Scn1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Scn1a
Entrez Gene ID	81574
Full Name	sodium voltage-gated channel alpha subunit 1
General protein information	Preferred Names sodium voltage-gated channel alpha subunit 1 Names sodium channel protein type 1 subunit alpha sodium channel protein type I subunit alpha sodium channel protein, brain I subunit alpha sodium channel, voltage-gated, type 1, alpha polypeptide sodium channel, voltage-gated, type I, alpha polypeptide sodium channel, voltage-gated, type I, alpha subunit voltage-gated sodium channel subunit alpha Nav1.1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q21
Summary	has voltage gated sodium ion channel activity; plays a role in generation of action potential [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017592057.1	XP_017447546.1	sodium channel protein type 1 subunit alpha isoform X4
NM_030875.1	NP_110502.1	sodium channel protein type 1 subunit alpha
XM_008761923.2	XP_008760145.1	sodium channel protein type 1 subunit alpha isoform X1
XM_017592055.1	XP_017447544.1	sodium channel protein type 1 subunit alpha isoform X2
XM_008761925.2	XP_008760147.1	sodium channel protein type 1 subunit alpha isoform X1
XM_008761924.1	XP_008760146.1	sodium channel protein type 1 subunit alpha isoform X1
XM_017592058.1	XP_017447547.1	sodium channel protein type 1 subunit alpha isoform X5
XM_017592056.1	XP_017447545.1	sodium channel protein type 1 subunit alpha isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	SCN1A	NP_001159435.1
Mus musculus (house mouse)	Scn1a	NP_061203.2
Gallus gallus (chicken)	SCN1A	XP_003641634.1
	SCN1A	XP_001101023.1
Bos taurus (cattle)	SCN1A	NP_001180147.1
Pan troglodytes (chimpanzee)	SCN1A	XP_003309330.1
Canis lupus familiaris (dog)	SCN1A	XP_005640320.1
Rattus norvegicus (Norway rat)	Scn1a	NP_110502.1

Gene Ontology
Bibliography

Related articles in PubMed

Selective spider toxins reveal a role for the Nav1.1 channel in mechanical pain.
Osteen JD, Herzig V, Gilchrist J, Emrick JJ, Zhang C, Wang X, Castro J, Garcia-Caraballo S, Grundy L, Rychkov GY, Weyer AD, Dekan Z, Undheim EA, Alewood P, Stucky CL, Brierley SM, Basbaum AI, Bosmans F, King GF, Julius D
Nature534(7608)494-9(2016 06)

Variants of Transient Receptor Potential Melastatin Member 4 in Childhood Atrioventricular Block.
Syam N, Chatel S, Ozhathil LC, Sottas V, Rougier JS, Baruteau A, Baron E, Amarouch MY, Daumy X, Probst V, Schott JJ, Abriel H
Journal of the American Heart Association5(5)(2016 May)

Cognitive Deficits Associated with Nav1.1 Alterations: Involvement of Neuronal Firing Dynamics and Oscillations.
Bender AC, Luikart BW, Lenck-Santini PP
PloS one11(3)e0151538(2016)

Age-dependent alterations of voltage-gated Na(+) channel isoforms in rat sinoatrial node.
Huang X, Du Y, Yang P, Lin S, Xi Y, Yang Z, Ma A
Mechanisms of ageing and development15280-90(2015 Dec)

MicroRNA-9 induces defective trafficking of Nav1.1 and Nav1.2 by targeting Nav?2 protein coding region in rat with chronic brain hypoperfusion.
Sun LH, Yan ML, Hu XL, Peng LW, Che H, Bao YN, Guo F, Liu T, Chen X, Zhang R, Ban T, Wang N, Liu HL, Hou X, Ai J
Molecular neurodegeneration1036(2015 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Nav1.1 in facilitates fast-firing properties in medial septum neurons. Nav1.1 deficits may disrupt information processing in Dravet syndrome via a dysregulation of theta wave brain rhythms.
Title: Cognitive Deficits Associated with Nav1.1 Alterations: Involvement of Neuronal Firing Dynamics and Oscillations.

the presences of Nav1.1, Nav1.6, Navbeta1 and Navbeta3 mRNA and their reduced levels in rat SAN during aging.
Title: Age-dependent alterations of voltage-gated Na(+) channel isoforms in rat sinoatrial node.

miR-9 suppressed the trafficking of Nav1.1/Nav1.2 from cytoplasm to cell membrane. miR-9 overexpression inhibited Navbeta2 expression by targeting its coding sequence domain.
Title: MicroRNA-9 induces defective trafficking of Nav1.1 and Nav1.2 by targeting Nav?2 protein coding region in rat with chronic brain hypoperfusion.

Scn1a mutant rats demonstrate a higher hyperthermia-induced seizure susceptibility than wild type rats.
Title: Inhalation of 10% carbon dioxide rapidly terminates Scn1a mutation-related hyperthermia-induced seizures.

We show that reduction of Nav1.1 expression in the medial septum and diagonal band of Broca leads to a dysregulation of hippocampal oscillations in association with a spatial memory deficit
Title: Focal Scn1a knockdown induces cognitive impairment without seizures.

The following Scn1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Scn1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa43709	XM_017592056.1 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa43710	XM_017592057.1 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa43711	XM_017592058.1 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
ORa13702	NM_030875.1 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa43707	XM_008761924.1 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa43707	XM_008761923.2 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa43707	XM_008761925.2 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
ORa43708	XM_017592055.1 Latest version!	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Scn1a ( XM_017592056.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447545.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa43709
Clone ID Related Accession (Same CDS sequence)	XM_017592056.1
Accession Version	XM_017592056.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 1 subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGCAG 
GCGGCGGCAG CAACAGCATC AGAACATTCC AGGGAGCCCA GTGCAGCGGG CAGGCTCTCA 
GATAGCTCTT CAGAAGCCTC CAAGTTGAGT TCAAAGAGCG CTAAAGAAAG GCGGAATCGG 
AGGAAAAAAA GAAAACAGAA AGAGCAGTCT GGAGGGGAAG AGAAAGATGA TGATGAATTC 
CACAAATCCG AGTCCGAAGA TAGCATCAGG AGGAAGGGCT TCCGCTTCTC CATAGAAGGG 
AACAGACTGA CCTATGAAAA GAGGTACTCT TCCCCACATC AGTCTCTGTT GAGCATTCGC 
GGCTCACTGT TTTCCCCGAG ACGCAATAGC AGAACAAGTC TTTTCAGCTT CAGAGGGCGA 
GCGAAGGACG TGGGGTCTGA GAATGATTTT GCGGACGATG AGCACAGCAC CTTCGAGGAC 
AATGAGAGCA GGAGAGACTC TCTGTTTGTT CCCCGAAGAC ATGGAGAGCG ACGCAACAGT 
AACCTCAGCC AAACCAGCAG ATCCTCCCGA ATGCTGGCGG GGCTTCCAGC AAACGGGAAG 
ATGCACAGCA CAGTGGATTG CAATGGTGTG GTTTCCTTGG TGGGCGGACC CTCGGTTCCG 
ACATCGCCAG TTGGACAGCT TCTGCCAGAG GGAACAACTA CTGAAACTGA GATGAGAAAG 
AGGAGGTCAA GTTCTTTCCA TGTTTCCATG GACTTTCTTG AGGATCCTTC CCAGAGGCAA 
AGGGCAATGA GCATTGCCAG CATCTTAACA AATACAGTAG AAGAACTAGA AGAATCCAGA 
CAGAAATGTC CACCCTGTTG GTATAAATTT TCCAACATAT TTTTAATTTG GGACTGTTCT 
CCATATTGGC TAAAAGTTAA ACATATTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCAATTA CTATCTGTAT TGTGTTAAAT ACCCTTTTCA TGGCCATGGA GCACTACCCC 
ATGACTGAAC ATTTCAATCA TGTACTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTC 
ACAGCAGAAA TGTTCCTGAA AATCATTGCC ATGGACCCTT ACTATTATTT CCAAGAGGGC 
TGGAATATCT TTGATGGTTT CATTGTGACA CTCAGCCTGG TAGAACTTGG CCTTGCCAAT 
GTGGAAGGGT TATCAGTTCT CCGTTCATTC CGACTGCTCC GAGTCTTCAA GTTGGCAAAG 
TCCTGGCCCA CACTGAACAT GCTCATTAAG ATCATCGGCA ACTCGGTGGG CGCACTGGGC 
AACCTGACCC TGGTGCTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTG 
TTCGGCAAAA GTTACAAAGA TTGTGTCTGC AAAATTGCCA CTGACTGCAA ACTCCCGCGC 
TGGCACATGA ACGACTTCTT CCACTCCTTC CTGATCGTGT TCCGAGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGCATGGAG GTCGCGGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGGAACCTT GTGGTCCTGA ACCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGACG ATGACAACGA AATGAACAAC 
CTTCAAATTG CTGTGGACAG GATGCACAAA GGAGTAGCTT ATGTAAAAAG AAAAATATAT 
GAGTTTATTC AACAGTCCTT TGTTAGGAAA CAGAAGATCC TAGATGAAAT TAAGCCACTT 
GATGATCTAA ACAACAGAAA AGACAATTGT ACATCTAACC ACACGACAGA GATTGGGAAA 
GATCTGGACT GTCTGAAAGA TGTGAATGGA ACCACGAGTG GCATAGGGAC CGGCAGCAGT 
GTGGAAAAGT ACATCATTGA TGAGAGTGAC TACATGTCAT TCATAAACAA CCCCAGCCTC 
ACCGTGACTG TGCCCATTGC TGTGGGAGAG TCTGACTTTG AAAACTTAAA CACAGAGGAC 
TTTAGCAGTG AATCAGATCT AGAAGAAAGC AAAGAGAAAC TCAACGAAAG CAGTAGTTCC 
TCAGAAGGAA GCACAGTAGA CATCGGGGCA CCTGCGGAGG AACAGCCTGT CATGGAACCA 
GAAGAAACCC TTGAGCCCGA AGCTTGCTTC ACTGAAGGCT GTGTGCAGAG ATTCAAGTGC 
TGTCAAATCA GTGTGGAAGA AGGAAGAGGA AAACAGTGGT GGAACCTTCG GAGAACGTGC 
TTCCGAATAG TTGAGCACAA CTGGTTTGAG ACCTTCATTG TGTTCATGAT TCTCCTCAGT 
AGTGGTGCCC TGGCCTTTGA GGATATATAC ATTGATCAGC GAAAGACGAT CAAGACCATG 
CTGGAGTATG CAGACAAGGT TTTCACTTAC ATTTTTATCC TGGAGATGCT CCTCAAATGG 
GTAGCCTACG GCTATCAAAC GTATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCTGA ACTTGGGGCC 
ATCAAGTCCC TAAGGACACT AAGAGCTCTG AGACCCCTAA GAGCCTTATC ACGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTGTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGCC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTGTTAA CACCACAACT GGTGACACAT TTGAGATCAC CGAAGTCAAT 
AATCATTCTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCCCGGTG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTTTCTT TGCTTCAAGT TGCGACGTTT 
AAGGGTTGGA TGGATATCAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTGCAGCCT 
AAGTATGAGG AAAGCCTGTA CATGTACCTG TACTTCGTCA TCTTCATCAT CTTCGGCTCG 
TTCTTCACTC TAAATCTATT CATCGGTGTC ATCATAGACA ACTTCAACCA GCAGAAGAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAGAAATTAG GATCAAAAAA GCCACAAAAG CCTATCCCTC GACCTGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTTGT AACCAGACAA GTGTTTGACA TCAGCATCAT GATCCTCATC 
TGTCTGAACA TGGTGACCAT GATGGTGGAA ACGGATGACC AGAGCGATTA TGTGACAAGC 
ATTTTGTCAC GTATCAACCT GGTGTTCATT GTGCTGTTCA CCGGCGAGTG CGTGCTGAAG 
CTCATCTCCC TCCGCCATTA TTATTTCACC ATTGGCTGGA ATATTTTCGA TTTTGTGGTG 
GTCATCCTCT CCATTGTAGG AATGTTTCTT GCGGAGCTGA TAGAGAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTCAT CCGCCTGGCC AGGATTGGAC GAATCCTACG CCTGATCAAA 
GGCGCCAAGG GGATCCGCAC TCTGCTCTTT GCTCTGATGA TGTCCCTTCC TGCGCTGTTC 
AACATCGGCC TCCTGCTTTT CCTGGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGAATT GATGACATGT TCAACTTTGA GACTTTTGGC 
AACAGCATGA TCTGCCTGTT CCAAATCACC ACCTCTGCGG GCTGGGACGG ACTGCTGGCC 
CCCATCCTCA ACAGCAAACC CCCTGACTGT GACCCTAATA AAGTTAACCC CGGAAGCTCG 
GTGAAGGGGG ACTGTGGGAA CCCATCCGTG GGGATTTTCT TTTTTGTCAG CTACATCATC 
ATATCCTTCC TGGTGGTGGT GAACATGTAC ATCGCTGTCA TCCTGGAGAA CTTCAGCGTC 
GCCACCGAAG AAAGTGCAGA GCCCCTGAGT GAGGACGACT TTGAGATGTT CTACGAGGTC 
TGGGAGAAGT TCGACCCTGA CGCCACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCTC TCGAACCCCC TCTCAACTTA CCACAACCGA ACAAACTTCA GCTCATTGCC 
ATGGACCTGC CCATGGTGAG TGGAGACCGC ATCCACTGCC TGGACATCTT ATTTGCTTTT 
ACAAAGCGGG TCCTGGGCGA GAGTGGAGAG ATGGACGCTC TTCGAATCCA GATGGAAGAG 
CGCTTCATGG CTTCCAACCC CTCCAAGGTC TCCTATCAGC CAATCACTAC AACGTTAAAA 
CGAAAGCAAG AGGAGGTGTC AGCCGTTATC ATTCAGCGCG CTTACAGGCG CCACCTTTTG 
AAGCGAACAG TAAAACAAGC TTCATTCACA TACAATAAGA ACAAACTCAA GGGCGGGGCT 
AATCTTCTTG TAAAAGAAGA CATGATCATT GACAGGATAA ATGAAAACTC TATTACAGAG 
AAAACTGACC TGACGATGTC CACTGCAGCT TGTCCGCCCT CCTACGACCG GGTGACAAAG 
CCAATCGTGG AGAAACACGA GCAGGAAGGA AAAGATGAGA AAGCCAAAGG GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447545.1
CDS	180..6176
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEKNVTTDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFHKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAGLPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCTSNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVMEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDTFEITEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKLKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017592056.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592056.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGCAG 
GCGGCGGCAG CAACAGCATC AGAACATTCC AGGGAGCCCA GTGCAGCGGG CAGGCTCTCA 
GATAGCTCTT CAGAAGCCTC CAAGTTGAGT TCAAAGAGCG CTAAAGAAAG GCGGAATCGG 
AGGAAAAAAA GAAAACAGAA AGAGCAGTCT GGAGGGGAAG AGAAAGATGA TGATGAATTC 
CACAAATCCG AGTCCGAAGA TAGCATCAGG AGGAAGGGCT TCCGCTTCTC CATAGAAGGG 
AACAGACTGA CCTATGAAAA GAGGTACTCT TCCCCACATC AGTCTCTGTT GAGCATTCGC 
GGCTCACTGT TTTCCCCGAG ACGCAATAGC AGAACAAGTC TTTTCAGCTT CAGAGGGCGA 
GCGAAGGACG TGGGGTCTGA GAATGATTTT GCGGACGATG AGCACAGCAC CTTCGAGGAC 
AATGAGAGCA GGAGAGACTC TCTGTTTGTT CCCCGAAGAC ATGGAGAGCG ACGCAACAGT 
AACCTCAGCC AAACCAGCAG ATCCTCCCGA ATGCTGGCGG GGCTTCCAGC AAACGGGAAG 
ATGCACAGCA CAGTGGATTG CAATGGTGTG GTTTCCTTGG TGGGCGGACC CTCGGTTCCG 
ACATCGCCAG TTGGACAGCT TCTGCCAGAG GGAACAACTA CTGAAACTGA GATGAGAAAG 
AGGAGGTCAA GTTCTTTCCA TGTTTCCATG GACTTTCTTG AGGATCCTTC CCAGAGGCAA 
AGGGCAATGA GCATTGCCAG CATCTTAACA AATACAGTAG AAGAACTAGA AGAATCCAGA 
CAGAAATGTC CACCCTGTTG GTATAAATTT TCCAACATAT TTTTAATTTG GGACTGTTCT 
CCATATTGGC TAAAAGTTAA ACATATTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCAATTA CTATCTGTAT TGTGTTAAAT ACCCTTTTCA TGGCCATGGA GCACTACCCC 
ATGACTGAAC ATTTCAATCA TGTACTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTC 
ACAGCAGAAA TGTTCCTGAA AATCATTGCC ATGGACCCTT ACTATTATTT CCAAGAGGGC 
TGGAATATCT TTGATGGTTT CATTGTGACA CTCAGCCTGG TAGAACTTGG CCTTGCCAAT 
GTGGAAGGGT TATCAGTTCT CCGTTCATTC CGACTGCTCC GAGTCTTCAA GTTGGCAAAG 
TCCTGGCCCA CACTGAACAT GCTCATTAAG ATCATCGGCA ACTCGGTGGG CGCACTGGGC 
AACCTGACCC TGGTGCTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTG 
TTCGGCAAAA GTTACAAAGA TTGTGTCTGC AAAATTGCCA CTGACTGCAA ACTCCCGCGC 
TGGCACATGA ACGACTTCTT CCACTCCTTC CTGATCGTGT TCCGAGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGCATGGAG GTCGCGGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGGAACCTT GTGGTCCTGA ACCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGACG ATGACAACGA AATGAACAAC 
CTTCAAATTG CTGTGGACAG GATGCACAAA GGAGTAGCTT ATGTAAAAAG AAAAATATAT 
GAGTTTATTC AACAGTCCTT TGTTAGGAAA CAGAAGATCC TAGATGAAAT TAAGCCACTT 
GATGATCTAA ACAACAGAAA AGACAATTGT ACATCTAACC ACACGACAGA GATTGGGAAA 
GATCTGGACT GTCTGAAAGA TGTGAATGGA ACCACGAGTG GCATAGGGAC CGGCAGCAGT 
GTGGAAAAGT ACATCATTGA TGAGAGTGAC TACATGTCAT TCATAAACAA CCCCAGCCTC 
ACCGTGACTG TGCCCATTGC TGTGGGAGAG TCTGACTTTG AAAACTTAAA CACAGAGGAC 
TTTAGCAGTG AATCAGATCT AGAAGAAAGC AAAGAGAAAC TCAACGAAAG CAGTAGTTCC 
TCAGAAGGAA GCACAGTAGA CATCGGGGCA CCTGCGGAGG AACAGCCTGT CATGGAACCA 
GAAGAAACCC TTGAGCCCGA AGCTTGCTTC ACTGAAGGCT GTGTGCAGAG ATTCAAGTGC 
TGTCAAATCA GTGTGGAAGA AGGAAGAGGA AAACAGTGGT GGAACCTTCG GAGAACGTGC 
TTCCGAATAG TTGAGCACAA CTGGTTTGAG ACCTTCATTG TGTTCATGAT TCTCCTCAGT 
AGTGGTGCCC TGGCCTTTGA GGATATATAC ATTGATCAGC GAAAGACGAT CAAGACCATG 
CTGGAGTATG CAGACAAGGT TTTCACTTAC ATTTTTATCC TGGAGATGCT CCTCAAATGG 
GTAGCCTACG GCTATCAAAC GTATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCTGA ACTTGGGGCC 
ATCAAGTCCC TAAGGACACT AAGAGCTCTG AGACCCCTAA GAGCCTTATC ACGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTGTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGCC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTGTTAA CACCACAACT GGTGACACAT TTGAGATCAC CGAAGTCAAT 
AATCATTCTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCCCGGTG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTTTCTT TGCTTCAAGT TGCGACGTTT 
AAGGGTTGGA TGGATATCAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTGCAGCCT 
AAGTATGAGG AAAGCCTGTA CATGTACCTG TACTTCGTCA TCTTCATCAT CTTCGGCTCG 
TTCTTCACTC TAAATCTATT CATCGGTGTC ATCATAGACA ACTTCAACCA GCAGAAGAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAGAAATTAG GATCAAAAAA GCCACAAAAG CCTATCCCTC GACCTGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTTGT AACCAGACAA GTGTTTGACA TCAGCATCAT GATCCTCATC 
TGTCTGAACA TGGTGACCAT GATGGTGGAA ACGGATGACC AGAGCGATTA TGTGACAAGC 
ATTTTGTCAC GTATCAACCT GGTGTTCATT GTGCTGTTCA CCGGCGAGTG CGTGCTGAAG 
CTCATCTCCC TCCGCCATTA TTATTTCACC ATTGGCTGGA ATATTTTCGA TTTTGTGGTG 
GTCATCCTCT CCATTGTAGG AATGTTTCTT GCGGAGCTGA TAGAGAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTCAT CCGCCTGGCC AGGATTGGAC GAATCCTACG CCTGATCAAA 
GGCGCCAAGG GGATCCGCAC TCTGCTCTTT GCTCTGATGA TGTCCCTTCC TGCGCTGTTC 
AACATCGGCC TCCTGCTTTT CCTGGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGAATT GATGACATGT TCAACTTTGA GACTTTTGGC 
AACAGCATGA TCTGCCTGTT CCAAATCACC ACCTCTGCGG GCTGGGACGG ACTGCTGGCC 
CCCATCCTCA ACAGCAAACC CCCTGACTGT GACCCTAATA AAGTTAACCC CGGAAGCTCG 
GTGAAGGGGG ACTGTGGGAA CCCATCCGTG GGGATTTTCT TTTTTGTCAG CTACATCATC 
ATATCCTTCC TGGTGGTGGT GAACATGTAC ATCGCTGTCA TCCTGGAGAA CTTCAGCGTC 
GCCACCGAAG AAAGTGCAGA GCCCCTGAGT GAGGACGACT TTGAGATGTT CTACGAGGTC 
TGGGAGAAGT TCGACCCTGA CGCCACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCTC TCGAACCCCC TCTCAACTTA CCACAACCGA ACAAACTTCA GCTCATTGCC 
ATGGACCTGC CCATGGTGAG TGGAGACCGC ATCCACTGCC TGGACATCTT ATTTGCTTTT 
ACAAAGCGGG TCCTGGGCGA GAGTGGAGAG ATGGACGCTC TTCGAATCCA GATGGAAGAG 
CGCTTCATGG CTTCCAACCC CTCCAAGGTC TCCTATCAGC CAATCACTAC AACGTTAAAA 
CGAAAGCAAG AGGAGGTGTC AGCCGTTATC ATTCAGCGCG CTTACAGGCG CCACCTTTTG 
AAGCGAACAG TAAAACAAGC TTCATTCACA TACAATAAGA ACAAACTCAA GGGCGGGGCT 
AATCTTCTTG TAAAAGAAGA CATGATCATT GACAGGATAA ATGAAAACTC TATTACAGAG 
AAAACTGACC TGACGATGTC CACTGCAGCT TGTCCGCCCT CCTACGACCG GGTGACAAAG 
CCAATCGTGG AGAAACACGA GCAGGAAGGA AAAGATGAGA AAGCCAAAGG GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn1a ( XM_017592057.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447546.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X7, mRNA.

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CloneID	ORa43710
Clone ID Related Accession (Same CDS sequence)	XM_017592057.1
Accession Version	XM_017592057.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 1 subunit alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGGCG 
GCGGCAGCAA CAGCATCAGA ACATTCCAGG GAGCCCAGTG CAGCGGGCAG GCTCTCAGAT 
AGCTCTTCAG AAGCCTCCAA GTTGAGTTCA AAGAGCGCTA AAGAAAGGCG GAATCGGAGG 
AAAAAAAGAA AACAGAAAGA GCAGTCTGGA GGGGAAGAGA AAGATGATGA TGAATTCCAC 
AAATCCGAGT CCGAAGATAG CATCAGGAGG AAGGGCTTCC GCTTCTCCAT AGAAGGGAAC 
AGACTGACCT ATGAAAAGAG GTACTCTTCC CCACATCAGT CTCTGTTGAG CATTCGCGGC 
TCACTGTTTT CCCCGAGACG CAATAGCAGA ACAAGTCTTT TCAGCTTCAG AGGGCGAGCG 
AAGGACGTGG GGTCTGAGAA TGATTTTGCG GACGATGAGC ACAGCACCTT CGAGGACAAT 
GAGAGCAGGA GAGACTCTCT GTTTGTTCCC CGAAGACATG GAGAGCGACG CAACAGTAAC 
CTCAGCCAAA CCAGCAGATC CTCCCGAATG CTGGCGGGGC TTCCAGCAAA CGGGAAGATG 
CACAGCACAG TGGATTGCAA TGGTGTGGTT TCCTTGGTGG GCGGACCCTC GGTTCCGACA 
TCGCCAGTTG GACAGCTTCT GCCAGAGGGA ACAACTACTG AAACTGAGAT GAGAAAGAGG 
AGGTCAAGTT CTTTCCATGT TTCCATGGAC TTTCTTGAGG ATCCTTCCCA GAGGCAAAGG 
GCAATGAGCA TTGCCAGCAT CTTAACAAAT ACAGTAGAAG AACTAGAAGA ATCCAGACAG 
AAATGTCCAC CCTGTTGGTA TAAATTTTCC AACATATTTT TAATTTGGGA CTGTTCTCCA 
TATTGGCTAA AAGTTAAACA TATTGTCAAC CTGGTTGTGA TGGACCCATT TGTTGACCTG 
GCAATTACTA TCTGTATTGT GTTAAATACC CTTTTCATGG CCATGGAGCA CTACCCCATG 
ACTGAACATT TCAATCATGT ACTTACAGTA GGAAACTTGG TTTTCACTGG GATCTTCACA 
GCAGAAATGT TCCTGAAAAT CATTGCCATG GACCCTTACT ATTATTTCCA AGAGGGCTGG 
AATATCTTTG ATGGTTTCAT TGTGACACTC AGCCTGGTAG AACTTGGCCT TGCCAATGTG 
GAAGGGTTAT CAGTTCTCCG TTCATTCCGA CTGCTCCGAG TCTTCAAGTT GGCAAAGTCC 
TGGCCCACAC TGAACATGCT CATTAAGATC ATCGGCAACT CGGTGGGCGC ACTGGGCAAC 
CTGACCCTGG TGCTGGCCAT CATCGTCTTC ATTTTTGCCG TGGTCGGCAT GCAGCTGTTC 
GGCAAAAGTT ACAAAGATTG TGTCTGCAAA ATTGCCACTG ACTGCAAACT CCCGCGCTGG 
CACATGAACG ACTTCTTCCA CTCCTTCCTG ATCGTGTTCC GAGTGCTGTG TGGGGAGTGG 
ATAGAGACCA TGTGGGACTG CATGGAGGTC GCGGGTCAAG CCATGTGCCT TACTGTCTTC 
ATGATGGTCA TGGTGATTGG GAACCTTGTG GTCCTGAACC TCTTTCTGGC CTTGCTTCTG 
AGCTCATTTA GTGCAGACAA CCTTGCAGCC ACTGACGATG ACAACGAAAT GAACAACCTT 
CAAATTGCTG TGGACAGGAT GCACAAAGGA GTAGCTTATG TAAAAAGAAA AATATATGAG 
TTTATTCAAC AGTCCTTTGT TAGGAAACAG AAGATCCTAG ATGAAATTAA GCCACTTGAT 
GATCTAAACA ACAGAAAAGA CAATTGTACA TCTAACCACA CGACAGAGAT TGGGAAAGAT 
CTGGACTGTC TGAAAGATGT GAATGGAACC ACGAGTGGCA TAGGGACCGG CAGCAGTGTG 
GAAAAGTACA TCATTGATGA GAGTGACTAC ATGTCATTCA TAAACAACCC CAGCCTCACC 
GTGACTGTGC CCATTGCTGT GGGAGAGTCT GACTTTGAAA ACTTAAACAC AGAGGACTTT 
AGCAGTGAAT CAGATCTAGA AGAAAGCAAA GAGAAACTCA ACGAAAGCAG TAGTTCCTCA 
GAAGGAAGCA CAGTAGACAT CGGGGCACCT GCGGAGGAAC AGCCTGTCAT GGAACCAGAA 
GAAACCCTTG AGCCCGAAGC TTGCTTCACT GAAGGCTGTG TGCAGAGATT CAAGTGCTGT 
CAAATCAGTG TGGAAGAAGG AAGAGGAAAA CAGTGGTGGA ACCTTCGGAG AACGTGCTTC 
CGAATAGTTG AGCACAACTG GTTTGAGACC TTCATTGTGT TCATGATTCT CCTCAGTAGT 
GGTGCCCTGG CCTTTGAGGA TATATACATT GATCAGCGAA AGACGATCAA GACCATGCTG 
GAGTATGCAG ACAAGGTTTT CACTTACATT TTTATCCTGG AGATGCTCCT CAAATGGGTA 
GCCTACGGCT ATCAAACGTA TTTCACCAAT GCCTGGTGTT GGCTGGACTT CTTAATTGTT 
GATGTTTCAT TGGTCAGTTT AACAGCAAAT GCCTTGGGTT ACTCTGAACT TGGGGCCATC 
AAGTCCCTAA GGACACTAAG AGCTCTGAGA CCCCTAAGAG CCTTATCACG ATTTGAAGGG 
ATGAGGGTGG TTGTGAATGC CCTGTTAGGA GCAATTCCAT CCATCATGAA TGTGCTTCTG 
GTTTGCCTTA TATTCTGGCT AATTTTCAGC ATCATGGGCG TAAATTTGTT TGCTGGCAAA 
TTCTACCACT GTGTTAACAC CACAACTGGT GACACATTTG AGATCACCGA AGTCAATAAT 
CATTCTGATT GCCTAAAACT AATAGAAAGA AATGAGACTG CCCGGTGGAA AAATGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTTTCTTTGC TTCAAGTTGC GACGTTTAAG 
GGTTGGATGG ATATCATGTA TGCAGCAGTT GATTCCAGAA ATGTGGAACT GCAGCCTAAG 
TATGAGGAAA GCCTGTACAT GTACCTGTAC TTCGTCATCT TCATCATCTT CGGCTCGTTC 
TTCACTCTAA ATCTATTCAT CGGTGTCATC ATAGACAACT TCAACCAGCA GAAGAAGAAG 
TTTGGAGGTC AAGACATCTT TATGACAGAA GAACAGAAGA AATACTATAA TGCAATGAAG 
AAATTAGGAT CAAAAAAGCC ACAAAAGCCT ATCCCTCGAC CTGGAAACAA ATTTCAAGGA 
ATGGTCTTTG ACTTTGTAAC CAGACAAGTG TTTGACATCA GCATCATGAT CCTCATCTGT 
CTGAACATGG TGACCATGAT GGTGGAAACG GATGACCAGA GCGATTATGT GACAAGCATT 
TTGTCACGTA TCAACCTGGT GTTCATTGTG CTGTTCACCG GCGAGTGCGT GCTGAAGCTC 
ATCTCCCTCC GCCATTATTA TTTCACCATT GGCTGGAATA TTTTCGATTT TGTGGTGGTC 
ATCCTCTCCA TTGTAGGAAT GTTTCTTGCG GAGCTGATAG AGAAGTATTT CGTGTCCCCT 
ACCCTGTTCC GAGTCATCCG CCTGGCCAGG ATTGGACGAA TCCTACGCCT GATCAAAGGC 
GCCAAGGGGA TCCGCACTCT GCTCTTTGCT CTGATGATGT CCCTTCCTGC GCTGTTCAAC 
ATCGGCCTCC TGCTTTTCCT GGTCATGTTC ATCTACGCCA TCTTTGGGAT GTCCAACTTT 
GCCTATGTTA AGAGGGAAGT TGGAATTGAT GACATGTTCA ACTTTGAGAC TTTTGGCAAC 
AGCATGATCT GCCTGTTCCA AATCACCACC TCTGCGGGCT GGGACGGACT GCTGGCCCCC 
ATCCTCAACA GCAAACCCCC TGACTGTGAC CCTAATAAAG TTAACCCCGG AAGCTCGGTG 
AAGGGGGACT GTGGGAACCC ATCCGTGGGG ATTTTCTTTT TTGTCAGCTA CATCATCATA 
TCCTTCCTGG TGGTGGTGAA CATGTACATC GCTGTCATCC TGGAGAACTT CAGCGTCGCC 
ACCGAAGAAA GTGCAGAGCC CCTGAGTGAG GACGACTTTG AGATGTTCTA CGAGGTCTGG 
GAGAAGTTCG ACCCTGACGC CACTCAGTTC ATGGAATTTG AAAAATTATC TCAGTTTGCA 
GCTGCTCTCG AACCCCCTCT CAACTTACCA CAACCGAACA AACTTCAGCT CATTGCCATG 
GACCTGCCCA TGGTGAGTGG AGACCGCATC CACTGCCTGG ACATCTTATT TGCTTTTACA 
AAGCGGGTCC TGGGCGAGAG TGGAGAGATG GACGCTCTTC GAATCCAGAT GGAAGAGCGC 
TTCATGGCTT CCAACCCCTC CAAGGTCTCC TATCAGCCAA TCACTACAAC GTTAAAACGA 
AAGCAAGAGG AGGTGTCAGC CGTTATCATT CAGCGCGCTT ACAGGCGCCA CCTTTTGAAG 
CGAACAGTAA AACAAGCTTC ATTCACATAC AATAAGAACA AACTCAAGGG CGGGGCTAAT 
CTTCTTGTAA AAGAAGACAT GATCATTGAC AGGATAAATG AAAACTCTAT TACAGAGAAA 
ACTGACCTGA CGATGTCCAC TGCAGCTTGT CCGCCCTCCT ACGACCGGGT GACAAAGCCA 
ATCGTGGAGA AACACGAGCA GGAAGGAAAA GATGAGAAAG CCAAAGGGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447546.1
CDS	180..6173
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEKNVTTDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQAAAATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFHKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAGLPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCTSNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVMEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDTFEITEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKLKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017592057.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592057.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGGCG 
GCGGCAGCAA CAGCATCAGA ACATTCCAGG GAGCCCAGTG CAGCGGGCAG GCTCTCAGAT 
AGCTCTTCAG AAGCCTCCAA GTTGAGTTCA AAGAGCGCTA AAGAAAGGCG GAATCGGAGG 
AAAAAAAGAA AACAGAAAGA GCAGTCTGGA GGGGAAGAGA AAGATGATGA TGAATTCCAC 
AAATCCGAGT CCGAAGATAG CATCAGGAGG AAGGGCTTCC GCTTCTCCAT AGAAGGGAAC 
AGACTGACCT ATGAAAAGAG GTACTCTTCC CCACATCAGT CTCTGTTGAG CATTCGCGGC 
TCACTGTTTT CCCCGAGACG CAATAGCAGA ACAAGTCTTT TCAGCTTCAG AGGGCGAGCG 
AAGGACGTGG GGTCTGAGAA TGATTTTGCG GACGATGAGC ACAGCACCTT CGAGGACAAT 
GAGAGCAGGA GAGACTCTCT GTTTGTTCCC CGAAGACATG GAGAGCGACG CAACAGTAAC 
CTCAGCCAAA CCAGCAGATC CTCCCGAATG CTGGCGGGGC TTCCAGCAAA CGGGAAGATG 
CACAGCACAG TGGATTGCAA TGGTGTGGTT TCCTTGGTGG GCGGACCCTC GGTTCCGACA 
TCGCCAGTTG GACAGCTTCT GCCAGAGGGA ACAACTACTG AAACTGAGAT GAGAAAGAGG 
AGGTCAAGTT CTTTCCATGT TTCCATGGAC TTTCTTGAGG ATCCTTCCCA GAGGCAAAGG 
GCAATGAGCA TTGCCAGCAT CTTAACAAAT ACAGTAGAAG AACTAGAAGA ATCCAGACAG 
AAATGTCCAC CCTGTTGGTA TAAATTTTCC AACATATTTT TAATTTGGGA CTGTTCTCCA 
TATTGGCTAA AAGTTAAACA TATTGTCAAC CTGGTTGTGA TGGACCCATT TGTTGACCTG 
GCAATTACTA TCTGTATTGT GTTAAATACC CTTTTCATGG CCATGGAGCA CTACCCCATG 
ACTGAACATT TCAATCATGT ACTTACAGTA GGAAACTTGG TTTTCACTGG GATCTTCACA 
GCAGAAATGT TCCTGAAAAT CATTGCCATG GACCCTTACT ATTATTTCCA AGAGGGCTGG 
AATATCTTTG ATGGTTTCAT TGTGACACTC AGCCTGGTAG AACTTGGCCT TGCCAATGTG 
GAAGGGTTAT CAGTTCTCCG TTCATTCCGA CTGCTCCGAG TCTTCAAGTT GGCAAAGTCC 
TGGCCCACAC TGAACATGCT CATTAAGATC ATCGGCAACT CGGTGGGCGC ACTGGGCAAC 
CTGACCCTGG TGCTGGCCAT CATCGTCTTC ATTTTTGCCG TGGTCGGCAT GCAGCTGTTC 
GGCAAAAGTT ACAAAGATTG TGTCTGCAAA ATTGCCACTG ACTGCAAACT CCCGCGCTGG 
CACATGAACG ACTTCTTCCA CTCCTTCCTG ATCGTGTTCC GAGTGCTGTG TGGGGAGTGG 
ATAGAGACCA TGTGGGACTG CATGGAGGTC GCGGGTCAAG CCATGTGCCT TACTGTCTTC 
ATGATGGTCA TGGTGATTGG GAACCTTGTG GTCCTGAACC TCTTTCTGGC CTTGCTTCTG 
AGCTCATTTA GTGCAGACAA CCTTGCAGCC ACTGACGATG ACAACGAAAT GAACAACCTT 
CAAATTGCTG TGGACAGGAT GCACAAAGGA GTAGCTTATG TAAAAAGAAA AATATATGAG 
TTTATTCAAC AGTCCTTTGT TAGGAAACAG AAGATCCTAG ATGAAATTAA GCCACTTGAT 
GATCTAAACA ACAGAAAAGA CAATTGTACA TCTAACCACA CGACAGAGAT TGGGAAAGAT 
CTGGACTGTC TGAAAGATGT GAATGGAACC ACGAGTGGCA TAGGGACCGG CAGCAGTGTG 
GAAAAGTACA TCATTGATGA GAGTGACTAC ATGTCATTCA TAAACAACCC CAGCCTCACC 
GTGACTGTGC CCATTGCTGT GGGAGAGTCT GACTTTGAAA ACTTAAACAC AGAGGACTTT 
AGCAGTGAAT CAGATCTAGA AGAAAGCAAA GAGAAACTCA ACGAAAGCAG TAGTTCCTCA 
GAAGGAAGCA CAGTAGACAT CGGGGCACCT GCGGAGGAAC AGCCTGTCAT GGAACCAGAA 
GAAACCCTTG AGCCCGAAGC TTGCTTCACT GAAGGCTGTG TGCAGAGATT CAAGTGCTGT 
CAAATCAGTG TGGAAGAAGG AAGAGGAAAA CAGTGGTGGA ACCTTCGGAG AACGTGCTTC 
CGAATAGTTG AGCACAACTG GTTTGAGACC TTCATTGTGT TCATGATTCT CCTCAGTAGT 
GGTGCCCTGG CCTTTGAGGA TATATACATT GATCAGCGAA AGACGATCAA GACCATGCTG 
GAGTATGCAG ACAAGGTTTT CACTTACATT TTTATCCTGG AGATGCTCCT CAAATGGGTA 
GCCTACGGCT ATCAAACGTA TTTCACCAAT GCCTGGTGTT GGCTGGACTT CTTAATTGTT 
GATGTTTCAT TGGTCAGTTT AACAGCAAAT GCCTTGGGTT ACTCTGAACT TGGGGCCATC 
AAGTCCCTAA GGACACTAAG AGCTCTGAGA CCCCTAAGAG CCTTATCACG ATTTGAAGGG 
ATGAGGGTGG TTGTGAATGC CCTGTTAGGA GCAATTCCAT CCATCATGAA TGTGCTTCTG 
GTTTGCCTTA TATTCTGGCT AATTTTCAGC ATCATGGGCG TAAATTTGTT TGCTGGCAAA 
TTCTACCACT GTGTTAACAC CACAACTGGT GACACATTTG AGATCACCGA AGTCAATAAT 
CATTCTGATT GCCTAAAACT AATAGAAAGA AATGAGACTG CCCGGTGGAA AAATGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTTTCTTTGC TTCAAGTTGC GACGTTTAAG 
GGTTGGATGG ATATCATGTA TGCAGCAGTT GATTCCAGAA ATGTGGAACT GCAGCCTAAG 
TATGAGGAAA GCCTGTACAT GTACCTGTAC TTCGTCATCT TCATCATCTT CGGCTCGTTC 
TTCACTCTAA ATCTATTCAT CGGTGTCATC ATAGACAACT TCAACCAGCA GAAGAAGAAG 
TTTGGAGGTC AAGACATCTT TATGACAGAA GAACAGAAGA AATACTATAA TGCAATGAAG 
AAATTAGGAT CAAAAAAGCC ACAAAAGCCT ATCCCTCGAC CTGGAAACAA ATTTCAAGGA 
ATGGTCTTTG ACTTTGTAAC CAGACAAGTG TTTGACATCA GCATCATGAT CCTCATCTGT 
CTGAACATGG TGACCATGAT GGTGGAAACG GATGACCAGA GCGATTATGT GACAAGCATT 
TTGTCACGTA TCAACCTGGT GTTCATTGTG CTGTTCACCG GCGAGTGCGT GCTGAAGCTC 
ATCTCCCTCC GCCATTATTA TTTCACCATT GGCTGGAATA TTTTCGATTT TGTGGTGGTC 
ATCCTCTCCA TTGTAGGAAT GTTTCTTGCG GAGCTGATAG AGAAGTATTT CGTGTCCCCT 
ACCCTGTTCC GAGTCATCCG CCTGGCCAGG ATTGGACGAA TCCTACGCCT GATCAAAGGC 
GCCAAGGGGA TCCGCACTCT GCTCTTTGCT CTGATGATGT CCCTTCCTGC GCTGTTCAAC 
ATCGGCCTCC TGCTTTTCCT GGTCATGTTC ATCTACGCCA TCTTTGGGAT GTCCAACTTT 
GCCTATGTTA AGAGGGAAGT TGGAATTGAT GACATGTTCA ACTTTGAGAC TTTTGGCAAC 
AGCATGATCT GCCTGTTCCA AATCACCACC TCTGCGGGCT GGGACGGACT GCTGGCCCCC 
ATCCTCAACA GCAAACCCCC TGACTGTGAC CCTAATAAAG TTAACCCCGG AAGCTCGGTG 
AAGGGGGACT GTGGGAACCC ATCCGTGGGG ATTTTCTTTT TTGTCAGCTA CATCATCATA 
TCCTTCCTGG TGGTGGTGAA CATGTACATC GCTGTCATCC TGGAGAACTT CAGCGTCGCC 
ACCGAAGAAA GTGCAGAGCC CCTGAGTGAG GACGACTTTG AGATGTTCTA CGAGGTCTGG 
GAGAAGTTCG ACCCTGACGC CACTCAGTTC ATGGAATTTG AAAAATTATC TCAGTTTGCA 
GCTGCTCTCG AACCCCCTCT CAACTTACCA CAACCGAACA AACTTCAGCT CATTGCCATG 
GACCTGCCCA TGGTGAGTGG AGACCGCATC CACTGCCTGG ACATCTTATT TGCTTTTACA 
AAGCGGGTCC TGGGCGAGAG TGGAGAGATG GACGCTCTTC GAATCCAGAT GGAAGAGCGC 
TTCATGGCTT CCAACCCCTC CAAGGTCTCC TATCAGCCAA TCACTACAAC GTTAAAACGA 
AAGCAAGAGG AGGTGTCAGC CGTTATCATT CAGCGCGCTT ACAGGCGCCA CCTTTTGAAG 
CGAACAGTAA AACAAGCTTC ATTCACATAC AATAAGAACA AACTCAAGGG CGGGGCTAAT 
CTTCTTGTAA AAGAAGACAT GATCATTGAC AGGATAAATG AAAACTCTAT TACAGAGAAA 
ACTGACCTGA CGATGTCCAC TGCAGCTTGT CCGCCCTCCT ACGACCGGGT GACAAAGCCA 
ATCGTGGAGA AACACGAGCA GGAAGGAAAA GATGAGAAAG CCAAAGGGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn1a ( XM_017592058.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447547.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X8, mRNA.

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CloneID	ORa43711
Clone ID Related Accession (Same CDS sequence)	XM_017592058.1
Accession Version	XM_017592058.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 1 subunit alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGCAG 
GCGGCGGCAG CAACAGCATC AGAACATTCC AGGGAGCCCA GTGCAGCGGG CAGGCTCTCA 
GATAGCTCTT CAGAAGCCTC CAAGTTGAGT TCAAAGAGCG CTAAAGAAAG GCGGAATCGG 
AGGAAAAAAA GAAAACAGAA AGAGCAGTCT GGAGGGGAAG AGAAAGATGA TGATGAATTC 
CACAAATCCG AGTCCGAAGA TAGCATCAGG AGGAAGGGCT TCCGCTTCTC CATAGAAGGG 
AACAGACTGA CCTATGAAAA GAGGTACTCT TCCCCACATC AGTCTCTGTT GAGCATTCGC 
GGCTCACTGT TTTCCCCGAG ACGCAATAGC AGAACAAGTC TTTTCAGCTT CAGAGGGCGA 
GCGAAGGACG TGGGGTCTGA GAATGATTTT GCGGACGATG AGCACAGCAC CTTCGAGGAC 
AATGAGAGCA GGAGAGACTC TCTGTTTGTT CCCCGAAGAC ATGGAGAGCG ACGCAACAGT 
AACCTCAGCC AAACCAGCAG ATCCTCCCGA ATGCTGGCGG GGCTTCCAGC AAACGGGAAG 
ATGCACAGCA CAGTGGATTG CAATGGTGTG GTTTCCTTGG TGGGCGGACC CTCGGTTCCG 
ACATCGCCAG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CTACTGAAAC TGAGATGAGA AAGAGGAGGT CAAGTTCTTT CCATGTTTCC 
ATGGACTTTC TTGAGGATCC TTCCCAGAGG CAAAGGGCAA TGAGCATTGC CAGCATCTTA 
ACAAATACAG TAGAAGAACT AGAAGAATCC AGACAGAAAT GTCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTTTTAAT TTGGGACTGT TCTCCATATT GGCTAAAAGT TAAACATATT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCAA TTACTATCTG TATTGTGTTA 
AATACCCTTT TCATGGCCAT GGAGCACTAC CCCATGACTG AACATTTCAA TCATGTACTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTCACAGCAG AAATGTTCCT GAAAATCATT 
GCCATGGACC CTTACTATTA TTTCCAAGAG GGCTGGAATA TCTTTGATGG TTTCATTGTG 
ACACTCAGCC TGGTAGAACT TGGCCTTGCC AATGTGGAAG GGTTATCAGT TCTCCGTTCA 
TTCCGACTGC TCCGAGTCTT CAAGTTGGCA AAGTCCTGGC CCACACTGAA CATGCTCATT 
AAGATCATCG GCAACTCGGT GGGCGCACTG GGCAACCTGA CCCTGGTGCT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTGTTCGGCA AAAGTTACAA AGATTGTGTC 
TGCAAAATTG CCACTGACTG CAAACTCCCG CGCTGGCACA TGAACGACTT CTTCCACTCC 
TTCCTGATCG TGTTCCGAGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGCATG 
GAGGTCGCGG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGGAAC 
CTTGTGGTCC TGAACCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ACGATGACAA CGAAATGAAC AACCTTCAAA TTGCTGTGGA CAGGATGCAC 
AAAGGAGTAG CTTATGTAAA AAGAAAAATA TATGAGTTTA TTCAACAGTC CTTTGTTAGG 
AAACAGAAGA TCCTAGATGA AATTAAGCCA CTTGATGATC TAAACAACAG AAAAGACAAT 
TGTACATCTA ACCACACGAC AGAGATTGGG AAAGATCTGG ACTGTCTGAA AGATGTGAAT 
GGAACCACGA GTGGCATAGG GACCGGCAGC AGTGTGGAAA AGTACATCAT TGATGAGAGT 
GACTACATGT CATTCATAAA CAACCCCAGC CTCACCGTGA CTGTGCCCAT TGCTGTGGGA 
GAGTCTGACT TTGAAAACTT AAACACAGAG GACTTTAGCA GTGAATCAGA TCTAGAAGAA 
AGCAAAGAGA AACTCAACGA AAGCAGTAGT TCCTCAGAAG GAAGCACAGT AGACATCGGG 
GCACCTGCGG AGGAACAGCC TGTCATGGAA CCAGAAGAAA CCCTTGAGCC CGAAGCTTGC 
TTCACTGAAG GCTGTGTGCA GAGATTCAAG TGCTGTCAAA TCAGTGTGGA AGAAGGAAGA 
GGAAAACAGT GGTGGAACCT TCGGAGAACG TGCTTCCGAA TAGTTGAGCA CAACTGGTTT 
GAGACCTTCA TTGTGTTCAT GATTCTCCTC AGTAGTGGTG CCCTGGCCTT TGAGGATATA 
TACATTGATC AGCGAAAGAC GATCAAGACC ATGCTGGAGT ATGCAGACAA GGTTTTCACT 
TACATTTTTA TCCTGGAGAT GCTCCTCAAA TGGGTAGCCT ACGGCTATCA AACGTATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC TGAACTTGGG GCCATCAAGT CCCTAAGGAC ACTAAGAGCT 
CTGAGACCCC TAAGAGCCTT ATCACGATTT GAAGGGATGA GGGCGACGTT TAAGGGTTGG 
ATGGATATCA TGTATGCAGC AGTTGATTCC AGAAATGTGG AACTGCAGCC TAAGTATGAG 
GAAAGCCTGT ACATGTACCT GTACTTCGTC ATCTTCATCA TCTTCGGCTC GTTCTTCACT 
CTAAATCTAT TCATCGGTGT CATCATAGAC AACTTCAACC AGCAGAAGAA GAAGTTTGGA 
GGTCAAGACA TCTTTATGAC AGAAGAACAG AAGAAATACT ATAATGCAAT GAAGAAATTA 
GGATCAAAAA AGCCACAAAA GCCTATCCCT CGACCTGGAA ACAAATTTCA AGGAATGGTC 
TTTGACTTTG TAACCAGACA AGTGTTTGAC ATCAGCATCA TGATCCTCAT CTGTCTGAAC 
ATGGTGACCA TGATGGTGGA AACGGATGAC CAGAGCGATT ATGTGACAAG CATTTTGTCA 
CGTATCAACC TGGTGTTCAT TGTGCTGTTC ACCGGCGAGT GCGTGCTGAA GCTCATCTCC 
CTCCGCCATT ATTATTTCAC CATTGGCTGG AATATTTTCG ATTTTGTGGT GGTCATCCTC 
TCCATTGTAG GAATGTTTCT TGCGGAGCTG ATAGAGAAGT ATTTCGTGTC CCCTACCCTG 
TTCCGAGTCA TCCGCCTGGC CAGGATTGGA CGAATCCTAC GCCTGATCAA AGGCGCCAAG 
GGGATCCGCA CTCTGCTCTT TGCTCTGATG ATGTCCCTTC CTGCGCTGTT CAACATCGGC 
CTCCTGCTTT TCCTGGTCAT GTTCATCTAC GCCATCTTTG GGATGTCCAA CTTTGCCTAT 
GTTAAGAGGG AAGTTGGAAT TGATGACATG TTCAACTTTG AGACTTTTGG CAACAGCATG 
ATCTGCCTGT TCCAAATCAC CACCTCTGCG GGCTGGGACG GACTGCTGGC CCCCATCCTC 
AACAGCAAAC CCCCTGACTG TGACCCTAAT AAAGTTAACC CCGGAAGCTC GGTGAAGGGG 
GACTGTGGGA ACCCATCCGT GGGGATTTTC TTTTTTGTCA GCTACATCAT CATATCCTTC 
CTGGTGGTGG TGAACATGTA CATCGCTGTC ATCCTGGAGA ACTTCAGCGT CGCCACCGAA 
GAAAGTGCAG AGCCCCTGAG TGAGGACGAC TTTGAGATGT TCTACGAGGT CTGGGAGAAG 
TTCGACCCTG ACGCCACTCA GTTCATGGAA TTTGAAAAAT TATCTCAGTT TGCAGCTGCT 
CTCGAACCCC CTCTCAACTT ACCACAACCG AACAAACTTC AGCTCATTGC CATGGACCTG 
CCCATGGTGA GTGGAGACCG CATCCACTGC CTGGACATCT TATTTGCTTT TACAAAGCGG 
GTCCTGGGCG AGAGTGGAGA GATGGACGCT CTTCGAATCC AGATGGAAGA GCGCTTCATG 
GCTTCCAACC CCTCCAAGGT CTCCTATCAG CCAATCACTA CAACGTTAAA ACGAAAGCAA 
GAGGAGGTGT CAGCCGTTAT CATTCAGCGC GCTTACAGGC GCCACCTTTT GAAGCGAACA 
GTAAAACAAG CTTCATTCAC ATACAATAAG AACAAACTCA AGGGCGGGGC TAATCTTCTT 
GTAAAAGAAG ACATGATCAT TGACAGGATA AATGAAAACT CTATTACAGA GAAAACTGAC 
CTGACGATGT CCACTGCAGC TTGTCCGCCC TCCTACGACC GGGTGACAAA GCCAATCGTG 
GAGAAACACG AGCAGGAAGG AAAAGATGAG AAAGCCAAAG GGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447547.1
CDS	180..5927
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEKNVTTDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFHKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAGLPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCTSNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVMEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKLKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017592058.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592058.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGCAG 
GCGGCGGCAG CAACAGCATC AGAACATTCC AGGGAGCCCA GTGCAGCGGG CAGGCTCTCA 
GATAGCTCTT CAGAAGCCTC CAAGTTGAGT TCAAAGAGCG CTAAAGAAAG GCGGAATCGG 
AGGAAAAAAA GAAAACAGAA AGAGCAGTCT GGAGGGGAAG AGAAAGATGA TGATGAATTC 
CACAAATCCG AGTCCGAAGA TAGCATCAGG AGGAAGGGCT TCCGCTTCTC CATAGAAGGG 
AACAGACTGA CCTATGAAAA GAGGTACTCT TCCCCACATC AGTCTCTGTT GAGCATTCGC 
GGCTCACTGT TTTCCCCGAG ACGCAATAGC AGAACAAGTC TTTTCAGCTT CAGAGGGCGA 
GCGAAGGACG TGGGGTCTGA GAATGATTTT GCGGACGATG AGCACAGCAC CTTCGAGGAC 
AATGAGAGCA GGAGAGACTC TCTGTTTGTT CCCCGAAGAC ATGGAGAGCG ACGCAACAGT 
AACCTCAGCC AAACCAGCAG ATCCTCCCGA ATGCTGGCGG GGCTTCCAGC AAACGGGAAG 
ATGCACAGCA CAGTGGATTG CAATGGTGTG GTTTCCTTGG TGGGCGGACC CTCGGTTCCG 
ACATCGCCAG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CTACTGAAAC TGAGATGAGA AAGAGGAGGT CAAGTTCTTT CCATGTTTCC 
ATGGACTTTC TTGAGGATCC TTCCCAGAGG CAAAGGGCAA TGAGCATTGC CAGCATCTTA 
ACAAATACAG TAGAAGAACT AGAAGAATCC AGACAGAAAT GTCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTTTTAAT TTGGGACTGT TCTCCATATT GGCTAAAAGT TAAACATATT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCAA TTACTATCTG TATTGTGTTA 
AATACCCTTT TCATGGCCAT GGAGCACTAC CCCATGACTG AACATTTCAA TCATGTACTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTCACAGCAG AAATGTTCCT GAAAATCATT 
GCCATGGACC CTTACTATTA TTTCCAAGAG GGCTGGAATA TCTTTGATGG TTTCATTGTG 
ACACTCAGCC TGGTAGAACT TGGCCTTGCC AATGTGGAAG GGTTATCAGT TCTCCGTTCA 
TTCCGACTGC TCCGAGTCTT CAAGTTGGCA AAGTCCTGGC CCACACTGAA CATGCTCATT 
AAGATCATCG GCAACTCGGT GGGCGCACTG GGCAACCTGA CCCTGGTGCT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTGTTCGGCA AAAGTTACAA AGATTGTGTC 
TGCAAAATTG CCACTGACTG CAAACTCCCG CGCTGGCACA TGAACGACTT CTTCCACTCC 
TTCCTGATCG TGTTCCGAGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGCATG 
GAGGTCGCGG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGGAAC 
CTTGTGGTCC TGAACCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ACGATGACAA CGAAATGAAC AACCTTCAAA TTGCTGTGGA CAGGATGCAC 
AAAGGAGTAG CTTATGTAAA AAGAAAAATA TATGAGTTTA TTCAACAGTC CTTTGTTAGG 
AAACAGAAGA TCCTAGATGA AATTAAGCCA CTTGATGATC TAAACAACAG AAAAGACAAT 
TGTACATCTA ACCACACGAC AGAGATTGGG AAAGATCTGG ACTGTCTGAA AGATGTGAAT 
GGAACCACGA GTGGCATAGG GACCGGCAGC AGTGTGGAAA AGTACATCAT TGATGAGAGT 
GACTACATGT CATTCATAAA CAACCCCAGC CTCACCGTGA CTGTGCCCAT TGCTGTGGGA 
GAGTCTGACT TTGAAAACTT AAACACAGAG GACTTTAGCA GTGAATCAGA TCTAGAAGAA 
AGCAAAGAGA AACTCAACGA AAGCAGTAGT TCCTCAGAAG GAAGCACAGT AGACATCGGG 
GCACCTGCGG AGGAACAGCC TGTCATGGAA CCAGAAGAAA CCCTTGAGCC CGAAGCTTGC 
TTCACTGAAG GCTGTGTGCA GAGATTCAAG TGCTGTCAAA TCAGTGTGGA AGAAGGAAGA 
GGAAAACAGT GGTGGAACCT TCGGAGAACG TGCTTCCGAA TAGTTGAGCA CAACTGGTTT 
GAGACCTTCA TTGTGTTCAT GATTCTCCTC AGTAGTGGTG CCCTGGCCTT TGAGGATATA 
TACATTGATC AGCGAAAGAC GATCAAGACC ATGCTGGAGT ATGCAGACAA GGTTTTCACT 
TACATTTTTA TCCTGGAGAT GCTCCTCAAA TGGGTAGCCT ACGGCTATCA AACGTATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC TGAACTTGGG GCCATCAAGT CCCTAAGGAC ACTAAGAGCT 
CTGAGACCCC TAAGAGCCTT ATCACGATTT GAAGGGATGA GGGCGACGTT TAAGGGTTGG 
ATGGATATCA TGTATGCAGC AGTTGATTCC AGAAATGTGG AACTGCAGCC TAAGTATGAG 
GAAAGCCTGT ACATGTACCT GTACTTCGTC ATCTTCATCA TCTTCGGCTC GTTCTTCACT 
CTAAATCTAT TCATCGGTGT CATCATAGAC AACTTCAACC AGCAGAAGAA GAAGTTTGGA 
GGTCAAGACA TCTTTATGAC AGAAGAACAG AAGAAATACT ATAATGCAAT GAAGAAATTA 
GGATCAAAAA AGCCACAAAA GCCTATCCCT CGACCTGGAA ACAAATTTCA AGGAATGGTC 
TTTGACTTTG TAACCAGACA AGTGTTTGAC ATCAGCATCA TGATCCTCAT CTGTCTGAAC 
ATGGTGACCA TGATGGTGGA AACGGATGAC CAGAGCGATT ATGTGACAAG CATTTTGTCA 
CGTATCAACC TGGTGTTCAT TGTGCTGTTC ACCGGCGAGT GCGTGCTGAA GCTCATCTCC 
CTCCGCCATT ATTATTTCAC CATTGGCTGG AATATTTTCG ATTTTGTGGT GGTCATCCTC 
TCCATTGTAG GAATGTTTCT TGCGGAGCTG ATAGAGAAGT ATTTCGTGTC CCCTACCCTG 
TTCCGAGTCA TCCGCCTGGC CAGGATTGGA CGAATCCTAC GCCTGATCAA AGGCGCCAAG 
GGGATCCGCA CTCTGCTCTT TGCTCTGATG ATGTCCCTTC CTGCGCTGTT CAACATCGGC 
CTCCTGCTTT TCCTGGTCAT GTTCATCTAC GCCATCTTTG GGATGTCCAA CTTTGCCTAT 
GTTAAGAGGG AAGTTGGAAT TGATGACATG TTCAACTTTG AGACTTTTGG CAACAGCATG 
ATCTGCCTGT TCCAAATCAC CACCTCTGCG GGCTGGGACG GACTGCTGGC CCCCATCCTC 
AACAGCAAAC CCCCTGACTG TGACCCTAAT AAAGTTAACC CCGGAAGCTC GGTGAAGGGG 
GACTGTGGGA ACCCATCCGT GGGGATTTTC TTTTTTGTCA GCTACATCAT CATATCCTTC 
CTGGTGGTGG TGAACATGTA CATCGCTGTC ATCCTGGAGA ACTTCAGCGT CGCCACCGAA 
GAAAGTGCAG AGCCCCTGAG TGAGGACGAC TTTGAGATGT TCTACGAGGT CTGGGAGAAG 
TTCGACCCTG ACGCCACTCA GTTCATGGAA TTTGAAAAAT TATCTCAGTT TGCAGCTGCT 
CTCGAACCCC CTCTCAACTT ACCACAACCG AACAAACTTC AGCTCATTGC CATGGACCTG 
CCCATGGTGA GTGGAGACCG CATCCACTGC CTGGACATCT TATTTGCTTT TACAAAGCGG 
GTCCTGGGCG AGAGTGGAGA GATGGACGCT CTTCGAATCC AGATGGAAGA GCGCTTCATG 
GCTTCCAACC CCTCCAAGGT CTCCTATCAG CCAATCACTA CAACGTTAAA ACGAAAGCAA 
GAGGAGGTGT CAGCCGTTAT CATTCAGCGC GCTTACAGGC GCCACCTTTT GAAGCGAACA 
GTAAAACAAG CTTCATTCAC ATACAATAAG AACAAACTCA AGGGCGGGGC TAATCTTCTT 
GTAAAAGAAG ACATGATCAT TGACAGGATA AATGAAAACT CTATTACAGA GAAAACTGAC 
CTGACGATGT CCACTGCAGC TTGTCCGCCC TCCTACGACC GGGTGACAAA GCCAATCGTG 
GAGAAACACG AGCAGGAAGG AAAAGATGAG AAAGCCAAAG GGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn1a ( NM_030875.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_110502.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), mRNA.

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CloneID	ORa13702
Clone ID Related Accession (Same CDS sequence)	NM_030875.1
Accession Version	NM_030875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 1 subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from M22253.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M22253.1, X03638.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGCAG 
GCGGCGGCAG CAACAGCATC AGAACATTCC AGGGAGCCCA GTGCAGCGGG CAGGCTCTCA 
GATAGCTCTT CAGAAGCCTC CAAGTTGAGT TCAAAGAGCG CTAAAGAAAG GCGGAATCGG 
AGGAAAAAAA GAAAACAGAA AGAGCAGTCT GGAGGGGAAG AGAAAGATGA TGATGAATTC 
CACAAATCCG AGTCCGAAGA TAGCATCAGG AGGAAGGGCT TCCGCTTCTC CATAGAAGGG 
AACAGACTGA CCTATGAAAA GAGGTACTCT TCCCCACATC AGTCTCTGTT GAGCATTCGC 
GGCTCACTGT TTTCCCCGAG ACGCAATAGC AGAACAAGTC TTTTCAGCTT CAGAGGGCGA 
GCGAAGGACG TGGGGTCTGA GAATGATTTT GCGGACGATG AGCACAGCAC CTTCGAGGAC 
AATGAGAGCA GGAGAGACTC TCTGTTTGTT CCCCGAAGAC ATGGAGAGCG ACGCAACAGT 
AACCTCAGCC AAACCAGCAG ATCCTCCCGA ATGCTGGCGG GGCTTCCAGC AAACGGGAAG 
ATGCACAGCA CAGTGGATTG CAATGGTGTG GTTTCCTTGG TGGGCGGACC CTCGGTTCCG 
ACATCGCCAG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CTACTGAAAC TGAGATGAGA AAGAGGAGGT CAAGTTCTTT CCATGTTTCC 
ATGGACTTTC TTGAGGATCC TTCCCAGAGG CAAAGGGCAA TGAGCATTGC CAGCATCTTA 
ACAAATACAG TAGAAGAACT AGAAGAATCC AGACAGAAAT GTCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTTTTAAT TTGGGACTGT TCTCCATATT GGCTAAAAGT TAAACATATT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCAA TTACTATCTG TATTGTGTTA 
AATACCCTTT TCATGGCCAT GGAGCACTAC CCCATGACTG AACATTTCAA TCATGTACTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTCACAGCAG AAATGTTCCT GAAAATCATT 
GCCATGGACC CTTACTATTA TTTCCAAGAG GGCTGGAATA TCTTTGATGG TTTCATTGTG 
ACACTCAGCC TGGTAGAACT TGGCCTTGCC AATGTGGAAG GGTTATCAGT TCTCCGTTCA 
TTCCGACTGC TCCGAGTCTT CAAGTTGGCA AAGTCCTGGC CCACACTGAA CATGCTCATT 
AAGATCATCG GCAACTCGGT GGGCGCACTG GGCAACCTGA CCCTGGTGCT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTGTTCGGCA AAAGTTACAA AGATTGTGTC 
TGCAAAATTG CCACTGACTG CAAACTCCCG CGCTGGCACA TGAACGACTT CTTCCACTCC 
TTCCTGATCG TGTTCCGAGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGCATG 
GAGGTCGCGG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTAGGAAC 
CTTGTGGTCC TGAACCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ACGATGACAA CGAAATGAAC AACCTTCAAA TTGCTGTGGA CAGGATGCAC 
AAAGGAGTAG CTTATGTAAA AAGAAAAATA TATGAGTTTA TTCAACAGTC CTTTGTTAGG 
AAACAGAAGA TCCTAGATGA AATTAAGCCA CTTGATGATC TAAACAACAG AAAAGACAAT 
TGTACATCTA ACCACACGAC AGAGATTGGG AAAGATCTGG ACTGTCTGAA AGATGTGAAT 
GGAACCACGA GTGGCATAGG GACCGGCAGC AGTGTGGAAA AGTACATCAT TGATGAGAGT 
GACTACATGT CATTCATAAA CAACCCCAGC CTCACCGTGA CTGTGCCCAT TGCTGTGGGA 
GAGTCTGACT TTGAAAACTT AAACACAGAG GACTTTAGCA GTGAATCAGA TCTAGAAGAA 
AGCAAAGAGA AACTCAACGA AAGCAGTAGT TCCTCAGAAG GAAGCACAGT AGACATCGGG 
GCGCCTGCGG AGGAACAGCC TGTCATGGAA CCAGAAGAAA CCCTTGAGCC CGAAGCTTGC 
TTCACTGAAG GCTGTGTGCA GAGATTCAAG TGCTGTCAAA TCAGTGTGGA AGAAGGAAGA 
GGAAAACAGT GGTGGAACCT TCGGAGAACG TGCTTCCGAA TAGTTGAGCA CAACTGGTTT 
GAGACCTTCA TTGTGTTCAT GATTCTCCTC AGTAGTGGTG CCCTGGCCTT TGAGGATATA 
TACATTGATC AGCGAAAGAC AATCAAGACC ATGCTGGAGT ATGCAGACAA GGTTTTCACT 
TACATTTTTA TCCTGGAGAT GCTCCTCAAA TGGGTAGCCT ACGGCTATCA AACGTATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC TGAACTTGGG GCCATCAAGT CCCTAAGGAC ACTAAGAGCT 
CTGAGACCCC TAAGAGCCTT ATCACGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTG 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GCCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTGT TAACACCACA 
ACTGGTGACA CATTTGAGAT CACCGAAGTC AATAATCATT CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCCCG GTGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTTT CTTTGCTTCA AGTTGCGACG TTTAAGGGTT GGATGGATAT CATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTGCAG CCTAAGTATG AGGAAAGCCT GTACATGTAC 
CTGTACTTCG TCATCTTCAT CATCTTCGGC TCGTTCTTCA CTCTAAATCT ATTCATCGGT 
GTCATCATAG ACAACTTCAA CCAGCAGAAG AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAGAAAT TAGGATCAAA AAAGCCACAA 
AAGCCTATCC CTCGACCTGG AAACAAATTT CAAGGAATGG TCTTTGACTT TGTAACCAGA 
CAAGTGTTTG ACATCAGCAT CATGATCCTC ATCTGTCTGA ACATGGTGAC CATGATGGTG 
GAAACGGATG ACCAGAGCGA TTATGTGACA AGCATTTTGT CACGTATCAA CCTGGTGTTC 
ATTGTGCTGT TCACCGGCGA GTGCGTGCTG AAGCTCATCT CCCTCCGCCA TTATTATTTC 
ACCATTGGCT GGAATATTTT CGATTTTGTG GTGGTCATCC TCTCCATTGT AGGAATGTTT 
CTTGCGGAGC TGATAGAGAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT CATCCGCCTG 
GCCAGGATTG GACGAATCCT ACGCCTGATC AAAGGCGCCA AGGGGATCCG CACTCTGCTC 
TTTGCTCTGA TGATGTCCCT TCCTGCGCTG TTCAACATCG GCCTCCTGCT TTTCCTGGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGA 
ATTGATGACA TGTTCAACTT TGAGACTTTT GGCAACAGCA TGATCTGCCT GTTCCAAATC 
ACCACCTCTG CGGGCTGGGA CGGACTGCTG GCCCCCATCC TCAACAGCAA ACCCCCTGAC 
TGTGACCCTA ATAAAGTTAA CCCCGGAAGC TCGGTGAAGG GGGACTGTGG GAACCCATCC 
GTGGGGATTT TCTTTTTTGT CAGCTACATC ATCATATCCT TCCTGGTGGT GGTGAACATG 
TACATCGCTG TCATCCTGGA GAACTTCAGC GTTGCCACCG AAGAAAGTGC AGAGCCCCTG 
AGTGAGGACG ACTTTGAGAT GTTCTACGAG GTCTGGGAGA AGTTCGACCC TGACGCCACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CTCTCGAACC CCCTCTCAAC 
TTACCACAAC CGAACAAACT TCAGCTCATT GCCATGGACC TGCCCATGGT GAGTGGAGAC 
CGCATCCACT GCCTGGACAT CTTGTTTGCT TTTACAAAGC GGGTCTTGGG CGAGAGTGGA 
GAGATGGACG CTCTTCGAAT CCAGATGGAA GAAAGGTTCA TGGCTTCCAA CCCCTCCAAG 
GTCTCCTATC AGCCAATCAC TACAACGTTA AAACGAAAGC AAGAGGAGGT GTCAGCCGTT 
ATCATTCAGC GCGCTTACAG GCGCCACCTT TTGAAGCGAA CAGTAAAACA AGCTTCATTC 
ACATACAATA AGAACAAACT CAAGGGCGGG GCTAATCTTC TTGTAAAAGA AGACATGATC 
ATTGACAGGA TAAATGAAAA CTCTATTACA GAGAAAACTG ACCTGACGAT GTCCACTGCA 
GCTTGTCCGC CCTCCTACGA CCGGGTGACA AAGCCAATCG TGGAGAAACA CGAGCAGGAA 
GGAAAAGATG AGAAAGCCAA AGGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_110502.1
CDS	252..6281
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEKNVTTDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFHKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAGLPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIRNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCTSNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVMEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDTFEITEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKLKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_030875.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030875.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGCAG 
GCGGCGGCAG CAACAGCATC AGAACATTCC AGGGAGCCCA GTGCAGCGGG CAGGCTCTCA 
GATAGCTCTT CAGAAGCCTC CAAGTTGAGT TCAAAGAGCG CTAAAGAAAG GCGGAATCGG 
AGGAAAAAAA GAAAACAGAA AGAGCAGTCT GGAGGGGAAG AGAAAGATGA TGATGAATTC 
CACAAATCCG AGTCCGAAGA TAGCATCAGG AGGAAGGGCT TCCGCTTCTC CATAGAAGGG 
AACAGACTGA CCTATGAAAA GAGGTACTCT TCCCCACATC AGTCTCTGTT GAGCATTCGC 
GGCTCACTGT TTTCCCCGAG ACGCAATAGC AGAACAAGTC TTTTCAGCTT CAGAGGGCGA 
GCGAAGGACG TGGGGTCTGA GAATGATTTT GCGGACGATG AGCACAGCAC CTTCGAGGAC 
AATGAGAGCA GGAGAGACTC TCTGTTTGTT CCCCGAAGAC ATGGAGAGCG ACGCAACAGT 
AACCTCAGCC AAACCAGCAG ATCCTCCCGA ATGCTGGCGG GGCTTCCAGC AAACGGGAAG 
ATGCACAGCA CAGTGGATTG CAATGGTGTG GTTTCCTTGG TGGGCGGACC CTCGGTTCCG 
ACATCGCCAG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CTACTGAAAC TGAGATGAGA AAGAGGAGGT CAAGTTCTTT CCATGTTTCC 
ATGGACTTTC TTGAGGATCC TTCCCAGAGG CAAAGGGCAA TGAGCATTGC CAGCATCTTA 
ACAAATACAG TAGAAGAACT AGAAGAATCC AGACAGAAAT GTCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTTTTAAT TTGGGACTGT TCTCCATATT GGCTAAAAGT TAAACATATT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCAA TTACTATCTG TATTGTGTTA 
AATACCCTTT TCATGGCCAT GGAGCACTAC CCCATGACTG AACATTTCAA TCATGTACTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTCACAGCAG AAATGTTCCT GAAAATCATT 
GCCATGGACC CTTACTATTA TTTCCAAGAG GGCTGGAATA TCTTTGATGG TTTCATTGTG 
ACACTCAGCC TGGTAGAACT TGGCCTTGCC AATGTGGAAG GGTTATCAGT TCTCCGTTCA 
TTCCGACTGC TCCGAGTCTT CAAGTTGGCA AAGTCCTGGC CCACACTGAA CATGCTCATT 
AAGATCATCG GCAACTCGGT GGGCGCACTG GGCAACCTGA CCCTGGTGCT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTGTTCGGCA AAAGTTACAA AGATTGTGTC 
TGCAAAATTG CCACTGACTG CAAACTCCCG CGCTGGCACA TGAACGACTT CTTCCACTCC 
TTCCTGATCG TGTTCCGAGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGCATG 
GAGGTCGCGG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTAGGAAC 
CTTGTGGTCC TGAACCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ACGATGACAA CGAAATGAAC AACCTTCAAA TTGCTGTGGA CAGGATGCAC 
AAAGGAGTAG CTTATGTAAA AAGAAAAATA TATGAGTTTA TTCAACAGTC CTTTGTTAGG 
AAACAGAAGA TCCTAGATGA AATTAAGCCA CTTGATGATC TAAACAACAG AAAAGACAAT 
TGTACATCTA ACCACACGAC AGAGATTGGG AAAGATCTGG ACTGTCTGAA AGATGTGAAT 
GGAACCACGA GTGGCATAGG GACCGGCAGC AGTGTGGAAA AGTACATCAT TGATGAGAGT 
GACTACATGT CATTCATAAA CAACCCCAGC CTCACCGTGA CTGTGCCCAT TGCTGTGGGA 
GAGTCTGACT TTGAAAACTT AAACACAGAG GACTTTAGCA GTGAATCAGA TCTAGAAGAA 
AGCAAAGAGA AACTCAACGA AAGCAGTAGT TCCTCAGAAG GAAGCACAGT AGACATCGGG 
GCGCCTGCGG AGGAACAGCC TGTCATGGAA CCAGAAGAAA CCCTTGAGCC CGAAGCTTGC 
TTCACTGAAG GCTGTGTGCA GAGATTCAAG TGCTGTCAAA TCAGTGTGGA AGAAGGAAGA 
GGAAAACAGT GGTGGAACCT TCGGAGAACG TGCTTCCGAA TAGTTGAGCA CAACTGGTTT 
GAGACCTTCA TTGTGTTCAT GATTCTCCTC AGTAGTGGTG CCCTGGCCTT TGAGGATATA 
TACATTGATC AGCGAAAGAC AATCAAGACC ATGCTGGAGT ATGCAGACAA GGTTTTCACT 
TACATTTTTA TCCTGGAGAT GCTCCTCAAA TGGGTAGCCT ACGGCTATCA AACGTATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC TGAACTTGGG GCCATCAAGT CCCTAAGGAC ACTAAGAGCT 
CTGAGACCCC TAAGAGCCTT ATCACGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTG 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GCCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTGT TAACACCACA 
ACTGGTGACA CATTTGAGAT CACCGAAGTC AATAATCATT CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCCCG GTGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTTT CTTTGCTTCA AGTTGCGACG TTTAAGGGTT GGATGGATAT CATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTGCAG CCTAAGTATG AGGAAAGCCT GTACATGTAC 
CTGTACTTCG TCATCTTCAT CATCTTCGGC TCGTTCTTCA CTCTAAATCT ATTCATCGGT 
GTCATCATAG ACAACTTCAA CCAGCAGAAG AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAGAAAT TAGGATCAAA AAAGCCACAA 
AAGCCTATCC CTCGACCTGG AAACAAATTT CAAGGAATGG TCTTTGACTT TGTAACCAGA 
CAAGTGTTTG ACATCAGCAT CATGATCCTC ATCTGTCTGA ACATGGTGAC CATGATGGTG 
GAAACGGATG ACCAGAGCGA TTATGTGACA AGCATTTTGT CACGTATCAA CCTGGTGTTC 
ATTGTGCTGT TCACCGGCGA GTGCGTGCTG AAGCTCATCT CCCTCCGCCA TTATTATTTC 
ACCATTGGCT GGAATATTTT CGATTTTGTG GTGGTCATCC TCTCCATTGT AGGAATGTTT 
CTTGCGGAGC TGATAGAGAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT CATCCGCCTG 
GCCAGGATTG GACGAATCCT ACGCCTGATC AAAGGCGCCA AGGGGATCCG CACTCTGCTC 
TTTGCTCTGA TGATGTCCCT TCCTGCGCTG TTCAACATCG GCCTCCTGCT TTTCCTGGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGA 
ATTGATGACA TGTTCAACTT TGAGACTTTT GGCAACAGCA TGATCTGCCT GTTCCAAATC 
ACCACCTCTG CGGGCTGGGA CGGACTGCTG GCCCCCATCC TCAACAGCAA ACCCCCTGAC 
TGTGACCCTA ATAAAGTTAA CCCCGGAAGC TCGGTGAAGG GGGACTGTGG GAACCCATCC 
GTGGGGATTT TCTTTTTTGT CAGCTACATC ATCATATCCT TCCTGGTGGT GGTGAACATG 
TACATCGCTG TCATCCTGGA GAACTTCAGC GTTGCCACCG AAGAAAGTGC AGAGCCCCTG 
AGTGAGGACG ACTTTGAGAT GTTCTACGAG GTCTGGGAGA AGTTCGACCC TGACGCCACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CTCTCGAACC CCCTCTCAAC 
TTACCACAAC CGAACAAACT TCAGCTCATT GCCATGGACC TGCCCATGGT GAGTGGAGAC 
CGCATCCACT GCCTGGACAT CTTGTTTGCT TTTACAAAGC GGGTCTTGGG CGAGAGTGGA 
GAGATGGACG CTCTTCGAAT CCAGATGGAA GAAAGGTTCA TGGCTTCCAA CCCCTCCAAG 
GTCTCCTATC AGCCAATCAC TACAACGTTA AAACGAAAGC AAGAGGAGGT GTCAGCCGTT 
ATCATTCAGC GCGCTTACAG GCGCCACCTT TTGAAGCGAA CAGTAAAACA AGCTTCATTC 
ACATACAATA AGAACAAACT CAAGGGCGGG GCTAATCTTC TTGTAAAAGA AGACATGATC 
ATTGACAGGA TAAATGAAAA CTCTATTACA GAGAAAACTG ACCTGACGAT GTCCACTGCA 
GCTTGTCCGC CCTCCTACGA CCGGGTGACA AAGCCAATCG TGGAGAAACA CGAGCAGGAA 
GGAAAAGATG AGAAAGCCAA AGGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn1a ( XM_008761923.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760145.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X2, mRNA.

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CloneID	ORa43707
Clone ID Related Accession (Same CDS sequence)	XM_008761923.2 , XM_008761925.2 , XM_008761924.1
Accession Version	XM_008761923.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 1 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761923.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGCAG 
GCGGCGGCAG CAACAGCATC AGAACATTCC AGGGAGCCCA GTGCAGCGGG CAGGCTCTCA 
GATAGCTCTT CAGAAGCCTC CAAGTTGAGT TCAAAGAGCG CTAAAGAAAG GCGGAATCGG 
AGGAAAAAAA GAAAACAGAA AGAGCAGTCT GGAGGGGAAG AGAAAGATGA TGATGAATTC 
CACAAATCCG AGTCCGAAGA TAGCATCAGG AGGAAGGGCT TCCGCTTCTC CATAGAAGGG 
AACAGACTGA CCTATGAAAA GAGGTACTCT TCCCCACATC AGTCTCTGTT GAGCATTCGC 
GGCTCACTGT TTTCCCCGAG ACGCAATAGC AGAACAAGTC TTTTCAGCTT CAGAGGGCGA 
GCGAAGGACG TGGGGTCTGA GAATGATTTT GCGGACGATG AGCACAGCAC CTTCGAGGAC 
AATGAGAGCA GGAGAGACTC TCTGTTTGTT CCCCGAAGAC ATGGAGAGCG ACGCAACAGT 
AACCTCAGCC AAACCAGCAG ATCCTCCCGA ATGCTGGCGG GGCTTCCAGC AAACGGGAAG 
ATGCACAGCA CAGTGGATTG CAATGGTGTG GTTTCCTTGG TGGGCGGACC CTCGGTTCCG 
ACATCGCCAG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CTACTGAAAC TGAGATGAGA AAGAGGAGGT CAAGTTCTTT CCATGTTTCC 
ATGGACTTTC TTGAGGATCC TTCCCAGAGG CAAAGGGCAA TGAGCATTGC CAGCATCTTA 
ACAAATACAG TAGAAGAACT AGAAGAATCC AGACAGAAAT GTCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTTTTAAT TTGGGACTGT TCTCCATATT GGCTAAAAGT TAAACATATT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCAA TTACTATCTG TATTGTGTTA 
AATACCCTTT TCATGGCCAT GGAGCACTAC CCCATGACTG AACATTTCAA TCATGTACTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTCACAGCAG AAATGTTCCT GAAAATCATT 
GCCATGGACC CTTACTATTA TTTCCAAGAG GGCTGGAATA TCTTTGATGG TTTCATTGTG 
ACACTCAGCC TGGTAGAACT TGGCCTTGCC AATGTGGAAG GGTTATCAGT TCTCCGTTCA 
TTCCGACTGC TCCGAGTCTT CAAGTTGGCA AAGTCCTGGC CCACACTGAA CATGCTCATT 
AAGATCATCG GCAACTCGGT GGGCGCACTG GGCAACCTGA CCCTGGTGCT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTGTTCGGCA AAAGTTACAA AGATTGTGTC 
TGCAAAATTG CCACTGACTG CAAACTCCCG CGCTGGCACA TGAACGACTT CTTCCACTCC 
TTCCTGATCG TGTTCCGAGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGCATG 
GAGGTCGCGG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGGAAC 
CTTGTGGTCC TGAACCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ACGATGACAA CGAAATGAAC AACCTTCAAA TTGCTGTGGA CAGGATGCAC 
AAAGGAGTAG CTTATGTAAA AAGAAAAATA TATGAGTTTA TTCAACAGTC CTTTGTTAGG 
AAACAGAAGA TCCTAGATGA AATTAAGCCA CTTGATGATC TAAACAACAG AAAAGACAAT 
TGTACATCTA ACCACACGAC AGAGATTGGG AAAGATCTGG ACTGTCTGAA AGATGTGAAT 
GGAACCACGA GTGGCATAGG GACCGGCAGC AGTGTGGAAA AGTACATCAT TGATGAGAGT 
GACTACATGT CATTCATAAA CAACCCCAGC CTCACCGTGA CTGTGCCCAT TGCTGTGGGA 
GAGTCTGACT TTGAAAACTT AAACACAGAG GACTTTAGCA GTGAATCAGA TCTAGAAGAA 
AGCAAAGAGA AACTCAACGA AAGCAGTAGT TCCTCAGAAG GAAGCACAGT AGACATCGGG 
GCACCTGCGG AGGAACAGCC TGTCATGGAA CCAGAAGAAA CCCTTGAGCC CGAAGCTTGC 
TTCACTGAAG GCTGTGTGCA GAGATTCAAG TGCTGTCAAA TCAGTGTGGA AGAAGGAAGA 
GGAAAACAGT GGTGGAACCT TCGGAGAACG TGCTTCCGAA TAGTTGAGCA CAACTGGTTT 
GAGACCTTCA TTGTGTTCAT GATTCTCCTC AGTAGTGGTG CCCTGGCCTT TGAGGATATA 
TACATTGATC AGCGAAAGAC GATCAAGACC ATGCTGGAGT ATGCAGACAA GGTTTTCACT 
TACATTTTTA TCCTGGAGAT GCTCCTCAAA TGGGTAGCCT ACGGCTATCA AACGTATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC TGAACTTGGG GCCATCAAGT CCCTAAGGAC ACTAAGAGCT 
CTGAGACCCC TAAGAGCCTT ATCACGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTG 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GCCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTGT TAACACCACA 
ACTGGTGACA CATTTGAGAT CACCGAAGTC AATAATCATT CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCCCG GTGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTTT CTTTGCTTCA AGTTGCGACG TTTAAGGGTT GGATGGATAT CATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTGCAG CCTAAGTATG AGGAAAGCCT GTACATGTAC 
CTGTACTTCG TCATCTTCAT CATCTTCGGC TCGTTCTTCA CTCTAAATCT ATTCATCGGT 
GTCATCATAG ACAACTTCAA CCAGCAGAAG AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAGAAAT TAGGATCAAA AAAGCCACAA 
AAGCCTATCC CTCGACCTGG AAACAAATTT CAAGGAATGG TCTTTGACTT TGTAACCAGA 
CAAGTGTTTG ACATCAGCAT CATGATCCTC ATCTGTCTGA ACATGGTGAC CATGATGGTG 
GAAACGGATG ACCAGAGCGA TTATGTGACA AGCATTTTGT CACGTATCAA CCTGGTGTTC 
ATTGTGCTGT TCACCGGCGA GTGCGTGCTG AAGCTCATCT CCCTCCGCCA TTATTATTTC 
ACCATTGGCT GGAATATTTT CGATTTTGTG GTGGTCATCC TCTCCATTGT AGGAATGTTT 
CTTGCGGAGC TGATAGAGAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT CATCCGCCTG 
GCCAGGATTG GACGAATCCT ACGCCTGATC AAAGGCGCCA AGGGGATCCG CACTCTGCTC 
TTTGCTCTGA TGATGTCCCT TCCTGCGCTG TTCAACATCG GCCTCCTGCT TTTCCTGGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGA 
ATTGATGACA TGTTCAACTT TGAGACTTTT GGCAACAGCA TGATCTGCCT GTTCCAAATC 
ACCACCTCTG CGGGCTGGGA CGGACTGCTG GCCCCCATCC TCAACAGCAA ACCCCCTGAC 
TGTGACCCTA ATAAAGTTAA CCCCGGAAGC TCGGTGAAGG GGGACTGTGG GAACCCATCC 
GTGGGGATTT TCTTTTTTGT CAGCTACATC ATCATATCCT TCCTGGTGGT GGTGAACATG 
TACATCGCTG TCATCCTGGA GAACTTCAGC GTCGCCACCG AAGAAAGTGC AGAGCCCCTG 
AGTGAGGACG ACTTTGAGAT GTTCTACGAG GTCTGGGAGA AGTTCGACCC TGACGCCACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CTCTCGAACC CCCTCTCAAC 
TTACCACAAC CGAACAAACT TCAGCTCATT GCCATGGACC TGCCCATGGT GAGTGGAGAC 
CGCATCCACT GCCTGGACAT CTTATTTGCT TTTACAAAGC GGGTCCTGGG CGAGAGTGGA 
GAGATGGACG CTCTTCGAAT CCAGATGGAA GAGCGCTTCA TGGCTTCCAA CCCCTCCAAG 
GTCTCCTATC AGCCAATCAC TACAACGTTA AAACGAAAGC AAGAGGAGGT GTCAGCCGTT 
ATCATTCAGC GCGCTTACAG GCGCCACCTT TTGAAGCGAA CAGTAAAACA AGCTTCATTC 
ACATACAATA AGAACAAACT CAAGGGCGGG GCTAATCTTC TTGTAAAAGA AGACATGATC 
ATTGACAGGA TAAATGAAAA CTCTATTACA GAGAAAACTG ACCTGACGAT GTCCACTGCA 
GCTTGTCCGC CCTCCTACGA CCGGGTGACA AAGCCAATCG TGGAGAAACA CGAGCAGGAA 
GGAAAAGATG AGAAAGCCAA AGGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760145.1
CDS	170..6199
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEKNVTTDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFHKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAGLPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCTSNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVMEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDTFEITEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKLKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_008761923.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761923.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGCAG 
GCGGCGGCAG CAACAGCATC AGAACATTCC AGGGAGCCCA GTGCAGCGGG CAGGCTCTCA 
GATAGCTCTT CAGAAGCCTC CAAGTTGAGT TCAAAGAGCG CTAAAGAAAG GCGGAATCGG 
AGGAAAAAAA GAAAACAGAA AGAGCAGTCT GGAGGGGAAG AGAAAGATGA TGATGAATTC 
CACAAATCCG AGTCCGAAGA TAGCATCAGG AGGAAGGGCT TCCGCTTCTC CATAGAAGGG 
AACAGACTGA CCTATGAAAA GAGGTACTCT TCCCCACATC AGTCTCTGTT GAGCATTCGC 
GGCTCACTGT TTTCCCCGAG ACGCAATAGC AGAACAAGTC TTTTCAGCTT CAGAGGGCGA 
GCGAAGGACG TGGGGTCTGA GAATGATTTT GCGGACGATG AGCACAGCAC CTTCGAGGAC 
AATGAGAGCA GGAGAGACTC TCTGTTTGTT CCCCGAAGAC ATGGAGAGCG ACGCAACAGT 
AACCTCAGCC AAACCAGCAG ATCCTCCCGA ATGCTGGCGG GGCTTCCAGC AAACGGGAAG 
ATGCACAGCA CAGTGGATTG CAATGGTGTG GTTTCCTTGG TGGGCGGACC CTCGGTTCCG 
ACATCGCCAG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CTACTGAAAC TGAGATGAGA AAGAGGAGGT CAAGTTCTTT CCATGTTTCC 
ATGGACTTTC TTGAGGATCC TTCCCAGAGG CAAAGGGCAA TGAGCATTGC CAGCATCTTA 
ACAAATACAG TAGAAGAACT AGAAGAATCC AGACAGAAAT GTCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTTTTAAT TTGGGACTGT TCTCCATATT GGCTAAAAGT TAAACATATT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCAA TTACTATCTG TATTGTGTTA 
AATACCCTTT TCATGGCCAT GGAGCACTAC CCCATGACTG AACATTTCAA TCATGTACTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTCACAGCAG AAATGTTCCT GAAAATCATT 
GCCATGGACC CTTACTATTA TTTCCAAGAG GGCTGGAATA TCTTTGATGG TTTCATTGTG 
ACACTCAGCC TGGTAGAACT TGGCCTTGCC AATGTGGAAG GGTTATCAGT TCTCCGTTCA 
TTCCGACTGC TCCGAGTCTT CAAGTTGGCA AAGTCCTGGC CCACACTGAA CATGCTCATT 
AAGATCATCG GCAACTCGGT GGGCGCACTG GGCAACCTGA CCCTGGTGCT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTGTTCGGCA AAAGTTACAA AGATTGTGTC 
TGCAAAATTG CCACTGACTG CAAACTCCCG CGCTGGCACA TGAACGACTT CTTCCACTCC 
TTCCTGATCG TGTTCCGAGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGCATG 
GAGGTCGCGG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGGAAC 
CTTGTGGTCC TGAACCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ACGATGACAA CGAAATGAAC AACCTTCAAA TTGCTGTGGA CAGGATGCAC 
AAAGGAGTAG CTTATGTAAA AAGAAAAATA TATGAGTTTA TTCAACAGTC CTTTGTTAGG 
AAACAGAAGA TCCTAGATGA AATTAAGCCA CTTGATGATC TAAACAACAG AAAAGACAAT 
TGTACATCTA ACCACACGAC AGAGATTGGG AAAGATCTGG ACTGTCTGAA AGATGTGAAT 
GGAACCACGA GTGGCATAGG GACCGGCAGC AGTGTGGAAA AGTACATCAT TGATGAGAGT 
GACTACATGT CATTCATAAA CAACCCCAGC CTCACCGTGA CTGTGCCCAT TGCTGTGGGA 
GAGTCTGACT TTGAAAACTT AAACACAGAG GACTTTAGCA GTGAATCAGA TCTAGAAGAA 
AGCAAAGAGA AACTCAACGA AAGCAGTAGT TCCTCAGAAG GAAGCACAGT AGACATCGGG 
GCACCTGCGG AGGAACAGCC TGTCATGGAA CCAGAAGAAA CCCTTGAGCC CGAAGCTTGC 
TTCACTGAAG GCTGTGTGCA GAGATTCAAG TGCTGTCAAA TCAGTGTGGA AGAAGGAAGA 
GGAAAACAGT GGTGGAACCT TCGGAGAACG TGCTTCCGAA TAGTTGAGCA CAACTGGTTT 
GAGACCTTCA TTGTGTTCAT GATTCTCCTC AGTAGTGGTG CCCTGGCCTT TGAGGATATA 
TACATTGATC AGCGAAAGAC GATCAAGACC ATGCTGGAGT ATGCAGACAA GGTTTTCACT 
TACATTTTTA TCCTGGAGAT GCTCCTCAAA TGGGTAGCCT ACGGCTATCA AACGTATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC TGAACTTGGG GCCATCAAGT CCCTAAGGAC ACTAAGAGCT 
CTGAGACCCC TAAGAGCCTT ATCACGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTG 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GCCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTGT TAACACCACA 
ACTGGTGACA CATTTGAGAT CACCGAAGTC AATAATCATT CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCCCG GTGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTTT CTTTGCTTCA AGTTGCGACG TTTAAGGGTT GGATGGATAT CATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTGCAG CCTAAGTATG AGGAAAGCCT GTACATGTAC 
CTGTACTTCG TCATCTTCAT CATCTTCGGC TCGTTCTTCA CTCTAAATCT ATTCATCGGT 
GTCATCATAG ACAACTTCAA CCAGCAGAAG AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAGAAAT TAGGATCAAA AAAGCCACAA 
AAGCCTATCC CTCGACCTGG AAACAAATTT CAAGGAATGG TCTTTGACTT TGTAACCAGA 
CAAGTGTTTG ACATCAGCAT CATGATCCTC ATCTGTCTGA ACATGGTGAC CATGATGGTG 
GAAACGGATG ACCAGAGCGA TTATGTGACA AGCATTTTGT CACGTATCAA CCTGGTGTTC 
ATTGTGCTGT TCACCGGCGA GTGCGTGCTG AAGCTCATCT CCCTCCGCCA TTATTATTTC 
ACCATTGGCT GGAATATTTT CGATTTTGTG GTGGTCATCC TCTCCATTGT AGGAATGTTT 
CTTGCGGAGC TGATAGAGAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT CATCCGCCTG 
GCCAGGATTG GACGAATCCT ACGCCTGATC AAAGGCGCCA AGGGGATCCG CACTCTGCTC 
TTTGCTCTGA TGATGTCCCT TCCTGCGCTG TTCAACATCG GCCTCCTGCT TTTCCTGGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGA 
ATTGATGACA TGTTCAACTT TGAGACTTTT GGCAACAGCA TGATCTGCCT GTTCCAAATC 
ACCACCTCTG CGGGCTGGGA CGGACTGCTG GCCCCCATCC TCAACAGCAA ACCCCCTGAC 
TGTGACCCTA ATAAAGTTAA CCCCGGAAGC TCGGTGAAGG GGGACTGTGG GAACCCATCC 
GTGGGGATTT TCTTTTTTGT CAGCTACATC ATCATATCCT TCCTGGTGGT GGTGAACATG 
TACATCGCTG TCATCCTGGA GAACTTCAGC GTCGCCACCG AAGAAAGTGC AGAGCCCCTG 
AGTGAGGACG ACTTTGAGAT GTTCTACGAG GTCTGGGAGA AGTTCGACCC TGACGCCACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CTCTCGAACC CCCTCTCAAC 
TTACCACAAC CGAACAAACT TCAGCTCATT GCCATGGACC TGCCCATGGT GAGTGGAGAC 
CGCATCCACT GCCTGGACAT CTTATTTGCT TTTACAAAGC GGGTCCTGGG CGAGAGTGGA 
GAGATGGACG CTCTTCGAAT CCAGATGGAA GAGCGCTTCA TGGCTTCCAA CCCCTCCAAG 
GTCTCCTATC AGCCAATCAC TACAACGTTA AAACGAAAGC AAGAGGAGGT GTCAGCCGTT 
ATCATTCAGC GCGCTTACAG GCGCCACCTT TTGAAGCGAA CAGTAAAACA AGCTTCATTC 
ACATACAATA AGAACAAACT CAAGGGCGGG GCTAATCTTC TTGTAAAAGA AGACATGATC 
ATTGACAGGA TAAATGAAAA CTCTATTACA GAGAAAACTG ACCTGACGAT GTCCACTGCA 
GCTTGTCCGC CCTCCTACGA CCGGGTGACA AAGCCAATCG TGGAGAAACA CGAGCAGGAA 
GGAAAAGATG AGAAAGCCAA AGGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn1a ( XM_008761925.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760147.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X3, mRNA.

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CloneID	ORa43707
Clone ID Related Accession (Same CDS sequence)	XM_008761923.2 , XM_008761925.2 , XM_008761924.1
Accession Version	XM_008761925.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 1 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761925.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGCAG 
GCGGCGGCAG CAACAGCATC AGAACATTCC AGGGAGCCCA GTGCAGCGGG CAGGCTCTCA 
GATAGCTCTT CAGAAGCCTC CAAGTTGAGT TCAAAGAGCG CTAAAGAAAG GCGGAATCGG 
AGGAAAAAAA GAAAACAGAA AGAGCAGTCT GGAGGGGAAG AGAAAGATGA TGATGAATTC 
CACAAATCCG AGTCCGAAGA TAGCATCAGG AGGAAGGGCT TCCGCTTCTC CATAGAAGGG 
AACAGACTGA CCTATGAAAA GAGGTACTCT TCCCCACATC AGTCTCTGTT GAGCATTCGC 
GGCTCACTGT TTTCCCCGAG ACGCAATAGC AGAACAAGTC TTTTCAGCTT CAGAGGGCGA 
GCGAAGGACG TGGGGTCTGA GAATGATTTT GCGGACGATG AGCACAGCAC CTTCGAGGAC 
AATGAGAGCA GGAGAGACTC TCTGTTTGTT CCCCGAAGAC ATGGAGAGCG ACGCAACAGT 
AACCTCAGCC AAACCAGCAG ATCCTCCCGA ATGCTGGCGG GGCTTCCAGC AAACGGGAAG 
ATGCACAGCA CAGTGGATTG CAATGGTGTG GTTTCCTTGG TGGGCGGACC CTCGGTTCCG 
ACATCGCCAG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CTACTGAAAC TGAGATGAGA AAGAGGAGGT CAAGTTCTTT CCATGTTTCC 
ATGGACTTTC TTGAGGATCC TTCCCAGAGG CAAAGGGCAA TGAGCATTGC CAGCATCTTA 
ACAAATACAG TAGAAGAACT AGAAGAATCC AGACAGAAAT GTCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTTTTAAT TTGGGACTGT TCTCCATATT GGCTAAAAGT TAAACATATT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCAA TTACTATCTG TATTGTGTTA 
AATACCCTTT TCATGGCCAT GGAGCACTAC CCCATGACTG AACATTTCAA TCATGTACTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTCACAGCAG AAATGTTCCT GAAAATCATT 
GCCATGGACC CTTACTATTA TTTCCAAGAG GGCTGGAATA TCTTTGATGG TTTCATTGTG 
ACACTCAGCC TGGTAGAACT TGGCCTTGCC AATGTGGAAG GGTTATCAGT TCTCCGTTCA 
TTCCGACTGC TCCGAGTCTT CAAGTTGGCA AAGTCCTGGC CCACACTGAA CATGCTCATT 
AAGATCATCG GCAACTCGGT GGGCGCACTG GGCAACCTGA CCCTGGTGCT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTGTTCGGCA AAAGTTACAA AGATTGTGTC 
TGCAAAATTG CCACTGACTG CAAACTCCCG CGCTGGCACA TGAACGACTT CTTCCACTCC 
TTCCTGATCG TGTTCCGAGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGCATG 
GAGGTCGCGG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGGAAC 
CTTGTGGTCC TGAACCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ACGATGACAA CGAAATGAAC AACCTTCAAA TTGCTGTGGA CAGGATGCAC 
AAAGGAGTAG CTTATGTAAA AAGAAAAATA TATGAGTTTA TTCAACAGTC CTTTGTTAGG 
AAACAGAAGA TCCTAGATGA AATTAAGCCA CTTGATGATC TAAACAACAG AAAAGACAAT 
TGTACATCTA ACCACACGAC AGAGATTGGG AAAGATCTGG ACTGTCTGAA AGATGTGAAT 
GGAACCACGA GTGGCATAGG GACCGGCAGC AGTGTGGAAA AGTACATCAT TGATGAGAGT 
GACTACATGT CATTCATAAA CAACCCCAGC CTCACCGTGA CTGTGCCCAT TGCTGTGGGA 
GAGTCTGACT TTGAAAACTT AAACACAGAG GACTTTAGCA GTGAATCAGA TCTAGAAGAA 
AGCAAAGAGA AACTCAACGA AAGCAGTAGT TCCTCAGAAG GAAGCACAGT AGACATCGGG 
GCACCTGCGG AGGAACAGCC TGTCATGGAA CCAGAAGAAA CCCTTGAGCC CGAAGCTTGC 
TTCACTGAAG GCTGTGTGCA GAGATTCAAG TGCTGTCAAA TCAGTGTGGA AGAAGGAAGA 
GGAAAACAGT GGTGGAACCT TCGGAGAACG TGCTTCCGAA TAGTTGAGCA CAACTGGTTT 
GAGACCTTCA TTGTGTTCAT GATTCTCCTC AGTAGTGGTG CCCTGGCCTT TGAGGATATA 
TACATTGATC AGCGAAAGAC GATCAAGACC ATGCTGGAGT ATGCAGACAA GGTTTTCACT 
TACATTTTTA TCCTGGAGAT GCTCCTCAAA TGGGTAGCCT ACGGCTATCA AACGTATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC TGAACTTGGG GCCATCAAGT CCCTAAGGAC ACTAAGAGCT 
CTGAGACCCC TAAGAGCCTT ATCACGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTG 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GCCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTGT TAACACCACA 
ACTGGTGACA CATTTGAGAT CACCGAAGTC AATAATCATT CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCCCG GTGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTTT CTTTGCTTCA AGTTGCGACG TTTAAGGGTT GGATGGATAT CATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTGCAG CCTAAGTATG AGGAAAGCCT GTACATGTAC 
CTGTACTTCG TCATCTTCAT CATCTTCGGC TCGTTCTTCA CTCTAAATCT ATTCATCGGT 
GTCATCATAG ACAACTTCAA CCAGCAGAAG AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAGAAAT TAGGATCAAA AAAGCCACAA 
AAGCCTATCC CTCGACCTGG AAACAAATTT CAAGGAATGG TCTTTGACTT TGTAACCAGA 
CAAGTGTTTG ACATCAGCAT CATGATCCTC ATCTGTCTGA ACATGGTGAC CATGATGGTG 
GAAACGGATG ACCAGAGCGA TTATGTGACA AGCATTTTGT CACGTATCAA CCTGGTGTTC 
ATTGTGCTGT TCACCGGCGA GTGCGTGCTG AAGCTCATCT CCCTCCGCCA TTATTATTTC 
ACCATTGGCT GGAATATTTT CGATTTTGTG GTGGTCATCC TCTCCATTGT AGGAATGTTT 
CTTGCGGAGC TGATAGAGAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT CATCCGCCTG 
GCCAGGATTG GACGAATCCT ACGCCTGATC AAAGGCGCCA AGGGGATCCG CACTCTGCTC 
TTTGCTCTGA TGATGTCCCT TCCTGCGCTG TTCAACATCG GCCTCCTGCT TTTCCTGGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGA 
ATTGATGACA TGTTCAACTT TGAGACTTTT GGCAACAGCA TGATCTGCCT GTTCCAAATC 
ACCACCTCTG CGGGCTGGGA CGGACTGCTG GCCCCCATCC TCAACAGCAA ACCCCCTGAC 
TGTGACCCTA ATAAAGTTAA CCCCGGAAGC TCGGTGAAGG GGGACTGTGG GAACCCATCC 
GTGGGGATTT TCTTTTTTGT CAGCTACATC ATCATATCCT TCCTGGTGGT GGTGAACATG 
TACATCGCTG TCATCCTGGA GAACTTCAGC GTCGCCACCG AAGAAAGTGC AGAGCCCCTG 
AGTGAGGACG ACTTTGAGAT GTTCTACGAG GTCTGGGAGA AGTTCGACCC TGACGCCACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CTCTCGAACC CCCTCTCAAC 
TTACCACAAC CGAACAAACT TCAGCTCATT GCCATGGACC TGCCCATGGT GAGTGGAGAC 
CGCATCCACT GCCTGGACAT CTTATTTGCT TTTACAAAGC GGGTCCTGGG CGAGAGTGGA 
GAGATGGACG CTCTTCGAAT CCAGATGGAA GAGCGCTTCA TGGCTTCCAA CCCCTCCAAG 
GTCTCCTATC AGCCAATCAC TACAACGTTA AAACGAAAGC AAGAGGAGGT GTCAGCCGTT 
ATCATTCAGC GCGCTTACAG GCGCCACCTT TTGAAGCGAA CAGTAAAACA AGCTTCATTC 
ACATACAATA AGAACAAACT CAAGGGCGGG GCTAATCTTC TTGTAAAAGA AGACATGATC 
ATTGACAGGA TAAATGAAAA CTCTATTACA GAGAAAACTG ACCTGACGAT GTCCACTGCA 
GCTTGTCCGC CCTCCTACGA CCGGGTGACA AAGCCAATCG TGGAGAAACA CGAGCAGGAA 
GGAAAAGATG AGAAAGCCAA AGGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760147.1
CDS	134..6163
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEKNVTTDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFHKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAGLPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCTSNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVMEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDTFEITEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKLKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_008761925.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761925.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGCAG 
GCGGCGGCAG CAACAGCATC AGAACATTCC AGGGAGCCCA GTGCAGCGGG CAGGCTCTCA 
GATAGCTCTT CAGAAGCCTC CAAGTTGAGT TCAAAGAGCG CTAAAGAAAG GCGGAATCGG 
AGGAAAAAAA GAAAACAGAA AGAGCAGTCT GGAGGGGAAG AGAAAGATGA TGATGAATTC 
CACAAATCCG AGTCCGAAGA TAGCATCAGG AGGAAGGGCT TCCGCTTCTC CATAGAAGGG 
AACAGACTGA CCTATGAAAA GAGGTACTCT TCCCCACATC AGTCTCTGTT GAGCATTCGC 
GGCTCACTGT TTTCCCCGAG ACGCAATAGC AGAACAAGTC TTTTCAGCTT CAGAGGGCGA 
GCGAAGGACG TGGGGTCTGA GAATGATTTT GCGGACGATG AGCACAGCAC CTTCGAGGAC 
AATGAGAGCA GGAGAGACTC TCTGTTTGTT CCCCGAAGAC ATGGAGAGCG ACGCAACAGT 
AACCTCAGCC AAACCAGCAG ATCCTCCCGA ATGCTGGCGG GGCTTCCAGC AAACGGGAAG 
ATGCACAGCA CAGTGGATTG CAATGGTGTG GTTTCCTTGG TGGGCGGACC CTCGGTTCCG 
ACATCGCCAG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CTACTGAAAC TGAGATGAGA AAGAGGAGGT CAAGTTCTTT CCATGTTTCC 
ATGGACTTTC TTGAGGATCC TTCCCAGAGG CAAAGGGCAA TGAGCATTGC CAGCATCTTA 
ACAAATACAG TAGAAGAACT AGAAGAATCC AGACAGAAAT GTCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTTTTAAT TTGGGACTGT TCTCCATATT GGCTAAAAGT TAAACATATT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCAA TTACTATCTG TATTGTGTTA 
AATACCCTTT TCATGGCCAT GGAGCACTAC CCCATGACTG AACATTTCAA TCATGTACTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTCACAGCAG AAATGTTCCT GAAAATCATT 
GCCATGGACC CTTACTATTA TTTCCAAGAG GGCTGGAATA TCTTTGATGG TTTCATTGTG 
ACACTCAGCC TGGTAGAACT TGGCCTTGCC AATGTGGAAG GGTTATCAGT TCTCCGTTCA 
TTCCGACTGC TCCGAGTCTT CAAGTTGGCA AAGTCCTGGC CCACACTGAA CATGCTCATT 
AAGATCATCG GCAACTCGGT GGGCGCACTG GGCAACCTGA CCCTGGTGCT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTGTTCGGCA AAAGTTACAA AGATTGTGTC 
TGCAAAATTG CCACTGACTG CAAACTCCCG CGCTGGCACA TGAACGACTT CTTCCACTCC 
TTCCTGATCG TGTTCCGAGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGCATG 
GAGGTCGCGG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGGAAC 
CTTGTGGTCC TGAACCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ACGATGACAA CGAAATGAAC AACCTTCAAA TTGCTGTGGA CAGGATGCAC 
AAAGGAGTAG CTTATGTAAA AAGAAAAATA TATGAGTTTA TTCAACAGTC CTTTGTTAGG 
AAACAGAAGA TCCTAGATGA AATTAAGCCA CTTGATGATC TAAACAACAG AAAAGACAAT 
TGTACATCTA ACCACACGAC AGAGATTGGG AAAGATCTGG ACTGTCTGAA AGATGTGAAT 
GGAACCACGA GTGGCATAGG GACCGGCAGC AGTGTGGAAA AGTACATCAT TGATGAGAGT 
GACTACATGT CATTCATAAA CAACCCCAGC CTCACCGTGA CTGTGCCCAT TGCTGTGGGA 
GAGTCTGACT TTGAAAACTT AAACACAGAG GACTTTAGCA GTGAATCAGA TCTAGAAGAA 
AGCAAAGAGA AACTCAACGA AAGCAGTAGT TCCTCAGAAG GAAGCACAGT AGACATCGGG 
GCACCTGCGG AGGAACAGCC TGTCATGGAA CCAGAAGAAA CCCTTGAGCC CGAAGCTTGC 
TTCACTGAAG GCTGTGTGCA GAGATTCAAG TGCTGTCAAA TCAGTGTGGA AGAAGGAAGA 
GGAAAACAGT GGTGGAACCT TCGGAGAACG TGCTTCCGAA TAGTTGAGCA CAACTGGTTT 
GAGACCTTCA TTGTGTTCAT GATTCTCCTC AGTAGTGGTG CCCTGGCCTT TGAGGATATA 
TACATTGATC AGCGAAAGAC GATCAAGACC ATGCTGGAGT ATGCAGACAA GGTTTTCACT 
TACATTTTTA TCCTGGAGAT GCTCCTCAAA TGGGTAGCCT ACGGCTATCA AACGTATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC TGAACTTGGG GCCATCAAGT CCCTAAGGAC ACTAAGAGCT 
CTGAGACCCC TAAGAGCCTT ATCACGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTG 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GCCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTGT TAACACCACA 
ACTGGTGACA CATTTGAGAT CACCGAAGTC AATAATCATT CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCCCG GTGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTTT CTTTGCTTCA AGTTGCGACG TTTAAGGGTT GGATGGATAT CATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTGCAG CCTAAGTATG AGGAAAGCCT GTACATGTAC 
CTGTACTTCG TCATCTTCAT CATCTTCGGC TCGTTCTTCA CTCTAAATCT ATTCATCGGT 
GTCATCATAG ACAACTTCAA CCAGCAGAAG AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAGAAAT TAGGATCAAA AAAGCCACAA 
AAGCCTATCC CTCGACCTGG AAACAAATTT CAAGGAATGG TCTTTGACTT TGTAACCAGA 
CAAGTGTTTG ACATCAGCAT CATGATCCTC ATCTGTCTGA ACATGGTGAC CATGATGGTG 
GAAACGGATG ACCAGAGCGA TTATGTGACA AGCATTTTGT CACGTATCAA CCTGGTGTTC 
ATTGTGCTGT TCACCGGCGA GTGCGTGCTG AAGCTCATCT CCCTCCGCCA TTATTATTTC 
ACCATTGGCT GGAATATTTT CGATTTTGTG GTGGTCATCC TCTCCATTGT AGGAATGTTT 
CTTGCGGAGC TGATAGAGAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT CATCCGCCTG 
GCCAGGATTG GACGAATCCT ACGCCTGATC AAAGGCGCCA AGGGGATCCG CACTCTGCTC 
TTTGCTCTGA TGATGTCCCT TCCTGCGCTG TTCAACATCG GCCTCCTGCT TTTCCTGGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGA 
ATTGATGACA TGTTCAACTT TGAGACTTTT GGCAACAGCA TGATCTGCCT GTTCCAAATC 
ACCACCTCTG CGGGCTGGGA CGGACTGCTG GCCCCCATCC TCAACAGCAA ACCCCCTGAC 
TGTGACCCTA ATAAAGTTAA CCCCGGAAGC TCGGTGAAGG GGGACTGTGG GAACCCATCC 
GTGGGGATTT TCTTTTTTGT CAGCTACATC ATCATATCCT TCCTGGTGGT GGTGAACATG 
TACATCGCTG TCATCCTGGA GAACTTCAGC GTCGCCACCG AAGAAAGTGC AGAGCCCCTG 
AGTGAGGACG ACTTTGAGAT GTTCTACGAG GTCTGGGAGA AGTTCGACCC TGACGCCACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CTCTCGAACC CCCTCTCAAC 
TTACCACAAC CGAACAAACT TCAGCTCATT GCCATGGACC TGCCCATGGT GAGTGGAGAC 
CGCATCCACT GCCTGGACAT CTTATTTGCT TTTACAAAGC GGGTCCTGGG CGAGAGTGGA 
GAGATGGACG CTCTTCGAAT CCAGATGGAA GAGCGCTTCA TGGCTTCCAA CCCCTCCAAG 
GTCTCCTATC AGCCAATCAC TACAACGTTA AAACGAAAGC AAGAGGAGGT GTCAGCCGTT 
ATCATTCAGC GCGCTTACAG GCGCCACCTT TTGAAGCGAA CAGTAAAACA AGCTTCATTC 
ACATACAATA AGAACAAACT CAAGGGCGGG GCTAATCTTC TTGTAAAAGA AGACATGATC 
ATTGACAGGA TAAATGAAAA CTCTATTACA GAGAAAACTG ACCTGACGAT GTCCACTGCA 
GCTTGTCCGC CCTCCTACGA CCGGGTGACA AAGCCAATCG TGGAGAAACA CGAGCAGGAA 
GGAAAAGATG AGAAAGCCAA AGGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn1a ( XM_008761924.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760146.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X1, mRNA.

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CloneID	ORa43707
Clone ID Related Accession (Same CDS sequence)	XM_008761923.2 , XM_008761925.2 , XM_008761924.1
Accession Version	XM_008761924.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 1 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGCAG 
GCGGCGGCAG CAACAGCATC AGAACATTCC AGGGAGCCCA GTGCAGCGGG CAGGCTCTCA 
GATAGCTCTT CAGAAGCCTC CAAGTTGAGT TCAAAGAGCG CTAAAGAAAG GCGGAATCGG 
AGGAAAAAAA GAAAACAGAA AGAGCAGTCT GGAGGGGAAG AGAAAGATGA TGATGAATTC 
CACAAATCCG AGTCCGAAGA TAGCATCAGG AGGAAGGGCT TCCGCTTCTC CATAGAAGGG 
AACAGACTGA CCTATGAAAA GAGGTACTCT TCCCCACATC AGTCTCTGTT GAGCATTCGC 
GGCTCACTGT TTTCCCCGAG ACGCAATAGC AGAACAAGTC TTTTCAGCTT CAGAGGGCGA 
GCGAAGGACG TGGGGTCTGA GAATGATTTT GCGGACGATG AGCACAGCAC CTTCGAGGAC 
AATGAGAGCA GGAGAGACTC TCTGTTTGTT CCCCGAAGAC ATGGAGAGCG ACGCAACAGT 
AACCTCAGCC AAACCAGCAG ATCCTCCCGA ATGCTGGCGG GGCTTCCAGC AAACGGGAAG 
ATGCACAGCA CAGTGGATTG CAATGGTGTG GTTTCCTTGG TGGGCGGACC CTCGGTTCCG 
ACATCGCCAG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CTACTGAAAC TGAGATGAGA AAGAGGAGGT CAAGTTCTTT CCATGTTTCC 
ATGGACTTTC TTGAGGATCC TTCCCAGAGG CAAAGGGCAA TGAGCATTGC CAGCATCTTA 
ACAAATACAG TAGAAGAACT AGAAGAATCC AGACAGAAAT GTCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTTTTAAT TTGGGACTGT TCTCCATATT GGCTAAAAGT TAAACATATT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCAA TTACTATCTG TATTGTGTTA 
AATACCCTTT TCATGGCCAT GGAGCACTAC CCCATGACTG AACATTTCAA TCATGTACTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTCACAGCAG AAATGTTCCT GAAAATCATT 
GCCATGGACC CTTACTATTA TTTCCAAGAG GGCTGGAATA TCTTTGATGG TTTCATTGTG 
ACACTCAGCC TGGTAGAACT TGGCCTTGCC AATGTGGAAG GGTTATCAGT TCTCCGTTCA 
TTCCGACTGC TCCGAGTCTT CAAGTTGGCA AAGTCCTGGC CCACACTGAA CATGCTCATT 
AAGATCATCG GCAACTCGGT GGGCGCACTG GGCAACCTGA CCCTGGTGCT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTGTTCGGCA AAAGTTACAA AGATTGTGTC 
TGCAAAATTG CCACTGACTG CAAACTCCCG CGCTGGCACA TGAACGACTT CTTCCACTCC 
TTCCTGATCG TGTTCCGAGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGCATG 
GAGGTCGCGG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGGAAC 
CTTGTGGTCC TGAACCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ACGATGACAA CGAAATGAAC AACCTTCAAA TTGCTGTGGA CAGGATGCAC 
AAAGGAGTAG CTTATGTAAA AAGAAAAATA TATGAGTTTA TTCAACAGTC CTTTGTTAGG 
AAACAGAAGA TCCTAGATGA AATTAAGCCA CTTGATGATC TAAACAACAG AAAAGACAAT 
TGTACATCTA ACCACACGAC AGAGATTGGG AAAGATCTGG ACTGTCTGAA AGATGTGAAT 
GGAACCACGA GTGGCATAGG GACCGGCAGC AGTGTGGAAA AGTACATCAT TGATGAGAGT 
GACTACATGT CATTCATAAA CAACCCCAGC CTCACCGTGA CTGTGCCCAT TGCTGTGGGA 
GAGTCTGACT TTGAAAACTT AAACACAGAG GACTTTAGCA GTGAATCAGA TCTAGAAGAA 
AGCAAAGAGA AACTCAACGA AAGCAGTAGT TCCTCAGAAG GAAGCACAGT AGACATCGGG 
GCACCTGCGG AGGAACAGCC TGTCATGGAA CCAGAAGAAA CCCTTGAGCC CGAAGCTTGC 
TTCACTGAAG GCTGTGTGCA GAGATTCAAG TGCTGTCAAA TCAGTGTGGA AGAAGGAAGA 
GGAAAACAGT GGTGGAACCT TCGGAGAACG TGCTTCCGAA TAGTTGAGCA CAACTGGTTT 
GAGACCTTCA TTGTGTTCAT GATTCTCCTC AGTAGTGGTG CCCTGGCCTT TGAGGATATA 
TACATTGATC AGCGAAAGAC GATCAAGACC ATGCTGGAGT ATGCAGACAA GGTTTTCACT 
TACATTTTTA TCCTGGAGAT GCTCCTCAAA TGGGTAGCCT ACGGCTATCA AACGTATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC TGAACTTGGG GCCATCAAGT CCCTAAGGAC ACTAAGAGCT 
CTGAGACCCC TAAGAGCCTT ATCACGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTG 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GCCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTGT TAACACCACA 
ACTGGTGACA CATTTGAGAT CACCGAAGTC AATAATCATT CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCCCG GTGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTTT CTTTGCTTCA AGTTGCGACG TTTAAGGGTT GGATGGATAT CATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTGCAG CCTAAGTATG AGGAAAGCCT GTACATGTAC 
CTGTACTTCG TCATCTTCAT CATCTTCGGC TCGTTCTTCA CTCTAAATCT ATTCATCGGT 
GTCATCATAG ACAACTTCAA CCAGCAGAAG AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAGAAAT TAGGATCAAA AAAGCCACAA 
AAGCCTATCC CTCGACCTGG AAACAAATTT CAAGGAATGG TCTTTGACTT TGTAACCAGA 
CAAGTGTTTG ACATCAGCAT CATGATCCTC ATCTGTCTGA ACATGGTGAC CATGATGGTG 
GAAACGGATG ACCAGAGCGA TTATGTGACA AGCATTTTGT CACGTATCAA CCTGGTGTTC 
ATTGTGCTGT TCACCGGCGA GTGCGTGCTG AAGCTCATCT CCCTCCGCCA TTATTATTTC 
ACCATTGGCT GGAATATTTT CGATTTTGTG GTGGTCATCC TCTCCATTGT AGGAATGTTT 
CTTGCGGAGC TGATAGAGAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT CATCCGCCTG 
GCCAGGATTG GACGAATCCT ACGCCTGATC AAAGGCGCCA AGGGGATCCG CACTCTGCTC 
TTTGCTCTGA TGATGTCCCT TCCTGCGCTG TTCAACATCG GCCTCCTGCT TTTCCTGGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGA 
ATTGATGACA TGTTCAACTT TGAGACTTTT GGCAACAGCA TGATCTGCCT GTTCCAAATC 
ACCACCTCTG CGGGCTGGGA CGGACTGCTG GCCCCCATCC TCAACAGCAA ACCCCCTGAC 
TGTGACCCTA ATAAAGTTAA CCCCGGAAGC TCGGTGAAGG GGGACTGTGG GAACCCATCC 
GTGGGGATTT TCTTTTTTGT CAGCTACATC ATCATATCCT TCCTGGTGGT GGTGAACATG 
TACATCGCTG TCATCCTGGA GAACTTCAGC GTCGCCACCG AAGAAAGTGC AGAGCCCCTG 
AGTGAGGACG ACTTTGAGAT GTTCTACGAG GTCTGGGAGA AGTTCGACCC TGACGCCACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CTCTCGAACC CCCTCTCAAC 
TTACCACAAC CGAACAAACT TCAGCTCATT GCCATGGACC TGCCCATGGT GAGTGGAGAC 
CGCATCCACT GCCTGGACAT CTTATTTGCT TTTACAAAGC GGGTCCTGGG CGAGAGTGGA 
GAGATGGACG CTCTTCGAAT CCAGATGGAA GAGCGCTTCA TGGCTTCCAA CCCCTCCAAG 
GTCTCCTATC AGCCAATCAC TACAACGTTA AAACGAAAGC AAGAGGAGGT GTCAGCCGTT 
ATCATTCAGC GCGCTTACAG GCGCCACCTT TTGAAGCGAA CAGTAAAACA AGCTTCATTC 
ACATACAATA AGAACAAACT CAAGGGCGGG GCTAATCTTC TTGTAAAAGA AGACATGATC 
ATTGACAGGA TAAATGAAAA CTCTATTACA GAGAAAACTG ACCTGACGAT GTCCACTGCA 
GCTTGTCCGC CCTCCTACGA CCGGGTGACA AAGCCAATCG TGGAGAAACA CGAGCAGGAA 
GGAAAAGATG AGAAAGCCAA AGGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760146.1
CDS	180..6209
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEKNVTTDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQQAAAATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFHKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAGLPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCTSNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVMEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDTFEITEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKLKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_008761924.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761924.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGCAG 
GCGGCGGCAG CAACAGCATC AGAACATTCC AGGGAGCCCA GTGCAGCGGG CAGGCTCTCA 
GATAGCTCTT CAGAAGCCTC CAAGTTGAGT TCAAAGAGCG CTAAAGAAAG GCGGAATCGG 
AGGAAAAAAA GAAAACAGAA AGAGCAGTCT GGAGGGGAAG AGAAAGATGA TGATGAATTC 
CACAAATCCG AGTCCGAAGA TAGCATCAGG AGGAAGGGCT TCCGCTTCTC CATAGAAGGG 
AACAGACTGA CCTATGAAAA GAGGTACTCT TCCCCACATC AGTCTCTGTT GAGCATTCGC 
GGCTCACTGT TTTCCCCGAG ACGCAATAGC AGAACAAGTC TTTTCAGCTT CAGAGGGCGA 
GCGAAGGACG TGGGGTCTGA GAATGATTTT GCGGACGATG AGCACAGCAC CTTCGAGGAC 
AATGAGAGCA GGAGAGACTC TCTGTTTGTT CCCCGAAGAC ATGGAGAGCG ACGCAACAGT 
AACCTCAGCC AAACCAGCAG ATCCTCCCGA ATGCTGGCGG GGCTTCCAGC AAACGGGAAG 
ATGCACAGCA CAGTGGATTG CAATGGTGTG GTTTCCTTGG TGGGCGGACC CTCGGTTCCG 
ACATCGCCAG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CTACTGAAAC TGAGATGAGA AAGAGGAGGT CAAGTTCTTT CCATGTTTCC 
ATGGACTTTC TTGAGGATCC TTCCCAGAGG CAAAGGGCAA TGAGCATTGC CAGCATCTTA 
ACAAATACAG TAGAAGAACT AGAAGAATCC AGACAGAAAT GTCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTTTTAAT TTGGGACTGT TCTCCATATT GGCTAAAAGT TAAACATATT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCAA TTACTATCTG TATTGTGTTA 
AATACCCTTT TCATGGCCAT GGAGCACTAC CCCATGACTG AACATTTCAA TCATGTACTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTCACAGCAG AAATGTTCCT GAAAATCATT 
GCCATGGACC CTTACTATTA TTTCCAAGAG GGCTGGAATA TCTTTGATGG TTTCATTGTG 
ACACTCAGCC TGGTAGAACT TGGCCTTGCC AATGTGGAAG GGTTATCAGT TCTCCGTTCA 
TTCCGACTGC TCCGAGTCTT CAAGTTGGCA AAGTCCTGGC CCACACTGAA CATGCTCATT 
AAGATCATCG GCAACTCGGT GGGCGCACTG GGCAACCTGA CCCTGGTGCT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTGTTCGGCA AAAGTTACAA AGATTGTGTC 
TGCAAAATTG CCACTGACTG CAAACTCCCG CGCTGGCACA TGAACGACTT CTTCCACTCC 
TTCCTGATCG TGTTCCGAGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGCATG 
GAGGTCGCGG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGGAAC 
CTTGTGGTCC TGAACCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ACGATGACAA CGAAATGAAC AACCTTCAAA TTGCTGTGGA CAGGATGCAC 
AAAGGAGTAG CTTATGTAAA AAGAAAAATA TATGAGTTTA TTCAACAGTC CTTTGTTAGG 
AAACAGAAGA TCCTAGATGA AATTAAGCCA CTTGATGATC TAAACAACAG AAAAGACAAT 
TGTACATCTA ACCACACGAC AGAGATTGGG AAAGATCTGG ACTGTCTGAA AGATGTGAAT 
GGAACCACGA GTGGCATAGG GACCGGCAGC AGTGTGGAAA AGTACATCAT TGATGAGAGT 
GACTACATGT CATTCATAAA CAACCCCAGC CTCACCGTGA CTGTGCCCAT TGCTGTGGGA 
GAGTCTGACT TTGAAAACTT AAACACAGAG GACTTTAGCA GTGAATCAGA TCTAGAAGAA 
AGCAAAGAGA AACTCAACGA AAGCAGTAGT TCCTCAGAAG GAAGCACAGT AGACATCGGG 
GCACCTGCGG AGGAACAGCC TGTCATGGAA CCAGAAGAAA CCCTTGAGCC CGAAGCTTGC 
TTCACTGAAG GCTGTGTGCA GAGATTCAAG TGCTGTCAAA TCAGTGTGGA AGAAGGAAGA 
GGAAAACAGT GGTGGAACCT TCGGAGAACG TGCTTCCGAA TAGTTGAGCA CAACTGGTTT 
GAGACCTTCA TTGTGTTCAT GATTCTCCTC AGTAGTGGTG CCCTGGCCTT TGAGGATATA 
TACATTGATC AGCGAAAGAC GATCAAGACC ATGCTGGAGT ATGCAGACAA GGTTTTCACT 
TACATTTTTA TCCTGGAGAT GCTCCTCAAA TGGGTAGCCT ACGGCTATCA AACGTATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC TGAACTTGGG GCCATCAAGT CCCTAAGGAC ACTAAGAGCT 
CTGAGACCCC TAAGAGCCTT ATCACGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTG 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GCCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTGT TAACACCACA 
ACTGGTGACA CATTTGAGAT CACCGAAGTC AATAATCATT CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCCCG GTGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTTT CTTTGCTTCA AGTTGCGACG TTTAAGGGTT GGATGGATAT CATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTGCAG CCTAAGTATG AGGAAAGCCT GTACATGTAC 
CTGTACTTCG TCATCTTCAT CATCTTCGGC TCGTTCTTCA CTCTAAATCT ATTCATCGGT 
GTCATCATAG ACAACTTCAA CCAGCAGAAG AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAGAAAT TAGGATCAAA AAAGCCACAA 
AAGCCTATCC CTCGACCTGG AAACAAATTT CAAGGAATGG TCTTTGACTT TGTAACCAGA 
CAAGTGTTTG ACATCAGCAT CATGATCCTC ATCTGTCTGA ACATGGTGAC CATGATGGTG 
GAAACGGATG ACCAGAGCGA TTATGTGACA AGCATTTTGT CACGTATCAA CCTGGTGTTC 
ATTGTGCTGT TCACCGGCGA GTGCGTGCTG AAGCTCATCT CCCTCCGCCA TTATTATTTC 
ACCATTGGCT GGAATATTTT CGATTTTGTG GTGGTCATCC TCTCCATTGT AGGAATGTTT 
CTTGCGGAGC TGATAGAGAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT CATCCGCCTG 
GCCAGGATTG GACGAATCCT ACGCCTGATC AAAGGCGCCA AGGGGATCCG CACTCTGCTC 
TTTGCTCTGA TGATGTCCCT TCCTGCGCTG TTCAACATCG GCCTCCTGCT TTTCCTGGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGA 
ATTGATGACA TGTTCAACTT TGAGACTTTT GGCAACAGCA TGATCTGCCT GTTCCAAATC 
ACCACCTCTG CGGGCTGGGA CGGACTGCTG GCCCCCATCC TCAACAGCAA ACCCCCTGAC 
TGTGACCCTA ATAAAGTTAA CCCCGGAAGC TCGGTGAAGG GGGACTGTGG GAACCCATCC 
GTGGGGATTT TCTTTTTTGT CAGCTACATC ATCATATCCT TCCTGGTGGT GGTGAACATG 
TACATCGCTG TCATCCTGGA GAACTTCAGC GTCGCCACCG AAGAAAGTGC AGAGCCCCTG 
AGTGAGGACG ACTTTGAGAT GTTCTACGAG GTCTGGGAGA AGTTCGACCC TGACGCCACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CTCTCGAACC CCCTCTCAAC 
TTACCACAAC CGAACAAACT TCAGCTCATT GCCATGGACC TGCCCATGGT GAGTGGAGAC 
CGCATCCACT GCCTGGACAT CTTATTTGCT TTTACAAAGC GGGTCCTGGG CGAGAGTGGA 
GAGATGGACG CTCTTCGAAT CCAGATGGAA GAGCGCTTCA TGGCTTCCAA CCCCTCCAAG 
GTCTCCTATC AGCCAATCAC TACAACGTTA AAACGAAAGC AAGAGGAGGT GTCAGCCGTT 
ATCATTCAGC GCGCTTACAG GCGCCACCTT TTGAAGCGAA CAGTAAAACA AGCTTCATTC 
ACATACAATA AGAACAAACT CAAGGGCGGG GCTAATCTTC TTGTAAAAGA AGACATGATC 
ATTGACAGGA TAAATGAAAA CTCTATTACA GAGAAAACTG ACCTGACGAT GTCCACTGCA 
GCTTGTCCGC CCTCCTACGA CCGGGTGACA AAGCCAATCG TGGAGAAACA CGAGCAGGAA 
GGAAAAGATG AGAAAGCCAA AGGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Scn1a ( XM_017592055.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447544.1 Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X5, mRNA.

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CloneID	ORa43708
Clone ID Related Accession (Same CDS sequence)	XM_017592055.1
Accession Version	XM_017592055.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6027bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sodium channel protein type 1 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGGCG 
GCGGCAGCAA CAGCATCAGA ACATTCCAGG GAGCCCAGTG CAGCGGGCAG GCTCTCAGAT 
AGCTCTTCAG AAGCCTCCAA GTTGAGTTCA AAGAGCGCTA AAGAAAGGCG GAATCGGAGG 
AAAAAAAGAA AACAGAAAGA GCAGTCTGGA GGGGAAGAGA AAGATGATGA TGAATTCCAC 
AAATCCGAGT CCGAAGATAG CATCAGGAGG AAGGGCTTCC GCTTCTCCAT AGAAGGGAAC 
AGACTGACCT ATGAAAAGAG GTACTCTTCC CCACATCAGT CTCTGTTGAG CATTCGCGGC 
TCACTGTTTT CCCCGAGACG CAATAGCAGA ACAAGTCTTT TCAGCTTCAG AGGGCGAGCG 
AAGGACGTGG GGTCTGAGAA TGATTTTGCG GACGATGAGC ACAGCACCTT CGAGGACAAT 
GAGAGCAGGA GAGACTCTCT GTTTGTTCCC CGAAGACATG GAGAGCGACG CAACAGTAAC 
CTCAGCCAAA CCAGCAGATC CTCCCGAATG CTGGCGGGGC TTCCAGCAAA CGGGAAGATG 
CACAGCACAG TGGATTGCAA TGGTGTGGTT TCCTTGGTGG GCGGACCCTC GGTTCCGACA 
TCGCCAGTTG GACAGCTTCT GCCAGAGGTG ATAATAGATA AGCCAGCTAC TGATGACAAT 
GGAACAACTA CTGAAACTGA GATGAGAAAG AGGAGGTCAA GTTCTTTCCA TGTTTCCATG 
GACTTTCTTG AGGATCCTTC CCAGAGGCAA AGGGCAATGA GCATTGCCAG CATCTTAACA 
AATACAGTAG AAGAACTAGA AGAATCCAGA CAGAAATGTC CACCCTGTTG GTATAAATTT 
TCCAACATAT TTTTAATTTG GGACTGTTCT CCATATTGGC TAAAAGTTAA ACATATTGTC 
AACCTGGTTG TGATGGACCC ATTTGTTGAC CTGGCAATTA CTATCTGTAT TGTGTTAAAT 
ACCCTTTTCA TGGCCATGGA GCACTACCCC ATGACTGAAC ATTTCAATCA TGTACTTACA 
GTAGGAAACT TGGTTTTCAC TGGGATCTTC ACAGCAGAAA TGTTCCTGAA AATCATTGCC 
ATGGACCCTT ACTATTATTT CCAAGAGGGC TGGAATATCT TTGATGGTTT CATTGTGACA 
CTCAGCCTGG TAGAACTTGG CCTTGCCAAT GTGGAAGGGT TATCAGTTCT CCGTTCATTC 
CGACTGCTCC GAGTCTTCAA GTTGGCAAAG TCCTGGCCCA CACTGAACAT GCTCATTAAG 
ATCATCGGCA ACTCGGTGGG CGCACTGGGC AACCTGACCC TGGTGCTGGC CATCATCGTC 
TTCATTTTTG CCGTGGTCGG CATGCAGCTG TTCGGCAAAA GTTACAAAGA TTGTGTCTGC 
AAAATTGCCA CTGACTGCAA ACTCCCGCGC TGGCACATGA ACGACTTCTT CCACTCCTTC 
CTGATCGTGT TCCGAGTGCT GTGTGGGGAG TGGATAGAGA CCATGTGGGA CTGCATGGAG 
GTCGCGGGTC AAGCCATGTG CCTTACTGTC TTCATGATGG TCATGGTGAT TGGGAACCTT 
GTGGTCCTGA ACCTCTTTCT GGCCTTGCTT CTGAGCTCAT TTAGTGCAGA CAACCTTGCA 
GCCACTGACG ATGACAACGA AATGAACAAC CTTCAAATTG CTGTGGACAG GATGCACAAA 
GGAGTAGCTT ATGTAAAAAG AAAAATATAT GAGTTTATTC AACAGTCCTT TGTTAGGAAA 
CAGAAGATCC TAGATGAAAT TAAGCCACTT GATGATCTAA ACAACAGAAA AGACAATTGT 
ACATCTAACC ACACGACAGA GATTGGGAAA GATCTGGACT GTCTGAAAGA TGTGAATGGA 
ACCACGAGTG GCATAGGGAC CGGCAGCAGT GTGGAAAAGT ACATCATTGA TGAGAGTGAC 
TACATGTCAT TCATAAACAA CCCCAGCCTC ACCGTGACTG TGCCCATTGC TGTGGGAGAG 
TCTGACTTTG AAAACTTAAA CACAGAGGAC TTTAGCAGTG AATCAGATCT AGAAGAAAGC 
AAAGAGAAAC TCAACGAAAG CAGTAGTTCC TCAGAAGGAA GCACAGTAGA CATCGGGGCA 
CCTGCGGAGG AACAGCCTGT CATGGAACCA GAAGAAACCC TTGAGCCCGA AGCTTGCTTC 
ACTGAAGGCT GTGTGCAGAG ATTCAAGTGC TGTCAAATCA GTGTGGAAGA AGGAAGAGGA 
AAACAGTGGT GGAACCTTCG GAGAACGTGC TTCCGAATAG TTGAGCACAA CTGGTTTGAG 
ACCTTCATTG TGTTCATGAT TCTCCTCAGT AGTGGTGCCC TGGCCTTTGA GGATATATAC 
ATTGATCAGC GAAAGACGAT CAAGACCATG CTGGAGTATG CAGACAAGGT TTTCACTTAC 
ATTTTTATCC TGGAGATGCT CCTCAAATGG GTAGCCTACG GCTATCAAAC GTATTTCACC 
AATGCCTGGT GTTGGCTGGA CTTCTTAATT GTTGATGTTT CATTGGTCAG TTTAACAGCA 
AATGCCTTGG GTTACTCTGA ACTTGGGGCC ATCAAGTCCC TAAGGACACT AAGAGCTCTG 
AGACCCCTAA GAGCCTTATC ACGATTTGAA GGGATGAGGG TGGTTGTGAA TGCCCTGTTA 
GGAGCAATTC CATCCATCAT GAATGTGCTT CTGGTTTGCC TTATATTCTG GCTAATTTTC 
AGCATCATGG GCGTAAATTT GTTTGCTGGC AAATTCTACC ACTGTGTTAA CACCACAACT 
GGTGACACAT TTGAGATCAC CGAAGTCAAT AATCATTCTG ATTGCCTAAA ACTAATAGAA 
AGAAATGAGA CTGCCCGGTG GAAAAATGTG AAAGTAAACT TTGATAATGT AGGATTTGGG 
TATCTTTCTT TGCTTCAAGT TGCGACGTTT AAGGGTTGGA TGGATATCAT GTATGCAGCA 
GTTGATTCCA GAAATGTGGA ACTGCAGCCT AAGTATGAGG AAAGCCTGTA CATGTACCTG 
TACTTCGTCA TCTTCATCAT CTTCGGCTCG TTCTTCACTC TAAATCTATT CATCGGTGTC 
ATCATAGACA ACTTCAACCA GCAGAAGAAG AAGTTTGGAG GTCAAGACAT CTTTATGACA 
GAAGAACAGA AGAAATACTA TAATGCAATG AAGAAATTAG GATCAAAAAA GCCACAAAAG 
CCTATCCCTC GACCTGGAAA CAAATTTCAA GGAATGGTCT TTGACTTTGT AACCAGACAA 
GTGTTTGACA TCAGCATCAT GATCCTCATC TGTCTGAACA TGGTGACCAT GATGGTGGAA 
ACGGATGACC AGAGCGATTA TGTGACAAGC ATTTTGTCAC GTATCAACCT GGTGTTCATT 
GTGCTGTTCA CCGGCGAGTG CGTGCTGAAG CTCATCTCCC TCCGCCATTA TTATTTCACC 
ATTGGCTGGA ATATTTTCGA TTTTGTGGTG GTCATCCTCT CCATTGTAGG AATGTTTCTT 
GCGGAGCTGA TAGAGAAGTA TTTCGTGTCC CCTACCCTGT TCCGAGTCAT CCGCCTGGCC 
AGGATTGGAC GAATCCTACG CCTGATCAAA GGCGCCAAGG GGATCCGCAC TCTGCTCTTT 
GCTCTGATGA TGTCCCTTCC TGCGCTGTTC AACATCGGCC TCCTGCTTTT CCTGGTCATG 
TTCATCTACG CCATCTTTGG GATGTCCAAC TTTGCCTATG TTAAGAGGGA AGTTGGAATT 
GATGACATGT TCAACTTTGA GACTTTTGGC AACAGCATGA TCTGCCTGTT CCAAATCACC 
ACCTCTGCGG GCTGGGACGG ACTGCTGGCC CCCATCCTCA ACAGCAAACC CCCTGACTGT 
GACCCTAATA AAGTTAACCC CGGAAGCTCG GTGAAGGGGG ACTGTGGGAA CCCATCCGTG 
GGGATTTTCT TTTTTGTCAG CTACATCATC ATATCCTTCC TGGTGGTGGT GAACATGTAC 
ATCGCTGTCA TCCTGGAGAA CTTCAGCGTC GCCACCGAAG AAAGTGCAGA GCCCCTGAGT 
GAGGACGACT TTGAGATGTT CTACGAGGTC TGGGAGAAGT TCGACCCTGA CGCCACTCAG 
TTCATGGAAT TTGAAAAATT ATCTCAGTTT GCAGCTGCTC TCGAACCCCC TCTCAACTTA 
CCACAACCGA ACAAACTTCA GCTCATTGCC ATGGACCTGC CCATGGTGAG TGGAGACCGC 
ATCCACTGCC TGGACATCTT ATTTGCTTTT ACAAAGCGGG TCCTGGGCGA GAGTGGAGAG 
ATGGACGCTC TTCGAATCCA GATGGAAGAG CGCTTCATGG CTTCCAACCC CTCCAAGGTC 
TCCTATCAGC CAATCACTAC AACGTTAAAA CGAAAGCAAG AGGAGGTGTC AGCCGTTATC 
ATTCAGCGCG CTTACAGGCG CCACCTTTTG AAGCGAACAG TAAAACAAGC TTCATTCACA 
TACAATAAGA ACAAACTCAA GGGCGGGGCT AATCTTCTTG TAAAAGAAGA CATGATCATT 
GACAGGATAA ATGAAAACTC TATTACAGAG AAAACTGACC TGACGATGTC CACTGCAGCT 
TGTCCGCCCT CCTACGACCG GGTGACAAAG CCAATCGTGG AGAAACACGA GCAGGAAGGA 
AAAGATGAGA AAGCCAAAGG GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447544.1
CDS	180..6206
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCVQWPPTNASLEEHSIEKNVTTDYNGTLVNETVFEFDWKSYIQDSRYHYFLEGVLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMLEQLKKQQEAAQAAAATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDDDEFHKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAGLPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHIVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTEHFNHVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIATDCKLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFVRKQKILDEIKPLDDLNNRKDNCTSNHTTEIGKDLDCLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPAEEQPVMEPEETLEPEACFTEGCVQRFKCCQISVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCVNTTTGDTFEITEVNNHSDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSDYVTSILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKLKGGANLLVKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017592055.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sodium voltage-gated channel alpha subunit 1 (Scn1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017592055.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCAG CTATTGAAAG GCGCATTGCA GAAGAAAAAG CTAAGAATCC CAAGCCAGAC 
AAAAAAGATG ATGATGAAAA TGGCCCAAAG CCGAACAGTG ACTTGGAAGC TGGGAAGAAC 
CTTCCATTTA TCTATGGAGA CATTCCTCCA GAGATGGTGT CGGAGCCTCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACTTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA CTCATTATTC AGCATGTTAA TTATGTGCAC TATTTTGACG 
AACTGTGTAT TTATGACAAT GAGTAACCCT CCTGACTGGA CAAAGAATGT AGAGTACACC 
TTCACAGGAA TATATACCTT TGAATCACTA ATAAAAATTA TTGCAAGGGG CTTCTGTTTA 
GAAGATTTTA CTTTCCTTCG TGACCCATGG AACTGGCTGG ACTTCACTGT CATTACATTC 
GCATATGTGA CGGAGTTTGT GGACCTGGGC AATGTCTCAG CGTTGAGAAC ATTCAGAGTT 
CTTCGAGCAT TGAAAACAAT ATCAGTCATT CCAGGCCTGA AGACCATCGT GGGGGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCTGACGTC ATGATCCTCA CCGTGTTCTG TCTCAGTGTG 
TTTGCTCTAA TCGGGTTGCA GCTTTTCATG GGCAACCTGA GGAATAAGTG TGTACAGTGG 
CCCCCCACCA ACGCTTCCCT TGAGGAACAT AGCATAGAGA AGAATGTAAC TACGGATTAC 
AACGGCACAC TTGTAAATGA AACCGTGTTT GAATTTGACT GGAAATCATA CATTCAAGAC 
TCAAGATATC ATTATTTCCT GGAGGGTGTT TTAGATGCAC TACTGTGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA AGGTTACATG TGTGTAAAAG CTGGCAGAAA CCCTAACTAT 
GGTTACACAA GCTTTGACAC CTTCAGCTGG GCATTTCTGT CCCTGTTTCG ACTGATGACT 
CAGGACTTCT GGGAAAATCT TTACCAACTG ACATTGCGTG CTGCCGGGAA AACATACATG 
ATATTTTTTG TGCTGGTCAT TTTCTTGGGC TCATTCTACC TGATAAACTT GATCCTGGCT 
GTGGTGGCCA TGGCCTATGA GGAACAGAAC CAGGCCACAT TGGAGGAGGC TGAACAGAAG 
GAGGCAGAGT TTCAGCAGAT GCTGGAGCAA CTGAAGAAGC AGCAGGAAGC CGCACAGGCG 
GCGGCAGCAA CAGCATCAGA ACATTCCAGG GAGCCCAGTG CAGCGGGCAG GCTCTCAGAT 
AGCTCTTCAG AAGCCTCCAA GTTGAGTTCA AAGAGCGCTA AAGAAAGGCG GAATCGGAGG 
AAAAAAAGAA AACAGAAAGA GCAGTCTGGA GGGGAAGAGA AAGATGATGA TGAATTCCAC 
AAATCCGAGT CCGAAGATAG CATCAGGAGG AAGGGCTTCC GCTTCTCCAT AGAAGGGAAC 
AGACTGACCT ATGAAAAGAG GTACTCTTCC CCACATCAGT CTCTGTTGAG CATTCGCGGC 
TCACTGTTTT CCCCGAGACG CAATAGCAGA ACAAGTCTTT TCAGCTTCAG AGGGCGAGCG 
AAGGACGTGG GGTCTGAGAA TGATTTTGCG GACGATGAGC ACAGCACCTT CGAGGACAAT 
GAGAGCAGGA GAGACTCTCT GTTTGTTCCC CGAAGACATG GAGAGCGACG CAACAGTAAC 
CTCAGCCAAA CCAGCAGATC CTCCCGAATG CTGGCGGGGC TTCCAGCAAA CGGGAAGATG 
CACAGCACAG TGGATTGCAA TGGTGTGGTT TCCTTGGTGG GCGGACCCTC GGTTCCGACA 
TCGCCAGTTG GACAGCTTCT GCCAGAGGTG ATAATAGATA AGCCAGCTAC TGATGACAAT 
GGAACAACTA CTGAAACTGA GATGAGAAAG AGGAGGTCAA GTTCTTTCCA TGTTTCCATG 
GACTTTCTTG AGGATCCTTC CCAGAGGCAA AGGGCAATGA GCATTGCCAG CATCTTAACA 
AATACAGTAG AAGAACTAGA AGAATCCAGA CAGAAATGTC CACCCTGTTG GTATAAATTT 
TCCAACATAT TTTTAATTTG GGACTGTTCT CCATATTGGC TAAAAGTTAA ACATATTGTC 
AACCTGGTTG TGATGGACCC ATTTGTTGAC CTGGCAATTA CTATCTGTAT TGTGTTAAAT 
ACCCTTTTCA TGGCCATGGA GCACTACCCC ATGACTGAAC ATTTCAATCA TGTACTTACA 
GTAGGAAACT TGGTTTTCAC TGGGATCTTC ACAGCAGAAA TGTTCCTGAA AATCATTGCC 
ATGGACCCTT ACTATTATTT CCAAGAGGGC TGGAATATCT TTGATGGTTT CATTGTGACA 
CTCAGCCTGG TAGAACTTGG CCTTGCCAAT GTGGAAGGGT TATCAGTTCT CCGTTCATTC 
CGACTGCTCC GAGTCTTCAA GTTGGCAAAG TCCTGGCCCA CACTGAACAT GCTCATTAAG 
ATCATCGGCA ACTCGGTGGG CGCACTGGGC AACCTGACCC TGGTGCTGGC CATCATCGTC 
TTCATTTTTG CCGTGGTCGG CATGCAGCTG TTCGGCAAAA GTTACAAAGA TTGTGTCTGC 
AAAATTGCCA CTGACTGCAA ACTCCCGCGC TGGCACATGA ACGACTTCTT CCACTCCTTC 
CTGATCGTGT TCCGAGTGCT GTGTGGGGAG TGGATAGAGA CCATGTGGGA CTGCATGGAG 
GTCGCGGGTC AAGCCATGTG CCTTACTGTC TTCATGATGG TCATGGTGAT TGGGAACCTT 
GTGGTCCTGA ACCTCTTTCT GGCCTTGCTT CTGAGCTCAT TTAGTGCAGA CAACCTTGCA 
GCCACTGACG ATGACAACGA AATGAACAAC CTTCAAATTG CTGTGGACAG GATGCACAAA 
GGAGTAGCTT ATGTAAAAAG AAAAATATAT GAGTTTATTC AACAGTCCTT TGTTAGGAAA 
CAGAAGATCC TAGATGAAAT TAAGCCACTT GATGATCTAA ACAACAGAAA AGACAATTGT 
ACATCTAACC ACACGACAGA GATTGGGAAA GATCTGGACT GTCTGAAAGA TGTGAATGGA 
ACCACGAGTG GCATAGGGAC CGGCAGCAGT GTGGAAAAGT ACATCATTGA TGAGAGTGAC 
TACATGTCAT TCATAAACAA CCCCAGCCTC ACCGTGACTG TGCCCATTGC TGTGGGAGAG 
TCTGACTTTG AAAACTTAAA CACAGAGGAC TTTAGCAGTG AATCAGATCT AGAAGAAAGC 
AAAGAGAAAC TCAACGAAAG CAGTAGTTCC TCAGAAGGAA GCACAGTAGA CATCGGGGCA 
CCTGCGGAGG AACAGCCTGT CATGGAACCA GAAGAAACCC TTGAGCCCGA AGCTTGCTTC 
ACTGAAGGCT GTGTGCAGAG ATTCAAGTGC TGTCAAATCA GTGTGGAAGA AGGAAGAGGA 
AAACAGTGGT GGAACCTTCG GAGAACGTGC TTCCGAATAG TTGAGCACAA CTGGTTTGAG 
ACCTTCATTG TGTTCATGAT TCTCCTCAGT AGTGGTGCCC TGGCCTTTGA GGATATATAC 
ATTGATCAGC GAAAGACGAT CAAGACCATG CTGGAGTATG CAGACAAGGT TTTCACTTAC 
ATTTTTATCC TGGAGATGCT CCTCAAATGG GTAGCCTACG GCTATCAAAC GTATTTCACC 
AATGCCTGGT GTTGGCTGGA CTTCTTAATT GTTGATGTTT CATTGGTCAG TTTAACAGCA 
AATGCCTTGG GTTACTCTGA ACTTGGGGCC ATCAAGTCCC TAAGGACACT AAGAGCTCTG 
AGACCCCTAA GAGCCTTATC ACGATTTGAA GGGATGAGGG TGGTTGTGAA TGCCCTGTTA 
GGAGCAATTC CATCCATCAT GAATGTGCTT CTGGTTTGCC TTATATTCTG GCTAATTTTC 
AGCATCATGG GCGTAAATTT GTTTGCTGGC AAATTCTACC ACTGTGTTAA CACCACAACT 
GGTGACACAT TTGAGATCAC CGAAGTCAAT AATCATTCTG ATTGCCTAAA ACTAATAGAA 
AGAAATGAGA CTGCCCGGTG GAAAAATGTG AAAGTAAACT TTGATAATGT AGGATTTGGG 
TATCTTTCTT TGCTTCAAGT TGCGACGTTT AAGGGTTGGA TGGATATCAT GTATGCAGCA 
GTTGATTCCA GAAATGTGGA ACTGCAGCCT AAGTATGAGG AAAGCCTGTA CATGTACCTG 
TACTTCGTCA TCTTCATCAT CTTCGGCTCG TTCTTCACTC TAAATCTATT CATCGGTGTC 
ATCATAGACA ACTTCAACCA GCAGAAGAAG AAGTTTGGAG GTCAAGACAT CTTTATGACA 
GAAGAACAGA AGAAATACTA TAATGCAATG AAGAAATTAG GATCAAAAAA GCCACAAAAG 
CCTATCCCTC GACCTGGAAA CAAATTTCAA GGAATGGTCT TTGACTTTGT AACCAGACAA 
GTGTTTGACA TCAGCATCAT GATCCTCATC TGTCTGAACA TGGTGACCAT GATGGTGGAA 
ACGGATGACC AGAGCGATTA TGTGACAAGC ATTTTGTCAC GTATCAACCT GGTGTTCATT 
GTGCTGTTCA CCGGCGAGTG CGTGCTGAAG CTCATCTCCC TCCGCCATTA TTATTTCACC 
ATTGGCTGGA ATATTTTCGA TTTTGTGGTG GTCATCCTCT CCATTGTAGG AATGTTTCTT 
GCGGAGCTGA TAGAGAAGTA TTTCGTGTCC CCTACCCTGT TCCGAGTCAT CCGCCTGGCC 
AGGATTGGAC GAATCCTACG CCTGATCAAA GGCGCCAAGG GGATCCGCAC TCTGCTCTTT 
GCTCTGATGA TGTCCCTTCC TGCGCTGTTC AACATCGGCC TCCTGCTTTT CCTGGTCATG 
TTCATCTACG CCATCTTTGG GATGTCCAAC TTTGCCTATG TTAAGAGGGA AGTTGGAATT 
GATGACATGT TCAACTTTGA GACTTTTGGC AACAGCATGA TCTGCCTGTT CCAAATCACC 
ACCTCTGCGG GCTGGGACGG ACTGCTGGCC CCCATCCTCA ACAGCAAACC CCCTGACTGT 
GACCCTAATA AAGTTAACCC CGGAAGCTCG GTGAAGGGGG ACTGTGGGAA CCCATCCGTG 
GGGATTTTCT TTTTTGTCAG CTACATCATC ATATCCTTCC TGGTGGTGGT GAACATGTAC 
ATCGCTGTCA TCCTGGAGAA CTTCAGCGTC GCCACCGAAG AAAGTGCAGA GCCCCTGAGT 
GAGGACGACT TTGAGATGTT CTACGAGGTC TGGGAGAAGT TCGACCCTGA CGCCACTCAG 
TTCATGGAAT TTGAAAAATT ATCTCAGTTT GCAGCTGCTC TCGAACCCCC TCTCAACTTA 
CCACAACCGA ACAAACTTCA GCTCATTGCC ATGGACCTGC CCATGGTGAG TGGAGACCGC 
ATCCACTGCC TGGACATCTT ATTTGCTTTT ACAAAGCGGG TCCTGGGCGA GAGTGGAGAG 
ATGGACGCTC TTCGAATCCA GATGGAAGAG CGCTTCATGG CTTCCAACCC CTCCAAGGTC 
TCCTATCAGC CAATCACTAC AACGTTAAAA CGAAAGCAAG AGGAGGTGTC AGCCGTTATC 
ATTCAGCGCG CTTACAGGCG CCACCTTTTG AAGCGAACAG TAAAACAAGC TTCATTCACA 
TACAATAAGA ACAAACTCAA GGGCGGGGCT AATCTTCTTG TAAAAGAAGA CATGATCATT 
GACAGGATAA ATGAAAACTC TATTACAGAG AAAACTGACC TGACGATGTC CACTGCAGCT 
TGTCCGCCCT CCTACGACCG GGTGACAAAG CCAATCGTGG AGAAACACGA GCAGGAAGGA 
AAAGATGAGA AAGCCAAAGG GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selective spider toxins reveal a role for the Nav1.1 channel in mechanical pain.
Nature534(7608)494-9(2016 06)
Osteen JD,Herzig V,Gilchrist J,Emrick JJ,Zhang C,Wang X,Castro J,Garcia-Caraballo S,Grundy L,Rychkov GY,Weyer AD,Dekan Z,Undheim EA,Alewood P,Stucky CL,Brierley SM,Basbaum AI,Bosmans F,King GF,Julius D

Variants of Transient Receptor Potential Melastatin Member 4 in Childhood Atrioventricular Block.
Journal of the American Heart Association5(5)(2016 May)
Syam N,Chatel S,Ozhathil LC,Sottas V,Rougier JS,Baruteau A,Baron E,Amarouch MY,Daumy X,Probst V,Schott JJ,Abriel H

Cognitive Deficits Associated with Nav1.1 Alterations: Involvement of Neuronal Firing Dynamics and Oscillations.
PloS one11(3)e0151538(2016)
Bender AC,Luikart BW,Lenck-Santini PP

Age-dependent alterations of voltage-gated Na(+) channel isoforms in rat sinoatrial node.
Mechanisms of ageing and development15280-90(2015 Dec)
Huang X,Du Y,Yang P,Lin S,Xi Y,Yang Z,Ma A

MicroRNA-9 induces defective trafficking of Nav1.1 and Nav1.2 by targeting Nav?2 protein coding region in rat with chronic brain hypoperfusion.
Molecular neurodegeneration1036(2015 Aug)
Sun LH,Yan ML,Hu XL,Peng LW,Che H,Bao YN,Guo F,Liu T,Chen X,Zhang R,Ban T,Wang N,Liu HL,Hou X,Ai J

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Scn1a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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