hecw2b cDNA ORF clone, Danio rerio(zebrafish)

The following hecw2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hecw2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa05404 XM_003197612.4
Latest version!
Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b (hecw2b), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00
ODa147354 XM_003197612.5
Latest version!
Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b (hecw2b), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $797.30
$1139.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa05404
    Clone ID Related Accession (Same CDS sequence) XM_003197612.4
    Accession Version XM_003197612.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4419bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product E3 ubiquitin-protein ligase HECW2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003197612.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGACAG GCACCACCAC CACTGCCCGT GAGCATCTCC TGGTGGTGCG GAGGCGCAGC 
    CCACACATGC GCTACACCTT GAGCCCGGAA AACCTCCGCA GCCTTTCGGC GCAGGGTGGA
    TCTGGAGGAG GAGGCTCTAG AGGTGGAGCA CCCGAACCCA TGGGCCTGCA GCGGGCTAAC
    AGTGACACCG ATCTAGTGAC ATCGGACAGC CGTTCCTCCT TAACAGCATC TACATACCAG
    TTCACACTAG GGCGAGGGCA AAACCTGGTC ATCTCTTGGG ACATCAAGGA GGAGGTGGAT
    GCCACTGACT GGATTGGGCT CTATCACATT GATGAGACGT GTCCTGCTAA TGTTTGGGAC
    TCGAAGAACC GCGGGGTGAA CGGCACTCAG AGAGGGCAGA TCGTCTGGAG GCTGGAGCCC
    GGACCATACT TTATGGAGCC TGAGACCAAA ATCTGCTTTA AATATTACCA TGGAGTGAGC
    GGAGCACTGA GGGCCACGAC ACCCTGCATT ACTGTCAAGA ACCCTGGAGT TACTGTTGGG
    GCAGAGGGGC AGGTGGATGG ACAGTCTGTC ACTGAACACT GCAGGAAATT GGTCAGCTTT
    ACATTATCAG ATATCAGGGC TCAGGGTCTG AAGAAGGGCA TGTTTTTTAA TCCGGACCCG
    TATCTAAAAA TGAGCATCCA ACCAGGCAAG AGGAACGGCT TCCCCACGTT CAGCCACCAC
    GGCCAGGAGA GACGAACTTC CATCATATCC AACACCACCA ACCCTGTGTG GCACGGAGAG
    AAGTACACTT TTGTTGCACT GATGACAGAC GTTTTGGAAA TCGAGGTCAA GGACAAATTT
    GCAAAAAGTC GCCCGATCAT CAAACGCTTT TTAGGTCAGC TGATAATCCC GGTGCAGAGA
    CTGCTGGAGA GACACACAGC CGGGGATCAA CATGTGAGCT ACACACTTTG TCGTCGACTA
    CCAACAGAAC ATGTGAGCGG ACAACTTCAC TTCCGAGTGG AGATCACCTT CAACGGGCAT
    GAAGATGCCA TGGGGTCTCT GCTGGGAGCC TCCGCTATGA ATGGAGATCC TGGGAGCCCA
    TCGGACGACG AAGACGTTCT TCAACCTGTG TCCCGCATGG TCAGGGGCCC GTCGCCCACA
    GGCTCTGAAG GGTCTCTGCA TGGTGATGCT TTTAGGCCTG ATGAGTGCAT GATGGACCCT
    GAAGAGGATC GGCTCTTGAG AGACGGAGCC CCAGATCTGG CTGAAGGGGC CCCGGCTCAT
    ACTCATCGCC AGTCCTCGCT TAATGACTAC CTGGATGCCA TTGAGGCCCC CAAGCCCCCT
    GGGGACAGAC CGCTCGGGGC CTCCTCTCCT AAACTACGCT CAAGTTTCCC CACCGACACG
    CGGCTCAATG CAATGTTACA TATAGACTCT GATGAAGACG AGGAAGGACA CGCCTCCCGG
    GACACTGCAT TGACCAATGG AATGCCGCGG CCTGAAGGAC ACGCCTCCTC TGAAAGAGGC
    AAAGGGGAGG TGCATGTGAA GAACAGGGAA AGGCTTGAGT CAGAAGCGGC GAGCAGTGCT
    ACAGATACTG AAGCCACAGC ATCAGTGTCC AGCAGTGGCA CCACCAAGAG CCAAAATGGC
    GGAGGTGCTG AAGGCGGTCA GGAAGGAGCA GCTATTTCTG GAGACTTGAC CCAAAGCATC
    AGCAGCTCTC AGCAGCCAGT TCTTCGAATA CAGGATGAGG AAGGAGCTGC TCCCCAGGCA
    TCCTCCAGGA CTGAAGGATC ATCAGAGGCT CCTGCAGTCT CAGAGGCCTC AGCGTCTGCC
    ATTTATACAG TGAATGAAGT GGGCGGAGCC ACAGCCACAA ATCAGCCAGA ACGTATGAAC
    TCCAGTGAGA TGACCAATCA GGAAGAGGAG GATGAAGAAA TCTGGCAGAG GAGGCGGAGT
    CTGCAGGCAT CAGGGAACGA ATCACAGGCT GAAGCGATGG AATTAGGCAA TGAAGTGAGG
    AGAACGGCAG ATGAAGCGCA GGCTGCTTTG ATAACAGATG GAGAGACAGG CGCTTGTGCT
    CAAGTGAACG GCCACCAGCC GATGCGCTCT CTTCCTTCAG TTCGGCACGA CATCAGCCGC
    TATCAGAGAG TGGACGAGCC TCTTCCTCCA AACTGGGAGG CTCGTATTGA CAGCCATGGC
    AGGATTTTCT ACGTGGATCA TGTGAACCGA ACCACGACCT GGCAGCGTCC CACGGCTCCA
    CCTGCACCTC AGGTCCTGCA GAGATCAAAC TCCATTCAGC AGATGGAGCA GCTCAACCGC
    AGGTATCAGA GTATCCGGAG AACAATAACC AATGAGAGGA CAGAAGAACA GGCCGCAGAG
    ATGCCACCAG ACGACCTGAC AACATCTGAC CTGCTGCATC ACTCCATACC TGAGTACCGG
    CGGGACGGTG TTGTTGGACC CGCCAGCTCT CGTTCCCGCC TGACTCTGCT GCTTCAGTCC
    CCCAGCGCCA AATTTCTCAC CAGCCCGGAT TTCTTCACCG TACTGCATTC CAACCCTAGT
    GCCTACCGCA TGTTTACAAG TAACACGTGT CTGAAACACA TGATCAGTAA AGTGCGACGG
    GACGCGCATC ACTTCGAGCG CTACCAACAC AACCGAGACC TGGTTGCCTT CCTCAACATG
    TTCGCCAATA AACAGCTGGA TCTGCCGCGG GGCTGGGAAA TGAAACACGA CCATACGGGA
    AAGCCTTTCT TCGTGGATCA CAACTGCAGG GCGACCACAT TCATCGACCC ACGGCTGCCG
    CTGCAGAGCG CCCGTCCCAG CAGCCTTCTC GCCCACCGCC AGCATCTGAG CCGCCAGCGC
    AGCCACAGCG CAGGAGAGGT AGGCGACGAT CCTCGGCATG CTGGACCCGC TGTACTGCCA
    CGCCCCTCCA ACACCTTCAC AGCCAATCGC AACCAGTGTC AAGACTTGGT TCCTGTTGCC
    TATAATGAAA AAATAGTGGC ATTTCTGCGG CAGCCGAACA TATTTGATAT CCTTCAGGAG
    AGGCAGCCTG AGCTCATTAG GAATCACTCA CTCAGGGAGA AGGTGCAGTT CATTCGTAAT
    GAAGGAGCGT CAGGCTTGGC TCGTCTCTCC AGCGATGCAG ACCTGGTGAT TTTGCTCAGT
    TTGTTTGAAG AGGAAGTGAT GTCGTATGTG CCACCTCATG CCTTACTGCA CCCCAGCTAC
    TGCCAGTCAC CACGAGGGTC TCCAGTCTCC TCTCCTCAAA ACTCTCCAGG TACACAGAGA
    GCCAACGCTC GGGCCCCTGC ACCCTACAAA CGAGACTTTG AAGCCAAACT TCGAAATTTC
    TATCGCAAGC TTGAGACTAA AGGATACGGA CAGGGTCCTG GTAAAGTCAA GCTGATAATC
    CGCAGAGATC ATCTACTGGA GGATGCCTTC AATCAGATCA TGTGCTACTC TCGAAAAGAT
    CTCCAGAGAA GCAAACTCTA CGTCAGCTTT GTTGGAGAAG AAGGATTGGA CTACAGTGGC
    CCGTCAAGAG AGTTTTTCTT TCTGGTGTCG AGGGAGCTCT TTAACCCTTA TTATGGTTTG
    TTTGAGTATT CAGCCAACGA CACCTACACC GTTCAGATCA GTCCCATGTC TGCCTTCGTT
    GACAATCATC ATGAATGGTT TCGCTTCAGC GGCCGTATTC TAGGTCTCGC TCTTATCCAT
    CAATACCTAC TGGATGCCTT CTTCACACGA CCCTTCTACA AAGGCCTGTT GAGAATCCCG
    TGTGATCTGA GTGATCTGGA GTATCTGGAT GAGGAGTTTC ATCAGAGTCT TCAGTGGATG
    AAGGACAATG ACATAGAAGA TATGCTGGAT CTGACCTTCA CAGTCAATGA GGAGGTGTTC
    GGACAGATCA CAGAGCGCGA GCTGAAGCCT GGCGGTGCAA ACATCCCAGT GTCGGAGAAG
    AATAAGAAGG AGTACATCGA ACGCATGGTG AAGTGGCGTA TCGAGCGCGG CGTCGTCCAG
    CAGACCGAGA GTCTCGTACG GGGCTTTTAT GAGGTTGTAG ATGCGCGGCT GGTGTCTGTG
    TTTGATGCAC GAGAGCTGGA GCTGGTGATC GCTGGGACAG CAGAGATTGA TCTGGGAGAC
    TGGAGGAACA ACACTGAATA CAGAGGAGGT TATCATGATA ACCACATCGT GATCCGCTGG
    TTCTGGGCGG CAGTGGAGCG ATTCAACAAT GAGCAGAGAC TCCGGTTACT CCAGTTTGTG
    ACCGGGACGT CCAGTATCCC GTATGAGGGC TTTGCCTCGC TCAGGGGCAG CAATGGGCCG
    CGGCGCTTCT GTGTGGAGAA ATGGGGGAAA ATCACATCTC TGCCCAGGGC TCACACGTGT
    TTCAACCGGC TGGATCTTCC CCCCTATCCT TCATTCTCCA TGCTCTACGA GAAGATGCTG
    ACGGCAGTGG AGGAGACCAG CACTTTCGGT TTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003197660.2
    CDS2362..6780
    Translation

    Target ORF information:

    RefSeq Version XM_003197612.4
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b (hecw2b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003197612.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGACAG GCACCACCAC CACTGCCCGT GAGCATCTCC TGGTGGTGCG GAGGCGCAGC 
    CCACACATGC GCTACACCTT GAGCCCGGAA AACCTCCGCA GCCTTTCGGC GCAGGGTGGA
    TCTGGAGGAG GAGGCTCTAG AGGTGGAGCA CCCGAACCCA TGGGCCTGCA GCGGGCTAAC
    AGTGACACCG ATCTAGTGAC ATCGGACAGC CGTTCCTCCT TAACAGCATC TACATACCAG
    TTCACACTAG GGCGAGGGCA AAACCTGGTC ATCTCTTGGG ACATCAAGGA GGAGGTGGAT
    GCCACTGACT GGATTGGGCT CTATCACATT GATGAGACGT GTCCTGCTAA TGTTTGGGAC
    TCGAAGAACC GCGGGGTGAA CGGCACTCAG AGAGGGCAGA TCGTCTGGAG GCTGGAGCCC
    GGACCATACT TTATGGAGCC TGAGACCAAA ATCTGCTTTA AATATTACCA TGGAGTGAGC
    GGAGCACTGA GGGCCACGAC ACCCTGCATT ACTGTCAAGA ACCCTGGAGT TACTGTTGGG
    GCAGAGGGGC AGGTGGATGG ACAGTCTGTC ACTGAACACT GCAGGAAATT GGTCAGCTTT
    ACATTATCAG ATATCAGGGC TCAGGGTCTG AAGAAGGGCA TGTTTTTTAA TCCGGACCCG
    TATCTAAAAA TGAGCATCCA ACCAGGCAAG AGGAACGGCT TCCCCACGTT CAGCCACCAC
    GGCCAGGAGA GACGAACTTC CATCATATCC AACACCACCA ACCCTGTGTG GCACGGAGAG
    AAGTACACTT TTGTTGCACT GATGACAGAC GTTTTGGAAA TCGAGGTCAA GGACAAATTT
    GCAAAAAGTC GCCCGATCAT CAAACGCTTT TTAGGTCAGC TGATAATCCC GGTGCAGAGA
    CTGCTGGAGA GACACACAGC CGGGGATCAA CATGTGAGCT ACACACTTTG TCGTCGACTA
    CCAACAGAAC ATGTGAGCGG ACAACTTCAC TTCCGAGTGG AGATCACCTT CAACGGGCAT
    GAAGATGCCA TGGGGTCTCT GCTGGGAGCC TCCGCTATGA ATGGAGATCC TGGGAGCCCA
    TCGGACGACG AAGACGTTCT TCAACCTGTG TCCCGCATGG TCAGGGGCCC GTCGCCCACA
    GGCTCTGAAG GGTCTCTGCA TGGTGATGCT TTTAGGCCTG ATGAGTGCAT GATGGACCCT
    GAAGAGGATC GGCTCTTGAG AGACGGAGCC CCAGATCTGG CTGAAGGGGC CCCGGCTCAT
    ACTCATCGCC AGTCCTCGCT TAATGACTAC CTGGATGCCA TTGAGGCCCC CAAGCCCCCT
    GGGGACAGAC CGCTCGGGGC CTCCTCTCCT AAACTACGCT CAAGTTTCCC CACCGACACG
    CGGCTCAATG CAATGTTACA TATAGACTCT GATGAAGACG AGGAAGGACA CGCCTCCCGG
    GACACTGCAT TGACCAATGG AATGCCGCGG CCTGAAGGAC ACGCCTCCTC TGAAAGAGGC
    AAAGGGGAGG TGCATGTGAA GAACAGGGAA AGGCTTGAGT CAGAAGCGGC GAGCAGTGCT
    ACAGATACTG AAGCCACAGC ATCAGTGTCC AGCAGTGGCA CCACCAAGAG CCAAAATGGC
    GGAGGTGCTG AAGGCGGTCA GGAAGGAGCA GCTATTTCTG GAGACTTGAC CCAAAGCATC
    AGCAGCTCTC AGCAGCCAGT TCTTCGAATA CAGGATGAGG AAGGAGCTGC TCCCCAGGCA
    TCCTCCAGGA CTGAAGGATC ATCAGAGGCT CCTGCAGTCT CAGAGGCCTC AGCGTCTGCC
    ATTTATACAG TGAATGAAGT GGGCGGAGCC ACAGCCACAA ATCAGCCAGA ACGTATGAAC
    TCCAGTGAGA TGACCAATCA GGAAGAGGAG GATGAAGAAA TCTGGCAGAG GAGGCGGAGT
    CTGCAGGCAT CAGGGAACGA ATCACAGGCT GAAGCGATGG AATTAGGCAA TGAAGTGAGG
    AGAACGGCAG ATGAAGCGCA GGCTGCTTTG ATAACAGATG GAGAGACAGG CGCTTGTGCT
    CAAGTGAACG GCCACCAGCC GATGCGCTCT CTTCCTTCAG TTCGGCACGA CATCAGCCGC
    TATCAGAGAG TGGACGAGCC TCTTCCTCCA AACTGGGAGG CTCGTATTGA CAGCCATGGC
    AGGATTTTCT ACGTGGATCA TGTGAACCGA ACCACGACCT GGCAGCGTCC CACGGCTCCA
    CCTGCACCTC AGGTCCTGCA GAGATCAAAC TCCATTCAGC AGATGGAGCA GCTCAACCGC
    AGGTATCAGA GTATCCGGAG AACAATAACC AATGAGAGGA CAGAAGAACA GGCCGCAGAG
    ATGCCACCAG ACGACCTGAC AACATCTGAC CTGCTGCATC ACTCCATACC TGAGTACCGG
    CGGGACGGTG TTGTTGGACC CGCCAGCTCT CGTTCCCGCC TGACTCTGCT GCTTCAGTCC
    CCCAGCGCCA AATTTCTCAC CAGCCCGGAT TTCTTCACCG TACTGCATTC CAACCCTAGT
    GCCTACCGCA TGTTTACAAG TAACACGTGT CTGAAACACA TGATCAGTAA AGTGCGACGG
    GACGCGCATC ACTTCGAGCG CTACCAACAC AACCGAGACC TGGTTGCCTT CCTCAACATG
    TTCGCCAATA AACAGCTGGA TCTGCCGCGG GGCTGGGAAA TGAAACACGA CCATACGGGA
    AAGCCTTTCT TCGTGGATCA CAACTGCAGG GCGACCACAT TCATCGACCC ACGGCTGCCG
    CTGCAGAGCG CCCGTCCCAG CAGCCTTCTC GCCCACCGCC AGCATCTGAG CCGCCAGCGC
    AGCCACAGCG CAGGAGAGGT AGGCGACGAT CCTCGGCATG CTGGACCCGC TGTACTGCCA
    CGCCCCTCCA ACACCTTCAC AGCCAATCGC AACCAGTGTC AAGACTTGGT TCCTGTTGCC
    TATAATGAAA AAATAGTGGC ATTTCTGCGG CAGCCGAACA TATTTGATAT CCTTCAGGAG
    AGGCAGCCTG AGCTCATTAG GAATCACTCA CTCAGGGAGA AGGTGCAGTT CATTCGTAAT
    GAAGGAGCGT CAGGCTTGGC TCGTCTCTCC AGCGATGCAG ACCTGGTGAT TTTGCTCAGT
    TTGTTTGAAG AGGAAGTGAT GTCGTATGTG CCACCTCATG CCTTACTGCA CCCCAGCTAC
    TGCCAGTCAC CACGAGGGTC TCCAGTCTCC TCTCCTCAAA ACTCTCCAGG TACACAGAGA
    GCCAACGCTC GGGCCCCTGC ACCCTACAAA CGAGACTTTG AAGCCAAACT TCGAAATTTC
    TATCGCAAGC TTGAGACTAA AGGATACGGA CAGGGTCCTG GTAAAGTCAA GCTGATAATC
    CGCAGAGATC ATCTACTGGA GGATGCCTTC AATCAGATCA TGTGCTACTC TCGAAAAGAT
    CTCCAGAGAA GCAAACTCTA CGTCAGCTTT GTTGGAGAAG AAGGATTGGA CTACAGTGGC
    CCGTCAAGAG AGTTTTTCTT TCTGGTGTCG AGGGAGCTCT TTAACCCTTA TTATGGTTTG
    TTTGAGTATT CAGCCAACGA CACCTACACC GTTCAGATCA GTCCCATGTC TGCCTTCGTT
    GACAATCATC ATGAATGGTT TCGCTTCAGC GGCCGTATTC TAGGTCTCGC TCTTATCCAT
    CAATACCTAC TGGATGCCTT CTTCACACGA CCCTTCTACA AAGGCCTGTT GAGAATCCCG
    TGTGATCTGA GTGATCTGGA GTATCTGGAT GAGGAGTTTC ATCAGAGTCT TCAGTGGATG
    AAGGACAATG ACATAGAAGA TATGCTGGAT CTGACCTTCA CAGTCAATGA GGAGGTGTTC
    GGACAGATCA CAGAGCGCGA GCTGAAGCCT GGCGGTGCAA ACATCCCAGT GTCGGAGAAG
    AATAAGAAGG AGTACATCGA ACGCATGGTG AAGTGGCGTA TCGAGCGCGG CGTCGTCCAG
    CAGACCGAGA GTCTCGTACG GGGCTTTTAT GAGGTTGTAG ATGCGCGGCT GGTGTCTGTG
    TTTGATGCAC GAGAGCTGGA GCTGGTGATC GCTGGGACAG CAGAGATTGA TCTGGGAGAC
    TGGAGGAACA ACACTGAATA CAGAGGAGGT TATCATGATA ACCACATCGT GATCCGCTGG
    TTCTGGGCGG CAGTGGAGCG ATTCAACAAT GAGCAGAGAC TCCGGTTACT CCAGTTTGTG
    ACCGGGACGT CCAGTATCCC GTATGAGGGC TTTGCCTCGC TCAGGGGCAG CAATGGGCCG
    CGGCGCTTCT GTGTGGAGAA ATGGGGGAAA ATCACATCTC TGCCCAGGGC TCACACGTGT
    TTCAACCGGC TGGATCTTCC CCCCTATCCT TCATTCTCCA TGCTCTACGA GAAGATGCTG
    ACGGCAGTGG AGGAGACCAG CACTTTCGGT TTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa147354
    Clone ID Related Accession (Same CDS sequence) XM_003197612.5
    Accession Version XM_003197612.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4419bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product E3 ubiquitin-protein ligase HECW2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003197612.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGACAG GCACCACCAC CACTGCCCGT GAGCATCTCC TGGTGGTGCG GAGGCGCAGC 
    CCACACATGC GCTACACCTT GAGCCCGGAA AACCTCCGCA GCCTTTCGGC GCAGGGTGGA
    TCTGGAGGAG GAGGCTCTAG AGGTGGAGCA CCCGAACCCA TGGGCCTGCA GCGGGCTAAC
    AGTGACACCG ATCTAGTGAC ATCGGACAGC CGTTCCTCCT TAACAGCATC TACATACCAG
    TTCACACTAG GGCGAGGGCA AAACCTGGTC ATCTCTTGGG ACATCAAGGA GGAGGTGGAT
    GCCACTGACT GGATTGGGCT CTATCACATT GATGAGACGT GTCCTGCTAA TGTTTGGGAC
    TCGAAGAACC GCGGGGTGAA CGGCACTCAG AGAGGGCAGA TCGTCTGGAG GCTGGAGCCC
    GGACCATACT TTATGGAGCC TGAGACCAAA ATCTGCTTTA AATATTACCA TGGAGTGAGC
    GGAGCACTGA GGGCCACGAC ACCCTGCATT ACTGTCAAGA ACCCTGGAGT TACTGTTGGG
    GCAGAGGGGC AGGTGGATGG ACAGTCTGTC ACTGAACACT GCAGGAAATT GGTCAGCTTT
    ACATTATCAG ATATCAGGGC TCAGGGTCTG AAGAAGGGCA TGTTTTTTAA TCCGGACCCG
    TATCTAAAAA TGAGCATCCA ACCAGGCAAG AGGAACGGCT TCCCCACGTT CAGCCACCAC
    GGCCAGGAGA GACGAACTTC CATCATATCC AACACCACCA ACCCTGTGTG GCACGGAGAG
    AAGTACACTT TTGTTGCACT GATGACAGAC GTTTTGGAAA TCGAGGTCAA GGACAAATTT
    GCAAAAAGTC GCCCGATCAT CAAACGCTTT TTAGGTCAGC TGATAATCCC GGTGCAGAGA
    CTGCTGGAGA GACACACAGC CGGGGATCAA CATGTGAGCT ACACACTTTG TCGTCGACTA
    CCAACAGAAC ATGTGAGCGG ACAACTTCAC TTCCGAGTGG AGATCACCTT CAACGGGCAT
    GAAGATGCCA TGGGGTCTCT GCTGGGAGCC TCCGCTATGA ATGGAGATCC TGGGAGCCCA
    TCGGACGACG AAGACGTTCT TCAACCTGTG TCCCGCATGG TCAGGGGCCC GTCGCCCACA
    GGCTCTGAAG GGTCTCTGCA TGGTGATGCT TTTAGGCCTG ATGAGTGCAT GATGGACCCT
    GAAGAGGATC GGCTCTTGAG AGACGGAGCC CCAGATCTGG CTGAAGGGGC CCCGGCTCAT
    ACTCATCGCC AGTCCTCGCT TAATGACTAC CTGGATGCCA TTGAGGCCCC CAAGCCCCCT
    GGGGACAGAC CGCTCGGGGC CTCCTCTCCT AAACTACGCT CAAGTTTCCC CACCGACACG
    CGGCTCAATG CAATGTTACA TATAGACTCT GATGAAGACG AGGAAGGACA CGCCTCCCGG
    GACACTGCAT TGACCAATGG AATGCCGCGG CCTGAAGGAC ACGCCTCCTC TGAAAGAGGC
    AAAGGGGAGG TGCATGTGAA GAACAGGGAA AGGCTTGAGT CAGAAGCGGC GAGCAGTGCT
    ACAGATACTG AAGCCACAGC ATCAGTGTCC AGCAGTGGCA CCACCAAGAG CCAAAATGGC
    GGAGGTGCTG AAGGCGGTCA GGAAGGAGCA GCTATTTCTG GAGGCTTGAC CCAAAGCATC
    AGCAGCTTTC AGCAGCCAGC TCTTCGAATA CAGGATGAGG AAGGAGCTGC TCCCCAGGCA
    TCCTCCAGGA CTGAAGGATC ATCAGAGGCT CCTGCAGTCT CAGAGGCCTC AGCGTCTGCC
    ATTTATACAG TGAATGAAGT GGGCGGAGCC ACAGCCACAA ATCAGCCAGA ACGTATGAAC
    TCCAGTGAGA TGACCAATCA GGAAGAGGAG GATGAAGAAA TCTGGCAGAG GAGGCGGAGT
    CTGCAGGCAT CAGGGAACGA ATCACAGGCT GAAGCGATGG AATTAGGCAA TGAAGTGAGG
    AGAACGGCAG ATGAAGCGCA GGCTGCTTTG ATAACAGATG GAGAGACAGG CGCTTGTGCT
    CAAGTGAACG GCCACCAGCC GATGCGCTCT CTTCCTTCAG TTCGGCACGA CATCAGCCGC
    TATCAGAGAG TGGACGAGCC TCTTCCTCCA AACTGGGAGG CTCGTATTGA CAGCCATGGC
    AGGATTTTCT ACGTGGATCA TGTGAACCGA ACCACGACCT GGCAGCGTCC CACGGCTCCA
    CCTGCACCTC AGGTCCTGCA GAGATCAAAC TCCATTCAGC AGATGGAGCA GCTCAACCGC
    AGGTATCAGA GTATCCGGAG AACAATAACC AATGAGAGGA CAGAAGAACA GGCCGCAGAG
    ATGCCACCAG ACGACCTGAC AACATCTGAC CTGCTGCATC ACTCCATACC TGAGTACCGG
    CGGGACGGTG TTGTTGGACC CGCCAGCTCT CGTTCCCGCC TGACTCTGCT GCTTCAGTCC
    CCCAGCGCCA AATTTCTCAC CAGCCCGGAT TTCTTCACCG TACTGCATTC CAACCCTAGT
    GCCTACCGCA TGTTTACAAG TAACACGTGT CTGAAACACA TGATCAGTAA AGTGCGACGG
    GACGCGCATC ACTTCGAGCG CTACCAACAC AACCGAGACC TGGTTGCCTT CCTCAACATG
    TTCGCCAATA AACAGCTGGA TCTGCCGCGG GGCTGGGAAA TGAAACACGA CCATACGGGA
    AAGCCTTTCT TCGTGGATCA CAACTGCAGG GCGACCACAT TCATCGACCC ACGGCTGCCG
    CTGCAGAGCG CCCGTCCCAG CAGCCTTCTC GCCCACCGCC AGCATCTGAG CCGCCAGCGC
    AGCCACAGCG CAGGAGAGGT AGGCGACGAT CCTCGGCATG CTGGACCCGC TGTACTGCCA
    CGCCCCTCCA ACACCTTCAC AGCCAATCGC AACCAGTGTC AAGACTTGGT TCCTGTTGCC
    TATAATGAAA AAATAGTGGC ATTTCTGCGG CAGCCGAACA TATTTGATAT CCTTCAGGAG
    AGGCAGCCTG AGCTCATTAG GAATCACTCA CTCAGGGAGA AGGTGCAGTT CATTCGTAAT
    GAAGGAGCGT CAGGCTTGGC TCGTCTCTCC AGCGATGCAG ACCTGGTGAT TTTGCTCAGT
    TTGTTTGAAG AGGAAGTGAT GTCGTATGTG CCACCTCATG CCTTACTGCA CCCCAGCTAC
    TGCCAGTCAC CACGAGGGTC TCCAGTCTCC TCTCCTCAAA ACTCTCCAGG TACACAGAGA
    GCCAACGCTC GGGCCCCTGC ACCCTACAAA CGAGACTTTG AAGCCAAACT TCGAAATTTC
    TATCGCAAGC TTGAGACTAA AGGATACGGA CAGGGTCCTG GTAAAGTCAA GCTGATAATC
    CGCAGAGATC ATCTACTGGA GGATGCCTTC AATCAGATCA TGTGCTACTC TCGAAAAGAT
    CTCCAGAGAA GCAAACTCTA CGTCAGCTTT GTTGGAGAAG AAGGATTGGA CTACAGTGGC
    CCGTCAAGAG AGTTTTTCTT TCTGGTGTCG AGGGAGCTCT TTAACCCTTA TTATGGTTTG
    TTTGAGTATT CAGCCAACGA CACCTACACC GTTCAGATCA GTCCCATGTC TGCCTTCGTT
    GACAATCATC ATGAATGGTT TCGCTTCAGC GGCCGTATTC TAGGTCTCGC TCTTATCCAT
    CAATACCTAC TGGATGCCTT CTTCACACGA CCCTTCTACA AAGGCCTGTT GAGAATCCCG
    TGTGATCTGA GTGATCTGGA GTATCTGGAT GAGGAGTTTC ATCAGAGTCT TCAGTGGATG
    AAGGACAATG ACATAGAAGA TATGCTGGAT CTGACCTTCA CAGTCAATGA GGAGGTGTTC
    GGACAGATCA CAGAGCGCGA GCTGAAGCCT GGCGGTGCAA ACATCCCAGT GTCGGAGAAG
    AATAAGAAGG AGTACATCGA ACGCATGGTG AAGTGGCGTA TCGAGCGCGG CGTCGTCCAG
    CAGACCGAGA GTCTCGTACG GGGCTTTTAT GAGGTTGTAG ATGCGCGGCT GGTGTCTGTG
    TTTGATGCAC GAGAGCTGGA GCTGGTGATC GCTGGGACAG CAGAGATTGA TCTGGGAGAC
    TGGAGGAACA ACACTGAATA CAGAGGAGGT TATCATGATA ACCACATCGT GATCCGCTGG
    TTCTGGGCGG CAGTGGAGCG ATTCAACAAT GAGCAGAGAC TCCGGTTACT CCAGTTTGTG
    ACCGGGACGT CCAGTATCCC GTATGAGGGC TTTGCCTCGC TCAGGGGCAG CAATGGGCCG
    CGGCGCTTCT GTGTGGAGAA ATGGGGGAAA ATCACATCTC TGCCCAGGGC TCACACGTGT
    TTCAACCGGC TGGATCTTCC CCCCTATCCT TCATTCTCCA TGCTCTACGA GAAGATGCTG
    ACGGCAGTGG AGGAGACCAG CACTTTCGGT TTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003197660.3
    CDS2364..6782
    Translation

    Target ORF information:

    RefSeq Version XM_003197612.5
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2b (hecw2b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003197612.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    ATGGCGACAG GCACCACCAC CACTGCCCGT GAGCATCTCC TGGTGGTGCG GAGGCGCAGC 
    CCACACATGC GCTACACCTT GAGCCCGGAA AACCTCCGCA GCCTTTCGGC GCAGGGTGGA
    TCTGGAGGAG GAGGCTCTAG AGGTGGAGCA CCCGAACCCA TGGGCCTGCA GCGGGCTAAC
    AGTGACACCG ATCTAGTGAC ATCGGACAGC CGTTCCTCCT TAACAGCATC TACATACCAG
    TTCACACTAG GGCGAGGGCA AAACCTGGTC ATCTCTTGGG ACATCAAGGA GGAGGTGGAT
    GCCACTGACT GGATTGGGCT CTATCACATT GATGAGACGT GTCCTGCTAA TGTTTGGGAC
    TCGAAGAACC GCGGGGTGAA CGGCACTCAG AGAGGGCAGA TCGTCTGGAG GCTGGAGCCC
    GGACCATACT TTATGGAGCC TGAGACCAAA ATCTGCTTTA AATATTACCA TGGAGTGAGC
    GGAGCACTGA GGGCCACGAC ACCCTGCATT ACTGTCAAGA ACCCTGGAGT TACTGTTGGG
    GCAGAGGGGC AGGTGGATGG ACAGTCTGTC ACTGAACACT GCAGGAAATT GGTCAGCTTT
    ACATTATCAG ATATCAGGGC TCAGGGTCTG AAGAAGGGCA TGTTTTTTAA TCCGGACCCG
    TATCTAAAAA TGAGCATCCA ACCAGGCAAG AGGAACGGCT TCCCCACGTT CAGCCACCAC
    GGCCAGGAGA GACGAACTTC CATCATATCC AACACCACCA ACCCTGTGTG GCACGGAGAG
    AAGTACACTT TTGTTGCACT GATGACAGAC GTTTTGGAAA TCGAGGTCAA GGACAAATTT
    GCAAAAAGTC GCCCGATCAT CAAACGCTTT TTAGGTCAGC TGATAATCCC GGTGCAGAGA
    CTGCTGGAGA GACACACAGC CGGGGATCAA CATGTGAGCT ACACACTTTG TCGTCGACTA
    CCAACAGAAC ATGTGAGCGG ACAACTTCAC TTCCGAGTGG AGATCACCTT CAACGGGCAT
    GAAGATGCCA TGGGGTCTCT GCTGGGAGCC TCCGCTATGA ATGGAGATCC TGGGAGCCCA
    TCGGACGACG AAGACGTTCT TCAACCTGTG TCCCGCATGG TCAGGGGCCC GTCGCCCACA
    GGCTCTGAAG GGTCTCTGCA TGGTGATGCT TTTAGGCCTG ATGAGTGCAT GATGGACCCT
    GAAGAGGATC GGCTCTTGAG AGACGGAGCC CCAGATCTGG CTGAAGGGGC CCCGGCTCAT
    ACTCATCGCC AGTCCTCGCT TAATGACTAC CTGGATGCCA TTGAGGCCCC CAAGCCCCCT
    GGGGACAGAC CGCTCGGGGC CTCCTCTCCT AAACTACGCT CAAGTTTCCC CACCGACACG
    CGGCTCAATG CAATGTTACA TATAGACTCT GATGAAGACG AGGAAGGACA CGCCTCCCGG
    GACACTGCAT TGACCAATGG AATGCCGCGG CCTGAAGGAC ACGCCTCCTC TGAAAGAGGC
    AAAGGGGAGG TGCATGTGAA GAACAGGGAA AGGCTTGAGT CAGAAGCGGC GAGCAGTGCT
    ACAGATACTG AAGCCACAGC ATCAGTGTCC AGCAGTGGCA CCACCAAGAG CCAAAATGGC
    GGAGGTGCTG AAGGCGGTCA GGAAGGAGCA GCTATTTCTG GAGGCTTGAC CCAAAGCATC
    AGCAGCTTTC AGCAGCCAGC TCTTCGAATA CAGGATGAGG AAGGAGCTGC TCCCCAGGCA
    TCCTCCAGGA CTGAAGGATC ATCAGAGGCT CCTGCAGTCT CAGAGGCCTC AGCGTCTGCC
    ATTTATACAG TGAATGAAGT GGGCGGAGCC ACAGCCACAA ATCAGCCAGA ACGTATGAAC
    TCCAGTGAGA TGACCAATCA GGAAGAGGAG GATGAAGAAA TCTGGCAGAG GAGGCGGAGT
    CTGCAGGCAT CAGGGAACGA ATCACAGGCT GAAGCGATGG AATTAGGCAA TGAAGTGAGG
    AGAACGGCAG ATGAAGCGCA GGCTGCTTTG ATAACAGATG GAGAGACAGG CGCTTGTGCT
    CAAGTGAACG GCCACCAGCC GATGCGCTCT CTTCCTTCAG TTCGGCACGA CATCAGCCGC
    TATCAGAGAG TGGACGAGCC TCTTCCTCCA AACTGGGAGG CTCGTATTGA CAGCCATGGC
    AGGATTTTCT ACGTGGATCA TGTGAACCGA ACCACGACCT GGCAGCGTCC CACGGCTCCA
    CCTGCACCTC AGGTCCTGCA GAGATCAAAC TCCATTCAGC AGATGGAGCA GCTCAACCGC
    AGGTATCAGA GTATCCGGAG AACAATAACC AATGAGAGGA CAGAAGAACA GGCCGCAGAG
    ATGCCACCAG ACGACCTGAC AACATCTGAC CTGCTGCATC ACTCCATACC TGAGTACCGG
    CGGGACGGTG TTGTTGGACC CGCCAGCTCT CGTTCCCGCC TGACTCTGCT GCTTCAGTCC
    CCCAGCGCCA AATTTCTCAC CAGCCCGGAT TTCTTCACCG TACTGCATTC CAACCCTAGT
    GCCTACCGCA TGTTTACAAG TAACACGTGT CTGAAACACA TGATCAGTAA AGTGCGACGG
    GACGCGCATC ACTTCGAGCG CTACCAACAC AACCGAGACC TGGTTGCCTT CCTCAACATG
    TTCGCCAATA AACAGCTGGA TCTGCCGCGG GGCTGGGAAA TGAAACACGA CCATACGGGA
    AAGCCTTTCT TCGTGGATCA CAACTGCAGG GCGACCACAT TCATCGACCC ACGGCTGCCG
    CTGCAGAGCG CCCGTCCCAG CAGCCTTCTC GCCCACCGCC AGCATCTGAG CCGCCAGCGC
    AGCCACAGCG CAGGAGAGGT AGGCGACGAT CCTCGGCATG CTGGACCCGC TGTACTGCCA
    CGCCCCTCCA ACACCTTCAC AGCCAATCGC AACCAGTGTC AAGACTTGGT TCCTGTTGCC
    TATAATGAAA AAATAGTGGC ATTTCTGCGG CAGCCGAACA TATTTGATAT CCTTCAGGAG
    AGGCAGCCTG AGCTCATTAG GAATCACTCA CTCAGGGAGA AGGTGCAGTT CATTCGTAAT
    GAAGGAGCGT CAGGCTTGGC TCGTCTCTCC AGCGATGCAG ACCTGGTGAT TTTGCTCAGT
    TTGTTTGAAG AGGAAGTGAT GTCGTATGTG CCACCTCATG CCTTACTGCA CCCCAGCTAC
    TGCCAGTCAC CACGAGGGTC TCCAGTCTCC TCTCCTCAAA ACTCTCCAGG TACACAGAGA
    GCCAACGCTC GGGCCCCTGC ACCCTACAAA CGAGACTTTG AAGCCAAACT TCGAAATTTC
    TATCGCAAGC TTGAGACTAA AGGATACGGA CAGGGTCCTG GTAAAGTCAA GCTGATAATC
    CGCAGAGATC ATCTACTGGA GGATGCCTTC AATCAGATCA TGTGCTACTC TCGAAAAGAT
    CTCCAGAGAA GCAAACTCTA CGTCAGCTTT GTTGGAGAAG AAGGATTGGA CTACAGTGGC
    CCGTCAAGAG AGTTTTTCTT TCTGGTGTCG AGGGAGCTCT TTAACCCTTA TTATGGTTTG
    TTTGAGTATT CAGCCAACGA CACCTACACC GTTCAGATCA GTCCCATGTC TGCCTTCGTT
    GACAATCATC ATGAATGGTT TCGCTTCAGC GGCCGTATTC TAGGTCTCGC TCTTATCCAT
    CAATACCTAC TGGATGCCTT CTTCACACGA CCCTTCTACA AAGGCCTGTT GAGAATCCCG
    TGTGATCTGA GTGATCTGGA GTATCTGGAT GAGGAGTTTC ATCAGAGTCT TCAGTGGATG
    AAGGACAATG ACATAGAAGA TATGCTGGAT CTGACCTTCA CAGTCAATGA GGAGGTGTTC
    GGACAGATCA CAGAGCGCGA GCTGAAGCCT GGCGGTGCAA ACATCCCAGT GTCGGAGAAG
    AATAAGAAGG AGTACATCGA ACGCATGGTG AAGTGGCGTA TCGAGCGCGG CGTCGTCCAG
    CAGACCGAGA GTCTCGTACG GGGCTTTTAT GAGGTTGTAG ATGCGCGGCT GGTGTCTGTG
    TTTGATGCAC GAGAGCTGGA GCTGGTGATC GCTGGGACAG CAGAGATTGA TCTGGGAGAC
    TGGAGGAACA ACACTGAATA CAGAGGAGGT TATCATGATA ACCACATCGT GATCCGCTGG
    TTCTGGGCGG CAGTGGAGCG ATTCAACAAT GAGCAGAGAC TCCGGTTACT CCAGTTTGTG
    ACCGGGACGT CCAGTATCCC GTATGAGGGC TTTGCCTCGC TCAGGGGCAG CAATGGGCCG
    CGGCGCTTCT GTGTGGAGAA ATGGGGGAAA ATCACATCTC TGCCCAGGGC TCACACGTGT
    TTCAACCGGC TGGATCTTCC CCCCTATCCT TCATTCTCCA TGCTCTACGA GAAGATGCTG
    ACGGCAGTGG AGGAGACCAG CACTTTCGGT TTGGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.