gRNAs
The following Hecw2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hecw2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa06919 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001108218.1 , XM_017596447.1 | |
| Accession Version | NM_001108218.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-05-27 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | E3 ubiquitin-protein ligase HECW2 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473965.2. On or before Oct 4, 2007 this sequence version replaced XM_237139.4, XM_001062004.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGGCAAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001101688.1 |
| CDS | 40..4776 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001108218.1 |
1 | ATGGCAAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa06919 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001108218.1 , XM_017596447.1 | |
| Accession Version | XM_017596447.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4737bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | E3 ubiquitin-protein ligase HECW2 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCAAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017451936.1 |
| CDS | 92..4828 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_017596447.1 |
1 | ATGGCAAGCT CAGCCCGGGA GCATTTGCTT TTTGTGCGGC GTCGAAATCC ACAGATGCGG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa32194 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006244912.3 | |
| Accession Version | XM_006244912.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | E3 ubiquitin-protein ligase HECW2 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006244912.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAACCGG AGATCAAAAT CTGTTTCAAA TACTACCATG GCATTAGCGG AGCCCTGCGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006244974.1 |
| CDS | 614..4957 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006244912.3 |
1 | ATGGAACCGG AGATCAAAAT CTGTTTCAAA TACTACCATG GCATTAGCGG AGCCCTGCGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
The HECT type ubiquitin ligase NEDL2 is degraded by anaphase-promoting complex/cyclosome (APC/C)-Cdh1, and its tight regulation maintains the metaphase to anaphase transition. |
