SCN1A cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCN1A
Entrez Gene ID	529590
Full Name	sodium voltage-gated channel alpha subunit 1
General protein information	Preferred Names sodium voltage-gated channel alpha subunit 1 Names sodium channel protein type 1 subunit alpha sodium channel, voltage-gated, type I, alpha subunit
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_010801984.2	XP_010800286.1	sodium channel protein type 1 subunit alpha isoform X1
NM_001193218.2	NP_001180147.1	sodium channel protein type 1 subunit alpha
XM_024975472.1	XP_024831240.1	sodium channel protein type 1 subunit alpha isoform X2
XM_015475298.1	XP_015330784.1	sodium channel protein type 1 subunit alpha isoform X2
XM_024975464.1	XP_024831232.1	sodium channel protein type 1 subunit alpha isoform X1
XM_005202445.3	XP_005202502.1	sodium channel protein type 1 subunit alpha isoform X1
XM_015475302.1	XP_015330788.1	sodium channel protein type 1 subunit alpha isoform X3
NM_001193218.1	NP_001180147.1	sodium channel protein type 1 subunit alpha

Pathways from BioSystems

Homologies

Homo sapiens (human)	SCN1A	NP_001159435.1
Mus musculus (house mouse)	Scn1a	NP_061203.2
Gallus gallus (chicken)	SCN1A	XP_003641634.1
	SCN1A	XP_001101023.1
Bos taurus (cattle)	SCN1A	NP_001180147.1
Pan troglodytes (chimpanzee)	SCN1A	XP_003309330.1
Canis lupus familiaris (dog)	SCN1A	XP_005640320.1
Rattus norvegicus (Norway rat)	Scn1a	NP_110502.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SCN1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCN1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa227375	NM_001193218.2 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 1 (SCN1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa227375	XM_024975464.1 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa227376	XM_024975472.1 Latest version!	Bos taurus sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OBa36949	NM_001193218.1 Latest version!	Bos taurus sodium channel, voltage-gated, type I, alpha subunit (SCN1A), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa16416	XM_015475298.1 Latest version!	Bos taurus sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa16417	XM_015475302.1 Latest version!	Bos taurus sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
OBa16415	XM_010801984.2 Latest version!	Bos taurus sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00
OBa16415	XM_005202445.3 Latest version!	Bos taurus sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCN1A ( NM_001193218.2 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180147.1 Bos taurus sodium voltage-gated channel alpha subunit 1 (SCN1A), mRNA.

Price & Availability↑

CloneID	OBa227375
Clone ID Related Accession (Same CDS sequence)	NM_001193218.2 , XM_024975464.1
Accession Version	NM_001193218.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-28
Organism	Bos taurus(cattle)
Product	sodium channel protein type 1 subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000002.1. On May 29, 2018 this sequence version replaced NM_001193218.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145437, SAMN03145443 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTGTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCGGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTTCA TGTTTCCATG GACTTTCTAG AAGATCCATC TCAAAGGCAA 
AGAGCAATGA GCATAGCCAG CATTTTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCG 
CCATACTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC TTTTGTTGAC 
CTGGCCATCA CCATCTGCAT TGTCTTAAAC ACTCTCTTCA TGGCCATGGA GCACTATCCC 
ATGACAGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGATGGTTT TATTGTAACG CTTAGCCTGG TAGAACTTGG CCTGGCCAAC 
GTGGAAGGAT TATCAGTTCT CCGTTCATTC CGTTTGCTTC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CATTAAATAT GCTAATAAAG ATCATCGGCA ATTCAGTGGG GGCTCTGGGA 
AACCTAACCT TGGTGTTAGC CATCATCGTC TTCATTTTTG CTGTGGTTGG CATGCAGCTC 
TTTGGTAAAA GTTACAAAGA TTGTGTCTGC AAGATCAACA GTGAATGTAA GCTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGGGTGCT GTGTGGAGAG 
TGGATAGAGA CCATGTGGGA CTGCATGGAG GTCGCCGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTCAT TGGAAACCTG GTGGTCCTGA ACCTCTTTTT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCG GCCACTGATG ATGATAATGA AATGAACAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAATAGCTT ATGTGAAGAG AAAAATATAT 
GAATTTATTC AGCAGTCCTT TGTTAGAAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAGGAA AGACAATTGT ATGTCCAATC ATACAACAGA GATTGGGAAA 
GACCTTGACT ATCTTAAAGA CGTAAATGGA ACCACAAGTG GAATAGGAAC CGGCAGCAGT 
GTTGAAAAGT ACATTATTGA TGAAAGTGAT TACATGTCCT TCATAAACAA TCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACAGAGGAC 
TTCAGTAGTG AATCAGATCT GGAAGAAAGC AAAGAGAAGC TTAATGAAAG CAGTAGTTCA 
TCTGAAGGTA GCACTGTAGA CATTGGAGCA CCTGCCGAGG AACAGCCTGT GGTGGAACCC 
GAGGAAACCC TTGAACCTGA AGCCTGTTTC ACTGAAGGCT GTGTGCAAAG ATTCAAATGT 
TGTCAAATCA GTGTGGAAGA AGGGAGAGGA AAACAGTGGT GGAATCTGAG AAGAACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCAC TGGCATTTGA GGATATATAT ATTGACCAGC GAAAGACAAT CAAAACAATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTAGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGTGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC ACGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTACTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTGTTAA CACCACAACT GGTGACATGT TTGACATCAG CGAAGTGAAT 
AATCATTCAG ATTGCCTAAT ACTAATAGAA AGAAATGAGA CTGCCCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTT 
AAGGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAGG AAAGTCTATA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAAAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCAAAAAA GCCACAAAAG CCTATACCTC GACCAGGAAA CAAATTCCAA 
GGAATGGTCT TTGACTTCGT CACCAGACAA GTTTTTGACA TCAGCATCAT GATTCTTATC 
TGTCTCAACA TGGTCACCAT GATGGTGGAA ACCGACGACC AGAGCGACTG TGTGACTAGC 
ATTTTGTCCC GCATCAACTT GGTGTTCATT GTGCTGTTCA CTGGGGAGTG TGTGCTGAAG 
CTCATCTCCC TCCGCCATTA CTATTTTACT ATAGGCTGGA ATATCTTTGA TTTTGTGGTG 
GTCATTCTCT CCATAGTAGG TATGTTTCTG GCTGAGCTGA TAGAGAAATA TTTTGTGTCC 
CCTACCTTGT TCCGAGTGAT CCGTCTTGCC AGGATTGGCC GAATCCTGCG TCTGATCAAA 
GGGGCTAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTGCTCTT CCTGGTCATG TTCATCTATG CCATCTTTGG AATGTCCAAC 
TTCGCCTATG TTAAGAGGGA GGTTGGAATT GATGACATGT TTAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTGTT CCAGATTACC ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCTATTCTCA ACAGTAAACC ACCCGATTGT GACCCTAATA AAGTTAACCC TGGCAGCTCA 
GTTAAAGGAG ACTGTGGGAA CCCATCTGTT GGGATTTTCT TCTTTGTCAG TTACATCATC 
ATATCATTTC TAGTCGTGGT GAACATGTAT ATAGCTGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGCGCAGA GCCCCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA CGCCACCCAG TTCATGGAAT TTGAAAAGCT ATCTCAGTTT 
GCAGCTGCTC TTGAACCACC GCTCAATTTG CCACAACCGA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTAAG TGGTGATCGA ATCCACTGTC TTGACATTTT ATTTGCTTTT 
ACGAAGCGGG TTCTGGGGGA GAGTGGAGAG ATGGATGCCC TTCGAATACA GATGGAAGAG 
AGATTCATGG CCTCCAACCC ATCGAAAGTC TCCTATCAGC CAATAACTAC GACTTTAAAG 
AGAAAACAAG AGGAAGTGTC TGCTGTTATT ATTCAGCGTG CATACAGGCG CCACCTTTTA 
AAGCGAACTG TAAAACAGGC ATCATTTACG TACAACAAAA ACAAAATCAA GGGTGGGGCT 
AATCTTCTTG TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC AATTACAGAA 
AAAACTGATC TGACCATGTC CACAGCAGCT TGTCCACCCT CCTATGATCG GGTAACAAAA 
CCAATTGTGG AAAAGCACGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180147.1
CDS	1..5997
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDEHGPKPNSDLEAGKNLPFIYGDVPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHTVEKNITKNYNGTFVNETRNEFDWKSYIQDSRYHYFLEGFSDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAVTASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKINSECKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGIAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCMSNHTTEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVVEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDMFDISEVNNHSDCLILIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDCVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001193218.2
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 1 (SCN1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193218.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTGTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCGGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTTCA TGTTTCCATG GACTTTCTAG AAGATCCATC TCAAAGGCAA 
AGAGCAATGA GCATAGCCAG CATTTTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCG 
CCATACTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC TTTTGTTGAC 
CTGGCCATCA CCATCTGCAT TGTCTTAAAC ACTCTCTTCA TGGCCATGGA GCACTATCCC 
ATGACAGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGATGGTTT TATTGTAACG CTTAGCCTGG TAGAACTTGG CCTGGCCAAC 
GTGGAAGGAT TATCAGTTCT CCGTTCATTC CGTTTGCTTC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CATTAAATAT GCTAATAAAG ATCATCGGCA ATTCAGTGGG GGCTCTGGGA 
AACCTAACCT TGGTGTTAGC CATCATCGTC TTCATTTTTG CTGTGGTTGG CATGCAGCTC 
TTTGGTAAAA GTTACAAAGA TTGTGTCTGC AAGATCAACA GTGAATGTAA GCTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGGGTGCT GTGTGGAGAG 
TGGATAGAGA CCATGTGGGA CTGCATGGAG GTCGCCGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTCAT TGGAAACCTG GTGGTCCTGA ACCTCTTTTT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCG GCCACTGATG ATGATAATGA AATGAACAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAATAGCTT ATGTGAAGAG AAAAATATAT 
GAATTTATTC AGCAGTCCTT TGTTAGAAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAGGAA AGACAATTGT ATGTCCAATC ATACAACAGA GATTGGGAAA 
GACCTTGACT ATCTTAAAGA CGTAAATGGA ACCACAAGTG GAATAGGAAC CGGCAGCAGT 
GTTGAAAAGT ACATTATTGA TGAAAGTGAT TACATGTCCT TCATAAACAA TCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACAGAGGAC 
TTCAGTAGTG AATCAGATCT GGAAGAAAGC AAAGAGAAGC TTAATGAAAG CAGTAGTTCA 
TCTGAAGGTA GCACTGTAGA CATTGGAGCA CCTGCCGAGG AACAGCCTGT GGTGGAACCC 
GAGGAAACCC TTGAACCTGA AGCCTGTTTC ACTGAAGGCT GTGTGCAAAG ATTCAAATGT 
TGTCAAATCA GTGTGGAAGA AGGGAGAGGA AAACAGTGGT GGAATCTGAG AAGAACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCAC TGGCATTTGA GGATATATAT ATTGACCAGC GAAAGACAAT CAAAACAATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTAGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGTGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC ACGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTACTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTGTTAA CACCACAACT GGTGACATGT TTGACATCAG CGAAGTGAAT 
AATCATTCAG ATTGCCTAAT ACTAATAGAA AGAAATGAGA CTGCCCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTT 
AAGGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAGG AAAGTCTATA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAAAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCAAAAAA GCCACAAAAG CCTATACCTC GACCAGGAAA CAAATTCCAA 
GGAATGGTCT TTGACTTCGT CACCAGACAA GTTTTTGACA TCAGCATCAT GATTCTTATC 
TGTCTCAACA TGGTCACCAT GATGGTGGAA ACCGACGACC AGAGCGACTG TGTGACTAGC 
ATTTTGTCCC GCATCAACTT GGTGTTCATT GTGCTGTTCA CTGGGGAGTG TGTGCTGAAG 
CTCATCTCCC TCCGCCATTA CTATTTTACT ATAGGCTGGA ATATCTTTGA TTTTGTGGTG 
GTCATTCTCT CCATAGTAGG TATGTTTCTG GCTGAGCTGA TAGAGAAATA TTTTGTGTCC 
CCTACCTTGT TCCGAGTGAT CCGTCTTGCC AGGATTGGCC GAATCCTGCG TCTGATCAAA 
GGGGCTAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTGCTCTT CCTGGTCATG TTCATCTATG CCATCTTTGG AATGTCCAAC 
TTCGCCTATG TTAAGAGGGA GGTTGGAATT GATGACATGT TTAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTGTT CCAGATTACC ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCTATTCTCA ACAGTAAACC ACCCGATTGT GACCCTAATA AAGTTAACCC TGGCAGCTCA 
GTTAAAGGAG ACTGTGGGAA CCCATCTGTT GGGATTTTCT TCTTTGTCAG TTACATCATC 
ATATCATTTC TAGTCGTGGT GAACATGTAT ATAGCTGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGCGCAGA GCCCCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA CGCCACCCAG TTCATGGAAT TTGAAAAGCT ATCTCAGTTT 
GCAGCTGCTC TTGAACCACC GCTCAATTTG CCACAACCGA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTAAG TGGTGATCGA ATCCACTGTC TTGACATTTT ATTTGCTTTT 
ACGAAGCGGG TTCTGGGGGA GAGTGGAGAG ATGGATGCCC TTCGAATACA GATGGAAGAG 
AGATTCATGG CCTCCAACCC ATCGAAAGTC TCCTATCAGC CAATAACTAC GACTTTAAAG 
AGAAAACAAG AGGAAGTGTC TGCTGTTATT ATTCAGCGTG CATACAGGCG CCACCTTTTA 
AAGCGAACTG TAAAACAGGC ATCATTTACG TACAACAAAA ACAAAATCAA GGGTGGGGCT 
AATCTTCTTG TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC AATTACAGAA 
AAAACTGATC TGACCATGTC CACAGCAGCT TGTCCACCCT CCTATGATCG GGTAACAAAA 
CCAATTGTGG AAAAGCACGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_024975464.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831232.1 Bos taurus sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X1, mRNA.

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CloneID	OBa227375
Clone ID Related Accession (Same CDS sequence)	NM_001193218.2 , XM_024975464.1
Accession Version	XM_024975464.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium channel protein type 1 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTGTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCGGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTTCA TGTTTCCATG GACTTTCTAG AAGATCCATC TCAAAGGCAA 
AGAGCAATGA GCATAGCCAG CATTTTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCG 
CCATACTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC TTTTGTTGAC 
CTGGCCATCA CCATCTGCAT TGTCTTAAAC ACTCTCTTCA TGGCCATGGA GCACTATCCC 
ATGACAGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGATGGTTT TATTGTAACG CTTAGCCTGG TAGAACTTGG CCTGGCCAAC 
GTGGAAGGAT TATCAGTTCT CCGTTCATTC CGTTTGCTTC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CATTAAATAT GCTAATAAAG ATCATCGGCA ATTCAGTGGG GGCTCTGGGA 
AACCTAACCT TGGTGTTAGC CATCATCGTC TTCATTTTTG CTGTGGTTGG CATGCAGCTC 
TTTGGTAAAA GTTACAAAGA TTGTGTCTGC AAGATCAACA GTGAATGTAA GCTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGGGTGCT GTGTGGAGAG 
TGGATAGAGA CCATGTGGGA CTGCATGGAG GTCGCCGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTCAT TGGAAACCTG GTGGTCCTGA ACCTCTTTTT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCG GCCACTGATG ATGATAATGA AATGAACAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAATAGCTT ATGTGAAGAG AAAAATATAT 
GAATTTATTC AGCAGTCCTT TGTTAGAAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAGGAA AGACAATTGT ATGTCCAATC ATACAACAGA GATTGGGAAA 
GACCTTGACT ATCTTAAAGA CGTAAATGGA ACCACAAGTG GAATAGGAAC CGGCAGCAGT 
GTTGAAAAGT ACATTATTGA TGAAAGTGAT TACATGTCCT TCATAAACAA TCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACAGAGGAC 
TTCAGTAGTG AATCAGATCT GGAAGAAAGC AAAGAGAAGC TTAATGAAAG CAGTAGTTCA 
TCTGAAGGTA GCACTGTAGA CATTGGAGCA CCTGCCGAGG AACAGCCTGT GGTGGAACCC 
GAGGAAACCC TTGAACCTGA AGCCTGTTTC ACTGAAGGCT GTGTGCAAAG ATTCAAATGT 
TGTCAAATCA GTGTGGAAGA AGGGAGAGGA AAACAGTGGT GGAATCTGAG AAGAACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCAC TGGCATTTGA GGATATATAT ATTGACCAGC GAAAGACAAT CAAAACAATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTAGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGTGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC ACGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTACTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTGTTAA CACCACAACT GGTGACATGT TTGACATCAG CGAAGTGAAT 
AATCATTCAG ATTGCCTAAT ACTAATAGAA AGAAATGAGA CTGCCCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTT 
AAGGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAGG AAAGTCTATA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAAAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCAAAAAA GCCACAAAAG CCTATACCTC GACCAGGAAA CAAATTCCAA 
GGAATGGTCT TTGACTTCGT CACCAGACAA GTTTTTGACA TCAGCATCAT GATTCTTATC 
TGTCTCAACA TGGTCACCAT GATGGTGGAA ACCGACGACC AGAGCGACTG TGTGACTAGC 
ATTTTGTCCC GCATCAACTT GGTGTTCATT GTGCTGTTCA CTGGGGAGTG TGTGCTGAAG 
CTCATCTCCC TCCGCCATTA CTATTTTACT ATAGGCTGGA ATATCTTTGA TTTTGTGGTG 
GTCATTCTCT CCATAGTAGG TATGTTTCTG GCTGAGCTGA TAGAGAAATA TTTTGTGTCC 
CCTACCTTGT TCCGAGTGAT CCGTCTTGCC AGGATTGGCC GAATCCTGCG TCTGATCAAA 
GGGGCTAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTGCTCTT CCTGGTCATG TTCATCTATG CCATCTTTGG AATGTCCAAC 
TTCGCCTATG TTAAGAGGGA GGTTGGAATT GATGACATGT TTAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTGTT CCAGATTACC ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCTATTCTCA ACAGTAAACC ACCCGATTGT GACCCTAATA AAGTTAACCC TGGCAGCTCA 
GTTAAAGGAG ACTGTGGGAA CCCATCTGTT GGGATTTTCT TCTTTGTCAG TTACATCATC 
ATATCATTTC TAGTCGTGGT GAACATGTAT ATAGCTGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGCGCAGA GCCCCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA CGCCACCCAG TTCATGGAAT TTGAAAAGCT ATCTCAGTTT 
GCAGCTGCTC TTGAACCACC GCTCAATTTG CCACAACCGA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTAAG TGGTGATCGA ATCCACTGTC TTGACATTTT ATTTGCTTTT 
ACGAAGCGGG TTCTGGGGGA GAGTGGAGAG ATGGATGCCC TTCGAATACA GATGGAAGAG 
AGATTCATGG CCTCCAACCC ATCGAAAGTC TCCTATCAGC CAATAACTAC GACTTTAAAG 
AGAAAACAAG AGGAAGTGTC TGCTGTTATT ATTCAGCGTG CATACAGGCG CCACCTTTTA 
AAGCGAACTG TAAAACAGGC ATCATTTACG TACAACAAAA ACAAAATCAA GGGTGGGGCT 
AATCTTCTTG TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC AATTACAGAA 
AAAACTGATC TGACCATGTC CACAGCAGCT TGTCCACCCT CCTATGATCG GGTAACAAAA 
CCAATTGTGG AAAAGCACGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831232.1
CDS	315..6311
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDEHGPKPNSDLEAGKNLPFIYGDVPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHTVEKNITKNYNGTFVNETRNEFDWKSYIQDSRYHYFLEGFSDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAVTASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKINSECKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGIAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCMSNHTTEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVVEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDMFDISEVNNHSDCLILIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDCVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_024975464.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975464.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTGTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCGGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTTCA TGTTTCCATG GACTTTCTAG AAGATCCATC TCAAAGGCAA 
AGAGCAATGA GCATAGCCAG CATTTTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCG 
CCATACTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC TTTTGTTGAC 
CTGGCCATCA CCATCTGCAT TGTCTTAAAC ACTCTCTTCA TGGCCATGGA GCACTATCCC 
ATGACAGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGATGGTTT TATTGTAACG CTTAGCCTGG TAGAACTTGG CCTGGCCAAC 
GTGGAAGGAT TATCAGTTCT CCGTTCATTC CGTTTGCTTC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CATTAAATAT GCTAATAAAG ATCATCGGCA ATTCAGTGGG GGCTCTGGGA 
AACCTAACCT TGGTGTTAGC CATCATCGTC TTCATTTTTG CTGTGGTTGG CATGCAGCTC 
TTTGGTAAAA GTTACAAAGA TTGTGTCTGC AAGATCAACA GTGAATGTAA GCTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGGGTGCT GTGTGGAGAG 
TGGATAGAGA CCATGTGGGA CTGCATGGAG GTCGCCGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTCAT TGGAAACCTG GTGGTCCTGA ACCTCTTTTT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCG GCCACTGATG ATGATAATGA AATGAACAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAATAGCTT ATGTGAAGAG AAAAATATAT 
GAATTTATTC AGCAGTCCTT TGTTAGAAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAGGAA AGACAATTGT ATGTCCAATC ATACAACAGA GATTGGGAAA 
GACCTTGACT ATCTTAAAGA CGTAAATGGA ACCACAAGTG GAATAGGAAC CGGCAGCAGT 
GTTGAAAAGT ACATTATTGA TGAAAGTGAT TACATGTCCT TCATAAACAA TCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACAGAGGAC 
TTCAGTAGTG AATCAGATCT GGAAGAAAGC AAAGAGAAGC TTAATGAAAG CAGTAGTTCA 
TCTGAAGGTA GCACTGTAGA CATTGGAGCA CCTGCCGAGG AACAGCCTGT GGTGGAACCC 
GAGGAAACCC TTGAACCTGA AGCCTGTTTC ACTGAAGGCT GTGTGCAAAG ATTCAAATGT 
TGTCAAATCA GTGTGGAAGA AGGGAGAGGA AAACAGTGGT GGAATCTGAG AAGAACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCAC TGGCATTTGA GGATATATAT ATTGACCAGC GAAAGACAAT CAAAACAATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTAGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGTGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC ACGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTACTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTGTTAA CACCACAACT GGTGACATGT TTGACATCAG CGAAGTGAAT 
AATCATTCAG ATTGCCTAAT ACTAATAGAA AGAAATGAGA CTGCCCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTT 
AAGGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAGG AAAGTCTATA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAAAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCAAAAAA GCCACAAAAG CCTATACCTC GACCAGGAAA CAAATTCCAA 
GGAATGGTCT TTGACTTCGT CACCAGACAA GTTTTTGACA TCAGCATCAT GATTCTTATC 
TGTCTCAACA TGGTCACCAT GATGGTGGAA ACCGACGACC AGAGCGACTG TGTGACTAGC 
ATTTTGTCCC GCATCAACTT GGTGTTCATT GTGCTGTTCA CTGGGGAGTG TGTGCTGAAG 
CTCATCTCCC TCCGCCATTA CTATTTTACT ATAGGCTGGA ATATCTTTGA TTTTGTGGTG 
GTCATTCTCT CCATAGTAGG TATGTTTCTG GCTGAGCTGA TAGAGAAATA TTTTGTGTCC 
CCTACCTTGT TCCGAGTGAT CCGTCTTGCC AGGATTGGCC GAATCCTGCG TCTGATCAAA 
GGGGCTAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTGCTCTT CCTGGTCATG TTCATCTATG CCATCTTTGG AATGTCCAAC 
TTCGCCTATG TTAAGAGGGA GGTTGGAATT GATGACATGT TTAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTGTT CCAGATTACC ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCTATTCTCA ACAGTAAACC ACCCGATTGT GACCCTAATA AAGTTAACCC TGGCAGCTCA 
GTTAAAGGAG ACTGTGGGAA CCCATCTGTT GGGATTTTCT TCTTTGTCAG TTACATCATC 
ATATCATTTC TAGTCGTGGT GAACATGTAT ATAGCTGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGCGCAGA GCCCCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA CGCCACCCAG TTCATGGAAT TTGAAAAGCT ATCTCAGTTT 
GCAGCTGCTC TTGAACCACC GCTCAATTTG CCACAACCGA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTAAG TGGTGATCGA ATCCACTGTC TTGACATTTT ATTTGCTTTT 
ACGAAGCGGG TTCTGGGGGA GAGTGGAGAG ATGGATGCCC TTCGAATACA GATGGAAGAG 
AGATTCATGG CCTCCAACCC ATCGAAAGTC TCCTATCAGC CAATAACTAC GACTTTAAAG 
AGAAAACAAG AGGAAGTGTC TGCTGTTATT ATTCAGCGTG CATACAGGCG CCACCTTTTA 
AAGCGAACTG TAAAACAGGC ATCATTTACG TACAACAAAA ACAAAATCAA GGGTGGGGCT 
AATCTTCTTG TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC AATTACAGAA 
AAAACTGATC TGACCATGTC CACAGCAGCT TGTCCACCCT CCTATGATCG GGTAACAAAA 
CCAATTGTGG AAAAGCACGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_024975472.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024831240.1 Bos taurus sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X2, mRNA.

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CloneID	OBa227376
Clone ID Related Accession (Same CDS sequence)	XM_024975472.1
Accession Version	XM_024975472.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	sodium channel protein type 1 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037329.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTGTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCGGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTTCA TGTTTCCATG GACTTTCTAG AAGATCCATC TCAAAGGCAA 
AGAGCAATGA GCATAGCCAG CATTTTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCG 
CCATACTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC TTTTGTTGAC 
CTGGCCATCA CCATCTGCAT TGTCTTAAAC ACTCTCTTCA TGGCCATGGA GCACTATCCC 
ATGACAGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGATGGTTT TATTGTAACG CTTAGCCTGG TAGAACTTGG CCTGGCCAAC 
GTGGAAGGAT TATCAGTTCT CCGTTCATTC CGTTTGCTTC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CATTAAATAT GCTAATAAAG ATCATCGGCA ATTCAGTGGG GGCTCTGGGA 
AACCTAACCT TGGTGTTAGC CATCATCGTC TTCATTTTTG CTGTGGTTGG CATGCAGCTC 
TTTGGTAAAA GTTACAAAGA TTGTGTCTGC AAGATCAACA GTGAATGTAA GCTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGGGTGCT GTGTGGAGAG 
TGGATAGAGA CCATGTGGGA CTGCATGGAG GTCGCCGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTCAT TGGAAACCTG GTGGTCCTGA ACCTCTTTTT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCG GCCACTGATG ATGATAATGA AATGAACAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAATAGCTT ATGTGAAGAG AAAAATATAT 
GAATTTATTC AGCAGTCCTT TGTTAGAAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAGGAA AGACAATTGT ATGTCCAATC ATACAACAGA GATTGGGAAA 
GACCTTGACT ATCTTAAAGA CGTAAATGGA ACCACAAGTG GAATAGGAAC CGGCAGCAGT 
GTTGAAAAGT ACATTATTGA TGAAAGTGAT TACATGTCCT TCATAAACAA TCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACAGAGGAC 
TTCAGTAGTG AATCAGATCT GGAAGAAAGC AAAGAGAAGC TTAATGAAAG CAGTAGTTCA 
TCTGAAGGTA GCACTGTAGA CATTGGAGCA CCTGCCGAGG AACAGCCTGT GGTGGAACCC 
GAGGAAACCC TTGAACCTGA AGCCTGTTTC ACTGAAGGCT GTGTGCAAAG ATTCAAATGT 
TGTCAAATCA GTGTGGAAGA AGGGAGAGGA AAACAGTGGT GGAATCTGAG AAGAACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCAC TGGCATTTGA GGATATATAT ATTGACCAGC GAAAGACAAT CAAAACAATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTAGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGTGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC ACGATTTGAA 
GGGATGAGGG CCACATTTAA GGGATGGATG GATATAATGT ATGCAGCAGT TGATTCCAGA 
AATGTGGAAC TCCAGCCTAA GTATGAGGAA AGTCTATACA TGTATCTTTA CTTTGTTATT 
TTCATCATCT TTGGGTCCTT CTTCACCTTG AACCTGTTTA TTGGTGTCAT CATAGATAAT 
TTCAACCAGC AAAAAAAGAA GTTTGGAGGT CAAGACATCT TTATGACAGA AGAACAGAAG 
AAATACTATA ATGCAATGAA AAAATTAGGA TCAAAAAAGC CACAAAAGCC TATACCTCGA 
CCAGGAAACA AATTCCAAGG AATGGTCTTT GACTTCGTCA CCAGACAAGT TTTTGACATC 
AGCATCATGA TTCTTATCTG TCTCAACATG GTCACCATGA TGGTGGAAAC CGACGACCAG 
AGCGACTGTG TGACTAGCAT TTTGTCCCGC ATCAACTTGG TGTTCATTGT GCTGTTCACT 
GGGGAGTGTG TGCTGAAGCT CATCTCCCTC CGCCATTACT ATTTTACTAT AGGCTGGAAT 
ATCTTTGATT TTGTGGTGGT CATTCTCTCC ATAGTAGGTA TGTTTCTGGC TGAGCTGATA 
GAGAAATATT TTGTGTCCCC TACCTTGTTC CGAGTGATCC GTCTTGCCAG GATTGGCCGA 
ATCCTGCGTC TGATCAAAGG GGCTAAGGGG ATCCGCACGC TGCTCTTTGC TTTGATGATG 
TCCCTTCCTG CGTTGTTTAA CATCGGCCTC CTGCTCTTCC TGGTCATGTT CATCTATGCC 
ATCTTTGGAA TGTCCAACTT CGCCTATGTT AAGAGGGAGG TTGGAATTGA TGACATGTTT 
AACTTTGAGA CCTTTGGCAA CAGCATGATC TGCCTGTTCC AGATTACCAC CTCTGCTGGC 
TGGGATGGAT TGCTAGCACC TATTCTCAAC AGTAAACCAC CCGATTGTGA CCCTAATAAA 
GTTAACCCTG GCAGCTCAGT TAAAGGAGAC TGTGGGAACC CATCTGTTGG GATTTTCTTC 
TTTGTCAGTT ACATCATCAT ATCATTTCTA GTCGTGGTGA ACATGTATAT AGCTGTCATC 
CTGGAGAACT TCAGTGTTGC TACTGAAGAA AGCGCAGAGC CCCTGAGTGA GGATGACTTT 
GAGATGTTCT ATGAGGTTTG GGAGAAGTTT GATCCCGACG CCACCCAGTT CATGGAATTT 
GAAAAGCTAT CTCAGTTTGC AGCTGCTCTT GAACCACCGC TCAATTTGCC ACAACCGAAC 
AAACTCCAGC TCATTGCCAT GGATTTGCCC ATGGTAAGTG GTGATCGAAT CCACTGTCTT 
GACATTTTAT TTGCTTTTAC GAAGCGGGTT CTGGGGGAGA GTGGAGAGAT GGATGCCCTT 
CGAATACAGA TGGAAGAGAG ATTCATGGCC TCCAACCCAT CGAAAGTCTC CTATCAGCCA 
ATAACTACGA CTTTAAAGAG AAAACAAGAG GAAGTGTCTG CTGTTATTAT TCAGCGTGCA 
TACAGGCGCC ACCTTTTAAA GCGAACTGTA AAACAGGCAT CATTTACGTA CAACAAAAAC 
AAAATCAAGG GTGGGGCTAA TCTTCTTGTA AAAGAAGACA TGATAATTGA CAGAATAAAT 
GAAAACTCAA TTACAGAAAA AACTGATCTG ACCATGTCCA CAGCAGCTTG TCCACCCTCC 
TATGATCGGG TAACAAAACC AATTGTGGAA AAGCACGAGC AAGAAGGCAA AGATGAAAAA 
GCCAAAGGGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024831240.1
CDS	315..6029
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDEHGPKPNSDLEAGKNLPFIYGDVPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHTVEKNITKNYNGTFVNETRNEFDWKSYIQDSRYHYFLEGFSDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAVTASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKINSECKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGIAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCMSNHTTEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVVEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDCVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_024975472.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024975472.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTGTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCGGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTTCA TGTTTCCATG GACTTTCTAG AAGATCCATC TCAAAGGCAA 
AGAGCAATGA GCATAGCCAG CATTTTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCG 
CCATACTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC TTTTGTTGAC 
CTGGCCATCA CCATCTGCAT TGTCTTAAAC ACTCTCTTCA TGGCCATGGA GCACTATCCC 
ATGACAGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGATGGTTT TATTGTAACG CTTAGCCTGG TAGAACTTGG CCTGGCCAAC 
GTGGAAGGAT TATCAGTTCT CCGTTCATTC CGTTTGCTTC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CATTAAATAT GCTAATAAAG ATCATCGGCA ATTCAGTGGG GGCTCTGGGA 
AACCTAACCT TGGTGTTAGC CATCATCGTC TTCATTTTTG CTGTGGTTGG CATGCAGCTC 
TTTGGTAAAA GTTACAAAGA TTGTGTCTGC AAGATCAACA GTGAATGTAA GCTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGGGTGCT GTGTGGAGAG 
TGGATAGAGA CCATGTGGGA CTGCATGGAG GTCGCCGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTCAT TGGAAACCTG GTGGTCCTGA ACCTCTTTTT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCG GCCACTGATG ATGATAATGA AATGAACAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAATAGCTT ATGTGAAGAG AAAAATATAT 
GAATTTATTC AGCAGTCCTT TGTTAGAAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAGGAA AGACAATTGT ATGTCCAATC ATACAACAGA GATTGGGAAA 
GACCTTGACT ATCTTAAAGA CGTAAATGGA ACCACAAGTG GAATAGGAAC CGGCAGCAGT 
GTTGAAAAGT ACATTATTGA TGAAAGTGAT TACATGTCCT TCATAAACAA TCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACAGAGGAC 
TTCAGTAGTG AATCAGATCT GGAAGAAAGC AAAGAGAAGC TTAATGAAAG CAGTAGTTCA 
TCTGAAGGTA GCACTGTAGA CATTGGAGCA CCTGCCGAGG AACAGCCTGT GGTGGAACCC 
GAGGAAACCC TTGAACCTGA AGCCTGTTTC ACTGAAGGCT GTGTGCAAAG ATTCAAATGT 
TGTCAAATCA GTGTGGAAGA AGGGAGAGGA AAACAGTGGT GGAATCTGAG AAGAACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCAC TGGCATTTGA GGATATATAT ATTGACCAGC GAAAGACAAT CAAAACAATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTAGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGTGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC ACGATTTGAA 
GGGATGAGGG CCACATTTAA GGGATGGATG GATATAATGT ATGCAGCAGT TGATTCCAGA 
AATGTGGAAC TCCAGCCTAA GTATGAGGAA AGTCTATACA TGTATCTTTA CTTTGTTATT 
TTCATCATCT TTGGGTCCTT CTTCACCTTG AACCTGTTTA TTGGTGTCAT CATAGATAAT 
TTCAACCAGC AAAAAAAGAA GTTTGGAGGT CAAGACATCT TTATGACAGA AGAACAGAAG 
AAATACTATA ATGCAATGAA AAAATTAGGA TCAAAAAAGC CACAAAAGCC TATACCTCGA 
CCAGGAAACA AATTCCAAGG AATGGTCTTT GACTTCGTCA CCAGACAAGT TTTTGACATC 
AGCATCATGA TTCTTATCTG TCTCAACATG GTCACCATGA TGGTGGAAAC CGACGACCAG 
AGCGACTGTG TGACTAGCAT TTTGTCCCGC ATCAACTTGG TGTTCATTGT GCTGTTCACT 
GGGGAGTGTG TGCTGAAGCT CATCTCCCTC CGCCATTACT ATTTTACTAT AGGCTGGAAT 
ATCTTTGATT TTGTGGTGGT CATTCTCTCC ATAGTAGGTA TGTTTCTGGC TGAGCTGATA 
GAGAAATATT TTGTGTCCCC TACCTTGTTC CGAGTGATCC GTCTTGCCAG GATTGGCCGA 
ATCCTGCGTC TGATCAAAGG GGCTAAGGGG ATCCGCACGC TGCTCTTTGC TTTGATGATG 
TCCCTTCCTG CGTTGTTTAA CATCGGCCTC CTGCTCTTCC TGGTCATGTT CATCTATGCC 
ATCTTTGGAA TGTCCAACTT CGCCTATGTT AAGAGGGAGG TTGGAATTGA TGACATGTTT 
AACTTTGAGA CCTTTGGCAA CAGCATGATC TGCCTGTTCC AGATTACCAC CTCTGCTGGC 
TGGGATGGAT TGCTAGCACC TATTCTCAAC AGTAAACCAC CCGATTGTGA CCCTAATAAA 
GTTAACCCTG GCAGCTCAGT TAAAGGAGAC TGTGGGAACC CATCTGTTGG GATTTTCTTC 
TTTGTCAGTT ACATCATCAT ATCATTTCTA GTCGTGGTGA ACATGTATAT AGCTGTCATC 
CTGGAGAACT TCAGTGTTGC TACTGAAGAA AGCGCAGAGC CCCTGAGTGA GGATGACTTT 
GAGATGTTCT ATGAGGTTTG GGAGAAGTTT GATCCCGACG CCACCCAGTT CATGGAATTT 
GAAAAGCTAT CTCAGTTTGC AGCTGCTCTT GAACCACCGC TCAATTTGCC ACAACCGAAC 
AAACTCCAGC TCATTGCCAT GGATTTGCCC ATGGTAAGTG GTGATCGAAT CCACTGTCTT 
GACATTTTAT TTGCTTTTAC GAAGCGGGTT CTGGGGGAGA GTGGAGAGAT GGATGCCCTT 
CGAATACAGA TGGAAGAGAG ATTCATGGCC TCCAACCCAT CGAAAGTCTC CTATCAGCCA 
ATAACTACGA CTTTAAAGAG AAAACAAGAG GAAGTGTCTG CTGTTATTAT TCAGCGTGCA 
TACAGGCGCC ACCTTTTAAA GCGAACTGTA AAACAGGCAT CATTTACGTA CAACAAAAAC 
AAAATCAAGG GTGGGGCTAA TCTTCTTGTA AAAGAAGACA TGATAATTGA CAGAATAAAT 
GAAAACTCAA TTACAGAAAA AACTGATCTG ACCATGTCCA CAGCAGCTTG TCCACCCTCC 
TATGATCGGG TAACAAAACC AATTGTGGAA AAGCACGAGC AAGAAGGCAA AGATGAAAAA 
GCCAAAGGGA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001193218.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001180147.1 Bos taurus sodium channel, voltage-gated, type I, alpha subunit (SCN1A), mRNA.

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CloneID	OBa36949
Clone ID Related Accession (Same CDS sequence)	NM_001193218.1
Accession Version	NM_001193218.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-01-03
Organism	Bos taurus(cattle)
Product	sodium channel protein type 1 subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AAFC03092537.1, AAFC03092535.1, AAFC03061552.1 and AAFC03092539.1. On Jul 17, 2010 this sequence version replaced XM_874196.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00000728, SAMN02822092 [ECO:0000348] ##Evidence-Data-END##

ATGGAACAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC CGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTCTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCGGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTTCA TGTTTCCATG GACTTTCTAG AAGATCCATC TCAAAGGCAA 
AGAGCAATGA GCATAGCCAG CATTTTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCG 
CCATACTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC TTTTGTTGAC 
CTGGCCATCA CCATCTGCAT TGTCTTAAAC ACTCTCTTCA TGGCCATGGA GCACTATCCC 
ATGACAGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGATGGTTT TATTGTAACG CTTAGCCTGG TAGAACTTGG CCTGGCCAAC 
GTGGAAGGAT TATCAGTTCT CCGTTCATTC CGTTTGCTTC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CATTAAATAT GCTAATAAAG ATCATCGGCA ATTCAGTGGG GGCTCTGGGA 
AACCTAACCT TGGTGTTAGC CATCATCGTC TTCATTTTTG CTGTGGTTGG CATGCAGCTC 
TTTGGTAAAA GTTACAAAGA TTGTGTCTGC AAGATCAACA GTGAATGTAA GCTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGGGTGCT GTGTGGAGAG 
TGGATAGAGA CCATGTGGGA CTGCATGGAG GTCGCCGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTCAT TGGAAACCTG GTGGTCCTGA ACCTCTTTTT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCG GCCACTGATG ATGATAATGA AATGAACAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAATAGCTT ATGTGAAGAG AAAAATATAT 
GAATTTATTC AGCAGTCCTT TGTTAGAAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAGGAA AGACAATTGT ATGTCCAATC ATACAACAGA GATTGGGAAA 
GACCTTGACT ATCTTAAAGA CGTAAATGGA ACCACAAGTG GAATAGGAAC CGGCAGCAGT 
GTTGAAAAGT ACATTATTGA TGAAAGTGAT TACATGTCCT TCATAAACAA TCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACAGAGGAC 
TTCAGTAGTG AATCAGATCT GGAAGAAAGC AAAGAGAAGC TTAATGAAAG CAGTAGTTCA 
TCTGAAGGTA GCACTGTAGA CATTGGAGCA CCTGCCGAGG AACAGCCTGT GGTGGAACCC 
GAGGAAACCC TTGAACCTGA AGCCTGTTTC ACTGAAGGCT GTGTGCAAAG ATTCAAATGT 
TGTCAAATCA GTGTGGAAGA AGGGAGAGGA AAACAATGGT GGAATCTGAG AAGAACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCAC TGGCATTTGA GGATATATAT ATTGACCAGC GAAAGACAAT CAAAACAATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTAGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGTGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC ACGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTACTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTGTTAA CACCACAACT GGTGACATGT TTGACATCAG CGAAGTGAAT 
AATCATTCAG ATTGCCTAAT ACTAATAGAA AGAAATGAGA CTGCCCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTT 
AAGGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAGG AAAGTCTATA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAAAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCAAAAAA GCCACAAAAG CCTATACCTC GACCAGGAAA CAAATTCCAA 
GGAATGGTCT TTGACTTCGT CACCAGACAA GTTTTTGACA TCAGCATCAT GATTCTTATC 
TGTCTCAACA TGGTCACCAT GATGGTGGAA ACCGACGACC AGAGCGACTG TGTGACTAGC 
ATTTTGTCCC GCATCAACTT GGTGTTCATT GTGCTGTTCA CTGGGGAGTG TGTGCTGAAG 
CTCATCTCCC TCCGCCATTA CTATTTTACT ATAGGCTGGA ATATCTTTGA TTTTGTGGTG 
GTCATTCTCT CCATAGTAGG TATGTTTCTG GCTGAGCTGA TAGAGAAATA TTTTGTGTCC 
CCTACCTTGT TCCGAGTGAT CCGTCTTGCC AGGATTGGCC GAATCCTGCG TCTGATCAAA 
GGGGCTAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTGCTCTT CCTGGTCATG TTCATCTATG CCATCTTTGG AATGTCCAAC 
TTCGCCTATG TTAAGAGGGA GGTTGGAATT GATGACATGT TTAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTGTT CCAGATTACC ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCTATTCTCA ACAGTAAACC ACCCGATTGT GACCCTAATA AAGTTAACCC TGGCAGCTCA 
GTTAAAGGAG ACTGTGGGAA CCCATCTGTT GGGATTTTCT TCTTTGTCAG TTACATCATC 
ATATCATTTC TAGTCGTGGT GAACATGTAT ATAGCTGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGCGCAGA GCCCCTGAGT GAGGATGACT TTGAGATGTT CTACGAGGTT 
TGGGAGAAGT TTGATCCCGA CGCCACCCAG TTCATGGAAT TTGAAAAGCT ATCTCAGTTT 
GCAGCTGCTC TTGAACCACC GCTCAATTTG CCACAACCGA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTAAG TGGTGATCGA ATCCACTGTC TTGACATTTT ATTTGCTTTT 
ACGAAGCGGG TTCTGGGGGA GAGTGGAGAG ATGGATGCCC TTCGAATACA GATGGAAGAG 
AGATTCATGG CCTCCAACCC ATCGAAAGTC TCCTATCAGC CAATAACTAC GACTTTAAAG 
AGAAAACAAG AGGAAGTGTC TGCTGTTATT ATTCAGCGTG CATACAGGCG CCACCTTTTA 
AAGCGAACTG TAAAACAGGC ATCATTTACG TACAACAAAA ATAAAATCAA GGGTGGGGCT 
AATCTTCTTG TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC AATTACAGAA 
AAAACTGATC TGACCATGTC CACAGCAGCT TGTCCACCCT CCTATGATCG GGTAACAAAA 
CCAATTGTGG AAAAGCACGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001180147.1
CDS	1..5997
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDEHGPKPNSDLEAGKNLPFIYGDVPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHTVEKNITKNYNGTFVNETRNEFDWKSYIQDSRYHYFLEGFSDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAVTASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKINSECKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGIAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCMSNHTTEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVVEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDMFDISEVNNHSDCLILIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDCVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001193218.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium channel, voltage-gated, type I, alpha subunit (SCN1A), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001193218.1

ORF Insert Sequence:

ATGGAACAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC CGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTCTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCGGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTTCA TGTTTCCATG GACTTTCTAG AAGATCCATC TCAAAGGCAA 
AGAGCAATGA GCATAGCCAG CATTTTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCG 
CCATACTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC TTTTGTTGAC 
CTGGCCATCA CCATCTGCAT TGTCTTAAAC ACTCTCTTCA TGGCCATGGA GCACTATCCC 
ATGACAGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGATGGTTT TATTGTAACG CTTAGCCTGG TAGAACTTGG CCTGGCCAAC 
GTGGAAGGAT TATCAGTTCT CCGTTCATTC CGTTTGCTTC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CATTAAATAT GCTAATAAAG ATCATCGGCA ATTCAGTGGG GGCTCTGGGA 
AACCTAACCT TGGTGTTAGC CATCATCGTC TTCATTTTTG CTGTGGTTGG CATGCAGCTC 
TTTGGTAAAA GTTACAAAGA TTGTGTCTGC AAGATCAACA GTGAATGTAA GCTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGGGTGCT GTGTGGAGAG 
TGGATAGAGA CCATGTGGGA CTGCATGGAG GTCGCCGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTCAT TGGAAACCTG GTGGTCCTGA ACCTCTTTTT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCG GCCACTGATG ATGATAATGA AATGAACAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAATAGCTT ATGTGAAGAG AAAAATATAT 
GAATTTATTC AGCAGTCCTT TGTTAGAAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAGGAA AGACAATTGT ATGTCCAATC ATACAACAGA GATTGGGAAA 
GACCTTGACT ATCTTAAAGA CGTAAATGGA ACCACAAGTG GAATAGGAAC CGGCAGCAGT 
GTTGAAAAGT ACATTATTGA TGAAAGTGAT TACATGTCCT TCATAAACAA TCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACAGAGGAC 
TTCAGTAGTG AATCAGATCT GGAAGAAAGC AAAGAGAAGC TTAATGAAAG CAGTAGTTCA 
TCTGAAGGTA GCACTGTAGA CATTGGAGCA CCTGCCGAGG AACAGCCTGT GGTGGAACCC 
GAGGAAACCC TTGAACCTGA AGCCTGTTTC ACTGAAGGCT GTGTGCAAAG ATTCAAATGT 
TGTCAAATCA GTGTGGAAGA AGGGAGAGGA AAACAATGGT GGAATCTGAG AAGAACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCAC TGGCATTTGA GGATATATAT ATTGACCAGC GAAAGACAAT CAAAACAATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTAGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGTGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC ACGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTACTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTGTTAA CACCACAACT GGTGACATGT TTGACATCAG CGAAGTGAAT 
AATCATTCAG ATTGCCTAAT ACTAATAGAA AGAAATGAGA CTGCCCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTT 
AAGGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAGG AAAGTCTATA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAAAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCAAAAAA GCCACAAAAG CCTATACCTC GACCAGGAAA CAAATTCCAA 
GGAATGGTCT TTGACTTCGT CACCAGACAA GTTTTTGACA TCAGCATCAT GATTCTTATC 
TGTCTCAACA TGGTCACCAT GATGGTGGAA ACCGACGACC AGAGCGACTG TGTGACTAGC 
ATTTTGTCCC GCATCAACTT GGTGTTCATT GTGCTGTTCA CTGGGGAGTG TGTGCTGAAG 
CTCATCTCCC TCCGCCATTA CTATTTTACT ATAGGCTGGA ATATCTTTGA TTTTGTGGTG 
GTCATTCTCT CCATAGTAGG TATGTTTCTG GCTGAGCTGA TAGAGAAATA TTTTGTGTCC 
CCTACCTTGT TCCGAGTGAT CCGTCTTGCC AGGATTGGCC GAATCCTGCG TCTGATCAAA 
GGGGCTAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTGCTCTT CCTGGTCATG TTCATCTATG CCATCTTTGG AATGTCCAAC 
TTCGCCTATG TTAAGAGGGA GGTTGGAATT GATGACATGT TTAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTGTT CCAGATTACC ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCTATTCTCA ACAGTAAACC ACCCGATTGT GACCCTAATA AAGTTAACCC TGGCAGCTCA 
GTTAAAGGAG ACTGTGGGAA CCCATCTGTT GGGATTTTCT TCTTTGTCAG TTACATCATC 
ATATCATTTC TAGTCGTGGT GAACATGTAT ATAGCTGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGCGCAGA GCCCCTGAGT GAGGATGACT TTGAGATGTT CTACGAGGTT 
TGGGAGAAGT TTGATCCCGA CGCCACCCAG TTCATGGAAT TTGAAAAGCT ATCTCAGTTT 
GCAGCTGCTC TTGAACCACC GCTCAATTTG CCACAACCGA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTAAG TGGTGATCGA ATCCACTGTC TTGACATTTT ATTTGCTTTT 
ACGAAGCGGG TTCTGGGGGA GAGTGGAGAG ATGGATGCCC TTCGAATACA GATGGAAGAG 
AGATTCATGG CCTCCAACCC ATCGAAAGTC TCCTATCAGC CAATAACTAC GACTTTAAAG 
AGAAAACAAG AGGAAGTGTC TGCTGTTATT ATTCAGCGTG CATACAGGCG CCACCTTTTA 
AAGCGAACTG TAAAACAGGC ATCATTTACG TACAACAAAA ATAAAATCAA GGGTGGGGCT 
AATCTTCTTG TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC AATTACAGAA 
AAAACTGATC TGACCATGTC CACAGCAGCT TGTCCACCCT CCTATGATCG GGTAACAAAA 
CCAATTGTGG AAAAGCACGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_015475298.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330784.1 Bos taurus sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X3, mRNA.

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CloneID	OBa16416
Clone ID Related Accession (Same CDS sequence)	XM_015475298.1
Accession Version	XM_015475298.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sodium channel protein type 1 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC CGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTGTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCGGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTTCA TGTTTCCATG GACTTTCTAG AAGATCCATC TCAAAGGCAA 
AGAGCAATGA GCATAGCCAG CATTTTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCG 
CCATACTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC TTTTGTTGAC 
CTGGCCATCA CCATCTGCAT TGTCTTAAAC ACTCTCTTCA TGGCCATGGA GCACTATCCC 
ATGACAGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGATGGTTT TATTGTAACG CTTAGCCTGG TAGAACTTGG CCTGGCCAAC 
GTGGAAGGAT TATCAGTTCT CCGTTCATTC CGTTTGCTTC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CATTAAATAT GCTAATAAAG ATCATCGGCA ATTCAGTGGG GGCTCTGGGA 
AACCTAACCT TGGTGTTAGC CATCATCGTC TTCATTTTTG CTGTGGTTGG CATGCAGCTC 
TTTGGTAAAA GTTACAAAGA TTGTGTCTGC AAGATCAACA GTGAGTGTAA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGGGTGCT GTGTGGAGAG 
TGGATAGAGA CCATGTGGGA CTGCATGGAG GTCGCCGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTCAT TGGAAACCTG GTGGTCCTGA ACCTCTTTTT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCG GCCACTGATG ATGATAATGA AATGAACAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAATAGCTT ATGTGAAGAG AAAAATATAT 
GAATTTATTC AGCAGTCCTT TGTTAGAAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAGGAA AGACAATTGT ATGTCCAATC ATACAACAGA GATTGGGAAA 
GACCTTGACT ATCTTAAAGA CGTAAATGGA ACCACAAGTG GAATAGGAAC CGGCAGCAGT 
GTTGAAAAGT ACATTATTGA TGAAAGTGAT TACATGTCCT TCATAAACAA TCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACAGAGGAC 
TTCAGTAGTG AATCAGATCT GGAAGAAAGC AAAGAGAAGC TTAATGAAAG CAGTAGTTCA 
TCTGAAGGTA GCACTGTAGA CATTGGAGCA CCTGCCGAGG AACAGCCTGT GGTGGAACCC 
GAGGAAACCC TTGAACCTGA AGCCTGTTTC ACTGAAGGCT GTGTGCAAAG ATTCAAATGT 
TGTCAAATCA GTGTGGAAGA AGGGAGAGGA AAACAGTGGT GGAATCTGAG AAGAACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCAC TGGCATTTGA GGATATATAT ATTGACCAGC GAAAGACAAT CAAAACAATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTAGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGTGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC ACGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTACTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTGTTAA CACCACAACT GGTGACATGT TTGACATCAG CGAAGTGAAT 
AATCATTCAG ATTGCCTAAT ACTAATAGAA AGAAATGAGA CTGCCCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTT 
AAGGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAGG AAAGTCTATA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAAAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCAAAAAA GCCACAAAAG CCTATACCTC GACCAGGAAA CAAATTCCAA 
GGAATGGTCT TTGACTTCGT CACCAGACAA GTTTTTGACA TCAGCATCAT GATTCTTATC 
TGTCTCAACA TGGTCACCAT GATGGTGGAA ACCGACGACC AGAGCGACTG TGTGACTAGC 
ATTTTGTCCC GCATCAACTT GGTGTTCATT GTGCTGTTCA CTGGGGAGTG TGTGCTGAAG 
CTCATCTCCC TCCGCCATTA CTATTTTACT ATAGGCTGGA ATATCTTTGA TTTTGTGGTG 
GTCATTCTCT CCATAGTAGG TATGTTTCTG GCTGAGCTGA TAGAGAAATA TTTTGTGTCC 
CCTACCTTGT TCCGAGTGAT CCGTCTTGCC AGGATTGGCC GAATCCTGCG TCTGATCAAA 
GGGGCTAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTGCTCTT CCTGGTCATG TTCATCTATG CCATCTTTGG AATGTCCAAC 
TTCGCCTATG TTAAGAGGGA GGTTGGAATT GATGACATGT TTAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTGTT CCAGATTACC ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCTATTCTCA ACAGTAAACC ACCCGATTGT GACCCTAATA AAGTTAACCC TGGCAGCTCA 
GTTAAAGGAG ACTGTGGGAA CCCATCTGTT GGGATTTTCT TCTTTGTCAG TTACATCATC 
ATATCATTTC TAGTCGTGGT GAACATGTAT ATAGCTGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGCGCAGA GCCCCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA CGCCACCCAG TTCATGGAAT TTGAAAAGCT ATCTCAGTTT 
GCAGCTGCTC TTGAACCACC GCTCAATTTG CCACAACCGA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTAAG TGGTGATCGA ATCCACTGTC TTGACATTTT ATTTGCTTTT 
ACGAAGCGGG TTCTGGGGGA GAGTGGAGAG ATGGATGCCC TTCGAATACA GATGGAAGAG 
AGATTCATGG CCTCCAACCC ATCGAAAGTC TCCTATCAGC CAATAACTAC GACTTTAAAG 
AGAAAACAAG AGGAAGTGTC TGCTGTTATT ATTCAGCGTG CATACAGGCG CCACCTTTTA 
AAGCGAACTG TAAAACAGGC ATCATTTACG TACAACAAAA ACAAAATCAA GGGTGGGGCT 
AATCTTCTTG TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC AATTACAGAA 
AAAACTGATC TGACCATGTC CACAGCAGCT TGTCCACCCT CCTATGATCG GGTAACAAAA 
CCAATTGTGG AAAAGCACGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330784.1
CDS	165..6161
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDEHGPKPNSDLEAGKNLPFIYGDVPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHTVEKNITKNYNGTFVNETRNEFDWKSYIQDSRYHYFLEGFSDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAVTASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKINSECKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGIAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCMSNHTTEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVVEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDMFDISEVNNHSDCLILIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDCVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_015475298.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475298.1

ORF Insert Sequence:

ATGGAACAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC CGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTGTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCGGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTTCA TGTTTCCATG GACTTTCTAG AAGATCCATC TCAAAGGCAA 
AGAGCAATGA GCATAGCCAG CATTTTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCG 
CCATACTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC TTTTGTTGAC 
CTGGCCATCA CCATCTGCAT TGTCTTAAAC ACTCTCTTCA TGGCCATGGA GCACTATCCC 
ATGACAGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGATGGTTT TATTGTAACG CTTAGCCTGG TAGAACTTGG CCTGGCCAAC 
GTGGAAGGAT TATCAGTTCT CCGTTCATTC CGTTTGCTTC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CATTAAATAT GCTAATAAAG ATCATCGGCA ATTCAGTGGG GGCTCTGGGA 
AACCTAACCT TGGTGTTAGC CATCATCGTC TTCATTTTTG CTGTGGTTGG CATGCAGCTC 
TTTGGTAAAA GTTACAAAGA TTGTGTCTGC AAGATCAACA GTGAGTGTAA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGGGTGCT GTGTGGAGAG 
TGGATAGAGA CCATGTGGGA CTGCATGGAG GTCGCCGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTCAT TGGAAACCTG GTGGTCCTGA ACCTCTTTTT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCG GCCACTGATG ATGATAATGA AATGAACAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAATAGCTT ATGTGAAGAG AAAAATATAT 
GAATTTATTC AGCAGTCCTT TGTTAGAAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAGGAA AGACAATTGT ATGTCCAATC ATACAACAGA GATTGGGAAA 
GACCTTGACT ATCTTAAAGA CGTAAATGGA ACCACAAGTG GAATAGGAAC CGGCAGCAGT 
GTTGAAAAGT ACATTATTGA TGAAAGTGAT TACATGTCCT TCATAAACAA TCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACAGAGGAC 
TTCAGTAGTG AATCAGATCT GGAAGAAAGC AAAGAGAAGC TTAATGAAAG CAGTAGTTCA 
TCTGAAGGTA GCACTGTAGA CATTGGAGCA CCTGCCGAGG AACAGCCTGT GGTGGAACCC 
GAGGAAACCC TTGAACCTGA AGCCTGTTTC ACTGAAGGCT GTGTGCAAAG ATTCAAATGT 
TGTCAAATCA GTGTGGAAGA AGGGAGAGGA AAACAGTGGT GGAATCTGAG AAGAACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCAC TGGCATTTGA GGATATATAT ATTGACCAGC GAAAGACAAT CAAAACAATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTAGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGTGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC ACGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTACTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTGTTAA CACCACAACT GGTGACATGT TTGACATCAG CGAAGTGAAT 
AATCATTCAG ATTGCCTAAT ACTAATAGAA AGAAATGAGA CTGCCCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTT 
AAGGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAGG AAAGTCTATA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAAAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCAAAAAA GCCACAAAAG CCTATACCTC GACCAGGAAA CAAATTCCAA 
GGAATGGTCT TTGACTTCGT CACCAGACAA GTTTTTGACA TCAGCATCAT GATTCTTATC 
TGTCTCAACA TGGTCACCAT GATGGTGGAA ACCGACGACC AGAGCGACTG TGTGACTAGC 
ATTTTGTCCC GCATCAACTT GGTGTTCATT GTGCTGTTCA CTGGGGAGTG TGTGCTGAAG 
CTCATCTCCC TCCGCCATTA CTATTTTACT ATAGGCTGGA ATATCTTTGA TTTTGTGGTG 
GTCATTCTCT CCATAGTAGG TATGTTTCTG GCTGAGCTGA TAGAGAAATA TTTTGTGTCC 
CCTACCTTGT TCCGAGTGAT CCGTCTTGCC AGGATTGGCC GAATCCTGCG TCTGATCAAA 
GGGGCTAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTGCTCTT CCTGGTCATG TTCATCTATG CCATCTTTGG AATGTCCAAC 
TTCGCCTATG TTAAGAGGGA GGTTGGAATT GATGACATGT TTAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTGTT CCAGATTACC ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCTATTCTCA ACAGTAAACC ACCCGATTGT GACCCTAATA AAGTTAACCC TGGCAGCTCA 
GTTAAAGGAG ACTGTGGGAA CCCATCTGTT GGGATTTTCT TCTTTGTCAG TTACATCATC 
ATATCATTTC TAGTCGTGGT GAACATGTAT ATAGCTGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGCGCAGA GCCCCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA CGCCACCCAG TTCATGGAAT TTGAAAAGCT ATCTCAGTTT 
GCAGCTGCTC TTGAACCACC GCTCAATTTG CCACAACCGA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTAAG TGGTGATCGA ATCCACTGTC TTGACATTTT ATTTGCTTTT 
ACGAAGCGGG TTCTGGGGGA GAGTGGAGAG ATGGATGCCC TTCGAATACA GATGGAAGAG 
AGATTCATGG CCTCCAACCC ATCGAAAGTC TCCTATCAGC CAATAACTAC GACTTTAAAG 
AGAAAACAAG AGGAAGTGTC TGCTGTTATT ATTCAGCGTG CATACAGGCG CCACCTTTTA 
AAGCGAACTG TAAAACAGGC ATCATTTACG TACAACAAAA ACAAAATCAA GGGTGGGGCT 
AATCTTCTTG TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC AATTACAGAA 
AAAACTGATC TGACCATGTC CACAGCAGCT TGTCCACCCT CCTATGATCG GGTAACAAAA 
CCAATTGTGG AAAAGCACGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_015475302.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015330788.1 Bos taurus sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X4, mRNA.

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CloneID	OBa16417
Clone ID Related Accession (Same CDS sequence)	XM_015475302.1
Accession Version	XM_015475302.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sodium channel protein type 1 subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC CGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTGTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTTCAT GTTTCCATGG ACTTTCTAGA AGATCCATCT 
CAAAGGCAAA GAGCAATGAG CATAGCCAGC ATTTTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCGC CATACTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCT 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCTTAAACA CTCTCTTCAT GGCCATGGAG 
CACTATCCCA TGACAGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGATGGTTTT ATTGTAACGC TTAGCCTGGT AGAACTTGGC 
CTGGCCAACG TGGAAGGATT ATCAGTTCTC CGTTCATTCC GTTTGCTTCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC ATTAAATATG CTAATAAAGA TCATCGGCAA TTCAGTGGGG 
GCTCTGGGAA ACCTAACCTT GGTGTTAGCC ATCATCGTCT TCATTTTTGC TGTGGTTGGC 
ATGCAGCTCT TTGGTAAAAG TTACAAAGAT TGTGTCTGCA AGATCAACAG TGAGTGTAAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGGGTGCTG 
TGTGGAGAGT GGATAGAGAC CATGTGGGAC TGCATGGAGG TCGCCGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTCATT GGAAACCTGG TGGTCCTGAA CCTCTTTTTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCGG CCACTGATGA TGATAATGAA 
ATGAACAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAATAGCTTA TGTGAAGAGA 
AAAATATATG AATTTATTCA GCAGTCCTTT GTTAGAAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAGGAAA GACAATTGTA TGTCCAATCA TACAACAGAG 
ATTGGGAAAG ACCTTGACTA TCTTAAAGAC GTAAATGGAA CCACAAGTGG AATAGGAACC 
GGCAGCAGTG TTGAAAAGTA CATTATTGAT GAAAGTGATT ACATGTCCTT CATAAACAAT 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACAGAGGACT TCAGTAGTGA ATCAGATCTG GAAGAAAGCA AAGAGAAGCT TAATGAAAGC 
AGTAGTTCAT CTGAAGGTAG CACTGTAGAC ATTGGAGCAC CTGCCGAGGA ACAGCCTGTG 
GTGGAACCCG AGGAAACCCT TGAACCTGAA GCCTGTTTCA CTGAAGGCTG TGTGCAAAGA 
TTCAAATGTT GTCAAATCAG TGTGGAAGAA GGGAGAGGAA AACAGTGGTG GAATCTGAGA 
AGAACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCACT GGCATTTGAG GATATATATA TTGACCAGCG AAAGACAATC 
AAAACAATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTAGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGTGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCA 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTACTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTGTTAAC ACCACAACTG GTGACATGTT TGACATCAGC 
GAAGTGAATA ATCATTCAGA TTGCCTAATA CTAATAGAAA GAAATGAGAC TGCCCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTTA AGGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAGGA AAGTCTATAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAAAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCAAAAAAG CCACAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTCCAAG GAATGGTCTT TGACTTCGTC ACCAGACAAG TTTTTGACAT CAGCATCATG 
ATTCTTATCT GTCTCAACAT GGTCACCATG ATGGTGGAAA CCGACGACCA GAGCGACTGT 
GTGACTAGCA TTTTGTCCCG CATCAACTTG GTGTTCATTG TGCTGTTCAC TGGGGAGTGT 
GTGCTGAAGC TCATCTCCCT CCGCCATTAC TATTTTACTA TAGGCTGGAA TATCTTTGAT 
TTTGTGGTGG TCATTCTCTC CATAGTAGGT ATGTTTCTGG CTGAGCTGAT AGAGAAATAT 
TTTGTGTCCC CTACCTTGTT CCGAGTGATC CGTCTTGCCA GGATTGGCCG AATCCTGCGT 
CTGATCAAAG GGGCTAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTGCTCTTC CTGGTCATGT TCATCTATGC CATCTTTGGA 
ATGTCCAACT TCGCCTATGT TAAGAGGGAG GTTGGAATTG ATGACATGTT TAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTGTTC CAGATTACCA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CTATTCTCAA CAGTAAACCA CCCGATTGTG ACCCTAATAA AGTTAACCCT 
GGCAGCTCAG TTAAAGGAGA CTGTGGGAAC CCATCTGTTG GGATTTTCTT CTTTGTCAGT 
TACATCATCA TATCATTTCT AGTCGTGGTG AACATGTATA TAGCTGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGCGCAGAG CCCCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAC GCCACCCAGT TCATGGAATT TGAAAAGCTA 
TCTCAGTTTG CAGCTGCTCT TGAACCACCG CTCAATTTGC CACAACCGAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTAAGT GGTGATCGAA TCCACTGTCT TGACATTTTA 
TTTGCTTTTA CGAAGCGGGT TCTGGGGGAG AGTGGAGAGA TGGATGCCCT TCGAATACAG 
ATGGAAGAGA GATTCATGGC CTCCAACCCA TCGAAAGTCT CCTATCAGCC AATAACTACG 
ACTTTAAAGA GAAAACAAGA GGAAGTGTCT GCTGTTATTA TTCAGCGTGC ATACAGGCGC 
CACCTTTTAA AGCGAACTGT AAAACAGGCA TCATTTACGT ACAACAAAAA CAAAATCAAG 
GGTGGGGCTA ATCTTCTTGT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCA 
ATTACAGAAA AAACTGATCT GACCATGTCC ACAGCAGCTT GTCCACCCTC CTATGATCGG 
GTAACAAAAC CAATTGTGGA AAAGCACGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015330788.1
CDS	165..6110
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDEHGPKPNSDLEAGKNLPFIYGDVPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHTVEKNITKNYNGTFVNETRNEFDWKSYIQDSRYHYFLEGFSDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAVTASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKINSECKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGIAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCMSNHTTEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVVEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDMFDISEVNNHSDCLILIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDCVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_015475302.1
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015475302.1

ORF Insert Sequence:

ATGGAACAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC CGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTGTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTTCAT GTTTCCATGG ACTTTCTAGA AGATCCATCT 
CAAAGGCAAA GAGCAATGAG CATAGCCAGC ATTTTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCGC CATACTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCT 
TTTGTTGACC TGGCCATCAC CATCTGCATT GTCTTAAACA CTCTCTTCAT GGCCATGGAG 
CACTATCCCA TGACAGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGATGGTTTT ATTGTAACGC TTAGCCTGGT AGAACTTGGC 
CTGGCCAACG TGGAAGGATT ATCAGTTCTC CGTTCATTCC GTTTGCTTCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC ATTAAATATG CTAATAAAGA TCATCGGCAA TTCAGTGGGG 
GCTCTGGGAA ACCTAACCTT GGTGTTAGCC ATCATCGTCT TCATTTTTGC TGTGGTTGGC 
ATGCAGCTCT TTGGTAAAAG TTACAAAGAT TGTGTCTGCA AGATCAACAG TGAGTGTAAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGGGTGCTG 
TGTGGAGAGT GGATAGAGAC CATGTGGGAC TGCATGGAGG TCGCCGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTCATT GGAAACCTGG TGGTCCTGAA CCTCTTTTTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCGG CCACTGATGA TGATAATGAA 
ATGAACAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAATAGCTTA TGTGAAGAGA 
AAAATATATG AATTTATTCA GCAGTCCTTT GTTAGAAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAGGAAA GACAATTGTA TGTCCAATCA TACAACAGAG 
ATTGGGAAAG ACCTTGACTA TCTTAAAGAC GTAAATGGAA CCACAAGTGG AATAGGAACC 
GGCAGCAGTG TTGAAAAGTA CATTATTGAT GAAAGTGATT ACATGTCCTT CATAAACAAT 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACAGAGGACT TCAGTAGTGA ATCAGATCTG GAAGAAAGCA AAGAGAAGCT TAATGAAAGC 
AGTAGTTCAT CTGAAGGTAG CACTGTAGAC ATTGGAGCAC CTGCCGAGGA ACAGCCTGTG 
GTGGAACCCG AGGAAACCCT TGAACCTGAA GCCTGTTTCA CTGAAGGCTG TGTGCAAAGA 
TTCAAATGTT GTCAAATCAG TGTGGAAGAA GGGAGAGGAA AACAGTGGTG GAATCTGAGA 
AGAACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCACT GGCATTTGAG GATATATATA TTGACCAGCG AAAGACAATC 
AAAACAATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTAGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGTGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCA 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTACTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTGTTAAC ACCACAACTG GTGACATGTT TGACATCAGC 
GAAGTGAATA ATCATTCAGA TTGCCTAATA CTAATAGAAA GAAATGAGAC TGCCCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTTA AGGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAGGA AAGTCTATAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAAAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCAAAAAAG CCACAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTCCAAG GAATGGTCTT TGACTTCGTC ACCAGACAAG TTTTTGACAT CAGCATCATG 
ATTCTTATCT GTCTCAACAT GGTCACCATG ATGGTGGAAA CCGACGACCA GAGCGACTGT 
GTGACTAGCA TTTTGTCCCG CATCAACTTG GTGTTCATTG TGCTGTTCAC TGGGGAGTGT 
GTGCTGAAGC TCATCTCCCT CCGCCATTAC TATTTTACTA TAGGCTGGAA TATCTTTGAT 
TTTGTGGTGG TCATTCTCTC CATAGTAGGT ATGTTTCTGG CTGAGCTGAT AGAGAAATAT 
TTTGTGTCCC CTACCTTGTT CCGAGTGATC CGTCTTGCCA GGATTGGCCG AATCCTGCGT 
CTGATCAAAG GGGCTAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTGCTCTTC CTGGTCATGT TCATCTATGC CATCTTTGGA 
ATGTCCAACT TCGCCTATGT TAAGAGGGAG GTTGGAATTG ATGACATGTT TAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTGTTC CAGATTACCA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CTATTCTCAA CAGTAAACCA CCCGATTGTG ACCCTAATAA AGTTAACCCT 
GGCAGCTCAG TTAAAGGAGA CTGTGGGAAC CCATCTGTTG GGATTTTCTT CTTTGTCAGT 
TACATCATCA TATCATTTCT AGTCGTGGTG AACATGTATA TAGCTGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGCGCAGAG CCCCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAC GCCACCCAGT TCATGGAATT TGAAAAGCTA 
TCTCAGTTTG CAGCTGCTCT TGAACCACCG CTCAATTTGC CACAACCGAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTAAGT GGTGATCGAA TCCACTGTCT TGACATTTTA 
TTTGCTTTTA CGAAGCGGGT TCTGGGGGAG AGTGGAGAGA TGGATGCCCT TCGAATACAG 
ATGGAAGAGA GATTCATGGC CTCCAACCCA TCGAAAGTCT CCTATCAGCC AATAACTACG 
ACTTTAAAGA GAAAACAAGA GGAAGTGTCT GCTGTTATTA TTCAGCGTGC ATACAGGCGC 
CACCTTTTAA AGCGAACTGT AAAACAGGCA TCATTTACGT ACAACAAAAA CAAAATCAAG 
GGTGGGGCTA ATCTTCTTGT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCA 
ATTACAGAAA AAACTGATCT GACCATGTCC ACAGCAGCTT GTCCACCCTC CTATGATCGG 
GTAACAAAAC CAATTGTGGA AAAGCACGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_010801984.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010800286.1 Bos taurus sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X2, mRNA.

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CloneID	OBa16415
Clone ID Related Accession (Same CDS sequence)	XM_010801984.2 , XM_005202445.3
Accession Version	XM_010801984.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sodium channel protein type 1 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010801984.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC CGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTGTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCGGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT TCATGTTTCC 
ATGGACTTTC TAGAAGATCC ATCTCAAAGG CAAAGAGCAA TGAGCATAGC CAGCATTTTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCGCCATACT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCTTTTGTT GACCTGGCCA TCACCATCTG CATTGTCTTA 
AACACTCTCT TCATGGCCAT GGAGCACTAT CCCATGACAG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGATGG TTTTATTGTA 
ACGCTTAGCC TGGTAGAACT TGGCCTGGCC AACGTGGAAG GATTATCAGT TCTCCGTTCA 
TTCCGTTTGC TTCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACATTAAA TATGCTAATA 
AAGATCATCG GCAATTCAGT GGGGGCTCTG GGAAACCTAA CCTTGGTGTT AGCCATCATC 
GTCTTCATTT TTGCTGTGGT TGGCATGCAG CTCTTTGGTA AAAGTTACAA AGATTGTGTC 
TGCAAGATCA ACAGTGAGTG TAAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGGGT GCTGTGTGGA GAGTGGATAG AGACCATGTG GGACTGCATG 
GAGGTCGCCG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT CATTGGAAAC 
CTGGTGGTCC TGAACCTCTT TTTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCGGCCACTG ATGATGATAA TGAAATGAAC AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAATAG CTTATGTGAA GAGAAAAATA TATGAATTTA TTCAGCAGTC CTTTGTTAGA 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAG GAAAGACAAT 
TGTATGTCCA ATCATACAAC AGAGATTGGG AAAGACCTTG ACTATCTTAA AGACGTAAAT 
GGAACCACAA GTGGAATAGG AACCGGCAGC AGTGTTGAAA AGTACATTAT TGATGAAAGT 
GATTACATGT CCTTCATAAA CAATCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACAGAG GACTTCAGTA GTGAATCAGA TCTGGAAGAA 
AGCAAAGAGA AGCTTAATGA AAGCAGTAGT TCATCTGAAG GTAGCACTGT AGACATTGGA 
GCACCTGCCG AGGAACAGCC TGTGGTGGAA CCCGAGGAAA CCCTTGAACC TGAAGCCTGT 
TTCACTGAAG GCTGTGTGCA AAGATTCAAA TGTTGTCAAA TCAGTGTGGA AGAAGGGAGA 
GGAAAACAGT GGTGGAATCT GAGAAGAACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CACTGGCATT TGAGGATATA 
TATATTGACC AGCGAAAGAC AATCAAAACA ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT AGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGT GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCACGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTA CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTGT TAACACCACA 
ACTGGTGACA TGTTTGACAT CAGCGAAGTG AATAATCATT CAGATTGCCT AATACTAATA 
GAAAGAAATG AGACTGCCCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTTAAGGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AGGAAAGTCT ATACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAAAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCAAA AAAGCCACAA 
AAGCCTATAC CTCGACCAGG AAACAAATTC CAAGGAATGG TCTTTGACTT CGTCACCAGA 
CAAGTTTTTG ACATCAGCAT CATGATTCTT ATCTGTCTCA ACATGGTCAC CATGATGGTG 
GAAACCGACG ACCAGAGCGA CTGTGTGACT AGCATTTTGT CCCGCATCAA CTTGGTGTTC 
ATTGTGCTGT TCACTGGGGA GTGTGTGCTG AAGCTCATCT CCCTCCGCCA TTACTATTTT 
ACTATAGGCT GGAATATCTT TGATTTTGTG GTGGTCATTC TCTCCATAGT AGGTATGTTT 
CTGGCTGAGC TGATAGAGAA ATATTTTGTG TCCCCTACCT TGTTCCGAGT GATCCGTCTT 
GCCAGGATTG GCCGAATCCT GCGTCTGATC AAAGGGGCTA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTGCT CTTCCTGGTC 
ATGTTCATCT ATGCCATCTT TGGAATGTCC AACTTCGCCT ATGTTAAGAG GGAGGTTGGA 
ATTGATGACA TGTTTAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT GTTCCAGATT 
ACCACCTCTG CTGGCTGGGA TGGATTGCTA GCACCTATTC TCAACAGTAA ACCACCCGAT 
TGTGACCCTA ATAAAGTTAA CCCTGGCAGC TCAGTTAAAG GAGACTGTGG GAACCCATCT 
GTTGGGATTT TCTTCTTTGT CAGTTACATC ATCATATCAT TTCTAGTCGT GGTGAACATG 
TATATAGCTG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGCGC AGAGCCCCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGACGCCACC 
CAGTTCATGG AATTTGAAAA GCTATCTCAG TTTGCAGCTG CTCTTGAACC ACCGCTCAAT 
TTGCCACAAC CGAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT AAGTGGTGAT 
CGAATCCACT GTCTTGACAT TTTATTTGCT TTTACGAAGC GGGTTCTGGG GGAGAGTGGA 
GAGATGGATG CCCTTCGAAT ACAGATGGAA GAGAGATTCA TGGCCTCCAA CCCATCGAAA 
GTCTCCTATC AGCCAATAAC TACGACTTTA AAGAGAAAAC AAGAGGAAGT GTCTGCTGTT 
ATTATTCAGC GTGCATACAG GCGCCACCTT TTAAAGCGAA CTGTAAAACA GGCATCATTT 
ACGTACAACA AAAACAAAAT CAAGGGTGGG GCTAATCTTC TTGTAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCAATTACA GAAAAAACTG ATCTGACCAT GTCCACAGCA 
GCTTGTCCAC CCTCCTATGA TCGGGTAACA AAACCAATTG TGGAAAAGCA CGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010800286.1
CDS	304..6333
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDEHGPKPNSDLEAGKNLPFIYGDVPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHTVEKNITKNYNGTFVNETRNEFDWKSYIQDSRYHYFLEGFSDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAVTASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKINSECKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGIAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCMSNHTTEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVVEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDMFDISEVNNHSDCLILIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDCVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_010801984.2
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010801984.2

ORF Insert Sequence:

ATGGAACAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC CGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTGTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCGGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT TCATGTTTCC 
ATGGACTTTC TAGAAGATCC ATCTCAAAGG CAAAGAGCAA TGAGCATAGC CAGCATTTTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCGCCATACT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCTTTTGTT GACCTGGCCA TCACCATCTG CATTGTCTTA 
AACACTCTCT TCATGGCCAT GGAGCACTAT CCCATGACAG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGATGG TTTTATTGTA 
ACGCTTAGCC TGGTAGAACT TGGCCTGGCC AACGTGGAAG GATTATCAGT TCTCCGTTCA 
TTCCGTTTGC TTCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACATTAAA TATGCTAATA 
AAGATCATCG GCAATTCAGT GGGGGCTCTG GGAAACCTAA CCTTGGTGTT AGCCATCATC 
GTCTTCATTT TTGCTGTGGT TGGCATGCAG CTCTTTGGTA AAAGTTACAA AGATTGTGTC 
TGCAAGATCA ACAGTGAGTG TAAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGGGT GCTGTGTGGA GAGTGGATAG AGACCATGTG GGACTGCATG 
GAGGTCGCCG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT CATTGGAAAC 
CTGGTGGTCC TGAACCTCTT TTTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCGGCCACTG ATGATGATAA TGAAATGAAC AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAATAG CTTATGTGAA GAGAAAAATA TATGAATTTA TTCAGCAGTC CTTTGTTAGA 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAG GAAAGACAAT 
TGTATGTCCA ATCATACAAC AGAGATTGGG AAAGACCTTG ACTATCTTAA AGACGTAAAT 
GGAACCACAA GTGGAATAGG AACCGGCAGC AGTGTTGAAA AGTACATTAT TGATGAAAGT 
GATTACATGT CCTTCATAAA CAATCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACAGAG GACTTCAGTA GTGAATCAGA TCTGGAAGAA 
AGCAAAGAGA AGCTTAATGA AAGCAGTAGT TCATCTGAAG GTAGCACTGT AGACATTGGA 
GCACCTGCCG AGGAACAGCC TGTGGTGGAA CCCGAGGAAA CCCTTGAACC TGAAGCCTGT 
TTCACTGAAG GCTGTGTGCA AAGATTCAAA TGTTGTCAAA TCAGTGTGGA AGAAGGGAGA 
GGAAAACAGT GGTGGAATCT GAGAAGAACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CACTGGCATT TGAGGATATA 
TATATTGACC AGCGAAAGAC AATCAAAACA ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT AGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGT GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCACGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTA CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTGT TAACACCACA 
ACTGGTGACA TGTTTGACAT CAGCGAAGTG AATAATCATT CAGATTGCCT AATACTAATA 
GAAAGAAATG AGACTGCCCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTTAAGGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AGGAAAGTCT ATACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAAAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCAAA AAAGCCACAA 
AAGCCTATAC CTCGACCAGG AAACAAATTC CAAGGAATGG TCTTTGACTT CGTCACCAGA 
CAAGTTTTTG ACATCAGCAT CATGATTCTT ATCTGTCTCA ACATGGTCAC CATGATGGTG 
GAAACCGACG ACCAGAGCGA CTGTGTGACT AGCATTTTGT CCCGCATCAA CTTGGTGTTC 
ATTGTGCTGT TCACTGGGGA GTGTGTGCTG AAGCTCATCT CCCTCCGCCA TTACTATTTT 
ACTATAGGCT GGAATATCTT TGATTTTGTG GTGGTCATTC TCTCCATAGT AGGTATGTTT 
CTGGCTGAGC TGATAGAGAA ATATTTTGTG TCCCCTACCT TGTTCCGAGT GATCCGTCTT 
GCCAGGATTG GCCGAATCCT GCGTCTGATC AAAGGGGCTA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTGCT CTTCCTGGTC 
ATGTTCATCT ATGCCATCTT TGGAATGTCC AACTTCGCCT ATGTTAAGAG GGAGGTTGGA 
ATTGATGACA TGTTTAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT GTTCCAGATT 
ACCACCTCTG CTGGCTGGGA TGGATTGCTA GCACCTATTC TCAACAGTAA ACCACCCGAT 
TGTGACCCTA ATAAAGTTAA CCCTGGCAGC TCAGTTAAAG GAGACTGTGG GAACCCATCT 
GTTGGGATTT TCTTCTTTGT CAGTTACATC ATCATATCAT TTCTAGTCGT GGTGAACATG 
TATATAGCTG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGCGC AGAGCCCCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGACGCCACC 
CAGTTCATGG AATTTGAAAA GCTATCTCAG TTTGCAGCTG CTCTTGAACC ACCGCTCAAT 
TTGCCACAAC CGAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT AAGTGGTGAT 
CGAATCCACT GTCTTGACAT TTTATTTGCT TTTACGAAGC GGGTTCTGGG GGAGAGTGGA 
GAGATGGATG CCCTTCGAAT ACAGATGGAA GAGAGATTCA TGGCCTCCAA CCCATCGAAA 
GTCTCCTATC AGCCAATAAC TACGACTTTA AAGAGAAAAC AAGAGGAAGT GTCTGCTGTT 
ATTATTCAGC GTGCATACAG GCGCCACCTT TTAAAGCGAA CTGTAAAACA GGCATCATTT 
ACGTACAACA AAAACAAAAT CAAGGGTGGG GCTAATCTTC TTGTAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCAATTACA GAAAAAACTG ATCTGACCAT GTCCACAGCA 
GCTTGTCCAC CCTCCTATGA TCGGGTAACA AAACCAATTG TGGAAAAGCA CGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_005202445.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005202502.1 Bos taurus sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X1, mRNA.

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CloneID	OBa16415
Clone ID Related Accession (Same CDS sequence)	XM_010801984.2 , XM_005202445.3
Accession Version	XM_005202445.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	sodium channel protein type 1 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103838.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005202445.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC CGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTGTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCGGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT TCATGTTTCC 
ATGGACTTTC TAGAAGATCC ATCTCAAAGG CAAAGAGCAA TGAGCATAGC CAGCATTTTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCGCCATACT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCTTTTGTT GACCTGGCCA TCACCATCTG CATTGTCTTA 
AACACTCTCT TCATGGCCAT GGAGCACTAT CCCATGACAG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGATGG TTTTATTGTA 
ACGCTTAGCC TGGTAGAACT TGGCCTGGCC AACGTGGAAG GATTATCAGT TCTCCGTTCA 
TTCCGTTTGC TTCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACATTAAA TATGCTAATA 
AAGATCATCG GCAATTCAGT GGGGGCTCTG GGAAACCTAA CCTTGGTGTT AGCCATCATC 
GTCTTCATTT TTGCTGTGGT TGGCATGCAG CTCTTTGGTA AAAGTTACAA AGATTGTGTC 
TGCAAGATCA ACAGTGAGTG TAAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGGGT GCTGTGTGGA GAGTGGATAG AGACCATGTG GGACTGCATG 
GAGGTCGCCG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT CATTGGAAAC 
CTGGTGGTCC TGAACCTCTT TTTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCGGCCACTG ATGATGATAA TGAAATGAAC AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAATAG CTTATGTGAA GAGAAAAATA TATGAATTTA TTCAGCAGTC CTTTGTTAGA 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAG GAAAGACAAT 
TGTATGTCCA ATCATACAAC AGAGATTGGG AAAGACCTTG ACTATCTTAA AGACGTAAAT 
GGAACCACAA GTGGAATAGG AACCGGCAGC AGTGTTGAAA AGTACATTAT TGATGAAAGT 
GATTACATGT CCTTCATAAA CAATCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACAGAG GACTTCAGTA GTGAATCAGA TCTGGAAGAA 
AGCAAAGAGA AGCTTAATGA AAGCAGTAGT TCATCTGAAG GTAGCACTGT AGACATTGGA 
GCACCTGCCG AGGAACAGCC TGTGGTGGAA CCCGAGGAAA CCCTTGAACC TGAAGCCTGT 
TTCACTGAAG GCTGTGTGCA AAGATTCAAA TGTTGTCAAA TCAGTGTGGA AGAAGGGAGA 
GGAAAACAGT GGTGGAATCT GAGAAGAACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CACTGGCATT TGAGGATATA 
TATATTGACC AGCGAAAGAC AATCAAAACA ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT AGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGT GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCACGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTA CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTGT TAACACCACA 
ACTGGTGACA TGTTTGACAT CAGCGAAGTG AATAATCATT CAGATTGCCT AATACTAATA 
GAAAGAAATG AGACTGCCCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTTAAGGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AGGAAAGTCT ATACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAAAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCAAA AAAGCCACAA 
AAGCCTATAC CTCGACCAGG AAACAAATTC CAAGGAATGG TCTTTGACTT CGTCACCAGA 
CAAGTTTTTG ACATCAGCAT CATGATTCTT ATCTGTCTCA ACATGGTCAC CATGATGGTG 
GAAACCGACG ACCAGAGCGA CTGTGTGACT AGCATTTTGT CCCGCATCAA CTTGGTGTTC 
ATTGTGCTGT TCACTGGGGA GTGTGTGCTG AAGCTCATCT CCCTCCGCCA TTACTATTTT 
ACTATAGGCT GGAATATCTT TGATTTTGTG GTGGTCATTC TCTCCATAGT AGGTATGTTT 
CTGGCTGAGC TGATAGAGAA ATATTTTGTG TCCCCTACCT TGTTCCGAGT GATCCGTCTT 
GCCAGGATTG GCCGAATCCT GCGTCTGATC AAAGGGGCTA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTGCT CTTCCTGGTC 
ATGTTCATCT ATGCCATCTT TGGAATGTCC AACTTCGCCT ATGTTAAGAG GGAGGTTGGA 
ATTGATGACA TGTTTAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT GTTCCAGATT 
ACCACCTCTG CTGGCTGGGA TGGATTGCTA GCACCTATTC TCAACAGTAA ACCACCCGAT 
TGTGACCCTA ATAAAGTTAA CCCTGGCAGC TCAGTTAAAG GAGACTGTGG GAACCCATCT 
GTTGGGATTT TCTTCTTTGT CAGTTACATC ATCATATCAT TTCTAGTCGT GGTGAACATG 
TATATAGCTG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGCGC AGAGCCCCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGACGCCACC 
CAGTTCATGG AATTTGAAAA GCTATCTCAG TTTGCAGCTG CTCTTGAACC ACCGCTCAAT 
TTGCCACAAC CGAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT AAGTGGTGAT 
CGAATCCACT GTCTTGACAT TTTATTTGCT TTTACGAAGC GGGTTCTGGG GGAGAGTGGA 
GAGATGGATG CCCTTCGAAT ACAGATGGAA GAGAGATTCA TGGCCTCCAA CCCATCGAAA 
GTCTCCTATC AGCCAATAAC TACGACTTTA AAGAGAAAAC AAGAGGAAGT GTCTGCTGTT 
ATTATTCAGC GTGCATACAG GCGCCACCTT TTAAAGCGAA CTGTAAAACA GGCATCATTT 
ACGTACAACA AAAACAAAAT CAAGGGTGGG GCTAATCTTC TTGTAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCAATTACA GAAAAAACTG ATCTGACCAT GTCCACAGCA 
GCTTGTCCAC CCTCCTATGA TCGGGTAACA AAACCAATTG TGGAAAAGCA CGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005202502.1
CDS	166..6195
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDEHGPKPNSDLEAGKNLPFIYGDVPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHTVEKNITKNYNGTFVNETRNEFDWKSYIQDSRYHYFLEGFSDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAVTASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKINSECKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGIAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCMSNHTTEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVVEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDMFDISEVNNHSDCLILIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDCVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_005202445.3
Organism	Bos taurus(cattle)
Definition	Bos taurus sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005202445.3

ORF Insert Sequence:

ATGGAACAAA CAGTGCTTGT GCCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCCCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CTAAGAATCC CAAACCAGAC 
AAGAAAGACG ATGATGAACA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC CGGAAAGAAC 
CTTCCTTTTA TTTATGGAGA CGTCCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GATCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCCGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG AGACCCATGG AACTGGCTCG ACTTCACTGT CATTACATTT 
GCATATGTGA CAGAGTTTGT GGACCTGGGC AATGTCTCCG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCGC TGAAAACAAT TTCAGTCATT CCAGGTCTGA AAACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAAAAGCT CTCAGATGTC ATGATCCTAA CTGTGTTCTG TTTGAGTGTG 
TTCGCCCTGA TTGGGCTGCA GCTCTTTATG GGCAACCTGC GGAATAAATG TGTGCAGTGG 
CCTCCCACCA ACGCTTCCCT GGAGGAGCAC ACTGTAGAGA AGAACATAAC CAAGAATTAC 
AATGGCACAT TTGTAAACGA AACGCGAAAT GAGTTTGACT GGAAGTCGTA TATTCAAGAT 
TCAAGATATC ATTATTTTCT GGAGGGTTTT TCAGATGCAC TACTGTGTGG CAATAGCTCA 
GATGCGGGCC AATGTCCAGA AGGATACATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CACTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAGCTG ACATTACGTG CTGCTGGAAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCCTTCTATC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA AGAACAGAAT CAGGCCACCT TAGAAGAGGC GGAACAGAAA 
GAGGCTGAAT TTCAGCAGAT GCTCGAACAA CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAGCTG TAACTGCCTC AGAGCATTCC AGAGAACCTA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCGT CTGAAGCCTC CAAGTTGAGT TCCAAGAGTG CAAAGGAAAG AAGAAATCGG 
AGAAAGAAAA GAAAACAGAA AGAGCAATCT GGTGGAGAAG AGAAAGATGA TGATGAATTC 
CAGAAATCTG AATCTGAAGA CAGCATCAGG AGGAAAGGGT TTCGCTTCTC CATAGAAGGG 
AATCGGTTGA CATACGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCTTAT TTTCTCCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT CAGAGGGCGA 
GCAAAGGATG TGGGATCAGA GAACGACTTT GCTGATGATG AGCACAGCAC CTTCGAGGAT 
AACGAGAGCC GCAGAGACTC CTTATTTGTG CCCCGACGGC ATGGAGAGCG ACGCAACAGT 
AACTTAAGTC AGACCAGTAG GTCGTCCCGA ATGCTGGCCG TGTTTCCAGC CAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCGGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT TCATGTTTCC 
ATGGACTTTC TAGAAGATCC ATCTCAAAGG CAAAGAGCAA TGAGCATAGC CAGCATTTTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCGCCATACT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCTTTTGTT GACCTGGCCA TCACCATCTG CATTGTCTTA 
AACACTCTCT TCATGGCCAT GGAGCACTAT CCCATGACAG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGATGG TTTTATTGTA 
ACGCTTAGCC TGGTAGAACT TGGCCTGGCC AACGTGGAAG GATTATCAGT TCTCCGTTCA 
TTCCGTTTGC TTCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACATTAAA TATGCTAATA 
AAGATCATCG GCAATTCAGT GGGGGCTCTG GGAAACCTAA CCTTGGTGTT AGCCATCATC 
GTCTTCATTT TTGCTGTGGT TGGCATGCAG CTCTTTGGTA AAAGTTACAA AGATTGTGTC 
TGCAAGATCA ACAGTGAGTG TAAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGGGT GCTGTGTGGA GAGTGGATAG AGACCATGTG GGACTGCATG 
GAGGTCGCCG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT CATTGGAAAC 
CTGGTGGTCC TGAACCTCTT TTTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCGGCCACTG ATGATGATAA TGAAATGAAC AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAATAG CTTATGTGAA GAGAAAAATA TATGAATTTA TTCAGCAGTC CTTTGTTAGA 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAG GAAAGACAAT 
TGTATGTCCA ATCATACAAC AGAGATTGGG AAAGACCTTG ACTATCTTAA AGACGTAAAT 
GGAACCACAA GTGGAATAGG AACCGGCAGC AGTGTTGAAA AGTACATTAT TGATGAAAGT 
GATTACATGT CCTTCATAAA CAATCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACAGAG GACTTCAGTA GTGAATCAGA TCTGGAAGAA 
AGCAAAGAGA AGCTTAATGA AAGCAGTAGT TCATCTGAAG GTAGCACTGT AGACATTGGA 
GCACCTGCCG AGGAACAGCC TGTGGTGGAA CCCGAGGAAA CCCTTGAACC TGAAGCCTGT 
TTCACTGAAG GCTGTGTGCA AAGATTCAAA TGTTGTCAAA TCAGTGTGGA AGAAGGGAGA 
GGAAAACAGT GGTGGAATCT GAGAAGAACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CACTGGCATT TGAGGATATA 
TATATTGACC AGCGAAAGAC AATCAAAACA ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT AGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGT GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCACGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTA CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTGT TAACACCACA 
ACTGGTGACA TGTTTGACAT CAGCGAAGTG AATAATCATT CAGATTGCCT AATACTAATA 
GAAAGAAATG AGACTGCCCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTTAAGGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AGGAAAGTCT ATACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAAAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCAAA AAAGCCACAA 
AAGCCTATAC CTCGACCAGG AAACAAATTC CAAGGAATGG TCTTTGACTT CGTCACCAGA 
CAAGTTTTTG ACATCAGCAT CATGATTCTT ATCTGTCTCA ACATGGTCAC CATGATGGTG 
GAAACCGACG ACCAGAGCGA CTGTGTGACT AGCATTTTGT CCCGCATCAA CTTGGTGTTC 
ATTGTGCTGT TCACTGGGGA GTGTGTGCTG AAGCTCATCT CCCTCCGCCA TTACTATTTT 
ACTATAGGCT GGAATATCTT TGATTTTGTG GTGGTCATTC TCTCCATAGT AGGTATGTTT 
CTGGCTGAGC TGATAGAGAA ATATTTTGTG TCCCCTACCT TGTTCCGAGT GATCCGTCTT 
GCCAGGATTG GCCGAATCCT GCGTCTGATC AAAGGGGCTA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTGCT CTTCCTGGTC 
ATGTTCATCT ATGCCATCTT TGGAATGTCC AACTTCGCCT ATGTTAAGAG GGAGGTTGGA 
ATTGATGACA TGTTTAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT GTTCCAGATT 
ACCACCTCTG CTGGCTGGGA TGGATTGCTA GCACCTATTC TCAACAGTAA ACCACCCGAT 
TGTGACCCTA ATAAAGTTAA CCCTGGCAGC TCAGTTAAAG GAGACTGTGG GAACCCATCT 
GTTGGGATTT TCTTCTTTGT CAGTTACATC ATCATATCAT TTCTAGTCGT GGTGAACATG 
TATATAGCTG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGCGC AGAGCCCCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGACGCCACC 
CAGTTCATGG AATTTGAAAA GCTATCTCAG TTTGCAGCTG CTCTTGAACC ACCGCTCAAT 
TTGCCACAAC CGAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT AAGTGGTGAT 
CGAATCCACT GTCTTGACAT TTTATTTGCT TTTACGAAGC GGGTTCTGGG GGAGAGTGGA 
GAGATGGATG CCCTTCGAAT ACAGATGGAA GAGAGATTCA TGGCCTCCAA CCCATCGAAA 
GTCTCCTATC AGCCAATAAC TACGACTTTA AAGAGAAAAC AAGAGGAAGT GTCTGCTGTT 
ATTATTCAGC GTGCATACAG GCGCCACCTT TTAAAGCGAA CTGTAAAACA GGCATCATTT 
ACGTACAACA AAAACAAAAT CAAGGGTGGG GCTAATCTTC TTGTAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCAATTACA GAAAAAACTG ATCTGACCAT GTCCACAGCA 
GCTTGTCCAC CCTCCTATGA TCGGGTAACA AAACCAATTG TGGAAAAGCA CGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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SCN1A cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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