Hecw2 cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Hecw2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hecw2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa06919 NM_001108218.1
Latest version!
Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
ORa06919 XM_017596447.1
Latest version!
Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
ORa32194 XM_006244912.3
Latest version!
Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa06919
    Clone ID Related Accession (Same CDS sequence) NM_001108218.1 , XM_017596447.1
    Accession Version NM_001108218.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-27
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase HECW2
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473965.2. On or before Oct 4, 2007 this sequence version replaced XM_237139.4, XM_001062004.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCAAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
    TACACACTGA GCCCAGAGAA CCTTCAGAGC CTCGCAACCC AGAACTCAAT GCCCGAGAAC
    ATGGCCCTGC AGCGAGCCAA CAGTGACACC GACCTCGTGA CCTCCGAGAG CCGCTCCAGC
    CTGACTGCCA GCATGTATGA GTACACACTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATCGGAC TCTATCACAT AGACGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGCGTGA CTGGAACCCA AAAAGGACAA
    ATTGTATGGC GGATTGAGCC CGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC
    AAATACTACC ATGGCATTAG CGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACTGTGAAG
    AACCCGGCTG TGATGATGGG TGCAGAAGGC ATGGAAGGAT GTGCTTCAGG AAGCCTGCAC
    TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCTACCT GTGCCCACCA CGGGCAGGAG AGAAGATCCA CTATCATCAG TAACACCACC
    AATCCAATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACCGA TGTCTTAGAA
    ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGAAAA
    TTAACCATTC CAGTGCAGAG GCTGTTGGAG AGGCAGGCTG TCGGTGACCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCCGCTGAT CACGTGAGTG GCTACCTCCA GTTTAGAGTG
    GAAGTCCCAT CCTCTGCCCA GGAAGATGCA TCTCCAGAGG CCGTTGGCAC CATCCTCGGT
    GTCCACACTG TGAATGGAGA CCTGGGCAGC CCTTCTGATG ATGAGGACAT GCCAGGGAGC
    CATCATGACA GCACCATTTG TGCTAACGGG CCAGTGTCTG AGGACAGTGT TGCTGACGGA
    ACCCCCAAGC ATTCTTTCAG GACTAGCTCT ACCCTGGAGA TTGACACAGA GGACTTGATC
    TCTACTTCTT CAAGAAATTC ACCCCCCAGG GGCCGCCAGG ATTCACTCAA TGATTACTTA
    GATGCTATTG AACACAATGG CCCCTCTAGG CCAGGGACAG CCTCCTCCTC TGAGCGGCCC
    ATGGGAGCCT CTCCCAAACT GAGGAGTAGT TTTCCCACTG ACACCAGACT CAATGCGATG
    CTTCATATAG ACTCAGATGA AGAAGACCAT GATTTTCAGC AAGACCTAGG CTACCCATCC
    TCCTTGGAGG AGGAAGGAGG CCTGATCATG TGCAGCAGGA CATCGAGGAT CGATGATGGG
    AGCCTGACGT CTCAGACCAA ACCAGAGGAT GACAACCCTG TTGAGAACGA GGATGCCTCC
    GTACATGAAA CTGCTTCCTT AGAGGAGAGG CCAGAGAATC TTCCCGAGGT TGAAGATGGC
    TCCTTGCCGT CAGGTACAGC CCCTGATGAA AATGAAGCCA GCGTAGAGCC TCAGCCCAGT
    GCTGACCAGG GCAGCACTGA ATTGTGTAGC TCTCAAGAAG TCGATCAGCC CACAAGTGGC
    ACGGACGCAG GAGCTTCTGA CACATCTGTA GGAAGCCGCA GAGCTGCCAG TGAGACTGAG
    TCCCTGGACC AGGGATCCGA ACCTTCCCAG GTGTCCTCTG AGACAGAACC CAGTGACCCT
    GCAAGGACAG AGAGTGTGAG TGAGGCCAGT ACCAGACCCG AGGGAGAGAG TGACCTGGAA
    GGTGCAGACA GCTCATGCAA TGAGAGCGTG ACCACACAGC TGTCCTCCGT GGAGACACGG
    TGCTCATCTC TAGAGAGTGC ACGATTTCCA GAAACCCCGG CCTTTTCTTC TCAGGAAGAG
    GAAGATGGGG CCTGCGCAGC TGAGCCGACC AGCAGCGGCC CCACAGAGGG GTCGCAGGAG
    TCTGTCTGCA CTCCCGGTTC TTTACCTGCT GTGCAGGTGC CCAGCAGGGA AGAGGAAGGG
    TCGGGGGCTG AAGCTACTGC TTTATCTGAG CAGGGGGAGC TGGGGGAGGT CTGGCAGAGG
    AGGGGCAGTC TTGAGGGAGC AGCTGCTGCT GCCACTGCTG CTGCCACCGA TAGCCAGCCC
    CAGGAAGACG GGGATCCTGG TGATACACAA GGTGCCTGCG AAGGGGCCAC CGCTCAGGAG
    GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCATCAGC CCTTGAGGTC ACTACCTTCA
    GTGCGCCAGG ACGTCAGCCG ATACCAGAGG GTGGACGAGG CTCTCCCACC AAACTGGGAA
    GCACGTATTG ACAGCCACGG CAGAATCTTC TACGTGGACC ATGTAAACAG AACCACGACG
    TGGCAGCGGC CTACAGCTCC CCCTGCCCCA CAGGTCCTGC AGAGATCTAA CTCCATACAG
    CAAATGGAGC AGCTAAACCG GCGTTATCAG AGCATCCGCA GAACCATGAC GAATGAGAGG
    CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAAG AAGCCGACTT TCACCAAGCC
    AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCCCACTCTA CCTCCAGATC CAGGCTCACG
    CTGCTGTTAC AGTCGCCCCC TGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG
    CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC
    ACCAAAGTCC GGCGGGACAC CCACCACTTT GAACGCTATC AGCATAACAG GGACCTCGTG
    GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA
    CACGATCATC AGGGCAAGGC ATTTTTTGTC GACCACAACT CCCGAACCAC CACGTTCATT
    GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACGAGTG CGCTGGTTCA TCGACAACAC
    CTGACGAGGC AACGCAGTCA CAGCGCGGGT GAGGTAGGAG AAGACTCTCG GCATGCAGGA
    CCACCCGTTC TTCCCAGGCC AGCCAGTACA TTCAATACGG TCAGTAGGCC ACAGTACCAG
    GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCTAACATC
    CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG
    ATCCAATTTA TCCGAACTGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG TGATGCAGAT
    CTTGTCATGT TACTGAGCTT ATTTGAGGAA GAAATAATGT CATATGTGCC TCCTCATGCC
    TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CTGTGTCATC TCCCCAGAAT
    TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCTGCAC CTTACAAGAG AGATTTTGAA
    GCCAAACTAA GGAACTTTTA TAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA
    AAATTAAAGT TAATTATCCG AAGAGACCAC TTGCTGGAAG ATGCTTTTAA TCAGATTATG
    GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA
    GGGTTGGATT ACAGTGGGCC TTCCAGAGAG TTTTTCTTCC TGGTATCCCG AGAACTCTTT
    AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATACACAGT ACAAATAAGT
    CCTATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTTC GATTCAGTGG GAGGATCCTC
    GGTCTTGCAC TAATACACCA GTACTTGTTG GACGCCTTCT TTACGCGACC CTTCTATAAG
    GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC
    CAGAGCCTTC AATGGATGAA AGACAATGAC ATCCATGACA TCCTAGACCT CACGTTCACT
    GTGAACGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT
    ATTCCAGTGA CGGAGAAGAA CAAGAAGGAG TACATTGAGA GGATGGTGAA GTGGAGGATT
    GAGAGGGGCG TGGTGCAGCA GACAGAGAGC TTAGTCCGCG GTTTCTATGA GGTAGTAGAT
    GCCAGGCTAG TGTCTGTCTT TGATGCAAGA GAACTGGAGC TGGTCATTGC TGGCACAGCT
    GAGATAGATC TCAATGACTG GAGGAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT
    CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTAGAACGAT TCAACAATGA ACAACGACTA
    AGGTTGTTAC AGTTTGTTAC AGGCACATCT AGCATTCCCT ATGAAGGATT TGCTTCTCTC
    CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT
    CCCAGAGCTC ACACGTGTTT TAACCGCCTG GATCTCCCTC CATACCCTTC CTTTTCCATG
    CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTCGGACT CGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001101688.1
    CDS40..4776
    Translation

    Target ORF information:

    RefSeq Version NM_001108218.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001108218.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCAAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
    TACACACTGA GCCCAGAGAA CCTTCAGAGC CTCGCAACCC AGAACTCAAT GCCCGAGAAC
    ATGGCCCTGC AGCGAGCCAA CAGTGACACC GACCTCGTGA CCTCCGAGAG CCGCTCCAGC
    CTGACTGCCA GCATGTATGA GTACACACTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATCGGAC TCTATCACAT AGACGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGCGTGA CTGGAACCCA AAAAGGACAA
    ATTGTATGGC GGATTGAGCC CGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC
    AAATACTACC ATGGCATTAG CGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACTGTGAAG
    AACCCGGCTG TGATGATGGG TGCAGAAGGC ATGGAAGGAT GTGCTTCAGG AAGCCTGCAC
    TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCTACCT GTGCCCACCA CGGGCAGGAG AGAAGATCCA CTATCATCAG TAACACCACC
    AATCCAATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACCGA TGTCTTAGAA
    ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGAAAA
    TTAACCATTC CAGTGCAGAG GCTGTTGGAG AGGCAGGCTG TCGGTGACCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCCGCTGAT CACGTGAGTG GCTACCTCCA GTTTAGAGTG
    GAAGTCCCAT CCTCTGCCCA GGAAGATGCA TCTCCAGAGG CCGTTGGCAC CATCCTCGGT
    GTCCACACTG TGAATGGAGA CCTGGGCAGC CCTTCTGATG ATGAGGACAT GCCAGGGAGC
    CATCATGACA GCACCATTTG TGCTAACGGG CCAGTGTCTG AGGACAGTGT TGCTGACGGA
    ACCCCCAAGC ATTCTTTCAG GACTAGCTCT ACCCTGGAGA TTGACACAGA GGACTTGATC
    TCTACTTCTT CAAGAAATTC ACCCCCCAGG GGCCGCCAGG ATTCACTCAA TGATTACTTA
    GATGCTATTG AACACAATGG CCCCTCTAGG CCAGGGACAG CCTCCTCCTC TGAGCGGCCC
    ATGGGAGCCT CTCCCAAACT GAGGAGTAGT TTTCCCACTG ACACCAGACT CAATGCGATG
    CTTCATATAG ACTCAGATGA AGAAGACCAT GATTTTCAGC AAGACCTAGG CTACCCATCC
    TCCTTGGAGG AGGAAGGAGG CCTGATCATG TGCAGCAGGA CATCGAGGAT CGATGATGGG
    AGCCTGACGT CTCAGACCAA ACCAGAGGAT GACAACCCTG TTGAGAACGA GGATGCCTCC
    GTACATGAAA CTGCTTCCTT AGAGGAGAGG CCAGAGAATC TTCCCGAGGT TGAAGATGGC
    TCCTTGCCGT CAGGTACAGC CCCTGATGAA AATGAAGCCA GCGTAGAGCC TCAGCCCAGT
    GCTGACCAGG GCAGCACTGA ATTGTGTAGC TCTCAAGAAG TCGATCAGCC CACAAGTGGC
    ACGGACGCAG GAGCTTCTGA CACATCTGTA GGAAGCCGCA GAGCTGCCAG TGAGACTGAG
    TCCCTGGACC AGGGATCCGA ACCTTCCCAG GTGTCCTCTG AGACAGAACC CAGTGACCCT
    GCAAGGACAG AGAGTGTGAG TGAGGCCAGT ACCAGACCCG AGGGAGAGAG TGACCTGGAA
    GGTGCAGACA GCTCATGCAA TGAGAGCGTG ACCACACAGC TGTCCTCCGT GGAGACACGG
    TGCTCATCTC TAGAGAGTGC ACGATTTCCA GAAACCCCGG CCTTTTCTTC TCAGGAAGAG
    GAAGATGGGG CCTGCGCAGC TGAGCCGACC AGCAGCGGCC CCACAGAGGG GTCGCAGGAG
    TCTGTCTGCA CTCCCGGTTC TTTACCTGCT GTGCAGGTGC CCAGCAGGGA AGAGGAAGGG
    TCGGGGGCTG AAGCTACTGC TTTATCTGAG CAGGGGGAGC TGGGGGAGGT CTGGCAGAGG
    AGGGGCAGTC TTGAGGGAGC AGCTGCTGCT GCCACTGCTG CTGCCACCGA TAGCCAGCCC
    CAGGAAGACG GGGATCCTGG TGATACACAA GGTGCCTGCG AAGGGGCCAC CGCTCAGGAG
    GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCATCAGC CCTTGAGGTC ACTACCTTCA
    GTGCGCCAGG ACGTCAGCCG ATACCAGAGG GTGGACGAGG CTCTCCCACC AAACTGGGAA
    GCACGTATTG ACAGCCACGG CAGAATCTTC TACGTGGACC ATGTAAACAG AACCACGACG
    TGGCAGCGGC CTACAGCTCC CCCTGCCCCA CAGGTCCTGC AGAGATCTAA CTCCATACAG
    CAAATGGAGC AGCTAAACCG GCGTTATCAG AGCATCCGCA GAACCATGAC GAATGAGAGG
    CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAAG AAGCCGACTT TCACCAAGCC
    AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCCCACTCTA CCTCCAGATC CAGGCTCACG
    CTGCTGTTAC AGTCGCCCCC TGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG
    CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC
    ACCAAAGTCC GGCGGGACAC CCACCACTTT GAACGCTATC AGCATAACAG GGACCTCGTG
    GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA
    CACGATCATC AGGGCAAGGC ATTTTTTGTC GACCACAACT CCCGAACCAC CACGTTCATT
    GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACGAGTG CGCTGGTTCA TCGACAACAC
    CTGACGAGGC AACGCAGTCA CAGCGCGGGT GAGGTAGGAG AAGACTCTCG GCATGCAGGA
    CCACCCGTTC TTCCCAGGCC AGCCAGTACA TTCAATACGG TCAGTAGGCC ACAGTACCAG
    GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCTAACATC
    CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG
    ATCCAATTTA TCCGAACTGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG TGATGCAGAT
    CTTGTCATGT TACTGAGCTT ATTTGAGGAA GAAATAATGT CATATGTGCC TCCTCATGCC
    TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CTGTGTCATC TCCCCAGAAT
    TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCTGCAC CTTACAAGAG AGATTTTGAA
    GCCAAACTAA GGAACTTTTA TAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA
    AAATTAAAGT TAATTATCCG AAGAGACCAC TTGCTGGAAG ATGCTTTTAA TCAGATTATG
    GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA
    GGGTTGGATT ACAGTGGGCC TTCCAGAGAG TTTTTCTTCC TGGTATCCCG AGAACTCTTT
    AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATACACAGT ACAAATAAGT
    CCTATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTTC GATTCAGTGG GAGGATCCTC
    GGTCTTGCAC TAATACACCA GTACTTGTTG GACGCCTTCT TTACGCGACC CTTCTATAAG
    GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC
    CAGAGCCTTC AATGGATGAA AGACAATGAC ATCCATGACA TCCTAGACCT CACGTTCACT
    GTGAACGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT
    ATTCCAGTGA CGGAGAAGAA CAAGAAGGAG TACATTGAGA GGATGGTGAA GTGGAGGATT
    GAGAGGGGCG TGGTGCAGCA GACAGAGAGC TTAGTCCGCG GTTTCTATGA GGTAGTAGAT
    GCCAGGCTAG TGTCTGTCTT TGATGCAAGA GAACTGGAGC TGGTCATTGC TGGCACAGCT
    GAGATAGATC TCAATGACTG GAGGAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT
    CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTAGAACGAT TCAACAATGA ACAACGACTA
    AGGTTGTTAC AGTTTGTTAC AGGCACATCT AGCATTCCCT ATGAAGGATT TGCTTCTCTC
    CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT
    CCCAGAGCTC ACACGTGTTT TAACCGCCTG GATCTCCCTC CATACCCTTC CTTTTCCATG
    CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTCGGACT CGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa06919
    Clone ID Related Accession (Same CDS sequence) NM_001108218.1 , XM_017596447.1
    Accession Version XM_017596447.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4737bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase HECW2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCAAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
    TACACACTGA GCCCAGAGAA CCTTCAGAGC CTCGCAACCC AGAACTCAAT GCCCGAGAAC
    ATGGCCCTGC AGCGAGCCAA CAGTGACACC GACCTCGTGA CCTCCGAGAG CCGCTCCAGC
    CTGACTGCCA GCATGTATGA GTACACACTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATCGGAC TCTATCACAT AGACGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGCGTGA CTGGAACCCA AAAAGGACAA
    ATTGTATGGC GGATTGAGCC CGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC
    AAATACTACC ATGGCATTAG CGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACTGTGAAG
    AACCCGGCTG TGATGATGGG TGCAGAAGGC ATGGAAGGAT GTGCTTCAGG AAGCCTGCAC
    TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCTACCT GTGCCCACCA CGGGCAGGAG AGAAGATCCA CTATCATCAG TAACACCACC
    AATCCAATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACCGA TGTCTTAGAA
    ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGAAAA
    TTAACCATTC CAGTGCAGAG GCTGTTGGAG AGGCAGGCTG TCGGTGACCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCCGCTGAT CACGTGAGTG GCTACCTCCA GTTTAGAGTG
    GAAGTCCCAT CCTCTGCCCA GGAAGATGCA TCTCCAGAGG CCGTTGGCAC CATCCTCGGT
    GTCCACACTG TGAATGGAGA CCTGGGCAGC CCTTCTGATG ATGAGGACAT GCCAGGGAGC
    CATCATGACA GCACCATTTG TGCTAACGGG CCAGTGTCTG AGGACAGTGT TGCTGACGGA
    ACCCCCAAGC ATTCTTTCAG GACTAGCTCT ACCCTGGAGA TTGACACAGA GGACTTGATC
    TCTACTTCTT CAAGAAATTC ACCCCCCAGG GGCCGCCAGG ATTCACTCAA TGATTACTTA
    GATGCTATTG AACACAATGG CCCCTCTAGG CCAGGGACAG CCTCCTCCTC TGAGCGGCCC
    ATGGGAGCCT CTCCCAAACT GAGGAGTAGT TTTCCCACTG ACACCAGACT CAATGCGATG
    CTTCATATAG ACTCAGATGA AGAAGACCAT GATTTTCAGC AAGACCTAGG CTACCCATCC
    TCCTTGGAGG AGGAAGGAGG CCTGATCATG TGCAGCAGGA CATCGAGGAT CGATGATGGG
    AGCCTGACGT CTCAGACCAA ACCAGAGGAT GACAACCCTG TTGAGAACGA GGATGCCTCC
    GTACATGAAA CTGCTTCCTT AGAGGAGAGG CCAGAGAATC TTCCCGAGGT TGAAGATGGC
    TCCTTGCCGT CAGGTACAGC CCCTGATGAA AATGAAGCCA GCGTAGAGCC TCAGCCCAGT
    GCTGACCAGG GCAGCACTGA ATTGTGTAGC TCTCAAGAAG TCGATCAGCC CACAAGTGGC
    ACGGACGCAG GAGCTTCTGA CACATCTGTA GGAAGCCGCA GAGCTGCCAG TGAGACTGAG
    TCCCTGGACC AGGGATCCGA ACCTTCCCAG GTGTCCTCTG AGACAGAACC CAGTGACCCT
    GCAAGGACAG AGAGTGTGAG TGAGGCCAGT ACCAGACCCG AGGGAGAGAG TGACCTGGAA
    GGTGCAGACA GCTCATGCAA TGAGAGCGTG ACCACACAGC TGTCCTCCGT GGAGACACGG
    TGCTCATCTC TAGAGAGTGC ACGATTTCCA GAAACCCCGG CCTTTTCTTC TCAGGAAGAG
    GAAGATGGGG CCTGCGCAGC TGAGCCGACC AGCAGCGGCC CCACAGAGGG GTCGCAGGAG
    TCTGTCTGCA CTCCCGGTTC TTTACCTGCT GTGCAGGTGC CCAGCAGGGA AGAGGAAGGG
    TCGGGGGCTG AAGCTACTGC TTTATCTGAG CAGGGGGAGC TGGGGGAGGT CTGGCAGAGG
    AGGGGCAGTC TTGAGGGAGC AGCTGCTGCT GCCACTGCTG CTGCCACCGA TAGCCAGCCC
    CAGGAAGACG GGGATCCTGG TGATACACAA GGTGCCTGCG AAGGGGCCAC CGCTCAGGAG
    GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCATCAGC CCTTGAGGTC ACTACCTTCA
    GTGCGCCAGG ACGTCAGCCG ATACCAGAGG GTGGACGAGG CTCTCCCACC AAACTGGGAA
    GCACGTATTG ACAGCCACGG CAGAATCTTC TACGTGGACC ATGTAAACAG AACCACGACG
    TGGCAGCGGC CTACAGCTCC CCCTGCCCCA CAGGTCCTGC AGAGATCTAA CTCCATACAG
    CAAATGGAGC AGCTAAACCG GCGTTATCAG AGCATCCGCA GAACCATGAC GAATGAGAGG
    CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAAG AAGCCGACTT TCACCAAGCC
    AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCCCACTCTA CCTCCAGATC CAGGCTCACG
    CTGCTGTTAC AGTCGCCCCC TGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG
    CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC
    ACCAAAGTCC GGCGGGACAC CCACCACTTT GAACGCTATC AGCATAACAG GGACCTCGTG
    GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA
    CACGATCATC AGGGCAAGGC ATTTTTTGTC GACCACAACT CCCGAACCAC CACGTTCATT
    GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACGAGTG CGCTGGTTCA TCGACAACAC
    CTGACGAGGC AACGCAGTCA CAGCGCGGGT GAGGTAGGAG AAGACTCTCG GCATGCAGGA
    CCACCCGTTC TTCCCAGGCC AGCCAGTACA TTCAATACGG TCAGTAGGCC ACAGTACCAG
    GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCTAACATC
    CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG
    ATCCAATTTA TCCGAACTGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG TGATGCAGAT
    CTTGTCATGT TACTGAGCTT ATTTGAGGAA GAAATAATGT CATATGTGCC TCCTCATGCC
    TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CTGTGTCATC TCCCCAGAAT
    TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCTGCAC CTTACAAGAG AGATTTTGAA
    GCCAAACTAA GGAACTTTTA TAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA
    AAATTAAAGT TAATTATCCG AAGAGACCAC TTGCTGGAAG ATGCTTTTAA TCAGATTATG
    GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA
    GGGTTGGATT ACAGTGGGCC TTCCAGAGAG TTTTTCTTCC TGGTATCCCG AGAACTCTTT
    AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATACACAGT ACAAATAAGT
    CCTATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTTC GATTCAGTGG GAGGATCCTC
    GGTCTTGCAC TAATACACCA GTACTTGTTG GACGCCTTCT TTACGCGACC CTTCTATAAG
    GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC
    CAGAGCCTTC AATGGATGAA AGACAATGAC ATCCATGACA TCCTAGACCT CACGTTCACT
    GTGAACGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT
    ATTCCAGTGA CGGAGAAGAA CAAGAAGGAG TACATTGAGA GGATGGTGAA GTGGAGGATT
    GAGAGGGGCG TGGTGCAGCA GACAGAGAGC TTAGTCCGCG GTTTCTATGA GGTAGTAGAT
    GCCAGGCTAG TGTCTGTCTT TGATGCAAGA GAACTGGAGC TGGTCATTGC TGGCACAGCT
    GAGATAGATC TCAATGACTG GAGGAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT
    CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTAGAACGAT TCAACAATGA ACAACGACTA
    AGGTTGTTAC AGTTTGTTAC AGGCACATCT AGCATTCCCT ATGAAGGATT TGCTTCTCTC
    CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT
    CCCAGAGCTC ACACGTGTTT TAACCGCCTG GATCTCCCTC CATACCCTTC CTTTTCCATG
    CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTCGGACT CGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017451936.1
    CDS92..4828
    Translation

    Target ORF information:

    RefSeq Version XM_017596447.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017596447.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCAAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG 
    TACACACTGA GCCCAGAGAA CCTTCAGAGC CTCGCAACCC AGAACTCAAT GCCCGAGAAC
    ATGGCCCTGC AGCGAGCCAA CAGTGACACC GACCTCGTGA CCTCCGAGAG CCGCTCCAGC
    CTGACTGCCA GCATGTATGA GTACACACTG GGGCAAGCCC AGAACCTCAT TATCTTCTGG
    GACATTAAGG AAGAGGTTGA CCCCAGCGAT TGGATCGGAC TCTATCACAT AGACGAGAAT
    TCTCCAGCCA ACTTCTGGGA TTCTAAAAAC AGGGGCGTGA CTGGAACCCA AAAAGGACAA
    ATTGTATGGC GGATTGAGCC CGGGCCCTAC TTCATGGAAC CGGAGATCAA AATCTGTTTC
    AAATACTACC ATGGCATTAG CGGAGCCCTG CGAGCCACAA CCCCCTGTAT TACTGTGAAG
    AACCCGGCTG TGATGATGGG TGCAGAAGGC ATGGAAGGAT GTGCTTCAGG AAGCCTGCAC
    TCTAGGAAAC TCGTCAGCTT CACATTGTCA GATCTCAGGG CAGTTGGGCT CAAGAAAGGG
    ATGTTCTTCA ATCCTGACCC TTATCTTAAG ATGTCCATTC AGCCAGGGAA GAAGAGCAGT
    TTCCCTACCT GTGCCCACCA CGGGCAGGAG AGAAGATCCA CTATCATCAG TAACACCACC
    AATCCAATCT GGCACAGAGA GAAATACTCC TTTTTTGCAC TTTTGACCGA TGTCTTAGAA
    ATTGAAATCA AAGACAAATT TGCCAAGAGC CGGCCCATCA TCAAACGCTT CCTGGGAAAA
    TTAACCATTC CAGTGCAGAG GCTGTTGGAG AGGCAGGCTG TCGGTGACCA GATGCTCAGC
    TACAACCTTG GCAGAAGGCT CCCCGCTGAT CACGTGAGTG GCTACCTCCA GTTTAGAGTG
    GAAGTCCCAT CCTCTGCCCA GGAAGATGCA TCTCCAGAGG CCGTTGGCAC CATCCTCGGT
    GTCCACACTG TGAATGGAGA CCTGGGCAGC CCTTCTGATG ATGAGGACAT GCCAGGGAGC
    CATCATGACA GCACCATTTG TGCTAACGGG CCAGTGTCTG AGGACAGTGT TGCTGACGGA
    ACCCCCAAGC ATTCTTTCAG GACTAGCTCT ACCCTGGAGA TTGACACAGA GGACTTGATC
    TCTACTTCTT CAAGAAATTC ACCCCCCAGG GGCCGCCAGG ATTCACTCAA TGATTACTTA
    GATGCTATTG AACACAATGG CCCCTCTAGG CCAGGGACAG CCTCCTCCTC TGAGCGGCCC
    ATGGGAGCCT CTCCCAAACT GAGGAGTAGT TTTCCCACTG ACACCAGACT CAATGCGATG
    CTTCATATAG ACTCAGATGA AGAAGACCAT GATTTTCAGC AAGACCTAGG CTACCCATCC
    TCCTTGGAGG AGGAAGGAGG CCTGATCATG TGCAGCAGGA CATCGAGGAT CGATGATGGG
    AGCCTGACGT CTCAGACCAA ACCAGAGGAT GACAACCCTG TTGAGAACGA GGATGCCTCC
    GTACATGAAA CTGCTTCCTT AGAGGAGAGG CCAGAGAATC TTCCCGAGGT TGAAGATGGC
    TCCTTGCCGT CAGGTACAGC CCCTGATGAA AATGAAGCCA GCGTAGAGCC TCAGCCCAGT
    GCTGACCAGG GCAGCACTGA ATTGTGTAGC TCTCAAGAAG TCGATCAGCC CACAAGTGGC
    ACGGACGCAG GAGCTTCTGA CACATCTGTA GGAAGCCGCA GAGCTGCCAG TGAGACTGAG
    TCCCTGGACC AGGGATCCGA ACCTTCCCAG GTGTCCTCTG AGACAGAACC CAGTGACCCT
    GCAAGGACAG AGAGTGTGAG TGAGGCCAGT ACCAGACCCG AGGGAGAGAG TGACCTGGAA
    GGTGCAGACA GCTCATGCAA TGAGAGCGTG ACCACACAGC TGTCCTCCGT GGAGACACGG
    TGCTCATCTC TAGAGAGTGC ACGATTTCCA GAAACCCCGG CCTTTTCTTC TCAGGAAGAG
    GAAGATGGGG CCTGCGCAGC TGAGCCGACC AGCAGCGGCC CCACAGAGGG GTCGCAGGAG
    TCTGTCTGCA CTCCCGGTTC TTTACCTGCT GTGCAGGTGC CCAGCAGGGA AGAGGAAGGG
    TCGGGGGCTG AAGCTACTGC TTTATCTGAG CAGGGGGAGC TGGGGGAGGT CTGGCAGAGG
    AGGGGCAGTC TTGAGGGAGC AGCTGCTGCT GCCACTGCTG CTGCCACCGA TAGCCAGCCC
    CAGGAAGACG GGGATCCTGG TGATACACAA GGTGCCTGCG AAGGGGCCAC CGCTCAGGAG
    GAGGGCGCCA CTGGAGGTTC TCAAACCAAT GGCCATCAGC CCTTGAGGTC ACTACCTTCA
    GTGCGCCAGG ACGTCAGCCG ATACCAGAGG GTGGACGAGG CTCTCCCACC AAACTGGGAA
    GCACGTATTG ACAGCCACGG CAGAATCTTC TACGTGGACC ATGTAAACAG AACCACGACG
    TGGCAGCGGC CTACAGCTCC CCCTGCCCCA CAGGTCCTGC AGAGATCTAA CTCCATACAG
    CAAATGGAGC AGCTAAACCG GCGTTATCAG AGCATCCGCA GAACCATGAC GAATGAGAGG
    CCTGAGGAAA ATACCAGTGC TATCGATGGG GCAGGGGAAG AAGCCGACTT TCACCAAGCC
    AGCGCAGACT TCCGTCGGGA GAACGTCCTG CCCCACTCTA CCTCCAGATC CAGGCTCACG
    CTGCTGTTAC AGTCGCCCCC TGTGAAGTTC CTCATCAGCC CAGAGTTCTT CACCGTGCTG
    CATTCTAACC CTAGTGCCTA CCGCATGTTT ACAAACAACA CGTGTTTGAA GCACATGATC
    ACCAAAGTCC GGCGGGACAC CCACCACTTT GAACGCTATC AGCATAACAG GGACCTCGTG
    GGATTCCTCA ACATGTTTGC TAACAAACAG CTGGAACTAC CAAGGGGCTG GGAGATGAAA
    CACGATCATC AGGGCAAGGC ATTTTTTGTC GACCACAACT CCCGAACCAC CACGTTCATT
    GATCCTCGGC TCCCACTTCA GAGCAGTAGG CCCACGAGTG CGCTGGTTCA TCGACAACAC
    CTGACGAGGC AACGCAGTCA CAGCGCGGGT GAGGTAGGAG AAGACTCTCG GCATGCAGGA
    CCACCCGTTC TTCCCAGGCC AGCCAGTACA TTCAATACGG TCAGTAGGCC ACAGTACCAG
    GACATGGTTC CAGTGGCCTA CAATGACAAA ATTGTTGCGT TTTTGCGTCA ACCTAACATC
    CTTGAAATTC TGCAAGAGCG TCAACCTGAT CTTGCCAGAA ACCACTCACT CAGGGAGAAG
    ATCCAATTTA TCCGAACTGA AGGGACCCCT GGATTGGTGC GGCTCTCAAG TGATGCAGAT
    CTTGTCATGT TACTGAGCTT ATTTGAGGAA GAAATAATGT CATATGTGCC TCCTCATGCC
    TTACTCCACC CCAGCTACTG TCAGTCCCCG CGTGGCTCCC CTGTGTCATC TCCCCAGAAT
    TCACCAGGTA CTCAGCGTGC CAATGCCCGG GCTCCTGCAC CTTACAAGAG AGATTTTGAA
    GCCAAACTAA GGAACTTTTA TAGGAAGTTA GAAACTAAAG GATACGGACA AGGCCCAGGA
    AAATTAAAGT TAATTATCCG AAGAGACCAC TTGCTGGAAG ATGCTTTTAA TCAGATTATG
    GGCTACTCCA GAAAGGACCT GCAGAGGAAT AAACTGTACG TCACCTTTGT CGGGGAGGAA
    GGGTTGGATT ACAGTGGGCC TTCCAGAGAG TTTTTCTTCC TGGTATCCCG AGAACTCTTT
    AACCCCTATT ACGGCTTATT TGAATATTCA GCCAATGACA CATACACAGT ACAAATAAGT
    CCTATGTCTG CCTTTGTAGA CAATCATCAT GAATGGTTTC GATTCAGTGG GAGGATCCTC
    GGTCTTGCAC TAATACACCA GTACTTGTTG GACGCCTTCT TTACGCGACC CTTCTATAAG
    GCTCTTCTCA GAATCCTGTG TGACCTGAGT GATCTGGAAT ACCTTGATGA GGAGTTCCAC
    CAGAGCCTTC AATGGATGAA AGACAATGAC ATCCATGACA TCCTAGACCT CACGTTCACT
    GTGAACGAAG AAGTTTTTGG GCAGATTACT GAACGAGAGT TAAAACCAGG GGGAGCCAAT
    ATTCCAGTGA CGGAGAAGAA CAAGAAGGAG TACATTGAGA GGATGGTGAA GTGGAGGATT
    GAGAGGGGCG TGGTGCAGCA GACAGAGAGC TTAGTCCGCG GTTTCTATGA GGTAGTAGAT
    GCCAGGCTAG TGTCTGTCTT TGATGCAAGA GAACTGGAGC TGGTCATTGC TGGCACAGCT
    GAGATAGATC TCAATGACTG GAGGAACAAC ACTGAATACA GAGGCGGGTA CCATGACAAT
    CACATTGTAA TTCGGTGGTT CTGGGCTGCA GTAGAACGAT TCAACAATGA ACAACGACTA
    AGGTTGTTAC AGTTTGTTAC AGGCACATCT AGCATTCCCT ATGAAGGATT TGCTTCTCTC
    CGAGGGAGTA ATGGTCCAAG AAGATTCTGT GTGGAAAAAT GGGGAAAAAT CACCGCTCTT
    CCCAGAGCTC ACACGTGTTT TAACCGCCTG GATCTCCCTC CATACCCTTC CTTTTCCATG
    CTTTATGAAA AACTCTTGAC AGCAGTTGAA GAGACCAGTA CCTTCGGACT CGAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa32194
    Clone ID Related Accession (Same CDS sequence) XM_006244912.3
    Accession Version XM_006244912.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4344bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product E3 ubiquitin-protein ligase HECW2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244912.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGAACCGG AGATCAAAAT CTGTTTCAAA TACTACCATG GCATTAGCGG AGCCCTGCGA 
    GCCACAACCC CCTGTATTAC TGTGAAGAAC CCGGCTGTGA TGATGGGTGC AGAAGGCATG
    GAAGGATGTG CTTCAGGAAG CCTGCACTCT AGGAAACTCG TCAGCTTCAC ATTGTCAGAT
    CTCAGGGCAG TTGGGCTCAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG
    TCCATTCAGC CAGGGAAGAA GAGCAGTTTC CCTACCTGTG CCCACCACGG GCAGGAGAGA
    AGATCCACTA TCATCAGTAA CACCACCAAT CCAATCTGGC ACAGAGAGAA ATACTCCTTT
    TTTGCACTTT TGACCGATGT CTTAGAAATT GAAATCAAAG ACAAATTTGC CAAGAGCCGG
    CCCATCATCA AACGCTTCCT GGGAAAATTA ACCATTCCAG TGCAGAGGCT GTTGGAGAGG
    CAGGCTGTCG GTGACCAGAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC CGCTGATCAC
    GTGAGTGGCT ACCTCCAGTT TAGAGTGGAA GTCCCATCCT CTGCCCAGGA AGATGCATCT
    CCAGAGGCCG TTGGCACCAT CCTCGGTGTC CACACTGTGA ATGGAGACCT GGGCAGCCCT
    TCTGATGATG AGGACATGCC AGGGAGCCAT CATGACAGCA CCATTTGTGC TAACGGGCCA
    GTGTCTGAGG ACAGTGTTGC TGACGGAACC CCCAAGCATT CTTTCAGGAC TAGCTCTACC
    CTGGAGATTG ACACAGAGGA CTTGATCTCT ACTTCTTCAA GAAATTCACC CCCCAGGGGC
    CGCCAGGATT CACTCAATGA TTACTTAGAT GCTATTGAAC ACAATGGCCC CTCTAGGCCA
    GGGACAGCCT CCTCCTCTGA GCGGCCCATG GGAGCCTCTC CCAAACTGAG GAGTAGTTTT
    CCCACTGACA CCAGACTCAA TGCGATGCTT CATATAGACT CAGATGAAGA AGACCATGAT
    TTTCAGCAAG ACCTAGGCTA CCCATCCTCC TTGGAGGAGG AAGGAGGCCT GATCATGTGC
    AGCAGGACAT CGAGGATCGA TGATGGGAGC CTGACGTCTC AGACCAAACC AGAGGATGAC
    AACCCTGTTG AGAACGAGGA TGCCTCCGTA CATGAAACTG CTTCCTTAGA GGAGAGGCCA
    GAGAATCTTC CCGAGGTTGA AGATGGCTCC TTGCCGTCAG GTACAGCCCC TGATGAAAAT
    GAAGCCAGCG TAGAGCCTCA GCCCAGTGCT GACCAGGGCA GCACTGAATT GTGTAGCTCT
    CAAGAAGTCG ATCAGCCCAC AAGTGGCACG GACGCAGGAG CTTCTGACAC ATCTGTAGGA
    AGCCGCAGAG CTGCCAGTGA GACTGAGTCC CTGGACCAGG GATCCGAACC TTCCCAGGTG
    TCCTCTGAGA CAGAACCCAG TGACCCTGCA AGGACAGAGA GTGTGAGTGA GGCCAGTACC
    AGACCCGAGG GAGAGAGTGA CCTGGAAGGT GCAGACAGCT CATGCAATGA GAGCGTGACC
    ACACAGCTGT CCTCCGTGGA GACACGGTGC TCATCTCTAG AGAGTGCACG ATTTCCAGAA
    ACCCCGGCCT TTTCTTCTCA GGAAGAGGAA GATGGGGCCT GCGCAGCTGA GCCGACCAGC
    AGCGGCCCCA CAGAGGGGTC GCAGGAGTCT GTCTGCACTC CCGGTTCTTT ACCTGCTGTG
    CAGGTGCCCA GCAGGGAAGA GGAAGGGTCG GGGGCTGAAG CTACTGCTTT ATCTGAGCAG
    GGGGAGCTGG GGGAGGTCTG GCAGAGGAGG GGCAGTCTTG AGGGAGCAGC TGCTGCTGCC
    ACTGCTGCTG CCACCGATAG CCAGCCCCAG GAAGACGGGG ATCCTGGTGA TACACAAGGT
    GCCTGCGAAG GGGCCACCGC TCAGGAGGAG GGCGCCACTG GAGGTTCTCA AACCAATGGC
    CATCAGCCCT TGAGGTCACT ACCTTCAGTG CGCCAGGACG TCAGCCGATA CCAGAGGGTG
    GACGAGGCTC TCCCACCAAA CTGGGAAGCA CGTATTGACA GCCACGGCAG AATCTTCTAC
    GTGGACCATG TAAACAGAAC CACGACGTGG CAGCGGCCTA CAGCTCCCCC TGCCCCACAG
    GTCCTGCAGA GATCTAACTC CATACAGCAA ATGGAGCAGC TAAACCGGCG TTATCAGAGC
    ATCCGCAGAA CCATGACGAA TGAGAGGCCT GAGGAAAATA CCAGTGCTAT CGATGGGGCA
    GGGGAAGAAG CCGACTTTCA CCAAGCCAGC GCAGACTTCC GTCGGGAGAA CGTCCTGCCC
    CACTCTACCT CCAGATCCAG GCTCACGCTG CTGTTACAGT CGCCCCCTGT GAAGTTCCTC
    ATCAGCCCAG AGTTCTTCAC CGTGCTGCAT TCTAACCCTA GTGCCTACCG CATGTTTACA
    AACAACACGT GTTTGAAGCA CATGATCACC AAAGTCCGGC GGGACACCCA CCACTTTGAA
    CGCTATCAGC ATAACAGGGA CCTCGTGGGA TTCCTCAACA TGTTTGCTAA CAAACAGCTG
    GAACTACCAA GGGGCTGGGA GATGAAACAC GATCATCAGG GCAAGGCATT TTTTGTCGAC
    CACAACTCCC GAACCACCAC GTTCATTGAT CCTCGGCTCC CACTTCAGAG CAGTAGGCCC
    ACGAGTGCGC TGGTTCATCG ACAACACCTG ACGAGGCAAC GCAGTCACAG CGCGGGTGAG
    GTAGGAGAAG ACTCTCGGCA TGCAGGACCA CCCGTTCTTC CCAGGCCAGC CAGTACATTC
    AATACGGTCA GTAGGCCACA GTACCAGGAC ATGGTTCCAG TGGCCTACAA TGACAAAATT
    GTTGCGTTTT TGCGTCAACC TAACATCCTT GAAATTCTGC AAGAGCGTCA ACCTGATCTT
    GCCAGAAACC ACTCACTCAG GGAGAAGATC CAATTTATCC GAACTGAAGG GACCCCTGGA
    TTGGTGCGGC TCTCAAGTGA TGCAGATCTT GTCATGTTAC TGAGCTTATT TGAGGAAGAA
    ATAATGTCAT ATGTGCCTCC TCATGCCTTA CTCCACCCCA GCTACTGTCA GTCCCCGCGT
    GGCTCCCCTG TGTCATCTCC CCAGAATTCA CCAGGTACTC AGCGTGCCAA TGCCCGGGCT
    CCTGCACCTT ACAAGAGAGA TTTTGAAGCC AAACTAAGGA ACTTTTATAG GAAGTTAGAA
    ACTAAAGGAT ACGGACAAGG CCCAGGAAAA TTAAAGTTAA TTATCCGAAG AGACCACTTG
    CTGGAAGATG CTTTTAATCA GATTATGGGC TACTCCAGAA AGGACCTGCA GAGGAATAAA
    CTGTACGTCA CCTTTGTCGG GGAGGAAGGG TTGGATTACA GTGGGCCTTC CAGAGAGTTT
    TTCTTCCTGG TATCCCGAGA ACTCTTTAAC CCCTATTACG GCTTATTTGA ATATTCAGCC
    AATGACACAT ACACAGTACA AATAAGTCCT ATGTCTGCCT TTGTAGACAA TCATCATGAA
    TGGTTTCGAT TCAGTGGGAG GATCCTCGGT CTTGCACTAA TACACCAGTA CTTGTTGGAC
    GCCTTCTTTA CGCGACCCTT CTATAAGGCT CTTCTCAGAA TCCTGTGTGA CCTGAGTGAT
    CTGGAATACC TTGATGAGGA GTTCCACCAG AGCCTTCAAT GGATGAAAGA CAATGACATC
    CATGACATCC TAGACCTCAC GTTCACTGTG AACGAAGAAG TTTTTGGGCA GATTACTGAA
    CGAGAGTTAA AACCAGGGGG AGCCAATATT CCAGTGACGG AGAAGAACAA GAAGGAGTAC
    ATTGAGAGGA TGGTGAAGTG GAGGATTGAG AGGGGCGTGG TGCAGCAGAC AGAGAGCTTA
    GTCCGCGGTT TCTATGAGGT AGTAGATGCC AGGCTAGTGT CTGTCTTTGA TGCAAGAGAA
    CTGGAGCTGG TCATTGCTGG CACAGCTGAG ATAGATCTCA ATGACTGGAG GAACAACACT
    GAATACAGAG GCGGGTACCA TGACAATCAC ATTGTAATTC GGTGGTTCTG GGCTGCAGTA
    GAACGATTCA ACAATGAACA ACGACTAAGG TTGTTACAGT TTGTTACAGG CACATCTAGC
    ATTCCCTATG AAGGATTTGC TTCTCTCCGA GGGAGTAATG GTCCAAGAAG ATTCTGTGTG
    GAAAAATGGG GAAAAATCAC CGCTCTTCCC AGAGCTCACA CGTGTTTTAA CCGCCTGGAT
    CTCCCTCCAT ACCCTTCCTT TTCCATGCTT TATGAAAAAC TCTTGACAGC AGTTGAAGAG
    ACCAGTACCT TCGGACTCGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006244974.1
    CDS614..4957
    Translation

    Target ORF information:

    RefSeq Version XM_006244912.3
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (Hecw2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006244912.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGAACCGG AGATCAAAAT CTGTTTCAAA TACTACCATG GCATTAGCGG AGCCCTGCGA 
    GCCACAACCC CCTGTATTAC TGTGAAGAAC CCGGCTGTGA TGATGGGTGC AGAAGGCATG
    GAAGGATGTG CTTCAGGAAG CCTGCACTCT AGGAAACTCG TCAGCTTCAC ATTGTCAGAT
    CTCAGGGCAG TTGGGCTCAA GAAAGGGATG TTCTTCAATC CTGACCCTTA TCTTAAGATG
    TCCATTCAGC CAGGGAAGAA GAGCAGTTTC CCTACCTGTG CCCACCACGG GCAGGAGAGA
    AGATCCACTA TCATCAGTAA CACCACCAAT CCAATCTGGC ACAGAGAGAA ATACTCCTTT
    TTTGCACTTT TGACCGATGT CTTAGAAATT GAAATCAAAG ACAAATTTGC CAAGAGCCGG
    CCCATCATCA AACGCTTCCT GGGAAAATTA ACCATTCCAG TGCAGAGGCT GTTGGAGAGG
    CAGGCTGTCG GTGACCAGAT GCTCAGCTAC AACCTTGGCA GAAGGCTCCC CGCTGATCAC
    GTGAGTGGCT ACCTCCAGTT TAGAGTGGAA GTCCCATCCT CTGCCCAGGA AGATGCATCT
    CCAGAGGCCG TTGGCACCAT CCTCGGTGTC CACACTGTGA ATGGAGACCT GGGCAGCCCT
    TCTGATGATG AGGACATGCC AGGGAGCCAT CATGACAGCA CCATTTGTGC TAACGGGCCA
    GTGTCTGAGG ACAGTGTTGC TGACGGAACC CCCAAGCATT CTTTCAGGAC TAGCTCTACC
    CTGGAGATTG ACACAGAGGA CTTGATCTCT ACTTCTTCAA GAAATTCACC CCCCAGGGGC
    CGCCAGGATT CACTCAATGA TTACTTAGAT GCTATTGAAC ACAATGGCCC CTCTAGGCCA
    GGGACAGCCT CCTCCTCTGA GCGGCCCATG GGAGCCTCTC CCAAACTGAG GAGTAGTTTT
    CCCACTGACA CCAGACTCAA TGCGATGCTT CATATAGACT CAGATGAAGA AGACCATGAT
    TTTCAGCAAG ACCTAGGCTA CCCATCCTCC TTGGAGGAGG AAGGAGGCCT GATCATGTGC
    AGCAGGACAT CGAGGATCGA TGATGGGAGC CTGACGTCTC AGACCAAACC AGAGGATGAC
    AACCCTGTTG AGAACGAGGA TGCCTCCGTA CATGAAACTG CTTCCTTAGA GGAGAGGCCA
    GAGAATCTTC CCGAGGTTGA AGATGGCTCC TTGCCGTCAG GTACAGCCCC TGATGAAAAT
    GAAGCCAGCG TAGAGCCTCA GCCCAGTGCT GACCAGGGCA GCACTGAATT GTGTAGCTCT
    CAAGAAGTCG ATCAGCCCAC AAGTGGCACG GACGCAGGAG CTTCTGACAC ATCTGTAGGA
    AGCCGCAGAG CTGCCAGTGA GACTGAGTCC CTGGACCAGG GATCCGAACC TTCCCAGGTG
    TCCTCTGAGA CAGAACCCAG TGACCCTGCA AGGACAGAGA GTGTGAGTGA GGCCAGTACC
    AGACCCGAGG GAGAGAGTGA CCTGGAAGGT GCAGACAGCT CATGCAATGA GAGCGTGACC
    ACACAGCTGT CCTCCGTGGA GACACGGTGC TCATCTCTAG AGAGTGCACG ATTTCCAGAA
    ACCCCGGCCT TTTCTTCTCA GGAAGAGGAA GATGGGGCCT GCGCAGCTGA GCCGACCAGC
    AGCGGCCCCA CAGAGGGGTC GCAGGAGTCT GTCTGCACTC CCGGTTCTTT ACCTGCTGTG
    CAGGTGCCCA GCAGGGAAGA GGAAGGGTCG GGGGCTGAAG CTACTGCTTT ATCTGAGCAG
    GGGGAGCTGG GGGAGGTCTG GCAGAGGAGG GGCAGTCTTG AGGGAGCAGC TGCTGCTGCC
    ACTGCTGCTG CCACCGATAG CCAGCCCCAG GAAGACGGGG ATCCTGGTGA TACACAAGGT
    GCCTGCGAAG GGGCCACCGC TCAGGAGGAG GGCGCCACTG GAGGTTCTCA AACCAATGGC
    CATCAGCCCT TGAGGTCACT ACCTTCAGTG CGCCAGGACG TCAGCCGATA CCAGAGGGTG
    GACGAGGCTC TCCCACCAAA CTGGGAAGCA CGTATTGACA GCCACGGCAG AATCTTCTAC
    GTGGACCATG TAAACAGAAC CACGACGTGG CAGCGGCCTA CAGCTCCCCC TGCCCCACAG
    GTCCTGCAGA GATCTAACTC CATACAGCAA ATGGAGCAGC TAAACCGGCG TTATCAGAGC
    ATCCGCAGAA CCATGACGAA TGAGAGGCCT GAGGAAAATA CCAGTGCTAT CGATGGGGCA
    GGGGAAGAAG CCGACTTTCA CCAAGCCAGC GCAGACTTCC GTCGGGAGAA CGTCCTGCCC
    CACTCTACCT CCAGATCCAG GCTCACGCTG CTGTTACAGT CGCCCCCTGT GAAGTTCCTC
    ATCAGCCCAG AGTTCTTCAC CGTGCTGCAT TCTAACCCTA GTGCCTACCG CATGTTTACA
    AACAACACGT GTTTGAAGCA CATGATCACC AAAGTCCGGC GGGACACCCA CCACTTTGAA
    CGCTATCAGC ATAACAGGGA CCTCGTGGGA TTCCTCAACA TGTTTGCTAA CAAACAGCTG
    GAACTACCAA GGGGCTGGGA GATGAAACAC GATCATCAGG GCAAGGCATT TTTTGTCGAC
    CACAACTCCC GAACCACCAC GTTCATTGAT CCTCGGCTCC CACTTCAGAG CAGTAGGCCC
    ACGAGTGCGC TGGTTCATCG ACAACACCTG ACGAGGCAAC GCAGTCACAG CGCGGGTGAG
    GTAGGAGAAG ACTCTCGGCA TGCAGGACCA CCCGTTCTTC CCAGGCCAGC CAGTACATTC
    AATACGGTCA GTAGGCCACA GTACCAGGAC ATGGTTCCAG TGGCCTACAA TGACAAAATT
    GTTGCGTTTT TGCGTCAACC TAACATCCTT GAAATTCTGC AAGAGCGTCA ACCTGATCTT
    GCCAGAAACC ACTCACTCAG GGAGAAGATC CAATTTATCC GAACTGAAGG GACCCCTGGA
    TTGGTGCGGC TCTCAAGTGA TGCAGATCTT GTCATGTTAC TGAGCTTATT TGAGGAAGAA
    ATAATGTCAT ATGTGCCTCC TCATGCCTTA CTCCACCCCA GCTACTGTCA GTCCCCGCGT
    GGCTCCCCTG TGTCATCTCC CCAGAATTCA CCAGGTACTC AGCGTGCCAA TGCCCGGGCT
    CCTGCACCTT ACAAGAGAGA TTTTGAAGCC AAACTAAGGA ACTTTTATAG GAAGTTAGAA
    ACTAAAGGAT ACGGACAAGG CCCAGGAAAA TTAAAGTTAA TTATCCGAAG AGACCACTTG
    CTGGAAGATG CTTTTAATCA GATTATGGGC TACTCCAGAA AGGACCTGCA GAGGAATAAA
    CTGTACGTCA CCTTTGTCGG GGAGGAAGGG TTGGATTACA GTGGGCCTTC CAGAGAGTTT
    TTCTTCCTGG TATCCCGAGA ACTCTTTAAC CCCTATTACG GCTTATTTGA ATATTCAGCC
    AATGACACAT ACACAGTACA AATAAGTCCT ATGTCTGCCT TTGTAGACAA TCATCATGAA
    TGGTTTCGAT TCAGTGGGAG GATCCTCGGT CTTGCACTAA TACACCAGTA CTTGTTGGAC
    GCCTTCTTTA CGCGACCCTT CTATAAGGCT CTTCTCAGAA TCCTGTGTGA CCTGAGTGAT
    CTGGAATACC TTGATGAGGA GTTCCACCAG AGCCTTCAAT GGATGAAAGA CAATGACATC
    CATGACATCC TAGACCTCAC GTTCACTGTG AACGAAGAAG TTTTTGGGCA GATTACTGAA
    CGAGAGTTAA AACCAGGGGG AGCCAATATT CCAGTGACGG AGAAGAACAA GAAGGAGTAC
    ATTGAGAGGA TGGTGAAGTG GAGGATTGAG AGGGGCGTGG TGCAGCAGAC AGAGAGCTTA
    GTCCGCGGTT TCTATGAGGT AGTAGATGCC AGGCTAGTGT CTGTCTTTGA TGCAAGAGAA
    CTGGAGCTGG TCATTGCTGG CACAGCTGAG ATAGATCTCA ATGACTGGAG GAACAACACT
    GAATACAGAG GCGGGTACCA TGACAATCAC ATTGTAATTC GGTGGTTCTG GGCTGCAGTA
    GAACGATTCA ACAATGAACA ACGACTAAGG TTGTTACAGT TTGTTACAGG CACATCTAGC
    ATTCCCTATG AAGGATTTGC TTCTCTCCGA GGGAGTAATG GTCCAAGAAG ATTCTGTGTG
    GAAAAATGGG GAAAAATCAC CGCTCTTCCC AGAGCTCACA CGTGTTTTAA CCGCCTGGAT
    CTCCCTCCAT ACCCTTCCTT TTCCATGCTT TATGAAAAAC TCTTGACAGC AGTTGAAGAG
    ACCAGTACCT TCGGACTCGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.