAG GAGTGTGACA GATGCTGTGT CTGGCAATAA ATATGAGATT
    CACGTGGAAA GTGGACTAAC ACATGAGAAA GGACTGGAAG TTTTAGCCCT GTTTGTGAAT
    ATGAAAATAT CAGAACTGCC AGAACAAGCC AGCTGCATTG TAACAGAATG CAAAGGTTCT
    CCTCTTGTGA TATCCTTGAT AGGTGCATTA CTACGAGATT TCCCTAGTCG CTGGGAATAT
    TATCTCAAAC AGCTGCAGAA TAAGCAATTT AAAAGAATAA GAAAGTCTTC ATCTTATGAC
    TATGAAGCCC TTGATGAAGC AATGTCCATA AGTGTAGAGC AACTGAGTAA TGATTTAAAA
    GAATATTATA AAGACCTCTC CATCCTCCCA AAAGATGTTA AAGTGCCTAC TAAGGTGCTC
    TGTATTCTTT GGGATATGGA AACTGAAGAA GTTGAAGATA TACTGCAGGA ATTTGTTAAC
    AAATCACTAT TGTTCTGCGA TCGTAATGGG AATTCATTTC ATTATTATTT ACATGATCTT
    CAACTCGACT TTCTTACAGA GAAGAATTGC AACCAGCTTC AGGAACTACA TAAAAATATA
    GTAAATCAGT ACAAGAGATA TTACAAGCTT CATGTGCCTG TGCTATCCCA AGAGGACTGT
    ATGTACTGGT ATAACTTTCT AGCATATCAC ATGGCTGGTG CCAACATGCA GAAGGAACTC
    CGTGATCTTA TGTTTTCTCT AGATTGGATT AAAGCTAAAA CAGAATTGGT AGGACCTGCT
    CATCTAATTC ATGAATATGT AGAGTATAGT TCAGTCCTGG ATGAAAAGGA TAGCATAATA
    CGTGAGAATT TTCAGGAATT TCTGTCTTTA AATGGGCACC TTCTTGGACG ACAGCCTTTT
    CCTGACATTA CCCAGCTTGG TCTTTGCCAA CCAGAGACAT CAGAGGTGTA TCAGCAAGCC
    AAGCTACGGG CTCAAAGAGA AACGGGAACA TTTTACTTGG AATGGATAAA TAAAAAATCC
    TTGAAAAACC TTTCCCGTCT GGTTGTTCGT CCGCACAGAG ATGCAGTTTA CCATGCCTGC
    TTTTCTAGGG ATAGACAAAG AATAGCATCA TGTGGAGCTG ATAAGACACT TCAGGTGTTT
    AAAGCAGAAA GCGGAGAGAG ATTGCTGGAG ATAAGTGCTC ATGATGATGA AATACTTTGC
    TGCACTTTTT CTGCAGATGA TAAATATGTA GCAACTTGTT CAGCTGATAA AAAAGTGAAG
    ATCTGGAATT CAAGAACGGG CAAGTGTTTG TACGTATATG AAGAACACAC AGAGCAGGTC
    AATTGCTGCC AGTTCAATAA CAGAAGGGGC CAGTATCTTT TAGCCACCTG CTCAGATGAC
    ACTTATATCA AACTTTGGGA TTTGAAGGAT AAGTATTGTA GGAATACGAT GTTTGGTCAT
    GTAAATGCTG TCAGTCACTG TAGATTTTCA CCAAATGATG ACTATATTGC CAGCTGCTCA
    ACAGATGGAA CAGTGAAGCT TTGGAATGTG CATTCAGCAA ATGAACTGAG AACTATTGAG
    ATAAAAGATT TCTTCAGGAA TGCAGATGAG CAGCAAGATG ACGTGGAGGT TTTAGTGAAA
    TGTTGCTCTT GGTCCAGAAG TAGTGATACA ATTTTGGTCG TAGCCAAAAA TAAACTTTTG
    CTTTTTGATG TTACAACAAG TAATCTGTTA ACACAAGTCA TCATAAGTCA TCACAGTACA
    ATCCAATATT GCGACTTCTG CGCTGGGGAT GAGTTAGTAG CAGTTGCTTT GTCTCATTGT
    TCTATTGAGT TATGGAATAT TAAATCCTTA TCAAAAGTAG CAGATTGCAG AGGACACACG
    AGCTGGGTTC ACTGTGTGAC GTTTTCCTCA GATGGATCTT TATTTCTGAC ATCTTCTGAG
    GATCAGACAA TACGGATTTG GGAAACAAAC AAGGTGTGCA AGTCTTCTGA TGCCGTGCTA
    AAAAGTGAAC TGGATGTTGT GTTTCACAAT GGTGAAGTCA TGATTTTAGC AATTTACAAT
    CAGAAGCATA TACAACTCAT TAATGGAAAT ACAGACAGCA TTATTATGCA GACAGAACCT
    CAGGAATCCC TTATTTGCTG CTGTTGCTTA AGTGAAGATC TTAAATTTGC AGCATTTGGG
    CAGGAGAATG GAGCAATAAA GGTACTACGG TTGTTGGATG GGAGAGTTCT GACATCCCGG
    GAGGCCTACA GGACATCTGT GCAACATTGT CGATTTACCA CTGATTGTCA GACTCTGATT
    TCAAGTGCCC ATGATGCAGT CATACAGGTC TGGAATTGGC AGTTGAACGA GTGTGTGTTT
    CTAAAAGGGC ATAAGGAAGC AATCAAAGAC TTTGCATTTC TGGAGAATTC AAAACTTCTT
    TCCTGGTCAT TTGATGGAAC AGTTAAGGTT TGGAATATCA CCACTGGAAA AACAGAAAAA
    GATTTTGCTT GCCACAGAGG TGCTGTTCTT TCTTGTGCTG TCTCACCTGA TGGTAGCAGG
    TTTTCATCTA CTTCTGCTGA CAAAACTGCA AAGATATGGA GCTTTGAAAG TTCATCTGTT
    CTTCATGAAC TGAAAGGTCA TGAAGCCTGT GTGCGGTGCT CTGCTTTCTC TCCTAGTAAC
    AAATTACTGG CCACTGGAGA TGACAAAGGA GATATAAGGA TTTGGGACAT TTTAACAGGT
    GAATTACTTC ACTTCTGCTC CTCAGTTACT GTGGATGAAG AAGAACCAAC ACATGGTGGC
    TGGGTAACAG ACCTCTCGTT TTCTCCTGAC AGTAAAATGC TTGTGTCATC TGGAGGCTAT
    CTTAAGTGGT GGGATGTAAC TACTGGAGAA TCCTTGCAAA CCTTCTACAC GAACGGAACA
    AACCTCAAGT CAATACACGT GTCCCCTAAT TTTGACGTGT ACGTGACTGT TGATAATCTT
    GGTATTCTTT ATGTATTACG GAAGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_416167.3
    CDS123..3872
    Translation

    Target ORF information:

    RefSeq Version XM_416167.5
    Organism Gallus gallus(chicken)
    Definition Gallus gallus apoptotic peptidase activating factor 1 (APAF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_416167.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGAGA ACAGTAGAAT GTGTTTAATT ACGAATCGTC AGGCACTGGA GAAAGACATC 
    AAAACCTCTT ACATTATGGA TCATATGGTT GCTGACCAAG TCCTGACATT GCAGGAGGAG
    GAGAAAGTGA AACAACAGAA TACACAGAAG GAGCGAGCAG CTATGCTAAT TAATACTCTT
    CTTACCAAAG ACAATAATGC ATATAGATCT TTTTATAATG CACTGCTTCA TGAAGGGTAC
    CGAGATCTTG CTGCACTTCT TCAGGATGGC ATCCCTGCCT TCTCTTCCAA GAATGGAAAG
    AACGCTATGG ATGAGAGGAC TTCATATGTT AAGACAGCTC TCTGCGAAGG AGGTGTACCA
    CAGAGACCAG TTGTGTTTGT CTCTCGGCCA AAGCTGGTAG ATGATATTAA ACAGAAACTG
    CGCAGCTTAG GAAGTGATCC AGGCTGGGTG ACCGTTTATG GAATGGCAGG TTGTGGAAAG
    ACCGTTTTAA CAGCAGAAGC TTTAAGGGAT CACCAGCTCT TGCGAGGTTG CTTTCCAGGA
    GGAGTTCACT GGATCTCTGT GGGAAAACAG GACAAAGCAG GGCTCCTCAT AAAGCTACAG
    AATCTCTGTA GTAGATTAGA GCATGACTCT ACTCTTCCAC AGAGACCACC ACTTAACATT
    GAGGAGGCCA AAGATCGTCT TCGTTTGCTG ATGCTGCGCA AATACCAGAG GTCTCTTTTG
    GTCCTGGATG ACATTTGGGA TTCCTGGGTA CTAAAAGCAT TTGATAATCA ATGTCAGGTT
    CTTATAACCA GCAGGGATAG GAGTGTGACA GATGCTGTGT CTGGCAATAA ATATGAGATT
    CACGTGGAAA GTGGACTAAC ACATGAGAAA GGACTGGAAG TTTTAGCCCT GTTTGTGAAT
    ATGAAAATAT CAGAACTGCC AGAACAAGCC AGCTGCATTG TAACAGAATG CAAAGGTTCT
    CCTCTTGTGA TATCCTTGAT AGGTGCATTA CTACGAGATT TCCCTAGTCG CTGGGAATAT
    TATCTCAAAC AGCTGCAGAA TAAGCAATTT AAAAGAATAA GAAAGTCTTC ATCTTATGAC
    TATGAAGCCC TTGATGAAGC AATGTCCATA AGTGTAGAGC AACTGAGTAA TGATTTAAAA
    GAATATTATA AAGACCTCTC CATCCTCCCA AAAGATGTTA AAGTGCCTAC TAAGGTGCTC
    TGTATTCTTT GGGATATGGA AACTGAAGAA GTTGAAGATA TACTGCAGGA ATTTGTTAAC
    AAATCACTAT TGTTCTGCGA TCGTAATGGG AATTCATTTC ATTATTATTT ACATGATCTT
    CAACTCGACT TTCTTACAGA GAAGAATTGC AACCAGCTTC AGGAACTACA TAAAAATATA
    GTAAATCAGT ACAAGAGATA TTACAAGCTT CATGTGCCTG TGCTATCCCA AGAGGACTGT
    ATGTACTGGT ATAACTTTCT AGCATATCAC ATGGCTGGTG CCAACATGCA GAAGGAACTC
    CGTGATCTTA TGTTTTCTCT AGATTGGATT AAAGCTAAAA CAGAATTGGT AGGACCTGCT
    CATCTAATTC ATGAATATGT AGAGTATAGT TCAGTCCTGG ATGAAAAGGA TAGCATAATA
    CGTGAGAATT TTCAGGAATT TCTGTCTTTA AATGGGCACC TTCTTGGACG ACAGCCTTTT
    CCTGACATTA CCCAGCTTGG TCTTTGCCAA CCAGAGACAT CAGAGGTGTA TCAGCAAGCC
    AAGCTACGGG CTCAAAGAGA AACGGGAACA TTTTACTTGG AATGGATAAA TAAAAAATCC
    TTGAAAAACC TTTCCCGTCT GGTTGTTCGT CCGCACAGAG ATGCAGTTTA CCATGCCTGC
    TTTTCTAGGG ATAGACAAAG AATAGCATCA TGTGGAGCTG ATAAGACACT TCAGGTGTTT
    AAAGCAGAAA GCGGAGAGAG ATTGCTGGAG ATAAGTGCTC ATGATGATGA AATACTTTGC
    TGCACTTTTT CTGCAGATGA TAAATATGTA GCAACTTGTT CAGCTGATAA AAAAGTGAAG
    ATCTGGAATT CAAGAACGGG CAAGTGTTTG TACGTATATG AAGAACACAC AGAGCAGGTC
    AATTGCTGCC AGTTCAATAA CAGAAGGGGC CAGTATCTTT TAGCCACCTG CTCAGATGAC
    ACTTATATCA AACTTTGGGA TTTGAAGGAT AAGTATTGTA GGAATACGAT GTTTGGTCAT
    GTAAATGCTG TCAGTCACTG TAGATTTTCA CCAAATGATG ACTATATTGC CAGCTGCTCA
    ACAGATGGAA CAGTGAAGCT TTGGAATGTG CATTCAGCAA ATGAACTGAG AACTATTGAG
    ATAAAAGATT TCTTCAGGAA TGCAGATGAG CAGCAAGATG ACGTGGAGGT TTTAGTGAAA
    TGTTGCTCTT GGTCCAGAAG TAGTGATACA ATTTTGGTCG TAGCCAAAAA TAAACTTTTG
    CTTTTTGATG TTACAACAAG TAATCTGTTA ACACAAGTCA TCATAAGTCA TCACAGTACA
    ATCCAATATT GCGACTTCTG CGCTGGGGAT GAGTTAGTAG CAGTTGCTTT GTCTCATTGT
    TCTATTGAGT TATGGAATAT TAAATCCTTA TCAAAAGTAG CAGATTGCAG AGGACACACG
    AGCTGGGTTC ACTGTGTGAC GTTTTCCTCA GATGGATCTT TATTTCTGAC ATCTTCTGAG
    GATCAGACAA TACGGATTTG GGAAACAAAC AAGGTGTGCA AGTCTTCTGA TGCCGTGCTA
    AAAAGTGAAC TGGATGTTGT GTTTCACAAT GGTGAAGTCA TGATTTTAGC AATTTACAAT
    CAGAAGCATA TACAACTCAT TAATGGAAAT ACAGACAGCA TTATTATGCA GACAGAACCT
    CAGGAATCCC TTATTTGCTG CTGTTGCTTA AGTGAAGATC TTAAATTTGC AGCATTTGGG
    CAGGAGAATG GAGCAATAAA GGTACTACGG TTGTTGGATG GGAGAGTTCT GACATCCCGG
    GAGGCCTACA GGACATCTGT GCAACATTGT CGATTTACCA CTGATTGTCA GACTCTGATT
    TCAAGTGCCC ATGATGCAGT CATACAGGTC TGGAATTGGC AGTTGAACGA GTGTGTGTTT
    CTAAAAGGGC ATAAGGAAGC AATCAAAGAC TTTGCATTTC TGGAGAATTC AAAACTTCTT
    TCCTGGTCAT TTGATGGAAC AGTTAAGGTT TGGAATATCA CCACTGGAAA AACAGAAAAA
    GATTTTGCTT GCCACAGAGG TGCTGTTCTT TCTTGTGCTG TCTCACCTGA TGGTAGCAGG
    TTTTCATCTA CTTCTGCTGA CAAAACTGCA AAGATATGGA GCTTTGAAAG TTCATCTGTT
    CTTCATGAAC TGAAAGGTCA TGAAGCCTGT GTGCGGTGCT CTGCTTTCTC TCCTAGTAAC
    AAATTACTGG CCACTGGAGA TGACAAAGGA GATATAAGGA TTTGGGACAT TTTAACAGGT
    GAATTACTTC ACTTCTGCTC CTCAGTTACT GTGGATGAAG AAGAACCAAC ACATGGTGGC
    TGGGTAACAG ACCTCTCGTT TTCTCCTGAC AGTAAAATGC TTGTGTCATC TGGAGGCTAT
    CTTAAGTGGT GGGATGTAAC TACTGGAGAA TCCTTGCAAA CCTTCTACAC GAACGGAACA
    AACCTCAAGT CAATACACGT GTCCCCTAAT TTTGACGTGT ACGTGACTGT TGATAATCTT
    GGTATTCTTT ATGTATTACG GAAGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa01652
    Clone ID Related Accession (Same CDS sequence) XM_416167.5 , XM_416167.6
    Accession Version XM_416167.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3750bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product apoptotic protease-activating factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006088.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_416167.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGAGA ACAGTAGAAT GTGTTTAATT ACGAATCGTC AGGCACTGGA GAAAGACATC 
    AAAACCTCTT ACATTATGGA TCATATGGTT GCTGACCAAG TCCTGACATT GCAGGAGGAG
    GAGAAAGTGA AACAACAGAA TACACAGAAG GAGCGAGCAG CTATGCTAAT TAATACTCTT
    CTTACCAAAG ACAATAATGC ATATAGATCT TTTTATAATG CACTGCTTCA TGAAGGGTAC
    CGAGATCTTG CTGCACTTCT TCAGGATGGC ATCCCTGCCT TCTCTTCCAA GAATGGAAAG
    AACGCTATGG ATGAGAGGAC TTCATATGTT AAGACAGCTC TCTGCGAAGG AGGTGTACCA
    CAGAGACCAG TTGTGTTTGT CTCTCGGCCA AAGCTGGTAG ATGATATTAA ACAGAAACTG
    CGCAGCTTAG GAAGTGATCC AGGCTGGGTG ACCGTTTATG GAATGGCAGG TTGTGGAAAG
    ACCGTTTTAA CAGCAGAAGC TTTAAGGGAT CACCAGCTCT TGCGAGGTTG CTTTCCAGGA
    GGAGTTCACT GGATCTCTGT GGGAAAACAG GACAAAGCAG GGCTCCTCAT AAAGCTACAG
    AATCTCTGTA GTAGATTAGA GCATGACTCT ACTCTTCCAC AGAGACCACC ACTTAACATT
    GAGGAGGCCA AAGATCGTCT TCGTTTGCTG ATGCTGCGCA AATACCAGAG GTCTCTTTTG
    GTCCTGGATG ACATTTGGGA TTCCTGGGTA CTAAAAGCAT TTGATAATCA ATGTCAGGTT
    CTTATAACCA GCAGGGATAG GAGTGTGACA GATGCTGTGT CTGGCAATAA ATATGAGATT
    CACGTGGAAA GTGGACTAAC ACATGAGAAA GGACTGGAAG TTTTAGCCCT GTTTGTGAAT
    ATGAAAATAT CAGAACTGCC AGAACAAGCC AGCTGCATTG TAACAGAATG CAAAGGTTCT
    CCTCTTGTGA TATCCTTGAT AGGTGCATTA CTACGAGATT TCCCTAGTCG CTGGGAATAT
    TATCTCAAAC AGCTGCAGAA TAAGCAATTT AAAAGAATAA GAAAGTCTTC ATCTTATGAC
    TATGAAGCCC TTGATGAAGC AATGTCCATA AGTGTAGAGC AACTGAGTAA TGATTTAAAA
    GAATATTATA AAGACCTCTC CATCCTCCCA AAAGATGTTA AAGTGCCTAC TAAGGTGCTC
    TGTATTCTTT GGGATATGGA AACTGAAGAA GTTGAAGATA TACTGCAGGA ATTTGTTAAC
    AAATCACTAT TGTTCTGCGA TCGTAATGGG AATTCATTTC ATTATTATTT ACATGATCTT
    CAACTCGACT TTCTTACAGA GAAGAATTGC AACCAGCTTC AGGAACTACA TAAAAATATA
    GTAAATCAGT ACAAGAGATA TTACAAGCTT CATGTGCCTG TGCTATCCCA AGAGGACTGT
    ATGTACTGGT ATAACTTTCT AGCATATCAC ATGGCTGGTG CCAACATGCA GAAGGAACTC
    CGTGATCTTA TGTTTTCTCT AGATTGGATT AAAGCTAAAA CAGAATTGGT AGGACCTGCT
    CATCTAATTC ATGAATATGT AGAGTATAGT TCAGTCCTGG ATGAAAAGGA TAGCATAATA
    CGTGAGAATT TTCAGGAATT TCTGTCTTTA AATGGGCACC TTCTTGGACG ACAGCCTTTT
    CCTGACATTA CCCAGCTTGG TCTTTGCCAA CCAGAGACAT CAGAGGTGTA TCAGCAAGCC
    AAGCTACGGG CTCAAAGAGA AACGGGAACA TTTTACTTGG AATGGATAAA TAAAAAATCC
    TTGAAAAACC TTTCCCGTCT GGTTGTTCGT CCGCACAGAG ATGCAGTTTA CCATGCCTGC
    TTTTCTAGGG ATAGACAAAG AATAGCATCA TGTGGAGCTG ATAAGACACT TCAGGTGTTT
    AAAGCAGAAA GCGGAGAGAG ATTGCTGGAG ATAAGTGCTC ATGATGATGA AATACTTTGC
    TGCACTTTTT CTGCAGATGA TAAATATGTA GCAACTTGTT CAGCTGATAA AAAAGTGAAG
    ATCTGGAATT CAAGAACGGG CAAGTGTTTG TACGTATATG AAGAACACAC AGAGCAGGTC
    AATTGCTGCC AGTTCAATAA CAGAAGGGGC CAGTATCTTT TAGCCACCTG CTCAGATGAC
    ACTTATATCA AACTTTGGGA TTTGAAGGAT AAGTATTGTA GGAATACGAT GTTTGGTCAT
    GTAAATGCTG TCAGTCACTG TAGATTTTCA CCAAATGATG ACTATATTGC CAGCTGCTCA
    ACAGATGGAA CAGTGAAGCT TTGGAATGTG CATTCAGCAA ATGAACTGAG AACTATTGAG
    ATAAAAGATT TCTTCAGGAA TGCAGATGAG CAGCAAGATG ACGTGGAGGT TTTAGTGAAA
    TGTTGCTCTT GGTCCAGAAG TAGTGATACA ATTTTGGTCG TAGCCAAAAA TAAACTTTTG
    CTTTTTGATG TTACAACAAG TAATCTGTTA ACACAAGTCA TCATAAGTCA TCACAGTACA
    ATCCAATATT GCGACTTCTG CGCTGGGGAT GAGTTAGTAG CAGTTGCTTT GTCTCATTGT
    TCTATTGAGT TATGGAATAT TAAATCCTTA TCAAAAGTAG CAGATTGCAG AGGACACACG
    AGCTGGGTTC ACTGTGTGAC GTTTTCCTCA GATGGATCTT TATTTCTGAC ATCTTCTGAG
    GATCAGACAA TACGGATTTG GGAAACAAAC AAGGTGTGCA AGTCTTCTGA TGCCGTGCTA
    AAAAGTGAAC TGGATGTTGT GTTTCACAAT GGTGAAGTCA TGATTTTAGC AATTTACAAT
    CAGAAGCATA TACAACTCAT TAATGGAAAT ACAGACAGCA TTATTATGCA GACAGAACCT
    CAGGAATCCC TTATTTGCTG CTGTTGCTTA AGTGAAGATC TTAAATTTGC AGCATTTGGG
    CAGGAGAATG GAGCAATAAA GGTACTACGG TTGTTGGATG GGAGAGTTCT GACATCCCGG
    GAGGCCTACA GGACATCTGT GCAACATTGT CGATTTACCA CTGATTGTCA GACTCTGATT
    TCAAGTGCCC ATGATGCAGT CATACAGGTC TGGAATTGGC AGTTGAACGA GTGTGTGTTT
    CTAAAAGGGC ATAAGGAAGC AATCAAAGAC TTTGCATTTC TGGAGAATTC AAAACTTCTT
    TCCTGGTCAT TTGATGGAAC AGTTAAGGTT TGGAATATCA CCACTGGAAA AACAGAAAAA
    GATTTTGCTT GCCACAGAGG TGCTGTTCTT TCTTGTGCTG TCTCACCTGA TGGTAGCAGG
    TTTTCATCTA CTTCTGCTGA CAAAACTGCA AAGATATGGA GCTTTGAAAG TTCATCTGTT
    CTTCATGAAC TGAAAGGTCA TGAAGCCTGT GTGCGGTGCT CTGCTTTCTC TCCTAGTAAC
    AAATTACTGG CCACTGGAGA TGACAAAGGA GATATAAGGA TTTGGGACAT TTTAACAGGT
    GAATTACTTC ACTTCTGCTC CTCAGTTACT GTGGATGAAG AAGAACCAAC ACATGGTGGC
    TGGGTAACAG ACCTCTCGTT TTCTCCTGAC AGTAAAATGC TTGTGTCATC TGGAGGCTAT
    CTTAAGTGGT GGGATGTAAC TACTGGAGAA TCCTTGCAAA CCTTCTACAC GAACGGAACA
    AACCTCAAGT CAATACACGT GTCCCCTAAT TTTGACGTGT ACGTGACTGT TGATAATCTT
    GGTATTCTTT ATGTATTACG GAAGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_416167.3
    CDS145..3894
    Translation

    Target ORF information:

    RefSeq Version XM_416167.6
    Organism Gallus gallus(chicken)
    Definition Gallus gallus apoptotic peptidase activating factor 1 (APAF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_416167.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATGAGA ACAGTAGAAT GTGTTTAATT ACGAATCGTC AGGCACTGGA GAAAGACATC 
    AAAACCTCTT ACATTATGGA TCATATGGTT GCTGACCAAG TCCTGACATT GCAGGAGGAG
    GAGAAAGTGA AACAACAGAA TACACAGAAG GAGCGAGCAG CTATGCTAAT TAATACTCTT
    CTTACCAAAG ACAATAATGC ATATAGATCT TTTTATAATG CACTGCTTCA TGAAGGGTAC
    CGAGATCTTG CTGCACTTCT TCAGGATGGC ATCCCTGCCT TCTCTTCCAA GAATGGAAAG
    AACGCTATGG ATGAGAGGAC TTCATATGTT AAGACAGCTC TCTGCGAAGG AGGTGTACCA
    CAGAGACCAG TTGTGTTTGT CTCTCGGCCA AAGCTGGTAG ATGATATTAA ACAGAAACTG
    CGCAGCTTAG GAAGTGATCC AGGCTGGGTG ACCGTTTATG GAATGGCAGG TTGTGGAAAG
    ACCGTTTTAA CAGCAGAAGC TTTAAGGGAT CACCAGCTCT TGCGAGGTTG CTTTCCAGGA
    GGAGTTCACT GGATCTCTGT GGGAAAACAG GACAAAGCAG GGCTCCTCAT AAAGCTACAG
    AATCTCTGTA GTAGATTAGA GCATGACTCT ACTCTTCCAC AGAGACCACC ACTTAACATT
    GAGGAGGCCA AAGATCGTCT TCGTTTGCTG ATGCTGCGCA AATACCAGAG GTCTCTTTTG
    GTCCTGGATG ACATTTGGGA TTCCTGGGTA CTAAAAGCAT TTGATAATCA ATGTCAGGTT
    CTTATAACCA GCAGGGATAG GAGTGTGACA GATGCTGTGT CTGGCAATAA ATATGAGATT
    CACGTGGAAA GTGGACTAAC ACATGAGAAA GGACTGGAAG TTTTAGCCCT GTTTGTGAAT
    ATGAAAATAT CAGAACTGCC AGAACAAGCC AGCTGCATTG TAACAGAATG CAAAGGTTCT
    CCTCTTGTGA TATCCTTGAT AGGTGCATTA CTACGAGATT TCCCTAGTCG CTGGGAATAT
    TATCTCAAAC AGCTGCAGAA TAAGCAATTT AAAAGAATAA GAAAGTCTTC ATCTTATGAC
    TATGAAGCCC TTGATGAAGC AATGTCCATA AGTGTAGAGC AACTGAGTAA TGATTTAAAA
    GAATATTATA AAGACCTCTC CATCCTCCCA AAAGATGTTA AAGTGCCTAC TAAGGTGCTC
    TGTATTCTTT GGGATATGGA AACTGAAGAA GTTGAAGATA TACTGCAGGA ATTTGTTAAC
    AAATCACTAT TGTTCTGCGA TCGTAATGGG AATTCATTTC ATTATTATTT ACATGATCTT
    CAACTCGACT TTCTTACAGA GAAGAATTGC AACCAGCTTC AGGAACTACA TAAAAATATA
    GTAAATCAGT ACAAGAGATA TTACAAGCTT CATGTGCCTG TGCTATCCCA AGAGGACTGT
    ATGTACTGGT ATAACTTTCT AGCATATCAC ATGGCTGGTG CCAACATGCA GAAGGAACTC
    CGTGATCTTA TGTTTTCTCT AGATTGGATT AAAGCTAAAA CAGAATTGGT AGGACCTGCT
    CATCTAATTC ATGAATATGT AGAGTATAGT TCAGTCCTGG ATGAAAAGGA TAGCATAATA
    CGTGAGAATT TTCAGGAATT TCTGTCTTTA AATGGGCACC TTCTTGGACG ACAGCCTTTT
    CCTGACATTA CCCAGCTTGG TCTTTGCCAA CCAGAGACAT CAGAGGTGTA TCAGCAAGCC
    AAGCTACGGG CTCAAAGAGA AACGGGAACA TTTTACTTGG AATGGATAAA TAAAAAATCC
    TTGAAAAACC TTTCCCGTCT GGTTGTTCGT CCGCACAGAG ATGCAGTTTA CCATGCCTGC
    TTTTCTAGGG ATAGACAAAG AATAGCATCA TGTGGAGCTG ATAAGACACT TCAGGTGTTT
    AAAGCAGAAA GCGGAGAGAG ATTGCTGGAG ATAAGTGCTC ATGATGATGA AATACTTTGC
    TGCACTTTTT CTGCAGATGA TAAATATGTA GCAACTTGTT CAGCTGATAA AAAAGTGAAG
    ATCTGGAATT CAAGAACGGG CAAGTGTTTG TACGTATATG AAGAACACAC AGAGCAGGTC
    AATTGCTGCC AGTTCAATAA CAGAAGGGGC CAGTATCTTT TAGCCACCTG CTCAGATGAC
    ACTTATATCA AACTTTGGGA TTTGAAGGAT AAGTATTGTA GGAATACGAT GTTTGGTCAT
    GTAAATGCTG TCAGTCACTG TAGATTTTCA CCAAATGATG ACTATATTGC CAGCTGCTCA
    ACAGATGGAA CAGTGAAGCT TTGGAATGTG CATTCAGCAA ATGAACTGAG AACTATTGAG
    ATAAAAGATT TCTTCAGGAA TGCAGATGAG CAGCAAGATG ACGTGGAGGT TTTAGTGAAA
    TGTTGCTCTT GGTCCAGAAG TAGTGATACA ATTTTGGTCG TAGCCAAAAA TAAACTTTTG
    CTTTTTGATG TTACAACAAG TAATCTGTTA ACACAAGTCA TCATAAGTCA TCACAGTACA
    ATCCAATATT GCGACTTCTG CGCTGGGGAT GAGTTAGTAG CAGTTGCTTT GTCTCATTGT
    TCTATTGAGT TATGGAATAT TAAATCCTTA TCAAAAGTAG CAGATTGCAG AGGACACACG
    AGCTGGGTTC ACTGTGTGAC GTTTTCCTCA GATGGATCTT TATTTCTGAC ATCTTCTGAG
    GATCAGACAA TACGGATTTG GGAAACAAAC AAGGTGTGCA AGTCTTCTGA TGCCGTGCTA
    AAAAGTGAAC TGGATGTTGT GTTTCACAAT GGTGAAGTCA TGATTTTAGC AATTTACAAT
    CAGAAGCATA TACAACTCAT TAATGGAAAT ACAGACAGCA TTATTATGCA GACAGAACCT
    CAGGAATCCC TTATTTGCTG CTGTTGCTTA AGTGAAGATC TTAAATTTGC AGCATTTGGG
    CAGGAGAATG GAGCAATAAA GGTACTACGG TTGTTGGATG GGAGAGTTCT GACATCCCGG
    GAGGCCTACA GGACATCTGT GCAACATTGT CGATTTACCA CTGATTGTCA GACTCTGATT
    TCAAGTGCCC ATGATGCAGT CATACAGGTC TGGAATTGGC AGTTGAACGA GTGTGTGTTT
    CTAAAAGGGC ATAAGGAAGC AATCAAAGAC TTTGCATTTC TGGAGAATTC AAAACTTCTT
    TCCTGGTCAT TTGATGGAAC AGTTAAGGTT TGGAATATCA CCACTGGAAA AACAGAAAAA
    GATTTTGCTT GCCACAGAGG TGCTGTTCTT TCTTGTGCTG TCTCACCTGA TGGTAGCAGG
    TTTTCATCTA CTTCTGCTGA CAAAACTGCA AAGATATGGA GCTTTGAAAG TTCATCTGTT
    CTTCATGAAC TGAAAGGTCA TGAAGCCTGT GTGCGGTGCT CTGCTTTCTC TCCTAGTAAC
    AAATTACTGG CCACTGGAGA TGACAAAGGA GATATAAGGA TTTGGGACAT TTTAACAGGT
    GAATTACTTC ACTTCTGCTC CTCAGTTACT GTGGATGAAG AAGAACCAAC ACATGGTGGC
    TGGGTAACAG ACCTCTCGTT TTCTCCTGAC AGTAAAATGC TTGTGTCATC TGGAGGCTAT
    CTTAAGTGGT GGGATGTAAC TACTGGAGAA TCCTTGCAAA CCTTCTACAC GAACGGAACA
    AACCTCAAGT CAATACACGT GTCCCCTAAT TTTGACGTGT ACGTGACTGT TGATAATCTT
    GGTATTCTTT ATGTATTACG GAAGTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.