SCN1A cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SCN1A
Entrez Gene ID	6323
Full Name	sodium voltage-gated channel alpha subunit 1
Synonyms	EIEE6,FEB3,FEB3A,FHM3,GEFSP2,HBSCI,NAC1,Nav1.1,SCN1,SMEI
General protein information	Preferred Names sodium voltage-gated channel alpha subunit 1 Names sodium channel protein type 1 subunit alpha sodium channel protein type I subunit alpha sodium channel protein, brain I alpha subunit sodium channel voltage gated type 1 alpha subunit sodium channel, voltage-gated, type I, alpha polypeptide sodium channel, voltage-gated, type I, alpha subunit voltage-gated sodium channel subunit alpha Nav1.1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q24.3
Summary	Voltage-dependent sodium channels are heteromeric complexes that regulate sodium exchange between intracellular and extracellular spaces and are essential for the generation and propagation of action potentials in muscle cells and neurons. Each sodium channel is composed of a large pore-forming, glycosylated alpha subunit and two smaller beta subunits. This gene encodes a sodium channel alpha subunit, which has four homologous domains, each of which contains six transmembrane regions. Allelic variants of this gene are associated with generalized epilepsy with febrile seizures and epileptic encephalopathy. Alternative splicing results in multiple transcript variants. The RefSeq Project has decided to create four representative RefSeq records. Three of the transcript variants are supported by experimental evidence and the fourth contains alternate 5' untranslated exons, the exact combination of which have not been experimentally confirmed for the full-length transcript. [provided by RefSeq, Oct 2015].
Disorder MIM:	182389
Disorder Html:	Epilepsy, generalized, with febrile seizures plus, type 2, 604403 (3); Migraine, familial hemiplegic, 3, 609634 (3); Febrile seizures, familial, 3A, 604403 (3); Epileptic encephalopathy, early infantile, 6 (Dravet syndrome), 607208 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011511599.1	XP_011509901.1	sodium channel protein type 1 subunit alpha isoform X3
XM_017004644.1	XP_016860133.1	sodium channel protein type 1 subunit alpha isoform X1
XM_017004645.1	XP_016860134.1	sodium channel protein type 1 subunit alpha isoform X2
XM_017004646.1	XP_016860135.1	sodium channel protein type 1 subunit alpha isoform X2
XM_011511605.2	XP_011509907.1	sodium channel protein type 1 subunit alpha isoform X3
NM_001202435.1	NP_001189364.1	sodium channel protein type 1 subunit alpha isoform 1
NM_001353949.1	NP_001340878.1	sodium channel protein type 1 subunit alpha isoform 2
NM_001353957.1	NP_001340886.1	sodium channel protein type 1 subunit alpha isoform 3
NM_001353954.1	NP_001340883.1	sodium channel protein type 1 subunit alpha isoform 4
NM_001353955.1	NP_001340884.1	sodium channel protein type 1 subunit alpha isoform 4
NM_001353960.2	NP_001340889.1	sodium channel protein type 1 subunit alpha isoform 5
NM_001353948.2	NP_001340877.1	sodium channel protein type 1 subunit alpha isoform 1
NM_006920.6	NP_008851.3	sodium channel protein type 1 subunit alpha isoform 2
NM_001353954.2	NP_001340883.1	sodium channel protein type 1 subunit alpha isoform 4
NM_001353948.1	NP_001340877.1	sodium channel protein type 1 subunit alpha isoform 1
NM_006920.4	NP_008851.3	sodium channel protein type 1 subunit alpha isoform 2
XM_017004651.1	XP_016860140.1	sodium channel protein type 1 subunit alpha isoform X4
NM_001353952.1	NP_001340881.1	sodium channel protein type 1 subunit alpha isoform 2
NM_001165964.3	NP_001159436.1	sodium channel protein type 1 subunit alpha isoform 3
NM_001353955.2	NP_001340884.1	sodium channel protein type 1 subunit alpha isoform 4
XM_011511602.2	XP_011509904.1	sodium channel protein type 1 subunit alpha isoform X1
XM_017004648.1	XP_016860137.1	sodium channel protein type 1 subunit alpha isoform X2
NM_001165963.3	NP_001159435.1	sodium channel protein type 1 subunit alpha isoform 1
NM_001202435.3	NP_001189364.1	sodium channel protein type 1 subunit alpha isoform 1
NM_001353949.2	NP_001340878.1	sodium channel protein type 1 subunit alpha isoform 2
NM_001353952.2	NP_001340881.1	sodium channel protein type 1 subunit alpha isoform 2
NM_001165964.1	NP_001159436.1	sodium channel protein type 1 subunit alpha isoform 3
XM_011511598.1	XP_011509900.1	sodium channel protein type 1 subunit alpha isoform X3
XM_011511600.1	XP_011509902.1	sodium channel protein type 1 subunit alpha isoform X3
XM_011511601.1	XP_011509903.1	sodium channel protein type 1 subunit alpha isoform X3
XM_017004650.1	XP_016860139.1	sodium channel protein type 1 subunit alpha isoform X3
XM_011511606.2	XP_011509908.1	sodium channel protein type 1 subunit alpha isoform X4
XM_017004652.1	XP_016860141.1	sodium channel protein type 1 subunit alpha isoform X4
XM_017004653.1	XP_016860142.1	sodium channel protein type 1 subunit alpha isoform X5
XM_017004654.1	XP_016860143.1	sodium channel protein type 1 subunit alpha isoform X6
NM_001353951.1	NP_001340880.1	sodium channel protein type 1 subunit alpha isoform 2
NM_001353950.1	NP_001340879.1	sodium channel protein type 1 subunit alpha isoform 2
NM_001353961.1	NP_001340890.1	sodium channel protein type 1 subunit alpha isoform 6
NM_001202435.2	NP_001189364.1	sodium channel protein type 1 subunit alpha isoform 1
NM_001353958.2	NP_001340887.1	sodium channel protein type 1 subunit alpha isoform 3
NM_001353950.2	NP_001340879.1	sodium channel protein type 1 subunit alpha isoform 2
NM_001353961.2	NP_001340890.1	sodium channel protein type 1 subunit alpha isoform 6
NM_001353951.2	NP_001340880.1	sodium channel protein type 1 subunit alpha isoform 2
NM_001353960.1	NP_001340889.1	sodium channel protein type 1 subunit alpha isoform 5
NM_001353958.1	NP_001340887.1	sodium channel protein type 1 subunit alpha isoform 3
NM_006920.5	NP_008851.3	sodium channel protein type 1 subunit alpha isoform 2
NM_001165963.2	NP_001159435.1	sodium channel protein type 1 subunit alpha isoform 1
NM_001165964.2	NP_001159436.1	sodium channel protein type 1 subunit alpha isoform 3
XM_011511602.1	XP_011509904.1	sodium channel protein type 1 subunit alpha isoform X3
NM_001165963.1	NP_001159435.1	sodium channel protein type 1 subunit alpha isoform 1
XM_011511604.2	XP_011509906.1	sodium channel protein type 1 subunit alpha isoform X2
XM_017004647.1	XP_016860136.1	sodium channel protein type 1 subunit alpha isoform X2
XM_017004649.1	XP_016860138.1	sodium channel protein type 1 subunit alpha isoform X2
NM_001353957.2	NP_001340886.1	sodium channel protein type 1 subunit alpha isoform 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	SCN1A	NP_001159435.1
Mus musculus (house mouse)	Scn1a	NP_061203.2
Gallus gallus (chicken)	SCN1A	XP_003641634.1
	SCN1A	XP_001101023.1
Bos taurus (cattle)	SCN1A	NP_001180147.1
Pan troglodytes (chimpanzee)	SCN1A	XP_003309330.1
Canis lupus familiaris (dog)	SCN1A	XP_005640320.1
Rattus norvegicus (Norway rat)	Scn1a	NP_110502.1

Gene Ontology
Bibliography

Related articles in PubMed

The potential implication of SCN1A and CYP3A5 genetic variants on antiepileptic drug resistance among Egyptian epileptic children.
Abo El Fotoh WM, Abd El Naby SA, Habib MS, ALrefai AA, Kasemy ZA
Seizure4175-80(2016 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In this study, we performed exome sequencing in six patients with SCN1A-negative Dravet syndrome to identify other genes related to this disorder..the data in this study identify GABRB3 as a candidate gene for Dravet syndrome
Title: A mutation in GABRB3 associated with Dravet syndrome.

Pathogenic, likely pathogenic, and benign variants in SCNs were identified using databases of sodium channel variants. Benign variants were also identified from population-based databases. Eight algorithms commonly used to predict pathogenicity were compared. In addition, logistic regression was used to determine if a combination of algorithms could better predict pathogenicity.
Title: Comparison and optimization of in silico algorithms for predicting the pathogenicity of sodium channel variants in epilepsy.

This study demonstrated that early-life prolonged FSs have a profound long-term impact on neuronal function and adult seizure phenotypes in a mouse model of human SCN1A dysfunction.
Title: Early-life febrile seizures worsen adult phenotypes in Scn1a mutants.

A novel SCN1A mutation was found in an 8-year-old boy with GEFS+. The father has the same mutation, but he only had childhood simple febrile seizures.
Title: A novel inherited SCN1A mutation associated with GEFS+ in benign and encephalopathic epilepsy.

The frequency of pathogenic variants was 4.17% in the patients with refractory epilepsy and Dravet syndrome and 11.1% in DS patients. L1775P missense mutation was predicted to be damaging with a score of 100% by the PolyPhen-2 program.
Title: Determination of SCN1A genetic variants in Mexican patients with refractory epilepsy and Dravet syndrome.

The following SCN1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SCN1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu89762	NM_001353961.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu89762	XM_017004654.1 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu22576	NM_001165963.3 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu22576	NM_001202435.3 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu22576	NM_001353948.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu22576	XM_011511601.1 Latest version!	Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu22576	XM_011511600.1 Latest version!	Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu22576	XM_011511602.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu22576	XM_011511599.1 Latest version!	Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu22576	XM_011511598.1 Latest version!	Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu22576	XM_017004644.1 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu89761	NM_001353960.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu89761	XM_017004653.1 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu23006	NM_001353958.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu23006	NM_001353957.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu23006	NM_001165964.3 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu25755	NM_001353951.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu25755	NM_001353950.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu25755	NM_001353952.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu25755	NM_001353949.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu25755	NM_006920.6 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu60371	NM_001353955.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu60371	NM_001353954.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu23006	XM_011511606.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu23006	XM_017004651.1 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu23006	XM_017004652.1 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu25755	XM_017004648.1 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu25755	XM_017004649.1 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu25755	XM_017004647.1 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu25755	XM_011511604.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu25755	XM_017004646.1 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu25755	XM_017004645.1 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu60371	XM_011511605.2 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu60371	XM_017004650.1 Latest version!	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu60371	NM_001353954.1	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu22576	NM_001353948.1	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu25755	NM_006920.4	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu60371	NM_001353955.1	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu22576	NM_001202435.1	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu25755	NM_001353949.1	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu25755	NM_001353952.1	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu23006	NM_001165964.1	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu22576	NM_001202435.2	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu25755	NM_001353950.1	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu89762	NM_001353961.1	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$643.30 $919.00
OHu25755	NM_001353951.1	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu89761	NM_001353960.1	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu23006	NM_001353958.1	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu25755	NM_006920.5	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu22576	NM_001165963.2	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu23006	NM_001165964.2	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu22576	XM_011511602.1	Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu22576	NM_001165963.1	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24	$923.30 $1319.00
OHu23006	NM_001353957.1	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SCN1A ( NM_001353961.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340890.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 15, mRNA.

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CloneID	OHu89762
Clone ID Related Accession (Same CDS sequence)	XM_017004654.1 , NM_001353961.1 , NM_001353961.2
Accession Version	NM_001353961.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.117623.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTCTGA AAATTATTGC CATGGATCCT TACTATTATT TCCAAGAAGG CTGGAATATC 
TTTGACGGTT TTATTGTGAC GCTTAGCCTG GTAGAACTTG GACTCGCCAA TGTGGAAGGA 
TTATCTGTTC TCCGTTCATT TCGATTGCTG CGAGTTTTCA AGTTGGCAAA ATCTTGGCCA 
ACGTTAAATA TGCTAATAAA GATCATCGGC AATTCCGTGG GGGCTCTGGG AAATTTAACC 
CTCGTCTTGG CCATCATCGT CTTCATTTTT GCCGTGGTCG GCATGCAGCT CTTTGGTAAA 
AGCTACAAAG ATTGTGTCTG CAAGATCGCC AGTGATTGTC AACTCCCACG CTGGCACATG 
AATGACTTCT TCCACTCCTT CCTGATTGTG TTCCGCGTGC TGTGTGGGGA GTGGATAGAG 
ACCATGTGGG ACTGTATGGA GGTTGCTGGT CAAGCCATGT GCCTTACTGT CTTCATGATG 
GTCATGGTGA TTGGAAACCT AGTGGTCCTG AATCTCTTTC TGGCCTTGCT TCTGAGCTCA 
TTTAGTGCAG ACAACCTTGC AGCCACTGAT GATGATAATG AAATGAATAA TCTCCAAATT 
GCTGTGGATA GGATGCACAA AGGAGTAGCT TATGTGAAAA GAAAAATATA TGAATTTATT 
CAACAGTCCT TCATTAGGAA ACAAAAGATT TTAGATGAAA TTAAACCACT TGATGATCTA 
AACAACAAGA AAGACAGTTG TATGTCCAAT CATACAGCAG AAATTGGGAA AGATCTTGAC 
TATCTTAAAG ATGTAAATGG AACTACAAGT GGTATAGGAA CTGGCAGCAG TGTTGAAAAA 
TACATTATTG ATGAAAGTGA TTACATGTCA TTCATAAACA ACCCCAGTCT TACTGTGACT 
GTACCAATTG CTGTAGGAGA ATCTGACTTT GAAAATTTAA ACACGGAAGA CTTTAGTAGT 
GAATCGGATC TGGAAGAAAG CAAAGAGAAA CTGAATGAAA GCAGTAGCTC ATCAGAAGGT 
AGCACTGTGG ACATCGGCGC ACCTGTAGAA GAACAGCCCG TAGTGGAACC TGAAGAAACT 
CTTGAACCAG AAGCTTGTTT CACTGAAGGC TGTGTACAAA GATTCAAGTG TTGTCAAATC 
AATGTGGAAG AAGGCAGAGG AAAACAATGG TGGAACCTGA GAAGGACGTG TTTCCGAATA 
GTTGAACATA ACTGGTTTGA GACCTTCATT GTTTTCATGA TTCTCCTTAG TAGTGGTGCT 
CTGGCATTTG AAGATATATA TATTGATCAG CGAAAGACGA TTAAGACGAT GTTGGAATAT 
GCTGACAAGG TTTTCACTTA CATTTTCATT CTGGAAATGC TTCTAAAATG GGTGGCATAT 
GGCTATCAAA CATATTTCAC CAATGCCTGG TGTTGGCTGG ACTTCTTAAT TGTTGATGTT 
TCATTGGTCA GTTTAACAGC AAATGCCTTG GGTTACTCAG AACTTGGAGC CATCAAATCT 
CTCAGGACAC TAAGAGCTCT GAGACCTCTA AGAGCCTTAT CTCGATTTGA AGGGATGAGG 
GTGGTTGTGA ATGCCCTTTT AGGAGCAATT CCATCCATCA TGAATGTGCT TCTGGTTTGT 
CTTATATTCT GGCTAATTTT CAGCATCATG GGCGTAAATT TGTTTGCTGG CAAATTCTAC 
CACTGTATTA ACACCACAAC TGGTGACAGG TTTGACATCG AAGACGTGAA TAATCATACT 
GATTGCCTAA AACTAATAGA AAGAAATGAG ACTGCTCGAT GGAAAAATGT GAAAGTAAAC 
TTTGATAATG TAGGATTTGG GTATCTCTCT TTGCTTCAAG TTGCCACATT CAAAGGATGG 
ATGGATATAA TGTATGCAGC AGTTGATTCC AGAAATGTGG AACTCCAGCC TAAGTATGAA 
GAAAGTCTGT ACATGTATCT TTACTTTGTT ATTTTCATCA TCTTTGGGTC CTTCTTCACC 
TTGAACCTGT TTATTGGTGT CATCATAGAT AATTTCAACC AGCAGAAAAA GAAGTTTGGA 
GGTCAAGACA TCTTTATGAC AGAAGAACAG AAGAAATACT ATAATGCAAT GAAAAAATTA 
GGATCGAAAA AACCGCAAAA GCCTATACCT CGACCAGGAA ACAAATTTCA AGGAATGGTC 
TTTGACTTCG TAACCAGACA AGTTTTTGAC ATAAGCATCA TGATTCTCAT CTGTCTTAAC 
ATGGTCACAA TGATGGTGGA AACAGATGAC CAGAGTGAAT ATGTGACTAC CATTTTGTCA 
CGCATCAATC TGGTGTTCAT TGTGCTATTT ACTGGAGAGT GTGTACTGAA ACTCATCTCT 
CTACGCCATT ATTATTTTAC CATTGGATGG AATATTTTTG ATTTTGTGGT TGTCATTCTC 
TCCATTGTAG GTATGTTTCT TGCCGAGCTG ATAGAAAAGT ATTTCGTGTC CCCTACCCTG 
TTCCGAGTGA TCCGTCTTGC TAGGATTGGC CGAATCCTAC GTCTGATCAA AGGAGCAAAG 
GGGATCCGCA CGCTGCTCTT TGCTTTGATG ATGTCCCTTC CTGCGTTGTT TAACATCGGC 
CTCCTACTCT TCCTAGTCAT GTTCATCTAC GCCATCTTTG GGATGTCCAA CTTTGCCTAT 
GTTAAGAGGG AAGTTGGGAT CGATGACATG TTCAACTTTG AGACCTTTGG CAACAGCATG 
ATCTGCCTAT TCCAAATTAC AACCTCTGCT GGCTGGGATG GATTGCTAGC ACCCATTCTC 
AACAGTAAGC CACCCGACTG TGACCCTAAT AAAGTTAACC CTGGAAGCTC AGTTAAGGGA 
GACTGTGGGA ACCCATCTGT TGGAATTTTC TTTTTTGTCA GTTACATCAT CATATCCTTC 
CTGGTTGTGG TGAACATGTA CATCGCGGTC ATCCTGGAGA ACTTCAGTGT TGCTACTGAA 
GAAAGTGCAG AGCCTCTGAG TGAGGATGAC TTTGAGATGT TCTATGAGGT TTGGGAGAAG 
TTTGATCCCG ATGCAACTCA GTTCATGGAA TTTGAAAAAT TATCTCAGTT TGCAGCTGCG 
CTTGAACCGC CTCTCAATCT GCCACAACCA AACAAACTCC AGCTCATTGC CATGGATTTG 
CCCATGGTGA GTGGTGACCG GATCCACTGT CTTGATATCT TATTTGCTTT TACAAAGCGG 
GTTCTAGGAG AGAGTGGAGA GATGGATGCT CTACGAATAC AGATGGAAGA GCGATTCATG 
GCTTCCAATC CTTCCAAGGT CTCCTATCAG CCAATCACTA CTACTTTAAA ACGAAAACAA 
GAGGAAGTAT CTGCTGTCAT TATTCAGCGT GCTTACAGAC GCCACCTTTT AAAGCGAACT 
GTAAAACAAG CTTCCTTTAC GTACAATAAA AACAAAATCA AAGGTGGGGC TAATCTTCTT 
ATAAAAGAAG ACATGATAAT TGACAGAATA AATGAAAACT CTATTACAGA AAAAACTGAT 
CTGACCATGT CCACTGCAGC TTGTCCACCT TCCTATGACC GGGTGACAAA GCCAATTGTG 
GAAAAACATG AGCAAGAAGG CAAAGATGAA AAAGCCAAAG GGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340890.1
CDS	2836..6423
Translation	MFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353961.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353961.1

ORF Insert Sequence:

ATGTTTCTGA AAATTATTGC CATGGATCCT TACTATTATT TCCAAGAAGG CTGGAATATC 
TTTGACGGTT TTATTGTGAC GCTTAGCCTG GTAGAACTTG GACTCGCCAA TGTGGAAGGA 
TTATCTGTTC TCCGTTCATT TCGATTGCTG CGAGTTTTCA AGTTGGCAAA ATCTTGGCCA 
ACGTTAAATA TGCTAATAAA GATCATCGGC AATTCCGTGG GGGCTCTGGG AAATTTAACC 
CTCGTCTTGG CCATCATCGT CTTCATTTTT GCCGTGGTCG GCATGCAGCT CTTTGGTAAA 
AGCTACAAAG ATTGTGTCTG CAAGATCGCC AGTGATTGTC AACTCCCACG CTGGCACATG 
AATGACTTCT TCCACTCCTT CCTGATTGTG TTCCGCGTGC TGTGTGGGGA GTGGATAGAG 
ACCATGTGGG ACTGTATGGA GGTTGCTGGT CAAGCCATGT GCCTTACTGT CTTCATGATG 
GTCATGGTGA TTGGAAACCT AGTGGTCCTG AATCTCTTTC TGGCCTTGCT TCTGAGCTCA 
TTTAGTGCAG ACAACCTTGC AGCCACTGAT GATGATAATG AAATGAATAA TCTCCAAATT 
GCTGTGGATA GGATGCACAA AGGAGTAGCT TATGTGAAAA GAAAAATATA TGAATTTATT 
CAACAGTCCT TCATTAGGAA ACAAAAGATT TTAGATGAAA TTAAACCACT TGATGATCTA 
AACAACAAGA AAGACAGTTG TATGTCCAAT CATACAGCAG AAATTGGGAA AGATCTTGAC 
TATCTTAAAG ATGTAAATGG AACTACAAGT GGTATAGGAA CTGGCAGCAG TGTTGAAAAA 
TACATTATTG ATGAAAGTGA TTACATGTCA TTCATAAACA ACCCCAGTCT TACTGTGACT 
GTACCAATTG CTGTAGGAGA ATCTGACTTT GAAAATTTAA ACACGGAAGA CTTTAGTAGT 
GAATCGGATC TGGAAGAAAG CAAAGAGAAA CTGAATGAAA GCAGTAGCTC ATCAGAAGGT 
AGCACTGTGG ACATCGGCGC ACCTGTAGAA GAACAGCCCG TAGTGGAACC TGAAGAAACT 
CTTGAACCAG AAGCTTGTTT CACTGAAGGC TGTGTACAAA GATTCAAGTG TTGTCAAATC 
AATGTGGAAG AAGGCAGAGG AAAACAATGG TGGAACCTGA GAAGGACGTG TTTCCGAATA 
GTTGAACATA ACTGGTTTGA GACCTTCATT GTTTTCATGA TTCTCCTTAG TAGTGGTGCT 
CTGGCATTTG AAGATATATA TATTGATCAG CGAAAGACGA TTAAGACGAT GTTGGAATAT 
GCTGACAAGG TTTTCACTTA CATTTTCATT CTGGAAATGC TTCTAAAATG GGTGGCATAT 
GGCTATCAAA CATATTTCAC CAATGCCTGG TGTTGGCTGG ACTTCTTAAT TGTTGATGTT 
TCATTGGTCA GTTTAACAGC AAATGCCTTG GGTTACTCAG AACTTGGAGC CATCAAATCT 
CTCAGGACAC TAAGAGCTCT GAGACCTCTA AGAGCCTTAT CTCGATTTGA AGGGATGAGG 
GTGGTTGTGA ATGCCCTTTT AGGAGCAATT CCATCCATCA TGAATGTGCT TCTGGTTTGT 
CTTATATTCT GGCTAATTTT CAGCATCATG GGCGTAAATT TGTTTGCTGG CAAATTCTAC 
CACTGTATTA ACACCACAAC TGGTGACAGG TTTGACATCG AAGACGTGAA TAATCATACT 
GATTGCCTAA AACTAATAGA AAGAAATGAG ACTGCTCGAT GGAAAAATGT GAAAGTAAAC 
TTTGATAATG TAGGATTTGG GTATCTCTCT TTGCTTCAAG TTGCCACATT CAAAGGATGG 
ATGGATATAA TGTATGCAGC AGTTGATTCC AGAAATGTGG AACTCCAGCC TAAGTATGAA 
GAAAGTCTGT ACATGTATCT TTACTTTGTT ATTTTCATCA TCTTTGGGTC CTTCTTCACC 
TTGAACCTGT TTATTGGTGT CATCATAGAT AATTTCAACC AGCAGAAAAA GAAGTTTGGA 
GGTCAAGACA TCTTTATGAC AGAAGAACAG AAGAAATACT ATAATGCAAT GAAAAAATTA 
GGATCGAAAA AACCGCAAAA GCCTATACCT CGACCAGGAA ACAAATTTCA AGGAATGGTC 
TTTGACTTCG TAACCAGACA AGTTTTTGAC ATAAGCATCA TGATTCTCAT CTGTCTTAAC 
ATGGTCACAA TGATGGTGGA AACAGATGAC CAGAGTGAAT ATGTGACTAC CATTTTGTCA 
CGCATCAATC TGGTGTTCAT TGTGCTATTT ACTGGAGAGT GTGTACTGAA ACTCATCTCT 
CTACGCCATT ATTATTTTAC CATTGGATGG AATATTTTTG ATTTTGTGGT TGTCATTCTC 
TCCATTGTAG GTATGTTTCT TGCCGAGCTG ATAGAAAAGT ATTTCGTGTC CCCTACCCTG 
TTCCGAGTGA TCCGTCTTGC TAGGATTGGC CGAATCCTAC GTCTGATCAA AGGAGCAAAG 
GGGATCCGCA CGCTGCTCTT TGCTTTGATG ATGTCCCTTC CTGCGTTGTT TAACATCGGC 
CTCCTACTCT TCCTAGTCAT GTTCATCTAC GCCATCTTTG GGATGTCCAA CTTTGCCTAT 
GTTAAGAGGG AAGTTGGGAT CGATGACATG TTCAACTTTG AGACCTTTGG CAACAGCATG 
ATCTGCCTAT TCCAAATTAC AACCTCTGCT GGCTGGGATG GATTGCTAGC ACCCATTCTC 
AACAGTAAGC CACCCGACTG TGACCCTAAT AAAGTTAACC CTGGAAGCTC AGTTAAGGGA 
GACTGTGGGA ACCCATCTGT TGGAATTTTC TTTTTTGTCA GTTACATCAT CATATCCTTC 
CTGGTTGTGG TGAACATGTA CATCGCGGTC ATCCTGGAGA ACTTCAGTGT TGCTACTGAA 
GAAAGTGCAG AGCCTCTGAG TGAGGATGAC TTTGAGATGT TCTATGAGGT TTGGGAGAAG 
TTTGATCCCG ATGCAACTCA GTTCATGGAA TTTGAAAAAT TATCTCAGTT TGCAGCTGCG 
CTTGAACCGC CTCTCAATCT GCCACAACCA AACAAACTCC AGCTCATTGC CATGGATTTG 
CCCATGGTGA GTGGTGACCG GATCCACTGT CTTGATATCT TATTTGCTTT TACAAAGCGG 
GTTCTAGGAG AGAGTGGAGA GATGGATGCT CTACGAATAC AGATGGAAGA GCGATTCATG 
GCTTCCAATC CTTCCAAGGT CTCCTATCAG CCAATCACTA CTACTTTAAA ACGAAAACAA 
GAGGAAGTAT CTGCTGTCAT TATTCAGCGT GCTTACAGAC GCCACCTTTT AAAGCGAACT 
GTAAAACAAG CTTCCTTTAC GTACAATAAA AACAAAATCA AAGGTGGGGC TAATCTTCTT 
ATAAAAGAAG ACATGATAAT TGACAGAATA AATGAAAACT CTATTACAGA AAAAACTGAT 
CTGACCATGT CCACTGCAGC TTGTCCACCT TCCTATGACC GGGTGACAAA GCCAATTGTG 
GAAAAACATG AGCAAGAAGG CAAAGATGAA AAAGCCAAAG GGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353961.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340890.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 15, mRNA.

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CloneID	OHu89762
Clone ID Related Accession (Same CDS sequence)	XM_017004654.1 , NM_001353961.1 , NM_001353961.2
Accession Version	NM_001353961.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. On Jun 2, 2019 this sequence version replaced NM_001353961.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.117623.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTCTGA AAATTATTGC CATGGATCCT TACTATTATT TCCAAGAAGG CTGGAATATC 
TTTGACGGTT TTATTGTGAC GCTTAGCCTG GTAGAACTTG GACTCGCCAA TGTGGAAGGA 
TTATCTGTTC TCCGTTCATT TCGATTGCTG CGAGTTTTCA AGTTGGCAAA ATCTTGGCCA 
ACGTTAAATA TGCTAATAAA GATCATCGGC AATTCCGTGG GGGCTCTGGG AAATTTAACC 
CTCGTCTTGG CCATCATCGT CTTCATTTTT GCCGTGGTCG GCATGCAGCT CTTTGGTAAA 
AGCTACAAAG ATTGTGTCTG CAAGATCGCC AGTGATTGTC AACTCCCACG CTGGCACATG 
AATGACTTCT TCCACTCCTT CCTGATTGTG TTCCGCGTGC TGTGTGGGGA GTGGATAGAG 
ACCATGTGGG ACTGTATGGA GGTTGCTGGT CAAGCCATGT GCCTTACTGT CTTCATGATG 
GTCATGGTGA TTGGAAACCT AGTGGTCCTG AATCTCTTTC TGGCCTTGCT TCTGAGCTCA 
TTTAGTGCAG ACAACCTTGC AGCCACTGAT GATGATAATG AAATGAATAA TCTCCAAATT 
GCTGTGGATA GGATGCACAA AGGAGTAGCT TATGTGAAAA GAAAAATATA TGAATTTATT 
CAACAGTCCT TCATTAGGAA ACAAAAGATT TTAGATGAAA TTAAACCACT TGATGATCTA 
AACAACAAGA AAGACAGTTG TATGTCCAAT CATACAGCAG AAATTGGGAA AGATCTTGAC 
TATCTTAAAG ATGTAAATGG AACTACAAGT GGTATAGGAA CTGGCAGCAG TGTTGAAAAA 
TACATTATTG ATGAAAGTGA TTACATGTCA TTCATAAACA ACCCCAGTCT TACTGTGACT 
GTACCAATTG CTGTAGGAGA ATCTGACTTT GAAAATTTAA ACACGGAAGA CTTTAGTAGT 
GAATCGGATC TGGAAGAAAG CAAAGAGAAA CTGAATGAAA GCAGTAGCTC ATCAGAAGGT 
AGCACTGTGG ACATCGGCGC ACCTGTAGAA GAACAGCCCG TAGTGGAACC TGAAGAAACT 
CTTGAACCAG AAGCTTGTTT CACTGAAGGC TGTGTACAAA GATTCAAGTG TTGTCAAATC 
AATGTGGAAG AAGGCAGAGG AAAACAATGG TGGAACCTGA GAAGGACGTG TTTCCGAATA 
GTTGAACATA ACTGGTTTGA GACCTTCATT GTTTTCATGA TTCTCCTTAG TAGTGGTGCT 
CTGGCATTTG AAGATATATA TATTGATCAG CGAAAGACGA TTAAGACGAT GTTGGAATAT 
GCTGACAAGG TTTTCACTTA CATTTTCATT CTGGAAATGC TTCTAAAATG GGTGGCATAT 
GGCTATCAAA CATATTTCAC CAATGCCTGG TGTTGGCTGG ACTTCTTAAT TGTTGATGTT 
TCATTGGTCA GTTTAACAGC AAATGCCTTG GGTTACTCAG AACTTGGAGC CATCAAATCT 
CTCAGGACAC TAAGAGCTCT GAGACCTCTA AGAGCCTTAT CTCGATTTGA AGGGATGAGG 
GTGGTTGTGA ATGCCCTTTT AGGAGCAATT CCATCCATCA TGAATGTGCT TCTGGTTTGT 
CTTATATTCT GGCTAATTTT CAGCATCATG GGCGTAAATT TGTTTGCTGG CAAATTCTAC 
CACTGTATTA ACACCACAAC TGGTGACAGG TTTGACATCG AAGACGTGAA TAATCATACT 
GATTGCCTAA AACTAATAGA AAGAAATGAG ACTGCTCGAT GGAAAAATGT GAAAGTAAAC 
TTTGATAATG TAGGATTTGG GTATCTCTCT TTGCTTCAAG TTGCCACATT CAAAGGATGG 
ATGGATATAA TGTATGCAGC AGTTGATTCC AGAAATGTGG AACTCCAGCC TAAGTATGAA 
GAAAGTCTGT ACATGTATCT TTACTTTGTT ATTTTCATCA TCTTTGGGTC CTTCTTCACC 
TTGAACCTGT TTATTGGTGT CATCATAGAT AATTTCAACC AGCAGAAAAA GAAGTTTGGA 
GGTCAAGACA TCTTTATGAC AGAAGAACAG AAGAAATACT ATAATGCAAT GAAAAAATTA 
GGATCGAAAA AACCGCAAAA GCCTATACCT CGACCAGGAA ACAAATTTCA AGGAATGGTC 
TTTGACTTCG TAACCAGACA AGTTTTTGAC ATAAGCATCA TGATTCTCAT CTGTCTTAAC 
ATGGTCACAA TGATGGTGGA AACAGATGAC CAGAGTGAAT ATGTGACTAC CATTTTGTCA 
CGCATCAATC TGGTGTTCAT TGTGCTATTT ACTGGAGAGT GTGTACTGAA ACTCATCTCT 
CTACGCCATT ATTATTTTAC CATTGGATGG AATATTTTTG ATTTTGTGGT TGTCATTCTC 
TCCATTGTAG GTATGTTTCT TGCCGAGCTG ATAGAAAAGT ATTTCGTGTC CCCTACCCTG 
TTCCGAGTGA TCCGTCTTGC TAGGATTGGC CGAATCCTAC GTCTGATCAA AGGAGCAAAG 
GGGATCCGCA CGCTGCTCTT TGCTTTGATG ATGTCCCTTC CTGCGTTGTT TAACATCGGC 
CTCCTACTCT TCCTAGTCAT GTTCATCTAC GCCATCTTTG GGATGTCCAA CTTTGCCTAT 
GTTAAGAGGG AAGTTGGGAT CGATGACATG TTCAACTTTG AGACCTTTGG CAACAGCATG 
ATCTGCCTAT TCCAAATTAC AACCTCTGCT GGCTGGGATG GATTGCTAGC ACCCATTCTC 
AACAGTAAGC CACCCGACTG TGACCCTAAT AAAGTTAACC CTGGAAGCTC AGTTAAGGGA 
GACTGTGGGA ACCCATCTGT TGGAATTTTC TTTTTTGTCA GTTACATCAT CATATCCTTC 
CTGGTTGTGG TGAACATGTA CATCGCGGTC ATCCTGGAGA ACTTCAGTGT TGCTACTGAA 
GAAAGTGCAG AGCCTCTGAG TGAGGATGAC TTTGAGATGT TCTATGAGGT TTGGGAGAAG 
TTTGATCCCG ATGCAACTCA GTTCATGGAA TTTGAAAAAT TATCTCAGTT TGCAGCTGCG 
CTTGAACCGC CTCTCAATCT GCCACAACCA AACAAACTCC AGCTCATTGC CATGGATTTG 
CCCATGGTGA GTGGTGACCG GATCCACTGT CTTGATATCT TATTTGCTTT TACAAAGCGG 
GTTCTAGGAG AGAGTGGAGA GATGGATGCT CTACGAATAC AGATGGAAGA GCGATTCATG 
GCTTCCAATC CTTCCAAGGT CTCCTATCAG CCAATCACTA CTACTTTAAA ACGAAAACAA 
GAGGAAGTAT CTGCTGTCAT TATTCAGCGT GCTTACAGAC GCCACCTTTT AAAGCGAACT 
GTAAAACAAG CTTCCTTTAC GTACAATAAA AACAAAATCA AAGGTGGGGC TAATCTTCTT 
ATAAAAGAAG ACATGATAAT TGACAGAATA AATGAAAACT CTATTACAGA AAAAACTGAT 
CTGACCATGT CCACTGCAGC TTGTCCACCT TCCTATGACC GGGTGACAAA GCCAATTGTG 
GAAAAACATG AGCAAGAAGG CAAAGATGAA AAAGCCAAAG GGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340890.1
CDS	2817..6404
Translation	MFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353961.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353961.2

ORF Insert Sequence:

ATGTTTCTGA AAATTATTGC CATGGATCCT TACTATTATT TCCAAGAAGG CTGGAATATC 
TTTGACGGTT TTATTGTGAC GCTTAGCCTG GTAGAACTTG GACTCGCCAA TGTGGAAGGA 
TTATCTGTTC TCCGTTCATT TCGATTGCTG CGAGTTTTCA AGTTGGCAAA ATCTTGGCCA 
ACGTTAAATA TGCTAATAAA GATCATCGGC AATTCCGTGG GGGCTCTGGG AAATTTAACC 
CTCGTCTTGG CCATCATCGT CTTCATTTTT GCCGTGGTCG GCATGCAGCT CTTTGGTAAA 
AGCTACAAAG ATTGTGTCTG CAAGATCGCC AGTGATTGTC AACTCCCACG CTGGCACATG 
AATGACTTCT TCCACTCCTT CCTGATTGTG TTCCGCGTGC TGTGTGGGGA GTGGATAGAG 
ACCATGTGGG ACTGTATGGA GGTTGCTGGT CAAGCCATGT GCCTTACTGT CTTCATGATG 
GTCATGGTGA TTGGAAACCT AGTGGTCCTG AATCTCTTTC TGGCCTTGCT TCTGAGCTCA 
TTTAGTGCAG ACAACCTTGC AGCCACTGAT GATGATAATG AAATGAATAA TCTCCAAATT 
GCTGTGGATA GGATGCACAA AGGAGTAGCT TATGTGAAAA GAAAAATATA TGAATTTATT 
CAACAGTCCT TCATTAGGAA ACAAAAGATT TTAGATGAAA TTAAACCACT TGATGATCTA 
AACAACAAGA AAGACAGTTG TATGTCCAAT CATACAGCAG AAATTGGGAA AGATCTTGAC 
TATCTTAAAG ATGTAAATGG AACTACAAGT GGTATAGGAA CTGGCAGCAG TGTTGAAAAA 
TACATTATTG ATGAAAGTGA TTACATGTCA TTCATAAACA ACCCCAGTCT TACTGTGACT 
GTACCAATTG CTGTAGGAGA ATCTGACTTT GAAAATTTAA ACACGGAAGA CTTTAGTAGT 
GAATCGGATC TGGAAGAAAG CAAAGAGAAA CTGAATGAAA GCAGTAGCTC ATCAGAAGGT 
AGCACTGTGG ACATCGGCGC ACCTGTAGAA GAACAGCCCG TAGTGGAACC TGAAGAAACT 
CTTGAACCAG AAGCTTGTTT CACTGAAGGC TGTGTACAAA GATTCAAGTG TTGTCAAATC 
AATGTGGAAG AAGGCAGAGG AAAACAATGG TGGAACCTGA GAAGGACGTG TTTCCGAATA 
GTTGAACATA ACTGGTTTGA GACCTTCATT GTTTTCATGA TTCTCCTTAG TAGTGGTGCT 
CTGGCATTTG AAGATATATA TATTGATCAG CGAAAGACGA TTAAGACGAT GTTGGAATAT 
GCTGACAAGG TTTTCACTTA CATTTTCATT CTGGAAATGC TTCTAAAATG GGTGGCATAT 
GGCTATCAAA CATATTTCAC CAATGCCTGG TGTTGGCTGG ACTTCTTAAT TGTTGATGTT 
TCATTGGTCA GTTTAACAGC AAATGCCTTG GGTTACTCAG AACTTGGAGC CATCAAATCT 
CTCAGGACAC TAAGAGCTCT GAGACCTCTA AGAGCCTTAT CTCGATTTGA AGGGATGAGG 
GTGGTTGTGA ATGCCCTTTT AGGAGCAATT CCATCCATCA TGAATGTGCT TCTGGTTTGT 
CTTATATTCT GGCTAATTTT CAGCATCATG GGCGTAAATT TGTTTGCTGG CAAATTCTAC 
CACTGTATTA ACACCACAAC TGGTGACAGG TTTGACATCG AAGACGTGAA TAATCATACT 
GATTGCCTAA AACTAATAGA AAGAAATGAG ACTGCTCGAT GGAAAAATGT GAAAGTAAAC 
TTTGATAATG TAGGATTTGG GTATCTCTCT TTGCTTCAAG TTGCCACATT CAAAGGATGG 
ATGGATATAA TGTATGCAGC AGTTGATTCC AGAAATGTGG AACTCCAGCC TAAGTATGAA 
GAAAGTCTGT ACATGTATCT TTACTTTGTT ATTTTCATCA TCTTTGGGTC CTTCTTCACC 
TTGAACCTGT TTATTGGTGT CATCATAGAT AATTTCAACC AGCAGAAAAA GAAGTTTGGA 
GGTCAAGACA TCTTTATGAC AGAAGAACAG AAGAAATACT ATAATGCAAT GAAAAAATTA 
GGATCGAAAA AACCGCAAAA GCCTATACCT CGACCAGGAA ACAAATTTCA AGGAATGGTC 
TTTGACTTCG TAACCAGACA AGTTTTTGAC ATAAGCATCA TGATTCTCAT CTGTCTTAAC 
ATGGTCACAA TGATGGTGGA AACAGATGAC CAGAGTGAAT ATGTGACTAC CATTTTGTCA 
CGCATCAATC TGGTGTTCAT TGTGCTATTT ACTGGAGAGT GTGTACTGAA ACTCATCTCT 
CTACGCCATT ATTATTTTAC CATTGGATGG AATATTTTTG ATTTTGTGGT TGTCATTCTC 
TCCATTGTAG GTATGTTTCT TGCCGAGCTG ATAGAAAAGT ATTTCGTGTC CCCTACCCTG 
TTCCGAGTGA TCCGTCTTGC TAGGATTGGC CGAATCCTAC GTCTGATCAA AGGAGCAAAG 
GGGATCCGCA CGCTGCTCTT TGCTTTGATG ATGTCCCTTC CTGCGTTGTT TAACATCGGC 
CTCCTACTCT TCCTAGTCAT GTTCATCTAC GCCATCTTTG GGATGTCCAA CTTTGCCTAT 
GTTAAGAGGG AAGTTGGGAT CGATGACATG TTCAACTTTG AGACCTTTGG CAACAGCATG 
ATCTGCCTAT TCCAAATTAC AACCTCTGCT GGCTGGGATG GATTGCTAGC ACCCATTCTC 
AACAGTAAGC CACCCGACTG TGACCCTAAT AAAGTTAACC CTGGAAGCTC AGTTAAGGGA 
GACTGTGGGA ACCCATCTGT TGGAATTTTC TTTTTTGTCA GTTACATCAT CATATCCTTC 
CTGGTTGTGG TGAACATGTA CATCGCGGTC ATCCTGGAGA ACTTCAGTGT TGCTACTGAA 
GAAAGTGCAG AGCCTCTGAG TGAGGATGAC TTTGAGATGT TCTATGAGGT TTGGGAGAAG 
TTTGATCCCG ATGCAACTCA GTTCATGGAA TTTGAAAAAT TATCTCAGTT TGCAGCTGCG 
CTTGAACCGC CTCTCAATCT GCCACAACCA AACAAACTCC AGCTCATTGC CATGGATTTG 
CCCATGGTGA GTGGTGACCG GATCCACTGT CTTGATATCT TATTTGCTTT TACAAAGCGG 
GTTCTAGGAG AGAGTGGAGA GATGGATGCT CTACGAATAC AGATGGAAGA GCGATTCATG 
GCTTCCAATC CTTCCAAGGT CTCCTATCAG CCAATCACTA CTACTTTAAA ACGAAAACAA 
GAGGAAGTAT CTGCTGTCAT TATTCAGCGT GCTTACAGAC GCCACCTTTT AAAGCGAACT 
GTAAAACAAG CTTCCTTTAC GTACAATAAA AACAAAATCA AAGGTGGGGC TAATCTTCTT 
ATAAAAGAAG ACATGATAAT TGACAGAATA AATGAAAACT CTATTACAGA AAAAACTGAT 
CTGACCATGT CCACTGCAGC TTGTCCACCT TCCTATGACC GGGTGACAAA GCCAATTGTG 
GAAAAACATG AGCAAGAAGG CAAAGATGAA AAAGCCAAAG GGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_017004654.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860143.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X18, mRNA.

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CloneID	OHu89762
Clone ID Related Accession (Same CDS sequence)	XM_017004654.1 , NM_001353961.1 , NM_001353961.2
Accession Version	XM_017004654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3588bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTCTGA AAATTATTGC CATGGATCCT TACTATTATT TCCAAGAAGG CTGGAATATC 
TTTGACGGTT TTATTGTGAC GCTTAGCCTG GTAGAACTTG GACTCGCCAA TGTGGAAGGA 
TTATCTGTTC TCCGTTCATT TCGATTGCTG CGAGTTTTCA AGTTGGCAAA ATCTTGGCCA 
ACGTTAAATA TGCTAATAAA GATCATCGGC AATTCCGTGG GGGCTCTGGG AAATTTAACC 
CTCGTCTTGG CCATCATCGT CTTCATTTTT GCCGTGGTCG GCATGCAGCT CTTTGGTAAA 
AGCTACAAAG ATTGTGTCTG CAAGATCGCC AGTGATTGTC AACTCCCACG CTGGCACATG 
AATGACTTCT TCCACTCCTT CCTGATTGTG TTCCGCGTGC TGTGTGGGGA GTGGATAGAG 
ACCATGTGGG ACTGTATGGA GGTTGCTGGT CAAGCCATGT GCCTTACTGT CTTCATGATG 
GTCATGGTGA TTGGAAACCT AGTGGTCCTG AATCTCTTTC TGGCCTTGCT TCTGAGCTCA 
TTTAGTGCAG ACAACCTTGC AGCCACTGAT GATGATAATG AAATGAATAA TCTCCAAATT 
GCTGTGGATA GGATGCACAA AGGAGTAGCT TATGTGAAAA GAAAAATATA TGAATTTATT 
CAACAGTCCT TCATTAGGAA ACAAAAGATT TTAGATGAAA TTAAACCACT TGATGATCTA 
AACAACAAGA AAGACAGTTG TATGTCCAAT CATACAGCAG AAATTGGGAA AGATCTTGAC 
TATCTTAAAG ATGTAAATGG AACTACAAGT GGTATAGGAA CTGGCAGCAG TGTTGAAAAA 
TACATTATTG ATGAAAGTGA TTACATGTCA TTCATAAACA ACCCCAGTCT TACTGTGACT 
GTACCAATTG CTGTAGGAGA ATCTGACTTT GAAAATTTAA ACACGGAAGA CTTTAGTAGT 
GAATCGGATC TGGAAGAAAG CAAAGAGAAA CTGAATGAAA GCAGTAGCTC ATCAGAAGGT 
AGCACTGTGG ACATCGGCGC ACCTGTAGAA GAACAGCCCG TAGTGGAACC TGAAGAAACT 
CTTGAACCAG AAGCTTGTTT CACTGAAGGC TGTGTACAAA GATTCAAGTG TTGTCAAATC 
AATGTGGAAG AAGGCAGAGG AAAACAATGG TGGAACCTGA GAAGGACGTG TTTCCGAATA 
GTTGAACATA ACTGGTTTGA GACCTTCATT GTTTTCATGA TTCTCCTTAG TAGTGGTGCT 
CTGGCATTTG AAGATATATA TATTGATCAG CGAAAGACGA TTAAGACGAT GTTGGAATAT 
GCTGACAAGG TTTTCACTTA CATTTTCATT CTGGAAATGC TTCTAAAATG GGTGGCATAT 
GGCTATCAAA CATATTTCAC CAATGCCTGG TGTTGGCTGG ACTTCTTAAT TGTTGATGTT 
TCATTGGTCA GTTTAACAGC AAATGCCTTG GGTTACTCAG AACTTGGAGC CATCAAATCT 
CTCAGGACAC TAAGAGCTCT GAGACCTCTA AGAGCCTTAT CTCGATTTGA AGGGATGAGG 
GTGGTTGTGA ATGCCCTTTT AGGAGCAATT CCATCCATCA TGAATGTGCT TCTGGTTTGT 
CTTATATTCT GGCTAATTTT CAGCATCATG GGCGTAAATT TGTTTGCTGG CAAATTCTAC 
CACTGTATTA ACACCACAAC TGGTGACAGG TTTGACATCG AAGACGTGAA TAATCATACT 
GATTGCCTAA AACTAATAGA AAGAAATGAG ACTGCTCGAT GGAAAAATGT GAAAGTAAAC 
TTTGATAATG TAGGATTTGG GTATCTCTCT TTGCTTCAAG TTGCCACATT CAAAGGATGG 
ATGGATATAA TGTATGCAGC AGTTGATTCC AGAAATGTGG AACTCCAGCC TAAGTATGAA 
GAAAGTCTGT ACATGTATCT TTACTTTGTT ATTTTCATCA TCTTTGGGTC CTTCTTCACC 
TTGAACCTGT TTATTGGTGT CATCATAGAT AATTTCAACC AGCAGAAAAA GAAGTTTGGA 
GGTCAAGACA TCTTTATGAC AGAAGAACAG AAGAAATACT ATAATGCAAT GAAAAAATTA 
GGATCGAAAA AACCGCAAAA GCCTATACCT CGACCAGGAA ACAAATTTCA AGGAATGGTC 
TTTGACTTCG TAACCAGACA AGTTTTTGAC ATAAGCATCA TGATTCTCAT CTGTCTTAAC 
ATGGTCACAA TGATGGTGGA AACAGATGAC CAGAGTGAAT ATGTGACTAC CATTTTGTCA 
CGCATCAATC TGGTGTTCAT TGTGCTATTT ACTGGAGAGT GTGTACTGAA ACTCATCTCT 
CTACGCCATT ATTATTTTAC CATTGGATGG AATATTTTTG ATTTTGTGGT TGTCATTCTC 
TCCATTGTAG GTATGTTTCT TGCCGAGCTG ATAGAAAAGT ATTTCGTGTC CCCTACCCTG 
TTCCGAGTGA TCCGTCTTGC TAGGATTGGC CGAATCCTAC GTCTGATCAA AGGAGCAAAG 
GGGATCCGCA CGCTGCTCTT TGCTTTGATG ATGTCCCTTC CTGCGTTGTT TAACATCGGC 
CTCCTACTCT TCCTAGTCAT GTTCATCTAC GCCATCTTTG GGATGTCCAA CTTTGCCTAT 
GTTAAGAGGG AAGTTGGGAT CGATGACATG TTCAACTTTG AGACCTTTGG CAACAGCATG 
ATCTGCCTAT TCCAAATTAC AACCTCTGCT GGCTGGGATG GATTGCTAGC ACCCATTCTC 
AACAGTAAGC CACCCGACTG TGACCCTAAT AAAGTTAACC CTGGAAGCTC AGTTAAGGGA 
GACTGTGGGA ACCCATCTGT TGGAATTTTC TTTTTTGTCA GTTACATCAT CATATCCTTC 
CTGGTTGTGG TGAACATGTA CATCGCGGTC ATCCTGGAGA ACTTCAGTGT TGCTACTGAA 
GAAAGTGCAG AGCCTCTGAG TGAGGATGAC TTTGAGATGT TCTATGAGGT TTGGGAGAAG 
TTTGATCCCG ATGCAACTCA GTTCATGGAA TTTGAAAAAT TATCTCAGTT TGCAGCTGCG 
CTTGAACCGC CTCTCAATCT GCCACAACCA AACAAACTCC AGCTCATTGC CATGGATTTG 
CCCATGGTGA GTGGTGACCG GATCCACTGT CTTGATATCT TATTTGCTTT TACAAAGCGG 
GTTCTAGGAG AGAGTGGAGA GATGGATGCT CTACGAATAC AGATGGAAGA GCGATTCATG 
GCTTCCAATC CTTCCAAGGT CTCCTATCAG CCAATCACTA CTACTTTAAA ACGAAAACAA 
GAGGAAGTAT CTGCTGTCAT TATTCAGCGT GCTTACAGAC GCCACCTTTT AAAGCGAACT 
GTAAAACAAG CTTCCTTTAC GTACAATAAA AACAAAATCA AAGGTGGGGC TAATCTTCTT 
ATAAAAGAAG ACATGATAAT TGACAGAATA AATGAAAACT CTATTACAGA AAAAACTGAT 
CTGACCATGT CCACTGCAGC TTGTCCACCT TCCTATGACC GGGTGACAAA GCCAATTGTG 
GAAAAACATG AGCAAGAAGG CAAAGATGAA AAAGCCAAAG GGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860143.1
CDS	2836..6423
Translation	MFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017004654.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004654.1

ORF Insert Sequence:

ATGTTTCTGA AAATTATTGC CATGGATCCT TACTATTATT TCCAAGAAGG CTGGAATATC 
TTTGACGGTT TTATTGTGAC GCTTAGCCTG GTAGAACTTG GACTCGCCAA TGTGGAAGGA 
TTATCTGTTC TCCGTTCATT TCGATTGCTG CGAGTTTTCA AGTTGGCAAA ATCTTGGCCA 
ACGTTAAATA TGCTAATAAA GATCATCGGC AATTCCGTGG GGGCTCTGGG AAATTTAACC 
CTCGTCTTGG CCATCATCGT CTTCATTTTT GCCGTGGTCG GCATGCAGCT CTTTGGTAAA 
AGCTACAAAG ATTGTGTCTG CAAGATCGCC AGTGATTGTC AACTCCCACG CTGGCACATG 
AATGACTTCT TCCACTCCTT CCTGATTGTG TTCCGCGTGC TGTGTGGGGA GTGGATAGAG 
ACCATGTGGG ACTGTATGGA GGTTGCTGGT CAAGCCATGT GCCTTACTGT CTTCATGATG 
GTCATGGTGA TTGGAAACCT AGTGGTCCTG AATCTCTTTC TGGCCTTGCT TCTGAGCTCA 
TTTAGTGCAG ACAACCTTGC AGCCACTGAT GATGATAATG AAATGAATAA TCTCCAAATT 
GCTGTGGATA GGATGCACAA AGGAGTAGCT TATGTGAAAA GAAAAATATA TGAATTTATT 
CAACAGTCCT TCATTAGGAA ACAAAAGATT TTAGATGAAA TTAAACCACT TGATGATCTA 
AACAACAAGA AAGACAGTTG TATGTCCAAT CATACAGCAG AAATTGGGAA AGATCTTGAC 
TATCTTAAAG ATGTAAATGG AACTACAAGT GGTATAGGAA CTGGCAGCAG TGTTGAAAAA 
TACATTATTG ATGAAAGTGA TTACATGTCA TTCATAAACA ACCCCAGTCT TACTGTGACT 
GTACCAATTG CTGTAGGAGA ATCTGACTTT GAAAATTTAA ACACGGAAGA CTTTAGTAGT 
GAATCGGATC TGGAAGAAAG CAAAGAGAAA CTGAATGAAA GCAGTAGCTC ATCAGAAGGT 
AGCACTGTGG ACATCGGCGC ACCTGTAGAA GAACAGCCCG TAGTGGAACC TGAAGAAACT 
CTTGAACCAG AAGCTTGTTT CACTGAAGGC TGTGTACAAA GATTCAAGTG TTGTCAAATC 
AATGTGGAAG AAGGCAGAGG AAAACAATGG TGGAACCTGA GAAGGACGTG TTTCCGAATA 
GTTGAACATA ACTGGTTTGA GACCTTCATT GTTTTCATGA TTCTCCTTAG TAGTGGTGCT 
CTGGCATTTG AAGATATATA TATTGATCAG CGAAAGACGA TTAAGACGAT GTTGGAATAT 
GCTGACAAGG TTTTCACTTA CATTTTCATT CTGGAAATGC TTCTAAAATG GGTGGCATAT 
GGCTATCAAA CATATTTCAC CAATGCCTGG TGTTGGCTGG ACTTCTTAAT TGTTGATGTT 
TCATTGGTCA GTTTAACAGC AAATGCCTTG GGTTACTCAG AACTTGGAGC CATCAAATCT 
CTCAGGACAC TAAGAGCTCT GAGACCTCTA AGAGCCTTAT CTCGATTTGA AGGGATGAGG 
GTGGTTGTGA ATGCCCTTTT AGGAGCAATT CCATCCATCA TGAATGTGCT TCTGGTTTGT 
CTTATATTCT GGCTAATTTT CAGCATCATG GGCGTAAATT TGTTTGCTGG CAAATTCTAC 
CACTGTATTA ACACCACAAC TGGTGACAGG TTTGACATCG AAGACGTGAA TAATCATACT 
GATTGCCTAA AACTAATAGA AAGAAATGAG ACTGCTCGAT GGAAAAATGT GAAAGTAAAC 
TTTGATAATG TAGGATTTGG GTATCTCTCT TTGCTTCAAG TTGCCACATT CAAAGGATGG 
ATGGATATAA TGTATGCAGC AGTTGATTCC AGAAATGTGG AACTCCAGCC TAAGTATGAA 
GAAAGTCTGT ACATGTATCT TTACTTTGTT ATTTTCATCA TCTTTGGGTC CTTCTTCACC 
TTGAACCTGT TTATTGGTGT CATCATAGAT AATTTCAACC AGCAGAAAAA GAAGTTTGGA 
GGTCAAGACA TCTTTATGAC AGAAGAACAG AAGAAATACT ATAATGCAAT GAAAAAATTA 
GGATCGAAAA AACCGCAAAA GCCTATACCT CGACCAGGAA ACAAATTTCA AGGAATGGTC 
TTTGACTTCG TAACCAGACA AGTTTTTGAC ATAAGCATCA TGATTCTCAT CTGTCTTAAC 
ATGGTCACAA TGATGGTGGA AACAGATGAC CAGAGTGAAT ATGTGACTAC CATTTTGTCA 
CGCATCAATC TGGTGTTCAT TGTGCTATTT ACTGGAGAGT GTGTACTGAA ACTCATCTCT 
CTACGCCATT ATTATTTTAC CATTGGATGG AATATTTTTG ATTTTGTGGT TGTCATTCTC 
TCCATTGTAG GTATGTTTCT TGCCGAGCTG ATAGAAAAGT ATTTCGTGTC CCCTACCCTG 
TTCCGAGTGA TCCGTCTTGC TAGGATTGGC CGAATCCTAC GTCTGATCAA AGGAGCAAAG 
GGGATCCGCA CGCTGCTCTT TGCTTTGATG ATGTCCCTTC CTGCGTTGTT TAACATCGGC 
CTCCTACTCT TCCTAGTCAT GTTCATCTAC GCCATCTTTG GGATGTCCAA CTTTGCCTAT 
GTTAAGAGGG AAGTTGGGAT CGATGACATG TTCAACTTTG AGACCTTTGG CAACAGCATG 
ATCTGCCTAT TCCAAATTAC AACCTCTGCT GGCTGGGATG GATTGCTAGC ACCCATTCTC 
AACAGTAAGC CACCCGACTG TGACCCTAAT AAAGTTAACC CTGGAAGCTC AGTTAAGGGA 
GACTGTGGGA ACCCATCTGT TGGAATTTTC TTTTTTGTCA GTTACATCAT CATATCCTTC 
CTGGTTGTGG TGAACATGTA CATCGCGGTC ATCCTGGAGA ACTTCAGTGT TGCTACTGAA 
GAAAGTGCAG AGCCTCTGAG TGAGGATGAC TTTGAGATGT TCTATGAGGT TTGGGAGAAG 
TTTGATCCCG ATGCAACTCA GTTCATGGAA TTTGAAAAAT TATCTCAGTT TGCAGCTGCG 
CTTGAACCGC CTCTCAATCT GCCACAACCA AACAAACTCC AGCTCATTGC CATGGATTTG 
CCCATGGTGA GTGGTGACCG GATCCACTGT CTTGATATCT TATTTGCTTT TACAAAGCGG 
GTTCTAGGAG AGAGTGGAGA GATGGATGCT CTACGAATAC AGATGGAAGA GCGATTCATG 
GCTTCCAATC CTTCCAAGGT CTCCTATCAG CCAATCACTA CTACTTTAAA ACGAAAACAA 
GAGGAAGTAT CTGCTGTCAT TATTCAGCGT GCTTACAGAC GCCACCTTTT AAAGCGAACT 
GTAAAACAAG CTTCCTTTAC GTACAATAAA AACAAAATCA AAGGTGGGGC TAATCTTCTT 
ATAAAAGAAG ACATGATAAT TGACAGAATA AATGAAAACT CTATTACAGA AAAAACTGAT 
CTGACCATGT CCACTGCAGC TTGTCCACCT TCCTATGACC GGGTGACAAA GCCAATTGTG 
GAAAAACATG AGCAAGAAGG CAAAGATGAA AAAGCCAAAG GGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001202435.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001189364.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 4, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	NM_001202435.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-06
Organism	Homo sapiens(Human)
Product	sodium channel protein type 1 subunit alpha isoform 1
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC107082.3 and AC010127.12. Transcript Variant: This variant (4) represents a transcript with 5' untranslated exons, compared to variant 1. The RefSeq Project created this record to better support clinical studies but the exact combination of exons and splice sites still needs to be experimentally confirmed. Variants 1 and 4 encode the same isoform. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1660803.90181.1, AB093548.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189364.1
CDS	228..6257
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001202435.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202435.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353948.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340877.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 5, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	NM_001353948.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. On Jun 2, 2019 this sequence version replaced NM_001353948.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.238456.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340877.1
CDS	300..6329
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353948.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353948.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353948.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340877.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 5, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	NM_001353948.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.238456.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340877.1
CDS	319..6348
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353948.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353948.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001165963.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159435.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 1, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	NM_001165963.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. On Jun 2, 2019 this sequence version replaced NM_001165963.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.90181.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159435.1
CDS	479..6508
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001165963.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165963.3

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001202435.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001189364.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 4, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	NM_001202435.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107082.3 and AC010127.12. On Jun 2, 2019 this sequence version replaced NM_001202435.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1803616.215809.1, AB093548.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189364.1
CDS	257..6286
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001202435.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202435.3

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001202435.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001189364.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 4, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	NM_001202435.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107082.3 and AC010127.12. On Aug 2, 2017 this sequence version replaced NM_001202435.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001189364.1
CDS	312..6341
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001202435.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001202435.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001165963.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159435.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 1, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	NM_001165963.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. On Aug 2, 2017 this sequence version replaced NM_001165963.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.90181.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159435.1
CDS	498..6527
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001165963.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165963.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001165963.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159435.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 1, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	NM_001165963.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-08
Organism	Homo sapiens(Human)
Product	sodium channel protein type 1 subunit alpha isoform 1
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12 and AB093548.1. Transcript Variant: This variant (1) encodes encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB093548.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159435.1
CDS	19..6048
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001165963.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165963.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_011511599.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509901.1 Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X2, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	XM_011511599.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	sodium channel protein type 1 subunit alpha isoform X3
Comment

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011509901.1

Translation

MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_011511599.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511599.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_017004644.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860133.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X1, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	XM_017004644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860133.1
CDS	474..6503
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017004644.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004644.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_011511602.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509904.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X2, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	XM_011511602.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511602.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509904.1
CDS	295..6324
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_011511602.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511602.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_011511598.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509900.1 Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X1, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	XM_011511598.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	sodium channel protein type 1 subunit alpha isoform X3
Comment

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011509900.1

Translation

MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_011511598.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511598.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_011511600.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509902.1 Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X3, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	XM_011511600.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	sodium channel protein type 1 subunit alpha isoform X3
Comment

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011509902.1

Translation

MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_011511600.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511600.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_011511601.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509903.1 Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X4, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	XM_011511601.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	sodium channel protein type 1 subunit alpha isoform X3
Comment

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011509903.1

Translation

MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_011511601.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511601.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_011511602.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509904.1 Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X5, mRNA.

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CloneID	OHu22576
Clone ID Related Accession (Same CDS sequence)	XM_011511599.1 , XM_017004644.1 , NM_001202435.1 , NM_001353948.2 , NM_001353948.1 , XM_011511602.2 , NM_001165963.3 , NM_001202435.3 , XM_011511598.1 , XM_011511600.1 , XM_011511601.1 , NM_001202435.2 , NM_001165963.2 , XM_011511602.1 , NM_001165963.1
Accession Version	XM_011511602.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	sodium channel protein type 1 subunit alpha isoform X3
Comment

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011509904.1

Translation

MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEVIIDKPATDDNGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_011511602.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens sodium channel, voltage gated, type I alpha subunit (SCN1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511602.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GTGATAATAG ATAAGCCAGC TACTGATGAC 
AATGGAACAA CCACTGAAAC TGAAATGAGA AAGAGAAGGT CAAGTTCTTT CCACGTTTCC 
ATGGACTTTC TAGAAGATCC TTCCCAAAGG CAACGAGCAA TGAGTATAGC CAGCATTCTA 
ACAAATACAG TAGAAGAACT TGAAGAATCC AGGCAGAAAT GCCCACCCTG TTGGTATAAA 
TTTTCCAACA TATTCTTAAT CTGGGACTGT TCTCCATATT GGTTAAAAGT GAAACATGTT 
GTCAACCTGG TTGTGATGGA CCCATTTGTT GACCTGGCCA TCACCATCTG TATTGTCTTA 
AATACTCTTT TCATGGCCAT GGAGCACTAT CCAATGACGG ACCATTTCAA TAATGTGCTT 
ACAGTAGGAA ACTTGGTTTT CACTGGGATC TTTACAGCAG AAATGTTTCT GAAAATTATT 
GCCATGGATC CTTACTATTA TTTCCAAGAA GGCTGGAATA TCTTTGACGG TTTTATTGTG 
ACGCTTAGCC TGGTAGAACT TGGACTCGCC AATGTGGAAG GATTATCTGT TCTCCGTTCA 
TTTCGATTGC TGCGAGTTTT CAAGTTGGCA AAATCTTGGC CAACGTTAAA TATGCTAATA 
AAGATCATCG GCAATTCCGT GGGGGCTCTG GGAAATTTAA CCCTCGTCTT GGCCATCATC 
GTCTTCATTT TTGCCGTGGT CGGCATGCAG CTCTTTGGTA AAAGCTACAA AGATTGTGTC 
TGCAAGATCG CCAGTGATTG TCAACTCCCA CGCTGGCACA TGAATGACTT CTTCCACTCC 
TTCCTGATTG TGTTCCGCGT GCTGTGTGGG GAGTGGATAG AGACCATGTG GGACTGTATG 
GAGGTTGCTG GTCAAGCCAT GTGCCTTACT GTCTTCATGA TGGTCATGGT GATTGGAAAC 
CTAGTGGTCC TGAATCTCTT TCTGGCCTTG CTTCTGAGCT CATTTAGTGC AGACAACCTT 
GCAGCCACTG ATGATGATAA TGAAATGAAT AATCTCCAAA TTGCTGTGGA TAGGATGCAC 
AAAGGAGTAG CTTATGTGAA AAGAAAAATA TATGAATTTA TTCAACAGTC CTTCATTAGG 
AAACAAAAGA TTTTAGATGA AATTAAACCA CTTGATGATC TAAACAACAA GAAAGACAGT 
TGTATGTCCA ATCATACAGC AGAAATTGGG AAAGATCTTG ACTATCTTAA AGATGTAAAT 
GGAACTACAA GTGGTATAGG AACTGGCAGC AGTGTTGAAA AATACATTAT TGATGAAAGT 
GATTACATGT CATTCATAAA CAACCCCAGT CTTACTGTGA CTGTACCAAT TGCTGTAGGA 
GAATCTGACT TTGAAAATTT AAACACGGAA GACTTTAGTA GTGAATCGGA TCTGGAAGAA 
AGCAAAGAGA AACTGAATGA AAGCAGTAGC TCATCAGAAG GTAGCACTGT GGACATCGGC 
GCACCTGTAG AAGAACAGCC CGTAGTGGAA CCTGAAGAAA CTCTTGAACC AGAAGCTTGT 
TTCACTGAAG GCTGTGTACA AAGATTCAAG TGTTGTCAAA TCAATGTGGA AGAAGGCAGA 
GGAAAACAAT GGTGGAACCT GAGAAGGACG TGTTTCCGAA TAGTTGAACA TAACTGGTTT 
GAGACCTTCA TTGTTTTCAT GATTCTCCTT AGTAGTGGTG CTCTGGCATT TGAAGATATA 
TATATTGATC AGCGAAAGAC GATTAAGACG ATGTTGGAAT ATGCTGACAA GGTTTTCACT 
TACATTTTCA TTCTGGAAAT GCTTCTAAAA TGGGTGGCAT ATGGCTATCA AACATATTTC 
ACCAATGCCT GGTGTTGGCT GGACTTCTTA ATTGTTGATG TTTCATTGGT CAGTTTAACA 
GCAAATGCCT TGGGTTACTC AGAACTTGGA GCCATCAAAT CTCTCAGGAC ACTAAGAGCT 
CTGAGACCTC TAAGAGCCTT ATCTCGATTT GAAGGGATGA GGGTGGTTGT GAATGCCCTT 
TTAGGAGCAA TTCCATCCAT CATGAATGTG CTTCTGGTTT GTCTTATATT CTGGCTAATT 
TTCAGCATCA TGGGCGTAAA TTTGTTTGCT GGCAAATTCT ACCACTGTAT TAACACCACA 
ACTGGTGACA GGTTTGACAT CGAAGACGTG AATAATCATA CTGATTGCCT AAAACTAATA 
GAAAGAAATG AGACTGCTCG ATGGAAAAAT GTGAAAGTAA ACTTTGATAA TGTAGGATTT 
GGGTATCTCT CTTTGCTTCA AGTTGCCACA TTCAAAGGAT GGATGGATAT AATGTATGCA 
GCAGTTGATT CCAGAAATGT GGAACTCCAG CCTAAGTATG AAGAAAGTCT GTACATGTAT 
CTTTACTTTG TTATTTTCAT CATCTTTGGG TCCTTCTTCA CCTTGAACCT GTTTATTGGT 
GTCATCATAG ATAATTTCAA CCAGCAGAAA AAGAAGTTTG GAGGTCAAGA CATCTTTATG 
ACAGAAGAAC AGAAGAAATA CTATAATGCA ATGAAAAAAT TAGGATCGAA AAAACCGCAA 
AAGCCTATAC CTCGACCAGG AAACAAATTT CAAGGAATGG TCTTTGACTT CGTAACCAGA 
CAAGTTTTTG ACATAAGCAT CATGATTCTC ATCTGTCTTA ACATGGTCAC AATGATGGTG 
GAAACAGATG ACCAGAGTGA ATATGTGACT ACCATTTTGT CACGCATCAA TCTGGTGTTC 
ATTGTGCTAT TTACTGGAGA GTGTGTACTG AAACTCATCT CTCTACGCCA TTATTATTTT 
ACCATTGGAT GGAATATTTT TGATTTTGTG GTTGTCATTC TCTCCATTGT AGGTATGTTT 
CTTGCCGAGC TGATAGAAAA GTATTTCGTG TCCCCTACCC TGTTCCGAGT GATCCGTCTT 
GCTAGGATTG GCCGAATCCT ACGTCTGATC AAAGGAGCAA AGGGGATCCG CACGCTGCTC 
TTTGCTTTGA TGATGTCCCT TCCTGCGTTG TTTAACATCG GCCTCCTACT CTTCCTAGTC 
ATGTTCATCT ACGCCATCTT TGGGATGTCC AACTTTGCCT ATGTTAAGAG GGAAGTTGGG 
ATCGATGACA TGTTCAACTT TGAGACCTTT GGCAACAGCA TGATCTGCCT ATTCCAAATT 
ACAACCTCTG CTGGCTGGGA TGGATTGCTA GCACCCATTC TCAACAGTAA GCCACCCGAC 
TGTGACCCTA ATAAAGTTAA CCCTGGAAGC TCAGTTAAGG GAGACTGTGG GAACCCATCT 
GTTGGAATTT TCTTTTTTGT CAGTTACATC ATCATATCCT TCCTGGTTGT GGTGAACATG 
TACATCGCGG TCATCCTGGA GAACTTCAGT GTTGCTACTG AAGAAAGTGC AGAGCCTCTG 
AGTGAGGATG ACTTTGAGAT GTTCTATGAG GTTTGGGAGA AGTTTGATCC CGATGCAACT 
CAGTTCATGG AATTTGAAAA ATTATCTCAG TTTGCAGCTG CGCTTGAACC GCCTCTCAAT 
CTGCCACAAC CAAACAAACT CCAGCTCATT GCCATGGATT TGCCCATGGT GAGTGGTGAC 
CGGATCCACT GTCTTGATAT CTTATTTGCT TTTACAAAGC GGGTTCTAGG AGAGAGTGGA 
GAGATGGATG CTCTACGAAT ACAGATGGAA GAGCGATTCA TGGCTTCCAA TCCTTCCAAG 
GTCTCCTATC AGCCAATCAC TACTACTTTA AAACGAAAAC AAGAGGAAGT ATCTGCTGTC 
ATTATTCAGC GTGCTTACAG ACGCCACCTT TTAAAGCGAA CTGTAAAACA AGCTTCCTTT 
ACGTACAATA AAAACAAAAT CAAAGGTGGG GCTAATCTTC TTATAAAAGA AGACATGATA 
ATTGACAGAA TAAATGAAAA CTCTATTACA GAAAAAACTG ATCTGACCAT GTCCACTGCA 
GCTTGTCCAC CTTCCTATGA CCGGGTGACA AAGCCAATTG TGGAAAAACA TGAGCAAGAA 
GGCAAAGATG AAAAAGCCAA AGGGAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353960.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340889.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 14, mRNA.

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CloneID	OHu89761
Clone ID Related Accession (Same CDS sequence)	NM_001353960.2 , XM_017004653.1 , NM_001353960.1
Accession Version	NM_001353960.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. On Jun 2, 2019 this sequence version replaced NM_001353960.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.88547.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGGAA CAACCACTGA AACTGAAATG 
AGAAAGAGAA GGTCAAGTTC TTTCCACGTT TCCATGGACT TTCTAGAAGA TCCTTCCCAA 
AGGCAACGAG CAATGAGTAT AGCCAGCATT CTAACAAATA CAGTAGAAGA ACTTGAAGAA 
TCCAGGCAGA AATGCCCACC CTGTTGGTAT AAATTTTCCA ACATATTCTT AATCTGGGAC 
TGTTCTCCAT ATTGGTTAAA AGTGAAACAT GTTGTCAACC TGGTTGTGAT GGACCCATTT 
GTTGACCTGG CCATCACCAT CTGTATTGTC TTAAATACTC TTTTCATGGC CATGGAGCAC 
TATCCAATGA CGGACCATTT CAATAATGTG CTTACAGTAG GAAACTTGGT TTTCACTGGG 
ATCTTTACAG CAGAAATGTT TCTGAAAATT ATTGCCATGG ATCCTTACTA TTATTTCCAA 
GAAGGCTGGA ATATCTTTGA CGGTTTTATT GTGACGCTTA GCCTGGTAGA ACTTGGACTC 
GCCAATGTGG AAGGATTATC TGTTCTCCGT TCATTTCGAT TGCTGCGAGT TTTCAAGTTG 
GCAAAATCTT GGCCAACGTT AAATATGCTA ATAAAGATCA TCGGCAATTC CGTGGGGGCT 
CTGGGAAATT TAACCCTCGT CTTGGCCATC ATCGTCTTCA TTTTTGCCGT GGTCGGCATG 
CAGCTCTTTG GTAAAAGCTA CAAAGATTGT GTCTGCAAGA TCGCCAGTGA TTGTCAACTC 
CCACGCTGGC ACATGAATGA CTTCTTCCAC TCCTTCCTGA TTGTGTTCCG CGTGCTGTGT 
GGGGAGTGGA TAGAGACCAT GTGGGACTGT ATGGAGGTTG CTGGTCAAGC CATGTGCCTT 
ACTGTCTTCA TGATGGTCAT GGTGATTGGA AACCTAGTGG TCCTGAATCT CTTTCTGGCC 
TTGCTTCTGA GCTCATTTAG TGCAGACAAC CTTGCAGCCA CTGATGATGA TAATGAAATG 
AATAATCTCC AAATTGCTGT GGATAGGATG CACAAAGGAG TAGCTTATGT GAAAAGAAAA 
ATATATGAAT TTATTCAACA GTCCTTCATT AGGAAACAAA AGATTTTAGA TGAAATTAAA 
CCACTTGATG ATCTAAACAA CAAGAAAGAC AGTTGTATGT CCAATCATAC AGCAGAAATT 
GGGAAAGATC TTGACTATCT TAAAGATGTA AATGGAACTA CAAGTGGTAT AGGAACTGGC 
AGCAGTGTTG AAAAATACAT TATTGATGAA AGTGATTACA TGTCATTCAT AAACAACCCC 
AGTCTTACTG TGACTGTACC AATTGCTGTA GGAGAATCTG ACTTTGAAAA TTTAAACACG 
GAAGACTTTA GTAGTGAATC GGATCTGGAA GAAAGCAAAG AGAAACTGAA TGAAAGCAGT 
AGCTCATCAG AAGGTAGCAC TGTGGACATC GGCGCACCTG TAGAAGAACA GCCCGTAGTG 
GAACCTGAAG AAACTCTTGA ACCAGAAGCT TGTTTCACTG AAGGCTGTGT ACAAAGATTC 
AAGTGTTGTC AAATCAATGT GGAAGAAGGC AGAGGAAAAC AATGGTGGAA CCTGAGAAGG 
ACGTGTTTCC GAATAGTTGA ACATAACTGG TTTGAGACCT TCATTGTTTT CATGATTCTC 
CTTAGTAGTG GTGCTCTGGC ATTTGAAGAT ATATATATTG ATCAGCGAAA GACGATTAAG 
ACGATGTTGG AATATGCTGA CAAGGTTTTC ACTTACATTT TCATTCTGGA AATGCTTCTA 
AAATGGGTGG CATATGGCTA TCAAACATAT TTCACCAATG CCTGGTGTTG GCTGGACTTC 
TTAATTGTTG ATGTTTCATT GGTCAGTTTA ACAGCAAATG CCTTGGGTTA CTCAGAACTT 
GGAGCCATCA AATCTCTCAG GACACTAAGA GCTCTGAGAC CTCTAAGAGC CTTATCTCGA 
TTTGAAGGGA TGAGGGTGGT TGTGAATGCC CTTTTAGGAG CAATTCCATC CATCATGAAT 
GTGCTTCTGG TTTGTCTTAT ATTCTGGCTA ATTTTCAGCA TCATGGGCGT AAATTTGTTT 
GCTGGCAAAT TCTACCACTG TATTAACACC ACAACTGGTG ACAGGTTTGA CATCGAAGAC 
GTGAATAATC ATACTGATTG CCTAAAACTA ATAGAAAGAA ATGAGACTGC TCGATGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA TTTGGGTATC TCTCTTTGCT TCAAGTTGCC 
ACATTCAAAG GATGGATGGA TATAATGTAT GCAGCAGTTG ATTCCAGAAA TGTGGAACTC 
CAGCCTAAGT ATGAAGAAAG TCTGTACATG TATCTTTACT TTGTTATTTT CATCATCTTT 
GGGTCCTTCT TCACCTTGAA CCTGTTTATT GGTGTCATCA TAGATAATTT CAACCAGCAG 
AAAAAGAAGT TTGGAGGTCA AGACATCTTT ATGACAGAAG AACAGAAGAA ATACTATAAT 
GCAATGAAAA AATTAGGATC GAAAAAACCG CAAAAGCCTA TACCTCGACC AGGAAACAAA 
TTTCAAGGAA TGGTCTTTGA CTTCGTAACC AGACAAGTTT TTGACATAAG CATCATGATT 
CTCATCTGTC TTAACATGGT CACAATGATG GTGGAAACAG ATGACCAGAG TGAATATGTG 
ACTACCATTT TGTCACGCAT CAATCTGGTG TTCATTGTGC TATTTACTGG AGAGTGTGTA 
CTGAAACTCA TCTCTCTACG CCATTATTAT TTTACCATTG GATGGAATAT TTTTGATTTT 
GTGGTTGTCA TTCTCTCCAT TGTAGGTATG TTTCTTGCCG AGCTGATAGA AAAGTATTTC 
GTGTCCCCTA CCCTGTTCCG AGTGATCCGT CTTGCTAGGA TTGGCCGAAT CCTACGTCTG 
ATCAAAGGAG CAAAGGGGAT CCGCACGCTG CTCTTTGCTT TGATGATGTC CCTTCCTGCG 
TTGTTTAACA TCGGCCTCCT ACTCTTCCTA GTCATGTTCA TCTACGCCAT CTTTGGGATG 
TCCAACTTTG CCTATGTTAA GAGGGAAGTT GGGATCGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CCTATTCCAA ATTACAACCT CTGCTGGCTG GGATGGATTG 
CTAGCACCCA TTCTCAACAG TAAGCCACCC GACTGTGACC CTAATAAAGT TAACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTTGGAA TTTTCTTTTT TGTCAGTTAC 
ATCATCATAT CCTTCCTGGT TGTGGTGAAC ATGTACATCG CGGTCATCCT GGAGAACTTC 
AGTGTTGCTA CTGAAGAAAG TGCAGAGCCT CTGAGTGAGG ATGACTTTGA GATGTTCTAT 
GAGGTTTGGG AGAAGTTTGA TCCCGATGCA ACTCAGTTCA TGGAATTTGA AAAATTATCT 
CAGTTTGCAG CTGCGCTTGA ACCGCCTCTC AATCTGCCAC AACCAAACAA ACTCCAGCTC 
ATTGCCATGG ATTTGCCCAT GGTGAGTGGT GACCGGATCC ACTGTCTTGA TATCTTATTT 
GCTTTTACAA AGCGGGTTCT AGGAGAGAGT GGAGAGATGG ATGCTCTACG AATACAGATG 
GAAGAGCGAT TCATGGCTTC CAATCCTTCC AAGGTCTCCT ATCAGCCAAT CACTACTACT 
TTAAAACGAA AACAAGAGGA AGTATCTGCT GTCATTATTC AGCGTGCTTA CAGACGCCAC 
CTTTTAAAGC GAACTGTAAA ACAAGCTTCC TTTACGTACA ATAAAAACAA AATCAAAGGT 
GGGGCTAATC TTCTTATAAA AGAAGACATG ATAATTGACA GAATAAATGA AAACTCTATT 
ACAGAAAAAA CTGATCTGAC CATGTCCACT GCAGCTTGTC CACCTTCCTA TGACCGGGTG 
ACAAAGCCAA TTGTGGAAAA ACATGAGCAA GAAGGCAAAG ATGAAAAAGC CAAAGGGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340889.1
CDS	392..6334
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353960.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353960.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGGAA CAACCACTGA AACTGAAATG 
AGAAAGAGAA GGTCAAGTTC TTTCCACGTT TCCATGGACT TTCTAGAAGA TCCTTCCCAA 
AGGCAACGAG CAATGAGTAT AGCCAGCATT CTAACAAATA CAGTAGAAGA ACTTGAAGAA 
TCCAGGCAGA AATGCCCACC CTGTTGGTAT AAATTTTCCA ACATATTCTT AATCTGGGAC 
TGTTCTCCAT ATTGGTTAAA AGTGAAACAT GTTGTCAACC TGGTTGTGAT GGACCCATTT 
GTTGACCTGG CCATCACCAT CTGTATTGTC TTAAATACTC TTTTCATGGC CATGGAGCAC 
TATCCAATGA CGGACCATTT CAATAATGTG CTTACAGTAG GAAACTTGGT TTTCACTGGG 
ATCTTTACAG CAGAAATGTT TCTGAAAATT ATTGCCATGG ATCCTTACTA TTATTTCCAA 
GAAGGCTGGA ATATCTTTGA CGGTTTTATT GTGACGCTTA GCCTGGTAGA ACTTGGACTC 
GCCAATGTGG AAGGATTATC TGTTCTCCGT TCATTTCGAT TGCTGCGAGT TTTCAAGTTG 
GCAAAATCTT GGCCAACGTT AAATATGCTA ATAAAGATCA TCGGCAATTC CGTGGGGGCT 
CTGGGAAATT TAACCCTCGT CTTGGCCATC ATCGTCTTCA TTTTTGCCGT GGTCGGCATG 
CAGCTCTTTG GTAAAAGCTA CAAAGATTGT GTCTGCAAGA TCGCCAGTGA TTGTCAACTC 
CCACGCTGGC ACATGAATGA CTTCTTCCAC TCCTTCCTGA TTGTGTTCCG CGTGCTGTGT 
GGGGAGTGGA TAGAGACCAT GTGGGACTGT ATGGAGGTTG CTGGTCAAGC CATGTGCCTT 
ACTGTCTTCA TGATGGTCAT GGTGATTGGA AACCTAGTGG TCCTGAATCT CTTTCTGGCC 
TTGCTTCTGA GCTCATTTAG TGCAGACAAC CTTGCAGCCA CTGATGATGA TAATGAAATG 
AATAATCTCC AAATTGCTGT GGATAGGATG CACAAAGGAG TAGCTTATGT GAAAAGAAAA 
ATATATGAAT TTATTCAACA GTCCTTCATT AGGAAACAAA AGATTTTAGA TGAAATTAAA 
CCACTTGATG ATCTAAACAA CAAGAAAGAC AGTTGTATGT CCAATCATAC AGCAGAAATT 
GGGAAAGATC TTGACTATCT TAAAGATGTA AATGGAACTA CAAGTGGTAT AGGAACTGGC 
AGCAGTGTTG AAAAATACAT TATTGATGAA AGTGATTACA TGTCATTCAT AAACAACCCC 
AGTCTTACTG TGACTGTACC AATTGCTGTA GGAGAATCTG ACTTTGAAAA TTTAAACACG 
GAAGACTTTA GTAGTGAATC GGATCTGGAA GAAAGCAAAG AGAAACTGAA TGAAAGCAGT 
AGCTCATCAG AAGGTAGCAC TGTGGACATC GGCGCACCTG TAGAAGAACA GCCCGTAGTG 
GAACCTGAAG AAACTCTTGA ACCAGAAGCT TGTTTCACTG AAGGCTGTGT ACAAAGATTC 
AAGTGTTGTC AAATCAATGT GGAAGAAGGC AGAGGAAAAC AATGGTGGAA CCTGAGAAGG 
ACGTGTTTCC GAATAGTTGA ACATAACTGG TTTGAGACCT TCATTGTTTT CATGATTCTC 
CTTAGTAGTG GTGCTCTGGC ATTTGAAGAT ATATATATTG ATCAGCGAAA GACGATTAAG 
ACGATGTTGG AATATGCTGA CAAGGTTTTC ACTTACATTT TCATTCTGGA AATGCTTCTA 
AAATGGGTGG CATATGGCTA TCAAACATAT TTCACCAATG CCTGGTGTTG GCTGGACTTC 
TTAATTGTTG ATGTTTCATT GGTCAGTTTA ACAGCAAATG CCTTGGGTTA CTCAGAACTT 
GGAGCCATCA AATCTCTCAG GACACTAAGA GCTCTGAGAC CTCTAAGAGC CTTATCTCGA 
TTTGAAGGGA TGAGGGTGGT TGTGAATGCC CTTTTAGGAG CAATTCCATC CATCATGAAT 
GTGCTTCTGG TTTGTCTTAT ATTCTGGCTA ATTTTCAGCA TCATGGGCGT AAATTTGTTT 
GCTGGCAAAT TCTACCACTG TATTAACACC ACAACTGGTG ACAGGTTTGA CATCGAAGAC 
GTGAATAATC ATACTGATTG CCTAAAACTA ATAGAAAGAA ATGAGACTGC TCGATGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA TTTGGGTATC TCTCTTTGCT TCAAGTTGCC 
ACATTCAAAG GATGGATGGA TATAATGTAT GCAGCAGTTG ATTCCAGAAA TGTGGAACTC 
CAGCCTAAGT ATGAAGAAAG TCTGTACATG TATCTTTACT TTGTTATTTT CATCATCTTT 
GGGTCCTTCT TCACCTTGAA CCTGTTTATT GGTGTCATCA TAGATAATTT CAACCAGCAG 
AAAAAGAAGT TTGGAGGTCA AGACATCTTT ATGACAGAAG AACAGAAGAA ATACTATAAT 
GCAATGAAAA AATTAGGATC GAAAAAACCG CAAAAGCCTA TACCTCGACC AGGAAACAAA 
TTTCAAGGAA TGGTCTTTGA CTTCGTAACC AGACAAGTTT TTGACATAAG CATCATGATT 
CTCATCTGTC TTAACATGGT CACAATGATG GTGGAAACAG ATGACCAGAG TGAATATGTG 
ACTACCATTT TGTCACGCAT CAATCTGGTG TTCATTGTGC TATTTACTGG AGAGTGTGTA 
CTGAAACTCA TCTCTCTACG CCATTATTAT TTTACCATTG GATGGAATAT TTTTGATTTT 
GTGGTTGTCA TTCTCTCCAT TGTAGGTATG TTTCTTGCCG AGCTGATAGA AAAGTATTTC 
GTGTCCCCTA CCCTGTTCCG AGTGATCCGT CTTGCTAGGA TTGGCCGAAT CCTACGTCTG 
ATCAAAGGAG CAAAGGGGAT CCGCACGCTG CTCTTTGCTT TGATGATGTC CCTTCCTGCG 
TTGTTTAACA TCGGCCTCCT ACTCTTCCTA GTCATGTTCA TCTACGCCAT CTTTGGGATG 
TCCAACTTTG CCTATGTTAA GAGGGAAGTT GGGATCGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CCTATTCCAA ATTACAACCT CTGCTGGCTG GGATGGATTG 
CTAGCACCCA TTCTCAACAG TAAGCCACCC GACTGTGACC CTAATAAAGT TAACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTTGGAA TTTTCTTTTT TGTCAGTTAC 
ATCATCATAT CCTTCCTGGT TGTGGTGAAC ATGTACATCG CGGTCATCCT GGAGAACTTC 
AGTGTTGCTA CTGAAGAAAG TGCAGAGCCT CTGAGTGAGG ATGACTTTGA GATGTTCTAT 
GAGGTTTGGG AGAAGTTTGA TCCCGATGCA ACTCAGTTCA TGGAATTTGA AAAATTATCT 
CAGTTTGCAG CTGCGCTTGA ACCGCCTCTC AATCTGCCAC AACCAAACAA ACTCCAGCTC 
ATTGCCATGG ATTTGCCCAT GGTGAGTGGT GACCGGATCC ACTGTCTTGA TATCTTATTT 
GCTTTTACAA AGCGGGTTCT AGGAGAGAGT GGAGAGATGG ATGCTCTACG AATACAGATG 
GAAGAGCGAT TCATGGCTTC CAATCCTTCC AAGGTCTCCT ATCAGCCAAT CACTACTACT 
TTAAAACGAA AACAAGAGGA AGTATCTGCT GTCATTATTC AGCGTGCTTA CAGACGCCAC 
CTTTTAAAGC GAACTGTAAA ACAAGCTTCC TTTACGTACA ATAAAAACAA AATCAAAGGT 
GGGGCTAATC TTCTTATAAA AGAAGACATG ATAATTGACA GAATAAATGA AAACTCTATT 
ACAGAAAAAA CTGATCTGAC CATGTCCACT GCAGCTTGTC CACCTTCCTA TGACCGGGTG 
ACAAAGCCAA TTGTGGAAAA ACATGAGCAA GAAGGCAAAG ATGAAAAAGC CAAAGGGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353960.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340889.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 14, mRNA.

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CloneID	OHu89761
Clone ID Related Accession (Same CDS sequence)	NM_001353960.2 , XM_017004653.1 , NM_001353960.1
Accession Version	NM_001353960.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.88547.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGGAA CAACCACTGA AACTGAAATG 
AGAAAGAGAA GGTCAAGTTC TTTCCACGTT TCCATGGACT TTCTAGAAGA TCCTTCCCAA 
AGGCAACGAG CAATGAGTAT AGCCAGCATT CTAACAAATA CAGTAGAAGA ACTTGAAGAA 
TCCAGGCAGA AATGCCCACC CTGTTGGTAT AAATTTTCCA ACATATTCTT AATCTGGGAC 
TGTTCTCCAT ATTGGTTAAA AGTGAAACAT GTTGTCAACC TGGTTGTGAT GGACCCATTT 
GTTGACCTGG CCATCACCAT CTGTATTGTC TTAAATACTC TTTTCATGGC CATGGAGCAC 
TATCCAATGA CGGACCATTT CAATAATGTG CTTACAGTAG GAAACTTGGT TTTCACTGGG 
ATCTTTACAG CAGAAATGTT TCTGAAAATT ATTGCCATGG ATCCTTACTA TTATTTCCAA 
GAAGGCTGGA ATATCTTTGA CGGTTTTATT GTGACGCTTA GCCTGGTAGA ACTTGGACTC 
GCCAATGTGG AAGGATTATC TGTTCTCCGT TCATTTCGAT TGCTGCGAGT TTTCAAGTTG 
GCAAAATCTT GGCCAACGTT AAATATGCTA ATAAAGATCA TCGGCAATTC CGTGGGGGCT 
CTGGGAAATT TAACCCTCGT CTTGGCCATC ATCGTCTTCA TTTTTGCCGT GGTCGGCATG 
CAGCTCTTTG GTAAAAGCTA CAAAGATTGT GTCTGCAAGA TCGCCAGTGA TTGTCAACTC 
CCACGCTGGC ACATGAATGA CTTCTTCCAC TCCTTCCTGA TTGTGTTCCG CGTGCTGTGT 
GGGGAGTGGA TAGAGACCAT GTGGGACTGT ATGGAGGTTG CTGGTCAAGC CATGTGCCTT 
ACTGTCTTCA TGATGGTCAT GGTGATTGGA AACCTAGTGG TCCTGAATCT CTTTCTGGCC 
TTGCTTCTGA GCTCATTTAG TGCAGACAAC CTTGCAGCCA CTGATGATGA TAATGAAATG 
AATAATCTCC AAATTGCTGT GGATAGGATG CACAAAGGAG TAGCTTATGT GAAAAGAAAA 
ATATATGAAT TTATTCAACA GTCCTTCATT AGGAAACAAA AGATTTTAGA TGAAATTAAA 
CCACTTGATG ATCTAAACAA CAAGAAAGAC AGTTGTATGT CCAATCATAC AGCAGAAATT 
GGGAAAGATC TTGACTATCT TAAAGATGTA AATGGAACTA CAAGTGGTAT AGGAACTGGC 
AGCAGTGTTG AAAAATACAT TATTGATGAA AGTGATTACA TGTCATTCAT AAACAACCCC 
AGTCTTACTG TGACTGTACC AATTGCTGTA GGAGAATCTG ACTTTGAAAA TTTAAACACG 
GAAGACTTTA GTAGTGAATC GGATCTGGAA GAAAGCAAAG AGAAACTGAA TGAAAGCAGT 
AGCTCATCAG AAGGTAGCAC TGTGGACATC GGCGCACCTG TAGAAGAACA GCCCGTAGTG 
GAACCTGAAG AAACTCTTGA ACCAGAAGCT TGTTTCACTG AAGGCTGTGT ACAAAGATTC 
AAGTGTTGTC AAATCAATGT GGAAGAAGGC AGAGGAAAAC AATGGTGGAA CCTGAGAAGG 
ACGTGTTTCC GAATAGTTGA ACATAACTGG TTTGAGACCT TCATTGTTTT CATGATTCTC 
CTTAGTAGTG GTGCTCTGGC ATTTGAAGAT ATATATATTG ATCAGCGAAA GACGATTAAG 
ACGATGTTGG AATATGCTGA CAAGGTTTTC ACTTACATTT TCATTCTGGA AATGCTTCTA 
AAATGGGTGG CATATGGCTA TCAAACATAT TTCACCAATG CCTGGTGTTG GCTGGACTTC 
TTAATTGTTG ATGTTTCATT GGTCAGTTTA ACAGCAAATG CCTTGGGTTA CTCAGAACTT 
GGAGCCATCA AATCTCTCAG GACACTAAGA GCTCTGAGAC CTCTAAGAGC CTTATCTCGA 
TTTGAAGGGA TGAGGGTGGT TGTGAATGCC CTTTTAGGAG CAATTCCATC CATCATGAAT 
GTGCTTCTGG TTTGTCTTAT ATTCTGGCTA ATTTTCAGCA TCATGGGCGT AAATTTGTTT 
GCTGGCAAAT TCTACCACTG TATTAACACC ACAACTGGTG ACAGGTTTGA CATCGAAGAC 
GTGAATAATC ATACTGATTG CCTAAAACTA ATAGAAAGAA ATGAGACTGC TCGATGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA TTTGGGTATC TCTCTTTGCT TCAAGTTGCC 
ACATTCAAAG GATGGATGGA TATAATGTAT GCAGCAGTTG ATTCCAGAAA TGTGGAACTC 
CAGCCTAAGT ATGAAGAAAG TCTGTACATG TATCTTTACT TTGTTATTTT CATCATCTTT 
GGGTCCTTCT TCACCTTGAA CCTGTTTATT GGTGTCATCA TAGATAATTT CAACCAGCAG 
AAAAAGAAGT TTGGAGGTCA AGACATCTTT ATGACAGAAG AACAGAAGAA ATACTATAAT 
GCAATGAAAA AATTAGGATC GAAAAAACCG CAAAAGCCTA TACCTCGACC AGGAAACAAA 
TTTCAAGGAA TGGTCTTTGA CTTCGTAACC AGACAAGTTT TTGACATAAG CATCATGATT 
CTCATCTGTC TTAACATGGT CACAATGATG GTGGAAACAG ATGACCAGAG TGAATATGTG 
ACTACCATTT TGTCACGCAT CAATCTGGTG TTCATTGTGC TATTTACTGG AGAGTGTGTA 
CTGAAACTCA TCTCTCTACG CCATTATTAT TTTACCATTG GATGGAATAT TTTTGATTTT 
GTGGTTGTCA TTCTCTCCAT TGTAGGTATG TTTCTTGCCG AGCTGATAGA AAAGTATTTC 
GTGTCCCCTA CCCTGTTCCG AGTGATCCGT CTTGCTAGGA TTGGCCGAAT CCTACGTCTG 
ATCAAAGGAG CAAAGGGGAT CCGCACGCTG CTCTTTGCTT TGATGATGTC CCTTCCTGCG 
TTGTTTAACA TCGGCCTCCT ACTCTTCCTA GTCATGTTCA TCTACGCCAT CTTTGGGATG 
TCCAACTTTG CCTATGTTAA GAGGGAAGTT GGGATCGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CCTATTCCAA ATTACAACCT CTGCTGGCTG GGATGGATTG 
CTAGCACCCA TTCTCAACAG TAAGCCACCC GACTGTGACC CTAATAAAGT TAACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTTGGAA TTTTCTTTTT TGTCAGTTAC 
ATCATCATAT CCTTCCTGGT TGTGGTGAAC ATGTACATCG CGGTCATCCT GGAGAACTTC 
AGTGTTGCTA CTGAAGAAAG TGCAGAGCCT CTGAGTGAGG ATGACTTTGA GATGTTCTAT 
GAGGTTTGGG AGAAGTTTGA TCCCGATGCA ACTCAGTTCA TGGAATTTGA AAAATTATCT 
CAGTTTGCAG CTGCGCTTGA ACCGCCTCTC AATCTGCCAC AACCAAACAA ACTCCAGCTC 
ATTGCCATGG ATTTGCCCAT GGTGAGTGGT GACCGGATCC ACTGTCTTGA TATCTTATTT 
GCTTTTACAA AGCGGGTTCT AGGAGAGAGT GGAGAGATGG ATGCTCTACG AATACAGATG 
GAAGAGCGAT TCATGGCTTC CAATCCTTCC AAGGTCTCCT ATCAGCCAAT CACTACTACT 
TTAAAACGAA AACAAGAGGA AGTATCTGCT GTCATTATTC AGCGTGCTTA CAGACGCCAC 
CTTTTAAAGC GAACTGTAAA ACAAGCTTCC TTTACGTACA ATAAAAACAA AATCAAAGGT 
GGGGCTAATC TTCTTATAAA AGAAGACATG ATAATTGACA GAATAAATGA AAACTCTATT 
ACAGAAAAAA CTGATCTGAC CATGTCCACT GCAGCTTGTC CACCTTCCTA TGACCGGGTG 
ACAAAGCCAA TTGTGGAAAA ACATGAGCAA GAAGGCAAAG ATGAAAAAGC CAAAGGGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340889.1
CDS	411..6353
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353960.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353960.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGGAA CAACCACTGA AACTGAAATG 
AGAAAGAGAA GGTCAAGTTC TTTCCACGTT TCCATGGACT TTCTAGAAGA TCCTTCCCAA 
AGGCAACGAG CAATGAGTAT AGCCAGCATT CTAACAAATA CAGTAGAAGA ACTTGAAGAA 
TCCAGGCAGA AATGCCCACC CTGTTGGTAT AAATTTTCCA ACATATTCTT AATCTGGGAC 
TGTTCTCCAT ATTGGTTAAA AGTGAAACAT GTTGTCAACC TGGTTGTGAT GGACCCATTT 
GTTGACCTGG CCATCACCAT CTGTATTGTC TTAAATACTC TTTTCATGGC CATGGAGCAC 
TATCCAATGA CGGACCATTT CAATAATGTG CTTACAGTAG GAAACTTGGT TTTCACTGGG 
ATCTTTACAG CAGAAATGTT TCTGAAAATT ATTGCCATGG ATCCTTACTA TTATTTCCAA 
GAAGGCTGGA ATATCTTTGA CGGTTTTATT GTGACGCTTA GCCTGGTAGA ACTTGGACTC 
GCCAATGTGG AAGGATTATC TGTTCTCCGT TCATTTCGAT TGCTGCGAGT TTTCAAGTTG 
GCAAAATCTT GGCCAACGTT AAATATGCTA ATAAAGATCA TCGGCAATTC CGTGGGGGCT 
CTGGGAAATT TAACCCTCGT CTTGGCCATC ATCGTCTTCA TTTTTGCCGT GGTCGGCATG 
CAGCTCTTTG GTAAAAGCTA CAAAGATTGT GTCTGCAAGA TCGCCAGTGA TTGTCAACTC 
CCACGCTGGC ACATGAATGA CTTCTTCCAC TCCTTCCTGA TTGTGTTCCG CGTGCTGTGT 
GGGGAGTGGA TAGAGACCAT GTGGGACTGT ATGGAGGTTG CTGGTCAAGC CATGTGCCTT 
ACTGTCTTCA TGATGGTCAT GGTGATTGGA AACCTAGTGG TCCTGAATCT CTTTCTGGCC 
TTGCTTCTGA GCTCATTTAG TGCAGACAAC CTTGCAGCCA CTGATGATGA TAATGAAATG 
AATAATCTCC AAATTGCTGT GGATAGGATG CACAAAGGAG TAGCTTATGT GAAAAGAAAA 
ATATATGAAT TTATTCAACA GTCCTTCATT AGGAAACAAA AGATTTTAGA TGAAATTAAA 
CCACTTGATG ATCTAAACAA CAAGAAAGAC AGTTGTATGT CCAATCATAC AGCAGAAATT 
GGGAAAGATC TTGACTATCT TAAAGATGTA AATGGAACTA CAAGTGGTAT AGGAACTGGC 
AGCAGTGTTG AAAAATACAT TATTGATGAA AGTGATTACA TGTCATTCAT AAACAACCCC 
AGTCTTACTG TGACTGTACC AATTGCTGTA GGAGAATCTG ACTTTGAAAA TTTAAACACG 
GAAGACTTTA GTAGTGAATC GGATCTGGAA GAAAGCAAAG AGAAACTGAA TGAAAGCAGT 
AGCTCATCAG AAGGTAGCAC TGTGGACATC GGCGCACCTG TAGAAGAACA GCCCGTAGTG 
GAACCTGAAG AAACTCTTGA ACCAGAAGCT TGTTTCACTG AAGGCTGTGT ACAAAGATTC 
AAGTGTTGTC AAATCAATGT GGAAGAAGGC AGAGGAAAAC AATGGTGGAA CCTGAGAAGG 
ACGTGTTTCC GAATAGTTGA ACATAACTGG TTTGAGACCT TCATTGTTTT CATGATTCTC 
CTTAGTAGTG GTGCTCTGGC ATTTGAAGAT ATATATATTG ATCAGCGAAA GACGATTAAG 
ACGATGTTGG AATATGCTGA CAAGGTTTTC ACTTACATTT TCATTCTGGA AATGCTTCTA 
AAATGGGTGG CATATGGCTA TCAAACATAT TTCACCAATG CCTGGTGTTG GCTGGACTTC 
TTAATTGTTG ATGTTTCATT GGTCAGTTTA ACAGCAAATG CCTTGGGTTA CTCAGAACTT 
GGAGCCATCA AATCTCTCAG GACACTAAGA GCTCTGAGAC CTCTAAGAGC CTTATCTCGA 
TTTGAAGGGA TGAGGGTGGT TGTGAATGCC CTTTTAGGAG CAATTCCATC CATCATGAAT 
GTGCTTCTGG TTTGTCTTAT ATTCTGGCTA ATTTTCAGCA TCATGGGCGT AAATTTGTTT 
GCTGGCAAAT TCTACCACTG TATTAACACC ACAACTGGTG ACAGGTTTGA CATCGAAGAC 
GTGAATAATC ATACTGATTG CCTAAAACTA ATAGAAAGAA ATGAGACTGC TCGATGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA TTTGGGTATC TCTCTTTGCT TCAAGTTGCC 
ACATTCAAAG GATGGATGGA TATAATGTAT GCAGCAGTTG ATTCCAGAAA TGTGGAACTC 
CAGCCTAAGT ATGAAGAAAG TCTGTACATG TATCTTTACT TTGTTATTTT CATCATCTTT 
GGGTCCTTCT TCACCTTGAA CCTGTTTATT GGTGTCATCA TAGATAATTT CAACCAGCAG 
AAAAAGAAGT TTGGAGGTCA AGACATCTTT ATGACAGAAG AACAGAAGAA ATACTATAAT 
GCAATGAAAA AATTAGGATC GAAAAAACCG CAAAAGCCTA TACCTCGACC AGGAAACAAA 
TTTCAAGGAA TGGTCTTTGA CTTCGTAACC AGACAAGTTT TTGACATAAG CATCATGATT 
CTCATCTGTC TTAACATGGT CACAATGATG GTGGAAACAG ATGACCAGAG TGAATATGTG 
ACTACCATTT TGTCACGCAT CAATCTGGTG TTCATTGTGC TATTTACTGG AGAGTGTGTA 
CTGAAACTCA TCTCTCTACG CCATTATTAT TTTACCATTG GATGGAATAT TTTTGATTTT 
GTGGTTGTCA TTCTCTCCAT TGTAGGTATG TTTCTTGCCG AGCTGATAGA AAAGTATTTC 
GTGTCCCCTA CCCTGTTCCG AGTGATCCGT CTTGCTAGGA TTGGCCGAAT CCTACGTCTG 
ATCAAAGGAG CAAAGGGGAT CCGCACGCTG CTCTTTGCTT TGATGATGTC CCTTCCTGCG 
TTGTTTAACA TCGGCCTCCT ACTCTTCCTA GTCATGTTCA TCTACGCCAT CTTTGGGATG 
TCCAACTTTG CCTATGTTAA GAGGGAAGTT GGGATCGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CCTATTCCAA ATTACAACCT CTGCTGGCTG GGATGGATTG 
CTAGCACCCA TTCTCAACAG TAAGCCACCC GACTGTGACC CTAATAAAGT TAACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTTGGAA TTTTCTTTTT TGTCAGTTAC 
ATCATCATAT CCTTCCTGGT TGTGGTGAAC ATGTACATCG CGGTCATCCT GGAGAACTTC 
AGTGTTGCTA CTGAAGAAAG TGCAGAGCCT CTGAGTGAGG ATGACTTTGA GATGTTCTAT 
GAGGTTTGGG AGAAGTTTGA TCCCGATGCA ACTCAGTTCA TGGAATTTGA AAAATTATCT 
CAGTTTGCAG CTGCGCTTGA ACCGCCTCTC AATCTGCCAC AACCAAACAA ACTCCAGCTC 
ATTGCCATGG ATTTGCCCAT GGTGAGTGGT GACCGGATCC ACTGTCTTGA TATCTTATTT 
GCTTTTACAA AGCGGGTTCT AGGAGAGAGT GGAGAGATGG ATGCTCTACG AATACAGATG 
GAAGAGCGAT TCATGGCTTC CAATCCTTCC AAGGTCTCCT ATCAGCCAAT CACTACTACT 
TTAAAACGAA AACAAGAGGA AGTATCTGCT GTCATTATTC AGCGTGCTTA CAGACGCCAC 
CTTTTAAAGC GAACTGTAAA ACAAGCTTCC TTTACGTACA ATAAAAACAA AATCAAAGGT 
GGGGCTAATC TTCTTATAAA AGAAGACATG ATAATTGACA GAATAAATGA AAACTCTATT 
ACAGAAAAAA CTGATCTGAC CATGTCCACT GCAGCTTGTC CACCTTCCTA TGACCGGGTG 
ACAAAGCCAA TTGTGGAAAA ACATGAGCAA GAAGGCAAAG ATGAAAAAGC CAAAGGGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_017004653.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860142.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X14, mRNA.

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CloneID	OHu89761
Clone ID Related Accession (Same CDS sequence)	NM_001353960.2 , XM_017004653.1 , NM_001353960.1
Accession Version	XM_017004653.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5943bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGGAA CAACCACTGA AACTGAAATG 
AGAAAGAGAA GGTCAAGTTC TTTCCACGTT TCCATGGACT TTCTAGAAGA TCCTTCCCAA 
AGGCAACGAG CAATGAGTAT AGCCAGCATT CTAACAAATA CAGTAGAAGA ACTTGAAGAA 
TCCAGGCAGA AATGCCCACC CTGTTGGTAT AAATTTTCCA ACATATTCTT AATCTGGGAC 
TGTTCTCCAT ATTGGTTAAA AGTGAAACAT GTTGTCAACC TGGTTGTGAT GGACCCATTT 
GTTGACCTGG CCATCACCAT CTGTATTGTC TTAAATACTC TTTTCATGGC CATGGAGCAC 
TATCCAATGA CGGACCATTT CAATAATGTG CTTACAGTAG GAAACTTGGT TTTCACTGGG 
ATCTTTACAG CAGAAATGTT TCTGAAAATT ATTGCCATGG ATCCTTACTA TTATTTCCAA 
GAAGGCTGGA ATATCTTTGA CGGTTTTATT GTGACGCTTA GCCTGGTAGA ACTTGGACTC 
GCCAATGTGG AAGGATTATC TGTTCTCCGT TCATTTCGAT TGCTGCGAGT TTTCAAGTTG 
GCAAAATCTT GGCCAACGTT AAATATGCTA ATAAAGATCA TCGGCAATTC CGTGGGGGCT 
CTGGGAAATT TAACCCTCGT CTTGGCCATC ATCGTCTTCA TTTTTGCCGT GGTCGGCATG 
CAGCTCTTTG GTAAAAGCTA CAAAGATTGT GTCTGCAAGA TCGCCAGTGA TTGTCAACTC 
CCACGCTGGC ACATGAATGA CTTCTTCCAC TCCTTCCTGA TTGTGTTCCG CGTGCTGTGT 
GGGGAGTGGA TAGAGACCAT GTGGGACTGT ATGGAGGTTG CTGGTCAAGC CATGTGCCTT 
ACTGTCTTCA TGATGGTCAT GGTGATTGGA AACCTAGTGG TCCTGAATCT CTTTCTGGCC 
TTGCTTCTGA GCTCATTTAG TGCAGACAAC CTTGCAGCCA CTGATGATGA TAATGAAATG 
AATAATCTCC AAATTGCTGT GGATAGGATG CACAAAGGAG TAGCTTATGT GAAAAGAAAA 
ATATATGAAT TTATTCAACA GTCCTTCATT AGGAAACAAA AGATTTTAGA TGAAATTAAA 
CCACTTGATG ATCTAAACAA CAAGAAAGAC AGTTGTATGT CCAATCATAC AGCAGAAATT 
GGGAAAGATC TTGACTATCT TAAAGATGTA AATGGAACTA CAAGTGGTAT AGGAACTGGC 
AGCAGTGTTG AAAAATACAT TATTGATGAA AGTGATTACA TGTCATTCAT AAACAACCCC 
AGTCTTACTG TGACTGTACC AATTGCTGTA GGAGAATCTG ACTTTGAAAA TTTAAACACG 
GAAGACTTTA GTAGTGAATC GGATCTGGAA GAAAGCAAAG AGAAACTGAA TGAAAGCAGT 
AGCTCATCAG AAGGTAGCAC TGTGGACATC GGCGCACCTG TAGAAGAACA GCCCGTAGTG 
GAACCTGAAG AAACTCTTGA ACCAGAAGCT TGTTTCACTG AAGGCTGTGT ACAAAGATTC 
AAGTGTTGTC AAATCAATGT GGAAGAAGGC AGAGGAAAAC AATGGTGGAA CCTGAGAAGG 
ACGTGTTTCC GAATAGTTGA ACATAACTGG TTTGAGACCT TCATTGTTTT CATGATTCTC 
CTTAGTAGTG GTGCTCTGGC ATTTGAAGAT ATATATATTG ATCAGCGAAA GACGATTAAG 
ACGATGTTGG AATATGCTGA CAAGGTTTTC ACTTACATTT TCATTCTGGA AATGCTTCTA 
AAATGGGTGG CATATGGCTA TCAAACATAT TTCACCAATG CCTGGTGTTG GCTGGACTTC 
TTAATTGTTG ATGTTTCATT GGTCAGTTTA ACAGCAAATG CCTTGGGTTA CTCAGAACTT 
GGAGCCATCA AATCTCTCAG GACACTAAGA GCTCTGAGAC CTCTAAGAGC CTTATCTCGA 
TTTGAAGGGA TGAGGGTGGT TGTGAATGCC CTTTTAGGAG CAATTCCATC CATCATGAAT 
GTGCTTCTGG TTTGTCTTAT ATTCTGGCTA ATTTTCAGCA TCATGGGCGT AAATTTGTTT 
GCTGGCAAAT TCTACCACTG TATTAACACC ACAACTGGTG ACAGGTTTGA CATCGAAGAC 
GTGAATAATC ATACTGATTG CCTAAAACTA ATAGAAAGAA ATGAGACTGC TCGATGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA TTTGGGTATC TCTCTTTGCT TCAAGTTGCC 
ACATTCAAAG GATGGATGGA TATAATGTAT GCAGCAGTTG ATTCCAGAAA TGTGGAACTC 
CAGCCTAAGT ATGAAGAAAG TCTGTACATG TATCTTTACT TTGTTATTTT CATCATCTTT 
GGGTCCTTCT TCACCTTGAA CCTGTTTATT GGTGTCATCA TAGATAATTT CAACCAGCAG 
AAAAAGAAGT TTGGAGGTCA AGACATCTTT ATGACAGAAG AACAGAAGAA ATACTATAAT 
GCAATGAAAA AATTAGGATC GAAAAAACCG CAAAAGCCTA TACCTCGACC AGGAAACAAA 
TTTCAAGGAA TGGTCTTTGA CTTCGTAACC AGACAAGTTT TTGACATAAG CATCATGATT 
CTCATCTGTC TTAACATGGT CACAATGATG GTGGAAACAG ATGACCAGAG TGAATATGTG 
ACTACCATTT TGTCACGCAT CAATCTGGTG TTCATTGTGC TATTTACTGG AGAGTGTGTA 
CTGAAACTCA TCTCTCTACG CCATTATTAT TTTACCATTG GATGGAATAT TTTTGATTTT 
GTGGTTGTCA TTCTCTCCAT TGTAGGTATG TTTCTTGCCG AGCTGATAGA AAAGTATTTC 
GTGTCCCCTA CCCTGTTCCG AGTGATCCGT CTTGCTAGGA TTGGCCGAAT CCTACGTCTG 
ATCAAAGGAG CAAAGGGGAT CCGCACGCTG CTCTTTGCTT TGATGATGTC CCTTCCTGCG 
TTGTTTAACA TCGGCCTCCT ACTCTTCCTA GTCATGTTCA TCTACGCCAT CTTTGGGATG 
TCCAACTTTG CCTATGTTAA GAGGGAAGTT GGGATCGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CCTATTCCAA ATTACAACCT CTGCTGGCTG GGATGGATTG 
CTAGCACCCA TTCTCAACAG TAAGCCACCC GACTGTGACC CTAATAAAGT TAACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTTGGAA TTTTCTTTTT TGTCAGTTAC 
ATCATCATAT CCTTCCTGGT TGTGGTGAAC ATGTACATCG CGGTCATCCT GGAGAACTTC 
AGTGTTGCTA CTGAAGAAAG TGCAGAGCCT CTGAGTGAGG ATGACTTTGA GATGTTCTAT 
GAGGTTTGGG AGAAGTTTGA TCCCGATGCA ACTCAGTTCA TGGAATTTGA AAAATTATCT 
CAGTTTGCAG CTGCGCTTGA ACCGCCTCTC AATCTGCCAC AACCAAACAA ACTCCAGCTC 
ATTGCCATGG ATTTGCCCAT GGTGAGTGGT GACCGGATCC ACTGTCTTGA TATCTTATTT 
GCTTTTACAA AGCGGGTTCT AGGAGAGAGT GGAGAGATGG ATGCTCTACG AATACAGATG 
GAAGAGCGAT TCATGGCTTC CAATCCTTCC AAGGTCTCCT ATCAGCCAAT CACTACTACT 
TTAAAACGAA AACAAGAGGA AGTATCTGCT GTCATTATTC AGCGTGCTTA CAGACGCCAC 
CTTTTAAAGC GAACTGTAAA ACAAGCTTCC TTTACGTACA ATAAAAACAA AATCAAAGGT 
GGGGCTAATC TTCTTATAAA AGAAGACATG ATAATTGACA GAATAAATGA AAACTCTATT 
ACAGAAAAAA CTGATCTGAC CATGTCCACT GCAGCTTGTC CACCTTCCTA TGACCGGGTG 
ACAAAGCCAA TTGTGGAAAA ACATGAGCAA GAAGGCAAAG ATGAAAAAGC CAAAGGGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860142.1
CDS	400..6342
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017004653.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004653.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGGAA CAACCACTGA AACTGAAATG 
AGAAAGAGAA GGTCAAGTTC TTTCCACGTT TCCATGGACT TTCTAGAAGA TCCTTCCCAA 
AGGCAACGAG CAATGAGTAT AGCCAGCATT CTAACAAATA CAGTAGAAGA ACTTGAAGAA 
TCCAGGCAGA AATGCCCACC CTGTTGGTAT AAATTTTCCA ACATATTCTT AATCTGGGAC 
TGTTCTCCAT ATTGGTTAAA AGTGAAACAT GTTGTCAACC TGGTTGTGAT GGACCCATTT 
GTTGACCTGG CCATCACCAT CTGTATTGTC TTAAATACTC TTTTCATGGC CATGGAGCAC 
TATCCAATGA CGGACCATTT CAATAATGTG CTTACAGTAG GAAACTTGGT TTTCACTGGG 
ATCTTTACAG CAGAAATGTT TCTGAAAATT ATTGCCATGG ATCCTTACTA TTATTTCCAA 
GAAGGCTGGA ATATCTTTGA CGGTTTTATT GTGACGCTTA GCCTGGTAGA ACTTGGACTC 
GCCAATGTGG AAGGATTATC TGTTCTCCGT TCATTTCGAT TGCTGCGAGT TTTCAAGTTG 
GCAAAATCTT GGCCAACGTT AAATATGCTA ATAAAGATCA TCGGCAATTC CGTGGGGGCT 
CTGGGAAATT TAACCCTCGT CTTGGCCATC ATCGTCTTCA TTTTTGCCGT GGTCGGCATG 
CAGCTCTTTG GTAAAAGCTA CAAAGATTGT GTCTGCAAGA TCGCCAGTGA TTGTCAACTC 
CCACGCTGGC ACATGAATGA CTTCTTCCAC TCCTTCCTGA TTGTGTTCCG CGTGCTGTGT 
GGGGAGTGGA TAGAGACCAT GTGGGACTGT ATGGAGGTTG CTGGTCAAGC CATGTGCCTT 
ACTGTCTTCA TGATGGTCAT GGTGATTGGA AACCTAGTGG TCCTGAATCT CTTTCTGGCC 
TTGCTTCTGA GCTCATTTAG TGCAGACAAC CTTGCAGCCA CTGATGATGA TAATGAAATG 
AATAATCTCC AAATTGCTGT GGATAGGATG CACAAAGGAG TAGCTTATGT GAAAAGAAAA 
ATATATGAAT TTATTCAACA GTCCTTCATT AGGAAACAAA AGATTTTAGA TGAAATTAAA 
CCACTTGATG ATCTAAACAA CAAGAAAGAC AGTTGTATGT CCAATCATAC AGCAGAAATT 
GGGAAAGATC TTGACTATCT TAAAGATGTA AATGGAACTA CAAGTGGTAT AGGAACTGGC 
AGCAGTGTTG AAAAATACAT TATTGATGAA AGTGATTACA TGTCATTCAT AAACAACCCC 
AGTCTTACTG TGACTGTACC AATTGCTGTA GGAGAATCTG ACTTTGAAAA TTTAAACACG 
GAAGACTTTA GTAGTGAATC GGATCTGGAA GAAAGCAAAG AGAAACTGAA TGAAAGCAGT 
AGCTCATCAG AAGGTAGCAC TGTGGACATC GGCGCACCTG TAGAAGAACA GCCCGTAGTG 
GAACCTGAAG AAACTCTTGA ACCAGAAGCT TGTTTCACTG AAGGCTGTGT ACAAAGATTC 
AAGTGTTGTC AAATCAATGT GGAAGAAGGC AGAGGAAAAC AATGGTGGAA CCTGAGAAGG 
ACGTGTTTCC GAATAGTTGA ACATAACTGG TTTGAGACCT TCATTGTTTT CATGATTCTC 
CTTAGTAGTG GTGCTCTGGC ATTTGAAGAT ATATATATTG ATCAGCGAAA GACGATTAAG 
ACGATGTTGG AATATGCTGA CAAGGTTTTC ACTTACATTT TCATTCTGGA AATGCTTCTA 
AAATGGGTGG CATATGGCTA TCAAACATAT TTCACCAATG CCTGGTGTTG GCTGGACTTC 
TTAATTGTTG ATGTTTCATT GGTCAGTTTA ACAGCAAATG CCTTGGGTTA CTCAGAACTT 
GGAGCCATCA AATCTCTCAG GACACTAAGA GCTCTGAGAC CTCTAAGAGC CTTATCTCGA 
TTTGAAGGGA TGAGGGTGGT TGTGAATGCC CTTTTAGGAG CAATTCCATC CATCATGAAT 
GTGCTTCTGG TTTGTCTTAT ATTCTGGCTA ATTTTCAGCA TCATGGGCGT AAATTTGTTT 
GCTGGCAAAT TCTACCACTG TATTAACACC ACAACTGGTG ACAGGTTTGA CATCGAAGAC 
GTGAATAATC ATACTGATTG CCTAAAACTA ATAGAAAGAA ATGAGACTGC TCGATGGAAA 
AATGTGAAAG TAAACTTTGA TAATGTAGGA TTTGGGTATC TCTCTTTGCT TCAAGTTGCC 
ACATTCAAAG GATGGATGGA TATAATGTAT GCAGCAGTTG ATTCCAGAAA TGTGGAACTC 
CAGCCTAAGT ATGAAGAAAG TCTGTACATG TATCTTTACT TTGTTATTTT CATCATCTTT 
GGGTCCTTCT TCACCTTGAA CCTGTTTATT GGTGTCATCA TAGATAATTT CAACCAGCAG 
AAAAAGAAGT TTGGAGGTCA AGACATCTTT ATGACAGAAG AACAGAAGAA ATACTATAAT 
GCAATGAAAA AATTAGGATC GAAAAAACCG CAAAAGCCTA TACCTCGACC AGGAAACAAA 
TTTCAAGGAA TGGTCTTTGA CTTCGTAACC AGACAAGTTT TTGACATAAG CATCATGATT 
CTCATCTGTC TTAACATGGT CACAATGATG GTGGAAACAG ATGACCAGAG TGAATATGTG 
ACTACCATTT TGTCACGCAT CAATCTGGTG TTCATTGTGC TATTTACTGG AGAGTGTGTA 
CTGAAACTCA TCTCTCTACG CCATTATTAT TTTACCATTG GATGGAATAT TTTTGATTTT 
GTGGTTGTCA TTCTCTCCAT TGTAGGTATG TTTCTTGCCG AGCTGATAGA AAAGTATTTC 
GTGTCCCCTA CCCTGTTCCG AGTGATCCGT CTTGCTAGGA TTGGCCGAAT CCTACGTCTG 
ATCAAAGGAG CAAAGGGGAT CCGCACGCTG CTCTTTGCTT TGATGATGTC CCTTCCTGCG 
TTGTTTAACA TCGGCCTCCT ACTCTTCCTA GTCATGTTCA TCTACGCCAT CTTTGGGATG 
TCCAACTTTG CCTATGTTAA GAGGGAAGTT GGGATCGATG ACATGTTCAA CTTTGAGACC 
TTTGGCAACA GCATGATCTG CCTATTCCAA ATTACAACCT CTGCTGGCTG GGATGGATTG 
CTAGCACCCA TTCTCAACAG TAAGCCACCC GACTGTGACC CTAATAAAGT TAACCCTGGA 
AGCTCAGTTA AGGGAGACTG TGGGAACCCA TCTGTTGGAA TTTTCTTTTT TGTCAGTTAC 
ATCATCATAT CCTTCCTGGT TGTGGTGAAC ATGTACATCG CGGTCATCCT GGAGAACTTC 
AGTGTTGCTA CTGAAGAAAG TGCAGAGCCT CTGAGTGAGG ATGACTTTGA GATGTTCTAT 
GAGGTTTGGG AGAAGTTTGA TCCCGATGCA ACTCAGTTCA TGGAATTTGA AAAATTATCT 
CAGTTTGCAG CTGCGCTTGA ACCGCCTCTC AATCTGCCAC AACCAAACAA ACTCCAGCTC 
ATTGCCATGG ATTTGCCCAT GGTGAGTGGT GACCGGATCC ACTGTCTTGA TATCTTATTT 
GCTTTTACAA AGCGGGTTCT AGGAGAGAGT GGAGAGATGG ATGCTCTACG AATACAGATG 
GAAGAGCGAT TCATGGCTTC CAATCCTTCC AAGGTCTCCT ATCAGCCAAT CACTACTACT 
TTAAAACGAA AACAAGAGGA AGTATCTGCT GTCATTATTC AGCGTGCTTA CAGACGCCAC 
CTTTTAAAGC GAACTGTAAA ACAAGCTTCC TTTACGTACA ATAAAAACAA AATCAAAGGT 
GGGGCTAATC TTCTTATAAA AGAAGACATG ATAATTGACA GAATAAATGA AAACTCTATT 
ACAGAAAAAA CTGATCTGAC CATGTCCACT GCAGCTTGTC CACCTTCCTA TGACCGGGTG 
ACAAAGCCAA TTGTGGAAAA ACATGAGCAA GAAGGCAAAG ATGAAAAAGC CAAAGGGAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353957.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340886.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 12, mRNA.

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CloneID	OHu23006
Clone ID Related Accession (Same CDS sequence)	NM_001353957.1 , XM_017004651.1 , NM_001165964.3 , NM_001165964.1 , XM_011511606.2 , XM_017004652.1 , NM_001353958.2 , NM_001353958.1 , NM_001165964.2 , NM_001353957.2
Accession Version	NM_001353957.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.102252.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340886.1
CDS	319..6264
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353957.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353957.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001165964.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159436.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 3, mRNA.

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CloneID	OHu23006
Clone ID Related Accession (Same CDS sequence)	NM_001353957.1 , XM_017004651.1 , NM_001165964.3 , NM_001165964.1 , XM_011511606.2 , XM_017004652.1 , NM_001353958.2 , NM_001353958.1 , NM_001165964.2 , NM_001353957.2
Accession Version	NM_001165964.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. On Jun 2, 2019 this sequence version replaced NM_001165964.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.141021.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159436.1
CDS	392..6337
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001165964.3
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165964.3

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001165964.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159436.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 3, mRNA.

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CloneID	OHu23006
Clone ID Related Accession (Same CDS sequence)	NM_001353957.1 , XM_017004651.1 , NM_001165964.3 , NM_001165964.1 , XM_011511606.2 , XM_017004652.1 , NM_001353958.2 , NM_001353958.1 , NM_001165964.2 , NM_001353957.2
Accession Version	NM_001165964.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-08
Organism	Homo sapiens(Human)
Product	sodium channel protein type 1 subunit alpha isoform 3
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12 and AB098335.1. Transcript Variant: This variant (3) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB098335.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159436.1
CDS	19..5964
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001165964.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165964.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353958.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340887.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 13, mRNA.

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CloneID	OHu23006
Clone ID Related Accession (Same CDS sequence)	NM_001353957.1 , XM_017004651.1 , NM_001165964.3 , NM_001165964.1 , XM_011511606.2 , XM_017004652.1 , NM_001353958.2 , NM_001353958.1 , NM_001165964.2 , NM_001353957.2
Accession Version	NM_001353958.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107082.3 and AC010127.12. On Jun 2, 2019 this sequence version replaced NM_001353958.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.185503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340887.1
CDS	257..6202
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353958.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353958.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353958.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340887.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 13, mRNA.

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CloneID	OHu23006
Clone ID Related Accession (Same CDS sequence)	NM_001353957.1 , XM_017004651.1 , NM_001165964.3 , NM_001165964.1 , XM_011511606.2 , XM_017004652.1 , NM_001353958.2 , NM_001353958.1 , NM_001165964.2 , NM_001353957.2
Accession Version	NM_001353958.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107082.3 and AC010127.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.185503.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340887.1
CDS	312..6257
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353958.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353958.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001165964.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159436.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 3, mRNA.

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CloneID	OHu23006
Clone ID Related Accession (Same CDS sequence)	NM_001353957.1 , XM_017004651.1 , NM_001165964.3 , NM_001165964.1 , XM_011511606.2 , XM_017004652.1 , NM_001353958.2 , NM_001353958.1 , NM_001165964.2 , NM_001353957.2
Accession Version	NM_001165964.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. On Aug 2, 2017 this sequence version replaced NM_001165964.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.141021.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159436.1
CDS	411..6356
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001165964.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001165964.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353957.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340886.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 12, mRNA.

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CloneID	OHu23006
Clone ID Related Accession (Same CDS sequence)	NM_001353957.1 , XM_017004651.1 , NM_001165964.3 , NM_001165964.1 , XM_011511606.2 , XM_017004652.1 , NM_001353958.2 , NM_001353958.1 , NM_001165964.2 , NM_001353957.2
Accession Version	NM_001353957.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. On Jun 2, 2019 this sequence version replaced NM_001353957.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.102252.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340886.1
CDS	300..6245
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353957.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353957.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353949.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340878.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 6, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	NM_001353949.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107082.3 and AC010127.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.42326.1, SRR1803614.197394.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340878.1
CDS	220..6216
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353949.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353949.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_006920.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_008851.3 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 2, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	NM_006920.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. On Jun 2, 2019 this sequence version replaced NM_006920.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.132743.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008851.3
CDS	479..6475
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_006920.6
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006920.6

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_006920.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_008851.3 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 2, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	NM_006920.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-06
Organism	Homo sapiens(Human)
Product	sodium channel protein type 1 subunit alpha isoform 2
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF225985.1, AB093549.1 and AC010127.12. This sequence is a reference standard in the RefSeqGene project. On Oct 6, 2006 this sequence version replaced NM_006920.3. Transcript Variant: This variant (2) uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF225985.1, AY043484.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008851.3
CDS	19..6015
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_006920.4
Organism	Homo sapiens(Human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006920.4

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353952.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340881.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 9, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	NM_001353952.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.185051.1, SRR1803615.74439.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158569, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340881.1
CDS	411..6407
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353952.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353952.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353949.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340878.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 6, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	NM_001353949.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107082.3 and AC010127.12. On Jun 2, 2019 this sequence version replaced NM_001353949.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.42326.1, SRR1803614.197394.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340878.1
CDS	165..6161
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353949.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353949.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353952.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340881.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 9, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	NM_001353952.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. On Jun 2, 2019 this sequence version replaced NM_001353952.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.144066.1, SRR1803611.185051.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158569, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340881.1
CDS	392..6388
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353952.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353952.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353951.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340880.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 8, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	NM_001353951.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.244020.1, SRR1803613.187074.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340880.1
CDS	319..6315
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353951.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353951.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353950.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340879.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 7, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	NM_001353950.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107082.3 and AC010127.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.176376.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340879.1
CDS	312..6308
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353950.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353950.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353950.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340879.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 7, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	NM_001353950.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC107082.3 and AC010127.12. On Jun 2, 2019 this sequence version replaced NM_001353950.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.176376.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340879.1
CDS	257..6253
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353950.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353950.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353951.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340880.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 8, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	NM_001353951.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. On Jun 2, 2019 this sequence version replaced NM_001353951.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.244020.1, SRR1803612.116204.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340880.1
CDS	300..6296
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353951.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353951.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_006920.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_008851.3 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 2, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	NM_006920.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. On Aug 2, 2017 this sequence version replaced NM_006920.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.132743.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_008851.3
CDS	498..6494
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_006920.5
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_006920.5

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353954.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340883.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 10, mRNA.

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CloneID	OHu60371
Clone ID Related Accession (Same CDS sequence)	XM_011511605.2 , NM_001353954.1 , NM_001353955.1 , NM_001353954.2 , NM_001353955.2 , XM_017004650.1
Accession Version	NM_001353954.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.18785.1, SRR1803611.271142.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGTTG GTGGACCTTC AGTTCCTACA 
TCGCCTGTTG GACAGCTTCT GCCAGAGGGA ACAACCACTG AAACTGAAAT GAGAAAGAGA 
AGGTCAAGTT CTTTCCACGT TTCCATGGAC TTTCTAGAAG ATCCTTCCCA AAGGCAACGA 
GCAATGAGTA TAGCCAGCAT TCTAACAAAT ACAGTAGAAG AACTTGAAGA ATCCAGGCAG 
AAATGCCCAC CCTGTTGGTA TAAATTTTCC AACATATTCT TAATCTGGGA CTGTTCTCCA 
TATTGGTTAA AAGTGAAACA TGTTGTCAAC CTGGTTGTGA TGGACCCATT TGTTGACCTG 
GCCATCACCA TCTGTATTGT CTTAAATACT CTTTTCATGG CCATGGAGCA CTATCCAATG 
ACGGACCATT TCAATAATGT GCTTACAGTA GGAAACTTGG TTTTCACTGG GATCTTTACA 
GCAGAAATGT TTCTGAAAAT TATTGCCATG GATCCTTACT ATTATTTCCA AGAAGGCTGG 
AATATCTTTG ACGGTTTTAT TGTGACGCTT AGCCTGGTAG AACTTGGACT CGCCAATGTG 
GAAGGATTAT CTGTTCTCCG TTCATTTCGA TTGCTGCGAG TTTTCAAGTT GGCAAAATCT 
TGGCCAACGT TAAATATGCT AATAAAGATC ATCGGCAATT CCGTGGGGGC TCTGGGAAAT 
TTAACCCTCG TCTTGGCCAT CATCGTCTTC ATTTTTGCCG TGGTCGGCAT GCAGCTCTTT 
GGTAAAAGCT ACAAAGATTG TGTCTGCAAG ATCGCCAGTG ATTGTCAACT CCCACGCTGG 
CACATGAATG ACTTCTTCCA CTCCTTCCTG ATTGTGTTCC GCGTGCTGTG TGGGGAGTGG 
ATAGAGACCA TGTGGGACTG TATGGAGGTT GCTGGTCAAG CCATGTGCCT TACTGTCTTC 
ATGATGGTCA TGGTGATTGG AAACCTAGTG GTCCTGAATC TCTTTCTGGC CTTGCTTCTG 
AGCTCATTTA GTGCAGACAA CCTTGCAGCC ACTGATGATG ATAATGAAAT GAATAATCTC 
CAAATTGCTG TGGATAGGAT GCACAAAGGA GTAGCTTATG TGAAAAGAAA AATATATGAA 
TTTATTCAAC AGTCCTTCAT TAGGAAACAA AAGATTTTAG ATGAAATTAA ACCACTTGAT 
GATCTAAACA ACAAGAAAGA CAGTTGTATG TCCAATCATA CAGCAGAAAT TGGGAAAGAT 
CTTGACTATC TTAAAGATGT AAATGGAACT ACAAGTGGTA TAGGAACTGG CAGCAGTGTT 
GAAAAATACA TTATTGATGA AAGTGATTAC ATGTCATTCA TAAACAACCC CAGTCTTACT 
GTGACTGTAC CAATTGCTGT AGGAGAATCT GACTTTGAAA ATTTAAACAC GGAAGACTTT 
AGTAGTGAAT CGGATCTGGA AGAAAGCAAA GAGAAACTGA ATGAAAGCAG TAGCTCATCA 
GAAGGTAGCA CTGTGGACAT CGGCGCACCT GTAGAAGAAC AGCCCGTAGT GGAACCTGAA 
GAAACTCTTG AACCAGAAGC TTGTTTCACT GAAGGCTGTG TACAAAGATT CAAGTGTTGT 
CAAATCAATG TGGAAGAAGG CAGAGGAAAA CAATGGTGGA ACCTGAGAAG GACGTGTTTC 
CGAATAGTTG AACATAACTG GTTTGAGACC TTCATTGTTT TCATGATTCT CCTTAGTAGT 
GGTGCTCTGG CATTTGAAGA TATATATATT GATCAGCGAA AGACGATTAA GACGATGTTG 
GAATATGCTG ACAAGGTTTT CACTTACATT TTCATTCTGG AAATGCTTCT AAAATGGGTG 
GCATATGGCT ATCAAACATA TTTCACCAAT GCCTGGTGTT GGCTGGACTT CTTAATTGTT 
GATGTTTCAT TGGTCAGTTT AACAGCAAAT GCCTTGGGTT ACTCAGAACT TGGAGCCATC 
AAATCTCTCA GGACACTAAG AGCTCTGAGA CCTCTAAGAG CCTTATCTCG ATTTGAAGGG 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA TGTGCTTCTG 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGCG TAAATTTGTT TGCTGGCAAA 
TTCTACCACT GTATTAACAC CACAACTGGT GACAGGTTTG ACATCGAAGA CGTGAATAAT 
CATACTGATT GCCTAAAACT AATAGAAAGA AATGAGACTG CTCGATGGAA AAATGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTCTCTTTGC TTCAAGTTGC CACATTCAAA 
GGATGGATGG ATATAATGTA TGCAGCAGTT GATTCCAGAA ATGTGGAACT CCAGCCTAAG 
TATGAAGAAA GTCTGTACAT GTATCTTTAC TTTGTTATTT TCATCATCTT TGGGTCCTTC 
TTCACCTTGA ACCTGTTTAT TGGTGTCATC ATAGATAATT TCAACCAGCA GAAAAAGAAG 
TTTGGAGGTC AAGACATCTT TATGACAGAA GAACAGAAGA AATACTATAA TGCAATGAAA 
AAATTAGGAT CGAAAAAACC GCAAAAGCCT ATACCTCGAC CAGGAAACAA ATTTCAAGGA 
ATGGTCTTTG ACTTCGTAAC CAGACAAGTT TTTGACATAA GCATCATGAT TCTCATCTGT 
CTTAACATGG TCACAATGAT GGTGGAAACA GATGACCAGA GTGAATATGT GACTACCATT 
TTGTCACGCA TCAATCTGGT GTTCATTGTG CTATTTACTG GAGAGTGTGT ACTGAAACTC 
ATCTCTCTAC GCCATTATTA TTTTACCATT GGATGGAATA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCCA TTGTAGGTAT GTTTCTTGCC GAGCTGATAG AAAAGTATTT CGTGTCCCCT 
ACCCTGTTCC GAGTGATCCG TCTTGCTAGG ATTGGCCGAA TCCTACGTCT GATCAAAGGA 
GCAAAGGGGA TCCGCACGCT GCTCTTTGCT TTGATGATGT CCCTTCCTGC GTTGTTTAAC 
ATCGGCCTCC TACTCTTCCT AGTCATGTTC ATCTACGCCA TCTTTGGGAT GTCCAACTTT 
GCCTATGTTA AGAGGGAAGT TGGGATCGAT GACATGTTCA ACTTTGAGAC CTTTGGCAAC 
AGCATGATCT GCCTATTCCA AATTACAACC TCTGCTGGCT GGGATGGATT GCTAGCACCC 
ATTCTCAACA GTAAGCCACC CGACTGTGAC CCTAATAAAG TTAACCCTGG AAGCTCAGTT 
AAGGGAGACT GTGGGAACCC ATCTGTTGGA ATTTTCTTTT TTGTCAGTTA CATCATCATA 
TCCTTCCTGG TTGTGGTGAA CATGTACATC GCGGTCATCC TGGAGAACTT CAGTGTTGCT 
ACTGAAGAAA GTGCAGAGCC TCTGAGTGAG GATGACTTTG AGATGTTCTA TGAGGTTTGG 
GAGAAGTTTG ATCCCGATGC AACTCAGTTC ATGGAATTTG AAAAATTATC TCAGTTTGCA 
GCTGCGCTTG AACCGCCTCT CAATCTGCCA CAACCAAACA AACTCCAGCT CATTGCCATG 
GATTTGCCCA TGGTGAGTGG TGACCGGATC CACTGTCTTG ATATCTTATT TGCTTTTACA 
AAGCGGGTTC TAGGAGAGAG TGGAGAGATG GATGCTCTAC GAATACAGAT GGAAGAGCGA 
TTCATGGCTT CCAATCCTTC CAAGGTCTCC TATCAGCCAA TCACTACTAC TTTAAAACGA 
AAACAAGAGG AAGTATCTGC TGTCATTATT CAGCGTGCTT ACAGACGCCA CCTTTTAAAG 
CGAACTGTAA AACAAGCTTC CTTTACGTAC AATAAAAACA AAATCAAAGG TGGGGCTAAT 
CTTCTTATAA AAGAAGACAT GATAATTGAC AGAATAAATG AAAACTCTAT TACAGAAAAA 
ACTGATCTGA CCATGTCCAC TGCAGCTTGT CCACCTTCCT ATGACCGGGT GACAAAGCCA 
ATTGTGGAAA AACATGAGCA AGAAGGCAAA GATGAAAAAG CCAAAGGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340883.1
CDS	411..6404
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353954.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353954.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGTTG GTGGACCTTC AGTTCCTACA 
TCGCCTGTTG GACAGCTTCT GCCAGAGGGA ACAACCACTG AAACTGAAAT GAGAAAGAGA 
AGGTCAAGTT CTTTCCACGT TTCCATGGAC TTTCTAGAAG ATCCTTCCCA AAGGCAACGA 
GCAATGAGTA TAGCCAGCAT TCTAACAAAT ACAGTAGAAG AACTTGAAGA ATCCAGGCAG 
AAATGCCCAC CCTGTTGGTA TAAATTTTCC AACATATTCT TAATCTGGGA CTGTTCTCCA 
TATTGGTTAA AAGTGAAACA TGTTGTCAAC CTGGTTGTGA TGGACCCATT TGTTGACCTG 
GCCATCACCA TCTGTATTGT CTTAAATACT CTTTTCATGG CCATGGAGCA CTATCCAATG 
ACGGACCATT TCAATAATGT GCTTACAGTA GGAAACTTGG TTTTCACTGG GATCTTTACA 
GCAGAAATGT TTCTGAAAAT TATTGCCATG GATCCTTACT ATTATTTCCA AGAAGGCTGG 
AATATCTTTG ACGGTTTTAT TGTGACGCTT AGCCTGGTAG AACTTGGACT CGCCAATGTG 
GAAGGATTAT CTGTTCTCCG TTCATTTCGA TTGCTGCGAG TTTTCAAGTT GGCAAAATCT 
TGGCCAACGT TAAATATGCT AATAAAGATC ATCGGCAATT CCGTGGGGGC TCTGGGAAAT 
TTAACCCTCG TCTTGGCCAT CATCGTCTTC ATTTTTGCCG TGGTCGGCAT GCAGCTCTTT 
GGTAAAAGCT ACAAAGATTG TGTCTGCAAG ATCGCCAGTG ATTGTCAACT CCCACGCTGG 
CACATGAATG ACTTCTTCCA CTCCTTCCTG ATTGTGTTCC GCGTGCTGTG TGGGGAGTGG 
ATAGAGACCA TGTGGGACTG TATGGAGGTT GCTGGTCAAG CCATGTGCCT TACTGTCTTC 
ATGATGGTCA TGGTGATTGG AAACCTAGTG GTCCTGAATC TCTTTCTGGC CTTGCTTCTG 
AGCTCATTTA GTGCAGACAA CCTTGCAGCC ACTGATGATG ATAATGAAAT GAATAATCTC 
CAAATTGCTG TGGATAGGAT GCACAAAGGA GTAGCTTATG TGAAAAGAAA AATATATGAA 
TTTATTCAAC AGTCCTTCAT TAGGAAACAA AAGATTTTAG ATGAAATTAA ACCACTTGAT 
GATCTAAACA ACAAGAAAGA CAGTTGTATG TCCAATCATA CAGCAGAAAT TGGGAAAGAT 
CTTGACTATC TTAAAGATGT AAATGGAACT ACAAGTGGTA TAGGAACTGG CAGCAGTGTT 
GAAAAATACA TTATTGATGA AAGTGATTAC ATGTCATTCA TAAACAACCC CAGTCTTACT 
GTGACTGTAC CAATTGCTGT AGGAGAATCT GACTTTGAAA ATTTAAACAC GGAAGACTTT 
AGTAGTGAAT CGGATCTGGA AGAAAGCAAA GAGAAACTGA ATGAAAGCAG TAGCTCATCA 
GAAGGTAGCA CTGTGGACAT CGGCGCACCT GTAGAAGAAC AGCCCGTAGT GGAACCTGAA 
GAAACTCTTG AACCAGAAGC TTGTTTCACT GAAGGCTGTG TACAAAGATT CAAGTGTTGT 
CAAATCAATG TGGAAGAAGG CAGAGGAAAA CAATGGTGGA ACCTGAGAAG GACGTGTTTC 
CGAATAGTTG AACATAACTG GTTTGAGACC TTCATTGTTT TCATGATTCT CCTTAGTAGT 
GGTGCTCTGG CATTTGAAGA TATATATATT GATCAGCGAA AGACGATTAA GACGATGTTG 
GAATATGCTG ACAAGGTTTT CACTTACATT TTCATTCTGG AAATGCTTCT AAAATGGGTG 
GCATATGGCT ATCAAACATA TTTCACCAAT GCCTGGTGTT GGCTGGACTT CTTAATTGTT 
GATGTTTCAT TGGTCAGTTT AACAGCAAAT GCCTTGGGTT ACTCAGAACT TGGAGCCATC 
AAATCTCTCA GGACACTAAG AGCTCTGAGA CCTCTAAGAG CCTTATCTCG ATTTGAAGGG 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA TGTGCTTCTG 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGCG TAAATTTGTT TGCTGGCAAA 
TTCTACCACT GTATTAACAC CACAACTGGT GACAGGTTTG ACATCGAAGA CGTGAATAAT 
CATACTGATT GCCTAAAACT AATAGAAAGA AATGAGACTG CTCGATGGAA AAATGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTCTCTTTGC TTCAAGTTGC CACATTCAAA 
GGATGGATGG ATATAATGTA TGCAGCAGTT GATTCCAGAA ATGTGGAACT CCAGCCTAAG 
TATGAAGAAA GTCTGTACAT GTATCTTTAC TTTGTTATTT TCATCATCTT TGGGTCCTTC 
TTCACCTTGA ACCTGTTTAT TGGTGTCATC ATAGATAATT TCAACCAGCA GAAAAAGAAG 
TTTGGAGGTC AAGACATCTT TATGACAGAA GAACAGAAGA AATACTATAA TGCAATGAAA 
AAATTAGGAT CGAAAAAACC GCAAAAGCCT ATACCTCGAC CAGGAAACAA ATTTCAAGGA 
ATGGTCTTTG ACTTCGTAAC CAGACAAGTT TTTGACATAA GCATCATGAT TCTCATCTGT 
CTTAACATGG TCACAATGAT GGTGGAAACA GATGACCAGA GTGAATATGT GACTACCATT 
TTGTCACGCA TCAATCTGGT GTTCATTGTG CTATTTACTG GAGAGTGTGT ACTGAAACTC 
ATCTCTCTAC GCCATTATTA TTTTACCATT GGATGGAATA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCCA TTGTAGGTAT GTTTCTTGCC GAGCTGATAG AAAAGTATTT CGTGTCCCCT 
ACCCTGTTCC GAGTGATCCG TCTTGCTAGG ATTGGCCGAA TCCTACGTCT GATCAAAGGA 
GCAAAGGGGA TCCGCACGCT GCTCTTTGCT TTGATGATGT CCCTTCCTGC GTTGTTTAAC 
ATCGGCCTCC TACTCTTCCT AGTCATGTTC ATCTACGCCA TCTTTGGGAT GTCCAACTTT 
GCCTATGTTA AGAGGGAAGT TGGGATCGAT GACATGTTCA ACTTTGAGAC CTTTGGCAAC 
AGCATGATCT GCCTATTCCA AATTACAACC TCTGCTGGCT GGGATGGATT GCTAGCACCC 
ATTCTCAACA GTAAGCCACC CGACTGTGAC CCTAATAAAG TTAACCCTGG AAGCTCAGTT 
AAGGGAGACT GTGGGAACCC ATCTGTTGGA ATTTTCTTTT TTGTCAGTTA CATCATCATA 
TCCTTCCTGG TTGTGGTGAA CATGTACATC GCGGTCATCC TGGAGAACTT CAGTGTTGCT 
ACTGAAGAAA GTGCAGAGCC TCTGAGTGAG GATGACTTTG AGATGTTCTA TGAGGTTTGG 
GAGAAGTTTG ATCCCGATGC AACTCAGTTC ATGGAATTTG AAAAATTATC TCAGTTTGCA 
GCTGCGCTTG AACCGCCTCT CAATCTGCCA CAACCAAACA AACTCCAGCT CATTGCCATG 
GATTTGCCCA TGGTGAGTGG TGACCGGATC CACTGTCTTG ATATCTTATT TGCTTTTACA 
AAGCGGGTTC TAGGAGAGAG TGGAGAGATG GATGCTCTAC GAATACAGAT GGAAGAGCGA 
TTCATGGCTT CCAATCCTTC CAAGGTCTCC TATCAGCCAA TCACTACTAC TTTAAAACGA 
AAACAAGAGG AAGTATCTGC TGTCATTATT CAGCGTGCTT ACAGACGCCA CCTTTTAAAG 
CGAACTGTAA AACAAGCTTC CTTTACGTAC AATAAAAACA AAATCAAAGG TGGGGCTAAT 
CTTCTTATAA AAGAAGACAT GATAATTGAC AGAATAAATG AAAACTCTAT TACAGAAAAA 
ACTGATCTGA CCATGTCCAC TGCAGCTTGT CCACCTTCCT ATGACCGGGT GACAAAGCCA 
ATTGTGGAAA AACATGAGCA AGAAGGCAAA GATGAAAAAG CCAAAGGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353955.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340884.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 11, mRNA.

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CloneID	OHu60371
Clone ID Related Accession (Same CDS sequence)	XM_011511605.2 , NM_001353954.1 , NM_001353955.1 , NM_001353954.2 , NM_001353955.2 , XM_017004650.1
Accession Version	NM_001353955.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.163193.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGTTG GTGGACCTTC AGTTCCTACA 
TCGCCTGTTG GACAGCTTCT GCCAGAGGGA ACAACCACTG AAACTGAAAT GAGAAAGAGA 
AGGTCAAGTT CTTTCCACGT TTCCATGGAC TTTCTAGAAG ATCCTTCCCA AAGGCAACGA 
GCAATGAGTA TAGCCAGCAT TCTAACAAAT ACAGTAGAAG AACTTGAAGA ATCCAGGCAG 
AAATGCCCAC CCTGTTGGTA TAAATTTTCC AACATATTCT TAATCTGGGA CTGTTCTCCA 
TATTGGTTAA AAGTGAAACA TGTTGTCAAC CTGGTTGTGA TGGACCCATT TGTTGACCTG 
GCCATCACCA TCTGTATTGT CTTAAATACT CTTTTCATGG CCATGGAGCA CTATCCAATG 
ACGGACCATT TCAATAATGT GCTTACAGTA GGAAACTTGG TTTTCACTGG GATCTTTACA 
GCAGAAATGT TTCTGAAAAT TATTGCCATG GATCCTTACT ATTATTTCCA AGAAGGCTGG 
AATATCTTTG ACGGTTTTAT TGTGACGCTT AGCCTGGTAG AACTTGGACT CGCCAATGTG 
GAAGGATTAT CTGTTCTCCG TTCATTTCGA TTGCTGCGAG TTTTCAAGTT GGCAAAATCT 
TGGCCAACGT TAAATATGCT AATAAAGATC ATCGGCAATT CCGTGGGGGC TCTGGGAAAT 
TTAACCCTCG TCTTGGCCAT CATCGTCTTC ATTTTTGCCG TGGTCGGCAT GCAGCTCTTT 
GGTAAAAGCT ACAAAGATTG TGTCTGCAAG ATCGCCAGTG ATTGTCAACT CCCACGCTGG 
CACATGAATG ACTTCTTCCA CTCCTTCCTG ATTGTGTTCC GCGTGCTGTG TGGGGAGTGG 
ATAGAGACCA TGTGGGACTG TATGGAGGTT GCTGGTCAAG CCATGTGCCT TACTGTCTTC 
ATGATGGTCA TGGTGATTGG AAACCTAGTG GTCCTGAATC TCTTTCTGGC CTTGCTTCTG 
AGCTCATTTA GTGCAGACAA CCTTGCAGCC ACTGATGATG ATAATGAAAT GAATAATCTC 
CAAATTGCTG TGGATAGGAT GCACAAAGGA GTAGCTTATG TGAAAAGAAA AATATATGAA 
TTTATTCAAC AGTCCTTCAT TAGGAAACAA AAGATTTTAG ATGAAATTAA ACCACTTGAT 
GATCTAAACA ACAAGAAAGA CAGTTGTATG TCCAATCATA CAGCAGAAAT TGGGAAAGAT 
CTTGACTATC TTAAAGATGT AAATGGAACT ACAAGTGGTA TAGGAACTGG CAGCAGTGTT 
GAAAAATACA TTATTGATGA AAGTGATTAC ATGTCATTCA TAAACAACCC CAGTCTTACT 
GTGACTGTAC CAATTGCTGT AGGAGAATCT GACTTTGAAA ATTTAAACAC GGAAGACTTT 
AGTAGTGAAT CGGATCTGGA AGAAAGCAAA GAGAAACTGA ATGAAAGCAG TAGCTCATCA 
GAAGGTAGCA CTGTGGACAT CGGCGCACCT GTAGAAGAAC AGCCCGTAGT GGAACCTGAA 
GAAACTCTTG AACCAGAAGC TTGTTTCACT GAAGGCTGTG TACAAAGATT CAAGTGTTGT 
CAAATCAATG TGGAAGAAGG CAGAGGAAAA CAATGGTGGA ACCTGAGAAG GACGTGTTTC 
CGAATAGTTG AACATAACTG GTTTGAGACC TTCATTGTTT TCATGATTCT CCTTAGTAGT 
GGTGCTCTGG CATTTGAAGA TATATATATT GATCAGCGAA AGACGATTAA GACGATGTTG 
GAATATGCTG ACAAGGTTTT CACTTACATT TTCATTCTGG AAATGCTTCT AAAATGGGTG 
GCATATGGCT ATCAAACATA TTTCACCAAT GCCTGGTGTT GGCTGGACTT CTTAATTGTT 
GATGTTTCAT TGGTCAGTTT AACAGCAAAT GCCTTGGGTT ACTCAGAACT TGGAGCCATC 
AAATCTCTCA GGACACTAAG AGCTCTGAGA CCTCTAAGAG CCTTATCTCG ATTTGAAGGG 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA TGTGCTTCTG 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGCG TAAATTTGTT TGCTGGCAAA 
TTCTACCACT GTATTAACAC CACAACTGGT GACAGGTTTG ACATCGAAGA CGTGAATAAT 
CATACTGATT GCCTAAAACT AATAGAAAGA AATGAGACTG CTCGATGGAA AAATGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTCTCTTTGC TTCAAGTTGC CACATTCAAA 
GGATGGATGG ATATAATGTA TGCAGCAGTT GATTCCAGAA ATGTGGAACT CCAGCCTAAG 
TATGAAGAAA GTCTGTACAT GTATCTTTAC TTTGTTATTT TCATCATCTT TGGGTCCTTC 
TTCACCTTGA ACCTGTTTAT TGGTGTCATC ATAGATAATT TCAACCAGCA GAAAAAGAAG 
TTTGGAGGTC AAGACATCTT TATGACAGAA GAACAGAAGA AATACTATAA TGCAATGAAA 
AAATTAGGAT CGAAAAAACC GCAAAAGCCT ATACCTCGAC CAGGAAACAA ATTTCAAGGA 
ATGGTCTTTG ACTTCGTAAC CAGACAAGTT TTTGACATAA GCATCATGAT TCTCATCTGT 
CTTAACATGG TCACAATGAT GGTGGAAACA GATGACCAGA GTGAATATGT GACTACCATT 
TTGTCACGCA TCAATCTGGT GTTCATTGTG CTATTTACTG GAGAGTGTGT ACTGAAACTC 
ATCTCTCTAC GCCATTATTA TTTTACCATT GGATGGAATA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCCA TTGTAGGTAT GTTTCTTGCC GAGCTGATAG AAAAGTATTT CGTGTCCCCT 
ACCCTGTTCC GAGTGATCCG TCTTGCTAGG ATTGGCCGAA TCCTACGTCT GATCAAAGGA 
GCAAAGGGGA TCCGCACGCT GCTCTTTGCT TTGATGATGT CCCTTCCTGC GTTGTTTAAC 
ATCGGCCTCC TACTCTTCCT AGTCATGTTC ATCTACGCCA TCTTTGGGAT GTCCAACTTT 
GCCTATGTTA AGAGGGAAGT TGGGATCGAT GACATGTTCA ACTTTGAGAC CTTTGGCAAC 
AGCATGATCT GCCTATTCCA AATTACAACC TCTGCTGGCT GGGATGGATT GCTAGCACCC 
ATTCTCAACA GTAAGCCACC CGACTGTGAC CCTAATAAAG TTAACCCTGG AAGCTCAGTT 
AAGGGAGACT GTGGGAACCC ATCTGTTGGA ATTTTCTTTT TTGTCAGTTA CATCATCATA 
TCCTTCCTGG TTGTGGTGAA CATGTACATC GCGGTCATCC TGGAGAACTT CAGTGTTGCT 
ACTGAAGAAA GTGCAGAGCC TCTGAGTGAG GATGACTTTG AGATGTTCTA TGAGGTTTGG 
GAGAAGTTTG ATCCCGATGC AACTCAGTTC ATGGAATTTG AAAAATTATC TCAGTTTGCA 
GCTGCGCTTG AACCGCCTCT CAATCTGCCA CAACCAAACA AACTCCAGCT CATTGCCATG 
GATTTGCCCA TGGTGAGTGG TGACCGGATC CACTGTCTTG ATATCTTATT TGCTTTTACA 
AAGCGGGTTC TAGGAGAGAG TGGAGAGATG GATGCTCTAC GAATACAGAT GGAAGAGCGA 
TTCATGGCTT CCAATCCTTC CAAGGTCTCC TATCAGCCAA TCACTACTAC TTTAAAACGA 
AAACAAGAGG AAGTATCTGC TGTCATTATT CAGCGTGCTT ACAGACGCCA CCTTTTAAAG 
CGAACTGTAA AACAAGCTTC CTTTACGTAC AATAAAAACA AAATCAAAGG TGGGGCTAAT 
CTTCTTATAA AAGAAGACAT GATAATTGAC AGAATAAATG AAAACTCTAT TACAGAAAAA 
ACTGATCTGA CCATGTCCAC TGCAGCTTGT CCACCTTCCT ATGACCGGGT GACAAAGCCA 
ATTGTGGAAA AACATGAGCA AGAAGGCAAA GATGAAAAAG CCAAAGGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340884.1
CDS	319..6312
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353955.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353955.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGTTG GTGGACCTTC AGTTCCTACA 
TCGCCTGTTG GACAGCTTCT GCCAGAGGGA ACAACCACTG AAACTGAAAT GAGAAAGAGA 
AGGTCAAGTT CTTTCCACGT TTCCATGGAC TTTCTAGAAG ATCCTTCCCA AAGGCAACGA 
GCAATGAGTA TAGCCAGCAT TCTAACAAAT ACAGTAGAAG AACTTGAAGA ATCCAGGCAG 
AAATGCCCAC CCTGTTGGTA TAAATTTTCC AACATATTCT TAATCTGGGA CTGTTCTCCA 
TATTGGTTAA AAGTGAAACA TGTTGTCAAC CTGGTTGTGA TGGACCCATT TGTTGACCTG 
GCCATCACCA TCTGTATTGT CTTAAATACT CTTTTCATGG CCATGGAGCA CTATCCAATG 
ACGGACCATT TCAATAATGT GCTTACAGTA GGAAACTTGG TTTTCACTGG GATCTTTACA 
GCAGAAATGT TTCTGAAAAT TATTGCCATG GATCCTTACT ATTATTTCCA AGAAGGCTGG 
AATATCTTTG ACGGTTTTAT TGTGACGCTT AGCCTGGTAG AACTTGGACT CGCCAATGTG 
GAAGGATTAT CTGTTCTCCG TTCATTTCGA TTGCTGCGAG TTTTCAAGTT GGCAAAATCT 
TGGCCAACGT TAAATATGCT AATAAAGATC ATCGGCAATT CCGTGGGGGC TCTGGGAAAT 
TTAACCCTCG TCTTGGCCAT CATCGTCTTC ATTTTTGCCG TGGTCGGCAT GCAGCTCTTT 
GGTAAAAGCT ACAAAGATTG TGTCTGCAAG ATCGCCAGTG ATTGTCAACT CCCACGCTGG 
CACATGAATG ACTTCTTCCA CTCCTTCCTG ATTGTGTTCC GCGTGCTGTG TGGGGAGTGG 
ATAGAGACCA TGTGGGACTG TATGGAGGTT GCTGGTCAAG CCATGTGCCT TACTGTCTTC 
ATGATGGTCA TGGTGATTGG AAACCTAGTG GTCCTGAATC TCTTTCTGGC CTTGCTTCTG 
AGCTCATTTA GTGCAGACAA CCTTGCAGCC ACTGATGATG ATAATGAAAT GAATAATCTC 
CAAATTGCTG TGGATAGGAT GCACAAAGGA GTAGCTTATG TGAAAAGAAA AATATATGAA 
TTTATTCAAC AGTCCTTCAT TAGGAAACAA AAGATTTTAG ATGAAATTAA ACCACTTGAT 
GATCTAAACA ACAAGAAAGA CAGTTGTATG TCCAATCATA CAGCAGAAAT TGGGAAAGAT 
CTTGACTATC TTAAAGATGT AAATGGAACT ACAAGTGGTA TAGGAACTGG CAGCAGTGTT 
GAAAAATACA TTATTGATGA AAGTGATTAC ATGTCATTCA TAAACAACCC CAGTCTTACT 
GTGACTGTAC CAATTGCTGT AGGAGAATCT GACTTTGAAA ATTTAAACAC GGAAGACTTT 
AGTAGTGAAT CGGATCTGGA AGAAAGCAAA GAGAAACTGA ATGAAAGCAG TAGCTCATCA 
GAAGGTAGCA CTGTGGACAT CGGCGCACCT GTAGAAGAAC AGCCCGTAGT GGAACCTGAA 
GAAACTCTTG AACCAGAAGC TTGTTTCACT GAAGGCTGTG TACAAAGATT CAAGTGTTGT 
CAAATCAATG TGGAAGAAGG CAGAGGAAAA CAATGGTGGA ACCTGAGAAG GACGTGTTTC 
CGAATAGTTG AACATAACTG GTTTGAGACC TTCATTGTTT TCATGATTCT CCTTAGTAGT 
GGTGCTCTGG CATTTGAAGA TATATATATT GATCAGCGAA AGACGATTAA GACGATGTTG 
GAATATGCTG ACAAGGTTTT CACTTACATT TTCATTCTGG AAATGCTTCT AAAATGGGTG 
GCATATGGCT ATCAAACATA TTTCACCAAT GCCTGGTGTT GGCTGGACTT CTTAATTGTT 
GATGTTTCAT TGGTCAGTTT AACAGCAAAT GCCTTGGGTT ACTCAGAACT TGGAGCCATC 
AAATCTCTCA GGACACTAAG AGCTCTGAGA CCTCTAAGAG CCTTATCTCG ATTTGAAGGG 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA TGTGCTTCTG 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGCG TAAATTTGTT TGCTGGCAAA 
TTCTACCACT GTATTAACAC CACAACTGGT GACAGGTTTG ACATCGAAGA CGTGAATAAT 
CATACTGATT GCCTAAAACT AATAGAAAGA AATGAGACTG CTCGATGGAA AAATGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTCTCTTTGC TTCAAGTTGC CACATTCAAA 
GGATGGATGG ATATAATGTA TGCAGCAGTT GATTCCAGAA ATGTGGAACT CCAGCCTAAG 
TATGAAGAAA GTCTGTACAT GTATCTTTAC TTTGTTATTT TCATCATCTT TGGGTCCTTC 
TTCACCTTGA ACCTGTTTAT TGGTGTCATC ATAGATAATT TCAACCAGCA GAAAAAGAAG 
TTTGGAGGTC AAGACATCTT TATGACAGAA GAACAGAAGA AATACTATAA TGCAATGAAA 
AAATTAGGAT CGAAAAAACC GCAAAAGCCT ATACCTCGAC CAGGAAACAA ATTTCAAGGA 
ATGGTCTTTG ACTTCGTAAC CAGACAAGTT TTTGACATAA GCATCATGAT TCTCATCTGT 
CTTAACATGG TCACAATGAT GGTGGAAACA GATGACCAGA GTGAATATGT GACTACCATT 
TTGTCACGCA TCAATCTGGT GTTCATTGTG CTATTTACTG GAGAGTGTGT ACTGAAACTC 
ATCTCTCTAC GCCATTATTA TTTTACCATT GGATGGAATA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCCA TTGTAGGTAT GTTTCTTGCC GAGCTGATAG AAAAGTATTT CGTGTCCCCT 
ACCCTGTTCC GAGTGATCCG TCTTGCTAGG ATTGGCCGAA TCCTACGTCT GATCAAAGGA 
GCAAAGGGGA TCCGCACGCT GCTCTTTGCT TTGATGATGT CCCTTCCTGC GTTGTTTAAC 
ATCGGCCTCC TACTCTTCCT AGTCATGTTC ATCTACGCCA TCTTTGGGAT GTCCAACTTT 
GCCTATGTTA AGAGGGAAGT TGGGATCGAT GACATGTTCA ACTTTGAGAC CTTTGGCAAC 
AGCATGATCT GCCTATTCCA AATTACAACC TCTGCTGGCT GGGATGGATT GCTAGCACCC 
ATTCTCAACA GTAAGCCACC CGACTGTGAC CCTAATAAAG TTAACCCTGG AAGCTCAGTT 
AAGGGAGACT GTGGGAACCC ATCTGTTGGA ATTTTCTTTT TTGTCAGTTA CATCATCATA 
TCCTTCCTGG TTGTGGTGAA CATGTACATC GCGGTCATCC TGGAGAACTT CAGTGTTGCT 
ACTGAAGAAA GTGCAGAGCC TCTGAGTGAG GATGACTTTG AGATGTTCTA TGAGGTTTGG 
GAGAAGTTTG ATCCCGATGC AACTCAGTTC ATGGAATTTG AAAAATTATC TCAGTTTGCA 
GCTGCGCTTG AACCGCCTCT CAATCTGCCA CAACCAAACA AACTCCAGCT CATTGCCATG 
GATTTGCCCA TGGTGAGTGG TGACCGGATC CACTGTCTTG ATATCTTATT TGCTTTTACA 
AAGCGGGTTC TAGGAGAGAG TGGAGAGATG GATGCTCTAC GAATACAGAT GGAAGAGCGA 
TTCATGGCTT CCAATCCTTC CAAGGTCTCC TATCAGCCAA TCACTACTAC TTTAAAACGA 
AAACAAGAGG AAGTATCTGC TGTCATTATT CAGCGTGCTT ACAGACGCCA CCTTTTAAAG 
CGAACTGTAA AACAAGCTTC CTTTACGTAC AATAAAAACA AAATCAAAGG TGGGGCTAAT 
CTTCTTATAA AAGAAGACAT GATAATTGAC AGAATAAATG AAAACTCTAT TACAGAAAAA 
ACTGATCTGA CCATGTCCAC TGCAGCTTGT CCACCTTCCT ATGACCGGGT GACAAAGCCA 
ATTGTGGAAA AACATGAGCA AGAAGGCAAA GATGAAAAAG CCAAAGGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353954.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340883.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 10, mRNA.

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CloneID	OHu60371
Clone ID Related Accession (Same CDS sequence)	XM_011511605.2 , NM_001353954.1 , NM_001353955.1 , NM_001353954.2 , NM_001353955.2 , XM_017004650.1
Accession Version	NM_001353954.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. On Jun 2, 2019 this sequence version replaced NM_001353954.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.18785.1, SRR1803611.271142.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465402, SAMN03465403 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGTTG GTGGACCTTC AGTTCCTACA 
TCGCCTGTTG GACAGCTTCT GCCAGAGGGA ACAACCACTG AAACTGAAAT GAGAAAGAGA 
AGGTCAAGTT CTTTCCACGT TTCCATGGAC TTTCTAGAAG ATCCTTCCCA AAGGCAACGA 
GCAATGAGTA TAGCCAGCAT TCTAACAAAT ACAGTAGAAG AACTTGAAGA ATCCAGGCAG 
AAATGCCCAC CCTGTTGGTA TAAATTTTCC AACATATTCT TAATCTGGGA CTGTTCTCCA 
TATTGGTTAA AAGTGAAACA TGTTGTCAAC CTGGTTGTGA TGGACCCATT TGTTGACCTG 
GCCATCACCA TCTGTATTGT CTTAAATACT CTTTTCATGG CCATGGAGCA CTATCCAATG 
ACGGACCATT TCAATAATGT GCTTACAGTA GGAAACTTGG TTTTCACTGG GATCTTTACA 
GCAGAAATGT TTCTGAAAAT TATTGCCATG GATCCTTACT ATTATTTCCA AGAAGGCTGG 
AATATCTTTG ACGGTTTTAT TGTGACGCTT AGCCTGGTAG AACTTGGACT CGCCAATGTG 
GAAGGATTAT CTGTTCTCCG TTCATTTCGA TTGCTGCGAG TTTTCAAGTT GGCAAAATCT 
TGGCCAACGT TAAATATGCT AATAAAGATC ATCGGCAATT CCGTGGGGGC TCTGGGAAAT 
TTAACCCTCG TCTTGGCCAT CATCGTCTTC ATTTTTGCCG TGGTCGGCAT GCAGCTCTTT 
GGTAAAAGCT ACAAAGATTG TGTCTGCAAG ATCGCCAGTG ATTGTCAACT CCCACGCTGG 
CACATGAATG ACTTCTTCCA CTCCTTCCTG ATTGTGTTCC GCGTGCTGTG TGGGGAGTGG 
ATAGAGACCA TGTGGGACTG TATGGAGGTT GCTGGTCAAG CCATGTGCCT TACTGTCTTC 
ATGATGGTCA TGGTGATTGG AAACCTAGTG GTCCTGAATC TCTTTCTGGC CTTGCTTCTG 
AGCTCATTTA GTGCAGACAA CCTTGCAGCC ACTGATGATG ATAATGAAAT GAATAATCTC 
CAAATTGCTG TGGATAGGAT GCACAAAGGA GTAGCTTATG TGAAAAGAAA AATATATGAA 
TTTATTCAAC AGTCCTTCAT TAGGAAACAA AAGATTTTAG ATGAAATTAA ACCACTTGAT 
GATCTAAACA ACAAGAAAGA CAGTTGTATG TCCAATCATA CAGCAGAAAT TGGGAAAGAT 
CTTGACTATC TTAAAGATGT AAATGGAACT ACAAGTGGTA TAGGAACTGG CAGCAGTGTT 
GAAAAATACA TTATTGATGA AAGTGATTAC ATGTCATTCA TAAACAACCC CAGTCTTACT 
GTGACTGTAC CAATTGCTGT AGGAGAATCT GACTTTGAAA ATTTAAACAC GGAAGACTTT 
AGTAGTGAAT CGGATCTGGA AGAAAGCAAA GAGAAACTGA ATGAAAGCAG TAGCTCATCA 
GAAGGTAGCA CTGTGGACAT CGGCGCACCT GTAGAAGAAC AGCCCGTAGT GGAACCTGAA 
GAAACTCTTG AACCAGAAGC TTGTTTCACT GAAGGCTGTG TACAAAGATT CAAGTGTTGT 
CAAATCAATG TGGAAGAAGG CAGAGGAAAA CAATGGTGGA ACCTGAGAAG GACGTGTTTC 
CGAATAGTTG AACATAACTG GTTTGAGACC TTCATTGTTT TCATGATTCT CCTTAGTAGT 
GGTGCTCTGG CATTTGAAGA TATATATATT GATCAGCGAA AGACGATTAA GACGATGTTG 
GAATATGCTG ACAAGGTTTT CACTTACATT TTCATTCTGG AAATGCTTCT AAAATGGGTG 
GCATATGGCT ATCAAACATA TTTCACCAAT GCCTGGTGTT GGCTGGACTT CTTAATTGTT 
GATGTTTCAT TGGTCAGTTT AACAGCAAAT GCCTTGGGTT ACTCAGAACT TGGAGCCATC 
AAATCTCTCA GGACACTAAG AGCTCTGAGA CCTCTAAGAG CCTTATCTCG ATTTGAAGGG 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA TGTGCTTCTG 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGCG TAAATTTGTT TGCTGGCAAA 
TTCTACCACT GTATTAACAC CACAACTGGT GACAGGTTTG ACATCGAAGA CGTGAATAAT 
CATACTGATT GCCTAAAACT AATAGAAAGA AATGAGACTG CTCGATGGAA AAATGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTCTCTTTGC TTCAAGTTGC CACATTCAAA 
GGATGGATGG ATATAATGTA TGCAGCAGTT GATTCCAGAA ATGTGGAACT CCAGCCTAAG 
TATGAAGAAA GTCTGTACAT GTATCTTTAC TTTGTTATTT TCATCATCTT TGGGTCCTTC 
TTCACCTTGA ACCTGTTTAT TGGTGTCATC ATAGATAATT TCAACCAGCA GAAAAAGAAG 
TTTGGAGGTC AAGACATCTT TATGACAGAA GAACAGAAGA AATACTATAA TGCAATGAAA 
AAATTAGGAT CGAAAAAACC GCAAAAGCCT ATACCTCGAC CAGGAAACAA ATTTCAAGGA 
ATGGTCTTTG ACTTCGTAAC CAGACAAGTT TTTGACATAA GCATCATGAT TCTCATCTGT 
CTTAACATGG TCACAATGAT GGTGGAAACA GATGACCAGA GTGAATATGT GACTACCATT 
TTGTCACGCA TCAATCTGGT GTTCATTGTG CTATTTACTG GAGAGTGTGT ACTGAAACTC 
ATCTCTCTAC GCCATTATTA TTTTACCATT GGATGGAATA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCCA TTGTAGGTAT GTTTCTTGCC GAGCTGATAG AAAAGTATTT CGTGTCCCCT 
ACCCTGTTCC GAGTGATCCG TCTTGCTAGG ATTGGCCGAA TCCTACGTCT GATCAAAGGA 
GCAAAGGGGA TCCGCACGCT GCTCTTTGCT TTGATGATGT CCCTTCCTGC GTTGTTTAAC 
ATCGGCCTCC TACTCTTCCT AGTCATGTTC ATCTACGCCA TCTTTGGGAT GTCCAACTTT 
GCCTATGTTA AGAGGGAAGT TGGGATCGAT GACATGTTCA ACTTTGAGAC CTTTGGCAAC 
AGCATGATCT GCCTATTCCA AATTACAACC TCTGCTGGCT GGGATGGATT GCTAGCACCC 
ATTCTCAACA GTAAGCCACC CGACTGTGAC CCTAATAAAG TTAACCCTGG AAGCTCAGTT 
AAGGGAGACT GTGGGAACCC ATCTGTTGGA ATTTTCTTTT TTGTCAGTTA CATCATCATA 
TCCTTCCTGG TTGTGGTGAA CATGTACATC GCGGTCATCC TGGAGAACTT CAGTGTTGCT 
ACTGAAGAAA GTGCAGAGCC TCTGAGTGAG GATGACTTTG AGATGTTCTA TGAGGTTTGG 
GAGAAGTTTG ATCCCGATGC AACTCAGTTC ATGGAATTTG AAAAATTATC TCAGTTTGCA 
GCTGCGCTTG AACCGCCTCT CAATCTGCCA CAACCAAACA AACTCCAGCT CATTGCCATG 
GATTTGCCCA TGGTGAGTGG TGACCGGATC CACTGTCTTG ATATCTTATT TGCTTTTACA 
AAGCGGGTTC TAGGAGAGAG TGGAGAGATG GATGCTCTAC GAATACAGAT GGAAGAGCGA 
TTCATGGCTT CCAATCCTTC CAAGGTCTCC TATCAGCCAA TCACTACTAC TTTAAAACGA 
AAACAAGAGG AAGTATCTGC TGTCATTATT CAGCGTGCTT ACAGACGCCA CCTTTTAAAG 
CGAACTGTAA AACAAGCTTC CTTTACGTAC AATAAAAACA AAATCAAAGG TGGGGCTAAT 
CTTCTTATAA AAGAAGACAT GATAATTGAC AGAATAAATG AAAACTCTAT TACAGAAAAA 
ACTGATCTGA CCATGTCCAC TGCAGCTTGT CCACCTTCCT ATGACCGGGT GACAAAGCCA 
ATTGTGGAAA AACATGAGCA AGAAGGCAAA GATGAAAAAG CCAAAGGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340883.1
CDS	392..6385
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353954.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353954.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGTTG GTGGACCTTC AGTTCCTACA 
TCGCCTGTTG GACAGCTTCT GCCAGAGGGA ACAACCACTG AAACTGAAAT GAGAAAGAGA 
AGGTCAAGTT CTTTCCACGT TTCCATGGAC TTTCTAGAAG ATCCTTCCCA AAGGCAACGA 
GCAATGAGTA TAGCCAGCAT TCTAACAAAT ACAGTAGAAG AACTTGAAGA ATCCAGGCAG 
AAATGCCCAC CCTGTTGGTA TAAATTTTCC AACATATTCT TAATCTGGGA CTGTTCTCCA 
TATTGGTTAA AAGTGAAACA TGTTGTCAAC CTGGTTGTGA TGGACCCATT TGTTGACCTG 
GCCATCACCA TCTGTATTGT CTTAAATACT CTTTTCATGG CCATGGAGCA CTATCCAATG 
ACGGACCATT TCAATAATGT GCTTACAGTA GGAAACTTGG TTTTCACTGG GATCTTTACA 
GCAGAAATGT TTCTGAAAAT TATTGCCATG GATCCTTACT ATTATTTCCA AGAAGGCTGG 
AATATCTTTG ACGGTTTTAT TGTGACGCTT AGCCTGGTAG AACTTGGACT CGCCAATGTG 
GAAGGATTAT CTGTTCTCCG TTCATTTCGA TTGCTGCGAG TTTTCAAGTT GGCAAAATCT 
TGGCCAACGT TAAATATGCT AATAAAGATC ATCGGCAATT CCGTGGGGGC TCTGGGAAAT 
TTAACCCTCG TCTTGGCCAT CATCGTCTTC ATTTTTGCCG TGGTCGGCAT GCAGCTCTTT 
GGTAAAAGCT ACAAAGATTG TGTCTGCAAG ATCGCCAGTG ATTGTCAACT CCCACGCTGG 
CACATGAATG ACTTCTTCCA CTCCTTCCTG ATTGTGTTCC GCGTGCTGTG TGGGGAGTGG 
ATAGAGACCA TGTGGGACTG TATGGAGGTT GCTGGTCAAG CCATGTGCCT TACTGTCTTC 
ATGATGGTCA TGGTGATTGG AAACCTAGTG GTCCTGAATC TCTTTCTGGC CTTGCTTCTG 
AGCTCATTTA GTGCAGACAA CCTTGCAGCC ACTGATGATG ATAATGAAAT GAATAATCTC 
CAAATTGCTG TGGATAGGAT GCACAAAGGA GTAGCTTATG TGAAAAGAAA AATATATGAA 
TTTATTCAAC AGTCCTTCAT TAGGAAACAA AAGATTTTAG ATGAAATTAA ACCACTTGAT 
GATCTAAACA ACAAGAAAGA CAGTTGTATG TCCAATCATA CAGCAGAAAT TGGGAAAGAT 
CTTGACTATC TTAAAGATGT AAATGGAACT ACAAGTGGTA TAGGAACTGG CAGCAGTGTT 
GAAAAATACA TTATTGATGA AAGTGATTAC ATGTCATTCA TAAACAACCC CAGTCTTACT 
GTGACTGTAC CAATTGCTGT AGGAGAATCT GACTTTGAAA ATTTAAACAC GGAAGACTTT 
AGTAGTGAAT CGGATCTGGA AGAAAGCAAA GAGAAACTGA ATGAAAGCAG TAGCTCATCA 
GAAGGTAGCA CTGTGGACAT CGGCGCACCT GTAGAAGAAC AGCCCGTAGT GGAACCTGAA 
GAAACTCTTG AACCAGAAGC TTGTTTCACT GAAGGCTGTG TACAAAGATT CAAGTGTTGT 
CAAATCAATG TGGAAGAAGG CAGAGGAAAA CAATGGTGGA ACCTGAGAAG GACGTGTTTC 
CGAATAGTTG AACATAACTG GTTTGAGACC TTCATTGTTT TCATGATTCT CCTTAGTAGT 
GGTGCTCTGG CATTTGAAGA TATATATATT GATCAGCGAA AGACGATTAA GACGATGTTG 
GAATATGCTG ACAAGGTTTT CACTTACATT TTCATTCTGG AAATGCTTCT AAAATGGGTG 
GCATATGGCT ATCAAACATA TTTCACCAAT GCCTGGTGTT GGCTGGACTT CTTAATTGTT 
GATGTTTCAT TGGTCAGTTT AACAGCAAAT GCCTTGGGTT ACTCAGAACT TGGAGCCATC 
AAATCTCTCA GGACACTAAG AGCTCTGAGA CCTCTAAGAG CCTTATCTCG ATTTGAAGGG 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA TGTGCTTCTG 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGCG TAAATTTGTT TGCTGGCAAA 
TTCTACCACT GTATTAACAC CACAACTGGT GACAGGTTTG ACATCGAAGA CGTGAATAAT 
CATACTGATT GCCTAAAACT AATAGAAAGA AATGAGACTG CTCGATGGAA AAATGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTCTCTTTGC TTCAAGTTGC CACATTCAAA 
GGATGGATGG ATATAATGTA TGCAGCAGTT GATTCCAGAA ATGTGGAACT CCAGCCTAAG 
TATGAAGAAA GTCTGTACAT GTATCTTTAC TTTGTTATTT TCATCATCTT TGGGTCCTTC 
TTCACCTTGA ACCTGTTTAT TGGTGTCATC ATAGATAATT TCAACCAGCA GAAAAAGAAG 
TTTGGAGGTC AAGACATCTT TATGACAGAA GAACAGAAGA AATACTATAA TGCAATGAAA 
AAATTAGGAT CGAAAAAACC GCAAAAGCCT ATACCTCGAC CAGGAAACAA ATTTCAAGGA 
ATGGTCTTTG ACTTCGTAAC CAGACAAGTT TTTGACATAA GCATCATGAT TCTCATCTGT 
CTTAACATGG TCACAATGAT GGTGGAAACA GATGACCAGA GTGAATATGT GACTACCATT 
TTGTCACGCA TCAATCTGGT GTTCATTGTG CTATTTACTG GAGAGTGTGT ACTGAAACTC 
ATCTCTCTAC GCCATTATTA TTTTACCATT GGATGGAATA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCCA TTGTAGGTAT GTTTCTTGCC GAGCTGATAG AAAAGTATTT CGTGTCCCCT 
ACCCTGTTCC GAGTGATCCG TCTTGCTAGG ATTGGCCGAA TCCTACGTCT GATCAAAGGA 
GCAAAGGGGA TCCGCACGCT GCTCTTTGCT TTGATGATGT CCCTTCCTGC GTTGTTTAAC 
ATCGGCCTCC TACTCTTCCT AGTCATGTTC ATCTACGCCA TCTTTGGGAT GTCCAACTTT 
GCCTATGTTA AGAGGGAAGT TGGGATCGAT GACATGTTCA ACTTTGAGAC CTTTGGCAAC 
AGCATGATCT GCCTATTCCA AATTACAACC TCTGCTGGCT GGGATGGATT GCTAGCACCC 
ATTCTCAACA GTAAGCCACC CGACTGTGAC CCTAATAAAG TTAACCCTGG AAGCTCAGTT 
AAGGGAGACT GTGGGAACCC ATCTGTTGGA ATTTTCTTTT TTGTCAGTTA CATCATCATA 
TCCTTCCTGG TTGTGGTGAA CATGTACATC GCGGTCATCC TGGAGAACTT CAGTGTTGCT 
ACTGAAGAAA GTGCAGAGCC TCTGAGTGAG GATGACTTTG AGATGTTCTA TGAGGTTTGG 
GAGAAGTTTG ATCCCGATGC AACTCAGTTC ATGGAATTTG AAAAATTATC TCAGTTTGCA 
GCTGCGCTTG AACCGCCTCT CAATCTGCCA CAACCAAACA AACTCCAGCT CATTGCCATG 
GATTTGCCCA TGGTGAGTGG TGACCGGATC CACTGTCTTG ATATCTTATT TGCTTTTACA 
AAGCGGGTTC TAGGAGAGAG TGGAGAGATG GATGCTCTAC GAATACAGAT GGAAGAGCGA 
TTCATGGCTT CCAATCCTTC CAAGGTCTCC TATCAGCCAA TCACTACTAC TTTAAAACGA 
AAACAAGAGG AAGTATCTGC TGTCATTATT CAGCGTGCTT ACAGACGCCA CCTTTTAAAG 
CGAACTGTAA AACAAGCTTC CTTTACGTAC AATAAAAACA AAATCAAAGG TGGGGCTAAT 
CTTCTTATAA AAGAAGACAT GATAATTGAC AGAATAAATG AAAACTCTAT TACAGAAAAA 
ACTGATCTGA CCATGTCCAC TGCAGCTTGT CCACCTTCCT ATGACCGGGT GACAAAGCCA 
ATTGTGGAAA AACATGAGCA AGAAGGCAAA GATGAAAAAG CCAAAGGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( NM_001353955.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001340884.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 11, mRNA.

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CloneID	OHu60371
Clone ID Related Accession (Same CDS sequence)	XM_011511605.2 , NM_001353954.1 , NM_001353955.1 , NM_001353954.2 , NM_001353955.2 , XM_017004650.1
Accession Version	NM_001353955.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC010127.12. On Jun 2, 2019 this sequence version replaced NM_001353955.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.163193.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGTTG GTGGACCTTC AGTTCCTACA 
TCGCCTGTTG GACAGCTTCT GCCAGAGGGA ACAACCACTG AAACTGAAAT GAGAAAGAGA 
AGGTCAAGTT CTTTCCACGT TTCCATGGAC TTTCTAGAAG ATCCTTCCCA AAGGCAACGA 
GCAATGAGTA TAGCCAGCAT TCTAACAAAT ACAGTAGAAG AACTTGAAGA ATCCAGGCAG 
AAATGCCCAC CCTGTTGGTA TAAATTTTCC AACATATTCT TAATCTGGGA CTGTTCTCCA 
TATTGGTTAA AAGTGAAACA TGTTGTCAAC CTGGTTGTGA TGGACCCATT TGTTGACCTG 
GCCATCACCA TCTGTATTGT CTTAAATACT CTTTTCATGG CCATGGAGCA CTATCCAATG 
ACGGACCATT TCAATAATGT GCTTACAGTA GGAAACTTGG TTTTCACTGG GATCTTTACA 
GCAGAAATGT TTCTGAAAAT TATTGCCATG GATCCTTACT ATTATTTCCA AGAAGGCTGG 
AATATCTTTG ACGGTTTTAT TGTGACGCTT AGCCTGGTAG AACTTGGACT CGCCAATGTG 
GAAGGATTAT CTGTTCTCCG TTCATTTCGA TTGCTGCGAG TTTTCAAGTT GGCAAAATCT 
TGGCCAACGT TAAATATGCT AATAAAGATC ATCGGCAATT CCGTGGGGGC TCTGGGAAAT 
TTAACCCTCG TCTTGGCCAT CATCGTCTTC ATTTTTGCCG TGGTCGGCAT GCAGCTCTTT 
GGTAAAAGCT ACAAAGATTG TGTCTGCAAG ATCGCCAGTG ATTGTCAACT CCCACGCTGG 
CACATGAATG ACTTCTTCCA CTCCTTCCTG ATTGTGTTCC GCGTGCTGTG TGGGGAGTGG 
ATAGAGACCA TGTGGGACTG TATGGAGGTT GCTGGTCAAG CCATGTGCCT TACTGTCTTC 
ATGATGGTCA TGGTGATTGG AAACCTAGTG GTCCTGAATC TCTTTCTGGC CTTGCTTCTG 
AGCTCATTTA GTGCAGACAA CCTTGCAGCC ACTGATGATG ATAATGAAAT GAATAATCTC 
CAAATTGCTG TGGATAGGAT GCACAAAGGA GTAGCTTATG TGAAAAGAAA AATATATGAA 
TTTATTCAAC AGTCCTTCAT TAGGAAACAA AAGATTTTAG ATGAAATTAA ACCACTTGAT 
GATCTAAACA ACAAGAAAGA CAGTTGTATG TCCAATCATA CAGCAGAAAT TGGGAAAGAT 
CTTGACTATC TTAAAGATGT AAATGGAACT ACAAGTGGTA TAGGAACTGG CAGCAGTGTT 
GAAAAATACA TTATTGATGA AAGTGATTAC ATGTCATTCA TAAACAACCC CAGTCTTACT 
GTGACTGTAC CAATTGCTGT AGGAGAATCT GACTTTGAAA ATTTAAACAC GGAAGACTTT 
AGTAGTGAAT CGGATCTGGA AGAAAGCAAA GAGAAACTGA ATGAAAGCAG TAGCTCATCA 
GAAGGTAGCA CTGTGGACAT CGGCGCACCT GTAGAAGAAC AGCCCGTAGT GGAACCTGAA 
GAAACTCTTG AACCAGAAGC TTGTTTCACT GAAGGCTGTG TACAAAGATT CAAGTGTTGT 
CAAATCAATG TGGAAGAAGG CAGAGGAAAA CAATGGTGGA ACCTGAGAAG GACGTGTTTC 
CGAATAGTTG AACATAACTG GTTTGAGACC TTCATTGTTT TCATGATTCT CCTTAGTAGT 
GGTGCTCTGG CATTTGAAGA TATATATATT GATCAGCGAA AGACGATTAA GACGATGTTG 
GAATATGCTG ACAAGGTTTT CACTTACATT TTCATTCTGG AAATGCTTCT AAAATGGGTG 
GCATATGGCT ATCAAACATA TTTCACCAAT GCCTGGTGTT GGCTGGACTT CTTAATTGTT 
GATGTTTCAT TGGTCAGTTT AACAGCAAAT GCCTTGGGTT ACTCAGAACT TGGAGCCATC 
AAATCTCTCA GGACACTAAG AGCTCTGAGA CCTCTAAGAG CCTTATCTCG ATTTGAAGGG 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA TGTGCTTCTG 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGCG TAAATTTGTT TGCTGGCAAA 
TTCTACCACT GTATTAACAC CACAACTGGT GACAGGTTTG ACATCGAAGA CGTGAATAAT 
CATACTGATT GCCTAAAACT AATAGAAAGA AATGAGACTG CTCGATGGAA AAATGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTCTCTTTGC TTCAAGTTGC CACATTCAAA 
GGATGGATGG ATATAATGTA TGCAGCAGTT GATTCCAGAA ATGTGGAACT CCAGCCTAAG 
TATGAAGAAA GTCTGTACAT GTATCTTTAC TTTGTTATTT TCATCATCTT TGGGTCCTTC 
TTCACCTTGA ACCTGTTTAT TGGTGTCATC ATAGATAATT TCAACCAGCA GAAAAAGAAG 
TTTGGAGGTC AAGACATCTT TATGACAGAA GAACAGAAGA AATACTATAA TGCAATGAAA 
AAATTAGGAT CGAAAAAACC GCAAAAGCCT ATACCTCGAC CAGGAAACAA ATTTCAAGGA 
ATGGTCTTTG ACTTCGTAAC CAGACAAGTT TTTGACATAA GCATCATGAT TCTCATCTGT 
CTTAACATGG TCACAATGAT GGTGGAAACA GATGACCAGA GTGAATATGT GACTACCATT 
TTGTCACGCA TCAATCTGGT GTTCATTGTG CTATTTACTG GAGAGTGTGT ACTGAAACTC 
ATCTCTCTAC GCCATTATTA TTTTACCATT GGATGGAATA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCCA TTGTAGGTAT GTTTCTTGCC GAGCTGATAG AAAAGTATTT CGTGTCCCCT 
ACCCTGTTCC GAGTGATCCG TCTTGCTAGG ATTGGCCGAA TCCTACGTCT GATCAAAGGA 
GCAAAGGGGA TCCGCACGCT GCTCTTTGCT TTGATGATGT CCCTTCCTGC GTTGTTTAAC 
ATCGGCCTCC TACTCTTCCT AGTCATGTTC ATCTACGCCA TCTTTGGGAT GTCCAACTTT 
GCCTATGTTA AGAGGGAAGT TGGGATCGAT GACATGTTCA ACTTTGAGAC CTTTGGCAAC 
AGCATGATCT GCCTATTCCA AATTACAACC TCTGCTGGCT GGGATGGATT GCTAGCACCC 
ATTCTCAACA GTAAGCCACC CGACTGTGAC CCTAATAAAG TTAACCCTGG AAGCTCAGTT 
AAGGGAGACT GTGGGAACCC ATCTGTTGGA ATTTTCTTTT TTGTCAGTTA CATCATCATA 
TCCTTCCTGG TTGTGGTGAA CATGTACATC GCGGTCATCC TGGAGAACTT CAGTGTTGCT 
ACTGAAGAAA GTGCAGAGCC TCTGAGTGAG GATGACTTTG AGATGTTCTA TGAGGTTTGG 
GAGAAGTTTG ATCCCGATGC AACTCAGTTC ATGGAATTTG AAAAATTATC TCAGTTTGCA 
GCTGCGCTTG AACCGCCTCT CAATCTGCCA CAACCAAACA AACTCCAGCT CATTGCCATG 
GATTTGCCCA TGGTGAGTGG TGACCGGATC CACTGTCTTG ATATCTTATT TGCTTTTACA 
AAGCGGGTTC TAGGAGAGAG TGGAGAGATG GATGCTCTAC GAATACAGAT GGAAGAGCGA 
TTCATGGCTT CCAATCCTTC CAAGGTCTCC TATCAGCCAA TCACTACTAC TTTAAAACGA 
AAACAAGAGG AAGTATCTGC TGTCATTATT CAGCGTGCTT ACAGACGCCA CCTTTTAAAG 
CGAACTGTAA AACAAGCTTC CTTTACGTAC AATAAAAACA AAATCAAAGG TGGGGCTAAT 
CTTCTTATAA AAGAAGACAT GATAATTGAC AGAATAAATG AAAACTCTAT TACAGAAAAA 
ACTGATCTGA CCATGTCCAC TGCAGCTTGT CCACCTTCCT ATGACCGGGT GACAAAGCCA 
ATTGTGGAAA AACATGAGCA AGAAGGCAAA GATGAAAAAG CCAAAGGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001340884.1
CDS	300..6293
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	NM_001353955.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001353955.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGTTG GTGGACCTTC AGTTCCTACA 
TCGCCTGTTG GACAGCTTCT GCCAGAGGGA ACAACCACTG AAACTGAAAT GAGAAAGAGA 
AGGTCAAGTT CTTTCCACGT TTCCATGGAC TTTCTAGAAG ATCCTTCCCA AAGGCAACGA 
GCAATGAGTA TAGCCAGCAT TCTAACAAAT ACAGTAGAAG AACTTGAAGA ATCCAGGCAG 
AAATGCCCAC CCTGTTGGTA TAAATTTTCC AACATATTCT TAATCTGGGA CTGTTCTCCA 
TATTGGTTAA AAGTGAAACA TGTTGTCAAC CTGGTTGTGA TGGACCCATT TGTTGACCTG 
GCCATCACCA TCTGTATTGT CTTAAATACT CTTTTCATGG CCATGGAGCA CTATCCAATG 
ACGGACCATT TCAATAATGT GCTTACAGTA GGAAACTTGG TTTTCACTGG GATCTTTACA 
GCAGAAATGT TTCTGAAAAT TATTGCCATG GATCCTTACT ATTATTTCCA AGAAGGCTGG 
AATATCTTTG ACGGTTTTAT TGTGACGCTT AGCCTGGTAG AACTTGGACT CGCCAATGTG 
GAAGGATTAT CTGTTCTCCG TTCATTTCGA TTGCTGCGAG TTTTCAAGTT GGCAAAATCT 
TGGCCAACGT TAAATATGCT AATAAAGATC ATCGGCAATT CCGTGGGGGC TCTGGGAAAT 
TTAACCCTCG TCTTGGCCAT CATCGTCTTC ATTTTTGCCG TGGTCGGCAT GCAGCTCTTT 
GGTAAAAGCT ACAAAGATTG TGTCTGCAAG ATCGCCAGTG ATTGTCAACT CCCACGCTGG 
CACATGAATG ACTTCTTCCA CTCCTTCCTG ATTGTGTTCC GCGTGCTGTG TGGGGAGTGG 
ATAGAGACCA TGTGGGACTG TATGGAGGTT GCTGGTCAAG CCATGTGCCT TACTGTCTTC 
ATGATGGTCA TGGTGATTGG AAACCTAGTG GTCCTGAATC TCTTTCTGGC CTTGCTTCTG 
AGCTCATTTA GTGCAGACAA CCTTGCAGCC ACTGATGATG ATAATGAAAT GAATAATCTC 
CAAATTGCTG TGGATAGGAT GCACAAAGGA GTAGCTTATG TGAAAAGAAA AATATATGAA 
TTTATTCAAC AGTCCTTCAT TAGGAAACAA AAGATTTTAG ATGAAATTAA ACCACTTGAT 
GATCTAAACA ACAAGAAAGA CAGTTGTATG TCCAATCATA CAGCAGAAAT TGGGAAAGAT 
CTTGACTATC TTAAAGATGT AAATGGAACT ACAAGTGGTA TAGGAACTGG CAGCAGTGTT 
GAAAAATACA TTATTGATGA AAGTGATTAC ATGTCATTCA TAAACAACCC CAGTCTTACT 
GTGACTGTAC CAATTGCTGT AGGAGAATCT GACTTTGAAA ATTTAAACAC GGAAGACTTT 
AGTAGTGAAT CGGATCTGGA AGAAAGCAAA GAGAAACTGA ATGAAAGCAG TAGCTCATCA 
GAAGGTAGCA CTGTGGACAT CGGCGCACCT GTAGAAGAAC AGCCCGTAGT GGAACCTGAA 
GAAACTCTTG AACCAGAAGC TTGTTTCACT GAAGGCTGTG TACAAAGATT CAAGTGTTGT 
CAAATCAATG TGGAAGAAGG CAGAGGAAAA CAATGGTGGA ACCTGAGAAG GACGTGTTTC 
CGAATAGTTG AACATAACTG GTTTGAGACC TTCATTGTTT TCATGATTCT CCTTAGTAGT 
GGTGCTCTGG CATTTGAAGA TATATATATT GATCAGCGAA AGACGATTAA GACGATGTTG 
GAATATGCTG ACAAGGTTTT CACTTACATT TTCATTCTGG AAATGCTTCT AAAATGGGTG 
GCATATGGCT ATCAAACATA TTTCACCAAT GCCTGGTGTT GGCTGGACTT CTTAATTGTT 
GATGTTTCAT TGGTCAGTTT AACAGCAAAT GCCTTGGGTT ACTCAGAACT TGGAGCCATC 
AAATCTCTCA GGACACTAAG AGCTCTGAGA CCTCTAAGAG CCTTATCTCG ATTTGAAGGG 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA TGTGCTTCTG 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGCG TAAATTTGTT TGCTGGCAAA 
TTCTACCACT GTATTAACAC CACAACTGGT GACAGGTTTG ACATCGAAGA CGTGAATAAT 
CATACTGATT GCCTAAAACT AATAGAAAGA AATGAGACTG CTCGATGGAA AAATGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTCTCTTTGC TTCAAGTTGC CACATTCAAA 
GGATGGATGG ATATAATGTA TGCAGCAGTT GATTCCAGAA ATGTGGAACT CCAGCCTAAG 
TATGAAGAAA GTCTGTACAT GTATCTTTAC TTTGTTATTT TCATCATCTT TGGGTCCTTC 
TTCACCTTGA ACCTGTTTAT TGGTGTCATC ATAGATAATT TCAACCAGCA GAAAAAGAAG 
TTTGGAGGTC AAGACATCTT TATGACAGAA GAACAGAAGA AATACTATAA TGCAATGAAA 
AAATTAGGAT CGAAAAAACC GCAAAAGCCT ATACCTCGAC CAGGAAACAA ATTTCAAGGA 
ATGGTCTTTG ACTTCGTAAC CAGACAAGTT TTTGACATAA GCATCATGAT TCTCATCTGT 
CTTAACATGG TCACAATGAT GGTGGAAACA GATGACCAGA GTGAATATGT GACTACCATT 
TTGTCACGCA TCAATCTGGT GTTCATTGTG CTATTTACTG GAGAGTGTGT ACTGAAACTC 
ATCTCTCTAC GCCATTATTA TTTTACCATT GGATGGAATA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCCA TTGTAGGTAT GTTTCTTGCC GAGCTGATAG AAAAGTATTT CGTGTCCCCT 
ACCCTGTTCC GAGTGATCCG TCTTGCTAGG ATTGGCCGAA TCCTACGTCT GATCAAAGGA 
GCAAAGGGGA TCCGCACGCT GCTCTTTGCT TTGATGATGT CCCTTCCTGC GTTGTTTAAC 
ATCGGCCTCC TACTCTTCCT AGTCATGTTC ATCTACGCCA TCTTTGGGAT GTCCAACTTT 
GCCTATGTTA AGAGGGAAGT TGGGATCGAT GACATGTTCA ACTTTGAGAC CTTTGGCAAC 
AGCATGATCT GCCTATTCCA AATTACAACC TCTGCTGGCT GGGATGGATT GCTAGCACCC 
ATTCTCAACA GTAAGCCACC CGACTGTGAC CCTAATAAAG TTAACCCTGG AAGCTCAGTT 
AAGGGAGACT GTGGGAACCC ATCTGTTGGA ATTTTCTTTT TTGTCAGTTA CATCATCATA 
TCCTTCCTGG TTGTGGTGAA CATGTACATC GCGGTCATCC TGGAGAACTT CAGTGTTGCT 
ACTGAAGAAA GTGCAGAGCC TCTGAGTGAG GATGACTTTG AGATGTTCTA TGAGGTTTGG 
GAGAAGTTTG ATCCCGATGC AACTCAGTTC ATGGAATTTG AAAAATTATC TCAGTTTGCA 
GCTGCGCTTG AACCGCCTCT CAATCTGCCA CAACCAAACA AACTCCAGCT CATTGCCATG 
GATTTGCCCA TGGTGAGTGG TGACCGGATC CACTGTCTTG ATATCTTATT TGCTTTTACA 
AAGCGGGTTC TAGGAGAGAG TGGAGAGATG GATGCTCTAC GAATACAGAT GGAAGAGCGA 
TTCATGGCTT CCAATCCTTC CAAGGTCTCC TATCAGCCAA TCACTACTAC TTTAAAACGA 
AAACAAGAGG AAGTATCTGC TGTCATTATT CAGCGTGCTT ACAGACGCCA CCTTTTAAAG 
CGAACTGTAA AACAAGCTTC CTTTACGTAC AATAAAAACA AAATCAAAGG TGGGGCTAAT 
CTTCTTATAA AAGAAGACAT GATAATTGAC AGAATAAATG AAAACTCTAT TACAGAAAAA 
ACTGATCTGA CCATGTCCAC TGCAGCTTGT CCACCTTCCT ATGACCGGGT GACAAAGCCA 
ATTGTGGAAA AACATGAGCA AGAAGGCAAA GATGAAAAAG CCAAAGGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_017004651.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860140.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X12, mRNA.

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CloneID	OHu23006
Clone ID Related Accession (Same CDS sequence)	NM_001353957.1 , XM_017004651.1 , NM_001165964.3 , NM_001165964.1 , XM_011511606.2 , XM_017004652.1 , NM_001353958.2 , NM_001353958.1 , NM_001165964.2 , NM_001353957.2
Accession Version	XM_017004651.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860140.1
CDS	250..6195
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017004651.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004651.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_011511606.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509908.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X11, mRNA.

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CloneID	OHu23006
Clone ID Related Accession (Same CDS sequence)	NM_001353957.1 , XM_017004651.1 , NM_001165964.3 , NM_001165964.1 , XM_011511606.2 , XM_017004652.1 , NM_001353958.2 , NM_001353958.1 , NM_001165964.2 , NM_001353957.2
Accession Version	XM_011511606.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511606.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509908.1
CDS	387..6332
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_011511606.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511606.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_017004652.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860141.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X13, mRNA.

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CloneID	OHu23006
Clone ID Related Accession (Same CDS sequence)	NM_001353957.1 , XM_017004651.1 , NM_001165964.3 , NM_001165964.1 , XM_011511606.2 , XM_017004652.1 , NM_001353958.2 , NM_001353958.1 , NM_001165964.2 , NM_001353957.2
Accession Version	XM_017004652.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5946bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860141.1
CDS	290..6235
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017004652.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004652.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG GAACAACCAC TGAAACTGAA 
ATGAGAAAGA GAAGGTCAAG TTCTTTCCAC GTTTCCATGG ACTTTCTAGA AGATCCTTCC 
CAAAGGCAAC GAGCAATGAG TATAGCCAGC ATTCTAACAA ATACAGTAGA AGAACTTGAA 
GAATCCAGGC AGAAATGCCC ACCCTGTTGG TATAAATTTT CCAACATATT CTTAATCTGG 
GACTGTTCTC CATATTGGTT AAAAGTGAAA CATGTTGTCA ACCTGGTTGT GATGGACCCA 
TTTGTTGACC TGGCCATCAC CATCTGTATT GTCTTAAATA CTCTTTTCAT GGCCATGGAG 
CACTATCCAA TGACGGACCA TTTCAATAAT GTGCTTACAG TAGGAAACTT GGTTTTCACT 
GGGATCTTTA CAGCAGAAAT GTTTCTGAAA ATTATTGCCA TGGATCCTTA CTATTATTTC 
CAAGAAGGCT GGAATATCTT TGACGGTTTT ATTGTGACGC TTAGCCTGGT AGAACTTGGA 
CTCGCCAATG TGGAAGGATT ATCTGTTCTC CGTTCATTTC GATTGCTGCG AGTTTTCAAG 
TTGGCAAAAT CTTGGCCAAC GTTAAATATG CTAATAAAGA TCATCGGCAA TTCCGTGGGG 
GCTCTGGGAA ATTTAACCCT CGTCTTGGCC ATCATCGTCT TCATTTTTGC CGTGGTCGGC 
ATGCAGCTCT TTGGTAAAAG CTACAAAGAT TGTGTCTGCA AGATCGCCAG TGATTGTCAA 
CTCCCACGCT GGCACATGAA TGACTTCTTC CACTCCTTCC TGATTGTGTT CCGCGTGCTG 
TGTGGGGAGT GGATAGAGAC CATGTGGGAC TGTATGGAGG TTGCTGGTCA AGCCATGTGC 
CTTACTGTCT TCATGATGGT CATGGTGATT GGAAACCTAG TGGTCCTGAA TCTCTTTCTG 
GCCTTGCTTC TGAGCTCATT TAGTGCAGAC AACCTTGCAG CCACTGATGA TGATAATGAA 
ATGAATAATC TCCAAATTGC TGTGGATAGG ATGCACAAAG GAGTAGCTTA TGTGAAAAGA 
AAAATATATG AATTTATTCA ACAGTCCTTC ATTAGGAAAC AAAAGATTTT AGATGAAATT 
AAACCACTTG ATGATCTAAA CAACAAGAAA GACAGTTGTA TGTCCAATCA TACAGCAGAA 
ATTGGGAAAG ATCTTGACTA TCTTAAAGAT GTAAATGGAA CTACAAGTGG TATAGGAACT 
GGCAGCAGTG TTGAAAAATA CATTATTGAT GAAAGTGATT ACATGTCATT CATAAACAAC 
CCCAGTCTTA CTGTGACTGT ACCAATTGCT GTAGGAGAAT CTGACTTTGA AAATTTAAAC 
ACGGAAGACT TTAGTAGTGA ATCGGATCTG GAAGAAAGCA AAGAGAAACT GAATGAAAGC 
AGTAGCTCAT CAGAAGGTAG CACTGTGGAC ATCGGCGCAC CTGTAGAAGA ACAGCCCGTA 
GTGGAACCTG AAGAAACTCT TGAACCAGAA GCTTGTTTCA CTGAAGGCTG TGTACAAAGA 
TTCAAGTGTT GTCAAATCAA TGTGGAAGAA GGCAGAGGAA AACAATGGTG GAACCTGAGA 
AGGACGTGTT TCCGAATAGT TGAACATAAC TGGTTTGAGA CCTTCATTGT TTTCATGATT 
CTCCTTAGTA GTGGTGCTCT GGCATTTGAA GATATATATA TTGATCAGCG AAAGACGATT 
AAGACGATGT TGGAATATGC TGACAAGGTT TTCACTTACA TTTTCATTCT GGAAATGCTT 
CTAAAATGGG TGGCATATGG CTATCAAACA TATTTCACCA ATGCCTGGTG TTGGCTGGAC 
TTCTTAATTG TTGATGTTTC ATTGGTCAGT TTAACAGCAA ATGCCTTGGG TTACTCAGAA 
CTTGGAGCCA TCAAATCTCT CAGGACACTA AGAGCTCTGA GACCTCTAAG AGCCTTATCT 
CGATTTGAAG GGATGAGGGT GGTTGTGAAT GCCCTTTTAG GAGCAATTCC ATCCATCATG 
AATGTGCTTC TGGTTTGTCT TATATTCTGG CTAATTTTCA GCATCATGGG CGTAAATTTG 
TTTGCTGGCA AATTCTACCA CTGTATTAAC ACCACAACTG GTGACAGGTT TGACATCGAA 
GACGTGAATA ATCATACTGA TTGCCTAAAA CTAATAGAAA GAAATGAGAC TGCTCGATGG 
AAAAATGTGA AAGTAAACTT TGATAATGTA GGATTTGGGT ATCTCTCTTT GCTTCAAGTT 
GCCACATTCA AAGGATGGAT GGATATAATG TATGCAGCAG TTGATTCCAG AAATGTGGAA 
CTCCAGCCTA AGTATGAAGA AAGTCTGTAC ATGTATCTTT ACTTTGTTAT TTTCATCATC 
TTTGGGTCCT TCTTCACCTT GAACCTGTTT ATTGGTGTCA TCATAGATAA TTTCAACCAG 
CAGAAAAAGA AGTTTGGAGG TCAAGACATC TTTATGACAG AAGAACAGAA GAAATACTAT 
AATGCAATGA AAAAATTAGG ATCGAAAAAA CCGCAAAAGC CTATACCTCG ACCAGGAAAC 
AAATTTCAAG GAATGGTCTT TGACTTCGTA ACCAGACAAG TTTTTGACAT AAGCATCATG 
ATTCTCATCT GTCTTAACAT GGTCACAATG ATGGTGGAAA CAGATGACCA GAGTGAATAT 
GTGACTACCA TTTTGTCACG CATCAATCTG GTGTTCATTG TGCTATTTAC TGGAGAGTGT 
GTACTGAAAC TCATCTCTCT ACGCCATTAT TATTTTACCA TTGGATGGAA TATTTTTGAT 
TTTGTGGTTG TCATTCTCTC CATTGTAGGT ATGTTTCTTG CCGAGCTGAT AGAAAAGTAT 
TTCGTGTCCC CTACCCTGTT CCGAGTGATC CGTCTTGCTA GGATTGGCCG AATCCTACGT 
CTGATCAAAG GAGCAAAGGG GATCCGCACG CTGCTCTTTG CTTTGATGAT GTCCCTTCCT 
GCGTTGTTTA ACATCGGCCT CCTACTCTTC CTAGTCATGT TCATCTACGC CATCTTTGGG 
ATGTCCAACT TTGCCTATGT TAAGAGGGAA GTTGGGATCG ATGACATGTT CAACTTTGAG 
ACCTTTGGCA ACAGCATGAT CTGCCTATTC CAAATTACAA CCTCTGCTGG CTGGGATGGA 
TTGCTAGCAC CCATTCTCAA CAGTAAGCCA CCCGACTGTG ACCCTAATAA AGTTAACCCT 
GGAAGCTCAG TTAAGGGAGA CTGTGGGAAC CCATCTGTTG GAATTTTCTT TTTTGTCAGT 
TACATCATCA TATCCTTCCT GGTTGTGGTG AACATGTACA TCGCGGTCAT CCTGGAGAAC 
TTCAGTGTTG CTACTGAAGA AAGTGCAGAG CCTCTGAGTG AGGATGACTT TGAGATGTTC 
TATGAGGTTT GGGAGAAGTT TGATCCCGAT GCAACTCAGT TCATGGAATT TGAAAAATTA 
TCTCAGTTTG CAGCTGCGCT TGAACCGCCT CTCAATCTGC CACAACCAAA CAAACTCCAG 
CTCATTGCCA TGGATTTGCC CATGGTGAGT GGTGACCGGA TCCACTGTCT TGATATCTTA 
TTTGCTTTTA CAAAGCGGGT TCTAGGAGAG AGTGGAGAGA TGGATGCTCT ACGAATACAG 
ATGGAAGAGC GATTCATGGC TTCCAATCCT TCCAAGGTCT CCTATCAGCC AATCACTACT 
ACTTTAAAAC GAAAACAAGA GGAAGTATCT GCTGTCATTA TTCAGCGTGC TTACAGACGC 
CACCTTTTAA AGCGAACTGT AAAACAAGCT TCCTTTACGT ACAATAAAAA CAAAATCAAA 
GGTGGGGCTA ATCTTCTTAT AAAAGAAGAC ATGATAATTG ACAGAATAAA TGAAAACTCT 
ATTACAGAAA AAACTGATCT GACCATGTCC ACTGCAGCTT GTCCACCTTC CTATGACCGG 
GTGACAAAGC CAATTGTGGA AAAACATGAG CAAGAAGGCA AAGATGAAAA AGCCAAAGGG 
AAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_017004645.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860134.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X3, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	XM_017004645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860134.1
CDS	395..6391
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017004645.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004645.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_017004646.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860135.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X4, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	XM_017004646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860135.1
CDS	309..6305
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017004646.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004646.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_017004648.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860137.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X7, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	XM_017004648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860137.1
CDS	258..6254
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017004648.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004648.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_011511604.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509906.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X5, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	XM_011511604.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511604.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509906.1
CDS	188..6184
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_011511604.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511604.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_017004647.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860136.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X6, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	XM_017004647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860136.1
CDS	481..6477
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017004647.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004647.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_017004649.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860138.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X8, mRNA.

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CloneID	OHu25755
Clone ID Related Accession (Same CDS sequence)	XM_017004645.1 , XM_017004646.1 , NM_001353949.1 , NM_006920.6 , NM_006920.4 , NM_001353952.1 , XM_017004648.1 , NM_001353949.2 , NM_001353952.2 , NM_001353951.1 , NM_001353950.1 , NM_001353950.2 , NM_001353951.2 , NM_006920.5 , XM_011511604.2 , XM_017004647.1 , XM_017004649.1
Accession Version	XM_017004649.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5997bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860138.1
CDS	220..6216
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017004649.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004649.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGCAG 
GCAGCAACGG CAACTGCCTC AGAACATTCC AGAGAGCCCA GTGCAGCAGG CAGGCTCTCA 
GACAGCTCAT CTGAAGCCTC TAAGTTGAGT TCCAAGAGTG CTAAGGAAAG AAGAAATCGG 
AGGAAGAAAA GAAAACAGAA AGAGCAGTCT GGTGGGGAAG AGAAAGATGA GGATGAATTC 
CAAAAATCTG AATCTGAGGA CAGCATCAGG AGGAAAGGTT TTCGCTTCTC CATTGAAGGG 
AACCGATTGA CATATGAAAA GAGGTACTCC TCCCCACACC AGTCTTTGTT GAGCATCCGT 
GGCTCCCTAT TTTCACCAAG GCGAAATAGC AGAACAAGCC TTTTCAGCTT TAGAGGGCGA 
GCAAAGGATG TGGGATCTGA GAACGACTTC GCAGATGATG AGCACAGCAC CTTTGAGGAT 
AACGAGAGCC GTAGAGATTC CTTGTTTGTG CCCCGACGAC ACGGAGAGAG ACGCAACAGC 
AACCTGAGTC AGACCAGTAG GTCATCCCGG ATGCTGGCAG TGTTTCCAGC GAATGGGAAG 
ATGCACAGCA CTGTGGATTG CAATGGTGTG GTTTCCTTGG TTGGTGGACC TTCAGTTCCT 
ACATCGCCTG TTGGACAGCT TCTGCCAGAG GGAACAACCA CTGAAACTGA AATGAGAAAG 
AGAAGGTCAA GTTCTTTCCA CGTTTCCATG GACTTTCTAG AAGATCCTTC CCAAAGGCAA 
CGAGCAATGA GTATAGCCAG CATTCTAACA AATACAGTAG AAGAACTTGA AGAATCCAGG 
CAGAAATGCC CACCCTGTTG GTATAAATTT TCCAACATAT TCTTAATCTG GGACTGTTCT 
CCATATTGGT TAAAAGTGAA ACATGTTGTC AACCTGGTTG TGATGGACCC ATTTGTTGAC 
CTGGCCATCA CCATCTGTAT TGTCTTAAAT ACTCTTTTCA TGGCCATGGA GCACTATCCA 
ATGACGGACC ATTTCAATAA TGTGCTTACA GTAGGAAACT TGGTTTTCAC TGGGATCTTT 
ACAGCAGAAA TGTTTCTGAA AATTATTGCC ATGGATCCTT ACTATTATTT CCAAGAAGGC 
TGGAATATCT TTGACGGTTT TATTGTGACG CTTAGCCTGG TAGAACTTGG ACTCGCCAAT 
GTGGAAGGAT TATCTGTTCT CCGTTCATTT CGATTGCTGC GAGTTTTCAA GTTGGCAAAA 
TCTTGGCCAA CGTTAAATAT GCTAATAAAG ATCATCGGCA ATTCCGTGGG GGCTCTGGGA 
AATTTAACCC TCGTCTTGGC CATCATCGTC TTCATTTTTG CCGTGGTCGG CATGCAGCTC 
TTTGGTAAAA GCTACAAAGA TTGTGTCTGC AAGATCGCCA GTGATTGTCA ACTCCCACGC 
TGGCACATGA ATGACTTCTT CCACTCCTTC CTGATTGTGT TCCGCGTGCT GTGTGGGGAG 
TGGATAGAGA CCATGTGGGA CTGTATGGAG GTTGCTGGTC AAGCCATGTG CCTTACTGTC 
TTCATGATGG TCATGGTGAT TGGAAACCTA GTGGTCCTGA ATCTCTTTCT GGCCTTGCTT 
CTGAGCTCAT TTAGTGCAGA CAACCTTGCA GCCACTGATG ATGATAATGA AATGAATAAT 
CTCCAAATTG CTGTGGATAG GATGCACAAA GGAGTAGCTT ATGTGAAAAG AAAAATATAT 
GAATTTATTC AACAGTCCTT CATTAGGAAA CAAAAGATTT TAGATGAAAT TAAACCACTT 
GATGATCTAA ACAACAAGAA AGACAGTTGT ATGTCCAATC ATACAGCAGA AATTGGGAAA 
GATCTTGACT ATCTTAAAGA TGTAAATGGA ACTACAAGTG GTATAGGAAC TGGCAGCAGT 
GTTGAAAAAT ACATTATTGA TGAAAGTGAT TACATGTCAT TCATAAACAA CCCCAGTCTT 
ACTGTGACTG TACCAATTGC TGTAGGAGAA TCTGACTTTG AAAATTTAAA CACGGAAGAC 
TTTAGTAGTG AATCGGATCT GGAAGAAAGC AAAGAGAAAC TGAATGAAAG CAGTAGCTCA 
TCAGAAGGTA GCACTGTGGA CATCGGCGCA CCTGTAGAAG AACAGCCCGT AGTGGAACCT 
GAAGAAACTC TTGAACCAGA AGCTTGTTTC ACTGAAGGCT GTGTACAAAG ATTCAAGTGT 
TGTCAAATCA ATGTGGAAGA AGGCAGAGGA AAACAATGGT GGAACCTGAG AAGGACGTGT 
TTCCGAATAG TTGAACATAA CTGGTTTGAG ACCTTCATTG TTTTCATGAT TCTCCTTAGT 
AGTGGTGCTC TGGCATTTGA AGATATATAT ATTGATCAGC GAAAGACGAT TAAGACGATG 
TTGGAATATG CTGACAAGGT TTTCACTTAC ATTTTCATTC TGGAAATGCT TCTAAAATGG 
GTGGCATATG GCTATCAAAC ATATTTCACC AATGCCTGGT GTTGGCTGGA CTTCTTAATT 
GTTGATGTTT CATTGGTCAG TTTAACAGCA AATGCCTTGG GTTACTCAGA ACTTGGAGCC 
ATCAAATCTC TCAGGACACT AAGAGCTCTG AGACCTCTAA GAGCCTTATC TCGATTTGAA 
GGGATGAGGG TGGTTGTGAA TGCCCTTTTA GGAGCAATTC CATCCATCAT GAATGTGCTT 
CTGGTTTGTC TTATATTCTG GCTAATTTTC AGCATCATGG GCGTAAATTT GTTTGCTGGC 
AAATTCTACC ACTGTATTAA CACCACAACT GGTGACAGGT TTGACATCGA AGACGTGAAT 
AATCATACTG ATTGCCTAAA ACTAATAGAA AGAAATGAGA CTGCTCGATG GAAAAATGTG 
AAAGTAAACT TTGATAATGT AGGATTTGGG TATCTCTCTT TGCTTCAAGT TGCCACATTC 
AAAGGATGGA TGGATATAAT GTATGCAGCA GTTGATTCCA GAAATGTGGA ACTCCAGCCT 
AAGTATGAAG AAAGTCTGTA CATGTATCTT TACTTTGTTA TTTTCATCAT CTTTGGGTCC 
TTCTTCACCT TGAACCTGTT TATTGGTGTC ATCATAGATA ATTTCAACCA GCAGAAAAAG 
AAGTTTGGAG GTCAAGACAT CTTTATGACA GAAGAACAGA AGAAATACTA TAATGCAATG 
AAAAAATTAG GATCGAAAAA ACCGCAAAAG CCTATACCTC GACCAGGAAA CAAATTTCAA 
GGAATGGTCT TTGACTTCGT AACCAGACAA GTTTTTGACA TAAGCATCAT GATTCTCATC 
TGTCTTAACA TGGTCACAAT GATGGTGGAA ACAGATGACC AGAGTGAATA TGTGACTACC 
ATTTTGTCAC GCATCAATCT GGTGTTCATT GTGCTATTTA CTGGAGAGTG TGTACTGAAA 
CTCATCTCTC TACGCCATTA TTATTTTACC ATTGGATGGA ATATTTTTGA TTTTGTGGTT 
GTCATTCTCT CCATTGTAGG TATGTTTCTT GCCGAGCTGA TAGAAAAGTA TTTCGTGTCC 
CCTACCCTGT TCCGAGTGAT CCGTCTTGCT AGGATTGGCC GAATCCTACG TCTGATCAAA 
GGAGCAAAGG GGATCCGCAC GCTGCTCTTT GCTTTGATGA TGTCCCTTCC TGCGTTGTTT 
AACATCGGCC TCCTACTCTT CCTAGTCATG TTCATCTACG CCATCTTTGG GATGTCCAAC 
TTTGCCTATG TTAAGAGGGA AGTTGGGATC GATGACATGT TCAACTTTGA GACCTTTGGC 
AACAGCATGA TCTGCCTATT CCAAATTACA ACCTCTGCTG GCTGGGATGG ATTGCTAGCA 
CCCATTCTCA ACAGTAAGCC ACCCGACTGT GACCCTAATA AAGTTAACCC TGGAAGCTCA 
GTTAAGGGAG ACTGTGGGAA CCCATCTGTT GGAATTTTCT TTTTTGTCAG TTACATCATC 
ATATCCTTCC TGGTTGTGGT GAACATGTAC ATCGCGGTCA TCCTGGAGAA CTTCAGTGTT 
GCTACTGAAG AAAGTGCAGA GCCTCTGAGT GAGGATGACT TTGAGATGTT CTATGAGGTT 
TGGGAGAAGT TTGATCCCGA TGCAACTCAG TTCATGGAAT TTGAAAAATT ATCTCAGTTT 
GCAGCTGCGC TTGAACCGCC TCTCAATCTG CCACAACCAA ACAAACTCCA GCTCATTGCC 
ATGGATTTGC CCATGGTGAG TGGTGACCGG ATCCACTGTC TTGATATCTT ATTTGCTTTT 
ACAAAGCGGG TTCTAGGAGA GAGTGGAGAG ATGGATGCTC TACGAATACA GATGGAAGAG 
CGATTCATGG CTTCCAATCC TTCCAAGGTC TCCTATCAGC CAATCACTAC TACTTTAAAA 
CGAAAACAAG AGGAAGTATC TGCTGTCATT ATTCAGCGTG CTTACAGACG CCACCTTTTA 
AAGCGAACTG TAAAACAAGC TTCCTTTACG TACAATAAAA ACAAAATCAA AGGTGGGGCT 
AATCTTCTTA TAAAAGAAGA CATGATAATT GACAGAATAA ATGAAAACTC TATTACAGAA 
AAAACTGATC TGACCATGTC CACTGCAGCT TGTCCACCTT CCTATGACCG GGTGACAAAG 
CCAATTGTGG AAAAACATGA GCAAGAAGGC AAAGATGAAA AAGCCAAAGG GAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_011511605.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011509907.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X9, mRNA.

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CloneID	OHu60371
Clone ID Related Accession (Same CDS sequence)	XM_011511605.2 , NM_001353954.1 , NM_001353955.1 , NM_001353954.2 , NM_001353955.2 , XM_017004650.1
Accession Version	XM_011511605.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511605.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGTTG GTGGACCTTC AGTTCCTACA 
TCGCCTGTTG GACAGCTTCT GCCAGAGGGA ACAACCACTG AAACTGAAAT GAGAAAGAGA 
AGGTCAAGTT CTTTCCACGT TTCCATGGAC TTTCTAGAAG ATCCTTCCCA AAGGCAACGA 
GCAATGAGTA TAGCCAGCAT TCTAACAAAT ACAGTAGAAG AACTTGAAGA ATCCAGGCAG 
AAATGCCCAC CCTGTTGGTA TAAATTTTCC AACATATTCT TAATCTGGGA CTGTTCTCCA 
TATTGGTTAA AAGTGAAACA TGTTGTCAAC CTGGTTGTGA TGGACCCATT TGTTGACCTG 
GCCATCACCA TCTGTATTGT CTTAAATACT CTTTTCATGG CCATGGAGCA CTATCCAATG 
ACGGACCATT TCAATAATGT GCTTACAGTA GGAAACTTGG TTTTCACTGG GATCTTTACA 
GCAGAAATGT TTCTGAAAAT TATTGCCATG GATCCTTACT ATTATTTCCA AGAAGGCTGG 
AATATCTTTG ACGGTTTTAT TGTGACGCTT AGCCTGGTAG AACTTGGACT CGCCAATGTG 
GAAGGATTAT CTGTTCTCCG TTCATTTCGA TTGCTGCGAG TTTTCAAGTT GGCAAAATCT 
TGGCCAACGT TAAATATGCT AATAAAGATC ATCGGCAATT CCGTGGGGGC TCTGGGAAAT 
TTAACCCTCG TCTTGGCCAT CATCGTCTTC ATTTTTGCCG TGGTCGGCAT GCAGCTCTTT 
GGTAAAAGCT ACAAAGATTG TGTCTGCAAG ATCGCCAGTG ATTGTCAACT CCCACGCTGG 
CACATGAATG ACTTCTTCCA CTCCTTCCTG ATTGTGTTCC GCGTGCTGTG TGGGGAGTGG 
ATAGAGACCA TGTGGGACTG TATGGAGGTT GCTGGTCAAG CCATGTGCCT TACTGTCTTC 
ATGATGGTCA TGGTGATTGG AAACCTAGTG GTCCTGAATC TCTTTCTGGC CTTGCTTCTG 
AGCTCATTTA GTGCAGACAA CCTTGCAGCC ACTGATGATG ATAATGAAAT GAATAATCTC 
CAAATTGCTG TGGATAGGAT GCACAAAGGA GTAGCTTATG TGAAAAGAAA AATATATGAA 
TTTATTCAAC AGTCCTTCAT TAGGAAACAA AAGATTTTAG ATGAAATTAA ACCACTTGAT 
GATCTAAACA ACAAGAAAGA CAGTTGTATG TCCAATCATA CAGCAGAAAT TGGGAAAGAT 
CTTGACTATC TTAAAGATGT AAATGGAACT ACAAGTGGTA TAGGAACTGG CAGCAGTGTT 
GAAAAATACA TTATTGATGA AAGTGATTAC ATGTCATTCA TAAACAACCC CAGTCTTACT 
GTGACTGTAC CAATTGCTGT AGGAGAATCT GACTTTGAAA ATTTAAACAC GGAAGACTTT 
AGTAGTGAAT CGGATCTGGA AGAAAGCAAA GAGAAACTGA ATGAAAGCAG TAGCTCATCA 
GAAGGTAGCA CTGTGGACAT CGGCGCACCT GTAGAAGAAC AGCCCGTAGT GGAACCTGAA 
GAAACTCTTG AACCAGAAGC TTGTTTCACT GAAGGCTGTG TACAAAGATT CAAGTGTTGT 
CAAATCAATG TGGAAGAAGG CAGAGGAAAA CAATGGTGGA ACCTGAGAAG GACGTGTTTC 
CGAATAGTTG AACATAACTG GTTTGAGACC TTCATTGTTT TCATGATTCT CCTTAGTAGT 
GGTGCTCTGG CATTTGAAGA TATATATATT GATCAGCGAA AGACGATTAA GACGATGTTG 
GAATATGCTG ACAAGGTTTT CACTTACATT TTCATTCTGG AAATGCTTCT AAAATGGGTG 
GCATATGGCT ATCAAACATA TTTCACCAAT GCCTGGTGTT GGCTGGACTT CTTAATTGTT 
GATGTTTCAT TGGTCAGTTT AACAGCAAAT GCCTTGGGTT ACTCAGAACT TGGAGCCATC 
AAATCTCTCA GGACACTAAG AGCTCTGAGA CCTCTAAGAG CCTTATCTCG ATTTGAAGGG 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA TGTGCTTCTG 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGCG TAAATTTGTT TGCTGGCAAA 
TTCTACCACT GTATTAACAC CACAACTGGT GACAGGTTTG ACATCGAAGA CGTGAATAAT 
CATACTGATT GCCTAAAACT AATAGAAAGA AATGAGACTG CTCGATGGAA AAATGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTCTCTTTGC TTCAAGTTGC CACATTCAAA 
GGATGGATGG ATATAATGTA TGCAGCAGTT GATTCCAGAA ATGTGGAACT CCAGCCTAAG 
TATGAAGAAA GTCTGTACAT GTATCTTTAC TTTGTTATTT TCATCATCTT TGGGTCCTTC 
TTCACCTTGA ACCTGTTTAT TGGTGTCATC ATAGATAATT TCAACCAGCA GAAAAAGAAG 
TTTGGAGGTC AAGACATCTT TATGACAGAA GAACAGAAGA AATACTATAA TGCAATGAAA 
AAATTAGGAT CGAAAAAACC GCAAAAGCCT ATACCTCGAC CAGGAAACAA ATTTCAAGGA 
ATGGTCTTTG ACTTCGTAAC CAGACAAGTT TTTGACATAA GCATCATGAT TCTCATCTGT 
CTTAACATGG TCACAATGAT GGTGGAAACA GATGACCAGA GTGAATATGT GACTACCATT 
TTGTCACGCA TCAATCTGGT GTTCATTGTG CTATTTACTG GAGAGTGTGT ACTGAAACTC 
ATCTCTCTAC GCCATTATTA TTTTACCATT GGATGGAATA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCCA TTGTAGGTAT GTTTCTTGCC GAGCTGATAG AAAAGTATTT CGTGTCCCCT 
ACCCTGTTCC GAGTGATCCG TCTTGCTAGG ATTGGCCGAA TCCTACGTCT GATCAAAGGA 
GCAAAGGGGA TCCGCACGCT GCTCTTTGCT TTGATGATGT CCCTTCCTGC GTTGTTTAAC 
ATCGGCCTCC TACTCTTCCT AGTCATGTTC ATCTACGCCA TCTTTGGGAT GTCCAACTTT 
GCCTATGTTA AGAGGGAAGT TGGGATCGAT GACATGTTCA ACTTTGAGAC CTTTGGCAAC 
AGCATGATCT GCCTATTCCA AATTACAACC TCTGCTGGCT GGGATGGATT GCTAGCACCC 
ATTCTCAACA GTAAGCCACC CGACTGTGAC CCTAATAAAG TTAACCCTGG AAGCTCAGTT 
AAGGGAGACT GTGGGAACCC ATCTGTTGGA ATTTTCTTTT TTGTCAGTTA CATCATCATA 
TCCTTCCTGG TTGTGGTGAA CATGTACATC GCGGTCATCC TGGAGAACTT CAGTGTTGCT 
ACTGAAGAAA GTGCAGAGCC TCTGAGTGAG GATGACTTTG AGATGTTCTA TGAGGTTTGG 
GAGAAGTTTG ATCCCGATGC AACTCAGTTC ATGGAATTTG AAAAATTATC TCAGTTTGCA 
GCTGCGCTTG AACCGCCTCT CAATCTGCCA CAACCAAACA AACTCCAGCT CATTGCCATG 
GATTTGCCCA TGGTGAGTGG TGACCGGATC CACTGTCTTG ATATCTTATT TGCTTTTACA 
AAGCGGGTTC TAGGAGAGAG TGGAGAGATG GATGCTCTAC GAATACAGAT GGAAGAGCGA 
TTCATGGCTT CCAATCCTTC CAAGGTCTCC TATCAGCCAA TCACTACTAC TTTAAAACGA 
AAACAAGAGG AAGTATCTGC TGTCATTATT CAGCGTGCTT ACAGACGCCA CCTTTTAAAG 
CGAACTGTAA AACAAGCTTC CTTTACGTAC AATAAAAACA AAATCAAAGG TGGGGCTAAT 
CTTCTTATAA AAGAAGACAT GATAATTGAC AGAATAAATG AAAACTCTAT TACAGAAAAA 
ACTGATCTGA CCATGTCCAC TGCAGCTTGT CCACCTTCCT ATGACCGGGT GACAAAGCCA 
ATTGTGGAAA AACATGAGCA AGAAGGCAAA GATGAAAAAG CCAAAGGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011509907.1
CDS	395..6388
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_011511605.2
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011511605.2

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGTTG GTGGACCTTC AGTTCCTACA 
TCGCCTGTTG GACAGCTTCT GCCAGAGGGA ACAACCACTG AAACTGAAAT GAGAAAGAGA 
AGGTCAAGTT CTTTCCACGT TTCCATGGAC TTTCTAGAAG ATCCTTCCCA AAGGCAACGA 
GCAATGAGTA TAGCCAGCAT TCTAACAAAT ACAGTAGAAG AACTTGAAGA ATCCAGGCAG 
AAATGCCCAC CCTGTTGGTA TAAATTTTCC AACATATTCT TAATCTGGGA CTGTTCTCCA 
TATTGGTTAA AAGTGAAACA TGTTGTCAAC CTGGTTGTGA TGGACCCATT TGTTGACCTG 
GCCATCACCA TCTGTATTGT CTTAAATACT CTTTTCATGG CCATGGAGCA CTATCCAATG 
ACGGACCATT TCAATAATGT GCTTACAGTA GGAAACTTGG TTTTCACTGG GATCTTTACA 
GCAGAAATGT TTCTGAAAAT TATTGCCATG GATCCTTACT ATTATTTCCA AGAAGGCTGG 
AATATCTTTG ACGGTTTTAT TGTGACGCTT AGCCTGGTAG AACTTGGACT CGCCAATGTG 
GAAGGATTAT CTGTTCTCCG TTCATTTCGA TTGCTGCGAG TTTTCAAGTT GGCAAAATCT 
TGGCCAACGT TAAATATGCT AATAAAGATC ATCGGCAATT CCGTGGGGGC TCTGGGAAAT 
TTAACCCTCG TCTTGGCCAT CATCGTCTTC ATTTTTGCCG TGGTCGGCAT GCAGCTCTTT 
GGTAAAAGCT ACAAAGATTG TGTCTGCAAG ATCGCCAGTG ATTGTCAACT CCCACGCTGG 
CACATGAATG ACTTCTTCCA CTCCTTCCTG ATTGTGTTCC GCGTGCTGTG TGGGGAGTGG 
ATAGAGACCA TGTGGGACTG TATGGAGGTT GCTGGTCAAG CCATGTGCCT TACTGTCTTC 
ATGATGGTCA TGGTGATTGG AAACCTAGTG GTCCTGAATC TCTTTCTGGC CTTGCTTCTG 
AGCTCATTTA GTGCAGACAA CCTTGCAGCC ACTGATGATG ATAATGAAAT GAATAATCTC 
CAAATTGCTG TGGATAGGAT GCACAAAGGA GTAGCTTATG TGAAAAGAAA AATATATGAA 
TTTATTCAAC AGTCCTTCAT TAGGAAACAA AAGATTTTAG ATGAAATTAA ACCACTTGAT 
GATCTAAACA ACAAGAAAGA CAGTTGTATG TCCAATCATA CAGCAGAAAT TGGGAAAGAT 
CTTGACTATC TTAAAGATGT AAATGGAACT ACAAGTGGTA TAGGAACTGG CAGCAGTGTT 
GAAAAATACA TTATTGATGA AAGTGATTAC ATGTCATTCA TAAACAACCC CAGTCTTACT 
GTGACTGTAC CAATTGCTGT AGGAGAATCT GACTTTGAAA ATTTAAACAC GGAAGACTTT 
AGTAGTGAAT CGGATCTGGA AGAAAGCAAA GAGAAACTGA ATGAAAGCAG TAGCTCATCA 
GAAGGTAGCA CTGTGGACAT CGGCGCACCT GTAGAAGAAC AGCCCGTAGT GGAACCTGAA 
GAAACTCTTG AACCAGAAGC TTGTTTCACT GAAGGCTGTG TACAAAGATT CAAGTGTTGT 
CAAATCAATG TGGAAGAAGG CAGAGGAAAA CAATGGTGGA ACCTGAGAAG GACGTGTTTC 
CGAATAGTTG AACATAACTG GTTTGAGACC TTCATTGTTT TCATGATTCT CCTTAGTAGT 
GGTGCTCTGG CATTTGAAGA TATATATATT GATCAGCGAA AGACGATTAA GACGATGTTG 
GAATATGCTG ACAAGGTTTT CACTTACATT TTCATTCTGG AAATGCTTCT AAAATGGGTG 
GCATATGGCT ATCAAACATA TTTCACCAAT GCCTGGTGTT GGCTGGACTT CTTAATTGTT 
GATGTTTCAT TGGTCAGTTT AACAGCAAAT GCCTTGGGTT ACTCAGAACT TGGAGCCATC 
AAATCTCTCA GGACACTAAG AGCTCTGAGA CCTCTAAGAG CCTTATCTCG ATTTGAAGGG 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA TGTGCTTCTG 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGCG TAAATTTGTT TGCTGGCAAA 
TTCTACCACT GTATTAACAC CACAACTGGT GACAGGTTTG ACATCGAAGA CGTGAATAAT 
CATACTGATT GCCTAAAACT AATAGAAAGA AATGAGACTG CTCGATGGAA AAATGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTCTCTTTGC TTCAAGTTGC CACATTCAAA 
GGATGGATGG ATATAATGTA TGCAGCAGTT GATTCCAGAA ATGTGGAACT CCAGCCTAAG 
TATGAAGAAA GTCTGTACAT GTATCTTTAC TTTGTTATTT TCATCATCTT TGGGTCCTTC 
TTCACCTTGA ACCTGTTTAT TGGTGTCATC ATAGATAATT TCAACCAGCA GAAAAAGAAG 
TTTGGAGGTC AAGACATCTT TATGACAGAA GAACAGAAGA AATACTATAA TGCAATGAAA 
AAATTAGGAT CGAAAAAACC GCAAAAGCCT ATACCTCGAC CAGGAAACAA ATTTCAAGGA 
ATGGTCTTTG ACTTCGTAAC CAGACAAGTT TTTGACATAA GCATCATGAT TCTCATCTGT 
CTTAACATGG TCACAATGAT GGTGGAAACA GATGACCAGA GTGAATATGT GACTACCATT 
TTGTCACGCA TCAATCTGGT GTTCATTGTG CTATTTACTG GAGAGTGTGT ACTGAAACTC 
ATCTCTCTAC GCCATTATTA TTTTACCATT GGATGGAATA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCCA TTGTAGGTAT GTTTCTTGCC GAGCTGATAG AAAAGTATTT CGTGTCCCCT 
ACCCTGTTCC GAGTGATCCG TCTTGCTAGG ATTGGCCGAA TCCTACGTCT GATCAAAGGA 
GCAAAGGGGA TCCGCACGCT GCTCTTTGCT TTGATGATGT CCCTTCCTGC GTTGTTTAAC 
ATCGGCCTCC TACTCTTCCT AGTCATGTTC ATCTACGCCA TCTTTGGGAT GTCCAACTTT 
GCCTATGTTA AGAGGGAAGT TGGGATCGAT GACATGTTCA ACTTTGAGAC CTTTGGCAAC 
AGCATGATCT GCCTATTCCA AATTACAACC TCTGCTGGCT GGGATGGATT GCTAGCACCC 
ATTCTCAACA GTAAGCCACC CGACTGTGAC CCTAATAAAG TTAACCCTGG AAGCTCAGTT 
AAGGGAGACT GTGGGAACCC ATCTGTTGGA ATTTTCTTTT TTGTCAGTTA CATCATCATA 
TCCTTCCTGG TTGTGGTGAA CATGTACATC GCGGTCATCC TGGAGAACTT CAGTGTTGCT 
ACTGAAGAAA GTGCAGAGCC TCTGAGTGAG GATGACTTTG AGATGTTCTA TGAGGTTTGG 
GAGAAGTTTG ATCCCGATGC AACTCAGTTC ATGGAATTTG AAAAATTATC TCAGTTTGCA 
GCTGCGCTTG AACCGCCTCT CAATCTGCCA CAACCAAACA AACTCCAGCT CATTGCCATG 
GATTTGCCCA TGGTGAGTGG TGACCGGATC CACTGTCTTG ATATCTTATT TGCTTTTACA 
AAGCGGGTTC TAGGAGAGAG TGGAGAGATG GATGCTCTAC GAATACAGAT GGAAGAGCGA 
TTCATGGCTT CCAATCCTTC CAAGGTCTCC TATCAGCCAA TCACTACTAC TTTAAAACGA 
AAACAAGAGG AAGTATCTGC TGTCATTATT CAGCGTGCTT ACAGACGCCA CCTTTTAAAG 
CGAACTGTAA AACAAGCTTC CTTTACGTAC AATAAAAACA AAATCAAAGG TGGGGCTAAT 
CTTCTTATAA AAGAAGACAT GATAATTGAC AGAATAAATG AAAACTCTAT TACAGAAAAA 
ACTGATCTGA CCATGTCCAC TGCAGCTTGT CCACCTTCCT ATGACCGGGT GACAAAGCCA 
ATTGTGGAAA AACATGAGCA AGAAGGCAAA GATGAAAAAG CCAAAGGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SCN1A ( XM_017004650.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860139.1 Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X10, mRNA.

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CloneID	OHu60371
Clone ID Related Accession (Same CDS sequence)	XM_011511605.2 , NM_001353954.1 , NM_001353955.1 , NM_001353954.2 , NM_001353955.2 , XM_017004650.1
Accession Version	XM_017004650.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	sodium channel protein type 1 subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGTTG GTGGACCTTC AGTTCCTACA 
TCGCCTGTTG GACAGCTTCT GCCAGAGGGA ACAACCACTG AAACTGAAAT GAGAAAGAGA 
AGGTCAAGTT CTTTCCACGT TTCCATGGAC TTTCTAGAAG ATCCTTCCCA AAGGCAACGA 
GCAATGAGTA TAGCCAGCAT TCTAACAAAT ACAGTAGAAG AACTTGAAGA ATCCAGGCAG 
AAATGCCCAC CCTGTTGGTA TAAATTTTCC AACATATTCT TAATCTGGGA CTGTTCTCCA 
TATTGGTTAA AAGTGAAACA TGTTGTCAAC CTGGTTGTGA TGGACCCATT TGTTGACCTG 
GCCATCACCA TCTGTATTGT CTTAAATACT CTTTTCATGG CCATGGAGCA CTATCCAATG 
ACGGACCATT TCAATAATGT GCTTACAGTA GGAAACTTGG TTTTCACTGG GATCTTTACA 
GCAGAAATGT TTCTGAAAAT TATTGCCATG GATCCTTACT ATTATTTCCA AGAAGGCTGG 
AATATCTTTG ACGGTTTTAT TGTGACGCTT AGCCTGGTAG AACTTGGACT CGCCAATGTG 
GAAGGATTAT CTGTTCTCCG TTCATTTCGA TTGCTGCGAG TTTTCAAGTT GGCAAAATCT 
TGGCCAACGT TAAATATGCT AATAAAGATC ATCGGCAATT CCGTGGGGGC TCTGGGAAAT 
TTAACCCTCG TCTTGGCCAT CATCGTCTTC ATTTTTGCCG TGGTCGGCAT GCAGCTCTTT 
GGTAAAAGCT ACAAAGATTG TGTCTGCAAG ATCGCCAGTG ATTGTCAACT CCCACGCTGG 
CACATGAATG ACTTCTTCCA CTCCTTCCTG ATTGTGTTCC GCGTGCTGTG TGGGGAGTGG 
ATAGAGACCA TGTGGGACTG TATGGAGGTT GCTGGTCAAG CCATGTGCCT TACTGTCTTC 
ATGATGGTCA TGGTGATTGG AAACCTAGTG GTCCTGAATC TCTTTCTGGC CTTGCTTCTG 
AGCTCATTTA GTGCAGACAA CCTTGCAGCC ACTGATGATG ATAATGAAAT GAATAATCTC 
CAAATTGCTG TGGATAGGAT GCACAAAGGA GTAGCTTATG TGAAAAGAAA AATATATGAA 
TTTATTCAAC AGTCCTTCAT TAGGAAACAA AAGATTTTAG ATGAAATTAA ACCACTTGAT 
GATCTAAACA ACAAGAAAGA CAGTTGTATG TCCAATCATA CAGCAGAAAT TGGGAAAGAT 
CTTGACTATC TTAAAGATGT AAATGGAACT ACAAGTGGTA TAGGAACTGG CAGCAGTGTT 
GAAAAATACA TTATTGATGA AAGTGATTAC ATGTCATTCA TAAACAACCC CAGTCTTACT 
GTGACTGTAC CAATTGCTGT AGGAGAATCT GACTTTGAAA ATTTAAACAC GGAAGACTTT 
AGTAGTGAAT CGGATCTGGA AGAAAGCAAA GAGAAACTGA ATGAAAGCAG TAGCTCATCA 
GAAGGTAGCA CTGTGGACAT CGGCGCACCT GTAGAAGAAC AGCCCGTAGT GGAACCTGAA 
GAAACTCTTG AACCAGAAGC TTGTTTCACT GAAGGCTGTG TACAAAGATT CAAGTGTTGT 
CAAATCAATG TGGAAGAAGG CAGAGGAAAA CAATGGTGGA ACCTGAGAAG GACGTGTTTC 
CGAATAGTTG AACATAACTG GTTTGAGACC TTCATTGTTT TCATGATTCT CCTTAGTAGT 
GGTGCTCTGG CATTTGAAGA TATATATATT GATCAGCGAA AGACGATTAA GACGATGTTG 
GAATATGCTG ACAAGGTTTT CACTTACATT TTCATTCTGG AAATGCTTCT AAAATGGGTG 
GCATATGGCT ATCAAACATA TTTCACCAAT GCCTGGTGTT GGCTGGACTT CTTAATTGTT 
GATGTTTCAT TGGTCAGTTT AACAGCAAAT GCCTTGGGTT ACTCAGAACT TGGAGCCATC 
AAATCTCTCA GGACACTAAG AGCTCTGAGA CCTCTAAGAG CCTTATCTCG ATTTGAAGGG 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA TGTGCTTCTG 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGCG TAAATTTGTT TGCTGGCAAA 
TTCTACCACT GTATTAACAC CACAACTGGT GACAGGTTTG ACATCGAAGA CGTGAATAAT 
CATACTGATT GCCTAAAACT AATAGAAAGA AATGAGACTG CTCGATGGAA AAATGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTCTCTTTGC TTCAAGTTGC CACATTCAAA 
GGATGGATGG ATATAATGTA TGCAGCAGTT GATTCCAGAA ATGTGGAACT CCAGCCTAAG 
TATGAAGAAA GTCTGTACAT GTATCTTTAC TTTGTTATTT TCATCATCTT TGGGTCCTTC 
TTCACCTTGA ACCTGTTTAT TGGTGTCATC ATAGATAATT TCAACCAGCA GAAAAAGAAG 
TTTGGAGGTC AAGACATCTT TATGACAGAA GAACAGAAGA AATACTATAA TGCAATGAAA 
AAATTAGGAT CGAAAAAACC GCAAAAGCCT ATACCTCGAC CAGGAAACAA ATTTCAAGGA 
ATGGTCTTTG ACTTCGTAAC CAGACAAGTT TTTGACATAA GCATCATGAT TCTCATCTGT 
CTTAACATGG TCACAATGAT GGTGGAAACA GATGACCAGA GTGAATATGT GACTACCATT 
TTGTCACGCA TCAATCTGGT GTTCATTGTG CTATTTACTG GAGAGTGTGT ACTGAAACTC 
ATCTCTCTAC GCCATTATTA TTTTACCATT GGATGGAATA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCCA TTGTAGGTAT GTTTCTTGCC GAGCTGATAG AAAAGTATTT CGTGTCCCCT 
ACCCTGTTCC GAGTGATCCG TCTTGCTAGG ATTGGCCGAA TCCTACGTCT GATCAAAGGA 
GCAAAGGGGA TCCGCACGCT GCTCTTTGCT TTGATGATGT CCCTTCCTGC GTTGTTTAAC 
ATCGGCCTCC TACTCTTCCT AGTCATGTTC ATCTACGCCA TCTTTGGGAT GTCCAACTTT 
GCCTATGTTA AGAGGGAAGT TGGGATCGAT GACATGTTCA ACTTTGAGAC CTTTGGCAAC 
AGCATGATCT GCCTATTCCA AATTACAACC TCTGCTGGCT GGGATGGATT GCTAGCACCC 
ATTCTCAACA GTAAGCCACC CGACTGTGAC CCTAATAAAG TTAACCCTGG AAGCTCAGTT 
AAGGGAGACT GTGGGAACCC ATCTGTTGGA ATTTTCTTTT TTGTCAGTTA CATCATCATA 
TCCTTCCTGG TTGTGGTGAA CATGTACATC GCGGTCATCC TGGAGAACTT CAGTGTTGCT 
ACTGAAGAAA GTGCAGAGCC TCTGAGTGAG GATGACTTTG AGATGTTCTA TGAGGTTTGG 
GAGAAGTTTG ATCCCGATGC AACTCAGTTC ATGGAATTTG AAAAATTATC TCAGTTTGCA 
GCTGCGCTTG AACCGCCTCT CAATCTGCCA CAACCAAACA AACTCCAGCT CATTGCCATG 
GATTTGCCCA TGGTGAGTGG TGACCGGATC CACTGTCTTG ATATCTTATT TGCTTTTACA 
AAGCGGGTTC TAGGAGAGAG TGGAGAGATG GATGCTCTAC GAATACAGAT GGAAGAGCGA 
TTCATGGCTT CCAATCCTTC CAAGGTCTCC TATCAGCCAA TCACTACTAC TTTAAAACGA 
AAACAAGAGG AAGTATCTGC TGTCATTATT CAGCGTGCTT ACAGACGCCA CCTTTTAAAG 
CGAACTGTAA AACAAGCTTC CTTTACGTAC AATAAAAACA AAATCAAAGG TGGGGCTAAT 
CTTCTTATAA AAGAAGACAT GATAATTGAC AGAATAAATG AAAACTCTAT TACAGAAAAA 
ACTGATCTGA CCATGTCCAC TGCAGCTTGT CCACCTTCCT ATGACCGGGT GACAAAGCCA 
ATTGTGGAAA AACATGAGCA AGAAGGCAAA GATGAAAAAG CCAAAGGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860139.1
CDS	297..6290
Translation	MEQTVLVPPGPDSFNFFTRESLAAIERRIAEEKAKNPKPDKKDDDENGPKPNSDLEAGKNLPFIYGDIPPEMVSEPLEDLDPYYINKKTFIVLNKGKAIFRFSATSALYILTPFNPLRKIAIKILVHSLFSMLIMCTILTNCVFMTMSNPPDWTKNVEYTFTGIYTFESLIKIIARGFCLEDFTFLRDPWNWLDFTVITFAYVTEFVDLGNVSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLRNKCIQWPPTNASLEEHSIEKNITVNYNGTLINETVFEFDWKSYIQDSRYHYFLEGFLDALLCGNSSDAGQCPEGYMCVKAGRNPNYGYTSFDTFSWAFLSLFRLMTQDFWENLYQLTLRAAGKTYMIFFVLVIFLGSFYLINLILAVVAMAYEEQNQATLEEAEQKEAEFQQMIEQLKKQQEAAQAATATASEHSREPSAAGRLSDSSSEASKLSSKSAKERRNRRKKRKQKEQSGGEEKDEDEFQKSESEDSIRRKGFRFSIEGNRLTYEKRYSSPHQSLLSIRGSLFSPRRNSRTSLFSFRGRAKDVGSENDFADDEHSTFEDNESRRDSLFVPRRHGERRNSNLSQTSRSSRMLAVFPANGKMHSTVDCNGVVSLVGGPSVPTSPVGQLLPEGTTTETEMRKRRSSSFHVSMDFLEDPSQRQRAMSIASILTNTVEELEESRQKCPPCWYKFSNIFLIWDCSPYWLKVKHVVNLVVMDPFVDLAITICIVLNTLFMAMEHYPMTDHFNNVLTVGNLVFTGIFTAEMFLKIIAMDPYYYFQEGWNIFDGFIVTLSLVELGLANVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTLVLAIIVFIFAVVGMQLFGKSYKDCVCKIASDCQLPRWHMNDFFHSFLIVFRVLCGEWIETMWDCMEVAGQAMCLTVFMMVMVIGNLVVLNLFLALLLSSFSADNLAATDDDNEMNNLQIAVDRMHKGVAYVKRKIYEFIQQSFIRKQKILDEIKPLDDLNNKKDSCMSNHTAEIGKDLDYLKDVNGTTSGIGTGSSVEKYIIDESDYMSFINNPSLTVTVPIAVGESDFENLNTEDFSSESDLEESKEKLNESSSSSEGSTVDIGAPVEEQPVVEPEETLEPEACFTEGCVQRFKCCQINVEEGRGKQWWNLRRTCFRIVEHNWFETFIVFMILLSSGALAFEDIYIDQRKTIKTMLEYADKVFTYIFILEMLLKWVAYGYQTYFTNAWCWLDFLIVDVSLVSLTANALGYSELGAIKSLRTLRALRPLRALSRFEGMRVVVNALLGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFYHCINTTTGDRFDIEDVNNHTDCLKLIERNETARWKNVKVNFDNVGFGYLSLLQVATFKGWMDIMYAAVDSRNVELQPKYEESLYMYLYFVIFIIFGSFFTLNLFIGVIIDNFNQQKKKFGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPGNKFQGMVFDFVTRQVFDISIMILICLNMVTMMVETDDQSEYVTTILSRINLVFIVLFTGECVLKLISLRHYYFTIGWNIFDFVVVILSIVGMFLAELIEKYFVSPTLFRVIRLARIGRILRLIKGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYAIFGMSNFAYVKREVGIDDMFNFETFGNSMICLFQITTSAGWDGLLAPILNSKPPDCDPNKVNPGSSVKGDCGNPSVGIFFFVSYIIISFLVVVNMYIAVILENFSVATEESAEPLSEDDFEMFYEVWEKFDPDATQFMEFEKLSQFAAALEPPLNLPQPNKLQLIAMDLPMVSGDRIHCLDILFAFTKRVLGESGEMDALRIQMEERFMASNPSKVSYQPITTTLKRKQEEVSAVIIQRAYRRHLLKRTVKQASFTYNKNKIKGGANLLIKEDMIIDRINENSITEKTDLTMSTAACPPSYDRVTKPIVEKHEQEGKDEKAKGK

Target ORF information:

RefSeq Version	XM_017004650.1
Organism	Homo sapiens(human)
Definition	Homo sapiens sodium voltage-gated channel alpha subunit 1 (SCN1A), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004650.1

ORF Insert Sequence:

ATGGAGCAAA CAGTGCTTGT ACCACCAGGA CCTGACAGCT TCAACTTCTT CACCAGAGAA 
TCTCTTGCGG CTATTGAAAG ACGCATTGCA GAAGAAAAGG CAAAGAATCC CAAACCAGAC 
AAAAAAGATG ACGACGAAAA TGGCCCAAAG CCAAATAGTG ACTTGGAAGC TGGAAAGAAC 
CTTCCATTTA TTTATGGAGA CATTCCTCCA GAGATGGTGT CAGAGCCCCT GGAGGACCTG 
GACCCCTACT ATATCAATAA GAAAACTTTT ATAGTATTGA ATAAAGGGAA GGCCATCTTC 
CGGTTCAGTG CCACCTCTGC CCTGTACATT TTAACTCCCT TCAATCCTCT TAGGAAAATA 
GCTATTAAGA TTTTGGTACA TTCATTATTC AGCATGCTAA TTATGTGCAC TATTTTGACA 
AACTGTGTGT TTATGACAAT GAGTAACCCT CCTGATTGGA CAAAGAATGT AGAATACACC 
TTCACAGGAA TATATACTTT TGAATCACTT ATAAAAATTA TTGCAAGGGG ATTCTGTTTA 
GAAGATTTTA CTTTCCTTCG GGATCCATGG AACTGGCTCG ATTTCACTGT CATTACATTT 
GCGTACGTCA CAGAGTTTGT GGACCTGGGC AATGTCTCGG CATTGAGAAC ATTCAGAGTT 
CTCCGAGCAT TGAAGACGAT TTCAGTCATT CCAGGCCTGA AAACCATTGT GGGAGCCCTG 
ATCCAGTCTG TGAAGAAGCT CTCAGATGTA ATGATCCTGA CTGTGTTCTG TCTGAGCGTA 
TTTGCTCTAA TTGGGCTGCA GCTGTTCATG GGCAACCTGA GGAATAAATG TATACAATGG 
CCTCCCACCA ATGCTTCCTT GGAGGAACAT AGTATAGAAA AGAATATAAC TGTGAATTAT 
AATGGTACAC TTATAAATGA AACTGTCTTT GAGTTTGACT GGAAGTCATA TATTCAAGAT 
TCAAGATATC ATTATTTCCT GGAGGGTTTT TTAGATGCAC TACTATGTGG AAATAGCTCT 
GATGCAGGCC AATGTCCAGA GGGATATATG TGTGTGAAAG CTGGTAGAAA TCCCAATTAT 
GGCTACACAA GCTTTGATAC CTTCAGTTGG GCTTTTTTGT CCTTGTTTCG ACTAATGACT 
CAGGACTTCT GGGAAAATCT TTATCAACTG ACATTACGTG CTGCTGGGAA AACGTACATG 
ATATTTTTTG TATTGGTCAT TTTCTTGGGC TCATTCTACC TAATAAATTT GATCCTGGCT 
GTGGTGGCCA TGGCCTACGA GGAACAGAAT CAGGCCACCT TGGAAGAAGC AGAACAGAAA 
GAGGCCGAAT TTCAGCAGAT GATTGAACAG CTTAAAAAGC AACAGGAGGC AGCTCAGGCA 
GCAACGGCAA CTGCCTCAGA ACATTCCAGA GAGCCCAGTG CAGCAGGCAG GCTCTCAGAC 
AGCTCATCTG AAGCCTCTAA GTTGAGTTCC AAGAGTGCTA AGGAAAGAAG AAATCGGAGG 
AAGAAAAGAA AACAGAAAGA GCAGTCTGGT GGGGAAGAGA AAGATGAGGA TGAATTCCAA 
AAATCTGAAT CTGAGGACAG CATCAGGAGG AAAGGTTTTC GCTTCTCCAT TGAAGGGAAC 
CGATTGACAT ATGAAAAGAG GTACTCCTCC CCACACCAGT CTTTGTTGAG CATCCGTGGC 
TCCCTATTTT CACCAAGGCG AAATAGCAGA ACAAGCCTTT TCAGCTTTAG AGGGCGAGCA 
AAGGATGTGG GATCTGAGAA CGACTTCGCA GATGATGAGC ACAGCACCTT TGAGGATAAC 
GAGAGCCGTA GAGATTCCTT GTTTGTGCCC CGACGACACG GAGAGAGACG CAACAGCAAC 
CTGAGTCAGA CCAGTAGGTC ATCCCGGATG CTGGCAGTGT TTCCAGCGAA TGGGAAGATG 
CACAGCACTG TGGATTGCAA TGGTGTGGTT TCCTTGGTTG GTGGACCTTC AGTTCCTACA 
TCGCCTGTTG GACAGCTTCT GCCAGAGGGA ACAACCACTG AAACTGAAAT GAGAAAGAGA 
AGGTCAAGTT CTTTCCACGT TTCCATGGAC TTTCTAGAAG ATCCTTCCCA AAGGCAACGA 
GCAATGAGTA TAGCCAGCAT TCTAACAAAT ACAGTAGAAG AACTTGAAGA ATCCAGGCAG 
AAATGCCCAC CCTGTTGGTA TAAATTTTCC AACATATTCT TAATCTGGGA CTGTTCTCCA 
TATTGGTTAA AAGTGAAACA TGTTGTCAAC CTGGTTGTGA TGGACCCATT TGTTGACCTG 
GCCATCACCA TCTGTATTGT CTTAAATACT CTTTTCATGG CCATGGAGCA CTATCCAATG 
ACGGACCATT TCAATAATGT GCTTACAGTA GGAAACTTGG TTTTCACTGG GATCTTTACA 
GCAGAAATGT TTCTGAAAAT TATTGCCATG GATCCTTACT ATTATTTCCA AGAAGGCTGG 
AATATCTTTG ACGGTTTTAT TGTGACGCTT AGCCTGGTAG AACTTGGACT CGCCAATGTG 
GAAGGATTAT CTGTTCTCCG TTCATTTCGA TTGCTGCGAG TTTTCAAGTT GGCAAAATCT 
TGGCCAACGT TAAATATGCT AATAAAGATC ATCGGCAATT CCGTGGGGGC TCTGGGAAAT 
TTAACCCTCG TCTTGGCCAT CATCGTCTTC ATTTTTGCCG TGGTCGGCAT GCAGCTCTTT 
GGTAAAAGCT ACAAAGATTG TGTCTGCAAG ATCGCCAGTG ATTGTCAACT CCCACGCTGG 
CACATGAATG ACTTCTTCCA CTCCTTCCTG ATTGTGTTCC GCGTGCTGTG TGGGGAGTGG 
ATAGAGACCA TGTGGGACTG TATGGAGGTT GCTGGTCAAG CCATGTGCCT TACTGTCTTC 
ATGATGGTCA TGGTGATTGG AAACCTAGTG GTCCTGAATC TCTTTCTGGC CTTGCTTCTG 
AGCTCATTTA GTGCAGACAA CCTTGCAGCC ACTGATGATG ATAATGAAAT GAATAATCTC 
CAAATTGCTG TGGATAGGAT GCACAAAGGA GTAGCTTATG TGAAAAGAAA AATATATGAA 
TTTATTCAAC AGTCCTTCAT TAGGAAACAA AAGATTTTAG ATGAAATTAA ACCACTTGAT 
GATCTAAACA ACAAGAAAGA CAGTTGTATG TCCAATCATA CAGCAGAAAT TGGGAAAGAT 
CTTGACTATC TTAAAGATGT AAATGGAACT ACAAGTGGTA TAGGAACTGG CAGCAGTGTT 
GAAAAATACA TTATTGATGA AAGTGATTAC ATGTCATTCA TAAACAACCC CAGTCTTACT 
GTGACTGTAC CAATTGCTGT AGGAGAATCT GACTTTGAAA ATTTAAACAC GGAAGACTTT 
AGTAGTGAAT CGGATCTGGA AGAAAGCAAA GAGAAACTGA ATGAAAGCAG TAGCTCATCA 
GAAGGTAGCA CTGTGGACAT CGGCGCACCT GTAGAAGAAC AGCCCGTAGT GGAACCTGAA 
GAAACTCTTG AACCAGAAGC TTGTTTCACT GAAGGCTGTG TACAAAGATT CAAGTGTTGT 
CAAATCAATG TGGAAGAAGG CAGAGGAAAA CAATGGTGGA ACCTGAGAAG GACGTGTTTC 
CGAATAGTTG AACATAACTG GTTTGAGACC TTCATTGTTT TCATGATTCT CCTTAGTAGT 
GGTGCTCTGG CATTTGAAGA TATATATATT GATCAGCGAA AGACGATTAA GACGATGTTG 
GAATATGCTG ACAAGGTTTT CACTTACATT TTCATTCTGG AAATGCTTCT AAAATGGGTG 
GCATATGGCT ATCAAACATA TTTCACCAAT GCCTGGTGTT GGCTGGACTT CTTAATTGTT 
GATGTTTCAT TGGTCAGTTT AACAGCAAAT GCCTTGGGTT ACTCAGAACT TGGAGCCATC 
AAATCTCTCA GGACACTAAG AGCTCTGAGA CCTCTAAGAG CCTTATCTCG ATTTGAAGGG 
ATGAGGGTGG TTGTGAATGC CCTTTTAGGA GCAATTCCAT CCATCATGAA TGTGCTTCTG 
GTTTGTCTTA TATTCTGGCT AATTTTCAGC ATCATGGGCG TAAATTTGTT TGCTGGCAAA 
TTCTACCACT GTATTAACAC CACAACTGGT GACAGGTTTG ACATCGAAGA CGTGAATAAT 
CATACTGATT GCCTAAAACT AATAGAAAGA AATGAGACTG CTCGATGGAA AAATGTGAAA 
GTAAACTTTG ATAATGTAGG ATTTGGGTAT CTCTCTTTGC TTCAAGTTGC CACATTCAAA 
GGATGGATGG ATATAATGTA TGCAGCAGTT GATTCCAGAA ATGTGGAACT CCAGCCTAAG 
TATGAAGAAA GTCTGTACAT GTATCTTTAC TTTGTTATTT TCATCATCTT TGGGTCCTTC 
TTCACCTTGA ACCTGTTTAT TGGTGTCATC ATAGATAATT TCAACCAGCA GAAAAAGAAG 
TTTGGAGGTC AAGACATCTT TATGACAGAA GAACAGAAGA AATACTATAA TGCAATGAAA 
AAATTAGGAT CGAAAAAACC GCAAAAGCCT ATACCTCGAC CAGGAAACAA ATTTCAAGGA 
ATGGTCTTTG ACTTCGTAAC CAGACAAGTT TTTGACATAA GCATCATGAT TCTCATCTGT 
CTTAACATGG TCACAATGAT GGTGGAAACA GATGACCAGA GTGAATATGT GACTACCATT 
TTGTCACGCA TCAATCTGGT GTTCATTGTG CTATTTACTG GAGAGTGTGT ACTGAAACTC 
ATCTCTCTAC GCCATTATTA TTTTACCATT GGATGGAATA TTTTTGATTT TGTGGTTGTC 
ATTCTCTCCA TTGTAGGTAT GTTTCTTGCC GAGCTGATAG AAAAGTATTT CGTGTCCCCT 
ACCCTGTTCC GAGTGATCCG TCTTGCTAGG ATTGGCCGAA TCCTACGTCT GATCAAAGGA 
GCAAAGGGGA TCCGCACGCT GCTCTTTGCT TTGATGATGT CCCTTCCTGC GTTGTTTAAC 
ATCGGCCTCC TACTCTTCCT AGTCATGTTC ATCTACGCCA TCTTTGGGAT GTCCAACTTT 
GCCTATGTTA AGAGGGAAGT TGGGATCGAT GACATGTTCA ACTTTGAGAC CTTTGGCAAC 
AGCATGATCT GCCTATTCCA AATTACAACC TCTGCTGGCT GGGATGGATT GCTAGCACCC 
ATTCTCAACA GTAAGCCACC CGACTGTGAC CCTAATAAAG TTAACCCTGG AAGCTCAGTT 
AAGGGAGACT GTGGGAACCC ATCTGTTGGA ATTTTCTTTT TTGTCAGTTA CATCATCATA 
TCCTTCCTGG TTGTGGTGAA CATGTACATC GCGGTCATCC TGGAGAACTT CAGTGTTGCT 
ACTGAAGAAA GTGCAGAGCC TCTGAGTGAG GATGACTTTG AGATGTTCTA TGAGGTTTGG 
GAGAAGTTTG ATCCCGATGC AACTCAGTTC ATGGAATTTG AAAAATTATC TCAGTTTGCA 
GCTGCGCTTG AACCGCCTCT CAATCTGCCA CAACCAAACA AACTCCAGCT CATTGCCATG 
GATTTGCCCA TGGTGAGTGG TGACCGGATC CACTGTCTTG ATATCTTATT TGCTTTTACA 
AAGCGGGTTC TAGGAGAGAG TGGAGAGATG GATGCTCTAC GAATACAGAT GGAAGAGCGA 
TTCATGGCTT CCAATCCTTC CAAGGTCTCC TATCAGCCAA TCACTACTAC TTTAAAACGA 
AAACAAGAGG AAGTATCTGC TGTCATTATT CAGCGTGCTT ACAGACGCCA CCTTTTAAAG 
CGAACTGTAA AACAAGCTTC CTTTACGTAC AATAAAAACA AAATCAAAGG TGGGGCTAAT 
CTTCTTATAA AAGAAGACAT GATAATTGAC AGAATAAATG AAAACTCTAT TACAGAAAAA 
ACTGATCTGA CCATGTCCAC TGCAGCTTGT CCACCTTCCT ATGACCGGGT GACAAAGCCA 
ATTGTGGAAA AACATGAGCA AGAAGGCAAA GATGAAAAAG CCAAAGGGAA ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The potential implication of SCN1A and CYP3A5 genetic variants on antiepileptic drug resistance among Egyptian epileptic children.
Seizure4175-80(2016 Oct)
Abo El Fotoh WM,Abd El Naby SA,Habib MS,ALrefai AA,Kasemy ZA

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SCN1A cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: