HECW2 cDNA ORF clone, Bos taurus(cattle)

The following HECW2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa227759 XM_024981179.1
Latest version!
Bos taurus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OBa16609 XM_010802220.2
Latest version!
Bos taurus E3 ubiquitin-protein ligase HECW2 (LOC531691), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa16609 XM_015462350.1
Latest version!
Bos taurus E3 ubiquitin-protein ligase HECW2 (LOC531691), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa227758 XM_024981178.1
Latest version!
Bos taurus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa227759
    Clone ID Related Accession (Same CDS sequence) XM_024981179.1
    Accession Version XM_024981179.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECW2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGAGCCCG AGATAAAAAT CTGTTTCAAA TACTACCACG GCATCAGTGG AGCCCTGCGA 
    GCCACGACCC CTTGCATCAC GGTGAAGAAC CCAGCTGTGA TGATGGGGGC AGAAGGCATG
    GAAGGAGGGG CTTCAGGAAA TCAGCATTCT CGGAAGCTTG TTAGCTTTAC TTTGTCAGAC
    CTTAGGGCAG TTGGACTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG
    TCCATTCAGC CAGGGAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAGGAGAGG
    AGGTCTACTA TCATCAGTAA CACCACTAAT CCAATTTGGC ATCGAGAGAA ATACTCCTTT
    TTTGCACTCC TTACTGATGT CTTAGAGATT GAAATTAAAG ACAAGTTTGC AAAGAGCCGG
    CCTATCATTA AGCGTTTCCT GGGGAAGCTC ACCATTCCAG TCCAGAGGCT ACTGGAGCGG
    CAAGCCATTG GTGACCAGAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAT
    GTGAGTGGGT ACCTCCAGTT CAGAGTGGAG GTCACATCTT CTGTGCATGA AGATGCCTCT
    CCAGAAGCTG TTGGCACAAT ACTTGGAGTC AGTTCTGTGA ACGGAGACCT AGGAAGCCCT
    TCTGATGAGG AGGACATGCC AGGGGGCCAT CACGACAGCC CAGTTTGTTC CAATGGACCA
    GTTTCTGAGG AAAGTGCTGA TGGGACACCC AAGCATTCTC TCAGGACTAG TTCTACTCTG
    GAAATAGACA CAGAAGAATT AACTTCTACT TCTTCAAGGA CCTCACCTCC CAGAGGACGT
    CAGGATTCAC TCAATGATTA TTTAGATGCT ATTGAACACA ATGGCCATTC TAGGTCAGGT
    GCAGCTACTT GCTCGGAGAG GTCCGTGGGA GCCTCTCCAA AGCTAAGGAG TAGTTTTCCC
    ACTGACACAA GACTCAATGC AATGCTTCAT ATTGACTCAG ATGAGGAAGA CCACGAGTTC
    CAGCAAGACC TGGGCTATCC ATCCTCCTTG GAGGAGGAAG GAGGGTTGAT CATGTTTAGC
    AGGGCATCAA GAACTGATGA TGGGAGCCTG GCATCTCAGA CAAAGGCAGA AGACAACCCA
    GTGGAGACTG AGGAAGCCTC CATTAATGAA GCTGCTTCCT TTGAGGATAA GCCAGGAAAT
    CTTCCAGAGC TTGCAGAGAC CTCTTTACCC TCAGGCCCGG TCCCAGATGA AAGCGAGAAT
    GGCCCAGAAT CTCAACCAAG TGTGGACCAG GGCAGCACTG AATTGTGTGG CTCTCAGGAG
    GTGGATCAGC CCACAAGTGG AGCTGACACG GGCACTTCTG ACACGTCCGG GGGGAGCCGA
    AGAGCTGTCA GTGAGACAGA GTCCCTTGAT CAGGGGTCTG AGCCTTCCCA GGTGTCCTCA
    GAAACAGAGC CCAGTGACCC TGCCAGGACA GAGAGTGTGA GCGAGGCCAG CACCAGGCCC
    GAGGGAGAGA GTGACCTGGA AGGGGCTGAC AGCTCCTGCA ATGAGAGTGT TACCACGCAG
    CTCTCCTCGG TGGAGACACG GTGCTCATCT CTTGAGAGCG CACGGTTTCC TGAAACTCCA
    GCCTTTTCTT CTCAGGAGGA GGAAGATGGA GCCTGTGCAG CAGAGCCCGC CAGCAGTGGC
    CCTGCTGAAG GGTCCCAGGA TTCCATATGC ACTGCTGGTT CCTTACCTGT GGTGCAGGTG
    CCCAGTGGAG AGGAGGAAGG GCCAGGGTCA GAATCAACCA CTGTACCTGA CCAGGAGGAG
    GTGGGGGAGG TCTGGCAGCG GAGGGGGAGC CTGGAGGGAG CTGCTGCTGC TGCTGAGAGC
    CAGCCCCAAG AAGAGGGTGC TGCTCGGGAT ACCCCAGGGG CTTGTGAAGG GGCCACCGCC
    CAGGAGGAAG GCGCGATTGG AGGTTGTCAA TCCAACGGCC ACCAGCCACT GCGATCGCTG
    CCTTCAGTTC GCCAGGATGT TAGCCGGTAC CAGAGGGTGG ACGAGGCTCT CCCACCAAAC
    TGGGAGGCGC GCATTGACAG CCACGGCAGG ATCTTCTACG TGGATCATGT AAACAGAACC
    ACGACGTGGC AGCGGCCCAC AGCTCCCCCT GCCCCGCAGG TGCTGCAGAG GTCCAACTCC
    ATACAGCAGA TGGAGCAGCT GAACCGGCGA TACCAGAGTA TCCGCAGAAC CATGACCAAC
    GAGAGGCCTG AGGAAAATAG CAATGCTATT GATGGGGCCG GGGAGGAAGC CGACTTTCAC
    CAAGCCAGTG CAGATTTCCG ACGGGAGAGC GTCCTGCCCC ACTCTACCTC CAGATCCAGG
    CTCACGTTGC TGTTACAGTC TCCCCCCGTG AAGTTCCTCA TCAGCCCAGA GTTCTTCACC
    GTGCTGCATT CCAACCCTAG TGCCTACCGC ATGTTTACAA ACAACACATG TTTGAAGCAC
    ATGATCACCA AAGTCCGGCG GGACACCCAC CACTTTGAAC GCTACCAGCA TAACCGGGAC
    CTCGTGGGAT TCCTCAACAT GTTTGCAAAC AAACAACTGG AGCTGCCAAG GGGCTGGGAA
    ATGAAACACG ATCATCAGGG CAAGGCATTT TTTGTTGACC ACAACTCCCG AACCACCACT
    TTCATCGATC CCCGACTCCC GCTTCAGAGC AGCAGACCCA CGAGCGCACT GGTGCATCGA
    CAACACCTGA CCCGGCAGCG CAGCCACAGC GCAGGCGAGG TAGGAGAAGA TTCTCGACAT
    GCAGGACCAC CAGTTCTTCC CAGGCCGTCG AGTACGTTCA ATACAGTCAG CAGGCCGCAG
    TACCAGGACA TGGTTCCAGT GGCTTACAAT GACAAGATTG TTGCATTTCT GCGCCAGCCC
    AACATCTTTG AAATTCTGCA GGAACGTCAA CCTGATCTTA CCAGGAATCA CTCACTCAGG
    GAGAAGATCC AATTTATCCG AACTGAAGGG ACTCCAGGAT TGGTGCGTCT TTCAAGTGAT
    GCAGACCTTG TCATGCTGCT GAGTTTATTT GAAGAAGAGA TAATGTCATA TGTGCCTCCA
    CATGCCTTAC TCCATCCCAG CTATTGTCAG TCCCCACGTG GCTCCCCTGT GTCCTCTCCC
    CAGAACTCAC CAGGTACTCA GCGTGCCAAT GCCCGCGCTC CAGCCCCTTA CAAGCGAGAC
    TTTGAAGCCA AACTAAGGAA CTTTTACAGG AAGTTAGAGA CTAAAGGATA TGGACAAGGC
    CCAGGGAAAT TAAAGTTAAT TATCAGAAGA GATCACTTGC TTGAAGATGC TTTTAATCAA
    ATTATGGGCT ACTCCAGAAA AGACCTGCAG AGAAATAAGC TGTACGTCAC ATTCGTCGGA
    GAGGAAGGGC TGGATTACAG TGGACCTTCC AGAGAGTTTT TCTTCCTGGT ATCCAGAGAA
    CTCTTTAACC CATATTATGG CTTATTTGAA TATTCAGCCA ATGACACATA CACAGTACAA
    ATAAGTCCCA TGTCTGCCTT TGTAGACAAT CACCATGAAT GGTTCCGGTT CAGTGGTAGG
    ATCCTTGGCC TTGCACTAAT TCACCAGTAT TTGTTGGATG CCTTCTTCAC ACGGCCCTTT
    TATAAGGCTC TTCTCAGAAT TCTCTGTGAC CTGAGTGATC TAGAATACCT TGATGAAGAG
    TTCCATCAGA GCCTGCAGTG GATGAAAGAC AATGACATCC ATGACATCCT GGACCTCACA
    TTCACTGTGA ACGAAGAAGT TTTTGGGCAG ATTACTGAAC GAGAATTAAA ACCAGGGGGT
    GCCAACATCC CAGTCACAGA GAAGAACAAG AAGGAGTACA TTGAGAGGAT GGTGAAATGG
    AGGATTGAGA GGGGTGTCGT GCAGCAGACA GAGAGCTTAG TGCGTGGCTT CTATGAGGTG
    GTGGATGCCA GGCTGGTATC TGTTTTTGAT GCAAGAGAAC TAGAACTGGT CATCGCAGGC
    ACAGCTGAAA TAGACCTAAG TGATTGGAGA AACAACACAG AATACAGAGG AGGATATCAT
    GACAATCACA TTGTAATTCG GTGGTTCTGG GCTGCAGTGG AAAGATTCAA CAATGAACAA
    CGACTGAGGT TGTTACAGTT TGTTACAGGC ACATCCAGCA TTCCCTATGA AGGATTTGCT
    TCTCTCCGAG GCAGTAATGG CCCAAGAAGA TTCTGTGTGG AGAAATGGGG GAAAATCACT
    GCTCTTCCCA GAGCTCACAC TTGTTTTAAC CGTCTGGATC TCCCTCCATA CCCATCCTTC
    TCCATGCTTT ATGAAAAACT CTTGACAGCA GTTGAAGAGA CCAGTACCTT TGGACTTGAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836947.1
    CDS487..4809
    Translation

    Target ORF information:

    RefSeq Version XM_024981179.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981179.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGAGCCCG AGATAAAAAT CTGTTTCAAA TACTACCACG GCATCAGTGG AGCCCTGCGA 
    GCCACGACCC CTTGCATCAC GGTGAAGAAC CCAGCTGTGA TGATGGGGGC AGAAGGCATG
    GAAGGAGGGG CTTCAGGAAA TCAGCATTCT CGGAAGCTTG TTAGCTTTAC TTTGTCAGAC
    CTTAGGGCAG TTGGACTAAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG
    TCCATTCAGC CAGGGAAGAA GAGCAGTTTC CCCACCTGTG CCCACCATGG GCAGGAGAGG
    AGGTCTACTA TCATCAGTAA CACCACTAAT CCAATTTGGC ATCGAGAGAA ATACTCCTTT
    TTTGCACTCC TTACTGATGT CTTAGAGATT GAAATTAAAG ACAAGTTTGC AAAGAGCCGG
    CCTATCATTA AGCGTTTCCT GGGGAAGCTC ACCATTCCAG TCCAGAGGCT ACTGGAGCGG
    CAAGCCATTG GTGACCAGAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC AGCTGACCAT
    GTGAGTGGGT ACCTCCAGTT CAGAGTGGAG GTCACATCTT CTGTGCATGA AGATGCCTCT
    CCAGAAGCTG TTGGCACAAT ACTTGGAGTC AGTTCTGTGA ACGGAGACCT AGGAAGCCCT
    TCTGATGAGG AGGACATGCC AGGGGGCCAT CACGACAGCC CAGTTTGTTC CAATGGACCA
    GTTTCTGAGG AAAGTGCTGA TGGGACACCC AAGCATTCTC TCAGGACTAG TTCTACTCTG
    GAAATAGACA CAGAAGAATT AACTTCTACT TCTTCAAGGA CCTCACCTCC CAGAGGACGT
    CAGGATTCAC TCAATGATTA TTTAGATGCT ATTGAACACA ATGGCCATTC TAGGTCAGGT
    GCAGCTACTT GCTCGGAGAG GTCCGTGGGA GCCTCTCCAA AGCTAAGGAG TAGTTTTCCC
    ACTGACACAA GACTCAATGC AATGCTTCAT ATTGACTCAG ATGAGGAAGA CCACGAGTTC
    CAGCAAGACC TGGGCTATCC ATCCTCCTTG GAGGAGGAAG GAGGGTTGAT CATGTTTAGC
    AGGGCATCAA GAACTGATGA TGGGAGCCTG GCATCTCAGA CAAAGGCAGA AGACAACCCA
    GTGGAGACTG AGGAAGCCTC CATTAATGAA GCTGCTTCCT TTGAGGATAA GCCAGGAAAT
    CTTCCAGAGC TTGCAGAGAC CTCTTTACCC TCAGGCCCGG TCCCAGATGA AAGCGAGAAT
    GGCCCAGAAT CTCAACCAAG TGTGGACCAG GGCAGCACTG AATTGTGTGG CTCTCAGGAG
    GTGGATCAGC CCACAAGTGG AGCTGACACG GGCACTTCTG ACACGTCCGG GGGGAGCCGA
    AGAGCTGTCA GTGAGACAGA GTCCCTTGAT CAGGGGTCTG AGCCTTCCCA GGTGTCCTCA
    GAAACAGAGC CCAGTGACCC TGCCAGGACA GAGAGTGTGA GCGAGGCCAG CACCAGGCCC
    GAGGGAGAGA GTGACCTGGA AGGGGCTGAC AGCTCCTGCA ATGAGAGTGT TACCACGCAG
    CTCTCCTCGG TGGAGACACG GTGCTCATCT CTTGAGAGCG CACGGTTTCC TGAAACTCCA
    GCCTTTTCTT CTCAGGAGGA GGAAGATGGA GCCTGTGCAG CAGAGCCCGC CAGCAGTGGC
    CCTGCTGAAG GGTCCCAGGA TTCCATATGC ACTGCTGGTT CCTTACCTGT GGTGCAGGTG
    CCCAGTGGAG AGGAGGAAGG GCCAGGGTCA GAATCAACCA CTGTACCTGA CCAGGAGGAG
    GTGGGGGAGG TCTGGCAGCG GAGGGGGAGC CTGGAGGGAG CTGCTGCTGC TGCTGAGAGC
    CAGCCCCAAG AAGAGGGTGC TGCTCGGGAT ACCCCAGGGG CTTGTGAAGG GGCCACCGCC
    CAGGAGGAAG GCGCGATTGG AGGTTGTCAA TCCAACGGCC ACCAGCCACT GCGATCGCTG
    CCTTCAGTTC GCCAGGATGT TAGCCGGTAC CAGAGGGTGG ACGAGGCTCT CCCACCAAAC
    TGGGAGGCGC GCATTGACAG CCACGGCAGG ATCTTCTACG TGGATCATGT AAACAGAACC
    ACGACGTGGC AGCGGCCCAC AGCTCCCCCT GCCCCGCAGG TGCTGCAGAG GTCCAACTCC
    ATACAGCAGA TGGAGCAGCT GAACCGGCGA TACCAGAGTA TCCGCAGAAC CATGACCAAC
    GAGAGGCCTG AGGAAAATAG CAATGCTATT GATGGGGCCG GGGAGGAAGC CGACTTTCAC
    CAAGCCAGTG CAGATTTCCG ACGGGAGAGC GTCCTGCCCC ACTCTACCTC CAGATCCAGG
    CTCACGTTGC TGTTACAGTC TCCCCCCGTG AAGTTCCTCA TCAGCCCAGA GTTCTTCACC
    GTGCTGCATT CCAACCCTAG TGCCTACCGC ATGTTTACAA ACAACACATG TTTGAAGCAC
    ATGATCACCA AAGTCCGGCG GGACACCCAC CACTTTGAAC GCTACCAGCA TAACCGGGAC
    CTCGTGGGAT TCCTCAACAT GTTTGCAAAC AAACAACTGG AGCTGCCAAG GGGCTGGGAA
    ATGAAACACG ATCATCAGGG CAAGGCATTT TTTGTTGACC ACAACTCCCG AACCACCACT
    TTCATCGATC CCCGACTCCC GCTTCAGAGC AGCAGACCCA CGAGCGCACT GGTGCATCGA
    CAACACCTGA CCCGGCAGCG CAGCCACAGC GCAGGCGAGG TAGGAGAAGA TTCTCGACAT
    GCAGGACCAC CAGTTCTTCC CAGGCCGTCG AGTACGTTCA ATACAGTCAG CAGGCCGCAG
    TACCAGGACA TGGTTCCAGT GGCTTACAAT GACAAGATTG TTGCATTTCT GCGCCAGCCC
    AACATCTTTG AAATTCTGCA GGAACGTCAA CCTGATCTTA CCAGGAATCA CTCACTCAGG
    GAGAAGATCC AATTTATCCG AACTGAAGGG ACTCCAGGAT TGGTGCGTCT TTCAAGTGAT
    GCAGACCTTG TCATGCTGCT GAGTTTATTT GAAGAAGAGA TAATGTCATA TGTGCCTCCA
    CATGCCTTAC TCCATCCCAG CTATTGTCAG TCCCCACGTG GCTCCCCTGT GTCCTCTCCC
    CAGAACTCAC CAGGTACTCA GCGTGCCAAT GCCCGCGCTC CAGCCCCTTA CAAGCGAGAC
    TTTGAAGCCA AACTAAGGAA CTTTTACAGG AAGTTAGAGA CTAAAGGATA TGGACAAGGC
    CCAGGGAAAT TAAAGTTAAT TATCAGAAGA GATCACTTGC TTGAAGATGC TTTTAATCAA
    ATTATGGGCT ACTCCAGAAA AGACCTGCAG AGAAATAAGC TGTACGTCAC ATTCGTCGGA
    GAGGAAGGGC TGGATTACAG TGGACCTTCC AGAGAGTTTT TCTTCCTGGT ATCCAGAGAA
    CTCTTTAACC CATATTATGG CTTATTTGAA TATTCAGCCA ATGACACATA CACAGTACAA
    ATAAGTCCCA TGTCTGCCTT TGTAGACAAT CACCATGAAT GGTTCCGGTT CAGTGGTAGG
    ATCCTTGGCC TTGCACTAAT TCACCAGTAT TTGTTGGATG CCTTCTTCAC ACGGCCCTTT
    TATAAGGCTC TTCTCAGAAT TCTCTGTGAC CTGAGTGATC TAGAATACCT TGATGAAGAG
    TTCCATCAGA GCCTGCAGTG GATGAAAGAC AATGACATCC ATGACATCCT GGACCTCACA
    TTCACTGTGA ACGAAGAAGT TTTTGGGCAG ATTACTGAAC GAGAATTAAA ACCAGGGGGT
    GCCAACATCC CAGTCACAGA GAAGAACAAG AAGGAGTACA TTGAGAGGAT GGTGAAATGG
    AGGATTGAGA GGGGTGTCGT GCAGCAGACA GAGAGCTTAG TGCGTGGCTT CTATGAGGTG
    GTGGATGCCA GGCTGGTATC TGTTTTTGAT GCAAGAGAAC TAGAACTGGT CATCGCAGGC
    ACAGCTGAAA TAGACCTAAG TGATTGGAGA AACAACACAG AATACAGAGG AGGATATCAT
    GACAATCACA TTGTAATTCG GTGGTTCTGG GCTGCAGTGG AAAGATTCAA CAATGAACAA
    CGACTGAGGT TGTTACAGTT TGTTACAGGC ACATCCAGCA TTCCCTATGA AGGATTTGCT
    TCTCTCCGAG GCAGTAATGG CCCAAGAAGA TTCTGTGTGG AGAAATGGGG GAAAATCACT
    GCTCTTCCCA GAGCTCACAC TTGTTTTAAC CGTCTGGATC TCCCTCCATA CCCATCCTTC
    TCCATGCTTT ATGAAAAACT CTTGACAGCA GTTGAAGAGA CCAGTACCTT TGGACTTGAG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16609
    Clone ID Related Accession (Same CDS sequence) XM_010802220.2 , XM_015462350.1
    Accession Version XM_010802220.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECW2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103845.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010802220.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 18% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTAGCT CAGCCCGGGA ACACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
    TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACCGAGAAT
    ATGACCCTAC AGCGGGCCAA CAGCGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC
    TTGACGGCCA GCATGTATGA GTACACACTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG
    GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TGTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG
    AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT
    TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGACT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT
    AATCCAATTT GGCATCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG
    ATTGAAATTA AAGACAAGTT TGCAAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG
    CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTCAAGA CAGTAGGTGC
    CCCAACCCTT TGGCAGTGCG AGTTAGAGAC TGCAAGGGGG CACAAGGAAC AAGCGATGTG
    GTGCCGGCGG CCCAAGTGGG CTCAGCTGCC CTTTCATCTG GTGGAACAGT GCCAGCCGGG
    AGACGACAGC TAAGCACTTT CCAGACTGTA TCTGCTCGGT CGAGTGGCAG CTGCCAGCTC
    CCTTCTTCTG GGGTCCTGGG CATCTATCCT TTAAGTCCCG TGGGTCCTAT CACCCTTGAA
    CCCTTTGGTC AAGGTATCCC TGAGACACAG GCCACATTGT CTTCTACATC CTACCTCCAA
    TCGCGCCTTC CTCCTGGGCG GTGGCCCCTT CACAAGCCCC TGGGGTATCC CGAGCAGCAC
    CCTCTTCTTG GGGCTGGCTC TCAGCAGCAG CAGCAGCTCC CTCCAGGCTC CCCCTCCGCT
    GCCAGACCTC CCCCACCTCC TCCTGGTCAG GTACAGTGGT TGATTCTGAC CCTGGCCCTT
    CCTCCTCTCC ACTGGGCACC TGCACCACAG GTAAGGAACC AGCAGTGCAT ATGGAATCCT
    GGGACCCTTC AGCAGGGCCA CTGCTGGCGG GCTCTGCTGC ACAGGCTCCA TCTTCCTCCT
    CCTGAGAAGA AAAGGCTGGA GTTTCAGGAA ACCGTGCGCT CTCAAGAGAT GAGCACCGTG
    TCTCCACCGA GGAGAGCTGC GTGCCTCTCC ATGCATTACT GCTGTTTAGA CTGGGAGGCG
    CGCATTGACA GCCACGGCAG GATCTTCTAC GTGGATCATG TAAACAGAAC CACGACGTGG
    CAGCGGCCCA CAGCTCCCCC TGCCCCGCAG GTGCTGCAGA GGTCCAACTC CATACAGCAG
    ATGGAGCAGC TGAACCGGCG ATACCAGAGT ATCCGCAGAA CCATGACCAA CGAGAGGCCT
    GAGGAAAATA GCAATGCTAT TGATGGGGCC GGGGAGGAAG CCGACTTTCA CCAAGCCAGT
    GCAGATTTCC GACGGGAGAG CGTCCTGCCC CACTCTACCT CCAGATCCAG GCTCACGTTG
    CTGTTACAGT CTCCCCCCGT GAAGTTCCTC ATCAGCCCAG AGTTCTTCAC CGTGCTGCAT
    TCCAACCCTA GTGCCTACCG CATGTTTACA AACAACACAT GTTTGAAGCA CATGATCACC
    AAAGTCCGGC GGGACACCCA CCACTTTGAA CGCTACCAGC ATAACCGGGA CCTCGTGGGA
    TTCCTCAACA TGTTTGCAAA CAAACAACTG GAGCTGCCAA GGGGCTGGGA AATGAAACAC
    GATCATCAGG GCAAGGCATT TTTTGTTGAC CACAACTCCC GAACCACCAC TTTCATCGAT
    CCCCGACTCC CGCTTCAGAG CAGCAGACCC ACGAGCGCAC TGGTGCATCG ACAACACCTG
    ACCCGGCAGC GCAGCCACAG CGCAGGCGAG GTAGGAGAAG ATTCTCGACA TGCAGGACCA
    CCAGTTCTTC CCAGGCCGTC GAGTACGTTC AATACAGTCA GCAGGCCGCA GTACCAGGAC
    ATGGTTCCAG TGGCTTACAA TGACAAGATT GTTGCATTTC TGCGCCAGCC CAACATCTTT
    GAAATTCTGC AGGAACGTCA ACCTGATCTT ACCAGGAATC ACTCACTCAG GGAGAAGATC
    CAATTTATCC GAACTGAAGG GACTCCAGGA TTGGTGCGTC TTTCAAGTGA TGCAGACCTT
    GTCATGCTGC TGAGTTTATT TGAAGAAGAG ATAATGTCAT ATGTGCCTCC ACATGCCTTA
    CTCCATCCCA GCTACTGTCA GTCCCCACGT GGCTCCCCTG TGTCCTCTCC CCAGAACTCA
    CCAGGTACTC AGCGTGCCAA TGCCCGCGCT CCAGCCCCTT ACAAGCGAGA CTTTGAAGCC
    AAACTAAGGA ACTTTTACAG GAAGTTAGAG ACTAAAGGAT ATGGACAAGG CCCAGGGAAA
    TTAAAGTTAA TTATCAGAAG AGATCACTTG CTTGAAGATG CTTTTAATCA AATTATGGGC
    TACTCCAGAA AAGACCTGCA GAGAAATAAG CTGTACGTCA CATTCGTCGG AGAGGAAGGG
    CTGGATTACA GTGGACCTTC CAGAGAGTTT TTCTTCCTGG TATCCAGAGA ACTCTTTAAC
    CCATATTATG GCTTATTTGA ATATTCAGCC AATGACACAT ACACAGTACA AATAAGTCCC
    ATGTCTGCCT TTGTAGACAA TCACCATGAA TGGTTCCGGT TCAGTGGTAG GATCCTTGGC
    CTTGCACTAA TTCACCAGTA TTTGTTGGAT GCCTTCTTCA CACGGCCCTT TTATAAGGCT
    CTTCTCAGAA TTCTCTGTGA CCTGAGTGAT CTAGAATACC TTGATGAAGA GTTCCATCAG
    AGCCTGCAGT GGATGAAAGA CAATGACATC CATGACATCC TGGACCTCAC ATTCACTGTG
    AACGAAGAAG TTTTTGGGCA GATTACTGAA CGAGAATTAA AACCAGGGGG TGCCAACATC
    CCAGTCACAG AGAAGAACAA GAAGGAGTAC ATTGAGAGGA TGGTGAAATG GAGGATTGAG
    AGGGGTGTCG TGCAGCAGAC AGAGAGCTTA GTGCGTGGCT TCTATGAGGT GGTGGATGCC
    AGGCTGGTAT CTGTTTTTGA TGCAAGAGAA CTAGAACTGG TCATCGCAGG CACAGCTGAA
    ATAGACCTAA GTGATTGGAG AAACAACACA GAATACAGAG GAGGATATCA TGACAATCAC
    ATTGTAATTC GGTGGTTCTG GGCTGCAGTG GAAAGATTCA ACAATGAACA ACGACTGAGG
    TTGTTACAGT TTGTTACAGG CACATCCAGC ATTCCCTATG AAGGATTTGC TTCTCTCCGA
    GGCAGTAATG GCCCAAGAAG ATTCTGTGTG GAGAAATGGG GGAAAATCAC TGCTCTTCCC
    AGAGCTCACA CTTGTTTTAA CCGTCTGGAT CTCCCTCCAT ACCCATCCTT CTCCATGCTT
    TATGAAAAAC TCTTGACAGC AGTTGAAGAG ACCAGTACCT TTGGACTTGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010800522.1
    CDS125..4018
    Translation

    Target ORF information:

    RefSeq Version XM_010802220.2
    Organism Bos taurus(cattle)
    Definition Bos taurus E3 ubiquitin-protein ligase HECW2 (LOC531691), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010802220.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTAGCT CAGCCCGGGA ACACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
    TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACCGAGAAT
    ATGACCCTAC AGCGGGCCAA CAGCGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC
    TTGACGGCCA GCATGTATGA GTACACACTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG
    GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TGTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG
    AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT
    TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGACT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT
    AATCCAATTT GGCATCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG
    ATTGAAATTA AAGACAAGTT TGCAAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG
    CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTCAAGA CAGTAGGTGC
    CCCAACCCTT TGGCAGTGCG AGTTAGAGAC TGCAAGGGGG CACAAGGAAC AAGCGATGTG
    GTGCCGGCGG CCCAAGTGGG CTCAGCTGCC CTTTCATCTG GTGGAACAGT GCCAGCCGGG
    AGACGACAGC TAAGCACTTT CCAGACTGTA TCTGCTCGGT CGAGTGGCAG CTGCCAGCTC
    CCTTCTTCTG GGGTCCTGGG CATCTATCCT TTAAGTCCCG TGGGTCCTAT CACCCTTGAA
    CCCTTTGGTC AAGGTATCCC TGAGACACAG GCCACATTGT CTTCTACATC CTACCTCCAA
    TCGCGCCTTC CTCCTGGGCG GTGGCCCCTT CACAAGCCCC TGGGGTATCC CGAGCAGCAC
    CCTCTTCTTG GGGCTGGCTC TCAGCAGCAG CAGCAGCTCC CTCCAGGCTC CCCCTCCGCT
    GCCAGACCTC CCCCACCTCC TCCTGGTCAG GTACAGTGGT TGATTCTGAC CCTGGCCCTT
    CCTCCTCTCC ACTGGGCACC TGCACCACAG GTAAGGAACC AGCAGTGCAT ATGGAATCCT
    GGGACCCTTC AGCAGGGCCA CTGCTGGCGG GCTCTGCTGC ACAGGCTCCA TCTTCCTCCT
    CCTGAGAAGA AAAGGCTGGA GTTTCAGGAA ACCGTGCGCT CTCAAGAGAT GAGCACCGTG
    TCTCCACCGA GGAGAGCTGC GTGCCTCTCC ATGCATTACT GCTGTTTAGA CTGGGAGGCG
    CGCATTGACA GCCACGGCAG GATCTTCTAC GTGGATCATG TAAACAGAAC CACGACGTGG
    CAGCGGCCCA CAGCTCCCCC TGCCCCGCAG GTGCTGCAGA GGTCCAACTC CATACAGCAG
    ATGGAGCAGC TGAACCGGCG ATACCAGAGT ATCCGCAGAA CCATGACCAA CGAGAGGCCT
    GAGGAAAATA GCAATGCTAT TGATGGGGCC GGGGAGGAAG CCGACTTTCA CCAAGCCAGT
    GCAGATTTCC GACGGGAGAG CGTCCTGCCC CACTCTACCT CCAGATCCAG GCTCACGTTG
    CTGTTACAGT CTCCCCCCGT GAAGTTCCTC ATCAGCCCAG AGTTCTTCAC CGTGCTGCAT
    TCCAACCCTA GTGCCTACCG CATGTTTACA AACAACACAT GTTTGAAGCA CATGATCACC
    AAAGTCCGGC GGGACACCCA CCACTTTGAA CGCTACCAGC ATAACCGGGA CCTCGTGGGA
    TTCCTCAACA TGTTTGCAAA CAAACAACTG GAGCTGCCAA GGGGCTGGGA AATGAAACAC
    GATCATCAGG GCAAGGCATT TTTTGTTGAC CACAACTCCC GAACCACCAC TTTCATCGAT
    CCCCGACTCC CGCTTCAGAG CAGCAGACCC ACGAGCGCAC TGGTGCATCG ACAACACCTG
    ACCCGGCAGC GCAGCCACAG CGCAGGCGAG GTAGGAGAAG ATTCTCGACA TGCAGGACCA
    CCAGTTCTTC CCAGGCCGTC GAGTACGTTC AATACAGTCA GCAGGCCGCA GTACCAGGAC
    ATGGTTCCAG TGGCTTACAA TGACAAGATT GTTGCATTTC TGCGCCAGCC CAACATCTTT
    GAAATTCTGC AGGAACGTCA ACCTGATCTT ACCAGGAATC ACTCACTCAG GGAGAAGATC
    CAATTTATCC GAACTGAAGG GACTCCAGGA TTGGTGCGTC TTTCAAGTGA TGCAGACCTT
    GTCATGCTGC TGAGTTTATT TGAAGAAGAG ATAATGTCAT ATGTGCCTCC ACATGCCTTA
    CTCCATCCCA GCTACTGTCA GTCCCCACGT GGCTCCCCTG TGTCCTCTCC CCAGAACTCA
    CCAGGTACTC AGCGTGCCAA TGCCCGCGCT CCAGCCCCTT ACAAGCGAGA CTTTGAAGCC
    AAACTAAGGA ACTTTTACAG GAAGTTAGAG ACTAAAGGAT ATGGACAAGG CCCAGGGAAA
    TTAAAGTTAA TTATCAGAAG AGATCACTTG CTTGAAGATG CTTTTAATCA AATTATGGGC
    TACTCCAGAA AAGACCTGCA GAGAAATAAG CTGTACGTCA CATTCGTCGG AGAGGAAGGG
    CTGGATTACA GTGGACCTTC CAGAGAGTTT TTCTTCCTGG TATCCAGAGA ACTCTTTAAC
    CCATATTATG GCTTATTTGA ATATTCAGCC AATGACACAT ACACAGTACA AATAAGTCCC
    ATGTCTGCCT TTGTAGACAA TCACCATGAA TGGTTCCGGT TCAGTGGTAG GATCCTTGGC
    CTTGCACTAA TTCACCAGTA TTTGTTGGAT GCCTTCTTCA CACGGCCCTT TTATAAGGCT
    CTTCTCAGAA TTCTCTGTGA CCTGAGTGAT CTAGAATACC TTGATGAAGA GTTCCATCAG
    AGCCTGCAGT GGATGAAAGA CAATGACATC CATGACATCC TGGACCTCAC ATTCACTGTG
    AACGAAGAAG TTTTTGGGCA GATTACTGAA CGAGAATTAA AACCAGGGGG TGCCAACATC
    CCAGTCACAG AGAAGAACAA GAAGGAGTAC ATTGAGAGGA TGGTGAAATG GAGGATTGAG
    AGGGGTGTCG TGCAGCAGAC AGAGAGCTTA GTGCGTGGCT TCTATGAGGT GGTGGATGCC
    AGGCTGGTAT CTGTTTTTGA TGCAAGAGAA CTAGAACTGG TCATCGCAGG CACAGCTGAA
    ATAGACCTAA GTGATTGGAG AAACAACACA GAATACAGAG GAGGATATCA TGACAATCAC
    ATTGTAATTC GGTGGTTCTG GGCTGCAGTG GAAAGATTCA ACAATGAACA ACGACTGAGG
    TTGTTACAGT TTGTTACAGG CACATCCAGC ATTCCCTATG AAGGATTTGC TTCTCTCCGA
    GGCAGTAATG GCCCAAGAAG ATTCTGTGTG GAGAAATGGG GGAAAATCAC TGCTCTTCCC
    AGAGCTCACA CTTGTTTTAA CCGTCTGGAT CTCCCTCCAT ACCCATCCTT CTCCATGCTT
    TATGAAAAAC TCTTGACAGC AGTTGAAGAG ACCAGTACCT TTGGACTTGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa16609
    Clone ID Related Accession (Same CDS sequence) XM_010802220.2 , XM_015462350.1
    Accession Version XM_015462350.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECW2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644605.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 18% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTAGCT CAGCCCGGGA ACACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
    TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACCGAGAAT
    ATGACCCTAC AGCGGGCCAA CAGCGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC
    TTGACGGCCA GCATGTATGA GTACACACTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG
    GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TGTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG
    AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT
    TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGACT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT
    AATCCAATTT GGCATCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG
    ATTGAAATTA AAGACAAGTT TGCAAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG
    CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTCAAGA CAGTAGGTGC
    CCCAACCCTT TGGCAGTGCG AGTTAGAGAC TGCAAGGGGG CACAAGGAAC AAGCGATGTG
    GTGCCGGCGG CCCAAGTGGG CTCAGCTGCC CTTTCATCTG GTGGAACAGT GCCAGCCGGG
    AGACGACAGC TAAGCACTTT CCAGACTGTA TCTGCTCGGT CGAGTGGCAG CTGCCAGCTC
    CCTTCTTCTG GGGTCCTGGG CATCTATCCT TTAAGTCCCG TGGGTCCTAT CACCCTTGAA
    CCCTTTGGTC AAGGTATCCC TGAGACACAG GCCACATTGT CTTCTACATC CTACCTCCAA
    TCGCGCCTTC CTCCTGGGCG GTGGCCCCTT CACAAGCCCC TGGGGTATCC CGAGCAGCAC
    CCTCTTCTTG GGGCTGGCTC TCAGCAGCAG CAGCAGCTCC CTCCAGGCTC CCCCTCCGCT
    GCCAGACCTC CCCCACCTCC TCCTGGTCAG GTACAGTGGT TGATTCTGAC CCTGGCCCTT
    CCTCCTCTCC ACTGGGCACC TGCACCACAG GTAAGGAACC AGCAGTGCAT ATGGAATCCT
    GGGACCCTTC AGCAGGGCCA CTGCTGGCGG GCTCTGCTGC ACAGGCTCCA TCTTCCTCCT
    CCTGAGAAGA AAAGGCTGGA GTTTCAGGAA ACCGTGCGCT CTCAAGAGAT GAGCACCGTG
    TCTCCACCGA GGAGAGCTGC GTGCCTCTCC ATGCATTACT GCTGTTTAGA CTGGGAGGCG
    CGCATTGACA GCCACGGCAG GATCTTCTAC GTGGATCATG TAAACAGAAC CACGACGTGG
    CAGCGGCCCA CAGCTCCCCC TGCCCCGCAG GTGCTGCAGA GGTCCAACTC CATACAGCAG
    ATGGAGCAGC TGAACCGGCG ATACCAGAGT ATCCGCAGAA CCATGACCAA CGAGAGGCCT
    GAGGAAAATA GCAATGCTAT TGATGGGGCC GGGGAGGAAG CCGACTTTCA CCAAGCCAGT
    GCAGATTTCC GACGGGAGAG CGTCCTGCCC CACTCTACCT CCAGATCCAG GCTCACGTTG
    CTGTTACAGT CTCCCCCCGT GAAGTTCCTC ATCAGCCCAG AGTTCTTCAC CGTGCTGCAT
    TCCAACCCTA GTGCCTACCG CATGTTTACA AACAACACAT GTTTGAAGCA CATGATCACC
    AAAGTCCGGC GGGACACCCA CCACTTTGAA CGCTACCAGC ATAACCGGGA CCTCGTGGGA
    TTCCTCAACA TGTTTGCAAA CAAACAACTG GAGCTGCCAA GGGGCTGGGA AATGAAACAC
    GATCATCAGG GCAAGGCATT TTTTGTTGAC CACAACTCCC GAACCACCAC TTTCATCGAT
    CCCCGACTCC CGCTTCAGAG CAGCAGACCC ACGAGCGCAC TGGTGCATCG ACAACACCTG
    ACCCGGCAGC GCAGCCACAG CGCAGGCGAG GTAGGAGAAG ATTCTCGACA TGCAGGACCA
    CCAGTTCTTC CCAGGCCGTC GAGTACGTTC AATACAGTCA GCAGGCCGCA GTACCAGGAC
    ATGGTTCCAG TGGCTTACAA TGACAAGATT GTTGCATTTC TGCGCCAGCC CAACATCTTT
    GAAATTCTGC AGGAACGTCA ACCTGATCTT ACCAGGAATC ACTCACTCAG GGAGAAGATC
    CAATTTATCC GAACTGAAGG GACTCCAGGA TTGGTGCGTC TTTCAAGTGA TGCAGACCTT
    GTCATGCTGC TGAGTTTATT TGAAGAAGAG ATAATGTCAT ATGTGCCTCC ACATGCCTTA
    CTCCATCCCA GCTACTGTCA GTCCCCACGT GGCTCCCCTG TGTCCTCTCC CCAGAACTCA
    CCAGGTACTC AGCGTGCCAA TGCCCGCGCT CCAGCCCCTT ACAAGCGAGA CTTTGAAGCC
    AAACTAAGGA ACTTTTACAG GAAGTTAGAG ACTAAAGGAT ATGGACAAGG CCCAGGGAAA
    TTAAAGTTAA TTATCAGAAG AGATCACTTG CTTGAAGATG CTTTTAATCA AATTATGGGC
    TACTCCAGAA AAGACCTGCA GAGAAATAAG CTGTACGTCA CATTCGTCGG AGAGGAAGGG
    CTGGATTACA GTGGACCTTC CAGAGAGTTT TTCTTCCTGG TATCCAGAGA ACTCTTTAAC
    CCATATTATG GCTTATTTGA ATATTCAGCC AATGACACAT ACACAGTACA AATAAGTCCC
    ATGTCTGCCT TTGTAGACAA TCACCATGAA TGGTTCCGGT TCAGTGGTAG GATCCTTGGC
    CTTGCACTAA TTCACCAGTA TTTGTTGGAT GCCTTCTTCA CACGGCCCTT TTATAAGGCT
    CTTCTCAGAA TTCTCTGTGA CCTGAGTGAT CTAGAATACC TTGATGAAGA GTTCCATCAG
    AGCCTGCAGT GGATGAAAGA CAATGACATC CATGACATCC TGGACCTCAC ATTCACTGTG
    AACGAAGAAG TTTTTGGGCA GATTACTGAA CGAGAATTAA AACCAGGGGG TGCCAACATC
    CCAGTCACAG AGAAGAACAA GAAGGAGTAC ATTGAGAGGA TGGTGAAATG GAGGATTGAG
    AGGGGTGTCG TGCAGCAGAC AGAGAGCTTA GTGCGTGGCT TCTATGAGGT GGTGGATGCC
    AGGCTGGTAT CTGTTTTTGA TGCAAGAGAA CTAGAACTGG TCATCGCAGG CACAGCTGAA
    ATAGACCTAA GTGATTGGAG AAACAACACA GAATACAGAG GAGGATATCA TGACAATCAC
    ATTGTAATTC GGTGGTTCTG GGCTGCAGTG GAAAGATTCA ACAATGAACA ACGACTGAGG
    TTGTTACAGT TTGTTACAGG CACATCCAGC ATTCCCTATG AAGGATTTGC TTCTCTCCGA
    GGCAGTAATG GCCCAAGAAG ATTCTGTGTG GAGAAATGGG GGAAAATCAC TGCTCTTCCC
    AGAGCTCACA CTTGTTTTAA CCGTCTGGAT CTCCCTCCAT ACCCATCCTT CTCCATGCTT
    TATGAAAAAC TCTTGACAGC AGTTGAAGAG ACCAGTACCT TTGGACTTGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015317836.1
    CDS125..4018
    Translation

    Target ORF information:

    RefSeq Version XM_015462350.1
    Organism Bos taurus(cattle)
    Definition Bos taurus E3 ubiquitin-protein ligase HECW2 (LOC531691), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015462350.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCTAGCT CAGCCCGGGA ACACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
    TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACCGAGAAT
    ATGACCCTAC AGCGGGCCAA CAGCGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC
    TTGACGGCCA GCATGTATGA GTACACACTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG
    GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TGTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG
    AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT
    TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGACT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT
    AATCCAATTT GGCATCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG
    ATTGAAATTA AAGACAAGTT TGCAAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG
    CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTCAAGA CAGTAGGTGC
    CCCAACCCTT TGGCAGTGCG AGTTAGAGAC TGCAAGGGGG CACAAGGAAC AAGCGATGTG
    GTGCCGGCGG CCCAAGTGGG CTCAGCTGCC CTTTCATCTG GTGGAACAGT GCCAGCCGGG
    AGACGACAGC TAAGCACTTT CCAGACTGTA TCTGCTCGGT CGAGTGGCAG CTGCCAGCTC
    CCTTCTTCTG GGGTCCTGGG CATCTATCCT TTAAGTCCCG TGGGTCCTAT CACCCTTGAA
    CCCTTTGGTC AAGGTATCCC TGAGACACAG GCCACATTGT CTTCTACATC CTACCTCCAA
    TCGCGCCTTC CTCCTGGGCG GTGGCCCCTT CACAAGCCCC TGGGGTATCC CGAGCAGCAC
    CCTCTTCTTG GGGCTGGCTC TCAGCAGCAG CAGCAGCTCC CTCCAGGCTC CCCCTCCGCT
    GCCAGACCTC CCCCACCTCC TCCTGGTCAG GTACAGTGGT TGATTCTGAC CCTGGCCCTT
    CCTCCTCTCC ACTGGGCACC TGCACCACAG GTAAGGAACC AGCAGTGCAT ATGGAATCCT
    GGGACCCTTC AGCAGGGCCA CTGCTGGCGG GCTCTGCTGC ACAGGCTCCA TCTTCCTCCT
    CCTGAGAAGA AAAGGCTGGA GTTTCAGGAA ACCGTGCGCT CTCAAGAGAT GAGCACCGTG
    TCTCCACCGA GGAGAGCTGC GTGCCTCTCC ATGCATTACT GCTGTTTAGA CTGGGAGGCG
    CGCATTGACA GCCACGGCAG GATCTTCTAC GTGGATCATG TAAACAGAAC CACGACGTGG
    CAGCGGCCCA CAGCTCCCCC TGCCCCGCAG GTGCTGCAGA GGTCCAACTC CATACAGCAG
    ATGGAGCAGC TGAACCGGCG ATACCAGAGT ATCCGCAGAA CCATGACCAA CGAGAGGCCT
    GAGGAAAATA GCAATGCTAT TGATGGGGCC GGGGAGGAAG CCGACTTTCA CCAAGCCAGT
    GCAGATTTCC GACGGGAGAG CGTCCTGCCC CACTCTACCT CCAGATCCAG GCTCACGTTG
    CTGTTACAGT CTCCCCCCGT GAAGTTCCTC ATCAGCCCAG AGTTCTTCAC CGTGCTGCAT
    TCCAACCCTA GTGCCTACCG CATGTTTACA AACAACACAT GTTTGAAGCA CATGATCACC
    AAAGTCCGGC GGGACACCCA CCACTTTGAA CGCTACCAGC ATAACCGGGA CCTCGTGGGA
    TTCCTCAACA TGTTTGCAAA CAAACAACTG GAGCTGCCAA GGGGCTGGGA AATGAAACAC
    GATCATCAGG GCAAGGCATT TTTTGTTGAC CACAACTCCC GAACCACCAC TTTCATCGAT
    CCCCGACTCC CGCTTCAGAG CAGCAGACCC ACGAGCGCAC TGGTGCATCG ACAACACCTG
    ACCCGGCAGC GCAGCCACAG CGCAGGCGAG GTAGGAGAAG ATTCTCGACA TGCAGGACCA
    CCAGTTCTTC CCAGGCCGTC GAGTACGTTC AATACAGTCA GCAGGCCGCA GTACCAGGAC
    ATGGTTCCAG TGGCTTACAA TGACAAGATT GTTGCATTTC TGCGCCAGCC CAACATCTTT
    GAAATTCTGC AGGAACGTCA ACCTGATCTT ACCAGGAATC ACTCACTCAG GGAGAAGATC
    CAATTTATCC GAACTGAAGG GACTCCAGGA TTGGTGCGTC TTTCAAGTGA TGCAGACCTT
    GTCATGCTGC TGAGTTTATT TGAAGAAGAG ATAATGTCAT ATGTGCCTCC ACATGCCTTA
    CTCCATCCCA GCTACTGTCA GTCCCCACGT GGCTCCCCTG TGTCCTCTCC CCAGAACTCA
    CCAGGTACTC AGCGTGCCAA TGCCCGCGCT CCAGCCCCTT ACAAGCGAGA CTTTGAAGCC
    AAACTAAGGA ACTTTTACAG GAAGTTAGAG ACTAAAGGAT ATGGACAAGG CCCAGGGAAA
    TTAAAGTTAA TTATCAGAAG AGATCACTTG CTTGAAGATG CTTTTAATCA AATTATGGGC
    TACTCCAGAA AAGACCTGCA GAGAAATAAG CTGTACGTCA CATTCGTCGG AGAGGAAGGG
    CTGGATTACA GTGGACCTTC CAGAGAGTTT TTCTTCCTGG TATCCAGAGA ACTCTTTAAC
    CCATATTATG GCTTATTTGA ATATTCAGCC AATGACACAT ACACAGTACA AATAAGTCCC
    ATGTCTGCCT TTGTAGACAA TCACCATGAA TGGTTCCGGT TCAGTGGTAG GATCCTTGGC
    CTTGCACTAA TTCACCAGTA TTTGTTGGAT GCCTTCTTCA CACGGCCCTT TTATAAGGCT
    CTTCTCAGAA TTCTCTGTGA CCTGAGTGAT CTAGAATACC TTGATGAAGA GTTCCATCAG
    AGCCTGCAGT GGATGAAAGA CAATGACATC CATGACATCC TGGACCTCAC ATTCACTGTG
    AACGAAGAAG TTTTTGGGCA GATTACTGAA CGAGAATTAA AACCAGGGGG TGCCAACATC
    CCAGTCACAG AGAAGAACAA GAAGGAGTAC ATTGAGAGGA TGGTGAAATG GAGGATTGAG
    AGGGGTGTCG TGCAGCAGAC AGAGAGCTTA GTGCGTGGCT TCTATGAGGT GGTGGATGCC
    AGGCTGGTAT CTGTTTTTGA TGCAAGAGAA CTAGAACTGG TCATCGCAGG CACAGCTGAA
    ATAGACCTAA GTGATTGGAG AAACAACACA GAATACAGAG GAGGATATCA TGACAATCAC
    ATTGTAATTC GGTGGTTCTG GGCTGCAGTG GAAAGATTCA ACAATGAACA ACGACTGAGG
    TTGTTACAGT TTGTTACAGG CACATCCAGC ATTCCCTATG AAGGATTTGC TTCTCTCCGA
    GGCAGTAATG GCCCAAGAAG ATTCTGTGTG GAGAAATGGG GGAAAATCAC TGCTCTTCCC
    AGAGCTCACA CTTGTTTTAA CCGTCTGGAT CTCCCTCCAT ACCCATCCTT CTCCATGCTT
    TATGAAAAAC TCTTGACAGC AGTTGAAGAG ACCAGTACCT TTGGACTTGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa227758
    Clone ID Related Accession (Same CDS sequence) XM_024981178.1
    Accession Version XM_024981178.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product E3 ubiquitin-protein ligase HECW2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGCT CAGCCCGGGA ACACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
    TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACCGAGAAT
    ATGACCCTAC AGCGGGCCAA CAGCGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC
    TTGACGGCCA GCATGTATGA GTACACACTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG
    GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TGTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG
    AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT
    TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGACT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT
    AATCCAATTT GGCATCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG
    ATTGAAATTA AAGACAAGTT TGCAAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG
    CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG
    GAGGTCACAT CTTCTGTGCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA
    GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC
    CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA
    CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT
    ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT
    GCTATTGAAC ACAATGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG
    GGAGCCTCTC CAAAGCTAAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCAATGCTT
    CATATTGACT CAGATGAGGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC
    TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC
    CTGGCATCTC AGACAAAGGC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT
    GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA
    CCCTCAGGCC CGGTCCCAGA TGAAAGCGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC
    CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC
    ACGGGCACTT CTGACACGTC CGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTT
    GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGTGA CCCTGCCAGG
    ACAGAGAGTG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCT
    GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA
    TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT
    GGAGCCTGTG CAGCAGAGCC CGCCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA
    TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG
    TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG
    AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGAAGAGGG TGCTGCTCGG
    GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AAGGCGCGAT TGGAGGTTGT
    CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG
    TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC
    AGGATCTTCT ACGTGGATCA TGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC
    CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG
    CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT
    ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG
    AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC
    GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC
    CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC
    CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA
    AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA
    TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG
    AGCAGCAGAC CCACGAGCGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC
    AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG
    TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC
    AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT
    CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA
    GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA
    TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTATTGT
    CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC
    AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC
    AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA
    AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG
    CAGAGAAATA AGCTGTACGT CACATTCGTC GGAGAGGAAG GGCTGGATTA CAGTGGACCT
    TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT
    GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC
    AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GCCTTGCACT AATTCACCAG
    TATTTGTTGG ATGCCTTCTT CACACGGCCC TTTTATAAGG CTCTTCTCAG AATTCTCTGT
    GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA
    GACAATGACA TCCATGACAT CCTGGACCTC ACATTCACTG TGAACGAAGA AGTTTTTGGG
    CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC
    AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG
    ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT
    GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGATTGG
    AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC
    TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA
    GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA
    AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT
    AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA
    GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024836946.1
    CDS123..4838
    Translation

    Target ORF information:

    RefSeq Version XM_024981178.1
    Organism Bos taurus(cattle)
    Definition Bos taurus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024981178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGCT CAGCCCGGGA ACACCTGCTT TTTGTAAGGC GTCGAAATCC CCAGATGCGG 
    TACACCCTGA GTCCAGAGAA CCTCCAGAGC CTGGCTGCCC AGAGCTCCAT GACCGAGAAT
    ATGACCCTAC AGCGGGCCAA CAGCGACACA GATCTGGTGA CCTCTGAGAG CCGCTCCAGC
    TTGACGGCCA GCATGTATGA GTACACACTG GGGCAAGCCC AGAACCTCAT CATCTTCTGG
    GACATTAAAG AGGAGGTGGA TCCCAGTGAT TGGATAGGAC TGTATCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGTGTGA CTGGAACACA AAAAGGACAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAGC CCGAGATAAA AATCTGTTTC
    AAATACTACC ACGGCATCAG TGGAGCCCTG CGAGCCACGA CCCCTTGCAT CACGGTGAAG
    AACCCAGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GGGCTTCAGG AAATCAGCAT
    TCTCGGAAGC TTGTTAGCTT TACTTTGTCA GACCTTAGGG CAGTTGGACT AAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGGAGGTCTA CTATCATCAG TAACACCACT
    AATCCAATTT GGCATCGAGA GAAATACTCC TTTTTTGCAC TCCTTACTGA TGTCTTAGAG
    ATTGAAATTA AAGACAAGTT TGCAAAGAGC CGGCCTATCA TTAAGCGTTT CCTGGGGAAG
    CTCACCATTC CAGTCCAGAG GCTACTGGAG CGGCAAGCCA TTGGTGACCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCAGCTGAC CATGTGAGTG GGTACCTCCA GTTCAGAGTG
    GAGGTCACAT CTTCTGTGCA TGAAGATGCC TCTCCAGAAG CTGTTGGCAC AATACTTGGA
    GTCAGTTCTG TGAACGGAGA CCTAGGAAGC CCTTCTGATG AGGAGGACAT GCCAGGGGGC
    CATCACGACA GCCCAGTTTG TTCCAATGGA CCAGTTTCTG AGGAAAGTGC TGATGGGACA
    CCCAAGCATT CTCTCAGGAC TAGTTCTACT CTGGAAATAG ACACAGAAGA ATTAACTTCT
    ACTTCTTCAA GGACCTCACC TCCCAGAGGA CGTCAGGATT CACTCAATGA TTATTTAGAT
    GCTATTGAAC ACAATGGCCA TTCTAGGTCA GGTGCAGCTA CTTGCTCGGA GAGGTCCGTG
    GGAGCCTCTC CAAAGCTAAG GAGTAGTTTT CCCACTGACA CAAGACTCAA TGCAATGCTT
    CATATTGACT CAGATGAGGA AGACCACGAG TTCCAGCAAG ACCTGGGCTA TCCATCCTCC
    TTGGAGGAGG AAGGAGGGTT GATCATGTTT AGCAGGGCAT CAAGAACTGA TGATGGGAGC
    CTGGCATCTC AGACAAAGGC AGAAGACAAC CCAGTGGAGA CTGAGGAAGC CTCCATTAAT
    GAAGCTGCTT CCTTTGAGGA TAAGCCAGGA AATCTTCCAG AGCTTGCAGA GACCTCTTTA
    CCCTCAGGCC CGGTCCCAGA TGAAAGCGAG AATGGCCCAG AATCTCAACC AAGTGTGGAC
    CAGGGCAGCA CTGAATTGTG TGGCTCTCAG GAGGTGGATC AGCCCACAAG TGGAGCTGAC
    ACGGGCACTT CTGACACGTC CGGGGGGAGC CGAAGAGCTG TCAGTGAGAC AGAGTCCCTT
    GATCAGGGGT CTGAGCCTTC CCAGGTGTCC TCAGAAACAG AGCCCAGTGA CCCTGCCAGG
    ACAGAGAGTG TGAGCGAGGC CAGCACCAGG CCCGAGGGAG AGAGTGACCT GGAAGGGGCT
    GACAGCTCCT GCAATGAGAG TGTTACCACG CAGCTCTCCT CGGTGGAGAC ACGGTGCTCA
    TCTCTTGAGA GCGCACGGTT TCCTGAAACT CCAGCCTTTT CTTCTCAGGA GGAGGAAGAT
    GGAGCCTGTG CAGCAGAGCC CGCCAGCAGT GGCCCTGCTG AAGGGTCCCA GGATTCCATA
    TGCACTGCTG GTTCCTTACC TGTGGTGCAG GTGCCCAGTG GAGAGGAGGA AGGGCCAGGG
    TCAGAATCAA CCACTGTACC TGACCAGGAG GAGGTGGGGG AGGTCTGGCA GCGGAGGGGG
    AGCCTGGAGG GAGCTGCTGC TGCTGCTGAG AGCCAGCCCC AAGAAGAGGG TGCTGCTCGG
    GATACCCCAG GGGCTTGTGA AGGGGCCACC GCCCAGGAGG AAGGCGCGAT TGGAGGTTGT
    CAATCCAACG GCCACCAGCC ACTGCGATCG CTGCCTTCAG TTCGCCAGGA TGTTAGCCGG
    TACCAGAGGG TGGACGAGGC TCTCCCACCA AACTGGGAGG CGCGCATTGA CAGCCACGGC
    AGGATCTTCT ACGTGGATCA TGTAAACAGA ACCACGACGT GGCAGCGGCC CACAGCTCCC
    CCTGCCCCGC AGGTGCTGCA GAGGTCCAAC TCCATACAGC AGATGGAGCA GCTGAACCGG
    CGATACCAGA GTATCCGCAG AACCATGACC AACGAGAGGC CTGAGGAAAA TAGCAATGCT
    ATTGATGGGG CCGGGGAGGA AGCCGACTTT CACCAAGCCA GTGCAGATTT CCGACGGGAG
    AGCGTCCTGC CCCACTCTAC CTCCAGATCC AGGCTCACGT TGCTGTTACA GTCTCCCCCC
    GTGAAGTTCC TCATCAGCCC AGAGTTCTTC ACCGTGCTGC ATTCCAACCC TAGTGCCTAC
    CGCATGTTTA CAAACAACAC ATGTTTGAAG CACATGATCA CCAAAGTCCG GCGGGACACC
    CACCACTTTG AACGCTACCA GCATAACCGG GACCTCGTGG GATTCCTCAA CATGTTTGCA
    AACAAACAAC TGGAGCTGCC AAGGGGCTGG GAAATGAAAC ACGATCATCA GGGCAAGGCA
    TTTTTTGTTG ACCACAACTC CCGAACCACC ACTTTCATCG ATCCCCGACT CCCGCTTCAG
    AGCAGCAGAC CCACGAGCGC ACTGGTGCAT CGACAACACC TGACCCGGCA GCGCAGCCAC
    AGCGCAGGCG AGGTAGGAGA AGATTCTCGA CATGCAGGAC CACCAGTTCT TCCCAGGCCG
    TCGAGTACGT TCAATACAGT CAGCAGGCCG CAGTACCAGG ACATGGTTCC AGTGGCTTAC
    AATGACAAGA TTGTTGCATT TCTGCGCCAG CCCAACATCT TTGAAATTCT GCAGGAACGT
    CAACCTGATC TTACCAGGAA TCACTCACTC AGGGAGAAGA TCCAATTTAT CCGAACTGAA
    GGGACTCCAG GATTGGTGCG TCTTTCAAGT GATGCAGACC TTGTCATGCT GCTGAGTTTA
    TTTGAAGAAG AGATAATGTC ATATGTGCCT CCACATGCCT TACTCCATCC CAGCTATTGT
    CAGTCCCCAC GTGGCTCCCC TGTGTCCTCT CCCCAGAACT CACCAGGTAC TCAGCGTGCC
    AATGCCCGCG CTCCAGCCCC TTACAAGCGA GACTTTGAAG CCAAACTAAG GAACTTTTAC
    AGGAAGTTAG AGACTAAAGG ATATGGACAA GGCCCAGGGA AATTAAAGTT AATTATCAGA
    AGAGATCACT TGCTTGAAGA TGCTTTTAAT CAAATTATGG GCTACTCCAG AAAAGACCTG
    CAGAGAAATA AGCTGTACGT CACATTCGTC GGAGAGGAAG GGCTGGATTA CAGTGGACCT
    TCCAGAGAGT TTTTCTTCCT GGTATCCAGA GAACTCTTTA ACCCATATTA TGGCTTATTT
    GAATATTCAG CCAATGACAC ATACACAGTA CAAATAAGTC CCATGTCTGC CTTTGTAGAC
    AATCACCATG AATGGTTCCG GTTCAGTGGT AGGATCCTTG GCCTTGCACT AATTCACCAG
    TATTTGTTGG ATGCCTTCTT CACACGGCCC TTTTATAAGG CTCTTCTCAG AATTCTCTGT
    GACCTGAGTG ATCTAGAATA CCTTGATGAA GAGTTCCATC AGAGCCTGCA GTGGATGAAA
    GACAATGACA TCCATGACAT CCTGGACCTC ACATTCACTG TGAACGAAGA AGTTTTTGGG
    CAGATTACTG AACGAGAATT AAAACCAGGG GGTGCCAACA TCCCAGTCAC AGAGAAGAAC
    AAGAAGGAGT ACATTGAGAG GATGGTGAAA TGGAGGATTG AGAGGGGTGT CGTGCAGCAG
    ACAGAGAGCT TAGTGCGTGG CTTCTATGAG GTGGTGGATG CCAGGCTGGT ATCTGTTTTT
    GATGCAAGAG AACTAGAACT GGTCATCGCA GGCACAGCTG AAATAGACCT AAGTGATTGG
    AGAAACAACA CAGAATACAG AGGAGGATAT CATGACAATC ACATTGTAAT TCGGTGGTTC
    TGGGCTGCAG TGGAAAGATT CAACAATGAA CAACGACTGA GGTTGTTACA GTTTGTTACA
    GGCACATCCA GCATTCCCTA TGAAGGATTT GCTTCTCTCC GAGGCAGTAA TGGCCCAAGA
    AGATTCTGTG TGGAGAAATG GGGGAAAATC ACTGCTCTTC CCAGAGCTCA CACTTGTTTT
    AACCGTCTGG ATCTCCCTCC ATACCCATCC TTCTCCATGC TTTATGAAAA ACTCTTGACA
    GCAGTTGAAG AGACCAGTAC CTTTGGACTT GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.