HECW2 cDNA ORF clone, Gallus gallus(chicken)

The following HECW2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECW2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OGa10877 XM_015289488.2
Latest version!
Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa10877 XM_015289489.2
Latest version!
Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
View Old Accession Versions >>
OGa10877 XM_015289489.1 Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OGa10877 XM_015289488.1 Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OGa10877
    Clone ID Related Accession (Same CDS sequence) XM_015289489.1 , XM_015289488.1 , XM_015289489.2 , XM_015289488.2
    Accession Version XM_015289489.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGTT CAGCGCGAGA ACATCTGCTG TTTGTGCGAC GTCGCAACCC ACAGCTTCGG 
    TATACTCTAA GCCCAGAGAA TCTGCAGAGT TTGGCATCTC AGGGCACCAT GGCTGAGAAC
    ATGAATTTGC AACGTGCCAA CAGTGACACT GACTTAGTGA CCTCAGACAG CAGGTCTAGC
    TTGACAGCCA GTATGTATGA GTACACCTTG GGGCAATCTC AGAACCTCAT AATTTTCTGG
    GATATTAAGG AAGAAGTTGA TCCAACTGAC TGGATAGGAC TTTATCACAT AGATGAGAAC
    TCTCCAGCCA ATTTCTGGGA TTCCAAGAAT CGGGGAGTGA CTGGCACACA AAAAGGACAA
    ATTGTATGGC GAATTGAACC TGGTCCCTAT TTTATGGAAT CTGAAATAAA GATCTGTTTT
    AAATACTACC ATGGTGTTAG TGGAGCCCTC CGAGCTACTA CTCCATGTAT CACTGTGAAA
    AACCCTGCTG TGATGATGGG AGTAGAAGGC ACAGATGAAG GTGCTTCTGG AGCCCAGCAC
    TCTCGGAAGT TAGTCAGCTT TACACTATCA GATATTAGAG CAGTTGGACT TAAAAAAGGC
    ATGTTCTTCA ACCCTGATCC TTATCTAAAG ATGTCCATCC AGCCGGGCAA GAAAAGTACA
    TTTCCTACAT TTGCTCATCA TGGTCAGGAA AGAAGATCAA CTATCATCAG TAACACAACT
    AACCCTGTTT GGCACAGAGA GAAATATTCT TTTGTTGCAC TTCTAACAGA TGTCCTGGAA
    ATTGAAGTTA AAGACAAATT TGCCAAGAGT CGTCCAATCA TCAAACGCTT TCTTGGGAAG
    TTAACCATAC CAGTTCAGAG ACTGTTGGAG AGACATGCAA TTGGAGACCA GATTCTCAGC
    TATAATCTTG GCAGAAGGCT CCCAGCGGAT CATGTAAGTG GATATCTCCA GTTCAAAGTG
    GAAATCACAT CTTCTGTTCA TGAAGATGCT TCACCAGAAA CAGTTGGCAC TTTGCTAGGA
    GTGAACTCTG TAAATGGAGA CTTGGGCAGC CCCTCTGACG ATGAGGACAT GCCAACAGGA
    CATCATGATA CTTCCACAGT GTGTTCCAAT GGTCCAATCC TTGAGGAATC TTCTGGAGAA
    ACAATCCAGA GGCATCCTTT AAGGACTAGT ACAACACTAG AAACAGATCA GGATGAGTTA
    ACCTCTGGTG CTTCAAGATC GTCACCTACA AGAGGTCGCC AGGACTCACT CAATGACTAT
    TTGGATGCAA TAGAACACAA TAATCATCCC AGACCTGGGA CGTCTGCCTG TGGCGAGCGT
    CCACGTGGAG CCTCCCCAAA ACTGAGGAGT AGCTTCCCTA CTGACACAAG ACTTAATGCA
    ATGCTTCACA TTGACTCAGA TGAGGAGGAG CATGAGCTAC ATCAAGACCT GGGTTTCCCA
    TCTTCCTTGG AAGATGATGG TGGCTTAGTA ATGCTTAATG GTGCTACAAG AAATGTTGAA
    AGAAATGGTT TAAGTTACTC ATCAGATCAG GTAACACAGG GGCCTGCAGA GGGTGAAAAT
    GTTTCAGCCT CTGAAGCTCC TTTGCCCTCA ACTGATGACC AGACAGAGAG TCTCCCAGAG
    CTTCCACCAT CACAGTTAGT TGAAGGTGAA AGCCTGCAAG GTTCTCAGAG TGAAACACCG
    GCAGACCAGG CTGGCAGTGA CTCATATACT GTGCAGGAGG CAGAGCAGCC TCCCAGTAGT
    ACAGATGCAG GAGCTGCCGA TGCTACTGTT GGAAGCAGAA GGGGTGTTAG TGAAACAGAA
    TCTATTGATC AAGGGTCTGA ACCTTCCCAG CTGTCATCAG AAACTGAGCA GAGTGATCCT
    GCTCGCACAG AAAGTGTCAG TGAAGCTAGC ACCCGGCAAG AAGGGGAGAG TGATGTGGAA
    GGGGCAGACA GCTCTTGCAA TGAAAGTGCA ACCGTGCGAC GTTCCTCAGT TGAAATGCGC
    TGTTCTTTTG AAAGTGCCAG GTTTCCTGAG ACTCCAGCAT TTTCTTCTCA GGAAGAGGAA
    GAGGGAGCTT GTGCCACTGA TGTGGCCAGG ACTGAACCAG TTGTTGAAAC ACAGGACTCT
    CCAAGTACTT CTGGTTCTTT GCCTGCAGTA CAGTTACCTC AGGAGGAAGT ACAGGAGGCT
    GAGGGAGGTG AATTACAAGA CCAAGAGGAT GCAGAAGAAA TCTGGCAAAG AAGAAGTCTG
    CAGGCAGCAG CTGCCAATAG CCAGTCTCAG GATGAAGAAA CGGAAGGAGC CCAAGCAGCT
    TGTGAGGGTG CCACAGCAGA GGTTGAAGGA GCTACTGGAG GTGCTCAAGC CAATGGCCAT
    CAGCCCTTGA GGTCACTACC TTCAGTACGT CAAGATGTTA GCCGGTACCA GCGAGTGGAT
    GAGGCATTAC CACCAAACTG GGAGGCTCGA ATTGACAGCC ATGGACGGAT ATTTTATGTT
    GACCACGTGA ACAGGACAAC AACTTGGCAG CGACCCACAG CCCCTCCAGC CCCACAGATT
    CTCCAGAGAT CAAATTCCAT ACAGCAAATG GAACAGCTGA ATCGCCGGTA TCAGAGCATC
    CGCCGAACAA TGACTAATGA CAGGCCTGAA GAGCAGACAA GTGCAGTGGA TGGAGCTGGA
    GAGGAAACTG ACTTTCACCA TTCTAATGCA GATTTTCGAA GAGAGAATGT GCTGCCTCAC
    TCTACCTCCA GATCCAGACT TACTTTGTTG CTCCAGTCTC CCCCTGTGAA ATTTCTTATC
    AGCCCAGAGT TCTTTACAGT GCTGCATTCT AACCCTAGTG CCTACCGCAT GTTTACAAAT
    AACACGTGTT TGAAGCACAT GATCACCAAA GTCCGGCGGG ACACCCACCA CTTTGAGCGC
    TACCAGCATA ATCGTGATTT GGTGGGCTTC CTAAACATGT TTGCTAACAA ACAGCTGGAG
    CTGCCAAGAG GTTGGGAAAT GAAACATGAC CATCAGGGCA AGGCTTTCTT TGTTGACCAC
    AATTCCCGCA CGACCACGTT CATTGACCCT CGGCTTCCCT TGCAGAGCAG CAGGCCTACC
    AGTGCTTTAG TCCATCGGCA GCACTTAACT AGGCAACGCA GCCACAGTGC TGGTGAGGTT
    GGAGAGGACT CTCGTCATGC AGGACCACCA GTTCTACCAA GACCGTCTAG TACATTTAAT
    ACAGTCAGCA GAGCTCAGTA CCAGGATGTG GTTCCAGTGG CTTACAATGA CAAGATTGTT
    GCATTTTTGC GGCAGCCCAA CATCTTTGAA ATTCTACAAG AGCGTCAACC AGATCTTACC
    AGGAATCATT CACTCAGGGA GAAGATCCAG TTCATCCGGA CAGAGGGAAC GCCTGGCTTG
    GTGCGTTTAT CCAGTGATGC AGACCTTGTC ATGTTACTCA GCTTGTTCGA AGAGGAGATC
    ATGTCATACG TGCCGCCACA TGCCTTACTT CATCCCAGTT ATTGCCAGTC CCCAAGAGGT
    TCGCCAGTGT CCTCACCCCA GAACTCTCCA GGTACTCAGC GTGCCAATGC CCGAGCTCCA
    GCTCCTTACA AAAGAGATTT TGAAGCCAAG CTGAGAAACT TTTACAGGAA GTTGGAAACT
    AAAGGATATG GACAAGGCCC AGGGAAATTG AAATTAATAA TCAGGAGAGA TCACTTGCTT
    GAAGATGCCT TTAACCAGAT TATGGGTTAT TCAAGAAAAG ACCTGCAGAG AAATAAACTC
    TATGTCACAT TTGTTGGGGA AGAGGGGTTG GACTACAGTG GACCTTCAAG GGAATTTTTT
    TTCCTGGTGT CTAGAGAGCT TTTCAATCCG TATTATGGTT TATTTGAATA TTCAGCCAAC
    GATACCTATA CAGTACAAAT AAGTCCCATG TCTGCTTTTG TAGACAATCA CCATGAATGG
    TTCAGGTTTA GCGGTCGAAT CCTTGGTCTT GCTCTGATAC ACCAATATTT GCTAGATGCC
    TTCTTTACAC GACCCTTTTA TAAAGCCCTC CTCCGAATAC TCTGTGATCT GAGTGACCTG
    GAATATCTTG ATGAGGAGTT TCACCAGAGT TTGCAGTGGA TGAAAGATAA TGATATACAT
    GATATCCTGG ATCTCACATT TACTGTAAAT GAAGAAGTTT TTGGGCAGAT CACAGAACGG
    GAATTAAAGC CAGGGGGTGC CAACATTCCA GTCACAGAAA AAAACAAGAA AGAATATATA
    GAAAGAATGG TAAAATGGAG AATTGAGAGA GGAGTTGTAC AACAGACAGA AAGTCTAGTT
    CGTGGCTTCT ATGAGGTGGT TGATGCAAGA CTTGTGTCTG TATTTGATGC GAGAGAACTG
    GAACTAGTTA TAGCTGGAAC AGCAGAAATT GACTTGAGTG ACTGGAGAAA TAATACAGAA
    TATAGAGGAG GTTATCATGA CAATCACATT GTAATTCGGT GGTTCTGGGC TGCTGTGGAA
    AGATTCAACA ATGAACAACG ACTAAGACTT CTGCAGTTTG TTACAGGCAC ATCCAGCATA
    CCTTATGAGG GGTTTGCCTC TCTAAGAGGG AGCAATGGGC CAAGAAGGTT CTGCGTAGAG
    AAGTGGGGGA AGATCACTGC CCTTCCAAGG GCTCACACTT GTTTCAATCG TCTGGACCTT
    CCCCCTTATC CATCATTCTC TATGCTGTAT GAAAAACTGT TGACAGCAGT AGAAGAGACA
    AGTACTTTTG GACTTGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144975.1
    CDS2680..7380
    Translation

    Target ORF information:

    RefSeq Version XM_015289489.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289489.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGTT CAGCGCGAGA ACATCTGCTG TTTGTGCGAC GTCGCAACCC ACAGCTTCGG 
    TATACTCTAA GCCCAGAGAA TCTGCAGAGT TTGGCATCTC AGGGCACCAT GGCTGAGAAC
    ATGAATTTGC AACGTGCCAA CAGTGACACT GACTTAGTGA CCTCAGACAG CAGGTCTAGC
    TTGACAGCCA GTATGTATGA GTACACCTTG GGGCAATCTC AGAACCTCAT AATTTTCTGG
    GATATTAAGG AAGAAGTTGA TCCAACTGAC TGGATAGGAC TTTATCACAT AGATGAGAAC
    TCTCCAGCCA ATTTCTGGGA TTCCAAGAAT CGGGGAGTGA CTGGCACACA AAAAGGACAA
    ATTGTATGGC GAATTGAACC TGGTCCCTAT TTTATGGAAT CTGAAATAAA GATCTGTTTT
    AAATACTACC ATGGTGTTAG TGGAGCCCTC CGAGCTACTA CTCCATGTAT CACTGTGAAA
    AACCCTGCTG TGATGATGGG AGTAGAAGGC ACAGATGAAG GTGCTTCTGG AGCCCAGCAC
    TCTCGGAAGT TAGTCAGCTT TACACTATCA GATATTAGAG CAGTTGGACT TAAAAAAGGC
    ATGTTCTTCA ACCCTGATCC TTATCTAAAG ATGTCCATCC AGCCGGGCAA GAAAAGTACA
    TTTCCTACAT TTGCTCATCA TGGTCAGGAA AGAAGATCAA CTATCATCAG TAACACAACT
    AACCCTGTTT GGCACAGAGA GAAATATTCT TTTGTTGCAC TTCTAACAGA TGTCCTGGAA
    ATTGAAGTTA AAGACAAATT TGCCAAGAGT CGTCCAATCA TCAAACGCTT TCTTGGGAAG
    TTAACCATAC CAGTTCAGAG ACTGTTGGAG AGACATGCAA TTGGAGACCA GATTCTCAGC
    TATAATCTTG GCAGAAGGCT CCCAGCGGAT CATGTAAGTG GATATCTCCA GTTCAAAGTG
    GAAATCACAT CTTCTGTTCA TGAAGATGCT TCACCAGAAA CAGTTGGCAC TTTGCTAGGA
    GTGAACTCTG TAAATGGAGA CTTGGGCAGC CCCTCTGACG ATGAGGACAT GCCAACAGGA
    CATCATGATA CTTCCACAGT GTGTTCCAAT GGTCCAATCC TTGAGGAATC TTCTGGAGAA
    ACAATCCAGA GGCATCCTTT AAGGACTAGT ACAACACTAG AAACAGATCA GGATGAGTTA
    ACCTCTGGTG CTTCAAGATC GTCACCTACA AGAGGTCGCC AGGACTCACT CAATGACTAT
    TTGGATGCAA TAGAACACAA TAATCATCCC AGACCTGGGA CGTCTGCCTG TGGCGAGCGT
    CCACGTGGAG CCTCCCCAAA ACTGAGGAGT AGCTTCCCTA CTGACACAAG ACTTAATGCA
    ATGCTTCACA TTGACTCAGA TGAGGAGGAG CATGAGCTAC ATCAAGACCT GGGTTTCCCA
    TCTTCCTTGG AAGATGATGG TGGCTTAGTA ATGCTTAATG GTGCTACAAG AAATGTTGAA
    AGAAATGGTT TAAGTTACTC ATCAGATCAG GTAACACAGG GGCCTGCAGA GGGTGAAAAT
    GTTTCAGCCT CTGAAGCTCC TTTGCCCTCA ACTGATGACC AGACAGAGAG TCTCCCAGAG
    CTTCCACCAT CACAGTTAGT TGAAGGTGAA AGCCTGCAAG GTTCTCAGAG TGAAACACCG
    GCAGACCAGG CTGGCAGTGA CTCATATACT GTGCAGGAGG CAGAGCAGCC TCCCAGTAGT
    ACAGATGCAG GAGCTGCCGA TGCTACTGTT GGAAGCAGAA GGGGTGTTAG TGAAACAGAA
    TCTATTGATC AAGGGTCTGA ACCTTCCCAG CTGTCATCAG AAACTGAGCA GAGTGATCCT
    GCTCGCACAG AAAGTGTCAG TGAAGCTAGC ACCCGGCAAG AAGGGGAGAG TGATGTGGAA
    GGGGCAGACA GCTCTTGCAA TGAAAGTGCA ACCGTGCGAC GTTCCTCAGT TGAAATGCGC
    TGTTCTTTTG AAAGTGCCAG GTTTCCTGAG ACTCCAGCAT TTTCTTCTCA GGAAGAGGAA
    GAGGGAGCTT GTGCCACTGA TGTGGCCAGG ACTGAACCAG TTGTTGAAAC ACAGGACTCT
    CCAAGTACTT CTGGTTCTTT GCCTGCAGTA CAGTTACCTC AGGAGGAAGT ACAGGAGGCT
    GAGGGAGGTG AATTACAAGA CCAAGAGGAT GCAGAAGAAA TCTGGCAAAG AAGAAGTCTG
    CAGGCAGCAG CTGCCAATAG CCAGTCTCAG GATGAAGAAA CGGAAGGAGC CCAAGCAGCT
    TGTGAGGGTG CCACAGCAGA GGTTGAAGGA GCTACTGGAG GTGCTCAAGC CAATGGCCAT
    CAGCCCTTGA GGTCACTACC TTCAGTACGT CAAGATGTTA GCCGGTACCA GCGAGTGGAT
    GAGGCATTAC CACCAAACTG GGAGGCTCGA ATTGACAGCC ATGGACGGAT ATTTTATGTT
    GACCACGTGA ACAGGACAAC AACTTGGCAG CGACCCACAG CCCCTCCAGC CCCACAGATT
    CTCCAGAGAT CAAATTCCAT ACAGCAAATG GAACAGCTGA ATCGCCGGTA TCAGAGCATC
    CGCCGAACAA TGACTAATGA CAGGCCTGAA GAGCAGACAA GTGCAGTGGA TGGAGCTGGA
    GAGGAAACTG ACTTTCACCA TTCTAATGCA GATTTTCGAA GAGAGAATGT GCTGCCTCAC
    TCTACCTCCA GATCCAGACT TACTTTGTTG CTCCAGTCTC CCCCTGTGAA ATTTCTTATC
    AGCCCAGAGT TCTTTACAGT GCTGCATTCT AACCCTAGTG CCTACCGCAT GTTTACAAAT
    AACACGTGTT TGAAGCACAT GATCACCAAA GTCCGGCGGG ACACCCACCA CTTTGAGCGC
    TACCAGCATA ATCGTGATTT GGTGGGCTTC CTAAACATGT TTGCTAACAA ACAGCTGGAG
    CTGCCAAGAG GTTGGGAAAT GAAACATGAC CATCAGGGCA AGGCTTTCTT TGTTGACCAC
    AATTCCCGCA CGACCACGTT CATTGACCCT CGGCTTCCCT TGCAGAGCAG CAGGCCTACC
    AGTGCTTTAG TCCATCGGCA GCACTTAACT AGGCAACGCA GCCACAGTGC TGGTGAGGTT
    GGAGAGGACT CTCGTCATGC AGGACCACCA GTTCTACCAA GACCGTCTAG TACATTTAAT
    ACAGTCAGCA GAGCTCAGTA CCAGGATGTG GTTCCAGTGG CTTACAATGA CAAGATTGTT
    GCATTTTTGC GGCAGCCCAA CATCTTTGAA ATTCTACAAG AGCGTCAACC AGATCTTACC
    AGGAATCATT CACTCAGGGA GAAGATCCAG TTCATCCGGA CAGAGGGAAC GCCTGGCTTG
    GTGCGTTTAT CCAGTGATGC AGACCTTGTC ATGTTACTCA GCTTGTTCGA AGAGGAGATC
    ATGTCATACG TGCCGCCACA TGCCTTACTT CATCCCAGTT ATTGCCAGTC CCCAAGAGGT
    TCGCCAGTGT CCTCACCCCA GAACTCTCCA GGTACTCAGC GTGCCAATGC CCGAGCTCCA
    GCTCCTTACA AAAGAGATTT TGAAGCCAAG CTGAGAAACT TTTACAGGAA GTTGGAAACT
    AAAGGATATG GACAAGGCCC AGGGAAATTG AAATTAATAA TCAGGAGAGA TCACTTGCTT
    GAAGATGCCT TTAACCAGAT TATGGGTTAT TCAAGAAAAG ACCTGCAGAG AAATAAACTC
    TATGTCACAT TTGTTGGGGA AGAGGGGTTG GACTACAGTG GACCTTCAAG GGAATTTTTT
    TTCCTGGTGT CTAGAGAGCT TTTCAATCCG TATTATGGTT TATTTGAATA TTCAGCCAAC
    GATACCTATA CAGTACAAAT AAGTCCCATG TCTGCTTTTG TAGACAATCA CCATGAATGG
    TTCAGGTTTA GCGGTCGAAT CCTTGGTCTT GCTCTGATAC ACCAATATTT GCTAGATGCC
    TTCTTTACAC GACCCTTTTA TAAAGCCCTC CTCCGAATAC TCTGTGATCT GAGTGACCTG
    GAATATCTTG ATGAGGAGTT TCACCAGAGT TTGCAGTGGA TGAAAGATAA TGATATACAT
    GATATCCTGG ATCTCACATT TACTGTAAAT GAAGAAGTTT TTGGGCAGAT CACAGAACGG
    GAATTAAAGC CAGGGGGTGC CAACATTCCA GTCACAGAAA AAAACAAGAA AGAATATATA
    GAAAGAATGG TAAAATGGAG AATTGAGAGA GGAGTTGTAC AACAGACAGA AAGTCTAGTT
    CGTGGCTTCT ATGAGGTGGT TGATGCAAGA CTTGTGTCTG TATTTGATGC GAGAGAACTG
    GAACTAGTTA TAGCTGGAAC AGCAGAAATT GACTTGAGTG ACTGGAGAAA TAATACAGAA
    TATAGAGGAG GTTATCATGA CAATCACATT GTAATTCGGT GGTTCTGGGC TGCTGTGGAA
    AGATTCAACA ATGAACAACG ACTAAGACTT CTGCAGTTTG TTACAGGCAC ATCCAGCATA
    CCTTATGAGG GGTTTGCCTC TCTAAGAGGG AGCAATGGGC CAAGAAGGTT CTGCGTAGAG
    AAGTGGGGGA AGATCACTGC CCTTCCAAGG GCTCACACTT GTTTCAATCG TCTGGACCTT
    CCCCCTTATC CATCATTCTC TATGCTGTAT GAAAAACTGT TGACAGCAGT AGAAGAGACA
    AGTACTTTTG GACTTGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10877
    Clone ID Related Accession (Same CDS sequence) XM_015289489.1 , XM_015289488.1 , XM_015289489.2 , XM_015289488.2
    Accession Version XM_015289488.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-03
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455902.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGTT CAGCGCGAGA ACATCTGCTG TTTGTGCGAC GTCGCAACCC ACAGCTTCGG 
    TATACTCTAA GCCCAGAGAA TCTGCAGAGT TTGGCATCTC AGGGCACCAT GGCTGAGAAC
    ATGAATTTGC AACGTGCCAA CAGTGACACT GACTTAGTGA CCTCAGACAG CAGGTCTAGC
    TTGACAGCCA GTATGTATGA GTACACCTTG GGGCAATCTC AGAACCTCAT AATTTTCTGG
    GATATTAAGG AAGAAGTTGA TCCAACTGAC TGGATAGGAC TTTATCACAT AGATGAGAAC
    TCTCCAGCCA ATTTCTGGGA TTCCAAGAAT CGGGGAGTGA CTGGCACACA AAAAGGACAA
    ATTGTATGGC GAATTGAACC TGGTCCCTAT TTTATGGAAT CTGAAATAAA GATCTGTTTT
    AAATACTACC ATGGTGTTAG TGGAGCCCTC CGAGCTACTA CTCCATGTAT CACTGTGAAA
    AACCCTGCTG TGATGATGGG AGTAGAAGGC ACAGATGAAG GTGCTTCTGG AGCCCAGCAC
    TCTCGGAAGT TAGTCAGCTT TACACTATCA GATATTAGAG CAGTTGGACT TAAAAAAGGC
    ATGTTCTTCA ACCCTGATCC TTATCTAAAG ATGTCCATCC AGCCGGGCAA GAAAAGTACA
    TTTCCTACAT TTGCTCATCA TGGTCAGGAA AGAAGATCAA CTATCATCAG TAACACAACT
    AACCCTGTTT GGCACAGAGA GAAATATTCT TTTGTTGCAC TTCTAACAGA TGTCCTGGAA
    ATTGAAGTTA AAGACAAATT TGCCAAGAGT CGTCCAATCA TCAAACGCTT TCTTGGGAAG
    TTAACCATAC CAGTTCAGAG ACTGTTGGAG AGACATGCAA TTGGAGACCA GATTCTCAGC
    TATAATCTTG GCAGAAGGCT CCCAGCGGAT CATGTAAGTG GATATCTCCA GTTCAAAGTG
    GAAATCACAT CTTCTGTTCA TGAAGATGCT TCACCAGAAA CAGTTGGCAC TTTGCTAGGA
    GTGAACTCTG TAAATGGAGA CTTGGGCAGC CCCTCTGACG ATGAGGACAT GCCAACAGGA
    CATCATGATA CTTCCACAGT GTGTTCCAAT GGTCCAATCC TTGAGGAATC TTCTGGAGAA
    ACAATCCAGA GGCATCCTTT AAGGACTAGT ACAACACTAG AAACAGATCA GGATGAGTTA
    ACCTCTGGTG CTTCAAGATC GTCACCTACA AGAGGTCGCC AGGACTCACT CAATGACTAT
    TTGGATGCAA TAGAACACAA TAATCATCCC AGACCTGGGA CGTCTGCCTG TGGCGAGCGT
    CCACGTGGAG CCTCCCCAAA ACTGAGGAGT AGCTTCCCTA CTGACACAAG ACTTAATGCA
    ATGCTTCACA TTGACTCAGA TGAGGAGGAG CATGAGCTAC ATCAAGACCT GGGTTTCCCA
    TCTTCCTTGG AAGATGATGG TGGCTTAGTA ATGCTTAATG GTGCTACAAG AAATGTTGAA
    AGAAATGGTT TAAGTTACTC ATCAGATCAG GTAACACAGG GGCCTGCAGA GGGTGAAAAT
    GTTTCAGCCT CTGAAGCTCC TTTGCCCTCA ACTGATGACC AGACAGAGAG TCTCCCAGAG
    CTTCCACCAT CACAGTTAGT TGAAGGTGAA AGCCTGCAAG GTTCTCAGAG TGAAACACCG
    GCAGACCAGG CTGGCAGTGA CTCATATACT GTGCAGGAGG CAGAGCAGCC TCCCAGTAGT
    ACAGATGCAG GAGCTGCCGA TGCTACTGTT GGAAGCAGAA GGGGTGTTAG TGAAACAGAA
    TCTATTGATC AAGGGTCTGA ACCTTCCCAG CTGTCATCAG AAACTGAGCA GAGTGATCCT
    GCTCGCACAG AAAGTGTCAG TGAAGCTAGC ACCCGGCAAG AAGGGGAGAG TGATGTGGAA
    GGGGCAGACA GCTCTTGCAA TGAAAGTGCA ACCGTGCGAC GTTCCTCAGT TGAAATGCGC
    TGTTCTTTTG AAAGTGCCAG GTTTCCTGAG ACTCCAGCAT TTTCTTCTCA GGAAGAGGAA
    GAGGGAGCTT GTGCCACTGA TGTGGCCAGG ACTGAACCAG TTGTTGAAAC ACAGGACTCT
    CCAAGTACTT CTGGTTCTTT GCCTGCAGTA CAGTTACCTC AGGAGGAAGT ACAGGAGGCT
    GAGGGAGGTG AATTACAAGA CCAAGAGGAT GCAGAAGAAA TCTGGCAAAG AAGAAGTCTG
    CAGGCAGCAG CTGCCAATAG CCAGTCTCAG GATGAAGAAA CGGAAGGAGC CCAAGCAGCT
    TGTGAGGGTG CCACAGCAGA GGTTGAAGGA GCTACTGGAG GTGCTCAAGC CAATGGCCAT
    CAGCCCTTGA GGTCACTACC TTCAGTACGT CAAGATGTTA GCCGGTACCA GCGAGTGGAT
    GAGGCATTAC CACCAAACTG GGAGGCTCGA ATTGACAGCC ATGGACGGAT ATTTTATGTT
    GACCACGTGA ACAGGACAAC AACTTGGCAG CGACCCACAG CCCCTCCAGC CCCACAGATT
    CTCCAGAGAT CAAATTCCAT ACAGCAAATG GAACAGCTGA ATCGCCGGTA TCAGAGCATC
    CGCCGAACAA TGACTAATGA CAGGCCTGAA GAGCAGACAA GTGCAGTGGA TGGAGCTGGA
    GAGGAAACTG ACTTTCACCA TTCTAATGCA GATTTTCGAA GAGAGAATGT GCTGCCTCAC
    TCTACCTCCA GATCCAGACT TACTTTGTTG CTCCAGTCTC CCCCTGTGAA ATTTCTTATC
    AGCCCAGAGT TCTTTACAGT GCTGCATTCT AACCCTAGTG CCTACCGCAT GTTTACAAAT
    AACACGTGTT TGAAGCACAT GATCACCAAA GTCCGGCGGG ACACCCACCA CTTTGAGCGC
    TACCAGCATA ATCGTGATTT GGTGGGCTTC CTAAACATGT TTGCTAACAA ACAGCTGGAG
    CTGCCAAGAG GTTGGGAAAT GAAACATGAC CATCAGGGCA AGGCTTTCTT TGTTGACCAC
    AATTCCCGCA CGACCACGTT CATTGACCCT CGGCTTCCCT TGCAGAGCAG CAGGCCTACC
    AGTGCTTTAG TCCATCGGCA GCACTTAACT AGGCAACGCA GCCACAGTGC TGGTGAGGTT
    GGAGAGGACT CTCGTCATGC AGGACCACCA GTTCTACCAA GACCGTCTAG TACATTTAAT
    ACAGTCAGCA GAGCTCAGTA CCAGGATGTG GTTCCAGTGG CTTACAATGA CAAGATTGTT
    GCATTTTTGC GGCAGCCCAA CATCTTTGAA ATTCTACAAG AGCGTCAACC AGATCTTACC
    AGGAATCATT CACTCAGGGA GAAGATCCAG TTCATCCGGA CAGAGGGAAC GCCTGGCTTG
    GTGCGTTTAT CCAGTGATGC AGACCTTGTC ATGTTACTCA GCTTGTTCGA AGAGGAGATC
    ATGTCATACG TGCCGCCACA TGCCTTACTT CATCCCAGTT ATTGCCAGTC CCCAAGAGGT
    TCGCCAGTGT CCTCACCCCA GAACTCTCCA GGTACTCAGC GTGCCAATGC CCGAGCTCCA
    GCTCCTTACA AAAGAGATTT TGAAGCCAAG CTGAGAAACT TTTACAGGAA GTTGGAAACT
    AAAGGATATG GACAAGGCCC AGGGAAATTG AAATTAATAA TCAGGAGAGA TCACTTGCTT
    GAAGATGCCT TTAACCAGAT TATGGGTTAT TCAAGAAAAG ACCTGCAGAG AAATAAACTC
    TATGTCACAT TTGTTGGGGA AGAGGGGTTG GACTACAGTG GACCTTCAAG GGAATTTTTT
    TTCCTGGTGT CTAGAGAGCT TTTCAATCCG TATTATGGTT TATTTGAATA TTCAGCCAAC
    GATACCTATA CAGTACAAAT AAGTCCCATG TCTGCTTTTG TAGACAATCA CCATGAATGG
    TTCAGGTTTA GCGGTCGAAT CCTTGGTCTT GCTCTGATAC ACCAATATTT GCTAGATGCC
    TTCTTTACAC GACCCTTTTA TAAAGCCCTC CTCCGAATAC TCTGTGATCT GAGTGACCTG
    GAATATCTTG ATGAGGAGTT TCACCAGAGT TTGCAGTGGA TGAAAGATAA TGATATACAT
    GATATCCTGG ATCTCACATT TACTGTAAAT GAAGAAGTTT TTGGGCAGAT CACAGAACGG
    GAATTAAAGC CAGGGGGTGC CAACATTCCA GTCACAGAAA AAAACAAGAA AGAATATATA
    GAAAGAATGG TAAAATGGAG AATTGAGAGA GGAGTTGTAC AACAGACAGA AAGTCTAGTT
    CGTGGCTTCT ATGAGGTGGT TGATGCAAGA CTTGTGTCTG TATTTGATGC GAGAGAACTG
    GAACTAGTTA TAGCTGGAAC AGCAGAAATT GACTTGAGTG ACTGGAGAAA TAATACAGAA
    TATAGAGGAG GTTATCATGA CAATCACATT GTAATTCGGT GGTTCTGGGC TGCTGTGGAA
    AGATTCAACA ATGAACAACG ACTAAGACTT CTGCAGTTTG TTACAGGCAC ATCCAGCATA
    CCTTATGAGG GGTTTGCCTC TCTAAGAGGG AGCAATGGGC CAAGAAGGTT CTGCGTAGAG
    AAGTGGGGGA AGATCACTGC CCTTCCAAGG GCTCACACTT GTTTCAATCG TCTGGACCTT
    CCCCCTTATC CATCATTCTC TATGCTGTAT GAAAAACTGT TGACAGCAGT AGAAGAGACA
    AGTACTTTTG GACTTGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144974.1
    CDS185..4885
    Translation

    Target ORF information:

    RefSeq Version XM_015289488.1
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289488.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGTT CAGCGCGAGA ACATCTGCTG TTTGTGCGAC GTCGCAACCC ACAGCTTCGG 
    TATACTCTAA GCCCAGAGAA TCTGCAGAGT TTGGCATCTC AGGGCACCAT GGCTGAGAAC
    ATGAATTTGC AACGTGCCAA CAGTGACACT GACTTAGTGA CCTCAGACAG CAGGTCTAGC
    TTGACAGCCA GTATGTATGA GTACACCTTG GGGCAATCTC AGAACCTCAT AATTTTCTGG
    GATATTAAGG AAGAAGTTGA TCCAACTGAC TGGATAGGAC TTTATCACAT AGATGAGAAC
    TCTCCAGCCA ATTTCTGGGA TTCCAAGAAT CGGGGAGTGA CTGGCACACA AAAAGGACAA
    ATTGTATGGC GAATTGAACC TGGTCCCTAT TTTATGGAAT CTGAAATAAA GATCTGTTTT
    AAATACTACC ATGGTGTTAG TGGAGCCCTC CGAGCTACTA CTCCATGTAT CACTGTGAAA
    AACCCTGCTG TGATGATGGG AGTAGAAGGC ACAGATGAAG GTGCTTCTGG AGCCCAGCAC
    TCTCGGAAGT TAGTCAGCTT TACACTATCA GATATTAGAG CAGTTGGACT TAAAAAAGGC
    ATGTTCTTCA ACCCTGATCC TTATCTAAAG ATGTCCATCC AGCCGGGCAA GAAAAGTACA
    TTTCCTACAT TTGCTCATCA TGGTCAGGAA AGAAGATCAA CTATCATCAG TAACACAACT
    AACCCTGTTT GGCACAGAGA GAAATATTCT TTTGTTGCAC TTCTAACAGA TGTCCTGGAA
    ATTGAAGTTA AAGACAAATT TGCCAAGAGT CGTCCAATCA TCAAACGCTT TCTTGGGAAG
    TTAACCATAC CAGTTCAGAG ACTGTTGGAG AGACATGCAA TTGGAGACCA GATTCTCAGC
    TATAATCTTG GCAGAAGGCT CCCAGCGGAT CATGTAAGTG GATATCTCCA GTTCAAAGTG
    GAAATCACAT CTTCTGTTCA TGAAGATGCT TCACCAGAAA CAGTTGGCAC TTTGCTAGGA
    GTGAACTCTG TAAATGGAGA CTTGGGCAGC CCCTCTGACG ATGAGGACAT GCCAACAGGA
    CATCATGATA CTTCCACAGT GTGTTCCAAT GGTCCAATCC TTGAGGAATC TTCTGGAGAA
    ACAATCCAGA GGCATCCTTT AAGGACTAGT ACAACACTAG AAACAGATCA GGATGAGTTA
    ACCTCTGGTG CTTCAAGATC GTCACCTACA AGAGGTCGCC AGGACTCACT CAATGACTAT
    TTGGATGCAA TAGAACACAA TAATCATCCC AGACCTGGGA CGTCTGCCTG TGGCGAGCGT
    CCACGTGGAG CCTCCCCAAA ACTGAGGAGT AGCTTCCCTA CTGACACAAG ACTTAATGCA
    ATGCTTCACA TTGACTCAGA TGAGGAGGAG CATGAGCTAC ATCAAGACCT GGGTTTCCCA
    TCTTCCTTGG AAGATGATGG TGGCTTAGTA ATGCTTAATG GTGCTACAAG AAATGTTGAA
    AGAAATGGTT TAAGTTACTC ATCAGATCAG GTAACACAGG GGCCTGCAGA GGGTGAAAAT
    GTTTCAGCCT CTGAAGCTCC TTTGCCCTCA ACTGATGACC AGACAGAGAG TCTCCCAGAG
    CTTCCACCAT CACAGTTAGT TGAAGGTGAA AGCCTGCAAG GTTCTCAGAG TGAAACACCG
    GCAGACCAGG CTGGCAGTGA CTCATATACT GTGCAGGAGG CAGAGCAGCC TCCCAGTAGT
    ACAGATGCAG GAGCTGCCGA TGCTACTGTT GGAAGCAGAA GGGGTGTTAG TGAAACAGAA
    TCTATTGATC AAGGGTCTGA ACCTTCCCAG CTGTCATCAG AAACTGAGCA GAGTGATCCT
    GCTCGCACAG AAAGTGTCAG TGAAGCTAGC ACCCGGCAAG AAGGGGAGAG TGATGTGGAA
    GGGGCAGACA GCTCTTGCAA TGAAAGTGCA ACCGTGCGAC GTTCCTCAGT TGAAATGCGC
    TGTTCTTTTG AAAGTGCCAG GTTTCCTGAG ACTCCAGCAT TTTCTTCTCA GGAAGAGGAA
    GAGGGAGCTT GTGCCACTGA TGTGGCCAGG ACTGAACCAG TTGTTGAAAC ACAGGACTCT
    CCAAGTACTT CTGGTTCTTT GCCTGCAGTA CAGTTACCTC AGGAGGAAGT ACAGGAGGCT
    GAGGGAGGTG AATTACAAGA CCAAGAGGAT GCAGAAGAAA TCTGGCAAAG AAGAAGTCTG
    CAGGCAGCAG CTGCCAATAG CCAGTCTCAG GATGAAGAAA CGGAAGGAGC CCAAGCAGCT
    TGTGAGGGTG CCACAGCAGA GGTTGAAGGA GCTACTGGAG GTGCTCAAGC CAATGGCCAT
    CAGCCCTTGA GGTCACTACC TTCAGTACGT CAAGATGTTA GCCGGTACCA GCGAGTGGAT
    GAGGCATTAC CACCAAACTG GGAGGCTCGA ATTGACAGCC ATGGACGGAT ATTTTATGTT
    GACCACGTGA ACAGGACAAC AACTTGGCAG CGACCCACAG CCCCTCCAGC CCCACAGATT
    CTCCAGAGAT CAAATTCCAT ACAGCAAATG GAACAGCTGA ATCGCCGGTA TCAGAGCATC
    CGCCGAACAA TGACTAATGA CAGGCCTGAA GAGCAGACAA GTGCAGTGGA TGGAGCTGGA
    GAGGAAACTG ACTTTCACCA TTCTAATGCA GATTTTCGAA GAGAGAATGT GCTGCCTCAC
    TCTACCTCCA GATCCAGACT TACTTTGTTG CTCCAGTCTC CCCCTGTGAA ATTTCTTATC
    AGCCCAGAGT TCTTTACAGT GCTGCATTCT AACCCTAGTG CCTACCGCAT GTTTACAAAT
    AACACGTGTT TGAAGCACAT GATCACCAAA GTCCGGCGGG ACACCCACCA CTTTGAGCGC
    TACCAGCATA ATCGTGATTT GGTGGGCTTC CTAAACATGT TTGCTAACAA ACAGCTGGAG
    CTGCCAAGAG GTTGGGAAAT GAAACATGAC CATCAGGGCA AGGCTTTCTT TGTTGACCAC
    AATTCCCGCA CGACCACGTT CATTGACCCT CGGCTTCCCT TGCAGAGCAG CAGGCCTACC
    AGTGCTTTAG TCCATCGGCA GCACTTAACT AGGCAACGCA GCCACAGTGC TGGTGAGGTT
    GGAGAGGACT CTCGTCATGC AGGACCACCA GTTCTACCAA GACCGTCTAG TACATTTAAT
    ACAGTCAGCA GAGCTCAGTA CCAGGATGTG GTTCCAGTGG CTTACAATGA CAAGATTGTT
    GCATTTTTGC GGCAGCCCAA CATCTTTGAA ATTCTACAAG AGCGTCAACC AGATCTTACC
    AGGAATCATT CACTCAGGGA GAAGATCCAG TTCATCCGGA CAGAGGGAAC GCCTGGCTTG
    GTGCGTTTAT CCAGTGATGC AGACCTTGTC ATGTTACTCA GCTTGTTCGA AGAGGAGATC
    ATGTCATACG TGCCGCCACA TGCCTTACTT CATCCCAGTT ATTGCCAGTC CCCAAGAGGT
    TCGCCAGTGT CCTCACCCCA GAACTCTCCA GGTACTCAGC GTGCCAATGC CCGAGCTCCA
    GCTCCTTACA AAAGAGATTT TGAAGCCAAG CTGAGAAACT TTTACAGGAA GTTGGAAACT
    AAAGGATATG GACAAGGCCC AGGGAAATTG AAATTAATAA TCAGGAGAGA TCACTTGCTT
    GAAGATGCCT TTAACCAGAT TATGGGTTAT TCAAGAAAAG ACCTGCAGAG AAATAAACTC
    TATGTCACAT TTGTTGGGGA AGAGGGGTTG GACTACAGTG GACCTTCAAG GGAATTTTTT
    TTCCTGGTGT CTAGAGAGCT TTTCAATCCG TATTATGGTT TATTTGAATA TTCAGCCAAC
    GATACCTATA CAGTACAAAT AAGTCCCATG TCTGCTTTTG TAGACAATCA CCATGAATGG
    TTCAGGTTTA GCGGTCGAAT CCTTGGTCTT GCTCTGATAC ACCAATATTT GCTAGATGCC
    TTCTTTACAC GACCCTTTTA TAAAGCCCTC CTCCGAATAC TCTGTGATCT GAGTGACCTG
    GAATATCTTG ATGAGGAGTT TCACCAGAGT TTGCAGTGGA TGAAAGATAA TGATATACAT
    GATATCCTGG ATCTCACATT TACTGTAAAT GAAGAAGTTT TTGGGCAGAT CACAGAACGG
    GAATTAAAGC CAGGGGGTGC CAACATTCCA GTCACAGAAA AAAACAAGAA AGAATATATA
    GAAAGAATGG TAAAATGGAG AATTGAGAGA GGAGTTGTAC AACAGACAGA AAGTCTAGTT
    CGTGGCTTCT ATGAGGTGGT TGATGCAAGA CTTGTGTCTG TATTTGATGC GAGAGAACTG
    GAACTAGTTA TAGCTGGAAC AGCAGAAATT GACTTGAGTG ACTGGAGAAA TAATACAGAA
    TATAGAGGAG GTTATCATGA CAATCACATT GTAATTCGGT GGTTCTGGGC TGCTGTGGAA
    AGATTCAACA ATGAACAACG ACTAAGACTT CTGCAGTTTG TTACAGGCAC ATCCAGCATA
    CCTTATGAGG GGTTTGCCTC TCTAAGAGGG AGCAATGGGC CAAGAAGGTT CTGCGTAGAG
    AAGTGGGGGA AGATCACTGC CCTTCCAAGG GCTCACACTT GTTTCAATCG TCTGGACCTT
    CCCCCTTATC CATCATTCTC TATGCTGTAT GAAAAACTGT TGACAGCAGT AGAAGAGACA
    AGTACTTTTG GACTTGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10877
    Clone ID Related Accession (Same CDS sequence) XM_015289489.1 , XM_015289488.1 , XM_015289489.2 , XM_015289488.2
    Accession Version XM_015289489.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289489.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGTT CAGCGCGAGA ACATCTGCTG TTTGTGCGAC GTCGCAACCC ACAGCTTCGG 
    TATACTCTAA GCCCAGAGAA TCTGCAGAGT TTGGCATCTC AGGGCACCAT GGCTGAGAAC
    ATGAATTTGC AACGTGCCAA CAGTGACACT GACTTAGTGA CCTCAGACAG CAGGTCTAGC
    TTGACAGCCA GTATGTATGA GTACACCTTG GGGCAATCTC AGAACCTCAT AATTTTCTGG
    GATATTAAGG AAGAAGTTGA TCCAACTGAC TGGATAGGAC TTTATCACAT AGATGAGAAC
    TCTCCAGCCA ATTTCTGGGA TTCCAAGAAT CGGGGAGTGA CTGGCACACA AAAAGGACAA
    ATTGTATGGC GAATTGAACC TGGTCCCTAT TTTATGGAAT CTGAAATAAA GATCTGTTTT
    AAATACTACC ATGGTGTTAG TGGAGCCCTC CGAGCTACTA CTCCATGTAT CACTGTGAAA
    AACCCTGCTG TGATGATGGG AGTAGAAGGC ACAGATGAAG GTGCTTCTGG AGCCCAGCAC
    TCTCGGAAGT TAGTCAGCTT TACACTATCA GATATTAGAG CAGTTGGACT TAAAAAAGGC
    ATGTTCTTCA ACCCTGATCC TTATCTAAAG ATGTCCATCC AGCCGGGCAA GAAAAGTACA
    TTTCCTACAT TTGCTCATCA TGGTCAGGAA AGAAGATCAA CTATCATCAG TAACACAACT
    AACCCTGTTT GGCACAGAGA GAAATATTCT TTTGTTGCAC TTCTAACAGA TGTCCTGGAA
    ATTGAAGTTA AAGACAAATT TGCCAAGAGT CGTCCAATCA TCAAACGCTT TCTTGGGAAG
    TTAACCATAC CAGTTCAGAG ACTGTTGGAG AGACATGCAA TTGGAGACCA GATTCTCAGC
    TATAATCTTG GCAGAAGGCT CCCAGCGGAT CATGTAAGTG GATATCTCCA GTTCAAAGTG
    GAAATCACAT CTTCTGTTCA TGAAGATGCT TCACCAGAAA CAGTTGGCAC TTTGCTAGGA
    GTGAACTCTG TAAATGGAGA CTTGGGCAGC CCCTCTGACG ATGAGGACAT GCCAACAGGA
    CATCATGATA CTTCCACAGT GTGTTCCAAT GGTCCAATCC TTGAGGAATC TTCTGGAGAA
    ACAATCCAGA GGCATCCTTT AAGGACTAGT ACAACACTAG AAACAGATCA GGATGAGTTA
    ACCTCTGGTG CTTCAAGATC GTCACCTACA AGAGGTCGCC AGGACTCACT CAATGACTAT
    TTGGATGCAA TAGAACACAA TAATCATCCC AGACCTGGGA CGTCTGCCTG TGGCGAGCGT
    CCACGTGGAG CCTCCCCAAA ACTGAGGAGT AGCTTCCCTA CTGACACAAG ACTTAATGCA
    ATGCTTCACA TTGACTCAGA TGAGGAGGAG CATGAGCTAC ATCAAGACCT GGGTTTCCCA
    TCTTCCTTGG AAGATGATGG TGGCTTAGTA ATGCTTAATG GTGCTACAAG AAATGTTGAA
    AGAAATGGTT TAAGTTACTC ATCAGATCAG GTAACACAGG GGCCTGCAGA GGGTGAAAAT
    GTTTCAGCCT CTGAAGCTCC TTTGCCCTCA ACTGATGACC AGACAGAGAG TCTCCCAGAG
    CTTCCACCAT CACAGTTAGT TGAAGGTGAA AGCCTGCAAG GTTCTCAGAG TGAAACACCG
    GCAGACCAGG CTGGCAGTGA CTCATATACT GTGCAGGAGG CAGAGCAGCC TCCCAGTAGT
    ACAGATGCAG GAGCTGCCGA TGCTACTGTT GGAAGCAGAA GGGGTGTTAG TGAAACAGAA
    TCTATTGATC AAGGGTCTGA ACCTTCCCAG CTGTCATCAG AAACTGAGCA GAGTGATCCT
    GCTCGCACAG AAAGTGTCAG TGAAGCTAGC ACCCGGCAAG AAGGGGAGAG TGATGTGGAA
    GGGGCAGACA GCTCTTGCAA TGAAAGTGCA ACCGTGCGAC GTTCCTCAGT TGAAATGCGC
    TGTTCTTTTG AAAGTGCCAG GTTTCCTGAG ACTCCAGCAT TTTCTTCTCA GGAAGAGGAA
    GAGGGAGCTT GTGCCACTGA TGTGGCCAGG ACTGAACCAG TTGTTGAAAC ACAGGACTCT
    CCAAGTACTT CTGGTTCTTT GCCTGCAGTA CAGTTACCTC AGGAGGAAGT ACAGGAGGCT
    GAGGGAGGTG AATTACAAGA CCAAGAGGAT GCAGAAGAAA TCTGGCAAAG AAGAAGTCTG
    CAGGCAGCAG CTGCCAATAG CCAGTCTCAG GATGAAGAAA CGGAAGGAGC CCAAGCAGCT
    TGTGAGGGTG CCACAGCAGA GGTTGAAGGA GCTACTGGAG GTGCTCAAGC CAATGGCCAT
    CAGCCCTTGA GGTCACTACC TTCAGTACGT CAAGATGTTA GCCGGTACCA GCGAGTGGAT
    GAGGCATTAC CACCAAACTG GGAGGCTCGA ATTGACAGCC ATGGACGGAT ATTTTATGTT
    GACCACGTGA ACAGGACAAC AACTTGGCAG CGACCCACAG CCCCTCCAGC CCCACAGATT
    CTCCAGAGAT CAAATTCCAT ACAGCAAATG GAACAGCTGA ATCGCCGGTA TCAGAGCATC
    CGCCGAACAA TGACTAATGA CAGGCCTGAA GAGCAGACAA GTGCAGTGGA TGGAGCTGGA
    GAGGAAACTG ACTTTCACCA TTCTAATGCA GATTTTCGAA GAGAGAATGT GCTGCCTCAC
    TCTACCTCCA GATCCAGACT TACTTTGTTG CTCCAGTCTC CCCCTGTGAA ATTTCTTATC
    AGCCCAGAGT TCTTTACAGT GCTGCATTCT AACCCTAGTG CCTACCGCAT GTTTACAAAT
    AACACGTGTT TGAAGCACAT GATCACCAAA GTCCGGCGGG ACACCCACCA CTTTGAGCGC
    TACCAGCATA ATCGTGATTT GGTGGGCTTC CTAAACATGT TTGCTAACAA ACAGCTGGAG
    CTGCCAAGAG GTTGGGAAAT GAAACATGAC CATCAGGGCA AGGCTTTCTT TGTTGACCAC
    AATTCCCGCA CGACCACGTT CATTGACCCT CGGCTTCCCT TGCAGAGCAG CAGGCCTACC
    AGTGCTTTAG TCCATCGGCA GCACTTAACT AGGCAACGCA GCCACAGTGC TGGTGAGGTT
    GGAGAGGACT CTCGTCATGC AGGACCACCA GTTCTACCAA GACCGTCTAG TACATTTAAT
    ACAGTCAGCA GAGCTCAGTA CCAGGATGTG GTTCCAGTGG CTTACAATGA CAAGATTGTT
    GCATTTTTGC GGCAGCCCAA CATCTTTGAA ATTCTACAAG AGCGTCAACC AGATCTTACC
    AGGAATCATT CACTCAGGGA GAAGATCCAG TTCATCCGGA CAGAGGGAAC GCCTGGCTTG
    GTGCGTTTAT CCAGTGATGC AGACCTTGTC ATGTTACTCA GCTTGTTCGA AGAGGAGATC
    ATGTCATACG TGCCGCCACA TGCCTTACTT CATCCCAGTT ATTGCCAGTC CCCAAGAGGT
    TCGCCAGTGT CCTCACCCCA GAACTCTCCA GGTACTCAGC GTGCCAATGC CCGAGCTCCA
    GCTCCTTACA AAAGAGATTT TGAAGCCAAG CTGAGAAACT TTTACAGGAA GTTGGAAACT
    AAAGGATATG GACAAGGCCC AGGGAAATTG AAATTAATAA TCAGGAGAGA TCACTTGCTT
    GAAGATGCCT TTAACCAGAT TATGGGTTAT TCAAGAAAAG ACCTGCAGAG AAATAAACTC
    TATGTCACAT TTGTTGGGGA AGAGGGGTTG GACTACAGTG GACCTTCAAG GGAATTTTTT
    TTCCTGGTGT CTAGAGAGCT TTTCAATCCG TATTATGGTT TATTTGAATA TTCAGCCAAC
    GATACCTATA CAGTACAAAT AAGTCCCATG TCTGCTTTTG TAGACAATCA CCATGAATGG
    TTCAGGTTTA GCGGTCGAAT CCTTGGTCTT GCTCTGATAC ACCAATATTT GCTAGATGCC
    TTCTTTACAC GACCCTTTTA TAAAGCCCTC CTCCGAATAC TCTGTGATCT GAGTGACCTG
    GAATATCTTG ATGAGGAGTT TCACCAGAGT TTGCAGTGGA TGAAAGATAA TGATATACAT
    GATATCCTGG ATCTCACATT TACTGTAAAT GAAGAAGTTT TTGGGCAGAT CACAGAACGG
    GAATTAAAGC CAGGGGGTGC CAACATTCCA GTCACAGAAA AAAACAAGAA AGAATATATA
    GAAAGAATGG TAAAATGGAG AATTGAGAGA GGAGTTGTAC AACAGACAGA AAGTCTAGTT
    CGTGGCTTCT ATGAGGTGGT TGATGCAAGA CTTGTGTCTG TATTTGATGC GAGAGAACTG
    GAACTAGTTA TAGCTGGAAC AGCAGAAATT GACTTGAGTG ACTGGAGAAA TAATACAGAA
    TATAGAGGAG GTTATCATGA CAATCACATT GTAATTCGGT GGTTCTGGGC TGCTGTGGAA
    AGATTCAACA ATGAACAACG ACTAAGACTT CTGCAGTTTG TTACAGGCAC ATCCAGCATA
    CCTTATGAGG GGTTTGCCTC TCTAAGAGGG AGCAATGGGC CAAGAAGGTT CTGCGTAGAG
    AAGTGGGGGA AGATCACTGC CCTTCCAAGG GCTCACACTT GTTTCAATCG TCTGGACCTT
    CCCCCTTATC CATCATTCTC TATGCTGTAT GAAAAACTGT TGACAGCAGT AGAAGAGACA
    AGTACTTTTG GACTTGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144975.1
    CDS2797..7497
    Translation

    Target ORF information:

    RefSeq Version XM_015289489.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289489.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGTT CAGCGCGAGA ACATCTGCTG TTTGTGCGAC GTCGCAACCC ACAGCTTCGG 
    TATACTCTAA GCCCAGAGAA TCTGCAGAGT TTGGCATCTC AGGGCACCAT GGCTGAGAAC
    ATGAATTTGC AACGTGCCAA CAGTGACACT GACTTAGTGA CCTCAGACAG CAGGTCTAGC
    TTGACAGCCA GTATGTATGA GTACACCTTG GGGCAATCTC AGAACCTCAT AATTTTCTGG
    GATATTAAGG AAGAAGTTGA TCCAACTGAC TGGATAGGAC TTTATCACAT AGATGAGAAC
    TCTCCAGCCA ATTTCTGGGA TTCCAAGAAT CGGGGAGTGA CTGGCACACA AAAAGGACAA
    ATTGTATGGC GAATTGAACC TGGTCCCTAT TTTATGGAAT CTGAAATAAA GATCTGTTTT
    AAATACTACC ATGGTGTTAG TGGAGCCCTC CGAGCTACTA CTCCATGTAT CACTGTGAAA
    AACCCTGCTG TGATGATGGG AGTAGAAGGC ACAGATGAAG GTGCTTCTGG AGCCCAGCAC
    TCTCGGAAGT TAGTCAGCTT TACACTATCA GATATTAGAG CAGTTGGACT TAAAAAAGGC
    ATGTTCTTCA ACCCTGATCC TTATCTAAAG ATGTCCATCC AGCCGGGCAA GAAAAGTACA
    TTTCCTACAT TTGCTCATCA TGGTCAGGAA AGAAGATCAA CTATCATCAG TAACACAACT
    AACCCTGTTT GGCACAGAGA GAAATATTCT TTTGTTGCAC TTCTAACAGA TGTCCTGGAA
    ATTGAAGTTA AAGACAAATT TGCCAAGAGT CGTCCAATCA TCAAACGCTT TCTTGGGAAG
    TTAACCATAC CAGTTCAGAG ACTGTTGGAG AGACATGCAA TTGGAGACCA GATTCTCAGC
    TATAATCTTG GCAGAAGGCT CCCAGCGGAT CATGTAAGTG GATATCTCCA GTTCAAAGTG
    GAAATCACAT CTTCTGTTCA TGAAGATGCT TCACCAGAAA CAGTTGGCAC TTTGCTAGGA
    GTGAACTCTG TAAATGGAGA CTTGGGCAGC CCCTCTGACG ATGAGGACAT GCCAACAGGA
    CATCATGATA CTTCCACAGT GTGTTCCAAT GGTCCAATCC TTGAGGAATC TTCTGGAGAA
    ACAATCCAGA GGCATCCTTT AAGGACTAGT ACAACACTAG AAACAGATCA GGATGAGTTA
    ACCTCTGGTG CTTCAAGATC GTCACCTACA AGAGGTCGCC AGGACTCACT CAATGACTAT
    TTGGATGCAA TAGAACACAA TAATCATCCC AGACCTGGGA CGTCTGCCTG TGGCGAGCGT
    CCACGTGGAG CCTCCCCAAA ACTGAGGAGT AGCTTCCCTA CTGACACAAG ACTTAATGCA
    ATGCTTCACA TTGACTCAGA TGAGGAGGAG CATGAGCTAC ATCAAGACCT GGGTTTCCCA
    TCTTCCTTGG AAGATGATGG TGGCTTAGTA ATGCTTAATG GTGCTACAAG AAATGTTGAA
    AGAAATGGTT TAAGTTACTC ATCAGATCAG GTAACACAGG GGCCTGCAGA GGGTGAAAAT
    GTTTCAGCCT CTGAAGCTCC TTTGCCCTCA ACTGATGACC AGACAGAGAG TCTCCCAGAG
    CTTCCACCAT CACAGTTAGT TGAAGGTGAA AGCCTGCAAG GTTCTCAGAG TGAAACACCG
    GCAGACCAGG CTGGCAGTGA CTCATATACT GTGCAGGAGG CAGAGCAGCC TCCCAGTAGT
    ACAGATGCAG GAGCTGCCGA TGCTACTGTT GGAAGCAGAA GGGGTGTTAG TGAAACAGAA
    TCTATTGATC AAGGGTCTGA ACCTTCCCAG CTGTCATCAG AAACTGAGCA GAGTGATCCT
    GCTCGCACAG AAAGTGTCAG TGAAGCTAGC ACCCGGCAAG AAGGGGAGAG TGATGTGGAA
    GGGGCAGACA GCTCTTGCAA TGAAAGTGCA ACCGTGCGAC GTTCCTCAGT TGAAATGCGC
    TGTTCTTTTG AAAGTGCCAG GTTTCCTGAG ACTCCAGCAT TTTCTTCTCA GGAAGAGGAA
    GAGGGAGCTT GTGCCACTGA TGTGGCCAGG ACTGAACCAG TTGTTGAAAC ACAGGACTCT
    CCAAGTACTT CTGGTTCTTT GCCTGCAGTA CAGTTACCTC AGGAGGAAGT ACAGGAGGCT
    GAGGGAGGTG AATTACAAGA CCAAGAGGAT GCAGAAGAAA TCTGGCAAAG AAGAAGTCTG
    CAGGCAGCAG CTGCCAATAG CCAGTCTCAG GATGAAGAAA CGGAAGGAGC CCAAGCAGCT
    TGTGAGGGTG CCACAGCAGA GGTTGAAGGA GCTACTGGAG GTGCTCAAGC CAATGGCCAT
    CAGCCCTTGA GGTCACTACC TTCAGTACGT CAAGATGTTA GCCGGTACCA GCGAGTGGAT
    GAGGCATTAC CACCAAACTG GGAGGCTCGA ATTGACAGCC ATGGACGGAT ATTTTATGTT
    GACCACGTGA ACAGGACAAC AACTTGGCAG CGACCCACAG CCCCTCCAGC CCCACAGATT
    CTCCAGAGAT CAAATTCCAT ACAGCAAATG GAACAGCTGA ATCGCCGGTA TCAGAGCATC
    CGCCGAACAA TGACTAATGA CAGGCCTGAA GAGCAGACAA GTGCAGTGGA TGGAGCTGGA
    GAGGAAACTG ACTTTCACCA TTCTAATGCA GATTTTCGAA GAGAGAATGT GCTGCCTCAC
    TCTACCTCCA GATCCAGACT TACTTTGTTG CTCCAGTCTC CCCCTGTGAA ATTTCTTATC
    AGCCCAGAGT TCTTTACAGT GCTGCATTCT AACCCTAGTG CCTACCGCAT GTTTACAAAT
    AACACGTGTT TGAAGCACAT GATCACCAAA GTCCGGCGGG ACACCCACCA CTTTGAGCGC
    TACCAGCATA ATCGTGATTT GGTGGGCTTC CTAAACATGT TTGCTAACAA ACAGCTGGAG
    CTGCCAAGAG GTTGGGAAAT GAAACATGAC CATCAGGGCA AGGCTTTCTT TGTTGACCAC
    AATTCCCGCA CGACCACGTT CATTGACCCT CGGCTTCCCT TGCAGAGCAG CAGGCCTACC
    AGTGCTTTAG TCCATCGGCA GCACTTAACT AGGCAACGCA GCCACAGTGC TGGTGAGGTT
    GGAGAGGACT CTCGTCATGC AGGACCACCA GTTCTACCAA GACCGTCTAG TACATTTAAT
    ACAGTCAGCA GAGCTCAGTA CCAGGATGTG GTTCCAGTGG CTTACAATGA CAAGATTGTT
    GCATTTTTGC GGCAGCCCAA CATCTTTGAA ATTCTACAAG AGCGTCAACC AGATCTTACC
    AGGAATCATT CACTCAGGGA GAAGATCCAG TTCATCCGGA CAGAGGGAAC GCCTGGCTTG
    GTGCGTTTAT CCAGTGATGC AGACCTTGTC ATGTTACTCA GCTTGTTCGA AGAGGAGATC
    ATGTCATACG TGCCGCCACA TGCCTTACTT CATCCCAGTT ATTGCCAGTC CCCAAGAGGT
    TCGCCAGTGT CCTCACCCCA GAACTCTCCA GGTACTCAGC GTGCCAATGC CCGAGCTCCA
    GCTCCTTACA AAAGAGATTT TGAAGCCAAG CTGAGAAACT TTTACAGGAA GTTGGAAACT
    AAAGGATATG GACAAGGCCC AGGGAAATTG AAATTAATAA TCAGGAGAGA TCACTTGCTT
    GAAGATGCCT TTAACCAGAT TATGGGTTAT TCAAGAAAAG ACCTGCAGAG AAATAAACTC
    TATGTCACAT TTGTTGGGGA AGAGGGGTTG GACTACAGTG GACCTTCAAG GGAATTTTTT
    TTCCTGGTGT CTAGAGAGCT TTTCAATCCG TATTATGGTT TATTTGAATA TTCAGCCAAC
    GATACCTATA CAGTACAAAT AAGTCCCATG TCTGCTTTTG TAGACAATCA CCATGAATGG
    TTCAGGTTTA GCGGTCGAAT CCTTGGTCTT GCTCTGATAC ACCAATATTT GCTAGATGCC
    TTCTTTACAC GACCCTTTTA TAAAGCCCTC CTCCGAATAC TCTGTGATCT GAGTGACCTG
    GAATATCTTG ATGAGGAGTT TCACCAGAGT TTGCAGTGGA TGAAAGATAA TGATATACAT
    GATATCCTGG ATCTCACATT TACTGTAAAT GAAGAAGTTT TTGGGCAGAT CACAGAACGG
    GAATTAAAGC CAGGGGGTGC CAACATTCCA GTCACAGAAA AAAACAAGAA AGAATATATA
    GAAAGAATGG TAAAATGGAG AATTGAGAGA GGAGTTGTAC AACAGACAGA AAGTCTAGTT
    CGTGGCTTCT ATGAGGTGGT TGATGCAAGA CTTGTGTCTG TATTTGATGC GAGAGAACTG
    GAACTAGTTA TAGCTGGAAC AGCAGAAATT GACTTGAGTG ACTGGAGAAA TAATACAGAA
    TATAGAGGAG GTTATCATGA CAATCACATT GTAATTCGGT GGTTCTGGGC TGCTGTGGAA
    AGATTCAACA ATGAACAACG ACTAAGACTT CTGCAGTTTG TTACAGGCAC ATCCAGCATA
    CCTTATGAGG GGTTTGCCTC TCTAAGAGGG AGCAATGGGC CAAGAAGGTT CTGCGTAGAG
    AAGTGGGGGA AGATCACTGC CCTTCCAAGG GCTCACACTT GTTTCAATCG TCTGGACCTT
    CCCCCTTATC CATCATTCTC TATGCTGTAT GAAAAACTGT TGACAGCAGT AGAAGAGACA
    AGTACTTTTG GACTTGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OGa10877
    Clone ID Related Accession (Same CDS sequence) XM_015289489.1 , XM_015289488.1 , XM_015289489.2 , XM_015289488.2
    Accession Version XM_015289488.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4701bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-16
    Organism Gallus gallus(chicken)
    Product E3 ubiquitin-protein ligase HECW2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006094.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015289488.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGTT CAGCGCGAGA ACATCTGCTG TTTGTGCGAC GTCGCAACCC ACAGCTTCGG 
    TATACTCTAA GCCCAGAGAA TCTGCAGAGT TTGGCATCTC AGGGCACCAT GGCTGAGAAC
    ATGAATTTGC AACGTGCCAA CAGTGACACT GACTTAGTGA CCTCAGACAG CAGGTCTAGC
    TTGACAGCCA GTATGTATGA GTACACCTTG GGGCAATCTC AGAACCTCAT AATTTTCTGG
    GATATTAAGG AAGAAGTTGA TCCAACTGAC TGGATAGGAC TTTATCACAT AGATGAGAAC
    TCTCCAGCCA ATTTCTGGGA TTCCAAGAAT CGGGGAGTGA CTGGCACACA AAAAGGACAA
    ATTGTATGGC GAATTGAACC TGGTCCCTAT TTTATGGAAT CTGAAATAAA GATCTGTTTT
    AAATACTACC ATGGTGTTAG TGGAGCCCTC CGAGCTACTA CTCCATGTAT CACTGTGAAA
    AACCCTGCTG TGATGATGGG AGTAGAAGGC ACAGATGAAG GTGCTTCTGG AGCCCAGCAC
    TCTCGGAAGT TAGTCAGCTT TACACTATCA GATATTAGAG CAGTTGGACT TAAAAAAGGC
    ATGTTCTTCA ACCCTGATCC TTATCTAAAG ATGTCCATCC AGCCGGGCAA GAAAAGTACA
    TTTCCTACAT TTGCTCATCA TGGTCAGGAA AGAAGATCAA CTATCATCAG TAACACAACT
    AACCCTGTTT GGCACAGAGA GAAATATTCT TTTGTTGCAC TTCTAACAGA TGTCCTGGAA
    ATTGAAGTTA AAGACAAATT TGCCAAGAGT CGTCCAATCA TCAAACGCTT TCTTGGGAAG
    TTAACCATAC CAGTTCAGAG ACTGTTGGAG AGACATGCAA TTGGAGACCA GATTCTCAGC
    TATAATCTTG GCAGAAGGCT CCCAGCGGAT CATGTAAGTG GATATCTCCA GTTCAAAGTG
    GAAATCACAT CTTCTGTTCA TGAAGATGCT TCACCAGAAA CAGTTGGCAC TTTGCTAGGA
    GTGAACTCTG TAAATGGAGA CTTGGGCAGC CCCTCTGACG ATGAGGACAT GCCAACAGGA
    CATCATGATA CTTCCACAGT GTGTTCCAAT GGTCCAATCC TTGAGGAATC TTCTGGAGAA
    ACAATCCAGA GGCATCCTTT AAGGACTAGT ACAACACTAG AAACAGATCA GGATGAGTTA
    ACCTCTGGTG CTTCAAGATC GTCACCTACA AGAGGTCGCC AGGACTCACT CAATGACTAT
    TTGGATGCAA TAGAACACAA TAATCATCCC AGACCTGGGA CGTCTGCCTG TGGCGAGCGT
    CCACGTGGAG CCTCCCCAAA ACTGAGGAGT AGCTTCCCTA CTGACACAAG ACTTAATGCA
    ATGCTTCACA TTGACTCAGA TGAGGAGGAG CATGAGCTAC ATCAAGACCT GGGTTTCCCA
    TCTTCCTTGG AAGATGATGG TGGCTTAGTA ATGCTTAATG GTGCTACAAG AAATGTTGAA
    AGAAATGGTT TAAGTTACTC ATCAGATCAG GTAACACAGG GGCCTGCAGA GGGTGAAAAT
    GTTTCAGCCT CTGAAGCTCC TTTGCCCTCA ACTGATGACC AGACAGAGAG TCTCCCAGAG
    CTTCCACCAT CACAGTTAGT TGAAGGTGAA AGCCTGCAAG GTTCTCAGAG TGAAACACCG
    GCAGACCAGG CTGGCAGTGA CTCATATACT GTGCAGGAGG CAGAGCAGCC TCCCAGTAGT
    ACAGATGCAG GAGCTGCCGA TGCTACTGTT GGAAGCAGAA GGGGTGTTAG TGAAACAGAA
    TCTATTGATC AAGGGTCTGA ACCTTCCCAG CTGTCATCAG AAACTGAGCA GAGTGATCCT
    GCTCGCACAG AAAGTGTCAG TGAAGCTAGC ACCCGGCAAG AAGGGGAGAG TGATGTGGAA
    GGGGCAGACA GCTCTTGCAA TGAAAGTGCA ACCGTGCGAC GTTCCTCAGT TGAAATGCGC
    TGTTCTTTTG AAAGTGCCAG GTTTCCTGAG ACTCCAGCAT TTTCTTCTCA GGAAGAGGAA
    GAGGGAGCTT GTGCCACTGA TGTGGCCAGG ACTGAACCAG TTGTTGAAAC ACAGGACTCT
    CCAAGTACTT CTGGTTCTTT GCCTGCAGTA CAGTTACCTC AGGAGGAAGT ACAGGAGGCT
    GAGGGAGGTG AATTACAAGA CCAAGAGGAT GCAGAAGAAA TCTGGCAAAG AAGAAGTCTG
    CAGGCAGCAG CTGCCAATAG CCAGTCTCAG GATGAAGAAA CGGAAGGAGC CCAAGCAGCT
    TGTGAGGGTG CCACAGCAGA GGTTGAAGGA GCTACTGGAG GTGCTCAAGC CAATGGCCAT
    CAGCCCTTGA GGTCACTACC TTCAGTACGT CAAGATGTTA GCCGGTACCA GCGAGTGGAT
    GAGGCATTAC CACCAAACTG GGAGGCTCGA ATTGACAGCC ATGGACGGAT ATTTTATGTT
    GACCACGTGA ACAGGACAAC AACTTGGCAG CGACCCACAG CCCCTCCAGC CCCACAGATT
    CTCCAGAGAT CAAATTCCAT ACAGCAAATG GAACAGCTGA ATCGCCGGTA TCAGAGCATC
    CGCCGAACAA TGACTAATGA CAGGCCTGAA GAGCAGACAA GTGCAGTGGA TGGAGCTGGA
    GAGGAAACTG ACTTTCACCA TTCTAATGCA GATTTTCGAA GAGAGAATGT GCTGCCTCAC
    TCTACCTCCA GATCCAGACT TACTTTGTTG CTCCAGTCTC CCCCTGTGAA ATTTCTTATC
    AGCCCAGAGT TCTTTACAGT GCTGCATTCT AACCCTAGTG CCTACCGCAT GTTTACAAAT
    AACACGTGTT TGAAGCACAT GATCACCAAA GTCCGGCGGG ACACCCACCA CTTTGAGCGC
    TACCAGCATA ATCGTGATTT GGTGGGCTTC CTAAACATGT TTGCTAACAA ACAGCTGGAG
    CTGCCAAGAG GTTGGGAAAT GAAACATGAC CATCAGGGCA AGGCTTTCTT TGTTGACCAC
    AATTCCCGCA CGACCACGTT CATTGACCCT CGGCTTCCCT TGCAGAGCAG CAGGCCTACC
    AGTGCTTTAG TCCATCGGCA GCACTTAACT AGGCAACGCA GCCACAGTGC TGGTGAGGTT
    GGAGAGGACT CTCGTCATGC AGGACCACCA GTTCTACCAA GACCGTCTAG TACATTTAAT
    ACAGTCAGCA GAGCTCAGTA CCAGGATGTG GTTCCAGTGG CTTACAATGA CAAGATTGTT
    GCATTTTTGC GGCAGCCCAA CATCTTTGAA ATTCTACAAG AGCGTCAACC AGATCTTACC
    AGGAATCATT CACTCAGGGA GAAGATCCAG TTCATCCGGA CAGAGGGAAC GCCTGGCTTG
    GTGCGTTTAT CCAGTGATGC AGACCTTGTC ATGTTACTCA GCTTGTTCGA AGAGGAGATC
    ATGTCATACG TGCCGCCACA TGCCTTACTT CATCCCAGTT ATTGCCAGTC CCCAAGAGGT
    TCGCCAGTGT CCTCACCCCA GAACTCTCCA GGTACTCAGC GTGCCAATGC CCGAGCTCCA
    GCTCCTTACA AAAGAGATTT TGAAGCCAAG CTGAGAAACT TTTACAGGAA GTTGGAAACT
    AAAGGATATG GACAAGGCCC AGGGAAATTG AAATTAATAA TCAGGAGAGA TCACTTGCTT
    GAAGATGCCT TTAACCAGAT TATGGGTTAT TCAAGAAAAG ACCTGCAGAG AAATAAACTC
    TATGTCACAT TTGTTGGGGA AGAGGGGTTG GACTACAGTG GACCTTCAAG GGAATTTTTT
    TTCCTGGTGT CTAGAGAGCT TTTCAATCCG TATTATGGTT TATTTGAATA TTCAGCCAAC
    GATACCTATA CAGTACAAAT AAGTCCCATG TCTGCTTTTG TAGACAATCA CCATGAATGG
    TTCAGGTTTA GCGGTCGAAT CCTTGGTCTT GCTCTGATAC ACCAATATTT GCTAGATGCC
    TTCTTTACAC GACCCTTTTA TAAAGCCCTC CTCCGAATAC TCTGTGATCT GAGTGACCTG
    GAATATCTTG ATGAGGAGTT TCACCAGAGT TTGCAGTGGA TGAAAGATAA TGATATACAT
    GATATCCTGG ATCTCACATT TACTGTAAAT GAAGAAGTTT TTGGGCAGAT CACAGAACGG
    GAATTAAAGC CAGGGGGTGC CAACATTCCA GTCACAGAAA AAAACAAGAA AGAATATATA
    GAAAGAATGG TAAAATGGAG AATTGAGAGA GGAGTTGTAC AACAGACAGA AAGTCTAGTT
    CGTGGCTTCT ATGAGGTGGT TGATGCAAGA CTTGTGTCTG TATTTGATGC GAGAGAACTG
    GAACTAGTTA TAGCTGGAAC AGCAGAAATT GACTTGAGTG ACTGGAGAAA TAATACAGAA
    TATAGAGGAG GTTATCATGA CAATCACATT GTAATTCGGT GGTTCTGGGC TGCTGTGGAA
    AGATTCAACA ATGAACAACG ACTAAGACTT CTGCAGTTTG TTACAGGCAC ATCCAGCATA
    CCTTATGAGG GGTTTGCCTC TCTAAGAGGG AGCAATGGGC CAAGAAGGTT CTGCGTAGAG
    AAGTGGGGGA AGATCACTGC CCTTCCAAGG GCTCACACTT GTTTCAATCG TCTGGACCTT
    CCCCCTTATC CATCATTCTC TATGCTGTAT GAAAAACTGT TGACAGCAGT AGAAGAGACA
    AGTACTTTTG GACTTGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_015144974.1
    CDS194..4894
    Translation

    Target ORF information:

    RefSeq Version XM_015289488.2
    Organism Gallus gallus(chicken)
    Definition Gallus gallus HECT, C2 and WW domain containing E3 ubiquitin protein ligase 2 (HECW2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_015289488.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGGCTAGTT CAGCGCGAGA ACATCTGCTG TTTGTGCGAC GTCGCAACCC ACAGCTTCGG 
    TATACTCTAA GCCCAGAGAA TCTGCAGAGT TTGGCATCTC AGGGCACCAT GGCTGAGAAC
    ATGAATTTGC AACGTGCCAA CAGTGACACT GACTTAGTGA CCTCAGACAG CAGGTCTAGC
    TTGACAGCCA GTATGTATGA GTACACCTTG GGGCAATCTC AGAACCTCAT AATTTTCTGG
    GATATTAAGG AAGAAGTTGA TCCAACTGAC TGGATAGGAC TTTATCACAT AGATGAGAAC
    TCTCCAGCCA ATTTCTGGGA TTCCAAGAAT CGGGGAGTGA CTGGCACACA AAAAGGACAA
    ATTGTATGGC GAATTGAACC TGGTCCCTAT TTTATGGAAT CTGAAATAAA GATCTGTTTT
    AAATACTACC ATGGTGTTAG TGGAGCCCTC CGAGCTACTA CTCCATGTAT CACTGTGAAA
    AACCCTGCTG TGATGATGGG AGTAGAAGGC ACAGATGAAG GTGCTTCTGG AGCCCAGCAC
    TCTCGGAAGT TAGTCAGCTT TACACTATCA GATATTAGAG CAGTTGGACT TAAAAAAGGC
    ATGTTCTTCA ACCCTGATCC TTATCTAAAG ATGTCCATCC AGCCGGGCAA GAAAAGTACA
    TTTCCTACAT TTGCTCATCA TGGTCAGGAA AGAAGATCAA CTATCATCAG TAACACAACT
    AACCCTGTTT GGCACAGAGA GAAATATTCT TTTGTTGCAC TTCTAACAGA TGTCCTGGAA
    ATTGAAGTTA AAGACAAATT TGCCAAGAGT CGTCCAATCA TCAAACGCTT TCTTGGGAAG
    TTAACCATAC CAGTTCAGAG ACTGTTGGAG AGACATGCAA TTGGAGACCA GATTCTCAGC
    TATAATCTTG GCAGAAGGCT CCCAGCGGAT CATGTAAGTG GATATCTCCA GTTCAAAGTG
    GAAATCACAT CTTCTGTTCA TGAAGATGCT TCACCAGAAA CAGTTGGCAC TTTGCTAGGA
    GTGAACTCTG TAAATGGAGA CTTGGGCAGC CCCTCTGACG ATGAGGACAT GCCAACAGGA
    CATCATGATA CTTCCACAGT GTGTTCCAAT GGTCCAATCC TTGAGGAATC TTCTGGAGAA
    ACAATCCAGA GGCATCCTTT AAGGACTAGT ACAACACTAG AAACAGATCA GGATGAGTTA
    ACCTCTGGTG CTTCAAGATC GTCACCTACA AGAGGTCGCC AGGACTCACT CAATGACTAT
    TTGGATGCAA TAGAACACAA TAATCATCCC AGACCTGGGA CGTCTGCCTG TGGCGAGCGT
    CCACGTGGAG CCTCCCCAAA ACTGAGGAGT AGCTTCCCTA CTGACACAAG ACTTAATGCA
    ATGCTTCACA TTGACTCAGA TGAGGAGGAG CATGAGCTAC ATCAAGACCT GGGTTTCCCA
    TCTTCCTTGG AAGATGATGG TGGCTTAGTA ATGCTTAATG GTGCTACAAG AAATGTTGAA
    AGAAATGGTT TAAGTTACTC ATCAGATCAG GTAACACAGG GGCCTGCAGA GGGTGAAAAT
    GTTTCAGCCT CTGAAGCTCC TTTGCCCTCA ACTGATGACC AGACAGAGAG TCTCCCAGAG
    CTTCCACCAT CACAGTTAGT TGAAGGTGAA AGCCTGCAAG GTTCTCAGAG TGAAACACCG
    GCAGACCAGG CTGGCAGTGA CTCATATACT GTGCAGGAGG CAGAGCAGCC TCCCAGTAGT
    ACAGATGCAG GAGCTGCCGA TGCTACTGTT GGAAGCAGAA GGGGTGTTAG TGAAACAGAA
    TCTATTGATC AAGGGTCTGA ACCTTCCCAG CTGTCATCAG AAACTGAGCA GAGTGATCCT
    GCTCGCACAG AAAGTGTCAG TGAAGCTAGC ACCCGGCAAG AAGGGGAGAG TGATGTGGAA
    GGGGCAGACA GCTCTTGCAA TGAAAGTGCA ACCGTGCGAC GTTCCTCAGT TGAAATGCGC
    TGTTCTTTTG AAAGTGCCAG GTTTCCTGAG ACTCCAGCAT TTTCTTCTCA GGAAGAGGAA
    GAGGGAGCTT GTGCCACTGA TGTGGCCAGG ACTGAACCAG TTGTTGAAAC ACAGGACTCT
    CCAAGTACTT CTGGTTCTTT GCCTGCAGTA CAGTTACCTC AGGAGGAAGT ACAGGAGGCT
    GAGGGAGGTG AATTACAAGA CCAAGAGGAT GCAGAAGAAA TCTGGCAAAG AAGAAGTCTG
    CAGGCAGCAG CTGCCAATAG CCAGTCTCAG GATGAAGAAA CGGAAGGAGC CCAAGCAGCT
    TGTGAGGGTG CCACAGCAGA GGTTGAAGGA GCTACTGGAG GTGCTCAAGC CAATGGCCAT
    CAGCCCTTGA GGTCACTACC TTCAGTACGT CAAGATGTTA GCCGGTACCA GCGAGTGGAT
    GAGGCATTAC CACCAAACTG GGAGGCTCGA ATTGACAGCC ATGGACGGAT ATTTTATGTT
    GACCACGTGA ACAGGACAAC AACTTGGCAG CGACCCACAG CCCCTCCAGC CCCACAGATT
    CTCCAGAGAT CAAATTCCAT ACAGCAAATG GAACAGCTGA ATCGCCGGTA TCAGAGCATC
    CGCCGAACAA TGACTAATGA CAGGCCTGAA GAGCAGACAA GTGCAGTGGA TGGAGCTGGA
    GAGGAAACTG ACTTTCACCA TTCTAATGCA GATTTTCGAA GAGAGAATGT GCTGCCTCAC
    TCTACCTCCA GATCCAGACT TACTTTGTTG CTCCAGTCTC CCCCTGTGAA ATTTCTTATC
    AGCCCAGAGT TCTTTACAGT GCTGCATTCT AACCCTAGTG CCTACCGCAT GTTTACAAAT
    AACACGTGTT TGAAGCACAT GATCACCAAA GTCCGGCGGG ACACCCACCA CTTTGAGCGC
    TACCAGCATA ATCGTGATTT GGTGGGCTTC CTAAACATGT TTGCTAACAA ACAGCTGGAG
    CTGCCAAGAG GTTGGGAAAT GAAACATGAC CATCAGGGCA AGGCTTTCTT TGTTGACCAC
    AATTCCCGCA CGACCACGTT CATTGACCCT CGGCTTCCCT TGCAGAGCAG CAGGCCTACC
    AGTGCTTTAG TCCATCGGCA GCACTTAACT AGGCAACGCA GCCACAGTGC TGGTGAGGTT
    GGAGAGGACT CTCGTCATGC AGGACCACCA GTTCTACCAA GACCGTCTAG TACATTTAAT
    ACAGTCAGCA GAGCTCAGTA CCAGGATGTG GTTCCAGTGG CTTACAATGA CAAGATTGTT
    GCATTTTTGC GGCAGCCCAA CATCTTTGAA ATTCTACAAG AGCGTCAACC AGATCTTACC
    AGGAATCATT CACTCAGGGA GAAGATCCAG TTCATCCGGA CAGAGGGAAC GCCTGGCTTG
    GTGCGTTTAT CCAGTGATGC AGACCTTGTC ATGTTACTCA GCTTGTTCGA AGAGGAGATC
    ATGTCATACG TGCCGCCACA TGCCTTACTT CATCCCAGTT ATTGCCAGTC CCCAAGAGGT
    TCGCCAGTGT CCTCACCCCA GAACTCTCCA GGTACTCAGC GTGCCAATGC CCGAGCTCCA
    GCTCCTTACA AAAGAGATTT TGAAGCCAAG CTGAGAAACT TTTACAGGAA GTTGGAAACT
    AAAGGATATG GACAAGGCCC AGGGAAATTG AAATTAATAA TCAGGAGAGA TCACTTGCTT
    GAAGATGCCT TTAACCAGAT TATGGGTTAT TCAAGAAAAG ACCTGCAGAG AAATAAACTC
    TATGTCACAT TTGTTGGGGA AGAGGGGTTG GACTACAGTG GACCTTCAAG GGAATTTTTT
    TTCCTGGTGT CTAGAGAGCT TTTCAATCCG TATTATGGTT TATTTGAATA TTCAGCCAAC
    GATACCTATA CAGTACAAAT AAGTCCCATG TCTGCTTTTG TAGACAATCA CCATGAATGG
    TTCAGGTTTA GCGGTCGAAT CCTTGGTCTT GCTCTGATAC ACCAATATTT GCTAGATGCC
    TTCTTTACAC GACCCTTTTA TAAAGCCCTC CTCCGAATAC TCTGTGATCT GAGTGACCTG
    GAATATCTTG ATGAGGAGTT TCACCAGAGT TTGCAGTGGA TGAAAGATAA TGATATACAT
    GATATCCTGG ATCTCACATT TACTGTAAAT GAAGAAGTTT TTGGGCAGAT CACAGAACGG
    GAATTAAAGC CAGGGGGTGC CAACATTCCA GTCACAGAAA AAAACAAGAA AGAATATATA
    GAAAGAATGG TAAAATGGAG AATTGAGAGA GGAGTTGTAC AACAGACAGA AAGTCTAGTT
    CGTGGCTTCT ATGAGGTGGT TGATGCAAGA CTTGTGTCTG TATTTGATGC GAGAGAACTG
    GAACTAGTTA TAGCTGGAAC AGCAGAAATT GACTTGAGTG ACTGGAGAAA TAATACAGAA
    TATAGAGGAG GTTATCATGA CAATCACATT GTAATTCGGT GGTTCTGGGC TGCTGTGGAA
    AGATTCAACA ATGAACAACG ACTAAGACTT CTGCAGTTTG TTACAGGCAC ATCCAGCATA
    CCTTATGAGG GGTTTGCCTC TCTAAGAGGG AGCAATGGGC CAAGAAGGTT CTGCGTAGAG
    AAGTGGGGGA AGATCACTGC CCTTCCAAGG GCTCACACTT GTTTCAATCG TCTGGACCTT
    CCCCCTTATC CATCATTCTC TATGCTGTAT GAAAAACTGT TGACAGCAGT AGAAGAGACA
    AGTACTTTTG GACTTGAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.