HECW2 cDNA ORF clone, Canis lupus familiaris(Dog)

The following HECW2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe284810 XM_022414999.1
Latest version!
Canis lupus familiaris HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00
OCe45143 XM_014110904.1
Latest version!
Canis lupus familiaris HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe284810
    Clone ID Related Accession (Same CDS sequence) XM_022414999.1
    Accession Version XM_022414999.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4692bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product E3 ubiquitin-protein ligase HECW2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006619.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCCAGCT CCGCCCGGGA GCACCTGCTG TTCGTGCGGC GCCGGAACCC CCAGATGCGG 
    TACACGCTGA GCCCCGAGAA CCTCCAGAGC CTCGCGGCCC AGAGCTCTAT GCCGGAGAAC
    ATGACCCTCC CGAGGGCGAA CAGTGACACG GACCTGGTGA CCTCTGAGAG CCGCTCCAGC
    TTGACCGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGGC TGTACCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACTCA AAAAGGGCAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT
    AAATACTACC ACGGCATTAG TGGAGCCCTT CGCGCCACGA CCCCCTGCAT TACGGTGAAG
    AACCCCGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTTCTGG AAATCAGCAT
    TCTCGGAAGC TAGTTAGCTT TACGTTGTCA GATCTTAGAG CAGTTGGGCT AAAGAAAGGT
    ATGTTCTTTA ATCCTGATCC TTATCTGAAG ATGTCCATCC AGCCAGGGAA GAAGAGCAGC
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGAAGGTCTA CTATCATCAG TAACACCACT
    AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATTC CAGTCCAGAG GTTGCTGGAG CGGCAAGCCA TTGGGGACCA AATGCTCAGC
    TACAACCTTG GCCGGAGGCT CCCAGCTGAC CACGTGAGCG GCTACCTCCA GTTTAAAGTG
    GAGGTCACGT CTTCCGTGCA CGAAGACGCT TCTCCGGAAG CCGTGGGCAC AATCCTTGGG
    GTCAGTACGG TGAACGGAGA CCTGGGAAGC CCTTCTGATG AGGAGGATGT GCCAGGGGGC
    CACCAGGACA GCCCCGCTTG CTCCAACGGC CCGGTTTCGG AGGACAGCGC TGCAGACGCG
    ACGCCCAAGC GCGCTCTCAG GACTAGCTCC ACGCTCGACA TGGACTCCGA GGACCTGAGC
    TCCGCCGCGT CCAGGGCCTC GCCCCCCCGG GGCCGCCAGG ATTCGCTCAA CGACTACCTG
    GACGCCATCG AACGCGCGGC CGCCTGCCCC GACAGGTCCC TGGGCGCCTC CCCCAAACTC
    AGGAGTAGTT TTCCCACCGA CACGAGGCTC AACGCGATGC TGCACATCGA CTCCGACGAG
    GAGGAGCACG AGTTCCAGCA AGGCCTGGGG TACCCGGGCT CCCCCGAGGA GGACGAGGAG
    GGCGGGGGGC TGGTCATGGC GGGCAGCGCA CCCGCGGGCC GGACCCCCCA GGCCGGGCCG
    CAGGGCAGCC CCGCGGACCG GGCCGGGGCC TTGCACGCCG AGGACAAGCC GGGCCGCGCC
    CCCGAGCCCG CCGCCGCCGA GGGCGCCCCG GACGCCGAGC AGGTGGCCCG CGGGGCGGAG
    CCGGGCTCGC CCGACGCCGA GGCCTTGGAG CATGAGCCGG AGCAGGAGCC GGAGCAGGAG
    CAGGAGCCGG CGCCCGAGCA GGAGCAGGAG CAGGAGCAGG AGCAGGACCA GGCCTCCTCC
    GAGACGGAGC CCAGCGACCC CGCCCCGACC GAGAGCGCGA GCGCCAGCGA GGCCAGCACG
    CGGCCCGAGG GCGAGAGCGA CCTGGAGGGC GCCGACGGGC CCGGGCCCTG CGCCCACACG
    CGGGGCCCCG CGCCCGAGGG GGAGGCGGCG GCGGCGGCGG CGGAGGAGCA GGAGCAGCAG
    CAGGAGCAGG AGCAGGAGGA GCCGGAGGAG CCGGAGGAGG CGGGCGGCGA GGAGCAGCGC
    GGTGCCTGCG GAGGCCCCGC CGCGCCCCGG GAGCCCAACG CCCTGCCCGC CCTGCCCGCC
    GTGCAGGTGC CCCGCGGGGC CGGGGCCGGG GCCGGGGCCG GGGATGCGCC CGGGGCGCAC
    GACGTGTGGC GGCGCAGGGG CAGCCTGCAG GGGGCGGCCG AGCGCCCGCG GGAGGAGCAG
    GAGCAGGAGC AGGACGAGCG GGAGCGGGAG CGGGAGCAGC CGCGGGAGGA GCGGGAGCCC
    GAGCCCGAGC CCGAGCCGGA CGCCGACGCC CCGGCCGCCC GCGGAGCCTG CGAGGGGGCC
    GCTGCGCAGG AGGAGGGCGC CGCCGCCGGA GATTCTCAAG CCAATGGCCA CCAACCATTG
    AGATCGTTGC CTTCGGTGCG CCAGGACGTT AGCCGCTACC AGAGGGTGGA CGAGGCTCTC
    CCACCAAACT GGGAGGCGCG CATTGACAGC CACGGCAGGA TCTTCTACGT GGATCACGTG
    AACAGAACCA CCACATGGCA GCGGCCAACA GCGCCTCCAG CCCCCCAGGT GCTGCAGAGA
    TCAAACTCCA TACAGCAAAT GGAGCAGCTG AACCGGAGAT ACCAGAGTAT CCGCAGAACC
    ATGACTAATG AGAGGCCTGA GGAAAATACC AATGCGATCG ATGGGGCAGG GGAGGAAGCT
    GACTTTCACC AAGCCAGTGC AGATTTCCGA CGGGAAAACG TCCTGCCTCA CTCTACCTCC
    AGATCCAGGC TCACGTTGCT GTTACAGTCA CCCCCTGTGA AGTTCCTCAT CAGCCCAGAC
    TTCTTCACCG TGCTGCATTC TAACCCTAGT GCCTACCGCA TGTTTACAAA CAATACGTGT
    TTGAAGCACA TGATCACCAA AGTCCGGCGG GACACCCACC ACTTTGAACG CTACCAGCAT
    AACCGAGATC TTGTGGGATT CCTCAATATG TTCGCCAACA AACAGCTGGA GCTGCCAAGG
    GGCTGGGAGA TGAAACACGA TCATCAGGGC AAGGCATTTT TTGTCGACCA CAACTCCCGC
    ACCACCACTT TTATCGACCC CCGGCTCCCA CTCCAGAGCA GTAGACCCAC GAGCGCGCTG
    GTTCATCGGC AGCACCTGAC CAGGCAACGC AGCCACAGCG CAGGCGAGGT AGGAGAAGAT
    TCTCGACATA CAGGACCACC AGTTCTTCCC AGGCCATCAA GTACATTCAA TACAGTCAGT
    AGGCCACAGT ACCAGGATAT GGTTCCAGTG GCTTACAATG ACAAGATTGT TGCATTTCTG
    CGCCAACCCA ACATCTTTGA AATTCTGCAG GAGCGTCAAC CTGATCTTAC CAGGAATCAT
    TCACTCAGGG AGAAGATCCA ATTTATCCGA ACTGAAGGGA CCCCAGGATT GGTGCGTCTT
    TCAAGTGATG CAGACCTTGT TATGTTGCTG AGTTTATTTG AAGAAGAGAT AATGTCTTAT
    GTGCCTCCAC ATGCCTTACT TCACCCCAGC TACTGTCAGT CTCCACGTGG TTCCCCTGTG
    TCCTCTCCCC AGAACTCGCC GGGTACTCAG CGTGCCAATG CCCGGGCTCC AGCCCCTTAC
    AAGCGGGACT TTGAAGCCAA ACTAAGGAAC TTTTACAGGA AGTTAGAGAC TAAAGGATAC
    GGACAAGGCC CAGGGAAATT AAAGTTAATT ATCAGAAGAG ATCACTTACT AGAAGATGCT
    TTCAATCAGA TTATGGGCTA CTCCAGAAAA GACTTGCAGA GAAATAAGCT GTACGTCACG
    TTCGTCGGGG AGGAAGGGCT GGATTACAGT GGACCTTCCA GAGAGTTTTT CTTCCTCGTA
    TCCAGAGAAC TCTTTAACCC ATATTATGGC TTATTTGAAT ATTCGGCCAA TGACACATAC
    ACAGTACAGA TAAGTCCCAT GTCTGCTTTT GTAGACAATC ACCATGAATG GTTCCGGTTC
    AGTGGTAGGA TCCTTGGTCT CGCACTAATA CACCAGTATT TGTTGGATGC CTTCTTCACA
    CGGCCCTTTT ATAAAGCCCT TCTCAGAATT CTGTGTGACC TGAGTGATCT AGAATACCTT
    GACGAAGAGT TCCACCAGAG CCTGCAGTGG ATGAAAGACA ATGACATCCA TGACATCTTG
    GACCTTACAT TCACTGTGAA TGAAGAAGTT TTTGGGCAGA TTACCGAACG AGAATTAAAA
    CCAGGGGGTG CCAATATCCC AGTCACAGAG AAGAACAAGA AGGAGTACAT CGAAAGGATG
    GTGAAATGGA GGATTGAGAG GGGCGTCGTA CAGCAAACGG AGAGCTTAGT GCGTGGCTTC
    TATGAGGTGG TGGATGCCAG GCTGGTGTCT GTTTTTGATG CAAGAGAACT GGAACTGGTC
    ATCGCAGGCA CAGCTGAAAT CGACCTGAGT GACTGGAGAA ACAACACAGA ATATAGAGGA
    GGATATCATG ACAATCATAT TGTAATTCGG TGGTTCTGGG CTGCAGTGGA AAGATTCAAC
    AATGAACAAC GACTAAGGTT GTTACAGTTT GTCACGGGCA CATCCAGCAT TCCCTATGAA
    GGATTTGCTT CTCTCCGAGG GAGTAATGGC CCAAGAAGAT TCTGCGTGGA GAAATGGGGG
    AAAATCACTG CTCTTCCCAG AGCTCACACT TGTTTTAACC GTCTGGATCT CCCCCCATAC
    CCATCCTTCT CCATGCTTTA TGAAAAACTC TTGACAGCAG TTGAAGAGAC CAGCACCTTT
    GGACTTGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022270707.1
    CDS154..4845
    Translation

    Target ORF information:

    RefSeq Version XM_022414999.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022414999.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCCAGCT CCGCCCGGGA GCACCTGCTG TTCGTGCGGC GCCGGAACCC CCAGATGCGG 
    TACACGCTGA GCCCCGAGAA CCTCCAGAGC CTCGCGGCCC AGAGCTCTAT GCCGGAGAAC
    ATGACCCTCC CGAGGGCGAA CAGTGACACG GACCTGGTGA CCTCTGAGAG CCGCTCCAGC
    TTGACCGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGGC TGTACCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACTCA AAAAGGGCAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT
    AAATACTACC ACGGCATTAG TGGAGCCCTT CGCGCCACGA CCCCCTGCAT TACGGTGAAG
    AACCCCGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTTCTGG AAATCAGCAT
    TCTCGGAAGC TAGTTAGCTT TACGTTGTCA GATCTTAGAG CAGTTGGGCT AAAGAAAGGT
    ATGTTCTTTA ATCCTGATCC TTATCTGAAG ATGTCCATCC AGCCAGGGAA GAAGAGCAGC
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGAAGGTCTA CTATCATCAG TAACACCACT
    AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATTC CAGTCCAGAG GTTGCTGGAG CGGCAAGCCA TTGGGGACCA AATGCTCAGC
    TACAACCTTG GCCGGAGGCT CCCAGCTGAC CACGTGAGCG GCTACCTCCA GTTTAAAGTG
    GAGGTCACGT CTTCCGTGCA CGAAGACGCT TCTCCGGAAG CCGTGGGCAC AATCCTTGGG
    GTCAGTACGG TGAACGGAGA CCTGGGAAGC CCTTCTGATG AGGAGGATGT GCCAGGGGGC
    CACCAGGACA GCCCCGCTTG CTCCAACGGC CCGGTTTCGG AGGACAGCGC TGCAGACGCG
    ACGCCCAAGC GCGCTCTCAG GACTAGCTCC ACGCTCGACA TGGACTCCGA GGACCTGAGC
    TCCGCCGCGT CCAGGGCCTC GCCCCCCCGG GGCCGCCAGG ATTCGCTCAA CGACTACCTG
    GACGCCATCG AACGCGCGGC CGCCTGCCCC GACAGGTCCC TGGGCGCCTC CCCCAAACTC
    AGGAGTAGTT TTCCCACCGA CACGAGGCTC AACGCGATGC TGCACATCGA CTCCGACGAG
    GAGGAGCACG AGTTCCAGCA AGGCCTGGGG TACCCGGGCT CCCCCGAGGA GGACGAGGAG
    GGCGGGGGGC TGGTCATGGC GGGCAGCGCA CCCGCGGGCC GGACCCCCCA GGCCGGGCCG
    CAGGGCAGCC CCGCGGACCG GGCCGGGGCC TTGCACGCCG AGGACAAGCC GGGCCGCGCC
    CCCGAGCCCG CCGCCGCCGA GGGCGCCCCG GACGCCGAGC AGGTGGCCCG CGGGGCGGAG
    CCGGGCTCGC CCGACGCCGA GGCCTTGGAG CATGAGCCGG AGCAGGAGCC GGAGCAGGAG
    CAGGAGCCGG CGCCCGAGCA GGAGCAGGAG CAGGAGCAGG AGCAGGACCA GGCCTCCTCC
    GAGACGGAGC CCAGCGACCC CGCCCCGACC GAGAGCGCGA GCGCCAGCGA GGCCAGCACG
    CGGCCCGAGG GCGAGAGCGA CCTGGAGGGC GCCGACGGGC CCGGGCCCTG CGCCCACACG
    CGGGGCCCCG CGCCCGAGGG GGAGGCGGCG GCGGCGGCGG CGGAGGAGCA GGAGCAGCAG
    CAGGAGCAGG AGCAGGAGGA GCCGGAGGAG CCGGAGGAGG CGGGCGGCGA GGAGCAGCGC
    GGTGCCTGCG GAGGCCCCGC CGCGCCCCGG GAGCCCAACG CCCTGCCCGC CCTGCCCGCC
    GTGCAGGTGC CCCGCGGGGC CGGGGCCGGG GCCGGGGCCG GGGATGCGCC CGGGGCGCAC
    GACGTGTGGC GGCGCAGGGG CAGCCTGCAG GGGGCGGCCG AGCGCCCGCG GGAGGAGCAG
    GAGCAGGAGC AGGACGAGCG GGAGCGGGAG CGGGAGCAGC CGCGGGAGGA GCGGGAGCCC
    GAGCCCGAGC CCGAGCCGGA CGCCGACGCC CCGGCCGCCC GCGGAGCCTG CGAGGGGGCC
    GCTGCGCAGG AGGAGGGCGC CGCCGCCGGA GATTCTCAAG CCAATGGCCA CCAACCATTG
    AGATCGTTGC CTTCGGTGCG CCAGGACGTT AGCCGCTACC AGAGGGTGGA CGAGGCTCTC
    CCACCAAACT GGGAGGCGCG CATTGACAGC CACGGCAGGA TCTTCTACGT GGATCACGTG
    AACAGAACCA CCACATGGCA GCGGCCAACA GCGCCTCCAG CCCCCCAGGT GCTGCAGAGA
    TCAAACTCCA TACAGCAAAT GGAGCAGCTG AACCGGAGAT ACCAGAGTAT CCGCAGAACC
    ATGACTAATG AGAGGCCTGA GGAAAATACC AATGCGATCG ATGGGGCAGG GGAGGAAGCT
    GACTTTCACC AAGCCAGTGC AGATTTCCGA CGGGAAAACG TCCTGCCTCA CTCTACCTCC
    AGATCCAGGC TCACGTTGCT GTTACAGTCA CCCCCTGTGA AGTTCCTCAT CAGCCCAGAC
    TTCTTCACCG TGCTGCATTC TAACCCTAGT GCCTACCGCA TGTTTACAAA CAATACGTGT
    TTGAAGCACA TGATCACCAA AGTCCGGCGG GACACCCACC ACTTTGAACG CTACCAGCAT
    AACCGAGATC TTGTGGGATT CCTCAATATG TTCGCCAACA AACAGCTGGA GCTGCCAAGG
    GGCTGGGAGA TGAAACACGA TCATCAGGGC AAGGCATTTT TTGTCGACCA CAACTCCCGC
    ACCACCACTT TTATCGACCC CCGGCTCCCA CTCCAGAGCA GTAGACCCAC GAGCGCGCTG
    GTTCATCGGC AGCACCTGAC CAGGCAACGC AGCCACAGCG CAGGCGAGGT AGGAGAAGAT
    TCTCGACATA CAGGACCACC AGTTCTTCCC AGGCCATCAA GTACATTCAA TACAGTCAGT
    AGGCCACAGT ACCAGGATAT GGTTCCAGTG GCTTACAATG ACAAGATTGT TGCATTTCTG
    CGCCAACCCA ACATCTTTGA AATTCTGCAG GAGCGTCAAC CTGATCTTAC CAGGAATCAT
    TCACTCAGGG AGAAGATCCA ATTTATCCGA ACTGAAGGGA CCCCAGGATT GGTGCGTCTT
    TCAAGTGATG CAGACCTTGT TATGTTGCTG AGTTTATTTG AAGAAGAGAT AATGTCTTAT
    GTGCCTCCAC ATGCCTTACT TCACCCCAGC TACTGTCAGT CTCCACGTGG TTCCCCTGTG
    TCCTCTCCCC AGAACTCGCC GGGTACTCAG CGTGCCAATG CCCGGGCTCC AGCCCCTTAC
    AAGCGGGACT TTGAAGCCAA ACTAAGGAAC TTTTACAGGA AGTTAGAGAC TAAAGGATAC
    GGACAAGGCC CAGGGAAATT AAAGTTAATT ATCAGAAGAG ATCACTTACT AGAAGATGCT
    TTCAATCAGA TTATGGGCTA CTCCAGAAAA GACTTGCAGA GAAATAAGCT GTACGTCACG
    TTCGTCGGGG AGGAAGGGCT GGATTACAGT GGACCTTCCA GAGAGTTTTT CTTCCTCGTA
    TCCAGAGAAC TCTTTAACCC ATATTATGGC TTATTTGAAT ATTCGGCCAA TGACACATAC
    ACAGTACAGA TAAGTCCCAT GTCTGCTTTT GTAGACAATC ACCATGAATG GTTCCGGTTC
    AGTGGTAGGA TCCTTGGTCT CGCACTAATA CACCAGTATT TGTTGGATGC CTTCTTCACA
    CGGCCCTTTT ATAAAGCCCT TCTCAGAATT CTGTGTGACC TGAGTGATCT AGAATACCTT
    GACGAAGAGT TCCACCAGAG CCTGCAGTGG ATGAAAGACA ATGACATCCA TGACATCTTG
    GACCTTACAT TCACTGTGAA TGAAGAAGTT TTTGGGCAGA TTACCGAACG AGAATTAAAA
    CCAGGGGGTG CCAATATCCC AGTCACAGAG AAGAACAAGA AGGAGTACAT CGAAAGGATG
    GTGAAATGGA GGATTGAGAG GGGCGTCGTA CAGCAAACGG AGAGCTTAGT GCGTGGCTTC
    TATGAGGTGG TGGATGCCAG GCTGGTGTCT GTTTTTGATG CAAGAGAACT GGAACTGGTC
    ATCGCAGGCA CAGCTGAAAT CGACCTGAGT GACTGGAGAA ACAACACAGA ATATAGAGGA
    GGATATCATG ACAATCATAT TGTAATTCGG TGGTTCTGGG CTGCAGTGGA AAGATTCAAC
    AATGAACAAC GACTAAGGTT GTTACAGTTT GTCACGGGCA CATCCAGCAT TCCCTATGAA
    GGATTTGCTT CTCTCCGAGG GAGTAATGGC CCAAGAAGAT TCTGCGTGGA GAAATGGGGG
    AAAATCACTG CTCTTCCCAG AGCTCACACT TGTTTTAACC GTCTGGATCT CCCCCCATAC
    CCATCCTTCT CCATGCTTTA TGAAAAACTC TTGACAGCAG TTGAAGAGAC CAGCACCTTT
    GGACTTGAGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe45143
    Clone ID Related Accession (Same CDS sequence) XM_014110904.1
    Accession Version XM_014110904.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4611bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product E3 ubiquitin-protein ligase HECW2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726123.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 4% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCCAGCT CCGCCCGGGA GCACCTGCTG TTCGTGCGGC GCCGGAACCC CCAGATGCGG 
    TACACGCTGA GCCCCGAGAA CCTCCAGAGC CTCGCGGCCC AGAGCTCTAT GCCGGAGAAC
    ATGACCCTCC CGAGGGCGAA CAGTGACACG GACCTGGTGA CCTCTGAGAG CCGCTCCAGC
    TTGACCGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGGC TGTACCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACTCA AAAAGGGCAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT
    AAATACTACC ACGGCATTAG TGGAGCCCTT CGCGCCACGA CCCCCTGCAT TACGGTGAAG
    AACCCCGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTTCTGG AAATCAGCAT
    TCTCGGAAGC TAGTTAGCTT TACGTTGTCA GATCTTAGAG CAGTTGGGCT AAAGAAAGGT
    ATGTTCTTTA ATCCTGATCC TTATCTGAAG ATGTCCATCC AGCCAGGGAA GAAGAGCAGC
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGAAGGTCTA CTATCATCAG TAACACCACT
    AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATTC CAGTCCAGAG GTTGCTGGAG CGGCAAGCCA TTGGGGACCA AATGCTCAGC
    TACAACCTTG GCCGGAGGCT CCCAGCTGAC CACGTGAGCG GCTACCTCCA GTTTAAAGTG
    GAGGTCACGT CTTCCGTGCA CGAAGACGCT TCTCCGGAAG CCGTGGGCAC AATCCTTGGG
    GTCAGTACGG TGAACGGAGA CCTGGGAAGC CCTTCTGATG AGGAGGATGT GCCAGGGGGC
    CACCAGGACA GCCCCGCTTG CTCCAACGGC CCGGTTTCGG AGGACAGCGC TGCAGACGCG
    ACGCCCAAGC GCGCTCTCAG GACTAGCTCC ACGCTCGACA TGGACTCCGA GGACCTGAGC
    TCCGCCGCGT CCAGGGCCTC GCCCCCCCGG GGCCGCCAGG ATTCGCTCAA CGACTACCTG
    GACGCCATCG AACGCGCGGC CGCCTGCCCC GACAGGTCCC TGGGCGCCTC CCCCAAACTC
    AGGAGTAGTT TTCCCACCGA CACGAGGCTC AACGCGATGC TGCACATCGA CTCCGACGAG
    GAGGAGCACG AGTTCCAGCA AGGCCTGGGG TACCCGGGCT CCCCCGAGGA GGACGAGGAG
    GGCGGGGGGC TGGTCATGGC GGGCAGCGCA CCCGCGGGCC GGACCCCCCA GGCCGGGCCG
    CAGGGCAGCC CCGCGGACCG GGCCGGGGCC TTGCACGCCG AGGACAAGCC GGGCCGCGCC
    CCCGAGCCCG CCGCCGCCGA GGGCGCCCCG GACGCCGAGC AGGTGGCCCG CGGGGCGGAG
    CCGGGCTCGC CCGACGCCGA GGCCTTGGAG CATGAGCCGG AGCAGGAGCC GGAGCAGGAG
    CAGGAGCCGG CGCCCGAGCA GGAGCAGGAG CAGGAGCAGG AGCAGGACCA GGCCTCCTCC
    GAGACGGAGC CCAGCGACCC CGCCCCGACC GAGAGCGCGA GCGCCAGCGA GGCCAGCACG
    CGGCCCGAGG GCGAGAGCGA CCTGGAGGGC GCCGACGGGC CCGGGCCCTG CGCCCACACG
    CGGGGCCCCG CGCCCGAGGG GGAGGCGGCG GCGGCGGCGG CGGAGGAGCA GGAGCAGCAG
    CAGGAGCAGG AGCAGGAGGA GCCGGAGGAG CCGGAGGAGG CGGGCGGCGA GGAGCAGCGC
    GGTGCCTGCG GAGGCCCCGC CGCGCCCCGG GAGCCCAACG CCCTGCCCGC CCTGCCCGCC
    GTGCAGGTGC CCCGCGGGGC CGGGGCCGGG GCCGGGGCCG GGGATGCGCC CGGGGCGCAC
    GACCCGCGGG AGGAGCGGGA GCCCGAGCCC GAGCCCGAGC CGGACGCCGA CGCCCCGGCC
    GCCCGCGGAG CCTGCGAGGG GGCCGCTGCG CAGGAGGAGG GCGCCGCCGC CGGAGGTAGG
    ATGCTCGCAG ATTCTCAAGC CAATGGCCAC CAACCATTGA GATCGTTGCC TTCGGTGCGC
    CAGGACGTTA GCCGCTACCA GAGGGTGGAC GAGGCTCTCC CACCAAACTG GGAGGCGCGC
    ATTGACAGCC ACGGCAGGAT CTTCTACGTG GATCACGTGA ACAGAACCAC CACATGGCAG
    CGGCCAACAG CGCCTCCAGC CCCCCAGGTG CTGCAGAGAT CAAACTCCAT ACAGCAAATG
    GAGCAGCTGA ACCGGAGATA CCAGAGTATC CGCAGAACCA TGACTAATGA GAGGCCTGAG
    GAAAATACCA ATGCGATCGA TGGGGCAGGG GAGGAAGCTG ACTTTCACCA AGCCAGTGCA
    GATTTCCGAC GGGAAAACGT CCTGCCTCAC TCTACCTCCA GATCCAGGCT CACGTTGCTG
    TTACAGTCAC CCCCTGTGAA GTTCCTCATC AGCCCAGACT TCTTCACCGT GCTGCATTCT
    AACCCTAGTG CCTACCGCAT GTTTACAAAC AATACGTGTT TGAAGCACAT GATCACCAAA
    GTCCGGCGGG ACACCCACCA CTTTGAACGC TACCAGCATA ACCGAGATCT TGTGGGATTC
    CTCAATATGT TCGCCAACAA ACAGCTGGAG CTGCCAAGGG GCTGGGAGAT GAAACACGAT
    CATCAGGGCA AGGCATTTTT TGTCGACCAC AACTCCCGCA CCACCACTTT TATCGACCCC
    CGGCTCCCAC TCCAGAGCAG TAGACCCACG AGCGCGCTGG TTCATCGGCA GCACCTGACC
    AGGCAACGCA GCCACAGCGC AGGCGAGGTA GGAGAAGATT CTCGACATAC AGGACCACCA
    GTTCTTCCCA GGCCATCAAG TACATTCAAT ACAGTCAGTA GGCCACAGTA CCAGGATATG
    GTTCCAGTGG CTTACAATGA CAAGATTGTT GCATTTCTGC GCCAACCCAA CATCTTTGAA
    ATTCTGCAGG AGCGTCAACC TGATCTTACC AGGAATCATT CACTCAGGGA GAAGATCCAA
    TTTATCCGAA CTGAAGGGAC CCCAGGATTG GTGCGTCTTT CAAGTGATGC AGACCTTGTT
    ATGTTGCTGA GTTTATTTGA AGAAGAGATA ATGTCTTATG TGCCTCCACA TGCCTTACTT
    CACCCCAGCT ACTGTCAGTC TCCACGTGGT TCCCCTGTGT CCTCTCCCCA GAACTCGCCG
    GGTACTCAGC GTGCCAATGC CCGGGCTCCA GCCCCTTACA AGCGGGACTT TGAAGCCAAA
    CTAAGGAACT TTTACAGGAA GTTAGAGACT AAAGGATACG GACAAGGCCC AGGGAAATTA
    AAGTTAATTA TCAGAAGAGA TCACTTACTA GAAGATGCTT TCAATCAGAT TATGGGCTAC
    TCCAGAAAAG ACTTGCAGAG AAATAAGCTG TACGTCACGT TCGTCGGGGA GGAAGGGCTG
    GATTACAGTG GACCTTCCAG AGAGTTTTTC TTCCTCGTAT CCAGAGAACT CTTTAACCCA
    TATTATGGCT TATTTGAATA TTCGGCCAAT GACACATACA CAGTACAGAT AAGTCCCATG
    TCTGCTTTTG TAGACAATCA CCATGAATGG TTCCGGTTCA GTGGTAGGAT CCTTGGTCTC
    GCACTAATAC ACCAGTATTT GTTGGATGCC TTCTTCACAC GGCCCTTTTA TAAAGCCCTT
    CTCAGAATTC TGTGTGACCT GAGTGATCTA GAATACCTTG ACGAAGAGTT CCACCAGAGC
    CTGCAGTGGA TGAAAGACAA TGACATCCAT GACATCTTGG ACCTTACATT CACTGTGAAT
    GAAGAAGTTT TTGGGCAGAT TACCGAACGA GAATTAAAAC CAGGGGGTGC CAATATCCCA
    GTCACAGAGA AGAACAAGAA GGAGTACATC GAAAGGATGG TGAAATGGAG GATTGAGAGG
    GGCGTCGTAC AGCAAACGGA GAGCTTAGTG CGTGGCTTCT ATGAGGTGGT GGATGCCAGG
    CTGGTGTCTG TTTTTGATGC AAGAGAACTG GAACTGGTCA TCGCAGGCAC AGCTGAAATC
    GACCTGAGTG ACTGGAGAAA CAACACAGAA TATAGAGGAG GATATCATGA CAATCATATT
    GTAATTCGGT GGTTCTGGGC TGCAGTGGAA AGATTCAACA ATGAACAACG ACTAAGGTTG
    TTACAGTTTG TCACGGGCAC ATCCAGCATT CCCTATGAAG GATTTGCTTC TCTCCGAGGG
    AGTAATGGCC CAAGAAGATT CTGCGTGGAG AAATGGGGGA AAATCACTGC TCTTCCCAGA
    GCTCACACTT GTTTTAACCG TCTGGATCTC CCCCCATACC CATCCTTCTC CATGCTTTAT
    GAAAAACTCT TGACAGCAGT TGAAGAGACC AGCACCTTTG GACTTGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013966379.1
    CDS447..5057
    Translation

    Target ORF information:

    RefSeq Version XM_014110904.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014110904.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGCCAGCT CCGCCCGGGA GCACCTGCTG TTCGTGCGGC GCCGGAACCC CCAGATGCGG 
    TACACGCTGA GCCCCGAGAA CCTCCAGAGC CTCGCGGCCC AGAGCTCTAT GCCGGAGAAC
    ATGACCCTCC CGAGGGCGAA CAGTGACACG GACCTGGTGA CCTCTGAGAG CCGCTCCAGC
    TTGACCGCCA GCATGTACGA GTACACGCTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAAG AGGAGGTGGA CCCCAGTGAT TGGATTGGGC TGTACCACAT AGATGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAGAAC AGGGGTGTGA CTGGAACTCA AAAAGGGCAA
    ATTGTATGGA GAATTGAGCC TGGGCCCTAT TTCATGGAAC CGGAGATAAA AATCTGTTTT
    AAATACTACC ACGGCATTAG TGGAGCCCTT CGCGCCACGA CCCCCTGCAT TACGGTGAAG
    AACCCCGCTG TGATGATGGG GGCAGAAGGC ATGGAAGGAG GTGCTTCTGG AAATCAGCAT
    TCTCGGAAGC TAGTTAGCTT TACGTTGTCA GATCTTAGAG CAGTTGGGCT AAAGAAAGGT
    ATGTTCTTTA ATCCTGATCC TTATCTGAAG ATGTCCATCC AGCCAGGGAA GAAGAGCAGC
    TTCCCCACCT GTGCCCACCA TGGGCAGGAG AGAAGGTCTA CTATCATCAG TAACACCACT
    AATCCAATTT GGCACCGAGA GAAATATTCC TTTTTTGCAC TTCTTACTGA TGTCTTAGAA
    ATTGAAATTA AAGACAAATT TGCCAAGAGT CGTCCCATCA TCAAGCGTTT TCTGGGGAAA
    CTAACCATTC CAGTCCAGAG GTTGCTGGAG CGGCAAGCCA TTGGGGACCA AATGCTCAGC
    TACAACCTTG GCCGGAGGCT CCCAGCTGAC CACGTGAGCG GCTACCTCCA GTTTAAAGTG
    GAGGTCACGT CTTCCGTGCA CGAAGACGCT TCTCCGGAAG CCGTGGGCAC AATCCTTGGG
    GTCAGTACGG TGAACGGAGA CCTGGGAAGC CCTTCTGATG AGGAGGATGT GCCAGGGGGC
    CACCAGGACA GCCCCGCTTG CTCCAACGGC CCGGTTTCGG AGGACAGCGC TGCAGACGCG
    ACGCCCAAGC GCGCTCTCAG GACTAGCTCC ACGCTCGACA TGGACTCCGA GGACCTGAGC
    TCCGCCGCGT CCAGGGCCTC GCCCCCCCGG GGCCGCCAGG ATTCGCTCAA CGACTACCTG
    GACGCCATCG AACGCGCGGC CGCCTGCCCC GACAGGTCCC TGGGCGCCTC CCCCAAACTC
    AGGAGTAGTT TTCCCACCGA CACGAGGCTC AACGCGATGC TGCACATCGA CTCCGACGAG
    GAGGAGCACG AGTTCCAGCA AGGCCTGGGG TACCCGGGCT CCCCCGAGGA GGACGAGGAG
    GGCGGGGGGC TGGTCATGGC GGGCAGCGCA CCCGCGGGCC GGACCCCCCA GGCCGGGCCG
    CAGGGCAGCC CCGCGGACCG GGCCGGGGCC TTGCACGCCG AGGACAAGCC GGGCCGCGCC
    CCCGAGCCCG CCGCCGCCGA GGGCGCCCCG GACGCCGAGC AGGTGGCCCG CGGGGCGGAG
    CCGGGCTCGC CCGACGCCGA GGCCTTGGAG CATGAGCCGG AGCAGGAGCC GGAGCAGGAG
    CAGGAGCCGG CGCCCGAGCA GGAGCAGGAG CAGGAGCAGG AGCAGGACCA GGCCTCCTCC
    GAGACGGAGC CCAGCGACCC CGCCCCGACC GAGAGCGCGA GCGCCAGCGA GGCCAGCACG
    CGGCCCGAGG GCGAGAGCGA CCTGGAGGGC GCCGACGGGC CCGGGCCCTG CGCCCACACG
    CGGGGCCCCG CGCCCGAGGG GGAGGCGGCG GCGGCGGCGG CGGAGGAGCA GGAGCAGCAG
    CAGGAGCAGG AGCAGGAGGA GCCGGAGGAG CCGGAGGAGG CGGGCGGCGA GGAGCAGCGC
    GGTGCCTGCG GAGGCCCCGC CGCGCCCCGG GAGCCCAACG CCCTGCCCGC CCTGCCCGCC
    GTGCAGGTGC CCCGCGGGGC CGGGGCCGGG GCCGGGGCCG GGGATGCGCC CGGGGCGCAC
    GACCCGCGGG AGGAGCGGGA GCCCGAGCCC GAGCCCGAGC CGGACGCCGA CGCCCCGGCC
    GCCCGCGGAG CCTGCGAGGG GGCCGCTGCG CAGGAGGAGG GCGCCGCCGC CGGAGGTAGG
    ATGCTCGCAG ATTCTCAAGC CAATGGCCAC CAACCATTGA GATCGTTGCC TTCGGTGCGC
    CAGGACGTTA GCCGCTACCA GAGGGTGGAC GAGGCTCTCC CACCAAACTG GGAGGCGCGC
    ATTGACAGCC ACGGCAGGAT CTTCTACGTG GATCACGTGA ACAGAACCAC CACATGGCAG
    CGGCCAACAG CGCCTCCAGC CCCCCAGGTG CTGCAGAGAT CAAACTCCAT ACAGCAAATG
    GAGCAGCTGA ACCGGAGATA CCAGAGTATC CGCAGAACCA TGACTAATGA GAGGCCTGAG
    GAAAATACCA ATGCGATCGA TGGGGCAGGG GAGGAAGCTG ACTTTCACCA AGCCAGTGCA
    GATTTCCGAC GGGAAAACGT CCTGCCTCAC TCTACCTCCA GATCCAGGCT CACGTTGCTG
    TTACAGTCAC CCCCTGTGAA GTTCCTCATC AGCCCAGACT TCTTCACCGT GCTGCATTCT
    AACCCTAGTG CCTACCGCAT GTTTACAAAC AATACGTGTT TGAAGCACAT GATCACCAAA
    GTCCGGCGGG ACACCCACCA CTTTGAACGC TACCAGCATA ACCGAGATCT TGTGGGATTC
    CTCAATATGT TCGCCAACAA ACAGCTGGAG CTGCCAAGGG GCTGGGAGAT GAAACACGAT
    CATCAGGGCA AGGCATTTTT TGTCGACCAC AACTCCCGCA CCACCACTTT TATCGACCCC
    CGGCTCCCAC TCCAGAGCAG TAGACCCACG AGCGCGCTGG TTCATCGGCA GCACCTGACC
    AGGCAACGCA GCCACAGCGC AGGCGAGGTA GGAGAAGATT CTCGACATAC AGGACCACCA
    GTTCTTCCCA GGCCATCAAG TACATTCAAT ACAGTCAGTA GGCCACAGTA CCAGGATATG
    GTTCCAGTGG CTTACAATGA CAAGATTGTT GCATTTCTGC GCCAACCCAA CATCTTTGAA
    ATTCTGCAGG AGCGTCAACC TGATCTTACC AGGAATCATT CACTCAGGGA GAAGATCCAA
    TTTATCCGAA CTGAAGGGAC CCCAGGATTG GTGCGTCTTT CAAGTGATGC AGACCTTGTT
    ATGTTGCTGA GTTTATTTGA AGAAGAGATA ATGTCTTATG TGCCTCCACA TGCCTTACTT
    CACCCCAGCT ACTGTCAGTC TCCACGTGGT TCCCCTGTGT CCTCTCCCCA GAACTCGCCG
    GGTACTCAGC GTGCCAATGC CCGGGCTCCA GCCCCTTACA AGCGGGACTT TGAAGCCAAA
    CTAAGGAACT TTTACAGGAA GTTAGAGACT AAAGGATACG GACAAGGCCC AGGGAAATTA
    AAGTTAATTA TCAGAAGAGA TCACTTACTA GAAGATGCTT TCAATCAGAT TATGGGCTAC
    TCCAGAAAAG ACTTGCAGAG AAATAAGCTG TACGTCACGT TCGTCGGGGA GGAAGGGCTG
    GATTACAGTG GACCTTCCAG AGAGTTTTTC TTCCTCGTAT CCAGAGAACT CTTTAACCCA
    TATTATGGCT TATTTGAATA TTCGGCCAAT GACACATACA CAGTACAGAT AAGTCCCATG
    TCTGCTTTTG TAGACAATCA CCATGAATGG TTCCGGTTCA GTGGTAGGAT CCTTGGTCTC
    GCACTAATAC ACCAGTATTT GTTGGATGCC TTCTTCACAC GGCCCTTTTA TAAAGCCCTT
    CTCAGAATTC TGTGTGACCT GAGTGATCTA GAATACCTTG ACGAAGAGTT CCACCAGAGC
    CTGCAGTGGA TGAAAGACAA TGACATCCAT GACATCTTGG ACCTTACATT CACTGTGAAT
    GAAGAAGTTT TTGGGCAGAT TACCGAACGA GAATTAAAAC CAGGGGGTGC CAATATCCCA
    GTCACAGAGA AGAACAAGAA GGAGTACATC GAAAGGATGG TGAAATGGAG GATTGAGAGG
    GGCGTCGTAC AGCAAACGGA GAGCTTAGTG CGTGGCTTCT ATGAGGTGGT GGATGCCAGG
    CTGGTGTCTG TTTTTGATGC AAGAGAACTG GAACTGGTCA TCGCAGGCAC AGCTGAAATC
    GACCTGAGTG ACTGGAGAAA CAACACAGAA TATAGAGGAG GATATCATGA CAATCATATT
    GTAATTCGGT GGTTCTGGGC TGCAGTGGAA AGATTCAACA ATGAACAACG ACTAAGGTTG
    TTACAGTTTG TCACGGGCAC ATCCAGCATT CCCTATGAAG GATTTGCTTC TCTCCGAGGG
    AGTAATGGCC CAAGAAGATT CTGCGTGGAG AAATGGGGGA AAATCACTGC TCTTCCCAGA
    GCTCACACTT GTTTTAACCG TCTGGATCTC CCCCCATACC CATCCTTCTC CATGCTTTAT
    GAAAAACTCT TGACAGCAGT TGAAGAGACC AGCACCTTTG GACTTGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.